LOCUS       DAVAAL010000001        49128 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVAAL010000001 DAVAAL010000000
VERSION     DAVAAL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49128
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.001"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            603..1055
                     /locus_tag="VQM60_000001"
     CDS             603..1055
                     /locus_tag="VQM60_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET1079923.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1074..2411
                     /locus_tag="VQM60_000002"
     CDS             1074..2411
                     /locus_tag="VQM60_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET1079924.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIGVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2490..3374
                     /locus_tag="VQM60_000003"
     CDS             2490..3374
                     /locus_tag="VQM60_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET1079925.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKVVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3374..4207
                     /locus_tag="VQM60_000004"
     CDS             3374..4207
                     /locus_tag="VQM60_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET1079926.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4225..6318
                     /gene="nanB"
                     /locus_tag="VQM60_000005"
     CDS             4225..6318
                     /gene="nanB"
                     /locus_tag="VQM60_000005"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET1079927.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGMEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIGEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDACYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6331..7434
                     /locus_tag="VQM60_000006"
     CDS             6331..7434
                     /locus_tag="VQM60_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET1079928.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQNGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVVHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7608..8306
                     /locus_tag="VQM60_000007"
     CDS             7608..8306
                     /locus_tag="VQM60_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET1079929.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8325..9842
                     /locus_tag="VQM60_000008"
     CDS             8325..9842
                     /locus_tag="VQM60_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET1079930.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9967..11295
                     /locus_tag="VQM60_000009"
     CDS             9967..11295
                     /locus_tag="VQM60_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET1079931.1"
                     /translation="MKFRKLACTVLASAAVLGLAACGNSGGSKDAGKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDVKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDDFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATGWIKDNLLNNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYDDKRMETISSWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11548..12414
                     /locus_tag="VQM60_000010"
     CDS             11548..12414
                     /locus_tag="VQM60_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET1079932.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMIMGFITSFFNYSMTSFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNAIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAIMIAIVSF
                     VQFKVLGNDVEY"
     gene            12430..13269
                     /locus_tag="VQM60_000011"
     CDS             12430..13269
                     /locus_tag="VQM60_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET1079933.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13541..13993
                     /locus_tag="VQM60_000012"
     CDS             13541..13993
                     /locus_tag="VQM60_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET1079934.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNRFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14090..14434
                     /locus_tag="VQM60_000013"
     CDS             14090..14434
                     /locus_tag="VQM60_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1079935.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVTALIGRYIKLGTY
                     AVKYGISMARSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            14721..15365
                     /locus_tag="VQM60_000014"
     CDS             14721..15365
                     /locus_tag="VQM60_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET1079936.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15534..16451
                     /locus_tag="VQM60_000015"
     CDS             15534..16451
                     /locus_tag="VQM60_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET1079937.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTSSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTAAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEDDRPVVEAAAALIRETKERFL"
     gene            16469..17353
                     /locus_tag="VQM60_000016"
     CDS             16469..17353
                     /locus_tag="VQM60_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET1079938.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFAI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDGKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVEYVAAAHGDPVDQWNGRRIFKEATEGNKFCMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAKH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17369..18220)
                     /locus_tag="VQM60_000017"
     CDS             complement(17369..18220)
                     /locus_tag="VQM60_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET1079939.1"
                     /translation="MDKPDIATVIDSHFEEMTDLEQEIARYFLQAETIKDDLSSQHVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHEAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEVQLERIAQLIEEAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGTTPSILDSLLDAKEMGAKTVLFSSVPNKDSQAYTET
                     VLVATHSQSSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18396..20453
                     /locus_tag="VQM60_000018"
     CDS             18396..20453
                     /locus_tag="VQM60_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET1079940.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKVTTSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKEIA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVPTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEVDTYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELGNGGAKYSEGVYAVALNPKTGAVLSMSGLKHDLNTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQPIVFQGSAPIYSWYKLAYGSFPITAVEALEYSSN
                     AYMVQTALGIMGQTYQPNMFVGTSNLETAMGKLRATFGEYGLGAATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANDGVRVAPRIVEGIYGNNDKGGLGE
                     LIQAIDTKEINKVNISESDMAILHQGFYQVSHGTSPLTTGRAFSDGATVSISGKTGTG
                     ESYVAGGQEANNTNAVAYAPTENPQIAVAVVFPHNTNLTKNVGPAIARDIINLYNQHH
                     PMN"
     gene            20464..21060
                     /gene="recR"
                     /locus_tag="VQM60_000019"
     CDS             20464..21060
                     /gene="recR"
                     /locus_tag="VQM60_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET1079941.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMPDDDVNEFA
                     KNLLAAKRELTYCSICGRLTDEDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21236..22279
                     /locus_tag="VQM60_000020"
     CDS             21236..22279
                     /locus_tag="VQM60_000020"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET1079942.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDSIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22363..23736
                     /locus_tag="VQM60_000021"
     CDS             22363..23736
                     /locus_tag="VQM60_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET1079943.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENECK"
     gene            23723..24334
                     /locus_tag="VQM60_000022"
     CDS             23723..24334
                     /locus_tag="VQM60_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET1079944.1"
                     /translation="MNASDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDADEVAELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24425..25129
                     /locus_tag="VQM60_000023"
     CDS             24425..25129
                     /locus_tag="VQM60_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET1079945.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYRENK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25348..26721
                     /gene="ftsA"
                     /locus_tag="VQM60_000024"
     CDS             25348..26721
                     /gene="ftsA"
                     /locus_tag="VQM60_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET1079946.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26738..27997
                     /gene="ftsZ"
                     /locus_tag="VQM60_000025"
     CDS             26738..27997
                     /gene="ftsZ"
                     /locus_tag="VQM60_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET1079947.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            28002..28673
                     /locus_tag="VQM60_000026"
     CDS             28002..28673
                     /locus_tag="VQM60_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET1079948.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28683..29222
                     /locus_tag="VQM60_000027"
     CDS             28683..29222
                     /locus_tag="VQM60_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET1079949.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29222..29485
                     /locus_tag="VQM60_000028"
     CDS             29222..29485
                     /locus_tag="VQM60_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET1079950.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29482..30267
                     /locus_tag="VQM60_000029"
     CDS             29482..30267
                     /locus_tag="VQM60_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET1079951.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30276..31064
                     /gene="divIVA"
                     /locus_tag="VQM60_000030"
     CDS             30276..31064
                     /gene="divIVA"
                     /locus_tag="VQM60_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET1079952.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31412..34204
                     /locus_tag="VQM60_000031"
     CDS             31412..34204
                     /locus_tag="VQM60_000031"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET1079953.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
     gene            34607..34987
                     /locus_tag="VQM60_000032"
     CDS             34607..34987
                     /locus_tag="VQM60_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1079954.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGW
                     VGGILVIIGGSLFLATLKKFKSEE"
     gene            35181..35471
                     /locus_tag="VQM60_000033"
     CDS             35181..35471
                     /locus_tag="VQM60_000033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1079955.1"
                     /translation="MIESIRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKL
                     KESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            35522..36214
                     /locus_tag="VQM60_000034"
     CDS             35522..36214
                     /locus_tag="VQM60_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET1079956.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            36267..38678
                     /locus_tag="VQM60_000035"
     CDS             36267..38678
                     /locus_tag="VQM60_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET1079957.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            38928..39629
                     /locus_tag="VQM60_000036"
     CDS             38928..39629
                     /locus_tag="VQM60_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1079958.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            39631..42339
                     /locus_tag="VQM60_000037"
     CDS             39631..42339
                     /locus_tag="VQM60_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET1079959.1"
                     /translation="MIKRKTYWKDLIQSFTDSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDREIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMSQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(42435..42926)
                     /gene="tpx"
                     /locus_tag="VQM60_000038"
     CDS             complement(42435..42926)
                     /gene="tpx"
                     /locus_tag="VQM60_000038"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET1079960.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(43049..43978)
                     /gene="psaA"
                     /locus_tag="VQM60_000039"
     CDS             complement(43049..43978)
                     /gene="psaA"
                     /locus_tag="VQM60_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET1079961.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(44010..44858)
                     /locus_tag="VQM60_000040"
     CDS             complement(44010..44858)
                     /locus_tag="VQM60_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET1079962.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(44855..45577)
                     /locus_tag="VQM60_000041"
     CDS             complement(44855..45577)
                     /locus_tag="VQM60_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET1079963.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            45868..47760
                     /gene="pepO"
                     /locus_tag="VQM60_000042"
     CDS             45868..47760
                     /gene="pepO"
                     /locus_tag="VQM60_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET1079964.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVVAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(47883..48512)
                     /locus_tag="VQM60_000043"
     CDS             complement(47883..48512)
                     /locus_tag="VQM60_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET1079965.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
BASE COUNT        14955 a         8816 c        10829 g        14528 t
ORIGIN      
        1 cttgaaatac taaaaaaaaa agtatactct aattgaaaac ggtaacaaaa ctaatttaga
       61 gaatgaaata tagagtattt ctctcttaaa agtttttggt gaaacgagat gtagaaagga
      121 gatttagcca aagagtctat tagtgctaga ataatagatt agaattattt tagaaaaacg
      181 aagtgagcag cttataaatt caagtcccca aatagattca tactagtatc ttttgcaaaa
      241 aataaagggc gacttccgtc atgaatatca atttcatcta taaggaaggt agctaattga
      301 actaacttat ttattctgtt tgtcgctaga aaaatcagac ctccttgtga agattgagga
      361 gatactgaat gaaaatcaaa gaagaaacta gcaagctagt agcagattgc ccaaaacacc
      421 gccttgaggt tgtagataag actgacctat ataatccaag gtgaagcgac tgtggtttga
      481 agagattttc aaagagtata ggctagagag tagtgttttt atgtccttct agtagaaaat
      541 gctagacaga agaatgggga acttggatag gaaaaataga ttgagaaagg aggttagaag
      601 agatgattat tacaaaaatt agccgtttag gaacttatgt gggagtaaat ccacattttg
      661 caacattaat agattttcta gaaaaaacag gactagaaaa tttaacagaa ggttcgattg
      721 ctatcgatgg taatcgattg tttgggaatt gctttactta tctagcagat ggtcaagcag
      781 gggctttctt tgaaacacac caaaaatatt tggatattca tttagttttg gaaaacgaag
      841 aagccatggc tgttacatcg ccggaaaatg taagcgttac ccaagaatat gatgaagaga
      901 aagatattga attatacaca gggaaagtgg aacagttggt tcatttgaga gctggcgaat
      961 gcctcatcac ttttccagaa gatttacatc aacccaaggt tcgtataaat gatgaacctg
     1021 tgaaaaaagt tgtctttaaa gttgcgattt cttaatgtag aaagagaaga acgatgaaaa
     1081 aaatgagaaa gtttttatgt ctagctggaa ttgcgctagc ggctgttgcc ttggtagctt
     1141 gttcaggaaa aaaagaagct acaactagta ctgaaccacc aacagaatta tctggtgaga
     1201 ttacaatgtg gcactccttt actcaaggac cccgtttaga aagtattcaa aaatcagcag
     1261 atgctttcat gcaaaagcat ccaaaaacga aaatcaagat tgaaacattt tcttggaatg
     1321 acttctatac taaatggact acaggtttag caaatggaaa tgtgccagat atcagtacag
     1381 ctcttcctaa ccaagtaatg gaaatggtca actcagatgc tttggttccg ctaaatgatt
     1441 ctatcaagcg tattggacaa gataaattta acgaaactgc cttaaatgaa gcaaaaatcg
     1501 gagatgatta ctactctgtt cctctttatt cacatgcaca agtcatgtgg gttagaacag
     1561 atttgttaaa agaacataat attggggttc ctaaaacttg ggatcaactc tatgaagctt
     1621 ctaaaaaatt gaaagaagct ggagtttatg gcttgtctgt tccgtttgga acaaatgact
     1681 taatggcaac acgtttcttg aacttctacg tacgtagtgg tggaggaagc ctcttaacaa
     1741 aagatcttaa agcagacttg acaagccaac ttgctcaaga tggtattaaa tactgggtta
     1801 aattgtataa agaaatctca cctcaagatt ctttgaattt taatgtcctt caacaagcta
     1861 ccttgttcta tcaaggaaaa acagcatttg actttaactc tggcttccat atcggaggaa
     1921 ttaatgccaa cagtcctcaa ttgattgatt cgattgatgc ttatcctatt ccaaaaatca
     1981 aagagtctga taaagaccaa ggaattgaaa cctcaaacat tccaatggtt gtttggaaaa
     2041 attcaaaaca tccagaagtt gctaaagcat tcttagaagc actttataat gaagaagact
     2101 acgttaaatt ccttgattca actccagtag gtatgttgcc aactattaag gggattagcg
     2161 attctgcagc ctataaagaa aatgaaactc gtaagaagtt taaacatgct gaagaagtaa
     2221 ttactgaagc tgttaaaaaa ggtactgcta ttggttatga aaatggacca agtgtacaag
     2281 ctggtatgtt gactaaccaa cacattattg aacaaatgtt ccaagatatc attacaaatg
     2341 gaacagatcc tatgaaagca gcaaaagaag cagaaaaaca attaaacgat ttatttgagg
     2401 ctgttcagta gatgtaaaag actagaaaat aggtgggcta gtgagctgaa aagctctagc
     2461 ccaatcttgt aaaagaaggg agaaggagaa tggttaaaga acgtaattta actcgctgga
     2521 tatttgtttt gccagctatg attatcgtag gattactctt tgtttatccg tttttctcga
     2581 gtatttttta tagctttacc aataagcatt tgattatgcc taattataaa tttgttggtt
     2641 tggctaacta taaagttgtg ctatcagatc ccaacttctt taatgcgttc tttaattcaa
     2701 ttaagtggac cgttttctca ttagttggtc aagttttagt agggtttgta ttggctttag
     2761 ctcttcacag agtacgccac ttcaagaaat tatataggac cttattgatt gttccttggg
     2821 catttcctac catcgtcatt gccttttctt ggcagtggat tctaaacggg gtttatggct
     2881 acttacctaa tctaatcgta aaattaggtt taatggaaca tacacctgca tttttgacag
     2941 atagtacatg ggcattccta tgtttggtgt ttatcaacat ttggtttgga gcaccaatga
     3001 ttatggttaa tgtgctttca gctttgcaaa cagtaccaga agaacaattt gaggctgcta
     3061 agatagatgg tgcttcaagt tggcaggtgt tcaagtttat cgtctttcca catattaaag
     3121 tggttgtagg acttctagtt gttttgagaa ctgtatggat ctttaataac tttgacatta
     3181 tctacctcat tactggtggt ggaccagcca atgctacaac gacgcttcca atttttgctt
     3241 acaacctggg ctggggaact aaattgttgg gtcgtgcttc agcagttaca gtattgctct
     3301 ttatcttctt ggtggcgatt tgctttatct actttgctat catcagtaag tgggaaaagg
     3361 agggtagaaa ataatgaaga agaaatccag tatttattta gatattctct cacatgtact
     3421 tttagttggt gcgaccatcg ttgcaatttt cccattggta tggattatca tatcttctgt
     3481 caaagggaaa ggggaattaa ctcagtatcc aacacgattt tggcctgaac agtttacatt
     3541 agattatttc actcatgtta tcaacgattt gcacttcatt gataacattc gaaacagttt
     3601 aatcattgcc ttggctacaa ccctcattgc gattattatt tctgctatgg cagcctatgg
     3661 tattgttcga ttctttccta aattgggagc aatcatgtcg agactactcg tcattaccta
     3721 cattttccca ccaattttgt tagcaattcc ctattcaatt gccattgcta aagttgggtt
     3781 aacaaatagt ttatttggct tgatgatggt ttatctatct tttagtgttc catatgcagt
     3841 ttggctctta gttggatttt tccaaacagt tccaattgga attgaagaag cggctagaat
     3901 tgatggtgca aataaatttg ttacgtttta taaagttgtg ctaccgattg tagcaccagg
     3961 tattgtagca acagctattt atacatttat caatgcttgg aatgaattcc tgtatgcctt
     4021 gattttgatt aacaatacag gaaagatgac agtagcagta gcccttcgtt cacttaatgg
     4081 ttcagaaata ctagactggg gagatatgat ggcagcgtct gttattgtag ttcttccatc
     4141 aattattttc ttctctatca tccaaaataa gattgcaagt ggattatcag aaggatctgt
     4201 gaagtagacg aaagaaggaa aaaaatgaat aaaagaggtc tttattcaaa actaggaatt
     4261 tctgttgtag gcattagtct tttaatggga gtccccactt tgattcatgc gaatgaatta
     4321 aactatggtc aactgtccat atctcctatt tttcaaggag gctcatatca actgaacaat
     4381 aagagtatag atatcagccc tttgttatta gataaattgt ctggagagag tcagacagta
     4441 gtaatgaaat ttaaagcaga tgaaccaaac tctcttcaag ctttgtttgg cctatctaat
     4501 agtaaagcag gctttaaaaa taattacttt tcaattttca tgagagattc tggtgagata
     4561 ggtgtagaaa taagagacgc ccaaaaggga ataaattatt tattttccag accagcttca
     4621 ttatggggaa aacataaagg acaggcagtt gaaaatacac tagtatttgt atctgattct
     4681 aaagataaaa catacacaat gtatgttaat ggaatggaag tgttctctga aacagttgat
     4741 acatttttgc caatttcaaa tataaatggt atagataagg caacactagg agctgttaat
     4801 cgtgaaggta aggaacatta cctcgcaaaa ggaagtattg gtgaaatcag tctatttaac
     4861 aaagcaatta gtgatcagga agtttcaact attcccttgt caaatccatt tcagttaatt
     4921 ttccaatcag gagattctac tcaagctaac tattttagaa taccgacact atatacatta
     4981 agtagtggaa gagttctatc aagtattgat gcatgttatg gtgggactca tgattctaaa
     5041 agtaagatta atattgccac ttcttatagt gatgataatg ggaaaacgtg gagtgagcca
     5101 atttttgcta tgaagtttaa tgactatgag gagcagttag tttactggcc acgagataat
     5161 aaattaaaga atagtcaaat tagtggaagt gcttcattca tagattcatc cattgttgaa
     5221 gataaaaaat ctgggaaaac gatattacta gctgatgtta tgcctgcggg tattggaaat
     5281 aataatgcaa ataaagccga ctcaggtttt aaagaaataa atggtcatta ttatttaaaa
     5341 ctaaagaaga atggagataa cgatttccgt tatacagtta gagaaaatgg tgtcgtttat
     5401 gatgaaacaa ctaataaacc tacaaattat actataaatg ataagtatga agttttggag
     5461 ggaggaaagt ctttaacagt cgaacaatat tcggttgatt ttgatagtgg ctctttaaga
     5521 gaaaaacata atggaaaaca ggtccctatg aatgttttct acaaagattc gttatttaaa
     5581 gtgactccta ctaattatat agcaatgaca actagtcaga atagaggaga gagttgggaa
     5641 caatttaagt tgttgcctcc gttcttagga gaaaaacata atggaactta cttatgtccc
     5701 ggacaaggtt tagcattaaa atcaagtaac agattgattt ttgcaacata tactagtgga
     5761 gaactaacct atctcatttc tgatgatagt ggtcaaacat ggaagaaatc ctcagcttca
     5821 attccgttta aaaatgcaac agcagaagca caaatggttg aactgagaga tggtgtgatt
     5881 agaacattct ttagaaccac tacaggtaag atagcttata tgactagtag agattctgga
     5941 gaaacatggt cggaagtttc gtatattgat ggaatccaac aaacttcata tggcacacaa
     6001 gtatctgcaa ttaaatactc tcaattaatt gatggaaaag aagcagtcat tttgagtaca
     6061 ccaaattcta gaagtggccg taagggaggt caattagttg tcggtttagt caataaagaa
     6121 gatgatagta ttgattggaa ataccactat gatattgatt tgccttcgta tggttatgcc
     6181 tattctgcga ttacagaatt gccaaatcat cacataggtg tactgtttga aaaatatgat
     6241 tcgtggtcga gaaatgaatt gcatttaagc aatgtagttc agtatataga tttggaaatt
     6301 aatgatttaa caaaataaag gagaaaaaac atggttaaat acggtgttgt tggagcaggg
     6361 tattttggag ctgaattggc tcgctatatg caaaagaatg atggagcaga gattactctt
     6421 ctctatgatc cagataatgc agaggcgatt gcagaagaat tgggagcaaa agtagcaagt
     6481 tccttagatg agttggtttc tagcgatgaa gtagattgtg ttatcgtcgc aactccaaat
     6541 aatcttcata aggaaccggt tattaaggct gcacagaatg gtaaaaatgt tttctgtgaa
     6601 aaaccaattg cgctttctta tcaagattgt cgcgagatgg tagatgcatg taaagaaaac
     6661 aatgtaacct ttatggcagg acatattatg aatttcttta atggtgttca tcatgcaaaa
     6721 gaactcatta atcaaggagt tatcggagac gttctatatt gtcatacagc tcgtaatggt
     6781 tgggaagaac aacaaccgtc agtatcatgg aaaaaaattc gtgaaaaatc aggtggtcac
     6841 ttgtatcacc acatccatga attggattgc gttcaattcc ttatgggggg catgcctgaa
     6901 actgtaacca tgacaggtgg aaatgtggtc catgaaggtg aacatttcgg tgatgaagat
     6961 gatatgattt ttgtcaatat ggaattttct aataagcgtt ttgccttgtt agaatggggt
     7021 tcagcttatc gttggggtga acattatgtc ttaatccaag gaagcaaagg tgccatccgc
     7081 ttagacttat tcaactgtaa aggaactctt aagctagatg ggcaagaaag ctatttcttg
     7141 attcacgaat cgcaagaaga agatgatgat cggactcgta tctatcatag tacagagatg
     7201 gatggagcaa ttgcttatgg taaaccaggt aaacgtactc cattatggct atcatctgtc
     7261 attgataaag aaatgcgcta tctgcatgag attatggaag gagctccagt atcagaagaa
     7321 tttgcaaaac ttttgacagg tgaagctgcc ctagaagcaa ttgctactgc agatgcttgt
     7381 acccagtcta tgtttgaaga tcgcaaagta aaattgtcag aaattgtaaa ataaattttg
     7441 gtattctcct atttataggt cgacttgctc ctctgaaagt acttttagag gagctgtttg
     7501 actttgctag tttttgaaac tgaaatctat tatactacaa actattgaaa gcgttttaat
     7561 tttaaggtat aataatctca tagaaataaa gaaaaggagg aaagaggatg ccacagatta
     7621 gcaaagaagc cttgattgag caaatcaaag atggaatcat cgtttcttgt caggctcttc
     7681 ctcatgaacc gctttataca gaagcgggag gggtgattcc cttgctggtc aaagcggctg
     7741 agcaaggtgg ggcagtcggt atccgagcaa acagtgttcg cgatatcaag gaaattaagg
     7801 aagtcactaa acttccaatc attgggatta tcaaacgtga ttatccacct caggaaccct
     7861 tcatcacggc tactatgaaa gaagttgatg aattggcaga actggacatc gaggtgattg
     7921 ctctggattg taccaagcgt gaacgctacg atggtttgga aattcaagag ttcattcgtc
     7981 aggttaagga gaaatatcct aatcagcttt tgatggctga tactagtatc ttcgaagaag
     8041 ggctagcagc tgtagaagca ggaattgact ttgtcggaac aaccttatca ggctacacat
     8101 cctacagtcc aaaagtagac ggtccagatt ttgaattgat taagaaactc tgtgatgccg
     8161 gtgtagatgt cattgcagaa ggaaaaattc atacaccaga acaagccaaa caaatccttg
     8221 aatatggagt gcgaggcatc gttgttggtg gcgctattac tagaccaaaa gagattacag
     8281 aacgcttcgt tgctagtctt aaataagatg tgagggggag ttttatgttt aaagttttac
     8341 aaaaagttgg aaaagctttt atgttaccta tagctatact tcctgcagca ggtctacttt
     8401 tggggattgg tggtgcactt tcaaacccaa ccacgatagc aacttatcca atactagaca
     8461 atagtatttt tcaatcgata ttccaagtaa tgagctctgc aggagaggtt gtattcagta
     8521 atttgtcact acttctctgt gtgggattat gtattggctt agcgaaacga gataaaggaa
     8581 ccgctgcgtt agcaggagta actggttact tagttatgac tgcaacgatc aaagctttgg
     8641 taaaactttt tatggcagaa ggatctgcaa ttgatactgg agttattgga gcattagttg
     8701 tcggaatagt tgccgtatat ttgcacaacc gatataacaa tattcaatta ccttccgctt
     8761 taggattctt tggaggttca cgcttcgttc ctattgttac atcgttctct tctatcttga
     8821 ttggctttgt cttctttgtt atttggccac ctttccaaca acttcttgtt tctacaggtg
     8881 gatatatttc tcaggcgggt ccaattggaa cttttctata tggattttta atgagacttt
     8941 ctggagcagt aggcttacat catataattt accctatgtt ttggtatact gaacttggtg
     9001 gtgttgaaac tattgcagga caaacagtgg ttggagctca aaaaatattt tttgctcaat
     9061 tagccgatcc ggcccattct ggattattta cagaaggaac aaggtttttt gcaggtcgtt
     9121 tctcaacaat gatgttcggt ttaccggctg cctgtttagc gatgtaccat agtgttccta
     9181 aaaatcgtcg taaaaaatac gcgggtttgt tttttggagt tgctttaaca tcttttatta
     9241 ccggtattac agaaccaatt gaatttatgt ttctattcgt cagtccggtt ctatatgttg
     9301 ttcacgcatt ccttgatggt gttagcttct ttattgcaga cgtcttaaat atttcaatag
     9361 gaaacacatt ttcaggaggt gtaatcgatt tcacattatt tggaattttg caggggaacg
     9421 ctaagacgaa ttgggttctt cagattccaa ttggacttat ttggagtgtt ttgtattata
     9481 ttatttttag atggttcatt actcaattca acgttctaac gccaggacga ggagaagaag
     9541 tagattctaa agaaatttct gaatccgcag attcaacttc aaatactgca gattatttaa
     9601 aacaggatag cctacaaatt atcagagcct tgggtggatc aaataatata gaagatgtag
     9661 atgcttgtgt gacacgttta cgtgtagctg taaaagaagt taatcaagtt gataaagcac
     9721 ttttaaaaca aattggtgca gttgatgtct tagaagtgaa gggtggcatt caagcaatct
     9781 atggagcaaa agcaatctta tataaaaata gtattaatga aattttaggt gtagatgatt
     9841 aagtacttac tgacttaata aaaaacagag gagagtgatg gatgagtagg atgaaatgaa
     9901 atcgcatata agaaataaag aactcattat ccaagttgga tacgcttatt acataggaga
     9961 atacagatga aatttagaaa attagcttgt acagtacttg cgagtgctgc ggttcttggt
    10021 cttgctgctt gtggcaattc tggcggaagt aaagatgctg gcaaatcagg tggtgacggt
    10081 gccaaaacag aaatcacttg gtgggcattc ccagtattta cccaagaaaa aactggtgac
    10141 ggtgttggaa cttatgaaaa atcaatcatc gaagcgtttg aaaaagccaa tccagatgta
    10201 aaagtgaaat tggaaaccat cgacttcaag tcaggtcctg aaaaaatcac aacagctatc
    10261 gaagctggaa cagctccaga cgtactcttt gatgcaccag gacgtatcat ccaatacggt
    10321 aaaaacggta aattggctga gttgaacgac ctcttcacag atgactttgt taaagatgtc
    10381 aacaatgaaa acatcgtaca agcaagtaaa gctggagaca aggcttacat gtatccaatt
    10441 agttctgccc cattctacat ggcaatgaac aagaaaatgt tagaagatgc tggagttgca
    10501 aaccttgtaa aagaaggttg gacaactgat gattttgaaa aagtattgaa agcacttaaa
    10561 gacaagggtt acacaccagg ttcattgttc agttctggtc aagggggaga ccaaggaaca
    10621 cgtgccttta tctctaacct ttatagcggt tctgtaacag atgaaaaagt tagcaaatat
    10681 acaactgatg atcctaaatt cgtcaaaggt cttgaaaaag caactggctg gattaaagac
    10741 aatttgctca ataatggttc acaatttgac ggtggggcag atatccaaaa ctttgccaac
    10801 ggtcaaacat cttacacaat cctttgggca ccagctcaaa atggtatcca agctaaactt
    10861 ttagaagcaa gtaaggtaga agtggtagaa gtaccattcc catcagacga aggtaaacca
    10921 gctcttgaat accttgtaaa cggatttgca gtattcaaca acaaagacga caagaaagtt
    10981 gcagcatcta agaaattcat ccaatttatc gcagatgaca aagagtgggg tccaaaagac
    11041 gtagttcgta caggtgcctt cccagttcgt acatcatttg gcaaacttta tgatgacaaa
    11101 cgtatggaaa caatcagcag ctggactcaa tactactcac catactacaa cactattgat
    11161 ggatttgctg aaatgagaac actttggttc ccaatgttgc aatctgtatc aaatggtgac
    11221 gaaaaaccag cggacgcttt gaaagccttc actgaaaaag cgaacgaaac aatcaaaaaa
    11281 gctatgaaac aatagtcctt agttattcta taaaaagtag ttttttaaag aacctaagag
    11341 tgtatacccc cttttccctc tacacagata gtgtaagaaa aggggggctt ttgtttaaaa
    11401 cgtaagaaat tgtcacgaaa ttaaaatgaa gttcttacat aagtgaatca taaaaaattt
    11461 cattttgatt ttaaaacagt tcaagaaagt caaaaaatta ttctatttga aagagaggtg
    11521 ccgactgtga aagtcaataa aatccgtatg cgggaaacag tgatttccta tgctttccta
    11581 gcaccagtgt tgttcttctt tgtcatcttt gtattggctc caatgattat gggctttatt
    11641 acaagtttct ttaactactc tatgactagc tttgaatttg tgggcttaga taactatatc
    11701 cgtatgttta aagatcctgt ctttacaaaa tctttgatta acacagttat tttggttatt
    11761 ggatctgtgc cagttgttgt tctattctca ctctttgtag cgtcacagac ctaccatcaa
    11821 aatgctatcg ctagatcatt ctatcgtttc gtattcttcc ttccagttgt tacaggtagt
    11881 gttgccgtaa cagttgtatg gaaatggatt tatgacccac tatcagggat tctaaacttt
    11941 gtccttaagt ccagccacat catcagccaa aacatttctt ggttgggaga taaaaactgg
    12001 gcattgatgg cgattatgat tattctcttg accacttcag ttggtcagcc catcatcctt
    12061 tatatcgctg ccatggggaa tattgacaat tcactggttg aagcggcgcg tgttgatggt
    12121 gcaactgagt ttcaagtttt ttggaagatt aaatggccaa gccttcttcc aacaactctt
    12181 tatattgcaa tcatcacaac aattaactca ttccagtgtt ttgccttgat tcagcttttg
    12241 acatctggtg gtccaaacta ctcaacaagt accttgatgt actaccttta cgaaaaagcc
    12301 ttccaattga cagaatacgg ctatgccaac acaattggtg tcttcttggc aatcatgatt
    12361 gctatcgtaa gctttgttca atttaaagta cttggaaacg acgtagaata ctaaagaaag
    12421 gagacagcta tgcaatctac agaaaaaaaa ccattaacag cctttactgt tatttcaaca
    12481 atcattttgc tcttgttgac tgtgctgttc atctttccat tctactggat tttgacaggg
    12541 gcattcaaat cacaacctga tacaattgtt attcctcctc agtggttccc taaaatgcca
    12601 accatggaaa acttccaaca actcatggtg cagaaccctg ccttgcaatg gatgtggaac
    12661 tcagtattta tctcattggt aaccatgttc ttagtctgtg caacctcatc tctagcaggt
    12721 tatgtattgg ctaaaaaacg tttctatggt caacgcattc tatttgctat ctttatcgct
    12781 gctatggcgc ttccaaaaca agttgtcctt gtaccattgg tacgtatcgt caacttcatg
    12841 ggaatccacg atactctctg ggcagttatc ttgcctttga ttggatggcc attcggtgtc
    12901 ttcctcatga aacagttcag tgaaaatatc cctacagagt tgcttgaatc agctaaaatc
    12961 gacggttgtg gtgagattcg taccttctgg agtgtagcct tcccgattgt gaaaccaggg
    13021 tttgcagccc ttgcaatctt taccttcatc aatacttgga atgactactt catgcaattg
    13081 gtaatgttga cttcacgtaa caatttgacc atctcacttg gggttgcgac catgcaggct
    13141 gaaatggcaa ccaactatgg tttgattatg gcaggagctg cccttgctgc tgttccaatc
    13201 gtcacagtct tcctagtctt ccaaaaatcc ttcacacagg gtattactat gggagcggtc
    13261 aaaggataat actctgagaa aatctcttca aactacgtca gcgtcacctt gccatactta
    13321 agtattgcct gcggttagct tcctagtttg ttcttcaatt ttcattgagt ataggaaaat
    13381 caatctatca agatacagaa gtatatttta tagatttaga gaatatagag gttataagtg
    13441 tctacaaaat ggagggtatg cagttacttt atgaagtttt gtcagacact tataaactta
    13501 agaatggttt tagttaacta tcagaaacga aggaaagagt atgatttttg acgatttgaa
    13561 aaacatcacc ttttacaaag ggattcatcc caatttagac aaggctatcg actatctcta
    13621 ccaacatcgt aaagattcat tcgaattagg aaagtatgag attgatggag ataaagtctt
    13681 tctagttgtt caggaaaatg tcctcaatca agttgagaat aatcgatttg aacaccataa
    13741 gaactatgca gatttgcatt tgctgataga agggcatgaa tattcgagct acggttcacg
    13801 tatcaaagac gaggcagtag cattcgacga agcgagtgac attggctttg ttcattgtca
    13861 tgaacactac ccactcttgt tgggttatca caattttgcg attttcttcc caggtgagcc
    13921 acatcagcca aatggttatg caggcatgga agaaaaggtt cgaaaatatc tctttaaaat
    13981 tttgattgat taaaaatagg atgaattgtt tttttgtaaa gctttgataa tactctacca
    14041 tgaaattgat ctttgtgagg tagagaaatg agaataaaat atttaaaaat tggtatcttc
    14101 taagtatgct gcaagagcta gtttcttaga tggacagggg attacagttg atgagatggc
    14161 ttggataatt aggggcattg tgactgcatt gattggtaga tacataaaat taggtactta
    14221 tgcagttaag tatggtatta gtatggcgcg ctcgatctta agtagggcag ctgcaactgc
    14281 ggcagcaaga gtaggattat tgactaagat ttttggatgg attttacgaa tagctgtgct
    14341 cgcagctgat gtatatggta attttgccaa caatattgct gtagcttggg atgcacatga
    14401 taaaatccct aacaatggtc gtataaactt ttaaaatgcg agaatgaaag cactttgtat
    14461 ttttttattg tatatgttag cttggacagt gcttgcaatg ataatccgtg gagggctaga
    14521 tggatttgat gaacacactt ggcgtactat tttaattgct tcgctgtccg ggatatatat
    14581 agattatatg tccatagata aaagtagaaa aaagtccaaa agaaaaaaga aaaaatagat
    14641 ttgttcatgg tagggactta tgaaagcttt actgacaaaa aagaaaacag tttacaaaga
    14701 aaaatgatgg aggagcaaac atggcacaaa aaggagtaag ccttatcaag gcagcatttg
    14761 atacagataa ctttctcatg cgttttagtg agaaagtctt ggacatcgtg acagccaatc
    14821 ttctttttgt cgtctcttgt ttgcccatcg tgacgattgg agtggctaaa atcagcctct
    14881 acgagaccat gttcgaagtt aagaagagca gacgggtgcc tgtttttaaa atctatctaa
    14941 gatctttcaa gcaaaatctg aaactaggtc ttcagctggg tttaatggag ttaggaattg
    15001 tgtttcttac cctttcagat ctctatcttt tctggggtca aacagctctg cccttccaat
    15061 tgctgaaagc catttgtcta ggcattctga ttttccttac gattgtgatg ttggctagct
    15121 atccaatcgc ggcacgttat gatttaactt ggaaagaagt gttacaaaaa gggcttatct
    15181 tggctagttt taactttcct tggttcttcc tcatgttagc cattcttgtc ctcattgtga
    15241 tggttcttta tctgtccgcc ttcagtctac tcttaggtgg ctcagtcttc ctactttttg
    15301 ggtttggact attggtcttt atccagactg gattgatgga gaaaattttc gcaaaatacc
    15361 aataggagcc ttatttctga aactactttc aaaggctcca aacgctattc tataagcgag
    15421 aaactaaaat ctaagtgtct ataagatatt gaaagccttt tcggtaaagt gtatactaaa
    15481 gaagtaaaga aaaatctgct ttagcagaaa ataaaaataa taggagaaaa tctatgtcag
    15541 atttgaaaaa atacgaaggt gtcattccag ccttctacgc atgttatgat gagcaaggag
    15601 aagtaagtcc agagcgtacg cgtgccttgg ttcaatactt cattgataaa ggtgttcaag
    15661 gtctttatgt caatggttct tctggtgaat gtatctacca aagcgttgaa gatcgcaagt
    15721 tgattttgga agaagtcatg gcggtagcca aaggtaaatt gaccattatt gcccatgttg
    15781 cttgcaataa tactaaagat agtatggaac ttgctcgcca tgctgaaagc ttgggagtag
    15841 atgctattgc aacgattcca ccaatttact tccgcttgcc agaatactca gttgccaaat
    15901 actggaacga tattagttct gcagctccaa acacagacta cgtgatttac aacattcctc
    15961 aattggcagg ggttgctttg acttcaagcc tttacacaga aatgttgaaa aatcctcgtg
    16021 ttatcggtgt taagaactct tcaatgccag ttcaagatat ccaaaccttt gtcagccttg
    16081 gcggggaaga ccatattgtc tttaacggtc cagatgaaca gttcctaggt ggccgcctca
    16141 tgggtgctaa ggctggtatc ggtggtactt atggtgctat gccagaactc ttcttgaaac
    16201 tcaatcagtt gattgcggat aaagatttag aaacagcgcg tgaattgcag tatgctatca
    16261 acgcaatcat tggtaaactc actgctgcac atggaaatat gtacggtgtc atcaaagaag
    16321 ttttgaagat caatgaaggc ttgaatattg gttctgttcg ttcaccattg acaccagtga
    16381 ctgaagacga tcgtccagtt gtagaagcgg ctgcagcctt gattcgtgaa accaaggagc
    16441 gcttcctcta atctaaaagg aggtatttat gacatactac gttgcaattg atatcggtgg
    16501 aactaacatc aagtatggtt tggttgatca agaggggcaa cttcttgaat cacatgaaat
    16561 gccaacagag gcgcataagg gtggacctca tatcttacaa aagaccaaag atatcgtagc
    16621 tagttattta gaaaaaggcc cagtagcagg tgttgccata tcttctgctg ggatggtgga
    16681 tccggataag ggtgagattt tctatgctgg gccgcaaatc cctaactacg caggcaccca
    16741 gttcaaaaag gaaatcgaag aaagctttgc tattccttgt gagattgaaa atgatgtcaa
    16801 ctgtgcaggt ctggctgagg cagtatctgg ttcaggtaag ggagcaagtg tcaccctttg
    16861 cttgaccatt ggaacaggta ttggtggttg tttgattatg gatgggaaag tcttccatgg
    16921 atttagcaat tcagcctgtg aagttggtta tatgcatatg caggatggag cgtttcaaga
    16981 tttggcttct acaacagcct tggtggagta tgtagcagca gctcatggag atccagttga
    17041 ccagtggaat ggtcgtcgta tctttaagga agctacggaa ggcaataaat tctgcatgga
    17101 aggtattgac cgcatggtag actatctagg aaaaggtctg gcaaatattt gctacgttgc
    17161 caatccagaa gtggtcattc tcggtggcgg tatcatggga caagaggcga ttctcaaacc
    17221 gaaaatccgc acagccttga aagaggcttt ggtaccaagt ttggctgaga aaacaagatt
    17281 agaattcgcc aagcaccaaa ataccgcagg catgttgggt gcttattatc attttaaaac
    17341 aaaacaatcc tagtttggct tagccaaact aggattttct gacacgtttt tgtctacgat
    17401 agccgttgag ttttttattt tcccagtagc tattaaagat tttctcctta ctttcacgat
    17461 tgatttccaa aaagtaggcg taaatcaaat cgataaagaa gagcatagga agttgagcgg
    17521 atatacgttg gatgtaagaa gactggctgt gggtggctac aagaacagtc tctgtgtagg
    17581 cctgactatc tttattggga acacttgaaa aaagtacagt ctttgcgccc atctccttag
    17641 cgtctaatag actatctaaa atagaaggag ttgtgccaga aagtgagaaa ccgagtacga
    17701 gacaattttc atccataata ctggttgtcc aagcaaagcc gtcttggtct gtcaaagctt
    17761 cgcagacaac acccagacgc atgaagcgca atttcatttc acgagcaaca agaccagaac
    17821 tccctgttcc gaagaagtag atacgctcag cctcttcgat tagctgggca attcgttcta
    17881 gttggacttc gtcaatcaag tcttgtgttt gttccctcat attgctataa cttctgagga
    17941 ctcgtttggt cagtggactg tgcttggaga cttggttggc ttgattttct gcttcatgtt
    18001 ggtattggaa aataaattct cggtagccag taaagccaca ctttttagca aagcgggtca
    18061 aagcagcctg agagatatgt aatttttgag tgacatgttg ggaagataaa tcatctttaa
    18121 tcgtttcagc ttgcaaaaaa tagcgagcga tttcttgctc taggtctgtc atttcttcaa
    18181 agtgtgaatc aatgacagtt gcgatatctg gtttgtccat agggaaggct cctttaaatg
    18241 agtcgtgttg gaaaaagact agattattga acttttacat tcattataac atataatata
    18301 gggatgaata aataaaaacg aaaaaaagca tctttctttt ccataatttt ctgctcaaat
    18361 tgtggtacaa ttaagagtaa gattttaagt tagaaatgag actgatttgt atgagaaaat
    18421 ttaacagcca ttcgattccg attcggctta atttattgtt ttcaatcgtc attttactct
    18481 ttatgaccat tattggtcgt ttgttgtata tgcaggtttt gaacaaggat ttttacgaaa
    18541 aaaagctagc ctcagctagt cagaccaagg tcacaaccag ttctgctcgt ggggaaatct
    18601 atgatgctag tggaaaacct ttggtagaaa atacgttaaa gcaggttgtt tcctttacgc
    18661 gtagcaataa aatgacggct acagacttaa aagaaatagc taaaaagtta ctgacttatg
    18721 tgagcatcag ttcgccaaat ttgacagaac gccagctggc tgattactat ttggctgatc
    18781 ctgaaatcta taaaaaaaca gtggaagctc tcccaagtga gaaacgattg gattcagatg
    18841 gcaatcgcct atccgaatca gaactgtata acaatgcggt cgatagtgtc ccaacaagtc
    18901 aactaaacta tacagaggat gaaaagaaag aaatctatct ttttagtcag ttaaatgctg
    18961 ttggaaactt tgcgacagga accattgcga cagatcctct aaatgattct caggtggctg
    19021 ttattgcctc tatttcaaag gagatgcctg gcattagtat ttctacttct tgggatagaa
    19081 aggttttaga aacctctctt tcttctatag taggtagtgt atccagtgaa aaagctggtc
    19141 tcccagcgga agaagtggat acctatctta aaaaaggtta ttctctaaat gatcgtgttg
    19201 gaacctccta tttggaaaag caatatgaag agaccttaca agggaaacgc tcggtaaaag
    19261 aaatccatct ggataaatat ggcaacatgg aaagtgtgga tacaattgag gaaggtagta
    19321 agggaaacaa tatcaaactg accattgatt tggcctttca agatagtgtg gatgctttgt
    19381 tgaaaagtta tttcaattcc gagctaggaa atgggggagc caagtattct gagggtgttt
    19441 atgcagttgc tttgaatccc aaaacaggtg cggttttgtc tatgtcaggg ctcaaacatg
    19501 acctgaacac gggagagttg actcctgatt ccttgggaac ggtaaccaat gtctttgtcc
    19561 cagggtcagt agttaaggcc gctaccatca gctcaggttg ggaaaatggt gttttatcag
    19621 gaaaccaaac cttaacagat cagcctattg ttttccaagg ttcagctcca atttattctt
    19681 ggtataaatt ggcatatgga tcttttccta ttacagctgt ggaagccttg gagtattcat
    19741 ctaatgctta catggttcaa accgctcttg gaatcatggg ccagacctat caaccaaata
    19801 tgtttgttgg aaccagcaat ttggaaacag ctatgggaaa acttcgtgcg acctttggcg
    19861 aatatggctt gggggctgcg accggaattg acctaccaga tgaatctact ggatttgttc
    19921 ccaaagagta tagctttgct aattacatca ccaatgcctt tgggcagttt gataactata
    19981 cgcccatgca gttggctcag tatgtagcaa ctattgcaaa tgatggtgtt cgtgtggctc
    20041 ctcgtattgt tgaaggtatt tatggaaata atgataaggg tggccttggg gaattaatcc
    20101 aagcaataga taccaaggaa atcaacaagg tcaatatttc tgaatcagat atggcaatct
    20161 tgcaccaagg attttaccaa gtatcgcatg gaactagtcc ccttacgaca ggacgggcgt
    20221 tttcagatgg cgccactgtt tctatcagtg gtaagaccgg tacaggtgaa agctatgtag
    20281 ctggtggtca agaagctaat aataccaatg ccgtggccta tgctccaaca gaaaatcctc
    20341 aaattgcagt tgcagtagtc tttcctcata ataccaattt aaccaaaaat gttgggccag
    20401 caattgctcg cgacattatc aatttatata accaacacca tccaatgaat tagaaaggaa
    20461 gccatgcttt atccaacacc tattgctaag ttgattgaca gttattccaa acttcctggt
    20521 attgggatta agacagcaac tcgactggcc ttttatacta ttggaatgcc ggatgacgac
    20581 gtcaatgaat ttgcaaaaaa tcttcttgca gccaagcgag aattgaccta ttgctctatt
    20641 tgtggacgat taacagatga agacccgtgc tctatctgta ccgatccgac tcgtgaccag
    20701 acaacgattt tagttctcga agatagtcgc gatgtggcag ccatggagaa tatccaagaa
    20761 taccatggac tctatcatgt cctgcatggc ctcatttctc ctatgaatgg tatcagtcca
    20821 gacgatatca atctcaagag tctcatgact cgtctgatgg atagtgaagt ttcagaagta
    20881 atcgtagcaa ccaatgctac agcagatggg gaagcgactt ccatgtatct ttcccgctta
    20941 ctcaagccag ctgggattaa ggtcactcgc ctggcacgag gcctcgctgt gggagcagat
    21001 atcgagtatg cggacgaagt gacactctta cgagcgattg aaaatcggac agagttgtaa
    21061 gtgtaggcaa atttacgaac tccattcatt tataaaaaat caaagaggct gaaaatcgtt
    21121 cctatcggcc tctttttgta tagtgtgatg agtagaatca ggttcaagtt ttaaaaaacc
    21181 aagcaaatat gatatactaa agagcgagta ttctagtaga attaggacaa ataatatgaa
    21241 acaaacgatt attcttttat atggtggacg gagtgcggaa cgcgaagtct ctgtcctttc
    21301 agctgagagt gtcatgcgtg cggtcaatta cgaccgtttc acagtcaaga ctttctttat
    21361 cagtcagtca ggtgactcta tcaaaacgca ggaatttagt catgctccgg ggcaagaaga
    21421 ccgtctcatg accaatgaaa ccattgattg ggataagaaa gttgcaccaa gtgctatcta
    21481 cgaagaaggt gcagtggtct ttccagtcct tcacgggcca atgggagaag atggctctgt
    21541 tcaaggattc ttggaagttt tgaaaatgcc ttacgttggt tgcaacattt tgtcatcaag
    21601 tcttgccatg gataaaatca cgactaagcg tgttctggaa tctgctggta ttgcccaagt
    21661 tccttatgtg gctatcgttg aaggcgatga tgtgactgct aaaatagctg aagtggaaga
    21721 aaaattggct tatccagtct tcactaagcc gtcaaacatg gggtctagtg tcggtatttc
    21781 taagtctgaa aaccaagaag aactccgtca agccttaaaa cttgccttcc gatatgacag
    21841 ccgtgtcttg gttgagcaag gagtgaatgc ccgtgaaatt gaggttggcc tcttgggtaa
    21901 ctacgatgtc aagagcacgc tacctggaga agttgtcaag gacgttgcct tttatgacta
    21961 cgatgccaag tatattgata acaaggttac tatggatatt cctgccaaaa tcagtgatga
    22021 tgtggtggct gtcatgcgtc aaaatgcaga aacagccttc cgtgccattg gtggccttgg
    22081 tctatctcgt tgcgatttct tctatacaga taagggagag atttttctca acgagctcaa
    22141 taccatgcca ggtttcaccc agtggtctat gtacccacta ctttgggaaa atatggggat
    22201 cagctaccca gaactaatcg agcgtttggt tgaccttgcc aaggaaagtt ttgacaagcg
    22261 cgaagcgcat ttgatataaa atgaaagaga gggtagaagc cagaaccatc actgcaaggt
    22321 gactagagtt ctcggacttc agcccttttt aaaggagtag aaatgaaatt aacaatccat
    22381 gaaattgccc aagttgttgg agccaaaaat gatatcagta tctttgagga cactcagtta
    22441 gaaaaagctg agtttgatag tcgtttgatt ggaactggag atttatttgt gccacttaaa
    22501 ggtgcgcgtg atggccatga ctttattgaa acagcctttg aaaatggtgc agcagtaacc
    22561 ttgtctgaga aagaggtctc aaatcatcct tacattttag tagatgatgt tttgacagcc
    22621 tttcaatccc tagcatccta ctatcttgaa aaaacgactg ttgatgtctt tgctgttaca
    22681 ggttcaaatg gcaagacaac gactaaggat atgttggcgc atttactgtc aacaagatac
    22741 aagacctaca aaacacaagg caattacaat aatgagattg gccttcctta cacagttctt
    22801 catatgcctg aaggaacaga aaagttggtt ttggagatgg gacaggatca cttgggagat
    22861 attcatctct tgtctgaatt ggctcgtcca aaaacagcca tcgtgacctt ggttggagaa
    22921 gctcatttgg cctttttcaa agatcgttct gagattgcta aaggaaaaat gcaaattgca
    22981 gacggaatgg cttcaggttc cttgctttta gcgccggctg accctatcgt agaggactat
    23041 ttgccaattg ataaaaaggt ggttcgtttt ggtcaaggag cagagctgga aattactgac
    23101 ttggttgagc gtaaggacag tctgaccttt aaggctaatt tcttggaaca agcccttgat
    23161 ttgccagtaa ctggcaagta caatgcgaca aatgctatga ttgcatccta tgttgccttg
    23221 caagaaggag tttcagagga gcaaattcgt ttggcattcc aacatcttga attgacgcgt
    23281 aaccgtaccg agtggaagaa agcagccaat ggagcagata tcctgtcaga tgtttacaat
    23341 gccaatccaa ctgctatgaa actgatttta gagactttct ctgccattcc agccaatgaa
    23401 ggtggcaaga aaattgcagt gttggcggat atgaaggaac tgggtgacca gtctgttcaa
    23461 cttcataatc agatgatttt gagcctctcc ccagatgtgc ttgatatcgt tattttctat
    23521 ggagaagata ttgctcagtt ggctcaattg gccagtcaaa tgtttccaat cggccacgtt
    23581 tactacttca agaaaacaga agaccaggat caatttgaag acctagtcaa gcaggttaag
    23641 gaaagccttg gagcctatga ccaaatcttg ctaaaaggtt ctaactctat gaatctagcc
    23701 aaattggtag aaagtttaga aaatgaatgc aagtgatttt gtcaagtatc ttcaaagaat
    23761 gattgctctt acagatactg gactaacctt tacaaaggat ccgtttgacc gtgagcgcta
    23821 cgaagacttg cgaagtctgt tatctgaaat gttgaatcag gtatcagacc ttgatgcaga
    23881 cgaagtggca gaactcttga aaccgacctc cgcttatgcg actcctttaa tggacgtccg
    23941 tgcttggata gttgaggatg aaaaaatttg tctggttagg ggacaaggag agaatgattg
    24001 ggctttgcca ggtggctttg gcgaggtcgg ttattcacca actgaaaata ttctcaagga
    24061 aattgaagaa gaaaccggtt ttaaagccaa agttgaacga ctactagctg tttttgatac
    24121 caatcgtttc caactacaga gcaaacaata tgcaaagttt gtttttgaat gtaagcttct
    24181 cgatggacaa tttcaagaaa atcaagaaat cgctgacctt caattttttg caattgacca
    24241 actgccgaat ttatctgaaa aacgcattac caaggagcaa atagagcttc tttggcaggt
    24301 ttatcaaggt catagggggc aatatcttga ctaagaagat gattatcgta tttctaaatc
    24361 catttttaac aactagcatg gtataataat atgcaggaaa attttgaatt atgaggaaga
    24421 ctagatgaat ttatgggata ttttctttac gactcaggca accgagccgc ccaaatttga
    24481 ccttttttgg tatgttagcc tatttacgct cttagcctta accttttata cagcccatcg
    24541 ctatcgtgaa aataaggttt accaacgatt tttccaaatc ttgcagactg ttcagttaat
    24601 ccttctttat ggttggtact gggtcaatca tatgccactg tcagaaagcc taccctttta
    24661 ccattgccgt atggctatgt ttgtggtact cttgcttcct ggtcaatcca aatataaaca
    24721 atactttgca ttattgggaa catttgggac attagcagcc tttgtttatc cagtgccaga
    24781 tgcttaccct tttccacata tcaccattct atcctttatc tttggtcatt tagcactctt
    24841 ggggaactct ctagtttatc tattgagaca gtataatgcg cgattgctgg atgtgaaggg
    24901 aatttttctc atgacctttg ccctaaatgc cttgattttt gtggtcaatt tggtgacagg
    24961 tggcgattac ggatttttga caaaaccgcc attggttggg gatcacggtc tagtagctaa
    25021 ttatttactt gtttcaattg tgctggtagc tactatcagt ttgactaaga aaatcttaga
    25081 attcttttta gctcaagaag cagaaaaaat gattgcaaag gaagcttaac acagagcttt
    25141 cttttttgct cttagagagt ttttacaagc agcttataaa ataagaattt ctgaatagac
    25201 aaactcaaaa aatggctggg aaatttagga aaaaagcaag cacgattaaa ttttttgtgt
    25261 tataatattt tgtgaatagc tatgcctatg tttagctatg gaataatacg aagtgcgaaa
    25321 cttggaagat agagaggaag cgatgtaatg gctagagaag gcttttttac aggtctagat
    25381 attggaacaa gctctgtcaa ggtgcttgtg gccgagcaga gaaatggtga attaaatgta
    25441 attggcgtga gtaatgccaa aagtaaaggt gtaaaggatg gaattattgt tgatattgat
    25501 gcagcagcaa ctgctatcaa gtcagccatt tcccaagcgg aagaaaaggc aggcatttcg
    25561 attaaatcag tgaatgtcgg cttgcctggt aatcttttgc aggtagaacc aactcagggg
    25621 atgattccag taacatctga tactaaggaa attacggatc aagatgttga aaatgttgtc
    25681 aaatcagctt tgacaaagag tatgacacct gaccgtgaag tcattacctt tattcctgaa
    25741 gaatttattg tggatggttt ccaagggatt cgtgacccac gtggcatgat gggggttcgc
    25801 cttgaaatgc gtggtttgct ttatacagga cctcgtacta tcttgcacaa tttgcgtaag
    25861 acggttgagc gtgcaggtgt tcaggttgaa aatgttatca tttcaccact agcaatggtt
    25921 cagtctgttt tgaacgaagg ggaacgtgaa tttggtgcta cagtgattga tatgggggca
    25981 ggtcaaacga ctgtcgctac aatccgtaat caagaactcc agttcacaca tattctccaa
    26041 gaaggtggag attatgtaac taaagatatc tccaaggttt tgaaaacctc tcgcaaatta
    26101 gcggaaggct taaaactgaa ttacggggaa gcctatccgc ctcttgcaag caaagaaacc
    26161 ttccaagtag aggttattgg agaagtagaa gcagtcgaag tgacggaagc ctacttgtca
    26221 gaaattattt ctgcacgaat caagcacatc cttgaacaaa tcaagcaaga attagataga
    26281 aggcgtctat tggacctccc tggtggtatt gtcttaatcg gtgggaatgc cattttacca
    26341 ggtatggttg agcttgctca ggaagtcttt ggcgtccgtg tcaagcttta tgttccaaat
    26401 caagttggta tccgtaatcc agcctttgcg catgtgatta gtttatcaga atttgcgggt
    26461 caattaacag aagttaatct tttggctcag ggagcgataa aaggtgagaa tgacttaagt
    26521 catcagccaa ttagttttgg tgggatgctg caaaaaacag ctcagtttgt acaatcaacg
    26581 cctgttcaac cagctcctgc tccagaagta gagccggtgg cgcctacaga accaatggcg
    26641 gatttccaac aagcttcaca aaataaaccg aaattagcag atcgtttccg tggcttgatc
    26701 ggaagcatgt ttgacgaata aagaggaaaa ataaattatg acattttcat ttgatacagc
    26761 tgctgctcaa ggggcagtga ttaaagtaat tggtgtcggt ggaggtggtg gcaatgccat
    26821 caaccgtatg gtcgacgaag gtgttacagg cgtagaattt atcgcagcaa acacagatgt
    26881 acaagcattg agtagtacaa aagctgagac tgttattcag ttgggaccta aattgactcg
    26941 tggtttgggt gcaggaggtc aacctgaggt tggtcgtaaa gccgctgaag aaagcgaaga
    27001 aacactgacg gaagctatta gtggtgccga tatggtcttc atcactgctg gtatgggagg
    27061 aggctctgga actggagctg ctcctgttat tgctcgtatc gccaaagatt taggtgcgct
    27121 tacagttggt gttgtaacac gtccctttgg ttttgaagga agtaagcgtg gacaatttgc
    27181 tgtagaagga atcaatcaac ttcgtgagca tgtagacact ctattgatta tctcaaacaa
    27241 caatttgctt gaaattgttg ataagaaaac accgcttttg gaggctctta gcgaagcgga
    27301 taacgttctt cgtcaaggtg ttcaagggat taccgatttg attaccaatc caggattgat
    27361 taaccttgac tttgccgatg tgaaaacggt aatggcaaac aaagggaatg ctcttatggg
    27421 tattggtatc ggtagtggag aagaacgtgt ggtagaagcg gcacgtaagg caatctattc
    27481 accacttctt gaaacaacta ttgacggtgc tgaggatgtt atcgtcaacg ttactggtgg
    27541 tcttgactta accttgattg aggcagaaga ggcttcacaa attgtgaacc aggcagcagg
    27601 tcaaggagtg aacatctggc tcggtacttc aattgatgaa agtatgcgtg atgaaattcg
    27661 tgtaacagtt gtcgcaacgg gtgttcgtca agaccgcgta gaaaaggttg tggctccaca
    27721 agctagatct gctactaact accgtgagac agtgaaacca gctcattcac atggctttga
    27781 tcgtcatttt gatatggcag aaacagttga attgccaaaa caaaatccac gtcgtttgga
    27841 accaactcag gcatctgctt ttggtgattg ggatcttcgc cgtgaatcga ttgttcgtac
    27901 aacagattca gtcgtttctc cagtcgagcg ctttgaagcc ccaatttcac aagatgaaga
    27961 tgaattggat acacctccat ttttcaaaaa tcgttaagta aatgaatgta aaagaaaata
    28021 cagaacttgt ttttcgagaa gttgcagagg ctagtctgag tgctcatcga gagagtggtt
    28081 cggtctctgt cattgcagtt accaagtatg tagatgtacc gacagcggaa gccttgcttc
    28141 cgctaggtgt tcatcatatc ggtgaaaatc gtgtagataa gtttctggaa aaatatgaag
    28201 ctttaaaaga tcgagatgtg acttggcatt tgattggtac cttgcaaaga cgtaaggtga
    28261 aagatgtcat tcaatacgtt gattatttcc atgcattgga ctcagtaaag ctagcagggg
    28321 aaattcaaaa aagaagtgac cgagtcatca agtgtttcct tcaagtaaat atttctaaag
    28381 aagaaagcaa acacggtttt tcgagagagg aactgctgga aatcttgcca gagttagcca
    28441 gactagataa gattgaatat gttggtttaa tgacgatggc accttttgag gctagcagtg
    28501 agcagttgaa agagattttc aaggcggccc aagatttaca aagagaaatt caagagaaac
    28561 aaattccaaa tatgcctatg accgagttaa gtatgggaat gagtcgtgat tataaagaag
    28621 cgattcaatt cggttccact tttgttcgta taggtacatc attttttaag taggagagaa
    28681 ccatgtcttt aaaagataga ttcgatagat ttatagatta ttttacggag gatgaggatt
    28741 caagtctccc ttatgaaaaa agagatgagc ctgtgtttac tccagtaaat tcttcacagg
    28801 aaccggctct cccaatgaat caaccttcac agtcggctgg tacaaaagag aacaatatca
    28861 ccagacttca tgcaagacaa caggaattgg caaatcagag tcagcgtgca acggataagg
    28921 tcattataga tgttcgttat cctagaaaat atgaggatgc aacagaaatt gttgatttat
    28981 tggcaggaaa cgaaagtatc ttgattgatt ttcagtatat gacagaggtg caggctcgtc
    29041 gttgtttgga ctatttggat ggagcttgtc atgttttagc tggaaatttg aaaaaggtag
    29101 cttctaccat gtatttgttg acaccagtta atgttattgt aaatgttgaa gatatccgtt
    29161 taccagatga agatcaacag ggtgagttcg gttttgatat gaagcgaaat agagtacgat
    29221 aatgattttt ttaattcgta tgatttataa tgcagtggat atttactccc tgattttggt
    29281 agccttcgct gtcatgtctt ggtttccagg tgcctacgaa tccagtttag gtcgttggat
    29341 tgtagcgttg gtgaaaccag tacttgctcc cttgcaacgc ctgcctttac agatagcggg
    29401 tcttgattta tctgtttggg tcgcgattgt tttggttcga tttttaggag aaaacctagt
    29461 gcgttttctg gcgatgatag gatgaataaa gggatttatc agcatttctc catagaagat
    29521 cgtccatttc ttgacaaggg aatggaatgg ataaagaagg tagaagatag ctatgctcct
    29581 tttttaactc cttttatcaa tcctcatcag gagaagctat taaagatttt ggccaaaacc
    29641 tatggtcttg cttgtagcag tagtggggaa ttcgtctcga gtgagtatgt tcgagtttta
    29701 ttatacccag attatttcca accagagttt tcagattttg aaatatctct ccaagaaatt
    29761 gtgtattcca ataaatttga acatttaacg catgctaaga ttttagggac agtcatcaat
    29821 caattaggga ttgaacggaa actttttgga gatatcctag tagatgaaga acgggcgcag
    29881 attatgatta atcagcagtt tcttcttctc tttcaagatg gactaaagaa aattggtcgt
    29941 atacctgttt cgctggagga acgtcctttc accgagaaaa tagataagct agaacagtat
    30001 cgagaactgg atttatctgt gtctagtttt cgattagatg ttcttttatc aaatgttttg
    30061 aaactatcta ggaatcaagc aaaccagttg attgaaaaga aacttgtcca agtaaattat
    30121 catgtggtag acaaatcaga ttacactgtt caagttggag acttgattag tgtgagaaaa
    30181 tttggtcgct tgagattact tcaagataag ggacaaacga aaaaagagaa gaaaaaaata
    30241 accgtccagt tattattaag taagtgagga atagaatgcc aattacatca ttagaaataa
    30301 aggacaagac ttttggaact cgattcagag gttttgatcc agaagaagtc gatgaatttt
    30361 tagatattgt ggttcgtgat tacgaagatc ttgtgcgtgc gaatcatgat aaaaatttgc
    30421 gtattaagag tttagaagag cgtttgtctt actttgatga aataaaagat tcattgagcc
    30481 agtctgtatt gattgctcag gatacagctg agagagtgaa acaggcggcg catgaacgtt
    30541 caaacaatat cattcatcaa gcagagcaag atgcgcaacg cttgttggaa gaagctaaat
    30601 ataaggcaaa cgagattctt cgtcaagcaa ctgataatgc taagaaagtc gctgttgaaa
    30661 cagaagaatt gaagaacaag agccgtgtct tccaccaacg tctcaaatct acaattgaga
    30721 gtcagttggc tattgttgaa tcttcagatt gggaagatat tctccgtcca acagctactt
    30781 atcttcaaac tagtgatgaa gcctttaaag aagtggttag cgaagtactt ggagaaccga
    30841 ttccagctcc aattgaagaa gaaccaattg atatgacacg tcagttctct caagcagaaa
    30901 tggaagaatt acaagctcgt attgaggtag ccgataaaga attgtctgaa tttgaagctc
    30961 agattaaaca ggaagtggaa actccaactc ctgtagtgag tcctcaagtt gaagaagagc
    31021 ctctgctcat ccagttggcc caatgtatga agaaccagaa gtagctccag tgcatccgac
    31081 aggtccaaca ccagctacag aaactgttga ttcagcacca ggatttgaag caccgcaaga
    31141 atccgttaca attttataag aaacgatgtg agaacaatat cttatcctta tatttccagc
    31201 gagcaggaga tggtgtgagt cctgcattcc ctaacgataa gattatcctc tcaaaaactc
    31261 aagtctgaag ctagtaagat ttgacgtttc ccacgttacg ggataagagg gagaaagact
    31321 aaatcttttt ccgaataaag gtggtaccac gattttcgtc ctttttggaa gtcgtggttt
    31381 ttaatttgtt attatttata aaggagatac catgaaactc aaagataccc ttaatcttgg
    31441 gaaaacagct ttcccaatgc gtgcaggcct tccgaccaaa gagccagttt ggcaaaagga
    31501 atgggaagat gcaaaacttt accaacgtcg tcaagaacta aatgaaggga aaccgcattt
    31561 tgtcttgcat gatggccctc catacgcaaa cggaaatatc cacgttggac atgccatgaa
    31621 tcatatctca aaagatatca tcattcgttc taagtctatg tcaggtttta acgcacctta
    31681 tatcccaggt tgggatactc atggtctgcc aatcgagcaa gttttggcaa agcaaggtgt
    31741 gaagcgcaaa gaaatggact tggttgagta cttgaaactc tgtcgtgagt acgctctttc
    31801 tcaagtttat aaacaacgtg atgatttcaa acgcttgggg atgtcaggtg actgggaaaa
    31861 tttatatgtg actttaactc cagactatga agcagcccaa atccgtgtct ttggtgagat
    31921 ggctaataag ggttatatct accgtggtgc caagccagtt tactggtcat ggtcatctga
    31981 gtcagccctt gccgaagcgg aaattgaata ccatgatttg gtttcgactt cactttatta
    32041 tgccaataag gtaaaagatg gcaaaggtat cctagataca gatacttata tcgtagtctg
    32101 gacaacaact ccatttacca tcacagcttc tcgtggtttg actgttggtg cagatattga
    32161 ttacgttttg gttcaacctg ctagtgaaac tcgtaagttt gtcgttgctg cagaattgtt
    32221 gactagcttg tctgagaaat ttggctgggc tgatgttcaa gtgttggcaa cttaccgtgg
    32281 tcaagaactc aaccacatcg taacagaaca cccatgggat acagctgtag atgagttggt
    32341 aatccttggt gatcacgtta ccactgactc tggtacaggt atcgtccata cagcccctgg
    32401 ttttggtgag gacgactaca atgttggtat tgctaatggt cttgaagtcg cagtgactgt
    32461 tgacgaacgc ggtatcatga tggcgaatgc tggtcctgag tttgaaggtc agttctacga
    32521 caaggttgta ccaactgtta ttgaaaaact tggtaacctc cttctagccc aagaagaaat
    32581 ctctcactca tacccatttg actggcgtac taagaaacca atcatctggc gtgcagttcc
    32641 acaatggttt gcctcagttt caaaattccg ccaagaaatc ttggatgcta ttgataaagt
    32701 taaattccat acagaatggg gtaaagtacg tctatataat atgattcgtg accgtggtga
    32761 ctgggttatc tctcgtcaac gtgcttgggg tgttccactt ccaatcttct atgcagaaga
    32821 cggtacagct atcatgacag ctgagactat tgaacatgtg gcgcaactct ttgaagtaca
    32881 tggttcaagc atttggtggg aacgtgatgc caaagacctc ttaccagaag gatttactca
    32941 tccaggttca ccaaacggcg agttcaagaa agaaacagat attatggatg tatggtttga
    33001 ctcaggttca tcatggaatg gtgttttggt aaatcgtcca aatctgactt atccagctga
    33061 cctttacctt gaaggttctg accaataccg tggttggttc aactcatcac tcattacatc
    33121 tgttgccaac catggcgtag caccttacaa acaaatcttg tcacaaggtt ttacccttga
    33181 tggtaaaggt gagaagatgt ctaaatcact tggaaatacc attgctccaa gtgatgttga
    33241 aaaacaattt ggtgcagaaa tcttgcgtct ctgggtaaca agtgttgact caagcaatga
    33301 cgtgcgtatc tctatggata ttttgagcca agtttctgaa acttaccgta agattcgtaa
    33361 cactcttcgt ttcttgattg ccaatacatc tgactttaac ccagctcaag atgtagtcgc
    33421 ttacgatgaa cttcgttcag ttgataagta catgacgatt cgctttaacc agcttgtcaa
    33481 gaccattcgt gatgcctatg cggactttga attcttgaca atctacaagg ccttggtgaa
    33541 ctttatcaac gttgacttgt cagccttcta ccttgatttt gccaaagatg ttgtttacat
    33601 tgaaggtgcc aaatcattgg aacgccgtca aatgcagact gttttctatg acattcttgt
    33661 caaaatcacc aaactcttga caccaatcct tcctcacact gcggaagaaa tctggtcata
    33721 tcttgagttt gaagctgaag acttcgttca attgtcagaa ttgccagaag ctcaaacttt
    33781 tgctaatcaa gaagaagtct tggatacatg ggcagccttc atggacttcc gtggacaagc
    33841 tcaaaaagcc ttggaagaag ctcgtaatgc aaaagttatc gggaaatcgc ttgaagcaca
    33901 cttgacagtt tatccaaatg aagttgtgaa aactctactc gaagcagtaa acagcaatgt
    33961 ggctcaactt ttgatcgtgt cagacttgac catcgcagaa gggccagctc cagaagctgc
    34021 ccttagcttc gaagatgtag ccttcacagt tgaacgcgct gcaggtcaag tatgtgaccg
    34081 ttgccgtcgt atcgacccaa caacagcaga acgcagctac caggcagtca tctgtgacca
    34141 ctgtgcaagc atcgtagaag aaaattttgc ggaagcagtc gcagaaggat ttgaagagaa
    34201 ataaggttga aaagtctagg caaaattcaa tttgagaaga aaagacaact aattttatag
    34261 tctattaaac gcattgtatc acgtttttga atacctgata tgatgcgttt tttatttatt
    34321 ttaaaaattt gcgaggtatg actttttata ctcaacaaga atcaaagaga aacttagcaa
    34381 gctaacagta gtaagataaa ataggaattt gatattaggg ataagattgc taaatagtgt
    34441 aatattttta caataataaa tttatatagt tatttttggt ttctgaaaag tattatattt
    34501 tatttcatat tatacaaatt tttattttat aatatcagaa aatacttttt ttaaaagcaa
    34561 atatgataca attttatttg aaaaaaataa aaaaggagat tttattatga aattaaaaac
    34621 acttgcttta attagtggta tcgtcggtct tgtgggagga attttacttc ttattggtcc
    34681 ttttgtcttg ttgggaacag cggtaaacac agttgctaca actcttaatg gaggagctac
    34741 tgcaggggct ttttcaggtg ttgccttact cttgaatgcc ttgaagattg caaatcttgt
    34801 tcttggtatc attgctattg tttactataa aggagataat cgtgtaggtg cagctccgtc
    34861 tgtactaatg attgtttttg gtggagttag tctcattcca ttcttaggat gggttggggg
    34921 gattcttgtt attatcggag gatctctatt ccttgcaaca ttgaagaaat tcaaatcaga
    34981 agaataaaag gtattttagc atgaaaagaa caaaaaagtt tatcggtata ggagtagctc
    35041 tattatctct ttttcttcta gttgcatgtg gaacataaag ttcaaaggat acttcaacaa
    35101 gtaatgatga gaagacagta gcaacatcca atagttcaaa agaaacaatc actttcgata
    35161 caccggttgt aacagacgat gtgattgaat caatacgcac ttatgcagat tatatagatc
    35221 tttataaaaa tatttttgat gattatttta ctaaagctga ggaaggtttc aaaggcacag
    35281 ctatggaaaa taatgactcg tttactaaac taaaagagtc aactcaaaaa ttattcgatg
    35341 cgcagaaaaa aaggttaaat aatgaagata gaatagaaac aactaaaaac aatgtgattg
    35401 ccaaacattg tcaaacagtc ctttcctttt tggttttgac tagctttttt gtgaaaaatt
    35461 gtgtaaaata gaatagataa acgaggggaa acctcggaaa atttaaagga gaatccatct
    35521 aatggtaaaa ttggtttttg ctcgccacgg tgagtctgaa tggaacaaag ctaacctttt
    35581 cactggttgg gctgatgttg atttgtctga aaaaggtaca caacaagcga ttgacgctgg
    35641 taaattgatc aaagaagctg gtatcaaatt tgaccaagct tacacttcag tattgaaacg
    35701 tgctatcaaa acaactaact tggctcttga agcttctgac caattgtggg ttccagttga
    35761 aaaatcatgg cgcttgaacg aacgtcacta cggtggtttg actggtaaaa ataaagctga
    35821 agctgctgaa caatttggtg atgagcaagt tcacatctgg cgtcgttcat acgatgtatt
    35881 gcctccaaat atggaccgtg atgatgagca ctcagctcac acagaccgtc gttatgcttc
    35941 acttgacgac tcagtaatcc cagatgctga aaacttgaaa gtgactttgg aacgtgctct
    36001 tccattctgg gaagataaaa tcgctccagc tcttaaagat ggtaaaaacg tattcgtagg
    36061 agctcacggt aactcaatcc gtgcccttgt aaaacacatc aaaggtttgt cagatgacga
    36121 gatcatggac gtggaaatcc ctaacttccc accattggta ttcgaattcg acgaaaaatt
    36181 gaacgtcgtt tctgaatact accttggaaa ataaaaaatt gtaagtctag aattgatttc
    36241 taggcttttt atgttagtat ggaagtatga taaggaataa aaaacaagat tatgtactgg
    36301 cctacaagca accagtttca accacttaca tgggttggga agaagaagct ttaccgatag
    36361 gcaatggttc tttaggagca aaagtatttg gccttatagg ggctgaacgg attcaattta
    36421 atgaaaaaag tctctggtct ggaggtccac ttcctgatag ttcagattat cagggtggaa
    36481 atcttcagga tcagtatgtt tttttagctg agattcggca ggctttggag aagagagatt
    36541 acaatctggc taaggaactg gctgagcagc acctaattgg gccaaaaacg agtcaatatg
    36601 ggacctatct gtcttttggg gatattcaca ttgagttcag ccagcaaggt acgactttgt
    36661 ctcaggtgac ggactatcag agacagctga atattagtaa ggcacttgtg acgacttctt
    36721 atgtctataa gggaacgcga tttgaacgtg aagcttttgc gagttttcca gatgatctct
    36781 tggttcagcg ttttactaag gaaggggcgg aaactctaga ttttactata gaactatcct
    36841 tgacctgtga tttggcttct gatggaaagt atgagcagga aaaatctgat tacaaggagt
    36901 gtaagttgga tattactgat tctcatatct tgatgaaggg aagagttaag gataatgatc
    36961 tgcggtttgc tagttatcta gcttgggaaa cggatggaga tattagagtt tggtcagata
    37021 gggttcagat atcaggagcc agttatgcca atctcttctt ggccgctaag acggattttg
    37081 cccaaaatcc tgctagcaat tatcgcaaga aactagattt agagcaacag gtgatagact
    37141 tggtggacac agctaaagaa aagggctata cccaattgaa atcaaggcat atcgaggact
    37201 accaagcctt attccagcgt gttcaattgg atttggaagc taatgttgac gcattcacta
    37261 cagatgattt gttaaaaaat tataagccac aagaagggca ggctttggag gagctgttct
    37321 tccagtatgg acggtattta ttgattagtt cgtccagaga ctgcccagat gctctaccag
    37381 ctaacctaca gggagtctgg aatgcggtcg acaatcctcc ttggaattcg gactatcact
    37441 taaatgtcaa tctgcagatg aattattggc cagcctatgt taccaatctc ctagaggcgg
    37501 tctttccggt catcaactat gtagatgatt tgcgtgtcta tggtcgtcta gcggctgtaa
    37561 agtatgcagg aatcgtctct caaaaaggtg aggaaaatgg ttggttggtt catactcaag
    37621 cgactccctt tggttggacg gcacctggtt gggattacta ttggggttgg tcaccagctg
    37681 ccaatgcgtg gatgatgcaa actgtttatg aagcctattc attttatagg gaccaagact
    37741 atctcaggga gaaaatttat cccatgttga gggaaacggt tcgtttttgg aatgcctttt
    37801 tacataagga tcagcaggcg cagcgttggg tgtcttctcc gtcttattcc ccagaacatg
    37861 ggccgatttc gattggcaat acttatgacc aatctctgat ttggcagtta tttcatgatt
    37921 ttattcaggc tgctcaggaa ttgggactgg atgaggactt gttgactgag gttaaggaga
    37981 agtctgattt actaaatcct ttgcaaatca ctcaatctgg tcgaatcagg gagtggtatg
    38041 aggaggaaga gcagtatttt caaaatgaga aagtggaggc ccagcaccgg cacgcttccc
    38101 atctagtggg actctatcct ggcaatctct ttagctacaa gggacaagag tatattgaag
    38161 cggcgcgtgc tagcctcaat gatcgtggag atggcggcac aggctggtcc aaggctaata
    38221 agatcaatct ctgggcgcgt ttgggagatg gcaatcgagc ccataaatta ttggcagagc
    38281 agttaaagac atccaccttg ccaaatcttt ggtgtagcca tcctcctttt cagatagatg
    38341 gtaattttgg tgctactagt ggcatggcag aaatgttact ccagtctcat gcagcttatc
    38401 tggtacctct agctgcccta cctgatgctt ggtcaacagg ttctgtttca ggcttaatgg
    38461 cacgtggaca ttttgaagtg agcatgagct gggaagataa aaaactctta cagttgacca
    38521 ttttatcaag gagtggagga gatttgcgag tttcttatcc agatattgag aagagtgtga
    38581 ttaaaatgaa tcaagaaaaa ataaaagcga aatgcatggg gaaagattgt atttcggtgg
    38641 caacagcaga aggtgatctt gttcaatttt atttttaaga agatgttata aggcagtaat
    38701 ttgaaactgc cttttaataa ggatttaaga atataagcag ttttcaacta gttgaaaaag
    38761 cgttataatg ataataggaa gtaatactca atgaaaatca aagagcacaa acaaggaagc
    38821 tagccgcagg ttgctcaaaa cagtgttttg aggttgcaga tggaagctga cgtggtttga
    38881 agagagattt tcgaggagta taatttgttt gatagagggt gggtctgatg gcttatattg
    38941 agatgaaaca ctgttacaag cgttatcagg ttggggacac ggagattgtg gccaatcgtg
    39001 atgtgaattt tgagattgaa aagggggagc tggttattat ccttggtgct tcaggtgcag
    39061 gcaagtcaac agtgcttaac cttcttgggg gaatggatac caatgatgaa ggggaaatct
    39121 ggattgatgg tgttaatatt gcggattata gttcccacca gcgcaccaat taccgtagaa
    39181 atgatgtggg gtttgttttt cagttttata atctagtttc taatctgaca gctaaggaaa
    39241 atgtggaact ggcttctgaa attgtgacag atgccttgaa tcctgatcag gtcttgacag
    39301 atgtaggtct ggctcatcgt ctcaataact ttccagccca gctttctgga ggggagcaac
    39361 agcgagtctc cattgcacgc gcggtagcca aaaatcctaa aattctcctt tgtgatgaac
    39421 cgactggagc cttggattat cagacgggca agcaggtttt gaaaattctc caagacatgt
    39481 ctcgtcaaaa gggagcgacg gtgatcatcg tgactcataa tggagctttg gcgcccattg
    39541 ctgatcgcgt gattcatatg cacgatgcca gtgtcaagga tgtggtgctc aaccagcatc
    39601 ctcaggatat tgacagtttg gagtactagc atgatcaagc gaaaaactta ttggaaggac
    39661 ttaattcagt ccttcacaga ctccaagggg cgttttttat ccatcttgat cctgatgatg
    39721 ttgggatctc tagccttagt aggcctcaaa gtaaccagtc ccaacatgga ggcgacagct
    39781 aatgcttatt taacaactgc tcaaaccttg gatttggcag tcatgtctaa ctatggcttg
    39841 gatcaagcag accaagaaga actaaaacag acggagggcg cagaggtcga gtttggctat
    39901 ttgacagatg tgactatgga taatgggcag gatgccattc gactgtactc caaaccagag
    39961 cgaatttcaa cctttcagct aagaaaggga cgacttcctc agtcagacag ggaaatcgct
    40021 ttggccactc atttgcaagg ccaatacagc gtgggacagg agattagttt taaagaaaaa
    40081 gaagagggtc attcctcttt aaaagaccat acttatacca ttactggttt tgtggattcg
    40141 gctgaaatcc tctcccagcg agatatgggc tacgcaggaa gtggaagtgg gactctgaca
    40201 gcctatgggg tgattttacc tagtcaattt gatcagaaag tctacaatat agctcgtttg
    40261 aaatatcaag atttagcagg tttaaatgcc ttttcatcag cttatgaaga aaaatccaag
    40321 caacatcagg aagaccttga acaaacttta tcagataatg gcaaggtacg tctgcaactt
    40381 ttgaaaaaag aaggacaaga gtctctagac aaggggcaag agactcttga caaggctcag
    40441 actaatttgc aggaaggcaa gcgtcgttta gcagctgctc aagctcgtat acaggctcaa
    40501 gaaagtcaac tagccttgtt tcctcaagct cagagagagc aggctagtgc tcaacttacc
    40561 caagccaagc aggaactggg caaggaagag gacaaactaa agcaagctga acaaaatcta
    40621 gcccaagaaa aggaaaaatt agaaaaacat cagcaagtct tggatgattt ggcggagcca
    40681 aggtatcagg tttataatcg tcagaccatg ccaggtggtc agggctatct tatgtatagc
    40741 aatgcttcat ccagtattcg agcagtgggc aatatctttc ctgtggtact ttatgccgta
    40801 gcagccatgg tgacctttac gaccatgact cgctttgtag acgaagagcg aactcatgca
    40861 gggattttta aggccttggg ttatcgtagt aaggatatta tcgccaagtt tctcctttat
    40921 ggactagtag ctgggactgt cggaacggct ctaggtagta tacttggtca ttatttgcta
    40981 gccagtgtaa tttcaagtgt cattacaaaa ggcatggtgg tgggagaaac ccagattcag
    41041 ttctattgga cctatagctt actagctctt gtcttgagct ggttggcgag tgtgttacca
    41101 gcctatctgg tggcttggag ggaacttcat gacgaagcag cccagcttct acttcctaaa
    41161 cctcctgtca aaggagctaa aatcttattg gagcgtatcg gttttatctg gcgtcgtctc
    41221 agttttactc ataaggtaac agcccgcaac atctttcgtt ataagcagag aatgttgatg
    41281 acaatctttg gtgtggcagg ttctgtagct ctgctctttg caggtttggg aatccaatct
    41341 tctgtagcag gagttccgtc taaacagttt caacaaatcc aacagtatca gatgcttgtc
    41401 tctgaaaatc ctagtgcgac caatcaggac aaggtagagc tagcagaagt gttgaaaggg
    41461 caggagatac tagcctacca gaaaatctat tctaaaacgc tagacaagga tttcaaaggc
    41521 aaggctggtc ttcaaaacat tactcttatg atgatagaga aggaagattt gactcccttt
    41581 atccatcttc aacatcatca gcaggagctg acattaaaag atggcatcgt tattacagct
    41641 aaactcgccc agctggcagg tgtcaaggtt gggcagactt tagaaattga aggtaaggaa
    41701 ctaaaggtcg ctgctattac tgagaactac gttggtcact ttatttatat gagtcaggct
    41761 agctatgagc aactttacgg acagctaccc caagccaaca cttatctggt ctcattaagg
    41821 gataccagtg caactagtat cgaaagtcag gcgggcttgc ttatgagtca atctgcggtg
    41881 tccagcgttg tccaaaatgc ttcagccatt cgactcttcg actctatcgc tagctcactc
    41941 aatcagacca tgaccatctt ggtcatcgta tcggttctat tagctattgt catcctttac
    42001 aatctgacca atatcaacgt agctgagaga atccgtgaac tctccactat caaggttctc
    42061 ggttttcata ataatgaagt caccctctac atttaccgtg agacgattgt gctgtccctt
    42121 gtgggaatcg tacttggtct ggtagctggt ttctatttac accaattttt gattcaaatg
    42181 atttcgcctg cgactattct cttttatccg caggtaggct gggaagtcta tgtaatccca
    42241 gtggcagcag taagcatcat tttgaccttg cttggtttct tcgtcaatta ttatctgaga
    42301 aaggttgaca tgttagaagc cctgaaatct gtagagtaag gtagttattt ttagctgatt
    42361 gaacttctat ttactaatat tcaaaaatcc tccgtttcaa agagcaggga actctttgtg
    42421 acagaggatt ttttctatag ggctttagca gctgcaattg cggcttcgaa gtttggctca
    42481 gaattgatat tatccacgta ttcaacgtag cgaatcgtat tgtcagtatc gaggacaaag
    42541 actgcgcgtg ctaataggtg ccattcgttg atcaagaggg cataatcgcg tccgaaagaa
    42601 tggtcaaagt agtctgaaag cataatggca ttgtcaaggc cttcagcacc gcaccaacgt
    42661 ttttgagcaa aaggtaggtc cattgaaaca gtcaatacga ccgtgttgtc cagtccagcc
    42721 aattcttcat taaaacgacg tgtttgagtt gagcagatgc ctgtatcgat agaaggaacg
    42781 acactcaaga cttttttctt gccatcaaaa tcagccagag attttttaga aagatctgtt
    42841 gtagtaagag aaaaatcaag cgccttgtcg ccgacttgta gttgtttacc tgtaaagctc
    42901 acaggatttc cgagaaaagt taccatagga tactccaatc ttttttcttc cattgtatct
    42961 gaaacagtca gaattttcca atgatttgac cggaaatgtg tgcatagaaa aaacgccagc
    43021 tcacatgaga atgacgtttt tcagaggctt attttgccaa tccttcagca atcttgtcaa
    43081 ggttgtattt catcatgctg tagtagctgt cgccttcttt accttgttct gcgatagagt
    43141 cagtaaagat ttgtgcgtag attgggatgt ttgtgtcttg agaaacagtt ttcattggac
    43201 ggtcatccac acttgattct acaaagagtg atggaacttt tgtttggcga agtttttcaa
    43261 ccaaggtctt gatttgttca ggagttcctt cttcttcagt attgatttcc cagatgtagg
    43321 cacttgggac accataggct ttagagaagt atttgaatgc tccttcgctg gttacaatga
    43381 gtttcttttc agcagggatc ttattaaatt tatccttact ttctttatca agtttgtcta
    43441 acttatcagt atattctttg agattttttt catagaattc tttattgtta gggtctttgg
    43501 cgctcaattg tttggcgata tttttagcaa aaataatacc gttttcaagg ttaagccaag
    43561 cgtgtgggtc ttcttttcct ttttcatttt gaccttcaag gtagataaca tcaacgccgt
    43621 cgctgactgc gaagtagtct ttgttttcag ttttcttggc attttctacc aattttgtaa
    43681 accaagcatt gccacctgtt tcaaggttga taccgttata gaaaatcaaa tcagcctcag
    43741 aagttttctt aacgtcttca ggaagtggtt cgtattcgtg tgggtcttgc ccaatcggaa
    43801 cgatactatg aaggtcaatt ttgtcaccag caatattttt agtaatatca gcgatgattg
    43861 agtttgtagc aacaactttt agtttttgac cagaagttgt atcttttttt ccgctagcac
    43921 atgctacaag aatgattgca gaaagaaaga gaacgagtaa tgtacctaat tttttcatta
    43981 gatcctccaa tttattaggg ctttgcccct tattttaaca aatgtttatt tttcagtttc
    44041 aaatatcgtt gtttgggagc gataaagaag ctaatgagaa agaaactagc agctgtaagc
    44101 acgatactag aacctgccgc aacattaaaa ctatagccaa taaagagtcc caaaactgaa
    44161 gcagtagctc cgaaggttga ggaaaggaaa atcatgcttt tcagactatt agcatacaga
    44221 taagcagttg cagctggggt aatcagcatg gctacaatca ggatagttcc gacactttgc
    44281 atggctgtca cagacacgag agtcaggagt accatgagaa ggtagtgata gaaattgaca
    44341 ggcattccca tggctttagc caagagttca tcaaaggaag ttatcaagag ttgcttgaag
    44401 aaaatccaga ttaacaagag gatggctgcc cccacaccca tagtaataaa catatccgta
    44461 tcttggacgg ccagaatatt accaaaaagg atatggaaaa ggtcagttga acttttagcg
    44521 acaccaatca agatgatacc gagggctaag aaagaagaaa aggtaatgcc gatggcggta
    44581 tcgcttttga taatcgagtt tcctttgatg taggtaatga tgatggcagc tagcaatcca
    44641 aagacaatgg ctccgataaa gaagtcaagg cccaagatga aggagagggc tacacctggt
    44701 aagacagcat gtgaaatggc atctcccatg agtgacatcc cgcgtagaat aatgaaacat
    44761 cccacggctc cagctacaat cccgacgaca atagctgtta tcaaggcatt ttgtaggaaa
    44821 tggaattttt gcaatccatc gataaattct gcaatcatag gtcacctcca ttgaaaaaga
    44881 gttgattacc gtaagcttct tttagattgg tttcggtaaa agtttctttt gttggaccaa
    44941 aggcaatcac ttctcgattg acaagtaaga cttgatcgaa gtagtgggga atcttgctga
    45001 ggtcgtggtg aacgatgaga accgtcttcc cagctttttt caaatctctc agcgtattca
    45061 tgatgatttc ctcactgaca gagtcaatcc cagcaaaggg ttcatccaag aggatatagt
    45121 cggcttcctg caccaaacat ctggcaatca agacccgctg gaattgacct ccagacagtt
    45181 gactaatttg acgttcagcg tagtcagcta ggccgacgat ttcaagggcc tcttgcactt
    45241 tcttccaatg tttagccttt aaacttcgaa agagaggaat agagggaaat agtcctaacg
    45301 agacgcattc cttgaccttg atgggaaagt tgtagtcgat attgattttt tgttcgacat
    45361 aggcaattcg gtgtaaggat tttttaactt ccttgtcatc gagaaatgcc tgaccttgat
    45421 gtgggataat tcccagcata ccttttaata gtgttgattt cccagcgccg tttggaccaa
    45481 tgatgccggt aattgttggt ccatggagca ctagtgaaat atccttaagt gccaacgttt
    45541 ctttgtagga gacactgagg ttttcgatac gtatcataaa cttgtattcc tcctgtctct
    45601 taatatacat taaaaaaaaa attaagtcaa gttaattttt gaaaaaatta aaataataac
    45661 tgaaaaatag attctaaaga taactttcag gataaatttc taaattataa aacgcatagt
    45721 atcaagtgta aaaaacttgg aattatgcgt tttatcatgg aaagattttt tataatagct
    45781 aaaaaataat agttaaatgg ttattaattg cattccctag tgatttttgt taagataaat
    45841 gcaaatacaa atgaaagcga gaacaagatg acacgttatc aagatgattt ttatgatgct
    45901 atcaatggag aatggcaaca gacagctgaa atcccagcag ataagtctca aacaggaggt
    45961 tttgttgatt tagaccagga aattgaagac ctgatgctgg cgacaacaga caagtggtta
    46021 gcaggtgaag aagtgcctga ggatgctatc ttggaaaact ttgtcaaata ccaccgccta
    46081 gttcgtgatt ttgacaagag agaagctgac ggtatcacac ctgtcttacc actccttaaa
    46141 gaattccaag aattggaaac ttttgcggat tttacagcta aactagcaga gtttgagctt
    46201 gcaggaaaac caaacttcct tccttttggt gtatcgccag actttatgga tgctagaatc
    46261 aatgttctat gggctagcgc tccaagcaca atcttgccag atacgaccta ctatgcagaa
    46321 gaacatcctc agcgcgaaga gctcttgact ctttggaaag aaagcagcgc aaatctcctc
    46381 aaggcttatg atttctctga tgaagaaatt gaagacttgc tagaaaaaag acttgaattg
    46441 gaccgccgag ttgtggcagt ggtgctctct aatgaagaaa gttcagaata tgctaaactc
    46501 tatcatccat attcttacga agatttcaag aaattcgcgc ctgccctacc tttggatgac
    46561 ttcttcaaag cagttattgg gcaattacca gacaaggtta ttgtagacga ggaacgtttc
    46621 tggcaagcag cagagcaatt ttacagtgag gaagcctggt ctctccttaa agcaaccttg
    46681 attttgagtg ttgtcaatct ttcaaccagc tatttaacag aggatatccg tgttttgtct
    46741 ggtgcctaca gccgtgccct ttctggagtt ccagaggcaa aagataaggt caaagcagct
    46801 tatcatctag cacaggaacc tttcaagcaa gccctggggc tttggtacgc ccgtgagaag
    46861 ttctctccag aagccaaggc ggatgtggag aaaaaagtgg caaccatgat tgatgtctat
    46921 aaggagcgtc tgcttaagaa tgactggctc actccagaaa cctgtaaaca ggctatcgtg
    46981 aagctcaatg tgatcaaacc ttatattggc tatccagaag aattgcctgc acgttacaag
    47041 gataaggtag tgaatgaaac tgccagtctt tttgagaatg ctctagcctt tgcgcgtgtg
    47101 gaaatcaagc acagttggag taagtggaac cagcctgtag actataagga atggggcatg
    47161 cctgctcata tggtcaatgc ctactacaat cctcagaaga acctgattgt ctttccagcg
    47221 gccattttac aggcgccttt ctatgacttg catcagtcat cttctgctaa ctacggtggt
    47281 attggggcag tgattgccca tgaaatttcc cacgcctttg atactaacgg ggcttccttt
    47341 gacgaaaatg gtagcctcaa ggattggtgg acagagagcg actatgctgc cttcaaggag
    47401 aaaacacaaa aagtcattga ccaatttgat ggacaggatt cttatggagc aaccattaac
    47461 ggtaaattga ctgtatcaga aaacgtggct gacttgggag gaatcgcagc agcgcttgaa
    47521 gcagctaaga gagaagcaga cttctcagca gaagagttct tctacaactt cggtcgcatc
    47581 tggcgcatga aaggtcgacc agaatttatg aaacttttgg ctagcgtcga tgtgcacgca
    47641 ccagccaaac tccgtgtcaa tgtgcaagta ccaaacttcg acgatttctt tacaacctat
    47701 gatgtcaaag aaggagacgg aatgtggcgt tcaccagagg agcgcgtgat tatttggtaa
    47761 tacaaaaaat ctagtcatca gaaaaaaggc atccaatcac gtgtgaaaac cttgatagga
    47821 tgccttttgt aatagaaaga tttgctggat agtttactta aatttcgatt gtcatcatct
    47881 tgctagaaaa agggattgaa ggccttttcg tgagcgatag tagtagctgg accatgtcct
    47941 ggatagacat cgtagtttgg tagggtgaag agttgggtct ggatactatg aaggagttgc
    48001 tccatgctac cagtcggaag gtcggtccgt ccgatagttt cgcggaatag agcatctccc
    48061 gtcaagacta ggtgagcatc aggaaagact agggaaacac caccgataga gtgccctggg
    48121 gtcggtagaa ccttaaaacg aaattcctct agttggtatt cttcgtgaaa gacaaaggtg
    48181 tgttctgcag gttttgtgac cacatctgcc atatcatcgt ggcgagggag accggagaga
    48241 ttatcgacag gagtgtagag ccagctggct tcgctctctg cgatatagac aggaggattg
    48301 ccaaacgtct cgcgaaccaa gtccagactc atgatatggt cataatgggc gtgggtcaag
    48361 agaatagcac agatcggttt gttgatcttc tcgattgtct gacgaatggc ttcccaatga
    48421 ctaccaggat cgacgacgat gaggtgcttt tcgccttcta gataataggt attttcatag
    48481 gcaacaggat tcacggtttt atggattttc atactagctc caatctcaaa gaatgtacta
    48541 atactcttcg aaaatctctt caaactgcgt caactttatc tgcaacctca aagcagtgct
    48601 ttgaacagcc tgcggctagc tttctagttt gctctttgat tttcattgag tataaattaa
    48661 gtatagcaca gttagggaga ataggtaagg atttaaaatg aacttaaaaa ccagcatgac
    48721 tagatgaaga caggctggtt atcaatttat caatattgga aaggtgtcaa ttgcccttca
    48781 taggttgcat aaactccgtc attagcttgt ttgatagacg tgatgtttag agtgctaccg
    48841 tagttggctt ctcgtttgtc gctatattca ctataagtta tcctactggg caggttctgg
    48901 tcgttagcat agtattgaac gactgttttt tatagctttg ctggctgaat aggaacaggc
    48961 agcaaactcc taacattagg agagcaaaga tagaaatagc agtttttttc ataggtattc
    49021 tccttataaa ctaaaggata gaagaaagta tcttgaggat ttttatactc ttcgaaaatc
    49081 tcttcaaacc gcgtcaacgt cgccttgccg tatatatgtg actgactt
//
LOCUS       DAVAAL010000002        48418 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVAAL010000002 DAVAAL010000000
VERSION     DAVAAL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.002"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(30..224)
                     /locus_tag="VQM60_000044"
     CDS             complement(30..224)
                     /locus_tag="VQM60_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET1079966.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(289..1425)
                     /gene="dnaN"
                     /locus_tag="VQM60_000045"
     CDS             complement(289..1425)
                     /gene="dnaN"
                     /locus_tag="VQM60_000045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET1079967.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(1584..2945)
                     /gene="dnaA"
                     /locus_tag="VQM60_000046"
     CDS             complement(1584..2945)
                     /gene="dnaA"
                     /locus_tag="VQM60_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET1079968.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(3158..3916)
                     /locus_tag="VQM60_000047"
     CDS             complement(3158..3916)
                     /locus_tag="VQM60_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET1079969.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEACAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(3974..5155)
                     /locus_tag="VQM60_000048"
     CDS             complement(3974..5155)
                     /locus_tag="VQM60_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET1079970.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            5338..5817
                     /gene="rlmH"
                     /locus_tag="VQM60_000049"
     CDS             5338..5817
                     /gene="rlmH"
                     /locus_tag="VQM60_000049"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET1079971.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            5861..5934
                     /locus_tag="VQM60_000050"
     tRNA            5861..5934
                     /locus_tag="VQM60_000050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5895..5897,aa:Arg,seq:tct)
     gene            6099..6224
                     /gene="comC"
                     /locus_tag="VQM60_000051"
     CDS             6099..6224
                     /gene="comC"
                     /locus_tag="VQM60_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET1079972.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            6245..7570
                     /locus_tag="VQM60_000052"
     CDS             6245..7570
                     /locus_tag="VQM60_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET1079973.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            7567..8319
                     /locus_tag="VQM60_000053"
     CDS             7567..8319
                     /locus_tag="VQM60_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1079974.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            8371..8442
                     /locus_tag="VQM60_000054"
     tRNA            8371..8442
                     /locus_tag="VQM60_000054"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8404..8406,aa:Glu,seq:ttc)
     gene            8448..8521
                     /locus_tag="VQM60_000055"
     tRNA            8448..8521
                     /locus_tag="VQM60_000055"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8482..8484,aa:Asn,seq:gtt)
     gene            complement(8562..9104)
                     /locus_tag="VQM60_000056"
     CDS             complement(8562..9104)
                     /locus_tag="VQM60_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET1079975.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            9211..10358
                     /locus_tag="VQM60_000057"
                     /pseudo
     CDS             9211..10358
                     /locus_tag="VQM60_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            complement(10388..12940)
                     /locus_tag="VQM60_000058"
     CDS             complement(10388..12940)
                     /locus_tag="VQM60_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET1079976.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(13002..14624)
                     /locus_tag="VQM60_000059"
     CDS             complement(13002..14624)
                     /locus_tag="VQM60_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET1079977.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            14830..15855
                     /gene="trpS"
                     /locus_tag="VQM60_000060"
     CDS             14830..15855
                     /gene="trpS"
                     /locus_tag="VQM60_000060"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET1079978.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            16007..17485
                     /gene="guaB"
                     /locus_tag="VQM60_000061"
     CDS             16007..17485
                     /gene="guaB"
                     /locus_tag="VQM60_000061"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET1079979.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(17536..18633)
                     /gene="recF"
                     /locus_tag="VQM60_000062"
     CDS             complement(17536..18633)
                     /gene="recF"
                     /locus_tag="VQM60_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET1079980.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(18636..19004)
                     /gene="yaaA"
                     /locus_tag="VQM60_000063"
     CDS             complement(18636..19004)
                     /gene="yaaA"
                     /locus_tag="VQM60_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HET1079981.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            19163..20413
                     /locus_tag="VQM60_000064"
     CDS             19163..20413
                     /locus_tag="VQM60_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET1079982.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLALDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILDETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERTYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            20410..21693
                     /locus_tag="VQM60_000065"
     CDS             20410..21693
                     /locus_tag="VQM60_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET1079983.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            21735..22556
                     /locus_tag="VQM60_000066"
     CDS             21735..22556
                     /locus_tag="VQM60_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET1079984.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            22567..23112
                     /gene="pgsA"
                     /locus_tag="VQM60_000067"
     CDS             22567..23112
                     /gene="pgsA"
                     /locus_tag="VQM60_000067"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET1079985.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIVLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            23109..23936
                     /locus_tag="VQM60_000068"
     CDS             23109..23936
                     /locus_tag="VQM60_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET1079986.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            23921..24760
                     /locus_tag="VQM60_000069"
     CDS             23921..24760
                     /locus_tag="VQM60_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET1079987.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            24753..25547
                     /locus_tag="VQM60_000070"
     CDS             24753..25547
                     /locus_tag="VQM60_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET1079988.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            25606..26424
                     /gene="mreC"
                     /locus_tag="VQM60_000071"
     CDS             25606..26424
                     /gene="mreC"
                     /locus_tag="VQM60_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET1079989.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            26424..26918
                     /gene="mreD"
                     /locus_tag="VQM60_000072"
     CDS             26424..26918
                     /gene="mreD"
                     /locus_tag="VQM60_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET1079990.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            27012..28190
                     /locus_tag="VQM60_000073"
     CDS             27012..28190
                     /locus_tag="VQM60_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET1079991.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            28414..29193
                     /gene="rpsB"
                     /locus_tag="VQM60_000074"
     CDS             28414..29193
                     /gene="rpsB"
                     /locus_tag="VQM60_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET1079992.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            29272..30312
                     /locus_tag="VQM60_000075"
     CDS             29272..30312
                     /locus_tag="VQM60_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET1079993.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            30558..30881
                     /gene="tnpB"
                     /locus_tag="VQM60_000076"
     CDS             30558..30881
                     /gene="tnpB"
                     /locus_tag="VQM60_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET1079994.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(31022..31330)
                     /locus_tag="VQM60_000077"
                     /pseudo
     CDS             complement(31022..31330)
                     /locus_tag="VQM60_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            31321..31719
                     /locus_tag="VQM60_000078"
     CDS             31321..31719
                     /locus_tag="VQM60_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET1079995.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(31916..32836)
                     /gene="cysK"
                     /locus_tag="VQM60_000079"
     CDS             complement(31916..32836)
                     /gene="cysK"
                     /locus_tag="VQM60_000079"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET1079996.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(33121..33756)
                     /locus_tag="VQM60_000080"
     CDS             complement(33121..33756)
                     /locus_tag="VQM60_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET1079997.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYTSSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            33812..35110
                     /locus_tag="VQM60_000081"
     CDS             33812..35110
                     /locus_tag="VQM60_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET1079998.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            35107..35769
                     /locus_tag="VQM60_000082"
     CDS             35107..35769
                     /locus_tag="VQM60_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET1079999.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            35849..36397
                     /gene="raiA"
                     /locus_tag="VQM60_000083"
     CDS             35849..36397
                     /gene="raiA"
                     /locus_tag="VQM60_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET1080000.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            36533..38506
                     /locus_tag="VQM60_000084"
     CDS             36533..38506
                     /locus_tag="VQM60_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET1080001.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            38503..38955
                     /gene="rplI"
                     /locus_tag="VQM60_000085"
     CDS             38503..38955
                     /gene="rplI"
                     /locus_tag="VQM60_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET1080002.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            38999..40351
                     /gene="dnaB"
                     /locus_tag="VQM60_000086"
     CDS             38999..40351
                     /gene="dnaB"
                     /locus_tag="VQM60_000086"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET1080003.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            40353..40619
                     /locus_tag="VQM60_000087"
     CDS             40353..40619
                     /locus_tag="VQM60_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080004.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            40708..42054
                     /gene="cbpD"
                     /locus_tag="VQM60_000088"
     CDS             40708..42054
                     /gene="cbpD"
                     /locus_tag="VQM60_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET1080005.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      42137..42239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            42310..42600
                     /locus_tag="VQM60_000089"
     CDS             42310..42600
                     /locus_tag="VQM60_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET1080006.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            42563..43321
                     /locus_tag="VQM60_000090"
     CDS             42563..43321
                     /locus_tag="VQM60_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1080007.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            43359..44366
                     /locus_tag="VQM60_000091"
     CDS             43359..44366
                     /locus_tag="VQM60_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET1080008.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            44366..45094
                     /locus_tag="VQM60_000092"
     CDS             44366..45094
                     /locus_tag="VQM60_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080009.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            45213..45671
                     /locus_tag="VQM60_000093"
     CDS             45213..45671
                     /locus_tag="VQM60_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET1080010.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLVSVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            45673..48105
                     /locus_tag="VQM60_000094"
     CDS             45673..48105
                     /locus_tag="VQM60_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET1080011.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
BASE COUNT        15120 a         7926 c        10114 g        15258 t
ORIGIN      
        1 taaagtgcta accctctaac taagggttct cagtcaataa ttttattcat ttttcgctca
       61 aaatcatatc gccccatcat gacaacatgc tcacaattac tgcattttat tttgatatct
      121 gctcctacac gtgtaatttc ccaacgatta gcctttttac cagtcgactt gattgtacaa
      181 gcgtgtgatt ttttcatctc aacaaaattt ccaacttgat acatactact ctccttttct
      241 ttttcgatta tatcataaaa gaggcgagcc aggctcaacc tctttcactt aatttgtacg
      301 aactggtgta atgagctgca tgaagtcttc gtcagtatct gctggcacaa gagtaaatgg
      361 acgaacagct gagataaagc taatggtcac cttttcgcta tttaaagctt taagagaatc
      421 aatcaagtaa gttgggttga aactaatggt caaatcttca ccagtaacct gatcagtatc
      481 gatttcttcg tttactttac caacttctgg agagtgaaca tgggcgctaa caaccccatc
      541 cttaatttca agtttcacag taccattttg agtcgcactt gataaaagac gggcacgctc
      601 cattgactgg cgtaagttta ccacattaaa agtaatagta gtgttaaagt ctgttggaat
      661 caagcgatct gtatcaggat agtttccttc taggagacga gtatagaagc taatattttc
      721 gcttctaaag aggatttggt tattggcaaa gaaaatctct acagtttcga tatcatctgt
      781 aaataccgct gaaaattcgc gtagagaacg gctaggaatt acgacatcaa aatcatcact
      841 atttttttca agagtcaatt ttttctggct taggcgatga gagtctgttg caactgtttt
      901 taactctttg tgttgactca atacgaagtg gacacctgtt aaaatcggac gactctcttg
      961 tgtacttgca gcaaaggctg tttcattaat aattttcttg agtaattttg tttcaagtat
     1021 taaaggagtg cttgctgaaa tttcttggat tcgtggatat tgttcgctat cttttccttt
     1081 tagggtaatt tctgatttgc cactggttaa aacaatttga ttttgttcaa tttctttaaa
     1141 atcaagagtc acatcaggta aactagatac tacattgata aagaaagaag cttcaagaag
     1201 gatcgaacct aaagaagtaa ttaacaaacc agcatcttca tttttttgag aaataaaatt
     1261 ttcaattgaa atttgaccat ttgaaccaat taaagtaata ccttcattgg tcacgtcaat
     1321 ttttactgtt gataaaatag gaatggcatt tttagaacta atagctctct tagtagtatt
     1381 taatgcttgt agaaataaat ttttattaat tgaaaaatga atcatggatt ctcctttatt
     1441 tatttttagt attagaaaga taatagtaat aatagagtct gtgaaatctg tggaaaacag
     1501 cctttaaaca agtcatatca agttttttag cttgtttaaa aaatgtggat aaaaaagata
     1561 aaaaactaaa agttatccac aaattatttg attttctttt tgattgattc aatttctaaa
     1621 cgtaaattat cgtcttgatc aatcaaagat tttattttgg catgggcatg aatgactgtg
     1681 gtatgatctt ttcccccaaa ttccttccca atttttggaa gactattatc tgttagttct
     1741 ctagataaat acatggctac ttgacgggcc aaaacaatat tttgaaggcg tctacttccc
     1801 ttcatttctt tgatactaac accataaaag ttaccaactt cagtttggat tttatcaatt
     1861 gggatgacga gcatttggct aacatcttgt ttgcgggctc taatggcttc tgcagcaata
     1921 tcaatagtga tatccttgat tttttttact ctggcaatta aagtgatgtc gttgatggct
     1981 ccctcaagat ctcgaacatt tgaatcaaat tgcccagcta ggtattctag agtatcactt
     2041 tggaaattgt agcctaaatg ttccgtctta ctttgtaaaa tggcaatacg tgtttcaaag
     2101 tcagggggtg tgatagtttg tgtcaatccc caactaaaac gcgtgacaag cctctcctcg
     2161 agcccttcta gatgttttgg actacgatca ctcgttagga caatctgttt ttgcttgtca
     2221 tgaagggcgt taaaggtatt gaaaaattct tcctgagttg cgactttttt tccgctgagt
     2281 gactgtatat catcgattaa caaaagatca agactacgat aggtcttttt aaacttttcc
     2341 atttccccaa gtcttaggtg atcaagaaag tcattaataa agctttcggc agggatatat
     2401 ttaacacgcg cattaggaat attttttaga atttcatttc caatagcgtt taataagtga
     2461 gtcttaccaa ggcctggtcc tccatagata aaaagagggt tataggtcag agccaaatct
     2521 tcagagacag ctaaagcggc tgatacagcc caaacatttc catccccttg aataaagtta
     2581 tcaaaggtat acttttcttt taatcccgta tctgaataag gaatagatac taactttgga
     2641 ctatagtcat aaagagttaa atttgtagct tcttcaactt gtgagctagt cgtatcttga
     2701 ggtttggtga aaatatagtg gggagttatt tcagcgtcat aaatttcaaa accagctact
     2761 acaataatat cttttagttg tttttcccag accatttcca tttcagagcg aggtagaaat
     2821 atagtggcaa cattttcctc taccttgatg agttcagctt gaatagcata gaaatcatac
     2881 atggatcgag tcagtctttc ttgtgcaaat tctaatatac gattccaaaa ttgtttttct
     2941 ttcaatcctt tctcctcctt tactatttaa aagtttaata ctagagatat tttaccatag
     3001 atagcaagaa ttttccacaa gctgtgaaaa ataatccaca atgttaccaa actttatcca
     3061 caggttgggg ataaaagaag aaattattga tttattaagc tttttatcga aaaacatagt
     3121 ggataacctt gtgagataaa aaaataaaag aacagcctta tttcaggctg ttgataattc
     3181 tactatattc ttcttgattt gaaaaagaaa taatgatttt tccactgtct tttttagata
     3241 gtttaatttc tacatctaat ccgagtagtt ttcttaactg tttttcttca ttttgtatga
     3301 aatgattagt tttttgctgt ttcttttgtt ttttctctgt cagaagagtt tctaacttcc
     3361 ttacagaaat atcttcttct ataatccgtt gaaagaaata gtcttgttgt tccttattta
     3421 acccaactag ggaacgcgca tgggcttgtg atagtttgcc attttctact tctgagagaa
     3481 tctgttctgg caaggaaagt aaacgaatgg agttgctgat atatggacga gacttgccca
     3541 ttttatctgc aatttcagca tgggtgaatc ctttctctac gagagattca taggcgcatg
     3601 cttcttctat tgggtttaaa ttttctctct gtaaattttc aataatggac tggaccatca
     3661 tctcttggtc tgaaatctgt ttaacaacag ctgggataga ccgtagacca gctaaaagtg
     3721 aagcccgata gcgtctctct cctgcaagga tttcataacc aataacagga gattgacgaa
     3781 caataatcgg ttgaatgacc ccattttctt tgatagactg tgctagttca tctagttttt
     3841 ctctatcaaa ttcttttcgg ggttgatagg gatttttttg tatatctgtg atagaaatca
     3901 tttcaaattt ttccatgatt ctacactaac acatcttttc tcttatgtaa agctttcttt
     3961 acatagatgt caattaagat tctaaatcac ctgaactctt gttaagtttg atagaggtag
     4021 tttcttcttt cccgttacga tagtaggtta tcttaatggt gtctccgata gaatggttgt
     4081 aaagagcact ttgtaagtct gttgatgaag caatctcttt gtcatctact tttgtaatta
     4141 catcgtattt ttcaaggtga ccattggcag gcatattact ttgtaccgaa cgaacaatta
     4201 caccagatgt aacattactt ggaatattga gtcttctgat gtcgcttgta ctcacattag
     4261 ataaattaac catctggatt cccaaagctg gacgcgtcac ttttccgttt ttttctaact
     4321 gttcaataat attgatagca tcatttgcag gaattgcgaa accaagacct tctacagatg
     4381 ttcctccatt tgtagcaatt ttacttgagg taattccgat aacctgccct tgaatattga
     4441 tcagtgggcc gccagagtta cctgggttaa tagcagtatc agtttggatg gcttttgtag
     4501 aaatagcttg tccatcttcc gattttaagg atacatttct attgagactg gatacgatac
     4561 cttgagtgac agtatttgca tattcagaac ctaacgggct accgatggca atagcagttt
     4621 ctcctacagt taacttacta gaatcaccaa actcagctac tgttgtcact ttttctgaag
     4681 agattttgac gacagcaata tcagagaaag tgtcagctcc gacaatttct ccaggtactt
     4741 tagtcccatc tgacaatcga atatctactt tgctggcgcc atttataacg tgattgttgg
     4801 tgacgatgta agcttcttta tcattctttt tataaataac tccagatcct tcactagaga
     4861 ttcgctgaga atctgtgtca gtatcatcat tgccaaatac gctattttgt ctgtttgccg
     4921 aataagtaat aacagaaaca acagcatctt ttactttgtt aacagcctgt gttgttgaat
     4981 tttcgttctt ataggcagtt tgtgtaatag tactattgtt gttagagttg tttacactac
     5041 ttttttgagt tagttgagtt attgaaaaac tacccaaggc tccactaaaa aagctaatga
     5101 cgataacgac taataattga aaccattttt tgtaaaatgt ttttagatgt ttcatatttg
     5161 cctccatatg tttgaattac tgaaagtata aactgactag cttaattata acttaaacac
     5221 aaaagtttta cacaaactgt ggataactct tttgaaactg tgattttctt aattgaaatc
     5281 tattttttat tttgtgaata agatgtgaaa aaatagagaa tatgttagaa tagagtcatg
     5341 aaaattaaag ttgtaacagt tgggaaactg aaagaaaagt atttaaaaga tggtatcgca
     5401 gagtattcaa aacgaatttc tagatttgct aagtttgaaa tgattgagtt atcagatgaa
     5461 aaaacaccag ataaggccag tgaatcagaa aatcaaaaga ttttagaaat agaaggtcag
     5521 agaattttat caaaaattgc tgaccgtgat ttcgttattg tgttagccat tgaagggaaa
     5581 actttcttct cagaagaatt tagtaagcag ttagaagaaa cttctataaa aggattttct
     5641 actcttactt ttattattgg gggaagttta ggattgtcat catctgtaaa aaatagagcc
     5701 aatctttctg tcagttttgg tcgcctaacc ttgcctcatc agttaatgag actagttctt
     5761 gttgaacaaa tctatcgcgc ttttacgatt cagcagggat tcccctacca taaatagaaa
     5821 attgactttt aattgaattt ttggtagaat aattgtgtta ggtctcatag ctcagctgga
     5881 tagagcattc gccttctaag cgaacggtcg caggttcgaa tcctgctggg atcaatataa
     5941 tagcaaagct gggaattttc ccggcttttt tcttaaaaaa gtacactttg ggagaaaaaa
     6001 atgacagttg agagaatttt atctaaaacg aaattccatt ttgtataatg gtttttgtaa
     6061 gttagcttac aagaaaaaac attttaggag attttattat gaaaaacaca gttaaattgg
     6121 aacagtttgt agctttgaag gaaaaagact tacaaaagat taaaggtggg gagatgagat
     6181 tgtcaaaatt cttccgtgat tttattttac aaagaaaaaa gtaatgaaat aaggggaaag
     6241 agtaatggat ttatttggat ttgggacggt tattgttcat tttttaatta ttagtcacag
     6301 ttaccatttt atttgtaaag gtcaaataaa tagaaaagaa ttattcgttt ttggtgctta
     6361 tacattacta acagaaatag tatttgattt tcccttatat attctatatt tagatgggtt
     6421 agggattgaa agatttttat ttcctttggg attatattcc tattttcgat ggatgaaaca
     6481 gtatgagagg gatagaggac tattcctaag tttactacta tctcttttat atgagagcac
     6541 tcataacttt ctgtccgtaa ctttctcctc tataacagga gataattttg ttttacaata
     6601 tcatttccca ttctttttcg ttgtgacggt gttaacctat tttgttacat taaaaatcat
     6661 ttactatttc catttggaac tagcctattt tgacgaggac tacctttatc ctttcttgaa
     6721 aaaagtattt tttgctttac tattgctaca tattgtatct ttcgtttcag atatggtaag
     6781 tacgattaaa catttgaata gttttggaag tattttgtca tctattgtct ttatctctct
     6841 ccttttgacc ttctttgcaa tgaattctca taaagttcaa atggagaaag agattgcttt
     6901 gaagcagaag aaatttgaac agaaacattt acagaattac acagatgaaa ttgttggtct
     6961 gtataatgaa atccgtggtt ttcgacatga ttatgctgga atgcttgtca gcatgcagat
     7021 ggcaattgac agtggtaatt tacaggaaat tgacagaatt tacaatgaag ttttagtcaa
     7081 agcaaatcat aaattgcgtt cagataagta cacttacttt gatttgaaca acatagaaga
     7141 ctcagcttta cgaagtttgg ttgctcagtc aattgtctat gctcgaaata atggtgtaga
     7201 gtttacactg gaagtaaaag atacgattac caagcttcca attgaactat tggatttggt
     7261 tcgtatcatg agcgttttat tgaataatgc tgtcgaagga tcggctgata gctataaaaa
     7321 gcagatggaa gtagcagtta ttaagatgga aactgaaaca gttattgtga ttcagaattc
     7381 atgtaaaatg acgatgactc cttcaggaga tctatttgcc ttaggattct ccactaaggg
     7441 aagaaatcgc ggagtcggat taaataatgt gaaagaacta ctagataagt acaacaatat
     7501 tattttagaa acagagatgg aaggcagtac atttagacaa atcattagat ttaagaggga
     7561 atttgaatga aagttttaat tttagaagat gttattgaac atcaagtgag actagagaga
     7621 atattggatg aaatttcgaa agaatcgaat attccaatat catacaagac aacgggaaaa
     7681 gtccgtgaat ttgaagaata cattgaaaat gatgaagtaa atcagcttta tttcctagat
     7741 atcgatattc atggaattga gaaaaaggga tttgaagtgg ctcagctcat tcgtcattac
     7801 aatccttacg ctattatcgt ctttatcact agtcgatcag agtttgcgac tctaacctat
     7861 aaataccagg tatcagccct agattttgtt gataaggata tcaatgatga gatgtttaag
     7921 aagagaattg agcaaaatat cttctacacg aagagtatgt tacttgaaaa tgaagatgtt
     7981 gtagattatt tcgactacaa ttacaaggga aatgatttaa aaattcctta ccatgatatt
     8041 ttgtatattg aaacaacagg ggtctctcat aaattgcgca ttattggtaa gaattttgca
     8101 aaagagtttt atggtaccat gacagatatt caggaaaagg acaaacatac tcagcgattt
     8161 tattctcctc acaagtcatt tttggtaaat ataggcaata tcagagaaat tgatcgaaaa
     8221 aacttagaaa ttgttttcta tgaagaccat cgttgtccta tttcaagatt aaaaattaga
     8281 aaattaaaag atattttaga gaaaaaatct caaaagtgat tgacaattag caagaaattg
     8341 atataatggt tatatctgct acagatagaa ggtccgttgg tcaaggggtt aagacaccgc
     8401 cttttcacgg cggtaacacg ggttcgaatc ccgtacggac tattttatcc gccatagctc
     8461 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactggcgga
     8521 gtatagataa aagggaacac agctgtgttc ctctttttgt atcaatttgt atcaccaagc
     8581 attttcataa ggaagtctgt tatttcttga gaactttctt tttttccatg tgcaatccaa
     8641 gtttggcaga caccaaaaag tgcatgagtt agatagatgc tactatattc taattcggtg
     8701 gtatttagat tcagttgcat aaatcgcttt tgtaaatctg tactaagcat gatatgaagt
     8761 ttatttcgta agaaattttg gatttcttta gtcccatttt cagaaagaag ggcagccaga
     8821 agtggttctg actctagata ttcaaaaact tctaaaatag cgtctctttt gtgatgagca
     8881 tgtttttgaa aaatatattc aaatgtatgg aatagcttgc tttgatagtg ctcaatcata
     8941 tcatacttat ccttatagtg agtatagaag ctggaacgac taattccggc tttttctact
     9001 aatttgacag tagaaatttt atcaaatggc tgttccatca gtaattgtac catagcattt
     9061 tcaatagttc gctttgtttt taagcgtttg ttactttctt gcatatttcc tccttgtaaa
     9121 caaattagac tatatgtcta aaaatagatt ttttatcttg taatttagat tttttaatgt
     9181 ataatctatt atatcaaaat tttagacaat atgtttaaaa aaggagaaac taagtttaaa
     9241 gaatggaaag caattttaaa aaaaaccaac ctttattatt gtcatgatcg ggatttctct
     9301 tattccagat ctgtacaata tcatattttt gtcatcaatg tgggatccat atgggcaatt
     9361 gtctgactta cctgtggcag ttgtaaataa tgataaagag gcttcctata atggtaatac
     9421 tatggcaata ggaaaagaca tggtgtccaa tttaaaagaa aataaaacct tggattttca
     9481 ttttgtagat gaagaggaag gaaagaaggg attggaagat ggcgattact atatggtagt
     9541 gactttacca agtgatttat ctgaaaaaac aactacatta tccaatattc aatcgacagc
     9601 agcttatcaa tcattgacaa gtgagcaaca aactgagata agtgattctg tatctcaaaa
     9661 tttaactgat agtattcaat cggctcagtc aattgtagct ttagtacaag atttacaggg
     9721 aagtttagaa aacttacaaa atcaatctaa tcaagtatca cctattactt ctacttcttt
     9781 gataggattg tcaagtggat taacagagat acaaggagat gttactagca aattagttcc
     9841 tgccagtcag tcgattgcat caggtgtaaa cgcatatact acaggtgttg ataaagtttc
     9901 tcaggacgtc atccagagag ccgttgggct tggttgaaat ctcgagctga aataaatggt
     9961 attatagctg ttttggcagg aattttggta tatggaggag ttcatcttat tggtttaact
    10021 gctaatcatg agatgagaat atttattctc atcatcctaa caagtttagt attcatgtct
    10081 atggtgacca ctttaacaac gtggaatagc cgtataggag ctttcttctc acttattttg
    10141 cttttactac agttagcatc aagtgcaggt acttatccac ttgctttgac aaatgatttc
    10201 tttagatcta ttaatccctg gttaccaatg agctattcag tttcgggatt acgacaaaca
    10261 atctccatca acaagtcatt ttcctagctg tcatactagc tctatttact ggtttaggta
    10321 tgctagccta tcaacataag aaaatggaag aagattaaaa aaatcgaccg attaactggt
    10381 cgatttttta tgccttagat gactttcgtc tgtgattata gattccaaat agtaagagag
    10441 aagtaaagga acagattgct ccagtaataa aaccattggg aatgaaggaa agtgtaatag
    10501 ttcctttccc cttgggaatg tcaactttca taaatccagt ttgagcttgt ttaatttcta
    10561 ttttcttacc atcttggtag gcagaccaac ctttgtcata aggaatggtg aagaaaatag
    10621 atgtatcttg ttggacatca tatgtagcaa aaaccttgtt tttagaagtt gatactgtga
    10681 caggttgttc tttaattttt tgaattgcct cggtgaaagt tttggtatct aaacgataga
    10741 aggtaggaga ttcaaatgat acttgtgaat ttccagggaa actaacattg atattgaaag
    10801 tttttttctc tttagtatat cctagattaa agaaggagaa gacattatca gttgtaaaag
    10861 tctttttttc accatttaca aggatgtcaa ccttcttttg tttatcgtta gaaaagtgaa
    10921 ggtttatgaa agagagataa acttggctgt tttctggaac ttcaatttga tactggattg
    10981 ctgcatcttc atttgaagaa cttgtgacac taatcaaatc attagtattt tctatttttt
    11041 ctgttttttc ataaggtatt ggagaaaaat aatcaaaatt gacgttagca agttgattta
    11101 aaaatgaggc ctgattatcc aaggtatgtt cattgaactt gacatcattg taaacagatt
    11161 gactagcaac tgcaatcgga agagagtatt gattttcata tagggtaaga ttatcttttt
    11221 gatagatatc tttaaaacca tacttatcaa taggactgtc tgagatattg tactggatac
    11281 caaataaact atcagccaaa atactattat ttgcatatcg gagattgaga ttagtcccag
    11341 aggatttaaa accaagttta tctaaagtag agcttgatga acgatttcga acagatgaaa
    11401 attgagagat tccattgtag ttgaatttca tactgtcatt tcctgtctga gtttgtagtt
    11461 tttcagtacg agtaaattga tttccaatat atgttgagaa agattccata gctgggatat
    11521 ctcgactata agcacttcga gaagcaaatc cccattcctt agcaattccg tccatttgag
    11581 atgaagcatt taaactcatt tcaaccagta taaataaaga gattagaatg gcaaatagat
    11641 tcacagatat aaactttttg ataactgcaa ggagtaaaag agaatagaca accaaaaatt
    11701 caagagtaag cagaatattc aaatctgtta aaaaagaata atgcgatttt agatagatgg
    11761 tagctaaaaa tcctgctact acaagaaaaa gcgaaactaa aaaattccag actttaagtt
    11821 ctttcagacg ctttaagact tctgctgctg tgtaaattaa caaggtagag aaaatccaag
    11881 catagcgatg taaaaacatg tttggagtat gcatgccttg ccaaaataag tcaagagctt
    11941 ctatgtaaaa gcttgcaatt agaaatgcaa agaatattac atatatgagt ttcacgtgaa
    12001 acttaataga tttcagcgta aaaaataaaa tggtcaaaat aaagggaagt agtccaacaa
    12061 aaatcattgg gatggcccca tactttgttg tgtcaaagga accaatgaat tgcttagcaa
    12121 agagatcaag ataccagcta ctttcagttt gaaactttgt aacttcagtc aatttttccc
    12181 catgtgtctg taaatcaaat agagtgggaa gagtcataat caaactagcc ataccagcta
    12241 aaaaggagat aactatgaaa tcaagaacag atgattttcg agtcttaaag tcccacgaaa
    12301 tttgacagag ataccagaaa ataagaaaca atactgtcat atatccaaaa taataatttt
    12361 gaataaataa gattgacaga cttgtaaagt acaataggag tttcttttca gttatcagta
    12421 gatgtaaacc agttataatt aaaggaatca agataaaaac atctagccag gtttttatct
    12481 ctaattgact gacagtgaaa ctcatcagag cataggaagt agataaggct agttttaaag
    12541 tctgagggat agattgaaac aatttattca aactaaaaaa ggttgacaga ccaatcaatc
    12601 caaattttaa gagagtggtc agatagacag catctggcat attcgttaga tcaaaaaagt
    12661 aaactagagg cgcgagaaaa ctacccaagt aataactaga tagggcatag aagtttagcc
    12721 ctagaccact tgtaaaggtg taaaacagac tactatttcc atgtaggata tttcgtaagg
    12781 ctacatcaaa aataacgtat tgatgaaagc catctcctaa tagaggagag ttgtcgctat
    12841 tccagtagat accttgagat agatatactc cagacataat cactacagga atgatgaaag
    12901 aaataaaata ggttcgatat gtttttaaaa atgatttcat gttacctcgt agaatgatag
    12961 aaaactcagt tggttaaccc aactgagttt tgaagtttta tttagtcttt ccaaagttct
    13021 ttaacttttg cttgtacttc tgcattttct aggaattcat cgtaggtttc atcgatacgg
    13081 tcaatgacgc catttttaga taagacaatg atatggttag ccaaagtttg aataaactcg
    13141 tggtcatggc tggcaaagat gattgattct ttaaagtttt tcaatccatc attcaagctt
    13201 gagatagatt ccaagtccaa gtgatttgtt ggatcatcaa gtacaaggac atttgatttt
    13261 aagagcatga gttttgaaag catgacacga actttttctc cccctgacaa gacatttaca
    13321 ggtttgttaa cttcatctcc agagaagagc atacggccga ggaagccacg taggaaagta
    13381 ttgtcatctt cttctttact tgcgaattga cgcaaccagt caagaattga ttctcctcct
    13441 gcaaaatcag ctgagttatc ttttggtagg taagattgac tagttgtaac tccccacttg
    13501 acagttcctt catagtcaat atctcccatg attgcacgaa ttaatgcagt cgtttgaata
    13561 tcattttgtc caataagtgc tgtcttatca tctggacgca agatgaaact aatattatcc
    13621 aagatagttt caccatcaat ctttacagtt agattttcta ctgtcaagag atcattacca
    13681 atctcacgtt ccgctttaaa gttgataaat ggatatttac gactagatgg cacaatctct
    13741 tctagctcaa tcttatcaag cattttctta cgtgatgttg cctgccttga cttagaagca
    13801 ttggcagaga aacgagcaac aaattcttgc aattgtttaa ttttttcttc tgctttagca
    13861 ttacggtctg ctagcaattt agcagcaagc tcagaagatt ccttccagaa gtcgtagttt
    13921 ccgacataga gtttgatttt tccaaagtca aggtcggcca tgtgagtaca aactttgttt
    13981 aagaagtgac ggtcgtggga tactacgata actgtgttat caaagtcaat taagaagtct
    14041 tctaaccaag taatcgattg gatatccaaa ccgttagtag gctcgtccaa gagaagaaca
    14101 tctggtttac caaaaagtgc tttggcgagg agaaccttta ctttttcacc gttggccaat
    14161 tcgctcatgt tttggtagtg taattcttct ggaatgttta ggttttgaag tagttgagag
    14221 gcttcactct ctgcttccca acctccaagt tcggcaaact ctccttcgag ttcggcagca
    14281 cgaaccccgt cctcgtctga gaaatcttcc ttcatgtaga tagcatcttt ctctttcatg
    14341 atgctataaa gtttttcatt tcccatgata acgacatcaa tggcacgttc atcttcgtag
    14401 tcaaagtgat tttgacgaag aacagagaga cgttcatctg gaccaagaga gatgtgacca
    14461 gtagtaggtt cgatatctcc agctaaaatt tttaaaaagg ttgattttcc ggcaccatta
    14521 gcaccgatta atccgtaagt atttccttct gtaaatttga tattgacatc atcaaaaagt
    14581 ttgcgatcac taaaacgtag tgaaacatca gatactgtaa gcaatatttt tctcctatat
    14641 gtgtaatata tttattctac tagaaaatac agaaatattc aaatttttat ttgtcaattt
    14701 tgtgtaaatt atatttacag tatcctttac acaaatctgt aaaaagcaag gctgatttat
    14761 tttgataaat tacggttatt tcattaaaaa aatgctataa ttgaaaggac tatatcgaag
    14821 gagaacaaaa tgactaaacc cattatttta acaggagacc gtccaacagg aaaattgcat
    14881 attggacatt atgttggaag tctcaaaaat cgagtattat tacaggaaga ggataagtat
    14941 gatatgtttg tgttcttggc tgaccaacaa gctttgacag atcatgccaa agatcctcaa
    15001 accattgtag agtctatcgg aaatgtggct ttggattatc ttgcagttgg attggatcca
    15061 aataagtcaa ctatttttat tcaaagccag attccagagt tggctgagtt gtctatgtat
    15121 tatatgaatc tagtttcgtt agcacgtttg gagcgaaatc caacagtcaa gacagagatt
    15181 tctcagaaag gatttggaga aagcattccg acaggattct tggtctatcc aatcgctcaa
    15241 gcagctgata tcacagcttt caaggctaat tatgttcctg ttgggacaga tcagaaacca
    15301 atgattgagc aaactcgtga aattgttcgt tcttttaaca atgcatataa ctgtgatgtc
    15361 ttggtagagc cggaaggtat ttatccagaa aatgagagag cagggcgttt gcctggttta
    15421 gatggaaatg ctaaaatgtc taaatcacta aataatggta tttatttagc tgatgatgcg
    15481 gatactttgc gtaaaaaagt aatgagtatg tatacagatc cagatcatat ccgcgttgag
    15541 gatccaggta agattgaggg aaatatggtt ttccattatc tagatgtttt tggtcgtcca
    15601 gaagatgctc aagaaattgc tgatatgaaa gaacgttatc aacgaggtgg tcttggtgat
    15661 gtgaagacca agcgttatct acttgaaata ttagaacgtg aactgggtcc tattcgtgag
    15721 cgccgtattg aatttgctaa ggatatggga gaagtttata atatgattca aaaaggtagt
    15781 gaaagagcgc gtgaagttgc gggtcaaacc ctatctgagg taaaaggggc aatgggactt
    15841 cattacttta actaagttta ttttacaaga aactatcatt tctatctcga agaaaagata
    15901 gttttttatt tacccctgta acatttgaca aatttttata gaatggtatg atagatacaa
    15961 tataaaaaga gtcaagctca aaaagaaaga aaagaggaaa cttcgaatgt ctaattggga
    16021 cactaaattt ttgaaaaaag gttttacctt tgatgatgta ttgcttattc cagctgaaag
    16081 tcatgtgttg cctaacgatg cagatttaac aactaaattg gcagataatt tgactttaaa
    16141 tatcccaatt attaccgctg ccatggacac agttacagag agtcaaatgg ccattgctat
    16201 tgctcgtgca ggtggtctcg gagttatcca taaaaacatg tcaattgctc aacaagcaga
    16261 cgaggttcgt aaggtaaaac gttctgaaaa tggagttatt attgatccgt tcttcttgac
    16321 gcctgaacat acaattgctg aagcagatga gcttatgggt cgttaccgca tcagtggtgt
    16381 tccagttgtt gaaacacttg aaaatcgtaa attggttggt attttgacaa accgagatct
    16441 tcgttttatt tcagattata atcaaccaat ttcaaaccat atgactagtg aaaatcttgt
    16501 tactgctcct gtgggtacgg atcttgcaac ggctgagagt attcttcaag agcatcgtat
    16561 tgaaaaactt ccgttggtcg atgaagaagg cagtctttct ggtttgatca ctatcaaaga
    16621 tattgaaaaa gttattgagt ttccaaatgc ggctaaagat gaatttggtc gtcttctagt
    16681 tgcaggtgca gtaggtgtta cttcagatac atttgaacgt gcagaggctc tttttgaggc
    16741 aggagcggat gcgattgtta ttgatactgc acatggtcat tctgcaggtg tcttgcgtaa
    16801 aattgccgag attcgtgctc atttcccaga tcggactttg attgctggaa atattgctac
    16861 tgctgaaggt gcacgtgccc tttatgaagc gggtgtagac gttgttaagg ttggtattgg
    16921 accaggttct atctgtacta ctcgtgtgat tgctggtgtt ggtgttccgc aagtaacagc
    16981 tatctacgat gctgcagctg ttgcgcgcga atatggtaaa acgattattg ctgacggtgg
    17041 gatcaagtat tctggagata ttgtaaaagc acttgctgca ggtggaaatg ctgttatgct
    17101 tggatctatg tttgctggaa ctgatgaagc tccaggcgaa actgaaatct tccaaggacg
    17161 taaattcaag acttaccgtg gtatgggatc aattgctgct atgaagaaag gttcaagcga
    17221 ccgttatttc caaggttctg tcaatgaagc aaacaagctc gttccagaag gaattgaagg
    17281 tcgtgttgct tataaaggag cggcagctga tattgttttc caaatgattg gtggtattcg
    17341 ctctggtatg ggttactgtg gtgcagctaa ccttaaagaa ctacacgata atgctcaatt
    17401 tattgaaatg tctggtgctg gtttgaaaga aagccatcct catgatgtgc aaattactaa
    17461 tgaggcacca aattattcta tgtaaaaaac aatgaaaaga actccagtga aaacaggagt
    17521 tcttttacaa tgttgtcaat ttccatttac agaagcttta ccatcctgaa tagtgaagat
    17581 acttagattt tctggcagat tttgaagatg gtctaagctt gttgttgtga taaaggtttg
    17641 gattgattga gaaatcgttt ctaataattt taactgtcta gtgttgtcaa gttcactcat
    17701 cacatcgtca agcagtaata taggagattc tgtggtaatg ctttccatta attcgatttc
    17761 tgctaatttt atcgagagga cgagactacg atgttgacct tggcttccga aactagcatc
    17821 catcccattt atataaaaag aaatgtcatc tcgatgagga ccgacaccag tattcttttt
    17881 aaataaatct ctggatctac ttttttctaa agcaattttg aaagattcgg ataagttttg
    17941 tttgtcagtt atattgacag aagattgata ggatattgac aactcttcga tctgattaga
    18001 gagttcaaaa tgtttcttac gcccaaatga ttctagtttt tttatgaaat ctaagcggtg
    18061 attcattaca cgacatccat aatcaactag ctgatcatct aacacagaaa ggaatgtttc
    18121 atctattttt tgagctgatt ttaggtaagt gtttctttgc tttaggatgt ggttataatt
    18181 ggttaagtca gataaataga ttggcttaat ttgcccaagt tccatatcaa tgaattttcg
    18241 tcgaatcgaa ggtgctcctt taattagttg taaatcttca ggagcaaata agacaacatt
    18301 catgtgtcct acataatctg aaaggcgtgc ctgttttaag tgattaactt ttgttacacg
    18361 gcctttttgt gttagttcga tttctagggg aatggatcca gtttttttct gaacgagacc
    18421 tgaaagatga agttgttcct catcaaaatg aatgagattt ttatctgttc gagttcgatg
    18481 actacgcgtt aaggctaaaa aatagatagc ctctaacatg tttgtttttc cttgtgcatt
    18541 acgtcccaaa aagacattca atttaggatt aaagtctatt ttcgtttctt tgtaattacg
    18601 aaaagtcttg agagatagat gttgtagcca catgattatc taccagggaa tcgtggagct
    18661 tgttggcttt taggtgatga agcaggtttc gatttgtctt ttttaactcc cttattcatc
    18721 tttttgacaa gtttagcgat ccgttctttt tcaactttat cagcttggta ttcatcttgt
    18781 tcttcagaag taggttgtgt caacaagatg tcaatgttca tgtcagggat gtcaacttta
    18841 tcgccaatac gaagtttttt tccacgacga ctctctaatt ccctattaaa gtaaacagaa
    18901 tgttcagaga gaaatgattt aatagctcct ccgctatgtg taattccaag ttctttgagt
    18961 agtgcttgga gggtaataaa ttcttcaaat aatttgtatt ccataattca cctcaatctt
    19021 tggtattata ccatattttc ctaaaaatag tgagagagac agcgagaaat gtaagcgatt
    19081 tttatttcaa aagtgttaca gggaacaaga aaatttcagg ttttcgtgat ataatagaag
    19141 tctgtatata aggaggtaaa tcatggagtt agtgcatgga atttcaacac attttatcca
    19201 atcaaaaaag tttaaaacaa acaaaattac cgtgcgtttt accgctccat tagcccttga
    19261 tacgattgca ggtcacatgt tgagtgcaag tatgctagag actgctaatc agatgtaccc
    19321 cacttctcaa gatttgagga gacacttggc cagtctatac ggtacagata tgtcaaccaa
    19381 ttgtttcaga agagggcaaa gccacattat agaattgaca tttacctatg ttcgtgatga
    19441 gtttttaagt aggaaaaacg tgctaacctc tcagattttg gaacttgtaa aagaaactct
    19501 tttttcaccc gtagtagttg ataatgggtt tgatccggcc ttatttgaaa ttgagaaaaa
    19561 acaattgcta gcaagtttag cagctgatat ggatgattct ttttattttg cacataaaga
    19621 attggataaa ttgttttttc atgatgaacg tcttcaattg gaatatagtg atttacgaaa
    19681 tcgtatttta gatgaaactc cacaaagttc ttattcttgt ttccaagaat ttttagccaa
    19741 tgatcgaata gatttctttt tcctaggtga ttttaatgag gttgaaattc aaaatgtatt
    19801 agaatcattt ggctttaaag gtcgaaaagg agatgtgaag gttcagtatt gtcaacctta
    19861 ttctaatatc cttcaggaag gtatggttcg gaaaaatgtg ggacaatcta ttttggaatt
    19921 aggttatcat taccgttcta aatatggtga tgagcaacat ttacccatga ttgtaatgaa
    19981 tggtttactt ggtggatttg ctcactctaa gctctttaca aatgtccgtg aaaatgctgg
    20041 attagcttat accatttcaa gtgagcttga tttatttagt ggattcttga ggatgtatgc
    20101 tggtatcaat cgagaaaatc gtaaccaggc tcgtaaaatg atgaataatc aactgcttga
    20161 tttaaaaaaa ggttatttta cagagtttga gttaaatcag accaaggaaa tgattcgttg
    20221 gtcgttgtta ctttctcaag ataatcaatc ttcattgatt gaacgtactt atcaaaatgc
    20281 cttatttgga aaatcttcag cagactttaa aagttggatt gcaaagcttg aacaaattga
    20341 caaagatgct atttgtagag tagctaataa tgtgaaacta caagcgattt actttatgga
    20401 aggaatagaa tgacaaaggt tgtttttgaa gaaaaatact atccagctgt aaaagaaaag
    20461 gtttatcgaa ctcgtttggc caacggattg acagttgctc ttttgcctaa aaaggaattt
    20521 aaagaggttt acgggagtgt cactgtacag tttggttcgg tagatacgtt tgtcacagaa
    20581 gttgacggag atgtaaaaca atatcctgga ggaattgctc attttcttga acataaatta
    20641 tttgagagag aagattctag tgatttgatg tcggctttta cgagtctagg tgcagatagt
    20701 aatgccttta caagctttac aaaaacaaac tatctttttt cagcaacgga ttatttttta
    20761 gaaaatttat atttacttga tgaattggta acatcagcac actttactga agcttccatt
    20821 ctgacagagc aggatattat tcagcaagaa cgagaaatgt accaagatga tccagattcg
    20881 tgtttattct tttcaacttt agcgaatttg tatcctggta cacctttagc aactgatata
    20941 gttggaagtg aggagtccat ttcccaaatc aatctaacta atttgcaaga aaattttaca
    21001 aagttttaca aacctgtaaa catgtctctg tttttagttg gtaattttga tgtggagcga
    21061 gtacaggact attttgaaag caaagaactg aaagattcag attttcagga agtagcaaga
    21121 gaaaagttgt ttttacagcc tgtaaagcca acagatagta tgagaatgga agtatcttct
    21181 cccaaactag cgattggagt tagaggtaag cgagaagttt ctgaagcgga ttgctatcga
    21241 catcatattt tattaaaatt attgtttgca atgatgtttg gttggacttc ggatcgtttt
    21301 caaaaatgtt atgaatcagg taaaattgat gcatccttat ctctggaagt tgaagtaaca
    21361 agtcgctttc attttgtcat gttgacaatg gatacgaaag agccagttgc tttgtctcat
    21421 caatttagga aggctattcg taattttaca aaggatttag atattacaga ggaacattta
    21481 gatattatca aaagagagat gtttggcgaa tttttcagta gcatgaactc tcttgaattt
    21541 attgcaacgc aatatgatgc ttttgaaaat ggtgagacaa tttttgattt gccgaaaatt
    21601 ttacaggaaa ttactttaga ggatgtcctt gatgctggac atcatttaat agatgatggt
    21661 gacatagttg attttacaat attcccatcg tagtaaccta ttataataga cactagaaag
    21721 aagggatgac aagtatgaga aaaaaaacaa ttggagaggt tttacgatta gctagaatca
    21781 atcagggatt gagtttagat gaattgcaga aaaagacaga aatccagtta gatatgttgg
    21841 aagcaatgga agcagacgat ttcgatcaac ttccaagtcc tttttacacg cgttctttct
    21901 tgaaaaaata tgcatgggct gttgagttag atgaccaaat tgttttggat gcttatgatt
    21961 ctgggagtat gattacttat gaggaagtag atgttgatga agatgagttg acaggtcgta
    22021 gacgttcaag taagaaaaag aagaaaaaaa catcattttt acctttattt tattttatcc
    22081 tttttgcttt atcgatttta atttttgtga cttattatgt ttggaactat attcaaactc
    22141 aaccagagga gccttctctt tctaattaca gtgtggttca atcaacaagt tcaactagct
    22201 ctgttcccca ctcctcaagt agtagttctt ctagtataga atcagctata agtgtatcag
    22261 gcgaaggaaa tcatgtagaa atcgcttata agacaagtaa ggaaacagtt aaattgcaat
    22321 tggcagtttc agatgttaca agttgggtca gtgtttcaga aagcgaactt gagggcggtg
    22381 taaccttatc gccaaagaag aaaagtgcag aagcaacagt tgcaactaaa agtcctgtaa
    22441 caattacgtt aggtgttgta aaaggtgttg atttgacagt agataatcag actgttgatt
    22501 tatcgaaatt aacagctcag actggacaaa ttactgtaac ctttactaaa aattaaggaa
    22561 aaacgaatga aaaaagaaca aattcccaat ctcttaacaa taggtcgaat tctctttata
    22621 cctattttta tctttatttt aacgatagga aattcgatag agagtcatat agttgcagct
    22681 attatctttg ctgttgccag tattaccgac tatttagatg gatatttagc tcgtaaatgg
    22741 aatgtggtca gtaattttgg taaatttgca gatcctatgg cggataagtt actagttatg
    22801 tcggctttta ttgtgttgat tgagttaggt atggctccgg cttggattgt tgcagtgatt
    22861 atctgtcgtg agttagctgt gacaggttta aggcttttat tggttgaaac tggtggaaca
    22921 attttagcag cagcaatgcc tggaaaaatt aaaactttta gtcagatgtt tgccattatt
    22981 ttcttgctat tacattggac tttgcttggt caagttctac tttatgtagc cttatttttc
    23041 actatctact ctggctatga ctatttcaag ggtagtgcct atgtatttaa agggacattt
    23101 ggttcgaaat gaaatcaata attgatgtaa aaaatctttc ttttcgctat aaagaaaatc
    23161 agaactacta cgatgtgaag gatattacgt ttcacgtgaa acgtggagaa tggctttcga
    23221 ttgtagggca taatggtagt ggtaaatcaa cgacggttcg attaattgat ggcttactgg
    23281 aagcagaatc cggagagatt gtaattgatg gccaacgttt gactgaggaa aatgtttgga
    23341 atatacgtcg tcaaatcggt atggtttttc aaaatccaga caatcaattt gttggagcga
    23401 ctgttgaaga tgatgttgct tttggtttgg aaaatcaggg actttctcgt caagaaatga
    23461 aaaagagagt agaagaagct ctggctttag ttggcatgtt ggactttaaa aagagagagc
    23521 cagcgcgtct atcaggtggc caaaagcaac gtgtggccat tgcaggtgtt gtagccctaa
    23581 gaccagctat tttaatctta gatgaagcaa cgagtatgtt ggatcctgag gggcgtagag
    23641 aacttattgg gacagtaaaa ggaattcgaa aagactatga tatgacggtc atttctatta
    23701 cccatgattt ggaagaagtc gccatgagtg atcgcgtatt ggtcatgaaa aaaggggaaa
    23761 ttgaatcaac tagtagtcca agggagcttt tctctcgaaa tgatttagat caaattggat
    23821 tagacgatcc ttttgctaat caattaaaaa aatctttgag ccagaatggc tatgatttac
    23881 ctgaaaatta tttgacagaa agtgagctag aggataagct atgggaattg ctctagaaaa
    23941 tgtgaatttt acatatcaag aaggtactcc cttagcttca gcagctttgt cggatgtttc
    24001 tttgacgatt gaagatggct cttatacagc tttaattggg cacacaggta gtggtaaatc
    24061 aactatttta caactcttaa atggtttatt ggtgccaagt caagggagtg tgagggtttt
    24121 tgatacctta atcacctcga cttctaaaaa taaagatatt cgtcaaatta gaaaacaggt
    24181 tggcttggta tttcagtttg ctgaaaatca gatttttgaa gaaacggttt tgaaggacgt
    24241 tgcttttgga ccgcaaaatt ttggagtttc tgaagaagat gctgtgaaga ctgcgcgtga
    24301 gaaactggct ctggttggaa ttgatgaatc actttttgat cgtagtccgt ttgagctgtc
    24361 agggggacaa atgagacgtg ttgccattgc aggcatactt gccatggagc cagctatatt
    24421 agtcttagat gagccaacag ctggtctaga tcctctaggg agaaaagagt tgatgaccct
    24481 gttcaaaaaa ctccaccagt cagggatgac catcgtcttg gtaacgcatt tgatggatga
    24541 tgttgctgaa tatgcaaatc aagtctatgt aatggaaaag ggacgtttag taaagggggg
    24601 caaaccaagt gatgtctttc aagacgttgt ttttatggaa gaagttcagt tgggagtacc
    24661 taaaattacg gccttttgta aacgattggc tgatagaggc gtgtcattta aacgattacc
    24721 gattaagata gaggagttta aggagtcgct aaatggatag tatgatttta gggcgttata
    24781 tcccagggga ttcgattgtt caccgattgg atccacgtag caaattgctt gctatgatgc
    24841 tactgatttt gattgtgttt tgggctaata accccttgac gaatctgatt ctttttatag
    24901 cgacagggat atttattgcc ttgtcaggag tatctctttc attttttatt cagggcttga
    24961 agtctatgtt tttcttgatt gccttcacaa ctatttttca actatttttc atttctaatg
    25021 ggaatgtttt atttgagttt tcgtttgtga gaatcacgga ttatgctttg caacaagctg
    25081 ggattatttt ttgtcgcttt gtattgatta ttttcttttc aactttgtta accttaacaa
    25141 ccatgccttt aagtttggca tcagctgtcg aagctttatt agcaccttta aagcgtgtga
    25201 aagttccagt tcatgaaatt ggattgatgc tgtctatgag tttgcgtttt gtcccaacct
    25261 tgatggatga tacgacgcgg attatgaatg cacagaaagc tcgtggagtg gattttggag
    25321 aaggaagcat cgttcaaaaa gtaaaggcga tgattcccat tttgattcct ctttttgcga
    25381 caagtttaaa acgtgcagat tccttggcta tcgctatgga agcgcgtggc tatcagggtg
    25441 gaaaaggcag aagtcaatac cgacaattga aatggactct aaaggatacg ctgaccattc
    25501 ttgttattct cgtacttggt tgttgtttat ttttcttaaa atcttagtag ctttcaggaa
    25561 ttgataaaaa gttactgtaa cagtttttga tataaaggta gggatatgaa ccgttttaaa
    25621 aaatcaaaat atgtcattat tgtttttgtc actgttctgc ttgtgtcagc tctcttagcg
    25681 acgacttatt caagtacaat tgtgacaaaa ttaggagatg gaatctcatt ggttgataga
    25741 gttgtacaaa aaccttttca gtggtttgat tctgtcaaat cagatttggc tcatttgaca
    25801 cgaacatata atgaaaatga aagtttgaag aaacagcttt accaattaga agttaaatca
    25861 aatgaggtgg aaagtttaaa gacagaaaat gaacaactgc gccaattgct tgatatgaag
    25921 tctaaattgc aagccacaaa gactttagca gcagatgtta ttatgcgttc tccggtatct
    25981 tggaagcagg agttgacctt agatgcaggt agatcaaaag gtgcttctga gaacatgtta
    26041 gctattgcaa atggtggctt gattgggagt gtttcaaaag tagaggagaa ctctactata
    26101 gtcaaccttc tgacaaatac ggaaaatgct gataagattt ctgttaaaat tcaacatggc
    26161 tctactacaa tttatggaat tattattggc tatgacaagg aaaatgacgt tcttaaaatt
    26221 agccaattaa atagtaatag cgatattagt gcgggagata aggtgactac tggtggatta
    26281 ggaaacttta acgttgctga tattcctgtt ggtgaagtgg ttgccacaac gcatagtaca
    26341 gactatttga cacgagaagt aactgttaaa ttgagtgcag atactcataa tgtagatgtg
    26401 atagaattag tggggaattc ataatgagac agttgaagcg agttggagta tttttattgc
    26461 ttcctttctt tgttctaatt gacgcccata ttagccagct tctgggctca tttttccccc
    26521 atgtacattt ggctagtcat tttctttttc tatttctctt atttgagacg atagaagtat
    26581 cagagtatct ctacctagtc tattgttttg ttataggctt ggtttatgat gtttactttt
    26641 tccatctaat agggattaca actctcttat ttatcttatt gggagccttc cttcataaat
    26701 tgaatagtgt tattttgttg aatcgttgga caagaatgct agctatgatt gtgctgacat
    26761 tcctgtttga aatgggtagt tatcttttgg cttttatggt agggttgaca gtagatagca
    26821 tgtcgatttt tatagtctat agcttggtac cgacgatgat tttaaatttt ttatggatta
    26881 ctgtttttca atttattttt gaaaaatatt atctataaga acgacatata aatgtaacaa
    26941 aggcgtaata tttattaggc ctttttttgg tatactagta ttgtctttaa aagaaggagt
    27001 atctacgtaa tatgaagaaa aaaatcttag cgtcactttt attaagtaca gtaatggttt
    27061 ctcaagtagc tgttttaaca actgcgcatg cagaaacgac tgatgacaaa attgctgctc
    27121 aagataataa aattagtaac ttaacagcac aacaacaaga agcccaaaaa caagttgatc
    27181 aaattcagga gcaagtatca gctattcaag ctgagcagtc taacttgcaa gctgaaaatg
    27241 atagattaca agcagaatct aagaaactcg agggtgagat tacagaactt tctaagaaca
    27301 ttgtttctcg taaccaatcg ttggaaaaac aagctcgtag tgctcaaaca aatggagccg
    27361 taactagcta tatcaatacc attgtaaact caaaatcaat tacagaagct atttcacgtg
    27421 ttgctgcaat gagtgaaatc gtatctgcaa acaacaaaat gttagaacaa caaaaggcag
    27481 ataaaaaagc tatttctgaa aaacaagtag caaataatga tgctatcaat actgtaattg
    27541 ctaatcaaca aaaattggct gatgatgctc aagcattgac tacgaaacag gcagaactaa
    27601 aagctgctga attaagtctt gctgctgaga aagcgacagc tgaaggggaa aaagcaagtc
    27661 tattagagca aaaagcagca gctgaggcag aggctcgtgc agctgcggta gcagaagcag
    27721 cttataaaga aaaacgagct agccaacaac aatcagtact tgcttcagca aacactaact
    27781 taacagctca agtgcaagca gtatctgaat ctgcagcagc acctgtccgt gcaaaagttc
    27841 gtccaacata cagtacaaac gcttcaagtt atccaattgg agaatgtaca tggggagtaa
    27901 aaacattggc accttgggct ggagactact ggggtaatgg agcacagtgg gctacaagtg
    27961 cagcagcagc aggtttccgt acaggttcaa cacctcaagt tggagcaatt gcatgttgga
    28021 atgatggtgg atatggtcac gtagcggttg ttacagctgt tgaatcaaca acacgtatcc
    28081 aagtatcaga atcaaattat gcaggtaatc gtacaattgg aaatcaccgt ggatggttca
    28141 atccaacaac aacttctgaa ggttttgtta catatattta tgcagattaa tttacagagg
    28201 gactcgaata gagccctctt ttcaggtttt accgtgacaa tccctattaa aaattatatc
    28261 aaaatagctt gaaaatattg gaaaagtatg gtagaatgaa aattgtcgtg tgaacgataa
    28321 tactcattct tgatgaattg tgaagcagtt gcccttgggt cgttttgcga gttgaagtca
    28381 agaagaggaa aaaaacaaaa aggagaaata ctcatggcag taatttcaat gaaacaactt
    28441 cttgaggctg gtgtacactt tggtcaccaa actcgtcgct ggaatcctaa gatggctaag
    28501 tacatcttta ctgaacgtaa cggaatccac gttatcgact tgcaacaaac tgtaaaatac
    28561 gctgaccaag catacgactt catgcgtgat gcagcagcta acgatgcagt tgtattgttc
    28621 gttggtacta agaaacaagc agctgatgca gttgctgaag aagcagtacg ttcaggtcaa
    28681 tacttcatca accaccgttg gttgggtgga actcttacaa actggggaac aatccaaaaa
    28741 cgtatcgctc gtttgaaaga aattaaacgt atggaagaag atggaacttt cgaagttctt
    28801 cctaagaaag aagttgcact tcttaacaaa caacgtgcgc gtcttgaaaa attcttgggc
    28861 ggtatcgaag atatgcctcg tatcccagat gtgatgtacg tagttgaccc acataaagag
    28921 caaatcgctg ttaaagaagc taaaaaattg ggaatcccag ttgtagcgat ggttgacacc
    28981 aatactgatc cagatgatat cgatgtaatc atcccagcta acgatgacgc tatccgtgct
    29041 gttaaattga tcacagctaa attggctgac gctattatcg aaggacgtca aggtgaggat
    29101 gcagtagcag ttgaagcaga atttgcagct tcagaaactc aagcagattc aattgaagaa
    29161 atcgttgaag ttgtagaagg tgacaacgct taatttatac aaatagtaat tacctaggag
    29221 ggcggggctt agcccggctc tcctattttc aaaaaatata ggagaattaa aatggcagaa
    29281 attacagcta aacttgtaaa agagttgcgt gaaaaatctg gtgccggtgt tatggacgct
    29341 aaaaaagcgc ttgtagaaac agacggtgac atcgaaaaag cgattgaatt gcttcgtgaa
    29401 aaaggtatgg ctaaggcagc taagaaagct gaccgtgttg ctgcagaagg tttgactggt
    29461 gtttatgtta acggtaatgt tgcagcagtt attgaagtaa acgctgaaac tgacttcgtt
    29521 gcaaaaaacg ctcaattcgt tgaattggta aatactacag ctaaagtcat tgctgaagga
    29581 aaacctgcta acaatgaaga agctcttgct ttgataatgc cttcaggtga aactcttgaa
    29641 gctgcatacg tatctgcaac agcaactatc ggagagaaaa tctcattccg tcgctttgca
    29701 ttgattgaaa aaacagacgc acaacacttt ggagcatacc aacataacgg tggacgtatc
    29761 ggtgttattt cagttgttga aggtggagac gaagcacttg ctaaacaatt gtcaatgcac
    29821 atcgcagcga tgaaaccaac agttctttct tacaaagaat tggatgagca attcgttaaa
    29881 gatgagttgg cacaattgaa tcacgttatc gaccaagaca acgaaagccg tgcaatggtt
    29941 aataagccag ctcttccaca cttgaagtat ggatcaaaag ctcaattaac tgatgatgtt
    30001 attgctcaag ctgaagctga catcaaagct gaattggctg cagaaggcaa accagaaaaa
    30061 atctgggaca aaattattcc aggtaaaatg gatcgcttca tgcttgataa cactaaagtt
    30121 gaccaagctt acacacttct tgcacaagtt tacatcatgg atgacagcaa gacagttgaa
    30181 gcataccttg aatcagttaa cgcttcggta gttgagtttg ctcgctttga agttggtgaa
    30241 ggtatcgaga aagctgcaaa cgacttcgaa gctgaagttg cagctacaat ggcagcagcc
    30301 ttgaataact aataatagaa aggaagcaat ttgcttcctt ttttgtatag ggcttatatt
    30361 tttcctgaaa ttgtttttcc cccagtctca ctcgattaga ataattaaaa caaaaagccc
    30421 tataataagg gctaagtgca tttaggagac tatacttcaa attcatagta agcgcgtcat
    30481 aacaaggtat ctatcctaat tccccaactt ccagtatact agataaaaaa taattatcgg
    30541 aggaaaggta ttgtactatg atgattcaac taagtgattt aggtcaagtt cacattgttt
    30601 gtggcaagac agatatgagg cagggaatag actcattagc ctatgtagtt aaaacccact
    30661 ttgaattgga ttctttctcc ggtcaagtct ttctcttttg tggtggacgt aaagaccgct
    30721 ttaaagtcct ttactgggat ggtcaaggat tttggctact atataaacgc tttgagaacg
    30781 gaaaactgac ttggctaagt acagaaaagg atgtcaaagc tctcacatct gaacaagtag
    30841 actggcttat gaagggcttt tctatcactc cccaaatata gtggtttgaa actagaatag
    30901 tacacctctg cttctaaaac attgttagaa atcgatttga ctgtcctgtt cttatttcat
    30961 tttactataa attcatcaga aagtcgtgat ttctattgaa atgaggactt tctttttata
    31021 ctcatctgtt ttcacaaagc attctagtcc atctccgatt aacgatggac tttatcacct
    31081 ccttctccag tccttgtata acatcttgga gttgattcat gacatcttcc aaaatttgaa
    31141 aggctttatt cttaaatcca cgtttacgaa tctctttcca cacttgttca atggggttca
    31201 tctctggtgt gtatggagga ataaatgcaa agccaatatt agtcggaatc tttaaggtac
    31261 ttgatttatg ccatatagca ttgtccataa cgagtaaaag ataatcatct ggataagctt
    31321 gtgaaatctc ctattcctaa agccccttta gcgcataact ttggctcagc ttctattatc
    31381 gctcacacca tccatcagaa gtttaatctg aaggtaccca attatcgcca agaagaagat
    31441 tgggctagga tgggtttacc aatcacacgt aaggaaatct ctaatcggca tatcaagacg
    31501 agtcaatact atttggagcc cctttataac ctcttgcgag agagactatt gactcagccc
    31561 ttacttcatg cggatgaaac ttcttatagg gtgctagaga gtgatagtca gctgacttac
    31621 tattggactt ttttgtctgg gaaagctgag aaacaaggga ttacgcttta ccatcatgtc
    31681 ctaatcgatt tgttcatatc ttatttcaat ccattataaa tagtgagaaa tatctatcct
    31741 atcttctaga atgtcttcca aacgtggaaa ctctcgtaaa caaagaggtt ttagaggcct
    31801 atttaccatg gactaaagtt gtacaagaaa agtgcaaata agaaatctcc agattaggaa
    31861 ctatccgtga gttctctagt ctggagattt ttcaatagac ttcgttattg gacggttaca
    31921 attcataaag tgctgtagag agataacgtt caccgttatc tggtgctagg gcaaggactt
    31981 ttttacctgt acctaatttt ttggcaacct cgatggctcc gtagatagct gcagctgagg
    32041 aaatccctac aaggaagcct tcttttccac caatttcacg tccgagtgca agagcgtcat
    32101 ctgatgttac acgaacgata ccatcatagg ctttagtatc aagtgtatca ggaataaatc
    32161 cagctgagat accttgaatt ttgtgaggac caggtttttc accagataga atagcagatt
    32221 catctgcttc tactgcaaaa acttgaatgt tagaattttc tgatttgagt gcatgagaaa
    32281 caccagaaat cgttccacca gtacctactc cagcaacaaa ggcatctaat ccatctttac
    32341 cgaaagcagc tagtatctca gctcctgttg ttctttcgtg tacttctgga ttagctggat
    32401 tgtcaaattg aagaggaagg aaaccatcac gttcagcagc gatttcttga gccttagcaa
    32461 tagcaccttt cattccctcg ctaccaggag ttaggacgag ttcagcacca taagcttgga
    32521 taattttacg tcgttctaca ctcatagttt caggcataac gatgacgact ttataccctt
    32581 tagcagcacc tacccatgaa agtccaatac cggtgtttcc acttgttgct tcaacaatag
    32641 tagaaccaag tttcagaata ccatcttgtt cagctttttc aatcatgcta agggcaatac
    32701 ggtcttttac agatgaacca ggattaaatg cttcaagctt tatatagacg tctgcagcac
    32761 cttctggcac gatgttgtta agtttaacaa tcggtgtttg accgattaat tcagtaatgt
    32821 tgttataaat agacatatga atacctcttt gtataagata tctttagtat aacgctatcc
    32881 ataccatttg taaaatatag aaatcctatc gaagcgatag gatttctata tatcatagat
    32941 caaagtcatt aattttcaag tgattgcgtt cagttaattc tagtaaatag gtgtgaagga
    33001 aaacgatatc aggtttcgtt tgaattgctt cattttctag tatattttgg tacgcatctt
    33061 tttattttgc tcgctaaagt agttgtgaga atgtcaacga aacgctgtat tattcattgt
    33121 ttacactaag tttacaggaa cttcaacctc tcgtaaacct tggtcagtta aagtgacttt
    33181 tccattaaaa aactccacaa gtgcagcttt aatagtttct ttttcttctt tatcaacata
    33241 aatcatcgta tcgacttgat ctgtaaagtt tgtatccagc tccatgagat catgttcttt
    33301 aaggaagtta ctgtactctt ggtactgagc ataagacatt tgaatagcaa tgccagcctg
    33361 ttcttttatt tcaataatac caatttcttt gacagctaag gcgacactgc tggtgtaagc
    33421 acgaattagt cctccagcgc ctagtttaat accaccaaag tagcgtgtca cgaccacaca
    33481 gacattggtg agattgtgat tttctagtac cccaagcatg ggaacaccag cagtaccact
    33541 aggctcacca tcatcacttg tacgtttaat ttcactacgt tctccaataa taaaggcaga
    33601 gcagttatgt gtcgctttgt agtgttcttt tttgatggca gtaatgaagt cacgagcctc
    33661 ttcttcgcta taaacacgct tggcatggca gataaagcga gattttttga tttcttcttg
    33721 gacttggccg tcctctttaa ttgtcctaaa ttccatgatt taattgtact aaaaaatcat
    33781 ctaaagtgct agtttttacg aataaagaag tatgaaagta aatttagatt atctcggtcg
    33841 tttatttact gagaatgaat taacagaaga agaacgtcag ttggcggaga aacttccagc
    33901 aatgagaaag gagaagggga aacttttctg tcaacgttgt aatagtacta ttctagaaga
    33961 atggtatttg cccatcggtg cttactattg tcgagagtgc ttgctgatga agcgagtcag
    34021 aagtgatcaa actttatact attttccgca ggaggatttt ccgaagcaag atgttctcaa
    34081 atggcgcggc caattaactc cttttcaaga gaaggtgtca gagggactgc ttcaagcagt
    34141 agacaagcaa aagccaacct tagttcatgc ggtaacagga gctggaaaga cagaaatgat
    34201 ttatcaagta gtggctaaag tgatcaatgc gggtggtgca gtgtgtttgg ctagtcctcg
    34261 catagatgtt tgtttggagc tgtacaagcg cctgcaacag gatttttctt gcgggatagc
    34321 tttgctacat ggagaatcgg aaccttattt tcgaacacca ctagttgttg caacaaccca
    34381 tcagttattg aagttttatc aagcttttga tttgctgata gtggatgaag tagatgcttt
    34441 tccttatgtt gataatccca cgctttacca tgctgtcaag aatagtgtaa aggagaatgg
    34501 attgagaatc tttttaacag cgacttcgac caatgagtta gataaaaagg tccgtttagg
    34561 agaactaaaa agactgaatt taccgagacg gtttcatgga aatccgttga ttattccaaa
    34621 accaatttgg ttatcggatt ttaatcgcta cttagacaag aatcgtttgt caccaaagtt
    34681 aaagtcctat attgagaagc agagaaagac agcttatccg ttactcattt ttgcttcaga
    34741 aattaagaaa ggggagcagt tagaagaaat cttacaggag caatttccaa atgagaaaat
    34801 tggctttgta tcttctgtaa cagaggatcg attagagcaa gtacaagctt ttcgagatgg
    34861 agaactgaca atacttatca gtacgacaat cttggagcgt ggagttacct tcccttgtgt
    34921 ggatgttttc gtagtagaag ccaatcatcg tttgtttacc aagtctagtt tgattcagat
    34981 tggtggacga gttggacgaa gcatggatag accgacagga gatttgcttt tcttccatga
    35041 tgggttaaat gcttcaatca agaaggcgat taaggaaatt cagatgatga ataaggaggc
    35101 tggtctatga agtgcttgtt atgtgggcag actatgaaga ctgttttaac ttttagtagt
    35161 ctcttacttc tgaggaatga tgactcttgt ctttgttcag actgtgattc tacttttgaa
    35221 agaattgggg aagagaactg tccaaattgt atgaaaacag agttgtcaac aaagtgtcaa
    35281 gattgtcaat tttggtgtaa agaaggagtt ggagtcagtc atagagcgat ttttacttac
    35341 aatcaagcta tgaaggattt tttcagtcgg tataagtttg atggagactt cctgttaaga
    35401 aaagttttcg cttcattttt aagtgaggag ttgaaaaagt acaaagagta tcaatttgtt
    35461 gtaattcccc taagtcctga tagatatgct aatagaggat ttaatcaggt tgagggcttg
    35521 gtagaggcag caggctttga gtatctggat ttattagaga aaagagaaga gagagccagt
    35581 tcttctaaaa atcgttcaga gcgcttgggg acagaacttc ctttctttat taaaagtgga
    35641 gtcactattc ctaaaaaaat cctacttata gatgatatct atactacagg agcaactata
    35701 aatcgtgtta agaaactgtt ggaagaagct ggtgctaagg atgtaaaaac attttccctt
    35761 gtaagatgag gaaaaaattt gcaaaaatag aatgaaagcg ttataataaa atcataaaaa
    35821 caaaaatgtt tagaaagaag gtactcatat gattaaatat agtatccgtg gtgaaaacct
    35881 agaagtaaca gaagcaattc gtgattatgt agtttctaaa ctcgaaaaga tcgaaaagta
    35941 cttccaacca gaacaagagt tggatgcccg aattaactta aaagtttatc gtgaaaaaac
    36001 ggctaaagtg gaagtaacga ttccgcttgg atctattact ctccgcgcag aagatgtatc
    36061 tcaagatatg tatggttcaa ttgaccttgt aactgataaa attgaacgtc agattcgtaa
    36121 aaataaaaca aaaatcgagc gtaaaaataa aaataaggta gcaactggtc aattatttac
    36181 agatgctttg gtggaagatt caaatattgt ccagtctaaa gttgttcgtt caaaacaaat
    36241 tgatttaaaa ccaatggatt tggaagaagc aattctacaa atggatttat tggggcatga
    36301 tttctttatc tatgtggatg ttgaagatca gacaaccaat gtgatttatc gtcgtgagga
    36361 tggcgaaatt ggtttgttag aggttaaaga atcttaattt caatatgtgt aaaggtaggt
    36421 ttactgaatt gtaaactacc tttttcttat aattgataga attactgatt acacttttaa
    36481 aaagtcgctt tttgaggggt attatggtat aatgggtgcc aagaggtttt gaatgaaaaa
    36541 attttatgta agtccaattt ttcctattct agtaggattg attgcgtttg gagtcttatc
    36601 cactttcatt atttttgtta ataataatct gttgacggtt ttaattttgt ttctttttgt
    36661 aggaggctat gtttttttat ttaagaaact gagagtgcat tatacaagga gtgatgtaga
    36721 acagatacag tatgtaaacc accaagcgga agaaagtttg acagctctat tggaacagat
    36781 gcctgtaggt gttatgaaat tgaatttatc ttctggagag gttgagtggt ttaatcccta
    36841 tgctgaattg attttgacca aggaagatgg tgattttgat ttagaagctg ttcaaacgat
    36901 tatcaaggct tcagtaggaa atccgtctac ttatgccaag cttggtgaga agcgttatgc
    36961 tgttcatatg gatgcttctt ccggtgtttt gtattttgta gatgtatcca gggaacaagc
    37021 cataacagat gaattggtaa caagtagacc agtgattggg attgtctctg tggataatta
    37081 tgatgatttg gaggatgcaa cttctgagtc agatattagt caaatcaata gttttgtagc
    37141 taattttata tcagagtttt cagaaaaata catgatgttt tctcgtcggg taagtatgga
    37201 tcgattttat ctatttactg actacacggt gcttgagggc ttgatgaatg ataaattttc
    37261 tgttattgat gctttcagag aagagtcgaa acagagacag ttgcccttga ccttaagtat
    37321 gggattttct tatggcgatg gaaatcatga tgagataggg aaagttgctt tgctcaattt
    37381 gaacttggct gaagtacgtg gtggcgacca ggtggttgtt aaggaaaacg acgaaacgaa
    37441 aaatccagtt tattttggtg gtgggtctgc tgcttcaatc aagcgtacac ggactcgtac
    37501 gcgcgctatg atgacagcta tttcagataa gattcggagt gtagatcagg tttttgtagt
    37561 cggtcacaaa aatttagaca tggatgcttt gggctctgct gtaggtatgc agttgttcgc
    37621 cagcaatgtg attgaaaata gctatgctct ttatgatgaa gaacaaatgt ctccagatat
    37681 tgaacgagct gtttcattca tagaaaaaga aggagttacg aagttgttgt ctgttaagga
    37741 tgcaatgggg atggtgacca atcgttcttt gttgattctt gtagaccatt caaagacagc
    37801 cttaacatta tcaaaagaat tttatgattt atttacccaa accattgtta ttgaccacca
    37861 tagaagggat caggattttc cagataatgc ggttattact tatatcgaaa gtggtgcaag
    37921 tagtgccagt gagttggtaa cggaattgat tcagttccag aattctaaga aaaatcgttt
    37981 gagtcgtatg caagcaagtg ttttgatggc tggtatgatg ttggatacta aaaatttcac
    38041 ctcgcgagta actagtcgga catttgatgt tgctagctat ctcagaacgc gcggaagtga
    38101 tagtattgct atccaggaaa tcgctgcgac agattttgaa gaatatcgtg aggttaatga
    38161 acttatttta caggggcgta aattaggttc agatgtacta atagcagagg ctaaggacac
    38221 gaaatgctat gatacagttg ttattagtaa ggcagcagat gccatgttag ccatgtcagg
    38281 tattgaagcg agttttgttc ttgcgaagaa tacacaagga tttatctcta tctcagctcg
    38341 aagtcgtagt aaactgaatg tacaacggat tatggaagag ttaggcggtg gaggccactt
    38401 taatttggca gcagctcaaa ttaaagatgt aaccttgtca gaagcaggtg aaaaactgac
    38461 agaaattgta ttaaatgaaa taaaggaaaa ggagaaagaa gaatgaaagt aatcttttta
    38521 gcagatgtta aaggaaaagg taaaaaaggc gaaattaagg aagtaccaac agggtatgcg
    38581 caaaactttc ttatcaaaaa gaatctagcc aaagaagcga ctgctcaagc tgtaggtgaa
    38641 cttcgtggta aacaaaaatc ggaagaaaaa gctcacgctg agatgattgc agaagggaaa
    38701 gcaattaaag cacaacttga agcagaagaa actgttgtag aatttgttga aaaagttggt
    38761 ccagatggtc gtacctttgg ttctattacc aataagaaga ttgcagaaga attacaaaag
    38821 caatttggaa ttaagattga taaacgtcat attcaagtac aagctccgat tcgagcggtt
    38881 ggtttgattg atgtgccagt gaaaatctat caagatatca caagtgtaat caatcttcgt
    38941 gtgaaagaag gataagttta caccttcttg acaagattgt aaaaggaagg gaagtctgat
    39001 ggcagaagta gaagagttac gagtacaacc tcaagatatc ttagctgagc aatccgtttt
    39061 aggggctatc tttattgatg agagtaaact tgtttttgtg cgagaataca ttgagtctcg
    39121 ggactttttt aagtatgccc atcgtttgat tttccaagcc atggtcgatt tatccgatcg
    39181 tggtgatgcc atagatgcaa caacggttcg tactatcctt gataatcaag gtgatttaca
    39241 gaatattggt ggcttgtctt acttggttga gattgtcaat tctgtgccaa cttctgctaa
    39301 tgcggagtat tatgctaaga ttgttgcaga aaaagcaatg ctacgtcgtt taattgccaa
    39361 gttgacagag tctgtcaacc aagcttacga agcgtcacaa ccagctgatg aaattattgc
    39421 tcaggcagaa aaagggctga ttgatgtcag tgaaaatgca aatcgaagcg ggtttaagaa
    39481 cattcgagat gtgttgaatc tcaactttgg aaatctggaa gctcgctcgc aacaaacgac
    39541 cgatattaca ggtattgcga caggttatcg tgatttggat catatgacaa caggacttca
    39601 tgaggaggag ttgattatct tagcagctcg tccagcagtt ggtaagacag catttgcctt
    39661 gaatatcgct cagaatattg ggactaagtt ggacaaaacg gttgctattt tttcactcga
    39721 aatgggtgcg gaaagcttgg tagaccgtat gttagctgca gaaggcttgg tggagtcaca
    39781 ttctatccgt acagggcaat tgacagatga ggagtggcaa aaatatacta ttgctcaggg
    39841 taatctagct aacgccagta tctatatcga tgatacgcca ggtattcgga ttacagagat
    39901 tcgttctcgt tctcgtaaat tggctcaaga aactggaaat cttggtttga ttttgataga
    39961 ctatttgcaa cttatcacgg gaactggtcg agaaaatcgt caacaagaag tttctgaaat
    40021 ttctcgtcag ttgaaaatac tagccaagga attgaaggtt ccagtaatcg ctctgagtca
    40081 gctttctcgt ggtgtagaac aacgtcagga caagagaccg gtcttgtctg atattcgtga
    40141 atctgggtct attgagcagg acgctgatat cgtagctttt ctctatcgcg atgactacta
    40201 tgaacgtggt ggtgaagaag aggagggtat cccaaataat aaggtggaag ttattatcga
    40261 gaaaaaccgt agtggagctc gtggaacagt ggaattgatt ttccaaaaag aatacaataa
    40321 attttcaagt atctcaaaga gggaggcata agatgtcaga tgcatttaca gatgtagcca
    40381 agatgaaaaa aatcaaagaa gaaatcaagg cacatgaggg acaagtcgta gaaatgactt
    40441 tggagaatgg tcgtaagcgc caaaaaaata gattgggtaa gctaattgaa gtttatccat
    40501 ccctatttat tgtggagttt ggggatgtgg aaggagataa acaagttaat gtttacgttg
    40561 aatcctttac ttactcagat attcttacag aaaagaattt gattcattat cttgactaaa
    40621 gtgagaaatt ttctcacttt ttcttttttc tccgaataat ttaggtgaag gcaatcatcg
    40681 ctttatatta ttttttaagg aggaagaatg aaaattttac cgtttatagc aagaggaaca
    40741 agttattact tgaagatgtc agttaaaaag cttgttcctt ttttagtagt aggattgatg
    40801 ctagcagctg gtgatagtgt ctatgcctat tccagaggaa atggatcgat tgcgcgtggg
    40861 gatgattatc ctgcttatta taaaaatggg agccaggaga ttgatcagtg gcgcatgtat
    40921 tctcgtcagt gtacttcttt tgtagccttt cgtttgagta atgtcaatgg ttttgaaatt
    40981 ccggcagctt atggaaatgc gaatgaatgg ggacatcgtg ctcgtcggga aggttatcgt
    41041 gtagataata caccgacgat tggttccatt acttggtcta ctgcaggaac ttatggtcat
    41101 gttgcctggg tgtcaaatgt aatgggagat cagattgaga ttgaggaata taactatggt
    41161 tatacagaat cctataataa acgaattata aaagcaaaca cgatgacagg atttattcat
    41221 tttaaagatt tggatggtgg cagtgttggg aatagtcaat cctcaacttc aacaggcgga
    41281 actcattatt ttaagaccaa gtctgctatt aaaactgaac ccctagttag tgcaactgtg
    41341 attgattact attatcctgg agagaaggtt cattatgatc aaattctcga aaaagacggc
    41401 tacaagtggt tgagttatac tgcctataat ggaagctatc gttatgttca attggaggct
    41461 gtgaataaaa atcctctagg taattctgtt ctttcttcaa caggtggaac tcattatttt
    41521 aagaccaagt ctgctatcaa aactgaaccc ctagttagtg caactgtgat tgattactat
    41581 tatcctggag agaaggttca ttatgatcag atacttgaaa aagacggcta caagtggttg
    41641 agttatacgg cttataacgg aagtcgtcgc tatatacagc tagagggagt gacttcttca
    41701 caaaattatc agaatcaatc aggaaatatc tctagctatg gatccaataa tagttcaact
    41761 gtcggttgga agaaaataaa tggtagttgg tatcatttca aatcaaatgg ttctaaatca
    41821 acaggatggc tgaaagatgg ttctagctgg tattatttga aattatctgg tgaaatgcag
    41881 acaggatggt taaaggagaa tggctcgtgg tattatctgg gtagttcagg ggcaatgaaa
    41941 acaggctggt accaggtctc tggtaagtgg tattattctt actcttcagg cgccttagct
    42001 gttaatacga cggtggatgg ctacagagta aacagtgatg gagaacgagt atagaaaatt
    42061 ggagtaggag aaatttcctg ctcttttctg taagcagttt ccttgacatt tttcttattt
    42121 tgcgctatca tatatacata taatatatgg gagtctgtgt acagtctgag aggaagtgtt
    42181 aaacttcgac cgcacctgat ctgggtaatg ccagcggagg gaacgatact tagtctaatt
    42241 ttgcaccttt tccatgtatg gtaaaggttt ttcttttttt taaaaggaaa acgagaagag
    42301 gaggttctta tgaaagcaag cattgccttg caagttttac ccctagcaca ggggattgat
    42361 cggatagctg ttattgatca ggtcattgct tatctgcaaa ctcaagaagt gacgatggta
    42421 gtgacaccat ttgaaacggt cttggaaggg gagtttgatg agcttatgcg cattctaaaa
    42481 gaagcgctgg aagtggcagg gcaggaggca gacaatgtct ttgccaatgt caaaataaat
    42541 gtaggagaga ttttaagtat tgatgagaaa cttgaaaagt atactgagac gacacattag
    42601 tctattgggc tttctcggag tattgtcaat ctggcagtta gcaggttttc ttaaacttct
    42661 ccccaagttt atcctgccga cacctcttga aattctccag ccctttgttc gtgacagaga
    42721 atttctctgg caccatagct gggcgacctt gagagtggct ttactggggc tgattttggg
    42781 agttttgatt gcctgtctta tggctgtgct catggatagt ttgacttggc tcaatgacct
    42841 gatttaccct atgatggtgg tcattcagac cattccgacc attgccatag ctcctatcct
    42901 ggtcttgtgg ctaggttatg ggattttgcc caagattgtc ttgattatct taacgacaac
    42961 ctttcccatc atcgttagta ttttggacgg ttttaggcat tgtgacaagg atatgctgac
    43021 cttgtttagt ctgatgcggg ccaagccttg gcaaatcctg tggcatttta aaatcccggt
    43081 tagcctgcct tacttttatg caggtctgag ggtcagtgtc tcctacgcct ttatcacaac
    43141 tgtggtatct gagtggttgg gaggttttga aggtcttggt gtttatatga ttcagtctaa
    43201 aaaactgttt cagtatgata ccatgtttgc cattattatt ctggtgtcga ttatcagtct
    43261 tttgggtatg aagctggtcg atatcagtga aaaatatgtg attaaatgga aacgtactta
    43321 aaatagagcg tttttgaaaa agaaaagagg aaatcaaaat gaagaaaaca tggaaagtgt
    43381 ttttaacgct tgtaacagct cttgtagctg ttgtgcttgt ggcctgtggt caaggaactg
    43441 cttctaaaga caacaaagag gcagaactta agaaggttga ctttatccta gactggacac
    43501 caaataccaa ccacacaggg ctttatgttg ccaaggaaaa aggttatttc aaagaagctg
    43561 gagtggatgt tgatttgaag ttgccaccag aagaaagttc ttctgacttg gttatcaacg
    43621 gaaaggcacc atttgcagtg tatttccaag actacatggc taagaaattg gaaaaaggag
    43681 caggaatcac tgccgttgca gctattgttg aacacaatac atcaggaatc atctctcgta
    43741 aatctgataa tgtaagcagt ccaaaagact tggttggtaa gaaatatggg acatggaatg
    43801 acccaactga acttgctatg ttgaaaacct tggtagaatc tcaaggtgga gactttgaga
    43861 aggttgaaaa agtaccaaat aacgactcaa actcaatcac accgattgcc aatggcgtct
    43921 ttgatactgc ttggatttac tacggttggg atggtatcct tgctaaatct caaggtgtag
    43981 atgctaactt catgtacttg aaagactatg tcaaggagtt tgactactat tcaccagtta
    44041 tcatcgcaaa caacgactat ctgaaagata acaaagaaga agctcgcaaa gtcatccaag
    44101 ccatcaaaaa aggctaccaa tatgccatgg aacatccaga agaagctgca gatattctca
    44161 tcaagaatgc acctgaactc aaggaaaaac gtgactttgt catcgaatct caaaaatact
    44221 tgtcaaaaga atacgcaagc gacaaggaaa aatggggtca atttgacgca gctcgctgga
    44281 atgctttcta caaatgggat aaagaaaatg gtatccttaa agaagacttg acagacaaag
    44341 gcttcaccaa cgaatttgtg aaataatgac agaaattaga ctagagcacg tcagttatgc
    44401 ctatggtcag gagaggattt tagaggatat caacctacag gtgacttcag gcgaagtggt
    44461 ttccatccta ggcccaagtg gtgttggaaa gaccaccctc tttaatctaa tcgctgggat
    44521 tttagaagtt cagtcaggga gaattgtcct tgatggtgaa gaaaatccca aggggcgcgt
    44581 gagttatatg ttgcaaaagg atctgctctt ggagcacaag acggtgcttg gaaatatcat
    44641 tctgcccctc ttgattcaaa aggtggataa ggcagaagct atttcccgag cggataaaat
    44701 tcttgcgacc ttccagctga cagctgtaag agacaagtat cctcatgaac ttagcggtgg
    44761 gatgcgccag cgtgtagcct tactccggac ctaccttttt gggcacaagc tctttctctt
    44821 agatgaggcc tttagcgcct tggatgagat gacaaagatg gaactccacg cttggtatct
    44881 tgagattcac aagcagttgc agctaacaac cctgatcatc acgcatagta ttgaggaggc
    44941 cctcaatctc agcgaccgca tctatatctt gaaaaatcgc cctgggcaga ttgtttcaga
    45001 aattaaacta gattggtctg aagatgagga caaggaagtc caaaagattg cctacaaacg
    45061 tcaaattttg gcggaattag gcttagataa gtagaaaaat agggagttgg tgaagattat
    45121 cctttaccag cgcccttttt cttttaaaaa tgagaaaatt tcggtataat agtcaaacaa
    45181 ggtcaaggtt taaagagaga ggtgggtttg ttatgagatt taaaaataca tcggatcata
    45241 ttgaggccta catcaaggcg attttagatc aatctggtat cgtggagttg caacggagtc
    45301 agttggcaga tacctttcag gttgttccta gtcagattaa ctacgtgatc aagacacgct
    45361 ttacggaaag tagaggctac ttggttgaaa gtaagcgtgg tggcggaggc tacattcgta
    45421 taggacggat tgagttttct agtcatcatg aaatgctccg ggagctgctt tactcgattg
    45481 gtgagcgagt cagtcaagaa atttatgagg atattctcca gcttttggtt gagcaggaat
    45541 tgatgaccaa gcaggagatg aatttgctag tatcagtagc tttggatcgc gttttaggag
    45601 aagaagctcc agttgttcga gcaaacatgc tacgtcagat catacaagaa gtagatagaa
    45661 aagggaagta agatgaacta ttcaaaagca ttgaatgaat gtatcgaaag tgcctacatg
    45721 gttgctggac attttggagc tcgttatcta gagtcttggc acttgttgat tgccatgtct
    45781 aatcacagtt atagtgtagc aggggcaact ttaaatgatt atccgtatga gatggaccgt
    45841 ttagaagagg tggctttgga actgactgaa acggactata gccaggatga aacctttacg
    45901 gaattgccgt tctcccgtcg tttgcaggtt ctttttgatg aagcagagta tgtagcgtca
    45961 gtggtccatg ctaaggtact agggacagag cacgtcctct atgcgatttt gcatgatggc
    46021 aatgccttgg cgactcgtat cttggagagg gctggttttt cttatgaaga caagaaagat
    46081 caggtcaaga ttgctgctct tcgtcgaaat ttagaagaac gggcaggctg gactcgtgaa
    46141 gatctcaagg ctttacgcca acgccatcgt acagtagctg acaagcaaaa ttctatggcc
    46201 aatatgatgg gcatgccgca gactcctagt ggtggtctcg aggactatac gcatgatttg
    46261 acagagcaag cgcgttctgg caagttagaa ccagtcatcg gtcgggacaa ggaaatctca
    46321 cgtatgattc aaatcttgag ccgtaagact aagaacaacc ctgtcttggt tggggatgct
    46381 ggtgtcggga aaacagctct ggcgcttggt cttgctcagc gtattgctag tggtgacgtg
    46441 cctgcggaaa tggctaagat gcgcgtgtta gaacttgatt tgatgaatgt cgttgcaggg
    46501 acacgcttcc gtggtgactt tgaagaacgc atgaataata tcatcaagga tattgaagaa
    46561 gatggccaag tcatcctctt tatcgatgaa cttcacacca tcatgggttc tggtagcggg
    46621 attgattcga ctctggatgc ggccaatatc ttgaaaccag ccttggcgcg tggaactttg
    46681 agaacggttg gtgccaccac tcaggaagaa tatcaaaaac atatcgaaaa agatgcagca
    46741 ctttctcgtc gtttcgctaa agtgacgatt gaagaaccaa gtgtggcaga tagtatgact
    46801 attttacaag gtttgaaggc gacttatgag aaacatcacc gtgtacaaat cacagatgaa
    46861 gcggttgaaa cagcggttaa gatggctcat cgttatttaa ccagtcgtca cttgccagac
    46921 tctgctatcg atctcttgga tgaggcggca gcaacagtgc aaaataaatc aaagcatgta
    46981 aaagcagacg attctgactt gagtccagct gacaaggcct tgatggatgg caagtggaaa
    47041 caggcagccc agctaatcgc aaaagaagag gaagtgcctg tctataaaga cttggtgaca
    47101 gaatctgata ttttgaccac cttgagtcgc ttgtcaggta tccccgttca aaaactgact
    47161 cagactgatg ccaagaaata cttaaacttg gaagctgaac tgcacaaacg tgtcatcggt
    47221 caagaccaag ctgtttcaag tattagtcgt gccattcgcc gcaatcagtc aggaattcgc
    47281 agtcacaagc gtccgatcgg ttcctttatg ttcctaggac ccacaggtgt cggtaagacc
    47341 gagttggcca aggctttggc agaagttctc tttgatgacg aatcagccct tatccgcttt
    47401 gatatgagtg agtatatgga gaaattcgca gccagccgtc taaatggagc tcctccaggc
    47461 tatgtgggtt acgaagaagg tggggagttg acagagaagg ttcgcaataa accctattcc
    47521 gttctcctct ttgatgaggt agagaaggcc catccagata tctttaatgt tctcttgcag
    47581 gttctggatg acggtgtctt gacagatagc aagggacgca aggtcgattt ttcaaatacc
    47641 attatcatta tgacatcgaa cttaggtgcg actgcccttc gtgatgataa gactgttggt
    47701 tttggggcta aggatattcg ttttgaccag gaaaatatgg aaaaacgcat gtttgaagaa
    47761 ctgaaaaaag cttatagacc ggaattcatc aaccgtattg atgagaaggt ggtcttccat
    47821 agcctatcta gtgaccatat gcaggaagtg gtgaagatta tggtcaagcc tttagtggca
    47881 agtttggctg aaaaaggcat tgacttgaaa ttacaagctt cagctctgaa attgttagca
    47941 aatcaaggat atgacccaga gatgggagct cgcccacttc gcagaaccct gcaaacagaa
    48001 gtggaggaca agttggcaga acttcttctt aagggagatt tagtggcagg cagcacactt
    48061 aagattggtg tcaaagcagg ccagttaaaa tttgatattg cataaaagaa taaaagtatc
    48121 agcatctgac cataagtcac agtggagtga aactcaatga aaatcaaaga gcaaactagg
    48181 cagctagccg caggttgctc aaaacactgg tttgaggttg cagatagagc tgacgtggtt
    48241 tgaagagatt ttcgaagagt atgaaactaa aacctatagc ttctaaacga tccgtggttt
    48301 tcaccattca acacaaaatt catatgttta ttaccctccg tcgtatttgt cttagagcgt
    48361 gtgtagtaga aaaagagcag tcttatctga aatttttatt ctttcaaaag agacctgt
//
LOCUS       DAVAAL010000003        41086 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVAAL010000003 DAVAAL010000000
VERSION     DAVAAL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.003"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(78..911)
                     /locus_tag="VQM60_000095"
                     /pseudo
     CDS             complement(78..911)
                     /locus_tag="VQM60_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(901..2748)
                     /gene="uvrC"
                     /locus_tag="VQM60_000096"
     CDS             complement(901..2748)
                     /gene="uvrC"
                     /locus_tag="VQM60_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET1080012.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEIYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(2788..3489)
                     /locus_tag="VQM60_000097"
     CDS             complement(2788..3489)
                     /locus_tag="VQM60_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET1080013.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(3568..4800)
                     /gene="murN"
                     /locus_tag="VQM60_000098"
     CDS             complement(3568..4800)
                     /gene="murN"
                     /locus_tag="VQM60_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET1080014.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(4805..6025)
                     /gene="murM"
                     /locus_tag="VQM60_000099"
     CDS             complement(4805..6025)
                     /gene="murM"
                     /locus_tag="VQM60_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET1080015.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(6137..6916)
                     /locus_tag="VQM60_000100"
     CDS             complement(6137..6916)
                     /locus_tag="VQM60_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET1080016.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYMALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(6985..8646)
                     /locus_tag="VQM60_000101"
     CDS             complement(6985..8646)
                     /locus_tag="VQM60_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET1080017.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(8821..11043)
                     /gene="recJ"
                     /locus_tag="VQM60_000102"
     CDS             complement(8821..11043)
                     /gene="recJ"
                     /locus_tag="VQM60_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET1080018.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEERENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            11320..12078
                     /locus_tag="VQM60_000103"
     CDS             11320..12078
                     /locus_tag="VQM60_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET1080019.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            12118..12882
                     /locus_tag="VQM60_000104"
     CDS             12118..12882
                     /locus_tag="VQM60_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET1080020.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNSKTIGVAQGSITQCLITELGKKKGLTFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            12895..13572
                     /locus_tag="VQM60_000105"
     CDS             12895..13572
                     /locus_tag="VQM60_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET1080021.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGFFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            13582..14241
                     /locus_tag="VQM60_000106"
     CDS             13582..14241
                     /locus_tag="VQM60_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET1080022.1"
                     /translation="MESILEILTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVHNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYKAGMSQGFTSAQILYYIILPQASRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            14536..15726
                     /locus_tag="VQM60_000107"
     CDS             14536..15726
                     /locus_tag="VQM60_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET1080023.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISSPYLGKITHLKRLNHDTREIQIHLCRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(16047..16928)
                     /locus_tag="VQM60_000108"
     CDS             complement(16047..16928)
                     /locus_tag="VQM60_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET1080024.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(17069..18397)
                     /gene="vncS"
                     /locus_tag="VQM60_000109"
     CDS             complement(17069..18397)
                     /gene="vncS"
                     /locus_tag="VQM60_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET1080025.1"
                     /translation="MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTKNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVVQD"
     gene            complement(18394..19050)
                     /gene="vncR"
                     /locus_tag="VQM60_000110"
     CDS             complement(18394..19050)
                     /gene="vncR"
                     /locus_tag="VQM60_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET1080026.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCIFTVRNVGYKLERK
                     "
     gene            complement(19099..19182)
                     /gene="pep27"
                     /locus_tag="VQM60_000111"
     CDS             complement(19099..19182)
                     /gene="pep27"
                     /locus_tag="VQM60_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET1080027.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(19361..20740)
                     /gene="vex3"
                     /locus_tag="VQM60_000112"
     CDS             complement(19361..20740)
                     /gene="vex3"
                     /locus_tag="VQM60_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET1080028.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(20792..21439)
                     /gene="vex2"
                     /locus_tag="VQM60_000113"
     CDS             complement(20792..21439)
                     /gene="vex2"
                     /locus_tag="VQM60_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET1080029.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(21452..22729)
                     /locus_tag="VQM60_000114"
     CDS             complement(21452..22729)
                     /locus_tag="VQM60_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1080030.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYDSSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(23086..23256)
                     /locus_tag="VQM60_000115"
                     /pseudo
     CDS             complement(23086..23256)
                     /locus_tag="VQM60_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(23399..23998)
                     /locus_tag="VQM60_000116"
     CDS             complement(23399..23998)
                     /locus_tag="VQM60_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080031.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            complement(24135..25019)
                     /locus_tag="VQM60_000117"
     CDS             complement(24135..25019)
                     /locus_tag="VQM60_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET1080032.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(25023..25409)
                     /locus_tag="VQM60_000118"
     CDS             complement(25023..25409)
                     /locus_tag="VQM60_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET1080033.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(25402..26745)
                     /locus_tag="VQM60_000119"
     CDS             complement(25402..26745)
                     /locus_tag="VQM60_000119"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET1080034.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(26832..27716)
                     /locus_tag="VQM60_000120"
     CDS             complement(26832..27716)
                     /locus_tag="VQM60_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1080035.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEILLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSSDT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(27728..28345)
                     /gene="cysE"
                     /locus_tag="VQM60_000121"
     CDS             complement(27728..28345)
                     /gene="cysE"
                     /locus_tag="VQM60_000121"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET1080036.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHSFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHAVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(28361..30574)
                     /gene="pnp"
                     /locus_tag="VQM60_000122"
     CDS             complement(28361..30574)
                     /gene="pnp"
                     /locus_tag="VQM60_000122"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET1080037.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(31259..32125)
                     /gene="metF"
                     /locus_tag="VQM60_000123"
     CDS             complement(31259..32125)
                     /gene="metF"
                     /locus_tag="VQM60_000123"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET1080038.1"
                     /translation="MSCQTPSLSFEVFPPNPAVGNDNIILALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIELIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNEHFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(32189..34438)
                     /gene="metE"
                     /locus_tag="VQM60_000124"
     CDS             complement(32189..34438)
                     /gene="metE"
                     /locus_tag="VQM60_000124"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET1080039.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(34699..34965)
                     /locus_tag="VQM60_000125"
     CDS             complement(34699..34965)
                     /locus_tag="VQM60_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1080040.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            complement(35088..35494)
                     /locus_tag="VQM60_000126"
                     /pseudo
     CDS             complement(35088..35494)
                     /locus_tag="VQM60_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            35641..36642
                     /locus_tag="VQM60_000127"
     CDS             35641..36642
                     /locus_tag="VQM60_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET1080041.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMASILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVRDLDIVSFQNSDHRCIFTEITF"
     gene            complement(36710..39115)
                     /locus_tag="VQM60_000128"
     CDS             complement(36710..39115)
                     /locus_tag="VQM60_000128"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET1080042.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVESVESPAADLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENTVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIGTDK
                     APYYAARILDNVIIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPREELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQVSLEEKVNT
                     EVR"
     gene            complement(39192..39701)
                     /locus_tag="VQM60_000129"
     CDS             complement(39192..39701)
                     /locus_tag="VQM60_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1080043.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GIGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(39701..40747)
                     /gene="pheS"
                     /locus_tag="VQM60_000130"
     CDS             complement(39701..40747)
                     /gene="pheS"
                     /locus_tag="VQM60_000130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET1080044.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
BASE COUNT        11589 a         9408 c         7671 g        12418 t
ORIGIN      
        1 ttgagtataa gatatattaa aagcgatggg atcagccaat cgctttttta gtttgcattg
       61 gttattttag gaaactctta caaaaaatca aaaaaattct tcacatcctc tacataccta
      121 tgcttttcta ttaagcttgc taatagttcc agattgtgcc tctgatagtt atcttcacga
      181 cgtagttctt catacatttc gataaaggga aaacccttgg atttggcaaa ttctaactca
      241 gcctcgtaat cataagcgac tttacgaaat aggatattta ccaattcccc atcttcaacc
      301 tctatcacgg catactgggc acggtgattt tttaacgcct cccaattaaa atagggcatg
      361 ccaatcgacc ctggattgat gatttgttgc ccttgactac cataacgaag caactacttg
      421 tgaacatgac cataaactgc cacgtccgtt tccgcatcta gcagttggtc aaatttctct
      481 gtatcattct caactagcaa gtcaccacca tagtttttgt caggtaaatt atgagagata
      541 gaaaagcgca atccgtcaat ttctttcttt tccagcaaag gcaagcttcg tagccagaca
      601 atcgttgcag gatccattcg ctccatcaaa tactgtgtca tacgcaagag ctgaacttcc
      661 tgtgggtctt ctaagccata ttgcccatct aaagcctcaa ggacacaatc atcccaattg
      721 cctcgaacac ttgccgtgat aggaagttcc tttaacagag cgaccaagtc atttgcacct
      781 ggaccaggga ggaaaatgtc tcccatgagc caatattcac tggctccttg agttttagca
      841 tctgcaatca ctgcttctag cgccgtcgca ttgccatgaa catctgataa aattgcgatt
      901 ttatggttta ttgtggtttc cttccgtttg gtaatctact ctttcttccg ccacttgaag
      961 caagatttct gtttcctgcg ggttcaactt ggtttgcaca gcctctgcta ctgctcttgg
     1021 tactccaact tcgacaatct catccacact ggcttccttg attttggtca gagacttgaa
     1081 gtgcctcatg agattttgct tgcgtttagg tcccagacca tcaatcccat ccaattgaga
     1141 tgagaaagaa tttttggagc gcagttggcg gtggaaggta atggcaaagc ggtggacctc
     1201 atcttgaatg cgttggagga ggaaaaattc ctgagaattg cgagacaagt ccaccacttc
     1261 cagcggatct ccaaagagca attcatgggt ttggtgctta tcattctttt gcagacctgc
     1321 aattggaata tccaagccca gttcttcttg gatgacttgc ttagcaatat tgacttgacc
     1381 ttgcccccca tcaatcacaa tcaaatctgg aggagtcaaa gcctcacgct gtactcgacc
     1441 atagcgtctg cgaatgacct ctcgcatact ggcatagtcg tctggcccga ctacggtctt
     1501 gattttatac ttacggtaat ccttcttact aggcttgccg ttgacaaaga ccaccatggc
     1561 tgaaacagga ctagttccca tgatgttgga gttatcgaag gactcgatgc gaactggggt
     1621 tgggatttga agcaagcgtc ctagattttc aatagctcct tgggtcttct cgacagattt
     1681 ctctagcagg ttgaacttct gctctaggct aacacgggca ttttttatag ctaaattgac
     1741 cagctgcttt ttctctccac gttgaggctt aagaatcttg gaatccacca aggccttgac
     1801 agcctcttcg tcaatatctt gcggaatcag tacctcattg ggaactagat gggatttttc
     1861 ttgatagaat tgtcctacat aggtcaaaaa atcctcatct ggatcattga agtaggggaa
     1921 gagattgaca tcgcgctcga tgagcttacc ctgacggaca aagaaaacct gcacacacat
     1981 ccagccctta tccacatagt agccaaagac atcgcgattt tggagatcct tagccatgac
     2041 ccgttgcttg gttcgaagcg ttccaatagc ctgaatcagg tcacggtatt ccgccgcacg
     2101 ttcaaactcc atagtctggg ctgccgctgc cattttgccc tttagctcat caatgatttt
     2161 gttatcctgc cctttcagaa aatcagaaac ctcctgggcc atagacttga aataaatctc
     2221 atccttctta cagatggtgt gggccatgca ttgaccgata tggtaataaa aacagacctt
     2281 agagggcggg ttggtacact tacgaaaagg gaatatccga tccagcaacc gcttgatttc
     2341 attggctgcc cccacatcgg gatagggtcc aaaataaaga cctccgtcct ttttgacctg
     2401 acgagtgata atcaagcgtg gatagcgctc attggtgatt ttgatgaaag gataggactt
     2461 gtcatccttg agcatgatat tgtacttggg cttgttttcc ttgatcaggt tgatttctag
     2521 gagaagtgcc tcaatattag actccgtaac aataaattca aaatccacaa tttcagacac
     2581 cagagcctct gtcttggtat catgacttcc acgaaaatag gaccgtactc gattacgcag
     2641 atttttagcc tttcctacat agataatggt gccattttta tccttatgaa tgtagcaacc
     2701 agggctggtc ggcaagagct ctagttttga tttaatcaag ttgttcatag ttccattata
     2761 gcaaaaaaag tagggaaaga tagtacccta ctcactagtc tcatatattt ttcggaggtt
     2821 tgcaacatcc tcttcctgga taccataaaa ggaacctgct ggttgcatga cagacttctc
     2881 atctgtatgg tccaagccaa tcgcatgacc taactcatgt tctgccgtat ggacaaggcg
     2941 ttcataggag tagccatagt atggattgga caaataataa tgattcaacc gcaccgttac
     3001 ggacaagaat tgccctgtta agagattagt ttgactttcc gcctctcctg ccacaggagt
     3061 gcctccgtcg ttcatctccg tagccgtaat atccgcctta ctagactcag tcacgagttc
     3121 aaagttaaaa gcaccagttt gattccagtt ctggattgct tctaaataag cctcttgaaa
     3181 gcgtgaatcc atctgcggat ccaggtaaat acgagcagag gcctgtgccc accttccttc
     3241 agaatgctgg tgactatctg tctggcttaa cttaggcagt tgccctgttt tctcccattg
     3301 gtcaatactt tgttgaccaa tttttactga ccgttctgct tgctttagcg ctccttgaaa
     3361 atctccgttc agataccaga gaagtccgaa agcaagcaca cataagagca caactatcca
     3421 aaccagacgc caaaacaaac gccacacaaa agaaaagaaa gcccctatca aacgaaaaag
     3481 ccagcgcatg tctctccacc tttctagcaa ataagaatta ttttactaaa agaagctaaa
     3541 agaaatctaa atactgactt tttcatctta acgtcctact atttttttga gtaactggat
     3601 agctttgtat tttaaaggag atggatggta acggaaagta cctgctttgc gtacaatata
     3661 gccattaaaa ttctgtttaa aacgcaaaac accatcactt ccatcaaaaa tcccttgaat
     3721 gcctaggaag ttgtatttag gtattccacg ttttatgctt tccaacataa catatttttg
     3781 aagcagggca ggggcataga acttattaaa ctcagtgtag gaaccactaa agagataagt
     3841 cgtttcctga ggcatataaa caaataaact cccagctaaa acaatatctt cttctccata
     3901 tttttcaatc aagtctcgcg cttctgcttt tcgaacttca aacgtttcaa attgactaga
     3961 atattctctc agttgatttt gttttttctc agaatgagga tttttactca aatcaagtcg
     4021 caacttgtcc aagttttctt ctagtttact ttgttcacct tgcaatttgc tcatatagtc
     4081 cgaaaaattt aagcttgcta tgagaaactc cgcttgttct ccaaaagtat cataaaaatg
     4141 ctcataatat tctaaacttt tatcactata ttctctacgt tcagaggttt cttttgttat
     4201 attcttaaaa atcgatagtt cttcacgttt taactttttc aaccgaatgc caaaggtttc
     4261 agcctttttc accaagggtt tacccttttt gctaaaactt ttaagcaaac tcttttcagt
     4321 taattcagtt aaatctttat agtataacca atctggttct ccacctgggt aacctgttgt
     4381 taagccatca aattgataac ctaaatcagt caaaccttga ataatacttt ttttctcagc
     4441 atctattgga ttaccttggc tatcaaaagt ttgataagtt tcataaggtt ttacaagcaa
     4501 ctctaataca ccattttgct tggcatattc ttttaactct gcataaaaaa ctggaagagc
     4561 atcttgttgg gtataaattg gccccgaatt gagttccata tgcagaccac ccagcatggg
     4621 caagctataa accagagctg caacttgaat ttctccttct tgtttcaaag caagataaac
     4681 aattcgagcc cctctttttt ctagcaaatc ccccatctgg acagattgca taaaggaacg
     4741 agaagaaacc tgatcagaat aagtctgaaa ctcttctttc gtgagtgttg ttagtgccat
     4801 atacttactt tctatgtttt tttcttaatg ttttacggaa atcaagagca agtcttaaca
     4861 gaggatagag aggatgagtg ggcattgtaa attcacccaa gtattcttca atcgttggat
     4921 taaatttttc cttaaaatga taaagtccac cattgagaga gttttcaaca ccacctaaat
     4981 tttgccagac catacctcgc tcaaaggcat agcgagccgt ttcataccat gttaaaattg
     5041 gtgcattgta acgtttaaaa tcatcatcca taccagcata tatattaaca gaggtagtac
     5101 caaattccaa actcaaagta gccgctaaag gaactctcgc ttgacctaca tctatatatt
     5161 cctgcaagaa ggtcaattcc tctaacaaac gttctttttc cttcttctgc gcttctactt
     5221 ttgaagttcg agtcgactca gtaaaggtct cttccaaggc tctatttttc gctaactgtt
     5281 cttctaattc ttgcgaacgt ttagaaacat ccaaggtggc caaggtgata taggccttgt
     5341 ccttaaaatt atctaacaat tttttataat aggcttcatt cctcaaatga atctcttttc
     5401 gcttctcagt ttttttcatc aactccgaaa atgaatctaa tagttccagt ccaccatatt
     5461 gaatctcaag ccctttgttt cgtgctgttc gaatagcctg ttttgttgac ttggaaagtt
     5521 tatcttcttc aaaattttcc ttgtatattt tcgcctgaat acgaggttga atggtgtctc
     5581 ccatttcctc cgtttttcct gaccacctta ctcccatttg ttgcaaacta tcaataatag
     5641 ccagattttc aggaaattct gtcttttcct gattgattaa actttgagat aggcaaatac
     5701 ttgggtcaaa agtcacaaaa accgctctct tactgcgagc ataggactta atagactgaa
     5761 tggcaaaatt caagagttct ttatccccat aatccaatat aggtcctctt gggatgtaaa
     5821 acattttata gcctagcgga agagttctaa tcaaaatact agctgtcgcc agtaattttt
     5881 cttccctgta aacaccaaac ttctcatgtt gccaattaga cttaacttcc tcccaagcac
     5941 tactttgtaa tacattggct aattcatgtt ctttgacaaa ctgatcatat tctaatgtgg
     6001 gaatgccaat ttgataacgg tacatttcct actctctttc ttccagtatt ttattactat
     6061 tctactactt tcgtttgaaa agtctagtga aaacatattt tattttagtt ctactccccc
     6121 tatgctgaag ttcaaactaa gtcagtctct cttctaattt gaaatcaatt ggtagggttg
     6181 ttaaaaaaac ttccaattgt ttttcccagt agtaccactc gtgagttcca gcactatggc
     6241 tataggtcac atcaaaacct agttttttga gatttttcac tgcgagatta ttggcttcgt
     6301 acaagaaatc ctgttcgcca caccacgccc aaagtttggt ctttttatcc gattttttag
     6361 ccagactttc aagagaatag ggactagttg tccagtctct aatctctcca aaaacacctc
     6421 tccagtaggc tggacttccc agattttgac tttcaggaga aaagttttga aagctgaggg
     6481 cacctgaaaa actagctgca tgagaaaaac gatttgtcgt aagagccagt ttgaagcagc
     6541 cgtagcctcc catagaaaga ccagcgataa aggtcttttc acgcttgcta gtcatattag
     6601 ggaagaagcg tttcagaacc tgtggcaatt cctctgctag agccatgtag tagtcaaaac
     6661 catactgggt atcggtgtac caaccattgc tggtattggg cataacaacg atgagattag
     6721 ttcctcgaag caagcgttct acattggtcc gcttaagcca actattatga tttccagaca
     6781 tcccgtgcaa aaggtacaag acgggaatat cttcacactc tggttcttcc actcgattgg
     6841 catcagggta gaggacattc accccccact ccatatccaa tacttgtgag taatactcga
     6901 ttttcattac tgccatgatt ttctccttct atttttctat aagaaaaagc cgaaactcga
     6961 ctttctctct gtttatttca acgattattt tgcttccatg actactggta aaatagctgg
     7021 acgacgcttg gtttggtcaa agagatactt ggtcaaatta tctcgaacct tccctttaag
     7081 atctgcccag tcaaagtcat ctccttgaag atactcttct accgtttggt taatcaattc
     7141 tgaactttca cggagaatat cgcgactctt cttgagataa acaaatccgc gcgtatgaac
     7201 acgagcccta gccacaattt tcttctcacg acggttgact gtaatagcca cgatgaaaat
     7261 tccatcctct gacaagacct tacggtcacg aagaacaaca tttccaacat caccaatggc
     7321 attcccatca atcaagatat ctcctgctga aaccgatcca gctggaacaa agtctccatt
     7381 ctcgtaagcc atggtcgtcc cctttttagg aatgaagatg cgttctggca acatcccaac
     7441 tgccatggca gccttagcgt gagcatccaa ctcacgatac tccccttgga cagggaagag
     7501 gtacttaggt tgcaaaagat tgatcatcag ctgcaaatca cgcacatttc cgtgccctga
     7561 tacatgtaaa ctttgggtaa tcaatttgac aaccccacct gcctgataaa tcatattttc
     7621 cacacgcgca acaaaggctt ctttagcaat agacggagcc gtagcaatat agaccaggtc
     7681 cccatccttg atttctacat aacgatggcg accaatcgac atcttacgaa gtccattgat
     7741 aggttcaccc atacgacctg tctcaagaat aatcaactca tggtcttcaa agcgagacat
     7801 atctttaggc ttaatcaaga gaatttcgtt ggctaaagac aacttcttaa gacgaatcgc
     7861 tgtgcggacg atattttcaa tatcaaatcc tgtcaagacg atacgtcgac ctgttttatc
     7921 cgcagcgtca aaaatctgct gaatacgaga aagattactg gaaacagctg caacgatgat
     7981 acgaccttcc cagtcagcaa tagtttgggt aatttcatcc ctaacttcac tttcactagc
     8041 cacctgaata ttgctgtctg cattggccga atcactgagg agagccagga cgccgtcacg
     8101 accaatctct gccaaacgag caaaatcagt tgcataagat tcactagccg tttggtcaaa
     8161 tttgaagtca cctgtataaa cgatgcttcc ttccgatgtc ttcaagacaa ttcccagact
     8221 ctctggaacg gagtaagtcg tagggaagaa ggaaaccact gtcccaccaa aatcaatctc
     8281 cgtattctca tcaatgacat ggaaatcatt aaatttctta acggcatcat ttcctttgac
     8341 aaagagcttt gccaactcaa tggtcaactc agacccaaat acaggaactt tagcctctgc
     8401 caagagataa ggtagagcac caatggcatc cgcatgcccg tgggtcaaga aaaccccagc
     8461 aatacggtcg ctattttcaa aaaggtaatc catgtttgga atcaccacat cgacccctaa
     8521 ttgttcattt tcaggatatt ttaaccctac attcaaaaca aaaatggact ctccaatttc
     8581 agcaatgtac atattttttc cattctcacg cacaccacca agtgttgtta aactgatatt
     8641 actcattttt cctccaaaat cttagtttat cttgattata gggcaactct accctcttat
     8701 tctaaaagtt ctgatacttt cagccgaatc ttttcctacc attataccat ttttttgatg
     8761 attttcaaaa tgaaggtctg ttttcaaaaa agagttgatt tcccaactct ttcctaactt
     8821 ctactctttt tccatcaaaa aatcataaat ttcttgcacc gtacccagcg ccatcatttc
     8881 ttggtcttta acggtttgtt tgagattttg gtaaatttga ctttctccta tctcccgctt
     8941 tggcgcctct ttattgactg tcatcacacc atcttttatc gtcacaaagc ctagttcttc
     9001 aaatacttga atcatcttga ccagcaagat ttgttgaata ttaagatatg cagccaaatc
     9061 tttcagcttg tagcgaatat caaactctgg gaactggtaa atagtcttgt acaatttagc
     9121 aaactgatct ctagtcccat aacctgtcag ataataggtc ttgtcaatat catttttgaa
     9181 atagacagca gagaaatgct gttcctgaaa aatggtcttc agctgagtaa tatcctctgg
     9241 aatgtttttt acgacaacag cttcactagc cgcaagattt ggcagttctc caggaaaatc
     9301 caagactgga acaccttctg gcaagactgc attttttcca cgaatgttaa aaagttgaac
     9361 accttccact cgcgcatcca ccatcatcaa ctggagggca gtttggccat tccattggtt
     9421 gacagacaat ttaaccgcta actctagatt cttggtttga gaaaactctg tcgcccatct
     9481 gccttgacca aaggctacca cttcaaaact cgcctcaccc ttggaaattt tcagctttag
     9541 atgggcatta cctgccccca tagtacgagc actttcgacc tgaaaattct tgatataaaa
     9601 aataggtttc tgattatcca ttccaaaagg agctaaacgt tcaaaacttt tgaccgtttc
     9661 caagctaagt gcctccaaat ccaactcttc atctaggttt aacttattct tgccaccagc
     9721 atctgcacct ttttcacgaa cataatcttc caaaacctga gataaatctg agagttgctc
     9781 aacttccagc gtcatacccg ctgcacctgc atgacctcca aaggcgatga agaggtctcg
     9841 atggggatcc agagcttcaa aaatatcgac cgcttccaca ctacgagcac tgcccttggc
     9901 acgaccgtct tctatattaa gaacaatgac tgtctgtccc aattcttcca ataaacgacc
     9961 agccacgatt cctagaaccc caggattcca gccttccttg gccaagacct gaaccttctt
    10021 ctcaggatcc accatggtct tggcttcttc atagatagac tgaacgattt ccttgcgctc
    10081 ttcgtttttc tggtgaatca taagggcaat ctcatgcgct tcctcatcat caaatccagt
    10141 caacaaatca atggcaggat tgggatcatc caagcggccc aaggcattca aacgaggagc
    10201 aatctggaaa ccaaccgttt cttctgttac ttcgttggca gcaatcccag ccatgtccag
    10261 catttcttgc agaccaatgc gctgggtatg acccaacatt tccagaccat attgaactaa
    10321 gatacgattt tcatccgtca gactcaccat atctgcaata gttccaatag cgaccaaatc
    10381 aagcaattcc acttgcactt cttctaacag ggcacaagcc aacttgaaag caactccaca
    10441 accagccaaa tatttaaaag gataatccgc atctggatgt tcaggatgga caatagcata
    10501 agcatctggc agggtttcag gcatggaatg atggtctgtc acaatgacat ctactcccat
    10561 agactgagcc aatgcaatag cctcatgacc agcaacccca ttgtccaccg tcacaatcaa
    10621 ggaaatccct tcttgctcga taaagtattt ataaacacta gcattagggc catagccatc
    10681 ggtaaaacga tttggcaggt aaactcggca ctcagcacca agttgttcca aactttcctt
    10741 cacaatagaa gccgaagtca tgccatccgc atcgtagtct ccataaacga gaatattttc
    10801 cctttcttca atagcctgac gaatccgctc cactgccttg tccatatcat ggagcagata
    10861 agcatcatgt aagtcctcca aggaaggttc taaaaacttc ttcagacttt cttggtcctg
    10921 aatccctctc tcaaacaata accgagccac ctcaggaccc agtccagcct tcttggctat
    10981 ctttgtaaaa tccgcatctt ctacctgcag ggcaaactgc cattcataag taggtgttat
    11041 caaaaagaca tccactcttt ctaagaattc tattgcttca ttataacatg atttagacgt
    11101 tttctctatc cagattgctt ttttataaca ttttatagta tattgaaact agaatagtac
    11161 acctctactt ctaaaatatt gttagaaatc gatttgactg tcccgatcga tttttcctgt
    11221 tcttatttca ctatagtgaa ttttttcaga tacgatttga caagaacatt cttttattgc
    11281 agaatcgtgt tataaaatct aacataaagg agttctctca tggctttagt agaatttaaa
    11341 aacgtcgaaa aatattacgg agactaccac gcactccgca acatcaatct ccgttttgaa
    11401 aaaggacaag ttgttgtcct gcttggacct tctggctctg ggaagtccac tcttatccgt
    11461 acgatcaatg gtttagaggc tgttgacaaa ggaagtctcc tagtcaatgg gcaccaagtt
    11521 gctggtgcca gccagaaaga tttggtacct cttcgcaagg aagtcggcat ggtttttcaa
    11581 cattttaacc tttatccaca caaaacagtg ttagaaaacg tgacacttgc acctattaaa
    11641 gttctaggaa ttgataaaaa agaagctgaa aaaaccgcca aaaaatatct ggaatttgta
    11701 aatatgtggg acaagaaaga ttcctatccc gccatgctat ctggtggaca aaaacagcgg
    11761 atcgccatcg ctcgtggtct tgctatgcat ccggaactcc tcctctttga tgaaccaaca
    11821 tctgctcttg atcctgagac tatcggagat gttctagcag ttatgcagaa actggcgcgt
    11881 gatgggatga acatgatcat cgttacccac gaaatgggct ttgctcgaga ggttgcggac
    11941 cgcattatct ttatggccga cggagaagtt ttagtagata cgacagatgt cgataacttt
    12001 tttgacaatc caagcgaacc tcgtgcccaa caattcctca gcaaaattat caaccacgaa
    12061 agtgacaaag tcaaataagg aggcgcctat gaaaaaaaat tctttttatc agcattattg
    12121 attagccttt tcggccttgc tgctgccaaa ccagtccagg ctgatacaag tatcgcagac
    12181 attcaaaaaa gaggcgaact ggttgtcggt gtcaaacaag acgttcccaa ttttggttac
    12241 aaagatccca agaccggtac ttattctggt atcgaaaccg acttggccaa gatggtagct
    12301 gatgaactca aggtcaagat tcgctatgtg ccggttacag cacaaacccg cggccccctt
    12361 ctagacaatg aacaggtcga tatggatatc gcgaccttta ccatcacgga cgaacgcaaa
    12421 aaactctaca actttaccag tccctactac acagacgctt ctggattttt ggtcaataaa
    12481 tctgccaaaa tcaaaaagat tgaggaccta aacagcaaaa ccatcggagt cgcccaaggc
    12541 tccatcaccc aatgcctgat tactgaactg ggcaaaaaga aaggtctaac ctttaaattt
    12601 gtcgaacttg gttcctaccc agaattgatt acttcccttc acgctcatcg tatcgatgcc
    12661 ttttccgttg accgctctat tctgtctggc tacactagta aacgcacaga actactagat
    12721 gatagtttca agccatctga ctacggtatt gttaccaaga aatcaaatac agagctcaac
    12781 gactatcttg ataacttggt tactaaatgg agcaaggatg gtagtttgca gaaactttat
    12841 gaccgttaca agctcaaacc atctagccat actgcagatt aaggaggaca ccccatgaca
    12901 gatttatcat cttggacagc ctattttcag gattttggac aatttttcaa tggtttcctc
    12961 tttactcttg ccctagcgat tggattcttt atcctcgcca tggtcttagg aatcttcttt
    13021 ggtgccatgt caaccagtaa acgtccaatt ttacgcattt tagctcacat ctttgtggaa
    13081 ttttaccaaa acactcccct cttggtgcag tttgttattg ttttttatgg cctacctctt
    13141 atcagtgacc atactatcat gattccaatt tattggacag ctgttctctg cgtgggactc
    13201 tatcacggcg cttatatcgc tgaggttatt cgttcaggga ttcagtctat tcctagtggt
    13261 cagatggagg ccgccttgtc gcaaggtttt acctatatca gtgccatgcg cttgattacc
    13321 ttgcctcagg ccttccgcat cattctccct ccattgacca accaaattgt taacctcatc
    13381 aagaacacat ctactgtagc tatcatctct ggagtagact tgatgtttgt gactaagtct
    13441 tggtcggctc tcaacggaaa ctatatccca gcctttttag gtgctgctct tctctacttt
    13501 gccctatgct tccctgttgc ccagtttggt cgcaagatgg agcaagccaa taaaaaagcc
    13561 tattcacttt aggaggttac tatggaatct attttagaaa ttttaacccc agataaccta
    13621 gtctttatct ttaaaggatt tggcttgacc ctctatattt ctctgattgc catcatcctc
    13681 tctactatca tcggtaccgt gcttgccgtc atgcgaaatg gaaaaaatcc tatcttacgc
    13741 attatttcca gtatttatat cgagtttgtg cacaacgttc ccaaccttct ctggattttt
    13801 actatctttt tggtgttcaa aatgaagtcc acaccagcag gtattacagc ctttactctc
    13861 tttacatcag cagccttggc tgagattatt cgaggcggtc tcaatgccgt agacaaggga
    13921 caatacaaag caggaatgtc acaaggcttc acctcagccc aaatcctcta ctacatcatt
    13981 ctcccacaag ccagccgcaa aatgctacca gccatcattt ctcagtttgt taccgtgatt
    14041 aaggatacca gtctcctcta ctctgttatc gccctacaag aactctttgg agccagccaa
    14101 attctcatgg gccgttattt cgaaccagag caggtcttca gtctttacat cctgattgcc
    14161 ctcatctact tcagctttaa cctagcaatt tctagcctgt ctcatatgct agccaaacgt
    14221 tggcaacaag ctgcagaata atactcttcg aaaatctctt caaaccacgt cagcttcacc
    14281 ttgccgtata tatgttactg acttcgtcag ttctatccac aacctcaaaa cggtgttttg
    14341 agcagcctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat aaacagccac
    14401 tctccagttc aattggagag ttttttgatg agagtagata tttatagacc tcaatttgac
    14461 accatacatt ttctatgata aaatgtaaca aattcattta caacaaatcg aaatctaaat
    14521 gaaatggaat tttctatgaa atcagtcaaa ggactactct ttatcatagc tagttttatc
    14581 ttgactcttt tgacttggat gaacacttct ccccaattca tgattccagg actagcttta
    14641 acaagcctat ctctgacttt tatcctagcc actcgtctcc cactactaga aagctggttt
    14701 cacggtttgg agaaggtcta caccgtccac aaattcacag cctttctctc catcatccta
    14761 ctaatctttc ataactttag tatgggcggt ttgtggggct ctcgcttagc tgctcagttt
    14821 ggcaatcttg ccatctatat ctttgccagc atcatccttg tcgcctattt aggcaaatac
    14881 atccaatacg aagcttggcg atggattcac cgcttggttt acctagccta tattttagga
    14941 ctctttcaca tctacatgat aatgggcaat cgtctcctta catttaatct tctaagtttt
    15001 cttgttggta gctatgccct tttaggctta ctagctggtt tttatatcat ttttctatat
    15061 caaaagattt cctcccccta tctagggaaa attacccatc tcaaacgctt aaatcacgat
    15121 actagagaaa ttcaaatcca tctttgcaga cctttcaact atcaatcagg acaatttgcc
    15181 tttctaaaga ttttccaaga aggctttgaa agtgctccgc atcccttttc tatctcagga
    15241 ggtcatggtc aaactcttta ctttactgtt aaaaattcag gcgaccatac caataatatc
    15301 tatgataatc ttcaagccgg cagcaaagta accctagaca gagcttacgg acacatgatc
    15361 atagaagaag gacgagaaaa tcaggtttgg attgctggag gtattgggat cacccccttc
    15421 atctcttaca tccgtgaaca tcctatttta gataaacagg ttcacttcta ctatagcttc
    15481 cgtggagatg aaaatgcagt ctacctagat ttactccgta actatgctca gaaaaatcct
    15541 aattttgaac tccatctaat cgacagtacg aaagacggct atcttaattt tgaacaaaaa
    15601 gaagtgcccg aacatgcaac cgtctatatg tgtggtccta tttctatgat gaaggcactt
    15661 gccaaacaga ttaagaaaca aaatccaaaa acagagctta tttacgaagg attcaagttc
    15721 aaataagtaa tcatactctc ctgggatttc ccaggagagt tttttatagc caaatgaaat
    15781 aagaccggaa cgatggaatt ttggcattca aatcaattga taagaatatt ttatagtgga
    15841 gtgaaatagg atacgaacaa attgattagg aaaatcaaat gaatttatag aaatctttta
    15901 gcagttatgt tatcctattc tagtttcaaa acgctataga agcagcattg tgctagtcta
    15961 gattcagttt actatactaa aacgagtagc ttgaaatcaa aaaacccacc ctcacaggca
    16021 ggttttatct gtattattca gctagattat gctttacctt ctgaaccgaa tacgtcgata
    16081 cgttcttcaa ccgatgcttg gatagctttt acaccgtcag ccaagaattt acgtgggtcg
    16141 aagagttttt tcttgtcgta ttctgcttcg tttgcttcgt aatcacgagc aaatttacga
    16201 gttgcgttag cgaatgcgat ttggcattct gtgttaacgt taactttggc aacaccaagt
    16261 ttgatagctg cttggatttg ctcatcagga atacctgatc caccgtgcaa tacgattggg
    16321 aatcctggaa gagcttctgt caatttttgc aagtggtcaa ggtcaagacc ttcccagttt
    16381 actgggtaag ggccgtggat gttaccgata ccagctgcca agaagtcgat accagtttca
    16441 accattgctt tagcgtcttc gattggagcc aattcacctt taccgatgat tccgtcttct
    16501 tcaccaccga tagtaccaac ttcagcttct actgagatac ctttagcgtg tgctttttca
    16561 acaacttctt tagccaattt aaggttttct tcaactggaa ggtgtgaacc gtcaaacatg
    16621 attgaagtat aaccaacttc gatacactca agtgcatctt cgtagtgacc gtggtcaagg
    16681 tggatagcta ctggtacagt gatacccatt gattcaacaa ggttagcgat caagttgcga
    16741 gcaactttgt aaccacccat gtatttagca gcacccattg aagtttggat caaaactgga
    16801 gcttttttag cttctgctgc gcgcaagata gcttgagtcc actcaaggtt gtttgtgtta
    16861 aatccaccaa ctgcataacc gttgtcacgg gctgcttgga caaatttttc tgctgaaacg
    16921 attgccattt tatcaggcct cctgtatatt tttatgggtc atcccattta cattgttcat
    16981 tttatcactt tttgccaaaa aaatctagtt tttcccgcag tttcgattga ttttcttcta
    17041 actccatcta tgtaaaccct ttctctccct agtcttggac gacttttgga aaagctataa
    17101 agaaggttaa acgattctcc tccatctcga aacgataagc taatttttca tgttctaata
    17161 gactcttaac cacaaaaagc cccataccag accccttgac cttgcgactg gcattgtcag
    17221 aaaaagactg ggctagtttt tcttgttcct ctgagctaca gctattttcg ataaaaagtt
    17281 ctccttctct ttctccaatt cgaactaagc cacctggaac agagtgctta atggcattgc
    17341 tgatgagatt agaaagaatc aacttcataa ctgatgggtt tagataagcc tgctgatggg
    17401 tcaaactatt gtctatctgg agctctcttt ccttggctag caaggcataa tctttgacca
    17461 gatttttcgt catctggagg aggtcaattg tttccctatc atctcgcaat tcctgcacag
    17521 aagagaggga aagtatctgc agaacatggt gattgagttc atccacaatc cccaaggcaa
    17581 ctcccagata ctggtctcta tccttataac gaccgatatt ctctctcata ttttcgatta
    17641 ggattttcaa actagccagc ggtgttttca attcatgaga agctcctcgt aggaattcga
    17701 ccttcatctt ctccagctgg agaatggctt cattcttttc atgcaagtcc gcaataacag
    17761 tcaagagatg ctggtagagg ctattgattt gttccttgag attacctatc tcatccttag
    17821 aatccacgcg caatcgcact tgggaatcca ggtccatcat ccgacgggtc acccgcttga
    17881 tttccaaaat cggtgcaaca atagtccgag cgtagatgta ggccaccaag agggaaatca
    17941 gaaaggaggc cagcaaggta tagggaagaa actggagact gatttgctcc gcttcctttt
    18001 gtaaatccat ggaagctaga aactggagaa tcatagtacc accgtcttgc gttttcacct
    18061 cgcgctcctc aataaagaga gaggttgtct ggcggtctgt gtccagagga agactgtcct
    18121 tgacttctaa cttgtcctcg gtcatctcac ctttgacggt ccccttgata tcactagtct
    18181 gggaatacaa gtctaacact tgctcgatac tctgcctatc tttcccttct agggactggg
    18241 caatggctgt tgccttttga ccaatggttt cctgacgatg actcagataa gtcgaaggaa
    18301 aaagaaaata aatagctaaa tgaaggcaga taaccagaac actaaatatc gagaaggtat
    18361 agataaatat ctttgtaaat aaacctgttc gtttcatttt cgctccaatt tataaccaac
    18421 attgcgcaca gtgaagatac aatccaagtc tagctttttc cgcaattcct tgatataaac
    18481 atcaataaca cggtcaaagg gaacctcatc tgtcgctttc cagacggcat cgataatctg
    18541 agaccgagtc aaggcccggc cttcattttt cactagatag tccagaattt ccaactcttt
    18601 ggcattgata ggcacttctt gacctgcgag gcttgcactg tagctttcaa agtccacctt
    18661 ggtatccttg taagaaaaga ttcgtcctgt atcgtagtag cgcttgaaaa tcgcgtccac
    18721 cctcactttt aagagggaga gggagaaagg tttttccaga tagccatctg ccaaagaggc
    18781 aaaggcactc atcttgtatt cctcatcctg aaaagctgtc aacatcaaga caggaacctg
    18841 actggtttta cgaatctcag ctaggacttc taagccgtta agcttgggca tctggatatc
    18901 cagtaaaacc agggccacct catagctaga aaattgctcc agagcttcct gaccgtccgc
    18961 tgcctcaata gtttcatagc cacaatccgt caaataatca ctgaccccct cacggatcat
    19021 ctcttcatct tctacaatta aaattttcat actttaactg ctctctattt tttatttttc
    19081 ttagaataaa tacctactct attttctatt atagtctctt gctggccttt tgtctgtaag
    19141 caactgacca ctagataaaa cgttgtgaaa ttcctttctc ataaattcca taactttagt
    19201 atattatatt taagcactaa agtacaaaga aagcaactga aagcaatgat tttcaccact
    19261 gctttcgatt tattttgaat tgttaaatag ctattcctat ccactattct tgaatagaaa
    19321 cacaagatgc aatctttatt ccagactcat tttttcaaat ttattcacca tccagcaaga
    19381 gctcttttgg ttgttttcta aggagattgc ttgaagcaag cgccataacg agaaccacta
    19441 gaaccaaggc aaggacaaaa atgatgataa agtctgatgt ctgaatggaa atgtctaggc
    19501 tcgacaaggt cttgctaaag ccatctactt ctgcaccgcc accaaggtta gaggcttgag
    19561 ccgccttact agcctgtttg gcaacacctg aagtcacatt ggcaaggaca gtgtttccaa
    19621 ttgcacgggc agtgtaatta gctagaaagt aagcagaaac tagagcaggg atagcaatca
    19681 agatagattc ggttatgaat tgacccaaga tacttgcctg cttgaggccg atagagagga
    19741 gaattcccac ttccttgcga cgggcgttga tccaaaggct gagcaagagg gcaaggagga
    19801 gaactgagaa gctcaagcta ccccagaaga ggaggttggc catcttgtac ataccagaga
    19861 tagattgctc aagagctggg tagttagagg agctcttgac gagtgtgtag ctcttccagt
    19921 tgataccact gatgccattc aactctttca taacatcatc caagttcttg tctgctgtta
    19981 caaagaaggt tgcgtcccca taaatggctg tgtcttctgt gtatccataa agttttgcag
    20041 cagtgtgaat gtctgtaata gctgtgtttt cgtaaagttc ttgtgagtag gttactgctg
    20101 acttattatg accatcaaag agtcccttga ttgtcacttc aactgtttcc ttggctcctt
    20161 tttcattatc tgcatcgtag atattagagt ccagtttaac cttgtcccct actttccagc
    20221 cgtgtttggc tgccaagtcc ttgtgcaaga ggattttatc cttgtcgtcg ttggttaagt
    20281 gctctccttc gactagttta taagaaccag agacaaactt gtcttcttta gaggagtcat
    20341 tgacacctgt aatcatcaag ctacttccaa aacgtttggc acgatcagca gtgagattct
    20401 tcttggtttc tggcgtttca atcaggtcat atccagtcaa atctccgata gcgttgatac
    20461 gtttgacata agactcaatg gccttgtttt cggtgatttt tttgatgtct tcacccttga
    20521 tattcccagc accacgaggc gttccttggt tgacgcgacg attgatttgc atggagaagc
    20581 tattggtgat atttttaaag gtctcctgag aagccttggc agtagctccc ttgattgaca
    20641 agccgaccaa actcaagctc gccataagga gaataatcag gaagatgaca atcgatttga
    20701 aaaacttcct tgtaacatag gcaaatgcgt tgtgtaacat agattccctt tctagatttt
    20761 gttttaatca ttctattaaa ataagctcaa attatttact agtattgcgc gtttcagtca
    20821 gtttcttatc ctttaattca agtgtaatat ctgacgcttg tgccacttct ttactgtgag
    20881 ttacgacaat cacacattta cctgttttct gggcaagtga tttgagtagt tcgacaatat
    20941 ctccagcagt tttaggatcc agatttcctg ttggctcatc agctagaata actggagctt
    21001 ctgagaccaa actgcgagca atggcaacac gttgctgttg accacctgat aactggagaa
    21061 cattccgctt gatctggctt tcatccaaac caagctcaag aagtgtatcc ttgcttgcct
    21121 ttttgttgac caagcggata ttttccagcg gagaaagata atctatcaag ttataatttt
    21181 gaaagaccag ggaaatatgg tgcatgcgat ggtaagaata gcccttctta cgaatatcct
    21241 ctccttgaaa aaggatagaa ccttcaacag gactatctag accagcaagt agggacaaga
    21301 gtgtggattt tcctgctcct gactccccaa taatactgta aaattttccg ggttcaaaat
    21361 tataatcgat ctgatatagg actgcttcag cagtattctt ataacggtag gtaacatctt
    21421 gtaattgtaa taaagtcatg atttctcctt cttaactaat agatgataaa atttctttcg
    21481 gtgattttct aaataagaat aggaaacaaa gggctacaga taagcaacta agcagaacta
    21541 gaaaaacata ggattctgca aaagataaga tgctagttga taaactgctt gctttggcta
    21601 gtgtatcttg taagcttgcc tgatctccac ttgctagtag agtttggagt aggtaagttg
    21661 tgattccgtt tcctgcaaca aatgctggaa gcaaagctcc aagagatacc aaaactacct
    21721 ctaaacagaa ttgtaggaag atcgagctct tgccttttcc aagtgcaagt aaaatcccca
    21781 cttcatagac ccgttctctc aaccagagag acaaaaccag aattaaggct ccagctcctg
    21841 ctatcaacat cccataaagg aagatggtca ggaaggtttg gaaagttgca actgagtctt
    21901 tgatttgttc aaaagccttg ttttcctttt cgacttggta gccttgattt tccaaggcca
    21961 agttttctac ctgcttcatg agtccgtcca tttccttagg attttctaca tagaagcgtg
    22021 ctgcactgac ttgagcttca ctattgccca aaagggtttg gctactgtca tagtctgtaa
    22081 agacttgatt ttcactgaag tcagaagaca agcctgtgaa tttctcttgt tttttaccag
    22141 aaaagatgcc gataatctca aactctactg tttgtccttt tccagattca gactgaccag
    22201 catccaagcc aatcttgtca tgaagcgaaa gaccgttctt cttagccaat tcttcgtgga
    22261 taaggatttt cttggaatcc cctttttgaa ggtgtcgccc ttcttttaga ttgaaagccg
    22321 aactggtaaa ggttacatcc ttggatgaat cctcaagagc cgttaagcta accaagttat
    22381 tgtctgcagc tgataaatca tcacgctcca cgctctgctc gccagtcact gcttccttgt
    22441 cttttagttt tgcgaccgtc tcaagttcag gagagacatt ttccagcccc ttaatcttgc
    22501 ttacagatgc taggtctgac aacttgaatg tctgaccatt ctctatcttc ttaatagaaa
    22561 aagatgtatt gagtgattta taaagattgc tttctactgt tttgttggac ttcatcagag
    22621 tcaaacaggc tgaaattccg gccaataaga ccaataaaat cagaaataaa ataaaacttc
    22681 tcagtcgctt tctgctgaca taagcccaag atctttggat tggattcatt tgtcacctcc
    22741 atatttgtaa gactattata aaacccaaat atgaaatgtt tatgaaatac gaaaaaaaaa
    22801 tatcgagtag gggataatct ctagcccctc tcacaccacc atacgtgccg ttcggcatac
    22861 ggcggttcaa ctaactttta acgcatgtcg ttcaaggtaa taatccaaac acgaaaccag
    22921 tccacgtttt tcaaggactg gttttgatat agcacgttta agtactgact tctgagctac
    22981 tatagtagat tgaaactaga atagtacacc tctacttcta aaatattgtt agaaatcgat
    23041 ttgacttccc tgattgattc gtcctattct tatttcattt taatataatt gatagtggtc
    23101 gccccagcca gataccttat ctgctatcca tttaggaacc cctaacttaa gcaatcccca
    23161 taatcgtctc gatttcttct tccattgctt ccagataatc actcgtaggc gagtacgcaa
    23221 gcgctcatct atgctagtga ctatactttt catatttata attcattcct ttcgtttcac
    23281 tcaaggcaca acacagaatg aaaaagtgtt gtgatcttta ttttgtttta taataatagt
    23341 gagaaagcct atcactacta caaatcacgg ggaggtgaat aagtgagtgg tacagccact
    23401 acctcgcata ttttgtcaca tcatttaacg gtacataata agttgtacca tctgaataag
    23461 ttgctacaat atcatttgca tgctctcctt cacctttagc aaaggttgga gctcctgctg
    23521 gatgattttt atttgcctct ttcaattttt caataatggc atttttttct gtatctttta
    23581 tattatcagg atttttcact aagattttgt ctggatatgt cggtttagca gaaacaattt
    23641 ttactgttac ttctttttta ttcgaagcac ttgtccagtt tccagcatta tctttagcat
    23701 ttaattttac agtaattcct gaactaggaa cttcagtagc aggttgatta tcaacattat
    23761 tcaactttaa tttcaaaaga gctgttgcat cagacgtttt atcaatcgtt atatataatg
    23821 atgaattgtt attataacca gttccttcat atttagctgt ttgtgagcta cttgaaacag
    23881 aactgaaatt atacccacta cctccctgat tatcttcaat gcttacgtct aaatgaactt
    23941 ccccactatt atttggctta gcaacaactg ttatagtaaa ataacataaa atttgcataa
    24001 atagattagg gaaatcaaag cagcttctag gaatgtttta gcagtcacag tgtactttcc
    24061 cagcatcaag ccactataac tctgcacata aaaatggaga agatggcaat cctcttctcc
    24121 aaatattaac ttctttacaa accaactata gttgacaaag aacctaaaat caattgataa
    24181 cacaaggtca ggtcggtcaa ctctttcaac tgaagccctg tcaactcttc ccatttatca
    24241 atcttgtatt ggagagaatt gcggtgcaga tagagttgct gggctgtttt agtgagaaca
    24301 gcactatttt cccaaagaga gagaatgatt tcctgaatct gatcttgatc caaaatcatc
    24361 tggtgtagac attctttaat tggcttcaag tccacgagtc tttctcccat actccaaaga
    24421 tagagctgag aaaaagtatg aacaccttgg tgaccctgac gccaccatgt cttgaacaaa
    24481 tcccgctcag ctttgattaa gtctgatagg gcttgatgtc ctgtctgaga ccaaacctga
    24541 cccaacatga tagaaagacg aagtccaaaa tcatactcaa ccgcttcaat cgtatcactt
    24601 aaaatatctc ttacagaagt gtatttgtct tgttgaagca cgaaaacata atcctgagct
    24661 ccgacctgta gcactgtctg acaattcgga aaaagagtcc gcatcatatc tagccaagaa
    24721 gtcagatttt cctgctgaaa ataagaaaga tggcaataaa ccaactgaat ctttttaaaa
    24781 acttgcggtg cctgtccctt gccttcaacc agataggaat accaagggtt tagcgaacga
    24841 gcctgctcct gctgggtcaa aagggcaacc aactgctttt cacgctcgct gagcccagct
    24901 tcctccagca aaatccactg ctgagaagct aaagggagcg tgagatagcc ctctttctct
    24961 actggttggt ctgaaatccg agcctcagga aaccagtctt gtagttcttt ttccctcatg
    25021 ttctagccct ccactttttg gatgcaccat gaaaccaaac tctcaagacg ttccagattc
    25081 tcagtcatat ggagatagcc cataaccgct tcaaatcccg tggacatacg ataagtcacg
    25141 acatctgcat ttttagcctt tgtgtggcta ttggtattgc ggccacgttt gtagatttct
    25201 tcttcttttt ccgttaggac ctgctcctcc aacatgagag caatcaggcg agcctgagcc
    25261 ttggctgaca catacttggt tgcttcttga tggagtttat tgggtttggt catacctttg
    25321 aggatgaggt gacggcgaat atacatagaa tacaccgcat ccccctcaaa ggctagcgca
    25381 atcccgttaa tgagattgac atcaatcacg tgtccacctc actccatcct tggtatcaag
    25441 gagcttaatt ccttgagtaa ccaattggtc acggatttgg tctgctgtcg caaagtcacg
    25501 attggcacgc gcctcttggc gtttttgaat caagtcttca atctctgcat ccaaaacttc
    25561 ctcaacaaag acaattccaa aaatttctaa catatctgca agagcttgct tgacacttgc
    25621 atcatagttc cctgagttga tccatttggc catttcaaag acaactgtga taccgttggc
    25681 agcattaaaa tcttcatcca tagctgctac aaacttatct ttaaagtttt gtaactcttg
    25741 ggcatccacg tttcctgtaa atggttgttc gtaagtattc ttcagatact tgagattggt
    25801 ctcggcatcg cgaactgcct tttccgtgaa gttgataggc ttacggtagt gctgggtcgc
    25861 aaagaagaaa cgaagtactt gcccatcaag agttttaagg gcatcgtgta ccgtaatgaa
    25921 gttacccaag gacttagaca ttttgacatt gtcgatattg acaaagccat tgtgcatcca
    25981 gtagttagca aaagccttgc ctgttttagc ttcagattgg gcaatttcat tggtgtggtg
    26041 tggaaactct aggtcagctc caccaccgtg gatatcaatg gtatcaccta aaatctctgt
    26101 cgacatgact gaacactcaa tatgccaacc cggacgtcca ggtccccaag gactatccca
    26161 agaaatctca cctggtttgg aagatttcca tagagcaaag tctacaggat tttccttacg
    26221 agccgtttct tcatcggtac gacctgaagc acctagctcc aaatcttcca aggttttatt
    26281 agccaattta gcatagttgt gggatttttc tacacggaaa tagacatccc cttgactctc
    26341 ataggcaaag cctttctcga tcaagtcttc cacaaaacgg atgatgtctg ccataaactc
    26401 cactacacgc ggatggcgag tcgcaggttt cacgcccaag gccgtcacat cctcacgaaa
    26461 ggcagcgatg tacttatccg caacctcctg aggcgtgatg ccttcttccc tggcacggtt
    26521 gataatctta tcatccacat ctgtaaaatt ggaaatatag gcaaccttat acccacggta
    26581 ctcaaaatag cgacgaatcg tatcaaaagc taccgtcgaa cgggcgtttc ccacgtggat
    26641 atagttgtac accgttggcc cacaaacata catcttgatc ttgccgtcct caatcgggac
    26701 aaattctcgc aaatcacgag acatggtgtc atagatttta atcataaatc ataatcagga
    26761 aagctaaaat ccaagaactg ttagtttcat cactaaacta agagctcaag taaatttcag
    26821 tccgaatgtc tctacacttc ggaatccctt gctcctttct cattcagata aaccacctgt
    26881 gtctgtttga caaagccaat tttttcatac aaacgtttgg cacctacatt gctatcttcc
    26941 actgcaatct gaaattcctt atcattttgc tcaattagtt ggttgacaag ggattttgct
    27001 aagtagcttc catagccttt cccacgttca ggttctgata ttgctaaacc gtagaagtaa
    27061 ttcgtatcgc tcgataaatc aaccgtgcaa gtcccaataa cctgaccagc ttttaataaa
    27121 atatatagtc ggctttctgg atctttcaga gcttcagcga catatctatc cacaacttct
    27181 ctcgattcat gttcctctga aaatgcctga aattttaatt gactaatttg atcctgatac
    27241 gaactatctg ctaacaaaat ttcaagatgg gaaacatttg ctaacggata aggtcttcta
    27301 tccttaccta accaagtttc tgtctcttca tcctcgatta gtccccagtt actggcaaag
    27361 tcaggatgat tctctaaaaa aatacgttct gtctgaaaag tgactgaccg aatagggaaa
    27421 gaagctgttt ctctctcaaa actagtaaac aatgcacgcg caatcccctg acggcgatga
    27481 cctggatgaa ccagtatcgt cacttctaca tcttggtcat ctgcatagac agttaataaa
    27541 ccaacaagtt tgcctttttc ataataaagg aaaaaggcgg gcatgtttgg gtcaaaatta
    27601 agcatgttag agagataggg atcgcgatag gtaccgtcat agttttggca acagttaatt
    27661 acttttttcg cctcagatag ctcctcttgg cttaacttgt ttcttgcttg aatcatatag
    27721 gtatcctcta caaaccagac gatctgtgac tggcatcttt agcctgctcg agtttattga
    27781 cataatactc tcgtttttct tcgactgcgt gaataacagg ctcatctttc ttaccatgaa
    27841 gacggacaat cttggccgga ataccgacaa ccgtcacatc actaggtaca tctgctacga
    27901 caactgctgc agcaccgacc ttggcatttt caccaatttc cacaggtccg ataacttggg
    27961 catgggctga tatgagggct ccctttcgta cagtcggatg gcgtttgcca cagtctttcc
    28021 ctgttccccc aagagtcact ccgtgataga gaagaacgcc tttttcaaca atcgctgtct
    28081 ctccaatcac cagaccagaa ccatggtcaa taaagacacc tgaatcaatc tgggctcctg
    28141 gatgaatctc aatctgagtc caaaagcgcc aaaactgact gtgcatacga gctaagagtt
    28201 tgaagctgtg cttccagaga aaatgcgaga gacggtgggc cgccaaggcc ttgacacctg
    28261 gataagtcag caaaacctcc aaagtggtgc gggccgctgg atcattttct tttacgatat
    28321 caatggtttc gcgccaccac cccatacatt tctccttttc ttattctgaa tcttttggtg
    28381 tttctgtaaa ttctttctta ggtttgtgat ccttgtgatg acgtgggcgg tgagggcgct
    28441 cagacttttc acctttttca tcatgctcag gttttggcgg acgaggtaga agagccttca
    28501 tagaagcatc gatacggcct ttttcatcaa tcttgataac cttaacatca acttcatccc
    28561 cgatttctac caaatcctct acacgattgg tacgagtcca agccatctca gagatatgaa
    28621 caagggcatc tgtcttatca aagaggttaa caaaggcacc aaatttctcg atacgaacga
    28681 ctttagcacg gtaaacttca tccactttgg cttcacgaac caaaccagca ataatttctt
    28741 tggcacggtt aatagcatct tggtcactag agtagataga cacatttcct tcttcgtcta
    28801 tatcaatctt aacacctgtt tcagcaataa tcttgtcgat ggtttctcca cccttaccga
    28861 tgacaatctt aatcttgtcc acatcaatct tgatcgtatc aattttcgga gcagttggag
    28921 ccaattctgg acgaacttct ggaatggttg cttcaatgac atcaaggatt tcaaaacgcg
    28981 ctttcttggc ttgagcaaga gcctccgtca agatttctgc agtaatccct tgaatcttga
    29041 tatccatttg aagggctgta atcccatcac gagtacctgc aaccttgaag tccatatctc
    29101 caaagtgatc ttccaaacct tggatatctg tcaatactgt gtagttattt ccatctgaga
    29161 taagtcccat agcaatacca gctactggcg ccttgattgg cacaccacca gccataaggg
    29221 caagagttcc cgcacagata gaagcttgag atgaagaacc gtttgattcc aaaacttctg
    29281 ctactagacg gatagcgtat gggaattctt ccaagcttgg caagacttga gcaagagcac
    29341 gctcaccaag agcaccgtga ccgatttcac gacgacctgg cgcaccgtaa cgacctgttt
    29401 cccctacaga atattgaggg aagttatagt ggtgcataaa gcgtttcttg tactctggat
    29461 ccaaaccatc aatgatttga gtttctccca tcggagccaa ggtcaagact gaaagagctt
    29521 gagtttgccc acgagtaaag agacctgaac catgtacacg aggaaggaag tcaacaaccg
    29581 catccaaagg acggatttca tcgaccttac gaccatcagg acgcaccttg tcttctgtaa
    29641 ttaaacgtcg cacttctgcg tgttccattt gttccaagat ttcagccaca tcacgcataa
    29701 tacggtcaaa ttcttcgtgg tccgcatatt tttcttcgta aacggcagtc acttggtctt
    29761 tcactgcttg agttgcagct tcacgggcca atttctcttc tacttgaact gccttttgga
    29821 ggtcactgtt gtaggctgca atgatttcag cttgcaattc agcatccacg tgaagcaatt
    29881 ccacttctgc tttttcttta ccgacagcag caacgatttc ttcttggaag gcaatcaatt
    29941 ctttgacagc ttcgtgccct ttaagaagcg cttccaacat gatttcttct gacaattctt
    30001 tggcaccaga ctctaccatg ttgatagcgt gcttggttcc agctactgtc aattcaagaa
    30061 gagattgctc tgcttgttct tgacttgggt tgatgatgat ttggccatct acatatccca
    30121 cttgtacccc agcaattggt ccgtcaaatg gaatatctga aatagacagt gccaaagatg
    30181 aaccaaacat agcagccatt ggtgcagatg cattttcatc ataagaaagc actgtattga
    30241 tgacttggac ttcattacgg aaaccttccg caaacatagg acgaatcgga cggtcaatca
    30301 aacgcgctgt caaggtcgca tctgttgaag gacgtccttc acgtttcata aagccaccag
    30361 gaaacttccc agccgcatac attttttctt cgtagttgac ttggagtggg aagaaatccc
    30421 cagttgccat tttcttagac ataacggcag cagtcaagac agttgactca ccgtaacgta
    30481 cgacaacaga tccatttgct tgcttagcaa cctgaccagt ctctacaatt aactcacgac
    30541 ccgcaaaagt cgtttgaaac acttgttttg ccattttaat cccctttgga ttgatgaaat
    30601 tatacgcctt gcctacaaag atcaagatac caaggacgtc aaaagcaaag taaaaatagg
    30661 aaactgacga agtcttcgat gaagacaaga cagtttatct tttttacaca gcttttcggc
    30721 cgtgttcaat tacacaagat attttggacg gttcggcttg ccgaacattt ctgtagaaaa
    30781 ataggaaggt gacttcgcac tcgacgagtg ctaggaagct tatctttttt cctaagaaat
    30841 gagaccaaaa ttcaagtcat caagatacca agccgtcaag caactcaaag gaagatagga
    30901 aatcgaacga cggagcgact gctcctaggg agatttatct ttttccacag agttgtaggc
    30961 aagttcagtt ttcaagatac atcattagaa aggtttaata ctaaagtatc taaagctttc
    31021 acgctaatcg ctatcgggcg attagctaaa tgctttacta actctctcgt caaataacat
    31081 cgatttgact cactcgtgtc gttaaatctt acagtttaaa tgcattgtat tatttaatac
    31141 cttcatcttt gtatcaagta cgtacagaat ttattttatc atatttttct taaaaagtga
    31201 ggtctttacc attaaaaagg aaccattccc ctcacctgag aagaatggtt tgcttttatt
    31261 atcctagaga ctggtgatta aacaaggcat gggttgcttg atggatgtat tttgctgtat
    31321 cagcattatt catcgtatag agatgcacac cggcaacatc ctgagttacc aagtctacga
    31381 tttggtccac tgcataggca agtcctgctg ctctgagcga ctcagggtca tgctcatact
    31441 tgtctaagat ggctttaaat ttgcgtggaa gatggatatt ctcacaagtc ttcaagagtc
    31501 ggagagcctg atttcgattc agaattggca taattcctgc atgaatggga acatcaatcc
    31561 cagccaagat gcacttgtcc tgaaaatcat agaagtgctc attgtcaaag aagagctgag
    31621 ttacgaggct cgaacagcct gcatccactt tcttcttaag attttgaata tctgaaatct
    31681 gatttggcga atctggatgc ccttctggat agcaagctcc aataatatca aagtgagggg
    31741 cttgttcctt gataagctca atcaagtcgg ttgcatagcg gaaatccttt tgtggttcca
    31801 cgtctggaat aatatcccca cgaagagcca agattttctg caccccaact ttgtccaagt
    31861 cagcaatagt ttcagcaacc ttgtccttag ttagataaat agctggcaag tgggcaatgg
    31921 tcggaatcgc caagtcattt tggataaagt cagccaaacg aaccgtcgtt tccttgatat
    31981 taaatttatt attgctggca gttacactga taaaatgggg agccaactcc tgcatatcct
    32041 gcaaggctaa aataatgtta tcattaccca cggctgggtt tggagggaac acttcaaatg
    32101 agagtgacgg tgtttggcat gacatatgta ataacctttt ctagttgatt tctttttgaa
    32161 caaccactgt atggagagaa atccaatctt acaatttctc acgcgcagct ttagctgctt
    32221 caacaaggcg gatcaagctt tcttttgttt ctgggatacc acgtgttttc aaaccacagt
    32281 cagggttgat ccaaactttc ttgcttggca ctttagcaag gatggcttcg attgtgttgt
    32341 cgatttcgcc ttcatttggt acacgaggtg agtggatatc gtaaacccca ggtcccactt
    32401 ctgtttggaa gtttttcgct ttgagttcgt ccaagatttc aaggtttgaa cggctggctt
    32461 caaaggaaat aacgtctgca tccatgttat cgatagctgg gatgatatct gtaaattctg
    32521 agtaacacat gtgagtgtgg atttgtgtgt ctgacgctac tgttgagtgt accaagcgga
    32581 aggcaggaat tgcccagtca aggtaatctt cgtaccagtc actacgacgg agtggcaatt
    32641 tttcacgaag agcagcctca tcaatttgga tgattttcac accagcagct tcaaggtcaa
    32701 gcacttcatc cttgatagca agggcgattt gaagagtaga atccttgata gagatgtctt
    32761 cacgtgggaa tgaccagttg aggatggtaa caggtccagt caacatacct ttaacaggtt
    32821 tgtttgtacg actttgtgca tagctagacc atttaacagt gatagggtta agacgagtga
    32881 catcacccca gatgattggt ggttttaccc cacgcatacc gtatgattgt acccatccat
    32941 ttttagagaa gaggtatcct gacaagtttt gaccgaagta ctcaaccatg tcattacgct
    33001 caaattcacc gtgaacaagg acatcaaagt caatatcttc ttgccacttg atccattcgt
    33061 caatcgtttc agcaaggaaa gcgtcgtact ctttttgaga caattcacct ttacggtaag
    33121 ccaaacgttt ggcacgaact tcttttgttt gagggaatga accaatcgtt gttgttggaa
    33181 gagctggaag tttaaaagct tcttcttgga tagcttcacg ttctgcaaag gctggcaaac
    33241 gagtgtagtc tgcgtctgtc aagccagcga tacgcgcacg aagttcagca ttttcaccaa
    33301 cacgctcagt cgcaaagagt tctttgttgg ctgcaagagc ttctgaacct tgaccattgc
    33361 ggatagcatc caagtcacgg atttcatcca atttttcaac tgcaaaggca aagtggttca
    33421 agagtgctgg ttcaaattct tcattagcag ttgtaaatgg cacatgaaga agtgagcaag
    33481 agcttgtcaa aacgatgttt tcagctggga tttgctcaag aacagccaag ctcttttcgt
    33541 agttgttacg ccagatgttt ttaccattga caatacctgc atagagagtc ttgtcagctg
    33601 ggaagccacc tttaacgagt tcaagagttt tcttaccttc aacaaagtca agaccgatag
    33661 catctactgg taagtttaca aggtcagcgt atacgtcacg aacatcaccg aaataagttt
    33721 gaagcaagac ttcaagacct tttttgtcag ccaagagttt gttgtaaagg ttcaagaaga
    33781 gagctttttc ttcagctgtc aagtctttta caagagccgc ttcatccaat tggatgcgag
    33841 tcgcaccaag ttcagccaat ttagcaaaaa cttcttggta agcaactact aagctatcta
    33901 cgaagtcgtc tgctttcacg ccttcttcaa agtctgacaa ttgaaggaaa gtgaagggac
    33961 ctacaagaac aggacgagtg ttcaatccaa gttcttttgc ttcttggaac tcatcgaaaa
    34021 tcttgtgacc agccaatttt acttgagtgt ctttttcaaa tttaggaacg atgtagtggt
    34081 agttagtgtt gaaccatttc ttcattggaa gggcgcgaac gtcccctttt tctccctggt
    34141 aaccacgtcc caaagcgaag tagcgctcaa ggtcagacaa gtccaagttt tgaacggatg
    34201 caggcaccac gttgaaaagg aaagccgcat ctaggaagtt atcatagtga gaaaagtcat
    34261 ttgatggaat ttcagtgatg cctttttctt tgacaatgtt ccagtgttta gcacgcaagt
    34321 cttttgctgc tgctaaaagt tcttcttctg agatttcttt tctaaagtat ttttcagttg
    34381 taaattttaa ttcacggaat tcgcccaaac gagggaaacc gatgattgta gttgacatga
    34441 tgtgtcctcc aaaatttgtt gttgaaacta tcttaacaga aaagaaagcg tctgtataat
    34501 tgtaaaaaat tagggtttga tatagtttga aactatatat ctgtttcgga caaaagaaaa
    34561 agacttgaag caaacatctc aaatcctttg taattcttac tttacagcta tattccaatt
    34621 agaatactaa aacatgttat tagtaattct tataagtgac tatgacctgt tattagaaaa
    34681 gactataact gattctagtc aacttttccc ctgttcaagt ggaacgactg ctagtgtctt
    34741 tcctagactg gttagaactt ttaagaccgt atccaactga agactgctct ctcctgtctc
    34801 catcctagct atgacaggct ggcttattcc actgacttct tccagctttt tctgactgat
    34861 tccttgttca tacctagcct caatcaactc gctcatgata gccactcgca tatcactttc
    34921 aaggatttcc tccttgctaa agagctcaga tggacatcct tccaattact cccaatagca
    34981 ctattcttca tcacttaacc ctcttttttt tacgtctatg tatttttaaa aaaatgagcg
    35041 aattatgatt cgatagattg accagcgggt ttaaagttgg tgctagccta tttcttaagc
    35101 gattttcctt ttctaggata aagcagatcc tgcttgctta accccaattt tccacgatga
    35161 attcaatagt aaatggttga aattcccacg ttaacccctt tagccatcac catcatttca
    35221 ggcgaaaatt tttggttatg tatagtggag aatcttttcc tttagtttct taagactgtt
    35281 gagcgtagtc ggcagaataa atctctttga agcgcccttt tccaagacat tgtcggactg
    35341 tcccacgctt gatttcagtg tggatagttt gaggagcttt tccaagtaga gaggcaattt
    35401 ctctatttga ttttccttct tttttccatc gttcgattaa gcgacggcta tcgattgtca
    35461 aatgtttgcc ttttgtagta taattgtctt gcatttctgt gccttttaat catttcaatc
    35521 ttaaattgaa cttttttact tgggttgtac ttaatctatg aggaagacaa gaaaaagaat
    35581 atcaatcaag taaagtcaca ttagctccga gcaaccattg caaattgagg tactcacaca
    35641 atgattaaaa catttctctc tgccctttcg gtcattctct ttgttatccc tatcatagct
    35701 tattcttttt ttccatcttc taatcttaat atttggctat ctaccagacc tatcttggca
    35761 cagatttatg ccttcccctt agctactgca actatggctt ctattttaag tttcgtattt
    35821 ttttccctat ctttttacaa gaaaaataaa caaatacggt tttactctgg cattttgctc
    35881 ttactatcgc tcatattact attattcgga acagataaaa ccctttcttc tgcatcaaat
    35941 aagactaaaa ccttaaaatt agtaacttgg aacgtcgcta atcaaataga agcacaacat
    36001 attgagcgaa tttttagcca ttttgatgcc gatatggcta tattccctga actagctacc
    36061 aatatcagag gtgagcaaga aaaccagaga atcaaactat tgtttcatca agttggactt
    36121 tctatggcca actatgatat tttcacttct ccacctacca atagtggaat agctcctgtg
    36181 actgtgattg tcaagaaaag ttatggtttc tatacagaag ctaaaacttt tcatacaaca
    36241 cggttcggga caattgtatt acattcgaga aaacaaaata taccagatat cattgccttg
    36301 catactgctc ctcctctgcc aggtttaatg gaaatctgga agcaagactt aaacatcatt
    36361 cataatcaat tggcttcaaa atatccaaag gctattattg caggtgattt taatgcaact
    36421 atgcgtcatg gagcacttgc aaaaataagc tctcataggg acgcattgaa tgcacttcca
    36481 ccttttgaaa gaggaacttg gaatagccaa agtccaaaac tttttaatgc aacgatagat
    36541 catatcttat tgcctaaaaa ccactactat gttagagatt tagacattgt aagttttcaa
    36601 aactctgatc atagatgtat ttttacagaa atcacatttt aattatttta tataaaatca
    36661 cccctctagt gttcataaac tagaggggga atttgtatca tactatcgtt taacgcactt
    36721 ctgtattcac tttttcttcg agcgatactt ggattttttc catatagcgt gcgacttctt
    36781 cgtccgttaa gctatcttct ggattttgga aggtcaagct ataagccatt gacttcatac
    36841 caagtcccaa tttctcacct gagaagacgt caaagagttt gatatctgtc aaacgtttca
    36901 cgccggcagc ttggatagca tctacaactt cttgatgagt cacttctgcc ttgaggagaa
    36961 gggcaacgtc acggctgact gccgggaatt tggtgatttc tacaaatgga gtcgctggct
    37021 gaagcgcagc ttcgatagct gaaaggttaa gctcagctac atacgtttct ggaatatcat
    37081 aagccttagc agtgactgga tgcacttggc caaggaaacc aagaacttgg tcaccgagtg
    37141 aaatgactgc tgtacgacct ggatgaaggc tagcgatttc agatgttgct gtataggtta
    37201 cttggagtcc caagcgagta aatagggctt caaggattcc cttagcatag aagaaatcaa
    37261 ctggaactgc tgctgtttgg aaatcttttt cagcaaccaa gcctgtcaag gcaaaggcaa
    37321 aactgttaat ttcatttgga agttcttctc ttggattacc tgtttgttca aagacttttc
    37381 caatctcgta aagggccaag tttttattct tacgagccac gttgtaggca acggtatcaa
    37441 ggatacctga aatcatattt tgacggagga ctgaacgatc cacagtcatt ggccacatga
    37501 gttccgtaag gttacttggt tgagccgtaa actcaactgc tttttcagga gttgttagag
    37561 cataggtgat gatttctgtc aaacctgctc cttcagcaat ggtacgaact tgacggcgga
    37621 gtttttgtgt cacagtcaat tcaccagctg taccatcgtc ttttggtaga ctagttggca
    37681 agcggtcata tccatagata cgagcgattt cttcaaagag gtcagcttcg attgtgatat
    37741 cccaacgacg acgtggtacg ctgactgtaa agctgtctgc atttccagaa agaccaaaac
    37801 caagacgacg gaagacgtct tcgacatcag cataagacag ctcagttccg aggacacggt
    37861 taacatcagc aagggttgaa gaaacttcca catcagaggt atcaagctca cccgctgaaa
    37921 cgataccctt acgcaccgtc gcacctgcaa gttctgcaat catgctagct gccgcatcaa
    37981 gggcttcatt gactgttgcc acattgatgc ctttttcaaa gcgagaagac gattctgaac
    38041 gaaggttcag gcgaccactt gtcttacgga tagatttacc attgaaaaca gcagcttcaa
    38101 ggacaacacg actagatttt tcagagattt ctgttgcttg accacccatg acacctgcaa
    38161 gggctactgg cttgtctgca acagtgatga ctaggtcatt cacgtccaag tcacgttctt
    38221 caccgtccaa ggtcaccaat ttttcaccag cacgcgcttc acgcacacgg atgtcagtcc
    38281 cttcaaatgt gtccaagtca aaagcatgca taggttgacc aaagtagagc aggatgtagt
    38341 ttgtcacgtc tacaacgtta ttgatgggac ggatgccttc gttcatgaga aggttttgca
    38401 accattgtgg acttggtgcg ataatcacat tgtccaagat acgagctgca tagtaaggcg
    38461 ccttgtctgt cccaatgctg acagaaaggg catctgccgc agcttcatta gtttctgtta
    38521 gagtaaattc tttaaagttg actgccttgt catagatggc tgccacttcg tgagccactc
    38581 cacacataga aagggcatct gcacggtttg gtgtgatgga aagttcgatg atttcatcat
    38641 ccaagtctag gtaagaaaag acttcctcac ctggcacggt attttctggc aagatttgga
    38701 tgccatctgc gaattcctta ggcacaactg agtcagaaat tcccaattca ccaagtgaac
    38761 agatcattcc aagtgactcc aaaccacgga tttttccttt tttgattttg tagttatcag
    38821 cgatacgagc tcctggaaga gccaccatga ccttgatccc agcacgcaca tttggggcac
    38881 cacaaacgat ctgacgggct tcttcttcgc caacgttaac ctgacaaaca tggaggtgag
    38941 tctctggcac atcttcgcaa gacaagacct caccgacaac aatttttgag agatcagcag
    39001 ctggtgattc gacactctct acctcgatcc ctgtggttga cattttttca gccaactcct
    39061 gtgatggcac atcaatgtcc accaattctt ttaaccattt ataagataca agcataattt
    39121 agttctccag aatgaccatt gccacactgg ttcttttcct ttcctatcat ttcaatagaa
    39181 gaatcctctt cttaccttaa tttctttctc agtaaccaat ccgtatctac tttttgacca
    39241 accataaaat gatgttggct aaatttttca aaaccatatc gattataaaa cgcttgagct
    39301 tttgtattat gctcccaaac acctagccaa gcccaagaaa aactattttt tgtagcaagt
    39361 tcaagtgcga attcaaacag ttgcttacct attccaaatc cttggaattt ttgtagcaca
    39421 tagaggcgtt gaatttcaaa agcgtcctct aattctctct cagtttgagc acttccccag
    39481 ttgactttga gaaaaccagc tatctcctcc tcatgcataa tgaaataggt ttcagagtca
    39541 ggatttccca actcagttga caaagttttc agactataag cctcttcaaa gtattcctgt
    39601 aactgctctt ccgtattatc atacgcaaag gtttcacgaa aggcttgttt ggcaatttta
    39661 gccaacacct caacatctgc catttctact tttctaatca ttatttaaac tgttctgaga
    39721 agcggacatc tccttggtag aatccacgga tatcgttgat tccataacgg agcatagcta
    39781 cacgctcttg tccaagacca aaggcaaaac cagagtatac agtcgcatcg ataccactca
    39841 tttcaaggac acgtgggtga accataccgg ctcccataat ttcgatccaa cctgttttct
    39901 tacatacgtt acagccttct ccaccacact tgaagcaaga aacatccacc tcaacagatg
    39961 gctctgtgaa tgggaagtaa gatggacgca aacgaatttg acgctcttca ccaaacattt
    40021 tttggacaat caactgaagc gttccttgaa gatcagccat agagatattt ttcccaacta
    40081 ccaagccttc gatttggtgg aattggtgac tgtgggtcgc atcgtccgta tcgcgacgga
    40141 agacacgccc tggcgagatc atcttcaaag gacctttaga aaaatcatgg gcatccatag
    40201 cacgcgcctg aactggagac gtgtgggtac ggagcaagat ttcttcagtg atatagaaag
    40261 tatcctgcat atcacgagct gggtggtctt ttggaaggtt catacgttca aagttatagt
    40321 agtcttgctc tacttcaaaa ccatccacga cttgataacc cataccgatg aagatatctt
    40381 cgatttcttc actggtttgt gtcaaaacgt gacggtgacc agtcgcaact ggacgacctg
    40441 gaagcgtcac atcgatactc tcgctagcca gttgagccgc gactttcttt tcttccaaga
    40501 gcttagctgt ttcttcaaaa gcagcagtca agacatcacg agcttcattg acgtgtttcc
    40561 cgatgattgg acgcatctca gcagaaacat ctttcatccc tttgaggatt tcagtgagcg
    40621 aacccttttt accaaggaca gagacacgca aatcttgcat ctctttttca tttccagcag
    40681 taatctgctt caagctagcc agcgtttctt cgcgaagcgc ttttaattgt tcttcaatag
    40741 ttgacatatt tcctccatca gtctctcgta gataaaaaga aaaccacatg ccaaaaactc
    40801 cactcggagc gttgacacgc ggtaccatcc gttttcatct gacaagtcag accttcattt
    40861 ctaaatccat gcgcaagtga attcacccag ctttcatata gagagcttgc agtcacggct
    40921 ctcctccctg atatacttcc cttgggctac tagtctttca gattcctatt caattactac
    40981 ttagtttatc agatttttac cattcttgca agacctatct tacttctgct tgttagctta
    41041 ttcttatcta actttatagc ggattgaaat aagatgaaaa caaatc
//
LOCUS       DAVAAL010000004        39984 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVAAL010000004 DAVAAL010000000
VERSION     DAVAAL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.004"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(181..594)
                     /gene="ndk"
                     /locus_tag="VQM60_000131"
     CDS             complement(181..594)
                     /gene="ndk"
                     /locus_tag="VQM60_000131"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET1080045.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(705..4382)
                     /gene="rpoC"
                     /locus_tag="VQM60_000132"
     CDS             complement(705..4382)
                     /gene="rpoC"
                     /locus_tag="VQM60_000132"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET1080046.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(4415..8026)
                     /gene="rpoB"
                     /locus_tag="VQM60_000133"
     CDS             complement(4415..8026)
                     /gene="rpoB"
                     /locus_tag="VQM60_000133"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET1080047.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            8442..8512
                     /locus_tag="VQM60_000134"
     tRNA            8442..8512
                     /locus_tag="VQM60_000134"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8474..8476,aa:Cys,seq:gca)
     gene            complement(8710..10041)
                     /locus_tag="VQM60_000135"
     CDS             complement(8710..10041)
                     /locus_tag="VQM60_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET1080048.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(10332..11156)
                     /gene="endA"
                     /locus_tag="VQM60_000136"
     CDS             complement(10332..11156)
                     /gene="endA"
                     /locus_tag="VQM60_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET1080049.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSNATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(11195..11383)
                     /locus_tag="VQM60_000137"
     CDS             complement(11195..11383)
                     /locus_tag="VQM60_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET1080050.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(11376..12659)
                     /gene="murA"
                     /locus_tag="VQM60_000138"
     CDS             complement(11376..12659)
                     /gene="murA"
                     /locus_tag="VQM60_000138"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET1080051.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(12683..12913)
                     /locus_tag="VQM60_000139"
     CDS             complement(12683..12913)
                     /locus_tag="VQM60_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET1080052.1"
                     /translation="MIQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(12971..14008)
                     /locus_tag="VQM60_000140"
     CDS             complement(12971..14008)
                     /locus_tag="VQM60_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET1080053.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFTPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(13992..14480)
                     /gene="coaD"
                     /locus_tag="VQM60_000141"
     CDS             complement(13992..14480)
                     /gene="coaD"
                     /locus_tag="VQM60_000141"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET1080054.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDMIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKAVKHLGNVKVVSSHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(14470..15009)
                     /gene="rsmD"
                     /locus_tag="VQM60_000142"
     CDS             complement(14470..15009)
                     /gene="rsmD"
                     /locus_tag="VQM60_000142"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET1080055.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(15074..16066)
                     /locus_tag="VQM60_000143"
     CDS             complement(15074..16066)
                     /locus_tag="VQM60_000143"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET1080056.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(16178..16861)
                     /locus_tag="VQM60_000144"
     CDS             complement(16178..16861)
                     /locus_tag="VQM60_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET1080057.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(16917..17657)
                     /locus_tag="VQM60_000145"
     CDS             complement(16917..17657)
                     /locus_tag="VQM60_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET1080058.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            17691..17969
                     /locus_tag="VQM60_000146"
     CDS             17691..17969
                     /locus_tag="VQM60_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET1080059.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            18047..18973
                     /gene="yidC"
                     /locus_tag="VQM60_000147"
     CDS             18047..18973
                     /gene="yidC"
                     /locus_tag="VQM60_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET1080060.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(19048..19842)
                     /gene="pflA"
                     /locus_tag="VQM60_000148"
     CDS             complement(19048..19842)
                     /gene="pflA"
                     /locus_tag="VQM60_000148"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET1080061.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(19968..21218)
                     /locus_tag="VQM60_000149"
     CDS             complement(19968..21218)
                     /locus_tag="VQM60_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET1080062.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPEEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(21285..22112)
                     /gene="purR"
                     /locus_tag="VQM60_000150"
     CDS             complement(21285..22112)
                     /gene="purR"
                     /locus_tag="VQM60_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET1080063.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(22379..23320)
                     /locus_tag="VQM60_000151"
     CDS             complement(22379..23320)
                     /locus_tag="VQM60_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET1080064.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(23310..24566)
                     /locus_tag="VQM60_000152"
     CDS             complement(23310..24566)
                     /locus_tag="VQM60_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET1080065.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDNLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(24568..25230)
                     /locus_tag="VQM60_000153"
     CDS             complement(24568..25230)
                     /locus_tag="VQM60_000153"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET1080066.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTAEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(25193..25849)
                     /locus_tag="VQM60_000154"
     CDS             complement(25193..25849)
                     /locus_tag="VQM60_000154"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET1080067.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRQEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(25860..26738)
                     /gene="rsgA"
                     /locus_tag="VQM60_000155"
     CDS             complement(25860..26738)
                     /gene="rsgA"
                     /locus_tag="VQM60_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET1080068.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(26740..27612)
                     /gene="rsmA"
                     /locus_tag="VQM60_000156"
     CDS             complement(26740..27612)
                     /gene="rsmA"
                     /locus_tag="VQM60_000156"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET1080069.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(27998..28156)
                     /locus_tag="VQM60_000157"
     CDS             complement(27998..28156)
                     /locus_tag="VQM60_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080070.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEG"
     gene            complement(28149..28790)
                     /locus_tag="VQM60_000158"
     CDS             complement(28149..28790)
                     /locus_tag="VQM60_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080071.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(28792..30822)
                     /locus_tag="VQM60_000159"
     CDS             complement(28792..30822)
                     /locus_tag="VQM60_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET1080072.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEVLSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQFICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGLSATALLPNYREMQEMEKASDKWSQSSDRYRLSFGW
                     SSAFADEEGMRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGVEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(31090..31989)
                     /locus_tag="VQM60_000160"
     CDS             complement(31090..31989)
                     /locus_tag="VQM60_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1080073.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEELMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(32061..32621)
                     /gene="rnmV"
                     /locus_tag="VQM60_000161"
     CDS             complement(32061..32621)
                     /gene="rnmV"
                     /locus_tag="VQM60_000161"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET1080074.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(32621..33394)
                     /locus_tag="VQM60_000162"
     CDS             complement(32621..33394)
                     /locus_tag="VQM60_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET1080075.1"
                     /translation="MIFDTHTHLNVEEFAGRETEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(33699..34592)
                     /locus_tag="VQM60_000163"
     CDS             complement(33699..34592)
                     /locus_tag="VQM60_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET1080076.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLETTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKNKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(34876..35010)
                     /gene="rpmH"
                     /locus_tag="VQM60_000164"
     CDS             complement(34876..35010)
                     /gene="rpmH"
                     /locus_tag="VQM60_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET1080077.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(35155..36369)
                     /locus_tag="VQM60_000165"
     CDS             complement(35155..36369)
                     /locus_tag="VQM60_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET1080078.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSNSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLASDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(36525..36817)
                     /locus_tag="VQM60_000166"
                     /pseudo
     CDS             complement(36525..36817)
                     /locus_tag="VQM60_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36979..37431
                     /locus_tag="VQM60_000167"
     CDS             36979..37431
                     /locus_tag="VQM60_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET1080079.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(37466..38854)
                     /locus_tag="VQM60_000168"
     CDS             complement(37466..38854)
                     /locus_tag="VQM60_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET1080080.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQTLDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            38920..39882
                     /locus_tag="VQM60_000169"
     CDS             38920..39882
                     /locus_tag="VQM60_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET1080081.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVNTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
BASE COUNT        11239 a         9381 c         7722 g        11642 t
ORIGIN      
        1 gcatagcggg tgtttttttg tctcgcacct aacggagcga gacaaactaa tagtcactta
       61 atcaaaaaac gcaccatatc aaaaactaaa aagtttgata tcatgcgtca tgtcttaaac
      121 taattgacta tactttctat tcaaatgagc ttttaaccaa ttgattgagc caatccactc
      181 ttaaaaccaa agagcaattt ctcgcttagc tgactcttct gaatctgaac catgtacaac
      241 attttggata atctcatttt ctccagcagc ttttgcaaaa tcacctcgaa tagtgcctgg
      301 taaagcttct tctggacgag ttgcacccat catggtccgc caagtttcga ttactttggg
      361 accagaaatg acacccacaa gaactggacc tgaagtcatg aattcacgaa tcggtgggta
      421 aaaactctga ccaaccaagt cctgatagtg ctggtcaatc aactcttctg aaacctgtga
      481 acgaaactcc aatttttcga ttgtaaatcc acgttgttcg atgcgcttta acacttcacc
      541 cactagccct ctttttacac catctggttt gatgataaag aatgtttgtt ccatacccgt
      601 ctcctttgtc agcttctttc ttttatttta ccacatttcg tggaaaaatg gagaaagttt
      661 tcagaagaga gaatgagaga accctcgggt tctctcattc tctcttattc tactgtttct
      721 tccacagttt caacggcagt atccacaact acttctgttg tttcttcatt tccttcttcc
      781 tctactggag gattaaggta ttcttcttcg ttgacagcat gtggttcaag gttacggtaa
      841 cgggccatac cagtaccagc tgggatgatc ttaccgatga taacattttc tttaagtcca
      901 aggagatggt ctttcttacc acggatagct gcgtcagtaa ggacacgagt tgtttcctgg
      961 aaggaagccg ctgacaagaa actgtttgtt tcaagtgagg ctttggtaat tcccataagg
     1021 actgggcgac ctgtcgctgg aactccacct gcgataagga catctttgtt ggcatctgta
     1081 aagtcattga tatccatgag ggtacccatg agaagatctg tatcacctgg atccatgaca
     1141 cggactttac ggatcatttg acgaaccatt acctcgatgt gtttgtcacc gatttctacc
     1201 ccttggctac ggtaaacttt ttgtacttca ccgagaaggt acgtttcaac tgacaagaca
     1261 tcacgaactg caaggagacg ttttggttgg atagaacctt ctgtcagagc agcaccacgc
     1321 gctacttggc ccccaacttc gacacgcata cgagctgtaa atggaacgac atattcacct
     1381 tcgccagttt cacccttaac aaagactttc ttggtacgag ttgatgcatc ttcttcgata
     1441 gcagtaactt gtcctttaac ctctgttata accgcttccc ctttaggatt gcgggcttca
     1501 aagatttctt ggacacgagg aagaccctga gtgatatcgg tatttgaggc aaccccacct
     1561 gtgtggaagg tacgcattgt aagctgtgta ccaggttccc cgatagattg ggcagcgatt
     1621 gtaccaactg cttcaccaac ttcaaccgca tcaccagtcg ccaagttgat accataacag
     1681 tgacggcaga caccgtgacg agtgttacat gtaaatacag aacggatagt cacttcttcc
     1741 acaccagcat tgacaatttc acgcgccttg tcttctgtaa tcaattcatt tggaccaata
     1801 atcactgcac cagtttctgg atgtttaaca gttttcttag tgtaacgacc gttgagacgc
     1861 tcttcgagag actcgatcat ctcttttcct tctgcgatag aacggatcaa gagaccacgg
     1921 tcagttccac agtcgtcctc acggatgata acgtcttggg caacgtcgac caaacgacga
     1981 gtcaagtaac ctgagtcggc tgtcttaagg gccgtatcgg tcataccttt acgagcaccg
     2041 tgagttgaga agaacatttc caataccgac aaaccttcgc ggaagtttga aagaattggc
     2101 aattccatga tacgtccatt cggagcagcc atcagaccac gcataccggc aagctgtgag
     2161 aagtttgaga tgttaccacg ggctccagag tccatcatca taacgattgg gttcttagga
     2221 tcttggttag caatcaagcg tttctcaagt ttttcacggg cagcacgcca ttcagctgta
     2281 acagcattgt aacgctcgtc gtctgtgatc ataccacgac ggaattgttt ggtgatttgt
     2341 tcgacacgtt tgtgtgattc ttcaatgatt tcagccttgt catcaacgac tgggatgtcg
     2401 gcaataccca ctgtcaatcc tgcaagagtt gagtggtggt aaccgagatt cttcatgcgg
     2461 tcaagtaggg cagaagtttc tgtcgtacgg aaacgtttga agatttcagc gatgatattt
     2521 ccaaggtttt ttttcttgaa tggagggttg agctcaagat tgctgatagc ttccttgata
     2581 tctccaccaa gtggcaagaa gtatttagct ggaacacctt ctgtcaagtt ggcattgttt
     2641 ggttcttgca agtatggtag cccctctggc atgatatcgt tgaagagaat tttaccaact
     2701 gttgtaagca agaccttatg tctttgctct tctgtccaag gcttgttgag gctgtctgtt
     2761 gcgataccaa cacgtgagtg gaggtgaaca tagccattgc ggtaagccat aaccgcttcg
     2821 tcacggtctt tgaagaccat tccttcacct tcgcgaccag cttcttccat ggtcaagtag
     2881 tagttaccca aaaccatgtc ctgagatgga gtaactaccg gtttcccatc tttcgggttc
     2941 aagatgtgct cagcagctag catgaggata cgagcttctg cttgtgcttc ttctgaaagt
     3001 ggtacgtgga tggccatttg gtccccgtca aagtcagcat tgtaggcttc acagacaagt
     3061 gggtgcaagc gaagagcctt accatcaatc aagactggct cgaaggcttg gatacccaaa
     3121 cggtgaaggg tcggtgcgcg gttcaaaagc actgggtgtt ctttaatcac ttcttcaagg
     3181 atatcccaga tacgctcatc tccgcgttcc accaagcgtt tagctgcttt gacgttttgc
     3241 acgatatcac gggcaacgat ttcacgcatg acaaatggtt taaagagttc aatcgccatt
     3301 tcacgtggca caccacattg gtacatctta agagttggac caacagcgat aacggaacgt
     3361 cctgaaaagt caacacgttt accgagcaag ttttgacgga agcgtccttg tttaccttta
     3421 agcatgtggc tcaatgattt caatggacgg ctacctggtc ctgtgattgg acgaccacgg
     3481 cgaccattgt caatcaaagc gtcaactgct tcttgaagca tacgcttctc attttgaacg
     3541 atgatacctg gtgcatttaa ctcaagcaaa cgagccaaac ggttgttacg gttgataaca
     3601 cggcggtaaa ggtcattcaa gtcagatgag gcaaaacggc caccatccaa ctgcaacatt
     3661 ggacgaagat ctggtggaat aaccggaagg atgttaagaa tcatccattc aggtttgttt
     3721 ccagacttgt aaaaggcatc caaaacatcc aaacgacgga tggctttgac acgtttttgt
     3781 ccagtagctg ttttcaattc ttctttgagt tcagcaattt ctttttcaag atctacttgc
     3841 ttcaaaaggt cttggatggc ttccgcaccc atcttggcaa caaatgaacc ataaccatat
     3901 tcacgcaagc gctctcggta ttcgcgctct gtcatgatag acttgtgctc aagtggtgta
     3961 tccttaggat caatcaccac ataagccgca aagtagataa cttcctcgag ggcacgaggg
     4021 ctcatatcaa gggtcaagcc catacggctt ggaatcccct tgaagtacca gatgtgagat
     4081 acaggagctt tcaattcgat atgtcccata cgctcacgac gaactttcgt acgcgttact
     4141 tcaaccccac agcggtcaca aacaattcct ctgtaacgaa tgcgtttgta cttaccacaa
     4201 gcacattccc agtcttttgt aggaccaaag atcacttcat caaagagtcc ttcacgttct
     4261 ggtttcaagg tacggtaatt gattgtttca ggttttttga cttctccata agaccatgaa
     4321 cggactttac ttggagaagc tagggtgatt tgcatacttt taaaacgatt tacatcaacc
     4381 actatttctt ccctttctat tctaagtgaa ctgcttattc ttgttcagca gcttcttctg
     4441 ttgcttccgc ttttgttgct ttctcagctt cttcagcttc aaaggctgct ttagcctctt
     4501 gggctgcttt ttcgcgggct ttttcaaggt catctacgtg gatgacatct tcgtccattc
     4561 cttcatccaa gtcgcgaagt tccacttctt ggtcatcttc gtctaggaca cgcatgtcaa
     4621 gaccaagaga ttgcaattct ttgacaagaa ctcggaagga ttctggaaca cctggttttg
     4681 gaattggttt gccttttgta atagcttcat aggctttcaa acgtccgttg atatcgtcag
     4741 acttgtaagt caagatttct tgaaggacat ttgacgcacc gtaggcttca agagcccaaa
     4801 cctccatctc accgaaacgt tgtccaccaa actgagcttt acctccgagt ggttgttggg
     4861 taacagttga gtaaggtccg actgaacgcg cgtgcaattt atcgtcaacc atgtggtgga
     4921 gtttgatcat gtacatgact ccaacagaaa cacggttatc aaatggttca ccagtacgtc
     4981 catcgtaaag gatcgttttg gcatcgctat ccatacctgc ttctttaaca gttgaccaaa
     5041 gatcttcaga acttgctcca tcaaagactg gtgtcgcaat gtgaatacca agagtacgag
     5101 ctgccatacc aaggtgaagc tccataacct gaccgatatt catacgtgat ggcaccccaa
     5161 gtgggttcaa catgatgtcg actggagttc cgtctggaag gtaaggcatg tcttctacag
     5221 gaacgatacg agagacaacc cctttgtttc cgtgacgtcc ggccatttta tctccgacct
     5281 taatcttacg tttttgagcg atgtaaacac gaaccaacat gttaacacct gattgcaact
     5341 catctccatt tacacgtgta aagatcttaa catcacgaac gacaccatcg gcaccgtgtg
     5401 gtacacgaag agaagtatca cgcacttcac gagacttgtc tccaaagata gcgtgcaaga
     5461 gacgttcttc agctgaaaga tctttctcac ccttaggtgt tactttacct acaagaatat
     5521 caccttcttt aacctcagca ccaatacgga taatccccat ttcgtcaagg tctttgaggg
     5581 catcttcacc aacgtttgga atttcgcgag tgatttcttc aggcccaagc tttgtatcgc
     5641 gcgtttctga ttcgtattct tcaaggtgaa cagatgtgta gacatcgtcc ttcaccaagc
     5701 gttcgctcat gataacggca tcctcgaagt tgtaaccttc ccaagtcatg taggcaacga
     5761 ttgggttttg tccaagcgcc atttctccat tttccataga aggtccgtca gcgatgaaat
     5821 cgcctttttc aacgacatca ccaactttta cgagagtgcg ttggttgtaa gcagtacctg
     5881 agtttgaacg acggaatttt tggatgtggt aaacatccaa tgatccatct tcacgacgaa
     5941 cttctacctt gtcagcatct gcgtaagtaa ctttaccatc atactgagca atcacagccg
     6001 caccagaatc gtgggctgct tggtattcca taccagtacc aacgtaaggt gcctgaggat
     6061 taatcaatgg cacagcctga cgttgcatat tggctcccat gagggcacgg ttggagtcat
     6121 cgttttccaa gaaaggaata catgctgtcg caacggcaac tacctgtttt ggtgaaacgt
     6181 ccatgtagtc aacaatatta gctggatact cttggttgac cccttggtga cgtcccatga
     6241 caatcttctc agcaaaggtt ccatcttcat tcagacgaga gttagcctga gctacagtat
     6301 attcatcttc ttcatcagct gtcaaccaaa caatttcgtt cgtgacaaca cctgtttcac
     6361 ggtcaacctt acggtatggt gtttgaacaa aaccatattt gttcaagtgt ccgtaagatg
     6421 acaagttatt gatcaaaccg atgttaggtc cttcaggtgt ctcgattgga cacatacgac
     6481 catagtgagt gtagtgcacg tcacgtactt catatccagc acggtcacga gtcaaaccac
     6541 caggtcctaa ggctgacaaa cggcgtttgt gagacaactc agaaagcggg ttgtgttggt
     6601 ccatgaactg tgacaactgt gatgaaccaa agaattcttt aactgcagct gttacaggac
     6661 ggatattgat aatttgttgt ggtgtcaaga cttcattgtc ctgaacagac atacgttcac
     6721 ggacattacg ttccatacga gaaagtccca aacgtacttg gttggcaagc aattcaccaa
     6781 ccgcacggat acgacgattt ccaaggtggt cgatatcatc tacacggcca agtccttcag
     6841 ccaagttgag gaagtagctc atctcagcaa ggatatctgc aggagtcacc gtacgaacct
     6901 tgtcatctgg gttagcatta ccaatgatcg ttacgacgcg atctggatca gttggagcaa
     6961 caaccttgaa tttttgaaga acaacaggct cagtcacaac ggctgcatcg tttgggatgt
     7021 agacaatctt gttcaagtcg ccatccaaat ggctttcaat gctttcaatc acgctacgag
     7081 tcataatcgt accagcttct accaagattt ctccagtttc agggtctacc aatggctctg
     7141 caatggtttg gttgagcaaa cgtgttttaa cattgagttt tttattgatt ttgtaacgac
     7201 caactgctgc caagtcatag cgacgtgggt caaagaagcg agccacaagc aagctacgtg
     7261 agctttcagc tgtcttaggc tcacctggac gaaggcgttc gtaaatttct ttcaaggctt
     7321 cgtctgtacg agagtccatt ggatttttgt ggatatcttt ttcaacagtg ttgcgaacca
     7381 attcgctgtc accaaagata tcaaagattt catcatcacc tgagaaacca agagcacgaa
     7441 ccaaggttgt aaatggaatc ttacgagtac ggtcgatacg agtgtaggtg atatcttttg
     7501 agtcgctttc aagttccaac caagctccac ggttagggat aacagttgaa ccatagccca
     7561 ccttaccatt tttgtctact ttgtcgttaa agtaaacacc tggtgagcgg accaactgag
     7621 aaacgataat acgttcacca ccattgatga tgaaagtacc catttctgtc atgattggga
     7681 aatcaccaaa gaaaacttct tgggtcttga tttcgcctgt ttctttattg atcaaacgga
     7741 aggttacaaa aattggtgct gagtagctag catcgtggat acgagcttct tctagcgtgt
     7801 attttggttc cttgatttca tatccaacaa attccaactc cattgtgtct gtgaagtttg
     7861 aaattggcaa tacatcttca aacacttcct taagaccgtg gtctaggaaa gctttgaatg
     7921 agtcagtttg aatttcaatc aaatttggta agtcaagaac ttctttgatt cttgaaaaac
     7981 tacgacgggt acgatgtttc ccgtattgaa cgtcatgtcc tgccaagatg attctccttt
     8041 gtaaataagt tccaagcctt gtcaatcagg cttttctaat cgtcatatgg ttgtaaaccc
     8101 cttatcaccg tgtccttttg acgaattttc agaatcttta agcctctgtt acaaatgctc
     8161 aaaatcttga aaaaaagcac aaaaagagca gctaaatctg actttttcag aagatttaac
     8221 tgctgtgagc cttgtctgga caatatttca gacaaaacct acgacaaatg attacccata
     8281 ttatacccta tttagctaga tttttcaagg ggtttcagta ggtttttggt aaattttttc
     8341 ccatagaaaa cttggcatca cattcgaatc acgctatggt acaaaaaact gaaaaaacta
     8401 ttgactgaaa atcattttca aggtataata ataaacgtta aggcggtata gccaagtggt
     8461 aaggcacggc tctgcaaaag cttgatcgtc ggttcaaatc cgtctaccgc cttctataac
     8521 ttgatttatc aggtttcaaa tgaacagaaa gcccaatttg aagggctttt cttattttcc
     8581 ctcgaataaa tacgtataac tttaaaaact tttggagcga gtttgtggca gagttctttc
     8641 catggcataa ttcccttttg aaatcaattg aaaaaagctt cgtcacgcct ggtgacaaaa
     8701 gcctctagtt tattctgttt cttcttctat ttcgacttct gtgatttgga cttttacctt
     8761 ggtaacacgt ccattcttca ctttatcatt ggttaagata aactgtttgt tttggctgac
     8821 caattcatag ctgagtttct cagtcgttgg aatagttccc actcctgtca aataataacc
     8881 agcgatggta tcaacgtcat cactttccag ttcaacatca aagtaattat tgaaatcatt
     8941 gagggtcata gttccttgaa caatgtaggt atcctcgcca atctgatgaa catctattgc
     9001 tgctttgtcc gtttcatcat caatttcacc aacaatttcc tctaagaggt cttccaaagt
     9061 caccaaacca gccataccac cgtattcatc cagcaagatt gccatttgtc tttgggtatt
     9121 tcgcagttct tttagcaagt catccacaaa aatagtttca ggtacaaaaa gtggatcttg
     9181 taaaattctc ttccaaacaa tattgtcaaa accgtccaca aagcctgcct taaggagact
     9241 cttggtgtga atgattccaa ttacattgtc cttatcccca tcataaaccg ggatacgaga
     9301 ataattttgt tttaaaatac tttggataat ggcttgacta tcatcctgaa tatccaccat
     9361 aaaggcatcc gttcgaggca ccataacctc tcgtgccatc agttcatcga gcgaaaagac
     9421 accttgtagc atctcaatct catcagcatc caatgtttct tcactatttg tcagcatata
     9481 ggcaatttca tcacgggtca tcttttcatc cgcatcatcg aatgacatag gagtcaaatg
     9541 gctcaagaaa ttggtcgaag cagctaaaag ccaaacaaaa ggactgacta gttttccgat
     9601 cccaatgata atcggcgctg tacgaattgc caaggcatcc tttagattaa gagcgattct
     9661 cttaggatat aattccccaa aaacgatgga aatataggtc aaaaatgcca aggatagaaa
     9721 agttgccacg gcttgtgctg tttcgccatt cccaagccaa gaggcaatca cacgtcctag
     9781 agtatcagtt aaactcgccc ctgataagat tgtaatcagg gtgattccta cctgaatggt
     9841 tgataaaaag tggttaggat tttctagtac cttcagcagg cggatgtagc gtctgtctcc
     9901 ttcttccgcc ttttgttcaa ctcgggcacg attaagagaa accatggcca tttctgtggc
     9961 tgagaaaaag gcatttaata ccgtcaagat aaacaataaa acaaattgta gcaacaaatt
    10021 ctgactactt gggtcttcca tagttcttct cctaaaatag tatcttatcc tttattatat
    10081 cacaaaattt aatccaatac atattatttt tttcttagcg taacttccat ctctctccca
    10141 aaactggaag ttagtctaag acggatttcc actgatttca cgcgtttttc tcatactaaa
    10201 agctaggttg tgggaaactg gaagttagtt ttagaagtta ctattttttc ttagtaccta
    10261 ttctagccct gcttaaacta gtaaaaagaa ctcctacatc cataagtgac ataggagttt
    10321 aggcttatct tttactgagt tacagttact tctccagttc ggtaatccag ttgaagtccc
    10381 ttttgaacat tgggaactag aacattgaat tccaattctc catccgaaga cttggcttca
    10441 atctgtgaag ctgagggaac taaatcctcg tttgaagcgt agtaaagggt tacacggtaa
    10501 cggacacgct tgttttggtc caaggcttta cgcaccttgc tttcatagta gttttgacca
    10561 gtcgaatact cggcttgtgc ctgatttgcc caggctgtct gaacagcaat gtttttagga
    10621 ttgcttgttg aggcatcaaa accatccaaa ccaccgatta aggcatagcc taacaaatga
    10681 cctctatcga ctgcatgggt ataagagccc tttagattct tgacctgatg ccaacctgga
    10741 ggagtccaag aagttgaacc attcccagtt tctttacgat tcttgtactg acgagtggca
    10801 ttagacaaga gggcattagc tacggttgga acagtttcct tgcccactgt ctttgtttta
    10861 ttgtcagcgt agggcttact tgaaaccttg gcatctagat ttgttttatt accattgacg
    10921 ataaaagcac ctgagccatt ccactccaga ctccccttta tttgactctt gactgcgtct
    10981 gttaagacac tctctgccaa tgcttgacta ggagcttcag gcgcttgttt tttctgacta
    11041 agattggttt tgggactatt aggtgccgac tgcatctgct tgatataata gctccctgta
    11101 gacaaaagca ataacactag cagtccgatt agtgtctgtc ttgttttttt gttcatattt
    11161 ctccttatct ttagaaaaag gctggttctc cagcctaatt tcctgtaaat ttatgaatca
    11221 attcctgcca ttttgctgga gacaggatag cccatggatc ttgacccttg cccaagattc
    11281 cataacctac cattagaccg attcctagag ccagagtacc taaaatcagt actatgatga
    11341 ctaaaagtaa acgcttgact acgtagcttg atttcttatt catcttcatc acttgcctca
    11401 atccgctgaa tcttagcacc tagctgagcc aacttctcat ggaaaccgta gtaacctcta
    11461 tccaagtgaa ccaatttacc gaccacagtt tctccctgtg ctaccaaacc tgtcaaaatc
    11521 aaggctgcac tggcacgaag gtcagttgaa agaacttctg ctccctgcaa aggctgtcca
    11581 ccaacaatac gagctgtatc acggataatc tcagaatgca agcccatgcg gcgcatctct
    11641 tctaggtgtt ggaaacgatt ttcgaaaact gtctccacca tggttgattc gccttttgca
    11701 actgtcatca aggctgtaaa ttgagcctgc atatctgttg gaaatcctgg gtggggcaag
    11761 gttttcacat gaacagcttt tagattttct agttgagaac gaacacgaat tccttcgtct
    11821 tcttcaatta cttcaacacc catttcaagt aacttggcaa tcaagggacg gttgtgctcc
    11881 cagacagcgt ctcgaatcaa gacatcacca ccagtcatgg cagcagctac cataaaggtt
    11941 cctgcttcga tacggtcctg gactacattg tgagtcgtac catgaagttt ctcaacacca
    12001 gtaatggtta tagtctctgt accagcacct ttgaccttgg ctcccatttc attaaggaga
    12061 atggctaagt caacaatctc aggctcacgc gcagcattct caatcactgt caccccatca
    12121 gccagagtcg ctgccatcat caagttctgc gttgcaccaa cacttggaaa gtccatatag
    12181 atatgagcac catgcaagcg ttctgccttt gcttcgatgt aaccagctgt ctgactaatc
    12241 ttaaccccca tagcttccag acctttcaaa tgaagatcaa taggacggct accaatcgta
    12301 caaccacctg gcatggatac cttggcatga cccacacggg caaggattgg ccctaataca
    12361 acgatggagg cgcgcatctt actgacatac ttgtaagggg cttcctcagt gatgtcgcca
    12421 gtagcatcca ccttgacaag atgagcttcc tcatcaaagt caaccttggc attcaaacca
    12481 ccaactacct gattcataat aaagacatcc gacaaaatcg gaacattctg caagacggtc
    12541 tttccttcac ttgctagaat agtcgctgcc aacaagggta agactgcatt ttttgctccc
    12601 tcgatcgtca cgcttcctac cagacgatta tcgccacctt gaaccacaat tttatccata
    12661 ctcgtttcct ttactcttga ttttataata atcctctaag cgcttcttgc cacaaatgat
    12721 acaggctaat aaagaaagaa ctcaggatgt agcctatgac aatgctaaaa aaggctacta
    12781 ataaacgaac ttttcttgta ttctcatctg tcacttttaa aaccttctcc catctaacaa
    12841 gatcctttaa aaggtaaaaa ctcacataaa taaagagcat atgactactt agagtgaata
    12901 ataattgaat catttgacta ttataccatc ttctctgttt gtgcgctagt ttggaaactt
    12961 cactcaaaaa ttagggattg tttttcaagt aatcaatcgc atcttgtagg gttttaacgg
    13021 gcacgatttt catatccgtc ttgattgttt tagccgcttc tagggctgtt tggtagttgt
    13081 ttttcgcgtc cggatgcgcc ttttgttctt cttcgctaac agggttatca ggagtaaaga
    13141 aaatagcagc acctgcccta gccgaagcta caaccttctt atcaatacct ccaatgtctc
    13201 ccacattacc atcgcggtca atggtacctg taccggcaac aatacgacca ttacgaagat
    13261 ctgggtgagc tatttgagta tagatagcta gactaaacat gagaccagca cttggaccgc
    13321 caataccagc tgttgaaaag ctaattggga cattgctgat tacctctgta cggtcaatca
    13381 agccgattcc aattccattt ttgccatttt ccaaggtgat gatttttcct tctgcagact
    13441 tggtttgccc atcctcttca taggtgacct tgacggaatc ccctaatttt tgagaactga
    13501 cgtaatcaat caagtctttg gaactatcaa aggtctgatc attgactgct gtgactgtat
    13561 cagagatatt gagaatccct ttaaaggttg aattatccgt cacattcaaa acataaactc
    13621 caaagtactt gagttcgata tccttaccag ctgtttttag tccttgatac ttggccatat
    13681 tttgcgatgt ttgcatgtag aattgattga ttcgcataaa ttcaacatcg gaagaaccac
    13741 ctgtagtctc ctgagcacta cgaatatctg taaaaggtgt caaccaagca taaatcatat
    13801 gagctaaagt ggcatgttga acaccaaccg taacgaattg ataggcacca gcttccttat
    13861 cttctgtgtc atttacttta aggacttggc gaatatcttc cgaaccacct ggaacctcta
    13921 tataataagg caagggcact acaaatgcca agaaagtcac aatcaaggcc gcaatgacgt
    13981 ataagggcca tctaatcttt tttttcattt cttatttcct ccaaaatact ctcgggaaca
    14041 tagcaggcaa tatcctgacc aaacttcaaa agctctctaa cgcctgatga actgatatag
    14101 agatgttcag gtcgactatg taaataaata gtctctatat cagaagacag ctgatgattg
    14161 tagtaatcaa aactggcttc atattgcaaa tccgacgcat ttctcaagcc tcgcactaga
    14221 caagtagcac ccaatctttt tgcaacatcg accaccaatt catcatgaga agacacgact
    14281 ttaacatttc ccaaatgttt cacagccttt tctaaccccc gtttacgatt ttcaagaggg
    14341 agaaatcctt gtttgtgggg attaaaaaaa atacccacat aaagcttatc aaagagtctg
    14401 ctcgcccgtt caatcatatc cagatgccca tttgtcatcg gatcaaatga gcctgtgaat
    14461 aagccaatct tatctgacat agactgtcac cttactaatt ccataaatct tttccttcca
    14521 gatacccaga caggcaatct cttctggaag ttcaacggcc ttatccgtct cgcacacaac
    14581 cataacatct tcagaaaaaa gctctctctc agccattttt tcaatatctg ctacgatttg
    14641 ttccttggca tagggagggt ctaagaaaac gaggtcaaat tccccagata cctgttccaa
    14701 tgccctctct gcatccatct tgaggagttg aaattttcca acttccttgg tcatctggat
    14761 attttcagcc acgatggtct gagccttacg gtctcgctcc accaaaacag cactggacat
    14821 gccacgcgat actgcttcga tagataaacc accactacct gcataaaggt ccaagactcg
    14881 tcccccttca aagtagggac caatcatgtt aaaaatggct cccctaacct tatccgaagt
    14941 aggtcttgtc gtcttgcctt ctagtgtctt gaggggacgt cccccataga ttcctgatac
    15001 gattttcata ctgtttatta taccaaatta tagacaaaaa gagaaagaaa accgaacctt
    15061 gcggttcgat tctctacaaa atattttcgt aagtatcgcg gacttcttga ggccaaacac
    15121 ttgtttgcac ttctccgatg tgtctcttgc gaagtaggaa catggccata cgagattgtc
    15181 caattcctcc accgattgtc aatgggaata gaccattcaa caaagacttg tgccattcca
    15241 attccaagcg gtcttcatca cctgtaattt ccacctgacg tctaagagtt tcttcatcta
    15301 cacgaattcc catagaagac aactcaaagg ctccacctaa agactcattc cagacaagaa
    15361 tatcaccatt tagacccttg tagccattct cagactcgct tgtccagtca tcatagtctg
    15421 gtgcacgtcc atcgtgcggt ttaccatctg gcaactcgcc accgatacca atcaaaaaga
    15481 cggctccaaa ttctttacaa atcgcatttt cacgttcttt cggtgtcaag tctgggtagc
    15541 gttctaccaa ttcttctgta tggataaagg tgatttgttt tggcaagata gactcgatgt
    15601 catagcgggc ttcaacagct agctcagtca gacgaatagc cttataaatc ttctcaactg
    15661 tttcttttag ataaacgatg ttacgcttac catttgggat aaccttctcc cagtcccact
    15721 ggtcaacata aacagagtgg gttgcatcca aggaatcctc atctggacga agggctttca
    15781 tgtggacaaa gagaccctct ccttcaccaa agccaaaacg agccaaggtg tggcgtttcc
    15841 atttagcaag tgagtgcacc acttcataag tagcatcagg gatttggaga accttgaccg
    15901 atactggatt ttccacacca gacaggttgt cctgcattcc gtcaccgacc ttactcaaga
    15961 taggaccttg aacttcgaca acttctagct tatctttcaa atactgggta aaagtgtttt
    16021 tgacaaagga aatttcttct tgttgatgga taaaactttt cttcatagac aactcctcaa
    16081 aaatttaata aacgtattat acacctattt ccaagaaaag aaaagagaaa atttaaaaaa
    16141 aatcagtgtc actcaaaaca ctgatttcat agacctttta gtcattgcga ccaaagatac
    16201 gtagaatact taggaaaaga ttgataaaat caagatagat gctgagagcc attgacacca
    16261 cccagcctgt cgctacacga ccttgtgatt gttcataggc gaggcgaatc ttttggttat
    16321 cccaagcaat caaccctgaa aaaaccaaaa ccatggctac gctaatcata taatcaaaga
    16381 agccactagc caagaaaata ttgactacca tagcaatcag taaaccaatg agagctgcca
    16441 tcattgctcg accaatccca ctcaagtctt tcttggtaaa aataccaact gccgccatga
    16501 caaagaagag aagggcgctc gatacaaagg cagataaaac tgtacccgga gtatagaaag
    16561 ccaccacaaa actaagggta aagccgttta aaacggagta aagtaaaaat actggaagag
    16621 cagctggact atttcttgaa gccatgctac tggcaacgaa aaccaaagcc aactccgcaa
    16681 aagtagcaat ggtcaaccag agacgcccct gcatcaaaaa gtaaaccaac tgagactgaa
    16741 agaccgtcaa cataaggcct gataccaaag cagatagtcc gattcccaga ccaacaaagg
    16801 cataaacctt agcgtaaaat tgattgagac ctgcacggtc atgtataata gtgtgattca
    16861 tccatttctc cttttctgtg aacatctttc cttgattata acaaaaaaag ttaaaattag
    16921 cttaaatgga agattaaaat accggctgca accgcaacat tcaaactctc ggcctgcccc
    16981 ttcatgctaa tatggaccaa ctggtctgca ctttcagcca taaggggact aattccttga
    17041 ccctcatttc ccataactag tacaaaattt tctatagaag gcagttctct gtaatcaaca
    17101 gaatctttag atagggttgt tgctagcact gggatagcta cctttttagt ttcgtcaaga
    17161 agcgcttgac tagtcatccg gtaaatgggc agatggaaat gactaccttg catggaacgt
    17221 agggttttga gactgtagat gtctgccgac ttatctgaaa caatcactcc tgtaaaacct
    17281 gctgcatccg cagttcgaat gatagttcct acattaccag gatcttgcac atcttccaaa
    17341 aacaagaact tgccctgact caaatcagct tgtcctactt cttctttttg aaccacggca
    17401 acaattccct gtggagtctg agaatccgcc aaatctagca aaatatcctc tgacacccag
    17461 acagtttgcg aaaaagtagc taactgatct cgataatttt ctagggcaaa gattttctca
    17521 atcgtcactc cagcttggac agcttcttca aacaagtgcc agccttcaat caagtaggca
    17581 gacttgcggt attttttttg gcgtaatttc ttggcatttt ttaccacaga attggctttt
    17641 gaggttataa tagtcataga aatattataa cacaatcaaa ggggtttggt atgcaaaagg
    17701 ttagaatgat tgcccaaggt agggtacagg gagtcggctt tcgttggggt gtttacagct
    17761 tggcacttga aattggtggc atcacaggtc gagtatggaa taacgacgat ggcacagtgg
    17821 aaatcttagc ccaagcagac tcatctgcta tcatggcaaa atttatccaa gaaatccgaa
    17881 aaggaccgac acctttttca aaagtaagct acttagatgt caaactaagc aactttcctc
    17941 cctactctga ctttaaaatc gcaaattagg tctccagaac tattgtatat tttgtaaaaa
    18001 aacagtagaa tagaaaggta taatttttaa agaaggaata aaaacagtga aatctattaa
    18061 acgttttgca ctctcagcta tgggagcggc tatgttgctt gtcttgactg gttgtgtcaa
    18121 tgtcgataaa accacaggtc agccaacagg atttatttgg aataccatcg gagcccctat
    18181 ggctgaagct atcaagtact tcgctactga taaaggtcta ggctttggtg tcgctatcat
    18241 catcgtaacc attatcgtgc gcttgattat cttgccactt ggtatctacc aatcatggaa
    18301 ggcaacgctt cactctgaaa agatgaacgc cctcaagcac gtccttgagc cacaccaaac
    18361 gcgtctcaaa gaagcgacta ctcaagaaga aaaactcgaa gcccaacaag ctctctttgc
    18421 tgctcaaaaa gagcacggta tcagcatgtt tggcggtgta ggatgtttcc ctatcctcct
    18481 tcaaatgcct ttcttctctg ctatctactt tgctgcccaa catactgaag gggttgctca
    18541 agcaagctac ctaggcattc ctctaggttc tccaagtatg attttggttg cctgtgctgg
    18601 tgtcctttac tatcttcaat cgctcctttc acttcacgga gtagaagatg aaatgcaaag
    18661 agaacaaatc aagaaaatga tttacatgag cccactcatg atcgtcgtct tctccctctt
    18721 ctcaccagct agtgtcacac tttactgggt tgtcggtggt ttcatgatga ttctccaaca
    18781 gtttatcgtc aactatatcg ttcgtccaaa acttcgcaaa aaagtccgtg aagaactagc
    18841 caagaaccca ccaaaagcaa gtgctttctc taaaccaagt ggacgaaaag acgttacccc
    18901 tgaacaacca actgctatca caagcaagaa aaaacacaaa aatcgtaacg ctggaaaaca
    18961 acgttcgaga taagaagaaa aaggcttagc tgatttgcta agtctttttg caaatcaaaa
    19021 gcccgatgtt tccatcaggc ttcttttcta tccatgtaca cgtttcatat aatcttgata
    19081 actttcggta tccatgagtt ttttagcgtt cttgacgcga tctgctgttg gtggtttgac
    19141 tccttcgagg gaatatggaa ttccaagttc acgccacttg aactcaccca tggtgtgata
    19201 aggtagaatt tcaaacttat caacattttt gagggtcttg acgaacttac caagttcaat
    19261 caagtcatca tctctgtctg tcaatcctgg aactagcacg tggcgaatcc agacaggttt
    19321 tccaatatct gatagatact gggcacaagc caagatattt ttattagttt ggctagtgac
    19381 aatcttgtgc tgttcttcgt tgatttcctt gatatccaaa agaaccaagt cagtgacagc
    19441 catgagtttg tcaaacttct caaggtaacg tggtttatta cggaaaggaa gagcacaggt
    19501 atccaaggta cagtggattc cttgttcctt agccttggtg aagagagcaa tcaggaaatc
    19561 aatctgcaag agagcttctc ctccactgac tgtaatccca cccttatttc cccagaaacc
    19621 acggtagcgc aaggcctctg tcaagacatc atctaccgtc cgttcacgtg acttattgga
    19681 ctccatagcc caagtgtctg ggttgtggca atactggcaa cgcatgtgac agccctgcaa
    19741 aaagacaata aagcgaatac caggcccatc tactgaccca aagctttctg tcgaatgcac
    19801 cattcctgtc acttgtccat aatcaattgt ttcttcagac atatcgttac cttccttgaa
    19861 aacgttttat agttttatta tatcacgact tgaaggaaaa acaagatttt tgcctatttt
    19921 ttagaaagca atctgttttt caatttcatt ttcaatctat tatcagatta ttcttcaaaa
    19981 tcgaagccat ataaggttgc aaaatagtcc tcagggcgct ctgcacggcg gatttgacgg
    20041 gctttacctt cttcggtata gaggatttcc gcagaacgta atttggcatt atactggtag
    20101 cccattgaaa atccgtgggc acctgtatca tgaattacca gcaaatcacc gatttctgta
    20161 tgaggcagtt cgcgattgac tgcaaattta tcattgtttt cacagagtga accaaccaca
    20221 tctaccgctt cagctggtcc atctggatgg gtcacgttgc taatatgatg gtaagctccg
    20281 tacatagctg gacgcatgag gttgactgct gaggcatcca cacctagata ggtacggtag
    20341 gtttccttct tatgagtcac tcttgtgact agagcaccgt gaggtgccag cataaaacga
    20401 cccaattcgg tgaaaatctt gacctgacca agacctgctg acgtaagaac ttcttcatac
    20461 accttacgaa ctccctcacc aatcaaggcg atatcgttcg gctcctggtc tggacgataa
    20521 ttaacaccaa taccgccaga aagattgata aagtctaacg aaatgcccaa cttttccttg
    20581 atttcaacag ccagttcaaa gagctgacga gccaactctg gataatagag atgggtcacg
    20641 gtattggacg ctaggaagga gtgaagccca aaagtcttgg ctcctttttc cttcaagata
    20701 gcaaaggctt caaagagctg gtccttggtc atgccaaact tagcctcctc aggattgtcc
    20761 ataatgtctg tccccagttc aaaaacgcct ccaggattat aacgacaaga gatgatttct
    20821 ggaatgcctg ctactctctc cagatgttca atatcttcaa aggcatccaa gttaatggtc
    20881 gcacccaatt cacgtgcata ggcgtattcc ttgtctggcg tgttgttgga agagaacata
    20941 atctcagaac ccagaaagtc cagtttatgg ctcatcaaaa gctctacata actagagcag
    21001 tccacaccac aaccttcttc ttggagaatt ttcaaaatag ctggagttgg agtagcctta
    21061 actgcaaaat attccttaaa gcccttgttc cacgaaaaag cttggttgac agctcttgcc
    21121 ttctcacgaa tccccttctc atcatacaag tgaaagggag tcgggaactc ggcaacaatc
    21181 gcttctaact cttctctatt gataaatggt gttttcataa gcttccttct attctgtttg
    21241 caaagaaagg ctgggacaat cgttccaacc tttagttcta tctcttattt atcttcgtca
    21301 aagatattgc caacctcaac atcgatggct tggttcttga catcaatatt agttaccttc
    21361 aagagtgact tgtagtcaaa ctgcttttca cgttcttctt gggcattgtc cgcaaagacc
    21421 gctacacctg ccagttctga gtcgaactcg cgcaagagac taatcatacc gttgaccgtt
    21481 ccgccacctt tcaagaagtc atccacaatc aagacacggc tgcctgcctt aagactacgt
    21541 tttgaaagga acattttctc gatacggtca ccacttgaac ctgaaacata gttgacgcta
    21601 acagttgaac cttcggtaat tttcaggtca cggcgcacaa tgacaaaaga gacattgagg
    21661 acattggcaa ctgcatttgc aagtggcaca cccttagttg ctacggtcat aaccgcgtca
    21721 attttttggt ccataaagct tttggcaata atacgaccaa tatttttcaa gatggctggt
    21781 gtgctaagca aatcagacag atagatataa ccacctggca agatacggtc actttctgac
    21841 aacttggtac gcaagtcttc aaccatttcc ttagcatcct gactcgagat agacggtgtg
    21901 aagatgacac ctccgccagc cccagtcact gtctggatat gaccgatttc aatttcctca
    21961 aaggcgcgtt tgataatgac gatatcttct gagatggatg atttagcaga ctcatacttt
    22021 tcagcaaaag tattgagact agttagttta taaggattat taatcaaata gttggaaatg
    22081 acaaccatcc gatcacttct tcttaatttc atttctattt cctattccat ttttattcaa
    22141 aaaatcaaaa agcaaactag aaagctggtc gctggtggtt caaaacactg ctttgagatt
    22201 gtcgatagaa ctgacaaacc ctgtaatata cctgcatata tacatacgac aaggcgatac
    22261 taccctagtt tgaagagatt ttcgaagagt attcattttt gtcttttact tattatacca
    22321 tattcacata aaaaaacgaa cattcttatc ctaaaaaatg ctcatttttc ttaaattatt
    22381 aatctaaatc tggtttatag aaggaacgat tatccatagc gaagatttta ttggacatct
    22441 ctcctttatc caccaaagcc agagctgttg acatcatcat catgcttgca tccagattgt
    22501 caatcatatg gataatctct gcctccataa tacgtggacg gactggactt ccatattcaa
    22561 gcaagccgtg gtgactgagg atgacatgac gaagcaaaac gacttcttcc ttggtatcat
    22621 cgatgccgag ttccataact gtcttggtaa tttcgctatc aatgagagcg atatgtccaa
    22681 gaagattgcc tctgaccgtg tactcggtct ggtctggtcc cgtcaactcg atgaccttag
    22741 ccaagtcgtg caacataatc cccgcataga gcagactctt attgagctga ggataaactt
    22801 cgctaatagc gtctgccaaa cgtaccatgg tcgccgtatg ataagccaac cccgtttcaa
    22861 aggcatggtg gttggtcttg gcagctggat aggaatagaa ttccttatca tacttggtgt
    22921 agagatttcg gacaatccgt tgccagacag gattttcaat tttgaaaatc atttgcgaca
    22981 tgtagtcacg aatttccttg acatcaactg gtgacttgac cttgaaatcg gctgggtcat
    23041 tgggttcacc agcttgaggc aggcggagag taatttgatt gacttgaggg gtattgttat
    23101 aaacttctcg gcgtcctttc atgtggacaa ccttacctgc ggtaaaggcc tcaatgttat
    23161 gaggttgggc atcccagagc ttcccatcaa tctcgccact atcatcttgg aaggtaaagg
    23221 ctaggtagtt tttcccagct cgagtttgcc tcaggtcagc tgatttgatt aggtaaaagc
    23281 cttcaaatag ctcatctttt ttcatgtgac taatcttcat attcttcctc attttcttga
    23341 aaatggagta gatcaagcgc aggctcgcct tctgacaact caatgtgacg gagcgtccgc
    23401 tcgatagcta tggtacgacg gtttaatagt tcatcaatat tgccagaggc atgttggaga
    23461 tgtttttgtg ccttaaccag aatgccacca aacttgccaa actcggtctt gacactggca
    23521 agagtcttgc tgatatggtc ggcactcttt tggatattaa gggtcttgaa accaactgat
    23581 agggagttaa gaagggctga tagggtactt ggtcctgcaa cgataatctg ttcttcccgt
    23641 ctcaaattat caaagaagac cggattgcgg acgatttctg agtagagacc ttctgtcgga
    23701 acaaacaaaa ctccaaaatt ggtcgtccga ggtggtgcta tgtacttgtt cctaatatcc
    23761 ctagcaaagc gcttgacgct tgctaggagt gacttacgac agcgttcaat ctcatccttg
    23821 tcacctgtct cataggcttc ttccaagcgg taataatctg ccagtggaaa cttagagtca
    23881 attggcagat agacgtattc ttggtcgcct tgtccgggta acttgatggc atactccact
    23941 cgttcactag agttttcaac cgttgcgtat tctcgttcgt actgggcagg tgtcatgatg
    24001 tcttcaataa tttgccccag ttgcaattct cccagaatcc ctcgcgtctt ggttccagag
    24061 agaaccttgt taagagctcc gacatcacgg gcaactgtct gcatttctcc aaggccacga
    24121 ttgacagact ccagttgttt agaaactgtc tcaaaggaag cctgtaagcg tgtctgcaag
    24181 gtcttttcta gtttttcctc gaccgtctgg cgcatttgtt ccaaacgttg ctcatttgat
    24241 tcctgcaagg cttggagacg ttggtctgtc ttgtctctag tttggaggag attatctgtc
    24301 atttcttgac ggacttgggt cagaccttgg tgcaattcaa tccgcacttc ttgcaaacgg
    24361 tcgctgacaa ccacttccaa atctttttgg tctaactggc tggcttgtct ggcttggtca
    24421 aagcggtaat ccaactggtc tgacaaatga tctgcctgat cctccaagct cttacttaag
    24481 tgtttctcct gcctatcctg cctttgccaa atcagaaaga gaccagctag gttggcaatt
    24541 aataatagaa taagtaaact ttccatccta cctcctgtcc ttgctatgca gtacgaccac
    24601 ataaccatct gggcaagtca ccgacacttc cctatctata tattcgttag aagcgtacac
    24661 ttttttaaag aaaaaattct cctctgtcaa ctcatactta gctccaagaa tagtcagctg
    24721 gctatcccga actggcataa aggctagata gtcgtagtct gaacgaggtt ctagctgact
    24781 ggtcccttct gaacaatagg caatcaaatt ttgcccatcc tcaatttcta tttgacgcat
    24841 atagggtgcc aacttaggat tgctaggtag aaagacattg gccaacatat ggtcaatacg
    24901 gccacccaag gcaccgaaaa tagtgacctg agcctgagga ttttgttcaa agatggttaa
    24961 gagagccaat tccagatctg tatcatcctt ttctggtcgg gcttggacaa aatactgggc
    25021 acgtttttga atcacctgtc gctcttctgc agtcacagaa tcaaaatctc cgactgctag
    25081 agcaagaggt aagtcttctt ccaagaccca gagcgagcct cgatccaccc caacaaaagc
    25141 atcaaaatct gtccgataat gaccgcggtt tccgcctgca aaaactgcaa ccctagtcca
    25201 gttgttttct gagagtttgt actcgctcat tgacttctcc cttaaagaca taggaacctg
    25261 ctacaaaaac agtcgcacca gcttctttgg cttgagcaat agtttggtca tcaatcccac
    25321 catccacttc gatttcaaag tttaaacctt tttcctgacg aagagcaacc aactcacgaa
    25381 ccttatccat ggtttctggc agaaaggctt gcccaccaaa acctggatta accgtcatga
    25441 ctaaaacttg gtcaactaga tgaaggacgt gcttgatggc ttcaactggt gtgccaggat
    25501 tgataacgac tgaaggctta actccgagtg aacgaatttt ttggagggcg ccatgaatat
    25561 gaggcgttgc ttctacatgg atactgatga tgtctgcacc tgcacgcgca aaatcttcca
    25621 gatgatgctc agggtttgac accatcaagt ggcaatcgaa aaccatctta ctatgaggac
    25681 gaaggctctc gaccacacct gcaccaaaac tgatttgcgg tacaaaatga ctgtccatga
    25741 tatcgatatg ggcatattct gccccagttg cttctagacg tttgatttca cgttcaaagt
    25801 tggcataatc tgctgccaga attgacggag caatcttgta ttgagacata ggtttctcct
    25861 tattttggaa tttttttgct gactttttta taggtttctc tacgattttc aatttcgctg
    25921 aggaattgca ggtagttgtc aaaacggaag gtggcaatgg tcccctcttc tacagctggc
    25981 ttgacggcac aagacggctc atgggtatgg gtacaagtac gaaacttaca atctcggctg
    26041 acagtagcaa tctctggaaa ggcctgattg aggtcttcag cccttgatac ttcatagtcc
    26101 agagatgaaa agcctggtgt gtctgcgatt ttacccccat tgagattgta aaaactaaca
    26161 gctcgagtag tatggcgccc acgacccaga ctgtctgaga tttctcctgt ttcaagattg
    26221 agatctggtg cgattttgtt gagaagagtt gacttcccaa cacctgtctg ccccataaaa
    26281 accgtaacct tgcctgttaa caaaggcagg agttcctctt tactggtcac aaagtcatag
    26341 ccgatgtcac cataggtctg ctggtaaaaa tccagttctc ccctatcttc caacaaatcc
    26401 attttggaaa tatagacaat gggatggatg cccttgtgct ccaaaagaac caagaaacga
    26461 tccagcaaat tgctgttaaa atcaggttcc ttgacggaca tgattactac agcttgatcg
    26521 atattgacaa taggcggacg aaccagactg tttttccgtt cgtgaatttt gaggatatag
    26581 ccttctgaat tttcctcggc agagaaatct acccagtccc caacataagg ggtatggcct
    26641 tttttacgga aattcccacg cgcgcgtgtt tgataaacct ggccatcact ctccacatag
    26701 tagaaacctg ccaaggcttt aatgatttgt ccctgcatct tagagtcctt gccctttaag
    26761 tgcgtctgct agaccggcaa attctgccaa gctgagagct tccccacgca cacttggtga
    26821 caagcctgcc tggtccaaag ccttggtcag cttgtccttg acctcttcag tcttaccaaa
    26881 gtaacctgtc aagttattcc acaaggtctt gcggcgatgg gtaaaactag ccttggaaac
    26941 cttaaagaaa aagttctcat cttctactgc tacggctggc tctggacgac gcaccatttt
    27001 caagatggct gaatccacat ttggcgctgg cacaaagacc gtacgaggca cgataaaggc
    27061 aaccttggct gtcatgtaat actgcacggc gatagacaag ctaccgtaag ccttggtgtt
    27121 aggctgggct gaaatgcggt ccgctacttc tttctgcatc atgaccacaa actcacaaaa
    27181 aggaatgcca ctctcaatca agtgcatgag aataggcgtc gtgatgtagt aaggcaaatt
    27241 agccactacc ttgattggca ggtcaggatt tttaaaattc tggatatgtt gcgccaaatc
    27301 aaccttgaga atatcttcgt taactacggt cacattatca aaatcacgca gagtatctgc
    27361 caaaattggc accaaacggt ggtcaatctc aaaagccatg acttgggctg cacgctcagc
    27421 caaaaattct gtcaaggcac caatacctgg cccgatttcg atgacattga cctgatcatc
    27481 aatttcagcc gtatccacaa ttttttgaag gatattggta tccgtcaaga aattttgccc
    27541 aaaggacttt ttaaaggtaa aaccgtgacg ctccaacact gccttggtca cgctataatc
    27601 tgcaattctc atctattctc ttttctatta tctgttacta ctattgtaac acattcccct
    27661 tgtattttgg gaatgaaaat cataccaaaa aactatcttt cctcatgaag aaagatagtc
    27721 ttggaaatct ttaaagaggg acttagcgct tctctcgaaa catacgttca tactcttcga
    27781 aaatctcttc aaatcacgtc agcttcacct tgccgtactc aagtacagcc tgcggctagc
    27841 ttcctagttt gctctttgat tttcattgag tatcaaaaaa atcagcttct tgataacttc
    27901 gattttcggg tagggtcaaa tccacatcct cctccacatc atccactgtc agttcaaccg
    27961 tttgaggtaa atttttcaaa cgataatgct tttgccttta cccttcaata ctagcaaaat
    28021 caagcaaata gccatgcact cggatttgaa ccaaaaggag atgattggtc atattttcca
    28081 tgatgaggat atcttctaaa ccacctctgg cactaggata atcagatacc ctgcgaatac
    28141 ggattttttc acacatgagc aagtttcctc atatcaatga tttcatcagc cttattccag
    28201 actaggggat tatgggtcgc aatgatgata attcgatttt catctttcaa atccaccaag
    28261 agattcataa cctcctctga attttcagga tcaagagctg ctgttggttc atctgccaaa
    28321 atcaagggtg gatttttcaa aatagtctta gcaagggcaa ctcgttgggc ctctccccca
    28381 gataaagtat agattttttg ttctaaatcc aaatacccta gattaacttt ttctaaagcc
    28441 cccacttgcc tttccaaacg ttctactttt gagattttct gaccaacaaa acccaaatcc
    28501 aaattttctt tgattgattg gttttctaag aggccaaaat tttgaaagag atagcccatc
    28561 tgatctcgaa aatactcacg agtgggaatg gtttttaaat ctttcccctg atagagaacc
    28621 cttcctccat ctatcttttc cagctttccc aagatattca gcagcgtcgt ctttccgctt
    28681 ccactctttc cgattaaggc ataaaccttg cccttctcca gcttgagatt caaaccagag
    28741 aaaatcgcgc ggtcattaaa tttcttttcc aatccttgaa tatcaatcat cttattttcc
    28801 tttcaatact gccatgctac cagcttcttc ttttttgtcc tgccttacta aaatcaagag
    28861 ggcgttaagc gtaaacaaag ctacaactag agcgctaatc aaaccatctc ttgtcaaaat
    28921 actagatagg actaggccaa gcaagagaac tcctccttgc gccagtaaat acttgccatg
    28981 aagctcatag attgtcatac cagcaagacg tttaatcata agctcccgtc tgaactgctc
    29041 aaaatagaga agattcatgg aatcaaataa caagatagcc gtagacaggg tcaaaatcgt
    29101 cccaataagg agagagtaaa actccacccg tttgtcattt agtaccttgg caaaaaatag
    29161 ctggcttttt accaagtaag aaaccttgtg atacagacct tgctctttta gagctgtgat
    29221 gctgtctcca tatcgatcta gtttcaaggc actattagcc gtagttcccc acaacatccc
    29281 tgcaacaggt cttgttccag tcgcttgcgg cgttagaact acaataatcg gatcttttag
    29341 gtactggcgt gttgtcttgt acccatcatt atagaggaaa cgttcctgtc ctgtttctgt
    29401 aaaagctagc cttacctgtg ggaggtagtg tttctgactc gtcacttcta cactttcaga
    29461 tgaaaagttt tgcaggtaat ctgtaaacaa tccttggtag taggcagact gctctcgcaa
    29521 gctctcaggc aagatcagcc caaactctcc ctcagacaag ttttgcatct tgtccataaa
    29581 ctctgaagaa acggtaatct tttcctctat cagatagcgc ggtgagacat agataacatt
    29641 ccctgacggt gtgtagtcac tgagacgatt gccatctagg tccacttcta ctccatctga
    29701 gaaattgtca acattgctca taatataaaa agagtctgta ttggctaacc gttcttcagt
    29761 aaatgtctgc cactcacgat tatccttacg cattccttct tcatcggcaa atgcactaga
    29821 ccaaccaaag gagagacggt aacggtctga ggactggctc catttatcgc ttgccttctc
    29881 catctcctgc atttcacggt agttggggag gagagctgtc gcactcaagc cgaccaccaa
    29941 tacagctaag agttgcccca ccatcattaa tgtcatcata cgtttgagag ggagtttccc
    30001 tttcaataga tccaccagac tattttcctg taaaccaagt agatagacga cacttagtaa
    30061 ggtagaaatc cctgccaggg tcaatccata aagtagaaga gcaatgatga ccagttgcac
    30121 cgttgccata aacaaggcac cttgatacca gagaatcccc aatcccaaaa gactggatac
    30181 cagcactgag cagataaact gtctcacatc ttctaacact ggtctgagag caactccaaa
    30241 caagctctca ccagctatta agcgaatccc tgcctgacga agggatttga tccgataaat
    30301 cagggtcaga ctcataaagg tcagcagaaa aatagctaaa ctcagtagca ccataggggt
    30361 ggccgccaat agtaagacta tcgaaaatgg atcttctcca ttcgaaacaa agccttgata
    30421 gccaagttct ttcaaggtgg tctgtaagct cactccatcc aaacttcctg acacaatcaa
    30481 gtaagaccct aataaatcac tcgtttctgc actctccttg gaggaaatgg tcagaccttc
    30541 tggaagctct ccctcaccat aagtggcata ggtaaagagt gtttctccag cttcatttgg
    30601 ctcaacgatt cgacgggcga tgagactatt atgctctacc gccaagtgac tcaaaacctc
    30661 tgtcacctct tcgcgactag ctactggttg atttatcatc attcctgtca catcaacaga
    30721 aggatagtta ccaaataaaa tctcttctcg atggtcaacg gaagttatca ccatataaag
    30781 cgataccaat accattgaaa tcaaaataaa taaacgtttc atcatttcct ccaaaaagtt
    30841 acttggtcac ttagtgagaa ggtgaccaca gtcactgcga tgttaagaag cttattaagt
    30901 tctttctgct agcaacatct tcaaatacat tatatccgtt ttttcaagaa ctatcaagga
    30961 catcaatgtc cttaaaatct aaccatttcg tgtcattttt agcttatatc taaaaaacag
    31021 ccctaaagac gaactttaga actgttttga tgcgagaaca aggacatttt tgtcactgat
    31081 tcactatgtt tataaatatt ttttgacatc ctcttgccat tcctctgtca atttctttac
    31141 tagaaattca tctccaatta gctgcgcaaa tgttttagaa gactgataaa atgattcagc
    31201 taaactaaaa tctctttctt ctcttaatgc ttgcttccac aaaaacataa atacaagagg
    31261 tttcttttga taatcttgag ttttatccat aatttcttgt aaacacgata gataatcatt
    31321 aaaacaatca gttactccta ccttttcaag acaacacatt cctgaaataa gagtatctct
    31381 aattataaag aagttttcta atttagttac ctttttctgt tccaaaagaa agagcattag
    31441 ctcttcgatt tgatttataa atttggcaag atcttttcta atgagcattt gataaaaaaa
    31501 caaacgaatc aacaacatat catttgttga ataaacttct ttttctatga tctgaggcat
    31561 atgttcctca agtattaact ctgcatattc aggccttcta gttctagaag tatccaaaca
    31621 cgcctgcaat acttcacatg ctaattgttc ttctttagga agattatcaa agtatgtttt
    31681 ataaatctct tccaaacacg cttccttctt atcgtactcc tcttctttac catagattgg
    31741 ttcacgtaaa atctgatatt tcaattctaa ataagcagga ggaagagctg aaaaatccgg
    31801 cataaggcta taaactggaa ctcctagccg gcgagcaata tagtctaact tgggcaaact
    31861 cggttgcgaa gctccacttt caattcgcga cagttgacga actgtcagtt cttgctcatc
    31921 tccacaaaaa tctggacgac taattccttt ttctatccga atctctttaa ttttttgacc
    31981 aattaacatc agacgcctcc tcttctacct gcttcgaaaa atactgtgaa acttaaaaaa
    32041 ttgtaacggc tacatctttc ctactcataa gatttcatag cttcttccac ttctgccaaa
    32101 gtaaccccaa acaactctag gcgtttgagg agttgcttgc cgttagaata gccgattcgg
    32161 agagtctctc cgagatattc tctacgctta cggctgtctg cccctgctag aaaaccaagg
    32221 cgaatcaagt cgctacgact aatgtcaaac tgactctcat gttcaaattg ttctgtcacc
    32281 tgagctagag ccgttttcag gtcttcatag ctggcatgct caattcccag agaacgcccc
    32341 ttggtcttgg acttgggaac agcttcatct cgcttgagaa aggcatgctg aactgttgga
    32401 atgaccgtca tgatcatgcg ccgaatccgt tccccattaa aatctgggtc tgtaaagaca
    32461 atgactccat gacgttggtg caggcgctga atccgctcta tatcctgatc attgatggca
    32521 gaacctcgag tctcataggt ctccacatcg aaataacgtt tgagattgac cgtatcatcg
    32581 cgaccttcaa ccacgataac ttgagaaatt ttctctttca ttacttgctg tccaatccaa
    32641 aaattcgttc tgcatttgca gtcgttgcta ccgccagctc ttctgtcgtc ataccacgca
    32701 agtcagcgat aaagtcgacc acatagcgag tataggctgt tttattttca cgaccacgct
    32761 tgggtacagg tgctaagtaa ggcgcatctg tttccaccaa catcttgtcc aaaggtaact
    32821 ctttagctgc ttcttggagg tcagttgcct tcttgaaagt caccactcct gagaaggaaa
    32881 tggtcatacc aagatccaca aacttctctg cccactcaag cgtccctgaa aatgaatgca
    32941 tgataccacc acgaggacca acgccctcac tcttgataat ctcataggta tcttccagcg
    33001 catcacgggt atggacaaca aaaggcaaat ccaagtcctt agatagctga atctgacggc
    33061 gaaaaacctg ctcctgcacc tctttgggcg ctgtcatcca atggtagtct aagccaattt
    33121 cacctaaagc cacaaccttg gaatgtttta acttatccaa caagtaagcc tcaacttcct
    33181 ctgtataagt accagcttct gtaggatgcc aaccaatagt cgcatagagc tgctcatact
    33241 catctaccaa ctccaaggca tgctcaatcg tcggtttatc aaaaccaaca atattcatct
    33301 gtgtcacacc catctcagca gccaaggcaa tttcttctgt ctcacgacct gcaaattctt
    33361 ctacattcaa gtgtgtatgt gtatcaaaaa tcatctcttc taacctcgtt ttctatcttc
    33421 tattatacca aaaaagagga ggggcaccta atttttcggt ttcccctcct ctcttcaata
    33481 gagagctatt ctgctatctt ttctatccga tattgcccat ctcctattcc acagttagag
    33541 acagaagaga ttggctacat tgatagaata ttcctattca gctaaagtat gtgattatct
    33601 tgggttctca cttttctcac ccatcactag gattgatgac ttctcaaaat cccccttata
    33661 atctaacttt aagtagagaa tagaattttc tagctgttct attttctacg tctagtcttt
    33721 cttcctagca aagcaaaacc acttgttgcc accactagac caagactagc aagtgctgca
    33781 cttgatgctg aacctgtgtt aggtaattgt ttgccagcag gtgactggta ggtaacatct
    33841 tgcattgttc ctgcagttgc ttgcggtgca cttgttgtct taggagcagc attaggagcc
    33901 actggtctct taggaactgt aaccatggca atggcttgca ctccaatcgt ttctgcttcc
    33961 ttcacatctt ccatagtttt cgcattttca atctctttga gagctgcttg tttttctgct
    34021 tccactcttg ctaaaagttc tgctttttct ttatcagaaa gcggtgcgcc tttgatttca
    34081 tcctgcttgt tcttggctgc tttttcaatg gcatttttag ctgattcttt ttcagtagct
    34141 aattgcttag aagcttgtga ttgagctgag ttttgtgaac ctgtgtgagc tgatccaccc
    34201 gcctggctac caccgccttc attcgcatta gcatctgagc ttccagtatt gcctccctta
    34261 tctccacctg gtgtagtttc tagcttatac tctggtgtag cttcttgagt tacagattca
    34321 cctttcgcag attgttgaac tgtatctttt cctagaatcg tcactactga accatctggg
    34381 aatgtgattg ttgctgtacc atctccagct acattgattg tcgctccgtc taatgctgaa
    34441 ccatttgctt gtaaaatagc aaccttaact ttttcttcat ctgttaaatg agttatatct
    34501 tttacatcta ctctttgagc aggtgcttta acagttgatt tttcagcagt cggtttagtg
    34561 ctatcttgtg cttttggatc ttgtgctacc aattgatttc ctggaattgt aatagctgaa
    34621 ccatctttaa agacgattgt cgctgtgcca tctccagcta cttcaatttt atagtaaaat
    34681 aaaataataa caggacaaat caatcaggac aatcaaatcg atttctaaca atgttttaga
    34741 aacagaggtg tactattcta gtttcaatct actgtaacat cgcttccgaa tccatttgca
    34801 tctactgaac ttttttacaa aaataaaaat cagtagactc gagttcctac tgatttttga
    34861 tagaattcgt ttggattatg cagccaaaac tttgcgtcct ttacgacgac gagctgccaa
    34921 tacgcgacga ccatttttag ttgacatacg gttacggaat ccgtgtttgc gcgcacgacg
    34981 aagtttactt ggttgataag tacgtttcac gatgaatacc tcctcataga ttttgtattc
    35041 gtttagccgg ctataaagtg atcagttact aaacatactt tactattcta tctgattctt
    35101 tcacttttgt caatagctct aggcaaatgt ttccagcttt ttcgaatgca agccctatct
    35161 acgatactgt ttcaagaaac gagtcatctt ttcttggatt tgggctaact catcaacacg
    35221 aggaaggtaa acgatacgga agtggtctgg ttcctgccag ttaaagcctc gaccatgaac
    35281 caagagaacc ttttcctgct tcaagaaatc aaggacaaac tgctcatcat catcgatacg
    35341 gtacatattg cggtcgattt ttgggaagat atagagcccc gccttgggtt taacggcaga
    35401 caaacctgga atatcttgaa tggcattata gatgaaattt ctttgctcgt agattcgtcc
    35461 accaggaaga agcaattcat cgactgattg gtgacccccc aaggaagttt gtacgacttg
    35521 ttgggccaaa acgttagagc aaaggcgcat attggacagc atattgagcc cttcgatata
    35581 gcccttaaca tgagtcttag ggccagacaa gaccatccaa cccacacgga aacctgctat
    35641 gcggtgggat tttgacagac cattcatgct gacacagaag acatctgatg ccaagctcgc
    35701 cacaggcgta tgcacatgtc cgtccattac catgcggtca tagatttcat ccgcaaagat
    35761 aatcaaatcg ttttgacggg caatctcaat aatctccagc aagagttcct taggataaag
    35821 ggctccagtt gggttatttg gattgataag gacaattgcc ttggtattgg aagtgatttt
    35881 tgacttgata tcgtcaatat ctgggtacca ttccacagct tcatcacaga tatagtgaac
    35941 ggcatttcct ccagctaggc tgacagccgc cgtccataga ggatagtctg gcattggcac
    36001 taagacctca tccccattgt ccaaaagccc ctgcatggac atgacaatca actcactgac
    36061 accatttcca aggtaaatat catcaatatc tacgtttggg aatttcttca gctggcaata
    36121 ctgcatgatg gccttacggg ctgagaaaat ccctttggag ttagaatagc cttcactatc
    36181 acgcgcattc ataatcaagt catgaatgac ctcgtctggc gctgtaaagc caaattctgc
    36241 tggatttcct gtattcagac gtaaaatctt ttctccattc gctcgcatcc gcatggcttc
    36301 ttccaataca ggaccacgga tatcataagc aacatgctct aacttactag acttgttata
    36361 ttctttcatc ttgtttcctc aattcatcag gatagtaaca ttataccact agaaagagaa
    36421 tgcgacaagt ttactgaaat gtgttactag tttcgtatag aagaaaaacc ttgctaaagg
    36481 cgaggttaag aacgtagaga tagcaggtat tttgcaaaga cacatcacat accaaaagat
    36541 aaacctagaa aaagcaagcc tagaaaggag tcataaagaa gattaaacat gaggaaaagc
    36601 cccatatcat caagtaatcc caacgcccac ttcgttttgc aactagaaat aagattagat
    36661 ggtagagtac atgaaagact aaaaatccaa taaaacctgg ataaagaagc ggtaccagac
    36721 tctctaattg acagatcatg atcacttcta ccaagactga aactaggata attccataat
    36781 acaggaaatt tgatttttga aaagagtttt tcatcattct cccttccttc acttctttcc
    36841 tgctatcctt tcttttattt tatcatatat ttatatagag tttaagccct aatattcttt
    36901 tgaaaaatac tttttttgct ttacttctat agtgaaaagg tttacaatta tagtaagaaa
    36961 atttcaggag gtttcattat ggcacaacgt taccaaaata tcatggtcgc aatcgatggt
    37021 tctaaggaag cggacttggc ttttgtcaag ggagttcatt ctgctctacg aaacgacgct
    37081 aaactcacca tcgcacatgt cattgacaca cgcgctctcc aaagcgtatc cacctttgat
    37141 gctgaagttt acgaagaact ccaagtcgac gctgaaagtc tgatgaaaga gtacgaaaaa
    37201 cgtgctaaag atgctggggt agcagatgtt catatcgtca ttgaaatggg aaatccaaag
    37261 accctgctag cacgtactat tccagatgcc gaggaagtgg acctcatcct cgttggcgca
    37321 actggtctca acgcctttga acgcctcttg gtcggctctt catctgaata tatactccgc
    37381 catgctaagg tcgatttgct ggttgtgaga gaacaagaaa aaaccttata atcacaaaga
    37441 aaaggagccc ctagctcctt tttgtttacg atttatttct ctctttatgg cgttcgtaag
    37501 ccttgagctg gcgctgcagt tcctttttaa tagcaggttc tggagcatat ttttcttccc
    37561 aattatctgg ttttaagatt ttatgggtca ctggatcaaa atgagccttg ccatctggaa
    37621 aaattttccc catattggcc tgatggacaa tatcaaaaat acgttctggg tccaccccca
    37681 tcaagacaaa actgccgtag gtgaagtaaa gcgtgtcaat caaggcatcc acttgcccta
    37741 tcaaatcttg ctgagcaggt gtcttcttgg ctactttatc tgctgcctta tcaagggtct
    37801 gatgaagttg cgatacagct tgaccaaaat cttcttcaga aggactggct gctcgaacaa
    37861 actccaccaa ttcttctatt ttaaaaccag ccctatgggt tgcaccctct aaatcccaag
    37921 ctcgaggttc ttcttgggtt cgttcatcca tcatgtggtg gaaggtcttg accttattga
    37981 aatgatagtc acggctgaca aagacttttt ctgaagacaa aacaccaaaa tgttccaagg
    38041 ccttgtggat tccatcttgt tgattgctag tggtaatgtg cttggcagct tccttgacac
    38101 tgctactacc atttcccata gcgaccgaca taccgacgcc agccagcatt tcaagatcat
    38161 tatctgagtc accaaaagcc atgacttggt tgaggtcaaa gccatattct ttcccaactc
    38221 ggcgaatgcc ttctaattta gaatttccct gattgatgac atccgatgca aaaggattgc
    38281 tacgtgtcaa tttcaagtct tcaaaatcag ctgccgcctt ctcagattct tctggtgtca
    38341 tcagcatcaa aacttggtag ataggctgat tcatcaggtg aagcaggtcc tcttcctttt
    38401 ggggaacaac cttgctgacc atgcgattaa aagactgact caccgtccga gttaaaacag
    38461 agggaacgaa gcgactaatt cgttgggaaa aagaacccag accaaaggac atgattttag
    38521 aacccaacat ggcatccttg gtccctagag caatctccgt gccctctttt ttagcatagc
    38581 taattagatg gcgcaaatgt aacttggaaa tagggctcgt gaacaagact ctgtctttac
    38641 taaagatata ctggccatta taggttaccg caaaatccag atccaaatcg tccatcaatt
    38701 ccttaacaaa aaaaggtcct cgccctgtcg ctacgccaac tagtacccct tgttctttga
    38761 caatcttaat cgcgtcctta gtggatttca aaacactctt gcgattgttg accaaggttc
    38821 catcgatatc aaaaaaaaca gctttgactt ccatcctatt ccaatctccc cttttgtgat
    38881 acaatgatta taccacattt cagaaagagt gagtaaatca tgcctaagaa aatccttgtt
    38941 ttacatacgg gtggaactat ttccatgcag gccgatgctt ctggcgctgt tgtgacgagt
    39001 tcagataatc ccatgaacca tgtgtccaac ccacttgaag gaatccaagt ccacgccttg
    39061 gactttttta accttccaag tccccatatc aaacccaaac atatgctggt cctctaccag
    39121 aaaattaaag aggaagcaga taactacgat ggagtggtga tcacacacgg aaccgatact
    39181 ttagaggaaa cagcctattt ccttgatacc atggaagttc cccatatgcc tatcgttcta
    39241 acaggagcca tgcgtagctc caatgagctc ggtagtgatg gtgtttataa ttacctaagt
    39301 gctttacgag tggccagcga tgacagggct gctgacaaag gagttttggt cgttatgaac
    39361 gatgaaatcc acgctgccaa gtatgtcacc aaaacacata cgactaatgt caacaccttc
    39421 cagactccaa cacatggccc cctcggtctc atcatgaaac aggaaatcct ctacttcaaa
    39481 acagctgaac ctcgtgttcg ctttgacctt gatcacatac aaggtttagt ccctatcatc
    39541 tcggcttatg ctggtatgac agatgagctg attgatatgc tggatttaga acacttggac
    39601 ggtttgatta tccaagcctt cggagctggt aatattccca aagaaacggc tcaaaaatta
    39661 gaaagccttc tgcaaaaagg aatcccagtc gctctggtat cacgatgctt taacggtatt
    39721 gccgagcctg tttatgccta ccaaggtggg ggcgtacagt tgcaaaaagc aggcgttttc
    39781 tttgttaaag aactcaacgc ccaaaaagcc cgcttgaaac tcctcatcgc cctcaatgcc
    39841 ggactaacag gacaggcttt gaaagactat atggaaggct aatactcttc gaaaatctct
    39901 gcaaaccacg tcacgtcgcc ttaccgtatg tatggttact gacttcgtca gttctatcta
    39961 caacctcaaa aacatgtttt gagc
//
LOCUS       DAVAAL010000005        38103 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVAAL010000005 DAVAAL010000000
VERSION     DAVAAL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.005"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            155..1600
                     /locus_tag="VQM60_000170"
     CDS             155..1600
                     /locus_tag="VQM60_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET1080082.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVYFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLMSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEHPDYASKIKKIYTKGFTKKVEAP
                     KTSKDQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSRIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGMGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDMLEGR"
     gene            1602..2333
                     /gene="cps4B"
                     /locus_tag="VQM60_000171"
     CDS             1602..2333
                     /gene="cps4B"
                     /locus_tag="VQM60_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HET1080083.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTSDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHIIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            2342..3034
                     /gene="cpsC"
                     /locus_tag="VQM60_000172"
     CDS             2342..3034
                     /gene="cpsC"
                     /locus_tag="VQM60_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET1080084.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPAMTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDVEDVLQMP
                     LLGVVPDFDKMK"
     gene            3044..3733
                     /locus_tag="VQM60_000173"
     CDS             3044..3733
                     /locus_tag="VQM60_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HET1080085.1"
                     /translation="MPTLEISQAKLELVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIVVDTPPVGVVI
                     DAAIIMQKCDASILVTKAGETKRRDLQKAKEQLEQTGKSCLGVVLNKLDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            3748..5109
                     /locus_tag="VQM60_000174"
     CDS             3748..5109
                     /locus_tag="VQM60_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET1080086.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNPFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRIIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGGSAFFAQTRIGKNGRHFTFYKFRSMCVDAEEKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            5115..5858
                     /locus_tag="VQM60_000175"
     CDS             5115..5858
                     /locus_tag="VQM60_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HET1080087.1"
                     /translation="MNERIQILGITIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCYEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMKHLLELSNEKG
                     YSVYFFGAKEDVLQDMLKIFEERYPALNVVGYRNGYFSPEDEKHIQEDIKEKKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQNAHLEWLFRVAN
                     EPKRLFKRYFVGNVSFIRKVLRAKRGYKL"
     gene            5855..6679
                     /locus_tag="VQM60_000176"
     CDS             5855..6679
                     /locus_tag="VQM60_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HET1080088.1"
                     /translation="MNQTEIVKLIQKVELDAIKEFQKICKENNIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREGYDKLPGIFKDRIIAGKYQVLAYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSFLRKLYFGKVYWYRFLASLGTTYVGDH
                     VDMHSAKQKLIIGFFKKLGFAKLFPQNYVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDAFFKVPTEYDRYLKRLYGENYLHEEPSDDEKKSHLGGQ"
     gene            6681..7559
                     /locus_tag="VQM60_000177"
     CDS             6681..7559
                     /locus_tag="VQM60_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET1080089.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNSNTKQIVIIDNFSNNGTG
                     EKLQELYESDSEIDVLINHENAGFARGNNVAYQFAKEKYKPDFMVIMNNDIEIETEEF
                     EKIVTDIYRKEKFHLLGPDIFSTTYQLHQNPKRLTHYTYEEVKALNEKFKKGSQVSLA
                     LKIKCWLKSSKVLRTAIYQNRRKKKSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIKVLHHQNVATNQVYTNLVEKTLFSNKCNF
                     ESTSYFLKLMKENEGV"
     gene            7560..8894
                     /locus_tag="VQM60_000178"
     CDS             7560..8894
                     /locus_tag="VQM60_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET1080090.1"
                     /translation="MTYLFLLCLTLFLLTFFYFFAFNQDLIAPPVVMSVMFLISSVFA
                     LINVQNWNIEYSGLAYLLIISGIIVFSMPLLALNSPSLNTKIKVTDRLIDIQFWKIAL
                     TIIVDLVILYLYRREIHNLALSHGYTGSNFQWFFRNATSYEGELTVRTSIRVLIRIID
                     VSAYIFGYTFINNFFIYSHKRSKDLLLLVPFLIFISKTLLSGGRLDIIKILIAYVVMA
                     YIQQKRKVGWDKVISHKYMRLGFVGLIAGIPTFYYSLFLSGRSTTRTVFESISTYLGG
                     SIQHFNQYIQNPIGVAEVFGDESFVAIMNILGNLGFVNYNSTVHLEFRQLGITMGNVY
                     TFFRRPWHDFGLVGMYIFSFVVGVFFAIFYLKLRKSRAGFKLDIHTIIYSYFFYWIFL
                     SSIEQYSFTTISLFTLVFIVLVYLMAFFYWNLDFHRGKLVIKLSDTSIKSEN"
     gene            8922..10346
                     /locus_tag="VQM60_000179"
     CDS             8922..10346
                     /locus_tag="VQM60_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080091.1"
                     /translation="MNSKIKNILTNFSYVISSNLLTVLTSSLVVLIFPKLMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYNNLDRKQFYSQMILFSSFLMIISLLLF
                     GLNVLAVTDSNARYIYNMTIISMIITNLRTLYVYVLQMTNRLKDSSIILLSDRVLYVL
                     LLFLFIVFGWHEYKVMILADILGRSFSLILSFWICKDIVFQPLSKFIFNIKESFDNIR
                     VGINLMLSNIASSLIIGIVRLGIQWNWNIETFGKVSLTLSVSNLLMTFINAIGLVVFP
                     LLRRTKAENLPKIYSNLRNVLMLIMFAILLIYYPLKIVLDLWLPAYQDALIFMTLIFP
                     MSVYEGKMALVINTYLKALRMERDILRVNALVMLISMGVTLVTTYLLNSLELTVVSIV
                     VLLALRSIIAELILSKKLDVSVKKDIVLEFLLTLVFISSSWYLPIGLAVIVYTIAYGL
                     YLYLKHEDIKTYLAYFKASKKTSN"
     gene            10411..11499
                     /gene="wecB"
                     /locus_tag="VQM60_000180"
     CDS             10411..11499
                     /gene="wecB"
                     /locus_tag="VQM60_000180"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET1080092.1"
                     /translation="MKKIMLVFGTRPEAIKMCPLVNELKKHEDMETIVCVTGQHKEMV
                     SPVLDLFGVVPDYDLEIMKANQTLFSITTSILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIATYHFAPTELAKENLLKE
                     GRENVYVTGNTVIDALTTTVQEDYTHTHLDLNANNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYEDVKVIYPIHKNPLVRETAAEIFGDTERIQIIEPLDVLDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKLLLDDSG
                     EYKKMSQASNPYGNGDASQQIVQILRGI"
     gene            11662..12531
                     /gene="rfbA"
                     /locus_tag="VQM60_000181"
     CDS             11662..12531
                     /gene="rfbA"
                     /locus_tag="VQM60_000181"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET1080093.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            12532..13125
                     /locus_tag="VQM60_000182"
     CDS             12532..13125
                     /locus_tag="VQM60_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET1080094.1"
                     /translation="MSDNFFGKTLVVRKIDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPALGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            13137..14186
                     /gene="rfbB"
                     /locus_tag="VQM60_000183"
     CDS             13137..14186
                     /gene="rfbB"
                     /locus_tag="VQM60_000183"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET1080095.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFTDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDLRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQDWWKAEKE
                     AVEANYAKTQEIITV"
     gene            14252..15103
                     /gene="rfbD"
                     /locus_tag="VQM60_000184"
     CDS             14252..15103
                     /gene="rfbD"
                     /locus_tag="VQM60_000184"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET1080096.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQSLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            15166..15500
                     /locus_tag="VQM60_000185"
                     /pseudo
     CDS             15166..15500
                     /locus_tag="VQM60_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272501.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
     gene            15767..16352
                     /locus_tag="VQM60_000186"
                     /pseudo
     CDS             15767..16352
                     /locus_tag="VQM60_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            16589..18571
                     /locus_tag="VQM60_000187"
     CDS             16589..18571
                     /locus_tag="VQM60_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET1080097.1"
                     /translation="MKSSRLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVVIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            18872..24175
                     /locus_tag="VQM60_000188"
     CDS             18872..24175
                     /locus_tag="VQM60_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET1080098.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGSVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPRAKVVITEDANADKKVDWQDGAIAYRSIMNNPKGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLPTDKEKMYYFNTQTGATTWTLPSDWAESKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FESLDAPAQPGEGLENAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTTTDWPDNNKSKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(24341..26500)
                     /locus_tag="VQM60_000189"
     CDS             complement(24341..26500)
                     /locus_tag="VQM60_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET1080099.1"
                     /translation="MNKQTILRIAKYVSICFLTLFITAVMLGGGIFLYYVSKAPALSE
                     SKLVATTSSKIFDSKNELIADLGSERRVNAQANEIPTDLVKAIVSIEDHRFFDHRGVD
                     TIRIIGAFLRNLQNNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAVQLEQK
                     ATKQEILTYYVNKVYMSNGNYGMQTAAQNYYGKDLRELSLPQLALLAGMPQAPNQYDP
                     YSHPEAALDRRNLVLSEMKGQGYISAEQYEKAINTPITDGLQSLKSVNSYPAYMDNYL
                     KEVIDQVEQETGYNLLTTGMDVYTNVDQEAQKHLWDIYNSDQYVSYPDDDLQVASTVV
                     DVSNGKVIAQLGARHQASNVSFGTNQAVETNRDWGSSMKPITDYAPALEYGVYDSTAS
                     IVHDVPYNYPGTDTPLYNWDHVYFGNITIQYALQQSRNVTAVETLNKVGLDRAKTFLN
                     GLGIDYPSMHYANAISSNTTESNKKYGASSEKMAAAYAAFANGGIYHKPMYINKIVFS
                     DGSEKEFSDAGTRAMKETTAYMMTEMMKTVLTYGTGRGAYLPWLPQAGKTGTSNYTDE
                     EIEKYIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIVGDGFLVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(26497..27093)
                     /gene="recU"
                     /locus_tag="VQM60_000190"
     CDS             complement(26497..27093)
                     /gene="recU"
                     /locus_tag="VQM60_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET1080100.1"
                     /translation="MVNYPHKLSSQKRQPSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVRVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEAKETKQ
                     KRAIPMKNFHPHQIQHMEQILAQQGICFVLLHFSSQQETYLLPAYDLIRFYHQDKGQK
                     SMPLGYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            27160..27687
                     /locus_tag="VQM60_000191"
     CDS             27160..27687
                     /locus_tag="VQM60_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET1080101.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGTLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            27757..28098
                     /gene="gpsB"
                     /locus_tag="VQM60_000192"
     CDS             27757..28098
                     /gene="gpsB"
                     /locus_tag="VQM60_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET1080102.1"
                     /translation="MTSIIFSAKDIFEQEFGREVRGYSKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPQVSSAPSPSHPDPIDVAASSSMTNFDILKRLNRLEKEV
                     FGKQILDNTDL"
     gene            28119..28499
                     /gene="rnpB"
                     /locus_tag="VQM60_000193"
     ncRNA           28119..28499
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VQM60_000193"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            28584..29741
                     /locus_tag="VQM60_000194"
     CDS             28584..29741
                     /locus_tag="VQM60_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET1080103.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWVSDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYSEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            29754..31148
                     /gene="mapZ"
                     /locus_tag="VQM60_000195"
     CDS             29754..31148
                     /gene="mapZ"
                     /locus_tag="VQM60_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET1080104.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLS
                     EDSILDKYVKQHRDEIEADKFATRQYKKEELVETQSLDDLIQEMREAVEESEAYSEEV
                     PSSEDILPPLPLDDEEQGLDPLLLEDENPTEMTEEVEEEQNLSRLEQEESEKKSKKGF
                     VLTALALVSVIICVSAYYVYRQVNRSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSETSSSSTS
                     QTSSNTTSETSPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            31224..32648
                     /gene="gndA"
                     /locus_tag="VQM60_000196"
     CDS             31224..32648
                     /gene="gndA"
                     /locus_tag="VQM60_000196"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET1080105.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEVS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            32660..33349
                     /locus_tag="VQM60_000197"
     CDS             32660..33349
                     /locus_tag="VQM60_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1080106.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            33447..34409
                     /gene="cbpC"
                     /locus_tag="VQM60_000198"
     CDS             33447..34409
                     /gene="cbpC"
                     /locus_tag="VQM60_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET1080107.1"
                     /translation="MKILKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDGGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYVVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWYQDGSTWYYLDPSN
                     GDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            34428..35426
                     /gene="cbpJ"
                     /locus_tag="VQM60_000199"
     CDS             34428..35426
                     /gene="cbpJ"
                     /locus_tag="VQM60_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HET1080108.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWLVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNNWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQYLGNKWYYLRSSGAMVTGW
                     YQDGLTWYYLNAGNGDMKTGWFQVNGKWYYAYSSGALAVNTTVDGYYVNYNGEWVR"
     gene            complement(35544..36776)
                     /locus_tag="VQM60_000200"
     CDS             complement(35544..36776)
                     /locus_tag="VQM60_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET1080109.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLAMLSIIIS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(36935..37405)
                     /locus_tag="VQM60_000201"
     CDS             complement(36935..37405)
                     /locus_tag="VQM60_000201"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080110.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKSNR"
     gene            complement(37305..37663)
                     /locus_tag="VQM60_000202"
                     /pseudo
     CDS             complement(37305..37663)
                     /locus_tag="VQM60_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
BASE COUNT        11966 a         6575 c         8326 g        11236 t
ORIGIN      
        1 gatcgatttg tcatgttctt atttcatttt actatatttt tgtttcgcgg gaagtctact
       61 aagatactta aagatgcaga tagtaaaaaa aaatgtagac attaccgtaa aaaagtgata
      121 taatcgtacg gtgttcaagg tataggtgtt aatcatgagt agacgtttta aaaaatcacg
      181 ttcacagaaa gtgaagcgaa gtgttaatat cgttttgctg actatttatt tattgttagt
      241 ttatttttta ttgttcttaa tctttaagta caatatcctt gcttttagat atcttaacct
      301 agtggtaact gcgttagtcc tactagttgc cttggtaggg ctactcttga ttatctataa
      361 aaaagctgaa aagtttacta tttttctgtt ggtgttctct atccttgtca gctctgtgtc
      421 gctctttgca gtacagcagt ttgtgggact gaccaatcgt ttaaatgcga cttctaatta
      481 ctcagaatat tcgatcagtg ttgctgtttt agcagatagt gagattgaga atgttacgca
      541 actgatgagt gtgacagcac cgactgggac tgataatgaa aacattcaaa aactactagc
      601 tgatatcaaa tcaagtcaga ataccgattt gacggtcaat cagagttcgt cttacttggc
      661 agcttataag agtttgattg caggagagac taaggccatt gtcttaaata gtgtctttga
      721 aaatatcatc gaatcggagc atccagatta cgcatcgaag ataaaaaaga tttataccaa
      781 gggattcact aaaaaagtag aagctcctaa gacgtctaag gatcagtctt tcaatatcta
      841 tgttagtgga attgatactt atggtccgat tagttcggtg tcgcgttcag atgtcaatat
      901 cctgatgact gtcaatcgag ataccaagaa aattctcttg accacaacgc cacgtgatgc
      961 ttatgtacca atcgcagatg gtgggaataa tcaaaaagat aaattgaccc atgcgggcat
     1021 ctatggagtt gattcgtcca tccacacttt agaaaatctc tatggagtgg atatcaatta
     1081 ctatgtgcga ttgaacttca cttctttctt gaaattgatc gatttgttgg gtggggtaga
     1141 tgtttataat gaccaagatt ttacatctct acacgggaag ttccatttcc cagttggaaa
     1201 tgtccactta gattctgagc aggctctggg atttgttcgt gagcgctatt ccctagcgga
     1261 tggtgatcat gaccgcgggc gcaatcaaca aaaggtgatt gcggccatcc ttcaaaaatt
     1321 aacttcgagc gaagcgctga aaaattatag taggatcatt gatagcttgc aagattctat
     1381 ccaaacaaac atgccacttg agaccatgat aaacttggtc aatgctcagt tagaaagtgg
     1441 tggaacgtac aaagtgaatt cgcaagactt gaagggtatg ggacggacgg atcttccttc
     1501 ctatgcgatg ccagatagta acctctatat gatggaaatt aacgacagta gccttgcatc
     1561 tgtcaaaact gctattcagg atatgttgga gggcagatga aatgattgat attcattcgc
     1621 acatcgtctt tgatgtagat gatggtccca agtcaagaga ggaaagcaag gctctcttgg
     1681 cagaatccta caggcagggg gtgcgaacca ttgtctctac ctctcaccgt cgcaagggca
     1741 tgtttgaaac tccggaagag aagatagcag aaaactttct tcaggttcgg gaaatagcta
     1801 aggaagtggc gagtgacttg gtcattgctt acggggctga aatttactac acatcagatg
     1861 ttttggataa gctggaaaaa aatcggattc cgaccctcaa taatagtcgt tatgccttga
     1921 tagagtttag tatgaacacc ccttatcgcg atattcatag tgccttgagt aaaatattga
     1981 tgttgggaat tactccagtc attgcccaca ttgagcgcta tgatgcactt gaaaataatg
     2041 aaaaacgcgt tcgagagctg atcaatatgg gctgttacac gcaagtaaat agttcacatg
     2101 tcctcaaatc caaacttttt ggagaacgtt ataaattcat gaaaaaaaga gctcagtatt
     2161 ttttggagcg tgacttggtt catatcattg caagtgatat gcataatgtg gacagcagac
     2221 ctccccatat ggcagaagca tatgaccttg tttcccaaaa atacggagaa gcgaaggctc
     2281 aggaactttt tatagataat cctcgaaaaa ttgtaatgga tcaactaatt taggagaaat
     2341 gatgaaagaa caaaacacga tagaaatcga tgtatttcaa ttacttaaaa ccttgtggaa
     2401 acacaagcta ataattttat tagtggcact tgtgacaggg gcgggagctt ttgcatatag
     2461 cacttttatt gttaagccag aatatacaag caccacgcgt atttacgtag tcaaccgtaa
     2521 tcaaggagat aagccgggac tgacgaatca ggacttgcag gcaggaactt atctggtaaa
     2581 agactaccgc gaaattatcc tttcgcaaga tgtattggaa aaggtagcga caaatttgaa
     2641 attggatatt ccagcaaaaa cgttaactag caaagtgcaa gtgactgtac cagccgacac
     2701 tcgtatcgtc tcaatctctg tcaaggataa acaaccagag gaagccagtc gcattgctaa
     2761 ttctctacga gaagttgctg cagaaaagat catcgctgta acgcgagtat ctgatgtaac
     2821 gacgcttgaa gaagcgcgac cagctatgac tccctcttct ccaaatgttc gacgcaacac
     2881 cttagttggt tttcttggag gagccgccgt aacagtaatt actgttcttt tgattgagtt
     2941 gctcgatacc cgtgtaaaac gtcctgaaga tgttgaagat gtactgcaaa tgccacttct
     3001 aggagtcgtt ccagattttg ataaaatgaa ataggaggaa gttatgccaa cgttagaaat
     3061 ctcacaggcg aaattagaac ttgtgaaaaa ggcagaggaa tattataatg ctttgtgcac
     3121 gaacctacag ttaagtggag atgatttgaa agtattttct atcacttctg tgaaagcagg
     3181 agaaggaaaa acaacgactt ccaccaatat cgcttgggct tttgcgcgtg caggttacaa
     3241 aacgcttctg attgatgcag acatgcgtaa ctccgttatg tcaggtgtct ttaaatcaag
     3301 ggaaaggatt actggactaa cagaatttct atcagggaca acagacctgt cacaggggct
     3361 ttgcgatacc aatattgaga atctctttgt gattcaggct ggctctgtgt caccaaatcc
     3421 gacagccctt cttcaaagta agaatttcag tacaatgctt gaaaccttgc gtaaatattt
     3481 tgactatatc gtcgtagaca ctccccctgt cggtgtcgtg attgatgcgg ctatcattat
     3541 gcagaaatgt gatgcttcta ttttagtgac gaaggcaggt gaaacaaaac gacgggatct
     3601 tcaaaaagcg aaagaacagt tggaacaaac tgggaagtcg tgtttaggag ttgtgttgaa
     3661 taaactcgat acttcagtag agaaatacgg ttcttatggg agttatggga attacaggaa
     3721 acagaaaaaa taggttgagg gatagaaatg agtgaaaaac tagcaaagcc ttcagtagcc
     3781 atagtccaga gttttcttgt tattttattg acttatctac ttagcgctgt gagagaaaca
     3841 gagattgttt caacaacagc tattgtactt tatatcctcc actattttgt cttttatatc
     3901 agtgattatg gacggaattt ctttaaaagg ggatatttga ttgaacttgt ccagacattg
     3961 aaatatatcc tattctttgc gctagcgatt agtatttcta attttttctt agaagatcga
     4021 tttagtattt ccagaagagg catgatttac ttcctcttat tacatgtttt cttagtctat
     4081 gtgctaaacc catttatcaa gtggtattgg aagcgggctt atcccaactt taaaggaagt
     4141 aagaagattc tcctacttac agcaacttct cgtgttgaaa aggtactgga tagattaata
     4201 gaatcaaatg atgttgttgg ggagttggta gccgtcagtg ttttagataa accagatttt
     4261 cagcatgatg atttaaaggt tgtagcagag ggggagatag taaattttgc gactcatgag
     4321 gtggtcgatg aagtctttat caatcttcca agtgaaaaat acaatattgg agagcttgtc
     4381 tctcagtttg aaacgatggg aattgatgta acagtcaatc taaatgcttt cgattgggca
     4441 cgtaacaagc aaatttgtga gatggcagga ctaaatgttg tgactttttc tacaacattt
     4501 tataagacta gccatgtgat tgctaagcgg attattgata ttgtcggttc cctggtaggt
     4561 ttgatattat gtggtctagt cagtattgtg ctggttcctt tgattcgaaa ggatgggggt
     4621 tctgcttttt ttgctcagac gcgtatagga aaaaatggcc gccattttac tttttataag
     4681 tttcgctcta tgtgtgtgga tgctgaggag aaaaaaagag aactcatgga acaaaatacc
     4741 atgcagggtg gaatgtttaa ggtggacgat gatcctcgta tcacgaaaat tggtcatttt
     4801 atacggaaga cgagcttgga cgagctacca cagttttata atgttctaaa gggagatatg
     4861 agtttggtag gtacacgacc accaacagtg gacgagtatg agcactatac cccagaacaa
     4921 aaacgtcgcc taagttttaa acctggcata acaggtttat ggcaggtcag cggacgaagt
     4981 gaaatcaaaa atttcgatga agttgtcaaa ttagatgtgg cctatattga tggttggaca
     5041 atctggaaag atattgaaat tttattgaag acagttaagg ttgtattgat gaaggatgga
     5101 gcgaaatagt tcatatgaat gagagaattc aaatattagg aattactata gatccattaa
     5161 cgatgaaaga aacagtggat gctgttgaac agtatgtttt aaagaagcac cctttgcact
     5221 tgatgggagt taacgcggat aaaattaatc agtgctatga agatgagaaa atcaaaaaaa
     5281 tcgttaatga atcaggaata attaatgccg acggtgcttc agtagtttta gcgagtaagt
     5341 ttttaggaac accagttcct gagcgagttg ctggaataga cttgatgaaa catttactag
     5401 agttgtctaa tgaaaaagga tactcggtct acttttttgg agcaaaagaa gacgttttac
     5461 aagatatgtt aaaaattttt gaagaaagat atccagctct aaatgttgtt ggctatagaa
     5521 atggatattt ttcgcctgaa gatgaaaaac atattcagga agatattaaa gagaaaaaac
     5581 cagattttgt gtttgtagga attacttctc ctaagaaaga atatattatc caatcattca
     5641 tggataatgg aattaatgct gtgtttatgg gggttggtgg aagttttgat gtactatcag
     5701 ggcatatcaa acgagctcca ttatggatgc aaaatgctca cctagagtgg ttgtttcgtg
     5761 tggcaaatga gcctaaacgt ctctttaagc gttattttgt agggaatgtt tcatttataa
     5821 gaaaagtttt aagagcgaaa aggggatata aactttgaac caaacagaaa tagttaaatt
     5881 gattcagaaa gttgaattgg atgctataaa agagtttcaa aaaatttgta aagagaacaa
     5941 tatagatttc ttccttcgtg gggggagtgt acttggggca gtcaaatatg atggctttat
     6001 tccatgggat gatgacatgg atattgctgt gcctcgtgaa ggctacgata aacttccagg
     6061 tatctttaag gatagaatta tcgcaggcaa atatcaggtt cttgcttatc aatattgcga
     6121 tactttacat tgctactttc ctcgcttatt ccttttagaa gatgaaagaa aacgtttggg
     6181 attgccacga aataccaatt taggtttgca tttgattgat atcattcctt tggatggtgc
     6241 cccaaatcat tcatttttaa gaaagcttta cttcggtaaa gtatactggt atcgtttttt
     6301 agcaagttta ggaactacct atgttggaga ccatgtggat atgcattccg ctaagcaaaa
     6361 actaatcatt ggtttcttta aaaagctagg atttgcaaaa ctattccctc aaaattatgt
     6421 gtacagacgc ttggataatc tctataaaaa gtatgattgg aaaaagcaga agtatgctgg
     6481 gacaattaat gcatctctgt ttgccaaaga agtcatgcca gtagagattt ggggagaagg
     6541 agtagagaag ccttttgagg atgccttctt taaagttccc acggagtatg accgctatct
     6601 gaaaagactt tacggagaaa actatcttca cgaagagcct agtgatgatg aaaagaaatc
     6661 gcatttagga ggacaataat ttgttttgtt atattatttt gcattacaaa gtcttagagg
     6721 aaactatttc ttgtgttaaa tcaataaaag aaggcaattc caatacaaag cagatcgtta
     6781 ttattgataa tttttctaat aacggtacgg gtgaaaaact acaagagctg tatgagtcag
     6841 attcagagat tgatgtcttg attaaccatg aaaatgctgg tttcgctcga ggtaataatg
     6901 tagcatatca gtttgctaag gaaaagtaca aacctgattt tatggttatc atgaataatg
     6961 atattgagat agaaacagaa gagtttgaaa aaatcgtgac agatatctat cggaaggaaa
     7021 aattccattt gttaggacca gatatctttt cgacgacgta tcagcttcac caaaacccaa
     7081 aacggttgac gcattatact tatgaagagg ttaaggctct caatgaaaaa tttaagaaag
     7141 ggagccaagt tagtctagca ttaaaaatta aatgttggtt gaagtctagt aaagttcttc
     7201 ggacagcaat ctatcaaaat aggcgtaaaa agaaatcagt agactataga aaacaggtag
     7261 aaaacccaat tcttcatggt tcgtttattg tatattctag agattttatt gagaaagagg
     7321 agtatgcttt taatcccaat accttcttct attatgaaac agagatatta gattatgaag
     7381 ctgagttaaa aggatataag agaatttata caccgaagat taaggtcttg caccatcaaa
     7441 atgttgcaac caatcaagtc tatacaaact tagtagaaaa aaccttgttt tcaaacaaat
     7501 gcaactttga atccactagt tattttttga agttgatgaa agaaaacgag ggtgtgtaaa
     7561 tgacttattt atttttactc tgcctgacct tattcttatt aactttcttc tatttttttg
     7621 ctttcaatca agatttgata gctccgccag ttgtcatgtc tgttatgttt ctcattagtt
     7681 ctgtatttgc ccttattaat gtgcaaaact ggaatattga gtatagtggt ttagcttatc
     7741 ttttgattat tagtggcatt attgtttttt caatgcctct cttggctctt aattcgccta
     7801 gtctaaatac caagattaaa gtgacggatc ggctcattga tatccaattc tggaaaatag
     7861 ctcttactat tatagttgac ctcgttattc tatatcttta taggagagag attcataatc
     7921 ttgcacttag ccatggttat acgggttcaa attttcagtg gttctttaga aatgctacca
     7981 gttatgaagg tgagctaaca gtgcgaactt cgattcgggt cctcattcgt atcattgacg
     8041 tatctgctta tatttttgga tatactttta ttaataattt cttcatttat agtcataaac
     8101 gctctaaaga tttactgctc ttagttccat tcttgatttt tatttctaaa accttattat
     8161 ctgggggtag attggatatt ataaaaattt taattgcgta tgttgtaatg gcctatattc
     8221 agcaaaaacg aaaagttggc tgggataagg tcatctccca taaatatatg agacttggtt
     8281 ttgtaggctt gatagctggg attcctacct tttactattc tttattctta tctggacgtt
     8341 ctacgactag aactgtattt gaaagtattt caacctattt aggaggttcg attcagcatt
     8401 ttaatcagta tattcaaaat cctattggag tagctgaggt ttttggcgat gagtcatttg
     8461 tagctattat gaatattttg ggtaatcttg gctttgtcaa ttacaatagt accgttcact
     8521 tagaatttcg gcagttaggg attactatgg gtaacgttta tactttcttt agaagaccct
     8581 ggcatgactt tgggttagtc ggtatgtata tcttttcctt tgttgtcggt gtgttttttg
     8641 ctatttttta tttgaaatta agaaagagcc gtgctggttt taaattggat attcatacaa
     8701 ttatttactc ttatttcttt tattggattt ttttatcgtc cattgagcaa tactcgttta
     8761 caacaatcag tttgtttaca cttgtattta tagtcttggt ttacctaatg gctttcttct
     8821 attggaattt ggatttccac agaggaaaac tagtaattaa actctctgac acaagtatta
     8881 aatcggaaaa ctgaaaacaa taaaaataga gaggatagtt gatgaatagc aaaattaaaa
     8941 atatactaac taatttctct tatgtcattt cttcaaatct tttgacagtc ttgacctctt
     9001 ccttggttgt cttaattttc cccaaattaa tgggggtaac agagtacagt tactggcaac
     9061 tttatatttt ttatctgacc tatatcggtt ttttccactt gggttggatt gatgggattt
     9121 atcttaaata tggtggctta gaatataata atttagatag aaaacagttt tattctcaga
     9181 tgattctatt ttctagcttc ttaatgatta tctccttgct attgtttggt ttaaatgtat
     9241 tagctgtaac agattcgaat gcacgatata tttataatat gactattata agtatgataa
     9301 tcacaaattt aagaacgctc tacgtgtatg ttttgcagat gacaaaccgc ttgaaggata
     9361 gttccatcat tctactcagt gatcgcgttt tatatgtgct ccttttattc ctgtttattg
     9421 tatttggttg gcatgaatac aaggttatga ttttggcaga tattctaggt cgatcatttt
     9481 ctctcatact ttccttctgg atttgtaaag atattgtgtt tcagcctttg tcaaaattca
     9541 tctttaatat aaaagagtct tttgataata tccgagttgg tatcaattta atgttatcca
     9601 acatcgcaag tagtttgatt atcggtattg ttcgtctagg aatccaatgg aactggaata
     9661 tcgaaacatt cgggaaagta tctctgacct tgagtgtttc caatctattg atgactttca
     9721 ttaatgccat tggactagtc gtctttccgc tattaagacg aacaaaagca gaaaacttac
     9781 ctaagattta ttctaattta agaaacgttt tgatgcttat catgttcgcg attttgctca
     9841 tttactatcc tttaaaaatt gtattagacc tctggttgcc agcctatcaa gatgccttga
     9901 ttttcatgac ccttattttc cctatgtcag tctatgaagg gaaaatggca ttggtcatta
     9961 atacttactt aaaggcatta agaatggaaa gagatatcct tagagtcaat gccttagtaa
    10021 tgttgatcag tatgggagtg accctggtta caacatacct gttaaatagt ttggagctga
    10081 ctgttgtatc gatagttgtt ttgctagctt tgagaagtat catagccgaa ttaattctgt
    10141 ctaaaaaact ggatgtttcg gttaagaagg atattgtatt agaatttctt ttgacgcttg
    10201 tctttatttc ttcaagttgg tatttgccga ttgggctggc agtaattgtc tacacaatag
    10261 cctacggttt atatctctat ttgaaacacg aagatatcaa aacctattta gcttacttta
    10321 aagctagtaa aaaaacatca aattaaaaaa ttatataatg attaagtggt agattctatt
    10381 tctaccgttt tagatattcg ggaggtaatg atgaaaaaaa ttatgttagt attcggtaca
    10441 cgtccggaag ccatcaaaat gtgtccatta gtcaatgagt tgaaaaaaca cgaagatatg
    10501 gaaacaattg tgtgtgttac tggacaacac aaagagatgg ttagtcctgt tttagattta
    10561 tttggtgttg taccagatta tgatttagaa attatgaagg ctaaccaaac cttgttctct
    10621 atcacaacta gtatcttgga aaagataaaa ccagttttag agaaggaaca accagatatt
    10681 gtcctagttc acggtgacac tacgacaact tatgcagcag ccttggcagc attctatttg
    10741 ggaattaaag taggacatgt tgaagctggt ttgcgaacgt acaatttaca aagtccattt
    10801 cctgaagaat ttaacaggca atcgacatca atcattgcaa cttaccattt tgctccaact
    10861 gagttggcta aagaaaatct cttaaaagaa ggtagagaga atgtttatgt gactggaaat
    10921 actgtcattg atgctcttac aactactgtt caagaggatt atacacacac tcatttagat
    10981 ttaaacgcta acaatcgtct catcttattg actgctcata gacgcgaaaa tctcggcgaa
    11041 ccgatgagac atatgtttag agcagttaaa cgagtattga atgaatacga agatgttaaa
    11101 gtcatttatc caattcataa gaatcctttg gtacgtgaaa cagctgcaga gatctttgga
    11161 gatacagaac ggattcaaat tattgaacct ttggatgttc ttgatttcca caacttcatg
    11221 aaccaaagtt atatgatttt aacagattct ggcggagttc aggaagaagc accttcttta
    11281 ggaaaacctg tattggttat gcgcgatact actgaaagac ctgaaggagt agcggcagga
    11341 accttgaaat tagtaggaac tgacgaagaa acaatttatc aaaacttcaa actacttcta
    11401 gatgattctg gagaatacaa aaaaatgagt caggctagca atccatacgg taatggtgat
    11461 gctagtcaac agattgttca gattttgcgt gggatttaag aagattctct aaaagagtct
    11521 aagaggaatc cactccacaa acataaaact cttatgctat catttatgat tgagatagac
    11581 tcataactta atatttttat cctaagaaaa ttcgttcata ttcaatatga atgaaaaaca
    11641 acgaagaaag gttatttcat tatgaaaggt attattcttg caggcggctc aggtacccgc
    11701 ctgtacccac ttactcgggc tgcgtcaaaa cagctgatgc cggtttatga taaacctatg
    11761 atttattatc cgttgtcgac attaatgttg gctggaatta aagatatttt gattatctca
    11821 actcctcaag atttgccccg ttttaaggac ttgctcttgg atggttccga atttgggatc
    11881 aagctttcct atgcggaaca acctagtccc gatggacttg ctcaggcttt tcttatcggt
    11941 gaagaattta tcggtgacga tagcgttgcc ttgattttag gcgacaatat ctaccatggt
    12001 cctggtctga gcacaatgct tcaaaaagca gccaagaaag agaaaggtgc gactgttttt
    12061 ggctaccaag tgaaggatcc agagcgtttt ggtgtggttg agtttgatac agacatgaat
    12121 gctatttcta tagaagaaaa accggagtat cctcgctcca actatgcagt gacaggactg
    12181 tatttctatg ataatgatgt tgtggagatt gctaaacaga tcaaacctag tgctcgtggc
    12241 gagttagaaa ttacagacgt taacaaggct tacctaaatc gtggtgacct ttctgttgag
    12301 ctgatggggc gtggttttgc ctggttggat acgggaaccc atgaaagctt gctagaagct
    12361 tctcagtata ttgaaacggt tcagcgtatg cagaacgttc aagtggcaaa tctagaagaa
    12421 atttcctatc gtatgggcta cattagtcgt gaagacgtgc tggaattggc tcagcctctt
    12481 aagaaaaatg aatacggaca atatttgctc cgtttgattg gagaagcata gatgtcagat
    12541 aatttttttg gaaagacact tgtggtacgc aagattgatg ctataccagg actgctagag
    12601 tttgatattc ccgttcatgg agacaatcgt ggttggttta aggaaaattt ccagaaggaa
    12661 aagatgctac cgcttggttt tcctgaaagc ttctttgctg cagggaaact gcaaaataac
    12721 gtcagctttt ctcgcaaaaa tgttcttcga ggactccatg ctgaaccttg ggacaagtat
    12781 atctctgttg cagacgatgg gaaggtttta ggatcttggg tagatttgcg tgaaggcgag
    12841 acctttggaa atgtttacca gacagagatt gatgctagca agggaatttt tgtccctcgt
    12901 ggcgtagcca acggttttca agtcctctca gatacggttt cctatagcta tctggtcaat
    12961 gactactggg ctcttgaact caaacccaaa tatgcctttg ttaactatgc tgacccagca
    13021 ttgggaattg agtgggaaaa cctagcggaa gctgaggttt cagaggcaga caaacaccat
    13081 cctctattaa gggatgtcaa accacttacg aaagacgagt tgtaaaagga aacattatga
    13141 ctgaatacaa aaaaattatc gtgacaggtg gagctggttt tatcggttct aactttgttc
    13201 actatgttta caataacttt acagatgtcc atgtgacagt gctggacaag ctgacttatg
    13261 caggtaatcg tgccaatatt gaagaaattt taggcgaccg tgttgagtta gtcgttggag
    13321 atattgctga tgcagccttg gtagataagt tagcagctga agcggatgcc attgtacact
    13381 atgcggcaga aagccacaat gacaattcgc ttaatgaccc ctcgccattt atccatacca
    13441 acttcatcgg aacttataca ctcttggaag cggctcgtaa atacgacctt cgtttccacc
    13501 atgtgtcgac tgacgaagtc tatggggatc tgccgctgcg tgaagattta ccaggtcatg
    13561 gggaaggacc aggtgagaaa tttacggctg aaaccaagta caatccaagc tcgccttact
    13621 catcgactaa ggcggcttca gacttgattg tcaaagcttg ggtgcgctca tttggtgtta
    13681 aagcaacgat ttctaactgt tcaaacaact atggtcctta ccagcacatc gagaagttca
    13741 ttccgcgcca gattactaat atcttgagcg gtatcaagcc aaaactctac ggagaaggta
    13801 agaatgtgcg tgactggatt catactaatg accattcatc aggtgtttgg acgattctga
    13861 ccaagggtca aatcggtgaa acctacctga ttggtgcgga tggtgagaag aacaataagg
    13921 aagtgttgga actcatcctc aaagaaatgg gacagccagc tgatgcttat gaccacgtaa
    13981 cagatcgagc tggtcacgac ctccgctatg ccatcgatgc aagcaagctc cgtgatgaac
    14041 taggatggaa gccagagttt accaactttg aagcaggtct caaagcgact atcaagtggt
    14101 acacagataa ccaagactgg tggaaagcag aaaaagaagc tgttgaagcc aattatgcta
    14161 agactcagga gattattaca gtataaaaag caggaaatcg ctgcttttta ttgctatatt
    14221 gggaagagtt acatattaga aaggtctaga gatgatttta attacagggg caaatggcca
    14281 attaggaacg gaacttcgct atttattgga tgaacgtaat gaagaatacg tggcagtaga
    14341 tgtggctgag atggacatta ccaatgaaga aatggttgag aaagtttttg aagaggtgaa
    14401 accaacttta gtctaccact gtgcagccta tactgctgtt gatgcagcag aggatgaagg
    14461 aaaagagttg gacttcgcca tcaatgtgac ggggacagaa aatgtcgcca aagcatctga
    14521 aaagcatggt gcaactctag tttatatttc tacggactat gtctttgacg gtaagaaacc
    14581 agttggacaa gagtgggaag ttgatgaccg accagatcca cagacagaat atggacgcac
    14641 taagcgtatg ggggaagagt tagttgagaa gcatgtttct aatttctata ttatccgtac
    14701 tgcctgggta tttggaaatt atggcaaaaa cttcgttttt accatgcaaa gtcttgcgaa
    14761 aactcataag actttaacag ttgtaaatga ccagtacggt cgtccgactt ggactcgtac
    14821 cttggctgag ttcatgacct acctagctga aaatcgtaag gaatttggtt attatcattt
    14881 gtcaaatgat gcgacagaag acacaacatg gtatgatttt gcagttgaaa ttttgaaaga
    14941 tacagatgtc gaagtcaagc cagtagattc cagtcaattt ccagccaaag ctaaacgtcc
    15001 gctaaactca acgatgagcc tggccaaagc caaagctact ggatttgtta ttccaacttg
    15061 gcaagatgca ttgcaagaat tttacaaaca agaagtgaga taagtagtag aatgattttc
    15121 tagtctaata aaagaggcag ataatgaact caaaaggagc ttaaggtgta cgattatctt
    15181 gttgttggtg ctggtctctt tggtgcagtc tttgcccatg aagtagcctt aaaaggaaaa
    15241 aagtaaaagt cattgaaaaa cggaatcata tcgtgggtaa tatctatact cgtgaagagg
    15301 aaggaattca agttcatcaa tatggtgctc atatcttcca tacttctgat aaggagagct
    15361 gggattatgt aaatcagttt gcagggttta accgttacac caattctcct gttgcaaact
    15421 ataagggtga gatttataac cttcctttta atatgaatac tttcaataaa ctctggggag
    15481 ttgtaacgcc agcagaagca caagctaaga ttgaggaaca acgtgctatt ttaaatggta
    15541 aaactcctga aaatttggaa gaacaggcga tttctcttgt aggtacagac atctacgaaa
    15601 aattaatcaa agactataca gagaaacagt ggggcaaacc aactactgaa cttccatcct
    15661 ttattattcg ccgtttacca gtacacctga cctatgataa caactatttt aacgatacct
    15721 atcaagggat tccaattggt ggatacactc aaatagttga aaaatgttgg attatgaaaa
    15781 tattgatgta gaaacaaatg ttgatttctt tgtgaacaaa gagcaatatc tgaaagattt
    15841 tcctaagatt gtctttactg gtatgattga tgaattcttt gactataagt tgggcgaact
    15901 agagtaccgt agtcttcgtt ttgaaaatga gaccttggat atggaaaatt accaaggaaa
    15961 tgcagttgtg aactatacgg atgcagaaac cccatatact cgcattattg aacacaaaca
    16021 ttttgagttt gggagtcaag caaagactat cattactaat agaacattct aaaacatggg
    16081 aaaaaggtga tgagccttat tatccagtta ataatgatcg taataatcat ttgtataaat
    16141 catataaaaa acttgctgat gagcaaggga atgttatctt tggtggccgc ttaggacact
    16201 atcgttatta cgatatgcac caagtaattg gagcagcttt gcagtgcgtg agaaatgagt
    16261 tagattcata ctcaatgaaa attaaagagc aaactaggaa gctagccgca ggttgctcaa
    16321 aacactgttt tgaggttgca gatggaagct gacgcggttt gaagagattt tcgaagagta
    16381 taaacaagta aaactgacta ccagttatta tttagaaata gtattaaaaa ttccttgact
    16441 atatgatata gttgagggat ttttaaatga tattcatatt ttttgcaaag atgttgtttg
    16501 aaaaataatt ttcaaaaatt ctgaaaattc tgttgacaac tttctgaaaa gagtctataa
    16561 tggagagaaa gttttaaagg agaaaatgat gaaaagttca agactatttg cccttgcggg
    16621 cgtgacatta ttggcggcga ctactttagc tgcatgctct ggatcaggtt caagcactaa
    16681 aggtgagaag acattctcat acatttatga gacagaccct gataacctca actatttgac
    16741 aactgctaag gctgcgacag caaatattac cagtaacgtg gttgatggtt tgctagaaaa
    16801 tgatcgctac gggaactttg tgccgtctat ggctgaggat tggtctgtat ccaaggatgg
    16861 attgacttac acttatacta tccgtaagga tgcaaaatgg tatacttctg aaggtgaaga
    16921 atacgcggca gtcaaagctc aagactttgt aacaggacta aaatatgctg ctgataaaaa
    16981 atcagatgct ctttaccttg ttcaagaatc aatcaaaggg ttggatgcct atgtaaaagg
    17041 ggaaatcaaa gatttctcac aagtaggaat taaggctctg gatgaacaga cagttcagta
    17101 cactttgaac aaaccagaaa gcttctggaa ttctaagaca accatgggtg tgcttgcgcc
    17161 agttaatgaa gagtttttga attcaaaagg ggatgatttt gccaaagcta cggatccaag
    17221 tagtctcttg tataacggtc cttatttgtt gaaatccatt gtgaccaaat cttctgttga
    17281 atttgcgaaa aatccgaact actgggataa ggacaatgtg catattgaca aagttaaatt
    17341 gtcattctgg gatggtcaag ataccagcaa acctgcagaa aactttaaag atggtagcct
    17401 tacagcagct cgtctctatc caacaagtgc aagtttcgca gagcttgaga agagtatgaa
    17461 ggacaatatt gtctatactc aacaagactc tattacgtat ctagttggta caaatattga
    17521 ccgtcagtcc tataaataca catctaagac cagcgacgaa caaaaggcat cgactaaaaa
    17581 ggctctctta aacaaggatt tccgtcaggc tattgccttt ggttttgatc gtacagccta
    17641 tgcctctcag ttgaatggac aaactggagc aagcaaaatc ttacgtaata tctttgttcc
    17701 accaacattt gttcaagcag atggtaaaaa ctttggcgat atggtcaaag agaaattggt
    17761 cacttatggg gatgaatgga aggatgttaa tcttgcagat tctcaggatg gtctttacaa
    17821 tccagaaaaa gccaaggctg aatttgctaa agctaaatca gctttacaag cagaaggtgt
    17881 gactttccca attcatttgg atatgccagt tgaccagaca gcaactacaa aagttcagcg
    17941 cgtccaatct atgaaacaat ccttggaagc aactttagga gcggataatg tagtcattga
    18001 tattcaacaa ctacaaaaag acgaagtaaa caatattaca tattttgctg aaaatgctgc
    18061 tggtgaagac tgggatttat cagataatgt cggttggggt ccagactttg ccgatccatc
    18121 aacctacctt gatatcatca aaccatctgt aggagaaagt actaaaacat atttagggtt
    18181 tgactcaggg gaagataatg tagctgctaa aaaagtaggt ctatatgact acgaaaaatt
    18241 ggttactgag gctggtgatg agactacaga tgttgctaaa cgctatgata aatacgctgc
    18301 agcccaagct tggttgacag atagtgcttt gattattcca actacatctc gtacagggcg
    18361 tccaatcttg tctaagatgg taccatttac aataccattt gcattgtcag gaaataaagg
    18421 cacaagtgaa ccagtcttgt ataaatactt ggaacttcaa gacaaggcag tcactgtaga
    18481 tgaataccaa aaagctcagg aaaaatggat gaaagaaaaa gaagagtcta ataaaaaggc
    18541 tcaagaagat ctcgcaaaac atgtgaaata actgttgcaa aatataagaa aggatttagt
    18601 atttctcttg aatgctgaat ccttttttac atttgtaaag aaagattcta aatgtactga
    18661 cccccaaaag ttggagcctc tttttgtcag aatagagaaa atttttgtta attttacttg
    18721 tttcctattg ctttctcagc tattatttgt tatattaaaa gtataattat tttttattta
    18781 tcagggttaa gcattgcact ttcagaggaa ggagtatttt ttaaaaagaa aatgtaaacg
    18841 cttgctaaaa aataaaagga tttagaagtt tatgaataaa ggattatttg aaaaacgttg
    18901 taaatatagt attcggaaat tttcattagg tgttgcttct gttatgattg gagctgcatt
    18961 ctttgggaca agtccggttc ttgcagatag cgtgcagtct ggttccacgg cgaacttacc
    19021 agctgattta gctactgctc ttgcaacagc aaaagagaat gatggacgtg attttgaagc
    19081 gcctaaggtg ggagaagacc aaggttctcc agaagttaca gatggaccta agacagaaga
    19141 agaactatta gcacttgaaa aagaaaaacc ggctgaagaa aaaccaaaag aggataaacc
    19201 tgcagctgct aaacctgaaa cacctaagac ggtaacccct gaatggcaaa cggtagagaa
    19261 aaaagaacaa aagggaacag tcactatccg agaagaaaaa ggtgtccgct acaaccaact
    19321 atcctcaact gctcaaaatg ataacgcagg taaaccagcc ctgtttgaaa agaagggctt
    19381 gaccgttgat gccaatggaa atgcaactgt tgatttaacc ttcaaagatg attctgaaaa
    19441 gggcaaatca cgctttggtg tcttcctgaa atttaaagat accaataata atgtttttgt
    19501 cggttatgac aaggatggct ggttctggga gtataaatct ccaacaacta gcacttggta
    19561 tagaggtagt cgtgttgctg ctcctgaaac aggatcaaca aaccgtctct ctatcactct
    19621 caagtcagac ggtcagctaa atgccagcaa taacgatgtc aatctctttg acacagtgac
    19681 tctaccagct gcggtcaatg accatcttaa aaatgagaag aagatccttc tcaaggcggg
    19741 ctcttatggc aatgaccgta cggttgtcag cgttaaaaca gacaatcaag aaggcgtaaa
    19801 agcggatgat actcctgccc agaaagaaac aggttcggtt gttgacgata gcaaggtgac
    19861 ttatgatacg atccagtcta aggtacttaa ggcagtaatt gaccaagcct tccctcgtgt
    19921 caaggaatac accttgaatg gacatacttt gccaggacag gtgcaacagt tcaaccaagt
    19981 ctttatcaat aaccaccgaa tcacccctga agtcacttat aagaaaatca atgagacaac
    20041 agcagagtac ttgatgaaga ttcgcgatga tgctcactta atcaatgcgg aaatgacagt
    20101 acgcttgcaa gttgtggaca atcaattgca ctttgatgtg accaagattg tcaaccacaa
    20161 tcaagtcact ccaggtcaaa agattgatga cgaaagaaaa ctactttctt ctattagttt
    20221 cctcggcaat gctttagtct ctgtttctag tgatcaaact ggtgctaagt ttgatggggc
    20281 aaccatgtca aacaatacgc atgtcagcgg agatgatcat atcgatgtaa ccaatccaat
    20341 gaaggatttg gctaagggtt acatgtatgg atttgtttct acagataagc ttgctgctgg
    20401 tgtttggagt aactctcaaa acagctatgg tggtggttcg aatgactgga ctcgtttgac
    20461 agcttataaa gaaacagtcg ggaatgccaa ctatgtagga atccacagct ctgaatggca
    20521 atgggaaaaa gcttataagg gcattgtttt cccagaatac acgaaggaac ttccaagggc
    20581 taaggttgtt atcactgaag atgccaatgc agacaagaaa gtcgattggc aggatggtgc
    20641 cattgcttat cgtagcatta tgaacaaccc taaaggttgg gaaaaagtta aggatatcac
    20701 agcttaccgt atcgcgatga actttggttc tcaagcacaa aacccattcc ttatgacctt
    20761 ggatggtatc aagaaaatca atctccacac agatggtctt gggcaaggtg ttctccttaa
    20821 aggatatggt agtgaaggtc acgactctgg tcacttgaac tatgctgata ttggtaagcg
    20881 tatcggtggt gtcgaagact tcaagaccct aattgagaag gctaagaaat atggagctca
    20941 tctaggtatc cacgttaacg cttcagaaac ttatcctgag tctaaatact tcaatgaaaa
    21001 aattctccgt aagaatccag atggaagcta tagctatggt tggaactggc tagatcaagg
    21061 tatcaacatt gatgctgcct atgacctagc tcatggtcgt ttggcacgtt gggaagattt
    21121 gaagaaaaaa cttggtgacg gtctcgactt tatctatgtg gacgtttggg gtaatggtca
    21181 atcaggtgat aacggtgcct gggctaccca cgttcttgct aaagaaatta acaaacaagg
    21241 ctggcgcttt gcgatcgagt ggggccatgg tggtgagtac gactctacct tccatcactg
    21301 ggcagctgac ttgacctacg gtggctacac caataaaggt atcaacagtg ccatcacccg
    21361 ctttatccgt aaccaccaaa aagatgcttg ggtaggggac tacagaagtt atggtggtgc
    21421 agccaactat ccactgctag gtggctacag catgaaagac tttgaaggct ggcaaggaag
    21481 aagtgactac aatggctatg taaccaactt atttgcccat gacgtcatga ctaagtactt
    21541 ccaacacttc actgtaagta aatgggaaaa tggtacaccg gtgactatga ccgataacgg
    21601 tagcacctat aaatggactc cagaaatgcg agtggaattg gtagatgctg acaataataa
    21661 agtagttgta actcgtaagt caaatgatgt caatagccca caatatcgcg aacgtacagt
    21721 aacgctcaac ggacgtgtca tccaagatgg ttcagcttac ttgactcctt ggaactggga
    21781 tgcgaatggt aagaaacttc ctactgataa ggaaaagatg tactacttca atacgcagac
    21841 tggtgcaaca acttggaccc ttccaagcga ttgggcagag agcaaggttt acctttacaa
    21901 gctaactgac caaggtaaga cagaagagca ggaactaact gtaaaagatg gtaaaattac
    21961 cctagatctt ctagcaaatc aaccatacgt tctctatcgt tcgaaacaaa ccaatcctga
    22021 aatgtcatgg agtgaaggca tgcacatcta tgaccaagga tttaatagcg gtaccttgaa
    22081 acattggacc atttcaggcg atgcttctaa ggcagaaatt gtcaagtctc aaggggcaaa
    22141 cgatatgctt cgtattcaag gaaacaaaga aaaagttagt ctcactcaga aattaactgg
    22201 cttgaaacca aataccaagt atgccgttta tgtcggtgtc gataaccgta gtaatgccaa
    22261 ggcaagtatc actgtgaata ctggtgaaaa agaagtgact acttatacca ataagtctct
    22321 cgcgctcaac tatgttaagg cctacgccca caatacacgt cgtgataatg ctacagttga
    22381 tgatacaagt tacttccaaa acatgtacgc cttctttaca actggatcgg acgtctcaaa
    22441 tgttactctg acattgagtc gtgaagctgg tgatcaagca acttactttg atgaaattcg
    22501 tacctttgaa aacaattcaa gcatgtacgg agacaagcat gatacaggta aaggcacctt
    22561 caagcaagac tttgaaaatg ttgctcaggg tatcttccca tttgtagtgg gtggtgtcga
    22621 aggtgttgaa gataaccgca ctcacttgtc tgaaaaacac gatccatata cacaacgtgg
    22681 ttggaatggt aagaaagtcg atgatgttat cgaaggaaat tggtcactca agacaaatgg
    22741 actagtgagc cgtcgtaact tggtttacca aactattccg caaaacttcc gttttgaagc
    22801 aggtaagacc taccgtgtaa cctttgaata cgaagcaggt tcagacaata cctatgcttt
    22861 tgtagtcggt aagggagaat tccagtcagg tcgtcgtggc actcaagcaa gcaacttgga
    22921 aatgcatgaa ttgccaaata cttggacaga ttctaagaaa gccaagaagg caaccttcct
    22981 tgtgacaggt gcagaaacag gagatacttg ggtaggtatc tactcaactg gaaatgcaag
    23041 taatactcgt ggtgattctg gtggaaatgc caacttccgt ggttataacg acttcatgat
    23101 ggataatctt caaatcgaag aaattaccct aacaggtaag atgttgacag aaaatgctct
    23161 gaagaactac ttgccaacgg ttgccatgac taactacacc aaagagtcta tggatgcttt
    23221 gaaagaggcg gtctttaacc tcagtcaggc cgatgatgat atcagtgtgg aagaagcgcg
    23281 tgcagagatt gccaagattg aagccttgaa gaatgctttg gttcagaaga aaacggcttt
    23341 ggtagcagat gactttgaaa gtttggatgc gccagctcaa ccaggtgaag gtcttgagaa
    23401 tgcctttgat ggaaacttat ctagtttatg gcatacatca tggggcggag gagatgtagg
    23461 caagcctgca accatggtct tgaaagaacc aactgaaatc actggacttc gttatgttcc
    23521 acgtggatca ggttcaaatg gtaacttgcg tgatgtgaaa cttgttgtga cagatgagtc
    23581 tggcaaggag cataccttta ctacaactga ttggccagat aacaataagt caaaagatat
    23641 tgactttggt aagacaatca aggctaagaa aattgtcctt actggtacca agacatacgg
    23701 agatggtgga gataaatacc aatctgcagc ggaacttatc tttactcgtc cacaggtagc
    23761 agaaacacct cttgacttgt caggctatga agcagctttg gctaaggctc agaaattaac
    23821 agacaaagac aatcaagagg aagtagctag cgttcaggca agcatgaaat atgcgacgga
    23881 taaccatctc ttgacggaaa gaatggtgga atactttgca gattatctca accaattaaa
    23941 agattctgct acgaaaccag atgctccaac tgtagagaaa cctgagttta aacttagctc
    24001 tttagcttcc gatcaaggta agacgccaga ttataagcaa gaaatagata gaccagaaac
    24061 acctgaacaa atcttgccag caacaggtga gagtcaatct gacacagccc tcttcctagc
    24121 aggtgttagc ctagccctat ctgctctctt tgtagtaaaa acgaagaaag actagtattt
    24181 agtaaaacct cttaacaaga ttacggaagc agtctctatc ttttccaatg aggtttatag
    24241 tacagaaaaa atctgagaag atgtcttctc agacttttgt taagcacata aatacaatag
    24301 tgctatgaca aaatcaccca gaaaatctgg gtgataaatg ttatggttgt gctggttgag
    24361 gattctgatt ttgttgatca ggggttgtat ttgattgttg cgtattattg ttaggattgg
    24421 tagtcgtact attatttgtg cttggagtgg ttgagctaga ctgtgaagtt gaactatctg
    24481 atgatgagct tgaactttca gttgatgggg gttgttgtgg agcaggtgag ctccacgtag
    24541 aacgagcacc atttttaaat acgaattctc catttctgta gagcccctct ggtatattcc
    24601 aatcctctgg attgcttcct tcagacaggt aggtcatcat agagcgataa actttagctg
    24661 caactaggaa accatctcca acgataggag ttaaacgatt cgaataacct gtccatacag
    24721 ccatagaata cttacgagta taacccacaa acatttcatc tggagctacg tagccagtgt
    24781 tcttgatata cttttcaatt tcttcgtcag tatagttaga agtacctgtc ttacctgctt
    24841 gtggaagcca tggtaggtag gctccacgtc ctgttccgta agttaaaaca gttttcatca
    24901 tttcagtcat catataggca gtagtctctt tcatagctcg tgtaccagca tcagaaaatt
    24961 ctttttcgct accatcacta aagacgattt tattgatata cattggtttg tgataaatac
    25021 caccattagc aaaagcagcg taggcagcag ccattttttc actacttgca ccatattttt
    25081 tgttggattc agttgtgtta cttgaaatgg cgtttgcata atgcatgctt ggatagtcga
    25141 taccaagacc attaaggaag gttttagctc tatctagacc gaccttattc aaagtctcaa
    25201 cggctgtgac atttcgtgat tgttgaagag catactggat tgtaatgttt ccaaagtaga
    25261 catgatccca gttgtagagt ggagtatcag tgccaggata gttataaggg acatcatgta
    25321 caatagaagc agtagagtca tagactccat attctaaagc gggagcatag tcagtgattg
    25381 gtttcattga tgatccccag tcacgattgg tttctacggc ctggttggta ccgaatgaaa
    25441 cattacttgc ttgatgacga gcaccaagtt gtgcgatgac tttaccattt gaaacatcta
    25501 cgaccgtaga tgcgacttgc aaatcatcgt cagggtaaga gacgtattga tcggagttgt
    25561 agatatccca cagatgtttt tgagcttctt ggtctacatt tgtgtaaaca tccatcccag
    25621 tagttagaag gttatagcca gtttcttgtt ctacttggtc gataacctct ttgagataat
    25681 tgtccatata tgctggatag ctattgaccg atttcaaact ttggagtcca tcagtaatag
    25741 gggtattaat agccttctca tactgttcgg cagaaatgta gccttgacct ttcatctctg
    25801 aaagtaccaa gttacgacgg tcaagggcag cttctggatg agaataggga tcgtattgat
    25861 tcggagcctg tggcattcct gctagtaggg caagttgagg taatgatagt tctcgcaaat
    25921 ctttgccgta atagttttgg gcagccgtct gcattccata gtttccgtta gacatgtaaa
    25981 ctttattcac atagtaggtc aggatttctt gttttgtggc tttttgttct aattgaacag
    26041 ccaaccaagc ttcctgggct ttacgtgaaa tggtctgatc agaagtggaa gttgagaaat
    26101 aggtcaactt aattaattgt tgggtaaggg tagatcctcc ctggagggag ttgttttgta
    26161 agttacgtaa aaaagctcct ataattcgga tggtatcgac accgcgatga tcgaaaaaac
    26221 gatggtcttc gatcgatacg atagccttga ccaaatcagt aggaatttcg ttggcctgag
    26281 cgtttactcg gcgttcagag cccagatcgg caatcagttc gtttttacta tcgaaaatct
    26341 tactagacgt ggttgcgact aatttgctct cagatagggc tggggctttg ctgacgtaat
    26401 agagaaatat gcctcctccc agcataactg ctgtgataaa taaagttaag aagcagatac
    26461 tcacatactt agctattcgc aggatagttt gtttgttcat cttgttttac cacctaataa
    26521 atgttctttg ataacattga gatagggaat ttgagggaag gcaccagcct tgatttcata
    26581 tccatattct cgaatatatc caagtggcat tgatttttgc cccttatctt gatgatagaa
    26641 gcgaatcaaa tcatatgccg gtaataagta ggtttcttgc tgagaagaaa agtgaagaag
    26701 gacaaagcag attccttgtt gagcaaggat ttgttccata tgctgaatct gatgtggatg
    26761 gaaatttttc atcggaatcg cacgtttttg ttttgtttcc ttggcttcaa agtcgatgta
    26821 atatccatta taaacgccag aatagtccgt cgttgaagct tgtcgaaaat aggcttcaac
    26881 aatcttggca cgacttcgtt gtggatagtc cactcgtacg atttgaatag gagttggttt
    26941 cttgtgtata acagccaagc cctgagacaa atagtagtcg ttggtagcat tgatcatctt
    27001 ttcaaaagac attcctcgat ttgcgaaatt tttgggttga gaaagagatg gttgtctttt
    27061 ttgtgatgaa agtttatgtg gatagttgac cataattctc cttattggta caataacatc
    27121 actctattat accataaatt tgcacagaaa gggttaaaaa tggctacagc tttggttttg
    27181 ggctattctg cttttgattt gggtttattt tctgataagg atcctcgtct taaattgata
    27241 aaaaaagcaa tacgtaaaga tttagaggct atggcagcag atggggtgtc ttggcttgtg
    27301 tttacaggga ctttgggctt tgaatattgg gttttagagg ttgctcagga gatgaaaacc
    27361 gagtacggtt tccagttggc caccattttt gcttttgaaa cccatgggga aaattggaat
    27421 gaaggcaatc agatgaaact gagtcgtttt aagcaggtag actttgtcaa atatgcttat
    27481 cctcgctatg agcacaaggg gcagttaaga gattaccagc agtttttgct ggaaaacacg
    27541 actagttcct atctttttta tgatgaagaa aatgaaacga aactagctta tttttaccaa
    27601 aagatgaaaa atcaagagga ctattttata aagagattaa catttgatca attaaatgaa
    27661 cttgctgaaa atttttccga aaattgaagc tttgaccttg atttttgttt gccttttttt
    27721 atataataat actagcaagc gagaatggag agagacatga caagtattat tttttcagca
    27781 aaagatattt ttgaacaaga gtttggacgt gaagttcgtg ggtatagcaa ggtagaagtt
    27841 gatgagtttt tagacgatgt catcaaggac tatgaaacct atgctgcctt ggttaagtca
    27901 cttcgtcagg agattgcgga tttgaaggaa gaattaactc gtaaaccgca agtgagttcg
    27961 gctccaagtc ctagtcaccc agatccaatt gatgtggctg cttcatcttc tatgacgaat
    28021 tttgatattt tgaaacgctt gaatcgtctt gaaaaagaag tattcggtaa acaaatttta
    28081 gacaatactg atttgtaatt caggtcttgt tacatgcaat ttttggataa tcgcgtgagg
    28141 agaattgctt ctcatgagga aagtccatgc tagcacaggc tgtgatgcct gtagtgtttg
    28201 tgctaggcga aaccataagc ctagggacga gaaatcgtta cggcagttga aatggctaag
    28261 tccttggata ggccagagta ggcttgaaag tgccacagtg acggagtctt tctggaaaca
    28321 gagagagtgg aacgcggtaa acccctcaag ctagcaaccc aaattttggt cggggcatgg
    28381 agtacgcgga aacgaacgta gtattctgac tgctatcagc tagagctgct agtggtagac
    28441 agatgattat cgaaggaagt ggtcctagtc atttctggaa caaaacatgg cttatagaaa
    28501 attgcatata ggttggggct gagaaatttt ctcaacctca ttttttaaag tggacatata
    28561 gaaaggtctt gcaagactgt aacatgaaaa aagaatttaa tttaattgca actgtggcag
    28621 caggtcttga agctgttgta ggacgagaag taagagagct gggctacgat tgtcaggttg
    28681 aaaatgggcg tgtgcgtttt caaggagacg tgagagcgat tatcgaaacc aacctctggc
    28741 ttcgggcagc agatcgtatc aagatcatcg taggaacgtt cccagctaag acgtttgaag
    28801 agttgtttca gggagttttt gctttagatt gggaaaatta tttaccactc ggagctcggt
    28861 tcccaatttc aaaagccaaa tgtgttaaat ctaaacttca caatgaaccc agtgttcagg
    28921 ctatttctaa gaaagctgtt gtcaagaaat tgcagaaaca ctatgctcgc ccagaagggg
    28981 ttcctctgat ggagaatggc ccagagttta agattgaggt ctctattctc aaagatgtgg
    29041 caactgtcat gattgatacg acaggctcta gcctctttaa acgtggttat cgtaccgaaa
    29101 aagggggagc ccctatcaag gaaaacatgg cggcagccat tttacaactt tctaactggt
    29161 atccagacaa gcctttgatt gatccgacat gtggttcggg gactttctgt atcgaggcgg
    29221 tcatgattgc tcgaaagatg gctccaggtc ttcgtcgctc ctttgctttt gaggaatgga
    29281 attgggtcag cgatcgcttg attcaggaag tgcgtacaga agcggctaaa aaagtagacc
    29341 gtgagcttga actggatatt atgggctgtg atatcgatgc acgtatggtg gaaattgcta
    29401 aggccaatgc tcaggcagct ggtgttgcag gagacattac ttttaagcag atgcgcgttc
    29461 aggatttacg ttctgataaa attaatggtg taatcatttc caatccgcct tatggtgaac
    29521 gtttatcaga tgatgcagga gttaccaagc tctattctga gatggggcaa gtatttgcac
    29581 cactgaaaac ttggagcaaa tttatcctga ctagtgatga agcctttgaa agcaagtatg
    29641 gtagccaagc agataagaag cgtaagttat acaacggaac cttgaaagtg gatctgtatc
    29701 aatattttgg tcagcgtgtc aaacggcaag aggtaaaata gaaagggata ctcatgagta
    29761 aaaaaagacg aaatcgtcat aaaaaagaag gtcaagaacc gcaatttgat tttgatgaag
    29821 caaaagagct aacagttggt caagcgattc gtaaaaatga agaagtggaa gcaggagtct
    29881 tgtctgagga ttccattttg gacaagtatg tcaagcaaca cagagatgaa attgaggcgg
    29941 ataagtttgc gactcgtcaa tacaaaaaag aggagttagt cgaaactcag agtctggatg
    30001 atttaattca agagatgcgt gaggctgtag aagagtcaga agcttattcg gaggaagttc
    30061 catcttctga agacatctta ccacccttgc ctctggatga tgaggagcaa ggcttggatc
    30121 ctctattact agaggatgaa aatccaacag aaatgactga agaagtggaa gaggagcaaa
    30181 atctttctcg tctggaacaa gaagagtcag aaaagaaaag caaaaaaggc tttgttttaa
    30241 ccgctttggc gcttgtatca gtgattatct gtgtcagtgc ttattatgtc tatcgtcaag
    30301 tgaatcgctc gaccaaggaa attgaaactt ctcaatcaac tacagccaat caatcggatg
    30361 tggatgattt taatacactt tatgacgcct tctacacaga cagtaataaa acggctttga
    30421 aaaatagcca gtttgataaa ctaagtcaac tcaagacctt acttgataag ctggaaggta
    30481 gtcgtgaaca tacgcttgcc aaatctaaat atgatagtct agcaacgcaa atcaaggcca
    30541 ttcaagatgt caatgcacaa tttgaaaaac cagctattgt ggatggtgtg ttggatacca
    30601 atgccaaagc caaatcggat gctaaattta cagatattaa aactggaaat acggagcttg
    30661 ataaagtgct agataaggct atcagccttg gtaagagcca gcaaacaagt acttctagct
    30721 caagttcaag tgaaactagt agctcaagca ctagtcaaac aagctcaaat acgactagtg
    30781 agacaagccc aagtagttca aatgagacta gaagtagtcg cagtgaagtc aatatgggtc
    30841 tctcgagtgc aggggttgct gttcaaagaa gtgccagtcg tgttgcctat aatcagtctg
    30901 ctattgatga tagtaacaac tctgcctggg attttgcgga tggtgtcttg gaacaaattc
    30961 tagctacttc acgttcacgt ggctatatca ctggagacca atatatcctt gaacgtgtca
    31021 atatcgttaa tggcaatggt tattacaacc tctacaagcc agatggaacc tatctcttta
    31081 cccttaactg taagacaggc tactttgtcg gaaatggcgc tggtcacgcg gatgacttgg
    31141 actactaagc agtcgttaca aaattctttc ttttcaaaag taaaaatgat aaaataaaac
    31201 aaatgaaaca agaggagtgt caaatgacaa aagctaactt tggtgtcgta ggtatggccg
    31261 taatgggtcg taaccttgcc cttaatattg aatctcgtgg ttacacagtt gctatttaca
    31321 accgtagtaa agaaaaaact gaagatgtaa ttgcttgcca tcctgaaaag aactttgtac
    31381 caagctatga cgttgaaagt tttgtaaact caatcgaaaa acctcgtcgt atcatgctga
    31441 tggttcaagc tggacctggt acagatgcta ctatccaagc ccttcttccg caccttgaca
    31501 agggtgatat cttgattgac ggtggaaata ctttctacaa agataccatc cgtcgtaatg
    31561 aagaattggc aaactcaggt atcaacttta tcggtactgg agtttctggt ggtgaaaaag
    31621 gtgcccttga aggtccttct atcatgcctg gtggacaaaa agaggcctac gaattggttg
    31681 cggatgttct tgaagaagtc tcagctaaag caccagaaga tggcaaacca tgtgtgactt
    31741 acatcggccc tgatggagct ggtcactatg tgaaaatggt tcacaatggt attgagtacg
    31801 gtgatatgca attgatcgca gaaagctatg acttgatgca acacttgcta ggcctttctg
    31861 cagaggatat ggctgaaatc tttactgagt ggaacaaggg tgaattagac agctatttga
    31921 tcgaaatcac agctgatatc ttgagccgta aagacgatga aggccaagat ggaccaatcg
    31981 tagactacat ccttgatgct gcaggtaaca agggaactgg taaatggact agccaatcat
    32041 ctcttgacct tggtgtacca ttgtcactga ttactgagtc agtgtttgca cgctacattt
    32101 caacttacaa ggaagaacgt gtacatgcta gcaaggtgct tccaaaacca gctgccttca
    32161 actttgaagg agacaaggct gaattgattg aaaaaatccg tcaagccctt tacttctcaa
    32221 aaatcatttc atacgcacaa ggatttgctc aattgcgtgt agcctctaaa gaaaacaact
    32281 ggaacttgcc atttgcagat atcgcatcta tctggcgtga tggctgtatc atccgttctc
    32341 gtttcttgca aaagattaca gatgcttaca accgtgatgc agaccttgcc aaccttcttt
    32401 tggatgaata cttcttggat gttactgcta agtatcaaca agcagtgcgt gatatcgtag
    32461 ctcttgcggt tcaagctggt gtgccagtgc caactttctc agcagctatt acttactttg
    32521 atagctaccg ttcagctgac cttccagcta acttgatcca agcacaacgt gactactttg
    32581 gtgctcacac ttaccaacgt aaagacaaag aaggaacctt ccactactct tggtatgacg
    32641 aaaaataagt aggtcagcca tggggaaacg gattttatta cttgagaaag aacgaaatct
    32701 agctcatttt ttaagtttgg aactccagaa agagcagtat cgggttgatc tggtagagga
    32761 ggggcaaaaa gccctctcca tggctcttca gacagactat gatttgattt tattgaacgt
    32821 taatctggga gatatgatgg ctcaggattt tgcagaaaaa ttgagtcgaa ctaaacctgc
    32881 ctcagtcatc atgattttag atcattggga agacttgcaa gaagagctgg aagttgttca
    32941 gcgttttgca gtttcataca tctataagcc agtccttatc gaaaatctgg tagcgcgtat
    33001 ttcggcgatc ttccgaggtc gggacttcat tgatcaacac tgcagtctga tgaaagttcc
    33061 aaggacctac cgcaatctta ggatagatgt tgaacatcac acggtttatc gtggtgaaga
    33121 gatgattgct ctgacccgcc gtgagtatga ccttttggcg acacttatgg gaagcaagaa
    33181 agtattgact cgtgagcaat tgttggaaag tgtttggaag tatgaaagtg cgaccgagac
    33241 aaatatcgta gatgtctata tccgctatct acggagcaag cttgatgtta aaggccaaaa
    33301 aagctacatt aaaaccgtgc gtggtgttgg ttacaccatg caagaataga aaagcagttg
    33361 cagttgtgta actgcttttt tgaggagttt ttatatattg acatgcagtc gggtctttgc
    33421 tacaatcgtt ttaggagggt atatagatga aaattttgaa aaaaatgatg caagttctac
    33481 tagcagtctt tttctttggt ttgctagcta caaatacggt atttgcgaat accacaggtg
    33541 gccgatttgt tgataaggat aatagaaaat attatgtaaa agatgatcat aaagcaatct
    33601 attggcataa aatagacggt aaaacctact attttggtga tggaggagag atggttgtcg
    33661 gttggcaata cttagaaatt cctggaacag gttatcgtga taatttattc gataaccaac
    33721 cagttaatga aattggcctt caggagaagt ggtactattt tggacaagat ggtgctttgt
    33781 tagaacaaac agataaacaa gtactagagg caaaaacgtc tgaaaataca ggaaaagtat
    33841 acggtgaaca atatcctcta tctgctgaaa agagaactta ttattttgat aataattatg
    33901 ttgtaaagac aggctggatt tatgaagacg gcaattggta ttatttaaat aagctaggaa
    33961 attttggcga tgattcttac aatccactac caattggtga agttgctaag ggttggactc
    34021 aagattttca tgttactatt gacattgata gaagcaaacc tgctccatgg tactacctag
    34081 atgcttccgg taagatgctt acagattggc aaaaagtaaa cggaaaatgg tattattttg
    34141 gctcctctgg ttctatggca acaggttgga aatatgtacg aggcaaatgg tattacttag
    34201 ataataaaaa tggtgatatg aagactggtt ggtatcaaga tggttcaact tggtactatt
    34261 tagatccttc taatggagat atgaaaatag gttggacaaa agtaaatgga aaatggtatt
    34321 atctcaattc aaatggagca atggttacag gtagtcaaac tatcgatggt aaagtttata
    34381 acttcgcttc atctggtgag tggatttaat gttggaggat atataaaatg aagcttttga
    34441 aaaaaactat gcaagctgga ctaacagtaa ttttctttgg tttgctagct acgaatacag
    34501 tatttgcaga taactctgaa ggatggcagt ttgtccaaga aaacggtaga acctattaca
    34561 aaaaggggga cctcaaagaa acctactggc tagtgattga tggtaagtac tattattttg
    34621 attctctatc tggagagatg gttgtcggct ggcaatatat cccgtttcca tctaaaggta
    34681 gtacaattgg tccttaccca aatggtatca gattagaagg ttttccaaag tcagagtggt
    34741 actacttcga taaaaatgga gtgctacaag agtttgttgg ttggaaaaca ttagagatta
    34801 aaactaaaga cagtgttgga agaaagtacg gggaaaaacg tgaagattca gaagataaag
    34861 aagagaagcg ttattatacg aactattact ttaatcaaaa tcattcttta gagacaggtt
    34921 ggctttatga tcagtctaac tggtattatc tagctaagac ggaaattaat ggagaaaact
    34981 accttggtgg tgaaagacgt gcggggtgga taaacgatga ttcgacttgg tactatttag
    35041 atccaacaac tggtattatg caaacaggat ggcaatatct aggtaataac tggtactacc
    35101 tccgttcatc aggagcaatg gccactggct ggtatcagga aggtaccact tggtattatt
    35161 tagaccagcc aaatggtgat atgaaaacag gatggcaata ccttggtaac aagtggtact
    35221 acctccgttc atcaggagca atggtaactg gctggtatca agatggttta acttggtact
    35281 acctaaatgc aggtaatgga gacatgaaga caggttggtt ccaggtcaat ggcaaatggt
    35341 actatgctta tagctcaggt gctttggcgg tgaatacgac cgtagatggc tactacgtca
    35401 actataatgg cgaatgggtt cggtaatgac agggcgctta taattatatt agaacactcg
    35461 cttaatcaaa gaagtgactt tgttgtactt gacctgacga ttggggagat taaaagcatg
    35521 tgaaatctac acatgctttt tcgttagtta tgagtgttga acttgactaa ccattgacaa
    35581 aagataagag caatcaaaat aagatacaaa aatatcaatg ctaactgcac cgaaacgccc
    35641 gatgctatgg acagggctaa tagggagagc agggacgggg cgagaatgtc tactgtgctc
    35701 agagcagagc ccaccgtccc catcttgtca ctggggatat gggcaaagac agctgcttgt
    35761 aggcgagggc tgagaatgcc gataatcgta gaactgatga aactggtcat gaaaattacg
    35821 atataggctt gacaaaggat actagttatt agagccaata gggaaatctc acagaaaacc
    35881 aagaggtgcg tgaaacggat atttttaaat agattactgc tggtcatatt gccaaggata
    35941 gctccacaag aaatgatgat ggagagcatg gcgaggagtt gcccaatgct cagttgtgct
    36001 agtgtctgtt ggatgaagcg gagggcaatc agccgagggg acacatccaa gatggcaatc
    36061 atactggtcg tcagaaacag tagtatgacg gtatctttta acctcaggag cacagccagc
    36121 gactgtttaa gatgttgaaa atatttctta aaactcagtg ctgtatgtga catttcaatc
    36181 cttttttcaa cctcatacaa ggtatgtcta ataacataca gacccaaaaa ggcaatgaca
    36241 aaagtcagag tgttgataag ggaaatagtt tgaatactca taacattgat aaggaatgcg
    36301 ccagccagat tggcgacaat gtggacaacc ctcatcagag actgcctgaa ccccatggca
    36361 tcatgcaggt cgtccttaat tacactgatg tagagggcgt tcatcatgta ggcagacagg
    36421 tagctcaagg tgtcagagat gaggttgatg atgacaatca taatgactga aactaatgaa
    36481 aagggcgaca agacgagaaa tactgtcaaa atagcgtaga gcaggatttt gattttggca
    36541 atccagagtg cgtgtttgac tctattttct tgaaaatctc ctagaatccc gagaaagagt
    36601 tgcgatagta ggggaaccat ctctgaaatg gaaatcagta aaatcgctag cggagcgaaa
    36661 ttgtactgag ccacataatt gataatggcg aggtagaaaa tactgtcgcc gaatgatgaa
    36721 aaacagctgt ttagcagcaa ctgtctatat atgttgtttg tccaaaataa tttcatactt
    36781 ttctccttat gtctgcacat cgaatgggaa tttattattt aatagacacc aatatacccc
    36841 atgcttccta ttccgaagtc aaacaaatct gttttaccac tatctccttg aaacatcagt
    36901 aaaccgtgat catctcgttc agaacatgct aaaattacct gttggatttt ttcatcagta
    36961 atttgttttg attgtagaca gtggtttgta gtaagctagt tattccgtgg caatatcctg
    37021 tcatcatttt tagatgatgg ttaaaaacaa attcttgata tttactaata atatctttgt
    37081 ttccgtcaca atcgtacata caaagatata agattattcc ggaaattcct tcgcaccaag
    37141 aaagttgtaa atttcctaaa ttttcagtat tagaagtaac tttttctaat aattttttta
    37201 attcagcatg gaatgtatgg agatcattat agaacataga aggttctaag actttagaat
    37261 aggcaaataa agcatatgca atttcacaat atccatgagc aaagctatag tctaaaatgc
    37321 tttctttggc tgtatctctt tgtaggaaat aatgataaat ttccccatat actattgtgg
    37381 atgaaagttt taagagtatt gtcattggta aattgatagt attttattaa tgataatagg
    37441 acaccagatt ttcccaatgc aaagtctatt tcctctagag tatcataatt ctctattaat
    37501 ttcttagcgg tgacgtttgc taattgtaga tagtattgat ttttactgat agaaaataaa
    37561 tgtaggagta accatagata gcctgcatca ccaaaagata gcctgcatca ccaaatagca
    37621 gtgagtattg atattcatat gtgtgtgtcg ttctaattgt cactgccttc ttgatgatag
    37681 agagtatttt agaatccagt tctggggggt acaatttatt tagaggaaac agtagtccag
    37741 ctattccgtg ttggaatgat aaattactta cgaaatttcc aaattctgtt gaagaagtga
    37801 tccttccttc agaatttaca gttttatcta gtaaaaatcg tagaacacta ttacattctg
    37861 ttttgtaatc atatttatgt tcctctattt ttttaatttt gactaaatta tttgtttgac
    37921 ttaaatttag tagacattgt aaccaaaata gttgctcatc tgtgagctga ccttgagtat
    37981 gtgatagttc tagtaattga ataggatcct ttagttagat tcaatcaatc tataacacaa
    38041 caacataata acacttttag gataacttat caaatattat agtaaaatga aataataaca
    38101 gga
//
LOCUS       DAVAAL010000006        36821 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVAAL010000006 DAVAAL010000000
VERSION     DAVAAL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36821
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.006"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(21..833)
                     /locus_tag="VQM60_000203"
     CDS             complement(21..833)
                     /locus_tag="VQM60_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET1080111.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            1023..2441
                     /locus_tag="VQM60_000204"
     CDS             1023..2441
                     /locus_tag="VQM60_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET1080112.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIYWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            2494..3201
                     /locus_tag="VQM60_000205"
     CDS             2494..3201
                     /locus_tag="VQM60_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET1080113.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGISIVVLVLFIIGARKTQLASFNFSFFRTKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGLVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            3296..4276
                     /gene="folP"
                     /locus_tag="VQM60_000206"
     CDS             3296..4276
                     /gene="folP"
                     /locus_tag="VQM60_000206"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET1080114.1"
                     /translation="MVKSLEGVTDMLSKTNHAKTAICGIINVTPDSFSDGGQFFTLEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEIEEEIQRVVPVIKAIRKESDVLISI
                     DTWKSQVAEAALAAGANLVNDITGLMGDEKMAHVVANAGAKVVIMFNPVMARPQHPSS
                     LIFPHFGFGQAFTEEELADFEKMPIEELMEAFFERALARAEEAGIAPENILLDPGIGF
                     GLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFHNRDT
                     ASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            4278..5600
                     /locus_tag="VQM60_000207"
     CDS             4278..5600
                     /locus_tag="VQM60_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET1080115.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVIHI
                     GGTNGKGSTIAFLKNMLEKLGLRVGVFSSPYLIHYTDQISINGESIPEARLEALMADY
                     QSLLEGESATNLQGTTEFEIITAIAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVTLLGDTLEAIAEQKAGIIKQGIPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGADYHVSHQESVVTGEVFDYTSAIRQGRFQTGLLGLHQIENAGMAIALLDTFCQE
                     VGRKLPANILIAQALEETRWPGRLEVVSRNPLMILDGAHNPHAIKALLATLQERFADY
                     SKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFDDSRATDESVLEEAAKSRNLSYQG
                     WQDFLEQKLTDKKEEKKTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            5581..6135
                     /gene="folE"
                     /locus_tag="VQM60_000208"
     CDS             5581..6135
                     /gene="folE"
                     /locus_tag="VQM60_000208"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET1080116.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            6178..6990
                     /gene="folK"
                     /locus_tag="VQM60_000209"
     CDS             6178..6990
                     /gene="folK"
                     /locus_tag="VQM60_000209"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET1080117.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(7035..7352)
                     /locus_tag="VQM60_000210"
     CDS             complement(7035..7352)
                     /locus_tag="VQM60_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET1080118.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            7709..8155
                     /gene="rplM"
                     /locus_tag="VQM60_000211"
     CDS             7709..8155
                     /gene="rplM"
                     /locus_tag="VQM60_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET1080119.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            8175..8567
                     /gene="rpsI"
                     /locus_tag="VQM60_000212"
     CDS             8175..8567
                     /gene="rpsI"
                     /locus_tag="VQM60_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET1080120.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(8829..9251)
                     /locus_tag="VQM60_000213"
     CDS             complement(8829..9251)
                     /locus_tag="VQM60_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET1080121.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            9398..9574
                     /locus_tag="VQM60_000214"
     CDS             9398..9574
                     /locus_tag="VQM60_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080122.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            9975..12188
                     /locus_tag="VQM60_000215"
     CDS             9975..12188
                     /locus_tag="VQM60_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET1080123.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFATEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(12410..12886)
                     /locus_tag="VQM60_000216"
     CDS             complement(12410..12886)
                     /locus_tag="VQM60_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET1080124.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINTFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            13109..16313
                     /locus_tag="VQM60_000217"
                     /pseudo
     CDS             13109..16313
                     /locus_tag="VQM60_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
     gene            16661..17046
                     /gene="tnpA"
                     /locus_tag="VQM60_000218"
                     /pseudo
     CDS             16661..17046
                     /gene="tnpA"
                     /locus_tag="VQM60_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(17425..18054)
                     /locus_tag="VQM60_000219"
     CDS             complement(17425..18054)
                     /locus_tag="VQM60_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET1080125.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(18064..19065)
                     /locus_tag="VQM60_000220"
     CDS             complement(18064..19065)
                     /locus_tag="VQM60_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET1080126.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(19096..19737)
                     /locus_tag="VQM60_000221"
     CDS             complement(19096..19737)
                     /locus_tag="VQM60_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET1080127.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKIIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(19756..20571)
                     /locus_tag="VQM60_000222"
     CDS             complement(19756..20571)
                     /locus_tag="VQM60_000222"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET1080128.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            20842..21276
                     /locus_tag="VQM60_000223"
     CDS             20842..21276
                     /locus_tag="VQM60_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET1080129.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            21288..22478
                     /locus_tag="VQM60_000224"
     CDS             21288..22478
                     /locus_tag="VQM60_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET1080130.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            22489..22980
                     /locus_tag="VQM60_000225"
     CDS             22489..22980
                     /locus_tag="VQM60_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET1080131.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            22995..23774
                     /locus_tag="VQM60_000226"
     CDS             22995..23774
                     /locus_tag="VQM60_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET1080132.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            23761..24579
                     /locus_tag="VQM60_000227"
     CDS             23761..24579
                     /locus_tag="VQM60_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET1080133.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            24579..24872
                     /locus_tag="VQM60_000228"
     CDS             24579..24872
                     /locus_tag="VQM60_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET1080134.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTFGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIHVEKESVMGKTKELLFK"
     gene            24894..26795
                     /locus_tag="VQM60_000229"
     CDS             24894..26795
                     /locus_tag="VQM60_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET1080135.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYLVEGDKSYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEVLTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSNKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            26855..27856
                     /locus_tag="VQM60_000230"
     CDS             26855..27856
                     /locus_tag="VQM60_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET1080136.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYVHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(27966..28301)
                     /locus_tag="VQM60_000231"
     CDS             complement(27966..28301)
                     /locus_tag="VQM60_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080137.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(28312..28554)
                     /locus_tag="VQM60_000232"
     CDS             complement(28312..28554)
                     /locus_tag="VQM60_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080138.1"
                     /translation="MKKEKRQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(28565..29107)
                     /locus_tag="VQM60_000233"
     CDS             complement(28565..29107)
                     /locus_tag="VQM60_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET1080139.1"
                     /translation="MKKETFTEKLIKRTYSISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLRILIGEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(29123..29317)
                     /locus_tag="VQM60_000234"
     CDS             complement(29123..29317)
                     /locus_tag="VQM60_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1080140.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            29483..30433
                     /gene="rsmH"
                     /locus_tag="VQM60_000235"
     CDS             29483..30433
                     /gene="rsmH"
                     /locus_tag="VQM60_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET1080141.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            30445..30762
                     /gene="ftsL"
                     /locus_tag="VQM60_000236"
     CDS             30445..30762
                     /gene="ftsL"
                     /locus_tag="VQM60_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET1080142.1"
                     /translation="MAERMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            30766..33018
                     /locus_tag="VQM60_000237"
     CDS             30766..33018
                     /locus_tag="VQM60_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET1080143.1"
                     /translation="MKWTKKVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAVFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGSKGNGITY
                     ANMMSIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRVGNIVPGTELVSQQTVDGKDVYTTLSSPLQSFMETQ
                     MDAFLEKVKGKYMTATLVSAKTGEILATTQRPTFNADTKEGITEDFVWRDILYQSNYE
                     PGSAMKVMTLASSIDNNTFPSGEYFNSSEFKIADATTRDWDVNEGLTTGGMMTFLQGF
                     AHSSNVGMSLLEQKMGDATWLDYLKRFKFGVPTRFGLTDEYAGQLPADNIVSIAQSSF
                     GQGISVTQTQMLRAFTAIANDGVMLEPKFISAIYDTNNQSVRKSQKEIVGKPVSEDTA
                     SLTRTNMILVGTDPLYGTMYNHYTGKPIITVPGQNVAVKSGTAQIADEKNGGYLVGST
                     NYIFSAVTMNPAENPDFILYVTVQQPEHYSGIQLGEFATPILERASAMKESLNLQSPA
                     KNLDQVTTESSYAMPSIKDISPGDLAEALRRNIVQPIVVGTGTKIKETSVEEGKNLAP
                     NQQVLLLSDKVEEIPDMYGWTKATAEAFSKWLNIELVFEGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            33020..34000
                     /locus_tag="VQM60_000238"
     CDS             33020..34000
                     /locus_tag="VQM60_000238"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET1080144.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLIASVLVAFFFALFSNQLSNNVGMILFILVLYGLIGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGIDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFVFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIIYVFETTSVMMQVGYFKMTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            34609..36714
                     /locus_tag="VQM60_000239"
     CDS             34609..36714
                     /locus_tag="VQM60_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET1080145.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPELLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKV"
BASE COUNT        11219 a         6525 c         8181 g        10896 t
ORIGIN      
        1 tcattgagta tcagtgtagg ctataagaat aagtcttgta gggttttggc aaccccatct
       61 tggtcgttgg taagggaaat ttgctcatct gcataaggga gtagctctgg attggcattt
      121 ttcatggcat aacctttccc agcaaaagcg agcatttcgg tatcattgtg ttcatctcca
      181 aaggcaatca aatctttttt gtcacgattc attatcttga gcaagtagtc caaagcaaag
      241 gccttgttga caccttttgg ggtacattca aggatattga gcggacctcc ccaggtattg
      301 atagaaagtt gatgctggta gaaggcgttc atgtcttttg ccaaggcata tttgtcactg
      361 gctctggtct gcaagaggat acagttaggg tccttggtta ccaattcagg ctggaattga
      421 tcttcaggct ggaaagcttc tacaccaaat agtttgggat tggcaatctc ttcattggga
      481 tttgtaatgt agaatttttt acgatattct ccagcgataa aatcggcttg aatgtcctct
      541 gaacgttgaa ccatatctag cagatatttt ttgtctacag tcaaacactt ttcaaaatcc
      601 caaacttggt ctggtaaatg agtaagggat ccgttgaagt taatcatagg agtgtctaag
      661 cccagttcac ggtaaaaatc ttttgacata cggtaagggc gacctgtcgt aataataacc
      721 tgatggcctt tttcagcaac ttttttgatg gtttttttgg taaagtcaga aatttgactg
      781 tctgagttga gcagggttcc atccaggtca actgcaataa tttttttcgt catatggacc
      841 tttctagtgt gttttcagat aagatgtctt ctattataac agaaagcagg cagaaaagca
      901 gattccaaac aaaaaatgaa attggattaa tttcttaaac aaatcaaaca aagacattga
      961 aaaagtgaat gataaatgat ataattctat tattgttcgt aaaaattaaa aggagattga
     1021 tgatggacaa attatttaaa ctaaaagaga acggtacaga cgttcgtaca gaggttctcg
     1081 ctggtttaac aactttcttt gcaatgagct atattctctt tgtaaaccca caaatacttt
     1141 cacaaacagg aatgcctgct cagggcgtct tcctagcgac gattattggt gcagtagcgg
     1201 gtaccttgat gatggctttt tatgctaact taccttatgc ccaagcgcca ggtatgggac
     1261 tcaatgcctt ctttaccttt acagttgtat tcgggcttgg ttattcttgg caagaagccc
     1321 tagctatggt cttcatctgt gggattattt cattgattat taccttgaca aatgttcgta
     1381 aaatgatcat tgaatcgatt cccaatgctc ttcgctcagc tatttcagct ggtatcggtg
     1441 tcttccttgc ctatgtaggg attaagaatg ctggactttt gaaattcacg attgatccag
     1501 gcaactatac tgttgtagga gaaggggctg acaaagctca agcaacgatt gcagcaaact
     1561 cttcagcagt tccaggattg gtcagcttta ataatccagc tgttttagtg gctcttgcag
     1621 gacttgccat tactatcttc tttgtcatca aagggattaa agggggaatt attctctcta
     1681 tcttgacaac aactgttctt gctattgcag ttggtttggt tgatttgtct agtatcgatt
     1741 ttgctaataa ccatgttggt gcagcttttg aagatttgaa gacaatcttt ggtgcagctc
     1801 ttggttcaga aggtttggga gctttggttt cagatacagc tcgcttgcct gaaactctga
     1861 tggccattct tgccttctca ttgacagata tttttgacac aattggtacc ttgatcggta
     1921 caggtgaaaa agttggtatt gtagcgacaa atggtgaaaa tcaccaatca gccaaattgg
     1981 ataaggctct ttactctgat ttgattggaa cgacagtcgg tgccattgca ggtacttcaa
     2041 acgtaacgac ttatgttgag tctgctgctg gtatcggtgc aggtggacgt actggtttga
     2101 cagccttggt tgtagctatc tgttttgcga tttcaagctt ctttagccca cttctagcga
     2161 tcgtaccaac agcggctaca gctccaatct tgattatcgt tgggattatg atgcttggta
     2221 gcttgaaaaa tatctattgg gatgatatgt ctgaagcagt tcctgccttc ttcacatcta
     2281 tctttatggg attcagctac tctatcactc aagggattgc agttggtttc ttgacttaca
     2341 ctttgactaa gcttgtcaaa ggtcaagtta aagatgttca tgccatgatt tggattttgg
     2401 atgccttgtt tatccttaac tacatcagca tggccttata atagaatgac ccagggggat
     2461 tttccccctt ttttaataca aaggagatag gtgatgaaag agaaaaatat gtggaaagaa
     2521 ttgttgaatc gtgcaggctg gattttggtc tttttacttg ccgtcctttt atatcaggtt
     2581 cccctagtgg ttacctctat tttgacttta aaagaagtag ccctgctaca gtcagggctg
     2641 atagttactg gtatttctat tgtggttctg gtgctattta ttattggagc tcgtaaaacg
     2701 caattggcaa gttttaattt ttcttttttt agaactaagg atttggcacg tttgggcttg
     2761 agttatttag tcattatctg ttcaaatata cttggttcta tcttgttgca actgtcaaat
     2821 gagacgacaa cagctaacca gtctcagatt aacgatatgg ttcagaatag ttctctgatt
     2881 tctagcttct tcttgctagc cttgcttgct ccaatttgtg aggaaatctt gtgccgtggg
     2941 cttgttccta aaaagatttt ccgaggcaag gagaacttgg gatttgtagt cggtacgatt
     3001 gtgtttgctt tattgcatca accaagtaat ttaccttctt tattgattta tggaggtatg
     3061 tcgacagttc tatcttggac ggcctacaag acccaacgtt tggaaatgtc gatcttgctt
     3121 cacatgattg ttaatgggat tgctttctgt ttgttggctc ttgtggtgat tatgagtcgg
     3181 acattaggaa tttctgttta aaagttttta tgtaggaacc gacctctttc tactagggaa
     3241 agatgaatgc aatcgtgtcc atctttttct ttttatggta aaatagaaaa ataatatggt
     3301 gaaaagcctt gagggagtga ccgatatgtt aagtaaaacc aatcatgcaa agacagctat
     3361 ttgcggaatt atcaatgtaa ccccagactc cttttcggat ggtggtcaat tttttacgct
     3421 tgagcaagcg ctccagcagg ctcgtaaatt gatagcagaa ggagccagca tgctagatat
     3481 cggtggagaa tcaactcggc cgggaagtag ctatgttgag atagaaatag aagaggaaat
     3541 ccagcgtgtt gttccagtga ttaaagcgat tcgcaaggaa agtgatgtcc tcatttccat
     3601 tgatacttgg aaaagtcagg tggcagaggc tgctttggct gctggtgcca atctagtcaa
     3661 tgatatcact ggtctcatgg gtgatgaaaa aatggcccat gtggttgcta atgctggtgc
     3721 gaaagtagtc atcatgttta atccagtcat ggctcgacct cagcacccta gctcgctcat
     3781 atttcctcat tttggttttg gacaagcttt tacagaggaa gagttagctg actttgaaaa
     3841 aatgccaatc gaagaattga tggaggcttt ctttgaacga gcactagcga gagcagagga
     3901 agctggtatt gcaccagaaa atatcctctt ggatccagga attggctttg gtttgaccaa
     3961 gaaagaaaat ctgcttcttt tacgggacct ggataaacta catcagaagg gctatccaat
     4021 ctttcttgga gtgtcgcgca agcgatttgt catcaatatc cttgaagaaa gtggttttga
     4081 agtcaatcct gagacggaac ttggtttcca caatcgagat acggcttcgg ctcatgtaac
     4141 tagtatcgct gcaagacaag gtgtagaagt ggtgcgcgtg catgatgtag ctagtcacag
     4201 gatggcagtt gaaattgcct ccgccattcg tctggctgat gaagcggaaa atttagattt
     4261 aaaacaatat aaataagatg aaagaaattg aaaacaatca gtggattgct aactaccgga
     4321 cggaccaacc gcattttggc ttggaacgaa tggtggaact gttagccttg cgtggcaatc
     4381 cccatctcaa actcaaggtc atccatatcg gagggaccaa tggcaagggg tctaccattg
     4441 cttttttgaa aaatatgcta gaaaagctag ggctgagagt tggtgttttc agttcgccct
     4501 atctcattca ttacacagac cagattagca tcaatgggga atctatccca gaggctagac
     4561 tagaagccct catggcagac tatcagtctt tgctggaagg ggaatcggct accaatttgc
     4621 aggggacaac tgagtttgaa ataattacag ctatagccta tgattacttt gcctcagagc
     4681 aagtagatgt ggctatcatg gaagtgggta tgggtggact cttggatagt accaatgtct
     4741 gtcagcctat tttgacagga attacgacta ttggattgga ccatgtaacc ctacttggtg
     4801 acaccttgga agccatagca gagcagaaag ctggtattat caaacaaggt attcccttgg
     4861 tgacaggtcg tatcgctcca gaagctttgg ctgtgattga ccgcattgcg gaagggaaag
     4921 atgcgccgag acttgcctac ggggcagatt atcatgtcag tcaccaagag agtgtggtaa
     4981 cgggtgaagt ctttgactat acaagtgcta tcagacaagg tcgcttccaa actggcctgc
     5041 ttggtttgca ccagatagag aatgctggga tggccatagc tttacttgat actttttgtc
     5101 aagaagttgg tcgaaagtta ccagctaata tcttgattgc tcaagctctg gaagaaacaa
     5161 gatggccggg gcgtctggag gtcgtgtcaa ggaacccctt gatgatcttg gacggggccc
     5221 acaatcccca tgctatcaag gctttattgg caaccttgca agaacgcttt gcggattata
     5281 gtaaggaaat cctctttact tgtatcaaaa ccaaggcctt ggaggatatg ttggacttgc
     5341 tgggggccat gccagatacc gagcttactc tgacacattt tgacgatagt cgggcgactg
     5401 atgaaagcgt gctggaagag gcagctaagt ctagaaatct cagctaccaa ggttggcagg
     5461 attttctaga gcagaaattg acagataaaa aagaagagaa aaaaacagtt aggattgtca
     5521 caggttcctt gtatttcttg agccaagtga gagcctatct gatggagagg aagaacgaga
     5581 atggatacac aaaagattga agcagctgta aaaatgatta tcgaggctgt aggagagaac
     5641 gctaatcgcg agggcttgca ggaaacacct gctcgtgtag cccgtatgta tcaagagatt
     5701 ttttcaggtc ttggtcaaac agcagaggaa catttgtcaa aatcctttga aattattgac
     5761 gataatatgg tggtagaaaa ggatatcttt ttccatacca tgtgtgaaca ccacttcttg
     5821 ccattttatg gtagagcgca cattgcctac attccagatg gtcgtgtggc aggcttgtct
     5881 aagctagccc gtacggttga agtttattcg aaaaaaccac aaattcaaga acgtttgaat
     5941 atcgaagtgg ccgatgcctt gatggactat ctaggtgcta aaggagcctt tgttgtcatt
     6001 gaggcggaac atatgtgtat gagtatgcgt ggtgttagaa aaccaggcac tgcaaccttg
     6061 acgacagtag ctcgtggtct atttgaaaca gataaggacc tccgcgacca agcttatcgt
     6121 ttaatggggc tataaaaaga atccgtttca agcggatttt tctagaaagg aatcattatg
     6181 gatcaactgc agattaagga tttggaaatg tttgcctatc atggtctttt tcctagtgag
     6241 aaagaattgg ggcagaaatt tgtcgtttca gccatcctat cctatgatat gaccaaggca
     6301 gctacagact tggatttaac agcctctgtc cattacggag aattgtgtca gcagtggacg
     6361 acttggtttc aggaaacgag tgaagatttg attgaaacgg tagcctataa actggtggaa
     6421 cgtacctttg agtcttatcc tcttgtccaa gaaatgaagt tggaactgaa aaaaccttgg
     6481 gcaccggtgc atttgtcact agatacttgc tcggtaacca ttcatcgccg caagcaacga
     6541 gcctttatcg ccctaggaag caatatggga gataaacaag caaacttgaa gcaagccatt
     6601 gacaaactgc gagctcgtgg catctatatt ctcaaagagt ccagtgtctt agcgacggag
     6661 ccttggggtg gagtggagca ggatagcttt gccaatcaag tggttgaggt ggaaacctgg
     6721 ctaccagcac aagacttgtt agaaaccttg ttagccattg agtcagagct gggacgggtg
     6781 agagaagtgc attggggacc tcgtttgatt gatttggact tgctctttgt ggaggaccag
     6841 atcctttata cagacgacct catattgcct catccttaca tagcggaacg cctttttgtc
     6901 cttgagtcct tacaggaaat tgcgcctcat tttatccatc cgatattaaa acaaccgatc
     6961 cgtaacttgt atgatgcttt gaaaaaatag aaaaactcta gttttcagtt acttgcaact
     7021 gaaggctaga gtttttaaaa taggtcattt tcttctggga ggaggatagt ttctctaccg
     7081 tccatgtcta aaaccagtac tcttggggga taacgagggt cgaaaggatg gttaaagtca
     7141 aaatcaatgg ctgtagggag gtgttgactt gaaaagtgga aggtaatctt tccttggtta
     7201 ttaagcaatt gaaactcgag ttcttcttcc aattcaaaga cattttttaa gaaatggtcg
     7261 ataatatacc aaaaagagtc aatgatgtca tcaggcaagc tggtaacaat gccaaaacta
     7321 gcagaccgca tgtgggtatt ggtaaaagcc atatctctgt ctcctttctt ttcccttatc
     7381 atacagcaaa taggattaaa aatcaagaaa aggtgatttt ttgaaaagga ttttaactac
     7441 agggaaaaag aaggaaaaaa attcccaaaa atctaccgaa gttaataggt aaattcccaa
     7501 attaacttga ttatataact ttcagttact ttgagaagtt accgaaaaat atttttcata
     7561 tctattgact tttaggggtg aaatttggta tgatagtagg cggtattgtt taccccattt
     7621 gaaaggcccc ggaaccttcc aaatactttt cgatgggaag gaacacccat caccgtaaac
     7681 aaaaatcgaa ctatatatag gagaaatcat gaacaaaaca acatttatgg ctaaaccagg
     7741 ccaagttgaa cgtaaatggt acgtagttga cgcaactgat gtaccacttg gacgtctttc
     7801 tgcagtagtt gctagcgtac ttcgcggaaa aaacaaacca acatttacac cacacactga
     7861 tacaggtgac ttcgtgattg ttatcaatgc tgaaaaagtt aaattgactg gtaaaaaagc
     7921 aactgataaa atctactaca ctcactcaaa ccacccaggt ggattgaaac aaatctctgc
     7981 aggtgaactt cgttctaaaa atgcagtacg tttgattgag aaatcagtta aaggtatgct
     8041 tccacacaat acacttggac gcgctcaagg tatgaagttg aaagtatttg ttggagctga
     8101 gcacactcat gctgcacaac aaccagaagt tcttgacatt tcaggactta tctaaggaaa
     8161 ggaacaaata aagtatgtca caagcacaat atgcaggtac tggacgtcgt aaaaacgctg
     8221 ttgcacgcgt tcgccttgtt ccaggaactg gtaaaatcac tgttaacaaa aaagatgttg
     8281 aagagtacat cccacacgct gaccttcgtc ttgtcatcaa ccaaccattc gcagttactt
     8341 caactgtagg ttcatacgac gttttcgtta acgttgtagg tggtggatac gctggtcaat
     8401 caggagctat ccgtcacggt atcgctcgtg cccttcttca agtagaccca gacttccgcg
     8461 attcattgaa acgcgcagga cttcttacac gtgactcacg taaagttgaa cgtaagaaac
     8521 caggtcttaa gaaagctcgt aaagcatcac aatttagtaa acgttaattc gaaagaatta
     8581 ctatacttat acagagcacc tttcggggtg ttcttttttt atactttctt actaaattgg
     8641 tgcaattgac acagttgttg cgactttagt cgcttacaaa tgtggctgca acctgacaag
     8701 gtcagttgcc tcaaaacgtt aatcaataca attatatcaa cgtttcaaag cactcaagag
     8761 tttacctcat gggtgctttt tttatgtttt ttgaaaaagt tcacctcaaa gtttacctta
     8821 ttttcacctt attgttttag agactgtaag gcaaattcac cgacggccat agggactaca
     8881 ttagaagtat tgacataaat ctgtgttgta cctgtgtcgc tatgtattag agcttcagaa
     8941 atggcttcta agctcattcc tgcttgttta gcgagcgttg ctccggtatg acgtagtttg
     9001 tgtggcgtag cgtgtgtaag ctctgggtgt cgttttctga tagttttcat cttattattg
     9061 agatagtcag aatgtaaagg tttattgata ttgcctttgg tatctatgta agtaaagatg
     9121 aattgttctg gattactgat gataccaaac tttgccagtt catatttttg ttgttttttc
     9181 catgaagtca gaagttgaag taagtcatta gaaatagaga aaatgggaaa tgtttccaca
     9241 ataaaacgca tattatcaga tggttaatgc gttttatatt tgggtttgag gcttataaaa
     9301 taggataagc gtactgaaaa cttagatatg acaaacaaaa taaaattttt aaaagctaca
     9361 gagaaacatg gtataatgta tctaaaggag gtagcatatg tccttagata tagataaaga
     9421 aaagatgaca atcatgggaa tagcttttga aaaccgatcc gtttttaaga gtgtttggta
     9481 tgctttaagt acaaatatga ttgaaggatg gcgacctacg gttagtgatg ttgaaaaatt
     9541 acgagatgaa gctcttgctc tggggatgac ttaatgaagc gcaactgtta tgagtcttat
     9601 gactatgttg atccggataa tttatatacc gatccgggtt cttcagttct aagaaataag
     9661 caggaaggaa aaaagcacgc gagcttgaat atcgtatgat tgctagtaag agtttgaaat
     9721 tatttatcaa tcctatctta gttaatagtt taaaagtagt ttgtttcaca actgcataag
     9781 cttagaaaag ttgaaaagtg ataactcttt aataaactct tggtttgtta aaagatattt
     9841 tgtccactac attttttcaa ttgactatta tttgtcatag atataacaga tagaacattg
     9901 cattcagaat aatgaaaaac gcatcaaaaa tggtgcgttc tgtttttatg ttaccataaa
     9961 aggaggaaaa caaattgaaa atttttaagg gagagtttta tcgaatctct gtattaacag
    10021 acaagctagt aaggttagaa tactctcaaa ctggaagttt tgaggataga acgacacaac
    10081 ttatctataa tagagatttt ggccaagttt cgttagatta tatcgagaca tcaaacgtac
    10141 tagatattat gacggactat tttcatctgc actttaataa aggagaattt aacgccgaaa
    10201 atttatttat agaattaaaa ggaaattttg ccgtatatgg tagtcgctgg tattttggtg
    10261 aatctattga aacgttaaaa ggaacagctc ggactctgga taaggcagat ggagcaatct
    10321 cgttagaaga tggaattatt agccgaaatg gtatagcctt attggatgat tctcaaggat
    10381 ttatttggga tgaacaatct ggttatattg agagagaaaa tcaaattgac ctgtatttct
    10441 ttgcctatgg gcatgattat agaggagcaa tcagagactt ttaccatttg actggttcaa
    10501 cacccttgtt gccaagatat gctttaggca attggtggag tagatattgg ccttatacgt
    10561 cggatgaata cttggattta atagacagat ttgcaacaga gaaaattcca ttatctatcg
    10621 gtgtgttaga tatggattgg catataactg acattccagc tcgttttgga agtggctgga
    10681 caggatatag ttggaataga aacttaatac caaatccaga acagttattg caacaacttc
    10741 atgatagaaa gttaaaactc tccttaaatg tccatcctgc tgatgggata cgggcttatg
    10801 aagaagctta tcctcaaatc gcaaaacggt tggggttaaa tgtagaacta gaagaacctg
    10861 ctatttttga tttttttaat ccctctttta gggaagccta ctttaaagat gttcattatg
    10921 agctagaaaa gcagggagta gatttttggt ggattgactg gcaacagggg acacaaggca
    10981 tgctagatcc actttggctt ttaaaccatt atcactatca ggatagttgt aaaaatgcag
    11041 aaggtggttt gattttatca agatatgcag gtcctggtag tcaccgctac cctgttggtt
    11101 tttcagggga tactattatc agttggaatt ccttaagatt tcaaccctat tttacagcga
    11161 cagcatctaa tatcggttat agttggtgga gtcatgatat cggtggacat atgttggggg
    11221 attatgacga agagctacaa actagatggc tacagtttgg tgtttttagt ccgattactc
    11281 gattacatag ttctagaagt ccttttaata gtaaagaacc ttggtttttt tcagaaacaa
    11341 catctaagat tatgaagaaa taccttcgtt tgagacatca gatgattcct tatctatata
    11401 ccatgaatgt aaagacacat gaggaaggtg ccccattaat cagtccaatc tattatttct
    11461 atccagagaa tgatgagagc tataatgttc caaaccaata cttttttgga acagaactga
    11521 tggtggctcc cattgtagaa aagatggatt tgacattcca atctgcaaaa gtagatgtat
    11581 ggttccctga aggtgaatgg tatgacttct tttcagagaa aaaatacaca ggtggtgtga
    11641 agttaagtgt ttatagggac atctcgacta cgcctgtgtt tgcaaaaagt ggtgcaatca
    11701 ttcccttggt tggttctgag ataggtatgg gtgttgattt acctgaggtt gtagattggt
    11761 atgtattccc agggaaacaa cattcttttg aaatgcttga agatcaaaat ggtcaaagat
    11821 ataaaacaag attatcaatc gattgggaaa tgggaatggt agagttagca ttacaaggag
    11881 attctagtat cgttccaagc aatagaaaac atagaattca ttttaaagga acgaatgtgt
    11941 ctataattga attgccaaat aagaatgata cagctaaatt tgaatggaaa gataataaaa
    12001 ggacatcttt aaatgacgaa gtttttagac tactaaagac agcttctctt ccatatgaat
    12061 taaaagatag attgttaaat caattcatca atgcgaaaaa ctctcatgac ttaatgaata
    12121 tcttgcatca tcaggataag gaattgagag ggcgtttgtt ggaaatgata tttactagcc
    12181 aaaactaatt gaaataatta gaaagtcatt tgtttagtgt atatgaagat tctgtatggt
    12241 attcgttatc actggtcaca ttgaaaatgt gaaataagca cctacaacat agactttaca
    12301 acacttcatg tttcatatca tggggtgttt ttctatttaa gtcaaagcca atcaaaaaat
    12361 ctcactattc atcaggttaa aggaaaccta atcatagtga gattgtgaat tataatagag
    12421 tttgtatcgc ctcttcaatt tgttttgggt ctgtttttga agaaaagcgt tcaaagactt
    12481 gcccatcacg accaatgaga aacttagcga aattccattc gattcgtttt cctagtgggc
    12541 cagatttatg gtcttttaac cagacataga gagggtctgc ttccttaccg ttgaccttaa
    12601 tcttggcaaa acgtgggaag gtggtttgaa aatgtaggct acagaaggtg ttgatttcct
    12661 ctgcgctgcc gggtgcttgt cccataaact gattgcaagg gaaatccaat atttcgaagc
    12721 cctgatcttg atagcgttca tagagttctt gaagtccctg gtactggggc gttaaaccac
    12781 atccagtagc agtgttgaca atcaagagaa ctttgccacg atagctatcc aagggagttg
    12841 cttggttgtt ttggttcaaa acggaaaaat cataaagtga agtcatgagg gcctcttttc
    12901 tttttctaca ttgtaacagt ttttactttt ttcgtcgagt cggatggaag atattgttga
    12961 agaataagcc ctcaatatga tagaaaccgt ttacaaataa ctttaaaaat ggtatgattt
    13021 aattaaaccg gtttactttg ttgctataaa tctctaattt gagatttagt tcctattgat
    13081 tttacaatat gtttattgga gtgtatacat gcaaacaaaa acaaagaagc tcattgtgag
    13141 tttgtcttca cttgttttat caggattttt attaaaccat tatatgacag ttggagcgga
    13201 agaaacgact acgaatacca ttcagcaaag ccagaaggaa gttcagtatc agcaaaggga
    13261 tacaaaaaat ttagttgaaa atggtgattt tggtcagacg gaggacggaa gcagtccgtg
    13321 gacaggaagc aaagctcagg ggtggtcagc ttgggtagac cagaagaata gttctgcaga
    13381 tgcctcaact cgagtcattg aggctaagga tggggctatc actatctcaa gccctgagaa
    13441 attaagggca gcggttcacc gtatggttcc tattgaagct aagaaaaagt ataaactgcg
    13501 tttcaagatt aaaacagata ataaagtcgg gattgccaaa gttcgtatca ttgagggaaa
    13561 gtggtaagga caagcgattg tggaattctg caacgacgtc aggaacaaag gactggcaga
    13621 ccattgaagc agactatagc ccgactttag atgttgataa aatcaagctg gagttattct
    13681 atgaaacagg aactgggact gtttccttta aggatattga gctggtagag gtagcagacc
    13741 agccttctga ggattctcaa acagataaac agcttgagga aaagattgat ttaccaattg
    13801 gaaaaaaaca tgttttttct cttgcggact atacttataa ggtagaaaat cctgacgttg
    13861 cttcagtcaa aaatggaatt ttagaacctc ttaaggaagg gacaaccaat gtcattgtca
    13921 gtaaagatgg caaggaagtg aaaaagattc ctttgaagat tctagcctct gttaaggatg
    13981 catacacaga ccgtttggat gactggaatg gcatcatcgc tgggaatcaa tactatgatt
    14041 ctaaaaatga acagatggcc aaattaaacc aggaattgga aggaaaggta gctgatagcc
    14101 tatccagtat ttcaagtcag gcggaccgca tctatttgtg ggaaaaattt tcaaattata
    14161 aaacgtctgc aaatctgact gccacttatc ggaaattgga ggagatggcc aagcaagtga
    14221 ccaatccttc ttctcgttat tatcaagatg aaactgtcgt tcgaacagtc agggattcca
    14281 tggaatggat gcataaatat gtctacaata gtgaaaagag cattgttggg aactggtggg
    14341 attatgaaat cggtacacct cgtgccatca acaatacctt gtctctgatg aaagaatact
    14401 tctctgatga ggaaattaaa aaatatacag atgtgattga aaaatttgta ccagatcccg
    14461 aacatttccg aaagacgact gataacccat ttaaggctct aggtggaaac ttagttgata
    14521 tgggaagagt aaaagtaata gctggtttac tgcgtaaaga tgatcaagaa atttcttcta
    14581 ccattcgctc gattgagcaa gtgttcaagt tggtagacca aggtgaaggt ttttatcaag
    14641 atggatccta tatcgaccat accaatgttg cctatacggg tgcttatggg aatgttttga
    14701 ttgatggcct gtctcaactg ttgccagtca ttcaaaagac caagaatcca atcgataaag
    14761 ataaaatgca aaccatgtac cactggattg ataaatcgtt tgctcctttg ctggtgaatg
    14821 gagagctgat ggatatgagt cgtggacgct cgatcagtcg tgcaaatagc gaggggcacg
    14881 tggccgcagt agaagtacta agagggattc accgaatagc ggatatgtct gaaggagaaa
    14941 ccaaacaacg tttgcagagt cttgtgaaga ccattgttca atcggatagt tattatgatg
    15001 tctttaagaa tttgaagact tataaggata tcagtttgat gcaatccttg ttaagtgatg
    15061 caggagtcgc aagtgttcca agaacaagtt acctatctgc ctttaacaag atggataaaa
    15121 cagccatgta caatgcagag aaagggtttg gatttggctt gtcactcttt tccagtcgta
    15181 ccttgaatta cgaacatatg aacaaggaaa ataaacgtgg ttggtatacg agtgatggga
    15241 tgttctatct ttacaatggc gatttgagtc actatagcga tggctactgg ccaacagtca
    15301 atccatataa gatgcctggt acaacagaga cggatgctaa gagagcggat agcgatacag
    15361 gtaaagtttt accgtctgct ttcgttggaa cgagcaaact agatgatgcc aatgcgacag
    15421 caaccatgga tttcaccaac tggaatcaaa cattgactgc tcataagagc tggtttatgc
    15481 taaaggataa gatcgccttt ttaggaagca atatccaaaa cacttcaaca gatactgctg
    15541 caactacaat tgaccagaga aaactggaat caggtaatcc atataaagtc tatgtcaatg
    15601 ataaagaagc ctcccttaca gaacaagaaa aggattatcc tgaaacccaa agtgtctttt
    15661 tagaatcgtc cgattcgaaa aagaatattg gttacttttt ctttaagaag agttcaatca
    15721 gtatgagtaa ggctttgcaa aagggagcct ggaaggatat caatgaagga cagtcagaca
    15781 aggaagttga aaatgaattt cttacgatta gtcaggctca taagcaaaat ggggattctt
    15841 atggctatat gctcatccct aacgtggatc gtgccacctt caatcaaatg ataaaagagt
    15901 tagaaagtag cctcatcgac aataacgaaa cccttcagtc tgtttatgat gccaaacaag
    15961 gagtttgggg cattgtgaaa tatgatgatt ctgtctctac tatttccaac caattccaag
    16021 ttttgaaacg tggagtctat atcattcgaa aagaagggga tgaatataag attgcctact
    16081 ataatcctga aacccaggaa tcagctccag atcaggaagt ctttaaaaag ctagagcaag
    16141 caactcagcc acaagtacag aattcaaaag aaaaggaaaa atctgaagag gaaaagaacc
    16201 attcggatca aaagaatctc cctcagacag gagaaggtca gtcaatcttg gcaagtctag
    16261 ggttcttgct acttggggcg ttttatctat tccgtagagg aaagaacaac taatttgttc
    16321 atcatctaga tgaattacat gaaactgtgg aaaatagaaa aatccgaaga aggccaaata
    16381 tcttcggatt tttcttttaa aaaagataag ttgatactta tcctttttta cttcattatg
    16441 tgactatagc gttttgaaac tagaatagga cgttataact gctaaaagat ttctataaat
    16501 tcatttgatt ttcctaatca atttgttcgt atcctatttc actccactat attagtccgt
    16561 ctcgctccgt gaggtgcgcg tcgaaggtta taccaaaaaa actccaaacg cgatacaata
    16621 aaggtgttca atccaattgt aaagcgaaag gagaaaaaat atggcacaaa aggctcatag
    16681 tttatctcac acaaagtgga ggtgttctat cacattgtgt tcacccctaa gtatagacga
    16741 aaagtcatct ataatcaata tagaagtagt ttaggcgaaa tatttcattg cttgtgtagt
    16801 tataaaggtg ttgaaatgat cgagggtcac ttaatgccag accatgtaca catgttagtc
    16861 agtattccgt cgaggctaag tgtttcaagt tttatggggt atttaaaagg caaaagtgca
    16921 ctcatgatgt ttgacaaaca cgccaatctc aagtacaagt ttgggaatcg gcatttctgg
    16981 gtggaaggtt attatgtaag tacagtaggg cttaataaag ctacaattaa gaaatatagt
    17041 caagattaaa gaaattatcc agtggatgat ttcttcacga gtatgaaaat ttgagaacta
    17101 gtaaagtatg atatatagta aaatgaaata agaacaggat aaaatcaatc aggacagtca
    17161 aatcgatttt taacaatgtt ttagaagtag aggtgtacta ttctagtttc aatctactat
    17221 agcactagat aaattgagtg taaaagaata tgaggatccc tttagggata gtggtaagta
    17281 ataccaaagt ctcttaaaga ggcaagtgac gagtcaagag caataaggct tgaacaacgt
    17341 gaaagccagc gtctttaggc gctggctgat gatttgggct tatagctctg agataaacca
    17401 cccgttagac gggtagttat gattttatct gagtgtaata tactgttggg caatctcgct
    17461 gatgcggtca aagttgcctt gggaagcgag tttattgagt tcgccaccaa ttccaacggc
    17521 atctgcacca gcagcgaacc attgagggat gttgtttagg ccgactcctc cggttaccat
    17581 tacggaaact tgtgggatcg gtgccttgac tgcagagata tatgctggac tgagagtact
    17641 acctgggaag agtttgatga tttcactacc ggcttcaagt gcagtcgtga tctctgtgag
    17701 ggtaatacag cctggaatgt acggtgtgct gtagagattg cacattttcg cagtttcagc
    17761 atggaaagat ggagaaacaa cgtaatttgc tccagctaga atggcatctc tagcagttac
    17821 ggcatcaagc acagtacctg caccgataca aacactctga tcgtcctgat acaagtctac
    17881 aagttccttg atgatttgtc ctgcatactg attggtatag gcgatttcaa tagctttgat
    17941 accgcccttg atacaagcaa tcgaggcttg tagtccttct tcctttgtat ttcctcgaat
    18001 gacagcgaca attttctgtt tttttagttc aataatcgta tctgatttgg tcatgtaatt
    18061 ctcctaacga atgatatctt gtgcatttgc cagtaaattt tcaatactag ttgcggaagt
    18121 ggagagatgg tctcctggaa gagtgcattt gagagttgcg ctcgcaactg caaagtcaat
    18181 ggtagttttt agggaggaat gatggagtag ttggtaaagg gcaccagata taaaggcatc
    18241 cccgctacca attcgttgat agactgcagt ttttagttgg acagactctt caaatcgttc
    18301 ttccagagca taggcttgat agacattttt gtcttgctca tcactagagc ggagggtatg
    18361 gaaaatggcc ttgaaaccat aggcttcttt taaaagtcgc atgcgatttt ccacctcttc
    18421 taggctagca ctgtctcttg gaaacatctc tagattttgg tcatcaatca tgagaggatc
    18481 aataccgaag caatagtcag taaaacgtgc aaacttagaa aattcatact tggcttctag
    18541 gactgaaatc atctttgttc tcagattgag atccattgaa acgacaattc ctcggcgctt
    18601 ggcttcttcc aagagtagga gaaggatcgc acggacctct tgaccgatag ctacggtgat
    18661 tccactaaaa tgaaaatggc taatcccctg aaagagagaa tccatatcta gcatgtttgg
    18721 ccgaatctgg ctgatactcg tatgcttacg atcgtagaaa acttcacttt gacgacaacc
    18781 aaagccgttc tccaaatagt agaggccgat tcgatcgcca agccgacaga ttgaactggt
    18841 atcgatttgg tgctgtttca agaatgtgag aaaacgatct ccaatctcgt tggcagggag
    18901 tgcggtaaag actttcgttg agatacccag ggcttgcaaa ttacaagcga tgttaatttc
    18961 tgatccgcca aaataagtcg aactggcaac atgatcgccg atactggtgg catctaatgg
    19021 tgagattcga attaatggtt ctccaaacag taatattttc gtcatccatt agttcctttc
    19081 tggtttagaa tagctttact tggctaatac ttctttcaaa taagcagcaa cttcatcatc
    19141 ttggcagttt tcaaagaagt attcttggaa gtatttgcca gagatggtgt ctttgaggaa
    19201 gtcttggtcg attattttaa ggatggtcat caaatcattg tgggtgattt gtttcacctc
    19261 gtttaagata cgagcgttgc gttgttcagg gattacacgt tctcttgggt agccaccgcc
    19321 catttcttca gcaaataagc gttcaaacat caatttgagg gtcagttctg ccccccagcc
    19381 aaatcctttg gcataaggga tagacaaggc gttaccacca ttgatttgag aataaaggta
    19441 agcgtcagtt gggtccactg ctagaccaca gacaacacca gggaagctgt ttaaagcaag
    19501 catagcccct acacccgtac cacagccggt aacaacaaag tcaactgcct ttgtatttaa
    19561 aaggatggca gccattagtc cgttctgcac ataagttaat tgactttctc cttcttctcc
    19621 acgcatacca tagttaaata attggtagcc ttttttatct gtcgcttctt ttagactatc
    19681 gtaaataatg tgattcttgc tagcttgact attttcattg attaatgcga ttttcataag
    19741 atcttctttc tatttttact caggttgttt tccgatgtag gctaagatac cgccatctac
    19801 atataggatg tggccattga caaaattgct ggcatcacta gcgagaaaga cagcagggcc
    19861 catcaaatct tcaggatttc cccaacgtgc agcaggtgtt tttgcaatga tgaactggtc
    19921 aaatgggtgg cgagaaccat cttcttgcaa ttcacgaaga ggtgctgttt gaggagtggc
    19981 aatataaccc ggtccaattc cgttacattg gatattggct ccaccgtatt cagacgcaat
    20041 gttgcgggtc aacattttca agcccccttt agcagcagca taagcgctaa ctgtttcacg
    20101 tcccagttcg ctcatcatcg aacaaatatt gataatcttt ccatgccctt tctttatcat
    20161 agaaggaata actgcctttg aaacgataaa tggtgcgttt aagtcaatat cgatgacctt
    20221 acggaaatca gcggcgctca tttcgcacat tggaactcgg cggataatac cagcgttatt
    20281 aacgaggatg tcaatgacac caacctcttg ttcgatttgc ttgaccatgg cttggatacc
    20341 gtcctcgtct gtcacgtcac agacatatcc atgggcttcg atgccaactt cacgataagc
    20401 ttcaatccct ttattgacca gatcttgatt gatatcgtta aagacaatag tagcgccggc
    20461 ttcagcgtag gatttggcaa tagcaaatcc aattccataa gaagcgccgg tgatgagagc
    20521 aatttttcct tttaaagaaa actgctcaat tgagaatgat gtatttgtca tttgatactt
    20581 cctttcaaat ttgttaagac ttctctataa ccaaattata ttctagtaaa ccggttttgt
    20641 caataaataa atatttttat ttataaaaag atttattttc cttgaaaatg gaatattttg
    20701 aaatttctgt ctattaaaat gattaaaaag ataatgaaag cgctaatatt tttttaaaaa
    20761 aactattgac aagataaaaa taaaggatta caatggggga tataaagtaa accggtaaac
    20821 ctaaacaaga aaggagaaaa gatgaaaatt gtacttgtag ggcatggaca ttttgctaca
    20881 gggatttata gttctttaca attgattgca ggcaatcaag aaaatgtgga ggcgattgac
    20941 tttgtggaag gaatgtcagc agatgaactc aagcaaaaaa tcttacttgc aatttcaaat
    21001 gaagaagaag ttttaatcct aagtgatctc ttgggaggat cgccattcaa ggtttcttct
    21061 accataatgg gagaaaatcc agccaaaaca atgaatgttc tttcgggttt gaacttagcc
    21121 atgttaatgg aagcagtctt tgctagaatg gctcatagct ttgatgaggt tgttaataaa
    21181 tcagtagtgg cggcccaggg cggagtcgta aatggtaaag aattgttttc aacggatgca
    21241 gaggaagagg aagaagattt cgaatcgggt atttaaaggg taaaagaatg ataaaaaagg
    21301 ttacgattga aaaaataaaa tcgcctgagc gcttcttaga agtaccactt ctgacgaaag
    21361 aagaagtcgg ccaggcaatc gataaggtta ttcggcagtt agaactcaac cttgactatt
    21421 tcaaggaaga tttcccgacg ccagctacct ttgataatgt ctatccaatc atggataaca
    21481 cggaatggac caatggtttc tggacaggag aactgtggtt ggcttatgaa tacagtcaac
    21541 aggatgcatt taaaaacatc gctcataaaa atgttctttc tttcctggat cgtgtcaata
    21601 agagagtaga attggatcac catgatctcg gcttcttgta cacaccgtct tgtatggctg
    21661 aatataagat aaatggagat ggagaggcta gagaagcaac cttgaaagct gcagataagt
    21721 tgattgaacg ctatcaagaa aaaggtggtt ttattcaagc ttggggagac ttgggcaaga
    21781 aagagcatta ccgtttgatt atcgactgct tgctcaatat ccaactctta ttctttgctt
    21841 atcaagaaac aggcgatcaa aaatactacg atattgcaga aagtcatttc tatgcttcag
    21901 ctaataatgt aatccgtgat gacgcttcgt ccttccacac cttctatttt gatcctgaga
    21961 caggtcaacc ctttaaaggt gtaacgagac aagggtatag tgatgattca tgctgggcac
    22021 gtggtcaatc atggggagtc tatggtattc ctttgactta tcgtcactta aaagacgagt
    22081 cctgctttga cttgtttaag ggtgtgacca attatttctt gaatcgtctg ccaaaagatc
    22141 atgtgtccta ttgggatttg atttttaatg atggtagtgc tcaatcacga gattcttcag
    22201 caacagctat cgccgtctgt gggattcatg aaatgctaaa acatctccca gaggtggatg
    22261 ctgacaaaga tatttataaa catgctatgc atgccatgct tcgttccttg atcgaacatt
    22321 atgcaaatga tcaatttacc cctggtggga caagtctcct ccacggtgtg tactcatggc
    22381 attcaggtaa aggagtggat gaaggcaata tctggggtga ctactattac ctagaagccc
    22441 ttatccgttt ctacaaagac tggaacctat attggtagga ggagaaatat gacaatgcca
    22501 aatattatta tgacccgtat cgatgaacgg ttgattcatg gacaaggaca actttgggta
    22561 aaatacctag gttgtaatac ggtcattgtt gccaatgacg aagtaagcac ggacaagatg
    22621 caacaaactc tgatgaaaac agttgtgcca gactcagttg ccatgcgttt cttccctttg
    22681 caaaaggtga ttgatatcat tcacaaggct aatcctgctc aaacgatctt tatcgttgta
    22741 aaggatgtaa aggacgcttt aaccttggta gaaggtggtg tccctatcaa agaaatcaat
    22801 attgggaaca ttcacaatgc ccctggtaaa gagcaagtga cacgctccat cttcctgggt
    22861 gaagaggaca aggcggccct caaggaatta agccaaactc atcaagtaac atttaatacg
    22921 aaaacaactc caacaggaaa tgatggagct gttcaagtca acattatgga ctatatttaa
    22981 aagaggagat cgttatgtcg attaatgtat ttcaagcgat tttaattgga ttatggacag
    23041 ctttctgttt tagtggaatg ctgttaggaa tttacaccaa tagatgtatt gttctgtcat
    23101 ttggtgtcgg aattattcta ggtgatctgc ctactgctct tgcaatggga gctattggtg
    23161 aattggctta tatgggattc ggtgttggtg ctggaggtac tgttccacca aacccaatcg
    23221 gacctggtat ctttggtacc ttgatggcta tcactagtgc tggtaaagtc agtccagaag
    23281 cggctcttgc cctctctact ccgattgctg tggcgattca attcttacaa actttcgcct
    23341 acactgtacg tgctggtgcg cctgaaacgg cgatgaagca cttgaaaaac cataatttga
    23401 agaaattcaa gttcactcta aatgcaacaa tttggttgtt tgcctttatt ggatttacct
    23461 tgggttgctt gggtgccctt tcaatggata ccttgttgaa actcgtagac tacattccac
    23521 cggtattgct tacaggtttg acagttgctg gtaaaatgct tccagctatc ggttttgcga
    23581 tgatcttgtc agtgatggct aagaaagagt tgattccctt tgtcttgttg ggatatgttt
    23641 gtgcagctta tctaaacatc ccaacaattg gtattgcaat tgtaggtact atctttgctt
    23701 tgattgaatt ttataacaag ccaaaaacag cggatcatgt ggtagaggag gaagcacacg
    23761 atgactggat ctaacaaatt aacaaaacgt gattatctta aaacgtcttt gcgggcattc
    23821 ttttgtcaaa atggatttaa ctatagtaac tatcaagggt tgggatatgc caatgtgatg
    23881 tatcctgctt tgaaaaaaca ctatggagag gatcaggaag gtttctacca agccttggaa
    23941 gaaaactgtg aattctataa taccaaccca cacttcctgc cttttattac cagcttgcat
    24001 cttgtaatgt tggaaaatgg ccgtccggca aaagaaacac gtagcatcaa gatggccttg
    24061 atgggaccat tggcaggtat tggggattct ctttctcaat tctgtttagc tccattgttc
    24121 tcaaccatcg cagcttcgtt tgctcaagaa ggcttggttg tcggtccaat cttgttcttc
    24181 cttgcgatga atacgatttt aacagcgatt aaattgtcaa ctggtctgta tggatacaaa
    24241 ctaggaacaa ctgtgattga taaactaagc gagcagatgg caacgatttc tcgtattgcc
    24301 aatattatcg gtgtaaccgt aattgctggt ttggcagcga catctgttaa gattatggtg
    24361 ccgattacct ttgctgcagg ggaagttaaa gcagacgcta aacaaagtat cgtaagtatt
    24421 cagggaatgc ttgataaggt tgctccagct cttctaccag ccctatttac acttttagtt
    24481 tactacttga tcaaagaaaa gaaatggaca acatataaac tcgttatttt aacagttatc
    24541 atcggaatta tcggaagctg gcttaagatt atagcttaat ggatacaaca ttgttttatg
    24601 gaatagtgat tgtcttggca gtgagtcccc ttttactgtc aagctttcat tctattcgtc
    24661 agcaaaagtt gcttcgcaaa cagatggagc aacgacaaga gtatttagct tctttaacat
    24721 ttggtgatga agtgttgtta ttgtctggaa ttcatggaaa aatcatttct atccaagatg
    24781 acttggtctc cttgcagatt gcaaaaggtg tggtcatcca tgtagaaaag gaaagtgtaa
    24841 tgggaaagac aaaagaactg ctttttaagt agttcttttt ctggtaatag ataatgaaag
    24901 aagaaagaag acaatttttt gaaagaatcg atggaaacca atgtcgtgat tatatcttgt
    24961 cgcactgttc aaaagactat gagaaagtca agagttccct tgaacgcttg atggacaatc
    25021 gttttatgtt tgatagtcct tgggatatgg agccttgttc aaaaatccat caaatccaac
    25081 cgatggtatg ggatcaagta tttgaagatg atccagaatg ggcttatatg ctcaatcgac
    25141 aagaatatct cttgcagttt atgatagggt atctggtaga aggagataag agctatattc
    25201 aaaagtgcaa gttctttcta tttgattgga ttgagcaggt gagagaattt tctcctcaat
    25261 ccttgatgac tagaaccttg gatacgggta ttcgttcctt tacttggttg aaactactct
    25321 tgctcctctt gaaatttgac ttgctagagg agaaagaact agagaaaatt ttggtcagtc
    25381 tagaaaagca gattgacttt atgaaaagct actatcgcgc caagtacacc cttagtaact
    25441 gggggatttt acaaacaatt ccgatgcttg ctatctatca tttcttttca gataagatgg
    25501 acctagaaga agcttatcat tttgcttcag aggagttgaa acagcaaatt gagacacaga
    25561 ttttaggaga tggaagccag tttgaacagt cgattctcta tcatgtagag gtttataaag
    25621 ccttgctgga tttgtgtctc ttgcttccag acttgcaaga tagttaccaa gagttgctgg
    25681 aaaagatggc gacctatatt caaatgatga caggcttaga tggacggact ttggcttttg
    25741 gtgatagcga ttctacagaa acgacagaaa tgttgagcct gtctgctgtg gttttgaaca
    25801 aggaagacct tcttaacggt ctggatgtta aagtcgattt gcttagcctc ttgttcctgg
    25861 ggcgagaaaa ggtcaagcga ctgcaggaat ttgaaaggag agcttggcag cctaagtcca
    25921 tgatctttga agactctgga catgtctgca ttaaggatga acatcgttat ctatttttca
    25981 aaaatggtcc gctaggaagt gcccatagcc atagtgacga gaatagtttt tgcttacagt
    26041 atcaaggcca acctattttc atagatgctg ggcgttattc ttatcgggag atatatgaac
    26101 gttatctctt aaagagtgct tggagtcatt cgacctgcat tgtagatggg aaagctccgg
    26161 aaagaatcac gggatcctgg gaatatgaat actatcctca ctccctgttt tgtcaccata
    26221 aagaaaggga gggaatgcat tatattgaag gggcttattg gtcagcagaa cctgatttgc
    26281 cttatcttca caagagaaaa atcctcatgt tggtagagga tgtctggctc ttggtagatg
    26341 acatcaggtg tcaaggtcaa catgaggtgt tgactcagtt tatccttgac aaggatgtga
    26401 cctatcaaga tgggaaaatc aatcagttga gactatggag tgaagttgat tttgatttgg
    26461 aagataccat catttctcct aaatacaatg agcttgaaag aagtaacaaa ctcaccaagc
    26521 gccaattctt tgagaatcag atgctggatt ataccatcat tgcgcatgag agttttgaaa
    26581 tcatccgtca ttctgtctac cagacagatg atcgtgaagt ggaaaatgct ctggcttttg
    26641 aagtgaaaaa tgatgaaaca gacaagctga ttctgttatt aagcgaggat attggtgtag
    26701 gtgaaaaatt gtgcctcgtt gacggaacaa aaatgcgtgg aaaatgtcta gtatatgata
    26761 aaataaatga gagaatgatt cgcttgcagt gctagaaata ggcattttga atagtgaata
    26821 tgttataata attattagta ggaggtgttt tagattggag aagaaactga ccataaaaga
    26881 cattgcggaa atggctcaga cctcgaaaac aaccgtgtca ttttacctaa acgggaaata
    26941 tgaaaaaatg tcccaagaga cacgtgaaaa gattgaaaaa gttattcatg aaacaaatta
    27001 caaaccgagc attgttgcgc gtagcttaaa ctccaaacga acaaaattaa tcggtgtttt
    27061 gatcggtgat attaccaaca gtttctcaaa ccaaattgtt aagggaattg aggatatcgc
    27121 cagccagaat ggctaccagg taatgatagg aaatagtaat tacagccaag agagtgagga
    27181 ccggtatatt gaaagcatgc ttctcttggg agtagacggc tttattattc agccgacctc
    27241 taatttccga aaatattctc gtatcatcga tgagaaaaag aagaaaatgg tcttttttga
    27301 tagtcagctc tatgaacacc ggactagctg ggttaaaacc aataactatg atgccgttta
    27361 tgacatgacc cagtcctgta tcgaaaaagg ttatgtgcat tttctcttga ttacagcgga
    27421 tacgagtcgt ttgagtactc ggattgagcg ggcaagtggt tttgtggatg ctttaacaga
    27481 tgctaatatg cgtcacgcca gtctaaccat tgaagataag catacgaatt tggaacaaat
    27541 taaagaattt ttacaaaaag aaatcgatcc cgatgaaaaa actctggtat ttatccctaa
    27601 ctgttgggcc ctacctctag tctttaccgt tatcaaagag ttgaattata acttgccgca
    27661 agttgggttg attggttttg acaatacgga gtggacttgc ttttcttctc caagtgtttc
    27721 gacgctggtt cagccctctt ttgaggaagg acaacaggct acaaagattt tgattgacca
    27781 gattgaaggc cgcaatcaag aagaaaggca acaagtcttg gattgtagtg tgaattggaa
    27841 agagtcgact ttctaaaatg aaggaaaatg acttgcaatc tctgttaaga aataaaataa
    27901 tcccacctag aacaagctag gtgggattat ttgcctatga aatgagaaat tatgggagca
    27961 agctcctaaa tcaactgttt ttgatctact tctttaacta cttgataaaa gttatagaag
    28021 taggccaaac ttgaaatgat ggttacgact aggaatattg aaaatttcca ttggacaggg
    28081 ttggttaaaa gttgtggaaa ggatatgagg agaaagaaga gggctgcgtt gaggacaggt
    28141 atccgttttg attgtatttt ctcaagtcct ttattgagcg caggaagaaa gaggagtagg
    28201 agtagtaaaa ctgtatgaga aacagctcct gaaataaggg cgaagaaaag gaaaatactg
    28261 ataaaaacat gaatgatcag tagtctagct agtgatttca taaggcacct cctaatcctg
    28321 gtctttttta gctcttgcaa tacgaagtga gtcgacaata tgtatcatca ctccgaaaaa
    28381 gaaagctccc agtatagttt taaaaatatg ttttgtattt agaagagaac tgataaaatt
    28441 tggattttca cttgttaggg tatcaatgag tggaattata aaaaatatca ctgttccata
    28501 aatcgaacct gctttcagac caggataacg taactgtctc ttttcttttt tcatagggta
    28561 cctcctaatc ctcatcttga tttttcttag tttttgcaat gcgacgggag atgaggaact
    28621 gtatgctcgc tccgaagaaa atagaaccga gaatgcttga tacaccattt cttatagtga
    28681 gaagagaatg aaaatagtcc tgaccttcac ctatgagtat cctgagaaga ggagttataa
    28741 aaaacatcca tagaccaaag aacaaacctg ctttaagacc tgggtagtgt agttgcttgc
    28801 tttctttctc attcagcata tctggttcaa tgactgtgat gcctgttttt ttcatttggt
    28861 aggtgacata gccagcagcg atgagggcaa tcactaaaat cagaggagga tagattagag
    28921 ccacttcttg agggtattta taggccagaa ggagtggaat aagatttccg aaaatcatca
    28981 gataaaagag gacgataaag acttggttcc caatactatc ggcctcacgc cgtttgtatt
    29041 cgtcaagggg accagaaata ctgtatgtgc gtttgatcag tttttcagtg aaggtttctt
    29101 ttttcatgag tttgctcctt ttttaaaaat cttcctccca aaagagactg ttgaggtcag
    29161 tttggaggct gcgggcgaga ttgagacaga gttccaaggt tggattgtac ttgtcgtttt
    29221 caatcatatt gatagtctgt ctcgagacac cgatatcctt ggcgagttcg agctgggaaa
    29281 tacccaattc cttgcgaaat tctttcacac gattcatctg gtctcctttc tgatttatgt
    29341 cgtatatatt tgactatatt atagtctttt aaacataaag tgtcaagtat ttttgacata
    29401 ttttttgaag aaatagtagt ctccttgtcc tatttgtctg acaagtgcaa gctggtcgga
    29461 tttgtggtaa aatagataag atatgacaaa agaatttcat catgtaacgg tcttactcca
    29521 cgaaacgatt gatatgcttg acgtaaaacc tgacggtatc tacgttgatg cgactttggg
    29581 tggagcaggc catagcgagt atttattaag taaattaagt gaaaaaggcc atctctatgc
    29641 ctttgaccag gatcagaatg ccattgacaa tgcgcaaaaa cgcttggcac cttacattga
    29701 gaagggaatg gtgaccttta tcaaggacaa cttccgtcat ttacaggcac gtttgcgcga
    29761 agctggtgtt caggaaattg atggaatttg ttatgacttg ggagtgtcta gtcctcaatt
    29821 agaccagcgt gagcgtggtt tttcttataa aaaggatgcg ccactggaca tgcggatgaa
    29881 tcaggatgct agtctgacag cctatgaagt ggtgaacaat tatgactatc atgacttggt
    29941 tcgtattttc ttcaagtatg gagaggacaa attctctaaa cagattgcgc gtaagattga
    30001 gcaagcgcgt gaagtgaagc cgattgagac aacgactgag ttagcagaga ttatcaagtt
    30061 ggtcaaacct gccaaggaac tcaagaagaa ggggcatcct gctaagcaga ttttccaggc
    30121 tattcgaatt gaagtcaatg atgaactggg ggcggcagat gagtccatcc agcaggctat
    30181 ggatatgttg gctctggatg gtagaatttc agtgattacc tttcattcct tagaagaccg
    30241 cttgaccaag caattgttca aggaagcttc aacagttgaa gttccaaaag gcttgccttt
    30301 catcccagat gatctcaagc ccaagatgga attggtgtcc cgtaagccaa tcttgccaag
    30361 tgcggaagag ttagaagcca ataaccgctc gcactcagcc aagttgcgcg tggccagaaa
    30421 aattcacaag taagagggaa aaagatggca gaaagaatgg aaaaaacagg tcaaatacta
    30481 cagatgcaac ttaaacggtt ttcgcgtgtg gaaaaagctt tttacttttc cattgctgta
    30541 accactctta ttgtagccat tagtattatt tttatgcaga ccaagctctt gcaagtgcag
    30601 aatgatttga caaaaatcaa tgcgcagata gaggaaaaga agaccgaatt ggacgatgcc
    30661 aagcaagagg tcaatgaact attacgtgca gaacgtttga aagaaattgc caattcacac
    30721 gatttgcaat taaacaatga aaatattcga atagcggagt aagatatgaa gtggacaaaa
    30781 aaagtaatcc gttatgcgac caaaaatcgg aaatcgccgg ctgaaaacag acgcagagtt
    30841 ggaaaaagtc tgagtttatt atctgtcttt gtttttgccg tttttttagt caattttgcg
    30901 gtcattattg ggacgggcac tcgctttgga acagatttag cgaaggaagc taagaaggtt
    30961 catcaaacca cccgtacagt tcctgccaaa cgtgggacta tttatgaccg aaatggagtc
    31021 ccgattgctg aggatgcaac ctcctataat gtctatgcgg tcattgatga gaactataag
    31081 tcagcaacgg gtaagattct ttacgtagaa aaaacacaat ttaacaaggt tgcagaggtc
    31141 tttcataagt atctggacat ggaagaatcc tatgtaagag agcaactctc gcaacctaat
    31201 ctcaagcaag tttcctttgg ttcgaagggc aatgggatta cctatgccaa tatgatgtct
    31261 atcaaaaaag agttggaaac tgcagaggtc aaggggattg attttacaac cagtcccaat
    31321 cgtagttacc caaacggaca atttgcttct agttttatcg gactagctca gctccatgaa
    31381 aatgaagatg gaagcaagag tttgctggga acctctggaa tggagagttc cttgaacagt
    31441 attcttgcag ggacagacgg tattattacc tatgaaaaag accgtgtagg aaatatcgta
    31501 ccaggtacag aactggtatc gcaacaaact gtggatggca aggatgttta tacaacattg
    31561 tctagtccgc tacaatcttt catggaaact cagatggatg cctttctaga aaaggtaaaa
    31621 ggtaagtata tgaccgcgac cttggtcagt gcaaagaccg gtgaaatcct cgctaccacc
    31681 caacgaccta cctttaatgc agatactaaa gaaggaatca ctgaggactt tgtttggcgt
    31741 gatattcttt atcaaagtaa ctatgaacca ggatcagcca tgaaggttat gacgttagct
    31801 tcttctattg ataataatac cttcccaagt ggagaatact tcaatagcag tgaatttaaa
    31861 atagcggatg cgacgactcg agattgggat gttaatgagg gtttgactac tggtgggatg
    31921 atgactttct tacaaggttt cgctcactcc agtaatgttg gaatgagtct acttgaacaa
    31981 aaaatgggag atgctacttg gttggattat ctaaaacgct ttaaatttgg ggttccaact
    32041 cgctttggct tgacagatga atacgctggt caacttccag ctgataatat tgttagtatt
    32101 gctcaaagct catttgggca aggaatttca gtgacacaaa cacaaatgct tcgtgccttt
    32161 acagctattg ctaatgatgg agttatgctg gagccaaaat ttataagtgc tatttatgat
    32221 actaacaatc agtctgtacg aaagtctcaa aaagagattg taggaaaacc tgtatctgaa
    32281 gatacagcaa gcttgactcg tactaacatg atattagttg ggacggaccc tctatatgga
    32341 actatgtata atcactacac aggaaagcca attataacag ttcctggaca aaatgtagca
    32401 gttaaatccg gtacggctca aatcgctgat gagaaaaatg gaggatactt ggttggttct
    32461 accaattata ttttctcagc tgtgactatg aatcctgctg aaaatcctga ttttatcttg
    32521 tatgtaacgg ttcaacagcc tgagcattat tcaggtatcc agttgggaga atttgccacc
    32581 ccaatcttgg agcgggcttc agctatgaaa gaatctctca atcttcaatc tccagctaag
    32641 aatttagatc aagtgacgac agaatcttcg tatgcaatgc ctagcatcaa ggatatttca
    32701 cctggtgatt tggcggaagc cttacgtcga aatattgtgc aaccaatcgt tgttggtact
    32761 ggaacaaaga ttaaagagac ttctgtagaa gaaggaaaaa atcttgcacc aaaccaacaa
    32821 gttctccttt tatcggataa ggtagaagaa attccagata tgtatggttg gacaaaagca
    32881 accgcagaag ctttttctaa gtggttaaat atagaacttg tatttgaagg ttcaggttct
    32941 actgtgcaga agcaagatgt tcgtgctaac acagctatca aggacattaa aaaaattaca
    33001 ttaactttag gagactaata tgtttatttc catcagtgct ggaattgtga catttttact
    33061 aactttggta ggaattccag cctttatcca attttataga aaggcgcaaa ttacaggcca
    33121 gcagatgcat gaggatgtca aacagcatca ggcaaaagct gggactccta caatgggagg
    33181 tttggttttc ttaattgctt ctgttttagt tgctttcttt ttcgccctat ttagtaatca
    33241 actcagcaat aatgttggta tgattttatt tatcttggtt ctctatggtt taatcggatt
    33301 tttagatgac ttcctcaagg tcttccgtaa aatcaatgag ggccttaatc ccaaacagaa
    33361 attggctctt cagcttctag gtggtgtcat tttctacctt ttctatgagc gtggtggcga
    33421 tatgctttct gtctttggct accaagtgca tctagggatt ttctatattg tcttcgctct
    33481 tttctggcta gtcggttttt caaacgcagt aaacttgaca gacggtattg acggtttagc
    33541 tagcatttcc gttgtgatta gtttgtctgc ctatggagtt attgcctatg tgcaaggtca
    33601 gatggatatc cttctagtga ttcttgccat gattggtggt ttgcttggtt tctttgtctt
    33661 taaccataag cctgctaagg tctttatggg agatgtggga agtttggctc tcggtggaat
    33721 gctggcagct atttcaatgg ctctccacca agaatggact ctcttgatta tcggaattat
    33781 ttatgtcttt gaaacaactt cggtcatgat gcaagtcggc tatttcaaaa tgactggtgg
    33841 aaaacgtatt ttccgtatga cgcctgtgca tcaccatttt gaacttggag gattgtctgg
    33901 taaaggaaat ccttggagcg agtggaaggt tgacttcttc ttttggggag tggggcttct
    33961 agcaagtctc ctgaccctag caattttgta tttgatgtaa gaatggcacc ctgatgtttc
    34021 agggtgtttt tgtgtttaaa tacacaatga aaatcaaaga acaaactaga aagctaactt
    34081 taggctgctc aaaacatagt agtttgaaac tagaatagta cacatctact tctaaaatat
    34141 tgttagaaat cgatttgact gtcctgatcg atttgtcctg ttcttatttc attttaatat
    34201 acagttttga gatgggtgat agaactgacg aagtcagctc aaaacacggt tttgaggttg
    34261 tagataagga gaagctgatg tggtttgaag aggttttcga agagtgtaaa aaaatattgg
    34321 tcagttaaag tcaaaactat tgaccttttc tgaccaatgt agtatattat gaacgtaagg
    34381 taaatgaaag ccttactaga acacttattt aaagtaaaat agaaagaggt gggtctatgt
    34441 ttcatctaga aatcttcaga agtaaagatg gtctactcct gcttgaaaaa gaaaaaccag
    34501 aaatagtacg tagggtagcg gtttagctag tctaaatttt ttaaaacaat ggtcaaagat
    34561 agtcaatatc agtaatcata actaagtaaa caaaaagagg taaagaatat gaataacaac
    34621 tttaataatt ttaataacat ggatgattta tttaaccaat tgatgggtgg tatgcgagga
    34681 tatagttctg aaaatcgccg ttacttaatt aatggacgcg aagtcacacc tgaggaattt
    34741 gctcactatc gtgcgactgg tcaattacca ggaaatgcag aaactgatgt gcaaatgcca
    34801 caacaggcat caggtatgaa acaagacggt gtccttgcaa aactaggtcg aaacttgaca
    34861 gcagaagcgc gtgagggcaa gttggatcct gttatcggac gaaacaagga aattcaagaa
    34921 acatctgaaa tcctctcacg ccgcaccaag aacaatcctg ttttggtcgg agatgcaggt
    34981 gttggtaaga cagcagttgt cgaaggtcta gcgcaagcca ttgtgaacgg agatgttcct
    35041 gctgctatca agaacaagga aattatttct attgatatct caggtcttga ggctggtact
    35101 caataccgtg gtagctttga agaaaatgtc caaaacttag tcaatgaagt gaaagaagca
    35161 gggaatatta tcctcttctt tgatgaaatt caccaaattc ttggtgctgg tagcactggt
    35221 ggagacagtg gttctaaagg acttgcggat attctcaagc cagctctctc tcgtggagaa
    35281 ttgacagtga ttggggcaac aactcaagac gaataccgta acaccatctt gaagaatgct
    35341 gctcttgctc gtcgtttcaa cgaagtgaag gtcaatgctc cttcagcaga gaatactttt
    35401 aaaattcttc aaggcattcg tgacctctat caacaacacc acaatgtcat cttgccagac
    35461 gaagtcttga aagcagcggt ggattattct gttcaataca ttcctcaacg tagcttgcca
    35521 gataaggcta ttgaccttgt cgatgtaacg gctgctcact tggcggctca acatccagta
    35581 acagatgtgc atgctgttga acgagaaatc gaaacggaaa aagacaagca agaaaaagca
    35641 gttgaagcag aagattttga agcagctcta aactataaaa cacgcattgc agaattggaa
    35701 aagaaaatcg aaaaccacac agaagatatg aaagtgactg caagtgtcaa cgatgtggct
    35761 gaatccgtgg aacgaatgac aggtatccca gtatcgcaaa tgggagcttc agatatcgaa
    35821 cgtttgaaag atatggctca tcgcttgcaa gacaaggtga ttggtcaaga taaggccgta
    35881 gaagctgtag ctcgtgctat ccgtcgtaac cgtgctggtt ttgatgaagg aaatcgccca
    35941 atcggcagct tcctctttgt aggatctact ggggttggta agacggagct tgctaagcaa
    36001 ttggcactcg atatgtttgg aacccaggat gcgattatcc gtttggatat gtctgaatac
    36061 agtgaccgca cagctgtttc taagctaatt ggtacaacag caggctatgt gggttatgat
    36121 gacaatagca ataccttaac agaacgtgtt cgtcgcaatc catactctat cattctcttg
    36181 gatgaaattg aaaaggctga tcctcaagtt attacccttc tcctccaagt tctagatgat
    36241 ggtcgtttga cagatggtca aggaaataca gtaaacttca agaacactgt cattattgcg
    36301 acctcaaatg ctggatttgg ctatgaagcc aacttgacag aagatgcgga taaaccagaa
    36361 ttgatggacc gtttgaaacc tttcttccgt ccagaactcc tcaaccgctt taatgcagtc
    36421 atcgagttct cacacttgac taaggaagac ctttctaaga ttgtagattt gatgttggct
    36481 gaagttaacc aaaccttggc taagaaagac attgacttgg tagtcagtca agcggctaaa
    36541 gattatatca cagaagaagg ttacgacgaa gtcatggggg ttcgtcctct ccgtcgcgtg
    36601 gttgaacaag aaattcgtga taaggtgaca gacttccact tggatcattt agatgctaaa
    36661 catctggaag cagatatgga agatggcgtt ttggttattc gtgagaaagt ctaagacaga
    36721 attttgagga taaaaaagaa ggagccagct gaaaaaaact ggttcctttt gaggtatgac
    36781 gggcatgtcg tatagtagaa gtgtattatt ctagtttcaa t
//
LOCUS       DAVAAL010000007        36553 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVAAL010000007 DAVAAL010000000
VERSION     DAVAAL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36553
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.007"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            9..89
                     /locus_tag="VQM60_000240"
     tRNA            9..89
                     /locus_tag="VQM60_000240"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Tyr,seq:gta)
     gene            94..164
                     /locus_tag="VQM60_000241"
     tRNA            94..164
                     /locus_tag="VQM60_000241"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Trp,seq:cca)
     gene            179..251
                     /locus_tag="VQM60_000242"
     tRNA            179..251
                     /locus_tag="VQM60_000242"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:His,seq:gtg)
     gene            262..333
                     /locus_tag="VQM60_000243"
     tRNA            262..333
                     /locus_tag="VQM60_000243"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:294..296,aa:Gln,seq:ttg)
     gene            343..426
                     /locus_tag="VQM60_000244"
     tRNA            343..426
                     /locus_tag="VQM60_000244"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Leu,seq:caa)
     gene            533..1810
                     /locus_tag="VQM60_000245"
     CDS             533..1810
                     /locus_tag="VQM60_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET1080146.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYFFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1886..3370
                     /locus_tag="VQM60_000246"
     CDS             1886..3370
                     /locus_tag="VQM60_000246"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET1080147.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDTSYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTVELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEISPIRHKTTVAAADMQAAVEAYLGL"
     gene            3431..4711
                     /locus_tag="VQM60_000247"
     CDS             3431..4711
                     /locus_tag="VQM60_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET1080148.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLLGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4715..5335
                     /locus_tag="VQM60_000248"
     CDS             4715..5335
                     /locus_tag="VQM60_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET1080149.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSDFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5501..6643
                     /locus_tag="VQM60_000249"
     CDS             5501..6643
                     /locus_tag="VQM60_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET1080150.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVIVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQETTPAEKQETQASPQAASAVEVTTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6966..7391
                     /locus_tag="VQM60_000250"
     CDS             6966..7391
                     /locus_tag="VQM60_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET1080151.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAIQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7402..7803
                     /locus_tag="VQM60_000251"
     CDS             7402..7803
                     /locus_tag="VQM60_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET1080152.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKIHLKVDSNLANPF"
     gene            7878..8522
                     /gene="pcp"
                     /locus_tag="VQM60_000252"
     CDS             7878..8522
                     /gene="pcp"
                     /locus_tag="VQM60_000252"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET1080153.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(8550..9407)
                     /locus_tag="VQM60_000253"
     CDS             complement(8550..9407)
                     /locus_tag="VQM60_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET1080154.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9537..10679
                     /gene="tgt"
                     /locus_tag="VQM60_000254"
     CDS             9537..10679
                     /gene="tgt"
                     /locus_tag="VQM60_000254"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET1080155.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10780..12597
                     /locus_tag="VQM60_000255"
     CDS             10780..12597
                     /locus_tag="VQM60_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET1080156.1"
                     /translation="MRIKWFSLIRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGNYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQSRSN
                     GQLRGMVFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSILATIVGV
                     RQTTSLVKQLDKIWDLRKTLLVFAGGFGFLLILTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTPNVQEPKMISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPVLQTLRPLPYIHAILATSTGILVFIVFLVTLLAPQV
                     GAFETDLTVNGLKQAASNIAQTKVMAERAEADSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSRTTKTANEIMLNNSQNKFLPKMVVIATGVNNPENYKEDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITVADWNQVAKEHPEIWAG
                     TDQVHFGSDTSTIEAGAKLYAETIATALQTAQDKPVKSK"
     gene            12750..13901
                     /gene="nagA"
                     /locus_tag="VQM60_000256"
     CDS             12750..13901
                     /gene="nagA"
                     /locus_tag="VQM60_000256"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET1080157.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14064..15122
                     /locus_tag="VQM60_000257"
     CDS             14064..15122
                     /locus_tag="VQM60_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET1080158.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15273..15638
                     /locus_tag="VQM60_000258"
     CDS             15273..15638
                     /locus_tag="VQM60_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET1080159.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15714..16655
                     /locus_tag="VQM60_000259"
     CDS             15714..16655
                     /locus_tag="VQM60_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET1080160.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16603..17619
                     /locus_tag="VQM60_000260"
     CDS             16603..17619
                     /locus_tag="VQM60_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET1080161.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17621..17947
                     /gene="comGC"
                     /locus_tag="VQM60_000261"
     CDS             17621..17947
                     /gene="comGC"
                     /locus_tag="VQM60_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HET1080162.1"
                     /translation="MKKMMTFLKKSKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLQADGRITEEQAKAYKEYHDKNGVA
                     NRKVND"
     gene            17940..18344
                     /locus_tag="VQM60_000262"
     CDS             17940..18344
                     /locus_tag="VQM60_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET1080163.1"
                     /translation="MIKAFTMLESLLALSLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTISNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18349..18609
                     /locus_tag="VQM60_000263"
     CDS             18349..18609
                     /locus_tag="VQM60_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080164.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVNINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18572..19033
                     /locus_tag="VQM60_000264"
     CDS             18572..19033
                     /locus_tag="VQM60_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET1080165.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRFYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19011..19424
                     /locus_tag="VQM60_000265"
     CDS             19011..19424
                     /locus_tag="VQM60_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET1080166.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKKSEEKPEKKENS"
     gene            19561..20148
                     /locus_tag="VQM60_000266"
     CDS             19561..20148
                     /locus_tag="VQM60_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET1080167.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKEILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKAEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVNSQILYSAEDLFQGNYSEFFLIVAQKSLA"
     gene            20209..21162
                     /locus_tag="VQM60_000267"
     CDS             20209..21162
                     /locus_tag="VQM60_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET1080168.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21213..22403
                     /locus_tag="VQM60_000268"
     CDS             21213..22403
                     /locus_tag="VQM60_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET1080169.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22552..22923
                     /gene="rnpA"
                     /locus_tag="VQM60_000269"
     CDS             22552..22923
                     /gene="rnpA"
                     /locus_tag="VQM60_000269"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET1080170.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            22898..23722
                     /locus_tag="VQM60_000270"
     CDS             22898..23722
                     /locus_tag="VQM60_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET1080171.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23741..24727
                     /locus_tag="VQM60_000271"
     CDS             23741..24727
                     /locus_tag="VQM60_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET1080172.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSRTFYVTINVNDYVEHRAEVLQTYAQKL
                     ATRVLEEGRNHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24778..25401
                     /locus_tag="VQM60_000272"
     CDS             24778..25401
                     /locus_tag="VQM60_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET1080173.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMIALRLYGNPSDF
                     GISLEVSFIERKKDEQTLGKQAKVLEIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYQVTRG"
     gene            25530..26987
                     /locus_tag="VQM60_000273"
     CDS             25530..26987
                     /locus_tag="VQM60_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET1080174.1"
                     /translation="MEVVSNVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLIIIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLAAYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27010..27291
                     /locus_tag="VQM60_000274"
     CDS             27010..27291
                     /locus_tag="VQM60_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET1080175.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQRDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27367..27852
                     /locus_tag="VQM60_000275"
     CDS             27367..27852
                     /locus_tag="VQM60_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET1080176.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            27869..28534
                     /locus_tag="VQM60_000276"
     CDS             27869..28534
                     /locus_tag="VQM60_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET1080177.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            28538..29401
                     /locus_tag="VQM60_000277"
     CDS             28538..29401
                     /locus_tag="VQM60_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET1080178.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYGTGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29403..30107
                     /locus_tag="VQM60_000278"
     CDS             29403..30107
                     /locus_tag="VQM60_000278"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET1080179.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30290..31963
                     /locus_tag="VQM60_000279"
     CDS             30290..31963
                     /locus_tag="VQM60_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET1080180.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32075..33166
                     /gene="ulaG"
                     /locus_tag="VQM60_000280"
     CDS             32075..33166
                     /gene="ulaG"
                     /locus_tag="VQM60_000280"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET1080181.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33280..35256
                     /gene="tkt"
                     /locus_tag="VQM60_000281"
     CDS             33280..35256
                     /gene="tkt"
                     /locus_tag="VQM60_000281"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET1080182.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35373..35672
                     /gene="yajC"
                     /locus_tag="VQM60_000282"
     CDS             35373..35672
                     /gene="yajC"
                     /locus_tag="VQM60_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET1080183.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            35718..36146
                     /locus_tag="VQM60_000283"
     CDS             35718..36146
                     /locus_tag="VQM60_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET1080184.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36148..>36553
                     /locus_tag="VQM60_000284"
     CDS             36148..>36553
                     /locus_tag="VQM60_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080185.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQ"
BASE COUNT        11060 a         6458 c         8286 g        10749 t
ORIGIN      
        1 tatattttgg aagggtagcg aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg
       61 gttcgggggt tcgaatccct ccccttccat tttacgggca tagtttaaag gtagaactaa
      121 ggtctccaaa accttcagtg tgggttcaat tcctactgcc cgtgttaata gaattatggc
      181 gggtgtggtg aagtggttaa cacaccagat tgtggctctg gcatgcgtgg gttcgatccc
      241 catcactcgc ctattttata ttggggtata gccaagcggt aaggcaaggg actttgactc
      301 cctcatgcgt tggttcgaat ccagctaccc cagttactat ttgccggcgt ggcggaattg
      361 gcagacgcgc tggactcaaa atccagtgtc cgcaaggacg tgccggttcg accccggccg
      421 ccggtatagt atagtgttag gaacgttgtt attcttcgtt ccttttttat attatttttg
      481 gtataattat agttattcaa attttattta gattaagaaa gtgtagggta gtatgtcttg
      541 ttctatcgat ttattaaaac atcggtattt gaaaaatatt aaagaaaatc ctgaattgtt
      601 tgtcggaatt gagttggagt atcctgttgc aagtttagaa ggggatgcta cagatgttga
      661 agttataaag gatctatttc attatttagt ttctactttg gatctcaccg tagcaaaggt
      721 agatgatttt ggcaatctga tccagttagt agatccgata agtcaggatg ctattttatt
      781 tgaagtttcc tatacaacga ttgagtttgc atttggtaag gctgaaacga ttcaagaggt
      841 cgaaaatcgt ttcaataatt atatgaatgt aattcagaga aagttagctg aatcaaatca
      901 tgctattgtt ggctgtggta tccatcccaa ctgggataaa aatgagaatt gtccagtggc
      961 ttatccacgc tatcagatgt tgatggatta tttgaatttg agtagaaata ttattaaatc
     1021 agatttacat catttccctg aatatggtac ttttatctgt gggagccagg ttcagctgga
     1081 tatttcaaaa accaactact tacgggtgat taatgctttt actcaaattg aagcggctaa
     1141 ggcttatttc tttgcaaact ctgaattttc gggtgcggat tgggatacga aaatttcaag
     1201 ggatattttc tgggaagaat ctatgcatgg tatctatcca gagaatgttg gggtcaatgc
     1261 tagactcctt aatgatgaag ctgatttttt tgactatcta aatcattctg cgatttttac
     1321 tgcggaacgt gatgggcaga cctattattt ttatcctatt caggctgggg actatttggc
     1381 tacgcctgaa atccaagcat ttgctctgaa tggggatgag gttattattt ccccccaaga
     1441 gaaggatttt gaaactcatc gtagttacca gtaccaagac ttaacgactc gaggaacagt
     1501 tgagtttcgt agtgtgtgta cacagccact tgataggact tttgcttctg cagcttttca
     1561 cttgggatta ttggttaatt tagacaagtt agaagcttac ttagaaacag cacctttctt
     1621 taaagtattt ggttatgatt acaagtcttt aaggagacaa ttttctaaga aaaatcttac
     1681 agatgaggaa gaaactacga ttattgaatt ttccaaagac ttactcctac tagctgagga
     1741 gggactagtg gtgagaaata aggaagaaat gacctattta cagcctttga gagaagaatt
     1801 gagcctataa tttctcttat aaagggagaa ttttctgaaa aatcatgata taatggacga
     1861 gactatagat aaaggataga gagtaatgac attagtttat caatcaacgc gtgatgccaa
     1921 caatacagta actgctagcc aagcaatttt gcaaggtttg gcgacggacg gcggtttgtt
     1981 tacaccggtt acttatccaa aggtagattt ggactttgac aaattgaaag atacttctta
     2041 ccaggaagtt gctaagctag ttttgtcagc atttttagat gactttacag ctgaggagtt
     2101 ggactactgt atcaacaatg cctacgatag caaatttgat actccagcta ttgcaccatt
     2161 agtgaaatta gatgggcaat acaatttgga acttttccat ggttcaacga ttgcctttaa
     2221 ggatatggcc ttgtctattt tgccatactt tatgacgact gctgctaaga aacatggttt
     2281 ggagaacaag attgttatct tgacagcaac atctggtgac acggggaaag ctgctatggc
     2341 ggggtttgcg gatgtgcctg gtactgagat tatcgtcttt tatccaaagg atggtgtcag
     2401 caagattcaa gagttacaaa tgaccactca aactggcgac aatactcatg ttattgctat
     2461 tgatggtaac tttgacgatg cgcaaacaaa tgtgaagcac atgtttaacg acgtggctct
     2521 tcgtgaaaaa ttgactacca acaagttgca attttcatca gctaactcta tgaacattgg
     2581 tcgtctggtg ccacaaattg tttattatgt ttatgcttac gctcaattgg tcaagactgg
     2641 tgagatcgtg gctggtgata agattaactt cacagtacca acaggaaact ttggaaatat
     2701 cttggctgcc ttttatgcca aacaaatcgg tctcccagtt ggtaaattga tctgtgcttc
     2761 aaatgacaac aatgttttga cagacttctt caagacacgt gtttacgaca agaaacgtga
     2821 gtttaaggta acaaccagcc catctatgga tatcttggta tcttcaaact tggagcgctt
     2881 gattttccat cttttgggaa ataatgctga aaagacagtt gaacttatga atgccttgaa
     2941 cacgcaagga caatataagt tgacagactt tgatgcagag attttggacc tctttgcagc
     3001 tgaatatgcg actgaggaag aaacggcagc agagatcaag cgtgtttgtg agttagattc
     3061 ttatatcgag gaccctcata cagctgttgc ttcagcagtt tataaaaaat accaatcggc
     3121 cactggagat gtaactaaga cagtgattgc ttcaacagct agtccataca agttcccagt
     3181 agttgcagta gaagctgtaa ctggaaaagc aggtttaaca gactttgaag ccttggctca
     3241 attacatgaa atctcaggcg ttgcagtgcc accagcagtt gatgggcttg aaatatctcc
     3301 aattcgtcac aagacaacag tggcagctgc tgacatgcaa gcagcggttg aggcttattt
     3361 aggactttaa gacagaggga gcaaactcgg ttgggaaacc aactgagttt cttttcatca
     3421 ggaggagaga ttgtttaaga aaaataaaga cattcttaat attgcattgc cagctatggg
     3481 tgaaaacttt ttgcagatgc taatgggaat ggtggacagt tatttggttg ctcatttagg
     3541 attgatagct atttcagggg tttcagtagc tggtaatatt atcaccattt atcaggcgat
     3601 tttcatcgct ctgggagctg ctatttccag tgttatttca aaaagcatag ggcagaaaga
     3661 ccagtcgaag ttggcctatc atgtgactga ggcgttgaag attaccttac tattaagttt
     3721 ccttttagga tttttgtcca tcttcgctgg gaaagagatg ataggacttt tggggacgga
     3781 gagggatgta gctgagagtg gtggactgta tctatctttg gtaggcggat cgattgttct
     3841 cttaggttta atgactagtc tgggagcctt gattcgtgca acgcataatc cacgtttgcc
     3901 tctctatgtt agttttttat ccaatgcctt gaatattctt ttttcaagtc tagctatttt
     3961 tgttctggat atggggatag ctggtgttgc ttgggggaca attctgtctc gtttggttgg
     4021 tcttgtgatt ttgtggtcac aattaaaact gccttatggg aagccaactt ttggtttaga
     4081 taaggaactg ttgaccttgg ctttaccagc agctggagag cgacttatga tgagggctgg
     4141 agatgtagtg atcattgcct tggtcgtttc ttttgggacg gaggcagttg ctgggaatgc
     4201 aatcggagaa gtcttgaccc agtttaacta tatgcctgcc tttggcgtcg ctacggcaac
     4261 ggtcatgctg ttggcccgag cagttggaga ggatgattgg aaaagagtcg ctagtttgag
     4321 taaacaaacc ttttggcttt ctctgttcct catgttgccc ctgtccttta gtatatatgt
     4381 cttgggtgta ccattaactc atctctatac gactgattct ctagcggtgg aggctagtgt
     4441 tctagtgaca ctgttttcac tacttggaac ccctatgacg acaggaacag tcatctatac
     4501 ggcagtctgg cagggattag gaaatgcacg cctccctttt tatgcgacaa gtataggcat
     4561 gtggtgtatc cgcattggaa caggatatct gctggggatt gtgcttggtt ggggcttgcc
     4621 tggtatttgg gcagggtctc tcttggataa tggttttcgc tggttatttc tacgctatcg
     4681 ttaccagcgc tatatgagct tgaaaggata ggaaatgcaa aaaacagctt ttatttggga
     4741 tttagacggg actttattgg actcttacga agcgatttta tcagggattg aggagacttt
     4801 tgctcagttt tctattcctt atgataagga gaaggtgaga gagtttatct tcaagtattc
     4861 ggtgcaagat ttgcttgtgc gggtggcaga agatagaaat ctggatgttg aggtgctaaa
     4921 tcaggtgcgt gcccagagtc tggctgagaa gaatgctcag gtagttttga tgccaggtgc
     4981 gcgtgaggtg ctagcttggg cagacgaatc aggaattcag cagtttatat atactcataa
     5041 ggggaacaac gcttttacca ttctcaagga cttgggggtg gaatcctatt ttacagagat
     5101 tttaaccagt cagagtgact ttgtgcggaa gccaagtcca gaagcggcta cctatctgct
     5161 agataagtat cagttgaatt ctgataatac ttattatata ggggatcgga ctctggatgt
     5221 ggaatttgcc cagaatagtg ggattcaaag tatcaacttt ttagagtcta cttatgaagg
     5281 gaatcacagg attcaagcgt tagcagatat ttcccgtatt tttgagacta agtgataaaa
     5341 agattgtgtc agttttgtga cagagaccta acaaactatt tcaagtaacc tagtttgtta
     5401 caaggaatag acagttctgt taaataggcc cgagagggct ttttttctac attttttgtg
     5461 ttatgataga caggtactca tttgaaagga atttgaaaga atgaagaaaa gaatgttatt
     5521 agcgtcaaca gtagccttgt catttgcccc agtattggca actcaagcag aagaagttct
     5581 ttggactgca cgtagtgttg agcaaatcca aaacgatttg actaaaacgg acaacaaaac
     5641 aagttatacc gtacagtatg gtgatacctt gagcactatt gcagaagcct tgggtgtaga
     5701 tgtcacagtg cttgcgaatc tgaacaaaat cactaatatg gacttgattt tcccagaaac
     5761 tgttttgaca acgactgtca atgaagcaga agaagtaaca gaagttgaaa tccaaacacc
     5821 tcaagcagac tctagtgaag aagtgacaac tgcgacagca gatttgacca ctaatcaagt
     5881 gatcgttgat gatcaaactg ttcaggttgc agacctttct caaccaattg cagaagctcc
     5941 aaaagaagta gcatcaagtt cagaagttac aaagacagtg attgcttctg aagaagtggc
     6001 accatctacg ggcacttctg tcccagagga gcaaacggcc gaaacaagca gtgcagttgc
     6061 agaagaagct cctcaggaaa cgactccagc tgagaagcag gaaacacaag caagccctca
     6121 agctgcatca gcagtggaag taactacaac aagttcagaa gcaaaagaag tagcatcatc
     6181 aaatggagct acagcagcag tttctactta tcaaccagaa gagacgaaaa taatttcaac
     6241 aacttacgag gctccagctg cgcccgatta tgctggactt gcagtagcaa aatctgaaaa
     6301 tgcaggtctt caaccacaaa cagctgcctt taaagaagaa attgctaact tgtttggcat
     6361 tacatccttt agtggttatc gtccaggaga cagtggagat cacggaaaag gtttggctat
     6421 cgactttatg gtaccagaac gttcagaatt aggggataag attgcggaat atgctattca
     6481 aaatatggcc agccgtggca ttagttacat catctggaaa caacgtttct atgctccatt
     6541 cgatagcaaa tatgggccag ctaacacttg gaacccaatg ccagaccgtg gtagtgtgac
     6601 agaaaatcac tatgatcacg ttcacgtttc aatgaatgga taaacccgac ttgataacat
     6661 cattttgacg aatgagatct agctttcgtg atggcaagcg attctcgttc gttttttctt
     6721 tgtcatactc ttcgaaaatc tcttcaaacc acgtcagttt tatctgaaac ttcaaagctg
     6781 tgctttgagc aacctgcgac tagcttccta gtttgctttt tgattttcat tgagtatcaa
     6841 tttgaatgga aaatggaaag ttatcatctt gtaatgagtt aagcaacatt cttgcaatct
     6901 attttacttt atatcacaat taattagtca aatattgata aatcaataaa aagagagggg
     6961 aagaaatgct agagattcaa gatttactgt atcaactccg cttgtctgag caagcgagta
     7021 cgcaattgtt tgaaaaaagg cttgggatta gtttgacacg gtatcagatt ttactgtttt
     7081 tgctggagca ttctccttgt aaccaaatgg cgattcagga gcgtttgaaa attgatcagg
     7141 ctgctttgac acggcatttc aagattttgg aaacggaagg tttggtggag cgtcatcgta
     7201 atcctgaaaa tcagcgggaa gtgttggtag aggctgcgaa gtatgccaag gagcagttag
     7261 tggtgaatcc ccctctgcaa catatcaagg ttaaggaaga gatggaaagt atcttaacag
     7321 agtttgagag aacagaactc agccgtttat taaataaatt ggttttgggt attgaaaata
     7381 tagaaattta aggagaaata gatgtcaatt attacaatta ttttaacaac gatcgttgct
     7441 ttggagcatt tttacatttt ttatttggaa agtattgcta cgcgatcaga tgcgactagt
     7501 cgtgtattta atatggaaaa ggaagaattg gctcatccgt cagtaagttc attgttcaaa
     7561 aatcaaggga tttataaggc tctgctagga gtctttctct ggtatggcat ttatttctca
     7621 cagaatttag aaattgtgac tatttttgtc ttatttgtga ttggtgctgc gacttacggc
     7681 tctttaacag cggataaaaa aattattttg aagcaaggtg gatcagctat tttggccttg
     7741 attagtattt tactctttaa aatacacttg aaggtcgatt ctaatctcgc taatcctttt
     7801 taatccagaa taagggaaat atgttatact tgtttttaag aaaaaagtct cattgaattg
     7861 gttttgagga gttagaaatg aaagtattag tgacaggttt tgagcccttt ggaggggaaa
     7921 agggcaatcc aactttggag gccattaaag gtttaccagc tgaaatccat ggtgctgagg
     7981 tccgttggct agaggtgccg acagtttttc acaaatctgc tcaagtattg gaagaagaga
     8041 tgaatcgtta tcaacctgac tttgtccttt gtattgggca agctggtgga agaactagtg
     8101 tgacacctga acgagtggcc attaatcaag acgatgcacg catttctgat aacgaagata
     8161 atcaaccgat tgaccgtccc attcgcccag atggtgcttc ggcctacttt agtagtttgc
     8221 cgattaaagc gatggttcaa gctataaaaa aagagggctt accggcctct gtttccaata
     8281 cggcagggac ttttgtctgc agccatttga tgtatcaggc tctctatttg gtagaaaaga
     8341 aatttccata tgttaaggca ggttttatgc atattcctta tatgatggaa caggtggtga
     8401 acagaccgac tactccaact atgagtttag tggatattcg gcgagggata gaagcagcaa
     8461 tcggcgctat gatagaacat ggagatcagg aactcaagtt ggtaggcgga gaaactcatt
     8521 gatagaaaaa agcttgaggg gaaaaacctt caagcttttg gacgttttcg agccaatact
     8581 gctcggtaaa acataatttt agtgcattgg atataaggta ggagtgaaaa actagcaatg
     8641 ccaaaggtaa tccaattgag gaagtaccaa ggaagaagct gtaaatctag gacaaagtgc
     8701 tggaacttgt agcccttcat aaaggaacgg ctagttttta ggattcgtct tggtgggacc
     8761 tgtcctaggt ctagactata acagagaaga aattccacct gtgaataggc ataatactgt
     8821 ggaatataga ggatatttcc tacaatgatc aagatgagac ttgcaagaaa gtagagtcca
     8881 aagaccatga ggaaacgctc ggtttcaact gatgagagat ctagatttgg aaactcagga
     8941 tgtagggtga cgaatttttt ggctaaaaag ctactataaa agaggagata aatcccaagt
     9001 aaattaggga tactccataa aaagagatag aaacgtttga gaagtagggt caaaaaggtt
     9061 tgagaaaagc gctcctcatc aaagagagct aggctgtttt ttacagatgg ctccgtttta
     9121 gaatctttca tgagtgtcag tgttgcatag acggaactgg tcaaaagaat agtcccgata
     9181 aaggagacta gtagaggaaa gaggtaggct tgaagtattt ggccaagtat gctgaaaaat
     9241 ggctgttcta aaacagtccc gtggatccga gataagggat taagaaaact agataagatg
     9301 accagcatac tgggaaggat atagaggaga aagagacggg gggtgtcagc ctgaaaatgt
     9361 tttgactcct gacgaattgt ttttaaatca atttttggat agttcattct cttattatac
     9421 catagttctt atacatagtt cgtgacagtt cctacttttt ttgataaaat catacagtgt
     9481 gtccttgggc acactgtatg aactgggact gtctttccca gcttcggagg taaaaaatgt
     9541 cagattcacc aatcaaatat cgtttgatta agaaagaaaa acacacagga gctcgtctag
     9601 gagaaatcat cactccccac ggtacctttc cgacacctat gtttatgcca gttgggacac
     9661 aagccactgt caaaactcag tcacctgaag aattgaagga gatgggttcg ggaattatcc
     9721 tatcaaacac ttatcatctc tggcttcgcc ctggagatga actcattgca cgcgctggtg
     9781 gtctccacaa gttcatgaat tgggaccagc ctatcttgac agatagtggt ggttttcagg
     9841 tttattcttt agcagatagc cgtaatatca cagaagaagg agtaaccttt aaaaatcatc
     9901 taaatggttc taagatgttc ctatccccag aaaaagccat ctctattcag aataatctgg
     9961 gttcagacat catgatgtcc tttgatgaat gtcctcagtt ttatcaacct tatgactacg
    10021 ttaagaaatc gatcgagcgt accagccgtt gggctgagcg tggtttgaag gctcaccgtc
    10081 gtccacatga ccaaggtttg tttggaattg tgcaaggtgc aggatttgaa gaccttcgcc
    10141 gccaatcagc tcatgatctt gtcagcatgg atttctcagg ctactctatc ggtggtttgg
    10201 cagtgggaga aacccatgaa gagatgaatg cggtcttgga ctttacaact caactgctgc
    10261 ctgaaaataa acctcgttat ctgatgggtg tgggagcgcc agatagcttg atcgatgggg
    10321 tcattcgtgg ggtggatatg tttgactgtg tcttaccgac tcgaattgct cgtaacggga
    10381 cttgtatgac cagtcaagga cgtttggttg tgaaaaatgc ccagtttgct gaggacttta
    10441 cgccactgga tcctgagtgt gactgctaca catgtaataa ctatacacgc gcttaccttc
    10501 gtcacctgct caaggctgat gaaacctttg gtatccgctt gactagctac cacaatcttt
    10561 acttcttgct taacctgatg aagcaagtgc gacaagccat catggatgac aatctcttgg
    10621 aattccgtga gtattttgtg gaaaaatatg gctataataa gtcaggacgc aatttctaaa
    10681 acggaataga tataagctaa aaatcctaag ttttctcttg ggatttttct tctttttttg
    10741 atagaataaa gtgtataatg aaagggagaa taaactcgta tgcgcattaa atggttttcc
    10801 ttgattagga ttacaggttt actactggta ctcttgtatc atttctttca gaccatcttt
    10861 cctggaggat ttttcggggt agatgtcttt ttcacatttt caggtttcct gattacggct
    10921 ctactcatcg aagaattttc taaaaatcat gagattgact tgataggatt ttttaggaga
    10981 cgcttttatc ggattgtgcc acctgtggtt ttgatggtct tggtgaccat gccctttacc
    11041 tttctagttc ggcaagatta tgtggctgga attgggggtc agattgcggg tgtcttaggc
    11101 tttatgacca acttctatga acttctaaca ggtgggaatt atgaatctca gttcattcca
    11161 catttgtttg ttcataattg gagcttggct gttgaggttc actactatat tctatgggga
    11221 ttggcagttt ggttcttatc caagcagtct agatcaaatg gtcagttgag agggatggtc
    11281 tttctcttat ctgctactgc cttcttgatt agcttcttct ccatgtttat cggtagtttt
    11341 ctagtgacct cttattcttc tgtttatttc tccagtttaa ctcatgtcta tccattcttt
    11401 ttgggaagta tcctagcaac gattgtaggc gttcgtcaga cgacttccct agttaagcaa
    11461 ttggataaaa tctgggattt acgcaagacc cttttagttt ttgcaggagg ttttggcttc
    11521 ttactcattt tgaccttctt cgtcaaattt acctatctct ttgcctatct tattggcttc
    11581 ttgcttgcca gtcttgcagc ccttgctatg attctggcgg cgcgtgtctt acatgaaaag
    11641 acacctaacg tgcaggagcc aaagatgatt agctttttag cagatactag ctatgcagtt
    11701 tatctcttcc attggccttt ctatatcatt ttctcacagt tgacatcaaa tcttcttgct
    11761 gtattactga ctctgatttg ttcttatggc tttgccagtc tttcatttta tgtgttggaa
    11821 ccttggattg caggaaagaa cacacctgtt ttacaaactc ttcgtcccct gccttatatt
    11881 cacgcaattc ttgcaacaag tacaggaatc ttggttttca ttgttttctt agtgacttta
    11941 ttggcaccac aagtgggagc gtttgagaca gacttaactg tcaatggctt gaagcaagct
    12001 gctagtaata ttgcccaaac aaaagtgatg gcagaacgag cagaagctga tagtttgggg
    12061 attgctgatg gtactatgtt aattggtgat tcagtggctt taagggcaaa tacagccctg
    12121 cagacagctc ttcctggagc acaaattaac gcgcaggtca gcagaacaac caagactgcc
    12181 aatgaaatca tgctaaacaa tagccagaat aaatttttac ctaagatggt ggtcattgcg
    12241 actggggtaa ataatcctga aaattacaag gaagactggg acagtattgt aaaaaatctt
    12301 cctaagggac accatatgat tttagtcact ccttatgagg gggataagac aaaagagacc
    12361 tatgccatcg ttgagaaggc tgctgcctat atgagagaat tggcagagaa gacaccttat
    12421 atcacagttg ctgattggaa tcaagttgcg aaagagcatc cagaaatttg ggcaggaaca
    12481 gaccaagtcc atttcggaag tgacactagc actattgaag caggagcaaa attgtatgca
    12541 gagaccattg ccacagcctt gcaaacagct caagacaagc cggttaaatc aaaataactt
    12601 gtattaaaaa agagaagagt tggagaaatc cagctctttt aaaatatctc caaaaaatag
    12661 gtctatacca tttacaaata aaaaagaaag gtttataatg taattgacat aataaattgt
    12721 agaatcaatc ttttaaggag gttaacatta tgcctaacta tattaaagcg gatcagtttt
    12781 tctacccaca cggagttcgt cgaggtggtt acttggaact tgtggacggc aagtttggga
    12841 aacatgtaga gcagattcct gaaggggctg aggtgattga ctatacaggt tatagcattg
    12901 ccccaggtct tgtggatact catattcatg gatatgcagg tgtagatgtg atggacaaca
    12961 acattgaagg tacattgcat actatgagtg aaggacttct tagtaccggt gttaccagtt
    13021 tcttacccac aactttaaca gccacttatg agcaattgct tgcagtcact gaaaatcttg
    13081 gaaaccatta taaagaagca acaggtgcta agattcgtgg gatttattat gaaggtccat
    13141 atttcacaga aacttttaag ggggcacaaa atccaactta tatgagagac ccgggtgttg
    13201 aggagtttca ttcttggcaa aaagcggcaa atggcttgct taataaaatt gcccttgcac
    13261 cagaacgtga tggggtggaa gactttgttc gtacagttac gggcgaaggt gtgacggttg
    13321 ctcttggaca ttcaaacgcg acttttgatg aagccaaaaa agcagtcgat gctggagcga
    13381 gtgtttgggt gcatgcctac aatggaatgc gtgggttgac tcaccgtgaa ttgggtatgg
    13441 ttggagccat gtaccaattg ccacatacct atgcagagtt gatctgtgat ggtcaccacg
    13501 tagatccaaa ggcttgcgaa attcttatca aacaaaaagg aacagaaaac atcgctctta
    13561 tcacagactg tatgacagct gggggattgg aagacggaga ttatatgttg ggagaattcc
    13621 cagttgtcgt tgcaaatgga actgcacgcc tcaaatcgac aggtaacttg gcaggttcta
    13681 tcctcaaact caaagatggt ttgaagaatg tggtcgaatg gggaattgcg aatccgcatg
    13741 aagcagtcat gatggccagc ttcaacccag ctaaatccgt tcacatcgat gacgtctgtg
    13801 gccaaatccg tgaaggctac gacgctgact tcatcgtatt agataaagat ttggaattgg
    13861 tagcaaccta tctagatggc gtaaaacgtt atcaagcata agagagaagg cagaagttag
    13921 agactagctt ctgctttttt atacagggta ctttactggt aaataaaaag ttaaaaaaat
    13981 cacaaaaaaa gcttgaagaa acaaagaaaa aggcttacaa ttgaactata aatattacaa
    14041 ataaaaaaac ggaggagtgc tttatgaaag cctatactta tgttaaacca ggacttgctt
    14101 cttttgttga tgtagacaaa ccagttattc gcaagccaac agacgctatt gtgcgtattg
    14161 taaaaaccac tatttgtgga acagacctcc atattatcaa aggggatgtt cctacttgcc
    14221 aaagtggtac cattcttggc cacgaaggga ttgggattgt tgaagaagtt ggggaaggag
    14281 tttccaactt caaaaaaggt gacaaggtct tgatttcttg cgtctgtgcc tgtggtaaat
    14341 gctactactg taaaaaagga atttatgctc actgtgaaga cgaagggggc tggattttcg
    14401 gtcacttgat tgatggtatg caggctgaat atctacgtgt ccctcatgca gataatactc
    14461 tttaccatac tccagaagac ttgtcagatg aagctttggt tatgctgtca gacattctgc
    14521 ctactggata tgaaattggt gtcttaaaag ggaaagtaga acctggttgc agcgtagcca
    14581 ttattggttc aggtccagtt ggattggctg ctcttttaac agcccaattc tattcaccag
    14641 ctaaattgat tatggtagac ctagacgata accgcttgga aactgcccta tcattcggtg
    14701 cgactcataa ggttaattct tcagaccctg aaaaagccat taaagaaatt tatgatttga
    14761 cagatggtcg tggtgtggat gtcgctatcg aagctgttgg tattcctgca acatttgatt
    14821 tctgtcaaaa gattatcggt gtagacggaa cggttgccaa ctgtggtgtg catggtaaac
    14881 cagttgaatt cgatttagat aaactttgga ttcgcaacat caatgtaaca actggtttgg
    14941 tatctacaaa tacgactcca caattgttga aagcgcttga aagtcataag attgaaccag
    15001 aaaaattggt aactcactat ttcaaactca gtgaaattga aaaagcctac gaagtcttca
    15061 gtaaggcagc agaccaccat gccattaagg tcattatcga aaacgatatc tcagaagcct
    15121 aagtagtaaa aatatttttg tacataagta aatagaaatt cagtcatcca tcagatggct
    15181 ggatttttta tcaaaaaatt aagaaatgag catatttctt tccttgtctg gcggaattgg
    15241 ttataatata cggtacaaag gaatgaatga atatgtatcg tgttatagaa atgtacggag
    15301 attttgaacc gtggtggttc ttagaaggtt gggaagaaga tattgtagca agtagaaaat
    15361 ttgaccagta ttatgatgct ctcaaatact acaaaacttg ctggtttaga ttggaacaag
    15421 aatcgcctct ttataaaagt agaagcgact tgatgaccat tttttgggac ccggaagacc
    15481 aacgctggtg tgatgaatgt gatgagtatt tacaacaata ccattctttg gctcttttgc
    15541 aggatgagca ggttatccca gacgaaaaac tacgctcagg ctatgaaaaa caaaccagtc
    15601 aggaaaggaa tcgttcttgc cgtatgaaat taaaatagag aaaagtaact tttttggagt
    15661 tgcttttttt atttttctaa ctctttgcga atagtatagg tgaggaggta agtatggttc
    15721 aagaaattgc acaagaaatc attcgttcag ctcggaaaaa agggacgcag gatatctatt
    15781 ttgtccctaa gttagacgcc tatgagcttc atatgagggt aggagacgag cgctgtaaaa
    15841 ttggtagcta tgattttgaa aagtttgcag ccgttatcag tcactttaag tttgtggcgg
    15901 gtatgaatgt gggagaaaaa agacgtagtc aactgggttc ctgtgattat gcctatgacc
    15961 agaagatagc gtctctacgt ttatctactg taggcgatta tcgggggcat gagagtttgg
    16021 ttatccgttt gttgcacgat gaggagcagg acctgcattt ttggtttcag gatattgaag
    16081 aattaggcaa gcagtacagg caacgggggc tctatctttt tgctggtccg gttgggagtg
    16141 gtaagacgac cttgatgcat gaattgtcca agtcactctt taaaggacag caagttatgt
    16201 ccatcgaaga tcctgtcgaa atcaagcagg acgacatgct tcagttgcag ttgaacgaag
    16261 caatcggcct aacctatgaa aatctaatca aactttcctt gcgtcatcga ccagatctct
    16321 tgattatcgg agaaattcgt gacagcgaga cggcgcgtgc agtggtcaga gctagtttga
    16381 caggtgcgac agtcttttca accattcatg ctaagagtat ccgaggtgtt tatgagcgtc
    16441 tgctggagtt gggtgtgagt gaagaagaat tggcagttgt tttgcaagga gtctgctacc
    16501 agagattaat cgggggagga ggaatcgttg actttgcaaa cagagattat caagaacacc
    16561 aagcagccaa gtggaatgag caaattgacc agcttcttaa agatggacat atcacaagtc
    16621 ttcaggctga gacggaaaaa attagctaca gctaagcaaa aaaatatcat caccctattt
    16681 aacaatctct tttctagcgg ttttcatctg gtggagacca tctccttttt agataggagt
    16741 gctttgttgg acaagcagtg tgtaacccag atgcgtgtgg gcttgtctca ggggaaatca
    16801 ttctcagaaa tgatggaaag tttgggatgt tcaagtgcta ttgtcactca gttatcccta
    16861 gctgaagttc atggcaatct ccacctgagt ttgggaaaga tagaagaata tctggacaat
    16921 ctggccaagg tcaagaaaaa attaattgaa gtagcgacct atcctttgat tttgctgttt
    16981 tttcttctct taattatgct ggggctacgg aattacctgc tcccacaact ggatagtagc
    17041 aatattgcca cccgaattat cggtaatctg ccacaaattt ttctaggcat ggtagggctt
    17101 gtttccgtgc ttgccctttt agcactaact ttttataaaa gaagttctaa gatgagtgtt
    17161 ttttctatct tagcacgcct tccctttatt ggaatctttg tgcagaccta cttgacagcc
    17221 tattatgcac gtgaatgggg gaatatgatt tcacagggaa tggagctgac gcagattttt
    17281 caaatgatgc aggaacaagg ttcccagctc tttaaagaaa tcggtcaaga tctggctcaa
    17341 accctgaaaa atggccgtga attttctcag acgataggaa cctatccttt ctttaggaag
    17401 gaattgagtc tcatcataga gtatggggaa gttaagtcca agctgggtag tgagttggaa
    17461 atctatgctg aaaaaacttg ggaagccttt tttacccgag tcaaccgcac catgaatttg
    17521 gtgcagccac tggtttttat ctttgtggca ctgattatcg ttttacttta tgcggcaatg
    17581 ctcatgccca tgtatcaaaa tatggaggta aatttttaaa atgaaaaaaa tgatgacatt
    17641 cttgaaaaaa tctaaggtta aagcttttac attggtggag atgttggtgg tcttgctgat
    17701 tatcagcgtg cttttcttgc tctttgtacc taatctgacc aagcaaaaag aggcagtcaa
    17761 tgacaaagga aaagcagctg ttgttaaggt ggtggaaagc caggcagaac tttatagctt
    17821 ggaaaagaat gaagatgcta gcctaagcaa gttacaagca gatgggcgaa tcacggaaga
    17881 acaggctaaa gcttataaag aataccatga taaaaatgga gtagcaaatc gtaaagtcaa
    17941 tgattaaggc ctttaccatg ctggaaagtc tcttggcttt gagtcttgtg agtatccttg
    18001 ccttgggctt gtccggctct gttcagtcca cttttgcggc agtagaggaa cagattttct
    18061 ttatggagtt tgaagaactc tatcgggaaa cccaaaaacg cagtgtagcc agtcagcaaa
    18121 agactagtct gaacttagat gggcagacga ttagcaatgg cagtcaaaag ttgccagtcc
    18181 ctaaaggaat tcaggcccca tcaggccaaa gtattacatt tgaccgtgct gggggcaatt
    18241 cgtccctggc taaggttgaa tttcagacca gtaaaggagc gattcgctat caattatatc
    18301 taggaaatgg aaaaattaaa cgcattaagg aaacaaaaaa ttagggcagt gattttactg
    18361 gaagcagtag tcgctctagc tatctttgcc agcattgcga ccctcctttt gggacaaatt
    18421 caaaaaaata ggcaagagga agcaaaaatc ttgcaaaagg aagaagtctt gagggtagct
    18481 aagatggcct tgcagacagg tcaaaatcag gtaaacataa acggagttga gattcaggtg
    18541 ttttctagcg aaaagggatt ggaggtctac catggttcag aacagttgtt ggcaatcaaa
    18601 gagccataag gtcaaggctt ttaccttgtt agaatccctg cttgccctgc tcgtcatcag
    18661 tggaggctta ctcctttttc aagctatgag tcagctcctc atttcagaag ttcgctacca
    18721 gcaacaaagc gagcaaaagg agtggctctt gtttgtggat caacttgagg cagaattaga
    18781 ccgttcgcag ttcgaaaaag tggaaggcaa tcgtttctat atgaagcaag atggcaagga
    18841 catcgccatc ggtaagtcaa agtcagacga ttttcgtaaa accgatgcta gtggacgggg
    18901 gtatcagcct atggtttatg gcctcaaatc agctcagatt acagaggaca atcaactggt
    18961 tcattttcgt ttccagtttc aaaaaggctt agaaagggag ttcatctatc gtgtggaaaa
    19021 agaagaaagt taaggcaggt gttctcctct atgcagtcac catggcagct atctttagtc
    19081 ttttgttgca attttacttg aaccgacaag tcgcccacta tcaagactat gctttgaata
    19141 aagaaaaatt ggttgctttt gccatggcta aacgaaccaa ggataaagcc gagcaagaaa
    19201 gtggggaaca ggtctttaat ctaggtcagg taagctatca aaacaagaaa acaagcttgg
    19261 tgacgactgt tcgtacggat aagagtcaat atgagtttct gtttccttca gtcaaaatca
    19321 aagaagagaa aagagataaa aaggaagagg tagcgactga ttcaagcgaa aaagcggaga
    19381 agaaaaaatc agaagagaag cctgaaaaga aagagaattc ctagtcaatt caactataat
    19441 gcgttgaatc cagaatagtc cactgtagtt tctagaaaat tgctggaaat ggatgttaag
    19501 ctccaattca tttgtttata tcttatttca gtttactata ctttgtgcta aactaaagat
    19561 atgaaacatg attttaacca caaagcagaa actttcgatt cccctaaaaa tatcttcctc
    19621 gcaaacttgg tatgtcaagc agtcgagaaa cagattgata ttttatcaga caaagaaatt
    19681 ttagatttcg gtggtggcac gggtctatta gccttgcccc tagccaagca ggctaagtca
    19741 gttactcttg tagacatttc tgagaaaatg ttggagcaag ctcgtttgaa agcggagcag
    19801 caagcaatca agaatatcca gtttttggag caagatttac cgaaaaatcc cttggagaaa
    19861 gagtttgatt gccttgctgt tagtcgggtt cttcatcata tgcctgattt ggatgcggct
    19921 ctctcactgt ttcatcaaca tttgaaggaa gatgggaaac tcatcattgc tgattttacc
    19981 aagacagaag ctaatcatca tggatttgat ttagctgaac tggaaaacaa gctaattgag
    20041 catggttttt catctgtgaa tagtcagatt ctctatagcg ctgaagacct gtttcaagga
    20101 aattactcag aattcttttt aatagtagcc caaaaatcac tcgcctagtc agggagtgat
    20161 ttttctataa ggatggaaaa aagaagggaa atttgataag ataggaatat ggattttgaa
    20221 aaaattgaac aagcttatac ctatttacta gagaatgtcc aagtcatcca aagtgatttg
    20281 gcgaccaact tttatgacgc cttggtggag caaaacagca tctatctgga tggtgaaact
    20341 gagctaaacc aggtcaaaga caacaatcag gcccttaagc gtttagcact acgcaaagaa
    20401 gaatggctca agacctacca gtttctcttg atgaaggctg ggcaaacaga acccttgcag
    20461 gccaatcacc agtttacacc agatgccatt gctttgcttt tggtgtttat tgtggaagag
    20521 ttgtttaaag aggaggaaat tactatcctc gaaatgggtt ctgggatggg aattctaggc
    20581 gctactttct tgacctcgct tgataaaaag gtggattact tgggaatgga aatggatgat
    20641 ttgctgattg atctggcagc tagcatggca gatgtaattg gtttgcaggc tggctttgtc
    20701 caaggagatg ccgttcgtcc acaaatgctc aaagaaagcg atgtggtcat cagtgacttg
    20761 cctgttggct attatcctga tgatgccgtt gcgtcgcgcc atcaagttgc ttctagccaa
    20821 gaacatactt acgcccatca cttgctcatg gaacaagggc ttaagtacct caagtcagac
    20881 ggatacgcta tttttctagc tccgagtgat ttgttgacca gtcctcaaag tgatttgtta
    20941 aaagaatggc tgaaagaaga ggcgagtctg gttgctatga ttagtctgcc tgaaaatctc
    21001 tttgctaatg ccaaacaatc taagactatt tttatcttac agaagaaaaa tgaaatagca
    21061 gtagagcctt ttgtttatcc acttgctagc ttgcaagatg caagtgtttt aatgaaattt
    21121 aaagaaaatt ttcaaaaatg gactcaaggt actgaaatat aaaatagatt ttgttataat
    21181 agttgaaaac gcttaaaaag gggtatcatg ttatgacaaa aacaattgca atcaatgcag
    21241 gaagttcaag tttgaaatgg caattatact taatgccaga agaaaaagta ttggcgaaag
    21301 gtttgattga acgtatcggt ttgaaagatt caatttcaac tgtaaaattt gacggccgtt
    21361 ctgaacaaca aattttggat attgaaaatc atacacaagc cgttaaaatt ttattggatg
    21421 acttgattcg tttcgatatt atcaaggctt atgacgagat tacaggtgtt ggacatcgtg
    21481 ttgttgctgg tggagaatat ttcaaagaat caacagttgt tgagggagat gttttagaaa
    21541 aagttgaaga gttgagtttg ttggctcctc tacacaaccc ggccaatgca gcaggtgttc
    21601 gtgccttcaa ggaattgttg ccagacatta ccagtgtagt tgtttttgat acttccttcc
    21661 acacaagtat gccagagaaa gcttatcgct accctctacc aacaaaatat tacacagaaa
    21721 acaaggttcg taaatacggt gctcatggta caagtcacca gtttgtagca ggagaagctg
    21781 caaaactctt gggacgtcca ttagaagact tgaagttaat tacctgtcat attggtaacg
    21841 gaggctcaat tacagctgtg aaagccggca aatctgtaga cacttctatg gggttcactc
    21901 ctcttggtgg tattatgatg ggaacgcgta caggggatat tgatccagct atcattcctt
    21961 atttaatgca atatacagag gattttaaca caccagaaga tatcagtcgt gttcttaacc
    22021 gtgaatcagg tcttttggga gtttctgcta attctagcga tatgcgcgat atagaagcag
    22081 ctgtagcaga agggaatcac gaggctagct tggcttatga aatgtatgtt gaccgtatcc
    22141 aaaaacatat cggtcagtac cttgcagtgc taaatggagc agatgccatt gttttcacag
    22201 caggtgtcgg tgaaaatgca gagagtttcc gtcgtgatgt aatctcaggg atttcgtggt
    22261 ttggttgtga tgttgatgat gaaaagaatg tctttggcgt tacaggagac atctcaacag
    22321 aggcagctaa aatccgtgtc ttggttattc caacagatga agaattagtc attgcccgtg
    22381 acgttgaacg cttgaaaaaa taagtgaaac taaaaaaata ttcaatacaa ggagttggga
    22441 aagttatttt tccagcttct ttttctgatg aaattgtcca aaaccttgct atgattggct
    22501 tttttgaaaa atatggtata atagtagtaa tttaatagat ggagttgagt tttgaagaaa
    22561 aactttcgtg taaaaagaga gaaagatttt aaggcgattt tcaaggaggg gacaagtttt
    22621 gctaatcgca aatttgtggt ctaccaatta gaaaaccaga aaaaccattt tcgagtaggt
    22681 ctatcagtta gcaaaaaact ggggaatgcc gtcactagaa atcaaattaa gcgacggatt
    22741 cggcatatta tccagaatgc aaaagggagt ctggtagaag atgtcgactt tgttgtcatt
    22801 gctcgaaaag gagtcgaaac cttgggatac gcagagatgg agaaaaatct actccatgta
    22861 ttaaaattat caaagattta ccgggaagga aatgggagtg aaaaagaaac taaagttgac
    22921 tagtttgcta ggactgtctc tgttaatcat gacagcctgt gcgactaatg gggtaactag
    22981 cgatattaca gccgaatcgg ctgatttttg gagtaaattg gtttacttct ttgcggaaat
    23041 cattcgcttt ttatcgtttg atattagtat cggagtgggg attattctct ttacggtctt
    23101 gattcgtaca gtcctcttgc cagtctttca ggtgcaaatg gtggcttcta ggaaaatgca
    23161 ggaagctcag ccacgcatta aggcgcttcg agaacaatat ccaggtcgag atatggaaag
    23221 cagaaccaaa ctagagcagg aaatgcgtaa agtatttaaa gaaatgggtg tcagacagtc
    23281 agactctctt tggccgattt tgattcagat gccggttatt ttggccttgt tccaagccct
    23341 atcaagagtt gactttttaa agacaggtca tttcttatgg attaaccttg gtagtgtgga
    23401 tacaaccctt gttcttccga ttttagcagc agtattcacc tttttaagta cttggttgtc
    23461 caacaaagct ttgtctgagc gaaatggcgc tacgactgcg atgatgtatg ggattccagt
    23521 cttgattttt atctttgcag tttatgcgcc aggtggagtc gccctatact ggacagtgtc
    23581 taatgcttat caagtcttgc aaacctattt cttgaataat ccattcaaga ttatcgcaga
    23641 gcgcgaggcc gtagtacagg cacaaaaaga tttggaaaat agaaaaagaa aagccaagaa
    23701 aaaggctcag aaaacgaaat aaataaggag gaatctggta atggtagtat ttacaggttc
    23761 aactgttgaa gaagcaatcc agaaaggatt gaaagaatta gatattccaa gaatgaaggc
    23821 tcatatcaaa gtcatttcta gggagaaaaa aggctttctt ggtctatttg gtaaaaaacc
    23881 agcccaagtg gatattgaag cgattagtga aacgactgtt gtcaaagcaa atcaacaggt
    23941 agtaaaaggc gttccgaaaa aaatcaatga tttgaacgag cctgtgaaga cggttagtga
    24001 agaaaccgtt gaccttggtc atgtggttga tgctattaaa aaaatagagg aagaaggtca
    24061 aggtatttct gatgaagtca aggctgaaat cttaaaacat gaaagacatg ccagcactat
    24121 cttagaagaa actggtcaca ttgagatttt aaatgaactt caaatcgagg aagcgatgag
    24181 ggaagaagca ggcgctgatg accttgaaac tgagcaagat caaactgaaa gtcaagaact
    24241 agaagacttg ggcttgaaag ttgaaacgaa ctttgatatt gaacaagtag ctacggaagt
    24301 aatggcttat gttcaaacga ttattgatga catggatgtt gaggctacac tttcaaatga
    24361 ttataaccgt cgtagcatca atctacaaat tgacaccaac gaaccaggtc gtattatcgg
    24421 ctaccatggt aaagtcttga aggccttgca actgttggct caaaattatc tttacaaccg
    24481 ctattccaga accttctacg ttacaatcaa tgtcaatgat tatgtcgaac accgtgcaga
    24541 agtcttgcag acctatgcgc aaaaattggc gactcgtgtt ttggaagaag ggcgcaatca
    24601 taaaacagat ccaatgtcaa atagcgaacg caagattatc catcgtatta tttcacgtat
    24661 ggatggcgtg actagttact ctgaaggtga tgagccaaat cgctatgttg ttgtagatac
    24721 agaataagta aaatcaggtt tatcctgatt ttttgctagt tagaggaggt taaactgatg
    24781 ttgaataaga taagagacta tttagacttt gctggtttgc agtaccgtaa tcctgataaa
    24841 gcgggagcag agcgagagaa gatgctggca ttccgccaca aaggacaaga ggctcgaaag
    24901 gcttttacag aactggccaa agcctttcaa gcaagccatc cagaatggca actccaacag
    24961 actagtcagt ggatgaatca ggcccagcgt ttgagaccac atttttgggt ttatctacag
    25021 agagacggac aagtgacaga acctatgata gccttgcgtt tgtatggaaa tccttctgat
    25081 tttggtattt ccttagaagt cagtttcatc gaacgtaaga aggatgagca aacactgggc
    25141 aagcaggcca aagttttaga aattccaacc gttaaaggga tttattatct aacctactct
    25201 aatggtcaaa gtcaacggtg ggaggcgaat gaagaaaagc gtcgtacttt acgcgagaag
    25261 gtgagaagtc aagaagttcg aaaagtttta gtgaaggtag atgttcctat gacagaaaat
    25321 tcgtctgaag aagaaatcgt agaaggctta ttgaagtctt attctaaaat tcttccctat
    25381 tatcaggtga ctagaggata acgtgtcagt caattattat aagattacag tccaagagtg
    25441 aacagtccgc tgtgtaattc ttggtctttt tgtttgcgct ttcgcattat ataataaact
    25501 tacaaaaaca attcaaaagg agaacaatta tggaagtcgt ttcaaatgtt ctaaattggt
    25561 tttctagcaa tattttgcag aatcccgcat ttttcgtagg tttattggtg ttgataggat
    25621 atgcactttt gaaaaaacct gcccatgacg ttttttcagg gtttgttaaa gcaacagtag
    25681 ggtatatgtt gcttaacgtg ggtgctggtg gtttggttac aacctttcgt ccaatcttag
    25741 cagctcttaa ctacaaattc caaattggtg cagcggttat cgacccttac tttggacttg
    25801 ctgcagcaaa caacaaaatt gcagcagagt ttccagattt tgttggaact gcaactacag
    25861 ctctattgat tggttttgga ataaatatct tgctcgtagc tcttcgaaag attacgaagg
    25921 taagaaccct ctttattact ggtcacatca tggtacaaca agctgcaaca gtatctctta
    25981 tggttctatt cttagtacca caattgcgca atgcttacgg tacagcagcg attggtatca
    26041 tctgtggact ttactgggca gttagttcaa atatgactgt tgaggcaact caacgcttga
    26101 ctggtggtgg cggatttgcg attggtcacc aacagcaatt tgcaatctgg tttgtagata
    26161 aagtagcagg acgctttggt aagaaagaag aaagtttaga caatcttaaa ttacctaagt
    26221 tcctctcaat cttccacgat acagttgttg catctgctac cttgatgctc gtattcttcg
    26281 gagccattct tttaatcttg ggtccagaca ttatgtctaa taaagaagtc atcacttcag
    26341 gaactctatt caatcctgct aaacaagatt tctttatgta cattatccaa acagccttta
    26401 ccttctcagt ttacttgttc gttttgatgc aaggtgtccg aatgttcgta tctgagttga
    26461 caaacgcctt ccaaggtatt tcaaacaaat tgttgccagg ttcattccca gcggttgacg
    26521 ttgcagcttc ttatggattt ggttctccaa atgctgtctt gtcaggattt acctttggtt
    26581 tgattggtca attgattata attgtcttgc tcatcgtctt taaaaatccg attcttatta
    26641 ttacaggatt tgtaccagtg ttctttgaca atgcagccat tgcggtctac gctgataaac
    26701 gcggcggatg gaaagcggct gttatccttt cctttatatc aggtgtcctt caagttgctc
    26761 taggagctct ttgtgtggcc cttctcgatt tggcagctta tggtggctac catggaaata
    26821 tcgactttga attcccatgg cttggatttg gatatatctt caaatacctt ggtattgttg
    26881 gttatgtact tgtgtgtctc ttcttgcttg ttattcctca acttcaattt gccaaagcaa
    26941 aagataaaga gaaatattac aacggtgaag ttcaagaaga agcttagtat ctagaaaagg
    27001 agaaataaaa tggttaaagt attagcagcg tgcggaaatg gaatgggttc atcaatggtt
    27061 atcaagatga aggttgaaaa tgctctccgt aagcttaatc aaagagattt tacagtcaat
    27121 tcatgcagtg tcggtgaagc taaaggttta gcagtaggat atgacatcgt aatcgcttct
    27181 cttcatttga ttcaagaatt ggaagggcga actaatggga agttaattgg gcttgataac
    27241 ttgatggatg ataaagaaat caccgaaaaa ctcagtcaag cactacagta aaaggttgga
    27301 gggggctgga cagaaactga gagttatcgt ttctgtcctt ctccctctta aataaaggag
    27361 gcagatatga atttaaaaca agctttaatt gacaatgact cgatccgact aggtttagag
    27421 gctaacaatt ggaaagaagc agtcaaggta gcagtagatc ccttaattga aagtggggca
    27481 attttgccag agtattacga tgctatcatt gaatcgactg aagagtatgg gccttactat
    27541 atcttgatgc caggtatggc tatgccccac gctagacctg aagctggtgt gcaaagtgat
    27601 gccttttcat tgattacctt acaaaatcct gttgtatttt cagatgggaa agaggtatct
    27661 gttttgttgg cactagcagc aacaagctca aaaattcaca caagtgtagc cattccacaa
    27721 attattgccc tgtttgaatt agaagattct attgcacgtt tacaggcttg ccagactaaa
    27781 gaagatgtct tggctatgat tgaagaatct aaggatagcc cttatctcga aggattggat
    27841 ttggaaagtt agaaagagga ataaagaaat gacaaaaaga atacctaatt tacaagttgc
    27901 attagaccat tcagacttgc aaggagcgat taaagcagct gtttctgttg gtcaggaagt
    27961 agatattatc gaagctggaa ctgtttgctt gcttcaagtt ggaagtgaac tggctgaagt
    28021 cttgcgtagc cttttcccag ataagattat tgtggcagac acaaaatgtg ctgatgctgg
    28081 tggaacagtt gctaaaaata atgcggttcg tggagcagac tggatgactt gtatctgttg
    28141 tgcaaccatc cctactatgg aagcagctct aaaggctatc aagactgaac gaggagaacg
    28201 aggcgaaatc cagatcgagc tttatggcga ttggactttt gaacaagctc agctttggct
    28261 agatgcaggt atctcacaag ctatttatca ccaatctcgt gatgctcttc ttgctggtga
    28321 aacttggggt gaaaaagacc ttaataaggt taaaaaactc attgacatgg gcttccgtgt
    28381 atctgtaaca ggtggtctag atgtagatac tctcaaactc tttgaaggtg ttgatgtctt
    28441 tacctttatc gcaggtcgtg gaattacaga ggctgcggat ccagcaggag cagcgcgtgc
    28501 cttcaaggat gaaatcaaac gaatttgggg gtaaatcatg gtacgtccaa ttggaattta
    28561 tgaaaaggca accccaacac actttacttg gctagaacgt ttaaattttg ccaaggagtt
    28621 aggctttgat tttgtcgaga tgtctattga cgaacgtgac gagcgtttag caagacttga
    28681 ctggagtaag gaagaacgct tggaagttgt caaagcaatc tatggaactg gtgttcgtat
    28741 tccttctatc tgtttttcag gccatcgtcg ctacccattg ggttcaaaag atccagttct
    28801 agaggaaaaa tctctagaac tcatgaaaaa atgtatcgaa ttagctcaag acttgggagt
    28861 tcgtacgatt caattagctg gttacgatgt ttactatgag gaaaagtcac cccagacacg
    28921 ccaacgtttt atcaaaaatt tgagaaaagc ctgtgactgg gctgaagaag ctcaggtggt
    28981 acttgctatt gaaattatgg atgatccttt catcaatagc atcgaaaaat atttggctat
    29041 agaaaaagag attgactctc ccttcctctt tgtatatcca gatattggta atgtgtctgc
    29101 atggcataat gatatctata gtgagtttta tcttggtcat catgccatcg cagctctcca
    29161 tctcaaggat acttatgcag tgacagaaag ttcaaggggc cagttccgag atgtaccttt
    29221 tgggcaaggt tgtgtcaaat gggaagaagc tttcgatatt ttaaaggaaa ccaattataa
    29281 tggacctttc ctaatcgaaa tgtggtctga aaattgtgaa acagtagaag aaacacgcgc
    29341 agccattcaa gaggcgcaag cttttctcta tccactcatt aagaaagcag gtttgatgta
    29401 agatgaatca agtaatcaat gctatgcgta aacgagtctg tgatgccaat caatcattgc
    29461 caaaacatgg acttgtcaaa tttacctggg ggaatgtatc tgaagtcaat cgcgaactcg
    29521 gtgtcattgt tatcaaacca tcaggcgtgg attatgacga attgacacct gaaaacatgg
    29581 tagtgactga tctagatggt aagatcctag aaggggattt aagaccatct tccgacctcc
    29641 caactcatgt gcaattatat aaggcttggt cagaaattgg tagtgtggtt cacacccatt
    29701 cgacagaagc tgttggttgg gctcaggcag gtcgtgatat tcctttctac ggaacaaccc
    29761 atgcagatta tttctacggt tcaatccctt gcgcccgtag tttgaccaag gacgaagtag
    29821 aagtggccta tgaaaaagat actggcctgg ttatcgtaga agaatttgaa catcgcggac
    29881 ttaacccggt tgaagtacca ggaattgttg tacgcaatca cggtccattc acctggggca
    29941 aaaatccaga gaatgctgtt tatcactctg tcgtactaga ggaagtatca aagatgaatc
    30001 gctttacaga acaaatcaat ccaagagttg gacctgctcc ccagtacata ctagaaaaac
    30061 actaccaacg taaacatgga ccaaatgctt attatggtca aaagtaagaa cgatgaagga
    30121 ggagaaaaag ataaatttag ctcctctttt tacatttgat ttttattgag agtaaagttg
    30181 gagttgaagt aattttgaaa gattttttag aaatagcgct tgatatatat atatatggta
    30241 aaataaaaag aattgctgtg atatcaatag atttggggga ttttttaata tggtactgga
    30301 taaggcaagt tgtgatttac ttcaatattt gatggatcaa gaaacgtcca aaacgattat
    30361 ggcgatttcg aaagatttga aagagtcaag aaggaaaatt tattatcaca ttgacaaaat
    30421 caatgctgct ctgggtgacg aggcgcttca catcattagt attccacgaa ttggtattca
    30481 cttaacggaa gagcagagag atgcttgttg taaactatta tcggaagtag attcgtacga
    30541 ttatatcatg agtgcgcatg aacgtatgat gataatgtta ttatggatag gtatttctaa
    30601 agaacgtatt acgattgaaa aattgataga gttaacagag gtatctagga atactgttct
    30661 caatgatttg aatagtattc gttatcaact aactttggaa caatatcagg tgaccttgca
    30721 agtgagcaag tcacagggat accaccttca tgcccaccct cttaataaaa ttcagtatct
    30781 tcaatcgctt ctatatcata tttttatgga agaaaatgcc acttttgtat ctattttaga
    30841 agataagatg aaagagaggt tagatgatga gtgtttgctt tctgttgaaa tgaaccaatt
    30901 ttttaaggaa caggttcctt tagttgaaca agatttaggg aagaaaataa accatcatga
    30961 aataactttt atgttgcagg ttctacctta tttgctgtta agctgtcata atgttgaaca
    31021 gtatcaagaa agacatcagg atatagagaa agaattttct ttgataagaa aaagaataga
    31081 gtatcaggtg tctaagaaat taggagaacg gttgtttcaa aagtttgaaa tttctttgtc
    31141 aggacttgaa gtttctcttg tagctgttct cctcctctcc tatcgtaaag atttggatat
    31201 tcatgcagaa agtgatgatt ttcggcaatt aaaacttgct ttagaagaat ttatctggta
    31261 ttttgaatca caaatccgaa tggagattga gaacaaggat gatttgttac gaaatttgat
    31321 gatccactgt aaagccttgt tatttagaaa gacttacggt attttttcta aaaatcctct
    31381 aacaaaacaa attcgatcca agtatggaga attattttta gtcactagaa aatctgcgga
    31441 aattttagaa ggagcatggt ttattcggct aacagacgat gatattgcct atttgacgat
    31501 tcatattgga ggatttttaa aatatacacc atcgtctcaa aaaaatatga aaaaagttta
    31561 tctcgtttgt gatgaaggtg ttgcggtttc gagacttttg ctgaaacaat gcaaacttta
    31621 ttttccaaat gagcaaattg acactgtatt tacaacagaa caatttaaga gtgtggaaga
    31681 tattgcacaa gttgatgtag tgattactac taatgatgat ttggatagca gatttccgat
    31741 tttaagggtt aatcctatcc ttgaagcaga agatattttg aaaatgctag actatcttaa
    31801 acacaatata tttcgtaata agagcaaaag tttcagtgaa aatctttcta gtcttatttc
    31861 gtcttatatt gtagacagca agttggctag taagttccaa gaagaggttc aaacacttat
    31921 aaatcaagaa atagtagttc aagctttttt ggaagatatt tgaaggacag tccaatgatg
    31981 aacacaaacc tgtgtttttc ttggtctttt ttagtgtttt gaagggtggt atactaatct
    32041 caaagataac aattatatcc aaaggaggca acatatgcca aacgtcaaag aaattacaag
    32101 agagtcatgg attttagcca ctttcccaga gtggggaaca tggttgaacg aagaaatcga
    32161 agaagaagtc gtacctgaag gcaactttgc catgtggtgg ctaggcaact gtggtacttg
    32221 gattaagaca ccagctggtg ctaacgttgt catggacctt tggtcaaacc gtggaaaatc
    32281 aaccaaaaaa gtgaaagata tggttcgtgg gcaccaaatg gcaaatatgg caggtgttcg
    32341 taagttgcaa ccaaacttgc gtgttcagcc aatggttatc gatccatttg ctatcaacga
    32401 actagactat tacttagttt cacacttcca cagtgatcat atcgacccat acacagctgc
    32461 agcaattctc aacaatccta agttagagca tgttaagttt atcggtcctt accactgtgg
    32521 acgaatctgg gaaggatggg gggttccaaa agaacgtatc atcgttgtta aaccaggtga
    32581 cactatcgaa ttaaaagata tgaagattca tgcagtagaa tcatttgacc gtacttgctt
    32641 ggtaactctc ccagtgaacg gtgctgatga gacaggcggt gaacttgctg gcttggctgt
    32701 tacagatgaa gaaatggctc aaaaggctgt taactatatc tttgaaacac caggtggaac
    32761 catctatcat ggtgcagatt ctcacttctc aaactatttt gcaaaacatg gtaaagactt
    32821 taaaattgat gttgctttga ataactatgg tgaaaatccg gtaggtatcc aagacaaaat
    32881 gacatctatc gaccttcttc gtatggcaga aaatctgcgt accaaagtca ttatcccagt
    32941 tcactatgat atctggtcta acttcatggc ttctactaat gagattctag aactttggaa
    33001 aatgcgaaaa gatcgcttgc aatacgattt ccatccattt atctgggaag ttggcggtaa
    33061 gtacacttat cctcaagatc aacacttagt agaataccat catccacgtg gttttgatga
    33121 ttgttttgaa caagactcta acattcaatt taaagctttg ctataatata aaaatattct
    33181 ttggaggtta tccgtatgtt gcatgatacg gataattttc atataatagt aaaatagaat
    33241 gtgtgattca ataatcacct caaatagaaa ggaaattcta tgtcaaatct atctgttaat
    33301 gcaattcgtt ttctaggtat tgacgccatt aataaagcca actcaggtca tccaggtgtg
    33361 gttatgggag cggctccgat ggcttacagc ctctttacaa aacaacttca tatcaatcca
    33421 gctcaaccaa attggattaa ccgcgaccgc tttattcttt cagcaggtca tggttcaatg
    33481 ctcctttatg ctcttcttca cctttctggt tttgaagatg tcagcatgga tgagattaag
    33541 agcttccgtc aatggggttc aaaaacacca ggtcacccag aatttggtca tacggcaggg
    33601 attgatgcta cgacaggtcc tctagggcaa gggatttcaa ctgctactgg ttttgcccaa
    33661 gcagaacgtt tcttggcagc caaatataac cgtgaaggct acaatatctt tgaccactat
    33721 acttacgtta tctgtggaga cggagacttg atggaaggtg tctcaagcga ggcagcttca
    33781 tacgcaggtt tgcaaaaact tgataagttg gttgttcttt atgattcaaa tgatatcaac
    33841 ttggatggtg agacaaagga ttcctttaca gaaagtgttc gtgaccgtta caatgcctac
    33901 ggttggcata ctgccttggt tgaaaatgga acagacttgg aagccatcca tgctgctatc
    33961 gaaacagcaa aagcttcagg caagccatct ttgattgaag tgaagacggt tattggatat
    34021 ggttctccaa acaaacaagg aactaatgct gtacacggcg cccctcttgg agcagatgaa
    34081 actgcatcaa ctcgccaagc cctcggttgg gactacgaac catttgaaat tccagaacaa
    34141 gtatatgctg atttcaaaga acatgttgca gaccgtggcg catcagctta tcaagcttgg
    34201 actaaattag ttgcagatta taaagaagct catccagaac tggctgcaga agtagaagcc
    34261 atcatcgacg gacgtgatcc agtcgaagtg actccagcag acttcccagc tttagaaaat
    34321 ggtttttctc aagcaactcg taattcaagt caagatgcct tgaatgttgt ggcagctaaa
    34381 ttaccaacct tcctaggtgg atcagctgac cttgctcact caaacatgac ttatatcaag
    34441 acggatggac ttcaagacga taccaatcgc ttgaatcgta acattcagtt tggtgttcgt
    34501 gaatttgcaa tgggaacaat cttgaacggg atggcccttc atggtggact tcgtgtatac
    34561 ggtggaactt tcttcgtctt ctctgactat gtgaaggcag ctgtccgctt gtcagcctta
    34621 caaggacttc ctgtgactta tgtctttacc catgattcaa tcgcagttgg ggaagatggt
    34681 ccgactcacg aaccagttga gcatttagca ggtcttcgtg ctatgccaaa tctaaatgtt
    34741 ttccgtccag cagatgcgcg tgaaacgcaa gcagcttggt accttgcagt gacaagtgag
    34801 aaaacaccaa ctgcccttgt cttgacacgt caaaacttga ctgttgaaga tggaacagac
    34861 ttcgacaagg ttgctaaagg tgcttatgtt gtatatgaaa atgcagccga ctttgatacc
    34921 atcttgattg cgacaggttc agaggttaat cttgctgtct cagctgctaa agaattggct
    34981 agtcaaggcg aaaaaatccg cgtagtcagc atgccatcta cagatgtctt tgataaacaa
    35041 gatgcagctt acaaggaaga aattcttcca aatgcagtcc gccgtcgtgt tgcagtcgaa
    35101 atgggtgcaa gtcaaaactg gtacaaatat gttggtctcg atggtgccgt tctaggtatt
    35161 gataccttcg gagcctctgc cccagcacca aaagtattgg cagaatatgg ctttactgtc
    35221 gaaaatcttg taaaagttgt tcgaaatttg aaataatcct aaaaatcagg gcgtaagctc
    35281 tggtttttct taccagaaaa gtaaggtaca atcttgtaaa agtagctgaa atttgatata
    35341 gtagtcctat gtaaaagaca aaggagaaaa caatgaatcc aaatattact tttttaatca
    35401 tgcttgtagg tatgatggcc ttgatgttct ttatgcaacg ttctcaaaag aaacaagctc
    35461 aaaagcgtat ggaaagctta aataaactac aaaaaggcta tgaagtgatt acaatcggtg
    35521 gactttatgg aacagtcgat gaagtagata cggagaaggg aacaatagtt cttgacgtag
    35581 atggagttta cttgactttt gaactagctg ctatcaagac agtattaccg ctgaaagaaa
    35641 cagcttcact agaaggcgca attgaaaaat aagacgggat cacgaactcc cgttttctat
    35701 aaaagaaagg aaatgggatg aaaaaattag tctttgtctg tctgggaaat atttgccgta
    35761 gccctatggc cgagtttgtt atgaaatcaa tgacagataa ctacgaaatc caaagtcgag
    35821 caacttcctc ttgggaacat ggcaatccga ttcataaggg gactcaggga atttttcaag
    35881 agtatgagat tccttatgac aagaacaaga catcgcttca gattagtaag gaagattttg
    35941 aagcctttga ttatattatc ggaatggacg cttcaaatat ttctgactta cgtcagatgt
    36001 gtccagtaga ctgtcaagat aagatttact cattttcatc tgaaagtgtt ccagaccctt
    36061 ggtatacagg agattttgaa gaaacctatc gacgtgttca agagggctgt caagcttggt
    36121 tagaacgttt agaaaaggag agttaaggtg gaaaatctta cgaattttta cgaaaagtat
    36181 cgtgtctatc tgactcgtcc acgtttagag cttttggcag tagttaccat tgttttctgt
    36241 gctgtactcg tcttttttct aaatattcca ggaaaaggtg tcttaaaact cgataatgga
    36301 acgattgttt atgatggcag tcttgtccgt ggtaaaatga atggccaagg taccattacc
    36361 ttccaaaatg gagaccaata tacaggtggc ttcaacaatg gagccttcaa cggaaaaggt
    36421 acctttcaat ctaaagaagg ctggacctac gaaggtgatt ttgtaaatgg tcaggctgaa
    36481 ggaaaaggga aactaacaac agaacaagaa gtcgtttatg aaggaacttt taaacaaggc
    36541 gtttttcaac aaa
//
LOCUS       DAVAAL010000008        35396 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVAAL010000008 DAVAAL010000000
VERSION     DAVAAL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35396
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.008"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            186..416
                     /gene="blpU"
                     /locus_tag="VQM60_000285"
     CDS             186..416
                     /gene="blpU"
                     /locus_tag="VQM60_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET1080186.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            1131..3284
                     /locus_tag="VQM60_000286"
     CDS             1131..3284
                     /locus_tag="VQM60_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET1080187.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            3297..4646
                     /locus_tag="VQM60_000287"
     CDS             3297..4646
                     /locus_tag="VQM60_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET1080188.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKVTASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKYVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4816..5523
                     /locus_tag="VQM60_000288"
     CDS             4816..5523
                     /locus_tag="VQM60_000288"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET1080189.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNVAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            5580..9305
                     /locus_tag="VQM60_000289"
     CDS             5580..9305
                     /locus_tag="VQM60_000289"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET1080190.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELKAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVVSDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9398..10840
                     /locus_tag="VQM60_000290"
     CDS             9398..10840
                     /locus_tag="VQM60_000290"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET1080191.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10877..11899
                     /locus_tag="VQM60_000291"
     CDS             10877..11899
                     /locus_tag="VQM60_000291"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET1080192.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGLHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11896..12441
                     /gene="purN"
                     /locus_tag="VQM60_000292"
     CDS             11896..12441
                     /gene="purN"
                     /locus_tag="VQM60_000292"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET1080193.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12525..13034
                     /locus_tag="VQM60_000293"
     CDS             12525..13034
                     /locus_tag="VQM60_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET1080194.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEILFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            13059..14606
                     /gene="purH"
                     /locus_tag="VQM60_000294"
     CDS             13059..14606
                     /gene="purH"
                     /locus_tag="VQM60_000294"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET1080195.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14728..15990
                     /gene="purD"
                     /locus_tag="VQM60_000295"
     CDS             14728..15990
                     /gene="purD"
                     /locus_tag="VQM60_000295"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET1080196.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTAIYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16393..16881
                     /gene="purE"
                     /locus_tag="VQM60_000296"
     CDS             16393..16881
                     /gene="purE"
                     /locus_tag="VQM60_000296"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET1080197.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16868..17959
                     /gene="purK"
                     /locus_tag="VQM60_000297"
     CDS             16868..17959
                     /gene="purK"
                     /locus_tag="VQM60_000297"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET1080198.1"
                     /translation="MSSSKTIGIIGGGQLGQMMATSAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17969..18196
                     /locus_tag="VQM60_000298"
     CDS             17969..18196
                     /locus_tag="VQM60_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET1080199.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            18259..19557
                     /gene="purB"
                     /locus_tag="VQM60_000299"
     CDS             18259..19557
                     /gene="purB"
                     /locus_tag="VQM60_000299"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET1080200.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19612..23550)
                     /gene="strH"
                     /locus_tag="VQM60_000300"
     CDS             complement(19612..23550)
                     /gene="strH"
                     /locus_tag="VQM60_000300"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET1080201.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSHLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(23865..24581)
                     /locus_tag="VQM60_000301"
     CDS             complement(23865..24581)
                     /locus_tag="VQM60_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET1080202.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFISRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            24929..26716
                     /locus_tag="VQM60_000302"
     CDS             24929..26716
                     /locus_tag="VQM60_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET1080203.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26713..27189
                     /locus_tag="VQM60_000303"
     CDS             26713..27189
                     /locus_tag="VQM60_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET1080204.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            27217..28122
                     /locus_tag="VQM60_000304"
     CDS             27217..28122
                     /locus_tag="VQM60_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET1080205.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            28109..28924
                     /locus_tag="VQM60_000305"
     CDS             28109..28924
                     /locus_tag="VQM60_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET1080206.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28931..29335
                     /locus_tag="VQM60_000306"
     CDS             28931..29335
                     /locus_tag="VQM60_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET1080207.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            29629..30795
                     /locus_tag="VQM60_000307"
     CDS             29629..30795
                     /locus_tag="VQM60_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET1080208.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            30911..31948
                     /locus_tag="VQM60_000308"
     CDS             30911..31948
                     /locus_tag="VQM60_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET1080209.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYET
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            32155..33009
                     /locus_tag="VQM60_000309"
     CDS             32155..33009
                     /locus_tag="VQM60_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET1080210.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            33159..33416
                     /locus_tag="VQM60_000310"
     CDS             33159..33416
                     /locus_tag="VQM60_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET1080211.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(33629..33712)
                     /locus_tag="VQM60_000311"
                     /pseudo
     CDS             complement(33629..33712)
                     /locus_tag="VQM60_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
     gene            complement(33746..33838)
                     /locus_tag="VQM60_000312"
                     /pseudo
     CDS             complement(33746..33838)
                     /locus_tag="VQM60_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(33874..34170)
                     /locus_tag="VQM60_000313"
     CDS             complement(33874..34170)
                     /locus_tag="VQM60_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET1080212.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(34160..34321)
                     /locus_tag="VQM60_000314"
     CDS             complement(34160..34321)
                     /locus_tag="VQM60_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HET1080213.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            34391..35155
                     /locus_tag="VQM60_000315"
     CDS             34391..35155
                     /locus_tag="VQM60_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET1080214.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
BASE COUNT         9765 a         6867 c         8325 g        10439 t
ORIGIN      
        1 tagtaaaaaa gtgattagaa aacatctttt ttaaaaatag agatgatttt gaaacaaaaa
       61 agctaattca agacgtttcg atgccaattc aagatttgga tgaaaaaaat taatagatac
      121 tgttatacta aacttgtcaa gtttgtaaca agacaaatat taaaaataaa aaagaggtat
      181 tcgttatgaa tacaaaaaag atgtcacaat ttgaaattat ggatactgag atgcttgctt
      241 gcgttgaagg tggcggatgc aattggggag attttgccaa agcaggtgtt ggaggaggag
      301 cagcacgagg tcttcagcta ggaattaaaa caagaacatg gcaaggtgca gcaactggtg
      361 ctgcgggagg agctatactt ggaggtgtgg cctatgcagc gacatgttgg tggtaattat
      421 ggattttaaa agttttatta ttggtttagt agttggtata tttggtcctt atatggatga
      481 tttaattaga aaaaaatttt taaagtcttc ggagaagaaa acagaaaaat ctgttaaaaa
      541 ataatcaaaa ctataaatga tgaatctgaa tcaaaattat tttgcgcatg taaagaggag
      601 tcttatagta acgagtcaaa aaaggagtaa ctatgaatcg taatttagaa cggtgttatc
      661 tattctgact aggaatagat cataccagag gtagcttaga aatagcagag acattagaaa
      721 ttgaagtaat aaataggatg tcgtaagtgt tactatcaat gatttatttg tttcaagctt
      781 gcctagggtg acagtaaaaa atcaatttcc tttcaatagc atatttttag tgggcaggac
      841 tcttgttctg cctatttttt tatccaaaaa gtgcagttgg gagggagata ggcgcatttg
      901 ggaaggaagt ccagtttttg tttagtgatt ggggtaagat agttgttatc agatgagttt
      961 atactcttcg aaaatcaaat tcaaaccacg tcaacgtcgc cttgccgtat atatgtgact
     1021 gacttcgtca gtcctatcta caacctcaaa acagtgtttt gagcagcctg cggctagttt
     1081 cctagtttgc tctttgattt tcattgagta ttagggaaaa ggagatgaat atgaaatttg
     1141 ggaaacgtca ctatcgtccg caagtggatc agatggactg cggtgtagct tcattagcca
     1201 tggtttttgg ctactatggt agttattatt ttttggctca cttgcgagaa ttggctaaga
     1261 cgaccatgga tgggacgacg gctttgggct tggtcaaggt ggcagaggag attggttttg
     1321 agacgcgagc cattaaggcg gatatgacgc tttttgactt gccggatttg acttttcctt
     1381 ttgttgccca tgtgcttaag gaagggaaat tgctccacta ctatgtggtg actgggcagg
     1441 ataaggatag cattcatatt gccgatccag atcccggggt gaagttgact aaactgccac
     1501 gtgagcgttt tgaggaagaa tggacaggag tgactctttt tatggcacct agtccagact
     1561 ataagcctca taaggaacaa aaaaatggtc tgctctcttt tatccctata ttagtgaagc
     1621 agcgtggctt gattgccaat atcgttttgg caacactctt ggtaaccgtg attaacattg
     1681 tgggttctta ttatctgcag tctatcattg atacctatgt gccagatcag atgcgttcga
     1741 cactagggat tatttctatt gggctagtca tcgtctacat cctccagcaa atcttgtctt
     1801 acgctcagga gtatctcttg cttgttttgg ggcaacgctt gtcgattgac gtgattttgt
     1861 cctatatcaa gcatgttttt cacctcccta tgtccttctt tgcgacacgc aggacagggg
     1921 agatcgtgtc tcgttttaca gatgctaaca gtatcatcga tgcgctggct tcgaccatcc
     1981 tttcgatttt cctagatgtg tcaacggttg tcattatttc ccttgttcta ttttcacaaa
     2041 ataccaatct ctttttcatg actttattgg cgcttcctat ctacacagtg attatctttg
     2101 cctttatgaa gccgtttgaa aagatgaatc gggataccat ggaagccaat gcggttctgt
     2161 cttcttctat cattgaggac atcaacggta ttgagactat caagtccttg accagtgaaa
     2221 gtcagcgtta ccaaaaaatt gacaaggaat ttgtggatta tctgaagaaa tcctttacct
     2281 atagtcgagc agagagtcag caaaaggctc tgaaaaaggt tgcccatctc ttgcttaatg
     2341 tcggcattct ctggatgggg gctgttctgg tcatggatgg caagatgagt ttggggcagt
     2401 tgattaccta taataccttg ctggtttact ttaccaatcc tttggaaaat atcatcaatc
     2461 tgcaaaccaa gcttcagaca gcgcaggttg ccaataaccg tctaaatgaa gtgtatctag
     2521 tagcttctga gtttgaggag aagaaaacag ttgaggattt gagcttgatg aagggagata
     2581 tgaccttcaa gcaggttcat tacaagtatg gctatggtcg agacgtcttg tcggatatca
     2641 atttaaccgt tccccaaggg tctaaggtgg cttttgtggg gatttcaggg tcaggtaaga
     2701 cgactttggc caagatgatg gttaattttt acgacccaag tcaaggggag attagtctgg
     2761 gtggtgtcaa tctcaatcag attgataaaa aagccctgcg ccagtacatc aactatctgc
     2821 ctcaacagcc ctatgtcttt aacggaacga ttttggagaa tcttcttttg ggagccaagg
     2881 aggggacgac acaggaagat atcttacggg cggtcgaatt ggcagagatt cgagaggata
     2941 tcgagcgcat gccactgaat taccagacag aattgacttc ggatggggca gggatttcag
     3001 gtggtcaacg tcagagaatc gctttggcgc gtgctctctt gacagatgcg ccggtcttga
     3061 ttttggatga ggcgactagc agtttggata ttttgacaga gaagcggatt gtcgataatc
     3121 tcatggcttt ggacaagacc ttgattttca ttgctcaccg cttgactatt gctgagcgga
     3181 cagagaaggt agttgtcttg gatcagggca agattgtcga agaaggaaag catgctgatt
     3241 tgcttgcaca gggtggcttt tacgcccatt tggtcaatag ctagaaagag gagaggatga
     3301 aaccagaatt tttagaaagt gcggagtttt ataatcgtcg ttaccataat ttttccagta
     3361 gtgtgattgt acccatggcc cttctgcttg tgtttttact tggctttgca actgttgcag
     3421 agaaggagat gagtttgtcc actagagcta ctgtcgaacc tagtcgtatc cttgcaaata
     3481 tccagtcaac tagcaacaat cgtattcttg tcaatcattt ggaagaaaat aagctggtta
     3541 agaaggggga tcttttggtt caataccaag aaggggcaga gggtgtccaa gcggagtcct
     3601 atgccagtca gttggacatg ctaaaggatc aaaaaaagca attggagtat ctgcaaaaga
     3661 gcctgcaaga aggggagaac cactttccag aggaggataa gtttggctac caagccacct
     3721 ttcgcgacta catcagtcaa gcaggcagtc ttagggctag tacatcgcaa caaaatgaga
     3781 ccatcgcgtc ccagaatgca gcagctagcc aaacccaagc cgaaatcggc aacctcatca
     3841 gtcaaacaga ggctaaaatt cgcgattacc agacagctaa gtcagctatt gaaacaggtg
     3901 cttccttggc cggtcagaat ctagcctact ctctttacca gtcctacaag tctcagggcg
     3961 aggaaaatcc ccaaactaag gttcaggcag ttgcacaggt tgaagcacag atttctcagt
     4021 tagaatctag tcttgctact taccgtgtcc agtatgcagg ttcaggtacc cagcaagcct
     4081 atgcgtcagg gttaagcagt caattggaat cccttaaatc ccaacacttg gcaaaggttg
     4141 gtcaggaatt gacccttcta gcccagaaaa ttttggaggc agagtcaggt aagaaggtac
     4201 agggaaatct tttagacaag gggaaagtta cggcgagtga ggatggggtg cttcatctta
     4261 atcctgagac cagtgattct agcatggttg cagaaggtgc cctactagcc caactttatc
     4321 catctttgga aagagaaggg aaagccaaac tcacagctta tctaagttca aaatatgtag
     4381 caagaatcaa ggtcggtgat tctgttcgct atactacgac tcatgatgcc gggaatcaac
     4441 ttttcctaga ttctactatt acaagtattg atgcgacagc tactaagact gagaaaggga
     4501 atttctttaa aatcgaggcg gagactaatc taacttcgga gcaggctgaa aaacttaggt
     4561 acggggtgga aggccgcttg cagatgatta cgggcaagaa aagttaccta cgttattatt
     4621 tggatcaatt tttgaacaaa gagtaatgtt cgtgttttta gagttaaata atttttaaac
     4681 tgtgagaaag attcttcttg cagttttttc tttacaattt ttgaaaaaca tctactattt
     4741 attcggttaa attcttgtgt tttttggttt tttgtggtaa aatgtgctca agtaatacga
     4801 aaggcgaact ttaaaatgtc aaaacaattg atctattcgg gaaaagctaa agatatctat
     4861 acaactgagg atgaaaatct tattatttca acttacaagg accaggcgac tgctttcaac
     4921 ggtgtcaaga aggagcagat tgcaggtaag ggagtcttga ataatcagat ctcatctttt
     4981 atttttgaga aattaaatgt ggctggtgtg gcgactcact ttgtggagaa actttcagac
     5041 acggaacaac tcaataaaaa ggttaagatt attcctttgg aagtcgtgct ccgcaactat
     5101 actgctggtt ccttttcaaa acgttttggt gtggatgagg gaatcgcctt ggagactccg
     5161 attgtcgaat tttactacaa aaatgatgat ttggatgatc catttatcaa tgatgagcat
     5221 gtgaaattcc tacagattgc gggtgaccag cagattgcct acttgaagga agaaacgcgt
     5281 cgtatcaatg aactattgaa agtctggttt gctgagattg ggcttaaatt gattgacttt
     5341 aagctagagt tcggttttga caaggatggc aagattatct tggcagacga attttcacca
     5401 gataactgcc gcttgtggga cgctgatggc aaccacatgg ataaggatgt tttccgtaga
     5461 ggattgggag aactaaccga cgtttatgag attgtttggg aaaagttgca gggtttaaaa
     5521 taacaacctc aaggctgttt gggaatattg caagagctga aataaaggaa taagaattga
     5581 tggataaacg tatttttgtt gaaaaaaagg ctgattttca ggtcaagtca gagagtttgg
     5641 ttagagagct ccagcacaac ttgggactgt caagcttgaa aagtattcgt attgtgcaag
     5701 tatatgatgt atttgacttg gttgaggact tgtttgcacc tgcagagaag cacattttct
     5761 ctgagcaggt aaccgaccat gttttagatg aagtatctgt gcaggcggat cttgctaact
     5821 atgctttctt tgccattgaa agtctaccag ggcagtttga ccagcgtgca gcttcgtcac
     5881 aggaagcctt gcttttgttg ggaagttcga gtgacgtgac agtcaacaca gcccaacttt
     5941 acttggtgaa taaagatatt gatgcgactg agttgaaagc tgtcaaaaac tacctgctca
     6001 atccagttga ttctcgtttc aaggatatca cgacagggat tgccaagcag gagttttcag
     6061 agtcagacaa gaccattcca aaattgactt tctttgaaag ctatacagca gaagattttg
     6121 ctcgctacaa ggccgaacaa gggatggcca tggaagtgga tgatttgctc tttatccaag
     6181 actactttaa gtcaatcggg cgcgtgccaa ctgagactga acttaaggtt ttggacactt
     6241 actggtctga ccactgccgt catacgactt ttgagacaga gttgaaacac atcgactttt
     6301 cagcttctaa atttcaaaag caattgcagt caacctatga caagtatatt gccatgcgcg
     6361 aggaattagg tcggtctgaa aaaccacaaa ccttgatgga tatggcgact attttcggtc
     6421 gttatgagcg tgctaatgga cgattggatg atatggaagt ctctgacgaa atcaatgcct
     6481 gctcagttga aattgaagtg gacgttgatg gtgtcaagga accttggctc ctcatgttta
     6541 aaaacgaaac ccacaaccat ccaacagaaa ttgagccatt tggtggagcg gctacctgta
     6601 ttggtggagc tattcgtgat ccgttgtcag gccgttccta tgtttaccaa gccatgcgta
     6661 tttcaggtgc tggtgatatt acagcaccga tttcggaaac tcgcgctggg aaattgccac
     6721 aacaagtcat ttctaaaaca gcagctcatg gttattcttc atatggtaac cagattgggc
     6781 ttgccacaac ctacgttcgt gaatacttcc acccaggctt tgtagctaaa cgtatggaac
     6841 ttggtgccgt tgttggtgcg gctcccaagg gcaatgttgt ccgtgaaaaa cctgaagcag
     6901 gtgatgtgat catccttctc ggaggtaaaa caggtcgtga tggtgtcggt ggtgcgacgg
     6961 gctcttctaa ggttcaaaca gttgagtctg tagagactgc tggtgctgag gttcaaaaag
     7021 gaaatgccat cgaagaacgc aagattcagc gcctcttccg taatggcaat gtcactcgtc
     7081 tgatcaagaa gtccaatgac tttggggcag gcggtgtctg tgtggctatc ggtgaattgg
     7141 cagacggtct tgaaatcgac ctcaacaagg tgcctcttaa ataccagggc ttgaatggta
     7201 cagaaattgc catctctgaa tcacaagaac ggatggcggt cgtggttcgt cctgaagatg
     7261 tggatacctt cgttgccgaa tgtaacaaag aaaatattga tgctgttgtg gtggcgacag
     7321 taactgaaaa accaaatctt gtcatgcact ggaatggtga gacaatcgtt gacttggagc
     7381 gtcgtttcct tgacaccaat ggtgtgcgcg tggttgtcga tgccaaagtt gtggacaagg
     7441 atgtcaaact cccagaagag cgtcaaacat ctgctgaaac actggaatca gataccctta
     7501 cggttctatc tgacctcaac catgcaagtc aaaaaggatt acagactatc tttgactgct
     7561 ctgttggacg ctcaacggtt aatcacccac ttggtggtcg ttaccaactc acaccaactg
     7621 aggcatctgt gcagaaattg ccagttcaac acggtgtgac tcatactgcg tcggtcattg
     7681 ctcaaggttt caacccatat gtagctgaat ggtctccata ccacggtgct gcttatgcgg
     7741 ttatcgaagc aactgctcgt ttggtggctg ctggtgccaa ctggtctaag gctcgtttct
     7801 cttaccaaga atatttcgag cggatggaca agcaagcaga gcgttttggt cagccagtag
     7861 ccgcccttct aggctctatt gaagcacaaa ttcagcttgg cttgccatct atcggtggta
     7921 aggactccat gtctggtacc tttgaagaat tgaccgttcc gccaaccttg gttgcctttg
     7981 gggtgacgac ggcagatagc cgtaaggtgc tctctccaga atttaaagct gttggggaaa
     8041 atatctacta catcccaggt caagccctct ctgcagagat tgattttgac ttgattaaga
     8101 aaaattttgc tcagtttgaa gcaagccaag ctgaccataa agtgacatct gcatcagctg
     8161 tcaaatacgg tggtgtagtt gaaagtttgg ctcttgctac ctttggaaac tatattggtg
     8221 cagaggtgac cttgcctgaa cttaaaacag ctttgacagc tcaattaggc ggctttgtct
     8281 tcacatctcc tgaagaaatt gctggagtag agaagattgg acaaacgaaa gcagacttta
     8341 cactgactgt caacggtgtg aagctagatg gacacaagct tgacagtgca tttcaaggga
     8401 cattggaaga agtttaccca acagaattta cccaagcgaa agaactagaa gaagtaccag
     8461 ctgtggtatc agatgttgtg attaaagcca aagaaaaggt tgaaaaacct gtggtttaca
     8521 tcccagtctt tccaggaacc aactcagaat atgattcagc taaggccttc gaaaaagaag
     8581 gtgcagaggt caatttggtg ccattcgtga ccttgaatga agaagctatt gtcaagtcag
     8641 ttgaaactat ggttgacaat atcgacaaga ctaatattct cttctttgcc ggtggattct
     8701 cggctgcgga tgaaccagat ggttcagcta agtttatcgt caatatcctg cttaatgaaa
     8761 aagtgcgtgt ggctattgat agctttatcg cccgtggtgg cttgattatc ggtatttgta
     8821 atggattcca agccttagtc aaatcgggtc tcctaccata cggaaacttt gaagatgcta
     8881 acagtactag tccaaccctc ttctacaatg atgccaacca acacgtggcc aagatggtgg
     8941 aaactcgtat tgccaatacc aactcaccat ggttggctgg tgtgcaagtg ggcgatatcc
     9001 acgctattcc tgtttcgcac ggtgaaggga agtttgtcgt gacggctgag gaatttgcag
     9061 agctccgtga caatggacaa attttcagcc aatacgttga ctttaacggt aaaccaagta
     9121 tggattctaa gtacaatccg aatggttctg tccatgccat cgaaggaatt accagcaaga
     9181 atggtcaaat catcggtaag atgggccact cagaacgtta tgaggatggt cttttccaaa
     9241 atatcccagg caataaagac caacacctgt tcgcatcagc ggttaaacat ttcactggaa
     9301 aataagactt acagattttc taatagatag tatcagtaat gtaaaagtca tgtaaatcta
     9361 gctcttgatg attacaaatg aaaattaggt ataaaaaatg acatacgaag taaaatctct
     9421 taatgaagaa tgtggtgttt tcggtatttg gggacatcca gatgctgcta agttgaccta
     9481 ttttggactc cacagtcttc aacaccgtgg tcaggagggg gcaggaatcc tctccaatga
     9541 tcaaggacaa ctgaagcgcc atcgtgacat ggggctttta tcagaagttt tcagaaatcc
     9601 agccaatttg gataaattga caggagctgg tgcgattggg catgtgcgtt atgcgactgc
     9661 tggcgaagct tctgttgata acatccagcc cttcctcttc cgttttcacg atatgcagtt
     9721 tggcttggct cataatggaa atctgaccaa tgcagcctct ctcaagaaag aactggaaca
     9781 aagaggagca attttcagcg cgacttcgga ctctgaaatc ttggctcacc tcattcgtcg
     9841 cagtcataat cctagcctga tgggtaaaat caaggaagcg ctcagccttg tcaaaggtgg
     9901 ttttgcctat atcttgctgt ttgaggacaa gttgattgcg gctcttgacc ctaatggctt
     9961 tcgtccgctt tctatcggga aaatggccaa cggagcggtg gttgtttcct ctgaaacctg
    10021 tgcttttgag gtcattggtg ccgagtggat tcgtgatttg aagccaggtg agattgtgat
    10081 cattgatgac gagggcattc agtatgacag ctatacagat gatactcagt tggcgatttg
    10141 ttctatggag tatatctact ttgctcgccc tgattctaat atccacggtg tcaatgtcca
    10201 tacggcacgt aagagaatgg gagcgcaatt ggcgcgagaa tttaagcatg aggcagatat
    10261 tgtagttggt gtgcccaatt cttccctaag cgcggctatg ggatttgcgg aagaatcagg
    10321 cttaccaaat gaaatgggtc tgatcaaaaa ccaatacacc cagcgaactt ttatccaacc
    10381 gactcaagaa ttgcgggagc aaggagtgcg gatgaaactg tctgctgttt cgggtgttgt
    10441 caaaggcaaa cgtgtggtca tggtggatga ttccattgta cgtggaacaa cctctcgtcg
    10501 tatcgttcag ctcttgaaag aagcgggtgc gactgaggtt cacgttgcca ttggaagtcc
    10561 tgcactagcg tatccatgtt tctacgggat tgatatccag acccgtcagg agctgattgc
    10621 agccaatcat acggtcgaag aaactcgcca aatcattggt gcggacagtc tgacttatct
    10681 ttcaattgat ggcttgattg agtcgattgg tatcgaaaca gatgcgccga acggtggtct
    10741 ctgtgtcgct tactttgacg gtgactaccc aacgcctctc tatgactacg aagaagacta
    10801 tcgtagaagt ttggaagaaa agaccagttt ttacaagtag gcgacagatt ctccattaaa
    10861 gaaaaggaaa aaacaaatga caaataaaaa tgcatatgcc cagtcgggtg tggatgttga
    10921 agcgggttat gaagttgttg aacggattaa aaagcacgtg gcccgtacgg agcgtgcagg
    10981 tgtcatggga gctcttggtg gctttggtgg tatgtttgac ctttccaaga ctggggttaa
    11041 agaacccgtc ttgatttcag ggactgacgg tgtcggaacc aagctcatgt tggctatcaa
    11101 gtacgacaag cacgatacca tcgggcagga ctgtgtggcc atgtgtgtca acgacatcat
    11161 tgctgcaggt gcggaacccc tctattttct cgactacgta gcgacaggga agaatgagcc
    11221 agctaagcta gaacaagtgg ttgctggtgt ggcagaaggt tgtgtgcagg ctggtgctgc
    11281 cctcatcggt ggggaaacgg ctgaaatgcc gggcatgtac ggcgaagacg actatgactt
    11341 ggctggtttt gcggtcggtg tggctgaaaa atctcaaatc attgacggtt caaaggtggt
    11401 agagggagac gttcttctcg gacttgcttc aagtgggctt cactcaaatg gttactcttt
    11461 ggttcgtcgt gtctttgcgg attacacagg tgaggaagtc ctaccagaat tggaaggcaa
    11521 gaaacttaag gaagttctac ttgagccgac tcgtatctat gtcaaggctg tcttgccgct
    11581 catcaaagaa gagttggtca acggcattgc ccacatcaca ggtggtggct ttatcgaaaa
    11641 tgtccctcgt atgtttgcag atgacctagc tgctgaaatt gatgaaagca aagttccagt
    11701 gcttccaatt ttcaaagccc ttgaaaaata tggtcagatt aaacacgaag aaatgtttga
    11761 aatcttcaat atgggtgtgg gacttatgtt ggcggtcagc cctgaaaatg tagagcgtgt
    11821 aaaagaattg ttggatgaag cagtctatga aattggtcgc atcgtcaaga aagaaaacga
    11881 aagtgtcatt atcaaatgaa aaaaatagcg gtttttgcct ctggtaatgg ctcaaatttt
    11941 caggtgattg ccgaagaatt tccagtggag tttgtctttt cagaccatcg tgatgcctat
    12001 gtgcttgagc gtgcaaagca gctcggcgtt ctgtcctatg cttttgaact caaggagttt
    12061 gagagcaagg cagactacga agcagctctt gtcgaactct tggaagaaca ccagattgac
    12121 ttggtttgcc tagcaggcta catgaaaatc gttggaccaa ccttattgtc ggcttatgaa
    12181 ggtcggattg tcaacattca tccagcctac ttgccagaat ttccaggagc tcatgggatt
    12241 gaggatgctt ggaatgctgg cgtgggtcag tctggtgtga ccattcactg ggtggattcg
    12301 ggtgtggata caggccaggt catcaaacag gttcgtgtgc cacgactagc tgatgatacc
    12361 attgacagat ttgaagctcg catccatgaa gcagagtaca ggttgtatcc ggaagtagtg
    12421 aaggctctat ttacagattg actttttgat gattcatatg atatctttga ttttaaattg
    12481 gagtcagtgt ttgttgaaga cggcttcaaa cggaggtatt tgtaatgtta gaatctaaaa
    12541 aaacaactcg atatgtattt tatgtctatc tgatgttatt aacttgggga atcttattta
    12601 agtttgaaac aaatcctgaa tttatagcat ttttcttagc tccaaggtat atcaattgga
    12661 ttccattttc agaaccacta atagtcgatg gaaaaattgt ttttgctgaa atattattta
    12721 atctgatttc ctttattcca ttaggtgttt gtttcccttt gataaaaact aatttatcta
    12781 gtttaagaat agtcgggaca ggtttcttga ttagtttatt gtttgagtgc ttacagtata
    12841 ttttagcaat aggtataaca gatataacgg atttgacttt aaatacgcta ggtgtctgtg
    12901 taggcttact gatttatcaa atttttataa gagtgttcaa atcacagact agaaaatgga
    12961 tcaatatctt aggtatgctt agccttggtt ttgcttatct tgttttactg ttactgcatt
    13021 taattggtgt ttaactaatg attaaaaagg agaatataat gactaaacgc gtcttaatca
    13081 gcgtctcaga caaagcgggc attgttgaat ttgcccaaga actcaaaaaa cttggttggg
    13141 agattatctc aacaggtgga actaaggttg cccttgataa tgctggggtg gataccattg
    13201 ctatcgatga tgtgactggt ttcccagaaa tgatggacgg tcgtgtgaag accctccacc
    13261 caaatatcca cggagggctt ctcgctcgtc gtgacttgga tagccacttg gaagcggcta
    13321 aggacaacaa gattgagctc attgaccttg tggtggtcaa cctttaccca tttaaggaaa
    13381 ctatccttaa accagatgtg acttatgctg atgcagttga aaatatcgat attggtgggc
    13441 cgtctatgct tcgttcagca gcgaaaaatc atgccagtgt tacagttgtg gtagatcctg
    13501 ctgactacgc tgtggttttg gacgaattgg cagcaaacgg cgaaacctct tatgaaactc
    13561 gccaacgttt agcagccaaa gtatttcgtc acacagcggc ttatgacgcc ttgattgcag
    13621 aatacttcac agctcaagtg ggtgaaagca agcctgaaaa actcactttg acttatgacc
    13681 tcaagcaacc aatgcgttac ggtgagaatc ctcaacaaga cgcggacttt taccagaaag
    13741 ctttgcctac agattactct attgcttcag ccaaacagct caacgggaaa gaattgtcat
    13801 ttaataatat ccgtgatgca gatgctgcta tccgtatcat ccgtgacttc aaagatagtc
    13861 caaccgttgt ggctctcaaa cacatgaatc catgtggaat tggtcaagct gatgacatcg
    13921 agactgcttg ggactacgct tatgagtctg acccagtatc tatctttggt gggattgtcg
    13981 tcctcaaccg tgaggtggat gctgcgacag ctgagaagat gcacggcgtt ttcctcgaaa
    14041 tcatcattgc accaagctat acggatgaag cgctagccat tttgatcaat aaaaagaaaa
    14101 acttgcgtat ccttgccttg ccatttaatg ctcaagaggc tagcgaagtg gaagcagaat
    14161 acacaggtgt agtcggtgga cttctcgtgc aaaatcaaga cgtggtcaag gaaagcccag
    14221 ctgactggca agtggtgact aaacgtcagc caactgagac agaagcgact gctcttgagt
    14281 tcgcttggaa ggctatcaag tacgtcaaat caaatggtat tatcgtgacc aacgaccaca
    14341 tgacacttgg tgttggtcca ggtcaaacca accgtgtggc ttctgttcgc cttgccattg
    14401 accaagccaa agatcgtctg gacggggcgg tccttgcttc agatgccttc ttcccatttg
    14461 cggataacgt ggaagaaatc gccaaagcag gaattaaggc catcatccag cccggtggct
    14521 ctgtccgtga ccaagaatcc atcgaagcag cggataaata cggcttgact atggtcttta
    14581 caggtgtgag acattttaga cattaagaag ataaaaggga agaaaacagt ttctttcctt
    14641 ttttggctta aaatgctaac tgaaacaaga ttaaaacgaa cttttttgat ataatgttgg
    14701 taaataattc gcaaaagagg ttgaggaatg aaactgcttg ttgtcggttc tggtggtcgt
    14761 gagcatgcga ttgctaaaaa gttacttgaa tcaaaagacg tggaaaaagt ctttgtagct
    14821 cctgggaatg atgggatgac tctggatggt ttggaattgg taaatatctc tatttccgaa
    14881 cattctaaat tgattgactt cgcaaagacc aatgatgttg cttggacctt tatcggtcca
    14941 gatgatgccc ttgctgctgg catcgtggat gattttaacc aagctggact taaggccttt
    15001 ggtccgacta gggctgcagc ggagctggag tggtccaagg atttcgccaa ggaaatcatg
    15061 gtcaaatacg gcgttccgac agcaatatat ggcacatttt cagatttcga ggaagccaag
    15121 gcctatatcg aaaagcatgg tgcgcctatc gtagtcaagg cggatggctt ggcacttggg
    15181 aagggtgtcg tcgttgctga gacggttgag caagcggtcg aagccgctca tgagatgctt
    15241 ttggacaata aatttggtga ctcaggtgcg cgcgtggtta ttgaggaatt ccttgaagga
    15301 gaggaatttt cactctttgc ctttgtcaat ggtgataagt tctacatcat gccaacggct
    15361 caggaccaca aacgtgccta tgatggcgac aaagggccta acacgggtgg tatgggtgcc
    15421 tatgcgccag ttccacactt accacagagt gtagttgata cagcggttga caccattgtc
    15481 aagccagttc tagaaggggt gattaaagaa ggtcgtcctt atctgggagt tctttacgca
    15541 gggcttatcc tgacagctga tggaccgaaa gtcattgagt tcaacgctcg gttcggagat
    15601 ccagaaactc agattatctt gcctcgcttg acctctgact ttgctcaaaa tatcacagat
    15661 atcctggata gcaaggagcc aaatatcatg tggacggaca agggtgtgac tctgggtgtg
    15721 gttgtcgcat ccaagggcta cccgctagac tatgaaaggg gcgttgagtt gccagccaag
    15781 acagaaggcg atgtcatcac ctactatgca ggggctaagt ttgcggaaaa tagcagagca
    15841 ctgctctcaa atggcggacg agtttatatg ctcgttacca cagcagatac cgtcaaagaa
    15901 gcccaagcca gcatctacca agaactatcc caacaaaaaa tagaaggact cttctaccga
    15961 acagatatcg gaagcaaggc aattaagtaa agatataaga ataacgcgcc gtagtcgcca
    16021 aacacgataa tggtcgtcgt ggtgaaaaga ccagaacagt gaatgttctg gtcaggggga
    16081 aacttggaga ccttaggctc aaagtttagg aatgaaaccg aaggtttgct tccgcctcca
    16141 tcacctaaga tcattatcaa aaagaaaaat aaaaattcac aaaatacgtt aatgatcgta
    16201 tggtttgcga gcgttagcga gctaatatag aacaatcacc gccgttgtga aagaacgatt
    16261 ggatgataat ccaatcgttc agggaaattg gaagaccttg ggtttccaat ttaggcatga
    16321 gacacctttg gtggctgctg ccgtccctca caagctaagg tgattgttga aaaagaggaa
    16381 aaaggagaag aaatgaaacc agtaatttcc atcatcatgg gctcaaaatc cgactgggca
    16441 accatgcaaa aaacagcaga agtcctagac cgcttcggtg tagcctacga aaagaaagtt
    16501 gtttccgcac accgtacacc agacctcatg ttcaaacatg cagaagaagc ccgtagtcgt
    16561 ggcatcaaga tcatcatcgc aggtgctggt ggcgcagcgc atttgccagg catggtagct
    16621 gccaaaacaa cccttccagt cattggtgtg ccagtcaagt ctcgtgctct tagtggagtg
    16681 gattcactct attctatcgt tcagatgccg ggtggggtgc ctgttgcgac catggctatc
    16741 ggtgaagctg gagcgactaa cgcagctctc tttgccctcc gtctcctctc tgtagaagat
    16801 aagtccattg cggatgcact tgccaacttt gctgaagaac aaggaaaaat cgcagaggag
    16861 tcgtcaaatg agctcatcta aaacaatcgg aattatcggt ggcggtcaac tgggtcagat
    16921 gatggccact tctgctatct acatgggcca caaggttatc gcgctggatc ctgcggcgga
    16981 ttgcccggcc tctcgcgtgg cggaaatcat tgtggcacct tataacgatg tggacgccct
    17041 ccgtcagttg gcagaccgtt gcgatgtcct cacttacgag tttgaaaatg tcgacgctga
    17101 cggtttggat gccgttatca aggatggaca actccctcaa ggaacagatc tgctccgcat
    17161 ttcgcaaaat cgtatttttg aaaaggactt tttgtcaaac aaggctcaag tcactgtggc
    17221 accctacaag gtcgtgactt ctagcctaga cttggcagat atcgacttgt cgaaaaacta
    17281 tgtcctcaag actgcgactg gtggctacga tggtcatgga caaaaggtta ttcgttcaga
    17341 agcagacttg gaagcagcct atgcgctagc agactcagca gactgcgtct tggaagaatt
    17401 tgtcaacttt gaccttgaga tttctgtcat cgtgtcagga aatggcaagg aggtgacgtt
    17461 tttcccagtt caggaaaata tccaccgcaa caatatcctg tctaagacca tcgtaccagc
    17521 ccgcatttct gaaagtctag cagacaaggc taaagctatg gcagtgcgaa tcgcagaaca
    17581 actcaacttg tctggaactc tctgtgtgga aatgtttgcg actgatgatg acgttattgt
    17641 caatgaaatc gccccacgac cacataactc tgggcactat tctattgaag cctgtgattt
    17701 ctctcagttt gacacccata ttctgggtgt tctcggagca ccattaccag tcatcaaact
    17761 ccatgcgcca gccgttatgc ttaatgtcct cggtcagcat gtcgaggctg ctgagcaata
    17821 tgtgacagaa aattcaagcg cccacctcca catgtatggt aaaatagaag cgaagcacaa
    17881 ccgcaagatg ggacatgtga ccttgtttag tgatgtaccg gatagtgtgg atgagcttgg
    17941 ggaagggatt gatttttagg acaagtctat gatacaaatt atcgttaata catttattga
    18001 aaaggataag actggagcag ttgttgaagt gttgtatgcc agtgctgacc aagataaggt
    18061 acaagctaaa tatgaagaac tagctgcaca ataccccgaa aattatttag ctatctatga
    18121 tgtaccgctg gatacggatt tgaatacact agatcattac ccgtctgtgt ttattgaaaa
    18181 agaggagttt gagtagaaat cttggtttac ctagatagct tattcccaac agcttaagaa
    18241 gaaaggaaaa attaacacat gatcaaccgt tactctcgcc ctgagatggc gaatatttgg
    18301 agtgaagaaa ataaataccg tgcttggctt gaggtggaaa tcctctctga cgaggcatgg
    18361 gctgagttgg gggaaatccc taaggaagat gtggctttga ttcgcaagaa ggcggacttt
    18421 gacatcgacc gtattttgga aattgagcag gagacgcgcc acgatgtggt ggctttcacg
    18481 cgtgcggttt ctgagactct tggtgaagag cgcaagtggg ttcactatgg gttaacttct
    18541 accgacgtgg tggatactgc ttatggttac ctctacaagc aggccaacga catcatccgt
    18601 cgtgaccttg aaaacttcac taatatcatc gctgacaagg ccaaggagca caagttcacc
    18661 atcatgatgg ggcgtactca tggtgtgcac gctgagccga caacctttgg tcttaaattg
    18721 gcaacttggt acagcgaaat gaaacgcaat atcgagcgtt tcgagcatgc agctgctggt
    18781 gtagaagctg gtaagatttc tggtgcggtt gggaactttg ccaatatccc accatttgta
    18841 gaggagtatg tctgcgataa acttggcatc cgtgcccaag aaatctctac acaagtcctt
    18901 cctcgtgacc ttcacgctga gtactttgcg gttcttgcca gcattgcgac ttcaatcgaa
    18961 cgtatggcga ctgagattcg tggtctacaa aaatctgagc aacgcgaagt agaagagttc
    19021 tttgctaaag ggcaaaaagg gtcttcagca atgcctcaca aacgcaaccc aatcggttct
    19081 gaaaatatga ctggtctggc gcgtgtcatt cgtggtcaca tgattacggc ttatgaaaac
    19141 gtcgctctct ggcacgaacg cgatatttct cactcatcag ctgagcgtat catcacacca
    19201 gatacgacca ttttgattga ctacatgctc aaccgttttg gaaatatcgt caagaacttg
    19261 acagtcttcc cagaaaatat gatccgaaac atgaactcga cttttggtct tatctttagc
    19321 caacgggcta tgttgacatt gattgaaaaa ggcatgaccc gtgagcaagc ctatgacttg
    19381 gtgcaaccaa aaacagccta ctcttgggac aaccaagtag actttaaacc acttcttgag
    19441 gcagattcag aagtaacatc acgtctcact caagaagaaa tcgatgaaat cttcaaccca
    19501 gtttattaca ccaaacgagt ggatgatatc tttgaacgtc ttggactagg tgattaatta
    19561 aaaaataaac agcgagcttc aatctcgctg tttatttttt atcgaaaaga cttagtcttc
    19621 ttttctttta gtgagtccat aggctgctag tgtggccatg agtcctgcga ctactagtcc
    19681 tgcagaatcg tgagttcctg tttcaggaag ttttttctct attaccacag gagctggatc
    19741 ttgaggaaga gctttgcttt cctcagcagg agcagttgat ggagctggtt ggcttgggat
    19801 ttctagtttt ggtttttctt cagcaatagc ggcttgtccg ttttcatcgc ctacatgtgt
    19861 taccatagtt ccgacttcga ctatttgagt aacggcttcc tgtgctacga cactatttac
    19921 aagtgttttc acttccttac catcggcaga agtgctcaca gagtagaagt tgctacgatg
    19981 tccattgacg cccttagtaa tgacttgtgt ttttcctttg agtaagagtg gattttcacg
    20041 agtcactgtg gtaaatggaa tttcttcttt ttggatatcc agtctaggtt ttacctcagt
    20101 agttggtgca agaccacttt catcaccctt gtgagttaca ggagcgccaa cttcaaccac
    20161 ttggtttata acttctttgg ttacctggct atcaaggact gtttctgttg tttttccatt
    20221 ttcagtgagt acagagatgt aatgagttcg ctcacctttg actcctgctg tgataatatt
    20281 ttcctgacca gctgggaggt taggattttc tttcttgata acttcaaatg gaatttcttc
    20341 agttcttgtg atgagttctg gtctggtttc aacattggca gccacttcat tttcatccag
    20401 gcttcctgaa tgagttgcag ctggtttgag gccttgaaga gcggctttta ggttggctac
    20461 aagtgtgtca agctcagctt gtttattacg gttgaggttg taatttagag ctgttttagc
    20521 tgcgtcaagg gcctcaagac tttctttact atatccttct aagtttgtag gaattttagc
    20581 taattcttcg cggagagcat tataattagc acggaagtag tctttgttgt ggtctgcaaa
    20641 ggcagtcatg agttcaaaga tttcctcttc cttgtattca gcgcttggtc tatctgccca
    20701 gattgaaagc atacttccga ctgttggaag atctacttca ggatatttgg tagaagctag
    20761 ttgattgaat ggtgtttttc cagtattctc aatagctttc ttgaggaaac caccaccatc
    20821 ttctggtttt tgaccaagaa tgtagtacca gtcaccgttg gtattcaaga atttatagcc
    20881 tttgcttgct aggtattgag gtgatgcgag gttatatccc caccagcctt tagaccagta
    20941 agaaatcaag acatctttgt caaactgaac atcgtccttg tcttcatagt agaagccatc
    21001 gttgaaggcc attggttgaa gccctctttc tttggccata gctgcgaggg tgttggcata
    21061 ttcggcaaat ttgccataga gttgatacca cttgaggtag taccagcctt gggcactagt
    21121 cgcatcgttg gcgtattcgt cagtaccaaa gttgaaaatc tttgttttac ctgcaaagaa
    21181 gtccatgtat ttaccgatga gggcttttac aaagttcatc gcttcttcgt ttttcaagtc
    21241 catagttgtt tttgaaactt tatcaaagtg ggcttgagga tttttaatac ctaatttttc
    21301 catggcaacc agcatagcat ccatgtgacc tggactgtta atagctggga tgagaccgat
    21361 gtccttagat ttagcgtatt caattagctc tgttacttct gcctgtgtta gtgcagtacc
    21421 gtttggatcg tcgtagtaag ctttagttcc ttcgataata gcttttttaa cgtcatcact
    21481 agcataggtt tttccgttgg cagtaatggt catatcatcg agtagaaagc gaagtccgtc
    21541 atttcctaga aggagatgga catcagaata tccgagctca ctggccttgt ctacgatgcg
    21601 tttgagctgg ttcagagtaa agtatttgcg tccagcatcg attgagatta ccttgttttt
    21661 ggcaagtttt tcaacctcac gtttagcttc ttcttctttt tgagcttcag gcgtgagggt
    21721 caagttgttg acagtttctt gaagtttagc aatggcttga tcaatcgtat cttgttgggc
    21781 acggctaagg ttgctatcga gagagcgaat agctttttca gcttctttta cggccgcgac
    21841 gctttctgca gtataacggt tcaggtcttt tggtacctcg ttaagtgctt gctctgcaga
    21901 ttcataatca gctgcgaagt attcagcgtt ggcatttgca aaatgacgca tgagtttgaa
    21961 gaggtgtgat ggtgaataac gtgcagatgg agtgtcagcc caagcagcta ccataccacc
    22021 gatgattggg atatcagctc cttctgtttt tggtacagaa gtgattggtg tgtttttaat
    22081 accattgagc ccctgatcga gattgtacca gccttggcca tcagcgtttc gtccaagaac
    22141 gtagtaccaa gcatcattgg tattaaggat ttggtgacct ttttcagcta gtagtttaga
    22201 agaagcgaca tcgtagcctc cccaaccacc agtccacata gaaacgatga tgtctttgtc
    22261 aaaactacca aagcttgtgt cgctattgta gtagataccg tcgttaaagg ccattggttt
    22321 gagaccgtgc gattttacaa tacgagcgag gtcattggcg taggcaataa atttttcata
    22381 gccttttaca gggtagcctt cgtttggata gtatttatca gcttgaagca cactccaacc
    22441 tttagcatct gtcgcatcat tggcatattc atcaagtccg atgttgaaga tttcagtctt
    22501 tttcgcgaaa taagcagcat acttgtcgat aagggctttt gtaaaagcga cagcttgttc
    22561 gttgtcaaga tcgacagtac gggctgattt cttcccaaaa tagctaaagt tagggttttg
    22621 gattcccaat tctttcatgg cattgagaat cgcatccatg tgtccaggac tatttactgt
    22681 cggaatgaga ccgatacctt tatctttggc atagttaatc agatctgtca tttgactttc
    22741 tgttaagtga ttgccgtttg gatcgttgta ataatcattt gtaccttttt caatggcgcg
    22801 tttgacatcg tcactggcat aggtcttgcc gttagctgtg atgctcatat cgtccaacat
    22861 gaaacggagt ccatcatttc cgactaatag gtgtaaatca gtgtagccat aatgtttcgc
    22921 tttatcgatg atttccttga gctgttctgg tgagaaatat ttacgtccag catcaataga
    22981 aacaattttc tttttcgcta gtttttcatt tacagttgca gcacgttcct ttcctgtctc
    23041 tgtttccggt ttgtcagcct ctgctttcgc ttcatctttt ttagctggtt tatccttgtc
    23101 agtcttgtct gtatttgact ctttagaatc aacctctttc gcttcttcct ttttagggct
    23161 agcttcttct tcctttttat tagcagtttc tttttcagca gaagttggag ttaccacctc
    23221 tgctttatca ctaggagttg aactaacttc ctcttgtggt ttttcttctg tttttggaag
    23281 actagctacc ttatcagtag ctggagtttc tgtttctaca gtttttggag cttctggttg
    23341 aagctctgct ttaggtgttt cctcagtccg attttcggat gattgagggg aatcagaaac
    23401 cgtatggatg gtcggttggt tttctgtagt agtaggagta actccatcgg ctgcaacagt
    23461 ctgtgcttgg aaggcaaatc caattagaac agaagctgct cctacagcgt atttacgaat
    23521 agaaaaacgc tgttgttttt catgtttcat tgcaaaacct cctgattgca ttgttatatt
    23581 gatagcgatt atataaatca acgcctttat tttatttctt atattaacga gagtcaagag
    23641 gagatgacaa aaaactataa taagtataaa aaaatataaa atttaaactt aagatttcag
    23701 attggtcgaa aaaatacata tatatatcta gtataatttt tggttctatt tctataaaat
    23761 attccacaaa ttatagaatc ttccaaaaat aggtaagcgc tacctttttg gtgtggtata
    23821 ataagcatag aaaaagcccg agcgattagg ctcaggcttt cttcttagtg atcacggtca
    23881 catgagatga atttaatctt gtagtaatca gatcgtttgt aagtttcact gtattctaaa
    23941 acttggccag ttgattcgag tttggtgatt ttagtttgta ggacagtagg gaattgttca
    24001 tcgactccga ggactgaagc cgcatgttct ggagttggaa agactatttc gttgatttct
    24061 tcaaagtgtt catcattcat gtgaatgtgg tagtctaact tgaaacgatt atagatagaa
    24121 ctatagtatt caagatttgg ataatttgcg ttgatatatt gttctgggat gtaggatgta
    24181 tggtagatat aaacgacacc gtttgattcg cggatacgtt caatcttgta gtagaattga
    24241 tcgccgcgta gacccaattt ttccaagtaa acaagcttgt ttccgcgttc aattgaaaga
    24301 acagttacct tatcatcttt agcattgaag agttcaatat ctgaaaactc tacaagcttg
    24361 tgtttgcgtg cacgtgaaat gaaggttcct tttccttgtt ggcggacaat atagccatct
    24421 ttggcaaggt cgtttaaggc gcgaacaact gtgatagagc tgacatcgta cattgaaatg
    24481 agttctgctt cagtgtaaaa tttatctcca ctgctaaact gcccagagat gattttattt
    24541 tttaattcgt cttttatgta ttgatatttg ggaattgcca tatttttacc tctattttcc
    24601 ttctccatag aaaattttac cataaaagcg aaaaaaatag tagtcttttg aataaaaaat
    24661 tagaataata gtttgaaaaa ttaagcgata tagggattgc tcatatttta taaatgcatt
    24721 ctatacaaaa ttattttagg tactatattc caaatttaaa gagcgtttga aatgggtcta
    24781 aaaatgtttg aaactaattg aatgtgtgta taatagtaaa aatgcagtaa atatcccgac
    24841 aatttctatt gacaattcaa acagattggt ttataattaa tataacaaca aatgaaagcg
    24901 caaactttcg cggtcggaag gtagttttat gacacgattt gagatacgag atgatttcta
    24961 tctcgatgga aaatcattta agattttatc tggtgccatt cattatttta gggttcctcc
    25021 agaggattgg tatcattcgc tctataactt gaaggctctt ggttttaata cggtagagac
    25081 ttatgttgct tggaatttac acgagcctcg tgaaggtgag tttcattttg aaggtgatct
    25141 ggatttagag aaatttctcc aaatagcgca ggatttgggt ctctacgcaa ttgtgcgtcc
    25201 gtctccattt atctgtgcgg aatgggaatt cggtggctta ccagcttggc tcttgaccaa
    25261 gaacatgcga attcgctcat ccgacccagc atatatcgag gcagttggtc gctactatga
    25321 tcagttattg ccaagactgg tgcctcgttt gttggacaat ggtggcaata ttctcatgat
    25381 gcaggttgaa aatgagtatg gttcttacgg agaagataag gcttacctga gagcgattcg
    25441 acagctaatg gaagagtgtg gcgtaacctg tcccctcttt acatcagatg gtccatggcg
    25501 agctactctg aaagctggaa ccttaattga agaggacctc tttgtaacag gaaactttgg
    25561 ttctaaggca ccttacaact tttcgcagat gcaggaattc tttgatgaac atggtaagaa
    25621 atggccactc atgtgtatgg agttctggga tggttggttc aatcgctgga aagaaccgat
    25681 tatcacacgg gatcctaagg aattggcaga tgcagttcga gaggttttgg aacaaggatc
    25741 tatcaatctt tacatgttcc acggtggtac aaactttggt ttcatgaatg gttgctcggc
    25801 tcgaggaact ttggacctgc cacaagttac gtcttatgat tacgatgccc ttctggatga
    25861 agaaggaaat ccaactgcta aatatcttgc agtcaagaag atgatggcaa cacatttttc
    25921 agagtatccg cagttggaac cactctacaa agagagtatg gagttggatg ctattccact
    25981 agttgaaaaa gtttctttgt ttgaaacctt agatagcttg tcaagtcctg tagaaagtct
    26041 ctatcctcaa aagatggagg agctgggaca aagttatggc tacctacttt atcgaacaga
    26101 aacaaactgg gatgcagaag aagaaagact tcgtatcatt gatggtcgag atagggccca
    26161 gctgtatgtc gatggtcagt gggttaaaac tcaatatcag acagagattg gggaagatat
    26221 tttttatcaa ggtaaaaaga aagggctatc taggttagat atcttgatag aaaatatggg
    26281 gcgtgtcaac tatgggcata agttcttagc ggatacgcaa cgtaagggaa ttcggacagg
    26341 ggtctgtaag gatctgcatt tcttactaaa ctggaaacac tatccactcc cactagacaa
    26401 tcctgagaaa attgattttt caaaaggatg gactcaagga caaccagcct tttacgctta
    26461 tgactttaca gtcgaagagc caaaagatac ttacctagac ttgtctgagt ttggtaaggg
    26521 ggttgccttt gtcaatgggc agaatctagg acgtttttgg aacgttggcc caactctctc
    26581 actttatatc cctcatagct atctcaagga aggtgccaac cgcatcatta tctttgaaac
    26641 agaaggtcaa tataaagaag agattcattt aactcgtaaa cctacactaa aacatataaa
    26701 gggggaaaac ttatgacaat tgtaggatgc cgtattgatg gacgtttgat ccacggacaa
    26761 gtagccaatc tttgggctgg aaaactaaat gtttcacgca ttatggttgt agacgacgaa
    26821 gttgtcaaca acgatattga aaagagtggt ttgaaacttg cgacaccacc aggtgtgaaa
    26881 ttgagtattt tgccagttga gaaagctgca gccaatattc ttgctggcaa atacgatagc
    26941 caacgtctct ttatcgtggc tcgtaaacca gaccgcttcc ttggtttggt agaagcaggt
    27001 gtaccacttg aaacccttaa tgttgggaat atgtctcaaa caccagaaac tcgttctatt
    27061 acacgttcta tcaacgtagt agacaaggat gtggaagact tccacaaact ggcagaaaaa
    27121 ggtgttaaac ttactgctca gatggttcca aatgatccaa tttcagactt tttgagctta
    27181 ttaaaatagg aaaaaaattt ttaggaggtc attgttatga tacaatggtg gcaaatttta
    27241 cttctcactt tgtactcagc ttatcaaatc tgtgatgagt tgacgatcgt ttcatctgca
    27301 ggttcccctg tatttgctgg tttcattact ggtttaatca tgggagatgt gactactggt
    27361 ttacttatcg gtggtaactt gcaactgttc gttcttgggg ttggtacctt cggtggtgct
    27421 tctcgtatcg acgcaacttc tggtgcggtt cttgcgacag ccttctctgt ttcacaagga
    27481 attgatgcac cgcttgccat tactacaatc gctgtaccag tagcagctct cttgacttac
    27541 ttcgacgttc ttggtcgtat gactactacc ttcttcgctc accgtgtgga tgctgcaatc
    27601 gaacgctttg actataaagg tattgaacgc aactacttgc ttggtgcgat tccgtgggct
    27661 ctatctcgtg cccttccagt cttctttgcc cttgcttttg gtggtgcctt tgtacaatca
    27721 gtagtagact tcgttgaagc ctacaaatgg gttgcagatg gcttgacact tgcaggacgt
    27781 atgcttccag gtcttggatt tgcaatcttg cttcgttacc ttccagttaa acgtaacctt
    27841 cactaccttg ctatgggatt tggtttgaca gctatgttga ctgttcttta ctcatatgta
    27901 acaggtcttg gtggcgctgt tgctggtatc gtaggtactc ttcctgctga agttgctgaa
    27961 aaaattggtt tcgtgaacaa cttcaaaggt ttgtctatga ttggtatttc tatcgtaggt
    28021 attttccttg cagtgcttca cttcaaaaat agccaaaaag tagctgtagc agcaccttct
    28081 acaccatcag aaagtgggga aatcgaagat gacgaattct aattacaaac ttacaaaaga
    28141 agattttaat caaatcaaca aacgtagctt gtttactttc caattaggtt ggaactacga
    28201 acgtatgcaa gcttctggtt acctttacat gatcttgcct cagttgcgta aaatgtatgg
    28261 tgatggaact cctgaattga aagaaatgat gaaagttcat actcaattct tcaatacttc
    28321 accattcttc cataccatta tcgctggttt tgaccttgcc atggaagaaa aagatggtgt
    28381 aggttcaaaa gacgccgtta acggtatcaa gacaggtttg atgggaccat tcgctcctct
    28441 tggggataca atctttggtt cacttgtacc tgctatcatg gggtcagtcg cagcaactat
    28501 ggctatcgct ggccaacctt gggggatctt cctttggatt gcagttgcag tagcgtatga
    28561 catcttccgt tggaaacagt tggaatttgc ttacaaagaa ggggttaacc ttatcaacaa
    28621 catgcaaagt accttgacag ctttgattga cgctgcatct gtacttggtg tcttcatgat
    28681 gggtgctctt gtagcaacag tgattaactt tgaaatttct tacaagttgc caatcggtga
    28741 aaagatgatt gatttccaag acatcttgaa ccaaatcttc ccacgtttgc ttccagcaat
    28801 ctttactgcc tttatcttct ggttgcttgg taagaaaggt atgaactcta ctaaagctat
    28861 cggtattatt atcgtacttg ctttggctct ttctgccctt ggtcactttg cacttggaat
    28921 gtaattcctt atgactaaat cattaatttt ggtgagccat ggtcgcttct gtgaggagct
    28981 tagaggtagc acagaaatga ttatgggccc acaagacaac atttacacag tagctcttct
    29041 tccagaagat ggcccagaag aatttactgc taaatttgaa gctgttattg aaggattgga
    29101 tgatttccta gtctttgcgg atcttctcgg tgggacacct tgtaatgtgg tgagtcgctt
    29161 gatcatggaa ggtcgtgata ttgaccttta cgcagggatg aatcttccaa tggtgattga
    29221 atttatcaat gcgagcctta caggcgcaga tgcggactac aagagccgtg ctgcagaaag
    29281 cattgtgaaa gttaatgacc tgttagcggg cttcgatgat gacgaagatg aataatactc
    29341 ttcgaaaatc tcttcaaact acgtcaacgt cgccttgccg taggtatatg ttactgactt
    29401 cgtcaatctt atccggcaac ctcaaaacgg tgttttgagc tgacttcgtc agttctatct
    29461 gcaacctcaa agcagtgctt tgagcagcct gcggctagtt tcctatttta gttggaactc
    29521 gattcaattc atgtgacaac gtgaaaatcg ttagagcatt ttatatagaa tatacatggg
    29581 aatggagctt actcccattc ccatatttaa tagaaaaaga ggaactcaat gctacattat
    29641 acaaaagaag acttgctcga attgggtgca gaaatcacta cgcgtgaaat ctaccaacaa
    29701 cctgatgtat ggagagaagc ttttgaattt tatcaagcaa aacgtgaaga aattgcagcc
    29761 ttcctacaag aaatcgctga taaacatgac tatattaagg ttatcttgac aggtgctggg
    29821 acttctgctt atgtgggaga caccttgcta ccttacttta aggaagtcta tgacgaacgc
    29881 aaatggaatt tcaatgctat tgcgacaaca gatatcgttg ccaatccagc aacctatttg
    29941 aaaaaagatg tggcaactgt ccttgtgtct tttgctcgta gtgggaactc gcctgaaagt
    30001 gtggcgactg ttgatttggc caaatccttg gtggatgagc tttatcaagt gacgattact
    30061 tgtgcagcag atggtaaatt ggctcttcaa gctcacggtg atgaccgtaa tctcttgctc
    30121 ttgcaaccag ctgtctctaa tgatgctgga tttgccatga cttctagctt tacgtctatg
    30181 atgttgacag ctctcttggt ctttgatcct acagaatttg ctgttaagtc tgaacgtttt
    30241 gaagttgtat ctagtcttgc ccgtaaagtt ttagacaagg cagaagatgt caaagagctt
    30301 gttgatttag actttaaccg tgtcatctat ctaggcgctg gtcctttctt tggacttgct
    30361 catgaagctc agctcaagat tttggaatta actgctggtc aagttgcgac catgtatgaa
    30421 agcccagttg gcttccgtca cggtccaaaa tctcttatca acgacaatac agttgttttg
    30481 gtctttggta caacgacaga ctacactcgt aagtacgact tggacttggt tcgtgaagtt
    30541 gctggtgacc agattgctcg tcgtgttgtg cttttgagtg atcaagcttt tggtcttgaa
    30601 aatgtcaaag aagtggccct tggttgtggc ggtgtcttga atgatattta ccgtgtcttc
    30661 ccttacatcg tttatgccca actctttgcc ctattgactt cactcaaggt agaaaataaa
    30721 ccagatacac cgtctcctac aggtacagta aaccgtgtag tacaaggtgt cataattcac
    30781 gaatatcaaa agtaagacag tgtttatgaa ttcttgacaa gaggatttgt aaattatcag
    30841 ataaaccata gattgtcagt acgctttcta tggtttgttt gcttgagaga aatagtaaaa
    30901 ggagaacaga atgaaagcat acacagagcg tgtatttgga aatgttgagg gcgaggatgt
    30961 cttggcctat cgatttgaga cagacggtgg ctaccaactt gaggttatga cttatggtgc
    31021 gaccatcttg cgctatgtcg cacctgacaa ggctggaaat tttgccaatg ttatcttggg
    31081 atttgatgac tttgatagtt atgtaggcaa tagtcccaag catggagcaa gtgtaggtcc
    31141 tgtagcgggt cgtattgcag gtgcgacctt tgagctcaat ggtaagacct atgaccttga
    31201 ggttaataat gctagcaact gtaatcacag tggttcaact ggttgggatt ccagcttgtt
    31261 tgaagttgaa gaagtaagcg atcatggctt gactctctac acagagcgta cagatgggac
    31321 aggagggttc cctggaaatc tcaagatttg gatcagttat cacttggaag aaactggtgc
    31381 ctatgaaacc atctacaagg taacgaccga tcaggatacg ctagtcaatc caaccaacca
    31441 cagctatttc aacttgtctg gtgatttcac gcagacgatt gaccgtcatg tcttccaact
    31501 aaacacagag ggcatttact caatcgctcc tgacggtgtt cctgccaaaa ctccagaagc
    31561 caaccgtgat gtggtcaaac acatctacaa tggtaccttg ttgaaggata tctttgcaga
    31621 agaagatgag caaatccagc tggcatcagg tttggatcac ccatttgccc ttcctgcagg
    31681 ccatgacaat gctggattcc tttatgacca aaattcaggt cgcttcctgc ttttcaagac
    31741 agaagctcct tgctttgtgg tctacacagc aaactttgtg gatgaaagtg tcatcatagg
    31801 aggtcagcca atgctacagc acaatgggat tgctcttgaa gcgcaagctt taccagatgc
    31861 cattcacagt gaccttaaag gccaagtcat tcttaaagct ggtcaaacct tcaccagtaa
    31921 gacacgttat gaacttgttg tgaaataaaa aagctggtca aaaagtcttt aaaattaaaa
    31981 taacgcatat tgtcaggtga tgaaaagttt gatactatgc gttttattat gaaatgattt
    32041 actttatctt atcctgaaat ggtgttattt ttgagttatc tggcttgact ttcttgatgg
    32101 aaattatata atagttgtaa taacaataat tacaacgaga aaagaggacc atttatgacc
    32161 tatgaataca agagtcacat ttatttggca gagacagttt taaatgtaaa ggatttggcc
    32221 agtcaaacag ccttttatca gcaagttatt ggtttagaaa tcttatctca aactgagaca
    32281 gagtccattc taggccttgg tggaaaagtc ttggtacagc tgattcaagc acaagagagc
    32341 ggagaagtga gggaacatta tggtctttac catttggcca ttctcttgcc cacacgcaag
    32401 gctttagctg atgtcttgaa acacctgaca gatttacaaa ttcctcttgt cggcggtgca
    32461 gaccatggtt acagtgaagc tatttacttg gaagatttgg agggaaatgg cattgaactc
    32521 tatcgagata agccagtttc cacatgggat attcgagaag atggacgcat tatcggagtg
    32581 acagaggctc ttgcggcgca ggacatttat gagttggggg aaagagtaga gccctttatc
    32641 ctggcagaag gtacgagaat ggggcatatt catctttccg tcaaggatag tcgaaagtcc
    32701 agccagtttt atcaaaaggt gttagggcta gaggataaat tcagtgtgcc tagcgctagt
    32761 tggattgcgg ctggagatta ccatcatcat ttagcagtta acgaatgggg aggaaaaggt
    32821 ctggcttcgc gtaagcaagg cttaccaggt ttagcctact atgtcatcga agtcgcacac
    32881 aaaaaagaac tgttaacgat tgcccaacga gcacaagaag ttgacgcacc aatcaaatgg
    32941 atgacatcga gccaattgga aatcacagac tcagatggca tcgtgacccg tattcgttta
    33001 gcaaggtaaa attaggaatt tttaataagt tataagtatt gctagtttgc tttttgaacg
    33061 gtctttggta acagaactgc gtgagctgga tgggcgagaa ttttgacaaa gaagctgatt
    33121 agagatataa tgaagaaaaa tcgagcaagg aaaaagaaat gacaaattca aagtatatta
    33181 cttgtttgaa acgttcagag ggccagttgt gtgggattca aaagatgatt gaaggagatc
    33241 gtgactgtgc tgatattgtg acgcaactga cagcagtgag atctagtgta gagcgcgtga
    33301 ttgagatgat aattaccgaa aatcttactg aatgtatcaa tcagccacta gatgattctg
    33361 aagctcaaaa ggaacgccta gaaaaggcta tccgatactt gattaaacgg aaataaatga
    33421 atgttttgag acgatatgtg caaaggtcta ttaactatta gaatagttta atgtgagtca
    33481 aaagaagttg taaaaaatct ttaaaattaa aaaaacgcat agtatcaggt gttgaatgta
    33541 ctgaccccca aaagttagat tttttctgtc taacttttgg ggtgcagttc ataagaacct
    33601 tggtactatg cgttttatta tgtagagact tatactcttc gaaaatctct tcaaaccacg
    33661 tcagcttcac cttgccgtag atatggttac tgacttcgtc agttctatcc acaacctcaa
    33721 aacagtgttt tgagctgact tcgtcagttc catccacaac cttaaaacag tgttttgagc
    33781 agcttgcggc taacttccta gtttgcactt taattttcat tgagtattac ttcatcttct
    33841 catgaaattg agtttttgcc cagctcctct tgattattct acttgaccgg gccaagcatt
    33901 cataccacct tctacgttgg taacggtgag gccttgggag ctgagaaatt ggcaggtaga
    33961 tgcagagcgg actcctcctt gacaaatgac atagtattca tggtcaggtt tgagttcttt
    34021 gtagccttgc tctaaggtac ttaatggaag atttttggca cctggtgcat gtcctgcttg
    34081 gaattcatgt gcttcacgga catcgataag ttctagattt tcattttggt attttccata
    34141 aaagtcagcc atgctgatac tagtttccat attctactcc ttctgggtta accattttgt
    34201 atagtgaata agcgccgtca aggttttggg cggtaaatcc tgcttgcttg aggataggct
    34261 ctgcgatata gctgcgcaaa ccactgtggc agctgacgat gtaggcttgt tttttgtcca
    34321 gttcatcagt ttcctccttg acactttgtc agtttttgat acaatagtac aaaattagag
    34381 gaggcaggct atgattcaga aacatgcgat tcccatttta gagtttgatg ataatcctca
    34441 ggcggttatc atgcccaatc acgaggggtt ggatttgcag ttgccaaaga agtgtgtcta
    34501 tgctttttta ggtgaggaga ttgaccgcta tgcgagggaa gtaggggcga actgtgttgg
    34561 cgaattcgtt tctgctacca agacctatcc agtctatgtt atcaactaca agggtgagga
    34621 ggtctgtctg gctcaggctc ctgttggctc cgctccagca gcccagttta tggattggtt
    34681 gattggctat ggtgtggagc agattatctc tactgggacc tgtggtgtcc tagctgatat
    34741 agaggaaaat gcctttctag tccctgttcg cgctttgcga gatgagggag ccagttacca
    34801 ctatgtggca ccttgtcgtt atatggaaat gcagccagag gctattgctg ctattgagga
    34861 agttttggaa gacagaggga ttccttatga agaagtcatg acctggacga cagacggttt
    34921 ttaccgagaa acggctgaaa aggtggctta tcgtaaggaa gaaggctgtg ctgttgtgga
    34981 gatggagtgt tctgctcttg cggcagtagc tcaattgcgt ggggttctct ggggtgaatt
    35041 gttgttcaca gcagattctc tagcggactt ggaccagtac gacagtcgtg actggggctc
    35101 ggaagctttt aataaggcgc tagaactgag tttagcaagt gttcaccacc tttagttgta
    35161 ctggcaaagg atttgtttta tcataaaata tctagctcat acttttcaaa aatatgttta
    35221 aacgaggtca ccttcctctt gtcctaggca tgttgaggtt gggaaaaatc tttaaaatca
    35281 gaaaaacgta tcatatcagg tgatgaaaat tttgatacta tgcgttttat gttggtaaga
    35341 tttagagtga gatgaaatga tactcttcga aaatctcttc aaaccaggtc agcttc
//
LOCUS       DAVAAL010000009        35115 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVAAL010000009 DAVAAL010000000
VERSION     DAVAAL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35115
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.009"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..466
                     /locus_tag="VQM60_000316"
     CDS             <1..466
                     /locus_tag="VQM60_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triosephosphate isomerase"
                     /protein_id="HET1080215.1"
                     /translation="RRDYFHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAE
                     FVGAQVSAALAGLTAEQVAASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFG
                     QEVADKVRVQYGGSVKPENVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            504..2009
                     /gene="lytC"
                     /locus_tag="VQM60_000317"
     CDS             504..2009
                     /gene="lytC"
                     /locus_tag="VQM60_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HET1080216.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGVYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            2506..3024
                     /locus_tag="VQM60_000318"
     CDS             2506..3024
                     /locus_tag="VQM60_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET1080217.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            3156..3662
                     /locus_tag="VQM60_000319"
     CDS             3156..3662
                     /locus_tag="VQM60_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET1080218.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWYLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNSEEKIDGVTTFYDVQSVLDWYHAQDKNLYILGG
                     KQVFQAFEPYLDEVIVTQIHARVEGDTYFPEEFDLSLFETVSSKSFTKDEKNPYDFTI
                     QYRKRKEV"
     gene            3662..3832
                     /locus_tag="VQM60_000320"
     CDS             3662..3832
                     /locus_tag="VQM60_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080219.1"
                     /translation="MERSIFGFFTAMLCLVCLLAGAQAFRKKRYGLSVLLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            3869..5101
                     /gene="clpX"
                     /locus_tag="VQM60_000321"
     CDS             3869..5101
                     /gene="clpX"
                     /locus_tag="VQM60_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET1080220.1"
                     /translation="MPTTRNNDMMVYCSFCGKSQEEVQKIIAGNNAFICNECVELAQE
                     IIREELAEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELKFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            5110..5697
                     /locus_tag="VQM60_000322"
     CDS             5110..5697
                     /locus_tag="VQM60_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET1080221.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            5708..6088
                     /locus_tag="VQM60_000323"
     CDS             5708..6088
                     /locus_tag="VQM60_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET1080222.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            6140..7030
                     /gene="rapZ"
                     /locus_tag="VQM60_000324"
     CDS             6140..7030
                     /gene="rapZ"
                     /locus_tag="VQM60_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET1080223.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            7027..8004
                     /locus_tag="VQM60_000325"
     CDS             7027..8004
                     /locus_tag="VQM60_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET1080224.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            8001..8912
                     /gene="whiA"
                     /locus_tag="VQM60_000326"
     CDS             8001..8912
                     /gene="whiA"
                     /locus_tag="VQM60_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET1080225.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(8946..9914)
                     /locus_tag="VQM60_000327"
     CDS             complement(8946..9914)
                     /locus_tag="VQM60_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET1080226.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLNLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            10050..10907
                     /locus_tag="VQM60_000328"
     CDS             10050..10907
                     /locus_tag="VQM60_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET1080227.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            10894..11673
                     /locus_tag="VQM60_000329"
     CDS             10894..11673
                     /locus_tag="VQM60_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET1080228.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            11697..13049
                     /locus_tag="VQM60_000330"
     CDS             11697..13049
                     /locus_tag="VQM60_000330"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET1080229.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            13123..13497
                     /locus_tag="VQM60_000331"
     CDS             13123..13497
                     /locus_tag="VQM60_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET1080230.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            13499..14347
                     /locus_tag="VQM60_000332"
     CDS             13499..14347
                     /locus_tag="VQM60_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET1080231.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            14624..15391
                     /locus_tag="VQM60_000333"
     CDS             14624..15391
                     /locus_tag="VQM60_000333"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET1080232.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            15403..16587
                     /locus_tag="VQM60_000334"
     CDS             15403..16587
                     /locus_tag="VQM60_000334"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET1080233.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSLLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            16584..18455
                     /locus_tag="VQM60_000335"
     CDS             16584..18455
                     /locus_tag="VQM60_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET1080234.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(18598..19782)
                     /gene="mntE"
                     /locus_tag="VQM60_000336"
     CDS             complement(18598..19782)
                     /gene="mntE"
                     /locus_tag="VQM60_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET1080235.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            20086..22782
                     /locus_tag="VQM60_000337"
     CDS             20086..22782
                     /locus_tag="VQM60_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET1080236.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            22795..23586
                     /gene="yghU"
                     /locus_tag="VQM60_000338"
     CDS             22795..23586
                     /gene="yghU"
                     /locus_tag="VQM60_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET1080237.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            23702..24112
                     /locus_tag="VQM60_000339"
     CDS             23702..24112
                     /locus_tag="VQM60_000339"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET1080238.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            24124..25719
                     /locus_tag="VQM60_000340"
     CDS             24124..25719
                     /locus_tag="VQM60_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080239.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            25785..25949
                     /locus_tag="VQM60_000341"
     CDS             25785..25949
                     /locus_tag="VQM60_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET1080240.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            26028..26756
                     /gene="yaaA"
                     /locus_tag="VQM60_000342"
     CDS             26028..26756
                     /gene="yaaA"
                     /locus_tag="VQM60_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET1080241.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(26807..28291)
                     /locus_tag="VQM60_000343"
     CDS             complement(26807..28291)
                     /locus_tag="VQM60_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET1080242.1"
                     /translation="MRNLLSTKVQRQLRLMENLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPEITKKFICRNHLSIINLVNHLNTLSNEIHLSNTL"
     gene            28507..34128
                     /locus_tag="VQM60_000344"
     CDS             28507..34128
                     /locus_tag="VQM60_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET1080243.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAKENKQETTNSISVGRGEAATKLAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETN
                     PAATTDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDLDK
                     GYSGFRAAPAPTNKLVTNLGNNTVFTDISRGSHTFRGNGNSRGGNPIHFDVTTTRVGN
                     RVEFSIAYSGPGEFVNNNFLLDKGNGFGEPSRATISTNRLRDQTKDIRKGANFVSHSS
                     YSLTSAVETNSNQTIRFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNY
                     YFREKPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIY
                     KVHQLGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFY
                     LDNDGNGVYTEGVDELVQKIAIKDGAKGEKGVQGERGLTGAQGQAGRDGVTPTVTVKD
                     NKNDGTHTITINDGRGNVASTVVRDGFDGASPLVATQRNEADKTTTVIFYYDQNGNNE
                     LDASDKKLKEVIIADGAKGEQGLQGRDGAQGPKGEQGNPGTPGKDGKSLIAVKNGTET
                     KVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNGKNGVLVTITPVG
                     GRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTTTIRDGATGQAGR
                     DGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIKGPKGDKGADGAK
                     GEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQGKDGDKYVNTETG
                     DVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHSTDITFTVPGKDPV
                     TVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGKYTPGTDELLGSE
                     MIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPLAVIRDGVDGKSP
                     TVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGAIGQDGQTPTITTQRGQ
                     DGQSTVVTITTPGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRIRRATDAAATPTR
                     NGIRVTVYDDVNDNGVYDGGVDKVLNSKDIYNGIDGRDGSAPTITTKDNGDGTHTITV
                     QNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKETVVKNGKDGKTP
                     KVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPDGSHTVTITNPDG
                     TKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETTVRDGKSPKVTIT
                     DEQNGTHKISVLNGDGTTTETIIKDGKSPVATVRDNQDGTYTIRVENGNGTVSETTVR
                     DGKSPTAKVVDNGDGTHTITVVNSDGTTTTTTVRYGREPKLEVIDNNDGSHTIKVTGA
                     DGKETTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDGVSPTVT
                     VENNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGTVT
                     KTVIKDGKDGRDGRDGRDGKDGKCGCQDKPVTPSNDKPVPPAPNVPTPEVLVKPVPAQ
                     PTPNVPTPEVPVQPTPAVPTPEVPVPEQPVVPTPAQPATPVNANPVAPTTGKENRGDK
                     LPETGSQSDYISVLLGSGILLSLYVGRRKED"
     gene            complement(34365..34913)
                     /locus_tag="VQM60_000345"
     CDS             complement(34365..34913)
                     /locus_tag="VQM60_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET1080244.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGASALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
BASE COUNT        10767 a         6523 c         8012 g         9813 t
ORIGIN      
        1 acgccgtgac tacttccatg aaactgatga agatatcaac aaaaaagcaa aagcaatctt
       61 tgcgaacggt atgcttccaa tcatctgttg tggtgaatca cttgaaactt acgaagctgg
      121 taaagctgct gaattcgtag gtgctcaagt atctgctgca ttggctggat tgactgctga
      181 acaagttgct gcctcagtta tcgcttatga gccaatctgg gctatcggta ctggtaaatc
      241 agcttcacaa gacgatgcac aaaaaatgtg taaagttgtt cgtgacgttg tagctgctga
      301 ctttggtcaa gaagtcgcag acaaagttcg tgttcaatac ggtggttctg ttaaacctga
      361 aaatgttgct tcatacatgg cttgcccaga cgttgacggt gcccttgtag gtggtgcgtc
      421 acttgaagct gaaagcttct tggctttgct tgactttgta aaataatcag taagtagcaa
      481 aagctaggtg gaacagcatt cagatgtctg ttacattttt tataggagag gaaagattga
      541 aaataaaaat tggattagca agtatctgtt tactaggctt ggcaactagt catgtcgctg
      601 caaatgaaac tgaagtagca aaaacttcgc aggatacaac gacagcttca agtagttcag
      661 agcaaaatca gtcttctaat aaaacgcaaa cgagcgcaga agtacagact aatgctgctg
      721 cccactggga tggggattat tatgtaaagg atgatggttc taaagctcaa agtgaatgga
      781 tttttgacaa ctactataag gcttggtttt atattaattc agatggtcgt tactcgcaga
      841 atgaatggca tggaaattac tacctgaaat caggtggata tatggcccaa aacgagtgga
      901 tctatgacag taattacaag agttggtttt atctcaagtc agatggggtt tatgctcatc
      961 aagaatggca attgattgga aataagtggt actacttcaa gaagtggggt tacatggcta
     1021 aaagccaatg gcaaggaagt tatttcttga atggtcaagg agctatgatg caaaatgaat
     1081 ggctctatga tccagcctat tctgcttatt tttatctaaa atccgatgga acttatgcta
     1141 accaagagtg gcaaaaagtg ggcggcaaat ggtactattt caagaagtgg ggctatatgg
     1201 ctcggaatga gtggcaaggc aactactatt tgactggaag tggtgctatg gcgactgacg
     1261 aagtgattat ggatggtgct cgctatatct ttgcggcctc tggtgagctc aaagaaaaga
     1321 aagatttgaa tgtcggctgg gttcacagag atggtaaacg ctatttcttt aataatagag
     1381 aagaacaagt gggaaccgaa catgctaaga aaatcattga tattagtgag cacaatggtc
     1441 gtatcaatga ttggaaaaag gttattgatg agaacgaggt ggatggtgtc attgttcgtc
     1501 taggttatag cggtaaagaa gacaaggaat tggcgcataa cattaaggag ttaaaccgtc
     1561 tgggaattcc ttatggtgtc tatctctata cctatgctga aaatgagacc gatgctgaga
     1621 atgacgctaa acagaccatt gaacttataa agaaatacaa tatgaacctg tcttacccta
     1681 tctattatga tgttgagaac tgggaatatg taaataagag caagagagct ccaagtgata
     1741 cagatacttg ggttaaaatc atcaacaagt acatggacac gatgaagcag gcgggttatc
     1801 aaaatgtgta tgtctatagc tatcgtagtt tattacagac gcgtttaaaa cacccagata
     1861 ttttaaaaca tgtaaactgg gtagcggcct atacgaatgc tttagaatgg gaaaaccctt
     1921 attattcagg agaaaaaggt tggcaatata cctcttctga atacatgaaa ggaatccaag
     1981 ggcgcgtaga tgtcagcgtt tggtattaag caatgatttg aaagagggat gtgatagtag
     2041 caccctcttt ttctttgttt tatgatagtt catcctcgag taaattcaag atcttgctcg
     2101 gaaatgaagc ttatatagta gattgaatat agacaaatac cttgtgattg gtaaaacatt
     2161 ttagaaattc atttaccttt cctaatcgac ttggtttcat cttatttcaa tctattatag
     2221 tattggggaa tttcttcaaa ccacatcagc ttggtcagtt ctacctgcga cctcaaaact
     2281 tgtgctttgg tcaagctggg tttagtttcc tagtttgctg atggatttcc attgactata
     2341 agcatccaaa cctctttttt gtcttctaaa gaattcttaa attatcagtc tattgcaact
     2401 tttctcatat aagttctttg tcttgctatt ggttttcctt agtagtatac taaggtagta
     2461 atcattaaga agtggttaca aaaaataatg aatgaggtaa agaaaatggt agaattgaaa
     2521 aaagaagcag taaaagacgt aacatcattg acaaaagcag cgccagtagc attggcaaaa
     2581 acaaaggaag tcttgaacca agctgttgct gatttgtatg tagctcacgt tgctttgcac
     2641 caagtgcact ggtatatgca tggtcgtggt ttccttgtat ggcatccaaa aatggatgag
     2701 tacatggaag ctcttgacgg tcaattggat gaaatcagcg aacgcttgat tacactcggt
     2761 ggaagcccat tctctacatt gacagagttc cttcaaaata gtgaaatcga agaagaagct
     2821 ggtgaatatc gtaatgttga agaaagcttg gaacgtgttc tagctattta tcgctacctc
     2881 atcactcttt tccaaaaagc gttggatgtg actgacgaag aaggcgatga tgttacaaac
     2941 gatatttttg ttggtgctaa ggctgaactt gagaaaacag tttggatgct tgccgcagaa
     3001 cttggacaag cacctggttt gtaagaaata atagcaatat atataaatct gtaagaagtt
     3061 tcttacagat ttttttctat tctgtaagct attccaaacc agtctttttt tgagtttttt
     3121 gataaaatag tactatcagt gaaaaggatg gaagcatgac taagaaaatt gtagctattt
     3181 gggcccagga tgaagagggt gtgattggta aggacaatcg tctgccttgg tatttaccag
     3241 cagaactgca acacttcaaa gaaacaactc tgaatcatgc tatcttgatg gggcgcgtga
     3301 cctttgatgg gatggggcgt cgactgcttc caaaacggga gaccttgatt ttgacacgta
     3361 attcagaaga aaagatagat ggggttacta ctttttatga tgttcagtct gtcttggact
     3421 ggtatcatgc tcaagacaag aatctctata ttcttggtgg gaagcaagtt tttcaggctt
     3481 ttgaacccta ccttgacgaa gtgattgtga ctcaaattca tgctcgggtg gagggagata
     3541 cctatttccc tgaagagttt gacttgtccc tttttgagac agtttcaagc aaatccttta
     3601 ccaaagatga gaaaaaccct tatgatttta ccatccaata ccgcaagaga aaggaagtct
     3661 aatggaacgt agtatatttg gttttttcac agccatgctg tgtttggtct gcttacttgc
     3721 aggagcacag gcttttcgta aaaagcgtta tgggctttct gtcctgctct ggttaaatgc
     3781 ctttaccaat ttggtcaata gtatccatgc tttttacatg accttatttt agatagaatg
     3841 acagtataga atagaacgga aggaaatcat gcctacaact aggaataatg atatgatggt
     3901 ttattgctca ttttgtggca aaagccaaga agaagtacaa aaaataatcg cagggaacaa
     3961 cgcctttatc tgtaatgaat gtgtggagtt agcccaggaa atcattcgtg aggagttggc
     4021 tgaggaagtt ttggcagact tgtctgaagt tccaaaacca atcgaacttc tccatatctt
     4081 gaaccactat gtaatcggtc aagatcgtgc caaacgggcc ttagcagtgg cggtttacaa
     4141 ccactacaaa cgcatcaatt tccacgatac gcgtgaagag tcagaagatg tggatttgca
     4201 gaagtcaaac atcttgatga ttggcccaac tggttcaggg aaaactttcc ttgctcagac
     4261 cttggctaag agcttgaatg taccttttgc tattgcggat gcgacagctc tgacggaggc
     4321 tggttatgtg ggtgaggatg tggaaaatat ccttctcaaa ctcttgcagg ttgctgactt
     4381 taacatcgaa cgtgcagagc gtggcattat ctatgtggat gaaattgaca agattgccaa
     4441 gaagagtgag aatgtgtcta tcacacgtga tgtttctggt gaaggggtgc aacaagccct
     4501 tctcaagatt atcgagggaa ctgttgctag cgtgccgcct caaggtggac gcaaacatcc
     4561 acaacaagag atgattcaag tggatacaaa aaatatcctc ttcatcgtgg gtggtgcttt
     4621 tgatggtatt gaagaaattg tcaaacaacg tctgggtgaa aaagtcatcg gatttggtca
     4681 aaacaataag gcgattgacg aaaacagctc atacatgcaa gaaatcatcg ctgaagacat
     4741 tcaaaaattt ggtattatcc ctgagttgat tggacgcttg cctgtttttg cggctcttga
     4801 gcaattgacc gttgatgact tggttcgcat cttgaaagag ccaagaaatg ccttggtgaa
     4861 acaataccaa accttgcttt cttatgatga tgttgagttg aaatttgacg acgaagccct
     4921 tcaagagatt gctaataaag caatcgaacg gaagacaggg gcgcgtggac ttcgctccat
     4981 tatcgaagaa accatgctag atgttatgtt tgaggtgccg agtcaggaaa atgtgaaatt
     5041 ggttcgcatc actaaagaaa ctgtcgatgg aacggataaa ccgatcctag aaacagccta
     5101 gaggtgacta tggaacttaa tacacacaat gctgaaatct tgctcagtgc agctaataag
     5161 tcccactatc cgcaggatga actgccagag attgccctag cagggcgttc aaatgttggt
     5221 aaatccagct ttatcaacac tatgttgaac cgtaagaatc tcgcccgtac atcaggaaaa
     5281 cctggtaaaa cccagctcct gaactttttt aacattgatg acaagatgcg ctttgtggat
     5341 gtgcctggtt atggctatgc tcgtgtttct aaaaaggaac gtgaaaagtg ggggtgcatg
     5401 attgaggagt acttaacgac tcgggaaaat ctccgtgcgg ttgtcagtct agttgacctt
     5461 cgtcatgacc cgtcagcaga tgatgtgcag atgtacgaat ttctcaagta ttatgagatt
     5521 ccagtcatca ttgtggcgac caaggcggac aagattcctc gtggtaaatg gaacaagcat
     5581 gaatcagcaa tcaaaaagaa attaaacttt gacccgagtg acgatttcat cctcttttca
     5641 tctgtcagca aggcagggat ggatgaggct tgggatgcaa tcttagaaaa attgtgagga
     5701 aaagaaaatg gcaaaaacaa ttcatacaga taaggcccca aaggctatcg gaccctatgt
     5761 tcaaggaaaa atcgttggca accttttgtt tgctagcggt caagttcccc tatcccctga
     5821 aactggggaa attgtaggag agaatatcca agaacagaca gagcaagtct tgaaaaacat
     5881 cggtgctatt ttggcagaag caggaacaga ctttgaccat gttgtcaaaa caacttgctt
     5941 cttgagcgat atgaacgact ttgttccttt taatgaggtt taccaaacgg ccttcaaaga
     6001 ggaattccca gctcgttcag ctgtggaggt agctcgtctt cctcgtgatg taaaagtcga
     6061 aattgaagtc atcgcagaga ttggataagc tagttgaagt ttggtgttgc caaacttctt
     6121 ttgatataag gagaaaaaga tgacaaagaa acaacttcac ttggtgattg tgacagggat
     6181 gagtggcgca gggaaaactg tagccattca gtccttcgag gatctaggtt atttcaccat
     6241 tgataatatg ccgccagctc tcttgcctaa gtttttgcag ctggttgaaa ttaaggaaga
     6301 taatcctaag ttggctttgg tagtggatat gcgtagccgt tctttctttt cagagattca
     6361 agctgttttg gatgagttgg aaaatcaaga tggtttggat ttcaaaatcc tctttttgga
     6421 tgcggctgat aaggaattgg tcgctcgtta caaggaaacc agacggagtc acccactagc
     6481 agcagacggt cgtattttag atggaatcaa gttggaacgt gaactcttgg cacctttgaa
     6541 aaatatgagc caaaatgtgg tggatacgac tgaactcact ccacgtgaac tgcgcaaaac
     6601 ccttgcagag cagttttcag accaagaaca agcccagtct ttccgtatcg aagtcatgtc
     6661 tttcggattt aagtatggaa tcccgattga tgcggacttg gtctttgatg tccgtttctt
     6721 gccaaatccc tattatctac cagaactgag aaaccaaacg ggtgtggatg aacctgttta
     6781 tgattatgtc atgaaccatc ctgagtcaga agacttttat caacatttat tggccttgat
     6841 tgagccgatt ctgccaagtt accaaaagga aggtaagtcc gttttgacca ttgctatggg
     6901 atgtacgggt ggacaacacc gtagtgtggc atttgctaaa cgcttggcgc aggacttatc
     6961 caagaattgg tctgttaatg aagggcatcg cgacaaagac cgcagaaagg aaacggtaaa
     7021 ccgttcatga gaaaaccaaa gataacggtg attggtggag ggactggaat tcccgtcatt
     7081 ctaaaaagtc tgcgggaaaa agatgtggaa atcgcagcta tcgtgacggt ggcagatgat
     7141 ggtggttctt caggtgaact ccgaaaaaat atgcaacagt tgacaccgcc aggtgatctt
     7201 cgtaatgtcc ttgtggccat gtcggatatg cctaagtttt atgagaaggt ctttcagtat
     7261 cggttctctg aggatgccgg agcctttgct ggccatccat tgggaaatct catcattgct
     7321 ggcttgtcag aaatgcaggg ttcaacctat aatgccatgc agttattgag caaatttttc
     7381 catacaacag ggaaaattta tccttccagt gaccatcctt tgacccttca tgcagtcttt
     7441 caggatggga cagaagtggc tggagagagt catattgtag accatcgagg cataattgac
     7501 aatgtctatg tgaccaatgc cctaaacgat gatacgcctc tggccagccg tcgagtagtg
     7561 cagaccatcc ttgaaagtga catgattgtc ctagggccag gttccctctt tacctctatt
     7621 ttgcccaata tcgtgattaa ggaaattggg cgggctcttt tggaaactaa ggcagaaatt
     7681 gcctatgtct gcaatatcat gacccaacgt ggggagacgg aacactttac agatagcgac
     7741 cacgtggaag tcttgcatcg tcaccttggt cgccctttta tcgacactgt cttggtgaat
     7801 attgaaaaag tgcctcagga atacatgaat tccaaccgtt ttgatgaata cttagtgcaa
     7861 gtggaacacg attttgtagg tctttgtaag caagtttcgc gcgtgatttc atctaacttc
     7921 cttcgtctgg aaaatggcgg tgccttccac gatggagatt tgattgtgga cgagttgatg
     7981 cgcattatac aggtgaaaaa atgagtttca cagtagcagt aaaagaagaa atcctgggcc
     8041 aacaccatct gagctggcat gaattatctg ccattatcaa aatgtctggt agcatcggtc
     8101 tctcgacttc gggcttgact ttgtcagttg tgacagaaaa tgccaaactg gcccgtcacc
     8161 tctatgagtc ctttcttcat ttctatgaaa tcaaatctga aattcgtcac caccaacgga
     8221 gcaatcttcg caagaatcgc gtctataccg tttttacaga tgaaaaggtg caggatttgt
     8281 taagtgattt gcacttggca gactctttct ttggcctgga aacaggtatt gatgaggcga
     8341 ttttatcgga tgaggaagca ggtcgtgcct atctctgtgg cgctttcttg gcaaatggga
     8401 gcattcgtga ccctgagtca ggcaagtacc agttggaaat cagttctgtt tatctggacc
     8461 acgcgcaagg aattgcctcc cttctccaac agtttttact ggatgccaag gtgcttgagc
     8521 gcaagaaggg ggctgtgacc tatctccagc gagcagaaga cattatggac ttcttgatag
     8581 taatcggagc catgcaggca cgtgatgatt ttgagcgggt taagattttg cgagaaaccc
     8641 gtaacgacct caatcgggct aataatgccg agacagctaa tatcgctcgg acagtttctg
     8701 ccagcatgaa gaccattaac aatatcagta aaatcaaaga tatcatgggc ttagaaaatt
     8761 tgccagtaga tttgcaggaa gtggcgcaac tgaggattca gcacccagac tactctatcc
     8821 agcagttagc agatagtctc agcacccctc tgaccaaaag tggtgtcaat cacagactca
     8881 gaaaaatcaa taagatagcg gatgaattat aaagccacga atcctatgtg actagtggtt
     8941 cttttttata aactggtaga gtgttttggt tgtacttttt gttcagggtc aatgtagttg
     9001 atagcgttgt tgacagcagt tggagcttct ccgaggcctg tcgcaatcag atcaattttt
     9061 ccgtcatagt agcagcagtc accgatagca tagatacctg cttggctgga ttcctgtttg
     9121 ctgttgacga taatcttgtg acggttgagg ttgagccccc agtttttaag gttaccgaca
     9181 gaagatttga aaccatagtt gacaaagagg tggtctaggt caatggtttc agtttcatca
     9241 gatttgactt ttgtgatttc aagtttatca agtgtttttc catttccaag gagttggcta
     9301 ggggcgaatg gtgtcttgat ggttacagat gattcttgca aggcttgaac actgtgttcc
     9361 aaggcacgga aattatctct gcggtgaaca agggtagttg gtgcgatttt ttcaaaagcc
     9421 aaagcccaat ccacagccga gtctccccca ccaagaatcg tcactttctt accagcgtat
     9481 tgctgaatgt tagaaacgtg gtagtggata ttttcatagc cctcaacacc ttcaagttcc
     9541 agcggacgtg gtttgaaggc accgccaccc atagcgatga tgactgtttt agtcaggtga
     9601 cttcctttag aagttgtgat ggcaaattct tcttcttgtt tgtcaatctc aagaaccgtt
     9661 tcattgagat gaataggggt atcaaatcca tttagctgtt caatcaagcg gttagtcaac
     9721 tcttctccag tcaggtttgg gaagcctggt acgtctagga tttccttttc agggtagaga
     9781 atagcaggtt gtccacctag ctggggaaga gagtcgatga tttgaacctt ggcttggcgt
     9841 aggtgggcat aaaaggctgc aaaaagcccg acaggaccac cacccacaat ggtaatatca
     9901 tagagttgag acatagtttc tcctttgttt tttctagtca gtttatttta tcatattttt
     9961 gttcaatgta aaatggacta ggatagggaa aaaaatgcca gaaaatggta taataggacg
    10021 aaagtgtttt gatagggagg atatcaggga tgaattttca acaattatcc aatctgcaat
    10081 attggaccag tttgtttgca agtccatgga cgatagctat caatctgatt gatattttga
    10141 ttgttgctta tattttatac cattttacaa aagctattgc aggaaccaag attatgattt
    10201 tggtacgtgg agttttggtg tttattttag ctcaaatcct tgcaaatatg attggtttga
    10261 ctacgatttc ttggttaatc aatcaaatta ttacttatgg ggttattgcg gcggttgtta
    10321 tcttctctcc agagattcgg actggtttgg aacgtttggg aagagcgaca gatttctttt
    10381 ccaatgcccc tattagtgct gaggaacaga tgattcgtgc ctttgttaag tctgttgaat
    10441 acatgagtcc tcgtaaaatc ggggccttgg ttgctattca gcgtgtacgt accttgcagg
    10501 agtatatttc gacaggaatt cccttggatg ctaagatttc tgcagaactt ctcattaaca
    10561 tttttattcc caacactccc ctacatgatg gtgcggtgat tatcaaagaa gaacgtatcg
    10621 ctgtgacgtc tgcctatctg cccttgacaa aaaacacagg gatttccaag gaatttggga
    10681 ccagacaccg ggcggctatc ggtttatcag aagtctcaga tgccttgact tttgtcgtat
    10741 cagaggaaac gggaggaatt tcgataacct ataatggaag gtttaagcac aacctaacac
    10801 ttgatgaatt tgaaacagaa ttacgtgaaa tcttacttcc aaaagaggaa gtgggtctta
    10861 gttttaaaga acgattgcta ggaggatgga aacatgaaaa aaaatagttt atatatcata
    10921 tcctcactct tttttgcttg tgtcttattt gtctatgcta cggcgacgaa ttttcaaaac
    10981 agtaccagtg ctaggcaggt aaaaacggaa acctatacta atacagtaac aaatgtccct
    11041 attgacatac gctataatag tgataagtat tttattagcg gttttgcttc agaagtatca
    11101 gtggtcttga ctggtgcaaa tcgcctatcg ctagctagtg aaatgcaaga aagtacacgt
    11161 aaattcaagg ttactgctga cctaacagat gccggtgttg gaacgattga agttcctttg
    11221 agcattgaag atttacccaa tgggctgacc gctgtggcga ctccgcaaaa aattacagtc
    11281 aagattggta agaaggctca gaaggataag gtaaagattg taccagagat tgaccctagt
    11341 caaattgata gtcgggtaca aattgaaaat gtcatggtgt cagataaaga agtgtctatt
    11401 acgagtgacc aagagacatt ggatagaatt gataagatta tcgctgtctt gccaactagc
    11461 gaacgcataa caggtaatta cagtggttca gtacctttgc aggcaatcga tcgcaatggt
    11521 gttgtcttac cggcagttat cactccgttt gatacaataa tgaaggtgac tacaaaacca
    11581 gtagcaccaa gttcaagcac atcaaattca agtacaagca gttcatcgga gacatcttcg
    11641 tcaacgaaag caactagttc aaaaacgaat taaaaataga aaaggatttt ataacaatgg
    11701 gtaaatattt tgggactgat ggagtccgtg gagaagctaa cctagaacta acaccagaat
    11761 tagcctttaa actaggacgt tttggaggct atgttcttag tcaacatgaa acggaagcgc
    11821 cgaaagtctt tgtaggacgt gacacacgta tttcagggga aatgctggaa tcggccttgg
    11881 tggcaggtct cctttcagta gggattcacg tatacaaact tggtgtcctt gcaacaccag
    11941 cagtagctta cttggttgaa actgaaggag caagtgccgg tgtcatgatt tctgctagcc
    12001 acaacccagc ccttgataac ggaatcaagt tctttggcgg tgatggcttc aaactagatg
    12061 atgaaaaaga agcagaaatt gaagccttgc tagatgctga ggaagacact cttcctcgtc
    12121 caagtgcaga aggcttagga attttggtag attatccaga aggcttgcgt aagtatgaag
    12181 gataccttgt ttcaactgga actcctcttg atggaatgaa ggttgccttg gatacagcta
    12241 atggagcagc ttctaccagt gcccgtcaaa tctttgcaga ccttggtgcc caattgacgg
    12301 ttatcgggga aacaccagac ggtcttaaca tcaaccttaa tgttggttca acacatccag
    12361 aagcccttca agaagtggtc aaagaaagtg ggtcagctat tggtttggcc tttgatggag
    12421 acagtgaccg cttgattgct gttgatgaga atggtgacat cgtcgatggt gacaagatta
    12481 tgtacatcat cggaaaatac ctttctgaaa aaggacaatt ggctcaaaat acaattgtga
    12541 caactgttat gtctaacctt ggtttccaca aggccttgaa tcgcgaaggt attaacaagg
    12601 cagttactgc agttggtgac cgctacgttg ttgaagaaat gagaaaatca ggctacaacc
    12661 ttggtggtga acagtctggt cacgttatct tgatggatta caataccaca ggtgatggtc
    12721 aattatcagc agttcaattg actaaaatta tgaaggaaac tggtaagagc ttatcagagt
    12781 tggcggcaga agtaacgatt tatccacaaa aattagttaa tatccgagtg gaaaacgtca
    12841 tgaaggaaaa ggccatggaa gtgccagcta tcaaggccat catcgagaag atggaagaag
    12901 aaatggcggg gaacggccgt atccttgttc gtccaagtgg aacagagccc ctcttgcgtg
    12961 ttatggcaga agcgcctaca acagaagaag tggactacta tgttgatacc atcacagatg
    13021 tagttcgtgc tgaaattggg attgactaaa tcctagaaaa ctagatgaaa ataaaaaatt
    13081 atggtacaat agcttataat attgtagccg atggagaaag acatgaatct taaacgtgaa
    13141 caagaatttg ttagtcagta tcattttgat gcacgtaatt ttgaatggga aaatgaaaat
    13201 ggagctcctg aaaccaaggt ggatgtgaat ttccaattgc tccaacatga ccaagaaaat
    13261 caagtgactt ccttgattgt catcttgagc tttatgattg tatttgataa gtttgtcatt
    13321 agtggaacaa tctcacaagt aaaccatatc gatggccgta ttgtcaatga accaagtgaa
    13381 ttgaaccaag aagaggtgga aaccttggct cgtccatgtt tgaacatgct caatcgtttg
    13441 acttacgaag tgactgaaat tgcattagac ttaccaggaa ttaatttgga gttctaatat
    13501 gaaattagct gttttcacag attcctctgc ttatctcagt gaagagacct tgcaaagaga
    13561 agacttgttt gtcttggata ttcctgtcaa tattgatggt gaggaatatg tcgaaggcat
    13621 caatctgtct gctgaggaat tttaccaaaa aatggctcag gcttctgaat tgcctaagac
    13681 cagtcaacca agtattgcca agttagatga gatcttaact tcgctcaaag aacaaggcta
    13741 tacacatgcc ttggggcttt tcctatcttc tggaatttca ggtttttacc aaaatatcca
    13801 gtatatggtc gatgactatg agggcttaac cattgctttc ccagacactt tgattacaag
    13861 tgctccccta ggcatcatgg ttgaaagcgt ctttaattgg cgtgatcagg gcgatgattt
    13921 tgccagcatt caggataagc tagccattca aatcagccgt acgtcagcct ttatcatggt
    13981 agatgacttg gatcatttgg taaaaggtgg acgcctttca aatggggctg ccattttggg
    14041 caatttgctt agcattaagc caatccttta ttttaacgat caaggtgtga ttgaagttta
    14101 cgaaaaagtt cgtactgaaa agaaggccac caagcgctta attgaaatta tcaaggaaac
    14161 aacggcttca ggtcaatacc gggtcattgt cattcatggg aatgctcctg aaaaggctga
    14221 agaattgcgt cagcacttgc ttgattttgg cttgggttcg gatgtttcac ttgctacatt
    14281 tggtagtgtc attggaacgc acctaggagc aggaagtatt gctctgggtt atattccagt
    14341 gatttagttg gcgcttctag gctagttaag atgccttgta tcttagaaat tgaacacgga
    14401 ctacctgcct cttgaaaaaa gatgcctgtc ttgcctttcg tggaaagtca gtgccattcc
    14461 ctatttttca gaggcagcta aggtcctttg tatcttgata attgaacacg cctggaaccc
    14521 tgtgtgaaaa agatagttct tccaagaagt agacacttct tgattggaat tcctattttc
    14581 actttgtgtt cttacggact ttgtatctta gacaggagta gagatgagta ttcgagtaat
    14641 tattgccggt tttaagggaa agatgggcca ggctgcttgt cagatggtat tgactgatcc
    14701 agacttggac ttggtggcag ttttggatcc ttttgagtct gagtcagaat ggcagggtat
    14761 tcctgttttc aaggataagg ctgatttagc tggttttgaa gcggatgtct gggtagattt
    14821 tactactcca gctgttgcct acgaaaatac acgttttgct cttgaaaatg gctttgctcc
    14881 agtagttgga acgactggtt tcacgagtga agaaattgca gagctaaaag aattttctcg
    14941 tgcccaagac ttgggtggcc tgattgcccc taactttgcc ttgggtgctg tcttactcat
    15001 gcaatttgcg acgcaggctg ccaaatattt cccaaatgtg gagattattg agctccatca
    15061 tgacaagaaa aaggatgctc cgagtggaac agccattaaa acagctgagt tgatggcaga
    15121 ggttcgagag tccattcagc aaggcgcagc agatgaggaa gagctgattg ctggtgctcg
    15181 tggtgctgac tttgatggta tgcgcatcca ctcagttcgt ttgccaggct tggtagctca
    15241 tcaggaagtc atctttggca atcagggaga agggctgacc ctccgtcatg actcctatga
    15301 tcgcatctcc ttcatgacag gagtcaattt gggaattaaa gaagttgtca agcgtcatga
    15361 gcttgtctat ggattagaac acttattatg agattaacgc aaatgccttc tgaatttcag
    15421 aaggctttac cagtattaga aaaaattaaa gaagcaggct ttgaggctta ttttgttggg
    15481 ggctctgttc gagatgccct tctccatagc cctatccacg atgtggatat tgcgacgtct
    15541 tcttatccag aagagaccaa gcagattttt ccgcgaacag ccgatatcgg aatcgagcat
    15601 ggaaccgtct tggtcttaga tggggatgag gagtatgagg taacaacctt tcggacagag
    15661 gatgtctatg tggactatcg cagacccagt gcggtttcct ttgtacgatc gctagaagaa
    15721 gacctcaaac gccgtgattt cacagtcaac gcctttgcct tggatgagac aggagaaatc
    15781 gttgacttgt tccatggttt agaagatttg gaaaagcaag tcttgcgagc agttggagtg
    15841 gctagtgagc gtttcaacga agatgcttta cggattatgc gtggtttccg ttttcaggct
    15901 agtcttggtt ttgcacttga gccagaaaca tttaaagcta tgaagacctt gacgccgctt
    15961 ttggagaaaa tttctgtaga gcgtaccttc gtcgagtttg ataaactctt gctggctcca
    16021 ttttggagaa ggggcttggc ttccatgatt gagagtcaag cttatgacta tctccctgat
    16081 atggcatcta gccaggacaa gctcaacaga ctgtttgatt tggagactga ttttactttt
    16141 gaatcctctg aacaagcctg ggcggcttta ctatgggctt tggagattga aaatgcgcag
    16201 tcacttttga aatcttggaa gacctcacgc cagtttgcca agcaagttca ggatttgctg
    16261 attattttgg ctctgcgtga aaatggagaa ttgagcaagc gagattgtta tcgctttgac
    16321 atagatttgc ttttacaggc tgaaaatctt cgtcaagctc aaggaaaaga agtcaaccca
    16381 caagccatca cagaaaaata ccaaagcttg actatccatg acaagaaaga gattcagatt
    16441 aatggcggta ttttgatcaa ggaatatggc tatcagccag gcccagactt gggagagatt
    16501 ttaacagaga ttgagtttgc cattgtcgat ggagaattgg agaataatcg tgaagccatc
    16561 catgcttacc tgagggagaa aaaatgagtg attttatcgt tgaaaaacta agtaaatccg
    16621 ttggtgacaa gaccgttttt agggatattt cctttattat ccatgactta gacagaattg
    16681 gtttaatcgg tgtcaatggg actggcaaga ccaccctttt ggacgtcctt tctggtgttt
    16741 ctggatttga tggggatgtc agtccttttt cagctaaaaa tgattaccag attggttact
    16801 tgactcagga tcctgatttt gatgatagaa agacagtttt ggatacggtt ctatctagtg
    16861 aactcaagga aatccagctc attcgtgagt atgaattgat tatgctcgac tatagtgagg
    16921 acaagcaggc gcgtttggaa cgtgtcatgg cagagatgga ctctctccaa gcttgggaaa
    16981 tcgaaagtca ggtcaagacc gttcttagca aattgggcat tcaagactta tctactcctg
    17041 ttggggaatt gtcaggtggt ctgagaagac gggtacagtt ggcacaagtc ttacttggca
    17101 accacgacct cttgcttttg gatgagccga ccaaccatct ggatattgcg attattgagt
    17161 ggctgaccct ctttttgaaa aattctaaga agaccgtcct ttttatcact cacgatcgtt
    17221 atttcttaga cgctttgtca acacggattt tcgagttgga tcgtgcaggc ttgaccgagt
    17281 accagggaaa ttaccaggac tatgttcgcc taaaggcgga acaggatgag cgcgacgcgg
    17341 ctcttcttca caaaaaagaa caactctaca aacaagaatt ggcctggatg cgcagacaac
    17401 cgcaggcgcg tgcgaccaag cagcaggccc gtatcaatcg attccatgat ctgaaaaagg
    17461 aagtttcagg cagtagtgct gagacagact tgaccatgaa ctttgaaacc agtcggattg
    17521 ggaagaaagt catcgagttt caggctgttt cctttgccta tgaaaacaag cctattttgc
    17581 aagattttaa tctcttggtg caggccaaag accgtatcgg aatcgttggg gacaatggtg
    17641 ttggaaaatc aaccctgctc aacttgattg caggaagtct tgagccaact aaaggacaag
    17701 ttgtgattgg ggaaactgtt cgcatcgcct atttctctca gcaaattgag ggtttggatg
    17761 aaagcaagag agttatcaat tacctgcagg aagtggcaga agaggtcaag accagtggcg
    17821 gttctacgac ttcgattgca gagttgctgg agcagttcct tttcccacgt tcgacgcatg
    17881 gaaccttgat tgagaaattg tcagggggtg agaaaaaacg tctttatctc ctcaaactgc
    17941 ttttggaaaa accaaatgtt cttcttttag acgagccaac caatgaccta gatattgcaa
    18001 ctttgacagt cttagagaat ttcttgcaag gttttgcagg tcccgtttta acagtcagtc
    18061 acgaccgcta tttcttggat aaggtagcga ccaagattct cgcttttgag gatggcaaga
    18121 ttcgtccttt ctttggtcat tacaccgact atcttgatga aaaagctttt gaaacagata
    18181 tggccaatca agtgcaaaag gtcgaaaagg aaaaagtggt caaggttcga gaagacaaga
    18241 aacgcatgac ctaccaagaa aagcaggagt gggcaagtat tgaaggtgat attgaaacct
    18301 tggaaaaacg tatcgctgct attgaagagg aaatgcaggc taacggctct gactttggta
    18361 agctggctac tctccaaaaa gaattggatg agaaaaatga agcactcctt gaaaaatacg
    18421 aacgctatga gtatctcagt gaatttgata gttaagacta ttcgaaaatc aaagtgcaaa
    18481 ctatgtcggc ttcacattgc cgactcaagt acagcctgtg accggcttcc taatttgcac
    18541 tttgattttt attgagtata aaagaataga aaaatcccgt ctcatgaaag gatttctcta
    18601 ttcttttttg gtttcttgat gaaagatatt ttgccaggtt tcgtggcgac gccagatact
    18661 ggtatggatg ataccatcta actcatagca gatgagtttg gttttttgac tgatggaagt
    18721 aatttgaatg tcctttatag cagaatttaa ctcttttttg gtcttataag cctctttatc
    18781 catctgctct ccatcttggc gaatatagac aaaatcatca gtcaagagtt cttctagttg
    18841 gtttccttgg tcaatcaatt gttcacgcat aagcaatttt ttatagacat tgtctaaaat
    18901 ttcatcctca gggataggtg ctggttcgat atggacatcg gtatcaaaga cgccaaaacg
    18961 ctcctccagc atagactcga cctgatccgc gatttcatgg ctttcaaaaa cagacaagtc
    19021 aggattcatc tctagtgtaa tatccaggta gatgttgcta ccgtaggtgc gacctctttg
    19081 cgatttgacc ttgctgattt tgggaatttc catgatagcc ttttggtagt cctcgagcag
    19141 gcggtcgtca aagccatctg aaagactaaa ggaagactcg atgaagatat cataggcagt
    19201 cttcaagata aagaaagtga tgatgatagc aaccagttta tccacaatcg gataattgaa
    19261 actactagct aggatggcaa tggcggttcc aagtgaggta acagcgtcag aaagattgtc
    19321 cttagcagct gccttcagcg ccttggagtt ggatttctta ctgaggcgag tattgtagag
    19381 atagaccaca aacataatcg ctgcagaaat gattcctaga gttgcaccaa gaggatcaat
    19441 gaccgtttct tcccgactga gaatcttttg aatggtatct cttagaacat cgaaaccgac
    19501 atagaacatg atgatagaag tgatcaagct tgccaaatct tcaatcttcc aatgaccaaa
    19561 acggtggtca cggtctgcag gctggcgcgc catccgaatc ccgattaaga gggccacatt
    19621 tccaatgatg tccgatacgt tattaaaacc atcggccacc aaactggatg aatgaaggag
    19681 atgaccagct gctaatttag ctgcagacaa gatcaaatag gtcgaaatac tgataatggc
    19741 tccacgctca gctaacttga gatttgagat agattgcttc attcaacttc ctttctagtc
    19801 acatagcatt ctaccattat actggttttc aaggaaaaat tcaaataagg cagtttttac
    19861 tctttgaaaa ctccccaaaa tttggtataa tggtactact caagggagta gctggcagaa
    19921 acctgtgata gtgtcgtcat tccgaatttt atgctgaaaa gtatgctttc cggccctatc
    19981 ttaaacagcg agacttgtta tgattaacag gtctcttttt gtttgtcgtg aaaagatatg
    20041 acagaaagag agtctgtttt gcacacaatg tcaaggagga gacacatgtc aaaagaacaa
    20101 aaacgccaag cgttttatac tcagagccct gaagaggtct tgcaggctgt ggatgcgacc
    20161 gagcaaggtt tgtcatcaag tgaggcggaa aagcgccttg ccgaatttgg gcacaatgaa
    20221 ctcgaagaag gcgagaaacg atcaatcttg gtcaaattca tcgagcaatt taaggatttg
    20281 atgattatca tcctagttgc ggcagcaatc ttgtcagtcg tgacttctgg tggggaagat
    20341 atcgcagatg ccattatcat cctagctgtg gttatcatca acgctgcctt tggtgtttac
    20401 caagaaggaa aagctgaaga agctattgaa gccctcaaat ccatgtctag tccagttgcc
    20461 cgcgttcttc gtgatggaca catggcagag attgactcta aagaattggt accaggcgat
    20521 atcgttgccc ttgaagcagg tgacgtggta ccagcggacc tacgtttgat agaagccaac
    20581 tctcttaaaa ttgaagaagc agccttgaca ggtgaatctg taccagtcga aaaagacttg
    20641 tcagtcgagc ttgcgacaga tgctggtatt ggtgaccgtg tcaacatggc cttccaaaac
    20701 tcaaacgtaa cctatggtcg tgggatgggt gttgttgtca atacaggtat gtacactgaa
    20761 gttggtcata ttgctggtat gcttcaagat gcggatgaga ctgatacacc actcaaacaa
    20821 aacttgaaca acctttctaa ggtcttgacc tatgctatct tggtcattgc ccttgttact
    20881 tttgtagtgg gtgtcttcat tcaagggaaa aatccacttg gtgagttgtt gacttctgtt
    20941 gcccttgccg ttgcagccat tccagaagga cttcctgcta tcgttaccat cgttctttct
    21001 cttggtactc aagttttggc caaacgacat tccatcgttc gtaagttgcc agcagttgaa
    21061 acacttggtt caactgaaat catcgcttct gataagactg gtacgctgac tatgaacaag
    21121 atgacagtcg aaaaagtctt ctacgatgcg gttctacatg actcagctga tgatattgaa
    21181 ctaggtcttg aaatgccact acttcgttca gttgtcttgg ccaatgatac gaaaatcgat
    21241 gtggaaggta acttgattgg tgacccaacc gaaacagcct ttatccaata tgccttggac
    21301 aagggctatg atgtcaaagg tttcttagag aaatatcctc gtgtagctga attgccattt
    21361 gactctgacc gtaagctcat gtcaacagtt cacccattgc cagatggtcg tttccttgta
    21421 gcagtcaagg gtgcgccaga ccaactctta aaacgttgtc ttcttcgtga taaggctggg
    21481 gatattgctc cgattgatga gaaggttaca aatctcattc atacaaacaa ttctgaaatg
    21541 gctcatcaag ccttgcgtgt ccttgcaggt gcttataaga ttatcgatag tattccagaa
    21601 aatctcactt ctgaagagct tgaaaatgat ttaattttta ctggtttgat tgggatgatt
    21661 gaccctgaac gtcctgaagc cgctgaggct gttcgtgtgg ctaaggaagc gggaatccgt
    21721 ccaattatga ttacaggtga ccaccaagac acagcggaag ccattgccaa acgtttggga
    21781 atcattgacg caaacgatac agaaggtcac gttttaactg gtgctgaact caatgaactg
    21841 tcagatgaag aatttgaaaa agtcgttggt caatactctg tttatgcccg tgtgtctcca
    21901 gaacacaagg ttcgtatcgt caaggcttgg caaaaacaag gtaaagtcgt tgccatgaca
    21961 ggtgacggtg tcaatgacgc gccagctctg aaaacagccg atatcggtat cggtatggga
    22021 atcactggta cagaggtttc taagggggct tctgatatga ttcttgcaga tgataacttt
    22081 gcgactatta tcgtcgcagt ggaagaagga cgtaaggtct tctcaaacat tcaaaagact
    22141 attcagtacc tactttctgc taatactgct gaagtattaa ccatcttcct atcaaccttg
    22201 tttggttggg atgtcttaca gccggttcat cttttgtgga tcaacttggt aacggatacc
    22261 ttcccagcta tcgctcttgg tgttgaacct gcagaacctg gtgtcatgaa tcataaacca
    22321 cgtggacgca aggcaagctt cttctcaggt ggtgttttga gttctatcat ttatcaaggt
    22381 gtactccaag cagctcttgt tatgagtgtt tatggccttg cgattgctta cccagttcat
    22441 gtgggtgaca atcatgctat tcatgcagat gccctaacga tggcctttgc aacccttggt
    22501 ttgattcagc tcttccatgc ctacaatgtc aagtctgtct accaatccat cttgacagtt
    22561 ggcccattca agtctaagac ctttaactgg tccatcttgg tatcctttat ccttctcatg
    22621 gcaacaatcg ttgtagaacc gcttgaagga atcttccacg taaccaaact agacttgtca
    22681 caatggggaa ttgttatggc tggaagcttc tcaatgatta tcatcgtcga aatcgttaag
    22741 tttattcaac gcaaactcgg ttttgacaag aatgcgattt aagggaggtt tcggatgtca
    22801 gcatatcaat taccgaccgt atggcaggat gaagctagta atcaaggagc ttttacgggg
    22861 ctaaacagac caacagcagg tgcccgtttc gaacaaaact tgccaaaagg agaacaagct
    22921 tttcagcttt attcactggg aacaccaaat ggtgtgaagg ttactatctt attggaagaa
    22981 ttactagaag ctggttttaa ggaagcggct tacgacttgt ataagattgc tatcatggat
    23041 ggggatcaat tcggctcaga ctttgtgaag ctcaatccaa attccaagat tccagcctta
    23101 ttggaccagt caggtactga aaacgtaaga gtctttgagt ctgctcatat tcttctttac
    23161 cttgctgaga aatttggagc ctttttacca agtaatcctg tggaaaaggt agaagttttg
    23221 aattggctat tctggcaagc aggtgcagca ccttttctag gtgggggatt tggacatttc
    23281 ttcaattatg ctcctgaaaa attggaatat cctattaacc gttttacgat ggaagtgaaa
    23341 cgccagttgg atttattgga taaggaattg gctcagaaac cttatattgc aggcaatgac
    23401 tatacgattg cagatattgc tatctggtct tggtatggac agttagttca aggaaatctt
    23461 taccaaggtt ctgcaaaatt cttggatgcc tcaagttatc aaaatctagt aaaatgggca
    23521 gaaaaaattg ccaatcgtcc agctgttaag cgtggattgg aagtaactta tacagaaatt
    23581 aaatagcaaa aaagtcatct tcggatggct ttttgctaca gttgagggaa agggtttgca
    23641 gttatcggct tttgtgctat aattgctata atatagtaaa aagcaagagg agtgtgagga
    23701 agtggaaaag aaaattgtga aggatatctt atttttatct caagtgtctc agccggcaag
    23761 tcaggaggac ctttatcttg ccagagattt gcaggataca ctcttagcaa atcgtgatac
    23821 ctgtgttggt ctagctgcca atatgattgg ggtgcagaag cgcgtgatta tctttaatct
    23881 tggcttggtt cccgtggtca tgtttaaccc agtgcttctg tcctttgaag gatcttatga
    23941 ggcagaagaa ggctgtttgt ccttggtagg tgtgagatca actaagcgtt atgaaaccat
    24001 aaggcttgcc tatcgtgaca gcaagtggca ggaacagacc attaccttga caggcttccc
    24061 agctcagatt tgccagcatg agctggatca cttggaagga cgaatcattt aggaggaaag
    24121 caaatgaaac gaatagtctt tgaacttatt tttatcgcaa cgacctggta tatcttttta
    24181 ccgcccctta acctgaccag ctgggaattt ctcttcttcc tctgtgggca tttgttagtt
    24241 gtggcaatct tatttggctt tggcaagggg ataaaccttg tcaaaacggt tcatgtgcgc
    24301 cacggtaagg cggaagctgc cttaaatctt gagggtttca aaatcaatcg gttagggaaa
    24361 attctgttag cttcgattgg aggaattctt ctcttggcag ctttggtttc cttggtaact
    24421 tccagcatgt ttcaggctaa aaattatgcc aatgtagtca cagttacgga aaaagacttt
    24481 actgaatttc ctaagagtga caccagtaag gttcctatcc tagatagaag tactgctgaa
    24541 aaaattggag accgctactt gggttcccta accgataagg tgtcgcaata cgtagcggca
    24601 gatacctata cccaattgac aattgatggg aaaccttatc gggtcacacc actagaatat
    24661 gcagacccta tcaaatggtt taacaatcaa gccaagggaa tcggtgagta tattaaggtg
    24721 gacatggtaa ctggaaatgc ggatttggtg gacttgaaga caccaatcaa gtattcagac
    24781 tcggagtatt ttaaccgtga tgtcaaacgt cacctgcgct tgaagtaccc gaccaaaatc
    24841 tttaaaactc catcttttga ggtggacgat gagggcaatc ctttctatgt agcaacggtt
    24901 taccaaaagc aatttggact tgctgttcct cgtcctgctt cagtcattat cttggatgct
    24961 acaaatggag aaaccaagga atacagctta tcagatgttc cagaatgggt ggacaggatc
    25021 tatccagcag aggaaaccat tgagcaaatc aactacaacg gcaagtacaa ggacggtttc
    25081 ttgaatgcca tgatttccaa gaaaaacgtg acccagacta ccaatggcta taattacttg
    25141 tctatcggta atgacatcta tctctacaca ggtgtgacgt cggctaatgc ggatgagagt
    25201 aatcttggtt tcatccttga aaatatgcga acaggagaaa tcactaagta tagcttggct
    25261 tctgcgacag aagaatcagc ccgtgaatca gcagaaggtg ctgttcagga gaaatcctac
    25321 aaagcaacct tcccaatcct catcaacctc aatgacaagc ctctctacat catgggcttg
    25381 aaggacaatg ctggcttggt caaagagtac gccctggtag acgcagtcga gtaccaaaat
    25441 gttatcgttg ctactacagt ggaagagatg ctcagcaagt atgccaataa aaacgacctt
    25501 gaaattgaca atgcaacgac agaaagcatc aagggagtag tagcagacct caaatcagct
    25561 gttatcaagg gagacactgt ctacttcttt aaagttgatg gcaagatata caaggtcaag
    25621 gcttcagtat ccgatgacct tccttacctt gaaaatggta aaaccttcga aggtcaagta
    25681 ggaaaagaca attatctcaa gacctttaag ctacggtaaa aataggtttt ttcagaaagt
    25741 atatgttata atagagtaaa ttaagactaa tggaggacaa agaaatgggt ttttatttga
    25801 tggttgcttc aatgctactc ggactgctcg ctttgaagat aggtttttct cagttcaagg
    25861 agaaaaaaga taaattctta tctatcttaa caagtttagc tggcacagcc cttgttctcg
    25921 tggctgtctg gttaggatgg cccaaataaa tagaaaaacc tcggtcaaac cgaggttttt
    25981 caaatgaatt tcttggttgt ggctaaaaaa tatgctacac tatcaatatg aaaattttaa
    26041 tcccaacagc aaaagaaatg aacacagact tgccaagtat cgaggcaatt cctttaaaac
    26101 cagaaagtca gaccgtgctt gatgccttgg ctctctattc tgccagtcaa ttggagagtt
    26161 tctacaaggt atcagctgag aaagcggcgg aagaatttca aaatatccaa gctttgaaaa
    26221 ggcaaactgc tcaacactat ccagccttga aactttttga tgggcttatg taccgcaaca
    26281 ttaagagaga taagctgacc gaggcggaac aagattatct tgaaaatcat gtcttcatta
    26341 cctcggcttt gtacggtgtt gttccagcct tgtcacccat ggctcctcac cgtttggatt
    26401 ttttgatgaa attaaaagtc gctggtaaga ctttgaagag ccattggaag gcagtctatg
    26461 atgaagctct gaagaaggaa gaagtgattt tctctctctt gtcatcagag tttgagactg
    26521 tattttctaa ggaaatcaga gcaaagatgg tgaccttcaa attcatggag gatagaggcg
    26581 gtcagctgaa gattcactca actatctcca agaaagcgcg cggggccttt ctaacagctt
    26641 taatagaaaa tcaagtacaa actgtggggg aagcacgtcg cttgaacttt gctggatttg
    26701 tttaccgaga agatttgtca caaccacagg gattggtttt tgtaaaggaa gtatagaaat
    26761 cagataagag aaaagtcagc acgattatgc tgactttttt gataacttat aaagtattgg
    26821 acaaatgaat ctcatttgat aaggtgttaa gatgattaac caaatttatg attgacagat
    26881 gatttcggca aataaacttc ttggtaatct ctggaatata gaaattagac acgatgatat
    26941 cgtaatctgt ctgatttaaa atctcaggac ttgtttttaa ttcatcccaa atatcgaagg
    27001 taaagcggtt attacagtaa taggaaagca tatcaacaaa ggtaagggat atagcatggt
    27061 caaagttact gataatcaaa accttgatag gtggtcgatt ttctaacaac tgagtagaga
    27121 ggttttcagc atgggttaag atggtgtaca tcaagtgttc caactgttca ggatggtcat
    27181 gctgtccaat tgcctgacgg tattgagcca attcttgacg cgcacttccc ataaagtctg
    27241 ggaagtaaac ttcaaatttt ttaatcgtca atgctttttg ttcaaataag attggggtag
    27301 aaaagatttc ctgtctttca aagaaagcag tgttgtgtaa atgccaaatc agctcatcgt
    27361 ggttagtaaa agtaatctta tattgttttg ataggcgttc taatatttga ctaagtaatt
    27421 ggtaagagta tcttgcttga ctgttatctt ctaaagagtt gaagaattct tgaggatcta
    27481 ggaaaatatc attctgaaga aaagaaataa aaatttgagc gattgtatct ggagtcattt
    27541 caagcccaaa atgtttagag aaataagcaa gtgtctcctg gaaatttgga atgctcttta
    27601 aaagcggata atattctttg taaaagtggt tggggaggtc aatgaagtga ccatttttaa
    27661 ccctgtggat actgatagct atcatcagtt tgtacattct ataaattgaa aatcgcattg
    27721 gataattcgt gattttatag aagaagtcag caaactctgt taaatcttcc tcaggaaggt
    27781 caggaaaagg ccaatctagg aaataatagc gctcagaaaa gtattgggca aaaaagtaac
    27841 gaatatcgat ctcatttcca cgtatctcgg atggagtaaa agataattca atttgaaact
    27901 gagatgaaag aacctttgta atatttctgc ccagtcgata gaggttggca ctactaaagt
    27961 aaagattttc tattgttcga taaataggta gcccctcatt gaagaacata tactctagta
    28021 attgaaaaga ttgagaatta gcaagaaaat attgataaat atcttctaca ctagtgttaa
    28081 cttctaaatc tatcatgatt ccgttaacag aagattggat gttaatagat ggaaaggcaa
    28141 tacgtaattc atttaaatca ctttttaaga ttcgttctgt gcatcctaat ttttcggcta
    28201 gttcatgtaa tttcatccaa ttacgattct gaattaggtt ttccattaag cgtaattgtc
    28261 tttgaacttt tgtggataaa aggtttctca tacgggtatt ttctccataa ttcattatat
    28321 ctttttttgt ctaagtagta taaaaaaatt agagcaagaa aattgtcgtt agtaatcgga
    28381 aaaattagaa taatataagt ggcttttaag tgcaaaaaca gaatatatta attgttcaga
    28441 aaatattgat taatgcctca aatagttgta cattgtaaac tataaaatac tatctaggag
    28501 tttaaaatga aacattcaca taaaaaatca tttgactggt atagcatgca acaacgttat
    28561 tctattcgta agtattactt tggtgcagtt agtgtcttgc tcggtaccgc tttggtatta
    28621 ggtgcagcag ctagtgtcca aacagtacaa gcgaaagaaa acaaacaaga aactaccaat
    28681 agtatttctg ttggtagggg agaagcagct actaaactag cagaggtttc tgcgtctaat
    28741 aaagagaaaa cctatgcagc tccaactgta gctaatccag tagaaacgac tccagttaaa
    28801 actggagaag ttactaaacc agcagaaaaa gttgaagaag caaaagacaa aaaagaggaa
    28861 gtaacgcatc aagatgctat tgacaagtca aaattgttaa cggctctttc gcgtgctaaa
    28921 aagttagaaa gcaagttata tacagaagca agtgctgcta acttgcaaac aagtatccaa
    28981 gctggtcaaa gcttgcttgg gaaagcagat gcatctgaag ctgaattatc agcagcagag
    29041 tcatctattc aatcatctat tattggtcta gaacttcgtt ctaactctaa caaaggaact
    29101 gtatcagaaa ctcctgtagc gaagaaagct aatatagttg aagcgaagga agagactaat
    29161 ccagctgcaa caactgatcg ttcagctgtt gatagtgctg ttttgccaac tagcacagct
    29221 gccaaagtag aaacaacttc agctccagca tctattaatg aaatcttgaa accaggtttg
    29281 agcctttctg atgctcgcca aaatccagct atccgtaaag aagacttgga taaggggtat
    29341 agtggtttta gagcagcacc tgctccaacg aacaaactag taactaatct aggaaataat
    29401 actgttttca ctgatattag cagagggtct catactttcc gtggtaatgg gaattcacgt
    29461 ggtggaaacc cgattcattt tgatgtaaca acaactagag ttggaaatag agtagaattc
    29521 tcaattgcat attctggtcc aggtgagttc gttaataata actttctctt agacaaaggg
    29581 aatggatttg gagaaccatc tcgtgcaact atttcaacaa acagattaag agatcaaact
    29641 aaagatataa gaaagggtgc aaattttgta tctcattcaa gctacagttt aacatctgct
    29701 gttgaaacaa acagtaatca aactattaga tttagtttac ctatagccaa ccctaatggt
    29761 gatctgtctg ttcgattaag acctgttact tttaatgtag gtcaagggga atcaggatct
    29821 gcaactagta atgatccata tagtaactct aactattact tcagagaaaa accactcctc
    29881 ttggatgcaa atccaaatgg tggtactaat aataagactg tttcagaaga cattgacttc
    29941 caaactgtct atcttccaac tagtaagtta ccagaaggtc aaactagatt agttcgagaa
    30001 ggtgaaaaag gacaacgtca aatcatctat aaagttcatc aattgggtaa cgaaacaatc
    30061 ctaggattgc caattagtaa ttcagttatt aaagaagtta agccacgtat tatgcaaatt
    30121 ggtgtggcta aagatctaat cgatacagta aaaccacgtg ttgatcaaaa taaagtcggt
    30181 gatacaaata acctcacttt ctatcttgat aacgatggaa acggtgttta tactgagggc
    30241 gtagacgaac ttgttcaaaa aattgctatt aaagatggtg ctaaaggtga aaaaggagtc
    30301 caaggcgaac gcggcctgac tggagcgcaa ggtcaggcag gtcgtgacgg tgtaactcca
    30361 accgtaaccg ttaaagataa taaaaatgac ggcactcata ctatcactat taacgacggt
    30421 agaggtaatg ttgcaagtac tgttgtaaga gatggtttcg atggcgcaag tccattagtt
    30481 gcgactcaac gaaatgaagc agataagaca acaactgtta tcttctatta tgatcaaaat
    30541 ggaaataatg aattagatgc ttctgataag aaattaaaag aagttattat tgcagatggt
    30601 gccaaaggtg aacaaggtct tcaaggtcgt gatggagctc aaggaccaaa aggagaacaa
    30661 ggtaatccag gaactccagg taaagatgga aaatctctaa ttgctgttaa aaatggaact
    30721 gaaacaaaag tatatgtaga ggatccggct agaccaggac aaccacttaa tccaaaccaa
    30781 ccattagcaa ctatcactga tggaagaaat ggtactaatg gacaatcacc aacaattaca
    30841 gcgacacgaa gtgttcaaaa tggtaaaaat ggtgtattag taacaattac accagtagga
    30901 ggtcgtccac aaacaacatt tgtagaggat ggacaaaaag gtgctgatgg gaaaacccca
    30961 acagtaacaa taactgaggg gcaaaacggc acacatacat taacggttca taatccagga
    31021 agtccagatg tgacaactac gatccgtgat ggagctacag gacaagcagg tcgtgatggt
    31081 aaagatgtat taaacggaaa agtaaatcca caaccaaacc aaggtaaaaa tggagataaa
    31141 tatattaata ccgaaaccgg tgatgtctat gttaaaaaca atggaaactg ggataaagaa
    31201 ggcaacatca aaggccctaa aggggacaaa ggtgcagatg gtgctaaagg cgaaaaagga
    31261 gaccaaggcg aacgcggcct aactggagcg caaggagcta aaggtgcgga tggcgcagta
    31321 ggtcgtgatg gacgtgacgg taaagacgtg ttgaacggca aagctaaccc agaagcacat
    31381 caaggtaaag acggcgataa atacgttaat acagaaacag gcgacgtctt cgttaagaat
    31441 aacggcaact gggataaaga gggcaacatc aaaggcccta agggtgacaa aggtgaacgc
    31501 ggagaagatg gtaagactcc agaagtaact gtaactccag gtaaagatgg ccatagtact
    31561 gacattacat tcactgttcc aggtaaagat ccagttacag taaatgttaa ggacggagaa
    31621 aatggtctga acggtaaaac tccaaaagtt gatttacttc gtgtccaagg aaaaaacgga
    31681 aatccatctc atacaattgt gacattctat acagatgaaa acaatgacgg caaatataca
    31741 ccaggaactg atgaacttct aggttcagaa atgattaaag atggtgctaa aggcgcggac
    31801 ggacgagatg gtaaatcatt gcttactgtc aaggatggta aagaaactaa agtttaccaa
    31861 gaagatccag ctaacccagg acaaccatta aatccagaaa aaccacttgc ggtaattaga
    31921 gatggagtag atggaaaatc acctacagtt acagctgttc gtaaagatga agcagggcat
    31981 aaaggtgtag aaatcactgt tgataaccat gatggttcac aaccaactac agtctttgtt
    32041 caagatggtg ctaagggaga aactggtgca atcggtcagg atggacaaac tcctacaatc
    32101 actactcaac gtggacaaga tggccaaagc actgttgtaa ctatcacaac accaggtaaa
    32161 gatccagtaa ccttcactgt aaaagatggt aagaatggta aagatggccg tgcaccgaaa
    32221 atcaaagtag aagatattac ttcaccttca agaattagac gcgctacaga tgctgctgca
    32281 actccaacgc gtaacggtat ccgtgttaca gtttatgatg atgttaatga caatggggta
    32341 tacgacggag gtgtcgataa agtattaaat agtaaagata tttataacgg tatagatgga
    32401 cgtgatggtt cagctccaac tattactaca aaagataatg gagatggaac acacactatc
    32461 acagttcaaa atcctgatgg ttctgaatca acaactgttg ttaaagatgg taaagacggt
    32521 aaaactgcga atatcactac aacagaaaac ccagatggaa gccacacaat tacggtgaca
    32581 aatccagatg gttcaactaa agaaactgtt gttaaaaacg gtaaagacgg taagactcct
    32641 aaagttgaag taacggataa caacgatgga actcacacag ttaaagtgac ggatggagac
    32701 ggtaatgtaa cgaacgctat catcaaagat ggtaaagatg gtaaagctgc aacagcaaca
    32761 actactgaaa atccagatgg aagccacaca gtaacaatca ctaacccaga cggaactaag
    32821 aatgagtttg ttgttaagaa tggacgtgac ggtgttgacg gacgtactcc aaccgcatct
    32881 gttcgtgata atggagacgg aagtcataca atcgttatta caaatccaga aggtgtgaca
    32941 actgaaacca cagttcgtga tggtaaatca ccaaaagtga ctataactga tgaacaaaat
    33001 ggaactcata agatctctgt tctaaatggt gacggaacaa ctactgaaac aatcattaaa
    33061 gatggtaaat caccagtagc aacagttaga gataaccaag atggtactta cactattcgt
    33121 gtggaaaatg gtaatggtac tgtttctgaa accacagttc gtgacggtaa atcaccaact
    33181 gctaaggttg tggataacgg agatggaaca cacactatca cagttgtgaa ctcagacgga
    33241 acaactacaa caactacagt tcgttatggt agagaaccaa aacttgaagt tattgataac
    33301 aacgatggtt cacacactat taaagtgaca ggtgctgatg gtaaagaaac gacaactaca
    33361 atctttgatg gtaaatcacc aaaagcgaac atcgttgata acggagatgg aactcataca
    33421 ttaacaatcg tagattctga tggtcgtgaa tacaaatcta ttattaaaga tggtaaagac
    33481 ggcaaagatg gcgtttcacc aactgtaact gttgaaaata ataacgatgg aactcacgtt
    33541 gttacaatca ctaatccaga tggaagtaag acagaaatgg tgattaaaga cggtaaagat
    33601 ggtaaatcac caaaagtttc tgttgaagat aatggtgatg gtagtcatac aatcacaatc
    33661 atcaattctg atggaactgt gacaaaaaca gttattaaag atggcaaaga tggtagagat
    33721 ggacgtgatg gtagagacgg caaagatgga aaatgtggat gccaagacaa accagtaaca
    33781 ccatcaaatg acaaaccagt acctccagca ccaaatgtgc cgacaccaga agtactggtt
    33841 aaacctgtgc cagcgcaacc aacaccaaat gtaccgacac cagaagtgcc agtacaacca
    33901 actccagctg ttccaacacc agaagtaccg gttccagaac aaccagtagt accaacaccg
    33961 gctcaaccag caactccagt aaatgctaat ccagtagcac caactacagg taaagaaaac
    34021 cgtggggata aattacctga aactggaagc caatctgatt atatctctgt tcttttaggt
    34081 agcggtattc tattgagcct atatgtagga cgaagaaaag aagattaatc agccaaaagc
    34141 agtcactaat ttctcgaaag aagatgataa gtgatagatc tcgaatgaaa gaatattctt
    34201 agaatgattt tcctatgatg gaaaagactg ctaaaaacct tgagtggtaa cgctcaaggt
    34261 ttttttggat ggctgatgtt tgtctgaaaa tcttgattga gaaataaaaa gccaccctat
    34321 taggtggcta taattgattt agactttcta aaattttcta ttttttactg aatcgcttcg
    34381 accaagtctt gagcttgttt ttcaagtgag tttaggactg tttcttcaag aaccaatttt
    34441 ccgtctgccc aggcagagtc attaacacgt gcagcagtga aatcaccaac gccttgtgta
    34501 cggataaatg gcaagaggtc tttgtagata gcgaaaagtt gatcgtgccc tgcattggct
    34561 acagatgata ctgtgacaaa cttgtcttga agggcagaag cgccacgtgt atcagacaag
    34621 tcaagggcac gagatagcca gtcaagcaag ttttttactg taccagggat agagaagttg
    34681 tagactggag agaaaatcca gatagcatcc gcaacgagaa ctgcttcacg agcagcagct
    34741 acagctggat gtgttggaac ttccaaatct tggctgaaga gaggaagggc tgaataatca
    34801 aggtagctaa cttccgcttt accagcaagt gctttctcag cttcgagcgc catttggtgg
    34861 ttgaaagaac cttgacgtag tgatccgacg ataaataata cttttttaga catgatgtgt
    34921 ctcctttttc ttcaaatttc gagaactctc tcgttgttat aaaatatatt acaacaaaac
    34981 aaaagaactt gcaagaaatt tgctcagagt ttatttgaaa attgtaaaat ttcggaaact
    35041 ctagtcattt gtgactgaaa aagtaagaac tgtttcactt tcaaaatcgg ttttgtttga
    35101 agggagaaat agaat
//
LOCUS       DAVAAL010000010        31360 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVAAL010000010 DAVAAL010000000
VERSION     DAVAAL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31360
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.010"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..549)
                     /locus_tag="VQM60_000346"
     CDS             complement(<1..549)
                     /locus_tag="VQM60_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET1080245.1"
                     /translation="MVKINKICSIQGSSAENEDTVGSQNQYFWIIDGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYYRLPTFAF
                     IFAKLSEKKLEYMMLGDCVMLVNEMEITDHRVDNLFEKGKNEIKDSNGTNSVLNKKII
                     LQKIRKLSNQPSGYWIGSLDERF"
     gene            complement(533..2611)
                     /locus_tag="VQM60_000347"
     CDS             complement(533..2611)
                     /locus_tag="VQM60_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET1080246.1"
                     /translation="MKSFQQNEVFSIRKSKVGVCSVLLAVLFIGTQSVLANEVVGETS
                     SSKDNMQMNSLLSSTSTSLSAEPHSDTLSLDNNSSLSTDNNGGVSINQNKPDITNDTM
                     MGNISGMEVNTILSTNLNEHVALNVKDYGAIGDGVNDDRQAIQDAIDAAAQGLGGGNV
                     YFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIVFMTGLFTDDGAQVEWG
                     PTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNSNNVTIKNVTFKDSYQG
                     HAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEPLTRKGFPYALNDDGKK
                     SENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKILNNHFDNMMYAGVRFTG
                     FTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLLDLNKQVVIAENIFNIA
                     DPKTKAIRVAKDSAEYLGKVSDITVTKNVINNNSKETEQPNIELLRVSDNLVVSENSI
                     FGGKEGIVIEDSKGKITVLNNQFYNLSGKYISFIKSNANEKEPVIRDSDGNFNIVTEN
                     GLYKIVTNNLSDKNEKEKNKEEKQSNSNNVIDSNQKNGEFNSSKDNRQMNDKIDNKQD
                     NKTEEVNYKIVGDGRETENHINKSKEIVDVKQKLPKTGSNKIMELFLTVTGIGLLLTL
                     KGLKYYGKDK"
     gene            complement(3532..4758)
                     /locus_tag="VQM60_000348"
     CDS             complement(3532..4758)
                     /locus_tag="VQM60_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET1080247.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     QYTQTPKTVCLNSATAALELILRVLEVGPGDEVVVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDLFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPAGSIADFTSFSFHAVKNFTTAEGGSATWKANPAID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYS
                     GLLQRRKDIVDRYDRGFAGSRIHPLAHKTETVESSRHLYITHVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMANYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTSSKK"
     gene            complement(4801..5493)
                     /locus_tag="VQM60_000349"
     CDS             complement(4801..5493)
                     /locus_tag="VQM60_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET1080248.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVV
                     LVLTSPIFLIMSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     PAGITSPASIHYKDEDTIISQMTEKGLSVDQAYVEYVLPEKMRYNLAYLREFSFLGDI
                     KIMFQTVFEVLK"
     gene            complement(5677..7419)
                     /locus_tag="VQM60_000350"
     CDS             complement(5677..7419)
                     /locus_tag="VQM60_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080249.1"
                     /translation="MQNKKEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTMVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTTLVLIFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     VIKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDSVDIRAYSQEELRTNIGLVLQDPFLYHGTIKSNIAMYQEISDEQVRAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMS"
     gene            complement(7409..9154)
                     /locus_tag="VQM60_000351"
     CDS             complement(7409..9154)
                     /locus_tag="VQM60_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080250.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLVFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYTIDSFAFENEETLTNVHFSLEKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGTI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLAQV
                     YQDIVAMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGTHDDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9360..9656)
                     /locus_tag="VQM60_000352"
     CDS             complement(9360..9656)
                     /locus_tag="VQM60_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET1080251.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(9809..10264)
                     /locus_tag="VQM60_000353"
                     /pseudo
     CDS             complement(9809..10264)
                     /locus_tag="VQM60_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10398..11225
                     /gene="xth"
                     /locus_tag="VQM60_000354"
     CDS             10398..11225
                     /gene="xth"
                     /locus_tag="VQM60_000354"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET1080252.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTVTFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11237..11488
                     /locus_tag="VQM60_000355"
     CDS             11237..11488
                     /locus_tag="VQM60_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET1080253.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLSL
                     EECQLMLEEGTKDQLAELTYPFGRGVNLSFGIKDVSKLY"
     gene            11495..11623
                     /locus_tag="VQM60_000356"
     CDS             11495..11623
                     /locus_tag="VQM60_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET1080254.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPNGYFLRFSE"
     regulatory      12006..12101
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12501..13082
                     /locus_tag="VQM60_000357"
     CDS             12501..13082
                     /locus_tag="VQM60_000357"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET1080255.1"
                     /translation="MKLLEDRILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13082..14344
                     /locus_tag="VQM60_000358"
     CDS             13082..14344
                     /locus_tag="VQM60_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET1080256.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPIP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14395..14856)
                     /locus_tag="VQM60_000359"
     CDS             complement(14395..14856)
                     /locus_tag="VQM60_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HET1080257.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(14853..15617)
                     /locus_tag="VQM60_000360"
     CDS             complement(14853..15617)
                     /locus_tag="VQM60_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HET1080258.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(15798..16202)
                     /locus_tag="VQM60_000361"
     CDS             complement(15798..16202)
                     /locus_tag="VQM60_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET1080259.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(16272..17753)
                     /locus_tag="VQM60_000362"
     CDS             complement(16272..17753)
                     /locus_tag="VQM60_000362"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET1080260.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNYPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(17772..18950)
                     /locus_tag="VQM60_000363"
     CDS             complement(17772..18950)
                     /locus_tag="VQM60_000363"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET1080261.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            19115..20122
                     /gene="galR"
                     /locus_tag="VQM60_000364"
     CDS             19115..20122
                     /gene="galR"
                     /locus_tag="VQM60_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET1080262.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(20168..21205)
                     /locus_tag="VQM60_000365"
     CDS             complement(20168..21205)
                     /locus_tag="VQM60_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET1080263.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(21206..21559)
                     /locus_tag="VQM60_000366"
     CDS             complement(21206..21559)
                     /locus_tag="VQM60_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET1080264.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(21803..22693)
                     /locus_tag="VQM60_000367"
     CDS             complement(21803..22693)
                     /locus_tag="VQM60_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET1080265.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            22826..23362
                     /locus_tag="VQM60_000368"
     CDS             22826..23362
                     /locus_tag="VQM60_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET1080266.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(23529..24335)
                     /locus_tag="VQM60_000369"
     CDS             complement(23529..24335)
                     /locus_tag="VQM60_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET1080267.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(24680..26200)
                     /locus_tag="VQM60_000370"
     CDS             complement(24680..26200)
                     /locus_tag="VQM60_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET1080268.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(26193..26921)
                     /locus_tag="VQM60_000371"
     CDS             complement(26193..26921)
                     /locus_tag="VQM60_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080269.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(26936..27700)
                     /locus_tag="VQM60_000372"
     CDS             complement(26936..27700)
                     /locus_tag="VQM60_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET1080270.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(27704..28147)
                     /locus_tag="VQM60_000373"
     CDS             complement(27704..28147)
                     /locus_tag="VQM60_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET1080271.1"
                     /translation="MKYCHLVAHHIRLLNGRIFQKLLRQDPDSLYRSEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKNLEEQNLVTVSPCGEDKRKKYLVLTELGKSQ
                     KEVGQRVSQRLDTICYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(28829..29125)
                     /locus_tag="VQM60_000374"
     CDS             complement(28829..29125)
                     /locus_tag="VQM60_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET1080272.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(29147..30211)
                     /gene="pepA"
                     /locus_tag="VQM60_000375"
     CDS             complement(29147..30211)
                     /gene="pepA"
                     /locus_tag="VQM60_000375"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET1080273.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            30612..31331
                     /gene="galE"
                     /locus_tag="VQM60_000376"
     CDS             30612..31331
                     /gene="galE"
                     /locus_tag="VQM60_000376"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET1080274.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGRNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTH
                     DGSCIRDYIYVLDLHRLMLKE"
BASE COUNT         9289 a         7048 c         5645 g         9378 t
ORIGIN      
        1 aaatctctca tccaacgaac ctatccagta cccactcggt tgattagata attttctgat
       61 tttttgtaaa ataatttttt tatttaaaac actatttgta ccatttgaat cttttatctc
      121 atttttccct ttttcaaata agttatctac tctatgatca gttatttcca tttcgtttac
      181 taacatgacg cagtcaccta gcatcatata ctccaacttt ttttctgaaa gtttagcaaa
      241 tataaaagca aatgttggta agcgataata ctctgttaat tcatatgagt ttaaaccgat
      301 ttcatcttta acctcgagta aagcagtttc aaatatttgt ttaagagttt ttgattcttt
      361 acaattaacc gacaaacttt ctgataaaat atgtacaact tctgagactg aataacctat
      421 ctcctcttta gaattatata aatctgtagc tccatcaata atccaaaaat actgattttg
      481 tgaacctaca gtatcctcat tttctgcgga acttccttgt atcgaacaaa ttttatttat
      541 ctttaccata atacttcaac ccttttagtg tcaaaagtaa accaattcct gtcactgtta
      601 agaatagttc cataatcttg ttcgaaccag tctttggtaa tttttgtttt acatctacta
      661 tttctttaga tttattaata tgattttcag tttctctgcc atctccaact attttatagt
      721 ttacttcttc tgtcttatta tcttgtttat tgtcgatctt gtcattcatt tgtctattat
      781 ctttacttga gttaaactct ccgttcttct ggttactatc aattacatta tttgaattag
      841 attgtttttc ctctttgttt ttttcttttt cgtttttatc acttaagtta tttgttacaa
      901 ttttgtaaag cccattctcc gttacaatat tgaaattacc atcgctatca cgtataacag
      961 gctctttctc atttgcatta gatttgatga atgatatata cttaccggat aaattataaa
     1021 attggttatt taaaacggtt attttaccct ttgaatcctc aataacaatt ccttctttac
     1081 caccgaatat actattctct gagactacta aattatcact aactcgtaat aattcaatat
     1141 ttggttgttc tgtttcctta gaattattat taattacatt ttttgttaca gtaatatctg
     1201 atacttttcc taaatattct gcactatctt ttgcaactcg tatcgctttt gttttaggat
     1261 cggcaatatt aaatatattt tcggcgataa ccacctgttt atttaaatct aataggtctt
     1321 tagtgttttt atagctataa gcatttacta aagctgctcc gctttctcga taatgtacac
     1381 tctctccttt aactttctta tcaaagcgat ttcctttgat taatacatca gtgaatcctg
     1441 taaaacgtac acctgcatac atcatgttat caaaatgatt atttaaaatt ttaatattag
     1501 aggggttctg tgtcgacaat gtttgatagt gtgtgccaat tgctgttact aattccccag
     1561 atttatcact tttgccaaaa taggaatttt gaatagtcac attttcagat tttttcccat
     1621 catcattcaa ggcataagga aaaccttttc tagttaatgg ttcaatctga atgctctcct
     1681 tacttatgat ttgcccatcc ttcatcgttt tgggtaaggc ttgcccaaga aaacgagaat
     1741 tatcaactaa tacatttttc gaacctgcaa tttgaatagc atgcccttga taactatcct
     1801 tgaatgttac attttttata gttacgttat ttgaattccc aatagcaaat gcacctgaag
     1861 aatttataag tggtagattt tttgctttag ttccttcttc attcaaagca ccgttcatat
     1921 caatagtacc accagaataa ctaatatctt ctgttgggcc ccattctact tgcgcaccat
     1981 catccgtaaa taaacctgtc ataaaaacaa tagaagggtg attcttaata tttataccat
     2041 ttagaatggt agctttctca ttcaattcta agtgtgtatg actttttaaa aaaacaattt
     2101 cttttactaa ataagttcct tccggaaaat atacatttcc tccacctagc ccttgagctg
     2161 cagcatctat tgcatcttga attgcttgtc gatcatcatt aaccccgtca cctattgctc
     2221 cataatcttt aacattcaaa gctacgtgtt cgttcagatt agtacttaaa atagtattca
     2281 cttccatccc agagatgtta cccatcatag tatcatttgt tatatcaggt ttattttgat
     2341 taatactcac tcctccatta ttatcagtac ttaaacttga attgttatca agactgagag
     2401 tatcagaatg gggttccgca gataatgatg tagatgtgct actcaacaaa gagttcattt
     2461 gcatattatc tttagacgaa gaagtttcac caactacttc attagctaaa acactttgtg
     2521 ttccaataaa taaaaccgct aagagaactg agcaaactcc caccttagac ttacgaatag
     2581 agaaaacctc attttgttga aaagatttca tattagcctc caattaaaaa tattatgtcc
     2641 taatttaata ttcatataaa ttccatacca gaaaatgaca ccgctgtcat tttctttata
     2701 atatattttt aaatcaaaat tgtcaaggaa taacacaata aaatatcaaa attgtgaaag
     2761 cgtgaccatt tatatatcta accatacttc taataattct ttaaccttcc attcaaaagt
     2821 gattactatc tttcttagct tgaatataaa aatatcctat tcactgtatt taaagcatgt
     2881 tgacatctcg taaaatacta atttataaat acaagaacaa atatcttgtc atgcaagcat
     2941 tcgccaggta tatttacatg aatatcgttc atatagcgga ttgaaatcaa aacaatacaa
     3001 tatatttctt aaatagattt tctataaaag ctagattcaa gctctataaa aatagacgca
     3061 gttcttctaa aatactggaa taggacatat aaatcattgt agacacgtgt tttatagatt
     3121 atttcaaaag aatgactaga acatgaaaaa tagtctcaaa actttgaaca atattctgaa
     3181 aacaaaaaag aaagacgcga gtttccacaa cctcatcgtg gaaactcgcg tctttcaaaa
     3241 aatagggtga attgaaaact agaacaagac actacatttt ctaaatgatg cctaataaaa
     3301 attctaaatg ctacaccaaa ttgctcacat ctcatttcaa accatacagt atattgaaac
     3361 tataagagta cacctttgct tctaaaatat tgctagaaat taatttgact atcctgattg
     3421 atttgtcctg tttttatttc attttactat aggactttca attgaaaaca acagttttac
     3481 actcaattag actttttaga aacagaatca ctattacttg actgtagttt gtcatttttt
     3541 tgatgaagtt agcacttttt cagaaactgt tttgaaagtc tcaatgatat agtccacttc
     3601 ttcatcactt aatttagtgt gaagaggaag ggtaatttca ttttcaaaga aggcataggc
     3661 cttaggatag ttcgccatgt caaaaccaag attcttatag gctgtcaaga gcggaagcgg
     3721 tttgtagtgt acgttacttg caattcctgc cttggccaat tcttggatga taagattgcg
     3781 ttcttctaag cttgctcctt ctacatgggt aatgtagagg tgacgtgaag attcgacagt
     3841 ttcagtcttg tgtgctaaag gatggatgcg agaacctgca aaaccacgat catagcggtc
     3901 cacaatgtcc ttacgacgtt gcaacaaacc agagtaacgg tccaattgca ccaaaccaag
     3961 cgaagccatg atatcggtca tattgcactt gtaggctggt gtgacgatat cgtattccca
     4021 tgaacccagt tgcatcttag caagagcatc cttagtttgc ccgtgaaggg aaagaatttg
     4081 gaattctttg tacatctctt cgtcgtcaat cgctggattg gctttccaag tcgcacttcc
     4141 accttctgct gttgtaaagt tcttaacggc atggaatgag aaggaagtaa agtcagcgat
     4201 agaaccagct ggttgtcctt tataagtaga tcccaaagca tgggcactat cagaaacaat
     4261 cacgatacgg ttaaaggctt tttgccactt gcttgaagca gtaaagaggt cacgtttctt
     4321 ctccactact tggaacaaac ggtcatagtc gcaaacaatc cctgcaagtt ctactggaat
     4381 gattaccttg gtcttttcag tgatagcttg ctccagcaag tcataatcca tctcaaacgt
     4441 atctgcttgg atatccacca tgacaggtgt tgctcctacg tgagtgatga cactacatga
     4501 agctgtatag gtcatagctg gaacgacgac ttcatcacca ggccccactt ccaagacgcg
     4561 caaaatcaac tcaagagcgg cagtcgcaga gtttaggcag acagtcttgg gtgtctgtgt
     4621 gtattgagac aagcgacgct ccaattcttt tgtcttagga ccggttgtga tccaaccaga
     4681 acgaagggta tccgctactt cagcaatttc tgcttctgta atatcgggtg gtgaaaatgg
     4741 aatattgtaa tttggcattg atttgctcct tttatctgta ctcattttct catgactact
     4801 ttattttagc acttcaaaca cggtttgaaa catgattttg atgtctccaa ggaaactaaa
     4861 ctctcggaga taggctaggt tatagcgcat cttttcaggt agaacgtatt cgacataggc
     4921 ttggtcaacc gacagacctt tctccgtcat ttgactgatg atagtgtcct catccttgta
     4981 gtgaatgctg gctggagagg taatccctgc tggcaagagc aaggtcgcca tcatttcagg
     5041 gctatactgc tctgtgtaac gaggcacttc aggacgcgtc cctacaaaag acatctcgcc
     5101 tttaaggaca ttgaccaact gaggcagttc gtccaaacgc acacggcgga tgaaattacc
     5161 aaccttggta atgcggctat cgttagcaga agtcactaga cttccttttt tatccgcatc
     5221 cgtcaccatg gtacggaact tccaaatctt gaaccgacgg ttgtactggg tcacgcgctc
     5281 ttgcttgtaa atcactggcc ccttgctatc caacttgatc caaatgctca tgatgagaaa
     5341 gatgggagag gtcagaacca gtacaaccaa ggccaaaacc cagtccaaac aacgcttgaa
     5401 aatcagcgaa cccttccttt tagagacaag ctggtagtaa gactcaacct cgcttgattt
     5461 catttccacg ggcaagtctt cccatttcag catgctgttt ctcctttgtt tttattatac
     5521 tatttgtcaa catttttcat tataccacaa aatggaaaag gcggtgaaag aaatctgtaa
     5581 tttgaaggat ttccttctta cactcaatga aaatcaaagt acagctttta ggttgcagat
     5641 aaagctgaca tggcttgaat agattttcaa agagtattaa gacatggctc ctgcctgcaa
     5701 gctatacatc ttgtgatagg ttcctcccaa ggccaaaagt tcctcatggg ttccgctctc
     5761 gataatgcgc cccttgtcca aaacatagat acagttggcg tcttggatgg tagagagacg
     5821 atgggcgata gcaatggttg tccgtccctg tctcatctta gccagagaag cctgaaccag
     5881 actttctgtt tcagagtcaa tattggctgt cgcttcatcc aaaatcagga ttttaggctg
     5941 gctggcgact gttctggcaa aggcaagaag ttgacgctga cctgttgaaa aactggagcc
     6001 acgctcggaa acaggggaat cataaccctg aggaagttct tgaataaagg aatctgcatc
     6061 cacgaaggct gctgcagccc ggacctgctc atcactgatt tcttggtaca tggcgatatt
     6121 ggacttaata gttccgtgat agaggaaggg atcctgcaag accagaccga tatttgttct
     6181 cagctcttcc tgactgtagg ccctaatatc cacactatcc aagagaactc gccctgactg
     6241 aaattcataa aagcgcatga ggacattgat aatcgaagat ttccctgaac ctgtatgacc
     6301 cacaaaggca atggtttcac ccttattaac ggagaaggaa atgtcatcca gaatcggatg
     6361 ttttccatca taagagaaac atacatgttc aaaacggata ttgccttctt tgatcacggc
     6421 ctgtccattt tcttgaagag gctcataggt tctctcgtca atcagagcaa agacacggcc
     6481 tgcagaaacc atggaagttt gaagggttga aaagttttgc gtcacctcaa tcaaagggtc
     6541 aaagaggcgg ttaatgtact gaataaaggc atacatagtc ccggcagtta tcccgataga
     6601 aaaaccacgg tagccaaagt aggccatcaa gactgcatag cctagaagtt tcagcaaact
     6661 catagcaggg cgcaaaaaga gggcatccaa ggctacagaa cggttggcat agaccaagtg
     6721 ctcttggttg atttcatcaa attctgcctg caggcgcttc tcctgattaa aggcctggat
     6781 tattctgatt ccctcgatgt tttcagccag cttactattg atatctgata agagacttct
     6841 ggttttttcg ataatcttga cggatttttt ccgatagaga ttgaccaaaa ggaaaatcaa
     6901 gggaagaaag atcaagacta aagtcgtcaa acgaaaatcc aacaccaaca tggtataaag
     6961 ggttgtcaga aagatgaaaa ctgctgagat aaagctggac aaaatccccg aaaacatatc
     7021 actgatggtc tcggtatcat ttgtcaaacg agagacgata gagcctgctg gcgtcttgtc
     7081 aaaataggac atgcccaatt tctccatatt agcaaaggca tcccgacgaa tatccctaac
     7141 aatactgtag gacacccgtg caaagagaag attaccgaca tactgtacca tagtttgtag
     7201 gatataaagg ccatagtaga ccagcaaaac ggtcacagcg agttggttga gattactcag
     7261 atactggtca ataaagtggg aagccacaag gggaatgaca cttttgatga ccgtcgtcgc
     7321 taggagaaaa ctgagtgcca aaaaagtcag gagtccataa ggcttgagat aagacatcaa
     7381 gcgcttcaat acagcccatt gttctttctt attctgcatc ttcttctccc ttcatttcca
     7441 gttgctgaga ctggtaggtt tgggcatacc agccatccaa agctagcaag tcatcgtgtg
     7501 tacctcgttc gatgatttga ccattttgca ggactaaaat caaatctgca tgtacaactg
     7561 cactgaggcg atgagccgtg ataatggttg tcttgtcctt acgtgtttcc ttaagattgt
     7621 cgataatcgc atactctgtc ttggcatcca cggccgacaa agaatcatcc aaaatcaaga
     7681 tatcagggtc taaaatcata gcccgactca tggccagacg ctgcttttga cctccagaaa
     7741 gactgactcc tttttctcca atcagcgtat caaatccctg aggcatggct acaatatctt
     7801 ggtaaacttg ggctagctta gttgcttcct cgactgctga aaggggcaag ttaggattgc
     7861 caaagcgaat attgtctaaa atcgaagtcg caaagaggaa ctgatcctga ggaacatagc
     7921 ccataagact acgaagatct gtcagacgat agtcacgaat atcgtgacca tttagataaa
     7981 tggttccctt gtccacatcg tattcacgca agaggagctt gatcaaagat gttttcccag
     8041 agcctgtttg cccaaccaag cccagggttt gacctttttc caaactaaag tgaacatttg
     8101 tcagtgtttc ctcattttca aaggcaaagc tgtcaatggt atactccaaa cgcccatttt
     8161 caataccatc cagaggaaac tcagggtctt gtacaggaga ttcctgagac aaaagatttt
     8221 caatccgctg gtaagaaacc ttccctcgct gagtagtatt aaagaggaag ccgatggcca
     8281 taagaggcca gaccagcata tccaaatagc tgataaaggt gactagattc ccaactgtaa
     8341 tctgcccttc ctgaaccatc aaggagccaa ccaaaagcgt taaaacatag gacgaaccaa
     8401 caaacacgag aaccataggg tcaaagagac tatcatattt catggtttgc aggttctttt
     8461 ggaaggttaa ttcattgact gcctgaaaag acttcaactc gtctgcctga taaccgaaag
     8521 acttggtcac tttgatacct gatacggact cctgtacctt gttattgagt tcagaaaaag
     8581 cagcttggga ttcgccaaag gccttatgag tctttctccc taggcgacta gttgcatagg
     8641 ccatgaaagg tagggggaga atggcaacaa gagtcatctg ccatgagatg ctaaagagca
     8701 tggtcaacaa agtcaccaga gccgtgatag aggcatccac cgcagacatg acaccgccac
     8761 ctgctaaacg agtcaaggca ttgatatcat tggttgcgtg tgccatcaga tcacccgtcc
     8821 gataggtttg ataaaaggct gatgacattt ttgtgaaatg cttaaacaag cgagaccgca
     8881 tgatctgtcc caagcaataa gaggtcccaa ggatatacat acgccacaca tagcgcaaat
     8941 agtacatacc aaaggctgca agtagcaagt aaaataggct aagaaggagg tcctgctggg
     9001 ttaattgccc cgatgtgatg gcatcaatga cccgccccat aaccatagga ggaatgagat
     9061 tgaggacgga aaccaagacc aaggccacaa tcccgactag ataacggcgt ttttctaact
     9121 tgaaaaacca ccagagtttt tgaataatgg acataaaatc cctttctgat cgcaaatgga
     9181 aacctgaggc caatactcaa tgaaaatcaa agagcaaact aggaaactag ccgcaggctg
     9241 ctcaaagcac tgctttgagg ttgtagatag aactgacgaa gtcagtaacc tacatacggc
     9301 aaggcgacgt tgacgcggtt tgaagagatt ttcgaagagt acaagacccc aggtttttct
     9361 tattcataag ttacgactgt aacagcaccc ttgtcatatt cagcaataaa gatattggct
     9421 acattgtcat gcccttgttt gctgaggtta tcaagcaacc actcttcgct acgaccaatc
     9481 gattccaaga catctacttg aatcacaccg tcagtcacaa ctggatactt aggattttca
     9541 tctcccattt gcacaacgat gagttgccca ttttgctctt gcacagctcg tttgacttgc
     9601 ttcatctgga aaatcccttg gctacgaagt ttgagggcta catccgatgc agacaaacca
     9661 actgaacgac aggcttatgg gtcaatattc ccatttttga taaggagagt tggcttacca
     9721 tcaatcaaac gtttcacaaa gtgaacattg ttgttgagct atttgagagt caagaccagt
     9781 ttgattaaaa tttcgatata attaagtgtc atttgttcac acccaccagt tcaattgcgt
     9841 ctgtcttata caattcgact ttctctaaca ggtacttgtc tggctcactt ccgtttaggg
     9901 cacgataata gcgatctccc accttgataa gtgcgccatc tgtgtttgtg gaagtattaa
     9961 catagacttc tgacttgtct actcccaaat ctttggaaac aacctcgatg aaatgaagtg
    10021 aagtttgaaa tttattgtta gaaacttgat tgttttgata agtcgtgatg ctgaccaaaa
    10081 gcagagctaa ataaggccag agctgaaatc atgaccaact cacgaaattc ggttcccttt
    10141 ttatcatggt atgccttaaa agcaaaaaaa ctgtcacagc taatagaaca atcccaaagc
    10201 caaccatgat accattttgc tgaccgattt ggctgagtac atagtcataa gagtagaatt
    10261 tcatgtattt tcactccctt tcataaaatc attcttaatt ataaacgaaa gagagtaatt
    10321 tttcaaacta tacgttgggg aaatagatct ttttttggta taataaaatc tataatctga
    10381 atgaaaaagg taactttatg aaacttatct catggaatat tgattcccta aacgctgccc
    10441 taactagtga ctcagctcgt gccaaattgt cccaagaagt cctacaaacc ttggtcgctg
    10501 aaaatgctga tatcattgct atccaagaaa ccaagctttc tgccaaagga cctacaaaga
    10561 aacacgtgga aattttagaa gaactcttcc caggctacga aaacacgtgg cgctcttccc
    10621 aagagcctgc ccgtaaaggc tatgctggaa ccatgttcct ttataagaaa gaacttacac
    10681 ccactgtcac tttcccagaa atcggtgccc cttctaccat ggacttggaa ggccgcatca
    10741 tcactctaga atttgatgca tttttcgtga cccaagttta cactccaaat gctggcgatg
    10801 gtcttaaacg cttggaagaa cgccaagtct gggatgtcaa atatgcggag tatttggctg
    10861 aactagacaa agaaaaacca gtccttgcga ccggtgacta caacgtagcc cacaatgaaa
    10921 tcgaccttgc aaatcctgct agcaaccgcc gttcacctgg atttactgac gaggaacgtg
    10981 ctggatttac caaccttttg gcaactggat ttacagacac cttccgccat gttcatggcg
    11041 atgttcctga acgctacact tggtgggcac aacgcagcaa aacttctaaa atcaacaata
    11101 caggctggag aatcgactac tggctcacaa gtaaccgtat cgctgacaag gtcactaaat
    11161 ctgatatgat tgactcaggt gctcgccaag accatacacc gattgtcttg gaaattgact
    11221 tgtaagaatt ttctatatgg actacaatgc ggtcattccc gagttgcttg tatcgaatat
    11281 cgaacagtcc cggtccttct actgtggttt actgggtttt aggattgaat accaacgtcc
    11341 agaagaaaac tttctctttc tttctcttga agagtgtcaa ctaatgttag aggagggaac
    11401 aaaggatcaa ctagctgaac tgacctatcc atttggtcgt ggagtcaatc tctcctttgg
    11461 cataaaggat gtttcaaaac tctattaaag agtgatgaag gcaaactatc ctatttatcg
    11521 ccccttgacc aaaagaaagt ttcgtgtcag tgatccctat atctatcctc ataaatttgc
    11581 agttttggat ccaaatggct attttttaag atttagcgag tagaataata aaaattggga
    11641 aaagtcttta aagttagaaa aaggcatatt gtcaggtgtt taaaaacttg atagtatgcc
    11701 ttttatgatg gaaaaatctt ctaatactct tcgaaaatct cttcaagcca cgtcaacgtc
    11761 gccttaccgt aggtatatga tactgactct gtcaattcta tccacaacct caaaacagtg
    11821 ttttgaggtt gtggatagct tcctagtttg ctctttgatt ttcatttagt ataaatatta
    11881 gcttacaatg gagtttttgt tcaccctctc ttctgtttgt tcgtttttta aaccaaaaca
    11941 ctaacaaaat agttgattta taaagttatt tagtataaaa taaaaacatt ggctcaagcc
    12001 tatttaaaat tgaatatcgt tttacttgtt tatgtcgtga attggcacga cgtttctaca
    12061 aggtgccgga acacctaaca ataagtaagt cagcagtgag atatggccgt ttttgccatg
    12121 cctatttggg ggacttactt agagattaag taggtctttt ttgaatattt ttaatatttc
    12181 ccttaattag tgcggacggg aggtgaaatt atcctgtgaa taatttcagg aatccaggga
    12241 atttcatccc taatttttga gcccctagct ctttcatttc taggcttagg ctaaaatagt
    12301 tccccgaact attttactct caaaaatccc gtcatagaca ataacaagat cattgtctat
    12361 gataccacta caacgggaaa tatttgataa gactattacg agcgaagcga gtcttatcaa
    12421 atatatagta ttagcaaaag ggatcactat agaaacattt caacaacaag taaattagct
    12481 tttagacttt aggaggtctt atgaaattat tagaagatcg catcctcaag gatgggcata
    12541 tcttgggtga taacatcctc aaggtagatt cctttttaac ccaccaagtt gactttagct
    12601 tgatgcgaga gattggtaag gtttttgcgg aaaaatttgc tgctgccggc attaccaagg
    12661 tcgtaaccat tgaagcgtcg ggtattgccc cagccgtttt tacagctgaa gccttaaacg
    12721 ttcccatgat tttcgccaaa aaagctaaga acatcaccat gaacgaaggc atcttaactg
    12781 ctcaagtcta ctcctttacc aagcaggtga ccagcaccgt ttctatcgct ggaaaattcc
    12841 tctcaccaga ggacaaggtt ttgattatcg acgatttcct tgctaatggc caagctgcta
    12901 aaggcttgat tcaaatcatc gaacaggccg gtgccacagt ccaagctatc ggtatcgtga
    12961 ttgagaaatc cttccaagat ggtcgtgatt tgcttgaaaa agcaggctac cctgtcctat
    13021 cacttgctcg cttggatcgt tttgaaaatg gtcaggtcgt atttaaggag gcagatctct
    13081 aatgcaaact caagaaaaac actcgcaagc agccgttctt ggcttgcagc acttactagc
    13141 catgtactca ggatctatcc tggttcccat catgattgcg acagcccttg gctattcagc
    13201 tgagcagttg acctacctga tttctacaga tatcttcatg tgtggggtgg caaccttcct
    13261 ccaactccaa ctcaacaaat actttgggat tggactccca gtcgttcttg gagttgcatt
    13321 ccagtcggtc gctcccttga ttatgattgg gcaaagccat ggtagtggcg ctatgtttgg
    13381 tgcccttatc gcatctggga tttacgtggt tcttgtttca ggcatcttct caaaagtagc
    13441 caatctcttc ccatctatcg taacaggatc tgttattacc acgattggtt taaccttgat
    13501 ccctgtcgct attggaaata tgggaaataa cgttccagag ccaactggtc aaagtctctt
    13561 gcttgcagct attactgttc tgattatcct cttgatcaac atctttacca aaggatttat
    13621 caagtctatc tctattttga ttggtctggt tgttggaact gccattgctg ctactatggg
    13681 cttggtggac ttctctcctg ttgcggtagc tccacttgtc catgtcccaa ttccactcta
    13741 ctttgggatg ccaacctttg aaatctcatc tattgtcatg atgtgtatca tcgcaacggt
    13801 gtctatggtt gagtcaactg gtgtttatct agccttgtct gatatcacaa aagatccaat
    13861 cgacagcacg cgccttcgca acggttaccg cgcagaaggt ttggccgtac ttctcggagg
    13921 aatctttaac accttccctt acaccggatt ttcacaaaac gttggtttgg ttaaattgtc
    13981 aggcatcaaa aaacgcctgc caatctacta cgcagctggt ttcctggttc tccttggact
    14041 gcttcctaag tttggtgccc ttgcccaaat cattccaagc tccgtcctcg gcggtgccat
    14101 gctggtgatg tttggttttg tatctattca agggatgcaa atcctagccc gagttgactt
    14161 tgctaacaat gaacacaact tccttatcgc agctgtttca atcgctgcag gtgtcggtct
    14221 caacaacagt aatctctttg tcagcatgcc gacagccttc caaatgttct tctcaaacgg
    14281 aatcgtcgta gccagcctac tcgccattgt cctcaatgcc gtattaaatc ataaaaagaa
    14341 ataagaaaaa gaggtgcgag cctctttttt gtgtgacaaa ataaaaacat ctacctattc
    14401 atatatgcta gtgtcatagt cctgaaaatc atctgcatca agaacaattt cacaactctt
    14461 atacttttct cttacaagtc gttcggcttc atctggattt tcagcctcta tacttactaa
    14521 acgtgataaa gtttctgtaa tttctactgt atactttttc agttttagat tgctttccaa
    14581 taattcacct attaacaata atctatgata ggagctatgt agccactctg gcaatttatc
    14641 tcctacttcc cacccatatt tttccttcat caaagctagt tgtttttcgt caccaaattt
    14701 catattttca gataaccatc tcacacaatc gggatctgat aaggtacaga cgattaaact
    14761 taggttatcg taacggcttt cagtttcata atcaggccag atgactttta agaaatcata
    14821 cacctcttct gatgcaacaa gttgtttcgt tttcataatt tccgatactt tcctctacct
    14881 ttaaattcta ttatttcttt gtctcttaat atttgaagct gttgcctaat cttttctttg
    14941 atatgattgt tcttaacaaa gatatttttt aggtcacttt caaaacgata catatcttca
    15001 agggtaaatt ctgaaccctc tatcttatct atacaattta gaatttctat tgtccaacct
    15061 cttgatgaca gagagctctt ccttaaaaat aaaccttgct taaattcttt tgtaactttt
    15121 tctggatctc taacttgtcc atcttgcaca agaaatatcc ttcctttaga aggtacttgt
    15181 gataaatcaa tgttacaacc aatccaacct gctcgtctag cagttggtgc aagtggtttt
    15241 ctttgaataa tcgatttcgg tgtaacaaat tgcttcggaa ggacaagaaa gttatttacc
    15301 tcaaaatttt ttgtgtaagt taaaaaaaag aaattaggat tattatctgc ctgcacacgc
    15361 ttcatcatcg ttgcataagc accatcattg attgttgatg aaaaatttcc ttttttgctc
    15421 tttagttcaa actcctcact acaatgatta cagtaaaaat ctgctacagg ccgattattt
    15481 tcaaaatgat ttaagggatt gttcccacaa ttagggcaat aagactgacg ataaacccaa
    15541 tcctccgtta agatacgtgc cttttggcta ttacttttat aggtttctac taattctaaa
    15601 ttaaagtgta attccattta aaaattcctc actaatatga gaactcactc gatataagca
    15661 attaaaatct actataacta gtataccaca tatatgtacc tattacattt gtatttcatc
    15721 actctctttt atatttcctt ctaacatatc tcataaatta aaaatgagtg agataaattt
    15781 atcccactca cctttcttta tatacttacc cgtctttcct cagacctttg gcctgtgaca
    15841 aacatggtaa aggttgcttt acagacattt ctgccctctt gattggtgat atccacatcc
    15901 atcacacagg ttgtacggcc ttgatggaca cattctcctt taatggtcaa tacatcgtcg
    15961 agttttcctg ccttaaggta gttgatagag gattggagtg tcaccccatc aagtcccagc
    16021 gagataacca ccaaaccact gatttggtca catagcgtga atagatagcc accatgggca
    16081 ttgccatagt agttgagcga cgagttcact actttggtcg tcaccacaac gtgaccatct
    16141 ctcatttttt caatttcgta attttcaaag gcagatatag cgtcaaaatg aaagtctttc
    16201 atcgtttcct cctgatattt caaacgacac tatgatacta ctttttatag gactgtgcaa
    16261 ggagaaagct cctagtctag taaaattccg acatgttcca caaagcgcat aaaggctgtc
    16321 tgcccttgtt ctgtctgctt gtagactcct gcatcctcaa gtacacgcgc aaagatagca
    16381 cccacagagt ccttgacgat tgcaagggct ttttctttat ccgttaggtc tggatgttgg
    16441 gatttgagtt ggtctgccca ctcctgatga taatcggcaa ctgtaacagc ttctcctaca
    16501 agatagctcg cgacttgctc cacttcttct ttcagacgtg gtggcaagat tgccaagccc
    16561 atgacctcaa tcaagccgat attttccttc ttgatatgtt ggacatcctt gtggggatga
    16621 tagatgccat caggatgctc tgctgaagtc tgattgtctc gcaagaccaa gtccaactca
    16681 aactgtccat cacgcttacg cgctattggt gtgatggtgt gatgcggtgt cccgtctgtc
    16741 tctgctaaaa tctgcacact agaatctgaa tactgacgcc attcctggaa aatcttatca
    16801 gctaagttaa tcaaatcctc tttggaatcc gaagtcaagc gtaggacaga cattggccac
    16861 ttgataattc cagccttgac ctgctcaaaa ccagcaaatc ggaaggtctt ttgcaaggga
    16921 gccaattcca taggaaatac gtgacggcct ccctgataat gatcgtgagt tagaatagag
    16981 ccccccacaa tcggcaggtc ggcattagat ccagcaaaat atcctggaaa ctgctctacg
    17041 atagccaaca gacgttcaaa actctgacga ctaatggcca tgggacgatg ctggccatct
    17101 aaaaagatac aatgctcatt aaagtaagca tagggcgaat actggaaccc ccattcctga
    17161 ccaaccattt caaaacggat aatccggtgg ttgctgcgag ctgggtagtt gacccgacca
    17221 tggtaaccct cattctctaa acaaagttga cactgaggat aattactagc ttgcaccaac
    17281 ttggctaccg caatctcttt cggatccttt tcaggcttag agagattgat ggtaatttca
    17341 agttctccat aatcagatgg aacacgataa gcgatatttt tagcaatggc cttgagtttg
    17401 atgtaatcat ttttctgact gagttgataa aaatcctcta tcgcttgctc aggagagtgg
    17461 acataggctt cccagaagtc acgattgacc tgactcggat aaggagtcac caagtccatc
    17521 agttcagtac caaggatttc acgcgcagtc tgactatcct caatcatctc taatcgaacg
    17581 gcttcctcaa ctagctggtc cttgaggtca atcaatttat ccagattggt ctcaacttcc
    17641 aaaacacctt ctcccactcg tgccaaaaca cggttggtca ggtagattcg atccatttcc
    17701 tcaaatgaac tttcagaaat gacatgtgtt acaaatttat ctactaagat cactatagag
    17761 cctccttttg actagtcaag gacgcgagtg ccacctgcaa cttcagcgat atagaagctt
    17821 ggagcgtatc caactacttc ctcgtagtgt ttgcctacag cttccttaaa ggcctcaaca
    17881 gtatcttttt gaaccaaggc aatggcacag ccaccaaaac cagcccctgt catacgagca
    17941 ccgagaactc cttcttgtgc ccaagctgtg tgaacaagag tatccaattc caaaccagtt
    18001 acttcataat catgctccag agaaacgtgt gacgcattca tcaaacgacc aaatgtttcc
    18061 aaatcacctg cttgaagggc tgcttgagct ttaagggtac gttggttttc aagcacagca
    18121 tggcgagcac gtttcaaacg attttcatct ttaatcaaat agctatattg gtcaacggcc
    18181 cactcgtcca attcacccag ggtctgaata tccaaggcaa cttgcaattc ttccactgct
    18241 ttttcacact cagcacgacg ttcattgtat ttagagtccg ccaattcacg gcgtttgttg
    18301 gtgttcatga taacaacgac attgtccttc aaatcaagtg gcaccaagtc gtattctaaa
    18361 gtattggtat ctaagtaaat agcacgttgg tcagccccca taccaatagc aaactggtcc
    18421 atgataccag agttgactcc gataaagttg ttttctgttt gttttccgat tttaaccaaa
    18481 tccaaacggt ctagttttaa atcaaagaga tgctctgcca cgactcctgt caagagctcc
    18541 aaggatgctg aagaagacaa gccagcacca tttgggatat tcccataaac ataaaaatca
    18601 aaacctttgt caatcacgtg cccagcttct tgcaagaaat gaagaacccc ttttggataa
    18661 ttggtccagt tgtgctcttt ttcaaacttg aggtcagcga gaggcacttc gataatgccc
    18721 ttgtcctcaa agttagctga gtagaaacgc aagacttggt cgtcacgctt acgagctgca
    18781 ccgtaagttc ccaaggaaat agcagcagga aaaacgtgcc caccgttgta gtctgtgtgt
    18841 tcaccaatca aattaatacg gcctggtgaa aagaaggttt ggtctgcttc ttgaccaaaa
    18901 acagcaagaa agtctttgcg aaggacttca gtagtaagat gttgtgccat atgaattctc
    18961 ctttgactgt ccgttaagcc atcttaacgt gtaatcgttt tctttctatt gtatccaagg
    19021 aatttcccaa ggtcaatcct ttttactaaa attttactaa aatatcagta aaaagaagaa
    19081 aaatatgata tactaaccta atcaaggagg atttatggct accttaaaag acattgcaca
    19141 gctagcctct gtctctatcg cgaccgtatc ccgtgtcctc aaccgcgacc agagcctatc
    19201 tgttacagaa gaaaccagac accgtatttt aaccgttgct gaagagctgg gctacaccaa
    19261 gcacctcaag acaggcgagt cccataaacc caagcaaaag attgccatta ttcaatgggt
    19321 cagcgaacaa ggggagctag acgacctcta ctactaccag attcgcctag gaatagaaaa
    19381 aagagcccaa gagttggact acgatatctt gcgctatttt aatgaccacc cttttaccct
    19441 aagcgaggaa gtgattggga ttctctgcat cggaaaattt agccgagctc agatttctgc
    19501 ctttgaagaa taccaaaagc ctcttgtatt tctagacagc gatacacttt ccctgggaca
    19561 tacctgtatt atcacggatt tttacactgc tatgaaacag gttgtcgatt atttcctcag
    19621 tcaagggatg gaccgcatcg ggattctaac agggcttgag gaaacaacag accaagaaga
    19681 aatcattcag gacaagcgtc tagaaaactt caaaaactac agtcaagcga ggggaatcta
    19741 tcatgatgaa ctggtctttc aaggaagatt tactgcccag tctggctatg acttaatgaa
    19801 ggaggccatt cagagcttgg gagaccaact tccgccagca tttttcgcag ccagcgatag
    19861 tttagctatc ggtgccctcc gtgccctcca agaagctgga atcagcctgc cagatcgcgt
    19921 cagcctcatt tcattcaacg acaccagtct gaccaaacag gtctatcctc ccctctctag
    19981 tattacagtt tatactgaag aaatggggcg agcaggtatg gatattctta acaaggaagt
    20041 cctccacggt cgcaaaattc ctagcctgac catgctggga accagactga cattaagaga
    20101 aagtacccta aatcaagaat aggataacat aaaaaacgaa tagagttcta aaactcctat
    20161 tcgtttttta ttcgattaca atcatagact taattgtctt acgttcatcc atatctttat
    20221 aggcttggtc gatatcttcc agtttataac ttgaagtaaa gacgcgacct ggattgatat
    20281 caccatcaag gacggctttt agtaaaaatt gcttatcgta tgttgtagca gaagctgccc
    20341 cacctgctac agagatattt tgcataaatg tcgaaccaag agcacgatta ttatagtgtg
    20401 ggactcctac aaaacccata cgccctccat tatgaagaac acctagcgcc tgttctatag
    20461 cagcctccgt accaacacat tcaagtgctg cgtctgctcc tccgccgagg atttcacgca
    20521 ccttggtaat tccttcttga ccacgttctg caacaacagc tgtcgcacct gactccatag
    20581 ccatcttttg acggtcttca tgacggctca taaggataat ttgtgatgct ccacgcatct
    20641 tagccgcgat gacagcacat tgaccaacag ccccatcacc gataacaaca accttgtccc
    20701 ctttttgaac atttgcaaca cgcgctgcat gatagcctgt cggcatgaca tctgcaagag
    20761 tcaaaaggga cttgagcatc ccttctgtat aatcagaagg ttgaccaggg attttaacca
    20821 gcgcccagtt tgcatagtgg aagcgaatat attctgcctg aaaatcaccc cccaaattat
    20881 taccaatatg attgtcgcaa gaaccgtcaa atccagcaag acaggcatca cactcaccac
    20941 atccatgtgt aaaagggaca atcacaaaat cacctggttt caccgtcgta atggcttccc
    21001 cagcttcttc aacaatccca atcgcttcgt gtccactatt tttatgtcca gctttcgttt
    21061 ctggattacg gtacctccat aaatctgaac cacaaacgca cgcacgaacc acacgaataa
    21121 tcacatcatc cgcttctatt atttgcggac gttcaatgct agcaagtcca acctgacctg
    21181 cctttgtata tactgctgat ttcatttaaa attttccttc cttataaagt ttaattttga
    21241 gatttaaacg atttaaagct gtctgtagct gagacaagcg ctcctctaat ttttgctttt
    21301 cctcttctaa aataccaagc ctctcctctc tcgtttcatc tcccttttgg tagagcgaca
    21361 tatagtcaac taaactatct acagagacac ccgccgaacg aaaacactta ataaattcca
    21421 gcgcttcgat atcctgatct tgaaaatcac gaatcccagt agcagtacga gtaatcggtg
    21481 gcacaagacc aacccgttca taataccgaa tcgtatccgc tgaaattccc aacaaatcac
    21541 tggcagattt aatattcaca cgctaacctc ctctcatttc attcactgaa ttcacgctat
    21601 tctaactcac ttatcttatt atataacttt gagttgactc caagtcaagc attttgtatc
    21661 taatcgaaaa atttttatca ttaacaatca tatatctgga gtttatacaa tgctcttctc
    21721 ttaccaattt ttgaaatatt taaataataa agaaattact ctattccaaa taagatagta
    21781 cccaagtatt tttcactttt ttctaatgtt gatgctcata actccgttcc aagtcacaca
    21841 cctttcgctt atgggtttgg tgagtttcta ggtcagcatc aatttcaatg gtaatatttt
    21901 gaaaaccaca atcttttagg aaaattcgaa tagactcttt acaagtttcc atatgttcca
    21961 tttcttttag acaaacatgg acaatggcat ttttttccaa agcatccata gtccagagat
    22021 taagctgatt aaggctggcc acattgtcca atcgctccag gccactcttt acttgcttga
    22081 tatcaagacc ttctggcaca gcatccaaga aaatcttgag tgtagaccaa aaacgtggaa
    22141 gggcttttga aagaataaag aaagaaatga caagggacaa aagaggatct aggatatacc
    22201 agtccgtaaa tcgaagaaca atcgccatca ggataacagc tacccaccct agcgtatctt
    22261 ccagaaaatg cagactcaga atagactcat tctttgtctt tcccttacca accaccagac
    22321 tcgctaacag attgatagta atcgcaataa ttcctaacca gagaatcccc tcatcattga
    22381 ctggttgcgg atgcaaaatc ttcgtgacat tttccaaaat gactagaaca gagcccgtta
    22441 cgagaatcac agctgttacc aaggctccta gcaggctaaa ccgcttatag cccaaggtgt
    22501 actgattgtc ttcttcacga ttggagattg tttctagaaa agctgatatt ccaattgcaa
    22561 tcgcatctcc caagtcatgc acagagtcag caagaacagc gctagaacca aatactccac
    22621 ctgcaataaa ctcaacaatg gcataagtca aatttaagaa aaaagccacc caaacagcat
    22681 attttgcctt catatttctc attcctttgt tataatagat ttatgaacac cttgttcatt
    22741 atcattatcc actaaaactc aaagaaagga tagaagcaaa acgtcagctt tattcagttc
    22801 tgaacaattt gccttaagtg tccatatgac taacattgac cgccgtatca gcaaaaccaa
    22861 aaaagccatc tatcaagctt ttatacaact tttgaatgct aagggctacg aggccactac
    22921 tgttcaggat atcattgatc tcgcagatgt gggacgatcc accttttact gtcactatga
    22981 gagtaaggag cttcttctgg accagctctg ccgctacctc ttccatcatc tttttgaaag
    23041 agagcaagcc atttcaaccg aggactacct cgcccacctc tttctccatt ttcataaaaa
    23101 ccaagaccat atcaccagtc tgctattttc caaaaatgac tacttcctcc gtcaactcca
    23161 taaagagcta gaacaccatg tctattccgt gctagctgat aatttaaaag aagcccaccc
    23221 taatctgcct acttcttacc tccaacactt ggtcatgtcc aactttatcg agacattgac
    23281 ctggtggctc aaaaaaggtc aagatttcac agaccaggaa gttgtccaat tttatctaga
    23341 ccttctcatt cctaaaaatt gaatatagag taaagcttca gttgtcttat ttctaggtta
    23401 ctgagttttt tatcttttca acaacaaaag aggacccgcc gatcctcttt ttcatactat
    23461 aaatccttga ttatcaacta tatctgtttt aatcgaaatc tcaaaacagc actttcaaac
    23521 atcttttcct agttaagtaa atcagtattc tgcttagctg ccttgctcca ttgataccaa
    23581 ccaactagac tgttaatgag ataaattaga tatttccctt gaatttgcag gctttctccc
    23641 caccagagat agattgaaaa gacattggta gccgcccaga atatccactg ttcacggtaa
    23701 acagctgtca tgaggatttg ccctacccca ttggttgcat ctgtgattga atcacgatag
    23761 ggacgattgg caccaataga ctgataaatg aagccaaagg ccaaccacca aagcacacta
    23821 atggaaagat actttgtcca gcccttgccg tccagtttac gcgcgacaaa ctcctgcttt
    23881 tccttcttaa actgtgcctg ataaatccaa actagaagtc caattggctg catgactgtg
    23941 aagtaaagtg tcgtcagcac ctcaccataa aagcctttct gtagggccaa aataaggtaa
    24001 ataacagagt taatcaagcc aaaaagataa ttacttgctc gaccttccga tacaaagatt
    24061 acacagataa tccctgtcaa gctacaaatc atcccaatcc agtcaacaat acgatgttcg
    24121 taaaccaact ccagccagag aggaaaactt cctaaaacca gcaaataaat ccactgggca
    24181 aaactacgat gggcaaagag gtcatcccag atagccttca tagttcctga aaatcctaaa
    24241 tcagccatag ccgcaaccat acgacggtaa ccacctgaca tttcacctag ggttgttttg
    24301 atattttcaa ttttcttttg caaataagta tgcatcattt ctccttttgt ttttaaagag
    24361 ccgtgtctgg atagactttc ggacgcaacg ctctattaga taatgaactg cctatacaca
    24421 agatttctaa ccttagtcga catgagctga aacctcttat ttgttaagta gttcacaaaa
    24481 tattatacac ctattttatg aatagtcaac tgtctttaca gtaaaatttt agaaaatcat
    24541 gaaaattttc tctttctttc cattttaagt gacattcagt cattctcaca tcaaaaaagc
    24601 ccagacgaaa ttgtctgagc attcttttat ctagtcgttt aaggaagttg agttcagtat
    24661 gtttaaagtc tctgtcccat catttcttca acaaaccttg ttcttggaga aactccttgg
    24721 ctacttgctt tgctgacttg ccttcaacac cgacttggta gttgagctgg ctcatctggc
    24781 tttctgtaat cttaccagcc aatgtattaa gaactctttc caactctggg tgtttcttga
    24841 gaagagcttc tttcatgagt ggagcccctt gataaggtgg gaagagttgc ttgtcatctt
    24901 ccaagacctg taaatcataa cgctccaatt ccgcatcagt cgaataggca tccgtgattt
    24961 gaatatcccc tgactgaatt gcctgataac gaagagctgg ctgcatagtc gccacattga
    25021 gattgagacc atacattgat tgcaagccct tatttccatc ttcacggtcg ttaaactcaa
    25081 gcgtaaagcc tgccttcagc tgcccttcca cttttttcaa atccgaaatg gtcttcaagc
    25141 catattcttg agcaatcttt ttcggaacag ctacagcata ggtattttga taagacatag
    25201 gtttgagata ggctagatga tcctgcttgg caatgccatc acgcgccact tgataaacct
    25261 gctctggctc atgactcacc ttgggtgatg gttgaagcaa actttcagtc accgtaccgg
    25321 taaattcagg ataaatgtca atatcacctt ttttcagagc ttcataaagg aaacttgtcg
    25381 tcccaaaatt cggtttaaca gtcgcagtca tgctggtatt ttcttcaatc agcaatttat
    25441 acatattagc caaaatttct ggttctgggc ctattttccc agcaataatc aagttttcct
    25501 tctctttttg agccaaaagg gctggactat aagacagacc cagtaataaa gccaccaagg
    25561 caaaaccaga gaaaatcgtc cgcaatttcg ctttttccat tacttttagt aggaagttaa
    25621 aggcaatagc tagcactgca gaagaaaggg ccccaatcaa aatcaaactg gcattattac
    25681 ggtcaattcc caacaggata aaggaaccca gtccccctgc cccaatcaag gtcgccaagg
    25741 tagccgtacc gataatcaag accgctgccg tccgaattcc agacatgata acaggcatgg
    25801 caagtggaat ttcaaacttc ttgagacgct cccatctggt catcccaaag gcaatcccag
    25861 cctcttgaag actcggatca attcccttca gcccagtaat agtattttgt aaaatcggaa
    25921 aaatcgcata aatcactaga gctgtcaaag ccggcaaggt cccaattccc atcaaaggga
    25981 taaagagccc caacaaggcc agagacggga tggtctggaa aatacctgca atctgcaaga
    26041 cccagtcggc cagcttctca tgatagcgaa gaaaaacagc caagggaatc gcaagcaaaa
    26101 tagctagtaa caaggtcaaa agtgacaact gcaaatgttg agatagagct gtcaaccaat
    26161 cactaaaacg atcctgaaaa gttgcaatta aattagtcat gaacactacc tccaaacaag
    26221 tctgctacaa agtctgttgc aggcactttt aaaattgtct cgggattcgc tacctggcga
    26281 atttctccat cctgcaagac agcaatacgg tccgccaact tcaaggcttc atccgtatca
    26341 tgggttacaa aaatcgttgt catcccaaac tctttatgca attcttttgt cagaacctgc
    26401 aactgttttc tcgaaatagc atccaaggcc gaaaagggtt catccatgag gaaaatcttg
    26461 ggctgaccaa tcatagctcg gacaataccg acccgttgct gttctccacc agataattca
    26521 ctaggtaagc gatgcccata ctcggctact ggtaaaccaa ccttagccaa aagctcttct
    26581 gttttcttcg taatttcttc cttgctccac cccttcattt caggaatgag agcaatattt
    26641 tccgcaactg ttagatttgg aaaaagagca atagcctgta aaacataacc agtagaaaga
    26701 cgaagttcac gctcatcata gtctttgatg cgcttcccat ccatataaat atttccatca
    26761 gttggttcca aaagacggtt aatcatcttg agcatggtcg tcttacctga cccagaaggc
    26821 cctactaaaa ccataaattc cccatcctca atctgtaagt tgacatctct caagacatcc
    26881 ttttctgtgt agcgcagtgc tacatttttg tattcaatca ttctttgtcc tcaatttaaa
    26941 acttccctcg attagtcaag tcttctacct taggcatgac ttccttatta tcccaatgct
    27001 ccacaatttt cccgttctct aaacggaaga tatcgtactg ggcataagca acgccatcaa
    27061 tctgagtctg accatagcta accacatagt ttccttgtcc taagagttgg aaaacaaagt
    27121 caaaagtgac actatattca gccacatagt ttttataagc agcacttcct tgtccaatct
    27181 catgattatg ctgaatcaaa tcgtctgcca cataatcact ccactgctct agctccccat
    27241 tttggaaaat ttctgtcaag aaacggcgaa ccagtttttt attttctgct ttcttgtcca
    27301 aatccttgat ttcaaaatct ccaaaaattt gatctagttg atcattttca ggtgttcgat
    27361 agtagtcaat gacatcccaa tgctcaacaa tacgaccatt ctcatccgca cggaaagtat
    27421 ccgtcgtcac ccattgagct tctccaccat tcagatattg atgaacatga acaaagacca
    27481 gattgccatc ctcaatggtg cggacaatct taatctgacg ctctggatga cgctcaaaga
    27541 aatctgcaaa gaaggctgca aatccttctt tcccgtcagg aacacctgtc gaatgttgga
    27601 tataggtatc ccctacagac tgggcttgag cctcagcaac tcgtccgtct tgaatggcat
    27661 ggatgtatag gttgtgagca tttttcactt gttgtgacat attctaaacc tcatttccct
    27721 tctctttcaa attcgccaaa attctttctt gaaaaccttc aaattggtga atttcttcct
    27781 ctgaaaatcc tttgtaacag atagtatcca atctctgact gacacgttgc ccaacttctt
    27841 tctgggactt gcccaactct gttaaaacta aatacttctt acgcttatct tctccacacg
    27901 gactgacagt tacaagattt tgttcctcta gattttttat catagtcgtc agcgtattat
    27961 tagcaagccc agtcgcaagc gcaatgtctg tcgccgtagc acagcctgtc tcactattcc
    28021 ataaaaccgc taaaatcttt ccctgctcac tccgataaag agagtcaggg tcttgtctta
    28081 gtaacttttg aaaaatccgc ccattcaaca aacgaatatg atgggctacc aaatgacaat
    28141 atttcatgaa acctccaatt tatttctata tcgaaataga taaagtgatt ataacactca
    28201 tcattccata tgtcaactat ttcgatttag aaataatttt tgataattat ttacatcacc
    28261 atactccgga tcaactaact tttaaggaga gtttctaaac tccttcgtcc tccagtctac
    28321 aaaagccttt catccgtact atcctatatt ttatgagggg acacattttt cctatcagac
    28381 catttatttt aaagatagaa gtaaatcata attgcttcca tctgttcttt tatagtatat
    28441 tgaagttgaa ctaggacact gtatcttcta aaacattgat aaaaagcgac ttgaatttcc
    28501 caatcaattt gttcgtattt atagcatttc gaaactggaa taggacacca tgactgctaa
    28561 aagatttcta taaattcatt tgatttccct aatcaatttg ttcatatctt atttcattcc
    28621 gctataattt caccttaccc tatctttttc gtagcaccct tcaaacagcc tatcccctac
    28681 cgtttgacga ttcctcactt cactccactt ccattacaga agtttcttca ctactatggt
    28741 ctcggctgac ttctcatgat tccttgttac tactatttga acgctcacga gataggtctt
    28801 ataaaaaatg ctttgatcca caatggaatc aaagcatttt aaaaagttcc tcatacataa
    28861 gcgcagaagt cgcagttcct ctgtacttgg cttcttctct tttgataaag cgagccaagt
    28921 tgagcaactc aggtgctgga tgtttgggat ttaggagcaa ttcacgattg accaggcctg
    28981 agagacgaac tgcctgcaat tgctcatttg tagtaggcag ttttttagta gtctctagga
    29041 gagcagcaac taaatcttca ctcaaatcat gtcgagcatg attgtaaaga tcttttataa
    29101 ggctttctag gtttggttct accatcccta ccacctccct tatggtttaa taatgtttaa
    29161 tcaaatcaac cgttgaacga tccaatttct tcaccaaggc ttgtaagaaa gcttgcgctt
    29221 ctaggaagtc atccattgca tagagggttt ggtgagaatg gatataacga gcgcagacac
    29281 cgatagttgt tgatgggaca ccaccatttt tcagatgagc tgcacctgca tctgttccgc
    29341 ctttaccaca gtagtattgg tacttgatac cagcttcttc agccgttgtc aaaaggaaat
    29401 ccttcatccc tggaagaagc aagtgacctg gatcatagaa acgaatcaag gttccatctc
    29461 caatcttgcc ttgaccaccg tagacatcac ctgctggtga gcaatcaact gcgaggaaga
    29521 cttctgggtc aaacttggtt gtagaggtat gagcgccacg cagaccaact tcttcttgga
    29581 cgttagaacc cagatagagt tcattgccga gtttttgacc tgataaagct tcagctagct
    29641 cgcttaccat gaggacaccg tagcggttat cccaagcttt tgagatgata tttttttcat
    29701 tggctgtcaa aattgcagaa ctatctggta cgatggtatc accaggacgg atgccaaaac
    29761 tttctgcctc agccttgtcc gcaaaaccac catcaaaaac gatatcggca atggctggca
    29821 tggttggtcc cccctttcca cgagtcaaat gcggaggaac agaacctgaa atcacaggaa
    29881 tttcatgacc atcacgagtc aagagtttga aacgttggct gctaaccacc atggggttcc
    29941 agccaccgat ttctacgaca cggaaggtac catctggctt gatttcgctg accataaaac
    30001 caacttcgtc catatgagaa gcgaccaaga cgcgcggtgc atccacagct tctgaatgtt
    30061 tgataccaaa aataccaccc aagccatctg tcaccacttc atccacatgc ggtgtcaact
    30121 tttcacgaag ataagcacgg acaggcgctt catgacctga gactgcagca agttctgtta
    30181 cttctttaat ttttgaaaat aatgttgtca tttcagttcc ttctttcttt catccatttt
    30241 accacttttt ataggagaag gatagtggga aggtggattt ctaagttagt atcttagtcc
    30301 tgctctatct tagaaaagga tagtattctc ttgcatgtag tgcaaaatct agtaaacatt
    30361 ccaaaattaa ctcgaatatt tatttccaaa caaaaaaaca atacaccatc aaagttgttt
    30421 agatttttta tgaaatttac agaaaatagt tgacttccct ttcttctttc tttaaatata
    30481 tagttggttg agtttggaat agtacgctgt agctgctaaa acatttctag aaattaattt
    30541 gactttccta atagagttgt tcatatctta tttcaattta ctatagtaca aaactagaaa
    30601 aggaaaaaat catgaccaaa acaattcttg ttacaggtgg gactagctac attggctccc
    30661 ataccgttaa agctctttta aatgctggct ataaggtgca tgtcctcgat aatctctcca
    30721 caggaaatcg agcggctgtg gatagtcgtg ctagctttaa agaactggat gtttatgatg
    30781 ctagcgcctt aaaggcttac ttagaagaaa atcagattga tgctgtcctc cattgtgcag
    30841 gtgaaattgt tgtgagcgaa agtattgaaa atccaagtaa atacttcact gccaacgttg
    30901 ctggtaggaa ccaagttctc aaagtcttat ctgaagttgg cattcaaaaa atcatgttct
    30961 cttcgactgc ttctctctat ggaaataact gtattgacaa gccggtgact gaagataccc
    31021 tgctcgaccc tgtcaaccct tatgcagaga caaaactgat gggcgaacga atgatttact
    31081 ggatggccaa tcgctacgac tggaaatatg ttattttccg ttactttaat gttgctgggg
    31141 ctgaaatgga tgcttcaaac ggtctgcgtg tgaaaaatcc aactcacatc attccaaata
    31201 tcaacaagac cgcattggga caaaatgata gcttaaaaat atttggagat gactacgata
    31261 cacatgacgg ttcatgtatt cgagattaca tttatgtctt ggaccttcat aggctcatgt
    31321 taaaggaatg aactacctat ttcaagaaga cagttcttct
//
LOCUS       DAVAAL010000011        30526 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVAAL010000011 DAVAAL010000000
VERSION     DAVAAL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.011"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            653..967
                     /locus_tag="VQM60_000377"
     CDS             653..967
                     /locus_tag="VQM60_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HET1080275.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            983..1369
                     /locus_tag="VQM60_000378"
     CDS             983..1369
                     /locus_tag="VQM60_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HET1080276.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1398..2783
                     /locus_tag="VQM60_000379"
     CDS             1398..2783
                     /locus_tag="VQM60_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET1080277.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVQAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2786..2938
                     /locus_tag="VQM60_000380"
     CDS             2786..2938
                     /locus_tag="VQM60_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HET1080278.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2961..4223
                     /locus_tag="VQM60_000381"
     CDS             2961..4223
                     /locus_tag="VQM60_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HET1080279.1"
                     /translation="MEGFLLNEQTWLQQLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLFEALERFNPDAPLEMLFDYVRIRFPTTDVQHVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDALVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKKGYLSTIPVDRYPKKRYNGR"
     gene            4168..4380
                     /locus_tag="VQM60_000382"
     CDS             4168..4380
                     /locus_tag="VQM60_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HET1080280.1"
                     /translation="MCQPYQLTDIQKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKE
                     VMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            4429..4512
                     /locus_tag="VQM60_000383"
     CDS             4429..4512
                     /locus_tag="VQM60_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HET1080281.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            4637..5374
                     /gene="erm(B)"
                     /locus_tag="VQM60_000384"
     CDS             4637..5374
                     /gene="erm(B)"
                     /locus_tag="VQM60_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HET1080282.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            complement(5631..6878)
                     /locus_tag="VQM60_000385"
     CDS             complement(5631..6878)
                     /locus_tag="VQM60_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HET1080283.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            7058..7279
                     /locus_tag="VQM60_000386"
     CDS             7058..7279
                     /locus_tag="VQM60_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080284.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            7396..7893
                     /locus_tag="VQM60_000387"
     CDS             7396..7893
                     /locus_tag="VQM60_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HET1080285.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            7868..8374
                     /locus_tag="VQM60_000388"
     CDS             7868..8374
                     /locus_tag="VQM60_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HET1080286.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            8358..10805
                     /locus_tag="VQM60_000389"
     CDS             8358..10805
                     /locus_tag="VQM60_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET1080287.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            10808..12985
                     /locus_tag="VQM60_000390"
     CDS             10808..12985
                     /locus_tag="VQM60_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET1080288.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKAERASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            12982..13983
                     /locus_tag="VQM60_000391"
     CDS             12982..13983
                     /locus_tag="VQM60_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HET1080289.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            13980..14912
                     /locus_tag="VQM60_000392"
     CDS             13980..14912
                     /locus_tag="VQM60_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HET1080290.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            15157..15273
                     /locus_tag="VQM60_000393"
     CDS             15157..15273
                     /locus_tag="VQM60_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HET1080291.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            15289..17208
                     /gene="tet(M)"
                     /locus_tag="VQM60_000394"
     CDS             15289..17208
                     /gene="tet(M)"
                     /locus_tag="VQM60_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HET1080292.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            17572..17817
                     /locus_tag="VQM60_000395"
     CDS             17572..17817
                     /locus_tag="VQM60_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080293.1"
                     /translation="MRQIGVSYSGFVDESYTLLSLFDDVEQIEKDNRLQTAIDVVREQ
                     FGFLAIQKGTVLTEGSRNIERSKLIGGHSAGGLEGLK"
     gene            17814..18149
                     /locus_tag="VQM60_000396"
     CDS             17814..18149
                     /locus_tag="VQM60_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imsC-like protein"
                     /protein_id="HET1080294.1"
                     /translation="MKQEKNTVQFSEIRSKGCNDIEMLERFLHGIVETATSKLRQRKL
                     KTTEISIRLVHAKSENRLPLEFTFSIKPTSSSVIIYTEVINRFKECYTGGGIQGFTIQ
                     FDKNTLASA"
     gene            18162..18530
                     /locus_tag="VQM60_000397"
     CDS             18162..18530
                     /locus_tag="VQM60_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080295.1"
                     /translation="MIDRSYLPFQSAREYQDTKMQKWMGFFLSEHASALSDDTNKVTY
                     MSDLSLEKKLLLLSQVYAGQLRTRIQVIEKNKRVSYTGTIPSLTKDFILIKTTTGHIN
                     LKLKDIISIELVEEVLYESA"
     gene            18517..18816
                     /locus_tag="VQM60_000398"
     CDS             18517..18816
                     /locus_tag="VQM60_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080296.1"
                     /translation="MNQLEFQRNHLQMDYYSESYQDFERDFYRYSNMNIPLTFLTDDI
                     LKTMATSRKNYFVLNKEKSRDNRDHFFIFEVSTVDENPLIYHYTYKKTTIYLAEK"
     gene            complement(18934..20397)
                     /gene="msr(D)"
                     /locus_tag="VQM60_000399"
     CDS             complement(18934..20397)
                     /gene="msr(D)"
                     /locus_tag="VQM60_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(D)"
                     /protein_id="HET1080297.1"
                     /translation="MELILKAKDIRVEFKGRDVLDINELEVYDYDRIGLVGANGAGKS
                     TLLRVLLGELTPPGCKMNRLGELAYIPQLDEVTLQEEKDFALVGKLGVEQLNIQTMSG
                     GEETRLKIAQALSAQVHGILADEPTSHLDREGIDFLIGQLKYFTGALLVISHDRYFLD
                     EIVDKIWELKDGKITEYWGNYSDYLRQKEEERKSQAAEYEQFIAERARLERAAEEKRK
                     QARKIEQKAKGSSKKKSTEDGGRLAHQKSIGSKEKKMYNAAKTLEHRIAALGKVEAPE
                     GIRRIRFRQSKALELHNPYPIVGAEINKVFGDKALFENASFQIPLGAKVALTGGNGIG
                     KTTLIQMILNHEEGISISPKAKIGYFAQNGYKYNSNQNVMEFMQKDCDYNISEIRSVL
                     ASMGFKQNDIGKSLSVLSGGEIIKLLLAKMLMGRYNILIMDEPSNFLDIPSLEALEIL
                     MKEYTGTIVFITHDKRLLENVADVVYEIRDKKINLKH"
     gene            complement(20517..21734)
                     /gene="mef(A)"
                     /locus_tag="VQM60_000400"
     CDS             complement(20517..21734)
                     /gene="mef(A)"
                     /locus_tag="VQM60_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HET1080298.1"
                     /translation="MEKYNNWKRKFYAIWAGQAVSLITSAILQMAIIFYLTEKTGSAM
                     VLSMASLVGFLPYAILGPAIGVLVDRHDRKKIMIGADLIIAAAGAVLAIVAFCMELPV
                     WMIMIVLFIRSIGTAFHTPALNAVTPLLVPEEQLTKCAGYSQSLQSISYIVSPAVAAL
                     LYSVWDLNAIIAIDVLGAVIASITVAIVRIPKLGNQVQSLEPNFIREMKEGVVVLRQN
                     KGLFALLLLGTLYTFVYMPINALFPLISMEHFNGTPVHISITEISFAFGMLAGGLLLG
                     RLGGFEKHVLLITSSFFIMGTSLAVSGILPPNGFVIFVVCCAIMGLSVPFYSGVQTAL
                     FQEKIKPEYLGRVFSLIGSIMSLAMPIGLILSGFFADKIGVNHWFLLSGILIIGIAIV
                     CQMITEVRKLDLK"
     gene            complement(22120..22290)
                     /locus_tag="VQM60_000401"
     CDS             complement(22120..22290)
                     /locus_tag="VQM60_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HET1080299.1"
                     /translation="MKYFEVELENPDEFLKLQTEDFVKANRLLLRKIIQSVTVYEENF
                     VISFKSGIELEV"
     gene            22812..23003
                     /locus_tag="VQM60_000402"
     CDS             22812..23003
                     /locus_tag="VQM60_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HET1080300.1"
                     /translation="MTSKLTASTCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIE
                     IKQFKVTVITEPDAKTQSR"
     gene            complement(23063..23416)
                     /locus_tag="VQM60_000403"
     CDS             complement(23063..23416)
                     /locus_tag="VQM60_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET1080301.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            23921..24343
                     /locus_tag="VQM60_000404"
     CDS             23921..24343
                     /locus_tag="VQM60_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET1080302.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            24340..24570
                     /locus_tag="VQM60_000405"
     CDS             24340..24570
                     /locus_tag="VQM60_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET1080303.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            25031..25234
                     /locus_tag="VQM60_000406"
     CDS             25031..25234
                     /locus_tag="VQM60_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HET1080304.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            25316..26533
                     /locus_tag="VQM60_000407"
     CDS             25316..26533
                     /locus_tag="VQM60_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET1080305.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            27424..29439
                     /locus_tag="VQM60_000408"
     CDS             27424..29439
                     /locus_tag="VQM60_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET1080306.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTPVFSYTTFGNGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGNLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFGALTLISQISQLRSSGIRLISGEKRWFIFLRPVGEDLKGIAVGFSLAGVLTI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNFITSTEWYDY
                     SPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEDHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            29441..30078
                     /locus_tag="VQM60_000409"
                     /pseudo
     CDS             29441..30078
                     /locus_tag="VQM60_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         9946 a         5505 c         6006 g         9069 t
ORIGIN      
        1 caaaatgcaa taaaaataat tgactgaata aactacatat gttagaataa aaacaaggaa
       61 aaagaaaggg gtttcattgc atgagaaaaa aacgtggaat taaaaaatta gttacatttg
      121 cattactagg tgtttttatg tttagtaata caattcctta ccaacagttt attcagaaga
      181 ataaacaatt ggaagttcga gtacaatcac aaaaggagtc caatggtctt gatgttggga
      241 aggctgatta gttgattaaa tgatgaaaat actaattaac tatatggtat ttctgtttta
      301 tctaattttt aattagaaaa atagcataaa aatctagtta tccgcataaa aactggactt
      361 atcacacttt atcaaggtca aaaccactca atttactact aatttactac ttatgaatga
      421 gctttgatac gacgatttat ccttgaaaag tgaagatata aagatacttc caataaaatt
      481 tgaatattta ataggtagac acttcaaaaa atgaggtgtc tattttttta cccgattttg
      541 aaaggaagtg aacttatgaa aacaaaaaat caagaatcaa aaggtcgttc cccactcttt
      601 aagaccatca aacattcatt cagccaataa aaaagaaagg ataggtaaaa atatggaact
      661 taaatttgtg attcccaaca tggaaaaaac attcggcaat ttagaatttg ctggcgagga
      721 taaagtcgtt cagcgaagaa tcaacggacg gctaactgtc ttatcaagaa gctataatct
      781 ctattctgat gttcaaagag cagatgatat tgtggtggtg cttcctgctg aagctggcga
      841 aaaacatttc ggctttgagg aacgtgtgaa gttagtcaat ccacgtatta ccgcagaggg
      901 ctacaaaatc ggcactcgtg gttttacaaa ttacctttta catgctgacg acatgataaa
      961 agaataaaga aagagaggaa aaatgatgag attagcaaat ggcattgtat tagataaaga
     1021 cacgactttt ggagaattga aattctctgc tctacgtcgt gaagtgagaa tccaaaatga
     1081 agacgggtcg gtttcagatg aaatcaagga acgtacctat gacttaaaat ccaaaggaca
     1141 aggacgcatg attcaagtaa gtattcctgc cagcgtgcct ttgaaagagt ttgattataa
     1201 cgcacgggtg gaacttatca atcccattgc ggacaccgtt gctactgcca cctatcaagg
     1261 agcagatgtt gactggtata tcaaggcaga cgatattgtg ctgacaaagg attctagttc
     1321 attcaaagct caaccacaag caaagaaaga accgacacaa gacaaatagt cgctaggtag
     1381 aaaggagact ttttcgcatg aaacagcgtg gtaaaaggat tcgcccatct ggtaaagatt
     1441 tagtctttca ttttacgata gcgtcactcc tgcctgtttt cctgctggtt gtcggactgt
     1501 ttcatgtgaa gacaatccag cagatcaact ggcaggattt taacctatca caagcagata
     1561 agattgacat tccctattta attatcagtt tcagtgtcgc aattcttatc tgcttgctgg
     1621 tagcgtttgt attcaaacgg gttcgctatg atacggttaa acaactttac caccgtcaaa
     1681 aactggcaaa gatgatactt gaaaacaagt ggtatgaatc tgaacaggtc aaaacagagg
     1741 gtttctttaa agatagtgct ggtcgtacaa aggaaaagat aacctacttc cctaaaatgt
     1801 attatcgact taaaaatggc ttgatacaga tacgggtgga aatcacgctg ggaaaatatc
     1861 aagaccaact cttacacttg gaaaagaaat tagagagtgg cttgtactgt gagctgacgg
     1921 ataaagagtt aaaggattcc tatgtggaat atactttgct ctatgacacc atagccagtc
     1981 gtatttctat tgatgaagta caagctaaag atggtaaact tcgcttaatg aaaaacgtat
     2041 ggtgggaata tgataagctc cctcatatgt tgattgctgg tggtacaggt ggcggtaaaa
     2101 cttactttat actgacactg attgaagcct tgcttcatac agattcaaaa ctgtatattc
     2161 ttgacccgaa aaatgctgac cttgcggact taggttctgt gatggcaaat gtctactata
     2221 gaaaagaaga cttgctttct tgcattgaaa cattctatga agaaatgatg aaacgtagtg
     2281 aggaaatgaa gcagatgaag aactataaga ctggcaaaaa ttatgcttac ttaggtctcc
     2341 cggcacactt cttaatcttt gatgaatacg tcgctttcat ggaaatgctg ggaacaaaag
     2401 aaaacaccgc agttatgaat aagctgaaac agattgtcat gttaggtcgt caagctggct
     2461 tctttctaat actggcttgt caacgtccag acgcaaaata tttaggcgac ggaatccgtg
     2521 atcagtttaa tttcagagtg gctttaggtc gtatgtctga aatgggctat ggcatgatgt
     2581 ttggcagtga cgtacaaaag gatttcttct taaagcgaat caaaggtcgt ggctatgttg
     2641 atgtaggaac aagtgtcata tcagagtttt atactcccct tgtaccaaaa ggatatgatt
     2701 tcttggagga aattaaaaag ttatccaaca gcagacagtc cacgcaggcg acgtgcgaag
     2761 cggaagtcgc aggtgtggac tgatcttgct ggctggtgtg gcaatagcca cgccagcact
     2821 taaccccccg tatctaacag gggggtacaa atcgacagga aacagtcaaa aaaacattag
     2881 aaaatccttt ggttacaagg gatttacaaa atttcagcgt atgtcaaatg ggctttaaaa
     2941 gttgacatac gcctttttga ttggagggat ttttactgaa tgaacaaact tggttacagc
     3001 agttaaaaga aaaacgcttg gcttatggac tatctcaaaa tcgtttagcg gttgcgactg
     3061 gtattacaag gcagtatcta agcgatattg aaacaggaaa agtcaagcca tcagaggatt
     3121 tacagcaatc tctttttgaa gctctggaac gcttcaatcc cgacgctccc cttgaaatgc
     3181 tctttgatta tgtaaggatt cgctttccca caacggacgt acagcatgtg gtcgaaaaca
     3241 tcttacaact gaaactgtcc tattttcttc atgaggacta tggtttctat tcttattcag
     3301 agcattatgc tttaggcgat atattcgttc tctgctccca cgaactggac aaaggagttc
     3361 tggtggaatt gaaaggtcgt gggtgtcggc aatttgaaag ctatcttctg gctcaacaaa
     3421 gaagctggta tgagttcttt atggacgctt tggtggctgg cggtgtgatg aaacgccttg
     3481 accttgccat taacgataag acagggattt tgaatatccc tgtactcact gaaaagtgcc
     3541 aacaggaaga atgtatctcc gtcttccgca gttttaaaag ctatcgcagt ggcgaactgg
     3601 tacgcaaaga ggaaaaggaa tgtatgggaa acaccctcta tatcggttca ttacaaagtg
     3661 aagtttattt ctgtatctat gaaaaggact acgagcagta caagaaaaat gatattccca
     3721 ttgaagacgc agaagtaaaa aaccgttttg agattcgatt gaaaaatgag cgtgcctatt
     3781 atgcagtccg tgatttactc gtctatgaca atccagagca taccgccttt aaaattatca
     3841 atcggtatat ccgttttgta gataaagacg attccaaacc tcgttctgat tggaaactga
     3901 atgaagaatg ggcttggttt attgggaaca atcgtgaacg attaaaacta accacaaaac
     3961 cagagcctta ctccttccaa aggacgctga actggctatc tcatcaagtt gccccgacct
     4021 taaaggttgc gattaaactt gatgaaatca accagacgca ggttgtaaaa gacattctcg
     4081 accatgcgaa actgacagac cgacacaagc agattttgaa gcaacagtca gtaaaagaac
     4141 aggacgtgat aacaacaaaa aaaggatatc tgtcaaccat accagttgac agatatccaa
     4201 aaaaaagata taatgggaga taagacggtt cgtgttcgtg ctgacttgca ccatatcata
     4261 aaaatcgaaa cagcaaagaa tggcggaaac gtaaaagaag ttatggaaat aagacttaga
     4321 agcaaactta agagtgtgtt gatagtgcat tatcttaaaa ttttgtataa taggaattga
     4381 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
     4441 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
     4501 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
     4561 cagataacta aaattacaaa caaatcgttt aacttctgta tttgtttata gatgtaatca
     4621 cttcaggaga gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
     4681 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
     4741 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
     4801 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac
     4861 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
     4921 taaaattgtt gggaatattc cttaccattt aagcacacaa attattaaaa aagtggtttt
     4981 tgaaagccgt gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
     5041 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
     5101 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
     5161 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
     5221 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
     5281 gaaacacgcc aaagtaaaca atttaagtac cattacttat gagcaagtat tgtctatttt
     5341 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     5401 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     5461 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     5521 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgggat atctgtcaac
     5581 tggtatggtt gactaaaaat acttcctacg aaaatgtagg gggtattttt ttacgaaaaa
     5641 atacaatcga ttcttaaaaa gaaaaatttt tgattggcaa aaccataaca agttcgtttt
     5701 agggttttga ttttgcgatt gatgccttct aaaggaccat tagagtattc aaatttagcg
     5761 ctatttaaga catattttct gttttgacga agggtttgaa tagcagtatc catttctgta
     5821 ttggtttttt ggtagtctaa gatggttgac tctagtaatt cactattgcg ctcgtttagg
     5881 gctttcgtga tatcttggta agtttggtat acttcagcga acttggaaaa tttactagta
     5941 atgagatcaa cagcattttg gcgagtcata tattgtttaa cgccgcgaag aaaaactact
     6001 tcttcagggt gaagatcttc agctttttta tggaatagct tccaatgtga cttcataatt
     6061 ttatattctt ggctctgttt atcaagttgc tttaggatag agatacgaca attgtccaaa
     6121 gcgcgaccag ctaattgtac aaggtggaag cgatcaataa tgatattggc attagggaaa
     6181 aggcgataga taaaactttg atattgagca tttaaatcaa ttacaactga ttgaacgcat
     6241 tcgcgttcgg cttttgaata acgactttca aaataatcaa caatggtagg tgatagacga
     6301 tcctgtaact ttgtgacaat ttggtgggtt tcagcgtcac aacagataaa ggacatcaca
     6361 gacttaattg aacgaaactc gtcaaaacat agatgcttag gcaacttagc cacacgatag
     6421 tgtggttcca tgcgctctaa gattgttcga cgaacactgc taggagagca gtgacacatt
     6481 tcagcaataa gctgaccaga taagccttta cgagctaaaa gcatgatttg atttttgaga
     6541 tcactggata aggtttgatt ttctttggtt aaattagtaa tagcaccaaa agtagtatgg
     6601 catgatttac atttatagcg ttgtttacga agctctagtt catatcttct cccatttaaa
     6661 cttgccagtc gtacatgagt tttgcgaaag ccatccttat taactgtggg aaagccacag
     6721 ttacgacaac gattaatcgg ataagaaaga gtagctgtta ttagcgttat atactcttta
     6781 acagaatcgt tgttgtgttc agcttcttca acagaaataa ttttaatatt tttatcttta
     6841 attccaagaa tatttaggat agaatcatta tgggacattt gtttaacctt ctttcatgat
     6901 ttttgtggtg aattgattgt ataacgaggg gacagcaaat gtcctctttt ttgtataaaa
     6961 aaatctggca tggaatctct atccatacca gaaagtgtat accccaaaaa aataactcaa
     7021 atacaaattc attgaatata gagaggagaa catttttatg aattttggac aaaaccttta
     7081 taactggttt ctatcaaacg ctcaatcact ggtgctttta gcaatcgttg tgattggctt
     7141 gtatcttggc ttcaagcgtg agtttagcaa actgattggc tttttaatta ttgcgattat
     7201 tgcggttggc ttagtcttca acgctgctgg agtaaaagac attttactag agctattcaa
     7261 tcgcattatt ggtgcttaaa taaaaccgtt cttttgtgga atataagtgg ttttcttatg
     7321 ttccgcaaag gaatggtaca ccaaacgaag tgcggtaggg atttttgaat ctctacaaag
     7381 aaaggacgtg aatatatgga cgatatgcaa gtctatattg cgaatttagg caaatacaat
     7441 gagggcgaat tggtcggtgc gtggtttacc tttcccattg actttgagga agtcaaagag
     7501 aaaatcggct tgaatgatga atatgaggaa tacgccattc atgactacga gttacccttt
     7561 acggttgacg aatacacttc cattggcgaa ctcaatcgac tatgggaaat ggtatcggaa
     7621 ttacccgaag aattacaatc ggagctatct gctctgctca ctcatttttc aagcattgaa
     7681 gaactaagcg aacatcaaga ggatattatc attcattccg attgtgatga tatgtatgac
     7741 gtggcacgct actacattga agaaacgggt gctttaggcg aagtaccagc tagtcttcaa
     7801 aactatattg attatcaagc ctatggtcgg gatttagacc tttcaggaac gtttatctca
     7861 accaatcatg ggatttttga aatcgtctat taaatctgtc ggtacattac tactggcaga
     7921 ttttctattt tacggggtgg ctcaatcagc tacccctatt ttttatgaaa ggattgatta
     7981 catgaagaaa atacgaagct ataccagtat ctggtctgtg gaaaaggtac tgtattctat
     8041 caatgatttc agacttccgt ttcccataac ctttacgcaa atgacatggt ttgtcgtgtc
     8101 actctttgca gtgatgatac ttggcaactt gccccctctt tccatgatag agggagcatt
     8161 tctcaaatac tttgggattc ctgtggcttt cacatggttt atgtctacaa aaacttttga
     8221 tggtaaaaag ccttatggat ttttgaagtc tgtcattgct tatgcactgc gaccaaagct
     8281 gacctatgca ggaaaaaaag taacgcttgg cagaaaccag ccacaagaag ccattacagc
     8341 agttaggagt gaattttatg gcatatccaa ttaaatacat tgaaaacaat ctcgtctgga
     8401 ataaagacgg ggaatgttat gcttactatg agcttgttcc ttacaattac tcatttctaa
     8461 gtccagaaca gaaaatacaa gtgcatgatt ctttcagaca gcttatcgca caaaatcgtg
     8521 atggcaaaat tcatgcttta caaatcagta cagaatccag catacgttct gcacaagagc
     8581 gttccaaaaa tgaagtcact ggcaagctca aagcggttgc ctatgacaaa atcgaccaac
     8641 agacagacgc tttaatatcc atgattggcg aaaatcaagt gaactaccgt ttctttatcg
     8701 gctttaagtt gcttctcaac gatcaggagt tttctatgaa aagtcttacc gttgaagcaa
     8761 aaaatgcttt gtctgatttt gtctatgatg tgaaccataa gctgatgggc gattttgtta
     8821 gtatgagtaa tgatgaaatc ctgcgttttc agaagatgga aaagctctta gaaaataaaa
     8881 tctctcgtcg tttcaaaatc cgcaggttag ataaggacga cttcggctat ctgattgaac
     8941 acctttacgg acagacaggc actgcctatg aagagtatga gtaccatcta tcaaagaaaa
     9001 agctggataa tgaaacgctg attaaatact atgacttgat taagcctact cgctgtttgg
     9061 tggaagaaaa acagcgatat ttgaaaatcc agcaggaaga tgaaaccgtc tatgtagctt
     9121 actttaccat taacagcatt gtcggagaac tggacttccc gtcctctgaa atcttctact
     9181 accagcaaca gcaatttaca ttcccgattg atacgtcaat gaatgtggaa attgtagcga
     9241 atcgtaaagc cctatctact gtccgcaata aaaagaaaga actgaaagac ttggataacc
     9301 acgcatggca aagtgataat gaaaccagct ctaatgtggc ggaagctctg gaaagtgtga
     9361 acgagctgga aaccaattta gaccaaagca aggaatctat gtataagctg tcctatgttg
     9421 taagggtatc agcaaatgac cttgacgaac ttaaacgtcg ttgtaatgaa gtgaaagatt
     9481 tctatgatga tttgagcgtg aaactggtac gaccttttgg ggatatgctg ggcttacatg
     9541 aagaattttt acctgccagc aaaagatata tgaatgacta tattcaatac gtgacctctg
     9601 atttcctcgc tggtttaggt tttggtgcta ctcaaatgct gggtgaaaat gaggggattt
     9661 atgttggcta cagcttagat actggacgca atgtctatct gaaacctgct cttgccagtc
     9721 aaggggttaa gggttcagta accaatgcgt tagcgtctgc ctttgtcggt tcgctgggtg
     9781 gtggtaaatc ctttgcgaat aaccttatcg tctattatgc agtgctttat ggggcacaag
     9841 cagtgattgt agacccaaaa gcagaacgtg gcagatggaa agaaaccttg ccagagattt
     9901 cccatgaaat caatatcgtc actctgactt ctgatgagaa aaacaaaggc ttacttgacc
     9961 cttatgtgat tatgaaaaat cccaaagatt ctgaatcact ggctattgat attctgacat
    10021 tccttacggg gatttcctct cgtgatgggg aacgcttccc aatccttaga aaagccattc
    10081 gtgcagtaac caatagtgaa gtacgagggt tgatgaaagt gattgaggaa ttacgggttg
    10141 agaatacgcc actaagtacc agtatagccg accatatcga aagttttaca gactatgact
    10201 ttgcacattt attattcagt aatggttatg tggagcagtc tatcagctta gaaaaacaac
    10261 tgaacattat acaggttgcg gacttggtac ttcccgacaa ggaaacttcc tttgaggaat
    10321 ataccactat ggagctttta tccgttgcta tgctgattgt cattagtacc tttgctttag
    10381 actttatcca tacagaccga agcattttca agattgtaga tttagacgaa gcatggagct
    10441 ttttacaggt agcacaagga aaaacactat ctatgaagct ggttcgggct ggtcgtgcta
    10501 tgaacgctgg ggtatatttc gtgacccaaa atacagacga cctcttagat gaaaaactga
    10561 aaaataacct cggcttaaaa tttgcatttc gttccactga ccttaacgag attaaaaaga
    10621 ccttagcctt ttttggtgta gacccagagg acgaaaacaa tcagaagcga ttgcgtgatt
    10681 tggaaaacgg gcaatgcctt atcagtgatt tatatggtcg tgtcggtgtg atacagttcc
    10741 accctgtatt tgaagaactg ctccatgcct ttgataccag accacctgtg cgaaaagagg
    10801 tgtaaatgtg aaaccatcaa tagtaaacag aataaaatca aactggacgc tgaaacgtct
    10861 aggtaaagtg gcaatgacag tggctttcac acttgtgatt gccatttttc ttttagccat
    10921 gctgggaacg gtggttcaag ctgcgggctt ggtagatgat acggtcaatg tggcaaatga
    10981 atacagccga tacccacttg aaaactatca actggatttt tatgtggata atagctgggg
    11041 ctggcttccg tggaactggt cggacgggat tggaaaacag gtcatgtatg gactatatgc
    11101 cattaccaat tttatttgga caatcagttt gtatgtttcc aatgcgacag gttacttagt
    11161 acaggaagcc tattccttag acttcatttc cgctacagca gattccattg gtaagaatat
    11221 gcagacctta gctggtgtga gtgcaaacgg attttcaaca gagggtttct atgttggatt
    11281 cctcttactc ttgattttgg ttcttggggt ttatgttgcc tatacgggac tgataaagag
    11341 agaaaccaca aaggcaattc atgccattat gaattttgtg ctggtgttta tcctatcggc
    11401 ttcctttatt gcctacgctc ccgactacat taaaaaaatc aatgactttt catcagacat
    11461 cagtaatgcc agtttatcac ttggcacgaa gattgtcatg ccccattccg atagtcaagg
    11521 caaggacagc gtggacttaa tcagagatag cctgttttcc atacaggttc agcaaccgtg
    11581 gctactgctt caatacaaca gttcagacat tgaaagtatc ggtattgacc gtgtggaaag
    11641 cctgctctcc accagcccag attccaacaa tggcgaagac agagaaaaaa ttgttgcgga
    11701 agaaattgaa gacagaagca ataccaatct aaccattaca aagaccatta accgtttagg
    11761 tacagtcttc ttcctatttg tcttcaatat tgggatttcc atatttgtat tcctattaac
    11821 aggaatcatg attttctcgc aggtactttt tatcatctat gctatgtttc tgcctgtgag
    11881 ctttatttta agcatgattc catcatttga tggtatgtca aaacgagcca taacaaagct
    11941 ctttaatacc attttgacac gagctggaat cacattgatt attacgacag catttagtat
    12001 ttcaaccatg ctctatacct tatcggctgg ttatccgttc tttttgattg cttttctaca
    12061 gattgtgacc tttgcaggaa tctacttcaa gctgggcgat ttaatgagta tgttttctct
    12121 acagagtaac gattctcaaa gtgtgggaag tcgtgtgatg agaaaacctc gtatgcttat
    12181 gcacgctcac atgcaccgtc tacagcggaa acttggacgt tccatgacta ctctaggggc
    12241 tgggtctgcc attgttacag gtaaaaaagg acagtcgggt tcggggagtt ctgcaaggac
    12301 acaagcagat cactcccgac cagacggaaa ggaaaaatca acacttggaa aacgtatcgg
    12361 tcaaaccatc ggtacagtag ctgataccaa agacagaatg gtagacactg ctagtggttt
    12421 gaaagaacag gttaaagatt tgccgaccaa tgcaagatat gcagtatatc aaggaaaatc
    12481 caaagtaaaa gagaatgtcc gtgatttaac cagtagtatt tctcaaacca aagcggaaag
    12541 agccagtgga cgcaaggaac agcaggaaca aaggcgaaaa accattgcga agcgtcgctc
    12601 tgaaatggaa caggtcaaac agaaaaaaca gcctgcttct tctgttcatg aaagaccgac
    12661 tacaagacaa gaacaatatc atgatgaaca gacctcaaaa cagtctaata ttcagacttc
    12721 atataaggaa tctcaacaag ccaaacaaga gcgtccagca gttaagtccg atttttcaag
    12781 tccaaaagtg gaacgccaag gcaataccgt tcaagaaaaa accgttcaaa agccagcaac
    12841 ttcaaccact acagcagata gaacttcaca acgtccaatc acaaaagaac gtccgtctac
    12901 tgttcaaaga gtaccactac aaaatacaag aagtagacca ccaatcaaaa ccgccaccat
    12961 taagaaagtc ggtaagaaac catgaagttg aaaactttag tgattggtgg ttctggatta
    13021 ttcttgatgg tcttctcact gcttctgttt gttgccattt tattttcaga tgaacaggac
    13081 agcggaattt ccaatattca ttatggaggt gtgaatgttt ccgcagaagt gctggctcat
    13141 aagcctatgg tagaaaaata tgccaaagaa tatggcgttg aagaatatgt caacatactt
    13201 cttgcgatta tacaggtgga atcgggcggt actgcggaag atgttatgca gtcctcggaa
    13261 tccctcggtc ttccacctaa ttcattgagt acagaagaat ccattaagca aggtgtgaag
    13321 tatttcagtg aattattagc cagtagcgaa aggctcagtg tagatttaga atcggttatc
    13381 cagtcctaca attatggtgg tggtttctta gggtatgtgg ctaatcgtgg aaataaatat
    13441 acctttgaac tggctcaaag tttctcaaaa gagtattcag gtggcgaaaa agtgtcttac
    13501 cccaatccca tagccatacc tatcaatggg ggctggcgat acaactatgg caatatgttt
    13561 tatgtgcaac tggtaacgca gtatcttgtc acaacagagt ttgatgatga tacggtacaa
    13621 gccatcatgg acgaagcact gaaatatgag ggctggcgat acgtttacgg tggagcttcc
    13681 ccgactactt cttttgattg tagcggactg acacaatgga cgtatggaaa agctggaatt
    13741 aacttaccac gaaccgcaca acagcaatat gatgtgaccc agcatatccc actatcggaa
    13801 gcacaagctg gcgatttggt tttctttcat tctacctata acgctggctc ttatattact
    13861 catgttggga tataccttgg caataaccgt atgtttcatg caggcgaccc aatcggttat
    13921 gccgacttaa caagccccta ctggcaacag catttagtgg gagcaggacg aatcaaacaa
    13981 tgagaaagga agatttaatg atgaaattta gaaaaaatca gaataaagaa aaacagatac
    14041 caaaggaaaa gaaacctcgt gtctataagg tcaatcctca taaaaaggtt gtgattgcct
    14101 tgtgggtact tttagggctt agtttcagct ttgcgatatt caagcacttt acagctatag
    14161 atactcatac tattcacgaa acaactatca tagaaaagga atacgttgat actcatcatg
    14221 tagaaaattt tgtagagaac tttgcgaaag tctactattc atgggagcaa tccgataagt
    14281 ccattgataa tcgaatggaa agtctaaaag gctatctgac agatgaactt caagctctca
    14341 atgttgatac agtacgcaaa gatattcctg tatcgtcttc tgtaagagga tttcagatat
    14401 ggacggtaga gccaactggc gacaatgagt ttaatgtaac ctacagtgta gaccagctca
    14461 ttacagaggg agaaaataca aagaccgtcc actctgctta tatagtgagt gtctatgtag
    14521 atggttctgg aaatatggta ctggttaaga atccgaccat taccaacata cctaagaaat
    14581 caagttataa accaaaagcc attgaaagtg aggggacggt tgattccatt acaaccaatg
    14641 aaatcaatga gtttttaacg acgttcttca agctctatcc tacagcgaca gccagtgaac
    14701 tttcctacta tgtgaatgac gggatattaa aaccaatcgg aaaagagtac atctttcaag
    14761 aactggtaaa tcctattcac aatcgtaagg ataatcaagt cacggtatcg ctgacagtgg
    14821 agtatatcga ccagcagacc aaagcaacgc aggtatctca atttgatttg gtacttgaaa
    14881 agaacgggag taattggaag attatagaat aacaaatatt ggtacattat tacagctatt
    14941 ttgtaatcac gtactctctt tgataaaaaa ttggagattc ctttacaaat atgctcttac
    15001 gtgctattat ttaagtatct atttaaaagg agttaataaa tatgcggcaa ggtattctta
    15061 aataaactgt caatttgata gtgcgaacaa ataattggat gtcctttttt aggagggctt
    15121 agttttttgt acccagttta agaatacctt tatcatgtga ttctaaagta tccggagaat
    15181 atctgtatgc tttgtatgcc tatggttatg cataaaaatc ccagtgataa gagtatttat
    15241 cactgggatt tttatgccct tttgggcttt tgaatggagg aaaatcacat gaaaattatt
    15301 aatattggag ttttagctca tgttgatgcg ggaaaaacta ccttaacaga aagcttatta
    15361 tataacagtg gagcgattac agaattagga agcgtggaca aaggtacaac gaggacggat
    15421 aatacgcttt tagaacgtca gagaggaatt acaattcaga caggaataac ctcttttcag
    15481 tgggaaaata cgaaggtgaa catcatagac acgccaggac atatggattt cttagcagaa
    15541 gtatatcgtt cattatcagt tttagatggg gcaattctac tgatttctgc aaaagatggc
    15601 gtacaagcac aaactcgtat attatttcat gcacttagga aaatggggat tcccacaatc
    15661 ttttttatca ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt
    15721 aaagagaaac tttctgccga aattgtaatc aaacagaagg tagaactgta tcctaatatg
    15781 tgtgtgacga actttaccga atctgaacaa tgggatacgg taatagaggg aaacgatgac
    15841 cttttagaga aatatatgtc cggtaaatca ttagaagcat tggaactcga acaagaggaa
    15901 agcataagat ttcataattg ttccctgttc cctgtttatc acggaagtgc aaaaaacaat
    15961 atagggattg ataaccttat agaagtgatt acgaataaat tttattcatc aacacatcga
    16021 ggtcagtctg aactttgcgg aaaagttttc aaaattgagt attcggaaaa aagacagcgt
    16081 cttgcatata tacgtcttta tagtggcgta ctgcatttgc gagattcggt tagaatatcg
    16141 gaaaaggaaa aaataaaaat tacagaaatg tatacttcaa taaatggtga attatgtaaa
    16201 atcgataagg cttattccgg ggaaattgtt attttgcaga atgagttttt gaagttaaat
    16261 agtgttcttg gagatacaaa gctattgcca cagagagaga gaattgaaaa tcccctccct
    16321 ctgctgcaaa caactgttga accgagcaaa cctcaacaaa gggaaatgtt acttgatgca
    16381 cttttagaaa tctccgacag tgacccgctt ctgcgatatt atgtggattc tgcgacacat
    16441 gaaatcatac tttctttctt agggaaagta caaatggaag tgacttgtgc tctgctgcaa
    16501 gaaaagtatc atgtggagat agaaataaaa gagcctacag tcatttatat ggaaagaccg
    16561 ttaaaaaaag cagagtatac cattcacatc gaagttccac cgaatccttt ctgggcttcc
    16621 attggtttat ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga gagctcggtt
    16681 tctcttggat acttaaatca atcatttcaa aatgcagtta tggaagggat acgctatggc
    16741 tgtgaacaag gattgtatgg ttggaatgtg acggactgta aaatctgttt taagtatggc
    16801 ttatactata gccctgttag taccccagca gattttcgga tgcttgctcc tattgtattg
    16861 gaacaagtct taaaaaaagc tggaacagaa ttgttagagc catatcttag ttttaaaatt
    16921 tatgcgccac aggaatatct ttcacgagca tacaccgatg ctcctaaata ttgtgcgaac
    16981 atcgtagaca ctcaattgaa aaataatgag gtcattctta gtggagaaat ccctgctcgg
    17041 tgtattcaag aatatcgtag tgatttaact ttctttacaa atggacgtag tgtttgttta
    17101 acagagttaa aagggtacca tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca
    17161 aatagtcgga tagataaagt acgatatatg ttcaataaaa taacttagtg tattttatgt
    17221 tgttatataa atatggtttc ttgttaaata agatgaaata ttttttaata aagatttgaa
    17281 ttaaagtgta aaggaggaga tagttattat aaactacaag tggatattgt gtgctgagag
    17341 ctttcttcta tactaataga cgaaagggtg tgaaaatgat ttttaaatga tactgtggaa
    17401 cggaacagta gccctagtat tgactactgt cgtttctatt catattggct attctaggac
    17461 tgagatgaaa aaatctataa atgctcagaa taaaattgaa cccgcaaatc tccccaaaac
    17521 aatggtgagt catgtacttg tattattccg aaaaaataca cctctggtgc agtgagacaa
    17581 attggtgtat cttatagtgg cttcgtagat gaaagctata ctctactatc actctttgat
    17641 gatgtagaac aaattgaaaa agataataga cttcagacag ctattgatgt tgtcagagaa
    17701 cagtttggtt ttttagccat acaaaaagga accgtcctaa ctgaaggttc cagaaatatt
    17761 gaacgcagta aacttatcgg tggtcattcc gcgggtggat tggagggatt aaaatgaaac
    17821 aagaaaaaaa tacagtacaa ttttcagaaa tccgtagcaa aggatgtaat gatattgaaa
    17881 tgcttgaaag atttttacat ggaatcgttg aaacagcaac ttcaaaactt cgtcagagaa
    17941 aactcaaaac aactgaaata tcgatacgac tagtacatgc taaatctgaa aaccgattac
    18001 cattggaatt tacatttagc attaagccaa caagctcatc tgtgataatc tatactgagg
    18061 taatcaatcg ctttaaagaa tgttacacag gtgggggaat tcaaggtttt acgattcaat
    18121 ttgataaaaa tacccttgcc tctgcataga aaggatttga tatgattgac cgttcatatt
    18181 taccatttca atcagcaaga gagtaccagg atacaaagat gcaaaaatgg atgggctttt
    18241 tcctatctga acatgcatca gcactctctg atgatacaaa caaagtaacg tacatgtctg
    18301 acttatcact agagaagaaa ttattactcc tcagtcaagt atacgccggg cagctacgca
    18361 cacgcattca agtgattgaa aaaaacaagc gtgtttccta cactggaaca ataccaagtc
    18421 tgaccaaaga tttcattttg ataaaaacta caacaggtca catcaatttg aaattaaaag
    18481 acattattag tattgaactt gtcgaggagg tgctctatga atcagcttga gtttcagcgt
    18541 aatcacctac aaatggacta ttatagcgag agctaccaag attttgaacg tgacttctac
    18601 cgctactcta acatgaatat tccattgacc ttcctaactg atgatatcct aaaaacaatg
    18661 gcgacttcac gtaagaatta ctttgtcctc aataaggaaa agtccagaga taaccgcgat
    18721 cacttcttca tatttgaagt aagtaccgta gatgagaatc cgctaatcta tcattataca
    18781 tataagaaaa ctacaatata tttagcagaa aaataggagc agttcaattg actgttccta
    18841 tttttaatat tcataaaatc taaagtcttt atactcttta acaatggagt cgccaaccag
    18901 aacagactat actgaccagc gactacctta aatttaatgt ttcagattta ttttcttatc
    18961 tctaatttca taaactacat ctgctacatt ttcgagtaat cgtttatcgt gggtgataaa
    19021 cacgatagtt ccggtgtact ccttcattag tatttccaaa gcctctaaac ttggtatgtc
    19081 aaggaagtta ctgggttcat ccattattag gatgttatat ctacccatga gcattttagc
    19141 aagcaacaat tttataattt ctccaccgct taaaacagat aaactttttc caatatcgtt
    19201 ctgtttgaac cccatagatg ctagcactga acgaatttct gatatattgt agtcacaatc
    19261 cttctgcata aactccataa cattctgatt actgttgtac ttgtaaccat tctgtgcaaa
    19321 gtaacctatt tttgccttag gcgaaataga aattccttct tcatggttta agatcatttg
    19381 gattaaagtt gtttttccga ttccattacc accagttaac gccacttttg ctcctaacgg
    19441 aatttgaaaa gatgcatttt caaacagagc cttatcccca aatactttat taatttctgc
    19501 accgactata gggtatggat tatggagctc caatgcttta ctttgcctga aacgaattct
    19561 gcgaatgcct tccggagctt ctacttttcc taaggccgca atcctgtgct ctagggtttt
    19621 agcagcatta tacatctttt tttccttact tcctattgat ttttgatgag ctaaacgccc
    19681 tccgtcttca gtactttttt tctttgaaga accttttgcc ttctgttcta ttttacgagc
    19741 ctgttttcgc ttttcctccg cagccctttc caatcgggca cgttccgcaa taaattgttc
    19801 gtattctgca gcttggctct tacgttcttc ctctttctga cgaagataat cagaatagtt
    19861 tccccaatac tcagtgattt tgccatcttt cagttcccat attttatcta ctatttcatc
    19921 aagaaaatag cggtcatggc taataactaa cagtgcacct gtaaaatatt ttagctgtcc
    19981 tattagaaaa tcaattcctt cacggtctaa atggctcgta ggttcatccg ctaaaatacc
    20041 atgaacctgt gccgataagg cctgtgctat tttaagcctt gtttcttcac caccgctcat
    20101 agtctgtata tttaattgct caacacctag cttgcctaca agtgcaaaat ctttttcctc
    20161 ctgcagagtt acttcgtcca actggggaat ataggcaagt tcacccagac gattcatttt
    20221 acatcctggg ggagttaatt ctcctaaaag taccctgagt aaagtgcttt ttccagcacc
    20281 atttgctcct actaaaccaa tacggtcata atcatatact tctaattcat ttatatctaa
    20341 aacatcgcgt cctttgaatt ccacacgaat gtcttttgct tttaatatta attccataac
    20401 atttcctcct gtctataatc gcatgctttc atttgcttgt atgcagggaa aaccctgcga
    20461 ttttagcagg aagagttaca tgaaaataag atacataaat attcctccaa tattgtttat
    20521 tttaaatcta attttctaac ctcagttatc atttggcaaa ctatagcaat gccaataatt
    20581 aaaatacctg atagtaaaaa ccaatgattt acaccgattt tatcagcaaa gaatccagaa
    20641 agaattaacc caattggcat agcaagtgac atgatacttc cgatcaaaga aaatacacgt
    20701 cctaaatatt caggcttaat tttctcctga aaaagagctg tttgcacacc gctataaaat
    20761 ggcaccgaaa gccccattat tgcacagcaa actacgaata ttacaaatcc atttggagga
    20821 agtattcccg aaacggctaa actggtcccc attataaaaa atgaacttgt tattagtaat
    20881 acatgctttt cgaagccccc taatcttcct aataataagc ctcctgctag catcccaaat
    20941 gcaaaggaaa tttccgtaat agaaatatgc acaggcgttc cattaaagtg ttccatgctt
    21001 attaaaggaa atagtgcatt gattggcata taaacaaaag tatatagtgt tcctaagagt
    21061 aataaggcaa acaatccttt gttttgtctc agaaccacaa ctccttcttt catctccctt
    21121 atgaaatttg gttctaaact ttgcacttga ttacccagct taggtatacg tacaattgct
    21181 accgtaatag atgcaatcac agcacccaat acgtcgatgg caataatagc atttaaatcc
    21241 caaacggagt ataagagtgc tgcaactgcc ggactaacaa tatagcttat agactgcaaa
    21301 gactgactat agcctgcgca tttcgttagc tgttcttctg gtactaaaag tggtgtaacc
    21361 gcattgagtg ctggggtatg aaaagctgtt ccaatgctac ggataaacaa tactatcata
    21421 atcatccaga caggtagctc catacagaat gcaacaatag caagcactgc accagctgct
    21481 gcgataatta aatcggcacc aatcattatc ttcttcctat catgacgatc cactagcaca
    21541 ccaatggcag gtcccaaaat cgcatagggt aaaaaaccta ctaatgaagc catagacaag
    21601 accatcgcag atcctgtttt ttctgtaagg taaaaaataa tcgccatttg caggatggca
    21661 ctagtgatta atgatactgc ttgccctgcc catattgcat aaaattttcg tttccaattg
    21721 ttgtattttt ccatttatat tatctcctgc atattatttt gcttgaattt ctattttgaa
    21781 tagcattcta ggcaataaaa aatgcaggcc aaaccccaca atgtggcttt tggtctgcat
    21841 acatacaatt tggaaacatt catattaaag acatagttaa ataaaggtat agttaaataa
    21901 ccaatatcct caccgtaact aatgaatgct caatatcgta taaataagca caacaaaaaa
    21961 gcctatcatc gggtatagat tctgcttttt ttattgccag cttatcttaa acgcattgag
    22021 gctgtcatag tttcggttcc tcctacatct ttgtttatat caatttatag tataacacaa
    22081 caagatgata tgttcaatat aaaagttatg gaatgagact catacttcca attcgatgcc
    22141 agatttaaag gatatgacga agttttcttc atagactgta acgctctgga ttatcttcct
    22201 tagtagcaag cgattagctt tcacaaaatc ttctgtttgt agttttaaaa attcatcagg
    22261 attttctaac tcaacctcaa aatatttcat tttacattcc ctcatttcat ttattgataa
    22321 attgagtttg caaaaaagag tggacaattt ttgtctactc ttaaccttta aaatagtttt
    22381 ttttaatcga tttgaagttg cctaaattat tacttattcg gtaaaatgaa gtattgcttt
    22441 caacagattt ccttcaacta cacttcactt gattcaaaca aggtgggtac atttctattc
    22501 ccacaaactc cttgtcaatg gaaacaaaca cgtacccaca gggtaaatgg aaatagaaac
    22561 tgataatttc tagctatcac ttctactcat tccaaaaatt ttctcactct gatacttacc
    22621 caccataaag caaaaagcct tgcaatcaag gctttcatta tccctttcgt tcaaaggttt
    22681 ctaagctttt acgagcagag cgacacactc agcggttcgc tatctccgtt ctgtctgcgt
    22741 gctagcactt gtcaatcacg gacagctatc gcatgggcgg aagtaaatgc taatcttcgt
    22801 cgttttactc cttgactagc aaacttaccg cctcaacatg tcctgtatgt ggaaataaaa
    22861 cacgattaaa gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat
    22921 gcagacaaga aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag
    22981 acgcaaagac gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc
    23041 cgttatgtat ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg
    23101 ctaaagattc cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct
    23161 gtaatcgtcg ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa
    23221 catgaagtaa cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt
    23281 caatattagt taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta
    23341 aacctcgttt caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat
    23401 cttctttttt acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa
    23461 acaggtatag aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact
    23521 aaacgccaat aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat
    23581 attacttcca aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa
    23641 taaatttcct tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac
    23701 gtccaccaat gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga
    23761 aataaatctg tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc
    23821 agtaatgcaa caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca
    23881 aggctcactt cctttcctag acagaaaggg ggtgagaaac atgaaaccat cttcttttca
    23941 gaccacaata gaaaatcagt ttgactatat ctgtaaacgt gctatggaag acgagcgaaa
    24001 gaattatatg ctttatcttt caaggattgc aaagcgtgag gtgtcctttt cggatgttgg
    24061 cgattatctt gttagccagt ttgcgacaac agataactat tcaactgact ttcagatttt
    24121 tacactcaat gggttatcag taggcgttga aaatgatttg ttgagtgaag cattacgtga
    24181 gttgccagac aagaaacgtg aaattctact gctgttttac tttatggaca tgagcgattc
    24241 agaaattgca gacctgttga aattgaaccg ttctactgtc tatcggcata gaaccagtgg
    24301 actagcctta attaaaaagt ttatggagga atttgaagaa tgaaaacaca atatcctatg
    24361 attccctttc ctctcattgt aaaggcaaca gatggcgata ccgaagcgat taaccagatt
    24421 ctacatcatt acagagggta cataacgaag cgttccctac gacttatgaa agatgaatat
    24481 ggcaatcaaa gtatggtcgt tgatgaagtc ttacgtggaa gaatggaaac cagactgatt
    24541 acaaagattt tgtcatttga aattaagtaa tatcctctct cctttcgtgg aagcgtgcta
    24601 aaccattcca cgcttcccga acagggaggt ttgttattcc accaaagcat attgagcttt
    24661 caatgtgttt tgataggcta acgagccatt gttctttgaa aactgaataa aagtaatcga
    24721 atacgtttcg ataagaaaag agccaacgga actaaccgcc atgacctatc ttataaagat
    24781 agcgagcgat tcatgttagt gatccgagaa gcaatcttta gcaggattgc ctgcaacgac
    24841 attcttatcg tgataatgat actcccatac agtcaatagt ccgagcgtga taaaaccgtc
    24901 gcaggcaatg agtatggcta catgagaacc atgcaggggt ggaactcccg tgagctttgc
    24961 taaagctgtt cgattgctgg taaaacaact tttatgaaat ccaaataagt gatttggaaa
    25021 ggaggatttt atgaagcaga ctgacattcc tatttgggaa cgttataccc taaccattga
    25081 agaagcgtca aaatattttc gtattggcga aaacaagcta cgacgcttgg cagaggaaaa
    25141 taaaaatgca aattggctga ttatgaatgg caatcgtatt cagattaaac gaaaacaatt
    25201 tgaaaaaatt atagatacat tggacgcaat ctagcgtcgc caaagggtct tgtatatgat
    25261 aaaatagtat taagtcgtat caaggctctt tccataaagg aaaggagcaa atgccatgtc
    25321 agaaaaaaga cgtgacaata aaggtcgaat cttaaagact ggagagagcc aacgaaaaga
    25381 cggaagatac ttatacaaat atatagattc atttggagaa ccgcaatttg tttactcgtg
    25441 gaaacttgtg gctacagacc gagtaccagc aggaaagcgt gattgtatct cacttagaga
    25501 gaaaatcgca gagttacaga aagacattca tgatggtatt gatgttgtag gaaagaaaat
    25561 gacactctgc cagctttacg caaaacagaa cgctcaaaga ccaaaggtta gaaaaaacac
    25621 tgaaactgga cgcaaatatc ttatggatat tttgaagaaa gacaagttag gtgtaagaag
    25681 tattgacagt attaagccat cagacgctaa agaatgggct attagaatga gtgaaaatgg
    25741 ttatgcttat caaaccatca ataactacaa acgttcttta aaggcttcat tctatattgc
    25801 tatacaagat gattgtgttc ggaagaatcc atttgacttt caactgaaag cagttcttga
    25861 tgatgatact gtccctaaga ccgtactaac agaagaacag gaagaaaaac tgttagcctt
    25921 tgcaaaagct gataaaacct acagcaaaaa ttatgatgaa attctgatac tcttaaaaac
    25981 aggtcttcgt atttcagagt ttggtggttt gacacttcca gatttagatt ttgagaatcg
    26041 tcttgtcaat atagaccatc agctattgag agatactgaa attgggtact acattgaaac
    26101 accaaagacc aaaagtggcg aacgtcaagt tcctatggtt gaagaagcct atcaagcatt
    26161 taagcgagtg ttagcgaatc gaaagaatga taagcgtgtt gagattgatg gatatagtga
    26221 tttcctcttt cttaatagaa agaactatcc aaaagtggca agtgattaca acggcatgat
    26281 gaaaggtctt gttaagaaat acaataagta taacgaggat aaattgccac acatcactcc
    26341 acatagtttg cgacatacat tctgtaccaa ctatgcaaat gcaggaatga atccaaaggc
    26401 attacagtac attatgggac atgctaatat agccatgacg ctgaactatt acgcacatgc
    26461 aacattcgat tctgcaatgg cagaaatgaa acgcttgaat aaagagaagc aacaggagcg
    26521 tcttgttgct tagtagtaca aatgaattta ctacttattt accacttctg acagctaaga
    26581 catgaggaaa tatgcaaaga aacgtgaagt atcttcctac agtaaaaata ctcgaaagca
    26641 catagaataa ggctttacga gcatttaaga aaatataaaa agataattag aaatttatac
    26701 tttgtttatt ttaaagaagt gattctaatg tacaagggtg taatttgtta gaatttcaaa
    26761 aatttagagc atttttggct tagtaatatg tgttgaaggc tcaaaatcta tggtaaaaaa
    26821 gtagctttga aaacgtatta cctccaaaga tttagtttaa taatgaatta acataaaaag
    26881 aaatgattga agttctggaa agatgttgtt tcagtattga aatcattgag aaaaagtggg
    26941 aaaaatttgc gattttcaca gagaaaggaa gaaaaagtat agaaataagt caaatggaga
    27001 tgtttgataa aatgctttca tagctaacca actgttgaaa attatgagtt acaaaatcta
    27061 aagtatttaa gattagttta taaaattagg tctctgcctg tagaaaggaa cgtgcagtta
    27121 gaaaggagga aaaaatagga tactataatt cagactgaga tttctaatta aaatatgtag
    27181 ttgcatcatc ccttgccgtt ttggcattta gtttggctct tcctgttttg gtagcttctc
    27241 accgtggtgg cgagtggacc tatggaggct atcatgatcc aaataattgg ggagcaaatt
    27301 acatttgatg cgggatggta aagaataatt gaagaagatt cttttatcaa tagtttagta
    27361 agcttgttag tttatagagg tcaggggtat ttctgccctc tgttttagaa taaggaatgc
    27421 actatgaaaa aattatttat actcatatcc aacctgcttg ctagtttgtt ctttgtttgg
    27481 gtatttacca tttggactga tacatatgtc tcctattatt accccaacgt tgttgtacgt
    27541 gattcttccc ctgaaacaac ttttcagcat gttgctacac gcttggaaaa actagcagaa
    27601 gaaacggata gctttattgc gattcaacac caagatccta actcagaagg tactccagtg
    27661 ttttcttata cgacctttgg gaacggaaag ttgcctgatg ggcttcagga aaaaaatcta
    27721 gaagatgctc aaagtagtag tgttgaaaca aactattttg tattcgatgg aaacctagat
    27781 attcacttgc taagggagga gctaagtcaa cttggcttga ctaacatgca cctgacaatt
    27841 ccatctaaac tatctacgtt aatggctatc tttagtaacg gatttcagtt aatcagtcta
    27901 ttgattttca tcttaacctt tggagcacta actttaatta gtcaaattag ccaattacgg
    27961 tcgtcgggca ttcgcctcat ttcaggagag aaacggtggt tcatttttct tagaccagtt
    28021 ggtgaggatt tgaaagggat agctgttggt ttcagtttag ctggcgtgct caccattctt
    28081 atgcaaaaaa ttctgtcgct tccaacgcaa tccctaatga ccataggagc aggcttgctc
    28141 agttataatc tcatcttgtt gtcgatctcc ctgtttttcg ctcaactctt cgcagttggg
    28201 ataaaaaaga tacacctgat gcagattata aaagggcaag tgcctgtcag aggaatcatt
    28261 agtttgattt taatcggtca actattagcg attattattg taacgctggg aatagggagt
    28321 agcttaaagt attcccaagc ctggcaacag catcggattg gacaagaggc ttggagccag
    28381 gaaaggcaac tgattaccct atcaatcagc cgtgaaggaa cgagtcctgg ttttgatgaa
    28441 caagctcaaa ggaaactcag aacttggtat caattgatgg atctggctgt ttcagaacaa
    28501 aaggctttct tatctagaca ccagttaatt gaccgtactt tgcaaaatgg tatggcttcc
    28561 tccaaaaact ttataacctc tacagaatgg tacgattaca gcccgaatgg caatgtcctt
    28621 atcgtcacac cgcaatactt ggagcgtcaa aacattcctg tagatacaac tattgagcaa
    28681 aagatgaatc accttgatgt aggggagttt gtcttattgc tgcctgaaca cctccgttca
    28741 gaggaagatc attataaatc tgtttttgaa gacgacttaa ccagtcgcat gtctagtcaa
    28801 gatgaacgac agcaaatgac tgctacggta ggttatttag aatcaggtca ggatcgtttt
    28861 gtgtataata cgacccctat ttcttaccag cagtttttga aagatccaat catcattgtt
    28921 ataacacccc aatcaactgg tccacagtcc attttgtttt ggatagacgc agtacagaac
    28981 tacgttctct ttaatcaatt gtctgatgcc caggagctta tccagagaca aggcattgaa
    29041 aattgggtct cagaaatgca aacaggttac cacaactaca tcacattatt ggataatatc
    29101 cagagggaac gttgggtaat gctagcagga gctgtgcttg ggattgcaac ttcaatcttg
    29161 ttgtttaaca ctatgaatag gctctacttt gaagaattta gacgtgccat ttttatcaaa
    29221 cgcattgcag gtctcaggtt cttagaaatc catcgcactt atctctttgc tcaactgggt
    29281 gtgtttttac tgggatttgt tgcgagtgta tttcttcagg tagagatagg agttgctttc
    29341 ttagtcttgt tactctttac tggtctatct cttttacagt tacatgtcca aatgcagaaa
    29401 gaaaacaaga tgtccattct tgttttgaag ggaggttaat atgattgaac ttaaacaggt
    29461 gagtaaatct tttggagaac gagagttatt ttcgaatctt tcaatgacat ttgaggctgg
    29521 aaaagtctat gccttaattg gttcaagtgg tagcggaaaa acaaccttga tgaacatgat
    29581 tgggaaatta gaaccttatg atgggacgat tttttaccga ggtaaagact tggccaatta
    29641 taaatcgagt gattttttcc gtcacgaatt gggttacctc ttccagaact ttggcttaat
    29701 tgaaaaccaa agtattgaag aaaaccttaa gctaggtctc actggtctgg tcaaaagttg
    29761 agtcggtcgg aacagcggtt gaggcagaag caggctttag aacaggtcgg cctggcttat
    29821 cttgacctag ataagcgcat ctttgagtta tcgggcggag aatcgcaacg ggttgccttg
    29881 gcaaaagtta tcttaaagaa tccacccttt attctggcag atgagccaac agcttcaata
    29941 gacccagcaa cctctcagtt gattatggag attttgctat ctcttcgaga tgataatagg
    30001 ctaatcatta tcgcaacaca taatccggca atttgggaga tggctgatga agtgttcacg
    30061 atggatcatc tgaaataaaa atccttgttt ttaattgcac gatgagttac tgaaatatta
    30121 tcatgaatca agaattggag ttaatttaga attgtacttt attaatattg aggtaacttt
    30181 ttcttgataa aggaagaaat aatggagagg aagttagaat gaaaaaattc gacaattata
    30241 ttattgagaa gccttgcgat tctaattcag ataaactgca aaaaatctta ataattgaaa
    30301 gtttggtaga tgatattttg caattttctc tcagaatcaa taatagtgta ggagagattt
    30361 tcctcctaca accgttttaa aagaaaacta tctttattcc atgttatttt gaggaagata
    30421 ttgtgaaagt caaagatgat gataaagttg agtggaattt gttagaattt caaaaattta
    30481 gagcattttt ggcttagtaa tctgtgttga aggctcaaaa cctatg
//
LOCUS       DAVAAL010000012        29710 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVAAL010000012 DAVAAL010000000
VERSION     DAVAAL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29710
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.012"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(68..1099)
                     /locus_tag="VQM60_000410"
     CDS             complement(68..1099)
                     /locus_tag="VQM60_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET1080307.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(1354..2037)
                     /locus_tag="VQM60_000411"
     CDS             complement(1354..2037)
                     /locus_tag="VQM60_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET1080308.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(2049..2993)
                     /locus_tag="VQM60_000412"
     CDS             complement(2049..2993)
                     /locus_tag="VQM60_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET1080309.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            complement(3154..4319)
                     /locus_tag="VQM60_000413"
                     /pseudo
     CDS             complement(3154..4319)
                     /locus_tag="VQM60_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(4404..5918)
                     /locus_tag="VQM60_000414"
     CDS             complement(4404..5918)
                     /locus_tag="VQM60_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HET1080310.1"
                     /translation="MRKYDIPKLLLSGENQGVEFKEAKNSFPKDGMKTICSFANTNNG
                     LLILGVSENTKNKDFYISGVNDPDKVLDELYSLLNNPNKINRNVINEESVEVVNIKGK
                     EIVIIPVNKVDYKDKPIYLNDNIASTYFRQGTGDFRCSQEQINSMLRDSAKESFDSTL
                     IQDFSILDLDTETIKLYREKFDNINAEHPFSKLDNEQFLIKINALRRDRKDDKIKPTV
                     AGLLIFGTHNSIKEFIPHYNVEYVLKEFSENNRFKDRVIYDGTWGEDNLFNFFYLVIE
                     KLYLTLNDNSNIQENSMNRIGISKLRIAIREAFINSLIHSDYKSEKGIMIIRYPDRYI
                     FTNGGTLRIDIKDFFSGAHSDPRNYLIQEIFRFLNLCEKAGTGIPKIMEAVKESHLKY
                     PNLRTELDSVEFTLWDTSLIDNLDIDNEYEKKILELIIEHRFVTREALEEKLKIHKNT
                     ILKYLKKLVEKQAIDKFKSGRQYVYFITQNQDPEFQKYNHINAMYSLLEEIKRK"
     gene            complement(6079..6792)
                     /locus_tag="VQM60_000415"
     CDS             complement(6079..6792)
                     /locus_tag="VQM60_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET1080311.1"
                     /translation="MAKNSPQDLKNREYFSNNLNRVMKEKGIRQIDLHNALEIPKSTI
                     TGYVKGRSMPTSGNLQKLADYLGVKKSDLDPRFSDKDVYKEFLYMLDKQLDEEKYLVK
                     NVSKDKIFQKVKEREKRDKEIIELAKSKNFPISPEEFLTTITESLNEYNPKDNYEVLN
                     ALALKTSNILFWLDIVFQKTSSEFYIVFEDKEFNQEYYNKLYKKFENLFEEIKSLALQ
                     EAKKDKENLDDFALLWGID"
     gene            6945..7175
                     /locus_tag="VQM60_000416"
     CDS             6945..7175
                     /locus_tag="VQM60_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1080312.1"
                     /translation="MAQWTLRACRVNAGFTLRQVAKKVNKNFQTISKYEKDSTLIPFE
                     LLKELSELYQVKLDDIFLGDSTKKIELNQKEI"
     gene            7191..7817
                     /locus_tag="VQM60_000417"
     CDS             7191..7817
                     /locus_tag="VQM60_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HET1080313.1"
                     /translation="MELVYMDGKKEPYTLSSIVAECAEVKHRHLKILLNKHREDFESF
                     GKVQFKISPSESGQNVRDYILNEQQATLLITYLRNTEPVKEFKKNLVKAFFEMREELS
                     KFRMQRALEKPKRKTLHDSIETWEAKPKHAHSTMNNLLLKAVTDMNAKQLREERGGYN
                     GIDSLTSDELEQYQAFEDMVIAMIGLNMSYQEIKAMAFRNKKTRQRSA"
     gene            8189..8641
                     /locus_tag="VQM60_000418"
     CDS             8189..8641
                     /locus_tag="VQM60_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080314.1"
                     /translation="MTETTYENLTRHIDRISAEMREIGEKNGLSRLSLLANQTKFIKE
                     NLHRLLWIELPELNDSHKIEAVSKSTTGMFFHAGIFEMDAMRQAFFKRQAMHFFDNEA
                     EQQAYIEHAEKEYLEATITLKDILFNSKNGTQRVNKSCLIEKFEEAMQ"
     gene            8638..8838
                     /locus_tag="VQM60_000419"
     CDS             8638..8838
                     /locus_tag="VQM60_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080315.1"
                     /translation="MTLNLDNMTQAEFDEVMAEIKEKRPNLFQFIADFVDRKVSTEEV
                     DEYLKMKHEKQVEFIKNYQARA"
     gene            8840..9055
                     /locus_tag="VQM60_000420"
     CDS             8840..9055
                     /locus_tag="VQM60_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080316.1"
                     /translation="MNELDLTNTQSVIFMVVLIGLLLYLNHRDRKKSAQFERENQQRI
                     ETPSEDLSPDYGRYIQLAGVRVYGGTE"
     gene            9052..9393
                     /locus_tag="VQM60_000421"
     CDS             9052..9393
                     /locus_tag="VQM60_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET1080317.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGAEISLITKLAERTVQDIISR
                     LITRHSVPIVGVRHGTFRGYFIPANKEELLDGAKAFYNQVQEEQKRLTVLLNADLESY
                     KETLKEVGGYV"
     gene            9386..9658
                     /locus_tag="VQM60_000422"
     CDS             9386..9658
                     /locus_tag="VQM60_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080318.1"
                     /translation="MFSLSRESEQDLAHGILEVVERYLEAREKVPPRLTKLINRVELK
                     EELKISDNTLNKWESQGLRRYQPPDDGSRMIYYLVTDIWKFLGVDE"
     gene            9655..10521
                     /locus_tag="VQM60_000423"
     CDS             9655..10521
                     /locus_tag="VQM60_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HET1080319.1"
                     /translation="MTIYEARGFQSNLVYPFDKIEPFQYIERFKPLVVPEGADIEEYK
                     RTQAPYCISGKVTPEKHGSYKRNNSSLIYRDLIFLDYDDIQGTSEGFIKAISSALFGY
                     SYILYPTIKHSLEKPRFRLVVKPDNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPASYQKIVEHGLDYPVPQTTAEPVKQNAASLPYTPRPSGQKSMTMRIIDT
                     LFNGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTF
                     SSIARAEYRKRG"
     gene            10574..12043
                     /locus_tag="VQM60_000424"
     CDS             10574..12043
                     /locus_tag="VQM60_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HET1080320.1"
                     /translation="MEQEHPPRTMKELENRIFKAGEEWRAEHTETKVNENTGDVTEKV
                     AMPQTFTVAKILSEIVTFTFISKSNIPDYSLLYIYDLDEGIYTASNDLFNLLCKTFDV
                     RIKPREWPQIKLMVRTLAKIRKPLESANLIPVQNGIINLETKELLPFSPKYVITSKIS
                     TAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAIDPNHTRNKFAIFYG
                     DGNNGKGTFQRFLINLIGESNISALKPAQFAEKHNLETLVGKVCNIGDEAPNEYLKNP
                     SDLMSITSGDTVLVNPKGRPAFEATFKLFNIFSGNYIPNGGNKTKGWYRRIMIVPFNA
                     DFNGEKEKPWIKNEFLANKEVLEYALYKAINQEPFTHFIEPKAVKGLLEEYQEDNDYL
                     LSWVKHEYMEKGWHELDVVPVFIVTRSLKHYAEDMGIPKPNVYGAGKETIKHLQQLTP
                     NRYSLRKKRVSPEDFEKLEAWDVDKIKMRTPQHSITKQE"
     gene            12396..12563
                     /locus_tag="VQM60_000425"
     CDS             12396..12563
                     /locus_tag="VQM60_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080321.1"
                     /translation="MKVKLFEYTRRWEKEDFEKEIDDFMATVEVLDVKFSGTPSSESF
                     NSVAIVLVLYR"
     gene            12647..13177
                     /locus_tag="VQM60_000426"
     CDS             12647..13177
                     /locus_tag="VQM60_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080322.1"
                     /translation="MTLKTFSSKAKTFTFTYEFKDLDTALVAGHALLGYMTGTYCQPV
                     ISLTYKDKGTLVAEYVEDHKLNKTFKRICDSFKDYHKQPGEAEAFEERYKRERVLQLK
                     ESEDFESLLNKVTDYELELLDYADRLLSDKPIPMDSMTAFATLEMLGDESISLLQKLD
                     VEGEYKGLAGYTEHLK"
     gene            13248..13757
                     /locus_tag="VQM60_000427"
     CDS             13248..13757
                     /locus_tag="VQM60_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000811078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080323.1"
                     /translation="MKQLAIEAITKPMKLRGISKGIAELDGQRLEIDLDSLMIDFGGE
                     SFELDRIAGTKGGNRYFFLCPDCGRRCRLLYKRYLYFSCGTCLDIHKHTLNRSKTDCQ
                     YYWELALKEARKVEPGWSPRRGGYMFDSFPERPKYMKQKRYYKHYQKFINYTRKGDSF
                     WLNGLSSLR"
     gene            14953..15372
                     /locus_tag="VQM60_000428"
     CDS             14953..15372
                     /locus_tag="VQM60_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HET1080324.1"
                     /translation="MTPEQVKEKLEGVKWINKEIKGLYLELETLEGGIIKKPTLSHSR
                     VQTSRENKTENNLISVLKLKEDTLQRIERLTEERMKISELIDKLANPLERSVLRLFYL
                     NDLVVSEVAEEIDRSNTTVYIIRQKAIEHLTGIVNGD"
     gene            15861..18692
                     /gene="uvrA"
                     /locus_tag="VQM60_000429"
     CDS             15861..18692
                     /gene="uvrA"
                     /locus_tag="VQM60_000429"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET1080325.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            18685..19746
                     /locus_tag="VQM60_000430"
     CDS             18685..19746
                     /locus_tag="VQM60_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET1080326.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            19878..20276
                     /gene="spx"
                     /locus_tag="VQM60_000431"
     CDS             19878..20276
                     /gene="spx"
                     /locus_tag="VQM60_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET1080327.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            20342..20911
                     /locus_tag="VQM60_000432"
     CDS             20342..20911
                     /locus_tag="VQM60_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET1080328.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            20998..21264
                     /locus_tag="VQM60_000433"
     CDS             20998..21264
                     /locus_tag="VQM60_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET1080329.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            21268..21687
                     /gene="ruvX"
                     /locus_tag="VQM60_000434"
     CDS             21268..21687
                     /gene="ruvX"
                     /locus_tag="VQM60_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET1080330.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            21703..22008
                     /locus_tag="VQM60_000435"
     CDS             21703..22008
                     /locus_tag="VQM60_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET1080331.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            22266..23516
                     /locus_tag="VQM60_000436"
     CDS             22266..23516
                     /locus_tag="VQM60_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET1080332.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            23513..24055
                     /locus_tag="VQM60_000437"
     CDS             23513..24055
                     /locus_tag="VQM60_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET1080333.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            24201..25733
                     /gene="cls"
                     /locus_tag="VQM60_000438"
     CDS             24201..25733
                     /gene="cls"
                     /locus_tag="VQM60_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET1080334.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            25811..25954
                     /locus_tag="VQM60_000439"
     CDS             25811..25954
                     /locus_tag="VQM60_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080335.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            25991..27529
                     /locus_tag="VQM60_000440"
     CDS             25991..27529
                     /locus_tag="VQM60_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET1080336.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTNQGDQAGQLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVEVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            27649..>29710
                     /gene="nrdD"
                     /locus_tag="VQM60_000441"
     CDS             27649..>29710
                     /gene="nrdD"
                     /locus_tag="VQM60_000441"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET1080337.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKT"
BASE COUNT         9260 a         5460 c         6392 g         8598 t
ORIGIN      
        1 tactgtaata tgtgatgaaa atgccagtaa tgataccgag aaaaaagctg agaaactttt
       61 cccagcttta tttgttatag tcaaagagaa tgacttgttc ctgtgcatct acatgagcat
      121 ggaccccaaa gggtacaatt gctcttggag ttgcgtggcc gacattcaga ttatagacaa
      181 tcgggatatt gctgtcaatg atatccaata gtgcctcttt atagtcgtca tggaaagttt
      241 catccatagg ttttccgacc aagagtccat tgatgaccgc gaatatgcca gtgtccttta
      301 aagttagcaa catctttttg aagtcttctg gcttaggctt ttcttcgctt gtttcgagca
      361 agaggatttt cccttcccag tctgacaagt cagggaaaag tttgtatttt tggcagagtt
      421 ccgtgctatc tgcgtatcga gagttgtcaa agatatcgta gagggattcg aggcaaccac
      481 cgaggatttt cccctcgaac tgagcacttc cttgcaacaa gtcaaaacct gtatttgtat
      541 gactgacacg aggtgttccc agggccgtgg gactaaaatc agttcgttcc tcataccaaa
      601 cgtcactagg gcggatttct gaaattcttc ccgtctcaat caattcttta aagtagtgaa
      661 ggctataggc tagcatttct ttgtctaatt cacaaatgtc tgctaaaaag gattgaccat
      721 aaaaagtctt gattcctaat ttatgcaaca tgaggtggtt catggttgta tccgagaagc
      781 caagaaaaat tttttgtttg ataacctttt ggagttggtc attttcaaaa agataaggta
      841 gcaagcgata ggtatcgtct ccaccgatgg cacataggat catgtcgatg ctatcatcag
      901 aaaaggcatg aatcaaatcc tctgcacgag cttcaggatg gtccttgata aagtctaatc
      961 cttttaacga atggggcaaa aagatgggat tgagtcccag atccttgaga cgttggatac
     1021 ccaagtccac ttcgtgtttg acaaaatcct ctccgataat gccactagat aaactaacaa
     1081 taccaatagt agaaaccata tctcatcctc ctagaaatag attgagccta ttttatcaca
     1141 aaatataaga gaaagctatg tgggatttca gaagaatact tttaatactc ttcgaaaatc
     1201 tcttcaaacc acgtcagttt tatctgcaac ttcaaagctg tgctttgagc aacctgcagc
     1261 tagcttccta gtttgctctt tgattttcat tgagtacaac tcaaaaaagt aagtgaaaaa
     1321 gcaaccgcac ctgcagttgc ttttatcgtt ttcttattct tgatagcgtg ttggtcctgc
     1381 acttgcacta cggatattca agcgtttctt gagatagaag cgaagggcta ggagggctgc
     1441 tccaataata gctagtggca atggatccag tactgggtta aggctagctg gtaggaagct
     1501 tgttgcaaag aagacaagca accaaaggaa catagaagct aggataacta gtacagattt
     1561 ccagaaaggt ggacgttgac tgcgatccat atctggtcca tagtattggt aaacaaagta
     1621 gtacatcaag tagaaggcaa atccaccaac cagtccaact aatagaagag taatcaatcc
     1681 atagccgaaa gcttgatctg ccgcaaagaa ggttgtgagg gcgctgacaa gggcaaagag
     1741 gctagtgatg aaaagagctg agtccataat catcagtttt ggatcatcat tttcttttgg
     1801 atgctctttt tcgtactgct ctttgacagt gaagctatga gcccaatgag ttggtgcgcc
     1861 gtatagggaa cgggcagttg tacctttaga ttgctcctca aggatttggg gaatgacttc
     1921 ctcaaaaata gtctggattt cagcgtctgt tttcccatct ttgatgaatt gttgggtagc
     1981 aatgtggaca aactcttggt ttttcttagt taatttttgt agatcaattt gagacatagg
     2041 aactcctctt agaaccattt tttatggatg agatagagag tgagcgagac actcatagca
     2101 aaggcgataa agacgattaa ccagaaggca tttggctctc cgtttagggg aatttcatta
     2161 tccttaaagt tcatcccgta ggcagaaaag accatggttg ggatggacat gacgatggtc
     2221 acaagggcca aggttttcat gatgttgttc tggttgttag aaatgataga ggcaaaggtc
     2281 tctgtcatag aatgcaagac gtttccataa atatctgcca tctcgatggc ctgttgggtt
     2341 tcaatcaggg tgtcttcaag caggtcttcg tcctcaaggt atttcttgat attgctggtt
     2401 gaactggtca atttcttaat cacgcgctca tttgttttga gggaggcctt gaaatagacg
     2461 atagtttttt ccaattccat gagctcaatc aattcttcat tacgagttga ttgatgcagt
     2521 tgactttcga tttgttcact cttgcggtcg attgaacgaa gggctgttag gtaaagctct
     2581 gcattgcgat aaagaatttg aaagataaaa cgtgaacgca tgaaggtata gaaattacgc
     2641 aatcgacggt tgataaagac atcaaggaca ggtagtggtt ccaaacacgt agtgataatg
     2701 gtttcctcag tgatgataat accaagcggg atggttacgt agtaggtgcg gttatttctt
     2761 tcctccgtga ccggcacgtc tacgataatc agggtatact cgtcttcaat ggtaatacga
     2821 gacatttctt ccgcatcgag cggtgctcga aggtcggcaa tatcaatatc gaaggtgttg
     2881 gcgatttcga gtgattcatt ttgagtcgga ttgacgagat tgatccaagt acccggttca
     2941 agcgtatcga tctctttaaa ttcagttgtt gtagagagaa aaacttgttt catatccctt
     3001 atcctttctc atttttcaga ttttttcaca ctgtactatt atactacaaa atcggctttt
     3061 ttcaaataaa atcttacaaa gacaccgttg ttattggatt tttactttgt cttgaaatgt
     3121 tgatttttaa atttgctccc ctttttgctc cccctatagt gcttttagtg cagtttcgta
     3181 gaatgatact gctttttttg cgttctcttt tgagaggtga ctatatatat ccatggtcat
     3241 tgataaagtg gaatgaccta ggcggtactg gagttccttg tatggtattc cagagttaag
     3301 caagagacta gcgtgagtgt gacggaatcc gtgaaatcca atgttaggaa catcagcacg
     3361 cttaaaatgt gtttgcaatc ttcttttaag tcttgaactg ctaggatact catgaatgaa
     3421 atcagaaaat accacgcgtt cacgttgccc tatcttccag gcctctttag tttggcgtag
     3481 tttgtactgc ttcagcatgc taacagtcgc ttgatctata tctatatccc gataactagc
     3541 cttagacttt gggctattga tttctatatc ccgatttagt gttttggtaa tatgcacaac
     3601 ggcattatct aagtcaatat cagaccagga aagagctaaa gcttcattga tacggcaacc
     3661 agtagccaat aggaacttat agagtgttgt ctcatagtag taacgatatc tatgagtatc
     3721 taaattatcg agataaccaa gaaatttttt agttcttgat tttcaaagtg ctttactttt
     3781 ttccgctttg ctttctgagt gttacgaggt aaaacaacat cacgcgcagg attggacggt
     3841 atagcctgca tggttacgcc atactgtaag atacgtttgt ttaaagcgtg tatcttgtca
     3901 taatgtagaa aagccgcttt ttcccctcta ttagtcttgt cagcaagttc gttgataatt
     3961 gactggataa gtggagtagt gagcttatcg agcttgtaag ccccaaaaat aggcaatata
     4021 tgattatcga ttagcttttt aacattgcct tgagtgtttg gcttaaccgt attcttataa
     4081 ctttccaacc agagagttgc caattcttga tagtttttta tggtaacagc cttttgtctg
     4141 gtagatcctg ctttttgaaa agctattttt tcttgattgg ctttctgctt aacttccttt
     4201 tgtgtccgac ctgttacttt ggtttttact ttctttcctg ttacctggtc aactcctaga
     4261 tatacattag cacgataaac tttgctaccg tcttttttta taacttcagt tattttcatg
     4321 atgataaacc tttctaaaat acatcagcag gcaagccatt atcaaaaagg ttttatatca
     4381 tgctaggagc tacgagaacc cccttatttt cgttttattt cttccagtaa agaatacata
     4441 gcattaatgt ggttatattt ttgaaattca ggatcttggt tttgtgtaat aaagtatacg
     4501 tattgtctac cagatttgaa tttatcgata gcttgttttt ccactaattt ttttaaatat
     4561 tttaaaattg tatttttgtg gatttttagt ttttcttcca aagcctctct tgttacgaaa
     4621 cggtgttcga ttattaactc taatatcttt ttctcatatt cgttatcaat atccagatta
     4681 tctataaggg aagtatccca taatgtaaat tcaactgagt ctaattcagt tcgtaaattg
     4741 ggatatttta aatgactctc tttaactgct tccattattt tgggtatacc agttcccgct
     4801 ttttcacata aatttaaaaa tctaaaaatt tcttgtatta agtagtttct tggatcagaa
     4861 tgtgccccac taaaaaagtc ctttatgtca attctaagtg ttccgccatt tgtgaatata
     4921 tacctgtcgg gatatctaat tatcattatc cctttttcgc ttttatagtc gctatgaata
     4981 aggctattaa tgaatgcctc acgaattgca attcgtaatt ttgaaattcc aattctattc
     5041 atagaatttt cttgaatgtt agagttgtca tttaaagtta gatataattt ttcaataact
     5101 aggtaaaaga aattaaaaag attatcttcc ccccatgtac catcataaat cacacgatct
     5161 ttaaagcgat tgttttcaga aaactctttt agaacatatt ctacgttata gtgtggaata
     5221 aactctttta ttgaattatg tgtaccaaaa attaatagtc cagcaactgt tggctttatt
     5281 ttatcgtcct tgcgatctct tcgtaaagca tttattttta ttaaaaattg ttcgttatcc
     5341 aactttgaaa aagggtgctc cgcatttata ttatcaaatt tttctctata tagctttatt
     5401 gtttctgtat ctaagtctaa aatagaaaaa tcctgaataa gtgtgctatc aaaactttcc
     5461 ttagcggaat ctcttaacat agagttgatt tgctcttgtg aacacctaaa atcaccagtc
     5521 ccctgacgaa aataggtaga tgctatgttg tcattaagat atataggttt atccttgtag
     5581 tctaccttgt taacagggat aatgacaatc tcttttcctt ttatatttac gacttcaaca
     5641 ctttcttcat taatcacatt tctattaatt ttattagggt tattaagtaa actatataat
     5701 tcgtccagta ctttatcggg atcattaact ccagaaatat aaaaatcttt attctttgta
     5761 ttctctgaga cacctagaat taacagacca ttatttgtat tagcgaagga acaaattgtt
     5821 ttcataccgt ctttagggaa agaattttta gcttctttga attcaacacc ttggttctcc
     5881 cctgataata acaattttgg tatatcgtat tttctcattt tttctccaat caatttgcac
     5941 acgcattcgc acacgcgcgg gaaaaatagt ttatttttag ttagctaaca gtgctattaa
     6001 aggttttttt gaaagtagat tattcgcaca cgcattcgca cacgcgcttc aggattgttg
     6061 aacattgtgg cgcgtgggtt agtctatacc ccaaagtaac gcaaaatcat ctagattttc
     6121 tttatctttc ttagcttctt gaagagctaa agacttaatt tcttcaaata gattttcaaa
     6181 ctttttatat aatttgttgt aatattcttg gttgaattcc ttatcttcaa aaactatgta
     6241 gaattcacta cttgtttttt gaaatacaat atctagccag aataggatat tacttgtctt
     6301 tagcgcaagg gcatttaaaa cttcatagtt gtctttggga ttgtattcat ttaatgattc
     6361 agtgatagta gtgagaaact cttcaggact gattgggaaa tttttactct ttgctagttc
     6421 aattatttcc ttgtctcttt tctctctctc ttttactttc tgaaaaattt tatcttttga
     6481 aacatttttt acgaggtatt tttcttcgtc taattgttta tctaacatat ataaaaattc
     6541 tttgtaaaca tctttgtcag aaaagcgagg gtcaagatca gattttttca ctcccaaata
     6601 atcagcaagt ttttgaagat tgccagatgt tggcattgat ctacctttta cataaccagt
     6661 tattgtactt ttaggaattt ctaaagcatt atgcaggtct atctgtctga tacctttttc
     6721 tttcataaca cggttaagat tattagagaa atattctcta ttctttaaat cctgagggct
     6781 attttttgcc attgttaaaa actcccttac tttaattcta caacctgatt ataacacaaa
     6841 aaagacaaag tacgaaaaaa ataaaatttt tcaacaacag cgttgataaa gtacgaaaaa
     6901 aatagtacca tgattttatc aatcttgaaa ggagctataa gaatatggca caatggacgc
     6961 taagagcgtg tcgagtaaat gcaggtttta ctttgcgcca agtggctaag aaggtcaaca
     7021 agaattttca gactatctca aagtacgaaa aagatagtac gctaatccct tttgagctac
     7081 ttaaagagtt atcggaactg tatcaggtaa aactggacga tattttttta ggagatagta
     7141 cgaaaaaaat agaactaaat caaaaggaaa tctaagaaag gagcaaacca atggaattag
     7201 tctacatgga cggtaagaaa gagccgtata cactgagcag tattgttgcg gagtgtgcag
     7261 aagttaagca cagacatttg aagattttgc tgaataaaca tcgagaggac ttcgagagtt
     7321 tcggaaaggt gcaatttaaa atttcacctt cagaaagtgg gcaaaatgta cgggactata
     7381 ttttgaatga gcaacaagca accttgctga tcacttactt acgaaataca gaacccgtaa
     7441 aagagtttaa gaagaacctg gtcaaagcat tctttgaaat gcgtgaggag ctttctaagt
     7501 tccgtatgca gagggcgcta gaaaagccaa aaagaaaaac cttgcatgac agtatagaga
     7561 catgggaggc aaaacccaag cacgcgcata gcaccatgaa caacctgcta ctaaaagcag
     7621 taactgacat gaacgctaag cagttaaggg aagaacgtgg gggctataac ggtattgata
     7681 gcttgaccag tgacgagctg gagcaatacc aggcatttga ggatatggta atagccatga
     7741 tcggcttgaa tatgagttac caagaaatca aggctatggc attcagaaat aaaaaaacac
     7801 gccaaagaag cgcgtgagag caacaaaaaa ggcttagcaa taaccacacc gcagagcctt
     7861 tcacactaac actaaaacga atttaacaaa gcaggcaagc tattatcaaa gaggttttag
     7921 taaagattta tacctagatt atagcatatt tagggcaatt tgaccatacg gagagcgcca
     7981 actctttaaa ctggtacttt ctcatgctct caatttggcg actcagagcg tgaggctatg
     8041 aattgcaaga aaaactgtat aagaaacaac cattaaaaac ccccacactc tcggtcggca
     8101 aacttctgag cgtgaggata tccagtatag taaaaggcat taaaagctct tagagcaatt
     8161 acacaacata gaaacaaagg taaaaaacat gacagaaaca acatacgaga atttaactag
     8221 acatatagac agaattagcg cagaaatgcg agaaattggc gaaaaaaatg ggcttagtag
     8281 actttcatta ttggcaaatc agacaaaatt catcaaagaa aatttacacc gtttactttg
     8341 gattgaactt cctgaattga atgacagcca taaaatcgag gcagtttcta aaagcactac
     8401 gggaatgttt ttccacgctg gtatttttga aatggacgct atgcgacaag cattctttaa
     8461 acgccaagct atgcactttt ttgacaacga agcagagcaa caggcgtata tagaacatgc
     8521 tgaaaaggag tatttagagg ctactataac cttaaaagat attcttttta actctaaaaa
     8581 tggaactcag agagtaaata aaagttgtct tatagagaag tttgaggagg caatgcaatg
     8641 acactaaacc tagacaacat gacacaagca gagtttgatg aagtaatggc tgaaattaag
     8701 gaaaaacgcc ccaacctctt ccagtttatt gctgattttg tagatcgaaa agtaagcact
     8761 gaggaggtag acgaatacct gaagatgaaa catgaaaaac aagtggaatt tatcaagaat
     8821 tatcaagcga gggcataaca tgaatgagtt agatttgacc aatacacaat cggtaatctt
     8881 tatggtggta ttgattggct tactgcttta cctaaaccac cgagaccgca aaaaaagcgc
     8941 ccaatttgag cgagaaaacc aacagaggat agaaacacct agcgaggatt taagccctga
     9001 ttatgggcgt tatatccagc ttgcaggggt aagagtttac ggaggaactg aatgagtcta
     9061 tcagagaatg acaaacgagt attaagacta atcaaggtag gggctgagaa ctccataaca
     9121 ggggcagaaa tcagccttat aactaagctg gcggaaagaa ccgtacaaga tattatcagc
     9181 cgtctaatca cgcgtcatag tgttcctatt gttggggttc gccatgggac atttagagga
     9241 tattttatcc cagctaacaa agaggagcta ctggacggag caaaggcatt ctataaccaa
     9301 gtacaggaag aacagaaacg cctaactgtc cttttgaatg ctgatttaga gagttacaaa
     9361 gaaaccttga aggaggtggg cggatatgtt tagcctaagc cgagaaagtg agcaagacct
     9421 agcgcatggc attctggagg tagtggaaag atacctggaa gcgcgtgaga aagtaccgcc
     9481 aagattgacc aagttaataa atagagttga actcaaagag gaattgaaaa ttagtgacaa
     9541 cacgctgaat aagtgggaaa gtcagggttt aagacgctat caaccgccgg acgatggttc
     9601 aagaatgatt tactatttag taactgacat ctggaagttt ctgggggtgg atgaatgaca
     9661 atttacgagg ctagaggttt ccagagtaat cttgtctatc catttgataa aattgagcct
     9721 ttccagtata ttgaacgatt caagccctta gtagttcctg aaggggcaga tattgaggaa
     9781 tacaagcgaa ctcaagcgcc ttactgtata agtggcaaag tgacgccaga aaagcatggc
     9841 agttacaagc gaaacaactc cagtctaatc tatcgggatt tgattttcct tgattatgac
     9901 gatatacagg ggacgtctga gggctttata aaagccattt ctagcgcttt atttggctac
     9961 tcttacatct tatacccgac tatcaaacat agcttagaaa agccccgttt tcgccttgta
    10021 gtgaagcctg acaacgtgat gaatgaggcg acctataagc aggtagtgaa agagattgct
    10081 gacaagattg gactcccctt tgatatggcc agtttaacat ggtcacaact ccaagggcta
    10141 ccagtcacaa caggcgaccc agcaagttat caaaagattg tagagcatgg acttgattat
    10201 ccagtaccac agacaacggc tgaaccagtc aaacagaatg cggcaagcct tccatacact
    10261 ccaaggccaa gcgggcagaa atctatgacc atgaggatca ttgacacgct tttcaacgga
    10321 tttggagacg agggagggcg caacgtagca ctgacacgat ttgtaggctt actctttaat
    10381 aagtgggttg attgtgacct agagacagcc tatgaactta caaagatagc caatagcgtg
    10441 acagttgaac cattgccgat tgaggagcta gaccgcactt ttagcagtat agcacgggct
    10501 gaatatagaa agagaggtta gaaccataga actagaagaa ttagcaaacc taaaaagtga
    10561 aatcttggag gtaatggagc aagaacaccc tcctagaacg atgaaggagc ttgaaaatcg
    10621 tatctttaaa gctggagaag aatggcgggc agaacatact gaaactaagg taaatgagaa
    10681 cacaggggac gttaccgaaa aggttgccat gccccaaacc ttcacagtag ctaaaatact
    10741 gagcgagatt gtcacattta cttttatcag caagagcaat atacctgatt atagcctact
    10801 ctatatctat gatttagacg agggtatcta taccgctagt aatgatctat tcaatctttt
    10861 gtgtaagacc tttgacgtta gaattaaacc cagagagtgg ccacagatta agttaatggt
    10921 tagaacattg gcaaagatac gaaaaccttt agaaagtgct aacctcattc ccgtacagaa
    10981 cggcattatc aacttagaaa ctaaggaact actccccttt agtcctaagt atgtaattac
    11041 aagtaagata agcacggcct accacgcgcc taaacgagtc ccaacagata gagagggaaa
    11101 aacttttgat gattggttga actcaatcgc ttgtaatgat agtgaactgg taacactgtt
    11161 ttggcagatt atccttgaag ctattgaccc taaccatact cgaaataagt ttgctatctt
    11221 ctacggggac ggtaacaatg gtaaagggac atttcagcgc ttccttatca acctgatagg
    11281 ggaaagcaat atatcagcct taaagcctgc acagtttgct gaaaagcata acttggagac
    11341 cttggtaggt aaggtttgta atattgggga tgaagcacct aacgaatact taaaaaatcc
    11401 gtctgattta atgagtatca ctagtggcga cactgttcta gtcaatccaa aaggacgccc
    11461 agcctttgaa gctactttca agttattcaa tatcttttca gggaattata tccccaatgg
    11521 tggcaacaag acaaaaggct ggtatcgtag aattatgatt gtccctttta atgctgactt
    11581 taacggggag aaggaaaaac cttggataaa aaatgagttt ttagcaaaca aggaagtcct
    11641 agaatatgcc ctctacaaag ctatcaatca agagccattt actcacttta tcgaacctaa
    11701 agcagttaaa ggactcctag aagaatacca ggaagataat gactacttac tttcatgggt
    11761 taagcatgaa tacatggaaa aaggttggca tgagctggac gtagtacctg tttttatcgt
    11821 tacaagatca ctaaagcact atgccgaaga tatgggaata cccaagccca atgtttacgg
    11881 ggctggtaaa gaaactatca agcacttaca acagttgaca cctaatcggt attctctaag
    11941 aaagaaaaga gtttctccag aagattttga aaaactagag gcatgggacg ttgacaagat
    12001 aaaaatgcgg acacctcaac actctatcac taaacaagaa taagtgtgac gttctttttt
    12061 tgcttaaacc cttgataata ctgattttct tgaaaaaagt gtgacgttgt gtgacgttct
    12121 caaaaaagaa ggtcacaccc cttgaagcct tgatatatct aactttaggg gaataggtgt
    12181 gacgttgtga cattctttcc aactcttata tatagagaaa aatacagtat ttatatatat
    12241 aggaacgata aaacaaaaga acgtcacagg gtcacagggg tggtgaaaca cctagagcct
    12301 caagggtttg aacgtgtgac gttctttgtc acaaaaggtc acagaaaacg aaaaatatta
    12361 cataatttac cttaaaacaa agaaatgaca acaacatgaa agttaaacta tttgaatata
    12421 ctagacgatg ggaaaaagaa gactttgaga aagagatcga tgacttcatg gcaactgttg
    12481 aagtgctgga cgttaaattt tctggtacac catctagtga atcctttaat tcggtggcta
    12541 tcgtattggt attatacaga taacaaaatg gggtagatag taccgacccc cttgcaatgg
    12601 tgtaactact agtgacaccc ttaaaaacag aaagaggaaa aacaacatga cattaaaaac
    12661 attttcatct aaagccaaaa catttacttt cacttacgaa tttaaagacc tagatacagc
    12721 tctggtagca ggtcacgcgt tactgggata tatgactgga acatattgcc agcctgtaat
    12781 ctcattgacc tacaaggata aaggcacgct agtagctgag tatgtggaag atcacaagct
    12841 aaacaagacc tttaagcgta tttgtgacag ttttaaagac taccacaaac aaccaggaga
    12901 ggctgaagca tttgaagaac gttacaaacg ggagcgcgtg ctccaactca aagagtcaga
    12961 ggactttgag agtttactga ataaggtcac agactacgag ctggaactat tagactatgc
    13021 tgaccgcctg ctttctgata agcctatccc tatggactct atgactgctt ttgctacgtt
    13081 ggaaatgctg ggcgatgaaa gtattagcct actccaaaag ttggacgtag agggcgaata
    13141 taaaggcctt gctggttata cagagcattt gaagtagaat acaggggagc aatccccttt
    13201 ttgctatcaa gtcaatagtt ttgggttttt ccatataggg ggaaataatg aagcagttag
    13261 caatagaagc aatcactaaa ccaatgaagc tgagaggaat atctaagggt atagccgagt
    13321 tggatggtca acgtttagag attgatttgg atagtttaat gattgatttt ggtggagagt
    13381 catttgaatt agacagaata gcaggaacta agggaggtaa tcgatatttt tttctttgcc
    13441 ctgattgtgg tagacggtgc aggttgcttt ataaacggta tttgtatttt agttgcggta
    13501 cttgtctaga tatccacaag cacaccctga accgcagtaa aacggattgc caatactact
    13561 gggagcttgc actaaaagaa gctagaaagg tagaaccagg gtggagtcct agacgtggcg
    13621 gatatatgtt tgatagtttt cctgaaagac caaaatacat gaaacagaaa cgatattaca
    13681 agcattatca aaagtttatt aactacacca gaaaagggga tagcttttgg ctgaatggtt
    13741 tgagttctct aaggtaattt gtagtagtgt gcaatttaca aaacaaacaa atactacata
    13801 ttagacggtt tgaatgttaa aaaagctaga tatatcaagg atttaattaa gtatagcgtt
    13861 ccaaaaaaag ggtgcagtaa gcgttccaaa aaagggtgag gttgaatatt taaaaaaggc
    13921 taagattagg ctaaggtcac atacttcaaa atagcaaggt tttagcaagg tacagggctt
    13981 ttttctagtg atagaacccc aaggttacca caaggtatag ctactttttt agaaccatag
    14041 aacggtaagg ttacggtaag gtatcgaagt tttccaatgg taaggttttg gtaaggtagc
    14101 atatataaaa agtacaaggt tagtacaagg ttacatactt aaaaatacta aggttttaca
    14161 aaggtagaga ggtttaccga gggttagaaa aggtgagatt ttgcttaggt agtggcagta
    14221 aatatgcctg acaaatggca gggttatgtg tgttgaactc cgagcgaacg ccgagaaagt
    14281 ggctatatcc cccctatcat ttatcaagta gataagagca ataaaaaccc tccttaaccc
    14341 cctgagaaac gacaagcaga taagagaagt ttttagccct cttatttcgg atctactcct
    14401 tgcagaatga caagcagata aggacgacaa taagccttct taactgctta caaaacgata
    14461 gggagttaag acgaatttaa aagcccctta ttttcgtttt tctccttgtc aaacgggaag
    14521 gaaataagac aggaaataac atcgcttatt ttgattttgc tccctgaaaa actgtaagga
    14581 gataaggaga cttattagct tccttaattt gaattttctc cttacaaaat gacagggaaa
    14641 taagaactaa aataactccc cttattttcc taccaaatga taaggagata agagcagttt
    14701 aaaagtctgt tattttggat ttgttgctta caaaatgata agcagataag caaagaaata
    14761 acttgcctta attgcttgca gaatgacaag gaaataagag agataaaaac atcccttaat
    14821 tgcttatcaa atggtatgga gaatggactt gaacctagag cgaagctaga gaaaatgaaa
    14881 aaaagccagc acagggctga ctcctttatg aattattcca taaaactatt atatcacaaa
    14941 ggaggccgag gcatgacacc agagcaagta aaagaaaaac tagagggcgt taagtggata
    15001 aacaaggaga tcaaaggctt atatttggaa ttggaaaccc tggaaggtgg cattatcaaa
    15061 aaaccaacac taagccatag cagggtgcag acaagtagag agaataagac agagaacaat
    15121 cttataagtg ttctgaagct aaaagaggac actttacaga gaattgagcg acttactgaa
    15181 gagagaatga aaatatctga gctaatcgat aaactggcca atccgcttga gcgttctgtt
    15241 ctaagacttt tttacttgaa tgatcttgta gtttcggagg tggctgaaga aatagataga
    15301 tcaaatacta cagtatacat tataaggcaa aaagctatag aacacttaac aggtattgta
    15361 aacggggatt gatttaacct taccacggaa ttaggagagt atgggggagt cctaagccac
    15421 atattcgccc gtatttcgct ttgtttcgta ctggacaact aaaacaccat ggataaacta
    15481 caaaccctca gaaacgaatc tgggggcttt tgtgaggtca taatcttata aaccacgcgc
    15541 ttcatggtat aatatatcta tcagcaacca gcaataaaaa agcacgtttg accgtgctag
    15601 tttcttgcct gctgaactcg tcaaatctta gcccttttgt ggggcttttt tgctcccctt
    15661 tttgcgtact ttgggggagc aagtagtaaa aagtaatgtt agtaattttt attaaaaagc
    15721 gcgtgatata agggttagga agccctaagg aaacataaaa taagcgattg ttgattatac
    15781 tacaaaatcg gccttttggg taatagaatc tcactttttt tggtataatg gtaagcaata
    15841 atggactaga aagaacaaag atgcaagata aaattgtcat tcatggggcg cgtgcccata
    15901 atttaaaaaa tattgatgtg gagattccgc gagacaagtt ggttgtcgtg actggcttgt
    15961 caggttcagg gaagtccagt ctggcttttg atactctcta tgcggaggga caacgtcgct
    16021 atgtagagag tttgtctgcc tacgctcgtc agttcttggg aaatatggaa aaaccagatg
    16081 tagatgccat tgatggtctc agcccagcta tttccatcga ccagaaaacg actagtaaaa
    16141 atcctcgctc gacggtggga acgacgactg aaatcaatga ctatctgcgt ctcctctacg
    16201 cacgtgtggg gacgccttac tgtatcaacg gacatggagc tatcaatgct tcttctgtgg
    16261 agcaaatcgt tgataaggtt ttggagttac ctgaacgcca gcgcttgcag atcttggctc
    16321 ctgtcatccg caagaaaaaa ggccaacata agagtgttat cgagaaggtt cagaaagacg
    16381 ggtatgttcg tgttcgtgtg gatggggaag tctatgatgt gaccgaagtg ccagagttgt
    16441 ctaagagcaa gcaacacaat atcgatgtcg tggttgaccg tattgttatc aaggagggca
    16501 ttcgtagccg tctctttgat tccattgaag ctgcccttcg tatcgcagaa ggttatgtga
    16561 ttatcgacac catggacgac tctgagttgt tgttctctga gcattatgcc tgtccagttt
    16621 gtggatttac tgtcccagag ttagagcctc gtctcttctc tttcaatgct ccctttggct
    16681 cttgtagtga gtgtgacggc ttggggatta agctggaggt ggatactgat ttggtagtgc
    16741 cagatgccag caaaacctta catgagggag cgctggcacc ttggaatcct atctcatcca
    16801 actactatcc aaacatgtta gagcaggcca tgaaagtctt tggagtggct atggataagc
    16861 cttttgagga cctgtcagaa gaagataaga acttgattct ctacggctca gatggtaagg
    16921 aattccattt ccattatgaa aatgaatttg gtggtgtgcg cgatatcgac attccgtttg
    16981 agggagttat caataatatc aaacgtcgtt accatgaaac caatagcgat tacactcgca
    17041 ctcagatgcg tctctacatg aatgagctga cctgcggaac ctgtcaaggc tatcgtctca
    17101 atgaccaggc cttgtctgtc cgtgtgggcg gccagcaagg gccacatatt ggagaaatct
    17161 cagacctgtc tatcgctgac cacttggact tggtgagcca gttaactttg tctgaaaatg
    17221 aagccatcat tgctcgtccc attctcaagg aaatcaagga tcgtttgacc ttccttaata
    17281 atgtgggtct taactatcta accctgtcac gttcagcagg aaccctttca ggtggggaaa
    17341 gtcagcgcat tcgtttggca acccagattg gttccaacct atcaggtgtc ctctatatcc
    17401 tagacgagcc gtcaatcggt cttcaccaga gggacaatga ccgcctgatt gccagtctga
    17461 aaaagatgcg tgacttgggc aatactctta tcgtggtgga acacgacgaa gataccatgc
    17521 gtgaagctga ttatctgatt gacgttggtc ctggtgccgg tgtttttggt ggtgagattg
    17581 ttgcagcagg tacacctaag caagtggctc gcaacagcaa gtctatcaca ggtcagtact
    17641 tgtcaggcaa acgtgtcatc ccagtaccag aagagcgccg tgtcggaaat ggtcgtttta
    17701 ttgaagtgac aggagcgcgt gagaacaact tgcaaaatgt cacggctcgc tttccactag
    17761 gaaaatttat cgcagtgaca ggtgtgtcgg gttcagggaa atcgactcta atcaacagca
    17821 ttctcaaaaa agccattgcc cagaagctca accgtaattc agacaaacct ggtaagttta
    17881 agactattac agggattgag catgtcgacc gtttgattga tattgaccag agccctatcg
    17941 gacggacgcc gaggtctaac ccagctacct atacaggagt ttttgacgat atacgtgacc
    18001 tttttgctca gacaaatgaa gccaagattc gaggctacaa gaaggggcgc ttcagtttca
    18061 acgtcaaggg aggtcgctgt gaagcttgct caggtgacgg gattatcaag attgagatgc
    18121 acttcttgcc ggatgtttat gtggcttgtg aagtttgcca cggaacccgc tataacagtg
    18181 aaaccctaga agttcactac aaggaaaaga atatttcgca ggtcttggat atgacggtca
    18241 acgatgcggt agaatttttc caacacattc cgaaaattca acgcaaactc cagaccatca
    18301 aggatgtagg tctaggctat gtgaccttgg gacagccagc taccaccctt tctgggggag
    18361 aagcccaacg tatgaagtta gctagcgaac tccacaaacg ctcgacagga aaatctttct
    18421 acattctgga tgagccgacg acagggcttc atacagagga cattgctcgc ctgctcaagg
    18481 ttttagctcg ctttgtagac gacggcaata cagtcctcgt catcgagcac aatctggatg
    18541 ttatcaagac ggcagaccat atcattgact tgggacctga gggcggtgtc ggtggtggaa
    18601 ccatcatcgt aacaggaact ccagaagaag tagctgccaa cgaagccagc tatacaggac
    18661 actatttgaa aggaaagtta catcatgaat aaacgcgtac aagcatttct agctaaaatg
    18721 caagaaaaag aactagatgg tatcatcatc aacaatctta aaaacgtcta ttatttgact
    18781 ggtttttggg gctcaaacgg aacagtcttt atcagccgtg accgtcaggt cttagtgaca
    18841 gactctcgct atatcatcgc agctaagcaa gaaaccagtg gttttgagat tgtggctgat
    18901 cgtgatgaat tggctgtcat tgcaggaatt gttaaggaca tgaacttgac tcgtatcggt
    18961 tttgaagatg agatttcagt gtcttattac caccgtatgc aggcagcttt tgcaggtttg
    19021 aacttgcttc cacaaactca gtttgtggaa ggtcttcgta tgattaagga tgaggcagag
    19081 attgcagcga ttcgcaaggc ttgttctatc tcagaccaag ctttccgcga tgcgcttgac
    19141 tttatcaaac caggaaaaac tgaaattgag attgccaact tccttgattt ccgcatgcgt
    19201 gagttgggag catctggctt atcttttgat acgatcctag ctagcggtat caattcttct
    19261 aaaccccatg cccatccaat gcacaaacca gtggagttgg gagaagccat taccatggac
    19321 ttcggctgtc tctatgacca ctatgtcagt gatatgaccc ggactatcta tctagggcat
    19381 gttagcgatg agcaggcaga gatttacaat acggttctaa aagctaacca agccttgatt
    19441 gaccaagcta aggcaggctt aggtttccgt gactttgaca aaatccctcg tgatattatc
    19501 attgaggcag gttatggtga ctactttact cacggcattg gccacggtat tggtctggat
    19561 atccatgagg aaccctactt tagtcagact tctacagaaa ctattaagac aggtatggcc
    19621 ttgaccgatg aaccaggtat ctatatcgaa ggcaaatatg gcgttcgtat cgaggatgat
    19681 atcctgatta cagagacagg ttgtgaatta ttgaccctag ctccaaaaga gttgatagtc
    19741 atttagttgt ttgtcaagaa aaaagtacga aaatgacgaa aaaagtcaaa aaaaattaaa
    19801 aataggtcgc aagtcggatg ttttttatgg tataatagac taaactgata gtaacatgta
    19861 gcgaaagggg taggtacatg attaaaattt atacagtctc aagttgtact agttgtaaaa
    19921 aagcaaaaac ctggctcaat gcccaccagt taagttataa agaacaaaac cttggtaaag
    19981 aaggaattac gagagaagaa ttactggata ttctaaccaa aacagataac ggaatagcca
    20041 gcatcgtttc gtctaaaaat cgctatgcca aagcccttgg agtggatatt gaagatttga
    20101 gtgtcaatga agttctcaat ctgattatgg aaacaccgag aattttaaag agcccaatcc
    20161 ttgtagatga aaaacgcctg caagttggct acaaggaaga cgatattcgt gccttcctac
    20221 cacgctctgt ccgtaatgta gaaaatgcag aagcacgttt gcgtgcagct ctataaacat
    20281 caaggctggg agcacttccc agtcttattc tattttaatt tcaaaaagaa agaagaaaga
    20341 aatgaaaaaa atagttcttg ttagtctagc tttccttttt gtcctggttg gttgcggaca
    20401 gaaaaaagaa actggaccag ctacaaaaac agaaaaagat acgcttcagt cggcattgcc
    20461 agttattgaa aatgctgaga agaatacagt tgtaactaag actttggtct tgcccaagtc
    20521 agatgatggt agccagcaga cacaaacaat tacttacaaa gacaagactt ttttgagtct
    20581 agctatccaa caaaaacgtc cagtctctga tgagttgaag acttatattg accaacatgg
    20641 agtggaggaa actcaaaaag ctcttcttga agcggaggag aaggataagt ctatcattga
    20701 agctcgtaaa ttggcaggtt tcaaacttga aacaaaacta ttgagcgcaa cggaacttca
    20761 aacaacgact agttttgatt ttcaagttct ggatgtcaag aaggcttccc agttggaaca
    20821 tctgaagaat attggtttgg aaaatctttt gaaaaatgaa ccaagcaaat atatttcaga
    20881 tagattggca aatggcgcga cagaacaata gaaaatccaa ataaatagac tgcctgaatt
    20941 gtagaaggta aggaattatg ctataatggt actattctaa ggaaagaggg tgttagaatg
    21001 ggatttactg aagaaacagt acgttttaaa ttggacgatt ccaataaaaa agaaattagc
    21061 gaaactttga cagatgttta tgcttcgttg aacgataagg gttacaaccc aattaaccaa
    21121 atcgtaggtt acgtattgag tggagaccct gcctacgttc ctcgttataa taatgcacga
    21181 aatcaaatcc gtaagtatga gcgtgatgaa atcgttgagg aattggttcg ctactacctc
    21241 aaaggacaag gagtcgatct ataacctatg agaattatgg gattggacgt cggttcaaaa
    21301 acggtagggg tggcgattag cgatccgctt ggttttacag ctcaagggct tgaaatcatc
    21361 cagataaatg aagaacaagg ccaatttggt tttgaccgcg ttaaggaatt ggttgatact
    21421 tacaaggtgg aacgatttgt agtgggcttg cctaaaaaca tgaacaatac aagtggaccg
    21481 cgcgtagaag ctagtcaagc ctacggagca aagctagaag agttttttgg tttaccagta
    21541 gactatcagg atgaacgctt gacaacagtg gctgctgagc gcatgttgat tgaacaagca
    21601 gatatcagtc gcaataagcg caagaaagtc attgataagt tagcagctca gctgatttta
    21661 caaaattatt tagatagaaa attttaatat aaaggagagg ctatgtcaca cgatcataac
    21721 cacgaccacg aagaacgtga actaatcaca ctagtagatg agcaaggaaa tgaaaccttg
    21781 tttgaaatcc ttttgacaat tgatggaaaa gaagaatttg gtaaaaacta tgttcttcta
    21841 gtaccagtta acgcagaaga agacgaagac ggacaagttg aaatccaagc ttactcattc
    21901 attgaaaatg aagatggaac agaaggcgaa ttgcaaccaa tcccagaaga ctcagaagac
    21961 gaatggaaca tgattgaaga agtcttcaac agctttatgg aggagtgata cagtctggga
    22021 gactgtatca gcccaactcc agaaatgggg aagagttgga acgtccagtg aacattttta
    22081 ctccaactct tagaaattga gaagagttgg aacatctagt ggatgttttt agcccatatt
    22141 cacattcata gtagctagtt attagctaac caggtaagag gtcgggacga aaatcctggc
    22201 cttgttttta ttagtaacca tactttaatg cgataattga ttggactttt gtcaaggaga
    22261 tgtgtatgtt tgaagtagaa gaatggctcc atagtcggat tggtttgaat tttcgatcag
    22321 gtttgggccg aatgcagcaa gcggtggatt tgttaggaaa tcctgagcag tcttacccta
    22381 ttatccacgt aacagggact aatgggaaag gatctaccat tgcttttatg agggaattat
    22441 ttatggggca tggcaaaaaa gttgcgacct ttacctcccc tcatatcgtc tctatcaatg
    22501 accgaatctg cattaatggg caacctatag cagacgcaga ctttatccgt ttgactgatc
    22561 aggtcaagga gatggagaaa acgcttctgc aaactcctgc ccagttgtcc ttttttgaat
    22621 tgctgacctt ggttgctttt ctttatttta gggagcaaga ggtggatttg gttttattag
    22681 aagtgggaat tggtggctta cttgacacga ccaatgtggt aactggagag cttgctgtca
    22741 tcacctccat tgggcttgac catcaagaaa ccttgggtga tagtctagaa gcaattgcag
    22801 agcagaaagc tggtattttc aaggctggta aaaaggcagt gattgcgaaa ttgcctccag
    22861 aagctagact tgtctgtcag aaaaaagccg aatctttagc tgttgacctt tatcaggcag
    22921 gtcaagattt ttcagtgctg aatggtgatt tttcaagctc tttactaaat ctttcacagc
    22981 tgaacatagg cttagaagga gcctatcagc aggagaatgc ggccttggcg ttgcaaactt
    23041 ttcttctttt tatgggagaa agaaaggaag ctattgatga acaggctgta agaaaggcct
    23101 tggaacagac ccattgggct ggtcgcttgg agcgtattcg cccacagatt tacttggatg
    23161 gtgctcataa cctccctgcc ttgactcgct tggttgagtt tatcaaagaa aaagagcagg
    23221 aaggctatcg acctcaaatc ctctttggat ccttgaaacg taaggattat caagggatgt
    23281 tgggttatct gactgaaaat ttacctcaag tggaactcaa aatgaccggc tttgactatc
    23341 aagggtcttt ggacgagaca gatgtaacag gttaccatgt aattccctct taccgagaat
    23401 ttatcagcag tttcgaagaa agggcagacg ctcaagactt gctgttcgtt acagggtctc
    23461 tctattttat ctcagaagta cggggctacc tgctggaccg tgagcagata aattgacccc
    23521 cttttttgaa cttgttatac taggggaatt gctggttaga gaaaatttct ctaaattggt
    23581 agcagaaagg aaattcatca tgaaattaaa aagattcaca ctttctcttg cttctctagc
    23641 aagttttagt ctcttagtag cttgttcaca aagagctcaa caggttcaac agcctgttgc
    23701 tcagcaggtc caacagcctg ctcaacagaa taccaatact gcaaatgcag gaggtaacca
    23761 aaatcaagcg gctccagtac aaaaccaacc tgttgctcaa ccgaccgata ttgatgggac
    23821 ttatactggt caggatgacg gagaccgtat cactttagtg gtaactggaa cgactggtac
    23881 atggactgag ctcgaatctg acggggatca gaaagtcaaa caggttacat ttgattcagc
    23941 aaatcaacgc atgattattg gcgatgatgt caaaatttac actgtaaacg gtaatcaaat
    24001 cgtcgtagat gatatggata gagacccatc ggaccaaatc gttttaacta aataagacta
    24061 tgcaagtaga aagggctgga tttttcagcc tttttacttt tacctagaaa ataatcataa
    24121 atacttgcct tctaccgaat acctgcgtta tactagtatc aattacttgt ttttagcatt
    24181 caaactatca aggaggggat atgaaatata ggaaatttca attattgatg tccaagtatg
    24241 gctttagtct ttcgattatg ctacttgaac tttgtcttgt ttttggtctc tttctttatt
    24301 taggacgcat ggctcccatt ttatggatta ctgtcctcat tctactgagt atcatcacaa
    24361 tcatttcgat agtcaaccgt aatacgactc ctgagaataa ggtaacctgg ttgttagtag
    24421 cctttgtgcc agtatttggt cccttgctct atctgatgtt tggtgaaagg cgattgtcca
    24481 aaaaagaaat caaacaactg aagaagctag gctctatgca tttccaagaa gcaaatagcc
    24541 agctactaaa agagaaatta aaagaaagtg acaaggcagc ttatggagtc atcaagtcct
    24601 tattgagtat ggataccaat gctgacatct atgatcaaac tgcctctaca ttttttccta
    24661 acggagaagc tatgtggaaa aagatggtag aagatcttaa aaaggctgag aaatttattt
    24721 tcttggaata ttacattata gaagaaggtt tgatgtggaa tcgcatacta gatatactag
    24781 agcaaaaggt agctcagggt gtagaggtta agatgctcta tgatgatatc ggctgtatgg
    24841 ctactttaac aggagattat gcacatcgac ttcgtcagct gggcatcgag gcccataaat
    24901 tcaataaagt tattcctcgt ttgacagtgg cttataataa cagagatcat agaaaaatat
    24961 tgattgttga tggtcagata gcctatactg gtggggtcaa tctggcagat gagtacatta
    25021 accacgtcga gagatttggt tattggaagg atagtggaat tcgcttagac ggactagcag
    25081 taaaagctct gacacgctta tttttgacca cttggtacat taatcgagga gaaattagtg
    25141 attttgatca atatcattta gaaaatcatt ctatcccgag tgacggttta accattccat
    25201 acggaagtgg acccaagcca atttttcgag cgcaggtagg gaaaaaagtt tatcagagtt
    25261 taatcaatca agcaacagaa tcggtctata ttacgacacc ttatttgatt atagattatg
    25321 atttaacaga gacaatcaaa aatgcagcta tgagaggggt cgatgttcga attatcaccc
    25381 cttacatacc agataagaag ttcattcagt tagtcacgag aggagcttat cccgaccttc
    25441 tttctgctgg tgttcggatt tatgagtata gtccaggttt tattcatagt aagcagatgt
    25501 tggtagacga agattttgcg gtggtgggga caatcaatct cgactaccgg agcttggtac
    25561 accattatga aaatgcagtc ttactctata aaactccttc tataagggaa atcgcccgag
    25621 attttcgaaa tatatttgca gattctcagg aagtctatcc tcattctatc aaaacgagct
    25681 ggtatcaaaa gcttgtaaaa gaaatcgccc aactattcgc ccctatctta taagaaatct
    25741 agaacttagg atacaaacca gacctatttt tcttgtcttt tacgaataga aagaaagagg
    25801 aggggaattc atgctaacta aggaagaagt gaatgatatg attgagttca aacagacaca
    25861 tttacaccgc tttcgggaaa ttgaaacctt tgtgaaacta tgcaaaaaat cactcaaaca
    25921 gccatcgcaa gctgacaatc ctaggacttt ttgatatact aagacagata aattgaacag
    25981 gagaaacgat atgagtgtgt atggtagagt agaagaagtt cataaggaga atcgtgaacc
    26041 tctagaatac caaattgaac aagaatcgca tcatcgtgaa tcaagtcgtc ttcctttggt
    26101 gaaaatttta ctatggagta cgcttgtaac ggggataact ctaggagtac cgctattact
    26161 cgacttaatg agtgcacaag aagtgcagga tttttatgca ggttgggcgc ttcatcagac
    26221 agggaagatt tacagcgact attatggaag tcaaggtttg ctttattatt tgctgactta
    26281 cgtgagtcag ggcggatttt tctttgccat ctttgagtgg ttagccttgg tagcaggcgg
    26341 atttttcctt tttagatcag cggacacctt gacaaatcaa ggagaccaag ctggacaact
    26401 ggtgactatt ttttacatgc tagttacagg tcttgctttt ggtggaggct atgcgactct
    26461 tttagcgctt cctttcttat tcgcagcctt tagtttagtt gcggcttacc taagcaatcc
    26521 aagccatgat aagggatttg tacggattgg gctagctttg gcaggcggat ttttctttgc
    26581 tcccttatca tcgctcctgt ttattgctgt agtgagttta ggcttgttgg tctttaacct
    26641 tgggcataga cgctttgcgc atgggtttta tcagtttctt gcagtggctt taggtttttc
    26701 acttgtcttt tatccaactg cctactatag tgctgcaaca ggaagttttg gggatgcgat
    26761 tagtggtatt cgttatccta ttgacagtat tcgctttgat tttacttcta aaattttaga
    26821 gaatatgttt ttttatggct tgttgtccct tggtttggga tttgtggttt gtatcttttt
    26881 aggtctcttt caatccaaac cctttaaact atacgtcatt tcggttcctg caagtcttgt
    26941 ggtaatttta ggtttgattt tacttttctt ttcacaagag cctctgcacg cttcttattt
    27001 gatggtcgtc ttccctgttt tcctactttt attggtaacc aatattaaga gtcaacagag
    27061 ggggcgtagt gctagaagaa gccgaagaga aacgccagtt agcctatgga gtcgtttctt
    27121 caaaggaaat ctatatctgc tagtttttgg gtttgtctat cttttgtctg ttcctttttt
    27181 gatgaagttt gtcctttatc cagtacctta tcaagaacgt aatcgtcttg ctgatttggt
    27241 aaaagaggag acaaatacgg aagatgctat ctatgcatgg gatgatactg cgactcttta
    27301 tcgtaagagt gagcgcttgt cgccatcggc gattttgtcc ccgttgcact atacagcaac
    27361 tgaggaaaat cgtaataagt tacttaatga cttgaaagaa aaacaaccta aggtgattgt
    27421 ggtaaatgac aaggtggaag tctggtctga agtggaaaca ctcttaaaag aaaattacca
    27481 acaagtaaag actgattact cagagtttaa agtctataaa attaaataac caaatcaata
    27541 tcttgtgtat ttttaaaaat tttaagattt ttaacacaag atattgattt ttctttttag
    27601 agtggtataa tactttttag aaagaacatt ttagaaaaga gcatgcatat gattgcacta
    27661 gaagaaaaaa ttacaatttt gccaactctc ttcgtcgaga aacgagatgg gagacgtgtt
    27721 gtatttgatg tggacaagat tgacaaggct ctccacaagg cagctgacaa ggttatggat
    27781 gtgacacccc tggttgaaaa acgcctcaat gacctgactg agagaattat tacagaaatt
    27841 catagtcgct ttccacaggg aattaagatt tacgaaattc aaaatatcgt agaacatgaa
    27901 ctccttgaag ccaaagaata tgcgctggct gaggagtata ttacttatcg gacacagagg
    27961 gattttgagc gctcaaaagc gacggatatc aactttagta ttcataaact tctcaacaaa
    28021 gaccagacag ttgtcaatga aaatgctaat aaagacagtg atgtctttaa cactcagcgt
    28081 gatttgacag cagggattgt tgggaaatca atcggactgc aaatgcttcc taagcacgta
    28141 gccaatgccc accaaaaggg ggatatccac tatcacgatt tggactacag tccctatacc
    28201 cctatgacca actgctgttt gattgatttt aagggtatgt tggaaaatgg ttttaagatt
    28261 ggaaatgcag aggtagagag tcccaagtct atccagactg cgacagcaca gatttctcaa
    28321 atcattgcca acgttgcttc tagccagtac ggtggctgtt cagctgaccg tatcgatgaa
    28381 attttggcgc cttatgcaga gaagaactat caaaaacatc tcaaagatgc agaagagtgg
    28441 gtattgcctg aaaaacggga agattacgct tggaagaaaa cgcaaaagga catctacgat
    28501 gccatgcaat ctcttgagta tgaaatcaat actctcttca cttcaaatgg acaaacacct
    28561 tttacttcgc taggttttgg tctgggaacc agtcgttttg aacgagaaat tcaaaaagct
    28621 attttaaaca ttcgcatcaa gggtcttggt tcagaacacc gtacggctat ctttcctaaa
    28681 cttattttca ctctgaaaag aggccttaat ttagaagaag gaactcccaa ctacgacatc
    28741 aagcagttgg ctcttgagtg tgcaaccaaa cggatgtatc cagatgtttt atcttacgat
    28801 aagattgttg acttgacagg atccttcaag gtgcctatgg gttgccgttc tttcctccaa
    28861 ggatggaagg atgaaaatgg tgtagaagtc aattcaggtc gcatgaatct gggtgttgtg
    28921 acggttaatc tgcctcgtat tgctcttgag tctgaaggtg atatgaataa gttctgggaa
    28981 atcttcaacg agcgaatgaa tatcgcagaa gatgctcttg tttaccgtgt cgaacgcact
    29041 aaagaggcga caccagcgaa tgctcctatc ctttatcagt acggtgcttt tggccatcgt
    29101 ctaggtaaag aagaaagtgt tgaccagctc tttaagaatc gtcgtgcgac cgtttcgctg
    29161 ggctatatcg gcttgtatga agtagcgaca gttttctttg gtaacagctg ggaaagtaat
    29221 ccagatgcta aggaattcac gctagacatc attcacgata tgaaacgccg tgtagaagag
    29281 tggtcagacc aatatggcta ccatttctct atctactcaa caccatccga aagtctgaca
    29341 gaccgtttct gccgactaga tatagacaag tttggctcta ttcctgatat cacagacaag
    29401 gaatactaca ccaactcttt ccactacgat gttcgtaaaa atccaacacc gtttgaaaaa
    29461 ttggactttg agaaagtcta tccagaagca ggtgcgtcag gtggtttcat ccattattgt
    29521 gagtatccag tccttcagca aaatccaaag gccttggaag ctgtctggga ttatgcttat
    29581 gaccgtgtag gctatctagg caccaatact ccgattgacc gttgctacaa gtgtgacttt
    29641 gaaggggatt ttgaaccaac tgagagaggg tttgcttgtc caaactgtgg caatagcgac
    29701 cctaaaacag
//
LOCUS       DAVAAL010000013        29267 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVAAL010000013 DAVAAL010000000
VERSION     DAVAAL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.013"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..1964)
                     /gene="alaS"
                     /locus_tag="VQM60_000442"
     CDS             complement(<1..1964)
                     /gene="alaS"
                     /locus_tag="VQM60_000442"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET1080338.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVTQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTTSLIFAETPFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGK"
     gene            complement(1986..2471)
                     /locus_tag="VQM60_000443"
     CDS             complement(1986..2471)
                     /locus_tag="VQM60_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET1080339.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(2746..2985)
                     /locus_tag="VQM60_000444"
     CDS             complement(2746..2985)
                     /locus_tag="VQM60_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080340.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILSHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(3230..4300)
                     /locus_tag="VQM60_000445"
     CDS             complement(3230..4300)
                     /locus_tag="VQM60_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET1080341.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(4297..5070)
                     /locus_tag="VQM60_000446"
     CDS             complement(4297..5070)
                     /locus_tag="VQM60_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1080342.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(5067..5873)
                     /locus_tag="VQM60_000447"
     CDS             complement(5067..5873)
                     /locus_tag="VQM60_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1080343.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(5854..7011)
                     /locus_tag="VQM60_000448"
     CDS             complement(5854..7011)
                     /locus_tag="VQM60_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080344.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(7070..8020)
                     /gene="murB"
                     /locus_tag="VQM60_000449"
     CDS             complement(7070..8020)
                     /gene="murB"
                     /locus_tag="VQM60_000449"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET1080345.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(8132..8884)
                     /locus_tag="VQM60_000450"
     CDS             complement(8132..8884)
                     /locus_tag="VQM60_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HET1080346.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLVYIIYGLAMAAFFEETARLVFFKWLEKKI
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLTAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLIAYGTKEIFCKKS"
     gene            complement(8894..9625)
                     /gene="budA"
                     /locus_tag="VQM60_000451"
     CDS             complement(8894..9625)
                     /gene="budA"
                     /locus_tag="VQM60_000451"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET1080347.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGTGEQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELEERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     VGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            9767..11038
                     /locus_tag="VQM60_000452"
     CDS             9767..11038
                     /locus_tag="VQM60_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET1080348.1"
                     /translation="MKRQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFMAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(11058..11873)
                     /locus_tag="VQM60_000453"
     CDS             complement(11058..11873)
                     /locus_tag="VQM60_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET1080349.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(12030..12683)
                     /gene="phoU"
                     /locus_tag="VQM60_000454"
     CDS             complement(12030..12683)
                     /gene="phoU"
                     /locus_tag="VQM60_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET1080350.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(12695..13453)
                     /gene="pstB"
                     /locus_tag="VQM60_000455"
     CDS             complement(12695..13453)
                     /gene="pstB"
                     /locus_tag="VQM60_000455"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET1080351.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(13466..14269)
                     /gene="pstB"
                     /locus_tag="VQM60_000456"
     CDS             complement(13466..14269)
                     /gene="pstB"
                     /locus_tag="VQM60_000456"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET1080352.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(14280..15164)
                     /gene="pstA"
                     /locus_tag="VQM60_000457"
     CDS             complement(14280..15164)
                     /gene="pstA"
                     /locus_tag="VQM60_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET1080353.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(15154..16071)
                     /gene="pstC"
                     /locus_tag="VQM60_000458"
     CDS             complement(15154..16071)
                     /gene="pstC"
                     /locus_tag="VQM60_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET1080354.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(16071..16949)
                     /gene="pstS"
                     /locus_tag="VQM60_000459"
     CDS             complement(16071..16949)
                     /gene="pstS"
                     /locus_tag="VQM60_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET1080355.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(17523..18827)
                     /locus_tag="VQM60_000460"
     CDS             complement(17523..18827)
                     /locus_tag="VQM60_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET1080356.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCVSLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(18828..19601)
                     /locus_tag="VQM60_000461"
     CDS             complement(18828..19601)
                     /locus_tag="VQM60_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET1080357.1"
                     /translation="MESKFEFAKGLVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGHLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(19591..19869)
                     /locus_tag="VQM60_000462"
     CDS             complement(19591..19869)
                     /locus_tag="VQM60_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET1080358.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(19869..20270)
                     /locus_tag="VQM60_000463"
     CDS             complement(19869..20270)
                     /locus_tag="VQM60_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET1080359.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(20480..21097)
                     /locus_tag="VQM60_000464"
     CDS             complement(20480..21097)
                     /locus_tag="VQM60_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET1080360.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETVQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(21087..21860)
                     /locus_tag="VQM60_000465"
     CDS             complement(21087..21860)
                     /locus_tag="VQM60_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET1080361.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     TGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(21871..22608)
                     /locus_tag="VQM60_000466"
     CDS             complement(21871..22608)
                     /locus_tag="VQM60_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET1080362.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(22613..23743)
                     /locus_tag="VQM60_000467"
     CDS             complement(22613..23743)
                     /locus_tag="VQM60_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET1080363.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(23827..24216)
                     /locus_tag="VQM60_000468"
     CDS             complement(23827..24216)
                     /locus_tag="VQM60_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET1080364.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(24232..24615)
                     /locus_tag="VQM60_000469"
     CDS             complement(24232..24615)
                     /locus_tag="VQM60_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET1080365.1"
                     /translation="MLEVAYILVALAVIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(24616..25404)
                     /locus_tag="VQM60_000470"
     CDS             complement(24616..25404)
                     /locus_tag="VQM60_000470"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET1080366.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(25397..26332)
                     /locus_tag="VQM60_000471"
     CDS             complement(25397..26332)
                     /locus_tag="VQM60_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET1080367.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(26462..26638)
                     /locus_tag="VQM60_000472"
     CDS             complement(26462..26638)
                     /locus_tag="VQM60_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET1080368.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(26778..27485)
                     /locus_tag="VQM60_000473"
     CDS             complement(26778..27485)
                     /locus_tag="VQM60_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET1080369.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            27636..28664
                     /gene="queA"
                     /locus_tag="VQM60_000474"
     CDS             27636..28664
                     /gene="queA"
                     /locus_tag="VQM60_000474"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET1080370.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
BASE COUNT         8654 a         6733 c         5577 g         8303 t
ORIGIN      
        1 tccttgccgt atttctcacc aaagagggcc atagctccca tttctttagc agtgtcaata
       61 tccgtttcaa ctgtcttaac ttcaagagct tcccagattt tttcgttgac ttgctgttca
      121 atcgcacgca attcttcagc agttacagct tggaagtggg taaagtcaaa gcgaaggaat
      181 tcaacttcgt taagagatcc tgcctgtgtt gcgtggtttc caaggatatt gtgaagggca
      241 gcgtgaagca aatgagtcgc agtgtggttt ttcatgacac ggtgacggcg attgctatca
      301 attgccaagg tatattcttg gttcaaggca agcggtgcaa ggacttcaac tgtatgaagg
      361 gcttgaccat ttggggcttt ctgaacattg gtcacagtag ccacaacctt acctgactca
      421 tccaagattt gtccgtggtc agcgacctgt ccacccattt cagcatagaa tggcgtttcc
      481 gcaaagataa gtgaggtagt tccttctgaa acagcttcta cttctgcatt gtccgccacg
      541 atagctacca atttagaaga caattggcta gcattgtagt tgaagacact ttctacagtg
      601 atgttttgaa gagtttcatt ttgcataccc attgagccac ccttgacagc tgaggcacgc
      661 gcgcgttctt gctgttcttt catggctgct tcaaaacctt cacggtctac agtcatacca
      721 gcttcttcag cgatttcttc agtcaattca actgggaacc cataagtatc atagagtttg
      781 aagacatctg aaccagcgat aacagattga cctttttctt tcaagtctgc tacaatgcct
      841 tgggcaaagt gttgacctga gtgaagggta cgggcaaatg attcttcttc gctcttaacg
      901 attttctcaa taaagtcacg tttctcaagc acttctgggt agtagctttc catgattttt
      961 ccaacagttg gaaccaattt gtaaaggaaa ggttcgttga tacccaattt ttgaccatgc
     1021 atagaagcac gacggagaag acgacgaaga acataaccac gaccttcatt tcctggaagg
     1081 gcaccatcac cgatagcaaa tgaaagagaa cgaatgtggt ctgcgataac cttgaagctc
     1141 atgttgtcgc catcttggtc ataaacctta ccagacaatt tctcgacttc acggataatc
     1201 ggcatgaaga ggtccgtttc aaagttggtc ttagcccctt gggtaacggc caccaaacgc
     1261 tccaaaccag cgcccgtatc aatgttctta tgtggcaatt ccttgtattc gctacgagga
     1321 acagcagggt ctgcgttaaa ttgtgacaaa acgatgttcc agatttcaat ataacggtcg
     1381 ttttcaatat cttctgcaag caggcgaaga ccgatatttt ctgggtcaaa ggcttcccca
     1441 cggtcaaaga agatttctgt atctggtcca gaaggtcccg caccgatttc ccagaagttg
     1501 tcctcaattg gaatcaagtg acttggatcc actcccactt caatccagcg gttgtaagaa
     1561 tctttatcgt ctggatagta ggtcatgtaa agtttttcag cagggaaatc aaaccattca
     1621 gggcttgtca aaagctcata agcccaagtg atagcttcgt cacggaagta atccccgata
     1681 gagaagttcc ccaacatttc aaacatggta tggtgacgcg cagtcttccc tacgttttcg
     1741 atgtcgttgg tacggatagc cttttgggca ttggtaatac gtggattctc agggataatg
     1801 gtcccgtcaa agtatttctt gagggttgct accccagagt tgatccacaa aagagttggg
     1861 tcgtttacag gaaccaaact tactgatggt tctactgagt gacctttggt cgcccagaaa
     1921 tcaagccaca tttggcgtac ttgtgcacta gatagttgtt tcatattgtc tccttattta
     1981 cttgtttaat gtgattggct ttccagcatt tccacatagt caatcgcgac acagagggaa
     2041 atgactaggt ctgcataagc gtcctcaaga accgttacgg tataggtaga ggtcagatgg
     2101 aagagttcct tcttaatttc cgcaatcagc tgatcgcgat catccagcaa tttgaaattc
     2161 aaatcccaga tattgccctc gatacgaaga cctagattat caaactcata cttatctcgc
     2221 cagaaggtca acttcttacg aatgacaaaa ctcgagccat cccgaagctg aatctcaaaa
     2281 cgaggaagca aggtcaagat ttctttacta atctcactga cttgttcacc agccgcatca
     2341 tagatggtaa aggttttagg aatcttaaaa aatgatccct ccacctgata ggcaatttct
     2401 cccctgtcat ccttgatagc gaagcgttcg cctccaagac gaaacttttg tttgacaaga
     2461 aatgttttca tcaacacctc caaaaatcaa aagacaagct catatcacga agggcgaaaa
     2521 accgcggtac caccttcatt caatgaactt gtcattctct tgttcttatg caattgtatg
     2581 attgagtagc atgacttcct agcttagatg gctcgcagca ccgccatttc tctggactaa
     2641 gacaagtgaa aatcaattct caactttctt attataacgt ttttttaagc ttgcgtcaac
     2701 tggaaatgat ctccgttgaa ttagaccaat tccctacatc tctgattact ttttcaggat
     2761 atattttttc ttactgccgt ttttcttttt atcccaaatt ttcatattac taaacacagc
     2821 tactagaata tttccaaata taaaggtgcc tatcacccaa tatatggact cagttgttag
     2881 gtattgtcga tccaagccat cctttaaatg ggatagtata acagtttggt taacaatcat
     2941 aaaggttggc cagaaacttt ttttgaaaaa agtagacatt ttcattattt gttgccgctt
     3001 tctgtaaggt taatactcaa taaaaatcaa aaagcaaact aggaagctag cctcaagctg
     3061 tacttgagta cggcaaggca acgctgacgt ggtttgaaga atataggctt agtatactac
     3121 taggcaagca aataagcaac tagaatagaa aaagataggg ttctaaaaac tgacttctat
     3181 tccttaaaaa cgaaccagct tgactgattc gtcttcttac gtttatctcc tacttccgat
     3241 acattttaaa ctgtaggaag aggtcgctat atttccctgt ccatttatgg tcaaatttct
     3301 cataaacttc taggtgtttc atggtttcaa catctggata gaaggcctta tcttcctttg
     3361 tttcctctgg gagcaattcc ttcgctggta ggtttggtgt tgaatagccg acatactccg
     3421 cattttggag agcgttttca ggtttcaaca taaagttgat aaaggcatag gctgagtctt
     3481 ggtttttaac tgttttggga atgaccatat tgtcaaacca aagattgctg gcctctgtcg
     3541 gtaccacata acgtagattt tcattttttt ctaacatttg gctggcttca ccagagaagg
     3601 tcacgccgat tgcaacatta ttctgaatca tgtagccctt catctcgtcc gcaacgatag
     3661 ccttgatatt tggagtcagt ttgtagagct tatccactgt ctcttccaac tgctgcggat
     3721 ccttggagtt gaggctgtag ccgagggaat tgagtcctag tcccagcacc tcacgcgccc
     3781 catcaaaaag catgatagaa ttcttatact ccagcttcca aaggtcatcc caatgctcag
     3841 gcgcttcatc taccatggtt tcgttgtaga caattcctaa ggttccccag aagtaaggga
     3901 tggagaattt attacctggg tcaaaggact ggttgagaaa ctctggtccg atattttcga
     3961 ttccttcaat ttttgaataa tcaagcggaa ccaagaggtc ttcgtccttc atcttgttaa
     4021 tcatgtattc acttggaatg gcaatatcgt aggtcgttcc accctgcttt atcttagtgt
     4081 acatggcttc gttggagtca aaagtctcgt actgaacttg aattcctgtt tcttctgtaa
     4141 actgagtcaa gagttcagga tcgatatagt ctccccagtt atagataacc aatttttgac
     4201 tatctcgact attgatttta ctatctaaat gagtcgcaat tccccacaag acaaggataa
     4261 tcgctgcaat tcctgctaaa aatgaataga tttttttcat gcttgctcct ccttctcacg
     4321 agagataaag taataaccta caactaggat aatactaaag agaaagacta gagcagacag
     4381 ggcattgatt tctaaggaaa tccccttgcg agcacgagag taaatctcga ctgatagggt
     4441 tgaaaagcca tttcctgtta caaagaaggt cacggcaaag tcatctaacg aataggtgaa
     4501 ggccatgaaa taaccagtaa tgatagacgg agtcaggtaa ggaagcatga tttccttgaa
     4561 catctgaaat tgactagctc ccaagtcata ggccgcatga atcatgtcgc cattcatttc
     4621 cttgagtcga ggcaagacca tcaagaccac gataggaatg gagaaggcca cgtgactaga
     4681 tagaacggtc aaaaagccaa gtgaaaactt gagttgggta aagagaatca agaagctagc
     4741 accaatcata acgtcaggcg caaccatgag gatattattg agtgatagaa aggcttcttg
     4801 gtatttctta cgagactggt agatgtaaat ggcaccaaaa gtcccgataa tggtcgctat
     4861 caaggctgat aggaaggcca agaaaaatgt ctgagccaaa atcagcatga gtctcccatc
     4921 tccaaacatg gtttcaaagt gagtccagct aaaacctgta aagctattca tatcatcacc
     4981 agcattaaag gcatagccaa tcaagtaaaa gataggcagg tagaggacca gaaagaccag
     5041 tcccagataa aggttggcaa attttttcat cgttctctcc tttccttagt cacccacatg
     5101 gtgatgaaca tggtcaggat gagaatcaca ccgatggttg aacccatacc atagttgtca
     5161 ttggttagaa aattctgctc aatagccgtc cccaaggtga taacgcggtt cccaccaatc
     5221 aaacgggtca gcatgaagag actcaaactt gggataaaga ccgactgaac cccacttcgc
     5281 acaccgttca tagatagagg gaagatgaca tgacggaagg tctcccactt agttgcacca
     5341 aggtcataac tagcattgat gagattatta tccatgtcgt ctaagacatt gaaaatcggc
     5401 aaaatcataa aggggagctc gatgtagctt gcgacaaaga taaaggaaaa atcggtaaaa
     5461 agcaactgtt gtgaaccaat tccgataaat tccaagaatt ggttaataga gccattttga
     5521 ccaaaaatcc cgataaaagc ataggcttta aggagcaaat tgatccaggt aggaaggata
     5581 atcagcatga gccagagttg acggtgcttg agacgggtca aaaagagggc tgttggatag
     5641 ctgataagca aggtcacaaa ggtcacaatc cctgcataaa gcactgagtt gaaactcatt
     5701 ttgaggtagg tcaagttttg tgacgcaaag taagatttat aattttctaa actaaactgg
     5761 ccttcaatgt tgaaaaagga ttgtccgaaa atcaagacca agggtgccaa tacaaagagg
     5821 gcaatccaaa gcatgtaggg cactacaaag agtttagagc ttgttttctt catctctttc
     5881 ctcctcgatc gcattgatca gacctgcttc ttgctcttcg atttccacgt attcttcgat
     5941 acgagcatcg aactcttctt cggtttcatt gagacgcatg atgtggatgt cttctgattc
     6001 aaagtccaga ccgatttcct cacccacgat ggccttacgg gttgagtgga tcatccattc
     6061 atttccaagt tcgtcatagg cgataatttc ataatgaact ccacggaaga gctgggtgtc
     6121 gaccttaact tggagtttgc cttcttcagg aagggtaatg cgcaagtcct ctggacgaat
     6181 aacgacctca acaggttcat ttggcttcat cccaccatca accgcttcaa agcgtttgcc
     6241 gttaaattcg accaagtagt cctcaatcat ggtacctggc aagatgtttg actccccgat
     6301 aaaggtggca acaaagtggt tgattggctc atcgtagatg tccacagggg ttccagactg
     6361 gacaatctcg ccatcattca taacgaaaat ccagtcactc atggcaagag cttcttcctg
     6421 atcgtgagtg acaaagacaa aggtaatgcc caatcgttgt tgtaattcac gcaattcgta
     6481 ctgcatgtct gttctcaatt tcaagtccag cgctgataaa ggctcgtcca acaagaccac
     6541 acggggttgg ttgatgatag cacgggcgat ggccacacgc tgacgttgtc ctccagaaag
     6601 tttgcggatg gaacgttttt cataaccttc caactgaacc atcttgagaa cttccgctac
     6661 acgctgctcg atttctttct tatcaatttt acgcaagcga agtggaaagg caacattttc
     6721 aaacacattc atatgtggga acaaggcata ggattggaag acggtatgta cgtcgcgctt
     6781 gttggttgga atatcattga tacgaacacc gtctagcatg atatctcctg tcgtcgcatc
     6841 cagtaaacct gcaataatgt ttaggatagt tgatttcccc gaaccagatg cacctagaag
     6901 ggtgtagaat ttcccttctt ccaactcaaa gttgatgtct ttgagaacct tggtgttgct
     6961 gtcttcaaaa actttagaga cgtttttgaa ttcgataatt ggttttttca attggcataa
     7021 attccttctt tttcatagat taaccgatcg gggctctgtc aggtccccac tacctcttgc
     7081 agggagtaaa accacctgca tacatcttcg ctaccgatag gctttcaccc aagatccgga
     7141 cttctctttc aagcgtaata cctgagtgtt ccttgacttt ttcgataacc gattggatca
     7201 agtcctcgta gtctttggcc gttccatctg cgacattgat cataaatcct gcatgctttt
     7261 ctgacacttc tacgccaccg atacgatagc ctttcaagcc agcttctgaa attaactgac
     7321 ctgcaaaatg cccgactgga cgcttaaaga ccgagccaca agatgggtat tccaaaggtt
     7381 gcttgagttc acgtaggtgc gtcaagcggt ccatttcctg cttgataacc tgatgggttc
     7441 ctggagctag ggcaaattta actgacaaga caactgcacc agactcctga atagctgaat
     7501 ggcggtaacc aaaagccaag tctttagcag acagggtttc gatttctcca tccttggtca
     7561 agaccttaca agactgcaag atgtgagcaa tctcgccacc ataggcaccc gcattcataa
     7621 agacagcacc gccaacgctt cctggaatac cacaagcaaa ctcaaagcca gttaaactat
     7681 gacggagggc aatgcgagtt gtttcaatca agttagcccc agcttctgct tcaatggtat
     7741 agccatcaac agaaacgtta ttgagcttgt cacacaagat gacaaatcca cgaatcccac
     7801 catcacgaac gatgatattg cttgcattgc caagaaccat ccaagggata ttttcttggt
     7861 tggcaaattt cacaacgcga gccaactcaa aacgatttcg tggaaagacc aaataatcag
     7921 cctctccacc tacttttgta taactatagc tatgcaaggg ttccttaaaa cggatatcaa
     7981 ttccttctaa gatttcaagc attttttctc ttacagacat gtcactcttc cttttacaaa
     8041 attcattcca ttataccatt tttagagaca tttgacgacc ataaaaatac cttgtttgga
     8101 ttttgcataa gaaaaagagg ttcccccctt tttatgattt tttacaaaag atttccttgg
     8161 ttccataggc gatcagaacg agctccagtg ctagaatcac ttcaaccaag actggatttg
     8221 tcaaccagcc tacttggaaa agagatggtg ccagatcaaa gaaggcatgc aagccatagg
     8281 ctgttaggag ataaatccat ttcttctggc gaacagcttg ataaacccaa actgtcaaaa
     8341 gtaattggaa accaagcgcc aagattcgct caaaaccaag caaataaatc tgccagaccg
     8401 aaagtgactg aatggttttt aacatatttt cagacagtaa ttgcataacc tgtggattct
     8461 gagtttgaac tgccgaaaga acaatgtaaa gattgagtaa actagtaagg cctagaaaaa
     8521 tcaactccaa gccaccatgc cccagtccat aggccaaggc gtctgccttt tccaaactta
     8581 tctttttctc caaccatttg aagaaaacaa gacgagccgt ttcctcaaaa aatgctgcca
     8641 tggctaggcc atagataata tagacaagcg ggtgatcttg caagagggca atactaccgt
     8701 ctttttgagg atgcaaaatc aagatatgca ccagtttttc caaaatctgc gaagagacaa
     8761 agaaagcgac tgcacccaag cccaagacag ctagattaat ctggtatttc tttttggcaa
     8821 accaaatgct tcctatcaga aaagctagca acagcaacat ggtaataatg atatgaatgg
     8881 tcattttctt ctcctattct gccttttcaa tatctttttt catctcgtca acattgaact
     8941 tagcaaacaa gtattgacgg tcttggactg ggaaacgttg gtccaactgg tcaactgctc
     9001 ccacctcgat aatgccttcc ttgatgacaa aatccatgac atgtccaccg aaggtcaaat
     9061 catctgatat gaagtgcaga tggtagcctg ccacactgac cccatggaaa atctcaggcg
     9121 tccagaaacc aacaatagtc cccacaacat tgtcacgact atattccggt tgatgggttg
     9181 cgacatcagc aaacttggta tcgggtgttg acttaggaat catacgcaca tgcatatgtg
     9241 aaaattctcc ccgaatcttg atagagcgga aaagattttc cccatcataa taagactcaa
     9301 ttcgttcttc caattccttg tctgtcatct caaagcgctg gcggaaaatg acctctgcct
     9361 gatgcggtac tactgctgca taaggaataa gggcatctgg tgacacttcc acaatttctg
     9421 gctgttctcc tgttcctttt gcctgataag ccttgccatc taagacaatc aattccccat
     9481 caatagaatc caaggttccc aaaccaaggt caccatgctc tagcaattct cccactgtca
     9541 tggtaccacc ataaaggcca gccatcaagg ctccaagagt attgtattga aataatttca
     9601 ctggttcctg cacgttctta tccattcact ttcttgtctg ttattctata aatcttactc
     9661 ctaagtatac cacatttgcc cctagatgtg aacgagagaa acgctctaga cattgccaag
     9721 aaggaaaaaa aagggtacaa tgtaacaaaa tcaagggagg tctggaatga agagacaaag
     9781 caagtacaaa gaggtcgttt cctatctgaa aaatggtatc gagtctggac gatttccgac
     9841 gggtagtcgc ctgccttcta tccgtcaact gagccttgac tttcactgca gcaaggacac
     9901 cattcaacga gccctgctgg aattacggca cgaacaatac ctctatgcca agcctcagag
     9961 tggctactat gtattagaac aagggcaaca tcaagaccta gaaatcgagg ttaccgacga
    10021 acatgccagt gcctatgacg atttccgact ctgtgtcaat gaaaccttga ttggccgaga
    10081 aaactacctc ttcaactact atgacaatca agaaggatta gaagacctaa gacagtccat
    10141 tcacaaactc ctctttgagc aagctctcta ctgcaaggct aaccaactag tactgacttc
    10201 tggaacccaa caagccttgt ttatcctctc tcaaatatcc tttcctagac aagccaagga
    10261 aatcttggtg gaacagccaa cctaccatcg gatgaatcgc ctcttgattg cacaggggct
    10321 ggactatcaa acgattgaac gaggcattga tgggattgac ttggaggagc tggaaggcca
    10381 cttcaaaaca ggaaaaatta aatttttcta caccattccc cgatttcact atcctctggg
    10441 acattcctat tctgagcaag acaaacgatc tattcttaac ttagctgcca agtatgatgt
    10501 ctatatcgta gaggacgatt atctgggtga tttggactcc aagaagggcc aaaccttcca
    10561 ctatcttgat acagaggagc gtgtcattta tatcaagtcc ttctcgacca gcctttttcc
    10621 tgcccttcgt attacagcac tcattcttcc aaatgctatc aaagaagcat ttatggccta
    10681 caaaaatatc ctagactacg acagcaacct cattatgcaa aaggccctgt cactctatat
    10741 tgacagtcaa ttgtttgaaa aaaatcgttt ggctcgcttg accaatcatg aatcttacca
    10801 aaaacaaatc gaggaaagga taactaaaac accttgtccc cttcctcatt atcccctaca
    10861 cgatggttta ttgctagacc tgagacagta tcctaaaatc gccagtctca aacacagtca
    10921 actgggcttg gacttctttg aagaggccta tttaagcacc tgtccttatc aatttgccaa
    10981 ggtgtcccta gacaatctgg aaaatgtttt aaactattta aaagcagaat tggaatgaca
    11041 tccaactctg ctttttctta ttcttcaact tcagttagac ttgctgcttt ttcaagatag
    11101 ctttggtaag ttccttcatc cttaagtttt tggatgacct tgtctaccac ttctttcaaa
    11161 tcatcactat tttttctaag tgcaacggca ttggcgtcgc cgtccttcat cttcaagctg
    11221 acagttgcga cagctaagcc agcgttttta gcagcataac taagtgcaac aggctcatcc
    11281 atatgaacag catctatttt tccagcctgc aattcattga ctgcttcacc catattagtt
    11341 agggaagtta attgagcttt tggcaattgt tccttgacca ttgattctgg aacagtccct
    11401 ttttgggctg caatattagc actttctagg ctagttaaat ccttgtattt ttccacatca
    11461 gccttacgaa ccaagaaact aatcttgttt tcatagtatg ggattgaaaa atcaaagact
    11521 tcttttctct cgtcagtagc actaattcct gcaactgcta ggtcagcctt accagtttga
    11581 agactggtca aaacattgtc aaaactcatg cttaagattt ccaacttaac cccaagttca
    11641 tcagcgatag cttgagccat gtcgatgtct gcaccgacta cctggttctt tccatcaacc
    11701 aatgattgaa attcaaaggg tgcatagtca ggactggttg ccacaactaa tttccccttt
    11761 tgcttaatgg cctcaacagc tgactgagaa ccattagaac ctgactggca agctactaag
    11821 aagaaacttg caagaaaatt acataaaaca aatatccatt tttttgattt cataaataaa
    11881 caacctttct gttaattttg tataattata acaatatcca agttacttgt caagtgtttt
    11941 ttaaattttt atctcaaaaa tattttttcg ttcaaaaaaa ggagccatca gttgatttca
    12001 agctcctttt tatacagaat taaactattt tatagttcga caatcttacc tgtttcaaag
    12061 tagacaaccc attcacagat atttttagca tagtcaccga tacgctccaa gtaggaaata
    12121 acttggaaat aatcacgacc cgtaacaatg gcttctggat ttttcttaat ctcttcagtc
    12181 gcaaggtcac ggatagtttc aaaatagtgg ttaatttgct catccatgga ggccacccgg
    12241 tatgcgtcgt caacagaacc attaagataa agatcaagtg ctgcttcaac aacgctttta
    12301 acttcacgtc ccattttttt aatttcttcc tctacagctg ggatgcgttc ttcccccttc
    12361 atacggatgg ttgcctgggc aatggctaca gcgtgatccc ccatacgctc cacatctgat
    12421 acagccttaa ggacagtcaa gactgtacgc aaatcttgag agactggttg ttggagtgcg
    12481 atcatttcaa atgatttctt ttccagtttc acttcgtatt catttacttc tgcatcatct
    12541 tctatgacct cttttgccag gtcacggtca tgcgtgacaa aagcacgtac cgtacgattg
    12601 atttgtgaga gcacttcttg tcccatagcg tagaactggt tatgtaattt ctctaaatct
    12661 tcttcaaatt gagatcgtaa catctttcat ctccttatcc aaattttcct gtaatatagt
    12721 cttccgtttc cttgtgttgg ggatcaagga acatctgctt ggtatcatta aattcaatca
    12781 aatctccatc taggaaaaat cctgtcttat cagagatacg tgaagcttgc tgcatggaac
    12841 gggttaccag aagcatggtg tacttgtctt ttagaccata caaggtttcc tcaattttac
    12901 cagctgaaat cggatccaaa gccgaagttg gctcatccaa gaggatgatt ttaggactag
    12961 ttgccaagac acgggccacg cagacacgct gctgttgacc acctgacaat ccaatagctg
    13021 aatcatatag acgatccttg acctcatccc agatagaggc accttgcaag gctttttcta
    13081 cggcttcatc cagaacctgc ttatccttaa ttccattgat acgaagcccg tagacaacat
    13141 tctcatagat agtcataggg aaaggattag gttgttggaa aaccattccg atttccttac
    13201 gtaattcaac cgtatctgta cgcggactgt agatgttgtg accattgtac accacggatc
    13261 cagttgtggt cacctctgga ttgagatctc ccatgcggtt gagagacttg aggagggttg
    13321 acttccctga tccagatgga ccaatcaagg ctgtaatttc cttaggttgg aaagataggg
    13381 aaacactatt caaagccttc tttttattat aataaacgga caggtctgat acctgtaaaa
    13441 tcgcatctgt catacggttt cctttctaac caaagtgacc agatacatag tcattggtgg
    13501 actgtagctt ggcattttgg aaaatagttg cagtcttgtc atactcaatc aaatcaccca
    13561 agtaaaagaa gcctgtatag tcacttgcac gagcagcctg ctgcatatta tgcgttacaa
    13621 tgatgatggt aaagtttttc ttgagctcaa acatggtctc ttctagttgc atggtcgcaa
    13681 tcggatccaa ggctgaggct ggctcatcca ttaagaggat atctggctta acagagatgg
    13741 cacgagcgat acagagacgt tgttgctgac cacctgataa ggtcaaggct gacttgtgga
    13801 gatcgtcttt aacctgatcc cagagggcag cctgacgaag ggaggtttct acgatttcat
    13861 ctaggacttg cttatcctta actccagcac gttcatgcgc aaaggtaata ttacggtaaa
    13921 ttgacttagc aaatggattg gggcgttgaa aaaccattcc aatgtgttta cgcatttcat
    13981 aaacgttgat ttctggacgg ttgacatcaa ttccacgata gagaatctgc ccagttactt
    14041 tagcaatatc aatagtatca ttcatgcgat tgagactgcg taagtaggta gatttccccg
    14101 accccgacgg accaatcaaa gctgtaattt tatttctttc aaattgcata tcaatcccct
    14161 taatggattc atttttacca tagtaaacat ggacatcctt agtagaaagg gctacttttt
    14221 cttcaggaaa ggtaaggata tgcttctcat cccagttata tgttgacatg gcttctcctt
    14281 taggcagcgg ttaatttctt gtgtagatag cttccgaact tacgagctcc aaagttaaaa
    14341 atcaggataa agatcaggag cacagcggca gaacctgctg atacaatggt tccatctgga
    14401 atagtgcctt cactattgac tttccagata tggacagcca aggtttctgc ttgacggaag
    14461 atagagatgg ggctagtcac actgaggata ttccagttag accagtcaag agctggcgcc
    14521 gattgccctg ctgtatagat cagagctgca gcttcgccaa agatacgacc agatgccaag
    14581 acgacacccg ttacaatacc tggaagcgct tctggaataa caacatgaac cactgtctcc
    14641 cagcgagaaa tcccaagagc cagaccagcc tcacgttggg tatggtgaac gtgtttcaaa
    14701 ctatcctcta cattacgcgt catctgaggc aaattaaaga ctgtcaaggc caaggcacct
    14761 gaaatgattg aaaatccata ctcaaactgg actacaaaga tcaagtaacc aaagagaccc
    14821 accaccactg atggtaaaga ggacaagatt tcaatacaag tccgcacaaa gttggtaaca
    14881 ggaccttttt tagcatattc agccaagtag atcccagctc ccatagaaag aggtacagaa
    14941 ataatcaagg taatgaccaa taggaaaaag gaattgtaaa gctgaatgcc aatcccacca
    15001 cctgcttgaa aagcagaaga ccttccagtc aagaaagacc aagagatatg gggcaagccc
    15061 cgaaccaaga tatagagaat caaggaagcc aagattgtca caatgatgct agcaatcgta
    15121 tagaggacag ctgttgcaag tttatctaat ttcttagcgc gcataatttt tctttcctct
    15181 ttctttcgta atcaatttaa tcacactgtt aaaaactaaa ctcatcaaga gcagtaccaa
    15241 ggccagtgac cagagaacat tattatttac agttcccatg acagtgttcc caattcccat
    15301 agttaatata gaagttaaag ttgcagctgg tgtggtcaag gaagttggga taacagctga
    15361 gtttccgaca accatctgga tagctaaagc ctcaccaaag gcacgcgcca tcccaaagac
    15421 cactgcagtg aaaataccag aacgggccgc cttcaagatc acacgccaga tagtctgcca
    15481 gcgagtggct cccatagcga aactggcttc acgataataa cgaggaaccg cacgcaagct
    15541 atccgttgtc ataaaggtta cggtcggcaa aatcatgaca aagaggacgg aaatccctga
    15601 caaaatccca aaaccagtcc caccaaagac actgcgaaca aagggaacga cgacttgcaa
    15661 gccaataaat ccgtacacta ctgaaggaat cccaaccagg agttcaatag ctggttgcaa
    15721 aatcttcgcc ccttttggtg atacttcggt cataaaaact gctgcaccaa tagcaaaggg
    15781 ggttgcgata agggctgaga gaatggtaac gataaaggaa cccaaaatca taggaagggc
    15841 accaaattct ttactagaag gattccaagt tcctcccaaa agaaagtcaa agatattcac
    15901 accattgaca aagaaggtcg acaagccttt ttgcgctacg aaaaccaaaa tcatggccac
    15961 aaggatgact atcaaagaaa gacaggcaaa ggtcaaacct tttcctaatt tctccagacg
    16021 agaattcttt gatggaagca acattttctt agctaattct tcttgattca ttattgtctc
    16081 ccttccaaca ctgtcacagt tccggcagca tctttttcaa ccttcatttc cttaatcgga
    16141 atatacttca atcctttgac aatcccttct tgggtctcat ccgagagaac aaaattgaga
    16201 aattctgcag ccaactcatt gggctgcccc aatgtataca tatgctcata agaccacaag
    16261 ggccaattat tgctacttat attttctgga cttaagtcat agccattcaa cttcatgctt
    16321 ttgaccgaat catctatata ggtaagagat aaataagaga tagctcctgg actttttgat
    16381 acgattgatt ttaccgctcc atttgaatcc tgctcctgac tttgcatggc agactgacct
    16441 tccataatga cagtatcaaa ggtagcacga gaaccagagc cggctgcccg attgataaca
    16501 gagatgggta agtccttacc accaacctct ttccaattgg ttacctcacc tatgaagatt
    16561 tgacgaagtt gctctgtcgt taggttatca acatcaacct ccttattgac aatcagagcc
    16621 aagccagcta ccgcgacctt gtggtcaaca agagcagaag catcaattcc gtctttttcc
    16681 tcagcaaata catctgagtt tcctatatca actgccccag actgaacctg ggacaagcct
    16741 gtaccagaac ctcccccttg gacattgacc gtttttccaa catggatcgt gccaaattca
    16801 tctgccgcta cttcaaccaa gggttgcaag gcagttgagc caacagccgt tatggattct
    16861 ccacgatcaa tccagctagc acagcctact aaacaagccg tcagccaaaa agcgataaga
    16921 gacagagcaa gcttttttct ttttttcact gtttttctcc tcgaaaataa ttatgaatac
    16981 tgtgaatttt ttaagtagtt ctttatgagt tgacgcatga attcttacca aatttctgcg
    17041 caattgatta tttatataat ataggctata ttactctttc ctaacctcct tttttcatat
    17101 gtggataaaa tctcttgtct atcccttccc ccattgtcac ccattatagt catttcgtgt
    17161 ctctttttcc cctttttaat gcaagggaaa ttactctcct tagatgataa tccaaaagct
    17221 agaaaggtat ctcaaacctc tctactctcc cagactagtt tacaactaaa aggaaaagat
    17281 tcgattttat gagaaatcta gtttacaagc ggtaagaacg ctaataacta aacttcttgt
    17341 actctttgaa aatctcttca aaccagtgtt ttgagctatc tatggctagc ttcctagttt
    17401 gctctttgat tttcattgag tagtaaaact acatgtaatg gcaatcaaga tatcaagaat
    17461 catcctacta aaaaaatcca tactttcact ataacataga ataagatatt tgactagcat
    17521 tttcatttga atctgaggcc ttttggaaaa taatttttca aaacatttcc agtaaccttt
    17581 gcaaagccca agccattgcc tttaaccaaa acttggtacc aaccatttgg cagactttct
    17641 gccagctgaa cgatttctcc agccgcatac ttgacaaact cttcttggcc aatttcaacc
    17701 gactgttcga cctgactcgg tttcaaggct aaaccaagag cgaaactggg ctcaaagcgt
    17761 ttcttcttaa aagtacccag atgcagtcca ttgcgagcaa tcttgagctt ccctaaatct
    17821 ggcaaaagtt ctggcaagag ataaagctgg tctccaaaaa tctgcaagat acccggtaga
    17881 ttgaccttca aatggttttg ggcaaattcc tgccacaagg caacttgttc acggctgagg
    17941 ttactcttac ttgccttaaa tttaggagct ggattgttac ccttaaactg tagatgggca
    18001 acaaactgac cctctccctt aaactgatga gggtacatcc gagccgtttc tggcaggtca
    18061 ataccagcta ccattccatt gatatgctct actggcaaca agtcaaaatc atactcttcc
    18121 agcaaccaat tgacaatctc ttcgttttcc tcgggtgccc aggtacaggt cgaataaacc
    18181 agacgaccac cttcagctaa catggtcact gcatcctcca gaatttctct ttgcaagcta
    18241 acacattgac tcggataatc taagctccaa tagtccatag catcaggttg cttacgaaac
    18301 attccttcac cagagcaagg ggcatcaaga acgattaagt caaaatagcc tttaaagacc
    18361 ttgaccaagc ggtcggcaga ttcattggtc accacgacat ttgtcgctcc aaaacgctcc
    18421 atgttttcaa ccaaaatctt agcccgtttg cttgaaattt cattggaaac aagtagcccc
    18481 tcccctgcta gataggctgc cagttgagtt gatttgcccc ccggtgcagc agccaagtcc
    18541 aagaccttca taccaggact gggttgggct acttgagcca ccatttgagc agcaggttct
    18601 tgcgaataaa ctaaacctgt agcatgctca ggcgatttcc ctgaaacctt cccatagtgg
    18661 ccccaagggg tttgagtaat ggcatcagaa aaggaaagtt gctcttcttt taagggattg
    18721 acccgaaagg ccgaaaccgc ttcctcctca aaagaggcaa gaaaatctct tgcctcatct
    18781 cctagtattt ctttatattt ttcaacaaat ccttctggaa atttcattta agttcttttc
    18841 ctttcgtaaa tataggactg aatttcctcc tgcatctcaa gaggcaccat catgaccggc
    18901 tgtctggttt gaaaatcagg agcttcacca aaaagggtca caacccgata gcccagactt
    18961 tcccctaaaa tactagctgc ggcataatcc catggttgca gataggtgag ataggtcaac
    19021 aaatgccctg acaaaatctt ggcaaaacta atggccgcac ttccatagac acgaacacca
    19081 agaaccgctc ggctcaaatc agccagcccc cattcattgg tttccagcat accactattc
    19141 cctgcaatga gaaaatctcc aagtggttta gttttaaaag gagctagggg cctatcattt
    19201 agacaaactg gaaattcccc accaccgtgg taacaatccc ctttgaccac atcataaatc
    19261 agaccaaact gtccctgacc attttcaaaa taagccatca taacagcaaa atcttcctgc
    19321 tgggctacaa aattattggt accatcaatg ggatcaatga cccaaacctt gccctcttga
    19381 accgaggctc gcagacaacc ttcttcagca caaatcttat cctcaggata acgagacaaa
    19441 atctcaccaa ccaagagttc ctgaacttct ttgtccagtc tggtcaccaa atctgttgga
    19501 gaggacttgg tttcaacacg caagtcttcc tgcatatggt caagaatgta ctggcctgct
    19561 ttcttaacaa gccctttagc aaattcaaat ttactttcca agagaaatct ttccttcccc
    19621 tttttctttg gcagcctgaa ctgctcggta aagtgaatag ccactaaccg tttcaaattc
    19681 tcgtcccaaa cgtttctctt cgctcttgct tggcacgacc gctttgaatc ccttatagga
    19741 gtccagtaac tttttagcct ctactttgcc ttcataggca gcttcaacat cattaaaaaa
    19801 agaaagcact gaagcaagtt cttcagtgct ccacgacaaa tctagtgggt aactatactg
    19861 tttgttcatt aactaatacc agctctcatt cttgcttctt ttagttcttg cttacgataa
    19921 ctacgaggga gaaaagcacg aatctcatct tcattaaaac cgatttgcat acgcttggca
    19981 tcaataataa ttggacgacg caaaagacta ggatactgct caatcaaatg aagcaattcc
    20041 gataccgaaa tactctctac atcaatattc aatttttgaa aaatttttga acgagttgaa
    20101 atgatgtcat cagtaccatt ttcggtcaag gaaaggatgt gttgcaattc ttttcttgtt
    20161 aaaggactgg tcataatatt gtgttccaca aagggaacct tatgtttttc taaccaggcc
    20221 ttagccttac gacatgatgt acagctcggt gatagaaata gtgtaatcat gcttttctct
    20281 tcttatctat actttgctac ttctattata caaaaaaata aggcgcttga ctagggattt
    20341 ttagaaaaaa agcctatttt ttcaagaaaa ataggctttt tgcgaacgat tgacacaatt
    20401 ggatttggtt aattcactct taacgatggt tttaaacgat atatatattt atatatgtaa
    20461 attaaaaaca tctttccttt cacttcctac gacttttcag atacagatag ccaaagaagt
    20521 tttcatagag ggcaaaaaag aggaggaagg cgtgaagaaa gaaggtctct ggcaaaatca
    20581 taataacagg atccttggct ggatcaaaaa gccaggtatc atctcccaca aagagaattt
    20641 gatggaaaag agtaaagaat tggtcaaaac caatcaaaac tcccccaagt ccaatcatca
    20701 caggtaagac tactagagcc aggagacttt ttcgataaag agacaaaaag tcctttttca
    20761 caatcctatt gacaaagaca tagaaacttg gcagtgtcac tagagctact agctgaacca
    20821 aatgaaagag attcttgacc actgcgaaat ggtgcagacc agctgctgac gaacgaaaat
    20881 caggcatctg taagacctga ctaaaaggat tggtcagata attcatcaag atatgaaaat
    20941 tgtattgaac ggtttctggt tttagataga ctcgattcgt taagtttagc cactgaatct
    21001 ccataggata gaaaatccaa gccagataaa tggtcagaag gattgagagg gagaggagaa
    21061 agagcataga gccccaaaag gtcaatttag ttttcatcaa aatcccactc cgcaaggcta
    21121 gaaaccacat gtgtcggtgc gattggcagg ccagctactt cttctgcctt agtaaaacct
    21181 gtcgtcacca agagcgttgg aatgccattg tcaatcccag cccggatatc agtcaaataa
    21241 ttgtccccaa ccatgattaa ctcttcacgt tccaaaccta agtgctcaac cgccttgtcc
    21301 ataatgatgg catttggttt tccgatataa accggcttca ctcgtgtcgc tacttcaagc
    21361 agcgtaatca gtgagccagt acctggcaaa agaccacgtt ccgtcgggat gttgaggtca
    21421 ggattggttc cgataaaatg ggcacccttt tgaatagcaa gagttgctgt ggcaaatttt
    21481 tcatagtcga cttgccaatc cagaccaact accacgtagg cagggttttc cttgtcttcc
    21541 acataaccag ccgccttgat ggcttccttg agtcctgctt ctccgacgac atagacagtc
    21601 ttttcaagcc ccaaatcatt catatagtcg atggttgcca aagtcgctgt gtagacagtc
    21661 gatagaggcg tatcgatatt aaaattctga gccaacatct ccttaacact ctctggagtg
    21721 cgggttgtat tgttggttac aaagagatag ggaatgtccc gcttttgcaa ttcatgaaca
    21781 aaagtctctc cagcagggat tcggtctttc cccttataaa tggttccgtc taaatcaatt
    21841 aaatagcctt tatatttcat ctatttctcc ctaaaccttt tttatttctt gccaagtaat
    21901 gattgcttgg gcattgataa ccccatcact tgtaatttca tgcttgcttt ccagtccagt
    21961 ccgttcaaca gccgatgtaa tcaccccacc tggtcgaact tccttgacat acttgaggtt
    22021 gattttcttg ggaatatagt gggtcaaaaa atccgctccc atgacctcaa aaatccagtc
    22081 caagtattta ctgttattga catgaccatt catatccaag tcgtaaaaac gaacatggta
    22141 atccttgctg atcggctctt ccaaggattc atacttcggt ccacggataa gttttttatc
    22201 aaaatcagac tggtaaggag ccacaatctc aggttcaaca gcatggactt ttcgactgtc
    22261 gcggtccatg agaacaaagg tcgccatcat atggatgagc tcctgccccg cttcattata
    22321 aatagtaaag cgacggtagc aaaaaagtcg attgtagctc aaggcttccg tttcgatggt
    22381 aatttcttcc gcaaaacgag gcaaacgaac cacctcaata tcatattctg cgataatcca
    22441 gaccagatta tagtcttcca aaatggcctt atcactaact cccagttcaa tcgattgcat
    22501 ccctgaaact tgcagtgaca gcaaaatcac atctggaagt ttgatatgac cgttcatatc
    22561 agccatatca aaaggaattt tcattttcat ttgataagtt aagcccatga tcctactcca
    22621 aaataaatcg ttctgctaca gtatctccca aaaagagacc tctctttgtc atgcgaacgt
    22681 ggtcaccctc aatctgcatg aggccttgtt gaaccaaatc tctgacaatt tctccataaa
    22741 gtccagcaaa agactgtcca aatttttcct caaatcgcgc catggaaacc ccggatttct
    22801 tgcggagtcc caagaacatt tcttcttcca tttgctcctt ttgactcagg tgatcttctg
    22861 taatacaagc attgccttcc tcaaccgcac tgagataatg acgaatggga ccatgatttt
    22921 tatagcgtac tccattgaca taaccagatg cccctgcacc aataccatag tattcagcat
    22981 tgtcccagta catgagatta tgacgacttt caaagccggg tttggagaaa ttagaaatct
    23041 cataatgctc aaaacccgct cgctccagct ctgcaatgat gtactcaaac atctccgctt
    23101 ctagttcctc cttaggcaga ggcaatttcc cacgtcgcat ccggttcata aagaccgtat
    23161 ggttttctaa aatcaaacta tacaaactca tgtggggaat atccaatcca atggctttag
    23221 ccacattttc ctttacttgc tccatggtct gaccaggcag agcataaatc aaatcaatgg
    23281 agatgttgtc aaaaccagcc agtttcaggc gatcgatatt ttcataaata tccttctcca
    23341 aatgactgcg cccaatcttt ttcaacatct tatcatcaaa ggtctggaca cctagcgaaa
    23401 cacgattgac agccgaattt ttcaaaacag ctatcttatc cgcatccaaa tcgcctggat
    23461 tggcttcaat ggtcaactct tccaagacag acaaatccaa gtttttagtc aagccattca
    23521 gtaacacctc cagttgcgga gccgacaggg ctgtcggtgt tccaccaccg atataaaggg
    23581 ttgacaactt ttcaatatca taagaacgaa actcttccag cagatgctct aaatagctgt
    23641 cgactggctg atttttgatg aagacctttg aaaaatcaca ataataacaa atctgggtac
    23701 aaaatgggat gtgcacatag gctgacgttg gttttttctg catagtaatt attataccac
    23761 aaagactaga ttccagataa aaatcaccat ccccagatac atagtccgtc cggagatggt
    23821 gatggtttat tcttctgtta tatcaatcac aatctcttct gagtcatcaa gagcttcggc
    23881 tttttcttgc cattgttcct tgagattatt taattgattt tttgatgctt ctgtcgcttg
    23941 aaaagcatag gatttagctt gagcaagtat actgtccaca gtgatttcac ctgactcaac
    24001 ctgttctttt gttttcagaa caaaatctgt agcctgctcc ttaacttctg tcagtttttc
    24061 acagacttgc tccttggcat actccggatc ttctctcaaa tcatctagaa aatcttgagc
    24121 ctgactgcaa acttgtttgc ccttatcact tgttaaaaac aaggcaagag ctgcacctga
    24181 aacggttcct aaaaggattg aggataattt acccataagg attctccttt tttatttttt
    24241 gaaaaattta cttgcaagac gaagagctga cagacttgca ccagtcttga gtgtttttga
    24301 accagctgat gaagctttct tgctcaagac acgcgcatgg tcattgaggt ctgaaacaga
    24361 tagagataaa tctgcaacag cactgaagag tggatcaatc gtagccacct tgacattgat
    24421 atcatctgcc aagacattga ccttagccaa caactcattg gtgtgatgca aggtcacatc
    24481 cacatctgaa gtcaaggttt taatcgtctt ttctgtttca tcgatgacac gaccaagctt
    24541 ttgtacagta atgatcagat agaccaaaaa gacaatcaca gctagggcaa caagaatata
    24601 tgcaacttct aacatttagt tttcctcctc tgtaatatag taaggggcct tctttcgatt
    24661 ttgataaata acgatcatta taccgagacc gataaagaca actgacagcc attgggacac
    24721 tcgaaggccg aagaacatga gactatctgt tcgcatacct tcgatgacca tacgaccgaa
    24781 accataccaa atcaagtaaa aggccgtgat atgacctcgt ctgagactct tccatttccg
    24841 tctaaaaatc agaatcaagg caaagccaag cagattccat agagactcat aaaggaaagt
    24901 cggttgacgg tagctcccct caatatacat ctggtcacgg ataaagccag gtagataatc
    24961 cagattatcc actgttgcac cataagcttc ttggttaaag aaattacccc aacgccccaa
    25021 actttgagca atcataacgc taggcgccgc aatatctaga aaatcccaag tattgatgag
    25081 tttacggtca gcaaagatat agagcacaag agccccagtt atcaaaccac cgtaaatggc
    25141 caaaccacca ttccaaatgg caaaaatctc tcctaaattc tgactatagt aatcaaatcg
    25201 gaaaataaca tagtagagac gagctcctaa aatagccaag ggaaaggcta ctaagataaa
    25261 atctaaaata tcgtctggta tgatcttctt tctaggtgct tctttcatgg tcaaataaac
    25321 cgcaagaatc aagcctgtca caatacataa ggcataccaa cgaatggcta ggggtcctag
    25381 ttgaatagca attggatcaa gcattttgca cctcatttcg agctatgaga cttgtcagtc
    25441 gttcgtcgaa caaacgggta gcatcaaagc ccatttcctt ggcacgataa ttcatggcag
    25501 ctgcctcaat cacaacagag atattacgac ctgttttaac tggaatacga atacgaggaa
    25561 tggctacgcc agaaacttca agttcctctg cattgtttcc aagacgatca aaggtcttat
    25621 gcgtatcgta attttccaaa tagacagcaa gctgaacctg tgaagaatcc ttgacagcac
    25681 tcgcaccgta gagactcata acatcgataa taccaacccc acgaatttca atcaagtgtt
    25741 tcaaaatttc agctggttca ccccagagag taatctcatc cttggcaaag atatcgacac
    25801 ggtcatcggc taccaaacgg tgaccacgtt tgacaagctc aagacctgtc tcgctcttac
    25861 caattccact atctccctga atcaagacgc ccatcccata aatatccatc aagacaccgt
    25921 gcacactggt acgttctgcc aaacgagaat ccagatagct agataactct ccagataaac
    25981 gactggtagc tgcacggctg gttaaaatag caatcttaca ttctctagca gcctttaaca
    26041 tctcctctgg aaccaccaaa ccacgggcaa caatgactgc tggtgtctca ggtagaaaca
    26101 tttttttcaa aacttcataa cggctgttgg aaggcatgct gatcagataa gaccactcct
    26161 tcatccccaa aagttgaatc cgctctggtg tatagtagtc aaaatagcct gtcatttcaa
    26221 gaccaggtcg cgtaatatcc gctgtattga tttccttttc aagcaattct ggttcaccat
    26281 agacaatatc tagtctaagc ttttcaatca cttcttttac taaaaccgac atcatttcct
    26341 ccttcaaatg aattcttctc tctattatat caaattgtag gaggaagttt cagatttttg
    26401 caggctttgg agtatttatt taaaagaaaa aggactagat ttctctagcc tttcatttca
    26461 attagaattt tttacgttta cgagctactt ctgatttacg tttacgtttt acagaaggtt
    26521 tttcatagaa ttcacgtttg cgtgtttctt gaagagtacc agctttagta accgcacgtt
    26581 tgaaacggcg aagtgcgtcg tcaagagatt cattcttacg tactactgtt ttagacattt
    26641 ttttcactcc cttcaagttc aatatctatt tcattctaca cgtaaaagga ataattgtca
    26701 agcaaaaatg cggtttaatt ttgatttttt cttccattta tacaagagtt gaaagcgttc
    26761 aatagaacat tgctttttta tttttcgagt aagctaagcg cttcagcatc tgcgatgatg
    26821 gttacatcag ggtgattttg gaggctactc gcaggtaggt tctcagtcac tgggccagat
    26881 actgttccgg caatggcttc tgctttcgac tcaccataag caaaaagaat aatagacttg
    26941 gcatccaaaa tgtttttaat ccccattgaa atagcttggg ttgggacgtc ttcaatcttg
    27001 gcaaagaagc gagcattggc ttcgatagta gactggtcaa gttctactag atgcgtttga
    27061 ctgtcaaatg gagtaccagg ctcattaaag ccgatatgcc cattgcgacc gattcccaag
    27121 atttggaggt caactggatg gtcagccaaa atttggttgt agcgttcaac ttcagcttca
    27181 gcattgtcct taacaccacg aggcaagaaa ctttctttaa atggtttttg gttgaacaag
    27241 ttttcttgca tgaagtaacg ataagactgt ggattgtccc catcaaggcc cacatactca
    27301 tcaaggttga cactggttag atttgaaaaa tcaaggtcac tctcaacaat ttccttgtaa
    27361 aattcaagtg ggctacttcc tgtcgcaagt cctagagttt gagctccatt agccaatttt
    27421 tccttcaaaa tctcaaaagc aacttttcca ccttgaactt ggttttcaac tttaataact
    27481 ttcatctttt catcctccat ttctgtctat atttattata tggtatagac caatttttgt
    27541 caagtgaaaa cgatttaatt tctaaaaaaa gagcgtccaa acttgaaacg tgttataatg
    27601 gataggatta gaacttagaa agaatgaaca gatatatgaa tacagctgat tttgatttcc
    27661 acttgccaga ggaattgatt gcccaaacgc cccttgaaaa acgtgatgcc tccaaactcc
    27721 tcatcgtcaa ccgtgagaca ggagaaatgc aagataaaca cttccactct attattgata
    27781 tgctggaacc tggtgatgcc cttgtcatga acgacacccg agttctccct gcccgcctct
    27841 atggtcaaaa agtggagaca ggaggtcatg tggaacttct cctccttaag aacactagtg
    27901 gagacgagtg ggaagttctg gctaaacctg ccaaacgcct caaggtcggt actcgtatca
    27961 gctttggtga tggccgcctc agcgctgtcg ttacagaaga attgacccac gggggacgca
    28021 ttgtccgctt tgaataccaa ggaattttcc tagaagtctt ggaaagtctg ggagaaatgc
    28081 ctctgccacc ttatatccac gaaaaattag atgaccgtga acgttatcaa accgtctacg
    28141 ccaaggaaag cggctctgct gcagcaccga ctgctggtct tcacttcacc aaagaactgc
    28201 tggcagaaat ccaagctaag ggtgttcatc tagtctatct gactctccat gtcggactcg
    28261 gaacctttag acctgtttct gtggataatc tggacgaaca cgaaatgcac tcagagttct
    28321 atcaactttc tgaggaagct gctgccaccc ttcgctctgt caaaaaaaat ggtggtcgtg
    28381 tcatcgctgt cggaaccact tctatccgca ccttggaaac tattggttcc aagtttgatg
    28441 ggcaaatcca agcagattct ggttggacca atatctttat caaacctggg tatgagtgga
    28501 aggtcgtgga tgccttctca accaacttcc acctgccaaa atcaactctg gtcatgttgg
    28561 tttctgcctt tgcaggccgt gaattagtct tagatgccta ccaccattcc atccaagaac
    28621 actaccgctt cttcagtttt ggtgacgcca tgtttattta ttgagaaaga atttctctaa
    28681 atcttctaat accaataaat cgctaagata ttatttcaaa gaacatctac aattgaaact
    28741 ctagctagct gtagaagagg cctagtacat tgaaattaaa atgcttcccc ctagcttcga
    28801 aaatattgcc atagattgcg ttgactctcc aaattgattc atctatattt tatttcagct
    28861 tcctatattt tcttcgctgt ttgtaaatca aaatgcaaga cacatgagta gcaccatatt
    28921 tgttactctt atctgtcctc tcaagagact attatgagtt atttcagaat cattcactac
    28981 tttgaccctg actctcctta gtctcaaaat caaagactta tactcttcaa aaatctcttc
    29041 aaaccgcgtc aacgtcacct tggattatat atgtgactga cttcgtcagt tctatctaca
    29101 acctcaaagc agtactttga gcaacctgcg actagttttc tagtttgctc tttgattttc
    29161 attgagtatt aaacaaaaag tgaacaaatc tgaattctaa tgtacagaag actaggcttg
    29221 ttcacttttt tatagtcgct ataagatgac cttatctata gcttttt
//
LOCUS       DAVAAL010000014        28711 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVAAL010000014 DAVAAL010000000
VERSION     DAVAAL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28711
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.014"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            520..1797
                     /gene="ltrA"
                     /locus_tag="VQM60_000475"
                     /pseudo
     CDS             520..1797
                     /gene="ltrA"
                     /locus_tag="VQM60_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            2257..3435
                     /locus_tag="VQM60_000476"
     CDS             2257..3435
                     /locus_tag="VQM60_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET1080371.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTCINLVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            3749..3880
                     /locus_tag="VQM60_000477"
     CDS             3749..3880
                     /locus_tag="VQM60_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET1080372.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            3901..5451
                     /gene="dltA"
                     /locus_tag="VQM60_000478"
     CDS             3901..5451
                     /gene="dltA"
                     /locus_tag="VQM60_000478"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET1080373.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            5448..6692
                     /gene="dltB"
                     /locus_tag="VQM60_000479"
     CDS             5448..6692
                     /gene="dltB"
                     /locus_tag="VQM60_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET1080374.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            6706..6945
                     /gene="dltC"
                     /locus_tag="VQM60_000480"
     CDS             6706..6945
                     /gene="dltC"
                     /locus_tag="VQM60_000480"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET1080375.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            6938..8206
                     /gene="dltD"
                     /locus_tag="VQM60_000481"
     CDS             6938..8206
                     /gene="dltD"
                     /locus_tag="VQM60_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET1080376.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            8361..8801
                     /gene="adcR"
                     /locus_tag="VQM60_000482"
     CDS             8361..8801
                     /gene="adcR"
                     /locus_tag="VQM60_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET1080377.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            8801..9505
                     /locus_tag="VQM60_000483"
     CDS             8801..9505
                     /locus_tag="VQM60_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET1080378.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            9498..10304
                     /locus_tag="VQM60_000484"
     CDS             9498..10304
                     /locus_tag="VQM60_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET1080379.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            10314..11819
                     /gene="adcA"
                     /locus_tag="VQM60_000485"
     CDS             10314..11819
                     /gene="adcA"
                     /locus_tag="VQM60_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET1080380.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYTEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(12990..13763)
                     /locus_tag="VQM60_000486"
     CDS             complement(12990..13763)
                     /locus_tag="VQM60_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET1080381.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            13939..15342
                     /locus_tag="VQM60_000487"
     CDS             13939..15342
                     /locus_tag="VQM60_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET1080382.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            15457..16095
                     /locus_tag="VQM60_000488"
     CDS             15457..16095
                     /locus_tag="VQM60_000488"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET1080383.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            16107..16547
                     /locus_tag="VQM60_000489"
     CDS             16107..16547
                     /locus_tag="VQM60_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HET1080384.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            16540..16971
                     /locus_tag="VQM60_000490"
     CDS             16540..16971
                     /locus_tag="VQM60_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET1080385.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            16962..17432
                     /locus_tag="VQM60_000491"
     CDS             16962..17432
                     /locus_tag="VQM60_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET1080386.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            17457..18230
                     /locus_tag="VQM60_000492"
     CDS             17457..18230
                     /locus_tag="VQM60_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET1080387.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            18227..19027
                     /locus_tag="VQM60_000493"
     CDS             18227..19027
                     /locus_tag="VQM60_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET1080388.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            19086..21380
                     /locus_tag="VQM60_000494"
     CDS             19086..21380
                     /locus_tag="VQM60_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET1080389.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFDPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTNYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            21390..24506
                     /locus_tag="VQM60_000495"
     CDS             21390..24506
                     /locus_tag="VQM60_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET1080390.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            24563..26329
                     /locus_tag="VQM60_000496"
     CDS             24563..26329
                     /locus_tag="VQM60_000496"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET1080391.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            26475..27626
                     /locus_tag="VQM60_000497"
     CDS             26475..27626
                     /locus_tag="VQM60_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET1080392.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGYDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            27771..28655
                     /locus_tag="VQM60_000498"
     CDS             27771..28655
                     /locus_tag="VQM60_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET1080393.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
BASE COUNT         9019 a         4740 c         6141 g         8811 t
ORIGIN      
        1 atagcggata aggaggcttc aaactctaaa gtccaaaaag gtagtcgtaa cctatatgcg
       61 taaatcacga gagtaattga attcggacta aggtttgtgt gaaaagataa atctttctag
      121 agtctaaaga ctctgcgtca gatttcctat tttcactgta accttttaac gtcctcatat
      181 cttgtataaa cgaggaaaga tgtacgactt atcccgtgag gtctcatgag tgctgaaagc
      241 gtagtaacaa cgaatcatga gaagtcagcc gaacccatag tagtgaggaa acttccgtaa
      301 tggaagtgga gcgaaggggt gaatactcaa acagtctggg gagagactgt ttgaggtctg
      361 tcgctagaaa gagaaaacga cagattgaag taatcctact tcacttgtgt ctgtaaaacg
      421 agaggtctga tagaactgga gtctgtcacg tattgactag atgaaggctc accaatataa
      481 gatgtccctc aggcaccaaa acaagaaagg aatacgcaca tgtcaaaatt gctagataag
      541 atattatcac gcgaaaatat gctggaagcc tacaatcaag taaaatccaa taaaggctca
      601 gctgggattg atggaatgac tatcgaagag atggataatt atctcagaca aaactggcgc
      661 ttgactaagg aactgataaa acagagaaaa tataagcctc aaccagttct tagagttgag
      721 atacctaaac cagacggagg tatccgtcaa ctaggaattc caacagttat ggatagaatg
      781 attcaacagg ccattgtcta agtcatgagc cccatttgtg aaccccattt ctcagatacg
      841 agttatggtt tcagaccaaa taggtcatgt gaaaaagcca tcatgaagct cttagaatac
      901 ttaaatgacg gctatgagtg gatagtggat atagacctag agaaattttt cgatacagtt
      961 cctcaagata gattgatgtc cttagtacat aacattatcg aagacggaga tacggaatcc
     1021 ttgattcgta agtatcttca ttcaggtgtt atcattaatg gtcaacgtta taaaacacta
     1081 gttggtacac cacagggagg aaatttatct cctctcttat ccaatatcat gcttaatgaa
     1141 ttggacaagg aattagaaaa gaggggactt cgatttgtgc gctacgcaga tgattgtgtg
     1201 attacggtcg taagcgaggc agccgctaag cgtatgatgt attcagtcag tcgttttatt
     1261 gaaaaacggc taggtttgaa agtaaacatg accaagacta agattaccag accaagagag
     1321 ttgaaatatc taggttttgg gttctggaaa tcatcagatg gttggaaaag tcgtccacat
     1381 caagatagtg ttcggagatt taagcttaaa ttgaagaaac taacacagag gaaatggagt
     1441 atagacctaa caagacgtat tgagcaactg aatttgtcta ttcgaggatg gataaactat
     1501 ttctcattgg gaaatatgaa aagtatagtc gccagcatag atgagcgctt gcgtactcgc
     1561 ctacgagtga ttatctggaa gcaatggaag aagaaatcga gacgattatg gggattgctt
     1621 aagttaggag ttcctaaatg gatagcagat aaggtatctg gctggggcga ccattatcaa
     1681 ttagtagctc agaagtcggt acttaaacgt gctatatcaa aaccagtcct ggaaaaacgt
     1741 ggactggttt cgtgtttgga ttattacctt gaacgacatg cgttaaaagt tagttgaacc
     1801 gccgtatgcc gaacggcacg tacggtggtg tgagaggggc tagagattat cccctactcg
     1861 attaactccc ctgaaattta ttttaattat gcaaatttca cgtatttttg atgctgagac
     1921 gacgatcctg ggaacttttc agatattttt ttgactatct aaatctatca ttagaaaagc
     1981 ttagagcgcc aaaggatttg agcgtttttc tgatttttaa gactttttcc agtctctttt
     2041 tcgattgaag atgtaattat tctactaact aactaactaa cttcttagta ctagccaaca
     2101 acgataatca taattcctcc taaaattagg aataataaag gcaatagttt ttgttttttc
     2161 atgtaaaaaa cctcactttt gttttctgct attttatgct aaaatattaa aaatcaaatt
     2221 taattccaaa gtttgtaact aaagggggag cgctacatgt ctaattcatt tgtcaagttg
     2281 ttagtctctc aattatttgc aaatttagca gatattttct ttagagtaac aatcattgct
     2341 aacatataca ttatttcaaa atcagtaatt gccacatcac tagttcctat cttaatagga
     2401 atatcctctt ttgttgcgag tcttttagtt ccgttggtta ctaaaaggtt agcgctaaat
     2461 agggttttat ctttatctca atttggaaag actatattat tggcgatact ggtaggaatg
     2521 tttaccgtaa tgcaatccgt agcgcctttg gtgacctatc tatttgttgt tgcaatttcc
     2581 atactagatg gttttgcagc acccgtttcc tatgctattg tgccacgcta tgcgaccgat
     2641 ttgggtaagg ctaattcagc cttatcaatg actggtgaag ctgttcaatt gataggttgg
     2701 ggattaggtg gactcttgtt tgcaacaatt ggtctgttac ctaccacgtg tatcaattta
     2761 gtcttgtata tcatttctag ctttctgatg ttatttcttc ctaacgctga agtggaggtg
     2821 ttagagtcag aaactaatct tgaaattttg ctcaaaggtt ggaagttagt tgctagaaat
     2881 cctagattaa gactttttgt atcagcaaat ttattggaaa ttttttcaaa tacgatttgg
     2941 gtttcttcca ttatacttgt ttttgtaacg gagttattaa ataaaacgga aagttactgg
     3001 ggatattcta atacagcata ctctattggt attataatta gtggcttaat tgcttttagg
     3061 ctatctgaaa agttccttgc tgctaaatgg gaaagtattc tttttcctct tgtagctatg
     3121 gcaatagtga cactgactat tctatatttt ccaaacgcac agatgttttt attattttca
     3181 gctttagttg gtatgttatc gcaactaaaa gaagttcctg aaagtgtatt tcttcaggaa
     3241 acagtagagg aaaatcattt agttaatgtc tattccgttc ttgaagtgat ttctacatta
     3301 gcattttcag tatttgtttt gctaatgagc tatattactg agagttttgg tattagcatc
     3361 agtttttggc tatcagccat ttgtctgatg atagaagcta ttttaattta tatcagacga
     3421 gattatttta aatgagagag tggttaaaaa gtctttaaaa atcagaaaag cgcatagtat
     3481 caggtgttga aaaaacttga tattgtgtgt tttattatgg gaagatttac ttcattgtct
     3541 cctgaaatgg agttttgaag caggatcact ctatttgaaa atatacatag aaaaactgta
     3601 taatccttaa aataagctga aatctattgg aaacatacgg taaacaattg aaaagatact
     3661 atataaagtg gtacaatggt agaaaaataa gctagtaaga atcactctta ttttagcgaa
     3721 aaattacaga aatgaatggt ttttcttgat gaaacagaga aaagaattgt acctctttct
     3781 tggtcggaca gccttgtatt ttcttatctt tctagggctg ctttacttct ttagctatct
     3841 tggtcagggt caaggaagct ttatctataa tgaattttaa cttgaaggag aatccctatt
     3901 gtgtcaaata aaccaatagc agatatgatt gaaaccattg agcattttgc tcagacacag
     3961 cctagctatc ctgtctataa tgttttgggg caggaacaca cttatggcga tttaaaggct
     4021 gattcggata gtttggctgc agtcattgac caactaggct tgcctgagaa gtctcctgtg
     4081 gttgtttttg gtggccaaga atatgaaatg ttggcaacct ttgtagcgct gactaagtca
     4141 ggtcatgcct acattccaat tgatagccat tcggccttgg agcgagtttc agctatttta
     4201 gaagtagcag agccaagctt gattattgcc atttcagcct ttcccttgga gcaggtttct
     4261 acaccaatga taaatctagc tcaggttcaa gaagcctttg cccaagggaa caactatgaa
     4321 atcacgcatc cagtcaaggg agatgataat tactacatta tctttacttc tggtacgact
     4381 ggtaagccta agggagtgca gatttcacat gataatctcc tcagctttac aaactggatg
     4441 attacggata aggaatttgc gacaccgagt cgtccgcaaa tgctggcaca gccaccttat
     4501 tcttttgact tgtctgtcat gtattgggca ccgaccttgg cacttggtgg tacgcttttc
     4561 actcttcctt cagtcatcac tcaggacttt aagcaactct ttgcggctat cttttcattg
     4621 ccaatcgcta tctggacatc aacaccatcc tttgcagata tggccatgtt gtctgaatac
     4681 ttcaacagtg agaaaatgcc tggaatcacg catttctact ttgatggtga agaattgacg
     4741 gtcaaaacag ctcaaaaact gcgcgagcgt ttcccaaatg cccgtatcat caatgcttac
     4801 ggcccaacag aagcgacagt agctctgtca gcagttgccg tgacagacga gatgttagcg
     4861 actctcaaac gcctaccaat cggctatacc aaggctgatt ctccaacctt tatcattgac
     4921 gaggaaggaa ataaactgcc aaatggcgaa cagggagaaa tcattgtttc tgggccagct
     4981 gtttcaaaag gttatatgaa aaatcctgaa aaaacagcag aagccttctt tgagtttgaa
     5041 ggtctgccag cctatcacac aggcgatgtg ggaaccatga cagatgaggg cttgcttctc
     5101 tacggcggac gcatggactt ccagattaag tttaacggtt accgcattga gttagaagat
     5161 gtctctcaaa acctcaacaa gtctcgcttt atcgaatctg ctgtcgcagt accgcgctat
     5221 aacaaggacc acaaggtaca aaatctattg gcttatgtca tcttaaaaga cggtgttcgt
     5281 gagcagtttg agcgagatat cgatattacc aaggccatca aggaagacct gacagacatc
     5341 atgatgtcct atatgatgcc atctaaattc ctttaccgag acagtttgcc actaactcca
     5401 aatggaaaga ttgacatcaa aggattgatt aacgaggtga ataagagatg atggagtttt
     5461 ttcaacagct tcctcattta gagccatatg gcaatcctca gtattttgtt tatgtgattg
     5521 ctgcaacctt gcccatcttt ataggtctct ttttcaagaa acgctttgcc tggtatgaag
     5581 tgttggtaag tctcttcttt attgtcacca tgttggtggg tggaaagacc aatcaactag
     5641 ctgccttggg tatttacctt tgctgggaaa tattgctcct gcttttctac aagcattatc
     5701 gaaaaagcaa ggatggcaag tgggtcttct acttagttag ttttctgtcc ctacttccga
     5761 ttatctttgt caaggtgcaa ccagctatca atggaacgca gtctttgctt gggttcttgg
     5821 gaatttctta ccttaccttt cgttcggttg ggattatcat cgagctgaga gatggagtga
     5881 ttaaggattt taccctctgg gaattcctcc gtttccttct cttcatgcca actttctcga
     5941 gtggtccaat cgatcgcttt aagcgattta atgaaaatta tcaggctatt cctgagcgag
     6001 atgagttgat ggatatgctg gatgaatctg tccgctatat catgcggggc tttttgtata
     6061 agtttatcct agctcatgtt ttaggagaga ccttactacc tcctctgaag aatttagcct
     6121 tgcagtcagg tggcttcttt aatctctatg ccttggcagt tatgtatact tttggtctgg
     6181 agctcttctt tgactttgca ggttattcta tgtttgcttt ggccatctca aacttgatgg
     6241 gaatccgtag ccctatcaac tttaacaagc cctttttatc aagggattta aaggagtttt
     6301 ggaatcgctg gcatatgagt ctgtccttct ggttccgtga ctttgtcttt atgcgaatgg
     6361 tgatggtgtt aaccagaaag aaagtcttta aaaatcgtaa tgtaacctca agcatggcct
     6421 acattgtaaa tatgctgatt atgggatttt ggcatggtgt gacctggtac tatatcgcct
     6481 atggactctt tcatggacta ggcttggtca tcaatgatgc ctgggttcgc aagaaaaaaa
     6541 cgctcaataa ggaacggaaa aaagcaggga aggctgccct acctgagaat cgctggattc
     6601 agttgcttgg catggttgtc actttccatg ttgtcatgtt gtcattctta atcttttctg
     6661 gattcttgaa taatctatgg tttaaaaaat aaaaggaaat aaaatatgga tatcaaatca
     6721 gaagttattg aaattattga tgagttgttt atggaagatg tttctgacat gatggatgaa
     6781 gatctttttg atgcaggtgt cttggatagt atgggaacgg ttgagttgat tgtggagatt
     6841 gagaaccgtt ttgacattcg tgtccctgta acagagtttg gtcgtgacga ctggaataca
     6901 gctaataaaa tcatagctgg tattgtggag ctacaaaatg cttaaacgct tatggatgat
     6961 cttcggaccg gtcttgatcg ctggtttgtt ggtttttctg ctcattttct tttatcctac
     7021 tgagatgcat cataatctag gagctgaaaa gcgttcagca gtggctacta ctatcgatag
     7081 ttttaaggag cgaagtcaaa aagtcagagc actatctgat ccaaatgtgc gttttgttcc
     7141 cttctttggc tctagtgaat ggcttcgttt tgatggtgct catcctgcgg tattagctga
     7201 gaaatacaat cgttcctacc gtccttatct tttaggacag gggggagctg catcgcttaa
     7261 ccaatatttt ggaatgcaac agatgttacc acagctggag aataaacaag ttgtgtatgt
     7321 tatctcacct cagtggttca gtaaaaatgg ctatgatcca gcagccttcc agcagtattt
     7381 taatggagac cagttgacta gttttctgaa acatcaatct ggggatcagg ctagtcaata
     7441 tgcagcgact cgcttactgc aacagttccc aaacgtagct atgaaggacc tggttcaaaa
     7501 gttggcaagt aaaggagaat tgtcgacagc agacaatgaa atgattgaat tattggctcg
     7561 ttttaatgaa cgccaagcct ccttttttgg tcagttttcg gttagaggct atgttaacta
     7621 cgataagcat gtagctaagt atttaaaaat cttgccagac cagttttctt atcaagcaat
     7681 agaagatgtt gtcaaagcag atgctgaaaa aaatacttcc aataatgaga tgggaatgga
     7741 aaattatttc tataatgagc agatcaagaa ggatttgaag aaattaaagg attctcagaa
     7801 aagctttacc tatctcaagt cgccagagta taatgacttg cagttggttt taacacagtt
     7861 ttctaaatct aaggtaaacc cgatttttat cattccacct gttaataaaa aatggatgga
     7921 ctatgctggt ctacgagagg atatgtacca acaaacggtg cagaagattc gctaccagtt
     7981 agaaagtcaa ggttttacca atatagcaga tttttctaag gacggcgggg agcctttctt
     8041 tatgaaggac accattcacc ttggttggtt gggttggttg gcttttgaca aggcagttga
     8101 tcctttccta tccaatccca caccagctcc gacttaccat ctgaatgagc gctttttcag
     8161 caaagattgg gcgacttatg atggagatgt caaagaattt caatagataa tataatatag
     8221 aagagttcaa gaatgaaact agttttcacg ttttttcttg actctttttt tgcttgtgat
     8281 ataattaact ggtaaacaaa atgtcaaaga atagtatttt tctcttaaaa aagagaagtc
     8341 taaaaggaaa ggagtcagat atgagacagc tagcaaagga tatcaatgct tttttgaatg
     8401 aggtgatttt gcaggcggaa aatcagcatg aaatcctaat aggtcattgc actagcgagg
     8461 tggccctgac caatactcag gagcatatcc ttatgctctt gtcagaggaa tctttaacaa
     8521 attcagaatt ggcccgtcgt ctcaatgtca gtcaggcggc agttaccaag gccattaagt
     8581 ctttggtcaa ggaagggatg ttggaaacat ctaaagattc taaagatgcg cgtgtgattt
     8641 tttatcagtt gactgacttg gctcgtccaa tcgctgagga gcaccaccat caccatgagc
     8701 atacactttt aacctatgaa caagtggcga ctcagtttac tccaaatgaa caaaaagtga
     8761 ttcagcggtt tttgactgct ttagtaggag aaatcaaata atgagatata ttacggtaga
     8821 ggatttgtcc ttctattatg ataaggagcc tgttcttgaa catatcaatt atagtgttga
     8881 tagtggggaa tttgttacct tgactgggga aaatggagcg gctaagacga cgctcatcaa
     8941 ggccagtctt ggaattctgc aaccacgcat tggaaaggtg gctatttcaa agacaaatac
     9001 gcaaggtaag aaattgagaa tagcctatct tcctcaacaa attgccagtt ttaatgctgg
     9061 ttttccaagt acggtctatg aatttgtcaa gtcgggtcgt tatccgagaa aaggctggtt
     9121 ccgtcgtttg aatgctcatg atgaggagca tatcaaggcc agtctggact cagttggcat
     9181 gtgggaacat cgagacaaac gcttgggttc tctatctggg ggacaaaagc agcgagcggt
     9241 gattgcgcgt atgtttgctt ctgaccctga tgtgtttatc ctagacgagc cgacaacggg
     9301 gatggatgca ggaagtaaaa atgaatttta cgaactcatg catcacagtg cccatcatca
     9361 tggcaaggct gttttgatga ttacccatga ccctgaagaa gttaaggatt atgcggatcg
     9421 caatattcat ctagtccgta accaagactc gccatggcgt tgtttcaatg ttcatgagaa
     9481 tggccaggag gtgggccatg cttagtttat tatcttacga ctttatacaa cgcgcctttt
     9541 tggcggttat tgctatgagt cttttctcac cggtattggg aaccttcctc atcttgcgtc
     9601 gtcagagttt gatgagtgat acccttagcc acgtctcact ttcaggtgta gcctttggtc
     9661 tggttttggg gatttctcca actgtttcta ctattgccat tgtcttgatt gcggcggtct
     9721 ttctggagta tctccgtacg gtttacaaga gctttatgga aatcgggaca gctatcctca
     9781 tgtcaacagg tctggctgtt tctctgattg tcatgagcaa gggtaaaagc tcgagttcaa
     9841 tgagtttgga ccaatatctc tttggttcga tcgtgactat cagtgaagaa caggtcattt
     9901 ccctctttgt cattgcggcg gttgttttga ttttgacctt tctctttctt cgtcctatgt
     9961 atatcttaac ttttgacgaa gatacggcct ttgtggatgg cttgccagtt cgtaccatgt
    10021 ccattctttt taacatggtg acaggggtgg ctattgccct tatgattcct gcagcaggag
    10081 ctcttctggt atcgaccatt atggtcttgc cagctagtat tgccctgcgt ctggggaaaa
    10141 actttaaatc ggttatgctg cttgccagtg cgattggctt tttgggaatg gtagcaggac
    10201 tttacatttc ctactatgca gaaacacctg caagtgcaag tattaccatt atttttgtaa
    10261 ctgtctttat actaatcagt ttagtaagac gttttatcaa ataggagacg aacgtgaaaa
    10321 aaattagctt attactagcc agtctatgtg ccttgttttt agtggcttgt tccaatcaaa
    10381 aacaggcaga tggtaaactc aatatcgtga caacctttta ccctgtctat gaatttacca
    10441 agcaagtcgc aggagatacg gctaatgtag aactcctaat cggtgctggg acagaacctc
    10501 atgaatacga accatctgcc aaggcagttg ccaaaatcca agatgcagat accttcgttt
    10561 atgaaaatga aaacatggaa acatgggtac ctaaattgct agataccttg gataagaaaa
    10621 aagtgaaaac catcaaggcg acaggcgata tgttgctctt gccaggtggc gaggaagaag
    10681 agggagacca tgaccatgga gaagaaggtc atcaccatga gtttgacccc catgtttggt
    10741 tatcaccagt tcgtgccatt aaactagtag agcacatccg cgacagcttg tcagcagatt
    10801 atcctgataa aaaagagacc tttgagaaga atgcagctgc ctatatcgaa aaattgcaag
    10861 ccttggataa ggcttacaca gaaggcttgt ctcaagcaaa acaaaagagc tttgtgactc
    10921 aacacgcagc ctttaactat cttgccttgg actatggact caaacaagtc gcaatctcag
    10981 gcctttctcc agatgcagag ccatcagctg ctcgcttggc agaattgaca gagtatgtca
    11041 agaaaaataa aatcgcctat atctattttg aagaaaatgc ctcacaagcc cttgctaaca
    11101 cactttcaaa agaagcaggt gtcaaaactg atgtcctcaa tcctttagaa agtctgacag
    11161 aagaggacac caaggctgga gaaaactaca tttccgtgat ggagaaaaac ctcaaggctt
    11221 tgaaacaaac aacagaccaa gaaggcccag caatcgaacc tgaaaaggca gaggatacca
    11281 agacagtcca aaatggttac ttcgaggatg cagctgtcaa ggaccgcacc ttgagtgact
    11341 atgcaggtaa ctggcaatca gtttatcctt tccttgaaga cggcacgttt gaccaagtct
    11401 ttgactacaa ggctaagttg actggtaaga tgacccaggc tgagtacaag gcttactata
    11461 caaaaggcta tcagacagat gtgactaaga ttaacattac tgataatact atggaatttg
    11521 ttcaaggtgg acaaagcaag aaatacactt acaagtatgt cggtaagaaa attttgactt
    11581 acaagaaagg caatcgtggc gtgcgtttcc tatttgaagc cacagatgct gacgctggac
    11641 aattcaagta tgttcagttt agtgaccaca atatcgcccc agttaaggca gaacatttcc
    11701 atatcttctt tggaggcaca agccaagaag ccctctttga agaaatggac aactggccaa
    11761 cctactaccc agataaccta tctggccaag aaatcgccca agaaatgttg gcgcattgat
    11821 gagaaatcga ctagttcata agagctagtc ggttttttga tactagatgg ctaacttcat
    11881 tatagacctt tcagaactaa gggaacctga aaaaataaat aaaactcttg aaatatctgg
    11941 gcttatatgc tagaatgtag ttagtgtata aggttcatta gaacaccaaa gcccctaact
    12001 agtgccatag ttaagggctt ttttgacgta tcttagcgat ttaacgggtg tttataggct
    12061 agtcagtcgg tctacttctt accatctagc catttgcaga cgaaatacaa gataattcca
    12121 gctataacat ctgctataat agtaagagca agctctggta taaggctcat tagtttcacc
    12181 tcctctcacg aacccatagg aacgtaatcg gtaaccgatg acaaaagtat tataccacac
    12241 taaatttaga tcatcaacct cacttaatgc ttgaactatt gtagattata tgctaaaatg
    12301 tagttaattg ataaggttca ttagaacacc aaagccccta actatggccg tagttaaggg
    12361 cttttttgac gtatcttagc gatttaacgg gtgtttatag gctagtcagt cggtctactc
    12421 cttactatct agccatctgc agacgaaata caagataatt ccagctataa catccgctat
    12481 aatagtaaga gcaagctcta tgataaggct cattagtttc acctcctctc acgaacccat
    12541 aggaacgtaa tcggtaaccg atgacaaaaa tagtatacca caatacattt agatcatcaa
    12601 ggtcgcttaa ttcttgaaat atagggtcta ggcgacaaag tctttaaaat cagaaaaacg
    12661 cataatatca ggtgtgcaaa aacttgatac tatgcgtttt attgtgggaa ggtttactcc
    12721 attttctcct gaaattgagt ttttgtccag tctctgtttt tagggttgct aagaaaataa
    12781 tgtcatgtgg tgaatatttg taaatcagtc agcagacaga acgaatactc ttcgaaaatc
    12841 ttttcacatc atgtcagctt cgtctttccg tatatatgtg actgacttca tcagttctat
    12901 ctacaacctc aaaacagtgt ttcgagctga cttcatcaat tttcaaatct gtactttgag
    12961 caagctgaga ctagcttcct atttgatttt cattgaatat cagaaaccca ttctccatca
    13021 aataattcga ctgcgtctaa taatttttgg tctggcaagg tgtcagaaat aaaggttgtg
    13081 tatttggata ggggattaat tttaaaaaat ccagtcttgt aaaatttaga actatcaatc
    13141 agtaagatgg tttcatgggc tttgtcaata atattctttt ttgaaatagc ttggctgaga
    13201 gaagcttcat aaacatattg gtcatcaata cctcttgctg aacaaaatgc taaatcgata
    13261 ttaaaatgat ctaataaaga attttcctta tcatagttga ccacggaaca ggattgatgt
    13321 ttgacctcgc cagatgtgat aaagattttg gagctatctt taacagtttc agatagggtt
    13381 tgtgcagtat gtaaaccatt tgtaaaaata atcaaattat caagttcaga aagataggga
    13441 caaagttcgt agacagtagt actagaatct agatagatac acataccaga ccgaataaag
    13501 tctttagcga gactagcgat tagtcttttt tgcctagtac tttctccttc acgtatttga
    13561 tgagaaagtt caattgtgtt catagaggac agggtcacgt atccgtgctt tcttttgata
    13621 agaccttgat tttctaagaa aattaaatca cgacgtaagg tacttgtgct ggagaaagtg
    13681 atttctgcca gctcttttac ggcaattctt tttttctttt tgataatttc aatcaattca
    13741 agtacacgtt catcttttat cataagctcc tcctaattta tcatttcaac tatattatag
    13801 cacaaattgg aggaatttga attattttta tgaatattgg gttaacattt gaacattatt
    13861 caagtaagcg ttcacatatt gaaaaaataa aacgtgggga ttataataaa cttaatcaag
    13921 gacgaagaga gaagaaaaat ggaagcggtt ttagcaatag atttaggtgc gacttctgga
    13981 agagcaatcg ttggttacct ttctgaaaat aaactggtaa tggaagaaat aaatcgcttt
    14041 tctaatctac ctattagagt aaaagggcat ttatcttggg atattgactt tctactagct
    14101 aaaattcttg aaagtatccg cttggctaat actagttaca agattttatc tatcggtatt
    14161 gacacatggg gagttgattt tggactgatt gataatgaag gtaagctgtt attacaacct
    14221 gttcattatc gtgatgaaag aacaaaggga gtgttaaagg aaatatctga aatgactgaa
    14281 ttagaaaaac tgtattcaga gacaggaaat cagattatgg agataaatac cttgtttcaa
    14341 ctctttaagg cacgtcaaga atctcctgac tctttctata agaccaataa gattctttta
    14401 atgccagatt tgtttaatta tctcttgaca ggtaagtttg ctacagaaaa aagcattgct
    14461 tcaacaactc aattatttga tcctaggagt caaaattgga atcagaatat cttaaaacta
    14521 tttgaattgg attcatcttt acttcctgaa attgtttcag agggaaatgt tcttggaagg
    14581 ataaaaaagg agtatggttt aggcgatatt cctgttgtga atgtttgtag tcatgataca
    14641 gcaagtgcga ttgtctcagt acctaagaca gaaggtagtt tatttatttc atcaggtact
    14701 tggtctttgg ttggagtgga acttacttca ccgattctta ctaccgaatc cttcagttat
    14761 ggatttacaa atgaagtcgg taaagatgga gtgattacat ttctgaagaa ttgtacaggg
    14821 ttgtggatca tagaggaact aagacgttca tttgaacgaa gagggaaagc ctattctttt
    14881 gatgatatta ggacaatggt ggagaaagaa aaagaaaatc ttcctctgat tgatactgaa
    14941 tcaactgaat ttgcaacaga atctgatatg cacaagactt tgacagaata tctagcttat
    15001 catcatgaaa ctagagagtg gacagatgga caactattta agattgttta tgaaagccta
    15061 gctgaaacgt ataggaaagc gatagagtta ctagaagaac taactcataa ggtttataag
    15121 aggatatatg tgattggagg aggtgctaga gccagttact ttaaccaaat gattgctgat
    15181 agaactggta aagaggttct tacaggttcg actgagggta cagctgtggg gaatattgtt
    15241 gtgcagctca tagctatggg acaattaaaa gggatggaag aagctcacca tgttattgag
    15301 gagttcctac aattagagag ttattactcc caaaagaatt aaaaagattg agagtttgta
    15361 aatttgcctc cctccccctt cttaagtttt gtacaagaag gggggataat tggtgaattg
    15421 aaaaatattt agtgttttga tatgaggagg acaagaatgt cagatgtaaa acaagaatta
    15481 attaagtatg gcaagaagct agtagaaaca gatttgacga aaggaacagg tgggaatctc
    15541 agtgttttcg atcgtgaaaa acaattgatg gcaattaccc cgtcgggtat tgatttcttt
    15601 gaaatcaaag aatccgatat tgtagtgatg gatattaatg gaaatgttgt agagggagaa
    15661 cgcttgccat ctagcgaatg gtatatgcat ttgattcaat accaaactcg tgatgatatc
    15721 gatgcaatta tccatgctca tacaacttat gcaacagtat tagcttgtct cagagaacca
    15781 cttccggcta gtcattatat gattgcagtg gcagggaaag atgttcgggt agctgagtat
    15841 gcaacatatg gtacgaaaga attggctgtg aatgcagcta aagcaatgga aggtcgtaga
    15901 gcagttttac tagcgaatca tggaatttta gcaggtgcac aaaatttatt gaatgcattt
    15961 aatattgttg aagaagttga atattgtgca aaaatttatt gtttagctaa gaattttgga
    16021 gagccagtag ttcttcctga tgaggagatg gatttaatgg cagaaaaatt taaaacatac
    16081 ggtcagagaa aatagggagg atattaatgt taaaacatat accgaaaaat atttctccag
    16141 atttattgaa gactttaatg gaaatgggac atggagatga aatagtatta gctgacgcga
    16201 attatccttc tgcctcatgt gcaaataagc taattcgttg tgatggtgta aatattccag
    16261 aattattaga ttccattctg tatttaatgc cattagatag ttacgtcgat agttcaattc
    16321 agtttatgaa cgttgtttcg ggtgatgata ttcctaagat atggggtacc tatagacaga
    16381 tgattgaagg tcatggtaca gatcttaaaa cgattactta tcttagaaga gaagactttt
    16441 atgaacgtag taagaaagct tatgctattg ttgctacagg agaaacttca ctttatgcta
    16501 atattatcct taagaaagga gtagttgttg aaagagaaaa tgtttaatag aggaatttta
    16561 gttgccagtc atggtaattt tgctagcgga gctctcatga ccgcagaaat gtttgttggt
    16621 gagacaacaa atgatagagt taggacatta ggtttgatgc ctggagagaa tattgtagag
    16681 tttgaacatt attttaaaaa tcaagtaaat gaactgttag actcaaatca agaggttatc
    16741 gttttgactg acttgattgg aggaagtcct aataatgtgg ctttgtcacg gtttttaaat
    16801 ttggattcag ttgatattgt aacagggttt aatatccctc tcctagtgga attaatatca
    16861 agttatgatt caaaaatcaa tttagaagaa attgttcaaa atgctcaaaa tagtttgttt
    16921 aatgttaaac aacaacttaa cgtagaggag gaagaggatt tatgtctata gagtttgttc
    16981 gtattgatga ccgtctggta catggtcaag ttgtcactac gtggctaaaa aagtatgata
    17041 ttgagcaagt tatcattgtt aatgatcgca tctcagaaga taaaacacga caatctattt
    17101 taaagatttc tgcaccggta ggtttaaaaa ttgttttctt tagtgtaaaa cggtttgtgg
    17161 aagttttaaa ctctgtgcca ataaaaaaga gaacaatgct gatatataca aatccaaaag
    17221 atgtgtatga ttctattgaa ggaaatttaa aattggagta cctcaatgta ggacagatga
    17281 gtaaaacgga ggaaaatgaa aaggtaacgg gaggtgtagc tctaggtgaa gaagacaaat
    17341 attattttaa gaaaatagtt gataagggaa cgagagttga aattcaaatg gttcctaatg
    17401 ataaagttac aatgttggaa aaatttttat aaaaataatt taaggaggta cagtatatgc
    17461 tattcacaca agcattactg gtgacattag ttgggattat tgccactatt gactataatg
    17521 gaccgttatt tatgattcac cgtccgttag ttacaagtgc aatggttggc ttagtcttag
    17581 gagatttcac ccaaggtgtt cttattggtt cagctcttga attaacttgg ctcggtgtaa
    17641 caggtattgg aggttacact ccaccagata ctatttcagg tgcgattatt ggtactgcat
    17701 ttggtatttt gtctggtcaa ggagaaactg ctggtatcgc tatagcagtt ccaattgcag
    17761 ttgctaccca acagttggat gttcttgcaa aaactttaga tgtttatttt gtgaaaaaag
    17821 ctgataatga tgctaaaaac ggagattatt caaagatcgg tttttatcat tattcaagtt
    17881 tggttttaat cactttattt aaaattgtac caattttcct agctattatg cttggagggg
    17941 aatatgtggc agacttgttt gctaaggttc caccaatcgt tatgcaggga cttaactctg
    18001 caggtgcttt actaccttca attggttttg gtatgctttt aaatatgatg ctcaagaaaa
    18061 atatgtgggt attcttgttg attggattca tttgttctgt gtatggagga atgtcaacca
    18121 ttgggatctc actagttggt attgcggtag catacttcta cgatatgatt ggaagtaaac
    18181 cacaagaaac aacttcaagt agtgatgttg aggaggatct tgatctatga tgaataataa
    18241 agtaactaaa gttgaactta aaaaagtttt caaacgaagt tttatgtatg gttcttcatg
    18301 gaactatgag agaatgcaga acctaggttt tctatataca attcttccag tattgaaaaa
    18361 actataccca gacaaagatt cagcttctcc tgcaatgaaa cgtcaccttg agtttttcaa
    18421 tactcatcaa acagcggcac catttattct tggagttact tccgctatgg aagaacaaga
    18481 aggaaatgaa ggtgcagctt caattactgg tattaaagtt ggcttgatgg ggccgctggc
    18541 tggtctagga gatagtttgt tctggctgac actagttcct atctgtttta gtattggtgc
    18601 gtcttattct aaagacggcg gtgctttagg tatctttatc gccttaatat tgtttaatat
    18661 tattaatatt cctgttaaat atttcggttt gaaatatggg tatactaagg gttctagtct
    18721 tatccaagaa aataatacaa aaggaacatt gaatcgcgtt acgagtatgg cgacagcatt
    18781 agggctagta ctagtgggtg gtttgattcc atcaatggtt ggtattaatt ttggattaga
    18841 atttaaacag ggggaacttg ttatttctgt tcaagaaatg attacaaaat taattccagg
    18901 atttatccct atggctttga ctttattaat gtgtaaatta attagaaaag gaaagaatcc
    18961 ggttgtacta atctttagtg ttatggctat tggagttatc ctagttgttt taggaatttt
    19021 gaagtagtag aaagtgtgga ggtggtattt gggatatcac ctccattttg gaagagaggt
    19081 aaagagtgaa attatggtat aagaaagctg cctcaaattg gaatgaagcc ttgccgattg
    19141 ggaacggtca tttaggtggt atgatttatg gttcagctac aaaagaatgt attcaactaa
    19201 acgatgagac tatttggtat agaggaaagt cagatagaaa taatccagac tcactattgc
    19261 atcttaaaaa aattcgggaa tatcttttag atggagaaat tcagaaagcc gaagaattga
    19321 taaagttaac aatgtttgct accccaagag atcaaagcca ctatgaatta cttggggaac
    19381 tttacattga gcatatagat attcagtctt gtgctctttc attgtatgaa agagagctag
    19441 atttagatac agctatttct aatgttgtgt ttgatcctaa tagttgtaat ttacaaataa
    19501 aaagagaata ttttacgagt tttaataaga atattttatg ttgccgtata gtgtcatcag
    19561 ttcaaaacac attaaattta aacattaatt tgggtagaaa taaacggttt aatgacgaag
    19621 tatctaaact ggattcaagt acaattttaa tgtcggcctc tgctggaggt agaaaaggtg
    19681 ttcagtttaa agtagtatgt cattctaaag ttacggatgg tgaagtaagt gtattgggag
    19741 agacaatagt tattcggaat gctacagagg tatttcttta tctcaaatca atgacgaatt
    19801 attggggaaa tatagatatt tcttctcttc agggagaatt tagtagtatt gattacttta
    19861 cagaaaaaga tgaacatgta aaaaaatatc aggagcaatt taatagagtt gattttaaac
    19921 tagactatag taaagattgt cttagcattc caacgaatct acttcttgaa aacactaaaa
    19981 agtatagtaa ctacttgact aacttgttat ttcattatgg aagatatctg ttaatatcgt
    20041 ctagtcaacc gaatggttta cctgccaatc ttcaaggaat atggtgtgat gaattaaatc
    20101 caatttgggg ttctaaatat acgattaata ttaatactca aatgaattat tggatggtag
    20161 gtccatgtga tttaccagaa gtagaatatc cattatttga tatgctcgaa agaatgagag
    20221 aaccgggaag actaaccgct aagaaaatgt atggagctag aggttttaca gcacatcata
    20281 atacggatgg ttttggcgat acggctcccc aatctcatgc catgggggct gcaatttggg
    20341 tattaactat tccatggtta tgtactcata tttgggaaca ctatttatat ttccaagatg
    20401 agcgtattct tacggaacat tttgaaatga taaaagaagc atttcttttc tttgaagatt
    20461 atttatttga ggtggatggc tacttgatga caggtccaag tgtctcacca gaaaataaat
    20521 atcgcttaaa aaatggtatt gaaggaaatg cttgtctatc atctacaatt gataatcaaa
    20581 ttctaagata tttttgtgat tcatgcattg gcattgcaaa acaattagga gacaattcgg
    20641 attttattag tcgtgtgaag gagttaaaaa agaaactacc taaaacaaaa ataggtagta
    20701 atgggcaaat ccaagaatgg ttagaagatt atgaagaagt agagcctggg catagacaca
    20761 tttcacctct atttgggctt tatccttata atgagattga tattcataaa actccggaat
    20821 tagcagaagc agctaaaatc actatcaata ggagattatc aaacgctaac tttttatctt
    20881 cacaggagag ggagcaagcg attaataatt ggttagtaag tggtttgcat gctagtacac
    20941 aaacaggttg gagtgctgca tggctgattc atttttttgc gagactatat caaggtgaac
    21001 ctgcttataa ccagattaat ggtttgttaa ataatgcgac tcttggcaat ttatttcttg
    21061 accatccacc atttcaaatt gatggtaatt taggtttggt gagtggaatt tgtgaattat
    21121 tagtacagag ccatcataat tggttatcac taattccagc tttaccttct gcttggtcag
    21181 aaggagaagt gaaaggtttc agagtaagag gaggatataa ggtatcgttt gcttggaaaa
    21241 acggggatat aacattccta aaattggaag gaggaaacaa agatcaaaaa gtaagagtaa
    21301 gaatatatgg caaaaatact gatgtacaaa atattgaatt ggtatttaat tcagaaaaaa
    21361 ttattgagtt aaatttttag gtataagtca tgaataaaga aaaaataaaa agaaaattaa
    21421 tcacaatact gtttgtatgt attgggatgt tatgttttgg attgttagca ggagttaagg
    21481 ctgataatcg tgttcaaatg agaacgacga ttaataatga atcgccattg ttgctttctc
    21541 cgttgtatgg caatgataat ggtaacggat tatggtgggg gaacacattg aagggagcat
    21601 gggaagctat tcctgaagat gtaaaaccat atgcagcgat tgaacttcat cctgcaaaag
    21661 tctgtaaacc aacaagttgt attccacgag atacgaaaga attgagagaa tggtatgtca
    21721 agatgttgga ggaagctcaa agtctaaaca ttccagtttt cttggttatt atgtcggctg
    21781 gagagcgtaa tacagttcct ccagagtggt tagatgaaca attccaaaag tatagtgtgt
    21841 taaaaggtgt tttaaatatt gagaattatt ggatttacaa taaccagtta gctccgcata
    21901 gtgctaaata tttggaagtt tgtgccaaat atggagcgca ttttatctgg catgatcatg
    21961 aaaaatggtt ctgggaaact attatgaatg atccgacatt ctttgaagcg agtcaaaaat
    22021 atcataaaaa tttggtgttg gcaactaaaa atacgccaat aagagatgat gcgggtacag
    22081 attctatcgt tagtggattt tggttgagtg gcttatgtga taactggggc tcatcaacag
    22141 atacatggaa atggtgggaa aaacattata caaacacatt tgaaactgga agagctaggg
    22201 atatgagatc ctatgcatcg gaaccagaat caatgattgc tatggaaatg atgaatgtat
    22261 atactggggg aggcacagtt tataatttcg aatgtgccgc gtatacattt atgacaaatg
    22321 atgtaccaac tccagcattt actaaaggta ttattccttt ctttagacat gctatacaaa
    22381 atccagctcc aagtaaggaa gaagttgtaa atagaacaaa agctgtattt tggaatggag
    22441 aaggtaggat tagttcatta aacggatttt atcaaggact ttattcgaat gatgaaacaa
    22501 tgcctttata taataatggg agatatcata ttcttcctgt aatacatgag aaaattgata
    22561 aggaaaagat ttcatctata ttccctaatg caaaaatttt gactaaaaat agtgaggaat
    22621 tgtctagtaa agtcaactat ttaaactcgc tttatccaaa actttatgaa ggagatgggt
    22681 atgctcagcg tgtaggtaat tcctggtata tttataatag taatgctaat atcaataaaa
    22741 atcagcaagt aatgttgcct atgtatacta ataatacaaa gtcgttatcg ttagatttga
    22801 cgccacatac ttacgctgtt gttaaagaaa atccaaataa tttacatatt ttattgaaca
    22861 attacaggac agataagaca gctatgtggg cattatcagg aaattttgat gcatcaaaaa
    22921 gttggaagaa agaagaatta gagttagcga actggataag caaaaattat tccatcaatc
    22981 ctgtagataa tgactttagg acaacaacac ttacattaaa agggcatact ggtcataaac
    23041 ctcagataaa tataagtggc gataaaaatc attatactta tacagaaaat tgggatgaga
    23101 atacccatgt ttataccatt acggttaatc ataatggaat ggtagagatg tctataaata
    23161 ctgaagggac aggcccagtc cctttcccaa caccagataa atttaatgat ggtaatttga
    23221 atatagcata tgcaaaacca acaacacaaa gttctgtaga ttacaatgga gaccctaata
    23281 gagctgtgga tggtaacaga aatggtaatt ttaactctgg ttcggtaaca cacactaggg
    23341 cagataatcc ctcttggtgg gaagtcgatt tgaaaaaaat ggataaagtt gggcttgtta
    23401 aaatttataa tcgcacagat gctgagactc aacgtctatc taattttgat gtgattctat
    23461 atgacaataa tagaaacgaa gttgctaaga aacatgttaa taatttgtcg ggtgaatctg
    23521 ttagtctaga tttcaaagaa aaaggagcaa ggtatattaa agttaaatta ctaacgagtg
    23581 gagtgccttt gagtttagca gaagtagagg tttttagaga atcagatggt aagcaatctg
    23641 aagaggatat agataaaata acagaagata aagtagtctc tacaaataag gtagctactc
    23701 aaagttcaac caattatgag ggtgtagctg ctttagcagt tgatggtaat aaagatggag
    23761 attacggaca tcattcggtg actcatacta aggcagattc taacgcttgg tggcaggtcg
    23821 atctgggaga agagtttacg gtttctaaag ttgatattta taatagaaca gatgccgaac
    23881 ctcagcgttt atctaatttt gatgttattt ttctatcttc atcaggagaa gaagttttta
    23941 gaagacattt tgataaagta gttgatggtt tgttatcttt aaaagtacct tctgtagggg
    24001 ctaagctagt caaaatagaa ttaaaatcag cagctattcc gttaagttta gcggaagttg
    24061 aagtctatgg ttcaaagaga actccgaaga aactttctaa tattgcatta accaaagaaa
    24121 ctcgacagag ttcaacggat tacaatggtt tttctcgtct agcagttgat ggaaataaaa
    24181 acggagatta tggtcatcat tcagtgactc ataccaaagg agattctcct tcatggtggg
    24241 agatagattt agcacaaacc gaagaattag aaaagttaat tatttataat agaacagatg
    24301 ctgaaattca gagattatca aattttgata ttattatata tgattcaaat aatcatgaag
    24361 tttttaaaca acatattgac agtttagaaa gcaataatct atccatagac ttaaaagggc
    24421 tgaagggaaa aaaggttaga atttctttga gaaacgcagg aattccttta agtttggcag
    24481 aggtagaggt ttatacttat aagtaatttt aaaaattatc atcggtagta tgaaagaaac
    24541 agaaaaataa gaggaaaata gtatgattca acatccacgt attgggattc gtccgactat
    24601 tgatggtcgt cgtcaaggtg tacgcgaatc acttgaagtg caaacaatga acatggctaa
    24661 aagtgtggca gatttgattt caagcacatt gaaatatcca gatggggaac ctgtggaatg
    24721 cgtgatttct ccatctacta ttggccgtgt accagaggct gcagcttccc atgagttgtt
    24781 taaaaaatca aatgtttgcg caacaattac agttacacca tgctggtgtt atggtagtga
    24841 aactatggat atgtctccag atattcctca tgctatttgg ggatttaatg ggacagaacg
    24901 cccaggagct gtctatcttg cagctgtact agcttcacat gctcaaaaag ggattccagc
    24961 ctttgggatt tatggtagag acgtccagga agctagtgat acagctattc cagaagatgt
    25021 caaagaaaaa cttttacgtt atgcgcgtgc agctcttgca actggcttga tgagagacac
    25081 tgcttaccta tcaatgggta gtgtttcgat ggggattggc ggttctattg taaatccgga
    25141 tttcttccaa gaatacttag gaatgcgaaa tgaatcggta gatatgacgg agttcacgcg
    25201 ccgtatggac cgtggtattt acgaccctga agagtttgaa cgtgctatgg tttgggtaaa
    25261 agaacatatc aaagaaggag ttgaccgaaa tcgtgaggat ttgattcttt ctaaagaaga
    25321 gaaagaaaaa caatgggaat ttgttattaa gatgttcatg attggacgtg acttaatggt
    25381 tggtaaccca agacttgctg aacttggttt tgaggaagaa gcagttggtc accatgcttt
    25441 agtagctggt ttccaaggtc aacgtcagtg gacagaccat ttcccaaatg gggactttat
    25501 ggaaactttc ctcaatactc agtttgactg gaatggtatt cgaaaaccat ttgtatttgc
    25561 gacagagaat gattcactaa atggtgtgtc tatgctcttt aattatctat taacaaatac
    25621 tccacaaatc tttgctgatg tgcgcactta ttggagtcca gaggctgtta aacgtgtaac
    25681 gggacatact ttagagggtt gtgctgcagc tggcttctta catctaatca actctggttc
    25741 ttgtacattg gatggtacag ggcaagctac tcgagatggc aaacctgtta tgaaaccctt
    25801 ctgggagttg gatgaaagtg aagtgcaggc tatgcttgaa aatacagact tcccaccagc
    25861 aaaccgcgaa tacttccgtg gaggaggatt ctcaactcgt ttcttgacga agggggatat
    25921 gccagtaaca atggtacgtc tcaatcttct aaaaggggtt ggtccagtgc tacaaattgc
    25981 agaaggttat acacttgaac ttcctgaaga tgttcaccat actttagata atcgtacaga
    26041 tccaggatgg ccaactactt ggtttgctcc acgtttgaca ggaaaaggtg ctttcaagtc
    26101 tgtctatgac gtcatgaata attggggagc taatcacgga gccataacat atggccacat
    26161 tggagcagac ttgattacct tggcttctat gttgagaatt cctgtcaata tgcataatgt
    26221 acccgaggaa gatatcttta gacctaaaaa ttggtcctta tttggaacag aagatctaga
    26281 atcagcagac tatcgtgcat gtcagttgtt gggaccgcta cataaataaa aattgtgtat
    26341 ataggaggtg aacttatgtc cctcctatcc ttttaaaaag atttgttaaa caattcacaa
    26401 ataattgaaa acgaatacaa aaactaatat aatgatgtta aatagatagc gcggaggcgc
    26461 aggaggaaaa ttatatggct acattttatg ttccggcagt caaccttatt ggtaaaggtg
    26521 ttgtaaatga agtgggtcct tatatcaagg aacttggcta taaaaaggca cttttggtga
    26581 cagataagta catcgaaggc agtgacattt tacctaaggt tttaaaacca ctggatacag
    26641 aaggaatcga atatgtcatc tttagcgatg tagagccaaa ccctacttgt aagaatgtca
    26701 cagatggggt agctgctttg caagaacatg gatgtgattt tatcatcagt cttggtgggg
    26761 gttctccaca ggatgcagct agttgtattt ctatcatggc tacaaatggt ggaaaaccac
    26821 aggattatga agggcttcat aagtctgcta aaaaaggctt gccagttgtg gctatcaata
    26881 caacggcagg aacttctgca gaaatcacta ttaactatgt gattactgat gaagaacgca
    26941 aggttaagat ggtaatggtt gacaagaata gccttgctct tatctctgtt aatgacccgg
    27001 aactcatgct ttccaaacct aaaggcctga ctgctgctac tggtatggat gctctgactc
    27061 acgctgttga agctttggta acacctggtg cttatgatgt aaccaagaaa ctgtctattg
    27121 gtgctattga gcttatcaag gaatatcttc ctcgtgctgt agaaaatgga tacgatattg
    27181 aagcgcgtga aggtatggtc aatgccatct tccttggtgg tatgagcttt aataatgctg
    27241 gtcttggtta tgttcactca atggctcacc aactcggtgc agtatataat ttgccacatg
    27301 gtgtttgctg tgccatgttg ctaccagtta tagaacgtga aaatgctaaa cgtgtaccag
    27361 aagctttccg caatgttgcc aaagccttgg gccttcatgt ggaaggtaaa tcagatcaag
    27421 agtgtgccga ttatgcgatt gctgagattg agaagctttc tgagacagta ggtattccta
    27481 agaaattgac tgaacttggt attgaagaaa aagatttcga ctttgaatac ctttctaaga
    27541 atgccttgat tgatgcctgc gcaccaggaa atccatttat gccaacctta gaagaaacga
    27601 ttgcctttta taaagagtta ttttagcaag agattagacg acaataggac aattcacctc
    27661 taattattga catttcagga aaaatcgcag taaaaacggc acagccttct ttaaatgtgc
    27721 tataatacta gaaaaatact tgtggaggtt ccattatggc aatatttttc atgatttttc
    27781 tgattgtttg tgtgctccta ttggtgatag tcacactgag tacagtttat gtggttcgtc
    27841 agcagtcggt ggcgattatt gaacgctttg ggaaatacca aaaggttgct aatagcggta
    27901 ttcatattcg cttgcctttt gggattgact cgattgcagc acggattcag ttgcgcttgt
    27961 tgcaaagtga tattgtggtt gagactaaga ccaaggacaa tgtgttcgtt atgatgaatg
    28021 tagcgactca gtaccgtgtc aacgagcaga gcgtgacaga tgcttactat aaactcatac
    28081 gtccagaatc tcagattaaa tcttatatcg aagatgctct tcgctcttct gttccaaaat
    28141 taaccttgga tgaattgttt gagaaaaaag atgagattgc ccttgaggtt caacaccaag
    28201 tagcagaaga aatgaccact tacggctaca ttatcgtgaa aaccttgatt accaaggtcg
    28261 aaccagatgc agaagttaag caatctatga atgaaatcaa tgcggcgcaa cgtaagcggg
    28321 tcgcagcaca agaattggcg gaagctgaca agattaaaat tgtcactgca gctgaagcag
    28381 aagcagaaaa agaccgcctt catggtgtgg ggattgctca acaacgtaag gcgattgtgg
    28441 atggattggc agagtctatc accgaactca aggaagccaa tgttggcatg acagaagaac
    28501 aaatcatgtc tatcctcttg accaaccagt atttggatac cttgaatacc tttgcctcta
    28561 aaggaaatca aaccatcttt ttaccaaata cgccaaatgg tgtggatgat atccgaacac
    28621 aaatcttgtc agcccttcgc gctgagaaga aataatagac taatactctt cgaaaatctc
    28681 ttcaaactac gtcagcgtcg ccttgccgta t
//
LOCUS       DAVAAL010000015        28622 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVAAL010000015 DAVAAL010000000
VERSION     DAVAAL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28622
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.015"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(136..903)
                     /locus_tag="VQM60_000499"
     CDS             complement(136..903)
                     /locus_tag="VQM60_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET1080394.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(900..1766)
                     /locus_tag="VQM60_000500"
     CDS             complement(900..1766)
                     /locus_tag="VQM60_000500"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET1080395.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(1803..3170)
                     /gene="accC"
                     /locus_tag="VQM60_000501"
     CDS             complement(1803..3170)
                     /gene="accC"
                     /locus_tag="VQM60_000501"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET1080396.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(3182..3604)
                     /gene="fabZ"
                     /locus_tag="VQM60_000502"
     CDS             complement(3182..3604)
                     /gene="fabZ"
                     /locus_tag="VQM60_000502"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET1080397.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(3601..4086)
                     /locus_tag="VQM60_000503"
     CDS             complement(3601..4086)
                     /locus_tag="VQM60_000503"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET1080398.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(4089..5324)
                     /gene="fabF"
                     /locus_tag="VQM60_000504"
     CDS             complement(4089..5324)
                     /gene="fabF"
                     /locus_tag="VQM60_000504"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET1080399.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(5346..6077)
                     /gene="fabG"
                     /locus_tag="VQM60_000505"
     CDS             complement(5346..6077)
                     /gene="fabG"
                     /locus_tag="VQM60_000505"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET1080400.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LSEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(6111..7031)
                     /gene="fabD"
                     /locus_tag="VQM60_000506"
     CDS             complement(6111..7031)
                     /gene="fabD"
                     /locus_tag="VQM60_000506"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET1080401.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEATPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(7024..7998)
                     /gene="fabK"
                     /locus_tag="VQM60_000507"
     CDS             complement(7024..7998)
                     /gene="fabK"
                     /locus_tag="VQM60_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET1080402.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(8117..8341)
                     /locus_tag="VQM60_000508"
     CDS             complement(8117..8341)
                     /locus_tag="VQM60_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET1080403.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(8401..9375)
                     /locus_tag="VQM60_000509"
     CDS             complement(8401..9375)
                     /locus_tag="VQM60_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET1080404.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(9375..9809)
                     /locus_tag="VQM60_000510"
     CDS             complement(9375..9809)
                     /locus_tag="VQM60_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET1080405.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGHLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(9903..10688)
                     /locus_tag="VQM60_000511"
     CDS             complement(9903..10688)
                     /locus_tag="VQM60_000511"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET1080406.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            11013..12377
                     /locus_tag="VQM60_000512"
     CDS             11013..12377
                     /locus_tag="VQM60_000512"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET1080407.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            12380..12751
                     /locus_tag="VQM60_000513"
     CDS             12380..12751
                     /locus_tag="VQM60_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET1080408.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGCKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            12965..14239
                     /gene="serS"
                     /locus_tag="VQM60_000514"
     CDS             12965..14239
                     /gene="serS"
                     /locus_tag="VQM60_000514"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET1080409.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(14264..15163)
                     /locus_tag="VQM60_000515"
     CDS             complement(14264..15163)
                     /locus_tag="VQM60_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET1080410.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMTQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(15273..15821)
                     /locus_tag="VQM60_000516"
     CDS             complement(15273..15821)
                     /locus_tag="VQM60_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET1080411.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(16039..17355)
                     /locus_tag="VQM60_000517"
     CDS             complement(16039..17355)
                     /locus_tag="VQM60_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET1080412.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALTQS
                     AFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLMVL
                     LGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(17446..17534)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(17698..20034)
                     /locus_tag="VQM60_000518"
     CDS             complement(17698..20034)
                     /locus_tag="VQM60_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET1080413.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHATEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADRGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDIFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(20138..20686)
                     /locus_tag="VQM60_000519"
     CDS             complement(20138..20686)
                     /locus_tag="VQM60_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET1080414.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(20683..20985)
                     /gene="zapA"
                     /locus_tag="VQM60_000520"
     CDS             complement(20683..20985)
                     /gene="zapA"
                     /locus_tag="VQM60_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET1080415.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            21072..21953
                     /locus_tag="VQM60_000521"
     CDS             21072..21953
                     /locus_tag="VQM60_000521"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET1080416.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGNRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            21957..22571
                     /gene="lepB"
                     /locus_tag="VQM60_000522"
     CDS             21957..22571
                     /gene="lepB"
                     /locus_tag="VQM60_000522"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET1080417.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            22699..25065
                     /locus_tag="VQM60_000523"
     CDS             22699..25065
                     /locus_tag="VQM60_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET1080418.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIVGLSAKNREAFVSTLRLNYGTEMILAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEVRDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDTDFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDTEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDHATQHIGTARKTYLVERFSDLLKN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(25113..26396)
                     /locus_tag="VQM60_000524"
     CDS             complement(25113..26396)
                     /locus_tag="VQM60_000524"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET1080419.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     EDGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(26590..26949)
                     /locus_tag="VQM60_000525"
     CDS             complement(26590..26949)
                     /locus_tag="VQM60_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET1080420.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
     gene            complement(27248..27877)
                     /locus_tag="VQM60_000526"
     CDS             complement(27248..27877)
                     /locus_tag="VQM60_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET1080421.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVRYLKEFRNLNSKNAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     SAVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(27922..28161)
                     /locus_tag="VQM60_000527"
     CDS             complement(27922..28161)
                     /locus_tag="VQM60_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080422.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(28311..28577)
                     /locus_tag="VQM60_000528"
                     /pseudo
     CDS             complement(28311..28577)
                     /locus_tag="VQM60_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
BASE COUNT         8326 a         6567 c         5442 g         8287 t
ORIGIN      
        1 atttttgtaa gcttacatct acagtataca tcttttttgt ctattttata gcccatctcc
       61 tcaactgtca atttttcaac ctgaattgca tttttccata aaaaatgaga cctttctagt
      121 ctcatttagt cattcttagt attttctaaa tcgttgatag cgttcttcca gcaactcttc
      181 tagcggtttt tgtgaaagtc tagccagctc cgtttggagt tcttttttga cactcttaat
      241 cagttcttta ctagaaagtc ctacttcaga aatcacctta tccaccacgt ccatttctaa
      301 cagttcatgc gaagtgattt tcatcagttc tgctgcttcc atggcgcgag taccgtcctt
      361 ccataaaatg gaagcaaagc cttctggact gagaatggca tagatagaat tttccagcat
      421 ccagacacgg tccgcgacag ctagagccag agccccgcct gaaccacctt caccgataat
      481 aatggcgata ataggaactt tcaggtcact catttccatg agattgcgag cgatagcttc
      541 cccttgacca cgttcttccg ctccgacacc aggataagca cctgctgtat tgataaaggt
      601 cacaactgga cggccaaatt tctcagcctg tttcatcaac cgcagtgcct ttcggtagcc
      661 ttctggatgt ggttggccaa aattccgttt gaggttgtct tgcaaactct tgcctttttg
      721 gataccaacc actgttacag cttggtctcc aagccaacca ataccaccaa caactgcacc
      781 atcatcacga aaagaacggt caccatgtaa ttggataaat tcatcaaaaa tgcctgtagc
      841 aaagtccaag gttgtcaagc gactctgctc acgcgcttct ctgactattt ttgcaatatt
      901 catctaggac tccctccatg caatctgact aggctagcaa tcgtatctgg taagtctctt
      961 cttttgacaa tagcatccac aaagccatgt tctaatagga attctgcctt ttggaaatcc
     1021 tcaggcaagc tttcacgaac cgtattttca atcacacgac gtccagcaaa accaaccaag
     1081 ctctgtggtt cagccagaat gatatcgcct tccatagcga aagaagctgt cacaccacca
     1141 gtcgttggat ctgtcaaaat ggtcaggtaa aagagaccag catttgaatg gcgtttaacc
     1201 gccgcagaga tcttagccat ctgcatgaga ctcatgattc cttcctgcat acgggctcca
     1261 ccagaggctg tgaataggac aactggcaat ttttcgacag tcgcatactc aaacaaacga
     1321 gtgatttttt cacctacaac cgtacccata gaagccatga taaagttaga atccataatc
     1381 ccaagagcca cagtctgacc tttaataaga gcagttcctg tcacaacggc ttcatgcaga
     1441 cctgtttttt cacgcataga tgccagtttc ttttggtaac cagggaaatg caagggatcc
     1501 ttgctttcaa tccctgtaaa caattctttg aaggttccca tatcaatcgt caaagccaag
     1561 cgttcttggg cagaaatacg aaaggtatag ctacagtgcg gacagatacg ttcacttccc
     1621 agatccttct gatagatggt atgcttacag cctggacact gggaaaataa ttcatctgga
     1681 acctctggct tagcttgagg tttttcccta accgaacgat tgggattgat tcgaatatac
     1741 ttatcttttt tactaaatag agccattgat tccccttttc ggtttaaact cttaaagtca
     1801 ttttattctt tttcttgata tttaggtaag aaggtttcca tcaagaagga agtatcataa
     1861 tccccagcaa tgacattgcg atctgaaatg aggtcaagct ggaaatctgc attggtctgc
     1921 actccttcaa tctctaattc atagagggca cgttgcattt tcatcaaggc gtcaaaacga
     1981 ttttcgccgt gtactatgat tttggcaatc atactatcat aataaggcgg aatggtataa
     2041 cctggataaa ctgctgaatc cacgcgcaag ccaactccac cacttggcag atagagatta
     2101 gtaatcttac ctggacttgg agcaaagtta aaggctgggt tttctgcatt gatacgacac
     2161 tcgatggcat gaccgcgtag gacaatatct tcttgcttaa cagacaaagg ctgacctgcc
     2221 gcaatgcaaa tctgttcctt aacgatatca acacctgaaa caaactctgt tactggatgt
     2281 tctacctgaa cacgagtatt catctccatg aaatagaaat tgctacttgc ttcatcaaga
     2341 agaaattcaa tggttcctgc attctcatag ccaacaaact ctgccgctcg aacagcagca
     2401 gcacctattt catgacgcag cgtttttccg attgcaatcg agggactttc ttccaaaacc
     2461 ttttggttat tcctttgaag agaacaatcc cgctcaccca agtgaatcac atgtccatgc
     2521 tcatcaccta ggatttgaac ctcaatgtgc cgagctggat agataacccg ttctatgtac
     2581 atggcaccat tgccataatt ggccttggcc tcactagagg cagtttcaaa ggcagaaacg
     2641 aggtcatctg gtttttcaac cttacgaatc cctttaccac ctccacctgc tgaagccttg
     2701 agcataacag gatagccaat tttttcagca acaatcaaag cttcttcaga gttatgcact
     2761 tctccatctg aacctggtat aacaggcaca cctgctttaa tcatctgagc acgcgcattg
     2821 atcttatccc ccatcatatc cataacatga ccagatggac cgataaactt gatacctact
     2881 tcttcacaca tggtcgcaaa tttggaattt tcactgagaa atccaaaacc agggtgaata
     2941 gcttctgcct cagtcaagac tgcagctgat agaactgcat taatattgag ataagactct
     3001 gttgccttgc caggaccaat acaaactgct tcatctgcca aaagcgtatg aagagcttcc
     3061 ttatcagcag ttgaataaac cgctaccgtc gcaatcccca attcacgtgc cgcacggata
     3121 atacgaaccg caatttcacc acgattggca attaaaattt ttcgaaacat ggagaacctc
     3181 cttagttccc aattgcaaaa gtaaggatac cactggctgc aagcttgcca tccacttcag
     3241 cctttgcttc aaccacagct atggtgccac gacgttttac aaaagtcgct gtcataacca
     3301 attggtcgcc tggtacaact tgcttcttga acttaacctt gtccatacca gcgtaaaaga
     3361 ccagttttcc tttattttca ggttttgata actccaacac accagcagtt tgcgccaagg
     3421 cttccataat cagaacacct ggcataactg ggtattgagg aaagtggccg ttaaagaaag
     3481 gttcgttaat ggtcacattt ttgatagcaa caatggtatc ctcgctcact tccaagacac
     3541 ggtccactag aagcatagga taacggtggg gaagagcttc tttgattcct tgaatatcga
     3601 tcatttgata cgtaccaatc ctttaccaaa ctcaaccatt tcttcgttag agacgagaat
     3661 ttccgttacc acaccatcct taggagctgg gatttcattc atgactttca tggcttcgat
     3721 aattaccaat gtttgacctt ttttgacact atcaccaact gtaacgaagg caggtttatc
     3781 tggtccagca gccaagtaaa ccactccaac aagtggactc tctacaagat ttccctcagt
     3841 agccacactt gcttcagctg gagctggaac ttcttctgct acagtctctg ctggagcaga
     3901 tgtaggagct actggactcg gtgttgctag aacgggtgct ggagcgactt gagttgcaac
     3961 ttcaggcaca ggtcttgctt cattcttgct aaactgcaac tcatccgtcc catttttata
     4021 agaaaattct ctcaaacttg actggtcaaa ttgagtcatc aagtctttaa tatcgtttaa
     4081 attcatactt atctattctc ccaacgtttg aaagcaagaa ctgcattgtg gcctccaaaa
     4141 ccaaaagtat ttgaaatagc gtatggaatt tctttctcca aaccttgtcc ataaacgaca
     4201 ttagcttcga tataatctga tacttcactt gtcccagctg tcattggtac aaagttatga
     4261 cgcatagctt cgatggtgac gatagcttct actgcacccg cagcccccag caaatgtcct
     4321 gtaaaagact tggttgatga tacaggtact tccttaccaa gaacagctac gatagcacca
     4381 ctttctcctt tttcattggc aggagttgac gttccgtgag cattgacata ggctacttgc
     4441 tctggagaaa tctcagcttc ttccaaggct agtttgatgg ccttgatagc tccctgacct
     4501 tctggatgtg gagaagtcat gtggtaggca tcacaagtat ttccgtaacc aaccacttca
     4561 gccaggatag tagctccacg tttttcagcg tgttcaagac tttctagaac caacatccct
     4621 gaaccttcac ccataacaaa cccattgcga tccttatcaa atgggatcga agcacgagtt
     4681 ggatcctctg tagtagagag agctgttaag gcttggaaac cagcgatggc aaaaggtgtg
     4741 atagaagctt ctgttcctcc caccaacatc acatcttgga aaccaaactt aatggagcgg
     4801 aaggcatccc caatcgcatc atttgatgaa gagcaggcag tattgataga tttacaaaca
     4861 ccgtttgcac caaaacgcat ggctacattc ccagaagcca tatttggtaa agcttttgga
     4921 agagtcattg gtttgacacg tttgggtcct ttttcatgaa ggcgaagtac ctgatcttca
     4981 atttccttga ttccaccaat accagatgca acgataacac caaaacgatc cctattaaga
     5041 gcctctacat caagattggc atgatttaca gcctcttggg ctgcatacaa ggcatataaa
     5101 gaatagttat caaaacggtt ggtatctttt tttacaaagt atttatcgaa cggaaaatct
     5161 tggatttctg ccgcattatg cacatcaaag tcactatgat caaattttgt aatgccacca
     5221 atgccgattt tcccagttgc taaactattc caaaattctt ctggtgtatt tccgattgga
     5281 gatgttactc cataacctgt taccactact cgatttagtt tcattctttt cacctctagc
     5341 tttcgctaca tacttaagcc accatcaatg gcaatcactt gaccagttag ataatcttgg
     5401 cctgctaaaa atactgtcaa atctgcaacc tgctctgcct gcccaaattc tttcatcgga
     5461 atctgagcta gtgtagcttc cttaatctta tctgataaga tagctgtcat atcagactca
     5521 atcattcctg gagcaatcac attgactcgt atattccgac tagcgacctc gcgtgccaca
     5581 gacttggtaa agccaatcaa gccagcctta gaagcagcat agttagcttg accaatattc
     5641 cccatcaaac caacaacact agacatatta atgatagcac cttctctggc tttcatcatc
     5701 ggtttcaaga ctgattgtgt catattaaag gcaccagtca gattgacctt gagcactttt
     5761 tcaaaatctg cttctgtcat cttgagcata agagtatctt gggtaatccc tgcattgttg
     5821 accaaaacat ctactgaacc cagttctgca atagcttgat caatcatacg cttagcgtct
     5881 gcaaaatctg atacatctcc tgaaatggga accaccttga taccatagtt tgaaaactca
     5941 gagagcaatt cttctgagat tgccccacga ctgtttaaga caatgttggc tcctgcttga
     6001 gcaaacttgt gggcgatggc aagaccaatt ccacgactcg aacctgtaat aaagatattt
     6061 ttatgttcta gtttcatttt tttcctttca aaacttctac ttattttagt ctatttttct
     6121 aaaagtgcta ctaaactcgc ttgatcttcc acatgagcta agtgagcagt ttgatcaatt
     6181 tttttaacaa aacctgacaa gactttcccc ggtccaatct cgataaagtt gcttatgcct
     6241 gcttcttgca tgaccccaat actttcatag aaacgaacgg gttccttgac ctgacgcgtc
     6301 aagagctgag caatgtcctc tttttgcatc acagcagctt ctgtattgcc gactagggga
     6361 caagtaaaat ctgaaaaact tacctgagct agagtttcag ctagtttctg gctagctggc
     6421 tcaaggagag cggtgtgaaa gggacctgac accttaagag gaatcaagcg tttggcacct
     6481 gcttcttgca aaagttcaac cgctcgatca actgcaacca cttctccagc aatgacgatt
     6541 tgtgcaggtg tgttatagtt ggctggagta accactccaa gttcagaagc tttttgacag
     6601 gcttcttcaa tgacctctac tggcgtattg agaactgcta ccatcttgcc agagtcagca
     6661 ggagtcgctt cttccatata ggctccacgc ttagctacca aggcaaccgc atcttcaaaa
     6721 tccaaggcgc cgcttgccac caaggcagag tattctccaa gagacaaacc agcaaccata
     6781 tcaggctgat agcccttttc ttgcaataaa cggtagatag caaccgaagt cgctagaatg
     6841 gctggttgcg tatagcgggt ctgattgagt ttgtcctctt ccgtatcgat gagataacgc
     6901 aaatcataac cgagcacctg actcgctcga tcaatcgttt ctttaacaat cggatactga
     6961 tcatagaaat cccgtcccat ccctagatac tgggcacctt gaccagcaaa taaaaaggct
     7021 gttttagtca tttcttacaa ctcctgtcca gcgagaggct tcttcttgaa ttttcttagc
     7081 ggctccgtaa tacaaatctt ttaggatttc ttcagctgtt tcttctttag aaacaagccc
     7141 tgcgatttga cctgccatga cagagccacc atccacatca ccgtgaacaa ctgctttggc
     7201 tagggcacct gctcccattt gttcaaagat ttctaaatca ggatcttcct gcttaaaggc
     7261 atctttttca gccagttcaa aatctctagt caactgattt ttaatagcac gaacagcatg
     7321 accaaagtgc tgagctgaaa tcgtagtgtc aatatccctt gcttttaaaa ttttctcctt
     7381 gtagtttgga tgggcattcg actcttttgc aactacaaac cgtgtcccca cctgtacagc
     7441 ctctgcacct agcataaagc cagccgcagc accttcacca tccgcaattc ctcctgcagc
     7501 aataacagga atagatatag ctgtggctac ctgtcgcacc aaggtcatgg ttgttaattt
     7561 accgatatgc cccccagctt ccattccttc tgcaataaca gcgtctgcac cgattttttc
     7621 catgcgttta gctaaagcga cactaggaac aacaggaata acgattatcc cagcttcatg
     7681 gaaacgttcc atatacttgc ttggatttcc tgctcctgtt gtgacaactt taacaccttc
     7741 ttcaataacg agatccacga tatcttccac aaagggagat aagagcatga tgttgacccc
     7801 aaagggttta tcagtcaatg atttgatttt atcaatattg gccttgacaa cttctttcgg
     7861 ggcatttccc ccaccgataa ttcctaatcc tccagccttg gaaacagccc ctgccaaatc
     7921 accatcagca acccaggcca tccctccttg gaaaatagga tagtcaatct tcaataattc
     7981 tgtaatacgc gttttcatag tgcctccaac cttccttgct tacgtaatag ttcgatttca
     8041 ccataatttg acagtcaaac tattacctaa acaagaggga gtgggtttct ccctactcct
     8101 tctactaata ttctgcttat tttgcttgct cttcaacgta agcaaccaag tcaccaactg
     8161 ttttcaagtc attttctgct tcgatttgga tatcaaaagc atcttcgatt tctgagatta
     8221 cttggaacaa gtccaatgaa tctgcgtcca aatcatcaaa agttgattca agtgttactt
     8281 ctgatgcgtc ttttccaagt tcttcaacga taatttcttg tactttttca aatactgcca
     8341 tgataggact cctttaaaat aaatagtttt tttataacaa tgtgttcacc acatgattac
     8401 ctaaattgta agaatgagcg tgccccaggt caagcctcca ccaaagcctg atagaagaac
     8461 agtctggcta ccatctaaag ggatgagacc ttgttctaca cactctgaaa gtaaaatcgg
     8521 gatactggct gcactggtat tgccatattc catcatattg gctggaagtt tggctcggtc
     8581 aacaccaatt tttctagcca tcttatccaa aatacggtca ttggcttgat gaagtagcag
     8641 ataatccaag tctgtcacct ctataggaga ttcatcaata gtctgcttga tagacttggc
     8701 tacatctcga atggcaaaat caaagactgt gcgtccatcc atcttcaaaa acgaatctgc
     8761 actttcttga tctgaaaatg gagaatgcaa acctgaatgc ccataagtta aacactcgct
     8821 gcgacttcca tcgctattga gactctcagc taagaaatgc tcttgctcgc tagcttctaa
     8881 caagacacca ccagcaccat ctccaaacaa cacagctgtt gatcgatccg accaatcgac
     8941 tgccttagag agggtttcac taccaatcac caagcctttt tgaaagcgac cagaagcgat
     9001 aaacttttca gcagttgaaa gagcaaatac aaatccactg caagccgcgg ttaagtcaaa
     9061 agcaaaggct ttattagcgc caatattagc ttgaacacga gcagctgtag agggcatcat
     9121 cgaatctgga gtaatggtag ctaggatgat aaaatccagt tcttctcctg ttattccagc
     9181 ttttgccatc agtttcttag caacctctgt agccaaatca ctggtagatt ctgttcttga
     9241 aatatgcctt tgtcgtattc ccgttcgact tgaaatccac tcatcattgg tatccataat
     9301 ctgagccaag tcgtgatttg taaccacttg ctctggcaca taatgagcaa cctgacttat
     9361 ttttgcaaaa gccattattt caaatcctcc aaaaattggt aaagattagt caagccttta
     9421 cccatgacag caatttcttc ctcgctcatg ccatcaataa ttttttctac catggccttg
     9481 tggaagcgtt tatgcagtct atgaatcaag tgacccttct ttgtcaaatg cagatgcacc
     9541 acacgacgat cctgttctga ccgaactcgc tcaatgtagc ccttacgctc taaattgttc
     9601 aaacttgtcg taacagttcc aagagttacc atcaactctt ttgacacttg acttggagtc
     9661 acatctggag ccttaccgat gacatcaatc gtatgcattt ctttgatgga gatatcctta
     9721 aaacgactac ctctcaagtt cacttcctca attacaagga cattgttaaa tatagatgtt
     9781 aaatattcat tgattcgttg gtagtccatt tttcatatcc ctccttcttc aaaaaacttt
     9841 tacaatcaaa acatttgaca gaaagaatta tatcaaaatt caaagaatga tgcaagtatt
     9901 ttttattttc ctgtaaattt aggtcttctt ctctccgaat gagctcgcac tccttctttg
     9961 aaatcctctg tttgagctaa ggatttctgt aggttcagtt ctaaagtagc gtaaccctgc
    10021 cagtctttaa attggctctc ccaaaccaac ttcttaatgg cagcatagga attacttgaa
    10081 gcacgtctta attttttaag aagctgttct ctcgtctttt caagtttttc agcttcagaa
    10141 acgcggtaaa ccaaccccca ttctagggct ttttctgccg ttaaagcctc tcctgtcata
    10201 gctaattgag cagcacgcgt cacaccgata ctgcggctca agagatgaat tccccctgca
    10261 tctggagcca aaccaacacc aacaaaggct tggataaact tagccttatc cgtcgccaaa
    10321 cagaaatctg ccgcaacagc catattcgct gcggcacctg caacagcacc gtcaacttcc
    10381 attaaaacag gtttggctat ttgcttgatt ttataagaaa tcgtattgac caactctgcg
    10441 atttttgtca atgatggaat atcatcctca tccactgccc gcttcatctc taccaaatct
    10501 cccccaactg agaagacctt tccattggca ttgattaaga taaaatgcac agctggattt
    10561 tcttctgcca aagtcagagc ttctaaaatc tcctcacaca tgggaatatg aaaaccattt
    10621 gcgacctcag gacggttcaa ggtaaggatt gccaagtcct cttcaagctg ataaataatg
    10681 tgttccatca gggctccttt ttattttata agcagtttat actcttcgaa aatctcttca
    10741 aaccgcgtca gcttccatct gcaacctcaa aacagtattt tgagctgact tcgtctagct
    10801 tcttagtttg ctctttgatt tttattgagt gttaaattat ttcactgtca aagtatatct
    10861 tttttaggat cagaaagcaa gaaaaaatcg actgaaagtg tctcagccga taagaaaggc
    10921 tcgctttagg ctagaaagca tcttattttc ttcattgaaa aaagcctttc tttctgctat
    10981 aatcgtataa aatacttact ttaggagttc ttatgaaagt tgttaaattt ggaggtagtt
    11041 ctcttgcctc tgctagtcaa ttagaaaaag ttttaaacat cgtcaaaagc gattcagagc
    11101 gtcgttttgt agtcgtttct gcgcctggta aacgcaatgc tgaagatact aaggttacgg
    11161 atgccctgat taaatactac cgcgactatg ttgcgggtaa cgatattagc aagagccaaa
    11221 gctggattat cgaccgctat gctgctatgg ttagtgaatt gggactaaaa ccagctgtgc
    11281 tagaaaaaat ttctaaaagc attcacgcct tggccactct tcctattgaa gaaaatgaat
    11341 ttctctacga tactttccta gcagccggtg aaaataacaa tgccaaattg attgctgcct
    11401 actttaacca aaatggtatc gatgcacgct atatgcaccc tagagaagct gggattgtgg
    11461 tcacaagtga acctggtcac gctcgcatca ttccatcaag ttatgacaag attgaagaat
    11521 tgacaaacac caatgaagtc cttgtcattc ctggtttctt tggtgtcact aaggaaaatc
    11581 aaatctgtac tttctcacgt ggaggttctg atattacagg ttctatcatt gctgctggtg
    11641 tcaaagctga cctctatgaa aactttacgg acgttgatgg tatctttgca gctcaccctg
    11701 gtattatcca ccaaccacac tcgattcctg agttgaccta ccgtgaaatg cgcgagttgg
    11761 cctatgcagg cttctcagtc cttcatgacg aggctcttct tcctgcctac cgtggaaaaa
    11821 ttcctctggt tatcaagaat accaacaacc ctgaccatcc aggtactcgt atcgttctaa
    11881 aacacagtaa tgatgaattt ccagttgtgg gaattgctgg tgactcaggc tttgtcagca
    11941 ttaacatgtc gaaatacctc atgaaccgtg aggttggatt tggccgcaag gttctgcaaa
    12001 tcctggaaga acttaacatc ggttgggaac atatgccaac aggtatcgac gatctttcta
    12061 tcattctccg ttctcgccaa ctaactccca tcaaggaaga agaaatcctg cgtcagttgg
    12121 ttcaaaaggc taaagtagat catgcagaaa tcgaacacga cctttctatc attatgattg
    12181 ttggtgaaaa aatgaagagc catatcggag tgactgctac tgcgacacgc gctctatctg
    12241 aaaacaaaat caacatccag atgatgtctc aaggttctag tgaagtttct attatgtttg
    12301 ttgtcaataa agaccaagag aaagcagcaa ttaaagccct ctacaatgcc ttttttggag
    12361 aaagtaagga agactaagca tggctcaatc acttaacaaa acagtactcc tcagtacgac
    12421 aggcacttcc tacctctcta tcgctgggaa agttgggaaa ttccttgtcg gagatcaggc
    12481 tttggaattt tacccagatg tcaatgtcga acaatttatc cagattcctt ggagccatat
    12541 caatcagatt ggagccaatg tcactggatg caaaatcagc cgccacttcg aagtctttac
    12601 agacaaagga aaattcctct ttgcttcaaa agactcaggt gccattctca aaattgcacg
    12661 cgaaaaacta ggcaatgaca aggtcgtcaa acttccgacc ctgattcaga caattagcca
    12721 aaaatttaaa aatctatttg caaaaaagta gaaactttag tataatcata gcaacggata
    12781 agtaggttat cttcttcttt cagaaagtct gcgggtactg cgagcagata ggagggatag
    12841 gtgaaattct accgttagta acaattacat acacaatcaa gtgcacacct gtgaagttgg
    12901 atggaaccgt ggccctgcca ctccaacgtt ttgtcaggcg tgcttttttc ataaaggagt
    12961 tcttatgtta gatatcaaac gtattcgtac agattttgaa gctgtcgcag aaaaattagc
    13021 tacacgtggt gtagatgctg ctgtcttgaa tgaaatgaaa gaaatcgatg ctaaacgtcg
    13081 taacatcttg gtcaaggttg aaactctcaa agcagaacgt aacacagttt ctgctgagat
    13141 tgcccaagct aagcgcaaca aggaaaatac agatgacaag attgctgcca tgcaaaatct
    13201 atctgctgag gttaaagcct tggatgctga attggcagaa atcgatgcta aattgacaga
    13261 atttacaacg actcttccaa atatcccagc tgacagcgtt cctgttgggg ctgacgaaga
    13321 cgacaatgtg gaagttcgcc gttggggtac tccacgcgag tttgacttcg aacctaaagc
    13381 tcactgggat ctcggtgaag accttggtat ccttgactgg gaacgcggtg gtaaggtaac
    13441 aggcgctcgc ttcctcttct ataaaggcct cggtgctcgt ttggaacgtg ctatctacaa
    13501 ctttatgttg gatgaacatg gaaaagaagg ctatactgaa gtcatcacac cttacatagt
    13561 caaccatgat tctatgtttg gtactggtca gtatccaaaa tttaaggaag atacttttga
    13621 actcagcgat accaactttg tcttgattcc aactgctgaa gttcctctga caaactacta
    13681 ccgtgatgaa atcttagacg gcaaagatct tccaatctac ttcactgcta tgagtccgtc
    13741 attccgttct gaggctggtt ctgccggtcg tgatacgcgt ggcttgatcc gtttgcacca
    13801 attccacaag gttgaaatgg tcaaatttgc caaaccagaa gaatcttacg aagaattgga
    13861 aaaaatgaca gccaacgctg aaaacattct tcaaaaactc aaccttccat accgtgtcgt
    13921 tgctctctct actggagata tgggcttctc agctgcgaag acttacgact tggaagtgtg
    13981 gattccagca caaaacaatt accgtgaaat ctcaagctgt tcaaacacag aagatttcca
    14041 agcccgtcgt gcccaaatcc gttaccgtga tgaagcagat ggcaaggtga aactccttca
    14101 taccttgaac ggttctggac ttgcagttgg acgtacagtg gctgcaattc ttgaaaatta
    14161 ccaaaatgaa gatggttctg tgaccatccc agaagcactt cgtccataca tgggtggagc
    14221 tgaagtcatc aaaccataaa aaataaggtt tagctatttc tagctagacc ttttttcgta
    14281 accaaatcag ataagcacct agtacaaaga ataaaatagt taggcatata atggtttcag
    14341 ccaataccag gtaatccaga aatggaagtt tcaaaattcc ctgagtcatc ttgagcgagg
    14401 tcgctgtgat aatggttggg aaggtgaggg ctgagaaggc tggttgaaaa ccttgtttta
    14461 aaatgttggg cagacgagtt aaaacaaaga aaaagaagga ttgagaagcc aaaatcatga
    14521 caatcaagac ccaagtcggc aggctggttc ctcctactcg aactagagaa gccaagagta
    14581 gagagaaagg agcacagtag attccttctt gtccaagcaa ggctagtggg agtggatgtt
    14641 tctttaaatc gctataaata aggggataga gatagaaggt caagagaaaa ccaaaactca
    14701 aggtcgcata ggcaatttcg ataataccta ccagaggata ggtcaaggca gccactgcta
    14761 tccccacata gagaaccgtc cagcttggag tggcatgaac cctccgccct ggacaagcaa
    14821 acttgatggt aaaaccagca atcaaggtca aatccaagag aaatgaaaac caccaaatcc
    14881 cttgtgctac caaaggaaga taagagaata cgcgaaagac ataggtcgat aaaatcatcc
    14941 cagccatagg aaaggttgcc attcctgaca aaagaggggg cttggtcaat tcttgcttgg
    15001 tttctttcca attaaagaga tgcagaatta gaaagtaaat ccataaaacc aaaccagtca
    15061 gactaaaaag atgggataga accggcaacg tatctaaaat aagatttcca gctcctgcca
    15121 aacctagcaa acaacctgaa aatactaagg ggagtttttt catcctaacc tccaataatc
    15181 atgttagttt cagtataaca taaaagcgct taaatgagga tttaaaaaaa cgaagtccgc
    15241 ttatttcaga cttcatttta ctcagatatg aattaggcat aaggttgcaa ttctggatta
    15301 attggtgtat tagctaagtt gttggcatag ttacagagga ttgctaggct gacaccaaaa
    15361 accacatcca aggcattttg ttgagtgtag ccagcttcta aaaactcaga caaggcttca
    15421 tctcctacac gacccttggt attgataact gccaaggtaa acttagctag ggtatccaat
    15481 ttaggatctg tttcaattgg agtacgattg cgaagagctt gaatcaagtc atcattcatc
    15541 tggatttgtt tgatggaaaa ggctgtgtga cctgcgacac agaaggcaca accattggtc
    15601 acggctgccg tgatttgcac cacttcacgc tcaacgggtg tcaggctgtt gcgacggtgg
    15661 atagatgaga caatttggta ggcttctaaa acagtcgggg cattggccaa gagaccgatt
    15721 aggttgggaa tatagccatt gttgtctttt tctactgttt caagaatttc tttcacttct
    15781 gctggtgctg actctactgt atggatagta aatgttgtca taagatacct cttttcttat
    15841 tattgacact aatattattg gaaaatctta taaaatcctg attcctaagt ttatctaaga
    15901 taaagcttta ttctctcata agattttcgt tgttatatta gtttatcaca cttccaatca
    15961 cttgcataat atatattata tatcaggctg ataaaaatta tttataggca aaaaaatcac
    16021 acgagctgtg tgattccatt atttgtcaaa atacttttta gtttcagcaa taacgactgg
    16081 cgacaagacc aagagggcaa tcaagtttgg cagagccatc aaggcgttaa cgatatctgc
    16141 gataatccag accatatcca actcgataaa tcctcctaac aagaccatga gcacaaaaac
    16201 cacacggtag agccagataa agcgaacccc aaagaggaac tcaaaacagc gttctccgta
    16261 atagttccaa cctagaatcg ttgtaaaggc aaaaagtaca aggaagatgg tcaagagggc
    16321 aggcccaaag tgtgaaaaga ctgttgagaa agctgactga gtcaaggcaa ccccattcaa
    16381 gtcaccactc caaactccag ttaccaagat ggtcaaacca gttagagtac aaatgatgag
    16441 ggtatcaata aaggttcctg tcatggaaat caaaccttgc tctactggtt catttgtctt
    16501 ggcagctgca ataggagcag aacccagacc agattcgttt gagaacacac cacgcgccac
    16561 accattttga atagccatcc gaacgctagc accagcaaat ccacctaccg cagcaagggg
    16621 actaaaagct gaggtaaaga ctaaagcgat tgtgccaggg atttttccaa tattaaagaa
    16681 aataactgta agagttccta agatataaat gatggccata aaaggaacaa cagtagttga
    16741 aaccttagaa atagacttga gtccaccaaa gactgcaatc gctacaaaga cagacaagac
    16801 gagagctgtg atggctggcg aaatcgtcgt tgtattttgg atagattctg caatcgagtt
    16861 gacttgggtg aaggttccga ttcccaagag agcaaccaat actcctgcta ctgcaaacaa
    16921 aacagcaagt ggtcgccact tttctcccat ccctagaagg atataatgca tgggacctcc
    16981 cgctactgca ccatggtcgt ccttggtgcg gtatttgatg gccaagagtc cttccgcata
    17041 cttggtagcc attccaaaga aagccgccat ccacatccaa aatagagctc ctggtccacc
    17101 aaccttgata gccgtcgcaa ctcctatgat atttcctgtt ccaacagttg atgccaaggc
    17161 tgtacacaga gctgcaaaac tggatacatc accatgtccc ttatcctgga taaaaataag
    17221 ctgaaaagcc ttgggcagac gcaaaacctg caagagtcct agccgaatag ttaggtaaat
    17281 ccctgttccg accaataaaa tcaagagggg cggtccccaa gcaaaagcat cgattgattt
    17341 aagcaattct aacatttcct tctcctatcg tttcaacccc aaaagaaaga gcacatgcaa
    17401 gatacatgta ctctggaatg cttagataaa tgctaaaaag cggtctatcc tagctctgtc
    17461 cttttacctg agagtttgag cagttgcctg ccttgcccct tcggtgcctt tacggtctct
    17521 ccagagttcc gtccatttac agtcatggaa aatcaaacga ttccccactt ctattaaact
    17581 tcattcggtg ttggtattta attgattcta attttacaaa aaatgttggc ttttgtcaat
    17641 gtgtttatta gtaaaaatta gttcaacagt ttttacttta taaagtccag aatactgcta
    17701 tcctttaaaa gtgacaatag tcgcaccact gcctccagca ttttgtgggg catagccgaa
    17761 actcttgaca tgtttgtttc tttgcaagta tttggtaact ccttcacgga tgactcctgt
    17821 tccgatacca tggatgatat caacttgagc catattgtta agcaaggctt ggtcgatgaa
    17881 gatatctagc tcattcatgg cttcttcata gcgcttgcct cgaagatcca gtctagcttg
    17941 aggtcctcgc ccagaagttc gtttcacaac attgacctgc ttcttcttga ctggtttttc
    18001 ttgctgggct tgaacaagat caaactcttt ctcttccaag gtcatcttaa tcaagccaac
    18061 ttgggcttcc cagcggccgt ccttgagttg actggtcaag gtaccacgct gaccataact
    18121 gagaaccacg atatcatctc ccacctttgg agctcgtttt ttcttggcct tttgaaggac
    18181 cttgttttta gacaagtcca ctttttcagg agccaatttt ttcaacttgg ccttggcttc
    18241 aatgatttcg tggggcttga gttgggattt actgtggaga tttttgagaa tctggtcact
    18301 ttcacttagg gccatatcca caatctcagc agcctgttca cgcgccttgt taagctcggt
    18361 ttccttttca cgattaagct cgttgtagag tttttttagc gcacggttca tcttgagatt
    18421 ttcttgctcc acctcacgga tattgtccaa gcgtttgcgg ctttccagcg tctgctcttc
    18481 taattgctca atgatacgat tgacgtcatt gtcctgatcg atctgctgac tggcatctcc
    18541 tacgataact tcagataggc ctagacgttt ggcaatttca aaggcattac ttcggccagg
    18601 aacaccctgc ataaagcgat aggtcgggcg aagagttgca gtatcaaact ccatactggc
    18661 attttgcaca aaggctgtct caataccgta ggccttgagt tctggatagt gggtcgtcgc
    18721 catggtcttg atttgacgca ggcgaaggtc ctccagaata gccatggcaa gggctgctcc
    18781 ctcttgggga tcagtaccag cccccaactc atccaaaagt aagagtgaat gttggttgac
    18841 cttgccaaga atatccacga tattggtcat atggctagag aaggtagaca agctctgctc
    18901 aatagactgc tcatctccaa tatcagcaaa gatttcttca aaaataccaa cacgacttcc
    18961 cctgtctgct aaaatcggca atcctgactg ggccatgacc tgtgtcaagc ccagagtttt
    19021 gagcatgatg gtcttcccac ctgtattggg acctgtaatg acaatagctg ttaaatcttg
    19081 accaaaatgg acatcatttg cgacggcatt tttgaccaaa ggatggcaga catggagcag
    19141 ttgaatctct tgattttctg acagctgagg cacgactgct tgtctttctt ggataaatcg
    19201 aaccttggca cgaatcaagt ccagatgacc gataatccaa gcgtcattag caatctcagt
    19261 cgcatgaggg cggacacgct cagaaatttc ttggagaatg cgaagcattt catagcgctc
    19321 atctgctcgc agactagcaa tttcttcgct cagtttgact acctcacgtg gttcgatata
    19381 gacggtgttt ccactagcag aaatatcatg aacgacacct gcaatcttat tgcggtaggt
    19441 gtttttgact ggtaaaacct gacggccatt tctgctagca acaattcctt ctgtcaacat
    19501 ctgcgctttt tgcttgagca agtcttgtaa aacatcgcgt acctgactct cgctatcatg
    19561 tatttttcga cggattcgcg ccaattcttc actggcaaaa ttttcaatga aacccgcatc
    19621 attaaaggcc tgaagatttc cttgtaattg cggaaaatca tgtaatttct caaaccaaag
    19681 ggctaattct tccaagctga cattttccag attggcgtaa aaactttgaa gttctcggct
    19741 ggcaagaagc acgcgtttca agagtaggaa ctcctcgata ttgagatccg ctcccatctc
    19801 caacctcttg cagactcctg caatttcctt agttgagaga atagtaaaat gcggttgctc
    19861 gacgaaaaga gcctgcattt ccttcatctc agcaaaagcc tgtttgattt tatctgcttt
    19921 ggcagtcgga gccagttgtc tcaattgctc caagccctgc tcggtcaaca aatgaggctc
    19981 aaacaaggcc ttgaccttat cgaactctaa tgtttctaat attttcttat tcatctttat
    20041 ttccttgtct ttgaatgtct aggtgtggta gctttttaca ttctgataga ttcgagtcaa
    20101 atctgtaaac aaagggctag gaaaactcct gcccttttta tccgattaaa tttgtcaccc
    20161 agatttgttt gagccaactg gttgttatcg gtatgctctg gatgatgtgt tttgcgacga
    20221 tactcttttc aagaggattt tgtataactg ccatggggat ggtcgccaag attgtcaagg
    20281 ccatttgcaa gacaaataag gtcaccaaca tggacaagat acctgctgaa acttggaaca
    20341 acttaccacc cagttttttg ctaggaatca agtgtaagag aagaccaagc aaacgaccaa
    20401 tgctatagac aatcccaaat acaagcaagt agccgatgcc tgcataaaag accttatcca
    20461 gctgaaagag ttgatccgat gggaaaaaga aagtcccctg accttcctgc gaatttgcat
    20521 aagggagcaa taaatggaat tgctctccaa gccccttata aaactggcca gccataaaag
    20581 ccgatgccat ggctgaaatc aggtaataaa cctgtaagag caggcctctc cgatagccga
    20641 tataaaatcc ccaaaccaag accaatagaa gaaggaatga aatcataagg aatcctcaat
    20701 cttgctctgt tcttgcttac aagtcacaag cttgtgacgg agttcttcta gctcttgctc
    20761 cttatcgtca aattcaatct cacggctgag ctgagttgat aaacagttga ctgccaacaa
    20821 aagagcgatt gtttcatcat ctgcgctagg catttgttct ttaattgctt ggtatttttc
    20881 tgtcgcaacc ttagcgattt cctccataaa aaggttatca tgttcgcttg tcaaggttaa
    20941 cgattttttc ccgaatgtaa atttgaatcg atttagattt gccataaaat tcacctcacg
    21001 atattatacc aaaattcgct aattttgtca gtttttacaa attttactgc ttttgtggta
    21061 caatagaaac tatggcaagt atcacactca caccaagcga aaaggatatt caggcttttc
    21121 ttgaacacta tcaaaccagt ctggctccta gcaagaatcc ctatatccgc tactttttga
    21181 aactacctca agcaacggtt tctatctata cttctggaaa aatcttgctt cagggtgaag
    21241 gggctgaaaa atacgccagt ttctttggct atcaagctgt agagcaaacc agcggacaaa
    21301 atcttccttt aattgggaca gatgaggtgg gaaatggttc ctactttggt gggcttgcag
    21361 ttgtggctgc ctttgtcaca cctgaccagc acgacttttt acgaaaactc ggtgtggggg
    21421 attctaagac tctgaccgac caaaagatcc gtcagattgc tcctattctc aaggaaaaaa
    21481 tccagcacca ggcactcctt ctctcaccca gcaagtacaa cgaggtcatc ggaaaccgct
    21541 acaatgctgt ttcggttaag gttgccctcc ataatcaggc tatctatctc ctccttcaaa
    21601 aaggtgttca gcctgagaaa attgtgattg atgcctttac cagtgctaaa aattatgaca
    21661 agtacttggc acaagagacc aatcgtttca gcaatcctat cagcttagaa gaaaaggctg
    21721 agggcaaata cttggctgtc gcagtttctt ctgtcattgc gcgtgatctc tttctggaaa
    21781 atcttgaaaa tttgggacga gaactgggtt atcagcttcc aagtggagct ggaacggctt
    21841 ctgacaaggt ggctagccag attttgcaag cctatggtat gcagggactc aacttctgcg
    21901 ccaaattgca ctttaaaaac actgaaaaag cgaaaaaacg cttagaaagg taagttatga
    21961 attcatttaa aaatttcttg aaagagtggg gactgttcct cctaattctg tcattactag
    22021 ctttaagtcg tatctttttt tggagcaatg ttcgcgtaga aggacattcc atggatccga
    22081 ccctagcgga tggcgaaatt ctcttcgttg taaaacacct tcctattgac cgttttgata
    22141 tcgtggtggc ccatgaggaa gatggcaata aggacatcgt caagcgcgtg attggaatgc
    22201 ctggcgacac cattcgttac gaaaatgata aactctacat caatgacaaa gaaacggacg
    22261 agccttatct agcagactat atcaaacgct tcaaggatga caaactccaa agcacttact
    22321 caggcaaggg ctttgaagga aataaaggaa ctttctttag aagtatcgct caaaaagccc
    22381 aagccttcac agttgatgtc aactacaaca ccaactttag ctttactgtt ccagaaggag
    22441 aataccttct cctcggagat gaccgcttgg tttcgagcga cagccgccac gtaggtacct
    22501 tcaaagcaaa agatatcaca ggggaagcta aattccgctt ctggccaatc acccgtatcg
    22561 gaacatttta agagacctaa gaggccgaga atcaccaatc tcagcctctt cttctatcgt
    22621 gagaaaatga ttggtaacta tctaaactta ccagaacaga aacacctcaa ctctcaccta
    22681 ttcatgcaaa ggaattttat ggaagtttat ttttcaggaa ctattgaacg gattattttt
    22741 gaaaatccca gcaattttta tcgcatcctc ctcctagaaa tcgacgatac ggacgcagag
    22801 gattttgatg attttgaaat cattgtcaca ggaaccatgg ctgatgtaat tgagggcgaa
    22861 gactatactt tttgggggca aattgtccag cactccaagt atggagaaca actgcaaatc
    22921 agtcgttatg atcgcgcaaa accaactagt aagggcttgg tcaagtactt ttcaagtagc
    22981 catttcaagg ggattggtct caagacagct cagaaaatcg tggataccta tggcgaaaat
    23041 accattgacg aaattttgca acacccagaa aagttagaag gcatcgtagg actctctgcc
    23101 aaaaatcgcg aggctttcgt ctccactctc cgtctcaact acggaacgga gatgattttg
    23161 gccaaactag ccaactacgg cattcccaac aaactagcct ttcagattca agacttttac
    23221 aaggaagaaa cccttgatgt ggttgaaaat tatccctacc agctggttga ggatatcaag
    23281 ggtttgggct ttaccattgc tgaccaacta gctgaggaac taggcatcga aagtcaggct
    23341 cctgaacgct tccgcgccgg tctagttcac agtctttttc aggcctgtat ggaaacaggg
    23401 gacacctatg ttgaagtacg ggatttgctg gaacaaaccc ttactctcct tgagtcttcc
    23461 cgtcccgtgg aactagaccc cagccaagtt gcccaagagc tctcctacct gatcgaagaa
    23521 gacaaggttc agcagattga taccaagatc tttgacaaca gcctcttttt cgctgaggaa
    23581 ggcatccgca gtcacttggt tcgtatcctt gaaaaaggaa aacagaagag tcatgattta
    23641 gaaaccattc aaaaacatat cactactatc gaggaagagc tgggaattga gtatgataac
    23701 attcaaaaac aggctatctg tgatgctatc cagaataagg tctttatcct gacaggtgga
    23761 cctggtactg gtaagacaac tgttatcaat gggatcatcg ctgtttacgc cctgttagag
    23821 ggacttgacc tcaggaagaa aagcaacctg cccattcttc ttgctgctcc aactggacga
    23881 gccgctcgtc gcatgaatga attgacaggc ttgcctagcg cgaccataca ccgccacttg
    23941 ggaatgacag gtgacgatga taccagtcat ctggaagatt acctagatac tgactttatc
    24001 atcgtggatg aattctctat ggtggatact tggctggcca atcaactctt ctccaatatc
    24061 tcttctaaca gtaagatcct catcgtaggt gacagtgatc agctaccttc tgtcagtcct
    24121 ggacaggttc tagcggatct gcttcatatt cctttgattc ctcagactcg cttggaaaaa
    24181 atttatcgac aaagcgaaga atcaaccatc gtcaccctag ctagtcagat tcgacagggc
    24241 atcttgccag ctgatttcac ccaaaaaaaa gctgaccgtt cctactttga aattgctagt
    24301 ggccatattc ctgccaccat tgaaaaaatc ttaggtgccg ccctcagaaa tggtattcct
    24361 gcccgtgata tccaagttct ggctcctatg taccgaggga cggcagggat tgatgctatc
    24421 aatcagctca tgcaagacct gctcaatccc ccacaaaaag atcaactcag ttttgaagct
    24481 cctcagtgcc actatcgtaa gggagacaag gtcattcatt tggttaacga tactgaaatc
    24541 aatgtcttta atggggattt aggagctatc acagacctga ttcctggtaa atacaccgaa
    24601 tcgaaacaag acgagattgt cattgatttt gatggtaatg aagtctctta cccccgtaac
    24661 gaatggtaca agattcgctt ggcctatgcc atgagtattc ataagtctca gggaagtgag
    24721 ttcccagttg tcatcctacc gattactagt gctagccgac gtatgctgga gcgaaatctc
    24781 atctacacag ccattacacg tgccaaaagc aaactcatct tactaggcga attacaagcc
    24841 ttcgaccatg ctacccaaca catcggaact gcccgaaaaa cctatctggt tgaacgcttc
    24901 agtgatctac tgaagaatgt tgaagaaaag caacaagctg tctctgaaac agtcacatca
    24961 agtgcctctg aacaatccta catcctaacc gaagaaaact gggaccgcat cccagccatg
    25021 attgggatta cagacacaga cctcaaagag atttttggaa aatagtgcat cagaaaaccc
    25081 acctgaattg gtgggttttc tgtttattaa gattatttta ctgttgctgt gcttgtaatc
    25141 aattcaacag cttttttgat tgtgatatca tgtttcaaca tgtcagctga aagcaagttt
    25201 tgaacttgtg caacttccat gttgtagtct gctgccaatt gctcaacttc tttttggatt
    25261 tcttcttctg aagcatcaaa tccttcagct ttggcaactg cttcgataac aaggttagtc
    25321 ttagtacgtg actcagcttc tgcttggtat tggttgtgaa ggtcttcttg agtagttcca
    25381 gtaatttgga agtacatgtc agggttgatc ccttgacgtt gcaagttccc aaggaattca
    25441 tttactgaac ggtgaacttc ttcgtggatc atttcttctg gaagttctac gatttcagca
    25501 ttttctacag ctgtatcaat tgctgcacct tcaactgcat ctttgtaagc ttcttcttta
    25561 gcagcagcca attctttgcg gtatttttct ttcaagtcag caagtgtttc aacttcttca
    25621 tcaatgtctt ttgcaagttc atcgtcaaga gctggaactt ctttagcttt tacttcgtgg
    25681 atagttgtca cgaatttagc ttctttacct gcaaggtctt ctgcttggta gtcttctggg
    25741 aatgttacga taacatcaac ggtttcgcca gctgagtgac ctaccaattg gtcttcgaaa
    25801 ccagggatga attgacctga accaagtcca agtgagaagt tttcaccttt tccaccgtca
    25861 aattcaacac cgtcgataga accaacgaag tcgatcacaa cagtgtcgcc gtcttcagca
    25921 gcagcttcct tgataaccaa ttcagccagg ttgttgcgtt cgcgttcgat acgctcttcg
    25981 acatcagcgt cagttacttc tttttctaca tcaactgata cttcaaggtt tttgtagtca
    26041 cccaatttta cttcaggttt tgtaacaact tcagcagcga taacccagtc ttgacctttt
    26101 tccattgaag ttacgtcaat ttttggttgg gcaaccactt caagaccagc ttcttttaca
    26161 gctgcttcat aagcgtttgg caaaagagcg ttcataacgt cttggtaaag tgattcttca
    26221 ccaaattttt tatcgaagat aggacgtgga aggtgacctt tacggaaacc tggaacatta
    26281 agagatttct tcactgactt gaagacacgg tccaattctg gtttgatttg gtcttgagag
    26341 atagtgaaag tcaagacacc acggtttgtt tctttgtttt caaatgatac agacattctg
    26401 tcatttctcc ttaaaatttt ttaaatacag tctattataa cattttctat aaataaaatc
    26461 tagactaatg cagacaatta tagacaacaa gcctttttcc taactaaaac actcataaag
    26521 tatagacaat tttagactac tttggatatc ttttggttag tcattttcca aaactaattt
    26581 atctaatttt taagttatat ttgacttcat cttctcagta acatgagtat aaacacttaa
    26641 aatctttgtt tcattagtat aatctacacg atccattatc gctgttatag gtacttccaa
    26701 ttcagcaagc aaacttatat gcgaatatct aaaaacatga gcagtcaata caaaatcatc
    26761 tttcttaatt tcacttttcc taaacctgtt tttcaaaaag taattgaaac aagttatcat
    26821 taagggagaa ccattatttg taaatatata atcaccttct tctaaaagaa gatattcttt
    26881 taaaatttct aacactctat cactaatacc aattactcta tatgaagcta aacttttcgg
    26941 actatccaaa aacacttcgt tattggacga ttacattata gtaaaacgaa ataagaacag
    27001 gacaaatcga tcaggacagt caaatcaatt tctaacaatg ttttagaagt agaggtgtac
    27061 tattatagtt tcaatctact atacaagacg aaattgaaga aatctttcca ttataaaacg
    27121 catattatca aggtttttaa acacctaata atatgcgttt tttcctattt taaagacttt
    27181 tatccaccta ttatacaaag ttaagtatcc atcacagttt acaattagcc ttcattacct
    27241 aacccattca ccatccttca agagagtaac ctttaagaac cgaataaata aatggcaaat
    27301 ttcgttcatt taatttaact gcactgttaa tttgattagc tccatctcct aaagtatgca
    27361 ctcctactac gcggtgacta gcatcataaa ctgcagatcc actagacccc tctaaagtat
    27421 caacttggta atccaagaac atgccatcat cacttaaaac ttgtttttta tctgtataca
    27481 tttgatgaat tttaaaatta tatgatagat agcctgtgat agtcacagtt attcctgtca
    27541 aatttttttg actagtagga agacccaaag tccctaattt tgcaccaatg ggctcttcta
    27601 gaattaataa agccaagtca tattcccttg cattcttaga atttaaattt ctaaattcct
    27661 tcaaataacg aacttccttt actttgatct ttccaaatag ttcttgactt ggactaacag
    27721 ccggaagaac ataaatatca tccgcttctt tcccatagtc atgtctgtag tagttgtgag
    27781 ccgctgtaat taacacatta tctttaataa aattcgcact tcccctccct tctacattca
    27841 ttccatcggc tactccataa tacttagata aaaccatagc tgaagtcgaa tacggatact
    27901 gtaaagtatt atcaatttta atcaaatcat cattaccgat aatacttctg attgcttttg
    27961 gtagtatgaa ccatacgttg gtgaaatctc agataatgaa gaatcattag actctggacc
    28021 tttttctagt gtctcactta cctcatattc ttcacctgta ctagaaataa cactcaaagc
    28081 agatactgtc gataactggc tagccaataa agtactcgca ataattgaaa tacccaattt
    28141 tttataaaca gttttcttca ttatcgtatc ctcctaatgt aattatagcg tactattcta
    28201 aatttctcaa tctactatag aatcaagaaa tctaccacct tctttaaata ccctccatta
    28261 tcacataaac aggtaaactt ttcaattaat gactgcgctt ttcaatcacg ctagaggtac
    28321 ttgcttgctt ctttgatact aagttcagcc attctttcct tgtttttctc aataaagcat
    28381 gttacccaag tgggattcgt tttggagtag tctcgcagag tccagccaat ggctttattg
    28441 ataaaaaatt ctgtttggtt caagttatga aggagaatct tttccattaa ttgagtattg
    28501 gtcttctctt ttcttaacaa ctggtggtca atagcgacac gtctcagcca gatattatct
    28561 gataggctcc attttatact caatgaaaat caaagagcaa actaggaagc tagccgcaga
    28621 tt
//
LOCUS       DAVAAL010000016        26570 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVAAL010000016 DAVAAL010000000
VERSION     DAVAAL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.016"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(494..586)
                     /locus_tag="VQM60_000529"
     CDS             complement(494..586)
                     /locus_tag="VQM60_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET1080423.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            747..1718
                     /gene="ruvB"
                     /locus_tag="VQM60_000530"
     CDS             747..1718
                     /gene="ruvB"
                     /locus_tag="VQM60_000530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET1080424.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1807..2271
                     /locus_tag="VQM60_000531"
     CDS             1807..2271
                     /locus_tag="VQM60_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET1080425.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            2502..3260
                     /locus_tag="VQM60_000532"
     CDS             2502..3260
                     /locus_tag="VQM60_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET1080426.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            3269..4072
                     /locus_tag="VQM60_000533"
     CDS             3269..4072
                     /locus_tag="VQM60_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET1080427.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            4094..5353
                     /gene="rseP"
                     /locus_tag="VQM60_000534"
     CDS             4094..5353
                     /gene="rseP"
                     /locus_tag="VQM60_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET1080428.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLGQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            5366..7219
                     /locus_tag="VQM60_000535"
     CDS             5366..7219
                     /locus_tag="VQM60_000535"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET1080429.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            7319..8698
                     /gene="bglA"
                     /locus_tag="VQM60_000536"
     CDS             7319..8698
                     /gene="bglA"
                     /locus_tag="VQM60_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET1080430.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            8890..10698
                     /gene="glmS"
                     /locus_tag="VQM60_000537"
     CDS             8890..10698
                     /gene="glmS"
                     /locus_tag="VQM60_000537"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET1080431.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLSLLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            10970..12019
                     /locus_tag="VQM60_000538"
     CDS             10970..12019
                     /locus_tag="VQM60_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET1080432.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(12119..15925)
                     /gene="spuA"
                     /locus_tag="VQM60_000539"
     CDS             complement(12119..15925)
                     /gene="spuA"
                     /locus_tag="VQM60_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET1080433.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGVDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDVHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQEAQA
                     SSVKEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLL
                     KNKKEN"
     gene            16342..16755
                     /gene="rpsL"
                     /locus_tag="VQM60_000540"
     CDS             16342..16755
                     /gene="rpsL"
                     /locus_tag="VQM60_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET1080434.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16775..17245
                     /gene="rpsG"
                     /locus_tag="VQM60_000541"
     CDS             16775..17245
                     /gene="rpsG"
                     /locus_tag="VQM60_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET1080435.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            17670..19751
                     /gene="fusA"
                     /locus_tag="VQM60_000542"
     CDS             17670..19751
                     /gene="fusA"
                     /locus_tag="VQM60_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET1080436.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            19855..24246
                     /locus_tag="VQM60_000543"
     CDS             19855..24246
                     /locus_tag="VQM60_000543"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET1080437.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQTANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            24348..24611
                     /locus_tag="VQM60_000544"
     CDS             24348..24611
                     /locus_tag="VQM60_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET1080438.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            24604..24882
                     /locus_tag="VQM60_000545"
     CDS             24604..24882
                     /locus_tag="VQM60_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET1080439.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            24903..25055
                     /locus_tag="VQM60_000546"
     CDS             24903..25055
                     /locus_tag="VQM60_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080440.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            25077..26318
                     /locus_tag="VQM60_000547"
     CDS             25077..26318
                     /locus_tag="VQM60_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET1080441.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            26328..26558
                     /locus_tag="VQM60_000548"
     CDS             26328..26558
                     /locus_tag="VQM60_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET1080442.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
BASE COUNT         7447 a         5075 c         6262 g         7786 t
ORIGIN      
        1 aatcaggtgg agttttttag ctctatttca ggcttttggg gactattcta aaaataattt
       61 ttcgatattt ttcggtattt ttcggatttt ggtcggggaa ttggcgggga cttttttagc
      121 gaatataact aagaaatagg tctgtggtcg cttcagctag ttcaaactca atttgattgt
      181 aagtgactgt taattgaaga actggaacta ctgtaataat gattttgctt gtctagtagt
      241 ggcgtattaa atgagaagtg acatgtaaga ttgtctcttc gtttacgaaa tcaaaatttc
      301 tatgaaattg atagaatccc taacgttctc ttaatcttaa ccgtcttgtc gtacagcggt
      361 tgaagaaaat gtaaaccttt gacaaaattc gtgaactatg ggataataaa aaggaattga
      421 gtactagttc tatatcatag gctagaaaag accccaagct cacgaccaaa taagcttggg
      481 gtcttttttg acactatttg tccttgttta gccatttatc gactatacga agaacgacac
      541 cgaccacaat tggtccgatg atagttttga gtactaattc catcatgggc tatctcacct
      601 cctttcgtag gcggtgtagc agtgtcgtag aatattatac cacataagtg ctaaacttag
      661 gagtcaccca atcaggtggc ttttttgttt gtggcttggt tttttgatat aatagagcca
      721 tgagtagaat tttagataat gagataatgg gggatgagga gttagtagaa cgcacgctcc
      781 gtcctcagta tttacgtgaa tatattgggc aggataaggt taaggaccag cttcaaatct
      841 ttatagaagc tgccaaaatg cgagatgaag cgctggatca tgtcctctta tttgggcctc
      901 caggtttggg gaaaacaacc atggcttttg tcattgccaa tgaactggga gtcaatctca
      961 agcaaacgtc tggtcctgtt atcgaaaaag cgggtgattt ggtagcgatt ttgaatgagt
     1021 tagagcctgg agatgtcctc tttattgacg agattcatcg cttacccatg tcagtggaag
     1081 aagtgcttta tagtgccatg gaggacttct acatcgatat tatgattggg gctggtgaag
     1141 gcagtcgcag tgttcatttg gagttgcctc cttttacctt gattggtgcg acgactcggg
     1201 ctggtatgct ctcaaatcca ctacgggcac gttttgggat tacaggtcat atggagtatt
     1261 atgcccatgc tgacttgaca gaaattgtcg agcggacggc agatattttt gagatggaaa
     1321 tcactcatga ggcagcatct gagttggccc tacgtagtcg tggaacccct cgtattgcca
     1381 atcgtctcct caagcgcgtg cgcgattttg cccagataat ggggaatggg gtaatcgatg
     1441 atattattac cgataaggct ttgactatgc tggatgttga ccatgaaggt ttggactatg
     1501 tggatcaaaa aatccttcgt accatgattg agatgtacag tggaggacct gttggtctag
     1561 gaactctttc tgtcaatatc gccgaagagc gtgagacagt tgaagacatg tatgagcctt
     1621 acttgattca aaaaggtttt atcatgcgga cacggtctgg acgggtggcg actgctaagg
     1681 catatgagca cttaggttat gaatacagtg aaaaataagc aagaaatctt agaggctttt
     1741 agagaaaatc cagatatgat ggccattctg acgatcatcc gagaccttgg tttgaaagac
     1801 tcgtagttgg cagcaggttc tgtcagaaat ttcatctgga atcttttgtc agacaaatcc
     1861 ccttttgatc atgaaacaga tatagatgtg attttctttg atccagattt ttcttatgag
     1921 gaaaccttat tactggagaa aaagctgaga gaggattttc ctcagtacca gtgggaattg
     1981 aaaaatcagg tctatatgca tcagcacagt cctcacactg cttcctatac cagttctcgt
     2041 gatgctatga gtaagtatcc agaacggtgt acggcagttg gactgcgctt gaatgaagaa
     2101 tcagattttg aactctatgc accttatggt ttggaggata ttttgaattt tcaagttcgt
     2161 ccaactcctc atttcttaga aaatgaagac cgaatggaac tctatcaaac acgtttatcc
     2221 aagaaaaatt ggcaggagaa atggaaaaat ttgattttta aaaatactta aggaaacttt
     2281 aagctaggga gtgtacacta agttcataag ttaagaagac cttaacttaa actcctaaaa
     2341 ctttttcata ataatctccc tataaaaata aagtcgccca atcaggcggc ttattttttt
     2401 gaaaaatggg catggtgcct gagaataaat agcttagtga tagaagaaaa tggggaaata
     2461 tggtataatg aaacgataga tttttgaata ggaataagat catgtttgga ttttttaaga
     2521 aagataaggc tgtggaagta gaggttccga cacaggttcc tgctcatatc ggcatcatca
     2581 tggatggcaa tggccgttgg gctaaaaaac gtatgcaacc gcgagttttt ggacataagg
     2641 cgggcatgga agcattgcaa accgtgacca aggcagccaa caaactgggc gtcaaggtta
     2701 ttacggtcta tgctttttct acggaaaact ggacccgtcc agatcaggaa gtcaagttta
     2761 tcatgaactt gccagtagag ttttatgata attatgtccc ggaactacat gcgaataatg
     2821 ttaagattca aatgattggg gagacagacc gcctgcctaa gcaaaccttt gaagctttaa
     2881 ccaaggctga ggaattgact aagaacaaca caggattgat tcttaatttt gctcttaact
     2941 atggtggacg tgctgagatt acacaggcgc ttaagttgat ttcccaggat gttttagatg
     3001 ccaaaatcaa cccaggtgac atcacagagg aattgattgg taactatctc tttactcagc
     3061 atttgcctaa ggacttacga gacccagact tgattatccg tactagtgga gaattacgtt
     3121 tgagcaattt ccttccatgg cagggagcct atagtgagct ttattttacg gacaccttat
     3181 ggcctgattt tgacgaagcg gccttgcagg aagctattct tgcctataat cgtcgtcatc
     3241 gccgatttgg aggagtttag gaggaaatat gacccaggat ttacagaaaa gaaccttgtt
     3301 tgcagggatt gccctggcta ttttcctacc aattttaatg attgggggcc tcttgcttca
     3361 gatagcaatc ggaatcatag ccatgctagc catgcatgaa cttttgaaga tgagaggtct
     3421 agagaccatg acgatggagg gcctcttgac cctctttgca acctttgcat tgaccattcc
     3481 cttggagaat tacctgactt ttttgccagt tgatgggaat gtggttgcct atagtgtttt
     3541 gatttcaatc atgttaggaa cgaccgtttt tagcaagtct tatacgattg aggatgcggt
     3601 tttccctctt gctatgagct tctacgtggg ctttggattt aatgctttac tagatgctcg
     3661 tgttgcaggt ttggacaagg ctctcttagc cttgtgtatc gtctgggcga cagacagtgg
     3721 tgcctatctt gttgggatga actatgggaa acgaaagtta gcaccaaggg tatcgcctaa
     3781 taaaaccctt gagggtgcct tgggtggtat tttaggagca attttagtaa ccattatctt
     3841 tatgatagtt gacagtacag ttgctcttcc atatggaatt tacaagatgt cagtctttgc
     3901 tattttcttt agcattgctg gacaatttgg tgatttacta gaaagttcga tcaaacgtca
     3961 ttttggtgtt aaggattctg ggaaatttat ccctggacat ggtggtgttt tggatcgttt
     4021 cgatagtatg ttgcttgtat ttccaatcat gcacttattt ggactctttt aatcaaaaga
     4081 cggaggaaac gctatgctcg gaattttaac ctttattctg gtttttggga ttattgtagt
     4141 ggtgcacgag ttcgggcact tctactttgc caagaaatca gggattttag tacgtgaatt
     4201 tgccatcggt atgggaccta aaatctttgc tcacattggc aaggatggaa cggcctatac
     4261 cattcgaatc ttgcctctgg gtggctatgt ccgcatggcc ggttggggtg atgatacaac
     4321 tgaaatcaag acaggaacgc ctgttagttt gacacttgct gatgatggta aggttaaacg
     4381 catcaatctc tcaggtaaaa aattgggtca aacagccctc cctatgcagg tgacccagtt
     4441 tgattttgaa gacaagctct ttatcaaagg attggttctg gaagaagaaa aaacatttgc
     4501 agtggatcac gatgcaacgg ttgtggaagc agatggtact gaggttcgga ttgcaccttt
     4561 agatgttcaa tatcaaaatg cgactatctg gggcaaactg attaccaatt ttgcaggtcc
     4621 tatgaacaat tttatcttag gtgttgttgt tttttgggtt ttaatcttta tgcagggtgg
     4681 tgtcagagat gttgatacca atcagttcca tatcatgccc caaggtgcct tggccaaggt
     4741 aggagtacca gaaacggcac aaattaccaa gattggctca catgaggtta gcaactggga
     4801 aagcttgatc caagctgtgg aaacagaaac caaagataag acggcaccga ctttggatgt
     4861 gactatttct gaaaagggga gtgacaaaca agtcactgtt acacccgaag atagtcaagg
     4921 tcgttacctt ctaggtgttc aaccgggggt taagtcagat tttctatcca tgtttgtagg
     4981 tggttttaca actgctgctg actcagctct ccgaattctc tcagctctga aaaatctgat
     5041 tttccaaccg gatttgaaca agttgggtgg acctgttgct atctttaagg caagtagtga
     5101 tgctgctaaa aatggaattg agaatatctt gtacttcttg gcaatgattt ccatcaatat
     5161 tgggattttt aatcttattc cgattccagc cttggatggt ggtaagattg tgctcaatat
     5221 cctagaagcc atccgccgca aaccattgaa acaagaaatt gaaacctatg tcaccttggc
     5281 cggagtggtc atcatggttg tcttgatgat tgctgtgact tggaatgaca ttatgcgact
     5341 cttttttaga taatcgagga atattatgaa acaaagtaaa atgcctatcc caacgcttcg
     5401 cgaaatgcca agcgatgctc aagttatcag ccatgctctt atgttgcgtg ctggttatgt
     5461 tcgccaagtt tcagcaggtg tttattctta tctaccactt gccaaccgtg tgattgaaaa
     5521 agctaaaaac atcatgcgcc aagaattcga aaagattggt gctgttgaga tgttggctcc
     5581 tgcccttctt agtgcagaat tgtggcgtga atcaggtcgt tacgaaacct atggtgaaga
     5641 cctttacaaa ctaaaaaacc gtgaaaaatc agactttatc ttaggtccaa ctcacgaaga
     5701 aacctttaca gctattgtcc gtgattctgt taaatcttac aagcaattgc cactcaacct
     5761 ttatcaaatt cagcccaagt atcgtgatga aaaacgccca cgtaatggac ttcttcgtac
     5821 acgtgagttt atcatgaagg atgcttatag tttccacgct aactatgata gtttggatag
     5881 tgtttatgat gagtacaaag cagcctatga gcgtattttc actcgtagtg gtttagactt
     5941 caaggctatt attggtgacg gtggagccat gggtggtaag gatagccaag aatttatggc
     6001 cattacatct gctcgtacag accttgaccg ctgggttgtc ttggacaagt cagttgcctc
     6061 atttgacgaa attcctgcag aagtgcaaga agaaatcaag gcagaattgc tcaaatggat
     6121 agtctctggt gaagatacca ttgcttactc aagtgagtct agctatgcag ctaacttaga
     6181 aatggcaaca aacgagtaca aaccaagcaa ccgtgttgtc gctgaagaag aagttactcg
     6241 tgttgcaacg ccagatgtta aatcaattga tgaagttgca gctttcctca atgttccaga
     6301 agaacaaacg attaaaaccc tcttctacat tgcagatggt gagcttgttg cagcccttct
     6361 agttggaaat gaccaactca acgaagtcaa gttgaaaaac cacttgggag cagatttctt
     6421 tgacgttgct agcgaagaag aagtggcgaa tgttgttcaa gcaggatttg gttcacttgg
     6481 accagttggt ttgccagaga atattaaaat tattgcagat cgtaaggtgc aagatgttcg
     6541 caatgcagtt gtcggtgcta acgaagatgg ctaccacttg actggtgtga acccaggccg
     6601 tgattttact gcagaatatg tggatatccg tgaagttcgt gagggtgaaa tttccccaga
     6661 tggacaaggt gtccttaact ttgcgcgtgg tattgagatc ggtcatattt tcaaactcgg
     6721 aactcgctac tcagcaagca tgggagcaga tgttttggat gaaaatggtc gtgctgtgcc
     6781 aatcatcatg ggatgttacg gtatcggtgt cagccgtctt ctttcagcag tgatggagca
     6841 acacgctcgc ctctttgtta acaaaacgcc aaaaggtgaa taccgttacg cttggggaat
     6901 caatttccct aaagaattgg caccatttga tgtgcatttg attactgtca atgtcaagga
     6961 tgaagaagcg caagccttga cagaaaaact tgaagcaagc ttgatgggag ctggttatga
     7021 agtcttgaca gatgaccgta acgaacgtgt cggtgttaaa tttagcgata gtgacttgat
     7081 tggtcttcca atccgtatca ctgttggtaa aaaagcggcc gatggcattg tagaagttaa
     7141 gatcaaggca actggtgaca ccattgaggt tcatgcagat aacgtgcttg aaacgcttga
     7201 aatcctaagc aagaaataaa aactataatc agaagaaaaa caaggaaaaa atgtaactag
     7261 tttttacctt gttttttctg tataatggga gaaatgagta gataaagagg taaatgttat
     7321 gctaagattt ccaaaggatt ttgtctgggg atcctctact tctggaccgc agacagaagg
     7381 acgtgtagct ggtgacggta agggagacaa tctctgggat tactggttcc aagtggagcc
     7441 aaatcgttac tataatggga ttggttcaga taagacatcg actttttatg aaaattggga
     7501 gcgggatatt gagcttttgt tagagactgg tcacacagtc tttcggactt ctattcagtg
     7561 gtcacggatt tttccacaag gctgtggaaa agtcaaccct caaggtgtgg atttttatcg
     7621 taaggtcttt gaggctatta aggctaaagg aattcgtctg ttagtcaatc tctatcattt
     7681 tgatttacct tttgcccttc aagaggatgg tgatggttgg gaaaataagg cgacagtctc
     7741 agcctatgaa gactatgctc gtttttgttt tgagacttat ggagatttag tggatcaatg
     7801 gattaccttt aacgagccca tcgttcctgt agaatttggt tatttttatg atgcccatta
     7861 tccacataag gtggatgcag aggcagctgt taaggtagcc tatcatacac aattggccag
     7921 cagccgggct gttaaggctt gccatgaact tttgcctgat tccaagattg ggattgtcct
     7981 caacttgaca ccggcttatc cacgtagcca gcatcctgct gatgtcaagg cagctcgtat
     8041 tgcggccctt tttcaggccc aatctttctt agatccatct gtcttgggga cttatccaca
     8101 ggagttggta gaaatcttgc atgaacacgg tcttttacct gatgctacag aggaagagtt
     8161 ggaactcatt cgtgataata cggtggactt ccttggtgtg aactactatc aacctttgcg
     8221 tgttatggct cctcgatttg ctaagcatcc agagagtcca ctcttaccag aacattttta
     8281 cgagccttat gtgatgcctg gacgtaaaat caattctcac cgtggttggg agatttatga
     8341 gcaagggatt tatgacatcg cccaaaatat caaggaaaat tatggcaata ttgaatggat
     8401 gttgactgag aatggtatgg gtgttgaagg ggaagaaaaa ttccgtcaag atggaatgat
     8461 tcaagatgat taccgtattg actttgtaaa aggtcatctt cgtgaacttc accgtgccat
     8521 tgaagatggt gccaactgta agggctactt gatttggacc tttattgact gctggtcatg
     8581 gctcaacagc tataaaaatc gctatggttt ggtcgaatta gatttggaaa cgcaagaacg
     8641 tcgtctgaag aaatcaggcc actggttcaa agaattaagc gataataatg gattttaaaa
     8701 aggactctga ctgattatcc caatcggtca gagttttttg cttacaggag cttaatttga
     8761 actataccaa tttttactct tgacaagtga aagaaacaag tatatactgt ttttgctgat
     8821 tctataaaag atgaattaga ctataccaat tttaaggaga aagcacagct tgcctgtgtc
     8881 gtatatacta tgtgtggaat tgttggtgtt gttggaaaca caaatgcaac tgatattttg
     8941 attcaagggc ttgaaaagct tgaataccgt ggctatgatt ctgcgggaat ttttgtccta
     9001 gatggtgctg ataaccattt ggtgaaggct gttggtcgta tcgcagaatt gtctgccaag
     9061 acagctggtg ttgagggaac aactggtatc ggacatactc gttgggcaac tcatgggaaa
     9121 ccaacggaag acaatgctca cccacaccgc tctgagacag aacgttttgt cttggttcac
     9181 aatggggtga ttgaaaacta ccttgaaatt aaagaagaat accttgctgg gcaccacttc
     9241 aaagggcaaa cagatacgga aatcgccgta catttgattg gaaaatttgc ggaagaagac
     9301 ggtctctcag ttcttgaagc ctttaaaaaa gctcttcata ttatccgtgg ttcatatgcc
     9361 tttgccttga ttgactctga aaatccagat gtcatctatg tagcgaaaaa caaatctcca
     9421 cttttgattg gtcttgggga aggctacaat atggtctgct cagatgctat ggctatgatt
     9481 cgtgaaacca accaatacat ggaaattcat gaccaagagt tggtaatcgt caaggctgat
     9541 agcgtggaag ttcaagacta tgatggtaac agtcgtgaac gtgctagcta tactgcggaa
     9601 cttgacttgt cagatatcgg taagggaact tatccttact acatgcttaa ggaaattgat
     9661 gagcaaccaa ctgttatgcg taaactcatt caagcctaca cggatgatgc tggtcaagta
     9721 gtgattgatc ctgctatcat taaggctgtt caagacgcag accgcatcta catccttgca
     9781 gctggaacat cttaccatgc aggatttgct tctaagaaaa tgttggaaga attgacagat
     9841 acaccagttg aacttggaat ctcatctgag tggggctacg gtatgccact tctcagcaag
     9901 aaaccactct tcatctttat cagccaatct ggtgaaacag cggatagtcg tcaagttttg
     9961 gtcaaggcta atgaaatggg aattccaagc ttaacagtga caaacgttcc aggttcaacc
    10021 ctctcacgtg aagccaacta taccatgctc cttcacgcag gtcctgaaat tgccgtggca
    10081 tcaactaaag cctatacagc gcaaatcgca gcccttgcct tccttgcaaa agcagtcgga
    10141 gaagcaaatg gtaatgctaa agcgcaagcc tttgacctgg ttcatgaatt gtcaatcgta
    10201 gctcagtcta tcgaatcaac tctttcagag aaagaaacca ttgaagccaa ggttcgtgaa
    10261 cttcttgaaa caactcgtaa cgccttttac atcggacgtg gtcaagatta ctacgtagcc
    10321 atggaagcaa gtctcaaact caaagagatt tcttatatcc agtgtgaagg ttttgcggca
    10381 ggagaactca agcacggaac cattgccttg attgaagaag gaacgcctgt cttgtctctc
    10441 ttgtcagatc cagttcttgc caaccatact cgtggaaata tccaagaggt cgcagcccgt
    10501 ggtgccaaag tcctcactat cgcagaagag aatgtagcca aagataccga cgatatcgtc
    10561 cttacgaccg tacatccata cctctcacca atttcaatgg tcgtaccaac gcaattagtc
    10621 gcttactttg caaccctcca ccgtggcctc gatgtggaca aaccacgtaa ccttgccaag
    10681 tcagtaacgg tagaataagc taaaaaagtc tagttatcta ggcctttttc ttgtgagcaa
    10741 attggttgct tgtactcaag attcgtgtta tactcttcga aaatctcttc aaaccacgtc
    10801 aacgtcgcct tgccgtaggt atggttactg acttcgtcag ttctatccac aacctcaaaa
    10861 cactgttttg agcagcctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat
    10921 tagaataatt tttcaaattg aagtacagag atagataagg agaatcacaa tggtagaatt
    10981 gggaatttca acatttgggg aaataacgga gcttgaaggg actggacaaa cttacagtca
    11041 tgctgaacgc attcgccagt tggtggcaga gattgagctg gctgacaagg ttggtttgga
    11101 cgtatatggg attggcgagc accatcgggc ggattttgca gtatcagccc cagagattgt
    11161 cctggccgct ggggcagtca ataccaagaa aatccgtttg accagtgcag tcagcattct
    11221 gtcaagcatg gatccgattc gtttgttcca acagtatgcc actatcgatg ctttgtcaaa
    11281 tggacgatcg gagattatgg ctggaagggg ctctttcacg gaatctttcc ctctgtttgg
    11341 ttatgacttg aaagactacg actctctttt tgatgagaaa ttagacttgc ttcagttagt
    11401 caatgaaaag accaagttag actggcaagg tcgattgact caaacgattg ctggtaaaga
    11461 agtttatcct cgtccagttc aggacaaatt gcccttgtgg atagctacag gtggtcatgt
    11521 cgaatcaaca gtgaagattg ctcaggctgg tctaccgatt gtatatgcta ttattggtgg
    11581 taatccacgt tattttaaaa agttgattca ggcttatcgt gagattggaa gcgaagcggg
    11641 ccatgctgac aaagacttga aagtgggagc ccattcttgg gggtggattg cggaagatgg
    11701 tgagcaggcg gtgaaagatt atttccatcc gactaagcaa gtggtggatg ctatttccaa
    11761 agaccgtccg cactggcagg aattgcgtta tgagcaatat ttggagcagg tagggccaaa
    11821 tggcgccatg tttgttggca atccagatca ggtggccgaa aaattgattc gcatgataga
    11881 ggatttagga ttggaccgct tcatgcttca tctaccgctt ggttctatgc ctcatgacca
    11941 ggttctgaga gctattgaac tcttcggaac acaagttgca cccaaagtac gagcttattt
    12001 tgctatgaaa gaggcttaat aaaaaatcct aatcaagttt attaggacaa caaatattgt
    12061 acaaaaatta atcccccgag ctataatgct tgggggattt ttctatagga gggctagttt
    12121 agttctcttt tttatttttt agtaagaaac cgagaccaag gagcgcaagg aggctgattc
    12181 ctgcaaatag gagtttgttt tcgtttttga ttcctgtatt tggaagttca gcttgtttat
    12241 ctggggttgc aggtatattt tccttgctag agttttctac cgattcgttt cgaaccgctt
    12301 cttttacaga tgatgcttgt gcttcttgtg cttcttgtgc tggttgttca gcttgtgaat
    12361 cagctgtagc ttggctaggt ttattttccg catcagctac tttggcatct ggtttagtag
    12421 aatcaggtct agcttctgga gctgggtctt gatgtgcagg gtgagaagct tcattttgat
    12481 gttcaggctt ggaaggggtt gagtctggtt tctcttctgc agttgactca tggctagttc
    12541 cattttgaga gactcgaaga acagtagctg taagggcatt caatttcaag cctttttcag
    12601 tccattcaag tcctttcggg ttggcaattc cgactgatcc tgcttggttt tcatctgcca
    12661 aaacttccgc atttcttaga tgtgcaaagg cagttcccaa attaaattcg cgagcttttt
    12721 catccgcatt gacaaagact gcgtagatat cgccgtttgg agcagtgatt tggtagccaa
    12781 tcactacatc ctctttttcc acaccatttt ggcctgggac agtgatgagg tggacacggt
    12841 ctttgatatc ttgaagactc ttaagtcgga aggcatctgt agattgacga agggcaatca
    12901 aacctttcat atagtcacgg ctcttgacat tttcaggata agctttacca tctgtagcct
    12961 tagtccagtc aaacttgttg actgcatcgc tagaatcgta agaatcatgg atgaagtaag
    13021 gatagtcaaa tgggttgccg tccttatcac gcaacaagtg agatttgttt ggaaccttat
    13081 cctctgctac tggagtcttg taggctgggt cacggaattg tttagtacgt ccatattcct
    13141 gaccggagtg gataaatgga gttccttgag ctgtcaagac catgagattt ccaagtcgta
    13201 aacgacggtg gatttcagca tagttctcag ccttgcttgg gtcttttttg atagactggg
    13261 caatgatgtc aaagagggtc aagttatcat gggctgcgat gtattggatg acatctccag
    13321 ggctgtcagc ttcaaagtta gttggttgag caatgagatt tttaaagatg gtgttgacat
    13381 cacgcttgcc acctgtgata aaggcaggtt gaccttcgtt tggataacca gatttgaggt
    13441 tgttacggat gtcatctgaa aagacagcga cagtatcggt atgtttcatc caatcttggt
    13501 cagcagcttt agtaggcatg ttttcatcac cggcataggt tctccaacct tcaccaagca
    13561 tgatgaggtt tggattgagg gcgcgtgcag ccttgtaagc ttcttcgata gaagcggcgt
    13621 catggtctcc catcatatcg aagcggaagc catccacttt gtaggtatca actaggtatt
    13681 tgatagagtc aactaggagc cgtttggtca tatggtgggt tgtccccaag cgtccaccac
    13741 caaagctagt tcgaggtgtg ccatcggcat ccataaagtg gtagtagttt ggctctaggt
    13801 cttcaaagat atcgactttg gctgtgtggt tataaacgac atctaggata gctcccatac
    13861 cacgtttgtg gatttcgttg atgaggtttt taaattctgc gattcgtttt tctggattct
    13921 taggatcgct tgagtacata ccagtcaagg agaagtagtt ttgagggtca tatccccagt
    13981 tgtagttgct gttgcttgaa gcgtagtcag acaagcgttc atggtttttc aattcattga
    14041 caaagtagta agacaagact ggaaggagct ggatatgggt tacacccaag tctttgagat
    14101 agtctagttt ttcaatgaag gcttcaaaag tcccaaatgg tttggtcaag tcttttgcaa
    14161 tggcaggatc tgaagtgaag tcacgcacat gagcttcgta gataacggcg tcttcacgag
    14221 tcttgaaatt gtgaatctta ccataagtca agtcttgagg tccgagttta gctggatcta
    14281 caaaggcggc tttagccact ttatggacat cgtcaatttt ggcatcgtcg ctattccaag
    14341 cagcaagaga tttagcgtaa ggatcgagtg caagaacagt tttaccttga cgctcgattt
    14401 ggtattgata atagtagcca gtgaaatctg tgattccgag tttgtttgtg ctgtctagag
    14461 tttgtttcca agttcctctt tccccttttt cgagagcgac agttccaact actttgtcag
    14521 ggtcattctt gtcgtagaca acaacagaaa ccttatcagc acttggtgac caaagggtca
    14581 aatcaacttg ttttccttct tcttttaggt cagctcccag tttgccatca tagctgtatg
    14641 tctcatcttt caggcgccag cttgttttgg tagtgaattg gtcggaattg tagctaacag
    14701 tataaggatg ttttgtgtca gagaaatctc cgctgtaggt cactttctta ccagcttcat
    14761 cgattgcaac atcggtaata gttaccttgt ttcctaggtg attagtgatg ttggagtgtt
    14821 tgaggatatc ttctttttta gcaccgacaa gtgttgaaaa gctactttca atgctagaag
    14881 tgcctacgtg ttgggctcct gtcatacgga tatcatggac atagtatgga tttgtgtaaa
    14941 tcgattcatc atcgtctttt aggaaaattt ggctatgatt tttcaaatct gtgaacttat
    15001 aattttcttt acggattttc acgtcgactc cttgtttgct ctcatctagt aataaaaatc
    15061 caaattctct tgcggcttca ttaagaggaa tgtcgatata gcggccatat ttgcctgtag
    15121 ccgtaaagtc tgttccgtca ggccattgag cgctacttgg attttttaca tctccccagt
    15181 accagagaga tttcttgtca tagttgccat ctgtgcggta gtagttgacg cgaacagttc
    15241 ctgcaggctg tggctcgtaa gagaaaacct tgtaatcttg gtctaaccaa gcctcgttca
    15301 tttttggaac tagtttttct acagatttat cgccggttag attttttcca gctgtattgt
    15361 tgatgaggaa gctaattttc ttggcttgtt ctccctttaa tttgacatct aggtaatagc
    15421 cgtagtcatc tttcttggca tccttgaagg acaaagctcc gtttggccag ttttcagatg
    15481 gtttttcaac atcgtcccaa gtccatagtc cttgagcatc cttgttttct tcaggaagtt
    15541 ttttgacatg gatacggaag tagttatctt cgatgggctc ttctgcctta gtttcagttg
    15601 ttactggact agtagaagta gttggctctg ttgcaggttt ttcttctgtt ttctctaggg
    15661 aagcgtttgc atttctaggt gaagaaatat cactttcgtt cttaccagag ccagctgctg
    15721 tatcagtaag aggttgggca agggtggttg tatttctgtt gttgggttcg ttagtggttg
    15781 gtgtactttc gtcggatgag atagttggtg tagccatggc aagcaagaca aggcttgcgc
    15841 cgataaggac agaaccagtt ccatttttga gggaacggat gctgtagacc atttttttct
    15901 cagtgtgaga tggtgttttt ctcataatga ttctccaatc aataaatttc tatatcagta
    15961 tagcatgtag taaaacaata tgcaagcgtt ttctttgatt tgtgaaaaaa attcttactt
    16021 ttgagaagtt ttctgtacta ttttatatat cagtataaat gacggagata aaaatgtcac
    16081 ataagccaag ccgtattatt aggaagatga tgtgttccca gctattttta ttcagaaatt
    16141 gatctctaac attaccaata aaaaagaaaa atatcttgac aagcaaggga aaattttgtt
    16201 acaataataa acggtacttt ttacttttgg tctctcaaga gtgtacaggg acgtgctgac
    16261 aaatgttgca aaagtacaca cagatgatat ctgtcaccaa gtgtatcatc accaaaaata
    16321 aaaaaacaca ggagaatgta gatgcctaca attaaccaat tggttcgcaa accgcgtaaa
    16381 tcaaaagtag aaaaatctaa atcaccagct ttgaacgttg gttacaatag tcataaaaaa
    16441 gttcaaacaa acgtttcttc accacaaaaa cgtggtgttg caactcgtgt tggaacaatg
    16501 acacctaaaa aacctaactc agcccttcgt aaattcgctc gtgtacgttt gagcaacctt
    16561 atcgaagtta ctgcctacat cccaggtatc ggacacaact tgcaagagca cagcgtggtg
    16621 cttcttcgcg gtggacgtgt aaaagacctt ccaggggtac gttaccatat cgtccgtggt
    16681 gcacttgata ctgcaggtgt taacgatcgt aaacaaggcc gttctaaata cggtactaaa
    16741 cgtccaaaag cataaggaaa ggggataaag agaaatgagt cgtaaaaata gagctccaaa
    16801 acgtgacgta ttgccagatc cgctatacaa ttcacaacta gttactcgtc ttatcaaccg
    16861 cgttatgctt gatggtaaac gtggtactgc tgcttcaatc gtttacggtg cttttgagca
    16921 aatcaaagaa gctactggca acgatgcact tgaagtattt gaaacagcta tggaaaacat
    16981 catgcctgta cttgaagtac gtgcacgtcg tgttggtggt tctaactacc aagtcccagt
    17041 tgaagttcgt ccagaacgtc gtacaacact tggacttcgt tggttggtaa caatcgctcg
    17101 tcttcgtggt gaacacacaa tgcaagaccg tcttgcaaaa gaaatcttgg atgctgctaa
    17161 caacactggt gcagcagtta agaaacgtga agatactcac cgtatggctg aagctaaccg
    17221 tgcattcgca cacttccgtt ggtaagatag gatgcgaaag cgttaagaaa gtcccagaga
    17281 aaatagggaa tcgaagcagg ttgcggttgc aaccaatgag attcatcttt ttctccagac
    17341 ttttagcttg agctcaacta aatcatgatg ctaggaacgg taaggatgca aggtgaaaat
    17401 aggaaactga cgcagtattc gacgaatata aggagtttta tctttttcac gcagcatccc
    17461 gttccagctc acatcggcta actaacttta gcccgggttc aaattagcta aatcgattag
    17521 tattagctat aactcagctt accatctcgt aagttgaaac caacaatagc atgaaaacat
    17581 tgagaacggg taggtcctgc ctatccgttt ttattaaaat cgtgttataa tagaatagaa
    17641 atcaaaaata aataggagaa acaaacctca tggcacgcga attttcactt gaaaaaactc
    17701 gtaatatcgg tatcatggct cacgtcgatg ccggtaaaac aacaactact gagcgtattc
    17761 tttactacac tggtaaaatc cacaaaatcg gtgaaactca cgaaggtgcg tcacaaatgg
    17821 actggatgga gcaagagcaa gaacgtggta tcacgatcac atctgctgcg acaacagctc
    17881 aatggaacaa ccaccgcgta aacatcatcg acacaccagg acacgtggac ttcacaatcg
    17941 aagtacaacg ttctcttcgt gtattggatg gtgcggttac cgttcttgac tcacaatcag
    18001 gtgttgagcc tcaaactgaa acagtttggc gtcaagcaac tgagtacgga gttccacgta
    18061 tcgtatttgc caacaaaatg gacaaaatcg gtgctgactt cctttactct gtaagcacac
    18121 ttcacgatcg tcttcaagca aatgcacacc caatccaatt gccaatcggt tctgaagatg
    18181 acttccgtgg tatcattgac ttgatcaaga tgaaagctga aatctatact aacgaccttg
    18241 gtacggatat ccttgaagaa gacatcccag ctgaatacct tgaccaagct caagaatacc
    18301 gtgaaaaatt gattgaagca gttgctgaaa ctgacgaaga attgatgatg aaatacctcg
    18361 aaggtgaaga aatcactaac gaagaattga aagctggtat ccgtaaagcg actatcaacg
    18421 ttgaattctt cccagtattg tgtggttcag ccttcaaaaa caaaggtgtt caattgatgc
    18481 ttgatgcggt tatcgactac cttccaagcc cacttgacat cccagcaatc aaaggtatta
    18541 acccagatac agacgctgaa gaaattcgtc cagcatctga cgaagagcca tttgcagctc
    18601 ttgccttcaa gatcatgact gacccattcg taggtcgttt gacattcttc cgtgtttact
    18661 caggtgttct tcaatcaggt tcatacgtat tgaatacttc taaaggtaaa cgtgaacgta
    18721 tcggacgtat ccttcaaatg cacgctaaca gccgtcaaga aatcgacact gtttactcag
    18781 gtgatatcgc tgctgccgtt ggtttgaaag atactacaac tggtgactca ttgacagatg
    18841 aaaaagctaa aatcatcctt gagtcaatca acgttccaga accagttatc caattgatgg
    18901 ttgagccaaa atctaaagct gaccaagaca agatgggtat cgcccttcaa aaattggctg
    18961 aagaagatcc aacattccgc gttgaaacaa acgttgaaac tggtgaaaca gttatctcag
    19021 gtatgggtga acttcacctt gacgtccttg ttgatcgtat gcgtcgtgag ttcaaagttg
    19081 aagcgaacgt aggtgctcct caagtatctt accgtgaaac attccgcgct tctactcaag
    19141 cacgtggatt cttcaaacgt cagtctggtg gtaaaggtca attcggtgat gtatggattg
    19201 aatttactcc aaacgaagaa ggtaaaggat tcgaattcga aaacgcaatc gtcggtggtg
    19261 tggttcctcg tgaatttatc ccagcggttg aaaaaggttt ggtagaatct atggctaacg
    19321 gtgttcttgc aggttaccca atggttgacg ttaaagctaa gctttatgat ggttcatatc
    19381 acgatgtcga ctcatctgaa actgccttca agattgcggc ttcactttcc cttaaagaag
    19441 ctgctaaatc agcacaacca gctatccttg aaccaatgat gcttgtaaca atcactgttc
    19501 cagaagaaaa ccttggtgat gttatgggtc acgtaactgc tcgtcgtgga cgtgtagatg
    19561 gtatggaagc acacggtaac agccaaatcg ttcgtgctta cgttccactt gctgaaatgt
    19621 tcggttacgc aacagttctt cgttctgcat ctcaaggacg tggtacattc atgatggtat
    19681 ttgaccacta cgaagatgta cctaagtcag tacaagaaga aattattaag aaaaataaag
    19741 gtgaagacta atccgtcctc actctagaag gaagtcactt agtggcttcc ttttgtcttt
    19801 agaaaatacc tctaaatatg gtaaaatagt agaagaataa tgtgaggaaa atgaatgtca
    19861 aatagttttg aaattttgat gaatcaattg gggatgcctg ctgaaatgag acaggctcct
    19921 gctttagcac aggccaatat tgagcgagtt gtggttcata aaattagtaa ggtatgggag
    19981 tttcatttcg tattttctaa tattttaccg attgaaatct ttttagaatt aaagaaaggt
    20041 ttgagcgaag aattttctaa gacaggcaat aaagctgttt ttgaaattaa ggctcggtct
    20101 caagaatttt caaatcagct cttgcagtcc tactataggg aggctttctc tgaaggtcca
    20161 tgtgctagtc aaggttttaa gtccctttat caaaatttgc aagttcgtgc tgagggtaat
    20221 cagctattta ttgaaggatc tgaagcgatt gataaggaac attttaagaa gaatcatctt
    20281 cctaatttag ccaaacaact tgaaaagttt ggttttccaa cttttaactg tcaagtcgag
    20341 aagaatgatg tcctgaccca agagcaggaa gaggcctttc atgctgaaaa tgagcagatt
    20401 gttcaaactg ccaatgagga agcgctccgt gctatggaac aactggagca gatggcacct
    20461 cctccagcgg aagagaaacc agtctttgat tttcaagcga aaaaagctgc agctaaaccc
    20521 aagctggata aggcggagat tactcctatg atcgaagtga cgacagagga aaatcgtctg
    20581 gtatttgaag gggttgtttt tgatgtggag caaaaagtga ctagaacagg tcgtgtttta
    20641 atcaacttta aaatgacgga ctatacttca agtttttcta tgcaaaagtg ggttaaaaac
    20701 gaggaagagg cccagaagtt tgacctcatc aagaagaatt cttggctccg agttcgaggg
    20761 aatgtggaga tgaataactt cacacgcgat ttgactatga acgtacagga tctgcaggaa
    20821 gttgttcact atgagcggaa ggatttgatg ccagaaggtg agcgtcgggt tgagtttcat
    20881 gctcatacta acatgtcgac tatggatgct ttgccagagg tcgaagagat tgttgcaaca
    20941 gctgctaagt ggggacacaa ggcggttgct atcacagacc atgggaatgt ccagtccttt
    21001 ccacatggct ataaggcggc taagaaagcg ggaatccagc tgatctatgg gatggaagcc
    21061 aatatcgtgg aggaccgtgt ccctatcgtc tataacgaag tggagatgga cttatcagaa
    21121 gcaacctacg tggtctttga cgtggaaacg acgggacttt cagctatcta taatgacttg
    21181 attcaggttg cggcctctaa gatgtacaag gggaatgtta ttgctgaatt tgatgaattt
    21241 atcaatcctg ggcatccctt gtcagctttt actacagagt taactggaat tacagatgat
    21301 catgtcaaaa atgccaaacc actagaacaa gttttgcaag aattccaaga attttgcaag
    21361 gatacggtcc tagttgccca caatgctacc tttgacgttg gctttatgaa tgctaattat
    21421 gagcgtcatg atcttccaaa gattagtcag ccagttattg atacgctgga gtttgctaga
    21481 aacctctatc ctgagtataa acgccatggt ttggggcctt tgaccaagcg ttttggtgtg
    21541 gccttggaac atcaccacat ggccaactac gatgcggaag cgactggtcg tctgcttttc
    21601 atctttatca aagaggtagc agaaaaacat ggtgtgaccg atttagctag actcaacatt
    21661 gatctaatca gtccagattc ttacaaaaaa gctcggatca agcatgcgac catctatgtc
    21721 aagaatcagg taggtctaaa aaatatcttt aagctggttt ccttgtctaa taccaagtat
    21781 tttgaaggag tgccacggat tccgagaacg gttctagatg cccatcgaga gggcttgatt
    21841 ttaggttcag cctgttcaga gggtgaagtt tttgacgtgg tcgtttctca aggtgtggat
    21901 gcggcggttg aggtggccaa gtattatgac tttatcgagg tcatgccacc ggctatctat
    21961 gcgcccttga ttgccaaaga gcaggtcaag gatatggagg aactccagac cattatcaag
    22021 agtttgatag aggttggaga tcgccttggc aagcctgttc tggctacggg aaatgttcac
    22081 tatatcgaac cggaagaaga gatttatcgt gaaattatcg tccgtagttt gggacagggt
    22141 gcgatgatta accgaactat cggtcatggt gaacatgccc aaccagcgcc acttccaaag
    22201 gctcattttc gaacgactaa tgagatgttg gatgaatttg cctttttggg agaggaactg
    22261 gctcgtaaac tggttattga aaacaccaat gccttggcag aaatatttga acccgttgaa
    22321 gtcgttaagg gtgacttgta tacgcctttc atcgacaagg ctgaagaaac agttgctgag
    22381 ttgacctata agaaagcttt tgagatttat ggaaatccgc tgccagatat tgttgatttg
    22441 cggattgaaa aagaattaac atccatactg gggaatggat ttgctgtgat ttatctggca
    22501 tcgcagatgc tggtgcaacg ttctaatgaa cggggttatt tggttggttc tcgtgggtct
    22561 gtcggatcta gtttcgttgc gaccatgatt gggattacgg aggtcaatcc tctctctcct
    22621 cactatgtct gtggtcagtg tcagtacagt gagtttatca cagatggttc gtacggttca
    22681 ggatttgata tgccccataa ggactgtcca aactgtggtc acaaactcag taaaaacgga
    22741 caggatattc cgtttgagac cttccttggt tttgatgggg ataaggttcc tgatattgac
    22801 ttgaacttct cgggagaaga tcagcctagc gcccacttgg atgtgcgtga tatctttggt
    22861 gaagaatatg ccttccgtgc gggaacggtt ggtacggtag ctgccaagac tgcctatgga
    22921 tttgtcaagg gttacgagcg agattatggc aagttttatc gtgatgcaga agtagaacgc
    22981 ctcgctcaag gagcggcggg tgtcaagcgg acaacaggcc aacacccggg gggaatcgtt
    23041 gttattccga actacatgga tgtctacgat tttacgcctg tccagtatcc agcagatgat
    23101 gtcacggctg aatggcagac cactcacttt aacttccacg atatcgatga gaacgtcctc
    23161 aaactcgatg tactgggaca tgatgatccg accatgattc gaaaacttca ggatttgtct
    23221 ggtattgacc ctaataaaat tcctatggat gacgaaggcg tgatggcact cttttctggg
    23281 actgatgtac taggggtaac acctgaacaa attggaacgc ctacgggtat gttggggatt
    23341 ccagagtttg gaacaaattt cgtacgtgga atggtagacg aaacccatcc gacaaccttt
    23401 gcggaattac ttcagctgtc tggtctgtcc cacggtactg acgtttggtt ggggaatgct
    23461 caggatctga ttaagcaagg aatagcggac ctatcgactg ttatcggttg tcgggacgac
    23521 atcatggttt acctcatgca tgcgggtctg gaacctaaga tggcctttac cattatggaa
    23581 cgggtacgta agggtttgtg gctaaagatt tcagaagagg agagaaatgg ctatatcgaa
    23641 gccatgaagg ctaataaggt gccagagtgg tatatcgaat cctgtgggaa aattaagtac
    23701 atgttcccta aggcccatgc ggcagcctac gttatgatgg ccttgcgtgt agcttacttc
    23761 aaggttcacc atcctattta ttactactgt gcttacttct ccattcgtgc taaggctttt
    23821 gatatcaaga ccatgggtgc gggcttggag gtcatcaagc gcagaatgga agaaatctct
    23881 gaaaaacgga agaacaatga agcctctaat gtggaaatcg atctctatac aactcttgag
    23941 attgtcaatg agatgtggga acgaggtttc aagtttggta aattagatct ctaccgtagt
    24001 caggcgacag agttcctcat cgacggggat acccttatcc caccatttgt agcaatggat
    24061 ggtctgggag agaacgttgc caagcaactg gtgcgggcgc gtgaagaggg agaattcctc
    24121 tctaaaacag aactacgcaa gcgtggtgga ctctcatcaa ccttggttga aaagatggat
    24181 gagatgggta ttcttggaaa tatgccagag gataaccagt tgagtttgtt tgatgagttg
    24241 ttttaaaaaa ttgcttaata atctattaaa agaggctaac gtatatccaa tagatttaca
    24301 ttagctttct tttttgttaa aatagtctat ggaaagaggg tgagagtatg tcaaagatga
    24361 gtataagcat ccgtctggat agtgaggtta aggagcaggc ccaacaggtg tttagtaatc
    24421 tgggaatgga tatgacaaca gctattaata ttttccttcg tcaggcaatt caatatcagg
    24481 gattaccttt tgatgttaga ctagacgaaa atcggaagtt gctccaagcg ttaacggatt
    24541 tagaccaaaa tcgtaatatg agccagtctt ttgaatcagt ctcagatttg atggaggact
    24601 tacgtgctta agattcgtta tcataaacag tttaaaaaag attttaagtt ggctatgaag
    24661 cgtggtttga aggcagaatt attagaagaa gttttgaatt ttctggttca agaaaaagaa
    24721 catcctgcca gatatcgtga tcattcattg acggcatcca agcattttca aggagttcgt
    24781 gaatgccata cccagccaga ttggcttttg gtttataaag tagacaagtc ggaattgatt
    24841 ttaaatttgc tgaggacagg cagtcacagt gatttatttt aatctatttt aagggggttc
    24901 tcatgaaact aagaatattt gcggaagata agccggctaa gaaggtattt gaatatcaat
    24961 tagaacttgc tgatcgtaca attcttctat cgacagcact cttgtcaggt gctattgctt
    25021 tagcaggaat cttttctgct ttgaaagaaa aataaaaata gaaaagagaa aacagaatgg
    25081 ttttaccaaa ttttaaagaa aatctagaaa aatatgcgaa attgttggtt gcgaacggaa
    25141 ttaacgtgca acctggtcac actttggctc tctctattga tgtggagcaa cgtgaattgg
    25201 cacatctaat cgtgaaagaa gcttatgcct tgggtgcgca tgaggtcatc gttcagtgga
    25261 cagatgatgt gattaaccgt gagaaattcc tccatgcccc gatggagcgt ttggacaatg
    25321 tgccagaata caagattgct gagatgaact atcttttgga gaacaaggct agccgtcttg
    25381 gggttcgttc atctgatcca ggtgccttga acggagtgga cgctgacaag ctttcagctt
    25441 ctgctaaagc tatgggactt gccatgaagc ctatgcgtat cgcaactcaa tctaacaagg
    25501 ttagctggac tgtagcagcc gctgctggac ttgagtgggc taagaaagtc ttcccaaatg
    25561 ctgcgagcga cgaagaagca gttgatttcc tttgggatca aatcttcaaa acttgtcgtg
    25621 tctacgaagc agatcctgtt aaggcttggg aggaacatgc agccattctc aagagcaagg
    25681 ccgatatgct taataaggag caattttcag cccttcacta cacagcgcca ggaacagatt
    25741 taacacttgg tttgccaaag aaccacgttt gggaatcagc tggtgctgtc aatgcacagg
    25801 gcgaagaatt cttgccaaat atgccgacag aagaggtctt cacagcgcct gacttccgtc
    25861 gtgcagatgg ttatgtcact tctacaaaac cgcttagcta caacggaaat atcattgaag
    25921 gcattaaggt gacctttaag gatggacaaa tcgtagatat cactgctgag aagggtgatc
    25981 aggttatgaa agaccttgtc tttgaaaatg cgggtgcgcg tgccttgggt gaatgtgcct
    26041 tggtaccaga tccaagtcca atttctcagt caggcattac cttttttaac acccttttcg
    26101 atgaaaatgc gtcaaaccac ttggctatcg gtgcagccta tgcgactagc gttgttgatg
    26161 gagcggagat gagcgaagag gagcttgaag ctgcagggct taaccgttca gatgttcacg
    26221 tagactttat gattggttct aaccaaatgg atatcgatgg tattcgtgag gatggaacgc
    26281 gggtacctct tttccgtaat gggaattggg caaattaagg agataatatg ttaggaagta
    26341 tgttcgttgg tctcctagtg ggatttttag caggtgctat gaccaatcgt ggagagcgaa
    26401 tgggatgttt tggaaaaatg tttctcggtt ggatcggagc ctttctaggt cacttgctct
    26461 ttggaacttg ggggccagtt ttatcaggaa cagctattat cccagcgatt ttaggagcca
    26521 tgattgtttt agctattttt tggagacgag gaagttaatt tcctaaatct
//
LOCUS       DAVAAL010000017        24531 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVAAL010000017 DAVAAL010000000
VERSION     DAVAAL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.017"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..121)
                     /locus_tag="VQM60_000549"
     CDS             complement(<1..121)
                     /locus_tag="VQM60_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET1080443.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGL"
     gene            complement(160..330)
                     /locus_tag="VQM60_000550"
     CDS             complement(160..330)
                     /locus_tag="VQM60_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET1080444.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            complement(342..908)
                     /gene="amaP"
                     /locus_tag="VQM60_000551"
     CDS             complement(342..908)
                     /gene="amaP"
                     /locus_tag="VQM60_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET1080445.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(992..1156)
                     /locus_tag="VQM60_000552"
     CDS             complement(992..1156)
                     /locus_tag="VQM60_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET1080446.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            1599..3080
                     /locus_tag="VQM60_000553"
     CDS             1599..3080
                     /locus_tag="VQM60_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET1080447.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            3442..4443
                     /locus_tag="VQM60_000554"
     CDS             3442..4443
                     /locus_tag="VQM60_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET1080448.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILKKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            4594..5511
                     /locus_tag="VQM60_000555"
     CDS             4594..5511
                     /locus_tag="VQM60_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET1080449.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            5522..6412
                     /locus_tag="VQM60_000556"
     CDS             5522..6412
                     /locus_tag="VQM60_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET1080450.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            6441..8057
                     /locus_tag="VQM60_000557"
     CDS             6441..8057
                     /locus_tag="VQM60_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET1080451.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFTFISGNGNEDFKFLFAAFDIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            8067..9386
                     /locus_tag="VQM60_000558"
     CDS             8067..9386
                     /locus_tag="VQM60_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET1080452.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAVKHMDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(9596..9668)
                     /locus_tag="VQM60_000559"
     tRNA            complement(9596..9668)
                     /locus_tag="VQM60_000559"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9633..9635),aa:Lys,seq:ctt)
     gene            complement(9933..11204)
                     /locus_tag="VQM60_000560"
     CDS             complement(9933..11204)
                     /locus_tag="VQM60_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET1080453.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALTDLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            11474..11713
                     /locus_tag="VQM60_000561"
     CDS             11474..11713
                     /locus_tag="VQM60_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080454.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVYSNNGLKKYEK"
     gene            11878..12057
                     /locus_tag="VQM60_000562"
     CDS             11878..12057
                     /locus_tag="VQM60_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080455.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            12194..12373
                     /locus_tag="VQM60_000563"
     CDS             12194..12373
                     /locus_tag="VQM60_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET1080456.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            12410..12862
                     /locus_tag="VQM60_000564"
     CDS             12410..12862
                     /locus_tag="VQM60_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET1080457.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            13153..13623
                     /locus_tag="VQM60_000565"
     CDS             13153..13623
                     /locus_tag="VQM60_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET1080458.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNYAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDALMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPKY"
     gene            13642..14730
                     /locus_tag="VQM60_000566"
     CDS             13642..14730
                     /locus_tag="VQM60_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET1080459.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVRKDVVQTHKAMKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            14711..14869
                     /locus_tag="VQM60_000567"
     CDS             14711..14869
                     /locus_tag="VQM60_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HET1080460.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
     gene            14979..16013
                     /locus_tag="VQM60_000568"
     CDS             14979..16013
                     /locus_tag="VQM60_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET1080461.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            16075..18258
                     /locus_tag="VQM60_000569"
     CDS             16075..18258
                     /locus_tag="VQM60_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET1080462.1"
                     /translation="MGPITIPKKTIITLEHLKYLHFSLEETKTYWEKNNIIDGILISI
                     YYNRIVAKSNRINGYFNVGGGNPFPNDTIVGAKFNDEKTKHIVTHYISRDALNKTITV
                     LSKIIEVFEEHFDRAITCEMFSDSSTFASINFSEYGISKSKFQQYLRDSCFIENFGVE
                     HTTVSDIQNSIVTFYDVHTDIFRLLNKLNIDISEANIMNQTTVLLDEKNIELLLSKAP
                     YLVSMIVEDFSKLSVDDFSLDNNDLKINLPSPMNEPVVGVIDTLFDKRVYFNEWVEYH
                     DFVSPDISKDSQDYKHGTAVTSLIVDGANLNPNLDDGCGNFRVRHFGVSLQSGFNSFT
                     IIKQIKEIVSQNADIKVWNLSLGSNDEIRENFISAEGALLDEIQFENDVIFIIAGTNA
                     SVINGKRKRIGAPADSLNSIIVNSVDFNNQSVSYSREGIVLSFFVKPDVSYYGGGNGD
                     FINVCEPLGLGRVAGTSFAAPFIARKMAYLIHIMGLSREEAKALLIDAAIPWNDKKTF
                     TDLSLIGNGIVPIKMDDILSTPDDEIKFIVSDISRAYDTYNYDFPVPISSESYPYVAK
                     ATMCYFPNCSRKQGVDYTNTEMQLTFGRLKSDGIKSINKDNQHAEDTPGYVRENAARN
                     IFRKWDNVKHIGESFTSRKRAKAILNPSNPQWGMSIKTIERLKSGDGQGVRFGVVVTL
                     KELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSLNEEIEFE"
     gene            18309..19325
                     /locus_tag="VQM60_000570"
     CDS             18309..19325
                     /locus_tag="VQM60_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET1080463.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYRDFYEFQNKVYISRTENEEKYSN
                     FIYESLFSDKVNDYENWSDFELSIGKLTKDNDLISSSIEIKEKFIDDFSEVVDDLREY
                     LRIQQEKNLEKGNAIDFISTLDDMRTSLPVINQPAIDKKYNENPHQDDIVNIVTLNYT
                     HVIDKLYNGSAKSFRNQLRANLYNFYIEPPIHAHGTVDVCTVLGVSDEIQISNSLEEQ
                     KESLIKSLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDRYIWSQIAERSISGSV
                     PVIIYHYVPHFDPGNPIRAKRLYRNVEDKFIQNSGIDLELEKKLRDNLIVVIGKTIFD
                     LIER"
     gene            19340..20290
                     /locus_tag="VQM60_000571"
     CDS             19340..20290
                     /locus_tag="VQM60_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET1080464.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSLDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            20292..21035
                     /locus_tag="VQM60_000572"
     CDS             20292..21035
                     /locus_tag="VQM60_000572"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET1080465.1"
                     /translation="MQQYFVKGSAISPVTIEDKEISKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            21046..22842
                     /gene="pepF"
                     /locus_tag="VQM60_000573"
     CDS             21046..22842
                     /gene="pepF"
                     /locus_tag="VQM60_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET1080466.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            23088..23642
                     /locus_tag="VQM60_000574"
                     /pseudo
     CDS             23088..23642
                     /locus_tag="VQM60_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
     gene            23753..24421
                     /locus_tag="VQM60_000575"
     CDS             23753..24421
                     /locus_tag="VQM60_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET1080467.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGYLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         7821 a         4042 c         5080 g         7588 t
ORIGIN      
        1 aaagaccaat gattttttgg ataactttat cttcgtaagt aagttcccct ttgatttcgt
       61 gagctacaac agtagcatct ttcttttcta cgttagtgtt tgtgtttttt tcgtttgaca
      121 tatgaaactc ctttaattaa attagtaatt tttatttttt tatttatcta tatagttttt
      181 ttcgatatat aatccagctg caactcccag tgctcctaaa atcaatacaa atattgtttt
      241 gaagaagcca aaggagacaa tcaaacaagc cagaaatacg cctatgagac cagcgataat
      301 tggatatcga tattgtttaa gccattccat tttttacttc cttacttcac acgactaaca
      361 gtctttttgt tttgaggttt tggttggtaa ttttttactg caacttcaag ttttacttga
      421 cgctcaatac caaaaaactg cttcaatcca ttagttattt cattttgaat tatttggcat
      481 ctgtcagcga tgttgtctga aggaagaatt ttaccttcta catgaacgaa acatttattt
      541 tttcgtaaat ttacatgaac agttgggttc ttgatcaatc tatgatcact caccaaactt
      601 ctaacaaaac cttcgattgc cgaattcttt aattttaatg tatcgttttt agtttcaagt
      661 tggatttcca agtaacgttt aggataaaac atcacaacta aaatggataa taaaactaaa
      721 gttgatagaa ccactgtccc ccaaaagaca tatctagcaa gatagaattc aactacggag
      781 ttctgtctcc agctaagtag atgaatacct agatcactaa cttgatgata atctatcaaa
      841 acaggaagga aaattgtcaa gattaaaata cagaaaataa tgaaacatat tttctttgct
      901 tttgacatat acaatccttt cgatttaaca tttaaactat ttttcccgtc attgatttaa
      961 aaatagtttg ctaactttac tggcgaaaag tttactttct acctgtaaag aatgacacta
     1021 cagtaatcac aatcgctgca cctacaatag atgggagcaa agccattcca gcgattgatg
     1081 gaccccaact acctaaaaga gattgtcctg catatgcccc gactaaacca gcgaatacat
     1141 ttgcgatgat tcccatagaa ctactttttt agtgatttta cctgccaaga gaccaataag
     1201 acctccagca ataattgatc acaacactgc ctaccctcct tttttatttt aatatcaaaa
     1261 aatcaatttc tttgattaat actattatat aactttaata tagaaaagtc aattatttcg
     1321 attgttatta ttatataatt ttaaaaaatc aaattcttta attgttgcta ttatataatt
     1381 tttaaaatta aaaatataat ctttagactt gaatttgatt ctgtattcac gccctcaata
     1441 gacggtaaaa taagaaaatt gtttatatat tgtctccgta gtgttattat acgaaataaa
     1501 agattttagg aaaagtcgtt atatcatgct atacctattc tttgtggtat aattgcaaga
     1561 ggtttaatcc gataatttat aaagagagaa agatattcat gagagattta ttatctaaaa
     1621 aaagtcatag gcaattagaa ttattagaat tattatttga acataaacgt tggtttcatc
     1681 gttctgaact agcagagtta ttaaattgta cagaacgtgc agtcaaagat gatctatccc
     1741 atgttaaatc tgcttttcct gacttgattt ttcattcttc tactaatggt atacgcatta
     1801 ttaataccga tgatagtgat attgaaatgg tttaccatca tttctttaaa cattcaactc
     1861 atttttcgat tttagaattc atcttcttta atgaaggttg tcaagctgag agtatttgta
     1921 aagaatttta tatcagttca tcttcgctct atcgtattat tagccaaatc aataaagtga
     1981 ttaaaaggca atttcaattt gaagtcagtc tgacccctgt tcaaatcatt ggaaatgaga
     2041 gagacattcg ttactttttt gcacaatatt tttcagaaaa atattatttc ctagaatggc
     2101 catttgaaaa tttttcatca gagccactat ctcaattgtt agaattggtt tataaggaaa
     2161 caagctttcc aatgaatttg tcaacgcata gaatgctaaa gttgctccta gttacgaacc
     2221 tatatagaat aaagtttggt cattttatgg aagtagataa agattctttt aacgaccaaa
     2281 gtttggattt tttaatgcag gcagaaggaa tagaaggtgt tgctcagagt tttgaatcag
     2341 aatacaatat ctctttagat gaagaagttg tttgccaatt atttgtgtct tactttcaaa
     2401 aaatgttttt catagatgaa agtctcttta tgaaatgcgt aaaaaaggat agttatgttg
     2461 aaaaatctta ccatctattg agtgatttta ttgatcagat ttcagtcaag tatcagattg
     2521 aaatggagaa taaggataat ctgatttggc atctgcataa taccgcacat ctgtatcgtc
     2581 aagagttgtt tactgagttt attttatttg atcaaaaagg gaatacaatc aggaactttc
     2641 aaaatatttt tccaaaattt gtttcagata taaaaaaaga gctttctcac tatttagaga
     2701 ctcttgaggt ttgttctagt tcaatgatgg taaatcatct atcttatacc ttcatcactc
     2761 ataccaaaca tttggtgatt aatttgctac aaaatcagcc taaactaaag gttttagtta
     2821 tgagtaattt tgatcagtat catgcaaaat tcgttgcaga gacactttct tattactgta
     2881 gcaataattt tgaactggaa gtttggactg aattagaatt atcaaaggaa tctttagaag
     2941 attcacctta tgatatcatt atttctaatt ttattattcc tcctattgaa aataagagac
     3001 tcatctattc aaataatata aacacggtct cactcatata cttgttaaat gccatgatgt
     3061 ttattcgatt agatgagtaa ccaaaaataa caaaaaaacg ctatttcgcg tttgaactgt
     3121 acctcaaaag ttagacagaa aaaatctaat ttttggggtg tttttgttaa gaaaagacct
     3181 tcaatcattt aagcaaaatg tacatctaga tttatagccc tctaaaactc ttgctttttt
     3241 tgtagaagat acgaaaaaag ccataaaacc aaggcttttc ctgttttatg tagatgttta
     3301 cacatgaaaa ttgaagtatt ttcaaaagct aattggagtg attttattca tgatgggata
     3361 aatgatctgg ccaaaaatga ttgagaaata gttgcttaaa aagcatttgc atgatactat
     3421 atattgaaag gaggtgataa tatggtagaa caaagaaaat caattaccat gaaagatgtt
     3481 gctttagaag caggagttag tgttggaact gtttcacgtg taattaataa agaaaaaggc
     3541 attaaagaag taactttgaa aaaagtggaa caagcgatta aaactttgaa ttacattcca
     3601 gattactacg ctagaggaat gaaaaaaaat cgaacagaaa cgattgcaat cattgtacca
     3661 agtatctggc atcccttctt ttcagaattt gctatgcatg tggaaaatga agtctataag
     3721 agaaataaca aattactctt atgttctatc aatggtacaa atagagagca agactatctg
     3781 gagatgttgc gtcataataa agttgatgga gtggttgcca ttacctatag tccaattgaa
     3841 cattacttga cgtcaggaat tccctttgtt agtattgacc gcacatactc agatattgcc
     3901 attccttgtg tttcatcaga taatgatgca ggtggaagag aagcagctaa acaattaatt
     3961 agtaaaggat gtcagcattt agcctttgtg ggcggacata atacaaccat taatgaaacc
     4021 aaacgtcgta gaatttcatt tgaaaagtat gtccaagagc ataaaatacc tcttagtatt
     4081 tttgatttgg atgagacagt tactgactat tatggaaagc tagagagttt cttggaagaa
     4141 aattcttcta tagatggaat ttttacaatc aacgatttta cagctttgga tgtgattgaa
     4201 atactaaaaa aaaatggcaa acgtattcct catgatgtcc aaattattgg atacgatgga
     4261 attaaaatgg ctggagatag agattatcta ctttcaacta ttgaacaacc attggaagaa
     4321 atggcaaaag aagcggttcg tattttgttt gatataattg atggaaagac tgttaatttg
     4381 cagacaatcc taccagtaaa atttgttgaa ggaaaaacaa caaaaaatga aaataagtct
     4441 tgacagaaag cgctatcaat gatagaatga attcagataa aaagatttat ttttaaaaca
     4501 aaaatgaaac gtttcaaaaa aagaaataaa aagacagcgc caagcgctat cttttataga
     4561 aaaaatgaaa cgtttcaaaa aaaggaggtt gctatgaata gcaaagcgaa gcaagtttct
     4621 ctttgggaaa gaatcaagaa acaaaaactc ttgttattga tgactgtccc cggtttagtt
     4681 ttaaccttta tctttaaata catccctatg tatggggttt taatcgcatt taaagattac
     4741 aatcctttaa aaggaatttt agggagtgat tggattggtt tttctgagtt tacaaaattc
     4801 atatcctctc ccaactttgg tatcttgtta gccaacacat taaaattaag tatctatggt
     4861 ttattgcttg gctttttacc accaatcatt cttgcgatta tgctcaatca actcttgagt
     4921 gaaaaagtca aaaaacgaat tcagctcatt ttatacgcac caaactttat ctcagtcgtt
     4981 gttattgtcg gtatgatttt cctcttcttt tcagtgggag gaccaatcaa caattttctt
     5041 tctatgtttg gaatgaaggc tgacttcttg acaaatccag acttctttag acctttatac
     5101 atctttagtg gtatctggca aggaatgggc tgggcttcaa cgctctacac ggcaacattg
     5161 gtaaatgtag atccagcctt agtagaagca gctcgactgg atggagccaa tatcttccaa
     5221 cgaatctggc acattgatat tccagctctt aagcctatta tggttatcca atttgtttta
     5281 gctgcaggtg gaattatgaa tgtcggatat gaaaaagcat tcttgatgca gacatcgtta
     5341 aatttgccaa cttctgaaat tatctcgaca tatgtctata aagttggtct tgtatcagga
     5401 gactattctt actcaacagc ggttggtttg tttaatgcag tgattaacgt agtattgctt
     5461 gttgcagtta accaaatcgt taaacgcatg aataatggtg aaggaattta aggaggaaag
     5521 tatgaaaaat tcgattatgg atacaaaatt tgatagacgt atcttactct taaataaaat
     5581 cattattgtc tttatcgttt tgatgacttt gcttccttta ctttatatcg tcgtagcatc
     5641 ctttatggat cctaaggttc tggttagtag agggattagc tttaatccag ccgattggac
     5701 tgtagaaggt taccagcgtg tattcagtga ccaatctatt ctaagaggtt ttatcaattc
     5761 tctactatac tcttttggat ttgcagcttt aacagtattg ctatctgtgt ttacagctta
     5821 tcctctttct aagaaagact tggttggacg tcgttggatt aactacttct tgattgtgac
     5881 tatgttcttt ggtggtggtt tagtcccaac ttacttgctc gtaaaagaat tgggaatgct
     5941 caatactcca tgggctatca ttgttccagg tgctgtcaat gtttggaata ttattcttgc
     6001 tagggcctat ttccaaggat tgcctgaaga attagttgaa gctgctgtca ttgatggtgc
     6061 aaatgattta cagattttct tcaaaatcat gcttcctctt gcaaaaccaa ttatgtttgt
     6121 tctcttcctc tatgcttttg taggacagtg gaactcatac tttgatgcaa tgatttatat
     6181 caaggatcca aacttggaac cattgcaact tgtacttcgt aaaattctca ttcagagcca
     6241 accaggtcaa gacatgattg gagcacaagc ggctatgaat gaaatgaaac gtttagctga
     6301 attgattaaa tacgcaacta ttgtcatttc cagcttgcca ttgattgtta tgtatccatt
     6361 cttccaaaaa tactttgata aaggaattat ggctggttca cttaaaggat aaaaaaagaa
     6421 aaaataaaag gagttttctc atgaaattca aaacattctc aaaatcagca gttttgttga
     6481 cagctagttt agcagtactt gcagcctgtg gctcaaaaaa tacagcttca agtccagatt
     6541 ataagttgga aggtgtaaca ttcccgcttc aagaaaagaa aacattgaag tttatgacag
     6601 ccagttcacc gttatctcct aaagacccaa atgaaaagtt aattttacaa cgtttggaga
     6661 aggaaactgg cgttcatatt gactggacca actaccaatc cgactttgca gaaaaacgta
     6721 acttggatat ttctagtggt gatttaccag atgctatcca caacgacgga gcttcagatg
     6781 tggacttgat gaactgggct aaaaaaggtg ttattattcc agttgaagat ttgattgata
     6841 aatacatgcc aaatcttaag aaaattttgg atgagaaacc agagtacaag gccttgatga
     6901 cagcacctga tgggcacatt tactcattcc catggattga agagcttgga gatggtaaag
     6961 agtctattca cagcgtcaac gatatggctt ggattaacaa agattggctt aagaaacttg
     7021 gtcttgaaat gccaaaaact actgatgatt tgattaaagt cctagaagct ttcaaaaacg
     7081 gggatccaaa tggaaatgga gaggctgatg aaattccatt tacatttatt agtggtaacg
     7141 gaaacgaaga ttttaaattc ctatttgctg catttgatat aggggataac gatgatcatt
     7201 tagtagtagg aaatgatggc aaagttgact tcacagcaga taacgataac tataaagaag
     7261 gtgtcaaatt tatccgtcaa ttgcaagaaa aaggcttgat tgataaagaa gctttcgaac
     7321 atgattggaa tagttacatt gctaaaggtc atgatcagaa atttggtgtt tactttacat
     7381 gggataagaa taatgttact ggaagtaacg aaagttatga tgttttacca gtacttgctg
     7441 gaccaagtgg tcaaaaacac gtagctcgta caaacggtat gggatttgca cgtgacaaga
     7501 tggttattac cagtgtaaac aaaaacctag aattgacagc taaatggatt gatgcacaat
     7561 acgctccact ccaatctgtg caaaataact ggggaactta tggagatgac aaacaacaaa
     7621 acatctttga attggatcaa gcgtcaaata gtctaaaaca cttaccacta aacggaactg
     7681 caccagcaga acttcgtcaa aagactgaag taggaggacc actagctatt ctagattcat
     7741 actatggtaa agtgacaacc atgccagatg atgccaaatg gcgtttggat cttatcaaag
     7801 aatattatgt tccttacatg agcaatgaca ataactatcc aagagtcttt atgacacagg
     7861 aagatttgga caagattgcc catatcgagg cagatatgaa tgactatatc taccgtaaac
     7921 gtgctgaatg gattgtaaat ggcaatattg atactgagtg ggatgattac aagaaagaac
     7981 ttgaaaaata cggactttct gattacctcg ctattaaaca aaaatactac gaccaatacc
     8041 aagcaaacaa aaactagagg ttgattatgg gagataagaa atacacagta gaaaaagcca
     8101 atcgttttat agcagaaaat aaacatctcg ttaatactca atataagcct gaagaacatt
     8161 tttcagctga gattggttgg atcaatgatc caaatggatt tgtctatttt cgtggagaat
     8221 accatctctt ttatcaattc tatccatatg atagtgtttg gggacctatg cactggggac
     8281 atgctaaaag taaggacttg gtgacttggg agcacttgcc agtggcactt gctcctgacc
     8341 aagattatga ccgaaatggt tgtttctcag gctctgccat tgtcaaggat gatcgcctct
     8401 ggctcatgta cactgggcat atcgaagaaa aaaccggtgt ccgccaagtg caaaatatgg
     8461 cattttcaga tgacgggatt cactttgaaa agatttccca aaatccagtt gtaactggaa
     8521 cagacttgcc agatgagttg attgctgctg atttccgtga tccaaaactc tttgaaaaag
     8581 atggacgcta ttactccgta gtagctgtca aacacatgga taatgtgggc tgtatcgttc
     8641 tactagggtc cgataaccta gtagaatggc agttcgaatc catcttttta aaagggggag
     8701 aacaccaagg ctttatgtgg gaatgcccag attacttcga gttagatggg aaagattgcc
     8761 ttattatgtc acccatgcgt tatcagcgtg agggagactc atatcataac atcaactcat
     8821 cgcttttgtt cacgggtaag gtagattgga gagaaaaacg ttttatccca gaatcagttc
     8881 aagaaattga tcatggccaa gacttctatg cgcctcaaac attgttggac gatcaaaatc
     8941 gtcgtatcct gattgcttgg atgcagacat gggggcgtac ccttccaacc catgaccaag
     9001 aacacaaatg ggcatgtgcc atgactttac ctagaattct aagattggaa gatggcaaac
     9061 taagacaatt ccctgttaaa aaaggccaat atcaaatcca aatagataaa gattgtcatt
     9121 accacttagg aaatgatata gattatcttg aatttggtta tgacagtaat gcgcagcaag
     9181 tttacattga tcgtagccat cttattcaaa aaattctagg tgaagaagaa caggacacta
     9241 gtcgacggta tgtagatatt gaagctaaag aattggaagt tgttctagat aaaaattcca
     9301 tcgagatttt tgtcaatcaa ggtgaagcaa gcttgactgc aacttattac ttaacggtgc
     9361 cagctgagct atcacgaatt gattaaaaat taagttattt ctcctaaaga aaaagttctc
     9421 tttctaaaat agtggaaaga ggactttttg tgttttgggt atataagctt agtttatggt
     9481 atttgtaaaa ttggtgttgg attatgattt aagctagttt tctaaagaat ttgaaaaaaa
     9541 ttttatttaa gcaaaaaaag ccctatatta taagactttt cctgttgtat ttagatgccc
     9601 cctacaggga tcgaacctgt gacccacgga ttaagagtcc gctgctctgc cagctgagct
     9661 aaggaggcaa agaaaaaagc tgtattggtg ccgaaacttc acggtttgta ttgaacccgc
     9721 gcaattaagc aggtgggcaa ctcgctctaa ctgaagctgt ttccgtgtga gacggcctac
     9781 atgctgttag aagacttttg tttccctaat aatacaaaaa atagtcggtc aacacttaag
     9841 tgtgaagtcg tacaccacag cgtttctata cttatatgat accacttttt caaaaaaaat
     9901 caagaggaaa gtgcaatttt ttgaaaagga tttcagattt ttcgcaaacg gtcgatagct
     9961 tcctttcttt gagccaaagc ccgttcatat ttaccagtat cttctgcttg gaaatagtga
    10021 tgattacgaa ttttttctgg cagatagtct tgcttgaccc aatttccagg atagttgtgt
    10081 ggatagagat agtcttgggc attccccagt tccttgcttc cactgtagtg cccatcacgt
    10141 aggtgtcgcg gaataggcaa gtgccctgat gttttgaggt cagtaagtgc cttatccata
    10201 gctacatagg ctgagttgga ttttggagaa agggccaaat caatcacgac attggcaatg
    10261 agaatgcggg cttctgggaa cccaatcttc tgggcagcat ccagagcagt cacggtatga
    10321 atctgggcct caggattggc taagccgata tcttcatagg cgataacagt caagcgacga
    10381 gcgagactag gcagatcccc agcctcaatc aagcgggcag catagtggag actggcatcc
    10441 acatctgagc cacggataga cttttgcagg gcagatagaa catcatagtg accgtctcca
    10501 tccttgtcca tagtgatgta gcttctctga agactatttt ccatgatgtc taaggtgatg
    10561 tggcgaatgc cctcgtcatt ctcaggggta gagagaacag ccaagtccag tgagttaaag
    10621 gcagagcgaa ggtctccgtt tgtagaggtt gcgatgaaat ccagcgcatc ctcatctagt
    10681 tctattggaa aatcaaaacc acgttcaggg ttacttagag ctatctgcag ggcctctttg
    10741 acgtcttggt tagacagagg ttccaactca aaaatttgaa cacggctacg aatggcagga
    10801 gtgacggaaa agaaaggatt ttcagttgtt gctccaatca tgatgaccag tccactttcc
    10861 aagagaggca agaggaagtc ttgcttggtc ttatctagtc gatgaatttc atctagcaat
    10921 aggacgagac caccagaaaa tttagcttct tccgagattt cttgcagtcg ctttttacta
    10981 tcaactgtcg cattgaaagt tcgaaaggca tacttggtcg ttccagcgat ggcagaggca
    11041 atactggttt tgccgattcc cggagggcca tatagaatca tggaggacag gcggttggct
    11101 tccaccatgc ggcggatgat ttttccaggt ccgaccagat gctcctgacc gatgacctgg
    11161 tcgatggttt tagggcgcat gcgaagcgcg agattgtctg gcatagtagt cctttctagt
    11221 gtggatttga gtagaatgac tcatctacta tatcttacta tgtccattct agcctattag
    11281 ttgtgatatt tcaatggttt tgtaataatc agggagtgag atagaatctt ttttactatt
    11341 taatttaatg aggagaggta tcagcatgag agatttgatt ttccttaaag ctcaaaatag
    11401 gatagttgag cctaaagaag tggaagtgct gacttgagaa ataatattgc taaatgtcat
    11461 tgtatgaact ataatgaaaa gagaggtgat aagcaatgga aatgatggac caagtcagga
    11521 aattttgatt tttacaaaac aaattcgtca ttggattctt agtgatcaag ttatttcagg
    11581 gaaaagaaaa cttttcttcc gggaagatac tccaaaagag attttagatt tgtacgaaaa
    11641 cattaaatct aaacttgatt ttgcttacca agaagtttat tctaataatg ggttgaaaaa
    11701 atatgaaaaa tagaataatt gatgtttttg aagtagtaaa tcgcatcctt gtaataactg
    11761 ttgaaaatcc tgatttcgaa gacttgagag ttaatcatac tcaatgaaaa tcaaagagca
    11821 aactaggaaa ctagccgcag gctgtacttg agtacggtaa ggcgacgttg acgtggtttg
    11881 aatttgattt tcgaagagta tcagtttgta aaaattggag ataaaaaata tagagtacgc
    11941 agtgttccta tgattcattc aactccgcct caatctgtct tagatcgaga tacttttaca
    12001 attgactata cagatgatga attgctcgat aaagaagcag tttttactac tcattagaaa
    12061 aataggtctg aataaattgg cttcccttag aaccacatct aggggctttt atgtataata
    12121 gaataataag tatcactcct ttacaatacg tgtaacacgt gttataatgg tgttgtcagg
    12181 aggtaaaagc gctatgccta tgacacaaaa agagatggtt aaactcctta cggcccatgg
    12241 ttggataaag actagaggcg gtaaaggctc ccatattaaa atggagaagc aaggggaaag
    12301 acttatcaca atccctcatg gtgagctgaa taagtacact gaaagaggga taagaaagca
    12361 agctgggttg taaaattcag cccctgtttt ttgatatgga ggtagtgaaa tgttagttac
    12421 gtatccagct ttattttatt atgacgacac agacggaaca gaagcgactt attttgtcca
    12481 tttcccagat tttgaatact cagctacaca aggagagggg atttctgagg ctttggctat
    12541 ggggtcggag tggctaggga taactgttgc agatttgata gagagtgatg gagagttacc
    12601 tcagccatca gatatcaata gcttgtcttt aattgataat gatcctttta aagatgatga
    12661 agatttcgtg tcaacctatg accttgataa atcttttatt tctatggtat ctgttgatgt
    12721 atcagaatac ctaggaagtc aggaacccat taaaaagact ttgactatac caaaatgggc
    12781 agataagttg ggacgagaaa tgggacttaa cttttctcag actttgacag acgctattgc
    12841 agataagaaa gttcaagcct aagattaagc gtggtatcgt ggtaatagtc aataaaaaag
    12901 cacgtccact tgtgctagtt actcgcctat tgattttata gatttattag ccctttcgag
    12961 ggctttttat attgattttt atgagaaatg aagaaatcag acttttaaga aaatcactga
    13021 taccaagggt ttaaatgagg taatatggta taattaggac ataaaataat tttgtggtaa
    13081 gatggtagta tctattttag catatttccg agcaatcggg gcgattaaag agtcgcatag
    13141 aaagaggaca aaatggcaac atacggattt ttagatgttt tagaggaaga gttggacaag
    13201 aactttccct ttgactttga gattagctgg gacaaacgca actatgcggt agaagtgagt
    13261 tttctattgg aagcgcaaaa tgctgcaggt gtggagatgg tggatgaaga cggagaggtt
    13321 tcatcagatg acattctttt tgaggaagcg gtccttttct acaatcctgc caagtcaaca
    13381 gtcaatgagg aagactattt gactgttatt ccttacctgc ctaaaaaagg attttcccgt
    13441 gaatttttag cttattttgc gcttttcctt aaagatactg ctgaggttgg gctagatgcc
    13501 ctcatggact ttttggaaga tccagaagca gaggaatttg ttatggaatg gaatcaagaa
    13561 gtctttgaag aaggaaaagt tggcttggaa gagggagaat tttaccctta tccaaaatac
    13621 taggagttgg ttggaggtgt tatgaagaaa attgggactt atttggttta tgtgctagct
    13681 gttgtcttta ttatgctagc ttttgcttgt ggaacaatcg catttgcaga gttggggtat
    13741 tccgcagttt tagcctttac ttttggttat gcctttgctc ttctaagcat gtatttaatc
    13801 tttattcttc atgagctggg tcatgctttt tgtggctact tgacaggcta tcggctggtg
    13861 gcttttggat taggacattt tattttgacc aaaaagtcag gcaggtttca tcttagtaga
    13921 acagccattc tgaaaaatgt tggtgctcaa tacattggtt taaaagagga tgaaagcgat
    13981 caaagaatca tcctgatgct ttcaggaggc ttgatggttc atctcggctt gatattattg
    14041 gcgatattgt ttggattttt gacaagaagc tggtattttg cagggacttg gatttttctt
    14101 aatttatctt tcttcctaaa taacatttta ccagtcgaca tcaccgatgg agcaaaaatt
    14161 tgggaattac tacaacaccc tgaaaatacc aaatacgcct acctggtatt gagacattct
    14221 gcccagacct tgctggctcc tcaagaatat gatttgaaag actttgttca agcagttcct
    14281 gaggatgcac gagggagttt tgcggatggt gtattaggta tgcaaggtga agtttccatt
    14341 ttggaaggga aggaagaagt agctaaacag cagtttcaag cattacttga gaggacaact
    14401 actccgatga tgcagacagt tgcccaacta tccctgcttc atatcgcctt gttggaagga
    14461 gattttgagc aagcggagca atatgccagt attcgacagg tcaaatcgtt tctgtctctc
    14521 aaactgtcca atcttcaagt gatacaagcc tggtatcaat ttaaggtaag gaaagatgtg
    14581 gttcaaactc acaaggctat gaagattgct agacagaaaa tggatagtag tcgaatgttg
    14641 agggacgaga aacgctatta tgaaaacttg ttagcagagc tggaaaagga aatgttagaa
    14701 ggaactgaag atggaactag aaatttctga tttcccaggc tgtaagattg ctttgttttg
    14761 tggggataag cttttgacta tcttacgcga tgataaggca agcattccct gggccaatat
    14821 gtgggaactg ccaggtgggg gaaagagtgt cagggaaaat ctgatatagt tttgcattaa
    14881 aagaatatcc tttcaaggtt attctttttt aattttgtcc caaaataatt gactttgtcc
    14941 cgaaaatata gtattatttg tataaggagg aatgaatcat gaaaaaatca gatgttcttg
    15001 atttaataaa gtatcattat gagggcagag agacagaatt tagaaatcaa tccatagcaa
    15061 ttgctcggaa cttcaataaa catggtgata cacaaatagc acaatatatt atggggttaa
    15121 tgtctcaaag tgatagattc atgccacaaa tagaaaatcc tagtgagtat ttgactcctg
    15181 ctaaactaga tattggtcct ctcccattgc ctctttcaat tatgaatgat ttaaaaggaa
    15241 taataaacgc tgtcaatcat catattggaa taaataaatt tttatttgtg ggatctccag
    15301 gtactggaaa aactgaaagt gtaaagcagg tggctagact tattggcaag gaattacttg
    15361 tagtagattt cagtcactta gtagatagta agctagggca aacagtcaaa aacttagcaa
    15421 ctctttttaa tgaaatcaat aatcttcctt tcaagcaaaa ttatatcatc ctatttgatg
    15481 aaattgattc catagtgttg gatagggtta atcagaatga tttaagagaa atgggacgag
    15541 tgacttctgc ctttttaaag gagttggata ggctgtcacc agaaattgta ttaattgcaa
    15601 caacaaatct ttttgaaaat cttgataaag cagttacgag aagattcgat gccataattg
    15661 actttgaccg ttatactgat gaagataaag tagaagtagc tactattatt ttaaatgagt
    15721 tattaaagca atttaagaat gtagctagag atttaaaatt atttaaaaaa attatcaata
    15781 gtgccaatgt aataccgaat cctggtgatt tgagaaactc aataagaacg tcattggcat
    15841 ttagtgaccc atcagatccg catgattacc aaaaacgcct gctaagaagt ttgcataatg
    15901 gtagaaattt atctatttct aaattatcga agctaggttt tactgttaga gaaattgaga
    15961 ttttgacagg tatttctaag agtagtgtat cacgagagtt aagcgaggat taaaatgaat
    16021 acattattga cattacgagg taaaagtttt acacaaaaat caagaaataa tggtatggga
    16081 ccaataacta tacccaaaaa aacaataatt acattggaac atttgaagta tcttcatttt
    16141 tcattagaag agacgaaaac atattgggag aaaaataaca ttatcgatgg tattttgatc
    16201 agtatttact ataatcgaat tgttgccaaa agtaaccgta ttaatggcta tttcaatgta
    16261 ggaggaggga atccattccc taatgatact attgtgggag caaaattcaa tgatgagaag
    16321 acaaaacata ttgttactca ttatatttct agagatgctc ttaataaaac aataaccgtt
    16381 ttaagtaaga taattgaagt ttttgaggaa cattttgata gagcgataac atgtgagatg
    16441 ttttctgatt catcaacgtt tgcttctata aatttttcgg agtatggtat ttcaaaaagc
    16501 aaatttcaac agtatttgag agattcttgc tttattgaga attttggagt tgagcacact
    16561 actgtttctg atattcaaaa ttctatcgtg acattttatg atgtccatac agacattttt
    16621 agattactga ataaattaaa tattgatatt tcagaagcta atatcatgaa tcagaccacg
    16681 gtcttgcttg atgaaaaaaa tattgaattg ctactatcaa aagcgcccta tcttgtctcc
    16741 atgattgtgg aagatttttc taagttatct gtagatgatt tttctttaga taataatgat
    16801 ttaaaaataa atcttccatc tccaatgaac gaaccagttg ttggcgttat tgatacctta
    16861 tttgacaaaa gagtttactt taatgagtgg gtagaatatc atgattttgt ctcacctgat
    16921 attagcaaag atagccaaga ttacaagcat gggacagctg tgacttcttt aatcgttgat
    16981 ggagccaatt tgaaccctaa tctggatgat ggttgtggaa attttagagt gaggcatttt
    17041 ggtgtgtcac tacaaagtgg atttaattca tttacaatca taaaacagat aaaagagatt
    17101 gtttctcaga atgctgatat taaggtttgg aatctttctt tagggtccaa tgatgaaata
    17161 agagaaaatt ttatctctgc agagggagca ttattagacg aaattcaatt tgagaatgat
    17221 gtaattttta tcatagctgg gacaaatgct tcagtaataa atgggaaacg caaaagaata
    17281 ggtgctcctg ctgattcact taattctata atagtaaatt ctgttgattt taataatcaa
    17341 tcagtgtcat attcacgaga gggtattgtt ttatcattct ttgttaaacc agatgtgtca
    17401 tattatggag gaggaaatgg tgactttata aatgtttgtg aacctctagg attaggtcgt
    17461 gtagctggaa caagttttgc ggcacctttt attgcaagga aaatggccta tctcattcat
    17521 attatgggac ttagccgaga agaagcgaag gctttgctaa ttgatgcagc aataccctgg
    17581 aatgataaga aaacattcac tgatttatct ttgataggaa atggtattgt tccaataaaa
    17641 atggatgata ttttatctac acctgatgat gaaattaaat ttattgtgtc agatatctct
    17701 agggcttatg atacctataa ttatgatttt ccagtaccta tttctagtga gagttatcca
    17761 tatgtagcta aagcaacaat gtgttatttc cctaactgct caagaaaaca aggggttgat
    17821 tatacaaata ctgagatgca gcttactttt ggtaggttaa agtcagacgg tatcaaatcg
    17881 ataaataaag acaaccagca tgccgaagat accccaggat atgtgagaga aaacgctgct
    17941 agaaatatat ttaggaaatg ggataatgtc aagcatattg gagagtcgtt tacttctcga
    18001 aagagagcta aagcaatttt aaatccatct aatcctcagt gggggatgag tattaaaaca
    18061 atcgaacgtt tgaaaagcgg tgatggacaa ggagtacgtt ttggtgtagt agttacatta
    18121 aaagaattaa atggggttaa tcgtattgaa gattttattc agcaagctga attacgcggt
    18181 tggctagtta atagattgca agtagaagct caagtagacc tctttaatag tttaaatgaa
    18241 gaaattgaat ttgaatagtg gatattttat ccttatttta gatcttagga acacaagagg
    18301 gcaaagatat gaatattact tatatagttg gaaatggttt ggatttgcaa tatggtttga
    18361 aaacaagata tagggatttc tacgaattcc aaaataaggt ttatataagt aggacagaga
    18421 acgaggaaaa atattcaaat ttcatttatg agtctctatt ttctgataaa gtaaatgatt
    18481 atgaaaattg gtcagatttt gaattgtcaa ttggtaaatt gacaaaagat aacgacctca
    18541 tctcctcaag tatagaaata aaggagaaat ttatagatga ttttagtgaa gtagttgatg
    18601 atttaagaga atatctacgc atacaacaag aaaaaaatct tgagaaaggt aatgcaattg
    18661 actttataag cactctagat gatatgagga cctctctccc tgtaattaat caacctgcta
    18721 tcgataaaaa atacaatgag aatccacatc aggacgacat tgtaaatata gtaacactta
    18781 attatacaca tgtaatcgat aaattatata atgggtcagc aaaatcattc aggaatcaat
    18841 tacgagctaa tctttataat ttttatattg agccaccaat tcatgcacat gggacggtgg
    18901 atgtctgcac ggttttaggg gttagtgatg aaatacaaat ttcaaatagt ttagaagaac
    18961 aaaaggaatc cctaataaaa agtttagttt taaaaaacta tagagaaaat atggatgtaa
    19021 agaatagtga tattataaaa aattctgaca tcattattct ctatggggtt tctttgggag
    19081 aaactgatag gtatatctgg agtcagattg ctgaacgatc aatcagcggt tctgttcctg
    19141 tcataattta ccactatgta cctcattttg atccggggaa tcccattaga gcaaaacgcc
    19201 tatatagaaa tgtagaagat aaattcatac agaatagtgg tatagactta gagttagaga
    19261 aaaaattaag agacaacctt attgtcgtta ttggtaagac tatttttgat ctaatagaga
    19321 ggtaatttta aaaaacaaaa tggaaacatg gcaagagtta aaagttacag tgaagcgtga
    19381 gggggaggag ttggtttcca atctcttgat tgagctggga gcgcaaggtg ttgcgattga
    19441 agacagtctg gactatgtgg gaaatgttga ccgctttggc gagattttcc cagaggtgga
    19501 gcagcaagaa gaaatcgtag tgaccgccta ctaccctgac acggtggatg tgacagtggt
    19561 tgaggcggat ttgcaggctc gtctagcaga attgacagat tttatggatt tgggagagtt
    19621 aaaaataggt acgactgcct tggctgagga agactgggca gacaactgga agaaatacta
    19681 tgagccagct cgtatcactc atgatttgac cattgttccc tcttggacag actatgaggc
    19741 gactgcggga gaaaaaatta tcaagctgga tcctggcatg gcttttggta ctggaaccca
    19801 cccaactacc aagatgagcc tttttgcctt ggaacaggtt cttcgtggtg gcgaaacggt
    19861 gctagatgtg gggactggtt caggggttct ctctattgcc agctcgcttc tgggggctaa
    19921 agaaattttc gcctatgacc tggatgatgt ggcggttcgt gtggctcagg aaaatattga
    19981 gctcaaccct ggcatggaaa atatccatgt agcggctgga gatttgctta agggtgtgga
    20041 aattgaggca gatgtgattg tggctaatat cttggcggat atcctcattc atctaataga
    20101 cgatgcctat cgtttggtca aggacgaagg ctacctgatc atgagtggca ttatcaagga
    20161 taagtgggac atggtgcgtg agtcggctga atcggctgga tttttcctcg aaacccacat
    20221 gattcaaggg gagtggaatg cctgtgtctt taagaagacc aaggatattt caggggtgat
    20281 tggaggctag catgcagcag tattttgtaa aaggcagtgc tatctctcct gtcactatcg
    20341 aggacaagga aatcagtaag catatgtttc aggttatgcg cttgaaagaa gaggatgagg
    20401 ttaccttagt ctttgatgat ggcatcaagc gcttggcgcg cgtgctggat atggaaaatc
    20461 gtcagtttga gttggtccaa gaattaaatg acaatgtgga actaccagtc caagtgacca
    20521 tcgcatctgg atttcccaag ggagacaagc tggagttcat cgctcaaaaa gtaaccgaac
    20581 tgggtgctag ccaaatctgg gcctttcctg cagactggtc agttgccaag tgggatggca
    20641 agaaattggg taaaaaagtt gaaaaactag aaaaaattgc ccttggagca gccgagcaaa
    20701 gcaagcgtaa tattgtacca agtattaaac ttttcgagaa aaaagcagat tttctagctc
    20761 agctggacca gtttgactct atcatagtag cctatgaaga atcagctaaa gaaggagaag
    20821 ccgctgcgct tctgcaagca gtcactggtc ttgaaacagg agccaaactg ctctttatct
    20881 ttggtccaga aggtggtctc tcacctgcag aaatcgaaag ttttgaagct aaaggagcag
    20941 ttttggcagg cctaggtcct cgtattttgc gagcagaaac agcaccgctt tacgccttat
    21001 cagcccttag tgttttagta gaattagaga aataagagga agaaaatgga acaaaaacac
    21061 cgttcagaat ttccagagaa ggaactctgg gacttaacag ccctatacca agaccgtgag
    21121 gatttcttgc gtgcaatcga gaaagctcgt gaagacatca accaatttag ccgtgattac
    21181 aagggcaatc ttcacacttt tgaggatttc gagaaggcct ttgcggaatt ggaacagatc
    21241 tacattcaga tgagccatat tggcaactat gcttttatgc ctcagacgac ggactatagc
    21301 aatgacgaat ttgccaatat tgcccaagct gggatggaat ttgaaacaga tgccagcgta
    21361 gccttgacct tctttgacga tgccttggtg gtagcagacg aggaagtctt ggaccgtttg
    21421 ggtgaattgc ctcacttgac ggcagctatt cgtcaggcca aaatcaaaaa agcccactat
    21481 ctaggggctg atgtggagaa ggccttgacc aatctcggtg aagttttcta tagtccgcag
    21541 gacatttata ctaagatgcg agctggggat tttgaaatgg ctgactttga agcccatggc
    21601 aagacctata aaaacagctt tgtcacctat gagaatttct accaaaatca cgaggatgct
    21661 gaggttcgtg agaaatcctt ccgttccttc tcagagggac ttcgtaagca ccaaaatacg
    21721 gctgcagcag cctatctggc tcaggtcaag tctgaaaaac tcttggctga tatgaaggga
    21781 tacgactctg tctttgatta tcttctagct gaacaagaag tggaccgtgt catgtttgac
    21841 cgccagattg acctcatcat gaaggacttt gcaccagtcg ctcagagata cctcaagcat
    21901 gttgccaagg taaatggtct tgaaaagatg acctttgcag attggaaatt ggacttggac
    21961 agcgccctga atcctgaagt gactattgac gatgcctatg atttggtcat gaagtcggta
    22021 gaacctttgg ggcaagaata ttgtcaggaa gttgctcgtt accaagaaga gcgctgggtg
    22081 gactttgctg ctaacagtgg caaggattcc ggtggttatg cggcggaccc atatcgcgta
    22141 cacccttatg tactcatgag ctggacaggc cgtttgagcg atgtctatac cttgattcat
    22201 gaaatcgggc attctggtca attcatcttt tcagacaatc atcaaagcta tttcaacgcc
    22261 cacatgtcga cctattatgt tgaagcaccg tcgaccttca atgaattgct actcagtgat
    22321 tacttagaga accagtctaa tgacccacgt caaaaacgct tcgctctggc tcatcgcttg
    22381 acagacacct acttccataa tttcatcacc cacctcttgg aagccgcctt ccagcgtaag
    22441 gtgtatacat tgattgaaga aggggagacc tttggagcaa gcaagctcaa cagcattatg
    22501 aaggaagttt tgacagattt ttggggcgat gccattgaaa tcgacgacga tgcagctttg
    22561 acttggatgc gccaagctca ctactatatg ggcttgtata gttacactta ctcagcagga
    22621 ctagttatct cgactgctgg ttaccttcat cttaaaaact cagaaactgg agctgaagac
    22681 tggctcaatc tcctcaaatc aggtggtagc aagacaccac ttgagtcagc catgattatc
    22741 ggtgcggata tttcaacaga caaaccactc cgtgatacca tccaattctt gtctgacaca
    22801 gttgaccaga ttatcgccta cagtgctgag ttgggggagt aaggggatag aaaaattttt
    22861 tagaatgatg tttatgggct tgttaagcgt cattctattt gtcttagttg tagctatttt
    22921 caaaaatgtc gttgaaggtc gtgatgttaa atttgaactt actgtagaac tggtattgat
    22981 agttatttac gtcattttat atcagattgt atttaattga gaattcaata ttgagtcatt
    23041 taagactgcc tttttgaaca gttgctagaa atgtagaggt attgaccatg tatcaagatt
    23101 tactaagaaa aatagcagaa gaaaaaccaa attataacca ggaagaaatc cagtggttgt
    23161 ttgatcattt gggaaatcct tctccagaaa ttcgtgatga cctttcaaat caaggcttgc
    23221 actatctttc aaaagaaaag gatacaacag gtttctcaag tcagtatggt tgggttcacg
    23281 cttttgcaca tggagccgat ttactgacag aggtggtttg tcatccagac tttcctaaaa
    23341 acagagttca tgaagtattt gatatacttg gccaactatt taaaagaatg tcgattcgct
    23401 ttacagatga tgaggattgg cgtttagcaa gagtgatcta tgaacctatt ttataaggga
    23461 agttggagca agaacaagta gcttcttgga taaaaactgt tgattttccg atagaagaaa
    23521 gggaggattt ttacaaattt tccaacttca gatcctgtct ggtggaagtc tatgtccaac
    23581 ttgaccagag aaatagttta caagatgagt tgaaagaagc tatccagtct tttcaatact
    23641 agagattagc taatagttct atgattttca aaaaatttcc aatcaatttt cttgaaaccc
    23701 tttcctactt ttgatagact agtatctagt ttttgaaaaa aggagaaaga aaatgaaaaa
    23761 atttgttgct gagttaatcg gtacgttcat gcttgtgttc gtcgggacag gagctgttgt
    23821 ttttggaaat ggtcttgatg gccttggtta ccttggaatc gcctttgcct ttggtttggc
    23881 aatcgtggtg gcagcctact caatcggaac tgtttcaggt gctcacttga acccggctgt
    23941 ttcgattgct atgtttgtaa acaaacgttt gtcatcttca gaacttgtaa actacatcct
    24001 tggtcaggtt gttggagctt tcatcgcttc tggcgctgtc ttcttcctct tggctaactc
    24061 aggtatgtca actgctagtc ttggtgaaaa tgccttggca aacggtgtca ctgtctttgg
    24121 tggtttcttg tttgaagtca tcgcaacttt cttgtttgta ttggttatca tgactgtgac
    24181 ttcagaaagc aagggcaatg gcgcgattgc tggtttggta atcggtttgt cattgatggc
    24241 gatgattctt gtcggattga agattactgg actttcagta aacccagctc gtagcttggc
    24301 accagctgtc ttggtaggcg gcgcagccct tcaacaagtt tggattttca tccttgcacc
    24361 aatcgctggt ggagttcttg cagcccttgt tgcaaaaaat ttccttggaa cagaagaata
    24421 attgaaactc aaaaagcctt gctcctcatc ttgaggaaca gggctttttc gtatgatact
    24481 cttcgaaaat ctcttcaaac cacgtcagct tcaccttgtc gtatagatgt t
//
LOCUS       DAVAAL010000018        23001 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVAAL010000018 DAVAAL010000000
VERSION     DAVAAL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23001
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.018"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            58..426
                     /locus_tag="VQM60_000576"
     CDS             58..426
                     /locus_tag="VQM60_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET1080468.1"
                     /translation="MAVGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKAK
                     NMENAKKFIDFIISQEVQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIK
                     NKSDIVKKYNEVFTDIQSKQ"
     gene            439..825
                     /locus_tag="VQM60_000577"
     CDS             439..825
                     /locus_tag="VQM60_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080469.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYAD"
     gene            835..921
                     /locus_tag="VQM60_000578"
                     /pseudo
     CDS             835..921
                     /locus_tag="VQM60_000578"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            907..1530
                     /locus_tag="VQM60_000579"
     CDS             907..1530
                     /locus_tag="VQM60_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080470.1"
                     /translation="MDEPLSNLEAKLRLDMRQTIREIQHEVGITTVYVTHDQEEAMAI
                     SDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDG
                     YALRMLALDQVEEQAIHVSIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASK
                     IQVSEESTFEEDLQKDNRIRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            1520..3155
                     /locus_tag="VQM60_000580"
                     /pseudo
     CDS             1520..3155
                     /locus_tag="VQM60_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(3258..3950)
                     /locus_tag="VQM60_000581"
     CDS             complement(3258..3950)
                     /locus_tag="VQM60_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET1080471.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(4194..5531)
                     /locus_tag="VQM60_000582"
     CDS             complement(4194..5531)
                     /locus_tag="VQM60_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET1080472.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(5541..5807)
                     /locus_tag="VQM60_000583"
     CDS             complement(5541..5807)
                     /locus_tag="VQM60_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET1080473.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(6072..6458)
                     /gene="rplQ"
                     /locus_tag="VQM60_000584"
     CDS             complement(6072..6458)
                     /gene="rplQ"
                     /locus_tag="VQM60_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET1080474.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(6470..7405)
                     /locus_tag="VQM60_000585"
     CDS             complement(6470..7405)
                     /locus_tag="VQM60_000585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET1080475.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(7448..7831)
                     /gene="rpsK"
                     /locus_tag="VQM60_000586"
     CDS             complement(7448..7831)
                     /gene="rpsK"
                     /locus_tag="VQM60_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET1080476.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(7849..8214)
                     /gene="rpsM"
                     /locus_tag="VQM60_000587"
     CDS             complement(7849..8214)
                     /gene="rpsM"
                     /locus_tag="VQM60_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET1080477.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(8232..8348)
                     /gene="rpmJ"
                     /locus_tag="VQM60_000588"
     CDS             complement(8232..8348)
                     /gene="rpmJ"
                     /locus_tag="VQM60_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET1080478.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(8373..8591)
                     /gene="infA"
                     /locus_tag="VQM60_000589"
     CDS             complement(8373..8591)
                     /gene="infA"
                     /locus_tag="VQM60_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET1080479.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(8708..9346)
                     /locus_tag="VQM60_000590"
     CDS             complement(8708..9346)
                     /locus_tag="VQM60_000590"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET1080480.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(9497..10807)
                     /gene="secY"
                     /locus_tag="VQM60_000591"
     CDS             complement(9497..10807)
                     /gene="secY"
                     /locus_tag="VQM60_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET1080481.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(10820..11260)
                     /gene="rplO"
                     /locus_tag="VQM60_000592"
     CDS             complement(10820..11260)
                     /gene="rplO"
                     /locus_tag="VQM60_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET1080482.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(11405..11629)
                     /gene="rpmD"
                     /locus_tag="VQM60_000593"
     CDS             complement(11405..11629)
                     /gene="rpmD"
                     /locus_tag="VQM60_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET1080483.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(11601..12095)
                     /gene="rpsE"
                     /locus_tag="VQM60_000594"
     CDS             complement(11601..12095)
                     /gene="rpsE"
                     /locus_tag="VQM60_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET1080484.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(12113..12469)
                     /gene="rplR"
                     /locus_tag="VQM60_000595"
     CDS             complement(12113..12469)
                     /gene="rplR"
                     /locus_tag="VQM60_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET1080485.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(12553..13089)
                     /gene="rplF"
                     /locus_tag="VQM60_000596"
     CDS             complement(12553..13089)
                     /gene="rplF"
                     /locus_tag="VQM60_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET1080486.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(13281..13679)
                     /gene="rpsH"
                     /locus_tag="VQM60_000597"
     CDS             complement(13281..13679)
                     /gene="rpsH"
                     /locus_tag="VQM60_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET1080487.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(13964..14233)
                     /gene="rpsN"
                     /locus_tag="VQM60_000598"
     CDS             complement(13964..14233)
                     /gene="rpsN"
                     /locus_tag="VQM60_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET1080488.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(14251..14793)
                     /gene="rplE"
                     /locus_tag="VQM60_000599"
     CDS             complement(14251..14793)
                     /gene="rplE"
                     /locus_tag="VQM60_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET1080489.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(14817..15122)
                     /gene="rplX"
                     /locus_tag="VQM60_000600"
     CDS             complement(14817..15122)
                     /gene="rplX"
                     /locus_tag="VQM60_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET1080490.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(15200..15568)
                     /gene="rplN"
                     /locus_tag="VQM60_000601"
     CDS             complement(15200..15568)
                     /gene="rplN"
                     /locus_tag="VQM60_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET1080491.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(15594..15854)
                     /gene="rpsQ"
                     /locus_tag="VQM60_000602"
     CDS             complement(15594..15854)
                     /gene="rpsQ"
                     /locus_tag="VQM60_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET1080492.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(15879..16085)
                     /locus_tag="VQM60_000603"
     CDS             complement(15879..16085)
                     /locus_tag="VQM60_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET1080493.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(16095..16508)
                     /gene="rplP"
                     /locus_tag="VQM60_000604"
     CDS             complement(16095..16508)
                     /gene="rplP"
                     /locus_tag="VQM60_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET1080494.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(16512..17165)
                     /gene="rpsC"
                     /locus_tag="VQM60_000605"
     CDS             complement(16512..17165)
                     /gene="rpsC"
                     /locus_tag="VQM60_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET1080495.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(17178..17522)
                     /gene="rplV"
                     /locus_tag="VQM60_000606"
     CDS             complement(17178..17522)
                     /gene="rplV"
                     /locus_tag="VQM60_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET1080496.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(17534..17815)
                     /gene="rpsS"
                     /locus_tag="VQM60_000607"
     CDS             complement(17534..17815)
                     /gene="rpsS"
                     /locus_tag="VQM60_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET1080497.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(17919..18752)
                     /gene="rplB"
                     /locus_tag="VQM60_000608"
     CDS             complement(17919..18752)
                     /gene="rplB"
                     /locus_tag="VQM60_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET1080498.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(18770..19066)
                     /locus_tag="VQM60_000609"
     CDS             complement(18770..19066)
                     /locus_tag="VQM60_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET1080499.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(19066..19689)
                     /gene="rplD"
                     /locus_tag="VQM60_000610"
     CDS             complement(19066..19689)
                     /gene="rplD"
                     /locus_tag="VQM60_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET1080500.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(19714..20340)
                     /gene="rplC"
                     /locus_tag="VQM60_000611"
     CDS             complement(19714..20340)
                     /gene="rplC"
                     /locus_tag="VQM60_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET1080501.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(20557..20865)
                     /gene="rpsJ"
                     /locus_tag="VQM60_000612"
     CDS             complement(20557..20865)
                     /gene="rpsJ"
                     /locus_tag="VQM60_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET1080502.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(21131..21748)
                     /locus_tag="VQM60_000613"
     CDS             complement(21131..21748)
                     /locus_tag="VQM60_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET1080503.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(21745..22335)
                     /gene="nrdG"
                     /locus_tag="VQM60_000614"
     CDS             complement(21745..22335)
                     /gene="nrdG"
                     /locus_tag="VQM60_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET1080504.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGILYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(22328..22834)
                     /locus_tag="VQM60_000615"
     CDS             complement(22328..22834)
                     /locus_tag="VQM60_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1080505.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
BASE COUNT         6452 a         5117 c         4254 g         7178 t
ORIGIN      
        1 tactataaaa tcggtttaag ttcatctggt gtctataaag cagtcgctga tggagaaatg
       61 gctgttggtc tctcttatga ggatccagca gttaaactct taaatgacgg agctaacatt
      121 aaggtagtct atccaaaaga aggaaccgtc ttcctacctg ctagtgctgc tatcgttaaa
      181 aaagctaaaa atatggaaaa tgccaagaaa tttatcgatt ttattatctc tcaagaagta
      241 caagatacac ttggtacaac cactactaac cgtcctgttc gtaaaaatgc taaaacaagc
      301 gaaaacatga aaccaattga caaaatcaaa acactcactg aagattatga ttatgtcatc
      361 aagaataaat cagatatcgt taagaaatac aacgaagtct ttacagatat ccaatctaaa
      421 cagtaaaaga ggttcactat gagtgagatc gaaattatta acgccaaaaa aatctaccac
      481 gatgtccctg ttattgagaa tttgaacatt acaattccaa aaggaagtct ctttaccctt
      541 cttggagctt caggatgtgg gaaaacgacc cttcttcgta tgattgcagg tttcaacagt
      601 atcaaagatg gagaatttta cttcgatgat acaaaaatca ataatatgga acccagcaaa
      661 cgcaatatcg ggatggtttt ccaaaactac gctattttcc cacatttgac tgtccgagac
      721 aacgttgctt ttggtcttat gcaaaagaag gttccaaaag aagaattgat tcaacagacc
      781 aacaagtatc ttgaactcat gcaaattgct caatatgcgg attgaaagcc cgataaagtc
      841 agtggtggac aacaacaacg tgtcaccttg gcatgcgcct tagcggttaa tccaagtgtt
      901 ctcctcatgg acgagccact tagtaatctg gaggccaaac ttcgcttgga tatgcgtcaa
      961 accatccgag aaatccaaca cgaagtggga attacaactg tttatgtaac ccacgaccaa
     1021 gaagaagcca tggctatttc agaccaaatt gctgttatga aagatggggt gatccaacaa
     1081 atcggccgac caaaagaact ctatcataaa ccagctaatg agtttgtggc aacctttatc
     1141 ggacgcacaa atattatccc tgccaatctt gaaaaacgga gcgacggcgc ttatatcgtc
     1201 ttttcagatg gctatgccct tcgaatgcta gctcttgatc aggttgagga gcaagctatt
     1261 catgtaagca ttcgtcccga agagtttatc aaagatgaat ctggagatat cgaaggaact
     1321 attagcgata gcgtctatct tggactaaat acggagtatt tcattgagac aggttttgcc
     1381 tcaaaaattc aagttagtga agaatcaact tttgaagaag atctacaaaa agacaatcgt
     1441 attcgtctac gaatcaatac gcaaaaatta aacatctttt ctgcagatgg ttcccaaaac
     1501 ctgataaaag gagtcaacca tggaacgtaa aaaactaaat atttggacag cctcctcttt
     1561 cttcatcttt cttacctatc ttgtctttct cgtttatcct atcgttaccg tgctcaagta
     1621 agcacttata catgaaggat aattctcact agctaatttt gtcactttct ttagtaaaac
     1681 ctactactct gagacactag tcaacagttt cagggtttcc attaccgcta ctgtcacttc
     1741 cttagttgta ggaaccctat tagcttatct cttctctatg tatgacttca aggggaagaa
     1801 atttctacaa atattgatta tcattgcttc catgtcaact cctttcgtag gagcctactc
     1861 ctggattctc ttgctgagac gaaatgaggt catcactaaa tttttgacaa atgcccttca
     1921 tcttccagct atcgatattt atggattcaa aggaattgta cttgtcttta cactgcaact
     1981 attcccactg gtatttctat atgttgctgg aacaatgaac agtattgaca attctctact
     2041 tgaagcagct gaaagcatgg ggtccttcgg atttaaacct atcgtaacgg ttgttttacc
     2101 tctcctagtt ccaaccttac tagcagctcc ttgcttgtat ttatgagagc attctcagac
     2161 tttggaacgc ctatgttgat tggcgaagga tatcggactt tccctgtcct gatttatacc
     2221 caatttatta gcgaggttgg aggaaattct gcttttgcaa ttatggcgat tatcattgcc
     2281 ttggcaattt tccttatcca aaaacacatt gcaaaccgct acagtttcag catgaatctg
     2341 ctccatccaa ttgagcctaa aaaaactaca aaaggaaaaa tggctgccat ttatgcaaca
     2401 gtctacggaa ttatctttat ctctgtttta cctcaaatct acttaattta tacctctttc
     2461 ctaaaaacat caggtatggt atttgttaaa ggttattctc caaacagtta caagctagct
     2521 ttcaatcgta tgggatctgc tattttcaat accattcgta tccctttgat tgccttagtt
     2581 ctagttgttc tatttgcgac atttatctcc tacctagccg ttagaaaacg gaatttgttt
     2641 acaaacttaa ttgacagcct cagtatggta ccttatattg taccaggaac cgttctaggg
     2701 attgccttca tttctctggt ctatttggaa gtggatttct tatgattaca gggactgctt
     2761 tcatcttgat tatgtctcta tctgtcagaa gattacctta tactattcgc tcatctgttg
     2821 ctagcttaca acaaatagca ccaagtattg aagaagctgc tgaaagctta ggaagtagtc
     2881 gtctcaatac ctttgctaag attacaactc caatgatgct atctggtatc atttctggag
     2941 ccatcttatc ttgggtcaca atgatttcaa aactctctac ttctatcctc ctctacaatg
     3001 tcaaaacaag aacaatgact gtagctattt atacagaggt tctcagagga aattatggtg
     3061 tagccgcagc cttgtcaact atcctgactg ttctaacagt aggttccttg ctcttgttta
     3121 tgaaaatctc taaaagcaat agcattacac tttagttttc cacatcaaaa acagccgaaa
     3181 ggagtaccct cggctgtttt ttcttatctt gatattctta tcaaatccct agctcatggc
     3241 aagcaagtct agactgatta aatagagcct tcttccatct tctcacgtcc tagctctcgg
     3301 taactacggt caccgacaac ttcaacccta aactgaccat cctcatattc aagaatcgtc
     3361 acgctaccat tatctagacc atgcggatgc atgccattaa tcagataaac aatggttcca
     3421 atagtcattc catggctgac aacaagggcg ttacctccac cttgatcttc catttctttt
     3481 gcaatcattt caaagccttc cttgattcgg ccactgagtt tttcccagcc ttcagcccaa
     3541 ccagctgtat cgacctctac caagccctca gccagttcag cataagacaa ttggtgaacg
     3601 tggtccacat taaagatacg aggaataatg cccatgaaaa gatcgccatc ataggctcca
     3661 tcaaaactac cgaaacacca ttctctgata cgcttgtcca tgcgataagg gattttcccc
     3721 tgcaagccaa gttcttcaag gataattccc atggtctgaa tggtacgacc agaatcactc
     3781 gaataagcgc gctcaaactg tagatcagat tctcgcaaac cgattcctaa ctcttgaatc
     3841 cctcgttcac cttcagcagt taagggagta tcgctccaac cttgcgcgcg accaatcgtg
     3901 ttaaacatgg tcttgccatg acgtaccaaa tacaatcgta cttttaccat tttccgtcct
     3961 ccgtttgctt ctattatatc atgaatgata aaataaaagc cacccataca ggcgactttt
     4021 gaaggagatt attatgaaaa agtttaggag ttcattaaat aaagatatta gatgaaaatc
     4081 aaattcaaac taattcagtg ttatctgttt caaataatat taggaggtct ttatttaatg
     4141 attatagtat acacatctaa tcttaaatag aacttaaaat ttctcctatt ttcttaattt
     4201 ttaaaactat gaattggtgc tgggatttgt ccaccgcgag agatgaagtc gacagacgaa
     4261 gccccattaa ccttcataac aggtgcagtt cctaatagac caccaaactc aatcatatcg
     4321 ccttctcttc ctttgggaat gatacgaaca gctgttgttt tcatgttgat aacaccgatt
     4381 gctgcttcat ccgcaatcat agccgcaatg gtttcagcag gcgtatcttc tgggatggca
     4441 atcatatcca atccaacaga acagatagcc gtcatagctt ctagtttttc taaattaaga
     4501 gagccatttt gcactgcagc aatcattcct tcatcctcag aaacagggat aaaggcgcca
     4561 gataaaccac cgacttggtt gcaggccatc actccaccct ttttaacttg gtcgttcaag
     4621 agggccaagg cagccgtcgt tccatgcgtg ccaactgttt ctagccccat ttcctcaagg
     4681 acacgtgcca cagagtctcc aaccgcaggg gttggtgcca aactcaagtc cacaatacca
     4741 aactccacac ccagtctctc actggccatt tgaccaacca attgaccgat acgagtgatt
     4801 ttaaaggcag ttttcttaac tgtttcggct actacatcaa agctctgtcc acgaactttt
     4861 tccaaagcac gtttcaccac accaggacca gaaactccga cattgatgat aacatctgct
     4921 tccccaacac catgaaaggc acccgccata aatggattgt cctcaacagc attagcgaat
     4981 acaaccaact tggccgctcc catatctgaa agatttgccg tttccttgat aattcgtccc
     5041 atatctgcca cagccgtcat attaatacca gacttggttg agccgatatt gactgacgag
     5101 cagaccttat ccgtctcagt caaagcgcga ggaatggaat tgatgagaat ctcatctccc
     5161 ttttgataac ctttttgtac taaggcagaa aaaccaccaa taaagtccac accaatctct
     5221 ttcgcagcct tatcaagcgc ttttgccaga accacgtagt ccgtcgcatc tgtcgctgcc
     5281 ccaatcagag aaataggtgt caccgataca cgcttattaa cgataggaat tcccaactca
     5341 gccgcaattt catcaccaac agctactaaa ttagccgcct ttgtcgtaat tttttgatag
     5401 attttctccg cagcacgatt gatatttgga tcgatacagt ccaaaagaga aatccccatg
     5461 gtaatggttc taatatcgaa gttttgctcc tcaatcatgg cgatggtttc agtaacttgt
     5521 ctaatatcca tgatgacctc ctagatatta tacatagctt cgaaaatcgc tgcactctga
     5581 atattgattt ttacattcaa agtttgccca aaagcttcaa attcattacg aagataggta
     5641 aaatcttgct tttcatcact agatacaaca gccatcatcg taaaatattc atccaataca
     5701 gtttgagaga tatcgtcaat attcaatcct aattctgcaa ttttaccaga aacacctgca
     5761 acaattccag atttatcttt accaacaaca gttataatgg ctttcataag taaactccaa
     5821 ttcttcttaa aataagaaac cttaacatta aacaggattc ttttcaaata gtatagcata
     5881 attctaacga aaatactcaa aaacgcctgc ttacgaagtt gttcttaaga aaaaaacaat
     5941 ttcgtaaaca agcgtctact atcccaactt atgatgagtg ttcccgctag gaccgaaatc
     6001 ctagcggtag accagagcta gactaagagc acaagactcc atcatcataa cactcaacaa
     6061 aattgatgat tttatactaa ttcgatgatc gccattggcg ctgcatcacc acgacgtggt
     6121 tcagttttaa ggatacgagt gtatccaccg ttacgttcag cataacgagg tgcgatttct
     6181 gagaacaatt tttgaagtgc tgtagtagaa gtgtacttat cagttgcttc atcatagttt
     6241 tcagatgcga tttcattacg tacgaaagca gctgcttgac gacgtgcatg caaatcacca
     6301 cgtttaccta gagtaatcat tttttcaaca gttttacgga tttctttagc acgagcttca
     6361 gttgtcacga ttgattcgtt gatcaaaagg tcagttgtca aatcgcgaag cattgcttta
     6421 cgttgtgagc tagtgcgtcc tagtttacgg taagccatgt attcctcctt tatttatctt
     6481 ttaatccaag acccaaatca atgagtttga gtttcacttc ttccaaactc ttgcgtccaa
     6541 gatttcgtac tttcatcatc tctgcttcag atttttctgt caaatcatgc acagtattga
     6601 taccggcacg ttttaaacag ttgtatgaac gcacagacaa gtccagttcc tcaatcgtac
     6661 gatctaaaat acggtcgtca gattcagtat cagcttcttt catcacttca gttgacttag
     6721 caatctcagt aagatttgta aacaaatcaa gatgttctgt caaaatacgt gctgaaagcc
     6781 ctaaagcatc ttctggaata attgttccat ttgtcaagat ttcaagggtt aatttgtcga
     6841 aaccatcatt gctacctaca cgagcaggtt ccacttgata gttgactttt gtaactggtg
     6901 tataaataga atctacagca agtgttccaa ctggtgcatt atccttttta ttttcgtcag
     6961 caggtacata tccacgacca ctgttaacag tcatagtcgc ttttagagaa gaaccttcac
     7021 cgattgtaaa gagataatga tctggattta caatttcaat atcgctatct gtcaaaatgt
     7081 caccagctgt tacttcagca ggaccttcaa catccagttc gatgattttt tcgtcttcaa
     7141 cgtacgattt cactgcaatt cccttaatgt tcagaatgat ttgcatcacg tcttcacgaa
     7201 cacctggaac tgtgtcaaac tcatgtaaca caccatcaat gttgatagat gtcacagctg
     7261 ctcctggtag agaagctaga agtacacgac gaagagagtt accaagagtt gtaccgtagc
     7321 cacgttcaag tggttcgatt acaaacttgc cataatcttt attttcatca atttttgtta
     7381 tatttggttt ttcaaactcg atcatttagt tactccctct taaacgaaaa gcagtgtaat
     7441 gcgatgatta tacacggcga cgttttggag gacgagcacc attgtgtggc actggagtca
     7501 catcacgaat tgctgttact tcaagaccag cggcagcaag cgcacgaata gctgactcac
     7561 gaccagaacc tggacctttt acagtaactt caactgattt aagaccgtgt tcttgtgcag
     7621 atttagcagc agcttcagaa gccatttgag cagcgaatgg tgtagattta cgagaacctt
     7681 tgaaaccaag agcaccagct gatgaccaag caattgcatt accatgcaca tcagtaatca
     7741 taacaatagt gttattaaat gtagcgtgaa tatgagcaat accagattcg atattctttt
     7801 tcacacgacg tttacgtgtt ggtttagcca agacttttac ctcctatatt attttttctt
     7861 accagcaatc gcaacagctt tacctttacg agtgcgagcg ttgtttttag tattttgtcc
     7921 acggacagga agtccacgac ggtgacggat accacggtat gaaccgattt ccatcaaacg
     7981 tttgatgttc aagtttactt cacgacgaag gtcaccttca actttgattg catccacttc
     8041 acgacggata gcatcttctt gatctgatgt aagatcacgt acacgaacat cttctgagat
     8101 tccagcagca gccaaaattt tcttagatgt tgcaagtccg ataccataaa cataagtcaa
     8161 tgagattact acgcgtttgt catttggaat atcaactcca gcaatacgag ccatgttttc
     8221 tcctttctat cttatccttg acgttgtttg tgttttggat ttgctgggca aattaccata
     8281 acacgaccat tacgacgaat aactttacag tattcgcaaa ttggtttgac cgatggtctt
     8341 actttcattt cttatccctc caagtttttc gattatttaa agcggtaagt gatacgtcca
     8401 cgtgtcaagt catatggact catttcgaca gtaacacgat ctcccgctaa aatacgaata
     8461 tagtttttac gaattttacc agaaactgtt gctaaaatct gatgtccatt ttcaagttca
     8521 accgtaaaca ttgcattcgg cattgtatca actactttgc cttcaacttc aatcacatcg
     8581 tcttttgcca cgcaaaagca cctccataaa tttcgattcg atgcctctag acacagagac
     8641 aacaattata agtcagacta tttcagtata acatttgtag cggatttttg caagtgtgaa
     8701 aaacgcttta tttcaaattt gtcaatactt tttcaatatc tgagaagaca tcattgatat
     8761 cttgattacc ttcgatgtca tgaaccaaac ctttggcacg gtagtgagca atgattggtt
     8821 ctccttgagc aatattaaca tccaaacgac gttttactgt ctcaggctta tcatcttcac
     8881 gttggtagta atcttcttct ttatagtcaa ctggtgggtt aaagaccttg tggaaagttt
     8941 ctccagttac gcggtggatg atacggccac tcaaacgttc caagaggctg tcagggttaa
     9001 cttcaatatt gataatacct tctagttcaa tgccaagttc agccaatgtt ttgtccaagg
     9061 catgagcttg ttcaattgta cgtgggtaac catccaataa gaatcctgtt tctttaatat
     9121 catcttgtga aaggcgttct tttacgattc catttgtaac ttcgtcagga accaattcac
     9181 ccttgtcaat atatgactta gcaagaacac ccatttcagt ttgatttgcc attgcagcgc
     9241 ggaacatatc acctgttgag atatgtgcaa catggaattg ttctacgatt tttgctgctt
     9301 gagttccctt acctgcacca ggtaagccca taatcaaaag attcataatt tgatctcctt
     9361 attttgtttt taaatagaag caaaaagttt tttcaccccg atttaaaaac aaaatagaag
     9421 aggggagatc caactcttac aaatgtcaga gtttaaacct ccactccctc agtatttact
     9481 gattcagtaa atacttttat tctgttctgt ccatgaaacc aacatactta cgtttcaata
     9541 ggtaaccttc caattgcttg attccttcga tacctgtaga gataatgatc aagagacttg
     9601 ttccaccaaa ggcaacaaca tcagaaagac caaatacatc tttagctgca atcggtaaaa
     9661 tggaaatcac accaaggaag agggaaccaa cagttgcaag acgacgaaga agtttagaca
     9721 tatattcttc tgtaccttta ccaggacgaa ctccatggat ataggcacca ctcttttgta
     9781 ggctctctgc tgctttttca ggattaatct gtacaaacgt atagaagaat gtaaagagaa
     9841 taatcaacaa agcatacatg gcaataccag ttggagaagt agttgccaac atctcttgtg
     9901 ctacccttac ccaagcccaa tcatgacctg tggcactcaa aaactgaaga atagccgcag
     9961 gcgctgcagt aatcgaactg gcaaagataa cagggataac tccagcagga tttaccttta
    10021 acggaaggta agagctagat ggagcacctt gtgcaacctt agtatattgg attggaattt
    10081 tgtattctgc ttgttgaaca taagttgtaa agtaaataat caacaataca gtaataatca
    10141 aaatgattac gaaaatgata gatgaagtga tacggctact tgggacgttc acaaagtagt
    10201 ccacatagat gccctgaatc atctctggaa ttgaggaaac aatcccggca aagataatca
    10261 tggaaacacc gtttccgtat cccttatctg taatttgctc acccaaccaa gtgacaatca
    10321 tactaccagc tgttaagatg ataccaatcg tcagaaaaac ttgtggagtt aaagcagttt
    10381 taatcaattg agctccagcc aaggtattaa aaccagctgt aatcccgata gattgcacaa
    10441 aagcgagaac tagagcaata taacgagtag cttgattcaa ttttcttcga cctacttccc
    10501 cttgtttacc ccactctaca aacttgggta aaatatccat ttgcaagagt tggacaacaa
    10561 tagaagcggt gatataggga ctaactccta gggcaaaaat cgaaaagttt tttagggcat
    10621 tccccgacac caagctcaac atgtttaaga aggataatcc acttaaagca ttcaagctat
    10681 tggcattcac accaggaact gtaatgctag ttccgatacg aaagaccaaa acgataaaaa
    10741 ttgtaaataa aatttttgat cgaacctgct tgactttaag agcttctctt aataatttaa
    10801 aaaacatagg tcacctctct tagatgactt ctactgaacc acctttagca gtgatagctt
    10861 cttcagctga tttagagaat ttagctgctt tcacagtcaa tttcttagtc aactcaccgt
    10921 taccaagaat tttaatacct gacttttcag ctttaacaat tcctgcttcg ataagaacaa
    10981 ctggagttac ttcagcacca tcttcaaaga cgttcaattg gtcaaggttc acaattgcgt
    11041 attctttagc gttgatgtta gtgaatccac gttttggaag acgacggaac aatggagttt
    11101 gtccaccttc aaaaccaagg cgaactccgc caccgctacg agctttttga cctttttgac
    11161 cacgaccaga tgttttaccg ttacctgatg aagtaccacg accaacgcgg ttacgtactt
    11221 tacgagaacc ttctgcaggt ttcaattcat gaagtttcat ttttattttc tcctcttttg
    11281 taaaatgcta gcgccgataa gggagaaaag gttgtctccc ccatcaactc gcctatacga
    11341 tgtcatcata gatgactata tctagtttta ggggatggta cagtgcacat cccctaaaac
    11401 ttcattagtt tacttcttca actgttacta agtgagatac tgctgtgatc ataccacgga
    11461 tagcagcgtt atcttcttta ataacagagc tgttcaattt gccaagtcca agtgctacaa
    11521 cagttttacg ttgtgatgga atgcgtccga ttggagactt agtcaaagta attttaattt
    11581 gagccatttt atcccctttc ttatgccaaa tcagaaactg aaataccacg aagggcagca
    11641 atttcttcag cgcgtttcaa ttgtttcaaa ccttcaacag ttgcacgaac aatgttgatt
    11701 ggagtgttag aaccaagtga tttagatgta atatctgcca cacctgccaa ttccacaacg
    11761 gcacgaactg caccaccagc ggcaactcca gaaccttcta cagcaggttt caacaatact
    11821 ttagctccac cgaattctga aagaacttcg tgtgggattg ttgttccaac cataggaact
    11881 tcgatcaagt ttttcttagc atcatctact gctttacgga ttgcttctgg aacttcttga
    11941 gctttaccag taccaaatcc tacgcgacca ttgtggtcac caacaacaac aagagctgcg
    12001 aaacgaagac gacgtccacc tttaacaact tttgtaacac ggttgacagc aactacgcgt
    12061 tcttctaatt caactgcatt gtctttaaat gccattttct agtgtcctcc tattagaatt
    12121 tcaatccgtt ttcacgagct gcatcagcca aagctttcac acgtccgtga tatagatatc
    12181 caccgcggtc gaacaccact tctgaaatac ctttagcgtt tgcacgttct gcaacgagtt
    12241 taccgacagc aacggcttgt tcagttttag ttccttttga aacttcttta tcaagagttg
    12301 aagcacttgc gagcgttaca cccgctacgt catcaatcac ttgagcgtag atgcctgtat
    12361 tagaacggaa tacgttcaaa cgtgggcgat cagcagttcc agagagtttt ccgcgaacgc
    12421 gacggtggcg tttttggcgg agtttgtttt tatctggttt tgaaatcaca gttttcacct
    12481 ctttagtttt aaatcgtgtg ctatgcacaa agttggaaaa taggttggtg gttgatgatc
    12541 aaccactcaa cattatttac ctgttttacc ttctttacgg cgaacgaatt caccaacgta
    12601 acggatacct ttacctttat atggttctgg tgaacgaagg ctacgtacgt aagcagctgt
    12661 ttgaccaact acttcttttg aaattccgct aacaacgatt gttgttgggt ttggaagttc
    12721 aaaagtaatt ccttctggag cttcaacttc gtctggatga gatttaccaa cagccaaaac
    12781 aagtttagat ccttgaagct gtgcacggta accaacccca cgcatttcaa gttctttctt
    12841 gaatccttct gatacaccaa caaccatgtt gttcaaaagg gcacgagtag ttccgtggat
    12901 agttttcatt tcttttgaat cgtttggacg gtgaagagtt acttcagtac cttccacacg
    12961 gatttcaata tcttttgaga actcacgagt aagttctcct ttaggtcctt ttacagttac
    13021 aacgttgtca ttgttagcga gttcaacacc agcaggcaac acgataactt tattaccaat
    13081 acgtgacatg tttattttct cctgttaaat tgtcaggcca aaacggccag ttttcacggg
    13141 gttcagatac ttatttagtt caagagctga actgaacacg ctctaagaac tgtgcaaaat
    13201 agataagttg gcttgtttgt aaacaaactt ctccaacttc ctaattttgc tttgttcttt
    13261 acgagctttg tatcttgatt ttaccaaacg taagcgataa cctcaccacc aacattcttt
    13321 tggcgtgctt ctttatcagt aagcaaacct tcagaagttg aaaggatggc aattccaagt
    13381 ccgttaagaa cttttggaag gtcttcacgt tttttgtaga cacgaagtcc tggtttagaa
    13441 acacgtttca agttagtgat aactttctca ccatttggtc cgtatttaag aaatacacgg
    13501 atgacgcctt gtttgtcatc ttcaatgatt tcaacgtttt ttacaaaacc ttcgcgttta
    13561 aggatttcag caatcccttt tttgatgttt gatgcaggta cttcaagtac ttcgtgttta
    13621 gcttggttag cattacgaat acgagttagg aagtctgcga ttgggtcagt cataaccatt
    13681 ttgtttttct cctcttacta gtagtttgca agttgcactt gctagttaat acatgataca
    13741 aagagcgtaa cagcacgagc aaaaatagac aatttaatgc agaagcaatg ctccaaagca
    13801 aattggtctt ttttgccaaa gctgtagctc gtgttcaaat tggcaagccc aattgaaccc
    13861 gggctaaact ctgtgtgaaa aagataaact ttcctagaaa ctttagtttc ttcgtcaagt
    13921 ttcctatttt tacttggagt tttgacgctc ttgatatctt aaattaccaa gatgcttttg
    13981 ttacaccagg aatttgacct ttatgcgcaa gttcgcgaaa agcgatacga ctcagaccaa
    14041 atttgcggta aactgaatgt gggcgccccg taactctaca acgattatgt aaacgagtcg
    14101 gtgaggcgtt gcgaggtaat ttagataaac cttcgtagtc ccctgccgcc tttaatgcag
    14161 cacgtttttc agcataacgg tcaacaattt tttggcgttt agcctctcta gctaccattg
    14221 attttttagc cattagattt acctcctata ttattttgca aaaggcattc caaggcctgt
    14281 aagcaatgca cgtgactctt cgtcagtgtt agcagttgtt acgataacga tgtcaagacc
    14341 acgagttttg tcaacgtcat cgaagttaat ttctgggaag attaattgtt ctttcacacc
    14401 aagtgtgtag ttcccgcgtc catcaaatga ttttgttgga acaccgtgga agtcacgtac
    14461 acgcggaagt gaaactgata ccaatttatc caagaattcg tacatacgtt caccacgaag
    14521 ggtaactttt gcaccgatcg caacaccttc acgaagacgg aagccggcga ttgatttttt
    14581 agctttagtg ataagtggtt tttgacctga gataagtgcc aattcttcag cagctttttc
    14641 aaggctttta gcgtttgata cagcttcacc aacacccatg ttcaaaacaa tcttatctac
    14701 tttaggcaca gccatcactg atgagtagtt gaattgttct gtcaaagcag gaactacttc
    14761 attaagatat ttttctttta aacgatttgc cattatactt ctcctttcct tcgtgattaa
    14821 tcaagcactt cgcctgattt tttgttgtag cgaacttttt taccgtctac aaatttgtat
    14881 ccaacacgac cagctacacc atttttgtcc aaaacttgaa cgtttgatac gtggatagct
    14941 gcttctttct cgatgatacc accttgagga agctcgttag ttggacgttg gtgtttctta
    15001 acaatgttaa caccttcaac gataactttg tttacttttg gaagggcagt aaggacaaca
    15061 gcttctgttc ccttatcttt accagcgatt acgcgaactt tgtcgccttt ttttacaaac
    15121 attaggtttc tccttgattt ttcttacgcc cataagggca ccctagcttg aagctagggg
    15181 actagtttgt tcctaaaaat taaagtactt ctggagcaag tgacacgatc ttcatgaagc
    15241 caccttcacg caattcacgt gcaactgggc caaagatacg tgttccgcga ggagttttgt
    15301 cttcacggat gataactgct gcgttttcgt caaatttgat gtatgaacca tcagcacgac
    15361 gagcacctga tttagtacga acgataactg ctttaacaac gtcacctttt ttaaccgcac
    15421 caccaggagt agcttgtttt acagatgcca cgataacatc accgatgttt gcaaatttac
    15481 gtcctgaacc accaagaact ttgatagtca agatttcgcg agcaccgctg ttgtctgcga
    15541 ctttcaaacg agtttctgtt tgaatcattt cagttttctc ctttcaggtt tgattagatg
    15601 atgaccgctt cttcaacaac ttctacaaga cggaaacgtt ttgtagctga aagcgggcga
    15661 gtttccatga tacgtacgat atcgccttct ttggcaacat tgttttcatc atgagctttg
    15721 tattttttag agtagttaat acgtttacca tagactgggt ggttacgttt tgtttcaact
    15781 acaactgtga ttgtcttgtc cattttgtca gatacaacac gtccaacaag aactttacga
    15841 ttattgcgtt ccattgaaat ttctccttcc ctagtctatt atttcgcttc agattgaact
    15901 gttttgatac gagcgatttg ttttttaact tctttcaagc gagctgtttg ttccaattga
    15961 ccagtagcag cttggaaacg aagttcaaac aattcttttt tcaattcgtt ttcgcgcttc
    16021 gcgagttctt cttgagaaag accacgaagt tctttaacaa attcttttac ttcattaagt
    16081 ttcatgcctt ctccttattc tgcttcacgt tttacgaatt tacatttaac tggcaatttg
    16141 tggctagcaa gtcgaagcgc ttcacgtgca atctcttcag atacaccagc gatttcgaac
    16201 atcactttac cacgtttaac tggtgctacc caaccttcag gtgccccttt accagatccc
    16261 atacgcacac cgatagcttt agcagtgtat gatttgtgtg ggaagatttt aatccaaact
    16321 ttaccaccac gtttcatgta acgagtcatg gcgatacgag cagcttcgat ttggcggtta
    16381 gtgatccagt ggctagttgt agcttgaaga ccgtattcac cgaatgctac ttcttttcca
    16441 ccttttgctt caccgcgcat ttttccacgg aactcacgac ggtgtttaac acgtttaggt
    16501 actaacattg gttatttacc tcctttagtg tttttacgag ctggaagaac ttcaccacgg
    16561 tagatccata ctttaacacc aagtttaccg tatgtagtat ctgcttcttc ccaagcgtaa
    16621 tcgatatctg cacgaagtgt gtgaagcgga acagttcctt cagagtatcc ttcagcacgg
    16681 gcgatatctg caccgttcaa acgacctgat acttgagttt tgattccttt agctccagca
    16741 cgcattgcac gttggattgc ttgtttttgt gcacgacgga aagcaacacg ttgctccaat
    16801 tgacgagcaa ttccttcacc tacaaggtga gcatccaaat caggttgttt gatttcgatg
    16861 atgttgatgt gtacttgttt tccagtcaat ttgttaagtt ttgcacggag tgcatcaacg
    16921 ttagcaccac ctttaccgat aaccatacct ggtttagcag tgtgaagtga aacgttaact
    16981 ttgtttactg cgcgttcgat ttcaatagtt gaaactgctg cgtcagcaag ttctttttga
    17041 acgaatttac ggattgcaag atcttcatga aggtaatccg cgtattcttt ttcagcatac
    17101 catttggcat cccaatcacg gatgatgccg acacgcatac caattggatg tactttttga
    17161 cccacgattt tacctcctta tttttctgca acagctacag tgatgtgagc tgtacgtttg
    17221 ttgattggtg aagctgaacc tttcgcacgt ggacggaaac gtttcatagt tggtccttcg
    17281 tttgcgaatg cttcagatac taccaagtta gctttatcca aaccaaagtt gttttcagcg
    17341 ttagctacag ctgagttcaa aactttcaag atgatttcag cagctttgtt tggagtgaat
    17401 gtcaagattg cgattgcatc ggctacgctt ttaccacgga tgttatcaag aacaagacgt
    17461 gatttacgag gtgaaacacg tactgtacga gccattgctt tagctgaagt aatttctgcc
    17521 atttatgttc tccttatttt ctacgtgttt tcttgtcgtc tgcagcgtga cctttgtaag
    17581 tacgagttgg tgcaaattca ccaagtttgt ggcctaccat gtcttcttgg atgtaaacag
    17641 gtacgtgttt acgtccgtca taaactgcaa tagtgtaacc aatgaaactt gggaagatcg
    17701 ttgaacgacg tgaccaagtt ttaataactt ttttcttttc gtcgttagct tgagcttcaa
    17761 cttttttcat caaatgctca tcgacgaaag gtcctttttt aagactgcgt cccattttta
    17821 tattttctcc tttaaatgtt gtaccacagc ggcttgcact cctatggagc gctaccgagc
    17881 tggcggattt actagttgct taagcgacta gtttaatatt atttctcgtt gcgacgacga
    17941 acgataagtt tgtcagattt cgctttcttg ttacgagttt taagaccaag agcaggtttg
    18001 ccccatggag tagatggtgc tttacgacca actggtgctt taccttcacc accaccgtgt
    18061 gggtgatcgt tagggttcat tacagaacca cgaactgttg ggcggatacc tttccaacgg
    18121 ctacgtcctg ctttaccaag gtttacaagt ccatgttgtt cgtttccgac aacaccaact
    18181 gtagcacggc aagttccaag aatcatacga acttcacctg attgaagacg aacaagaaca
    18241 tatttacctt cagaacccaa tacttgagca gatgcaccag cagcacgtac caattcacca
    18301 ccacgacctg gtttcaactc gatgttgtga atcaaagtac caactgggat gttagcaagt
    18361 ggaagagcgt ttccgacttt gatatctgct tctggacctg aaacgatacg ttgacctact
    18421 tcaagacctt ttggagcgat gatgtatgct ttcacaccgt cagtgtagtg tacaagagcg
    18481 atgtttgcag aacggtttgg atcgtactcg attgttttaa caactgcttc aacgttgtct
    18541 ttattacgtt tgaagtcaac caaacggtag aaacgtttgt gtccaccacc ttggtgacga
    18601 actgtgatac gaccgttgtt gttacgacca gccttgctct tcaatgcaac aagcaatgat
    18661 ttttcaggag tgcttgttgt gatttcagcg aaatccaaag aagtcatatt acggcgaccg
    18721 tttgttgttg gtttataaac acgaattccc acgatatttc ctccttagat tattcagctt
    18781 cagcagcaaa caactcgatt gctttagaat cagctgtaag tgtgatgata gcttttttag
    18841 ttttgttagt aaaaccagtg taacgtccaa cacgtttagc ttttggtttt acgttgattg
    18901 tgttaacatt ggcaacttta acaccttcga aagcagcttc aacagcttgc ttgatcaaaa
    18961 gtttgtgtgc acgagtgtca acttcaaata catatttccc tgcttcaagt tgagccattg
    19021 agctttcagt gatgacaggt tttttgataa catcatacaa attcattatg caagaacctc
    19081 ctcgatttta gagatagctg cttgtgtgac aagaagtttg tcgctatttg cgatgtcaag
    19141 aacacttgca gttgtagcag ttgcaacttt cacgtttgga aggttacgag ctgaaagagc
    19201 tgcgaattca tttccttctt caaggataac aagaacttta gaatcgatgc tcaatgctgc
    19261 aagaactttt gcaaattcag cagtttttgg agctgtaaat gaaagagcgt ctacagctac
    19321 gaatttgttt tcagcaactt tttcagagta aactgattta agagctaggc gacgaacttt
    19381 ttgtggaagt ttgtagccgt atgaacgtgg agttggtccg aagacaacac caccaccacg
    19441 ccattgtggt gagcggatag aaccttgacg agcacgtcca gttccttttt gacgccatgg
    19501 tttgcgtcca ccacctgata ctgcagagcg gtttttaaca gcgtgtgttc cttgacgaag
    19561 gcttgcgcgt tggctgatga ttacatcaaa cacaactgat tcatttggtt cgataccaaa
    19621 tactgcatcg ctaagaacaa cttggccagc ttctttacca gtttggtcaa ataatgttac
    19681 gtttgccatt gtgactgatt tcccctttct ttattattta ccagctttaa ctgctgattt
    19741 gatagtgata agagatttct tagcacctgg tacgttacct ttgataagga taacgttctt
    19801 ttctggaaca acttgtacaa cttcaaggtt ttgaattgtt acgcggtcgc cacccatacg
    19861 ccctgcaagg tttttacctt tgaatacgcg gttaggtgca acaggtccca tagaacctgg
    19921 acgacggtgg taacgagaac cgtgagccat tggtccacgt gattgtccgt ggcgtttgat
    19981 aacaccttgg aaacctttac ctttagaagt accagttacg tcaacaacgt ctccagctgc
    20041 gaatgtttca actgtaattt cagcaccaac ttccaagcct tcaacgtttt tgaattcacg
    20101 aatgaagcgc ttaggagccg tgttagcttt cgctacatgt cctttagcag gtttgttgct
    20161 caatacttcg cgtttgtcat cgaaaccaac ttggatagcg ttgtatccgt ctgtttcaac
    20221 agttttaact tgaagaacaa cgtttggagt tgcttcaata actgttacag ggatcaattc
    20281 gccagcttca gtgaagattt gagtcattcc cacttttttc cctaagattc cttttgtcat
    20341 gagaaaatag ttccttttct atatttttta ttcaaaaagt ttttaacgag cgttttttat
    20401 gctcaagtct aagatacaaa gggcgtcaaa ctcaaagtga aaataggaaa ctgacgcagt
    20461 gtctagcaga cactaggaag tttatctttt tcacaccgag tttagtccgt gttcaattag
    20521 attgaaacac cagcctctgc tcttttatat tttagattaa agtttgattt ctacgtttac
    20581 accacttgga agatccaatt tcatcaaggc atcaactgtt ttttgagttg ggttaacgat
    20641 atcgatcaaa cgtttgtgtg tacgcatttc aaattgttcg cgagagtctt tgtatttgtg
    20701 agtcgcacga atgattgtgt agaggctacg ttcagttgga agtgggattg gacccgcaac
    20761 ttgtgcacct gtacgagtag ctgattctac gatttttgca gccgctgtgt caagcgtacg
    20821 gtgttcgtaa gctttcaaac ggatacggat ttttttgttt gccatctttt tctccttttc
    20881 gtctatttaa gataataggc tagctccaca agaaaaccga cgcggtgttg cgtggcaatg
    20941 caaccgagcg tgtcgcaacc tcttgcatca aaactaaggc tgtaatttac agcaccataa
    21001 tagaataaca caaatcccct gcgattgcaa gggatttgtt aggttttttc gtttttttgg
    21061 gatgaaactg ttttcctact ttttctttct aaaattaggg attttcttct atatttcttc
    21121 tatacataag ttaagtcctc ttgacatcaa atttatcttc tgattggtcc actaagatat
    21181 ccgccttaga ctcagtttct ttatagtatc gcagatactg ctcccgtctc atctgatggc
    21241 tagccaatat aaaggatgca tcgcgatttc tcacagtcgt atctctagca aggcgtcgct
    21301 ttaattcggt ctcctcatcc gtgtagaaac agatggtttt ttcaaagagt tccttgggta
    21361 gaaagccaac agacatccct tcgacaatca aaattggttt ggctccagac aagacctcac
    21421 tagccttcca aggttcttca attgtcaaga catccatacc cgcctgcaag gcaaggatat
    21481 ctctctgcaa actctccagt tcatgcgcca ctggcagact ggctgtcacc ttttgattcg
    21541 gcgcgtcctt gggtactacc agatggcgtc ctgaggtgat ataaggatct gtctctagca
    21601 aatttactgt agtagaatct aaagcttggt acaattcctg tgcaaaggtt gatttacctg
    21661 aagctccatg accgtatatt cccagtgtcc tgactttccc cgtctcgatc tctgagacta
    21721 gttggtctac taagtctttt ttcttcattc tctcttcacc tgttcatagc tttcttttcc
    21781 gtcattgagc ttgtcccaaa tcactacttg cccacttttg agcgattttt gcacatcgat
    21841 aattcgttgg ttggacgaac ctcgaaactg gagcatgaga tttctcttag ttcgatcgta
    21901 tcgtccatcg acaagaatgt caatcaatga taagagttcc agtttatctg gagtttccaa
    21961 catcatttct tcccaagtgt agccggtcca ggaccaaatg tccttgtctg gcaattcctt
    22021 tcggatgcgt ttaactagag gcaagagaat gccagtatta agaaaaggct cccctcccag
    22081 caaagtcaag ccttgaacat agggttgggc aaggtctgcc ataatctgct cttctaattc
    22141 tgctgtatag agaatgccag cattaaaaga ccaagtcgca acattataac atccctcgca
    22201 gtgaaacata cagcctgata catagagaga gttacgcacg ccttcgccat caacaaagtt
    22261 aaaggccttg tagtcaatga tacgaccttg actaagttcc tcgcttttcc attcttgtgg
    22321 ttttggatta ttcattcgct acctctatcc aataacgctc gactccattg tgagcatcct
    22381 caaatattcc accatttgct agaatgactg ctctgctagc aggattattc acgctacagg
    22441 tcaccagagc tttcttgatg ttcttttcct tagcaacttg caagccctga cggagagtct
    22501 cttttgcata acccttgcct ctttcagatg gacggatgga gtagccaatg tgcccacctt
    22561 cttctagtag aaagttactg aggcgcaacc ggagattaag aaatccaact gcttgacctt
    22621 tctcagaaaa agccactaac tgaattgcag gaacccatcc ttcaggcaga ttaatcccca
    22681 tttcctgttc ctgattgctt tccaaccact cttcatacac aaaattctct gtatcccaaa
    22741 atcctccatc atgggctgat tggctctttt caaactctgt catcatatct aaaacagctt
    22801 tcttatccgc taatcttggt ctgcgtaatt ccatccttac ctcccactat gagaaaagcg
    22861 agagctggct ctcactttta ttttttcttg ttcttgttta aaaattgttc tctgaagcta
    22921 agcaagcgtt cttttttacc agctccacct ttagagtgtt tctcgtgata acgggtcact
    22981 tgtgcgcgcc ccttatcgtc t
//
LOCUS       DAVAAL010000019        22695 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVAAL010000019 DAVAAL010000000
VERSION     DAVAAL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22695
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.019"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            76..732
                     /locus_tag="VQM60_000616"
     CDS             76..732
                     /locus_tag="VQM60_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET1080506.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            722..1045
                     /locus_tag="VQM60_000617"
     CDS             722..1045
                     /locus_tag="VQM60_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET1080507.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1149..1979
                     /locus_tag="VQM60_000618"
     CDS             1149..1979
                     /locus_tag="VQM60_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET1080508.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2133..2987
                     /locus_tag="VQM60_000619"
     CDS             2133..2987
                     /locus_tag="VQM60_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET1080509.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3087..4460
                     /locus_tag="VQM60_000620"
     CDS             3087..4460
                     /locus_tag="VQM60_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET1080510.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFDPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4453..5514
                     /locus_tag="VQM60_000621"
     CDS             4453..5514
                     /locus_tag="VQM60_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET1080511.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5516..6208
                     /locus_tag="VQM60_000622"
     CDS             5516..6208
                     /locus_tag="VQM60_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1080512.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6238..6807)
                     /locus_tag="VQM60_000623"
     CDS             complement(6238..6807)
                     /locus_tag="VQM60_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET1080513.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            6942..7556
                     /locus_tag="VQM60_000624"
     CDS             6942..7556
                     /locus_tag="VQM60_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET1080514.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7558..9204
                     /locus_tag="VQM60_000625"
     CDS             7558..9204
                     /locus_tag="VQM60_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET1080515.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLNPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9216..10502
                     /locus_tag="VQM60_000626"
     CDS             9216..10502
                     /locus_tag="VQM60_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1080516.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATVSHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAEFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKDLFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10563..11096
                     /locus_tag="VQM60_000627"
     CDS             10563..11096
                     /locus_tag="VQM60_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1080517.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRTAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11174..11644
                     /gene="nrdI"
                     /locus_tag="VQM60_000628"
     CDS             11174..11644
                     /gene="nrdI"
                     /locus_tag="VQM60_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET1080518.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(11937..13199)
                     /locus_tag="VQM60_000629"
     CDS             complement(11937..13199)
                     /locus_tag="VQM60_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET1080519.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(13509..13967)
                     /locus_tag="VQM60_000630"
     CDS             complement(13509..13967)
                     /locus_tag="VQM60_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET1080520.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(13964..14404)
                     /locus_tag="VQM60_000631"
     CDS             complement(13964..14404)
                     /locus_tag="VQM60_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1080521.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            14760..16709
                     /gene="mutL"
                     /locus_tag="VQM60_000632"
     CDS             14760..16709
                     /gene="mutL"
                     /locus_tag="VQM60_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET1080522.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(17034..17501)
                     /locus_tag="VQM60_000633"
     CDS             complement(17034..17501)
                     /locus_tag="VQM60_000633"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET1080523.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(17502..18707)
                     /locus_tag="VQM60_000634"
     CDS             complement(17502..18707)
                     /locus_tag="VQM60_000634"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET1080524.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRIDDLIQFSKKHNIPLITIKELQEYRKAYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKIIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(18727..19362)
                     /gene="ribE"
                     /locus_tag="VQM60_000635"
     CDS             complement(18727..19362)
                     /gene="ribE"
                     /locus_tag="VQM60_000635"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET1080525.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(19347..20447)
                     /gene="ribD"
                     /locus_tag="VQM60_000636"
     CDS             complement(19347..20447)
                     /gene="ribD"
                     /locus_tag="VQM60_000636"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET1080526.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGSHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMTIDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(20581..20695)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            20852..21445
                     /gene="ruvA"
                     /locus_tag="VQM60_000637"
     CDS             20852..21445
                     /gene="ruvA"
                     /locus_tag="VQM60_000637"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET1080527.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKIAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            21455..22018
                     /locus_tag="VQM60_000638"
     CDS             21455..22018
                     /locus_tag="VQM60_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET1080528.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            22015..22692
                     /locus_tag="VQM60_000639"
     CDS             22015..22692
                     /locus_tag="VQM60_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET1080529.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
BASE COUNT         6557 a         4329 c         4919 g         6890 t
ORIGIN      
        1 ttcattgagt attattaaga caatatgagg agaaagatga aagaaaaagg attttaggag
       61 ggggcgcagg cagccatgcc aacggctctt ggctatgtca gtatcggcct ggcctgtggg
      121 actatcggtg cgccctatgt gacacctgtt gagatgggct tgatgagtct ctttgtttat
      181 gctgggagtg cccagtttgc catgttggca ctgattgtgg ttcaagctcc tgtggcagct
      241 attgctatga cggtttttct aatcaacttg cgtctctttt tgttgagttt acacgcatcg
      301 acttatttcc gtcataccag tctttggtac aatatcggta tgtctagtat cttgacagat
      361 gagacctatg gcgttttgat gggtgaattg gcccatacag acaaggtaaa tcctatgtgg
      421 atgcacggaa acaatcttaa cagctatgtg gcttggtttg tggggacagt agtcggaacg
      481 gctctgggtg gcctgctacc aaatccagaa atctttggct tggattttgc cctggttggg
      541 atgtttattg gtatttttgc ttcgcaattt cagattatgc aaagacggat tcctgtccgc
      601 aatctgctca ttatcctagc agttgttgcg gtgtccttct ttttgctctt gacagtggtg
      661 tctcagtcac tagctgttct gtttgcgacg ctacttggtt gtagcatggg ggtggtttta
      721 gatggtcagt aagtatcttt tattagcagt tattttctct ggcttggtga cttggattct
      781 ccgtatgatt cccttcatct tagtcaagta taagggcttg cctgcaatcg ttgagcgttt
      841 tttgaagttc ttgcccgttt ccattatctt tgccttgatt ctttcaagcg tagtgacagg
      901 taaggttggg agccttcctc aaattaaatg gctagacttc ttagccgtct ttccaacagc
      961 ttgggtagcc tttcgctacc gcaatctagt cggaacagtt ctctttggag tggtcttgat
     1021 tgccatcttg cgtttggtct tttaaattac ccataaaaaa aacttatcac agagatagat
     1081 atcatataat ggcgtaaatg ctctttttct gttaagatta taaggtattc tattttggag
     1141 gaaatgacat gaaaaaaatc gttaaatact catctcttgc tgccctagga cttgttgctg
     1201 caggtgtgct tgcggcttgc tcagggggtg ctaagaaaga aggagaagca gctagcaaga
     1261 aagaaatcat cgttgcaacc aatggatcac caaggccatt tatctatgaa gaaaatggcg
     1321 aattgactgg ttacgagatt gaagtcgttc gcgctatctt taaagattct gacaaatatg
     1381 atgtcaagtt tgaaaagaca gaatggtcag gtgtctttgc tggtcttgac gctgaccgtt
     1441 acaatatggc tgtcaacaat cttagctaca ctaaagaacg tgcggagaaa tacctctatg
     1501 ccgcaccaat tgcccaaaat cctaatgtcc ttgtcgtgaa gaaagatgac tctagtatca
     1561 agtctctcga tgatatcggt ggaaaatcga cggaagtcgt tcaagccact acatcagcta
     1621 agcagttaga agcatacaat gctgaacaca cggacaaccc aactatcctt aactatacta
     1681 aggcagactt ccaacaaatc atggtacgtt tgagcgatgg acaatttgac tacaagattt
     1741 ttgataaaat cggtgttgaa acagtgatca agaaccaagg tttggacaac ttgaaagtta
     1801 tcgaacttcc aagcgaccaa caaccgtacg tttacccact tcttgctcaa ggtcaagatg
     1861 agttgaaatc atttgtagac aaacgcatca aagaacttta taaagatgga actcttgaaa
     1921 aattgtctaa acaattcttc ggagatactt atctaccagc agaggctgat attaaataat
     1981 ttcttgaaat catccattct gagtaagatg ggtgatttct cagtttttag ggatatagtt
     2041 ttaaactata tatctgatta tctaataaca atttgtgaaa tcaaacaaat tgtgagatac
     2101 tagtacggta ttatttttaa ggagaaagaa tcatgaaaat caaaaaatgg cttggcctag
     2161 tagcccttgc tacagtcgca ggtttggctc ttgcagcttg cggaaactca gaaaagaaag
     2221 cagacaatgc aacaactatc aaaatcgcaa ctgttaaccg tagcggttct gaagaaaaac
     2281 gttgggacaa aatccaagaa ttggttaaaa aagacggaat taccttggaa tttacagagt
     2341 tcacagacta ctcacaacca aacaaagcaa ctgctgatgg cgaagtagat ttgaacgctt
     2401 tccaacacta taacttcttg aacaactgga acaaagaaaa cggaaaagac cttgtagcga
     2461 ttgcagatac ttacatctct ccaatccgcc tttactcagg tttgaatgga agtgccaaca
     2521 agtacactaa agtagaagac atcccagcaa acggagaaat cgctgtaccg aatgacgcta
     2581 caaacgaaag ccgtgcgctt tacttgcttc aatcagctgg cttgattaaa ttggatgttt
     2641 ctggaactgc tcttgcaaca gttgccaaca tcaaagaaaa tccaaagaac ttgaaaatca
     2701 ctgaattgga cgctagccaa acagctcgtt cattgtcatc agttgacgct gccgttgtaa
     2761 acaatacctt cgttacagaa gcaaaattgg actacaagaa agcacttttc aaagaacaag
     2821 ctgatgaaaa ctcaaaacaa tggtacaaca tcattgttgc gaaaaaagat tgggaaacat
     2881 cacctaaggc tgatgctatc aagaaagtaa tcgcagctta ccacacagat gacgtgaaaa
     2941 aagttatcga agaatcatca gacggtttgg atcaaccagt ttggtaataa gaaacaggga
     3001 ggtgggagag agaattccac ctcttgcttt tgtatagagc acggagtgta aagaagacta
     3061 ttcgttcata gaaaggtaga gagaatatgg tttttcctag cgaacaagaa cagattgaaa
     3121 aatttgaaaa ggatcatgta gcccagcatt attttgaggt tttgcgtacc ttgatttcta
     3181 agaaatcagt ctttgcccag caggttggac tcaaggaagt cgcaaattat ctgggtgaga
     3241 ttttcaagcg tgttggagct gaagtggaga ttgatgagag ctatacagcg ccctttgtca
     3301 tggcacattt caagagttcg cgtccagatg ccaagacctt gattttctat aaccactatg
     3361 acactgtgcc agcggatggg gatcaggtct ggacagagga tccttttacg ctttcggtcc
     3421 gcaatggctt catgtatggg cgtggagttg atgacgacaa gggtcatatc acagctcgct
     3481 tgagtgcttt gagaaaatat atgcagcacc atgatgattt acctgtcaat atcagcttta
     3541 tcatggaggg agcggaggaa tcggcttcaa cagacctaga taagtatttg gaaaagcatg
     3601 cagacaaact ccgtggggcg gatttgttgg tctgggaaca agggaccaaa aatgccttgg
     3661 aacagctgga aatttctggt ggcaataagg ggattgtgac ctttgatgcc aaggtaaaaa
     3721 gcgctgatgt ggatatccac tcgagttatg gtggtgttgt ggaatcagct ccttggtatc
     3781 tcctccaagc cttacagtct cttcgtgctg cggatggccg tatcttggtt gaaggcttgt
     3841 acgaagaagt acaagagccc aatgaacgag aaatggcctt gctagaaact tatggtcaac
     3901 gaaacccaga ggaagttagt cggatttatg gattggagtt gcctctctta caggaggagc
     3961 ggatggcctt tctaaaacgt ttctttttcg atccagcgct taatatcgaa ggaatccagt
     4021 ctggttatca aggtcagggt gttaagacta ttttacctgc agaagccagt gccaagctag
     4081 aggttcgtct ggttccgggc ctagaaccgc atgatgttct ggaaaaaatt cggaaacagc
     4141 tagacaaaaa tggctttgat aaggtagaat tatactatac cttgggagag atgagctatc
     4201 gaagcgatat gagcgcacca gccattctca atgtgatcga gttggccaag aaattctatc
     4261 cacagggcgt ttcagtcttg ccgacgacag cggggacagg acctatgcat acggtctttg
     4321 atgccctaga ggtaccaatg gttgcattcg gtctaggaaa tgccaatagc cgagaccacg
     4381 gtggagatga aaatgtgcga atcgctgatt attacaccca tatcgaatta gtagaggagc
     4441 tgattagaag ctatgagtag agatattatc aagttagatc agatcgatgt gacttttcac
     4501 caaaagaaga gaaccatcac agcggttaag gatgtgacca ttcacatcca agaaggggat
     4561 atctacggaa tcgttggata ttctggagca ggaaaatcaa cccttgtacg ggtgattaat
     4621 cttttgcaaa aaccatctgc agggaaaatt accattgacg acgatgtgat ttttgacggc
     4681 aaggtgacct tgacggcaga gcagttgcgt cgtaaacgtc aagatatcgg gatgattttc
     4741 cagcatttta acctgatgag ccaaaagaca gcagaggaga atgtagcctt tgcccttaaa
     4801 cactctggac tcagcaagga agaaaagaag gctaaagtag ctaagttgtt ggacttggtt
     4861 ggtttggcag atcgtgctga aaactaccct tcacaactat ctggagggca aaaacagcgt
     4921 gtggcaattg cgcgtgcctt ggccaatgat ccaaaaatct tgatttcaga cgagtcaact
     4981 tctgcccttg accctaagac aaccaagcag attttggcct tgttgcaaga tttgaaccaa
     5041 aaattaggat tgacagttgt cttgattacg catgaaatgc agattgtcaa agacattgcc
     5101 aaccgtgtgg cggttatgca ggatgggcat ttgattgaag agggaagtgt ccttgaaatc
     5161 ttctcaaacc ctaaacaacc tttgactcaa gactttatct caacagccac aggtattgac
     5221 gaagccatgg tcaaaatcga gaagcaagaa atcgtggaac acttgtctga aaacagtctc
     5281 ttggtgcaac tcaagtacgc tggagcttca acagacgagc cacttttgaa tgaattgtac
     5341 aagcattacc aagtaatggc taatattctc tatgggaata tcgaaattct cgatggtact
     5401 cctgttggag aattggtggt ggtcttgtca ggtgaaaaag cagcgttggc aggtgcccaa
     5461 gaagccattc gtcaagcagg tgtacaacta aaagtattga agggagtaca gtaagatgga
     5521 atcattgatt caaacctatt taccaaatgt ctataaaatg ggttgggctg gtcaggcagg
     5581 ctggggaacg gctatctact taactcttta tatgacagtt ctttccttca ttatcggagg
     5641 cttcttgggg ctagtggcag gtctctttct cgtcttgaca gcgccaggtg gtgtcttgga
     5701 gaataaagtc gtattctgga ttttagacaa aattacctca atttttcgtg cggttccctt
     5761 tatcatcctc ttggcaatct tgtcaccact ttctcacttg attgttaaaa caagtatcgg
     5821 gccaaatgca gcccttgtcc cactttcttt tgcagtcttt gccttctttg cccgtcaggt
     5881 gcaggttgtc ttggctgaat tggatggcgg tgtcattgag gcggctcaag cgagcggagc
     5941 gactttctgg gacatcgtgg gtgtttacct atcagaaggt cttccagatt tgatccgtgt
     6001 gacgactgtg accttgattt cccttgttgg ggaaacagct atggccggtg cggttggagc
     6061 tggtggtatc ggtaacgtag ccatcgctta tggatttaac cgctacaatc acgatgtgac
     6121 catcttggca accattatta tcattttgat tatctttgca atccaattct tgggagattt
     6181 cttgactaag aaattgagcc ataaataaaa aagagccgcg tggctctttt taactgatca
     6241 gattttctgt gcaaattttt tactcaaggc ttgtccaatc aaggcaccca ctagggctcc
     6301 gatgacaata cttgcgataa atagaaggac agttccaggg tttggagcga ccatgatacg
     6361 gtcgatatat tcttgggatt ttcctcttgc cagaagagta gccatatagg ctttgggcgc
     6421 aatccacata agcaagattg gtcctgttgt actaaaggcg aaaataatga aagaaaggaa
     6481 gttctttgtt ttgtccttgt attttcctaa atgagctact tcatctgcta ggaggccaca
     6541 gataattcca ggaaggaagg aaccagcacc gtgtttagtt cccaagaaaa agagggcaat
     6601 gacaaggccg atagtggtaa tggctccaaa gcgcggaact tttgcgacta ggatcatata
     6661 gacgctaccg ccgacaaggg cagtaaaggc aggcgcataa aacatgtttc cagtttggtc
     6721 aaagagattg cccaaaagga caccaatccc catgcagagg aagtaaagaa ctgcaaaaag
     6781 cagtgtagtt aagatagatt tcttcatgaa ttgtctcctg ataatttttt cacaattctc
     6841 attgtaccac ggtttggtgg gattgtaaat ggatactaga agtttttgaa aacgcttgaa
     6901 atatgatata atggtttcat agttattttt aggggaatat catgggtaga tttttagact
     6961 ttgtctttaa tcgtttcttt ttagggatga ttgcgacagc cttcttttgg ctattaactt
     7021 tagcaggagg gattatcctt ggtctagcgc cggctagtgc caccttgatg agcttatatg
     7081 cagaacatgg ttatagcttt cgggaataca gtttgaagga ggcttggtct ctttacaagc
     7141 aaaattttgt ctcaagcaac ctgattttct atagcttttt aggtgtgggt ctagttttga
     7201 cctatggttt gtatctcttg gtgcaattgc ctcatcagac cattgttcat ttgattgcga
     7261 cccttttgaa tgtcctagta gttgccctga tctttttggc ttatacagta tctttaaaat
     7321 tacaagttta ttttgccttg tcctatcgaa atagtctcaa attatccttg attggcatct
     7381 ttatgagtct agcagctgtg gccaagattc tccttgggac tgtgctactt gtagcaattg
     7441 gttactatat gcctgccctg ctattttttg taggaattgg gatgtggcat ttctttatca
     7501 gtgatatgtt ggaacctgtc tatgaaatca tccatgaaaa attggcgaca aaatagaatg
     7561 aagcagtttt ggctacatac gcttctaaga acctatagtt cagtgatgat cattatcatt
     7621 gcgagttttg caatcttact ctcttacgct gactgggatt cacgtgaaaa ggaagcccag
     7681 agagtagccc agcgtgtaac tgctagaaca gtgagtgaaa ttgaatatta ccatagagag
     7741 tcaacccaga tagctcaggc tttagttgaa aaccaagctc gtattgaggg aatctataaa
     7801 tactttagtc ttagcatgcc agactatttt tactggcaat tagagcggaa agcttcgcct
     7861 tatatatcag tctctctgta tgaaaatgtt gatgacctct atgttcgaaa tgattttgta
     7921 actggagtgg ccattgcttt tcaagattac aaggaagtct atgtttctac taaagacaaa
     7981 cgtagtggag aaaaaatcag ggctgaggat ttcaaaccag caggaaatag ttttgccatt
     8041 ccggtgtcag atccagtgtc agatcaagat ttaggagtga tttacatctc cttgaatcct
     8101 gctgttttat accatgccat tgataatact agaggtcata ctccgatggc agtaacagtg
     8161 acctcacctt ttgatacgga gatttttcat attggtgaga cagttgatag ggagagtgaa
     8221 aattggctag ttggcttaac ttctcatgga tatcaggttc aggtggcagt tcctaaaaac
     8281 tttgttttac aaggaacagt gactagctct gctttgattg tgggtttgag ccttctcttt
     8341 attgtcattc tttatctgac tttgaggcag acttttgcta attaccaaaa gcaggtagtg
     8401 gatttagtgg aatccattca agtcattgct caaggcgaag aggggcgtcg gattgacatt
     8461 tccgaaaaag atcaggaatt actcctaatc gcggagacga ccaatgatat gttggatcga
     8521 ttggaaaaga atatccatga tatttaccag ttagagctta gtcaaaaaga tgccaatatg
     8581 cgagccttgc aggcgcaaat caatcctcat tttatgtata atacgctgga gttcttgcgc
     8641 atgtatgcag ttatgcagag tcaagatgag ttggcagata tcatttatga attcagtagt
     8701 ctcttgcgta acaatatttc cgacgaaaga gagaccctcc tcaaacagga attagaattt
     8761 tgccgtaaat acagctatct ctgcatggtt cgctatccca agtccattgc ctatggtttc
     8821 aagatagatc cagagttaga gaatatgaag attcccaagt ttaccttgca accgctggta
     8881 gaaaactatt tcgcgcatgg tgttgaccac aggcggacag ataatgtgat tagcatcaag
     8941 gctcttaaac aggatggttt tgtggaaatt ttggtggtcg ataatggtag aggaatgtcg
     9001 gctgaaaagt tggcaaatat ccgagaaaaa ttaagtcaga gatattttga acaccaagcc
     9061 agctacagtg atcaaaggca gtctatcggg attgtcaatg tacacgagcg ttttgtgctc
     9121 tattttggag accgctatgc cattactata gagtctgcag agcaagccgg tgttcagtat
     9181 cgtattacaa ttcaagatga gtagaaaggg agaaaatgta taaagtatta ttagtagatg
     9241 atgagtacat ggtgacagaa ggtctgaagc gtttgattcc ctttgataag tgggatatgg
     9301 aggtcgtcgc aacagtcagt catgccgatg aagctctaga atatgttcag gaaaatcctg
     9361 tcgatgtcat catttccgat gtcaatatgc cagacaaaac agggcttgat atgattcggg
     9421 agatgaaaga gatcttacca gatgctgcct atatcctgct ctcaggttat caggagtttg
     9481 attatgtaaa aagagcaatg aaccttagtg tggtggacta tttggtcaaa cctgttgata
     9541 aggtagagct gggaaatctg ctggagaaga ttgcaggtca gctcggcgag agagggaaga
     9601 aaagtcagac tcttagtcaa gaattagacg aggctgaatt tgttagttat ttaggggata
     9661 aggagaattg gtggataggt ctatccaagg aaaaacaagg ttccttcacc attccctact
     9721 atgtcttggg ccaggactgg cagattttca tttctgacca acccctagat ggtttagtcg
     9781 ttacaccttt tgaagctcct tatcaagaac actttgaacg ctggaagctg aatgctgaga
     9841 aaaccctctt ttacggttct gtaaatttgc agcagtctga gagtctcttt gcctattacg
     9901 aaccgattta tagggttatc attcagggaa atctcaatca aatcgtagaa gagttaaatc
     9961 tcttggagaa ggtagttctt gaaaatacgc cgcgtgttcc gattactaaa cagcttttta
    10021 ttcagtttgt catggatgtt ttccatttat ttgaacatct caaagctgat gacctgacag
    10081 acatcgtcaa aaccattcat gctattcaat ccttcgatga attggttcct tatatcaagg
    10141 aaactctgac cagctttttc ggtcaatacc gtatgaatga aaatgtggtc agtgtgctgg
    10201 aagtcattgg tcgtgattac caaaaagagc tttccctcaa ggatatcagt aaggatctct
    10261 ttatcaatcc tgtctatcta gggcagttga ttaagcgtga aaccgattcg acttttgcag
    10321 agttactaaa caaacaacgt attaaggctg cccaacaact tttgctttca actagtgaca
    10381 gcatcgaaga tatttgttat gctgttggtt acagtaacct tggatatttc tataaagttt
    10441 tccgaaaatt gtgcggaaaa tcgccaaaag cctaccgaaa acaggtagaa actatactat
    10501 aagatttgta ttcctttaca aaatgtgcta taatataaat attattatca ggaggttcta
    10561 tcatgaaaaa gaaaccaatt tatctatggg tcttgctaat cttgtctgcc cttatttcag
    10621 ttccgtctct gtttggaatt gtgagtccct tgcctagtaa agaagccctt cgtactgctc
    10681 agaaacaagt tgcaggggtc aatgctcagc aattagaaga ccagcttaat tatacctata
    10741 gagtagcaga agcatctcat tctattttta atgttgcttt gattgtgcta tctactattt
    10801 tagtggtcgt agcgattgtc ttccttgttc gtaaaaattt gcaatatgca aactatactt
    10861 acgttggcta tgttttgtta gctattattg gttcgattta tggctatgtt ggtttacaag
    10921 acgctgtgca gttggttcaa gatgagagca tgcgtttgac agtgagtatt ggttcaaaag
    10981 ctgttagcat tttctatatc gtcatcaatg ttctcttcct agcccttgtc ttctataaga
    11041 tgtggcgaca acagaaagcc ttggcagaag aagaggaaac agaagagctt acctaagtat
    11101 tgacaaaaaa gaactatcaa gatacaatct tggtagttgt tttttgatta tagataaaat
    11161 catggaggta catatgaaga caatttcttt agtttatatt agtttgagtg gcaacactga
    11221 aagttttgtg acgcgcttga aagactatct cttgtcccag tacaaaggga ttgaggttca
    11281 aaagattcat atcaaggatt tggtcaagga aggcaaaaat ttctatgaaa tggaccatcc
    11341 ctatgtcgct tttttgccga cctatctcga aggtgggaat ggcgtggata acggagatgt
    11401 tgagattttg acgacaccgg tgggagattt tattgcctat ggtaacaatg ctagtaagtg
    11461 ttttggtgtg gttgggtcag gaaatcgtaa ctttaataac caatactgcc taacagccaa
    11521 gcaatacagt caacgctttg gcttccctgt attggctgac tttgaaatgc gaggcatgct
    11581 ggaagatatc aaacatgtcg cagctattat cgcagatttg tatgagttgg aaaaggagaa
    11641 ttaatactca atgaaaatca aagagcaaac taggaagcta gtcgcaggtt gctcaaagca
    11701 cagctttgag gttgcagata gagctgacgt ggtttgaaga gattctcgaa tagtataaag
    11761 ttcttataag atgtggttga agttgttcaa ctgttttttg agtataaaca gtctttaaga
    11821 acgtaaaaaa gcataagttc aggtcttcaa aaccttgttc ttatgctttt tatcatagaa
    11881 atatttgctg aattttctct tgaaatgaaa ccttatcttc atctcgcgct tgtcccttac
    11941 cctgcttttc cgattgcgag tgcgtagata aagaagatgg cgaagccaaa gaggaaaatc
    12001 aatcccgcaa tggcaaggtg tttgagccaa gaaggaagat ttttgttttc acgtgttacc
    12061 aaggcgtaat tcaaaagagc aaagaaaggt gttgtcagga aagagccaat catggcaaag
    12121 cggagcatgg ttgaaacctg accagcgaag aacttgataa tgacgatacc gatgatagca
    12181 gtgatggtca tccagatgtt caaagattta cgattgtcct ctttttgact gattagcagt
    12241 cggagagatt cctgattaac gcgagaatag ccatcgataa cagttataac tgttccaaag
    12301 atacagagga aggcaataaa ggtaatcaag taacgggacc attcgccaag aacagaggca
    12361 tacatgccca cgaattgaga gatgtatttg gctgaagcag cttcaaccgc ctgccctgta
    12421 ggatactgaa tcagtgctcc cagtgccaca aagaagacgg ctaggatagc tgttccaata
    12481 taaccagtgt taaagtcaaa cagagcgtct tctgtgttaa agttgacggt ctttctcttt
    12541 tcagctgacc aaagtgaatt gatggctgaa atttcaatag gagccggcat ccatcctagg
    12601 agggagacga tgaagggcag agctgccatt tgccaaggtg tcttctcgac aaaatcagaa
    12661 ctgtattctg gatgcttgac cgccgcaatg ataactgcaa gaacagtcgc aatggttaag
    12721 gcagacataa tccatttgac catgccgtct aaaagtttgt agcctccaaa gagtagcata
    12781 gcccaaatga ttgcaacgag aatgagggac cactgagtaa tgctaagtcc aatcattggg
    12841 aaggcactgg cgatgatagc tgagcacaga atggcaacac cagccgtgtt gaccatagcc
    12901 gaaaagacat tgagaataaa gaaaatccag agatagagtt ttcctttttc ggcataacct
    12961 tcaaccaaag tctttccagt atcagctgtg tattcagcac caaaacggaa aaatggatat
    13021 ttaaagacat tggctaagat gaccaagaga agtagagacc aaccgtaaga accgccagct
    13081 tgagttgagg atacaatgtg ggaacctcca acagcggcag ttgccattag gattccaggt
    13141 cccattgctt ttagcttgct tgcccaggtt gattgattta aggaaatagc ttgcgacatg
    13201 ataaatactc ctttttgaat tttcagaata atctgtatat gcaatctata ttctacaaaa
    13261 aattttggaa aaatgcaaga atattttgaa aaaagataga aaatttacaa agaaaatctg
    13321 aaataactgt tatgatatag cagattgaaa ctagaatagt acacttttgc ttctaaaaca
    13381 ttgttagaaa tcgatttgac tgtcctgatt gatttatcct gttcttattt catttcacta
    13441 tagtaaggtg cagcaggata aagcaaaacc gagaagtttc tttctcggtt ttgcttatta
    13501 tgaggttcct attttctatg ctccaagagt tgattgatgt gatctacttt tttagcctct
    13561 cttttataga gaatagccca gatgatgaga tagacaatcg caaactcgat aatgagctgg
    13621 aggtagaaaa tccagtggaa ggggaaccaa ccagcaagag ttgctagtgg gacaaagcca
    13681 gccagcataa ggaagaaatg agtcagagtg gcacgaagca tgctccagtc acggctgaat
    13741 aagcggttgc caaagttgaa gagcataccg atggctgccc agataagtgt gcagtagagc
    13801 aagaccaggg caccgtgaac ctgatgctga tccatcactt ggccgatgag agagtaggga
    13861 tttagtggtg cgtaggtatt tggtgcataa atgagtgaaa agaggatgga gaggatgagg
    13921 ccgataagaa caccggcagc tgcatcgtga aatacttgtt ttttcatagt tctaatttct
    13981 ccttgatggt ttttagataa cggcgtgaag agtaggtgaa gctttcgttt ttcaagaaaa
    14041 tttctaccag accgtttggc gtgagcttga ggtgagagat ggaatcgata ttgatgattt
    14101 ctgattggga aatttggata aaattgcttg gcaagagttt aagaacctga tagagtcgca
    14161 aatcaatgct gtaggtctga ctcgcggttt ctgctagaac cttccgattc tcgatataga
    14221 agcgctgaat cttgccaatc tcaactagat agacctgatc atcgattttt cctttgattt
    14281 tttctctttg gtccagattt tctgcgaact cgatgacttt ctggactttt tcggtttctt
    14341 gaggtgcttg gacaatcagc ttttcctcct cgtaagtctc actaatctgt agttctactt
    14401 tcataaattt ctctccttgt ttttcataca aaattaatct cacttcttgt acacctttat
    14461 taatactctt caaaaatctc ttcaaaccac gtcagcgtcg tcttgccgta ggatagttgt
    14521 tgactttgtc agttttatct acaacctcaa aacagtgttt tgagctgtct gcgtctagct
    14581 ttctagtttg ctctttgatt tttattgagt ataccactat tttactccct ctggcaaggg
    14641 actttgtcta tgtggaggga ttgggctcct atgtggtgga gtttttctgt tctttctgaa
    14701 atatggtata atagcactaa ttaatttcta ggaaaataga tacagaaagg ggctgaaaga
    14761 tgtctcatat tattgaattg ccagagatgc tggcaaacca aatcgcagct ggggaggtca
    14821 ttgaacgtcc tgccagtgtg gttaaagagt tggtagaaaa tgccattgac gcgggctcta
    14881 gtcagattat cattgagatt gaggaagctg gtctcaagaa ggttcaaatc acggataacg
    14941 gtcatggaat tgcccacgat gaggtggagt tggccctgcg tcgccatgcg accagtaaga
    15001 taaaaaatca agcagatctc tttcggattc ggacgcttgg ttttcgtggt gaagccttgc
    15061 cttctattgc gtctgttagt gtcttgactc tgttaacggc ggtggatggt gctagtcatg
    15121 gaaccaagtt agtcgcgcgt gggggtgaag ttgaggaagt catcccagcg actagtcctg
    15181 tgggaaccaa ggtttgtgtg gaggatctct ttttcaacac gcctgcccgt ctcaagtata
    15241 tgaagagcca gcaagcggag ttgtctcata tcattgatat tgtcaaccgt ctgggcttgg
    15301 cccatcctga gatttctttt agcttgatta gtgatggcaa ggaaatgacg cggacagcag
    15361 ggactggtca attgcgccaa gcaatcgcag ggatttacgg tttggtcagt gccaagaaga
    15421 tgattgaaat tgagaactct gacctagatt tcgaaatttc aggttttgtg tctttacctg
    15481 agttgactcg ggctaaccgc aattatatca gcctcttcat caatggccgt tatatcaaga
    15541 acttcctgct caatcgtgct attttggatg gtttcggaag caagcttatg gttggacgtt
    15601 ttccactggc tgtcattcac atccatatcg acccttatct agcggatgtc aatgtgcatc
    15661 caactaagca agaggtgcgg atttccaagg aaaaagaact gatgaccctg gtttcagaag
    15721 ctattgcaaa tagtctcaag gaacaaacct tgattccaga tgccttggaa aatcttgcca
    15781 aatcgaccgt gcgcaatcgt gagaaggtgg agcaaactat tctcccactc aaagaaaata
    15841 cgctctacta tgagaaaact gagccgtcaa gacctagtca aactgaagta gctgattatc
    15901 aggtagaatt gactgatgaa gggcaggatt tgaccctgtt tgccaaggaa accttggacc
    15961 gattgaccaa gccagcaaaa ctgcattttg cagagagaaa gcctgctaac tacgaccagc
    16021 tagaccatcc agagttagat cttgctagca tcgataaggc ttatgacaaa ctggagcgag
    16081 aagaagcatc cagcttccca gagttggagt ttttcggaca aatgcacggg acttatctct
    16141 ttgcccaagg gcgagatgga ctttacatca tagatcagca cgctgctcag gaacgggtca
    16201 agtacgagga gtaccgtgaa agcattggca atgttgacca gagtcagcag caactcctag
    16261 tgccctatat ctttgaattt cctgcggatg atgccctgcg tctcaaggaa agaatgcctc
    16321 tcttagagga agtgggcgtc tttctagcag agtacggaga aaatcaattt attctacgtg
    16381 aacatcctat ttggatggca gaagaagaga ttgaatcagg catctatgag atgtgcgaca
    16441 tgctcctttt gaccaaggaa gtttctatca agaaataccg agcagagctg gctatcatga
    16501 tgtcttgcaa gcgatctatc aaggccaatc atcgtattga tgatcattca gctagacaac
    16561 tcctctatca gctttctcaa tgtgacaatc cctataactg tcctcacgga cgtcctgttt
    16621 tggtgcattt taccaagtcg gatatggaaa agatgttccg acgtattcag gaaaatcaca
    16681 ccagtctccg tgagttgggg aaatattaaa agtataaaaa agtctgggaa aaattttcaa
    16741 aatcagaaaa acgcataaaa tcaggtgttc aaaaaccttg attttatgcg ttttatcatg
    16801 gaaatagtta cttcatgttt tcctaattct tttcgaaact ctttttaaac cacgtcagtt
    16861 ttatcagtaa tctcaaaaca gtgttttgag ctaattttgc cagttttgtc tgtaacatcg
    16921 aagttgtgtt ttaccactct gcgactggtt tcctagtttg ctctatgatt ttcacagagt
    16981 attaaattgc gattttgcca agtttcttta ttcgtctaaa agtagagtct gttctatgcg
    17041 tctaatgtac gaattaggtt gaccatttca atagctcctt gtgcacactc agaaccctta
    17101 tttcctgctt tggtaccagc tcgttctatg gcttgttcaa ttgtatctgt cgttagcaca
    17161 ccaaacataa caggaatttc gctatttaaa ctgatttggg cgattccctt agatacctcg
    17221 ctacatacat aatcataatg acttgtattc cctctaatga cagctcccaa gcagataatt
    17281 gcatcatatt ttttactttt tgccattttt gatgcaatca gtggtatttc aaaagctcct
    17341 ggaacccagg ctacctctat atctttctcg tttacattct ctcttttgag attatctagt
    17401 gctccagata ataattttga agttataaat tcattaaatc tcgctacaac aatacctatt
    17461 ttaatattgt ttgctactaa attaccttca taagtgttca tttatttttc ctccatattt
    17521 aaaatgtgac ccattcgatt tttctttgtt tctaaataaa aactatcgta aggattggct
    17581 tctatttcga ttgatattct actggaaatg gtaattccat atttttctaa ctgttcaacc
    17641 ttgtcaggat tatttgtcag taaatgaagt gactgaagtc ccagatcttt aagcatttgt
    17701 gctccaatat gatattctct taaatcacct tcaaagccta atgcaagatt ggcatcaagc
    17761 gtatccatgc cttgatcttg taaatgatag gcttttaatt tattgataag tccaattcct
    17821 cgtccctcct gtcgcaagta aagtaagaca cccgaaccat tctcaacaat tattttcata
    17881 gctttatcga attgctgtcc acaatcgcaa cgtaaagagc ctaaaacatc tcctgttaaa
    17941 cattcggagt ggacccgaca taatacattg gcttcatcct ctatgtttcc cataataaga
    18001 gcaagatgat gttccccatt tagtttatct atatagctaa ttgctttgaa attaccgtat
    18061 ctagtaggca tattgacagt tgaaactcgt tctaccagct gatcatatgc ttttctatat
    18121 tcttgtaatt ctttgatggt aattagtgga atgttgtgtt tcttcgagaa ctgaattaaa
    18181 tcatctattc tcatcatttt gccatcatga ttcattattt cacaacatag gccacactct
    18241 tttagtccag ctaattttaa taaatcaaca gttgcttctg tgtgtccatt tctttctaga
    18301 acaccacctt tttttgcaat taaaggaaac atgtgtcctg gcctgcgaaa atcagagggt
    18361 gttatatctt cagctacaca catacgtgcg gtcagtcctc tctcctcggc agaaatacct
    18421 gtggtcgttt ctttataatc aattgaaact gtaaaagcag tcttatgatt atctgtattg
    18481 ttttcaacca taggtgaaag cattaattga ttagctaaac tttcgctcat aggcatacaa
    18541 attaatcctt tggcataagt agccataaaa ttaacatttt ctgttgtagc tgcttgtgca
    18601 gaacaaatta agtctccttc attttctcta tccttgtcgt ctataacaag aacaagtcgt
    18661 cccttctgca atgcttctaa tgcttcttgt atttttcgat attccattga ctgattatcc
    18721 tttctgctaa aatccatttt gatataatag ttccttcgat atttctgatt ttggagagtt
    18781 atccatcagt ttttgcacat atttacctaa gatatcattt tcaagattta ctgtactccc
    18841 gacttgttta ctcttaagaa tggtttgttt caaggtatga gggataacag atactgaaaa
    18901 gtttactttg gagactttag cgacagtcag actaatgccg tcaattgtaa tagatccttt
    18961 ttcaactatt aaatctaaaa tttctttttg tgtgttgatt tgataccata cagcattatc
    19021 atcttttttt attgacgaga tttttcctgt accatcaatg tgtcctgtaa cgacgtgacc
    19081 cccaagtcga ccgttgacag ataaggctct ttctagattc acctcacttc catgttttaa
    19141 tagagtaaga gctgttcgac tccatgtttc attcattaca tcaactgtaa aggattgatg
    19201 attgaaatga gtaactgtaa gacagatacc atttactgct atactatcgc ctaaatggat
    19261 atccgttaat atttttgagg ctttaattga tagtttacaa ttacgagagt ctttctgtat
    19321 tctttcaact tttccgattt cttcaattat tcctgtgaac atggataaat cacttcactt
    19381 tctatgagat agtcatttcc tatttgagaa aaagcataag gtttcaatct aatagcgtca
    19441 tttggcaaag aaatgccttc acctccgaca ggaaacttgg cactgcctcc aaaaattttt
    19501 ggtgcaatat atattttcag ctcatcaaca atttgttgtt ccaaagcact ccaattcatt
    19561 agactgcccc cttctagaac taggctatca atctgcatgt ttcctagatg ttgcattaaa
    19621 ctcgataagt ctatatgatt gccttttttc tttatggaaa gtatttcaca gccatgattt
    19681 tgatatagct tcattttatt tttgtcttca gaggaagtgg caatgtaagt tttaatatca
    19741 tttgctgttt ttacgatttt agaggtaaga ggagttcgta aatgtgtatc gcatatgata
    19801 cggataggat ttttcccttc ctccaatcta catgtcagca aaggatcgtc ttgaataaca
    19861 gtattgactc ccaccataat tgcactaaca tggtgtcgta actgatgcac atgctttctt
    19921 gcctcttctt cagtaatcca tttggattga tttgttttag tggctatttt tccatcaatt
    19981 gtcattgcat atttcataaa aacatagggt acatgctggg taatatactt tctaaaactt
    20041 tttattaagt taagacactc attttctaaa attccaacag taacttgaag attattttcc
    20101 tcaagtatct ttactccttt tccagataca ataggattac agtctaggct tccaatgact
    20161 actcttgtaa taccactatc gattatagca tctatacaag gaggtgtttt cccgaagtga
    20221 caacagggtt caagtgttac ataaagcgtc gctccgacag gggattctct acagttttta
    20281 agagcatttc tctcagcatg tgagccacca aaaaactcat gataaccttg tccgatgatg
    20341 tgattatctt ttacaataac tgcgccgacc ataggattgg gattgacgta accagcccct
    20401 ttttgtgcca gttttattgc taatttcata tattttgaat cgctcatctc actacctcca
    20461 aaaaaatata ccttgaatag gagactactc aaggcataca aaagaaaact tatgcgatta
    20521 acaaaaatgc tctgaaatga caagtaatca tttcagagca cgcaaaaagc acaaatatac
    20581 ttttatcttc tttcatccag actatactgt cggctttgga atttcaccaa atcatgcctt
    20641 tcggctcgtg ggctatacca ccggtaggga attgcaccct gccctgaaga tagttattca
    20701 attacagatg attatagtac ttaattttga atatgtcaac agataaatac cgattgtttt
    20761 tgatatactg tatttgtgat aatcgattct cgctcctcgg ataaagaaaa tatgatatac
    20821 tagataaacg aaataagaga gaaggaacac tatgtacgca tatttaaaag gaatcattac
    20881 caaaattact gccaaataca ttgttcttga aaccaatggt attggttata tcctgcatgt
    20941 ggccaatcct tatgcctatt caggtcaggt taatcaggag gatcagattt atgtgcatca
    21001 ggttgtgcgt gaggacgccc atttgcttta tggatttcgc tcagaggatg agaaaaagct
    21061 ctttcttagt ctgatttcgg tctctgggat tggtcctgta tcagctcttg ctattatcgc
    21121 tgctgatgac aatgctggct tggttcaagc cattgaaacc aagaacatca cctacttgac
    21181 caagttccct aaaattggca agaaaacagc ccagcagatg gtgctggact tggaaggcaa
    21241 ggtagtagtt gcaggagatg accttcctgc caagatcgca gtgcaagcaa gtgctgaaaa
    21301 ccaagaattg gaagaagcta tggaagccat gttggctctg ggctacaagg caacagagct
    21361 caagaaaatc aagaaattct ttgaaggaac gacagataca gctgagaact atatcaagtc
    21421 ggcccttaaa atgttggtca aataggagca gagaatgaca aaacgttgtt cgtgggtcaa
    21481 gatgaccaac ccactctaca tcgcctatca tgatgaggag tggggccagc ccctccatga
    21541 tgaccaagcg ttgtttgagt tgttgtgtat ggaaacctat caggcaggcc tgtcttggga
    21601 aacggtactc aacaaacgcc aagctttccg agaagccttt catagctatc aaattcactc
    21661 agtcgcagag atgactgaca ctgaattgga agccatgctg gagaatccag ctatcattcg
    21721 aaatagagcc aagatttttg ctacacgcgc taacgcccaa gcctttctac agttacaggc
    21781 agagtacggc tcttttgatg cctatctttg gtcttttgtt gaggggaaaa ctgtcgttaa
    21841 cgatgttcct gattatcgcc aagccccagc taaaacaccc ttatctgaga aattagccaa
    21901 agatctcaaa aaacgagact tcaagttcac aggcccagtc gccgtattgt cttttctaca
    21961 ggctgcaggg ctagttgatg accacgagaa tgattgtgag tggaaaggtc ttaaatgatg
    22021 tctaacaaaa ataagggaat tctgattttt gcgattctct atacagtcct ctttgtgttt
    22081 gatggcgtta aattgctggc ttctttaatg ccatctgcca ttgcaaatta tcttgtttat
    22141 gtagttttag ctttatatgg ctccttcttg ttcaaggata gattgatcca acaatggaag
    22201 gagattagaa agactaaaag aaaattcttc tttggagtct taacaggatg gctctttctc
    22261 attctgatga ctgttgtctt tgaatttgta tcagagatgt tgaagcagtt tgtgggacta
    22321 gatggacaag gtctaaatca gtctaatatt caaagtacct ttcaagaaca accactactg
    22381 atagctgttt ttgcttgtgt cattggacct ctggtagaag aattattttt ccgtcaggtc
    22441 ttattgcatt acttgcagga acggttgtca ggtttactaa gcattattct ggtaggactt
    22501 gtttttgctc tgactcatat gcacagtttg gctctatcag agtggattgg tgcagttggt
    22561 tacttaggtg gaggccttgc cttttctatt atttatgtga aagaaaaaga gaatatctac
    22621 tatcccctac ttgttcacat gttaagcaac agcctctcct taatcatttt agctatcagt
    22681 atagtaaaat gaaat
//
LOCUS       DAVAAL010000020        22666 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVAAL010000020 DAVAAL010000000
VERSION     DAVAAL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22666
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.020"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(371..1351)
                     /locus_tag="VQM60_000640"
     CDS             complement(371..1351)
                     /locus_tag="VQM60_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET1080530.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1668..3683)
                     /gene="recG"
                     /locus_tag="VQM60_000641"
     CDS             complement(1668..3683)
                     /gene="recG"
                     /locus_tag="VQM60_000641"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET1080531.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLI
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLALIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3702..4805)
                     /locus_tag="VQM60_000642"
     CDS             complement(3702..4805)
                     /locus_tag="VQM60_000642"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET1080532.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4795..5163)
                     /locus_tag="VQM60_000643"
     CDS             complement(4795..5163)
                     /locus_tag="VQM60_000643"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET1080533.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5202..6233)
                     /locus_tag="VQM60_000644"
     CDS             complement(5202..6233)
                     /locus_tag="VQM60_000644"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET1080534.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6235..7266)
                     /locus_tag="VQM60_000645"
     CDS             complement(6235..7266)
                     /locus_tag="VQM60_000645"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET1080535.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7347..9860)
                     /gene="secA"
                     /locus_tag="VQM60_000646"
     CDS             complement(7347..9860)
                     /gene="secA"
                     /locus_tag="VQM60_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET1080536.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRK"
     gene            complement(10006..10293)
                     /locus_tag="VQM60_000647"
     CDS             complement(10006..10293)
                     /locus_tag="VQM60_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080537.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10304..10954)
                     /locus_tag="VQM60_000648"
     CDS             complement(10304..10954)
                     /locus_tag="VQM60_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET1080538.1"
                     /translation="MKLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(10958..11599)
                     /locus_tag="VQM60_000649"
     CDS             complement(10958..11599)
                     /locus_tag="VQM60_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080539.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEVNLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLFAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11592..12383)
                     /locus_tag="VQM60_000650"
     CDS             complement(11592..12383)
                     /locus_tag="VQM60_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080540.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIVTYFFGTFSPLGWNSLFSDGSSLQR
                     LLDGEITSYLFFTCVLLIGIFINAVYFFKIVDYLGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(12568..12903)
                     /locus_tag="VQM60_000651"
     CDS             complement(12568..12903)
                     /locus_tag="VQM60_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080541.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     SLFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGVRH"
     gene            complement(13043..13378)
                     /locus_tag="VQM60_000652"
     CDS             complement(13043..13378)
                     /locus_tag="VQM60_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080542.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13469..14779)
                     /gene="der"
                     /locus_tag="VQM60_000653"
     CDS             complement(13469..14779)
                     /gene="der"
                     /locus_tag="VQM60_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET1080543.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14793..15506)
                     /locus_tag="VQM60_000654"
     CDS             complement(14793..15506)
                     /locus_tag="VQM60_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET1080544.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15503..16399)
                     /gene="dnaI"
                     /locus_tag="VQM60_000655"
     CDS             complement(15503..16399)
                     /gene="dnaI"
                     /locus_tag="VQM60_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET1080545.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16400..17569)
                     /locus_tag="VQM60_000656"
     CDS             complement(16400..17569)
                     /locus_tag="VQM60_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET1080546.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRRLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17570..18043)
                     /gene="nrdR"
                     /locus_tag="VQM60_000657"
     CDS             complement(17570..18043)
                     /gene="nrdR"
                     /locus_tag="VQM60_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET1080547.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            18183..18548
                     /locus_tag="VQM60_000658"
     CDS             18183..18548
                     /locus_tag="VQM60_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET1080548.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18553..19248
                     /locus_tag="VQM60_000659"
     CDS             18553..19248
                     /locus_tag="VQM60_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080549.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19265..20032
                     /locus_tag="VQM60_000660"
     CDS             19265..20032
                     /locus_tag="VQM60_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080550.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20385..21584)
                     /locus_tag="VQM60_000661"
     CDS             complement(20385..21584)
                     /locus_tag="VQM60_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1080551.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21581..22474)
                     /locus_tag="VQM60_000662"
     CDS             complement(21581..22474)
                     /locus_tag="VQM60_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080552.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
BASE COUNT         6656 a         5095 c         4213 g         6702 t
ORIGIN      
        1 cggcaaggcg aagctgacgt ggtttaaaga gatttttgaa aagtattaac acctacagac
       61 aagcctgacg atagcaagaa ctaccctact cgataggtat cggcttttgc tttctgaaaa
      121 aaattatttt aagcaatttg acaaatctag caacaaaaaa ttctataaat ataatagatt
      181 gaaactagaa tagtacacat ttgcttctaa aacattgtta gaaatcgatt tgactgtcct
      241 gatcgttttg tcctattctt gtttcatttt actatatttc catgataaaa cgcatagtat
      301 caagtttttt taatcccctg atactatgcg tgtttgtaat ttttaagatt ttgtgcttag
      361 agtcgactcc ttattttaga tatttaaaag gaatctcact tccacagagc cagttgtaga
      421 cttggtcatt gacaaataca ttcatggctt cgtgagcata ctcaggcatg atgcgatagg
      481 ttttatcgca ggtcagacga ttataaatcg caaactgggt aatgggatag caaacatcgt
      541 cgtccaagcc cgtaatcatc ttaacctcac cttggatacg atgggcaaga tttttgacat
      601 cgatataggc aagggtcgcc atgatttcct cctctgtttc atggaagggg tcgtgaaact
      661 tgaaataacg gaaaagttcg tcgtaagcct cgctagtatt accaatctca atcacccgtc
      721 tgaagtctga caagaaggga taaatggcaa ctgttttctg aattcgagga ttgagcgctg
      781 ctgcaactag agctagagcc cctccttgtg aggcaccata gctagaaaga cgcttctcat
      841 caacctgaga cagactagca acaatttcga ccaactggta aatatccaga taaacatcct
      901 tataaaagag gtggtcccga ccttccatag caccacggat aatatgcccc ttcacggtat
      961 ttcctaaagg agaacgcaag ccgtcttgtg agtaacctga ctggccccgc acatccatgg
     1021 aaacaacacc gtaaccagct acggtgaagc ccagcatgtc ggcccagtcc cagccacgtc
     1081 ccatataacc atgaaaatgg aagattaatg gaaccttctc ctcactcttt ggaagaacaa
     1141 tgcgtgcata gacctttcct tccttgcttc cttcaaatgt taactcatag cacttgactt
     1201 gaggaatgtg gaaatctctt tcctccaagt ggtaggatgg aagtgtggaa acatttttca
     1261 cttccccatc ccagaaagca tcaaagtctt ccggaacctc atcccttcct ctataggtct
     1321 taatttcttc tagcaaagct ggatttttca tagcatgctc cttttatttt gtaatcgtta
     1381 ccacaactgt agcatatcta tgtaatcaat gcaagaaaga agggtaaata gaaagtgaat
     1441 ttagattctt tacttttagg atgatgggct gaaagtagtt ttagaaacga aaaaagagca
     1501 tttcgctctc tcttctgttt atactagttt tttgcttcct tcttttggaa agtggtttgg
     1561 taatttactt taatcgttac tgtcatatga gagtcctcgt ttctttttga tgactttagt
     1621 ataagagcca aacctaaaaa ctagcttaag ttttccttag agaaagctta atccagatgt
     1681 tctttctttt ccagatgaag ggcaatcatg cgccactctg gatcttcttg ccaagcttct
     1741 atagaactaa tgtagctagc aacctttctt gcttcttcta aaatcggaaa atcttcgata
     1801 atatcagcca cttggaactc tggaagtcct gactgtctgg ttccaaaaat ctcaccagaa
     1861 ccacgcattt tcaaatcttc ctccgcaagg acaaatccat tggtcgtttc tgtcatgata
     1921 cgcatgcggt ctttcccaga atccgtcttg ggattagcaa cgagaacagc gtaggactgc
     1981 ttgtcccccc gaccgacacg acctctaagc tggtgaagtt gactgagacc gaagcgatcg
     2041 gcatccatga taatcatgac agtcgcattg ggaacgttga ccccaacctc aataaccgtc
     2101 gtcgaaacca gaatatccgt ctttctctcc ttgaaatcct gcatgatctg gtctttttcg
     2161 tcactcttca tcctaccatg tagaagagcc acctctgcct tgcctgcaaa atgagtcgtc
     2221 aactcctctg ataaggcaat ggcatttttc aaatctagag cttctgattc ttcaatcaaa
     2281 ggagagatga cataggcttg ggaacctttt tgaatttccc cctctaacca agtcaagacc
     2341 tgaggtagtt gctcatgttt gatccagcgc gtcacaatag gcttccgacc tgctggcatc
     2401 tggtcgataa tggaaacatc catatctcca aaggctgtga tggcaagcgt ccgtggaatg
     2461 ggagtcgccg tcatcatgag gacatctgga ttgtcacctt tttcccgtaa aatacgcctt
     2521 tgccctacac caaaacggtg ctgctcatcg ataataatca aagcaagacg agcatactcc
     2581 accccatctt gtatcagagc gtgagttccg ataatcaaat cagcctcacc cttggcaatg
     2641 gtctccaaga cctctctctt ttctgcagct ttcaaggaac ctatcaagag agccagtttc
     2701 aaattgggaa aaaggttctg taaactctca aagtgttgct ctgcgaggat ttctgttggt
     2761 accattaggg cagcctgata acctgctgtc actgccgcaa acatggccaa gccagcgact
     2821 accgtttttc cactccccac atccccttgt aggagacgat tcatgtggtg gtcggacttc
     2881 atatcagtta aaatttcctg caaactcttt tcctgagctt gggtcagggc aaaaggaaga
     2941 cttactttaa ctgctgtcac tttttcctga gaccaattca gaaccagacc acttccctga
     3001 actctatttt cagacttgag catctgcagc tgcatttgga aataaaagag ttcctcaaac
     3061 tttatacggc gaagagcctg cttgtattct gccaaatcct ttggaaaatg catagctcgg
     3121 actgcctgac aacgggacat gagtttgtat ttgtctagta aagactgggg cagattttct
     3181 tctatcaaga ggtccagtcc ctgatcaaaa gccgtcttga tgaccttgac cagactggcc
     3241 tgactgattc cctgagccag acgatagaca ggctggaggt catcttctac ctgagccaga
     3301 accttcatcc cagtcagact agccttagcg cggtcccatt ttccaaagac agcaagggtt
     3361 gctcccaact ctattttatc agccagatag ggctggttaa agaaattcac cgcaaaaacg
     3421 acctctccct gcttgagact aaaacgcagg cgattgcgct tgaaaccata atactggaca
     3481 ctagcaggag tcactacctg accagaaaga actgccttct caccgtcttc cagctccagc
     3541 acctgcttgg ttttgaagtc ttcataacgg aaaggaaagt agagcaagag atcttgcaag
     3601 ttttcaattc ctagtttggc gtatttttct gctgactttg gtcccacacc aggcaagaca
     3661 tgcaagggtt gatgtagatt catgctccac tcctttcttt tctaataata ttctctcgga
     3721 atacggtcgc tgaggaggca aaccacctca tagttaatgg ttacgcggta ggtcgctacc
     3781 tgagttgcag tgatttcctt atccccattg gagccaatca aggttacctt ggttcctagc
     3841 ggataaagct taggcaatcg aatagtgatt tggtccatcg aaaccctgcc gacaattggg
     3901 caagcttggc catctaccaa gacagagaaa ttttgcatgt ctcttgtcca tccatctgca
     3961 tacccgattg gcacggtcgc gatgacttgc tcgctatccg cttgataagt tgctccatag
     4021 cccatgcaag ctccagctgg aactgtcttg acatgaacca gagcagactc caaggtcaag
     4081 gccggtatca aatcataagg caaatccaag accgctccac ttggattgag gccatacatg
     4141 gcatctccca tacgaaccgc attgaaaata gtctctacat gccaaagagt cgttgcagaa
     4201 ttgctagcat gaaccagctc tggaacttcc ttcatactag ctaaaatagt tttaaaccgt
     4261 tctaactggg cattaaaata gtcatctgat tcctcatcag cagtagcaaa gtgggtaaag
     4321 attccttcaa cacaaacacc gtgttgttgg agcaaatctt gagcctgctc aacctcactt
     4381 gcctctctaa aaccaatccg tcccatccct gaatcaatct tgaggtggac tgtcaatcca
     4441 gttaggtcca cttccttatc taagagtgct tgaatccact ccagtccagc cactgtcaag
     4501 gtgaagtcat attctttagc tagagcaaca gcttcgattt cagaaactcc taaaatgagg
     4561 attggcttgc tgagtccagc ttgtctgagt tcaatggctt catcgatatt ggaaacgcaa
     4621 aagccatcaa catcatcttg aattgccttg gcaacggcaa cagctccatg accataagca
     4681 ttggccttaa ccacagccaa cttgagcgtt ccttgaggga tatgagcccc catttgctga
     4741 atattttgtc gaatagctcc cagatgaatc agagccttgg ttggtctatg tggactagct
     4801 ttcatgattt tcctccaaaa tgacactggc tgtcacaaac tgatcggtgt ggctgataga
     4861 cagccaaatc tttcctgaaa atggtgcctg actaaaataa ggcgccccac gttcattgtt
     4921 caagacttcc aaatcctgaa aaccgagctt gctaatgccc gttcccatag ccttggaaaa
     4981 ggcctcctta gccgaccagc gaccagctaa atattctatt tgcctgcgtc ctttgagact
     5041 ggtgaagcgc tccatttcct gagcggtcag tacacgctta gcaaatcctt catgtcgtgt
     5101 aactgcgctt tctatcgaag ccaattcttc gatgtcaatt ccgtgtccaa ctatcattct
     5161 catcaaaagg agttgagatt ccccaactcc atccttttca cttattttgt caaggtaaca
     5221 taaatctctt ctaccaaggc ctctgtattt tcccaaccta ggcaagggtc agtaatagag
     5281 caaccaaaga tctctggttg gttttgacga ccatctgcta ggtaagattc aatcataaat
     5341 cctcgaaccg tctttttaat tttctcattc caatcacgat tctgcaaggt ctggcgaaca
     5401 attcgaatct gctccatata ttgcttgcct gagttatcat ggttggtgtc aatgaggata
     5461 aaaggatttt caagtcccat ggtttcatag cgttcaatgg cttggagtag attttcatag
     5521 tagtaattcg gcatataatt gccatactcg ttgactgctc cacggaggat aacatgggcc
     5581 aaaggattac ctgatgtctc aacttcctgc ccatgataaa ggaaggtttg cttgttttga
     5641 gcagcataga tggcgttaaa cataacaccc aaatttcctg aggttggatt tttcatccct
     5701 actggtgcat caatcccaga agccacaaag cggtgctctt ggtcttccac agaacgagct
     5761 ccaacggcat ggtagctgac caagtcatcc accaagatca gatttgacgg ataaagcatc
     5821 tcatctgccg ttgtcaaacc agtctctgta atcacgcggt agtgcaactg gcgcacagcc
     5881 tgcaagccat taatcaggct tggagcctta gaagtatctg gctggtgaac taatccttta
     5941 tagccgtctc cattggtacg aggcttagca gtatacacgc gcatgaccat gaaaatcttg
     6001 tccgctacct tcttttgcaa ggcggataaa cggcgagcat attccaagac cgcctcttca
     6061 ttatcagaag agcaaggacc aatcaccaag agaatacggt catcttcccc tgaaataata
     6121 tctgccaatt ccctgtcacg gctttccttg agtctcaagg cttccgcaga caattgggtt
     6181 tcagccttaa tgacttccat atcgatttct tgaccttttt caataaatgc catcttattc
     6241 tcctaacgtt ttgtaaattt ctctgacaag agcttctgtg ttatcccaac ccaggcaagg
     6301 gtctgtgata gacttaccaa atacttctgg ctcattttgt cgaccatctt ccagataaga
     6361 ctcaatcata aaaccacgaa cgaactgctt aattttttca ttccaagcac ggttaatcaa
     6421 ggtctggcgg acaattcgga tctgttcaat atactgctta ccagaattgt catgattggt
     6481 atcaatgatg ataaaaggat tttccaagcc cattttctca tactgggcaa tggtatcaat
     6541 taaattgtca taatagtagt tgggaatatt ttttccatac tcattaagag caccacgaag
     6601 aatagcgtgt gaaagcgggt tcccagttgt ttctacttct tttcctaaga aaaggaaact
     6661 ttgtttgttt tgagcagcat aaatcccatt aaacatgaca ttgagatttc cagaggttgg
     6721 atttttaaaa ccagtagaaa atcctgcccc acttgccaca aagcggtgtt gctggtcttc
     6781 aactgaacgg gcaccaactg ccatgtaaga aatcaaatca tctacaagcg gaaggttttc
     6841 aggataaagc atttcatcag cagttgtcat ccctgtttct gtgatgacac gatagtgaag
     6901 atggcgaacg gctttgattc cattgataag actaggcgct tctgtcgcgt taggctggtg
     6961 aatcaagccc ttatagccat ctccgttggt acggggtttg gcagtataaa cacgcataac
     7021 cataaagata cgatctgcca cttcttcttg taggactgcc aaacgcttag cgtattcaag
     7081 gacagcttct tcgttgtcag atgagcatgg cccgattacc aagagaattc gctggtcttc
     7141 tccacgtata atggcttcta gctcttgatc tcgctgtgat ttcctctcca aagcctgacc
     7201 ttctaatttt gacaaggcac gaacttcttc tatatttatt ttagagcttt ttgctgtaaa
     7261 taccataact catctcctta taaagcaaac ggatacccag taaaaattta cttttcacaa
     7321 gatatccgcc tctaaactat ctcattttat tttcttttac cgtgacagtt tttaaatttc
     7381 ttaccagaac cacatgggca aagttcattg cgtccaatct ggctcaaatc caaatcttct
     7441 ggcatacttg cttggtgagc agcgatattg cgagtcgctg ttgtactgat atgacgttct
     7501 gcctgtggtc tttcttgttc atgaatttgt gctttcatca tcaagcgtgt cacatcaaac
     7561 tcaatcgaac caatcatatc attaaacata cggaaacctt ctgcctgata ctcaacaaca
     7621 gggttgttct gagcatagcc acgaagtcca accgcgttac gcaattgatc aagggcatcg
     7681 atatgatctg tccacttgtt atccaccact cgtagaatca aaactttttg gaattcttta
     7741 actgcttctt catcgcgtag ttttgaaacc tgactatcgt aaaccttcaa ggcacgttgg
     7801 aaaagctctt ccttgatggc cttatcagac aagcctgaca agtcttccat cgtaatagaa
     7861 tcttcaggaa gcaagttgta cttagcaaag ttcaaaattg cctctagttt ttcatcttgt
     7921 ttggcacgcg catgaccatc aacgacacgt tcaatcgtgc gcttaatcat agactgaatt
     7981 tcaggtgcca agtcacgatc tgcagtgatg acatcgtaac gttgagcata gataatctca
     8041 cgttgttcac gcatgacatc atcgtattga aggacttgtt tacgggtatc gtagttattt
     8101 ccttcgacac gtttctgagc tgcttcaacc tgacgcgtca acatgcgaga ctcaatcgcc
     8161 tcttcagaca tgttcaagcg ttcaaagatt cccttcaagc gttcagaacc aaaacgtttc
     8221 atcaaatcat cttcaagaga taggtagaat tgtgactcac ctggatctcc ttgacgacct
     8281 gaacgtccac gaagctggtt atcgatacga cgactttcat gacgttctgt accaataaca
     8341 caaagtcctc caagttcacg aacaccttca ccaagcttga tgtcggtacc acgacccgcc
     8401 atgttggttg cgatggtaac ggcaccacgt tgaccagcat tcatgatgat ttgggcttct
     8461 ctatagtggt ttttggcatt caagacttcg tgaggaacac cagctgcgac caatttctta
     8521 gaaatgtagt cactagtttc aaccgctact gtaccaacca agacaggttg acccttttgg
     8581 taacgagcct taacgtcttc gacaaccgct ttaaacttag attcgatact tgcataaaga
     8641 aggtctgagt ggtcaatacg ttgaacagga cggtttgttg ggattggaat aacacgaatg
     8701 ttgtagattt cacgaaattc ttcttcctca gtcttacctg tacccgtcat accagacaat
     8761 tttttgtaca tacggaaaag gttttggtac gtgattgagg cagatgtctt ggtttcatcc
     8821 tggattggca caccttcttt ggcttcaata gcttggtgca atccatcaga ataacgacga
     8881 ccttccatgg tacgacctgt aaattggtcg acaatcaaga tttcttgctc ttcgctcacc
     8941 acatagtcaa tatcgagaag catgatgtag ttggcacgaa gggcgttatc gataaagtgg
     9001 gtcaaagcca cgttttcgat gtcatagagg ttttcaagtt tgaagtagct ttcagccctg
     9061 tcaatccctg aatcagacaa accaatagtc ttagactgca catcgatgat gtagtcatct
     9121 ttgttcaaag attttacata gtggtctgcc atgtgataca actgactggt ttcaaccgca
     9181 ttggcacctg atacaatcaa aggtgtacga gcctcgtcaa tcaagataga gtcaacctca
     9241 tcgaccaagg catagttaag cggacgttgt accatgtttt cagcgcgaac gaccatattg
     9301 tcacgaaggt agtcaaatcc gatttctgag ttagttgagt aagtaatatc acactcatag
     9361 gcttcttttt tctccattgg agatttggta gccaagttaa tccctactga caaaccaagc
     9421 caagagtaca attcacccat ctcagtcgcg tcacgttctg acaggtattc attaaccgta
     9481 actacgtgaa cccctttacc tgaaagggca ttgaggtata ccggcatggt cgcagtcaag
     9541 gttttccctt cccctgtacg catctctggc acgtcaccat ggtgaagaac aatccccccc
     9601 atgacctgaa ccttataagg gaagagacct aggacacgtt tggcaccttc acggacaacc
     9661 gcaaatgctt cgtaaagcaa tgaatccagt gattctccat tttgataacg ttccttaaat
     9721 tcaactgttt ttgcttttag ttggtcgtca gtcaaagcag ccatttggtc ttcgtatttg
     9781 aaaaccttgt cagccatctt ttccagacga cggatttctc ctttatcatt ttcgataatt
     9841 gtttttaaaa tattagccat gtttttcctt actttaaatt ccgaatattt tagaatgttc
     9901 ctttaatttt agcacaatta ctgattattt tcaaggaaga atccccattt tgaaaaggaa
     9961 aaagaggtta gttttcaagc taacctctcc gacttttatg actgtttaat tgccaaaaat
    10021 tggcaaaatg gcaaatgtga taaataagtt aatccaaaga gaaagacagc aaatcagtcc
    10081 aaaaacaaag agactgactt tcttggacag caaggccatc aaaatggaca gaattccaaa
    10141 aactagcaag actttctgta tgatgaagat gtcaatcctt acccccagaa tcgctctatc
    10201 ccaagggata aagaagaaag acagccagat cagcagaact aaaccaatat aaaccttgga
    10261 atagcgtgta ataaatgatt ttagatgtgc cataagccat ctcctaggat tgtatatcct
    10321 ttacatattg tatatgtcca ttttcgacaa ctaatacatc tgtacaaagg tctgaaatat
    10381 cttctgatat gtgcgacgtc aggataacca gaccattttc ttttttcagg taagataaaa
    10441 tgacctgttt ggttaacctc acactcttct catccaaagc attcataggt tcatcgagaa
    10501 agagtataga aggagaggaa ataaaggctt gaatcaaggc cattttttgc tttgttccca
    10561 aggataaatg ccggtattca acgtcttcaa attcctgtaa atcatagtat tgaatccaat
    10621 aggcaattct tttttccgta actttagatg aaaaatacct taacagttcc aaattttctc
    10681 tcagggataa atgagataaa aactcgactt tttcaattaa aattcctgca tcctgaatat
    10741 aatgattttt tatcccgtaa actttaccat cttgaagaac ttttcctttg tcaagcttaa
    10801 tataaccagc aagtaccttt aaaagaaccg tcttgccaga tccattatca cctttaagtc
    10861 cataaatttt tcctgattga aacaccaaat tcaactgatt taaaataggt cttttcccat
    10921 aaattttctg tccatttata atttcaagtt tcatcgttta tcctttctta aatttctctt
    10981 ccaagcgact aacaatcaac ataactaata agcttaagag tagtaaatac actgttacaa
    11041 tatgatccat catccattct tgtcccacaa atgctaaaaa atagagaaaa aaggaaaaac
    11101 cagcgcccgc actaatcagt gctatcagat aagacagtag taagtacaac atcattaact
    11161 gagcaaataa aaagaaaagt gcgactgttt tgaaatgaat tcctagcgga agtaaaccaa
    11221 ggaaccagac aagtaaatct tgagtaaaaa atttagaaat gacaaccaat cgtttccttg
    11281 tataatcgaa aaaggagctt gattgatagc gaatcaagtg atggtaatgg tctgccaaat
    11341 cactttcaag taaaatgaca gagagccatc ctaccatgaa aagcgaaagg tttaacaaga
    11401 gtttggggat ttctaaattc acttcagaat agtcattata ggaaaaaatt gaaatcaatt
    11461 ctctcttgaa aaagataaca gcatcctgct tttgaaccgc tgttgacaat agatggttta
    11521 tcaaaatcat catcagaact atacgaagta gtaaaacaat ctttctcttt agcaaaatat
    11581 tgttaaaatt cttaaacatt atcttcatat ttttcaagca ccatgtaagg gacaatataa
    11641 aggatataag gagtaaagag tttcatcaaa cttacatccc catagctagc ttgcatcaaa
    11701 ctcgtcatag gaaccatata gtaaggcaag gcactataga gcagcataga accaagccaa
    11761 agaaacatca aataggtgat tgccgaacga gtcacatttc ctaaataatc aactatttta
    11821 aaaaaataga ctgcattgat gaaaataccg attagtagga cacaagtaaa gaacaaatag
    11881 cttgttatct ctccatctag gagcctttgt aaactactcc catcagaaaa tagagaattc
    11941 catccaagag gagaaaaagt cccaaagaaa taggttacaa ttgcaattat cagcacagtc
    12001 actaaatata tcaaacaagg gatacttgca acttgcaaag tcaactttct ttttaaccct
    12061 tgataactca cttctcttcc aatactcaat cgtaaaactt tatttttcag ctcaatatat
    12121 tgaaaaccta gcaaaacaat gatgattgga ataataaatg attgataaaa acctagattg
    12181 aactggagaa tacgaaaatc taatgctgat tttaataagt catttttaat tgcttgtcca
    12241 tcgaatttag ctagatcggg cgcatgaata tcaaaaaaga aataagtcat caaaacatca
    12301 cgataaacca aagcaagaaa aagcaaagta agcaagacat aaacaaattt atgccttgcc
    12361 aaagttaaga gaggaatctt cataagcaac ctcctacaaa tagaaagtaa tataatactc
    12421 aatgaaaatc aaagagcaaa ctaggaaact agccgcaagt tgctcaaagc actgctttga
    12481 ggttgtagat aagactgacg aagtcagtta catatataat ccaaggcgac gttgacgtgg
    12541 tttgaagaga ttttcgaaga gtataaatca atgccttacg cctttagact tccctagttc
    12601 ccgtctcaag tcaagcattt ttttgaaaca gatatagtct aaccagtcac tagcaaaagc
    12661 gataagtagt aataatagcc aaatacccca taacttgata tctgtcaaat ttctcaagac
    12721 gatattgaaa aacagaactg aaaaaactgt ccaagcaagg ctaaaaagag aatagagggt
    12781 gatgtaaatc cagtaaaaat agtaaaaaat tgggaaaaat ttactttctc tattatcctt
    12841 gtcaatccag ctgaaaaagt aaaaacaggg taataagatt atcattttaa acaagtgttc
    12901 catcacaagc ctcctttttt aaattgtttt ctttagtttc gttctattaa atattattat
    12961 caatataaac cttggaatag cgtgtaataa atgattttag aggtaccata agccacctcc
    13021 tacaaataga aaccgatata aatcaatgcc ttccaccctt agacttccct agttcctgtc
    13081 tcaagcgaaa catttctttg aaacaggaat aagttaacca attcatacca atagctagca
    13141 gaataaaaag aaaccaaatg ccccataact tgatatctgt cacatttctc aagacggtat
    13201 tgaaaaacag aactgaaaca actgtccaag caaggctaaa aagagaatag aaggggatgt
    13261 aaaaccagta aaaataataa aaaattggaa aaaacttact atttctgttg gccttttcaa
    13321 tccagttatc aaaataaaag tacggtgcta aaagtaagaa tttaaacaaa tgttccatca
    13381 ccgacatcac cctccttctt ttgatagcgt tttctattat tttattatat caaaaaaatc
    13441 cggaactgtc attccagatt ctactttttt atttgcgttt tcttgcgatg agatgaatcg
    13501 gtgttccctc aaaaacaaag gccttgcgga tttgattttc caagaaacgc aggtaagaaa
    13561 agtgcatgag ttcttcttca ttgacaaaga tgacaaaggt tggtggtttg gttgccactt
    13621 gggtcgcata gaaaatcttg agacgttttc ctttgtctgt cggtgttggg ttgatggcaa
    13681 tggcatccat gatgacatcg ttcaagacag ctgatggaat acgtgtattt tgactttcgc
    13741 tgatttgctt aatcatctca ggaagtttgt ggagacgttg cttggttaaa gctgatacaa
    13801 agataatcgg tgcgtaaggc aggtattgga actgctcacg gatatcttct tcccagtttt
    13861 tcatagtgtg gttatctttt tcaagcgtat cccacttgtt gaccacgata atcatccctt
    13921 taccagcttc atgggcaaat cctgcgatac gcttgtcgta ctcacgaatg ccttcttccg
    13981 cattgatgac catcaagacc acatctgaac ggtcaatagc acgcatggca cgcataacag
    14041 agtatttctc agtattttca taaactttac cagacttacg cataccagcc gtatcaatca
    14101 tggtaaactc ttgaccatct gtatctgtaa agtgggtatc aatggcatca cgagttgttc
    14161 cagcaacagg actagcaata acacggtctt cgcccaagat agcattgatc aagcttgatt
    14221 ttccaacgtt tggacgacca atcaggctaa acttaatgac atctggattt tcttcttcat
    14281 attcatttgg aaggttttcc acaatcgcat ctagtacatc ccctgtaccg ataccatgga
    14341 cagatgagat aggcagtggt tcacccaaac cgagggcata gaaatcatag atatcatttc
    14401 gcatctcagg gttgtccacc ttgttaactg cgaggataac tggtttgtgg gtcttataaa
    14461 gcttacgggc tacgtattcg tctgcatcgg taattccttc cttaccggac acgacaaaga
    14521 cgataacatc tgcttcttcc atggcaattt ctgcctggtg cttgatttgt tccatgaaag
    14581 gagcatcgac atcatcaatt cctcctgtat caatcatgct aaaagaacga ttgagccact
    14641 cacccgttgc ataaatacgg tcacgtgtca ctccttcgac atcttctaca atggagattc
    14701 gctcaccagc gatccgatta aatagggttg atttcccaac attgggacgt cctacaatgg
    14761 caatagttgg tagggccata atttctcact ttctacaata atttcttctg ttcaagattt
    14821 tttctagttg agcttggttc agcttgacca aactgttctg ctaggcgctg actccagctt
    14881 gtggtcgcac gcgccccagc atagtcagcc tgaacacggt cataagcttg gattgcctca
    14941 gttgactgtt cttggtattc ttcctcaaag acaacattct ctagtggcag tctcggtttc
    15001 atatcatgat gttgatttgg cacacccagt gccatcccaa agacagaata ggtgtagtca
    15061 ggcaggttaa agagctctgc cacttcttca gacttgtatc gaaccaaacc gataatcaca
    15121 ccaccatagc ccaagctttc agctgccaac aaggcgtttt gtccagcaag agctgcatcg
    15181 accgaactaa tcaagagacc ttccacacct tggggttgga aggtgtcggt atgaagtcgg
    15241 gctccctttt ctgctcggtt caaatctccg acaaagagaa ggaaaacagc agactggcga
    15301 atggcttctt gaggtaccaa ttcatacaag gcatctttct tctcttgact tcgtaccaca
    15361 atcacagagt aggattggaa attcttccaa gatgatgcca tctgggctgc tgtcaaaatc
    15421 tcatttaagt ctacttgggg aatttcttgc tctttaaacc tgcgcactga agtatgagcc
    15481 ttcatcaatt taatggtttc tgtcatcgac ggtttactcc ttctaaacga gtctcctcag
    15541 ccaaataacg gatgcgttcc atgacccgtc tggcttccca ggtttcgtca tttccatgtt
    15601 tcactttcgc aaaatgcttc tccaaatctt caaagttgaa gttggatgtg aaaaaggtcg
    15661 gtaaattttc ctgcatccga tattggagaa tgacctgcag gatttcgtca cgcacccaaa
    15721 cggttgattg ctcggcgcca atatcatcta aaatcaggac ctcagacagc ttaatctcat
    15781 ccaccaaggt cttaacattg ccatcactga tagcattttt gacatcaatg acaaagctag
    15841 gatagtggag gagagttgat gaaacaccac gtttttctga taaatcatga gctaaggccg
    15901 ccaccatgaa acttttaccc acaccaaagt ctccatataa gtaaagacct tttcgaatag
    15961 ctggatattg ctccacgaag gctagtagct tttcaaaaac tggtaagcgc cccaaatcat
    16021 ccaagtcaac ttgagccaaa ctagctttct tgagactggc tggtagattg attaacttga
    16081 gacggttctt aatagccgct tctttttcag ccgcgattag ctcaggagtt tcttcatatg
    16141 aaacatctgc atagccatga ttcttaacca aaatcggctt gtagcctttg gcaatataat
    16201 ccgtatcccc acggagaaac ttgtcacgct cggtgatgta ctgattaaac ttggagatac
    16261 tgcgatttaa ttcctctgga gtcaaggatt cttgctggat aaaagccgca acatcagggt
    16321 ccttcatgat tttctggacc aaatcttgat aataaaaacg gctgggttga cgtttgagta
    16381 cgtctccgac actttccatc taatctcctc ctttttctaa tcgagctaat agttcttgct
    16441 tcttacgttc tagttccaga cgagtttcct cgctggtttc attcttatat tcaggattac
    16501 tccatttagg aacattggtt tttactgggg cagtctgatt ctgtttttgt gtttttgctt
    16561 tctgtcctcg ctcacggatg cgcaagactg ccttttctgc cgaatgaatc ttttgatagg
    16621 catagtcatt ggctaccttc atggcatatt tctcattgat atttgccgaa tcgaccttat
    16681 taaaggtcaa taaaagaatg atattgatga cttcgtccag caagcccaag ccagccatct
    16741 gttgcaagag ttctctttct gtttgggtaa tggttccctt gcgtgtttgc ttgatttctg
    16801 ccaagaactg cagggcagtt ttacttttgg cttctttgat aatggttgtt tccttaagac
    16861 taaagtcaga ggaaactggt ttttgagcaa ttttttcacg catgcgtttg gttgaaataa
    16921 cctgagaaac agctgttgac ttggccaatt gataggtttc aaaccaagtc catttcttct
    16981 cctcggcaat agcaaagagg tttaagacat cggactgctc atccgcaaaa cgaagtccat
    17041 ctcgagccat cagccggcga aaatgttcca agtcaaaatc attggccact ttcttcttga
    17101 gaccaaggtc ttcttgactg cctagttctg ccaattctgg aaagacttga ttgagtgaga
    17161 caggtatttc ttcaccatca gcactttcaa ctttcaaatc ctccacagca gagtcgccaa
    17221 tctttttctc taagagtctg cgataaacag gatgctccaa gaagtcttga ctagatagag
    17281 gagcatggag ggctagctga taaacatccc ccttttgata gagggtcaag agattaaaag
    17341 cagataagat tttcaatgat tttatcagtc tatccatccc aaagttgaga tgattgagga
    17401 tacttgaaaa aagatattcc tttctaccat tatcccaaaa actgattgta taaagataaa
    17461 ggctcagtgc ctcctgaccg ataatcggga ggtagcactg taccagagat gaggtatctt
    17521 gcgacacccg attattcttt agataagaaa aacggtcaat tggcttcatt tatctttcct
    17581 ttttcttttt agaggactgg gtgatttgtt ggagcaagct ctctaactca ctgacatcct
    17641 taaaactacg atagacacta gcaaaacgta cataggtaat ctcgtccaat tcagccaact
    17701 cctccatgac gagtgaacca atgtcctcac tttgaatttc attttcattt cgaccacgga
    17761 gtttctgttc gatacgattg actaccatgt tgatttcatc acttgacaca ggacgtttct
    17821 gggctgagcg gataatccca ttaaagattt tatctctgga gaattgttcc cgtgtgccat
    17881 cttttttaac aaccactaag gttctttctt ctactcgttc gtaggttgta aaacggtgtt
    17941 ggcactcgtc gcactctcgt cttctacgaa tggtgttccc ttcttctgct tggcgactat
    18001 cgataacact tgacttggta gccccacatt ttggacaacg catcctttcc ctccttatcg
    18061 ttttcttttc attataccat tttttaagcg attcccaaaa caattcttct ttttgcttga
    18121 caagtttttt gttttgttgt attatttaat taagacaaaa gaataagaga aaggagacta
    18181 agatgtcctg gacatttgac aacaaaaaac ccatctattt acagattatg gagaaaatca
    18241 agcttcagat tgtttcccat acactggaac ccaatcaaca acttccaacc gtgagggagc
    18301 tagctagcga ggctggtgtc aatcccaata ccatccaaag agccttatca gaccttgaac
    18361 gagaaggatt tgtctacagc aagcgaacaa ctggacgatt tgtgactaag gataaggagc
    18421 taatcgctca gtcacgcaaa caattatcag aagaagaatt ggaacacttc gtttcctcca
    18481 tgacccattt tggctatgaa aaagaagaac taccaggcgt agtcagtgat tatattaaag
    18541 gagtttaagc ctatgtcatt actagcattt gaaaatgtat ccaaatcata tggagcaaca
    18601 ccagcccttg aaaatgtttc tcttgacatt ccagctggaa aaattgtcgg ccttcttggg
    18661 ccaaacggct caggaaaaac aaccctgatt aaactaatta atggcctctt acaaccagat
    18721 caaggacgtg tcctcatcaa cgacatggac ccaagcccag caaccaaggc cgttgtagct
    18781 tatttgcctg atacgaccta tctcaatgag caaatgaagg tcaaagaagc cctaacctac
    18841 ttcaagacct tctataaaga tttcaatctt gaacgcgctc atcatctact tgcagacctg
    18901 ggcattgatg aaaatagtcg tctcaagaaa ctatcaaaag gaaacaaaga aaaggttcaa
    18961 ctgattttgg ttatgagccg tgatgctcgt ctctatgttt tggatgaacc cattggtggg
    19021 gtggatccag cagcccgtgc ttatatcctc aataccatta tcaacaacta ctcaccaact
    19081 tctaccgttt tgatttctac ccacttgatt tctgatatcg agccaatctt ggatgaaatt
    19141 gtcttcctaa aagacggaaa agtcgtccgt caaggaaatg tagatgatat tcgctacgag
    19201 tcaggtgaat ccattgacca actcttccgt caggaattta aggcctaagc aaaggagatt
    19261 atttatgttt tggaatttag ttcactacga atttaaaaat gttaacaagt ggtatttagc
    19321 cctctacgca gccgtgctag tcctttctgc cctcatcgga atacagacac aaggctttaa
    19381 aaatctacct taccaagaaa gtcaggctac tatgctactt tttctagcta cagtctttgg
    19441 tggcttgatg cttacacttg cgatttcaac cattttcttg attattaaac gcttcaaagg
    19501 tagtgtctac gaccgacaag gctatctgac tttgaccttg ccagtttctg aacaccatat
    19561 catcacagcc aaactaatcg gtgcctttat ctggtcattg attagcaccg ctgtattggc
    19621 tctaagtgct gttattattc tggctttaac agctccagaa tggattcctc tttcttatgt
    19681 gattacattt gtagaaacac atctccctca gatctttctt acaggtatat ccttcctact
    19741 aaataccatt tcaggaatcc tctgcatcta cctggctatt tccattggac agcttttcaa
    19801 tgaataccgt acagcactcg ctgttgcagc ctacattggt atccaaatcg tcattggatt
    19861 tattgaactt ttcttcaatc ttagttctaa tttctatgtc aattcactgg taggactcaa
    19921 tgaccatttc tatatgggag caggtatagc cattgttaaa gaactcatat tcatagctat
    19981 cttttatctc ggaacctact acatcttgag aaataaggtt aatttgcttt aaataatttt
    20041 tacctagata tgtaacatac tcatagaaca aaagagacca ggcaaaaagt ctttaaaatt
    20101 agaaaacgca tagtatcaag tgttaaaaat ccttgatatt atgcgttttt tgtgagttga
    20161 cttatttcct ttcactatat cgcaaaatga aataagaacg gaacgatggg attttggaat
    20221 tcaaatcaat ttgtaagaat gttttagaag taatattatc ctattccaga ttcagttcac
    20281 tatacaattg agttttcaag caacttgttt acataatgtg tacataatta ggttcgtgat
    20341 tccacccttt tcacctttaa aaacctcgct ttcgcaaggc tcttctattt ataagataag
    20401 gcacgtttaa aggttttcca aatccctaaa tcatccgttt gaagaacgag actagcatac
    20461 atgcgtccga taaatcctgt tgctaccacc gcaaaaatca ctgtaatagc aagtgaaatc
    20521 catgcttccg ctccccccgc atagccatta atcgttcgaa acggcataaa gaaggtcgaa
    20581 ataaagggaa tataagaacc aatcttcaag aggagattgt caccagctgc acctagagct
    20641 gtcactccaa aaaaaccacc cataatcaaa atcatcaaag gcgacaaggc tttccctgag
    20701 tcctcaggac gagaaaccat agatcctagg aaggctgcca agactacata catgaaaaga
    20761 ctgatcaaaa taaagagcag ggtattcagt gagatagcat ctcccaagtg atccaaaata
    20821 ccagactgag ccaagaatgg caaatcttta aagagcaaaa cggcagccag accacctaca
    20881 acatagatcc caatatgcgt taaaatcact agaaacagag ccatcatccg cgcatagaaa
    20941 tagtgacttg cccttatgct agaaaaaacg acttccataa ttttggtgcc tttttcactg
    21001 gcaacttcct gagctgttac acccgcatag gtaatcagaa tcatataaag aaagaatcct
    21061 aaggcacctg ctgcaattgt ttgaataaac tttttatttt ccttggcttc atcaatcttt
    21121 tctgtgaatt gaattgtctg cgctaagcgt ttttcctgct cttgagacaa ggaagcagtt
    21181 gaacgattaa gctgattttg cagttcattg agtgtacctg taacctcaaa tttaattcca
    21241 ttttcaagcg atgtttcgcc atgataaact gcctttagaa cactatcttc ttgatcaatg
    21301 gtcaaataac cttttaattt ttcttcttta attgcttctt tggcacttgc ttcgtcttta
    21361 tagtcgaagt taacaccatt tacattcttc agtccttctg ctacagatgg cactgttgtc
    21421 actactgcca ctttattatt tttagccata gaagaacctt ggagatgccc aattcctaca
    21481 gagattccta aaaagaggaa cggcgaaatc accataaaga agaaactcca tgactcgaca
    21541 tgtcgaagat aggtttcctt gattacaacc cacatatttc tcatacttcc actcctgatt
    21601 ctagtttaaa gatttcatcg atagttggcg cttgttggtc aaatgttgcg atatattgac
    21661 cttgagtcaa gattgggaag agttcccttc cagcgctctc atcctccaaa atcaatttcc
    21721 aactgccttg tttggtcaag ctcacctgtt tgacatgagg aagattttcc aattcttcct
    21781 tgcttcgttc acttgaaaca aagagacgcg ttttcccgta ttgattgcgg acatcctgaa
    21841 ctggtccgtg caagaccaca cggccatctc ggatcatcag aatatcgtca caaagttcct
    21901 caacattggt catgacatgg tcagaaaaga taatggttgc tccgcgctct ttttcctgaa
    21961 aaatgacttg tttgagcaat tctgtattaa ctgggtccaa tccactaaaa ggctcatcca
    22021 agataatcag gtctggttca tgaatcagag taataatgag ctgaatcttc tgctgatttc
    22081 cttttgacag actcttgatt ttatctgtca gctttccttt cacttccaac ctcttcatcc
    22141 attgagggag tttttctttg acttctttgg catccatgcc ttttagagtc gccaagtagc
    22201 gaacttgttc aagaactgtc aatttaggca tgagactgcg ttcttcaggc agataaccaa
    22261 tccgagcata ggtctcctga cgaatatcct gaccatccag accgatttct ccctgatatt
    22321 ctaggaattt caaaatacta tggaaaatcg ttgtttttcc agcaccattt tttccgacta
    22381 gtcccaaaat acgacctggt cgcgcttgaa agtcaatacc aaacaaaact tgcttggatc
    22441 caaaactttt ctctagactt cttacttcta gcatctttca cctccgaaat ttctttcact
    22501 cattatactc ctttttgata gcctttacaa tgatttctgt ccatttttag aagactattg
    22561 ctgtgtaaaa tatagcctgg agcactttta tactcaatga aaagcaaaga gcaaactagg
    22621 aagctagtcg taggctgctc aaagtacagc tttgaggttg cagata
//
LOCUS       DAVAAL010000021        21836 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVAAL010000021 DAVAAL010000000
VERSION     DAVAAL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21836
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.021"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(45..893)
                     /locus_tag="VQM60_000663"
     CDS             complement(45..893)
                     /locus_tag="VQM60_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET1080553.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQSASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1045..1323
                     /locus_tag="VQM60_000664"
     CDS             1045..1323
                     /locus_tag="VQM60_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080554.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1325..3388
                     /locus_tag="VQM60_000665"
     CDS             1325..3388
                     /locus_tag="VQM60_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET1080555.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIKLMKKSEENKKTKQRYFIKMADKVVKYNFFGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(3466..4722)
                     /locus_tag="VQM60_000666"
     CDS             complement(3466..4722)
                     /locus_tag="VQM60_000666"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET1080556.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            4864..7329
                     /locus_tag="VQM60_000667"
     CDS             4864..7329
                     /locus_tag="VQM60_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET1080557.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEVTQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7394..8305
                     /locus_tag="VQM60_000668"
     CDS             7394..8305
                     /locus_tag="VQM60_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET1080558.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            8535..9233
                     /gene="dapD"
                     /locus_tag="VQM60_000669"
     CDS             8535..9233
                     /gene="dapD"
                     /locus_tag="VQM60_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET1080559.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVKI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9301..10431
                     /locus_tag="VQM60_000670"
     CDS             9301..10431
                     /locus_tag="VQM60_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET1080560.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGIAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            10443..10982
                     /locus_tag="VQM60_000671"
     CDS             10443..10982
                     /locus_tag="VQM60_000671"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET1080561.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            10966..11643
                     /locus_tag="VQM60_000672"
     CDS             10966..11643
                     /locus_tag="VQM60_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET1080562.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(11715..12614)
                     /gene="galU"
                     /locus_tag="VQM60_000673"
     CDS             complement(11715..12614)
                     /gene="galU"
                     /locus_tag="VQM60_000673"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET1080563.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(12636..13652)
                     /locus_tag="VQM60_000674"
     CDS             complement(12636..13652)
                     /locus_tag="VQM60_000674"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET1080564.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            13864..14244
                     /locus_tag="VQM60_000675"
     CDS             13864..14244
                     /locus_tag="VQM60_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET1080565.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(14418..14843)
                     /locus_tag="VQM60_000676"
     CDS             complement(14418..14843)
                     /locus_tag="VQM60_000676"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET1080566.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(14911..15561)
                     /gene="phoU"
                     /locus_tag="VQM60_000677"
     CDS             complement(14911..15561)
                     /gene="phoU"
                     /locus_tag="VQM60_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET1080567.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(15576..16328)
                     /gene="pstB"
                     /locus_tag="VQM60_000678"
     CDS             complement(15576..16328)
                     /gene="pstB"
                     /locus_tag="VQM60_000678"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET1080568.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(16330..17145)
                     /gene="pstA"
                     /locus_tag="VQM60_000679"
     CDS             complement(16330..17145)
                     /gene="pstA"
                     /locus_tag="VQM60_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET1080569.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17138..18001)
                     /gene="pstC"
                     /locus_tag="VQM60_000680"
     CDS             complement(17138..18001)
                     /gene="pstC"
                     /locus_tag="VQM60_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET1080570.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18119..18994)
                     /locus_tag="VQM60_000681"
     CDS             complement(18119..18994)
                     /locus_tag="VQM60_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET1080571.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19161..20492)
                     /locus_tag="VQM60_000682"
     CDS             complement(19161..20492)
                     /locus_tag="VQM60_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET1080572.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(20493..21200)
                     /locus_tag="VQM60_000683"
     CDS             complement(20493..21200)
                     /locus_tag="VQM60_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1080573.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21284..21769)
                     /locus_tag="VQM60_000684"
     CDS             complement(21284..21769)
                     /locus_tag="VQM60_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET1080574.1"
                     /translation="MAPTDKEDLIRTLELAPHIEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            21755..21836
                     /locus_tag="VQM60_000685"
                     /pseudo
     CDS             21755..21836
                     /locus_tag="VQM60_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         6325 a         4190 c         4745 g         6576 t
ORIGIN      
        1 aaccatacgt acggcaaggc gacgttgacg tggtttgaag agtattagaa tgctttccca
       61 accaaaatct ctgcttcgat ggtaatctct gtcagttggc tccagtcaat cttgctctgg
      121 tcaacgtgaa agaggagagg agtcatactg agtagggctt ggagttgttc tgctgtgata
      181 gtcttagtca gagaggcaga ttgactagat aggatggtaa agtgttcctg gaaatgctcc
      241 ttgatatctt ggttagaata ctccttgttt gtcagctggt cctgtactct ttgacggatt
      301 tctttgaggt gattttcagt tgggataacc tttatcaaga taccatcttt ggataaaacg
      361 cgacgaaatt ctccatagtt ggcaggtgag aagatatcaa gcaggatatc catgttagcg
      421 tcttttatag gaaggcgtgc caagtcgcca acgaaccaat tgactgccca gttgggttca
      481 cttttagcag cgatttggac tgaatctttg gagatgtcaa aggcatagaa agttttttca
      541 gagtgacttt cttgtagttt gcgagaatag aatccttcac cacaaccgat atccaaaatt
      601 gttgtggtag tttttgagct tgcaagcaag tcagatacag catctaagat agcttggtaa
      661 aagccggctt ctaggatttg ttgacggttt tgaaaatttt ccttgtcata gttagcagat
      721 tgcttgattt gaggtgctag attgacatag ccaaatttcg ccaagtcaaa agaatgacgg
      781 ttgcagcact tgaaattagt ctctaacaga gtcagatttt cttgacagat aggacaggca
      841 aaggcagtcg cagaagcaaa acgctgaagt ttgggcttga gatttgtatt catagtttaa
      901 gtgtatcaaa agaggcaaat gaaagcaact ttaggaataa gtagatggga gattcagtag
      961 aaataaaact aattctccaa tttatagaat gaaattgctt ttatatctaa attgctatat
     1021 aataatgata aggaggaaat aagtatgtta aaagaagtat taaccgttgc aaaagttgcg
     1081 aaaaaatctt cattattttt gggtggtgtc gcatttggta cccttggttt gaaaatctta
     1141 gcaagtaagg aagctaaaaa aggttattct aaagctttgg ctaaggctta caagttgaaa
     1201 gacgagctag atgcatctgt ttctgttgtg aagcaacatg gagacgatgt cttgcaagat
     1261 gccaaatatt tgtacgagca agagaaaaaa gaagagcaat tagatagcct tataggtgaa
     1321 taatatgtct tttaaagtgc tacatagagg ataccaacat atccgactat catcttcttt
     1381 ttcacttacc ttggatattc aagactatct tcgttccttg gcgagagatg aaaaggggat
     1441 tgagtctatc cagttttaca tggatcaaca gcactttact ctacgcataa aagaaggctt
     1501 ttctgtatta gataatgcag aagccttttt aaaaagaatt gataaaggga aagtttctga
     1561 gttgatgact cttcccattc gtagagaaga gagtgcttat tctattgttt caggtgcagc
     1621 ggttaagcgt gtactttttc gtagttttgt gccgtatcct attcgctata tatggacttg
     1681 ttatcaggct ttgggttata ttagagaagc ctatcaaaca ctagcgcgta aggaactaac
     1741 gatggaagtc ttggactgtt cggctatttt attgtctttg tttatgaacc aatccaagac
     1801 agctagcaat atcatgttta tgcttgattt ggggaatcat ttagatcagt ggtccttgaa
     1861 aaaaactgca acagatttag aacagagtct tcttgcaaaa gagagcgatg tattcctagt
     1921 acagggcgat acggttgtta gtatcaagag ttccgatgtt caaataggag atgtcttgat
     1981 cttatctcaa ggaaatgaaa ttctgtttga tggacaagta gtttcaggtt taggtatggt
     2041 caacgaaagt tccttgacag gagagagttt tccagttgaa aaaagagagt ctgatttggt
     2101 ttgtgcaaat acagtattag aaactggaga gttacgcatt cgtgtaacag ataatcagat
     2161 gaacagccgt attttacagc tgattaagtt gatgaagaaa tctgaagaaa acaagaaaac
     2221 gaaacaacgc tatttcatca agatggcgga taaagtcgtc aaatataatt tctttggggc
     2281 tgggctgact tacctattga caggttcttt ttctaaagct atttctttcc tattggtcga
     2341 tttctcctgc gctttgaaaa tctctactcc tgtagcttat ttgacagtta tcaaggaagg
     2401 gttgaaccgt gaaatggtga ttaaggatgg agatgttctg gagaaatatc tggaagttga
     2461 tactttcttg tttgataaga caggaacaat cacaactagt tatcctatag ttgaaaaggt
     2521 gttacctttt ggagactata gtgaggaaga tattctcaga atcagtgcct gtcttgagga
     2581 acacatttat catcctattg ctaatgccat cgtcaagcaa gctgagatag agggaattga
     2641 acatgaggaa atgcatggga aactccaata tatcgcaagc aaggggatca aatctcatat
     2701 agatggccaa ccagttctta ttgggaatta tgtcttgatg caggatgagc agattcatat
     2761 cagttcagaa caaaatgctt taattgaaga gtacaagagt cactacaatc tcttattctt
     2821 ggcttatcaa aatgaattga ttggaatgtt ctgcattcat actcctttga gaaaagaagc
     2881 aaaaacagcc ttggataaac ttaaggcaca ggggaaaaaa ttgattctgg caacaggaga
     2941 caccttgatt aggacagagg aattagtcaa agatttgccc tttgatcagg tctatacaga
     3001 cttgaaacct gatgggaaat ttgagttagt agagaaactg cagaaagcag gtcacactat
     3061 tttgatggtt ggagatggat tgaatgactc agcggcttta actctatcag atatcggtgt
     3121 ggtgatgaat gagagtgcag atatttctaa gcagatgagt gatatcttat tgttagataa
     3181 tcgtttggat ttcttccaag agttggattc gttatcatca tctttgcaaa cactcatcaa
     3241 gaaaaatatt caagataccg ttgtcgtaaa tagtagtttg attggcttcg gcttatttaa
     3301 ttggctcagt ccttcaaatc tctctatctt acataatcta acaaccttgc gcattgtcct
     3361 gcgtagcctg tctattaaaa atagatagat gagagttatt aacagcaaaa aggagttacc
     3421 tttctcgagg ttaactcctt tgtttataga taaatgttga atagtttagt aagtcaatac
     3481 aaagtatttt ttcttcccac gacggataac agtcagttca ttctctaact tatcagcgtc
     3541 actcaagaca tagtcaaggt cttggatgcg gtcgccgttt acgtagatgg ctccgttttg
     3601 gacgtcttca cgggcttggc gttttgagtt aactatacca gatgagacga gcagttccac
     3661 gatattgttg tgttcgtctg cctgtacttg gtagtttggc acaccacgaa gtccttgttt
     3721 gagttctttg acagaaaggt ttttgatgtt tcctgcaaag agttgctcag tgatgttaag
     3781 tgcttctttg taggcttctt ctccgtgaac aagtgtaaca acttcacgag ccaagacttt
     3841 ttgagccaag cgttcgtgtg gcgctgcttc aaattgttta cgaatatctt caatctcatc
     3901 aagtgacaag aaagtaaaga ttttcaagaa gcgaacagcg tcagcgtcca tcacgttcat
     3961 ccagaattgg tacatttcgt atggagaagt cttttcggga ttgagccaga cggcatttcc
     4021 ttctgattta ccaaatttct taccagttgc atctgtgatt agtggaacag tgataacgtg
     4081 accagtcttg tccgccttac gacgaagcaa ttcggtacca gctgtcatat ttccccactg
     4141 gtcagaacca ccgatttgaa gagtgacatt atggtcttgg ttaaggacga agaagtcgta
     4201 cccttgcatg atttggtaag cgaactcagt gtaagaaatt cctgtttcga tccgtttttt
     4261 aacagattcc ttactcatca tgtagttgac cgtgaagtat tttccaatat cacggaggaa
     4321 gtcaatgaag ctgatgctgc caaaccagtc gtagttgttg accatgacag ccttgttttc
     4381 gccattttca aagtcaagaa aacgagaaag ttgtccttgg atagacttga cccagccatc
     4441 tactgtgtct tttgtttgga gactacgttc agcatctttg aaggacggat ctccgatgag
     4501 acctgtagca ccgccaacga gcgcataagg tttgtgacct gctagttgca agcgacgact
     4561 tgtcaagatt gcgacaaggt ggcctaggtg aaggctgtca gcagttggat cgtagccagt
     4621 ataataagaa acttgacctt cttctagggc tttacgcaaa gcttcttcat cagtcgtttg
     4681 aaatatcaaa ccacgctctt ttagctcatc aaaaatgtgc atgtgtcttt tctccttttt
     4741 aaaattattg tttctaccta ttgtatcaca aacttgggca atagcctagc agaataggga
     4801 agaaagtggc tattttattg aaaatttccc ttttgtggta taatagataa agtgaggaaa
     4861 tccatgcaaa atcaattaaa tgaattaaaa cgaaaaatgc tggaattttt ccagcaaaaa
     4921 caaaaaaata aaaaatcagc tagacctggc aagaaaggtt caagtaccaa aaaatctaaa
     4981 accttagata agtcagccat tttcccagct attttactga gtataaaagc cttatttaat
     5041 ttactctttg tactcggttt tctaggagga atgttgggag ttgggattgc tttggggtac
     5101 ggagtggcct tatttgacaa ggttcgggtg cctcagacag aagaattggt gaatcaggtc
     5161 aaggacatct cttctatttc agagattacc tattcggacg ggacggtgat tgcttccata
     5221 gagagtgatt tgttgcgcac ttctatctca tctgagcaaa tttcggaaaa tctgaagaag
     5281 gctatcattg cgacagaaga tgaacacttt aaagaacata agggtgtagt acccaaggcg
     5341 gtgattcgtg cgaccttggg gaaatttgta ggtttgggtt cctctagtgg gggttcaacc
     5401 ttgacccagc aactaattaa acagcaggtt gttggggatg cgccgacctt ggctcgtaag
     5461 gcggcagaga ttgtggatgc tcttgccttg gaacgcgcca tgaataaaga tgagatttta
     5521 acgacctatc tcaatgtggc tccctttggc cgaaataata agggacagaa tattgcaggg
     5581 gctcggcaag cagctgaggg aattttcggt gtagatgcca gtcagttgac tgttcctcaa
     5641 gcagcatttt tagcaggact tccacagagt cccattactt actctcctta tgaaaatact
     5701 ggggagttga agagtgatga agacctagaa attggcttaa gacgggctaa ggcagttctt
     5761 tacagtatgt atcgtacagg tgcattaagc aaagacgagt attctcagta caaggattat
     5821 gaccttaaac aggacttttt accatcgggc acggttacag gaatttcacg agactattta
     5881 tactttacaa ctttggcaga agctcaagaa cgtatgtatg actatctagc tcagagagac
     5941 aatgtctccg ctaaggagtt gaaaaatgag gtaactcaga agttttatcg agatttggca
     6001 gccaaggaaa ttgaaaatgg tggttataag attactacta ccatagatca gaaaattcat
     6061 tctgccatgc aaagtgcggt tgctgattat ggctatcttt tagacgatgg aacaggtcgt
     6121 gtagaagtag ggaatgtctt gatggacaac caaacaggtg ctattctagg ctttgtaggt
     6181 ggtcgtaatt atcaagaaaa tcaaaataat catgcctttg ataccaaacg ttcgccagct
     6241 tctactacca agcccttgct ggcctacggt attgctattg accagggctt gatgggaagt
     6301 gaaacgattc tatctaacta tccaacaaac tttgctaatg gcaatccgat tatgtatgct
     6361 aatagcaagg gaacaggaat gatgaccttg ggagaagctc tgaactattc atggaatatc
     6421 cctgcttact ggacctatcg tatgctccgt gaaaagggtg ttgatgtcaa gggttatatg
     6481 gaaaagatgg gttacgagat tcctgagtac ggtattgaga gcttgccaat gggtggtggt
     6541 attgaagtca cagttgccca gcataccaat ggctatcaga ccttagctaa taatggagtt
     6601 tatcatcaga agcatgtgat ttcaaagatt gaagcagcag atggtagagt ggtgtatgag
     6661 tatcaggata aaccggttca agtctattca aaagctactg cgacgattat gcagggattg
     6721 ctacgagaag ttctatcctc tcgtgtgaca acaaccttca agtctaacct gacttcttta
     6781 aatcctactc tggctaatgc agattggatt gggaagactg gtacaaccaa ccaagacgaa
     6841 aatatgtggc tcatgctttc gacacctaga ttaaccctag gtggctggat tgggcatgat
     6901 gataatcatt cattgtcacg tagagcaggt tattctaata actctaatta catggctcat
     6961 ctggtaaatg cgattcagca agcttcccca agcatttggg ggaacgagcg ctttgcttta
     7021 gatcctagtg tagtgaaatc ggaagtcttg aaatcaacag gtcaaaaacc agggaaggtt
     7081 tctgttgaag gaaaagaagt agaggtcaca ggttcgactg ttaccagcta ttgggctaat
     7141 aagtcaggag cgccagcgac aagttatcgc tttgctattg gcggaagtga tgcggattat
     7201 cagaatgctt ggtctagtat tgtggggagt ctaccaactc catccagctc cagcagttca
     7261 agtagtagtt ctagcgatag cagtaactca agtactacac gaccttcttc ttcaagggcg
     7321 agacgataat tctctaaatg aagtggccaa tcaatggatt gccacttttt tcctttcgtg
     7381 ttacaataag ggtatgaatc agtatcagaa aaagattgtt aacggaaaaa tttattcgct
     7441 cctatccggc ttaatatggg gaatctgtgg aattttagga gagtacttct ttactcatta
     7501 tcaggtgtct tcgggctgga ttacctctat gcgtttgaca ctggcaggga gtcttgtact
     7561 catttggtct gcaatacaat taaaatcgca agtgctagat atttggcgag acaagaaaaa
     7621 ttacctgccc tttttagcct atgctatttt ggggattttt tcagttcagt attttttcta
     7681 tctctgtgta gaatactcaa atgctacgac agcaactatt ttacagttta ttagccctgt
     7741 ttttatcctc ttttacaatc gcttggttta tcaaaaacga gcgtcaaaaa gcgctgtttt
     7801 ctatgttttg gttgccatgc tgggtgtttg cttgatggcg acaaagggag atctctctca
     7861 gttatccatg acgccgctag ctcttataac aggtttgctg agtgccatgg gtgttatgtt
     7921 taatgttatc ttgccccaac cttttgctaa gcgttatggt tttgttccta cggttgggtg
     7981 ggggatgatt ttggcaggtt tgtttagcaa tgtcctctcg ccggtttatc agctttcctt
     8041 tactcttgat atttggagta tcttgatttg cctcattatc gctttctttg gaacggcttt
     8101 tgcttttttc atttccatga aggctgtgtc cttggtttct cctttggtgg ttgccgttat
     8161 cagtgccagt gaacctctct cttctgctct cttgagtgtt ttgttcttag gattagtagt
     8221 ggattggtcc ctccttctag ctatagcctt gattatttta cccatgatat ttttgtctat
     8281 agaagaagcg aaagaaagta gataaaaagt cttttcttaa ttctaaaagt gtgctatact
     8341 agaatagtca ataaaacacg gggagatagt tgtgaagagt gtttttaggt tgtatcggta
     8401 ggggcttgct tttaccgaca gcacgtttta tctcacatcc ctaaaaaatc atacctaaaa
     8461 acagacaaaa aggtttcaaa tttgaggcct tttttggtag aataggtatc attataacga
     8521 accaggaggc acctatgact gctacaaaaa tgaacgctca agaaattatc caatttatcg
     8581 ccaatgctga aaagaaaacc agtgtcaaag taacctttga ggggcaactc gcaactgctg
     8641 tgcctagctc tgttgtcaaa ctaggaaatg tcctattcgg agactggaag gacgtggctc
     8701 cgcttcttga aggtttggta gaaaatcaag attatgttgt cgagcaagat gctcgtaatt
     8761 ctgcagttcc tttgctagat aagcgtgcta tcaacgctcg tatcgagcca ggtgctatta
     8821 tccgtgacca ggtgaaaatt ggtgacaatg ctgttatcat gatgggagct gttatcaata
     8881 tcggtgctga aatcggtgct ggaaccatga ttgacatggg tgccatcctt ggtggccgtg
     8941 ctatcgttgg aaaaaatagc cacgttggtg caggtgcagt tttggcaggt gtgattgagc
     9001 cagctagtgc tgaaccagtc cgtgtcggag acaatgttct tatcggggct aatgcagtgg
     9061 ttatcgaagg agtccaaatc ggtagtggtt cagttgtcgc agcaggagct attgttaccc
     9121 aagatgtccc agaaaacgtg gtagtagcag gtgttccagc tcgtattatc aaagaaattg
     9181 atgcccaaac tcaacaaaaa acagcgttag aggatgcgct tcgtaccttg taattgtaaa
     9241 agtaaaaaag aggcggaacc ctttttccag cctctttctg ctatatcgga ggacagatag
     9301 atgttagatt tgattcagac tagacgagat ttacaccaga ttccagagat tggcttggag
     9361 gagttcaaga ctcaggctta tttgctggat gtgattgaga aattgactac gggcaaggat
     9421 tttgttcaaa ttcgtacttg gcggacaggg attttggtct acctgcaggg aagtcagccg
     9481 gagcgaacca ttggttggcg gacagatatt gatggcctgc ctatcgtcga acaaacaggt
     9541 ctaccttttg cttctcaaca ccaaggtcgc atgcatgcct gtggccatga ttttcatatg
     9601 accattgcct taggctgtct tgagcgcgcc cttgaggagc aacctaaaaa taatctgctc
     9661 tttctatttc agcctgctga agaaaatgaa gctggtggta tgctcatgta tgaggctggt
     9721 gcttttggag attggttgcc agatcaattt tatggcctcc atgtccgtcc agatttgaaa
     9781 gtcggccaga ttgcgactaa tactcataca ctctttgcag gaacttgcga ggtgaagatc
     9841 cgtttcaaag gtaaaggtgg ccacgcggct tttcctcatg aagccaatga cgccttggtg
     9901 gcggctagtt actttgtgac ccaagtgcag tcagttgtta gccgcaatgt aaacccaatc
     9961 gagggagcag tggtgacttt cggcgttttt caagctggaa ccaccaacaa tgtcatcaca
    10021 gacacagctt ttttacatgg aactattcgt gccttgacac aggacatgag tctcttggtg
    10081 caaaagaggg taaaaacagt tgcagaaggg attgcagctg cctttgatat ggaagtcgaa
    10141 gtggaactca aacaaggtgg atacctacct gttgagaaca atccagcctt ggcgcgtgaa
    10201 ctgatggact tctttgacga aaaagacgga atcgagttga ttgatatcga acctgctatg
    10261 acaggcgagg actttggtta tctcctttca aaggttgatg gcgtgatgtt ttggctaggt
    10321 atcgatagtc cctacgctct tcatcaccct caaatgagtc ctaaggaaga agtcttagct
    10381 attggggtgg ctgcggtgtc tagtttcctg aaaaagaagg cagcagaata gagggcttgt
    10441 ctatgaaatc ggaattacgc aagcaagtct tgcatgaaat gaaggcttta tctcaagaac
    10501 aaaaacaggc tatagaccaa gctttaaccg agcgaatttt acaacacccc ttttatcaag
    10561 aagccaaggt catcgcaacc tacctctctt tttctcatga gtttcaaacg cgggaactga
    10621 ttgagcaggc gctgaaggac ggcaagaagg ttttaatacc caaaacttat cccaaggggc
    10681 gcatggactt tgtagtctat gatccgcagc agttggtaaa aactgccttt gccttactgg
    10741 agccacaggg agatttggaa gtggtggatg tatctcagat tgatttgatt catgttcctg
    10801 gcctggcttt tacgacggat ggatatcgga ttggatatgg tggaggttat tatgaccgct
    10861 atctggaaca tttttctggt catactttga gtacggttca tccttgtcaa attcaggact
    10921 ttatacctga aaaccatgat attcctgttc aggaggtatt aattgatgaa ggaaatcttt
    10981 gatagacgtt accctgtgac gagtttcttc ctcttagtga cggccttggt atttttacta
    11041 atgttggtca ctgcaggcgg aaactttgac agggcagata cattatttcg atttggagcc
    11101 atgtatgggc cagctattcg cctctttccc gagcaggttt ggcgtctctt gtctgccatt
    11161 tttgttcata ttgggtggga acatttcatt gttaatatgc tttcacttta ttatcttgga
    11221 aggcaggtag aggagatttt cggctctaag cagttctttt ttctctatct cttatcagga
    11281 atgatgggca atctctttgt ttttgtattt agtcctaaat ccttagcagc aggcgcctct
    11341 acctctcttt atgggctatt tgccgcgatt attgttcttc gctatgcaac tcgcaatcct
    11401 tatatccaac agctagggca atcctatctg acactttttg tggttaacat tattggaagt
    11461 gttctgattc caggaatcag cctagcaggc catatcggtg gtgcagttgg tggcgcattt
    11521 ctagcagtta tctttccagt tagaggagaa aaacggatgt ataataccag ccagagatta
    11581 ggagcggtag tcttgttcgt aggactcgcc attttgcttt tctacaaggg aatgggaatg
    11641 tgacgaagtt gaaagaaaat ttgtgtaatg aaaaaagata aggcattttt tgaaccttat
    11701 ctttttattt tattttattc cttctcagtc aattcttttc caagttggat gaggtaattc
    11761 ttcaaatcat ctttgacttg tgggtgtttg agggcgtagt cgatggatgt tttcatgaag
    11821 ccaaacttgt ctccgacatc gtaacgagcc ccattgaact cacgagcaaa tacacgttgt
    11881 gttttattga gggtgtcgat tgcatctgtc agctgaattt catttcctgc acctggagct
    11941 tggttttcga ggatttggaa aatttcaggc gtgaggaggt agcgtccgat aatagcaagg
    12001 tcgctaggag cgtcctctgg agctggtttt tcaacaaagg tttcaacact gtaaagacca
    12061 tcttttcctt cgccttgcgg agcaataacc ccgtaagcag atacttcgtc atgagggact
    12121 ggcatgacag cgatagtaga cgcgtgggta cgctcgtagt catccatgag ttgtttggta
    12181 agtggaacag ccttttcgtc tgtgatatcc atcaagtcat caccaagcat aacgacaaaa
    12241 ggttcatttc cgacgaaagc cttggcttgc aaaacagcat ctccgagacc gcgtggatga
    12301 gtttggcgga taaaatgcag acgcatgtca gttgttttat caactagctt caaaagatct
    12361 gttttccctt tttctttgag gttatattcc aattcgaagt ttgaatcaaa gtggtcctca
    12421 atagaacgtt ttgatttacc agtgacaact agaatatctt caatacctga tttgagagcc
    12481 tcttccacga taaactggat agttggtttg tctacgattg gcaacatttc tttggcaagg
    12541 gccttggttg ctggtaaaaa tcgagttcct agtccagcag cagggatgac tgcctttcta
    12601 acttttgatg tcataaaaat cctttctata gagggttaag accactcatt ttctgcttta
    12661 aattcattgt tcatgatgtc ataaatggca tctttgatat tggttccgtg ataaataact
    12721 tggtaaatag cctgtgtaat gggcatatag actccaagtt cttgggctag ttcataggct
    12781 gctcgagtcg ttgaaattcc ttcgattacc atgcccatat tagcttctat atcagctagg
    12841 gattctcctc gtccgagagc atctccagct ctccagttac gagagtggat ggaagttccc
    12901 gttacgatca aatctcccac accagataag ccgctatagg tcaatggact ggccccgagt
    12961 gctaccccta ggcgggtgat ttctgctaaa cctcgagcga tgatggctgc cttagcatta
    13021 tcaccaaatc caagaccatg taaagctcca gcaccgacag caataatatt tttaagagca
    13081 ccagcagttt caaccccgat aacatccgta ttggtataaa gtcggaagta gtgattacta
    13141 aatagcttct gaacgtattg agctgtttgt aaatctttag aagcagcagt tattaaagtt
    13201 aggtcacgca caatggtctc ttctgcatga ctaggccctg aaacaacgac gatatcacta
    13261 cggagatgtt caggaatttc ttcttcaaga atggttgata atcgtttatg gctatcaggt
    13321 tctaatccct ttgatgcgtg catgatgata accttatggt ccaaggtttg tgcaacttgc
    13381 tgggcaacaa gtcgtgtcac ttttgttggg acaacaaaca aaatcgcatc cacatctttc
    13441 aatgtttctg ctaagtcggt gtaggcaatg atattttcgt ctagaacgac atctttaaag
    13501 tagtgcttat tagtatggtg tgtattaatt tcattgattt gctcgggaag atttccccaa
    13561 atacgtacct cgtgtccatt gtcatttaag acttgtgaaa gggcggttcc ccaagaacca
    13621 ggccccaaga cggcgacggt ttgtttttcc atgatttcct ccttgataga gttctttctc
    13681 ttattctatc atattctagg ggattttgca cttgcattac tggggtatgg tggaaaaata
    13741 cacaaaaacc gagatgaagg gataaactct tagtctttag ttaaagtgag aagaaatctg
    13801 atagatagtt tgattcagta tatgctataa atagaggtaa acaactcagg aggttcttgt
    13861 aggttgcgag agtttggcga aaaaattaaa agattacgtt tggctaaaaa aatcagtcgt
    13921 tcagaatttt gtggtgatga gtctgaatta agtatccgtc aattaattag aattgaaaat
    13981 ggagaatcca gaccaacact aacaaagtta aaatatatcg ctgaacgttt gggggttgaa
    14041 gattacaagt tgatgccaag ttatatagag ttggataagg aatacctaga attgaagtat
    14101 ttcttgatga ggactcctac atacgaagat gaaactatcg cccaaaagaa agagagtgtt
    14161 ttgataagat ttttgaagag tattatgata ggctacctga ggaagaaaga tttatcatcc
    14221 caaattattc atatctggca ctaacgaact acacagttca aaaattacca gaaaagctag
    14281 ttgaaatact gtccttctgg tgattttgct taatttctga tgttcgagac ataattgagg
    14341 agatttgtct tgattttttc ctaaaaaagg gcagactcct cccttggttt gatgctcgat
    14401 ttttaaaata aggtgactta ttctgttagg catgttttcc cctgccctct agctctatat
    14461 ggaacaggga gtatcagatt tatctggcag attgtatcaa ataaggcttg aaactgtttt
    14521 gaataggcgt atagacttga gaatttgaga atatgtcgtc tagcagtaga gacatatttt
    14581 ccggcaatat gaaggaagag acgtcgaaaa cgcttgatag tcaaggcctt tacttcatca
    14641 ccagctagct gctttaaaaa gaggtagaga ttataagcca gacagcccat catcatacgt
    14701 acttcattct taatcatggt cgaactatct gtcttatccc caaagaatcc tgctttcctt
    14761 tctttgataa agttttcagc attccctcgt tcacgataga attgaaacag ctcctcacta
    14821 tctacactgc ttaaattggt caaatgaact tttttacaac cataaaaaag agcacttaat
    14881 cgtactcttc tcttttaagg attagttgaa ttaattcaaa tccactagtt ctcctgtttc
    14941 taggtagact aggcgttcac aaatgttagc aatgtaatca gcgaagcgtt ccagatgccc
    15001 tatgatataa aggtattgag ttccattggg aattgaggtt tcttggtctt tcataagtcc
    15061 aatgatttcc tttgataagg cataataata ttggtcaatc tgttcatctt tttgagcaat
    15121 actaatagct tttgaggctt ggtgcaaagg aaaggcaacc aataaatcgg ctagcatgct
    15181 gagggataat ttacccattt ggtgtaactg ttcttcgtca ggggctagtt gattttcttt
    15241 tagttgcaaa acagctttgg caatgcctgc catatggtct cccatacgtt caaggtctga
    15301 acaagaagac atgatgctaa ttacaaatcg aaggtcagac acttgtggct gctgcaaggc
    15361 caacaaacgg gcacaggtca attcgatagc gctttgacct tggttgatag catgatcctt
    15421 attgataatt agctctgcca tctccttgtc tttggaggct aaggccagta aggcttttga
    15481 agctgtttca aggacaagtt gccctagtcc taaaaaggat tgttctaatt catgcaattc
    15541 taagtcaaat tgatttctca taggtttttc cttccttatc cgaaccgtcc tgaaatatag
    15601 tcttctgtgc gctgatcttt tggattggta aacacgtcaa cggtatctcc aaattcgcaa
    15661 atttctcctg ttaagaaaaa agcagtttta tctgaaatac gtgaagcttg ttgcatgtta
    15721 tgggtaacaa tgataatcgt ataatccttt tttagttgct gaatgaggtc ttcaattttt
    15781 aaagtggaga tagggtctaa ggctgaagtc ggctcatcca ttaacagaac atcaggttct
    15841 actgctaaag ctcgcgcaat gcaaaggcgt tgctgctgac cgccagataa ggacatggca
    15901 ctctttttaa gatcatcttt gacttcttcc caaatggctg ccccttttaa agatttctcc
    15961 actaaggcat ctaattgttt tttgtctcga ataccatgtg tccttggacc ataagccacg
    16021 ttatcataga tagacatggc aaagggatta ggctgttgaa aaaccatccc tacacgcttg
    16081 cgtagctgat taaggttgaa tttgctacta taaatatctt gctcatctaa gaggacttgg
    16141 ccttcaatat ggcaagaagg aaccaaatcg ttcatccgat taagggtttt tagaaaagtt
    16201 gatttgccac aaccagatgg gcctatcaag gcagtaatct gtctttctgg taattgaatc
    16261 gaaatatttt ttaaggcttg aaaatccccg taaaataaat ctaggtgtct aactgaaaat
    16321 gttcccatac taggaagctc ctttcacaag tttccgagat aataagcttg atagagtatt
    16381 tatcattaaa acagtaataa tcaaaatcac gccggtagca taggcttcat tgacatgtag
    16441 cccctcactt gacagcatat acatatgtag ggctagagaa cggcctgaag acatgagact
    16501 acttggcgta ttggtagagg tacctaatgt atacatgagg gcagctgttt caccaacgat
    16561 acggccaata gctagtatca ctccagctaa aatacctggc atggcaactg gtagaacaat
    16621 tctaaaaaca gtccgtaact tacctgcccc aagtccataa cttgcttgac gcatgctatc
    16681 actaacagat aaaagggctt cttctgttga gcgaataatg actggcaaca ccatgataac
    16741 tgaggttaag attcctgata acagagagta ttgaaaacct aagaagacta caaagaagag
    16801 catgccaaac agaccaaaaa caatggaagg aatcccagac aaggtatctg aggccaatcg
    16861 catgatttta acacaaaggg aatctttttt tgtatattcc acaagataaa aaccagcaaa
    16921 aatccctatg ggcaaggcta aaagaagagc accaaaaacc agaataacgg tggaaataat
    16981 cgctggcata agggaaatgt tctcagaagt ataagtccaa gaaaagaggg atagacttag
    17041 atgaggtaag cctttgatga ggataaaacc aatgattaaa aagagagagc caaaggttaa
    17101 agctgaaaaa caataaacga gaagttttag caggtattta ctcataagat gattttcctt
    17161 tcaagtaggc aaagtaggca ttaatcaaga gaataaggaa aaagagaact gctgaggttg
    17221 caataagggc ttccctatgc tgacctgatg cgtaagccat ttccagaaca atattggttg
    17281 ttaaggttct ggttcctgaa aagagtccac ttggaataat cggctggttg cctgccacca
    17341 aaataactgc catggtttca cctactgcgc gaccgattcc taaaataact gctgataaaa
    17401 taccagatct cgcagctggc aagatgacac taaaaatact ccgttcatga ctagctccta
    17461 gagccaagct accagaataa tacgttttgg gaactgttcg gatagcagat tctgacaaac
    17521 tgataatggt tggcaaaatc attattccta atagtaacga agcggttagg acactcatgc
    17581 catttcctaa aaaggttcta atccaaggca ccaataattg taggccgaaa aaaccataaa
    17641 caatagatgg aatggccgcc atcaagttga tagctgattt taagaagcca tagacgggct
    17701 ttggacaata ataaaccata aacaccgatg tcaagatgcc tgttggcacc ccaatcacaa
    17761 tcgctcctaa ggtaattaat aaggaaccaa cgatcattgg taaaatacca tagcttgccg
    17821 gaatgttcgt tggcgaccaa tcactgccta ataaaaaacg ggcaaagccg tagttagcca
    17881 tgaaaggtaa gccattacta aaaataaaga aacagattag caaaatagct acaacagcta
    17941 ctgttgcact catgaaaaaa attgccctaa aaactgcttc tttgaaggct tgttttatca
    18001 catcttgtcc tttctagtga agaaagtaag ggagatacga cacctcccta cttgccttct
    18061 ttatcttatt gtacgatgaa acgtctgcat ctctttagag atttatggag caaacatttt
    18121 atttaatctt gtcccaggtg gttaatttgc cactaaaaac gtctgcaagt tcagccatac
    18181 tgacttggct tgccttattg tcattattga ccacaacagc aataccgtct aaagcaatag
    18241 catcatgggt gagactctta ccttcttcag gagttaattc cctagaaacc ataccaatat
    18301 cagcggtttt ctccttaaca gcggtaatac ctgctgaaga cccattagag gtaatatcaa
    18361 tcgtaacttc tggattttct tttttataag cttctgctaa tttttccatt aaagaagata
    18421 ctgaagtgga acctacaaca gacaacttgc ctgataagtg ttggcttgta tattctgtgg
    18481 tttcggtttt agcttcaata aatttattat ctgtgaccac ttgttgacct tgtttggagt
    18541 ggataaagct gataaaatct tgacctagct tggaaagatt agaagaccaa acaatgttga
    18601 agggacgttg aagagggtat tcaccatcta aaactgtgtc tcgactagcc ttgacaccat
    18661 caatctctaa agccttgaca gattttgtta aagatcccaa ggagatgtag ccgatagcat
    18721 tagcattccc ttgaactgct gagagaacac cttctgtact attttgaatc acagctgttt
    18781 tggcagtgtt gtcaattttt ttatcaccgt cttttttgag aatccctgtg atttctgtga
    18841 aggcaccccg tgttccagag ccattttctc gtgaaatcac ctcaatcgtt cctgaagctg
    18901 actgtttgga agcagctgac tgattgccac aggcaacaag cccaaatcct gataagccaa
    18961 tggctgcaag agtaagcatt tttttgaatt tcataataat cacctttatc tctatgtatt
    19021 tttcttgtgt aggcttacta catttatagt ctaacaagtc tttgtaaagg tttatccctg
    19081 attcatgtaa agattgtgta aagaatcaaa aaaagccact tttgaaaaat ggctgctcct
    19141 aaaaatagct ttaaaaatta ttagtcctgt gcgaaagatt ggttaggaag aaaaatcgtg
    19201 aagcaactgc ctctgccaag ctgactcgtc accgtgactt ggccacctaa taattgactg
    19261 agttctttga caatggcaag gccaagacca gtgccaccag tttgtctgct tcgaccttta
    19321 ttaactcggt aaaaacgttc aaaaatacga tcctgctcta attgactaat accaatccct
    19381 gtatctgata cagaaatctt aatgccttcg ttcacctttt gggtcttgac ctcaattttt
    19441 cccccttgtt cagtgtaacg gatggcattg gataaaagat tgagtaagat ttgggaaagt
    19501 aattgactat ctgatacgag ggtgacatca tctggcacct gcacctttag ctgtaaatcc
    19561 ttcttcttga gctgaggttg caagctttga gtcaaatcct gtacaaattc tgccaaagaa
    19621 agggtcgtcc attgtatagg catttgttga gccttagata aggtaagaag atgctcaaca
    19681 atatgctcaa gacgcaaact ttctttgtaa ataatgtcta gaaagtcatc cttgagcgct
    19741 tcttcttcag ctgacatccc cttaatggtt tcagcaaagc ccttaatcga agtaactggt
    19801 gtcctcaatt catgggaggc atttgagaca aaggctaaat ttaacttttc ataagttcta
    19861 atcgttgtta aatcatatag caagacgagc acagcttcca cagattgggt ggggctaaaa
    19921 acgggaactg ctgtcacttc taaaatcaag tcaccctcat gaaacccact tacttcttgt
    19981 tttaaccttg ttttttgatc aaaggcttgg tgaactaaat tccgaatatc catccgtttg
    20041 aggtcatcaa gtgaacttat gtcgccgtcc acatcgggaa aataatgagg cagagagcga
    20101 ctggataata acatctgacc ttgagcggaa actaaaaacg tccccatggt taggtgcgac
    20161 agaagaacct ccattgtttc ggctagatcc ttgtattgct gatcctgttg ggagactttg
    20221 gtttttaggc cagacacata ctgagccaaa gactttaagt cttcttgccc tttttctaaa
    20281 aagtattcac tactggtcaa gagaggttgg tgcaaggtct caaaagcaac ttcccatttc
    20341 caaaggcaaa agagccagta gccacctagt cccaaagaaa gggctagaag aaagagaccg
    20401 atgcctttac tgatccaagt taatgccatc cctgcaatca gaatgaggct aacacttaga
    20461 ttgactagcc aaaattgaag gtagcgtttc atctataact ccttgaactt ataaccataa
    20521 ccccgaatgg ttcgaataaa ttgaggggct ttaggattgt cttcaatttt ttccctcaac
    20581 ttaccaatat gaacgtccac caaacgtgtt tcctgcccaa agtcataccc ccagatacgt
    20641 tccaaaagac gctctctagt cagtgtcatg ttgggatgtt tcataagata gagcaagagt
    20701 tcaaattctt ttggggtcaa actcagtaac ttattcgcct tgtagacttc atgacgctca
    20761 gggtatactt tcaaggtccc aaatagccaa gaatcgtcag cgatattatc tgaatcatct
    20821 ccttcttgtt ctcctttagt tcgcctgagg acagccttga cacgcgccag caattctcta
    20881 gggctaaaag gcttggtcag gtagtcatca gcccctaatt ccaaggccaa aaccttatca
    20941 aattcatcac ttttcgcaga aaccatcata attggagttt tgacgccttt ggctctcagc
    21001 cgcttacaaa cttccatgcc atctaattgt ggtaacatga tatcaagcaa gataaaatca
    21061 aagggttctg tttctgccaa agctaaggcc ttccgtccat ttgtcaccaa ttgagtagaa
    21121 aagccttcct tacttaaatg gtagtcaagc aatttcagaa tgtgttcttc atcatccact
    21181 aataagactt gttttgtcat ctattatctc ctattggtaa cattataaca caattatcag
    21241 aaatcctaac attgctaaat cagattaaat ttgcctatca agactagtat ctggtcaaac
    21301 gctcaatcat ctccttgtgc tctggatagg tcgccagtag atctaccctt tcaaataatt
    21361 caaaatcctc aaattcaaaa ccaggagcaa caagacaaga aaccagagca tcatccttat
    21421 caactgttga tccccaaata gtgcccttag gaacacagta gtgaagttgt tgccctttgg
    21481 atatgtccag gcctaaagtg actgcttcgt agtgaccatc tgctgtaatc atgtgaacag
    21541 taagtgggga tcctgcatga aaataccaga tttcatctgc tgtcaatcgg tgaaaatgtg
    21601 aaggattcgt ttcttctaat aagaaataaa tactggtata aagcgccctt cccttaccag
    21661 caaggtttat agtgtctgaa gctttttttg tttgtctaaa atagccacct tcaatatggg
    21721 gagctaactc tagagttctt atcaagtctt ctttatccgt cggagccaat gggttgaagt
    21781 aactcttgtt caaagtggtt ttacgatttc aagaactcct ctcagttctg aggaca
//
LOCUS       DAVAAL010000022        20949 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVAAL010000022 DAVAAL010000000
VERSION     DAVAAL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20949
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.022"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(46..1935)
                     /locus_tag="VQM60_000686"
     CDS             complement(46..1935)
                     /locus_tag="VQM60_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET1080575.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRGA
                     KAADEKRIQARGIQLKSSFEAVLKEMQEGVRKRVVFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2073..3638
                     /locus_tag="VQM60_000687"
     CDS             2073..3638
                     /locus_tag="VQM60_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET1080576.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYVSAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3638..4378
                     /locus_tag="VQM60_000688"
     CDS             3638..4378
                     /locus_tag="VQM60_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET1080577.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4487..4702)
                     /locus_tag="VQM60_000689"
     CDS             complement(4487..4702)
                     /locus_tag="VQM60_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080578.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5573..6823)
                     /gene="ilvA"
                     /locus_tag="VQM60_000690"
     CDS             complement(5573..6823)
                     /gene="ilvA"
                     /locus_tag="VQM60_000690"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET1080579.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6873..7229)
                     /locus_tag="VQM60_000691"
     CDS             complement(6873..7229)
                     /locus_tag="VQM60_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET1080580.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(7467..7703)
                     /locus_tag="VQM60_000692"
     CDS             complement(7467..7703)
                     /locus_tag="VQM60_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080581.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(7810..8832)
                     /gene="ilvC"
                     /locus_tag="VQM60_000693"
     CDS             complement(7810..8832)
                     /gene="ilvC"
                     /locus_tag="VQM60_000693"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET1080582.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8898..9374)
                     /gene="ilvN"
                     /locus_tag="VQM60_000694"
     CDS             complement(8898..9374)
                     /gene="ilvN"
                     /locus_tag="VQM60_000694"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET1080583.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9367..11067)
                     /locus_tag="VQM60_000695"
     CDS             complement(9367..11067)
                     /locus_tag="VQM60_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET1080584.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11267..12934)
                     /locus_tag="VQM60_000696"
     CDS             complement(11267..12934)
                     /locus_tag="VQM60_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET1080585.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(12937..13302)
                     /locus_tag="VQM60_000697"
     CDS             complement(12937..13302)
                     /locus_tag="VQM60_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET1080586.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13459..13647)
                     /locus_tag="VQM60_000698"
     CDS             complement(13459..13647)
                     /locus_tag="VQM60_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET1080587.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13763..13939)
                     /locus_tag="VQM60_000699"
     CDS             complement(13763..13939)
                     /locus_tag="VQM60_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET1080588.1"
                     /translation="MDLDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENVTQDLI"
     gene            complement(14050..14248)
                     /locus_tag="VQM60_000700"
                     /pseudo
     CDS             complement(14050..14248)
                     /locus_tag="VQM60_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
     gene            complement(14675..16219)
                     /locus_tag="VQM60_000701"
     CDS             complement(14675..16219)
                     /locus_tag="VQM60_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET1080589.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16432..16734
                     /gene="gatC"
                     /locus_tag="VQM60_000702"
     CDS             16432..16734
                     /gene="gatC"
                     /locus_tag="VQM60_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET1080590.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGIDRDRLFKNVPEKDNYYIKVPAILDNGGDA"
     gene            16734..18200
                     /gene="gatA"
                     /locus_tag="VQM60_000703"
     CDS             16734..18200
                     /gene="gatA"
                     /locus_tag="VQM60_000703"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET1080591.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKHSEETIYQVAAAFEATTGYHKQQPVIFGGDN"
     gene            18200..19642
                     /gene="gatB"
                     /locus_tag="VQM60_000704"
     CDS             18200..19642
                     /gene="gatB"
                     /locus_tag="VQM60_000704"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET1080592.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19766..20809
                     /locus_tag="VQM60_000705"
     CDS             19766..20809
                     /locus_tag="VQM60_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HET1080593.1"
                     /translation="MATMKAARWHAAKDVRIQEIEVPEVLPHQVKVAVKFTGICGTDL
                     HEFLDGPIFIPTDEHVYSGQKAPVTLGHEFSGEIIEVGSDVTRVKVGDRVAVEPILAK
                     NNLVGDYNLDPNLNFVGLAADGGFAKYCVLDGDLVHVIPDSLSYEQAALTEPAAVAVY
                     AVRQSSLKAGDTAVVFGLGPIGLLIVEALRAAGASKIYAVELSPERQAKAEELGAIVV
                     RPEEGETAVEAIHRLTNGGVDVSYEVTGVPVVLGQALAAVHKAGECMVVSIWEREANI
                     NPNEFAIQEKTLKGIIAYRHIFPKVLELMEQGYFSAEKLVTKKIKLENIVEEDFIELT
                     QDKSQIKILVEPE"
BASE COUNT         6171 a         4763 c         3835 g         6180 t
ORIGIN      
        1 ctatcttcca ttatatcata ctttttcagt ctatggtagc tctctttaaa aggctccgat
       61 actgccgcca cctccgcctc cagagaagcc accaccagaa cttccacttc cagaagatac
      121 agagtaggta cttgctgtat ttgcgacact agcataatgg ctcatttgcg cgcttgaatg
      181 ataaaacata ctgtgccagc cataagctac atagagattg atatctggat tttcaacttg
      241 aatctgatga accttcatca aatgactaac cttgtccgca tagccaaata aggttgcata
      301 gaccaagaga cgattccaaa caacaatact ttccaattca gccttatcca gtcgtgcaat
      361 ctcacgtaac atattttcaa aactggtcca gagatagtag acctcagctc ccgcttcatt
      421 tagaacacca tcacgattat ctagtcgaag cttccaataa tagaaaacag acaaaactaa
      481 ccctagaaaa ccaagtattg gcaaagggag gtaaagatag ccatgaacgt ctaaactgta
      541 caagaacaaa ccaaatccga taaatagggg caagatagtc aagacaccca tacccacttg
      601 caaagccttt tccaaaccag ttaaaggacg atagtaatct gggagtcccc agaagacaac
      661 tcgctttctc actccttctt gcatctcttt taatactgct tcgaaagaag atttgagctg
      721 aatccctctt gcttgaatcc gtttctcatc agcagcttta gctccgcgat aaaggctgtc
      781 agataccttg taatccgcaa acaaattgga aagagtggct tcttttttgc ctgaaaaggc
      841 tagatttaga cagtctctct caaatcttga caaaccatct tctttcacca atctcaagcc
      901 aactgcatct ccttctgaaa taatagaaac attcccacgg tccatcacat ctagcaaggt
      961 agcttgaata agttggtcaa aagtgaattt gccagccccc ttgactaagg gactcacttc
     1021 ctccaaggag gtcgagtaga cagcctctga taaaaccata ggctctaatt ccattggtgg
     1081 ttcatagaga cgatgatttt tggcatattt gactgaagga gtggtctttc ttctataaat
     1141 aaaatagaag cagacactca ataacaagga gatagaaagg atcgaaggga ggacccaagt
     1201 aacaagttgt ttactctgat ctttttctct aacaatcgag tcttctatct tattaaactc
     1261 ttctaaacga ttccctttca atccctgatc cctagcgcta gcaaaatcgg tccgaggcca
     1321 gtaggcatgc aactcaactc cacgcttagc cggaagattg tctaaacgga tagtataatc
     1381 aaggttactc ttttcaatcg ttccctctct aaaaagtttc cctgtatgga aaaagagttt
     1441 ttcagccccc ttgtctcccc ttacatgaaa ttcaaacttt tcaatagact ctgaactatc
     1501 tgtcagaggt tgccaattta attcagcgat atcatcataa aggaaaagta aattttttaa
     1561 gttccagacg aggtcaactt caactatgtc gccctcctga cctggattat agactctcac
     1621 agtataacca tccgcttctt ctgttacttc gctagtcaca tctgctagtt ctgcaccgtt
     1681 tttcgcggcc tgaacctttg gatgagggtc aatgtcaaac ccgctaggca tcttaccagc
     1741 acgtccaagt cccacgattt gtcccttaaa gtcctcctca aactggtaaa ctatcttctg
     1801 tctaaactct gccgtattat ctgcatgaat atacaaatcc ccttgataag agtttatctt
     1861 gaaatcaatg gcaaaaacag agagtggaag aaggcaaaac aagcctaaca ccagtaagaa
     1921 aaaagttttt ttcatcaact aagccccctt tttatctttc ttatcattat agcatttttt
     1981 cgtaagtaaa ggcttactta tgttgaaaaa ttacgcattt ttcgataaaa taggagacag
     2041 ttatttttta atagatcaga ataggagaaa tcatgagaaa aatatactta tctattttca
     2101 caagtctctt gctgatgcta ggacttgtca atgttgctca agccgatgaa tatttacgca
     2161 tcgggatgga agcagcatat gctcccttta actggaccca ggatgatgat agcaacggag
     2221 ctgtcaaaat cgatgggacc aatcagtatg ccaacggata cgatgttcaa atcgccaaga
     2281 aaatcgctaa ggacttaggt aaagaacctt tggttgttaa aaccaagtgg gaaggtctag
     2341 tccctgccct tacttctggt aagattgaca tgattatcgc aggtatgagt ccaactgcag
     2401 aacgcaaaca agaaattgcc ttttcgagca gttactacac tagcgaacca gttttgcttg
     2461 tcaaaaaaga ttctgcctac gtaagtgcta aatctttgga tgactttaac ggtgcaaaaa
     2521 tcacttctca acaaggggtc tacctctacg acttgattgc acaaatccca ggtgctaaaa
     2581 aagaaacagc catgggagac ttcgctcaaa tgcgccaagc tcttgaggct ggtgtcattg
     2641 atgcttatgt ttctgaacgt ccagaagcac tgactgctga agctgcgaac tctaagttca
     2701 agatgattca agtagaacct ggtttcaaaa ctggggaaga agatacagct atcgctatcg
     2761 ggcttcgtaa aaatgacaat cgtattagcc aaatcaatgc cagcattgaa accatttcaa
     2821 aagatgacca agttgccttg atggatcgta tgatcaagga acaacctgcc gaagctacaa
     2881 caactgaaga gactagcagt agtttcttta gccaagtcgc taaaattctt tctgaaaact
     2941 ggcaacaact cttgcgtggt gctggtatca ctcttttaat ctctatcgtc ggaaccatca
     3001 taggtctcat tattggactt gccattggtg tcttccgtac tgctcctctc tctgaaaaca
     3061 aagccattta tggccttcaa aaactagtcg gctgggttct caatgtctac attgaaattt
     3121 tccgtggtac gccaatgatt gttcaatcga tggttatcta ctatggaact gcccaagctt
     3181 tcgggatcaa ccttgaccgt acactagctg ctatcttcat cgtttcaatc aataccggtg
     3241 cctacatgac tgaaatcgtc cgtggtggta tcctagcagt tgacaaggga caatttgaag
     3301 ctgcgactgc tcttggtatg acccataacc agaccatgcg taagattgtc ctacctcagg
     3361 tagtccgtaa catcctacct gcaactggta atgaatttgt catcaatatc aaagatacat
     3421 ctgtattgaa cgttatctct gttgtcgaac tttatttctc aggaaatacc gtggcaacac
     3481 aaacctatca atacttccag acatttacaa tcatcgccgt gatttacttt gtcctcacct
     3541 tcaccgtaac acgtatccta cgctttatcg agcgcagaat ggacatggat acctacacta
     3601 caggtgctaa ccaaatgcaa acggaggatt tgaaataatg acacaagcaa tccttgaaat
     3661 taaacacctc aaaaaatcct atggacaaaa cgaagtgcta aaagacattt cactcactgt
     3721 ccacaaggga gaggtcatct ctatcatcgg aagctctgga agcggaaaat cgaccttcct
     3781 acgctccatt aacctacttg aaacaccaac tgatggacaa atcctttatc atggacaaaa
     3841 cgtcctcgaa aaaggctatg acctcacgca ataccgtgaa aagttgggga tggttttcca
     3901 atcctttaac ctctttgaaa atctcaatgt tcttgaaaac acaatcgtcg ctcagacaac
     3961 tgtcctaaaa cgcgaacgca cagaagctga aaagattgcc aaagaaaacc tggaaaaggt
     4021 cggcatggga gaacgctact ggcaagccaa accaaaacaa ctctcaggtg gtcaaaaaca
     4081 acgtgtggcc atcgctcgtg ccctctccat gaatccggac gccattctct ttgatgaacc
     4141 aacatcagct ctcgatccag aaatggttgg agaagtcctc aaaatcatgc aggacctggc
     4201 tcaggaaggc ttgaccatga ttgtcgtaac ccatgaaatg gaatttgccc gtgatgtctc
     4261 tcaccgtgtt atctttatgg ataagggcgt gatcgctgaa gaaggtaaac cagaagacct
     4321 cttcaccaat cctaaagaag accgaacaaa agagttcctt caacgctatc tcaaataaaa
     4381 agaaaaggct gcatcaaccg tgcagtcttt ttgctgtcct caaaatgaaa aggcagattc
     4441 tctatctcta ctacttgaag gtcaagaatc cgccgttatc caaagattaa tcttcaaatt
     4501 tttcataatt tttttcatag aaatcaatta ggcctagggc tgtttcaaat gaaatatttt
     4561 ttacttttgc acgaccctgc gccagagcaa tgatagacat ttcacgcgca ttcgtttctt
     4621 tagagatacg gtagcctgtg attttcttat cacgaaccca gccaacaact gattctactt
     4681 tttcaaagtt tgacttagcc atgtttttct cccattttct tctttataat atttaattgt
     4741 atacgttttt actacttttg tcaataaaaa aaacatatat tcaacgaaaa taattagttc
     4801 agaattttct tacttccttt ttaaatccga taatatatag gtttcgctag cctctacacc
     4861 ataaatattt gctaataaaa atactgtatt attaccctct taaggtgcga aattattcaa
     4921 gttcttaaaa aatgcttctc tatttccgta agatagctta tttcttcgat ttaagaactg
     4981 aagatgatag ttctaacctt tagaaatcca agccttcact agctcaaaaa ctcctatctt
     5041 tcttcctcaa taaatcatct gagagatagg tattccttat gtccttcaaa ttcatcactc
     5101 ctttggatat tgaggtgcta gcctagaagc agacacccga ttatctaaac catccttaat
     5161 aaagatgaca agacagataa gctcagttaa ttacttatat atctttttaa tatggtatat
     5221 ttgtcaacaa aaagaaatca aatcatacct tatactcaat gaaaatcaaa gagcaaacta
     5281 ggaagctagc cgcaagttgc tcaaaacact gttttgaggt tgcagataga gctaacgtgg
     5341 tttgaagaga ttttcgaaga gtattaaaga cagtgtgact atgcttgtca atagatagga
     5401 aatcaaaatg aaggatggaa atctgagcca aacatcataa gtgctataat gaacagaagt
     5461 aagaattgca cgataagttt tctaaaacca taggaatcgt cccctttctt catcttcatt
     5521 atagcacata tacatcagtt gtacaaataa tatgatattt ttttattagt cctcagacaa
     5581 gcatattata aagcgtttca ttaccattta agttaatata agctggatca aaaccttcca
     5641 ttctacgaat caaacctgca taatcatgct tatctgctaa agcgatccca attaatactg
     5701 ggcctgttcc cttgctagct cgtttgatat actcaaaacg tgtgatatca tcatttggcc
     5761 ccaggatatc atttacaaac tcacgcaaag ctcctggacg ttgtgggaaa ttgaccacaa
     5821 agtaatgttt gataccatca taaatcaagg cacgctcttc catttctggc atacggttga
     5881 tatcattatt tcctccagaa atgatacaac aaatggtttt ccccttaata tattcagcta
     5941 aaacctctaa agaggcgata ctagctgctc cagcaggttc tgcgactatc ccttgcttag
     6001 agtaaaggtc aatcaaggtt tcagaaatca atccctcatc gacacctacc aaagtttgaa
     6061 tatgttgacg agttgcttca taggtcaact gacctacctt ttgcacagca atcccatccg
     6121 caaatttatc aatttccttg agtttgactg gacctccagc ctcaaaggca gctttcatgg
     6181 aacgcgctcc attcgcctct actccgataa cctcaatctc tggacttgtt tccttgatat
     6241 aggtagaaac cccggcaatg agaccgccac caccaacagg aaccaagaca gcatcaaaat
     6301 caatcgattc ttttcgagct tcttctaaaa tctcataagc aactgttcct tgacctgctt
     6361 gaacatgagc atcatcaaaa ggatcaataa aggtacgatt ttcagagact gtaaattctt
     6421 gagctgcttt ggctgaggca tcaaaggtat ctccaactag tttaatagtt acaaaatccc
     6481 caccaaaaaa gcgaacctga ccaatctttt gttgtggcgt agtaatgggc ataaagatag
     6541 tagcaggaat tttcatttca ttacaagtat aggctactcc ctgcgcatga tttcccgcag
     6601 aagcgcaaac taccccacgt tcacgttctt ccttgctgag ctgggaaatg gcataatagg
     6661 caccacgaat tttaaaggag cgaacacgct gggcattttc ttttttcaaa taaatcttag
     6721 caccatactt ctccgataaa taatgatcgt aatccagtgg agtattcaca accacaccgt
     6781 tcaagacctt gtgagccttg attatatctt ttgaacttaa catataaaac ctctttttct
     6841 ataaaaaaca gtcactaatg actgtttttt agttattcat ctccgaaaat ttcagtaaca
     6901 ctttcatggt atttatgagc catacgaatc gaaaactctt taggaagttc ataacgattt
     6961 gtggaaatcc aatcatataa ccattctacc aaactttctt cactatcatt acgatggaag
     7021 ataaaatagt ttaaaatttc tttatattct acatcattca aatccaacgt gtctacaaat
     7081 tgactacgag gaacaaaatt agaatctaat tttcttagtt gatctgtcaa ttctcccaat
     7141 tgtaaatcca actgaaaaaa tagtttttgc agttgcctat cagtttcatc acatcctgtc
     7201 ttgatagaaa acaaattggt aatattcata tgatttcaac ccctaaataa gtacatctaa
     7261 tatcccacga atagctgaag ctaaagtttt cgcagtccag tcatttaaac ccgattcttt
     7321 caatccagat gcaataattt gctctatttg atataaaccg ccatctatcg tctcatttat
     7381 tctgcgaaga ggaccttcaa tataggataa agcttctccg aatctaacag ctgatttagg
     7441 tcctaatgat ttaccaacta tatttgttaa tattttttat ttctcaaaat ccatttcgct
     7501 atataacgag cagatttagt cgcaccagct ctctcaaacg gagaatgaat ttcaagacct
     7561 tcttctctag ctatttgtgc caatttgaag aattgttcaa aggtcatatg caacctacca
     7621 tcttcaaaat tagcatcatc ataaatgtta attacagcat tttcatcata cgaccaatct
     7681 gctttatttt ggtctgctag cactattacc gacggtgtaa ttacattata caactagaac
     7741 caataataac gatgcaattt ttctcataat ctttttcata aaatcatctc cagcgctata
     7801 tatttctaat tagttataga ttttgaatgc atcatcgtcg tttttaccaa cgaatggcat
     7861 tgctttacgc aattctgcac caactttttc aatttcaagg ttagctgctt gttcacggta
     7921 agcagtcaat tttggacgtc cagctttata gtcatttaca aagtcatttg caaatttacc
     7981 attttggatg tctgccaaga cagccttcat attttcttta acttgttcag tgattacacg
     8041 tggacctgat acatagtcac cgtattcagc agtgtttgaa atagattgac gcattttctt
     8101 gaatccacct tcgtagatca agtcaacgat caatttcatt tcgtgaagaa cttcaaagta
     8161 agccaattct ggagcgtaac ctgcttctgt caagacttcg aaacctgctt cgataagggc
     8221 agtcaaacca ccacaaagta cagcttgttc accaaacaaa tcttcttcag tttcttcttt
     8281 gtaagttgtt tcaagaagac ctacacgagc cgctccaaca cctttacacc agtccatagc
     8341 aatgttttta gcatttcctg ttgcatcttg gtatactgca taaagagctg gaacaccaaa
     8401 tccttcttcg taagtacgac gtaccaagtg tcctggtcct ttaggagcac acatgaagac
     8461 atctacatcc gcaggaactt tgataaattc aaagtggatg ttgaaaccat gggcaaatcc
     8521 aactgcgttt ccagcttcca agtttggagc gatttctgct tcgtacaatt cttgttgaat
     8581 ttcgtctggc gccaagatca taataacatc agccaactta gtagcttctg ctactgtgta
     8641 agtatcaaat ccatcttctt ttgctttatc aaaagattta cctggacgta caccgataat
     8701 aacgtcacga cctgaatcac gcaagttttg agcatgcgca tgcccttgtg aaccataacc
     8761 gataacggcg atttttttac cgtcaagtgc tgctacttta acatcttttt catattccat
     8821 ttgaactgtc atagcttttc tctcttttct attatttatt gccttttagg ctggtttaat
     8881 aaatttaagt tggattttta atcgcgggta aatccagttg cacccgttcg agcaatattg
     8941 cgaataccgt atgggcgaat gactcgcaat agggcttcgc tcttttctgc atttcccgtc
     9001 atctgaatgg taatcgagct tggcgctacg tctactactg ttgcacggaa aggttgaata
     9061 atcgctaaaa tctcagctct cttctcagct ggcgctgaca tcttaaccaa aatcacctcg
     9121 cgctccaaat gaggcttgtc tgtaatatct cgaatgcgaa tcacatcaat ctgacgattg
     9181 agctgtttga tgatttgctc cacttcatca tgagaagcaa catcaataat aatagtgata
     9241 cgcgatacat tcggatcttc tgttgctcca acagagatgc tttcaatatt aacctgacga
     9301 cgagatagga cacctgtaaa gcgattgagg actcctgaac gattttgtag ttttgctgtt
     9361 aacattctac gcatggaact gcacccccaa catctcatga ttactcttac cagccggtac
     9421 cattggtaac acctgttcct tacgagaaat atctacctca attagcatag gaacatcctc
     9481 agtgatgact tcaaggtctt gagccaaggt ctcaggattg tcaaacttat agtttttaat
     9541 accataagcc tgcgccatca attggaaatc aggaagggta tcaaagaccg actctgatgt
     9601 tctgccttca tagaaggatt cctgccactg gcgaaccatt ccaagtgaat gattgttcag
     9661 cataaccacc ttgattggca ccttgtaaat attcaaaata gccaactcct ggttggtcat
     9721 ttggaaacca ccatccccaa caaacaagac tacttcctta tctgggttag caattttagc
     9781 accgattgct gctggaattc caaagcccat tgttcccaaa ccacctgaag tcactaactg
     9841 acgttcattt tggtagggat aatactgagc tgtccacatt tggtgttgac caacgtctgt
     9901 taccacaatg gcatctccat tcgtcaattc accaattcgt tcaataactg cttgcggttg
     9961 aaccacacgc tctttcttat cataagaacg aacacgattc ttgtctttag tgactttctc
    10021 aatccacttt tcagtgttgt tgtgaactgt tggttctgct agcaacattt gcaaggcctt
    10081 cttagcatct ccaactacag gaatgtctgc actgataatc ttgccaatct cagctgggtc
    10141 aatatcaatg tgggcaacct tagcattctt agcgaaagtc ttaggattcc ccgtcaaacg
    10201 gtcatcgaaa cgagaaccaa tactaatcat aaagtccgct tccgtcatag caatatttgc
    10261 tgcgaatgac ccgtgcatgc ctcccattcc aagaaagagt gggtgactcg ttgcaatcgt
    10321 tccttgtccc aaaagactgg ttaccactgg aatttgatag cgttctgcaa attcatttag
    10381 ttccgtagca gcctcagcat aactaattcc accaccagct aacaagactg gctttttagc
    10441 cttggacaat tgcttcaaga ttttcttgat ttgcatatca ttcggctcaa gagtcggctg
    10501 ataacttggt aaattcactt ctggtgaata aatgaagtct gtttctaaag cagatatgtc
    10561 ttttggtagg tcaattacaa ctggccctgg acggcctgta gttgcgatat ggacagcttc
    10621 cgtaatgata cgcggaatat cagctgtctc acgaacttgg taattgtact tagtgattgg
    10681 catggtaatt cccacgatgt ctgcctcctg aaaggcatcc ttcccaatcc ctgctcgcgc
    10741 cacctgacct gtaaagacca aaaggggaac gctatcgctc atggcatccg caatccctgt
    10801 aatggcattt gttgctcctg gtccactagt gacgacggca acacccaact ttccagttga
    10861 tttggcataa ccttcagctt catgcaaaca accttgctca tggcgcccta gaatgtggcg
    10921 aatgccttta aaattatata tcgcatcata aaaaggcaaa accgcaccac caggataacc
    10981 aaagatggta tcaactccta aatcacgaag tgtttccaaa actaggtccg accccgtctt
    11041 aggagattct aaactgattt tctccattat tcccctttct ttcctcttaa aaataacttg
    11101 ttactatcat accatttttt caaaattttt caagataaaa gaagaaattt tctgaatttt
    11161 ctattttaac gtttatttat gaatgttatt tttgttttta ttaaaaagat accgatttca
    11221 tttactaaaa agaaaaaaga actgatttct cagtccttca ttaatcttat tccacactaa
    11281 ataggtatgg gtaaacaggt tgttgacctt ggtgaatctc gacttcaacg tcttcgaatt
    11341 cttctacgat ttcttgagcg atttcattgg caagttcttc gcttccgtct tcacctacat
    11401 agaaggttac gatttcactg tcttcatcca acatatgttt caaggtttca gtcaatgttt
    11461 ggtgcatatc agggtttgac acaagaattt ttccatccac catacctaaa ttatcgtttt
    11521 catggatttc taagccatcg atcgttgtat cacgcacggc tgttgtgacg cttccgctaa
    11581 cgacatcgct aagagcagct gtcatacgct cttggttttc ttcaatggac ttgcttggat
    11641 caaaggcaag aagacttgtc ataccttgag gaagagtgcg agcctctacc actaccgctg
    11701 gttgctccaa aacttctgcc gcagattgag ctgccatgaa gatgttcttg ttgtttggca
    11761 agaagatgat gttacgggca ttaacctgtt caacagcctt gataaagtct tctgttgaag
    11821 ggttcatggt ttgaccgcct tcgataacat aatccacgcc ttgagaacag aagatatctg
    11881 ctagaccttt accagccacc acagcaatca aagcatactc tttttcttca gccgacttga
    11941 taacttgagt agcttctttc tcaacctgtg cttcgtgttg gttacgcata ttgtcaactt
    12001 ttaccttgac caagctacca tatttgagac cttcttgcat aacaagtcct ggatcttctg
    12061 tatgaacatg gactttgaca atttcatcat cgttaacaac gaggagagaa tctccaagct
    12121 catccaagta gttacggaat tcatcgtagt caaaatcttt agcataggtt ggaccttgct
    12181 taagagctac catgatttca gtacagtaac caaacgtgat gtcctcagtc gctacgtgac
    12241 cagctacaga cttatgatgc tctacattga tcatctcact catgttggca ggagtcgcta
    12301 caaagtcctc agatgcaata tattcgccag taagggctga aaggaaacct tcgtagatga
    12361 agaccaatcc ttgaccacct gagtccacaa cgccaacttc tttcaatact ggaagcatgt
    12421 ctggtgtttt agctagagct gttttagcac cttccaaggc tgcgcgcatg acttcaacag
    12481 cgtcatctgt ttgctcagct tttttcttag caccgatagc agctccacga gaaactgtta
    12541 aaatcgttcc ttcaacaggt ttcatcactg ccttataggc aacttccaca cctgattgga
    12601 aggccagagc caagtcttga cctgttaact cgtctttatc cttgatagct tgggaaaatc
    12661 cacggaaaag ctgagacgta atcactcctg agttcccacg cgcacccatc aaaagccctt
    12721 tggcaagaat gctcgctact tctccaactg tagaagctgg cttgtctgca acttctttag
    12781 caccattttc aatggtcatt cccatatttg tcccagtatc tccatctgga actggaaaga
    12841 cgtttaatga attgacatat tcagcttgct tattcaagcg agttgatgca gcctgcacca
    12901 tttcttgaaa taagctagta gtaatttttg acacgattat tctcctacaa ctttgatatt
    12961 ttgaatgtag acatttacag tctgagcagt aattccaagc tggttttcca agctaaaggc
    13021 aacacgctct tgaatgtttt ttgacacttc actaatcttt gttccgtagc ttaacacggt
    13081 atatacatca actgcaatac tgccatcttc ggctgccttt acgacgacac ctttagaata
    13141 attttcctta cctagcaggg cttggaaatt atctttgagg gcatttttac tagccatacc
    13201 gaccacacca aaaatctcag ttgctgctcc acctacgacg gttgcaatca cttcatctgt
    13261 tagttcgatt tgaccatctt ttgtattaat ttttacagtc atccttttta cctcaactag
    13321 ttgatactct attttatcat atttcagccc aagtgtaaaa gcagaatgat gtatcagcgg
    13381 atatttactc tatttttcaa acgatgttat acccacaata aaagaaaaaa gaccctaagg
    13441 tctcctttgc ttttattatt aaacgcgttc aactttacct gatttcaaag cacgagctga
    13501 agcccaaact tttttaggtt taccatcgat aagaacagta actttttgaa ggtttggttt
    13561 tacggcacgt tttgtttggt tcatcgcgtg tgaacggttg tttcctgata cagtcttacg
    13621 acctgtaaag taacatactt tagccattgt gttttcctcc tattagatct aatatagcgg
    13681 atgtgctagc accacatacc gtactatgtt atcacacttt cttgtttttt gcaagggaat
    13741 tggaagattt tttatttgtg tcttaaatca ggtcttgcgt gacattttct gctctccaca
    13801 tgccattgtt gattaacaga acaccagaat taaaattatg tgtataaaaa tcatctctaa
    13861 ctgcagctaa gggtatagcc gtcaagtcca aatcccacag ctcatctatc gattttctta
    13921 caacaatatc taaatccaaa tacagtacac gagactcgct tacatacttt ggaataaaat
    13981 atctaaaaaa gccgcatatg aaagtccctc aaaggggaga cgataacctt tcagaatact
    14041 actgtcaatc taaacattca caatctcact attcaaagtc tctagtcttt tttccatcaa
    14101 ttggaaccat tctcgcggaa ggtcatcatt aaaaacataa aacttaagat tataatagat
    14161 gaacacaaag agattttatt gttgtttcaa ctttatccat ataagcatta tctgcaccta
    14221 agacaatcgc ttttttctct tctttcactt tttatctcat ttctttttat tcccatcata
    14281 tgtttctacg taaccattat tttcgcctat tcgttcgtaa aaccatacca gtggagattt
    14341 tagatgaagt cccattacgg tttacaattt ttacattacg acacggagtt ttacaaatcg
    14401 atttcatttg ccaaacgtag ttagtaaggc agttagctag gctacctact ttctgctacg
    14461 acggaaaaat ccacaaatca ataccattcg tggatttttc ctagtgaagc gtttaggtaa
    14521 gccgccatag cggttaccga ttattagatt acgacacgga gtttggcaaa tcgattctat
    14581 ttgccaaacg tagttagtga ggcagttagc tagttcgcca aatagcgact agcgtccaac
    14641 aattggaact ttagttccaa ttgttggtac tgagtcacat cttctcctct aactctacgt
    14701 ctggatactt gtccgcaaac cagcggaggg caaagtcatt ttcaaagaga aagactggtt
    14761 ggtcaaaacg gtctttggct aagatattgc gacttgacga catccgttca tccaagtcct
    14821 caggcttgat ccaacgaacg gtctttttac ccattgggct catgaccact tccgcattgt
    14881 attcgccttc catacggtgt ttaaagactt caaactggag ttgaccaaca gctcccagca
    14941 tgtactcacc tgtttggtaa ttcttataaa gctgaacggc tccttcttgc accaattgct
    15001 caatcccctt gtggaaggat ttttgcttca taacattctt agcagaaact ttcatgaaaa
    15061 tttcaggagt aaaggttggc agtggttcaa attcaaactt gttttttcca accgtcaagg
    15121 tatccccaac ctgataagta ccggtatcgt aaaccccgat aatatcacct gccacggcat
    15181 tgatcacatt ctcacgactt tccgccataa actgggtaac attagatagt ttagccccct
    15241 taccagtacg agggagattg acactcatgc cgcgctcaaa ttcgcctgat acgatacgga
    15301 caaaggcaat acggtcacgg tgacgaggat ccatgttggc ttggatttta aagacaaagc
    15361 ctgagaaatc cttgtcataa ggatccacaa tttcaccgtc tgttttcttg tgaccatgtg
    15421 gttctggagc aaacttgagg aagatttcaa ggaaggtctg cacaccaaag tttgtcaggg
    15481 ctgaaccgaa aaagacaggc gtcaattctc cagccagaat agcttcctct gaaaactcat
    15541 tcccagcttc atttaaaagc tcaatgtcat ccttgacttg ctcgtagaaa ggattgctac
    15601 caaagagttt gtccccgtct tctagactgg caaaacgctc atcccctttg taaagctcta
    15661 aacgttggtt atagaggtca tacaagccct caaaggcttt ccccatcccg ataggccagt
    15721 tcatagggta gctagcaatg cccaagattt cttccaattc ttgcaagaga tccaaaggct
    15781 cacgaccgtc acggtccagc ttgttcataa aggtaaaaac tggaatgcca cgatgtttca
    15841 caacctcaaa caatttcttg gtttgagcct cgatcccctt ggcagagtcc acgaccatga
    15901 ccgcagcatc caccgccatc aaggtacgat aggtatcttc tgagaagtcc tcgtgccctg
    15961 gcgtgtctaa gatattcacg cgcttgccgt cgtagtcaaa ttgcataaca gatgaagtaa
    16021 cagaaatccc acgttgcttc tcgatatcca tccagtcaga tttagcaaaa gtccctgttt
    16081 tcttcccttt taccgtacca gcctcacgaa tctcaccccc aaagtagagt aactgctcag
    16141 tgatggttgt tttccccgcg tccgggtggg agataatggc aaaggtacga cgtttcttaa
    16201 tttcttcttg aatattcata agttctcttt ctttgattct ctatttttct tgtttcaata
    16261 gctgagaatg atttttacat tggattttac cattcctttc aacactccat tatatcggat
    16321 tttagcattt ttttcaattt ctatttcttt tcacttcccc ctcccttatt tataggaaaa
    16381 tatggtaaaa tagaacagac taaaaatcat catttcacga aaggatgcaa gatgaaaatt
    16441 acgcaagaag aggtaacaca cgttgccaat ctttcaaaat taagattctc tgaagaagaa
    16501 actgctgcct ttgcgaccac cttgtctaag attgttgaca tggttgaatt gctgggcgaa
    16561 gttgacacaa ctggtgtcgc acctactacg actatggctg accgcaagac tgtactccgc
    16621 cctgatgtgg ccgaagaagg aatagaccgt gatcgcttgt ttaaaaacgt acctgaaaaa
    16681 gacaactact atatcaaggt gccagctatc ctagacaatg gaggagatgc ctaatgactt
    16741 ttaacaataa aactattgaa gagttgcaca atctccttgt ctctaaggaa atttctgcaa
    16801 cagaattgac ccaagcaaca cttgaaaata tcaagtctcg tgaggaagcc ctcaattcat
    16861 ttgtcaccat cgctgaggag caagctcttg ttcaagctaa agccattgat gaagctggaa
    16921 ttgatgctga caatgtcctt tcaggaattc cacttgctgt taaggataac atctctacag
    16981 acggtattct cacaactgct gcctcaaaaa tgctctacaa ctatgagcca atctttgatg
    17041 cgacagctgt tgccaatgca aaaaccaagg gcatgattgt cgttggaaag accaacatgg
    17101 acgaatttgc tatgggtggt tcaggtgaaa cttcacacta cggagcaact aaaaacgctt
    17161 gggaccacag caaggttcct ggtgggtcat caagtggttc tgccgcagct gtagcctcag
    17221 gacaagttcg cttgtcactt ggttctgata ctggtggttc catccgccaa cctgctgcct
    17281 tcaacggaat cgttggtctc aaaccaacct acggaacagt ttcacgtttc ggtctcattg
    17341 cctttggtag ctcattagac cagattggac cttttgctcc tactgttaag gaaaatgccc
    17401 tcttgctcaa cgctattgcc agcgaagatg ctaaagactc tacttctgct cctgtccgca
    17461 tcgccgactt tacttcaaaa atcggccaag acatcaaggg tatgaaaatc gctttgccta
    17521 aggaatacct cggtgaagga attaacccag aggttaagga aaccattcta aatgccgcta
    17581 aacactttga aaaattgggt gctattgtcg aagaagtcag ccttcctcac tctaaatacg
    17641 gagttgccgt atactacatc atcgcttcat cagaagcttc atcaaacttg caacgctttg
    17701 acggtatccg ttacggctat cgcgcagaag atgcaaccaa ccttgatgaa atctatgtaa
    17761 acagccgaag ccaaggtttt ggtgaagaag tgaagcgccg tatcatgctg ggtactttca
    17821 gtctttcatc aggttactac gatgcctact ataagaaggc tggacaggtc cgtacactta
    17881 tcattcaaga tttcgaaaaa gtcttcgcgg attacgattt gattttgggt ccaactgctc
    17941 caagtgttgc ctatgacttg gattctctca accatgaccc agttgccatg tacttagccg
    18001 acctattgac catacctgta aacttggcag gactgcctgg aatttcgatt cctgctggat
    18061 tctctcaagg tctacctgtc ggtctgcaat tgattggtcc caagcactct gaggaaacca
    18121 tttaccaagt tgctgctgcc tttgaagcaa caacaggcta ccacaaacaa caacccgtga
    18181 tttttggagg tgacaactaa tgaactttga aacagtcatc ggacttgaag tccacgtaga
    18241 gctcaacacc aattcaaaaa tcttctcacc tacttctgcc cacttcggaa atgatcaaaa
    18301 tgccaacact aacgtgattg actggtcttt cccaggagtt ctgccagttc tcaataaagg
    18361 ggttgtcgat gccggaatca aggctgctct tgccctcaac atggacatcc acaaaaagat
    18421 gcactttgac cgcaagaact acttctaccc tgataatccc aaagcctacc aaatttctca
    18481 gtttgatgag ccaatcggtt ataacggctg gattgaagtc gaactagaag acggtacgac
    18541 caagaaaatc ggtatcgaac gtgcccacct agaggaagac gctggtaaaa acacccatgg
    18601 tacagatggc tactcttatg ttgacctcaa ccgccaaggg gttcccttga ttgagattgt
    18661 atctgaggca gatatgcgtt ctcctgaaga agcctatgct tatctgacag ccctcaagga
    18721 agttatccag tacgctggca tttctgacgt taagatggag gaaggttcga tgcgtgtgga
    18781 tgccaacatc tcccttcgtc cttatggtca agagaaattc ggtaccaaga ctgaattgaa
    18841 gaacctcaac tccttctcaa acgttcgtaa aggtcttgaa tacgaagtcc aacgccaggc
    18901 tgaaattctt cgctcaggtg gtcaaatccg ccaagaaaca cgccgttacg atgaagcgaa
    18961 taaagcaacc atcctcatgc gtgtcaagga aggggctgct gactaccgct acttcccaga
    19021 accagaccta cccctctttg aaatttctga cgagtggatt gaggaaatgc ggactgagtt
    19081 gccagagttt ccaaaagaac gtcgtgcgcg ttatgtatct gaccttggtt tatcagacta
    19141 cgatgctagt cagttgactg ctaataaagt cacttctgac ttctttgaaa aagctgttgc
    19201 cctaggtggt gatgccaaac aagtctctaa ctggctccaa ggggaagtcg ctcagttctt
    19261 gaatgctgaa ggtaaaacac tggaacaaat cgaattgaca ccagaaaact tggttgaaat
    19321 gattgccatc atcgaagacg gtactatttc atctaagatt gccaagaaag tctttgtcca
    19381 tctagctaaa aatggcggtg gcgcgcgtga atacgtggaa aaagcaggta tggttcaaat
    19441 ttcagatcca gctgttttga ttccaattat ccaccaagtc tttgcggata acgaagctgc
    19501 tgttgccgac ttcaagtcag gcaaacgtaa tgcagacaag gccttcacag gattccttat
    19561 gaaggcaacc aaaggccaag ccaacccaca agttgccctt aaactacttg cacaggaatt
    19621 ggcgaagttg aaagaaaact agacagaaca aaaccagccc taaggttggt tttttcttga
    19681 caaatagaaa gaaaacgttt gcaatattgc gtgtaaagtt tccaaacaag aaaactaaaa
    19741 atcgcaacaa taaggagtta tttatatggc tacaatgaag gcagctcgct ggcatgcagc
    19801 gaaagatgtt cgtattcaag aaatagaagt tcctgaagta cttccgcacc aagtaaaagt
    19861 tgcagttaag ttcacaggaa tttgtggtac tgacctccat gaatttttag atggacctat
    19921 ctttatccca acagatgagc acgtctattc tggtcaaaaa gcaccggtaa cactgggaca
    19981 tgaattttct ggtgaaatca tagaagtcgg aagcgatgtt acccgtgtta aagttggtga
    20041 tcgtgttgct gtggaaccaa ttctagctaa aaataactta gttggtgatt ataatttgga
    20101 tccaaacctt aattttgtcg gtttggctgc agacggtgga tttgccaaat attgtgtttt
    20161 agacggtgac ttagtacatg tcattccaga tagtttgagc tatgagcaag ctgctcttac
    20221 tgaacccgct gctgttgcag tctatgctgt tcgtcaatct tctttgaagg ctggcgatac
    20281 agctgttgtc tttggtttag gcccgattgg tctattaatt gtagaagctc ttcgtgcagc
    20341 tggtgcttct aagatttatg ctgttgaact atcacctgaa cgtcaagcaa aagctgaaga
    20401 gttaggagct atcgttgtcc gtccggaaga aggtgaaaca gctgttgaag ctattcatcg
    20461 tttaacaaac ggtggtgtag atgtttctta tgaagtaacg ggtgtgccag tagttttagg
    20521 acaagcctta gctgccgtac ataaagcagg tgagtgcatg gtggtctcta tctgggaacg
    20581 agaagcaaat attaatccga atgaatttgc aatccaagaa aagacactaa aaggaatcat
    20641 tgcttatcgt catatcttcc ctaaagtatt agaattgatg gaacaaggct acttctctgc
    20701 tgaaaaatta gttactaaga aaattaaatt ggaaaatatc gtcgaagaag actttatcga
    20761 attaactcaa gataagtcac aaattaaaat tttggtagaa ccagaataaa aaatcaatct
    20821 ttttaagaca atactaacag gtcggagtaa acttacttcg gccttttgaa atataatact
    20881 cttcgaaaat ctcttcagac cacgtcagcg tcacattacc gtatatatgg ttactgactt
    20941 cgtcagttc
//
LOCUS       DAVAAL010000023        20439 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVAAL010000023 DAVAAL010000000
VERSION     DAVAAL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20439
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.023"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQM60_000706"
     CDS             complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQM60_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET1080594.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQM60_000707"
     CDS             complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQM60_000707"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET1080595.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTSLTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4065..4628)
                     /locus_tag="VQM60_000708"
     CDS             complement(4065..4628)
                     /locus_tag="VQM60_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET1080596.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4713..5267)
                     /locus_tag="VQM60_000709"
     CDS             complement(4713..5267)
                     /locus_tag="VQM60_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET1080597.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(5278..6501)
                     /locus_tag="VQM60_000710"
     CDS             complement(5278..6501)
                     /locus_tag="VQM60_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET1080598.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6538..8988)
                     /locus_tag="VQM60_000711"
     CDS             complement(6538..8988)
                     /locus_tag="VQM60_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET1080599.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLLALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQKNDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSCRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9188..10117)
                     /locus_tag="VQM60_000712"
     CDS             complement(9188..10117)
                     /locus_tag="VQM60_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET1080600.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10257..10544
                     /locus_tag="VQM60_000713"
     CDS             10257..10544
                     /locus_tag="VQM60_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET1080601.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFSAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10578..11912)
                     /gene="ciaH"
                     /locus_tag="VQM60_000714"
     CDS             complement(10578..11912)
                     /gene="ciaH"
                     /locus_tag="VQM60_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET1080602.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11902..12576)
                     /gene="ciaR"
                     /locus_tag="VQM60_000715"
     CDS             complement(11902..12576)
                     /gene="ciaR"
                     /locus_tag="VQM60_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET1080603.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12685..15231)
                     /locus_tag="VQM60_000716"
     CDS             complement(12685..15231)
                     /locus_tag="VQM60_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET1080604.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVCSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15354..15794)
                     /locus_tag="VQM60_000717"
     CDS             complement(15354..15794)
                     /locus_tag="VQM60_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET1080605.1"
                     /translation="MTPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15769..16638)
                     /locus_tag="VQM60_000718"
     CDS             complement(15769..16638)
                     /locus_tag="VQM60_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET1080606.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16616..17116)
                     /locus_tag="VQM60_000719"
     CDS             complement(16616..17116)
                     /locus_tag="VQM60_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET1080607.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17127..17825)
                     /locus_tag="VQM60_000720"
     CDS             complement(17127..17825)
                     /locus_tag="VQM60_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET1080608.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17818..18084)
                     /locus_tag="VQM60_000721"
     CDS             complement(17818..18084)
                     /locus_tag="VQM60_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET1080609.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18150..19076)
                     /locus_tag="VQM60_000722"
     CDS             complement(18150..19076)
                     /locus_tag="VQM60_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET1080610.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19151..19966)
                     /locus_tag="VQM60_000723"
     CDS             complement(19151..19966)
                     /locus_tag="VQM60_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080611.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSIFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(19970..20290)
                     /locus_tag="VQM60_000724"
     CDS             complement(19970..20290)
                     /locus_tag="VQM60_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET1080612.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6151 a         4483 c         3629 g         6176 t
ORIGIN      
        1 gttaccaagt tactcaaagt acagatttga agctgcagat aagactgacg aaatcacttt
       61 aatactaaat gaaaatcaaa aagcaaacta agagcctcag gctgtacttg agtacggtaa
      121 ggcgacgctg acgtgatttg aagagatttt cgaagagtat aaaacactgt tttgaggtgg
      181 tggataaatc ggctgggcaa aaactcaatc tcaggagaaa atgaagtaaa tctccccaca
      241 actatagaaa aagatttgat tctgctcctc acacaataaa atctttttct tttattaaaa
      301 acgaatcgtt tcacgtgttt tctcatatga ggtaacaaag cgattggtta caccaggctc
      361 tgccacttcc aatgcttgag aaatcttgtc aaatgaagcg tgataatcaa attctttttc
      421 aaaaatatct aaagcttcgt taaatgcttc ttgaatgcgt tcatcaaatg agcgatagcg
      481 gttagaatat tgcaagagtt gctctgtcaa agttgcatat tgtacaatat tataagtttc
      541 cgtttctaaa gcttccatat cattcgttgc aatttcaaga actcgggtaa cagattcaat
      601 gttaatcatt ttttgttcta actcaaccat taaatcctcg gtattattgc ttgccgtaaa
      661 gaataacttc aagaaagttt gtggaatacc tggcagattg cgtttttcca tgtatcgctt
      721 gatagtatgg agacgattga cataaacatt ggccttttga cgtgcattaa tatcatcttt
      781 ctcaatttgt gtcaggcgct cactaactga aatttgctca tcttcaatat cttttagttg
      841 agtttgtaaa tcctcaagat tttcttcaag aactgaataa gcttgggttg gttcttcttg
      901 atttgaagtt acctcaacaa tagctgcctc aaaactctct aattctgtct gaatacgacg
      961 aacatggctt gcagctgtct caggaagtaa ataggtcttg ttcaaacgtg caatatcttc
     1021 tcccaataaa gtattattct ctttcatatg ttgaaggtaa gttggaagag ttgcaagtag
     1081 attttccact actttctgag cagcaatttc tcgagtaaaa atatcataca aggcattgat
     1141 ttcctcttgt gcctgtccat tctcatattc ggcattatcc aattccaact gacgaatatt
     1201 ctcttggttt ttcttgaatg cttcataaag caagtggaaa cgcgcttcaa tatccgtttc
     1261 aacaaaatga taattagcat caattagttt acgataaccg gcttccaaat cctgtaattg
     1321 atctggcaat tctgtagaaa gcgtcgtaac caaggctgga acacgatcca caatatgact
     1381 taaggccaaa atgtgatttt ctgtattatc caaaatcact gcggcttcca cagggtcacc
     1441 cgatgaattc aaggttacaa attgtgaaaa ttcagattgg atattttcta attgtttttc
     1501 aatttcatcc aaggcttgac catactgttc tgaattttca gcaactctat gctgaagttc
     1561 ctcaaataaa tccaaagcat gaagaacacg accactattt ttagattctt gcttctctaa
     1621 gtctgccaaa gcattgcgaa ttgccgcaat atcttcttca atcaaagtaa tttgactctc
     1681 aatttggtca atttgatgac tggccttgag aaaacgaaat gaatggttat agccttctgc
     1741 ttcaaagaga ttattttcaa tatcggcaaa agagttgaga gataaatcga cccatttttg
     1801 attccattca cggaaagcca cttgactttg tccaatcaag tgcatatttt ttacagcttc
     1861 tacttcatca tttactggaa gattgtatag ttcttctttt ctttcttcta gcgcctctaa
     1921 tctcccctcg tttcgcttac gtagaaagat tgccactaca taagccagaa ctaaaatgac
     1981 tgcaattgca accattaaat aaattagttg tccattagac atatcaaact ccttttttac
     2041 ttgaaacaat cgtaacgatt atatcatatt ttttagacca agggaactac ttctccctat
     2101 tttttagaca tcaagtgtac tatagacagc attttcttcg ataaactcac gacgaggctc
     2161 tactcgatcc cccatcaaca tatcaaagat tttatctgct tctgcagcat catctacaga
     2221 aactctagcc atcaagcgat gttcgggatc catggttgtt tcccacagct gatgatcgtc
     2281 catttcacct agccccttat aacgctgaat agtcggtttg gtacgacctt cactataacg
     2341 ggctaaagct tcttggagtt tgatttcttg atctgcaccc ggctggatat attctttaat
     2401 ctcgcttcca accttgacac catagattgg tggttgggca atataaacat aaccagcttc
     2461 taggattggt ttcatataac gataaatcaa ggttaaaaga agggtacgaa tatgggctcc
     2521 atcgacatcg gcatcggtca tcaaaacgag tttttggtaa cgggctttcg aaacatcaaa
     2581 ttctgcgcca aatcctgttc ccatggctgt gaaaagacta cgaatttctt cgttagctag
     2641 aatcttatcc atacttgctt tttcaacgtt caaaatctta ccgcgaattg gaaggatagc
     2701 ctgaaactca cggttacgac cagatttggc tgatccacca gctgagtctc cttcgacgat
     2761 gaagagttct gtttcagcag ggttattaga agaacagtct gctagtttcc ctggaaggtt
     2821 ggaaatttcc aaaccagatt ttttacgtgt gacttcacgc gcacgcttgg cagccacacg
     2881 agccttggca gctaaaatcc ctttttccac gatacgtttg gcaatctgtg gattttccat
     2941 gaggaaatcg gagaaggctt cactgaagag gcgattggta atcttgacca cttcgctatt
     3001 tcccaatttg gtcttggttt gtccttcaaa ctgtggattt gggtgtttaa ctgagataac
     3061 tgcagttaag ccttcgcgaa catcttcccc tgttaaatta tcttcattgt ctttcagtaa
     3121 cttattctta cgagcataat cattgataac acgagtcagt gacgtacgga aaccttgttc
     3181 atgcgttcca ccttcatggg tatgaatatt attggcgaaa ctcatgacat tttcatggta
     3241 acctgttgtg tactgcatgg ctacctcaac tgtgatatca tccatctcac cgtctgtata
     3301 gattggtgta tcaaagatta catccttgtt ctcgttgata tattcaacgt aactagcaat
     3361 cccaccttca taatgataat gcttggtttg ttccaaacct tggcgcttat cagtgataga
     3421 aatttgaaga ccgcgattta gaaaggccaa ctcttgaatc cgtttattta atttatcaaa
     3481 atcaaaggtt gttgtttcag taaagatttt tgggtccggt atgaagtgaa ccgttgttcc
     3541 agttctatct gtatccccaa ccacctcaag gtctgcaaca acatgaccac gacggtattc
     3601 ttggtaatga atcttaccgt ttttatggac atgaacgtct aattgagtgg aaagggcatt
     3661 aactactgac gaccccaccc cgtgaagacc acctgaaacc ttgtatccac caccgccgaa
     3721 ctttcctcca gcgtgaagga ctgtaaagac ggtctcaaca gcaggacggc ctgttttttc
     3781 ctgaatatcg actgggatac cacgcccatc atccacaaca gtaatcgaat catctggctc
     3841 aataaaaact tgaatatggc tggcaaatcc tgccaaggcc tcgtcaattg agttatcaac
     3901 aatttcccag actagatggt gaagaccttc ttttgaggtt gatccaatgt acatccctgg
     3961 acgcatacga acagcctcta agccctctaa aacttgaatt tgactggcat cataatcctg
     4021 tgcctgcaga tttttgattt cttctgtcat cttattcctt tttcttatat gtctaatact
     4081 tgtcttaaat tcacgatact ggtaaacaag tgggtatcaa gtcctgcagc ttgacctgct
     4141 tcgatatcaa tcggccgatc accaatgaca agaccagagc taatctgata cttttctctt
     4201 aaataaagca tggattcggg atttggcttt ctcttaaagc ctgagctaga agtcaccact
     4261 tctgtaaaat aagctgctat agaggttttt tctaaaattt ccaaaacctg atcatttcga
     4321 tgagagacca aaaaatgacg gccaccttga tttgaaatgt cttccaatag gtcagaaact
     4381 ccttcaaata aaatcgggtg ttcaagctct ctggcttcat tttccttgta cttttctaaa
     4441 aaattctcta aattgggagc gaatgtctca atcgcaaaag gagtagaaac ctttaaagct
     4501 tgatagacac tgtcatggtc ttgtgtgata ccatacagtg ccaatgtttc aacaaatgca
     4561 gctgttgaag tttcataatt atccagtaaa gttccaccta aatcccagat gtaatcgtga
     4621 tatttcatac ccttcattat agcatttttt tatgaaaaaa gcgatgattt ccctgagttt
     4681 tccacataaa aaacgagaga ataactcccg ttttactgat ttttaccaaa gacatcttga
     4741 tagagcattc ctgttcgtaa actctctgcg tcccctccta gttgctccac caactcgtag
     4801 gcaaaggcaa gggctgttga aggaccccga ctggttgtca actgaccatc taccactact
     4861 gtttccttga cgtagtgacc atcaaggatt tgctcttgaa cgccgtcata acaagtgtat
     4921 cgcttatttt tcaatatctc tgcttgattg agggcaattg gtgccgcaca aatggctgct
     4981 agtttcttcc cttcttgctc gaagctttgc aattcttgaa tcaaggtctg attatcacgt
     5041 aaatgtgcag aaccaggcat acctccagga agaacaatca tatcatagtc tgataaatct
     5101 ccatcaaaga catgatctgc tcttacttgg attgcatgcg aacccgttac ttgctcttca
     5161 aaaccaacca tatcacatgt gatattggct cgacgcaaga catctacaac tgtcaaggct
     5221 tcaatttctt caaagccctg agctaacata actgctactt ttaccatgat tttctcctta
     5281 tttaatttgt tttaatacaa cctttttccg tttgaatggg acttttccgc tcttttcttc
     5341 agctagatta gtctggtaac tcatcgataa aagcaaacca acaccaatca gattactgat
     5401 aatagctgat cccccttgcg aaatgaaagg caagggaatc cccgtcaaag gaagtagtcc
     5461 agtcacagca ccgatattct caaagatgtg gaagagcaac atcataatca aacctgtgga
     5521 aatataagtg tagaactggt tatttgattt aagagtaatc ttcaacatac ggtaaatcaa
     5581 catgagatag agggcaataa ccaggacaga gccaataaag ccaaaatctt ctgcaataac
     5641 cgtaaaaatc atatctgact ctcgaactgg gataagcaga ttcgaagcat taaatccctg
     5701 accaaataag ccaccactcc caatggcaat ctgcccttga gcctgctggt aagtcgttgt
     5761 ttgggcaaac tcaaagggat tgagccaagc caaaatccga ttgatttggt aggtcggcat
     5821 tccaatctgg tgaagaaaag ctcgtccgtc cttgctaata aagatagcta agaaaccagc
     5881 aactcctgtt acagcagtca caaatactgg gataataatt ttccaagaaa cccctgataa
     5941 taaaacgatt cctgagaaaa tggctacaaa aaccaaagcc gtccccaagt cactttgaag
     6001 tgctaaaaga actaggactg gaatggtaaa gagaatcatc cagaaaatta acaaaaagtc
     6061 cagcggaacc gtgcgtctcc attccttatg tttctttgta aattggacaa tgacacgagc
     6121 caacatgagg atataggata tcttcataaa ttctgacggt tgaaataggg taattccatt
     6181 tattgatacc cagtttttgg cacccgttga tgcaactaag cttggattat aaaatacaat
     6241 cggcaagatc ataagtccca agcctaaaat atatagaaag ggggtcacct tccaaagaaa
     6301 ttctgtatta aagagcatga ccacaaaacc aatcacaagc cccaaggcaa tccaggcgac
     6361 ctgctgccct aaaatgggca gaatattatt gggataatca tgactaacgg ctatatagat
     6421 agccaccaca ccgatgacca gtagaaaaaa tactggcaag agcaaactat aatcgactct
     6481 agagtcgaga gaacgtttca tatatactaa ccttatactt tcatacaata ctatttatca
     6541 aagttcatta aaaaatctat caatagcctc gtcaacttcg gatcgagaga tggttttaac
     6601 agttgcttct tctgctagag atgctactat ttgtttgccg tatcgttttc cgatgattct
     6661 cctatccaaa ataagagtta aggaacgttg gtattcacgt ctcatacttc ttcccaaagc
     6721 ctgttttaaa cgaataatgg ccattggcaa ttgataatca taaaaggcat ttttcccttc
     6781 ttgattcagt tcttgattaa tctttttcgt caagggttct tgaggatttt ggaaaggaag
     6841 cctcggtaca acttgaatca caaaaggatg gcttgaaaaa tcaactccct cccagaaact
     6901 tgctgcacca agcaagattt gttgttcacc tttttcaaag cgtttcttta gctgatgaac
     6961 atccccattt ttatactggg ccaagtggct aactgtaagt aaatccgata ctgctagaag
     7021 catgtcttta gcggtaaaga gaaccaaaat cggttgctgg aaagcttgaa tttccactag
     7081 taaagcagct acctctctgg catagacttc taaggaggtt tctgttacca gagggaaatc
     7141 tttgaccata accacttctt gttcctgttt tccccgagat tcaatcttga caaatttggc
     7201 ttcaggatag cctaaaaggt ctgccaaaga aaccctctga ctaatctcaa gagtagccga
     7261 tactcccaag acttggcaac tctcaggaag taaagaggac agaagagtac ggcatgattt
     7321 tgtagaagaa atctgaattt tcttttgact cgtttcagtt acttcaagcc agtaatcacc
     7381 ttcagctgta aaatagcgaa ccagctcatc aaagccttct acttctaatt ctgaaaaata
     7441 ctgatggaga ttgtccagag aatctaagat atttttccta gatttaccag actgaaattg
     7501 ttctatcaag tagagacact caaagcgaat actttctagt atccgttgtt gaaccctgtt
     7561 ttcttctcct actaaagcct tatcaattaa atcgataata gattgtatat cgtaggtctc
     7621 ttgaagcaga ttttctagag ctaacaaaat cttttggact tcatcaataa tcagtaaacg
     7681 gtcactgaca aattcaggat tatcttcaag tctggtcacg agataggcat gattagtcac
     7741 taaaagcttg caagtctctg ccctttcttg actacgtttc caaaaatctt ccgtcacaaa
     7801 taagctctgg gatgataaat tcccatcatg acgaaggtct gctagaaaat gttggtaacg
     7861 gtagagttgc ccgatttcat ccaaatctcc tgtctctgtc tcagtaagcc agaccaagac
     7921 ttgcatttta aagcgtctaa ataagcgatt ttcatcattt ttctgcaagg aatgataaaa
     7981 ggcatccaac ttcagataat tttgtggtcc ctttaagcta tgaatatctg tatggaacac
     8041 ttccttgagg cgtttacctt cttcttccat gatttgattt tgaagaatct ttgtcggaac
     8101 actaagaaca atttgtcgct ctttggattg agataaagcg agtaagagat agccataggt
     8161 tttcccaatc cctgtcggcg cttgaatcag agagacaggt tcatctttca atagcaagcc
     8221 aacctcttta gcaaaacttt cttgttcctc cctcacttca aggttcaaca gagaaatatt
     8281 tttagaaaag tcttgagata gttttcgtgg ctccagagaa gctgccgttt tcttaaaata
     8341 cagaccttga acttggacca agtctggaga actcaggata gattggttgc gataagtttc
     8401 ctcaataacc aggtaggact catataggag agcgtcagcc atttccagca agcgttccaa
     8461 gagaccttta ggaagctggg tcatcttttc ccgtaaaaaa agaagtaatt ctgctgtagc
     8521 ttgggcatct gaaagggctg tgtgtgcgtg tttaagagga attcctaatt ctcgacacaa
     8581 aatcggcaag ctatattttt ccagttcagg gaaaaagacc tgggccaatt cgaccgtatc
     8641 aacacgaggg tttcttagct catagccttc aaaaaataaa ttttccgcca agagattagc
     8701 atcaaactga acattatggg ctacaaaaat cccatcctcc accaagtcaa atatttttct
     8761 ggcaacttgc gaaaaatcag gtgcttgcgc cagacgttgg tctgtcaatc ctgtcagttc
     8821 tttgatatga gcatccaagg gttcatgtgg attgacatcc gtcgtatagt gatcgacgat
     8881 ttctccgtcc tcaatcacga caattcccac ttggataatt ttagccttac tacctgtgct
     8941 agttgcctct aaatccacca caacatagcg attaccaatc gttttcatta taaaaaatta
     9001 taccaaaaaa agggccattt ggcacctgca aacataggat aattttcaaa ctcaagaaca
     9061 ctacttttct taaaaatccc atcgaataga gcattctagc ctgctgattt ttgtaaaata
     9121 gtgaagaaat acgaaaaatt gagagaagaa atgtttgcat cttccttctc tcaacgaatc
     9181 atgtatttta tctaaccatg tcaggatcgt agtcataaaa tcctgtatca aggaaacggt
     9241 ttttagggtg aagtttacga acttcttcat ccagcaagaa ggcttgatca tggctaaagt
     9301 tgccctcttg aatcaagtta cgcgcttgga taaagagttt tgcaatctca acaaagttct
     9361 gaccaggagt gtagagccct tctagtttcc agcgagtaaa gccatgctcc accaattctg
     9421 ttaatttgat catcaaatca aggtcattgt tggcaaagat atgggtccca tgattatctt
     9481 caaaaatgga atagtggctc tctggatcac ttggctcagc caagaagagg tcacgtttat
     9541 gcgtcttttc atcatcgata tgtgtaaagt tatagtagtt ttgcaagagt ggacgtttag
     9601 aatgatggat gacgctagca ccgtaaacca aaacttcagc aggaatttcc aaaatctctg
     9661 gcattttgaa aagttcagct gatggaattt cacgcgccaa aacagcctca gatgcgccag
     9721 ccttttgtcc ccagaagtta atctgacgac tgctagttac catggttgaa gcatcgtaga
     9781 tggtcttaaa tgaataacca tcgcggttaa ctacgtaaaa gacgcctgca tccccaatcg
     9841 taatatagtc tgtcttgatt tcttccaaga agtctaagaa aggcttgata cggtccatca
     9901 tatcttggtg catgagagca ttgaccgcaa cgatcaattc cttaccagca tcatgaacca
     9961 acttagcgat ttcacgtaat tggtcataac taaaggtcgt tggcagacga aggccaaaat
    10021 ctttctcacc gacatagata cggtctacgc cagcttcgag tagttgttca acttgttcaa
    10081 tactttcagc agttgctgta atgacaatct tttccataag aaaaattata ccacatttct
    10141 ccaaaatcat ccatgctttg aaaatgaaaa gggactttat tctttttgta acctttctgt
    10201 aataattctc tgccgaaaaa atgataaaat aggtatgtac tgttaaggag agaattatgc
    10261 ccgtaagaaa attacaatcc tatgaggtag actatcaaga agaattaaac cagcagcttc
    10321 ctcattacca agcttataca cctgaagcgc aatctgatgc caatctcaag gaaatcctat
    10381 tttttattaa tatcgctgtt ttttgtatct gtattgctat ctttagtttt atctttttag
    10441 cattaaaatt atcaactgct cttgcctttt ccgcagcaat cggattcagc ttacttgttt
    10501 taaaagttca acggtctatt atcaaacgaa aacgtagaag ataaatccaa aatatcgccc
    10561 cagttggagc gatattttta ttttttcttt ttagatggtg tctgaatggc aatcttcact
    10621 tcaaagattg ttcccttggg tttattatct ttgacagtaa cagttccttt tagagcatct
    10681 acaatttgct tggctaggga taatcctaaa ccaaaaccac ctttttgccg ggttctagct
    10741 ttgtctactc gataaaaacg gtcaaaaatt ttctttttat cttctgtcga aataccgatt
    10801 ccattatcag aaacaagtaa ataaagattg cgatcggtcg ccgagataag aaaatcaatt
    10861 tcaccatcct cctcagtata cttgacggca ttatcgaaaa gaatggtcat cagttgtttc
    10921 agaagaagct gatctgtgac aattgttcga tggatacgat tttcaaaacg gaagacacga
    10981 ttattttccg aagcaatcat ctcgtagttt gtgaaagttg tattaaaaaa gctagttgga
    11041 acttctgcaa gctccggctt aatcccatca tctctccgag ctaagttcag caagcttgtc
    11101 gttaaaaaac gcatatttcg gacttcttcc aaactcgatg caatgctttc gctcacatcc
    11161 ataatggtag cttctggctt acgaaaaagg gtctctaagc gattttgcaa aactgcgagt
    11221 ggagttcgta actcatgact ggcattttcc acaaaagact gttgcttctg catactctca
    11281 agcaggggcc taacactgac cctagctaga tagagactgg caagtaaaga caaaatccag
    11341 aaactagcca tcacgaccac aatcaattgc tcatgctttt gactagcctg ctccaactga
    11401 ctggtattaa tcaagacagc agcatacttg atattggttg aaaccgaact aatattggtc
    11461 tccatcaaaa tcacacgata gatttcttcc tgcccatagc tattaaaaac ctgaatctgg
    11521 tagatatgtc ctagttcttt cttttctaac ttaatcttat ccaagcccaa aaatcgattt
    11581 ccagaaagaa gttgagtaaa gtctttatca aagagaatga cttccgtatt ggaactgaca
    11641 ttaggtttta tttctacttt actagcgtcc gccctagcat tttctaaatc tttaatctct
    11701 tctgttgccc tatttatagc cagctgaata actgcttgag gattttcact cagtccatga
    11761 agcttatcgt ccaccgaagt ataaagactc gaatgcatga cttgtaaaat aatcagagtc
    11821 attgtagaaa aaatcagggt gaagacaccg aagttgcgga taaaataact aaagtcatcc
    11881 gcataccatg tttttttaag tttactgaac atcttttaaa agatacccaa cactacgcaa
    11941 ggtttgcaaa ttctctgcaa aagtggttcc ctttaatttc ttacggactt ttgaaacata
    12001 gacttcgaca accgaaatcg ttgtatcact atcaaatccc catagacggt caaaaatctg
    12061 cgtcttaggc aaaatcacat tttgattttg aaggaaataa actagtaaat cgaactcttt
    12121 ccccagcaat tcgacaggag tatcttcaac tttaacggta ttggttgata aattaaccac
    12181 gatattccca taagtcaagg tgttttcatt aaacttccct gaacgtttga gaagggcctg
    12241 aatccgcatt ttaagttctt ctaggtagaa aggtttggtc agataatcat ccgctcccag
    12301 ttcaaatcca tgtcccttgt catccaaact ttccttggca gtcataatca gaactggtgt
    12361 cgtaattccc ttttcacgca attcttttaa gacttggaaa ccattttttt ctggcaacat
    12421 caaatccagc aaaatcaagt catagacacc actctcagct tcgtagagac cttcttctcc
    12481 atcaaatacc tgcataacat ccgcaaaatc gtctaaaaag tcaaatactg aatttgacag
    12541 acctaggtca tcctcaacca ataagatttt tatcatgaga aactcctcct tattaaaact
    12601 attataccaa atttgcctta aaaaaaactc aactctctgc attttacatg agatagctga
    12661 gttttctttt tattttaggc ttatttatgc atttccgtat tgaagaacaa ctgcttcgac
    12721 tgcagctttt tcacggctaa tcaagtcaac acgcgctgca atttccttga ttcccatacc
    12781 gatgttacgg ctaagagcaa ggtcagaaag ttgcggttca aagaactcct tgtattccgc
    12841 caagcgttgc tgagtcttaa atacatgagc aggaaggata acaaagctat caaagctcat
    12901 atctcctcca agggctgcct taatccaagc ccagttttca cgcgcccaag accaagctgt
    12961 tttctgagtt gcttgatgag ctaggaattg gtaataccaa gcagacaagt cctgtggttt
    13021 gaccacaaat ttgtccttcc aagaagtaat caagttttgg atattatccg catctgtact
    13081 gtatgcaaga gctgctgcca actggcgttt aaagacagca tctgttgcgt gagtataagt
    13141 atcaagataa agtgctaaca agtctttagt ctcatgatgt ttcatctcat taatcagaac
    13201 ttgtgaacga atagctgctg ggagtcctgc aagattctcc ttgtgtgttg cgaagatttg
    13261 gctagcgact tgactagctt ctgcatcatt tgagcgaatc atcatcgaaa cagccagctg
    13321 acgaaccaat tcatcctcat ctgattctcc gtctttagct tcaaaaccaa gacggtcata
    13381 gttatgacga gccaatttag caaccagtcc tttgaaggct gtttcagcat ccgttccttc
    13441 atcaataaag cgctcaaggg ctgaaatcac ttgagaaaca gctgaaacca ccagataaga
    13501 ctcttcctta gcaagtttat caagaactgg aagcaagtct gcataagaaa tgtgccctgc
    13561 ctcagccaac aaacgacgtt cttgaacaat ttgcagtttg cttgtgttat caagtgtctc
    13621 tagctcagca agaacagctt ctaacaagtc tccttgatag tcggtaatat agtgggcagt
    13681 attttcagtg ttgagacgaa gagctccttc attttcagca agaagagctg cgtagccagg
    13741 gatttcgata ctttcagttt cgagtgtatc aggcaagcct ttccagttgc tattgagggg
    13801 caccacccag agacggttct tgtcttcgtt ctcaccgatg aagaattgtt tttgtgaaat
    13861 cttcaagaca tcattttcaa ctttgacagt aagaactggg taaccaggct gttccaacca
    13921 agaatccatg aaggctgcga catcacgtcc tgacgcttga ccaagggcat cccaaaggtc
    13981 actaccaatg gtattgctgt attggtgttt ttcaaagtag gcgtgcaaac ctttagcaaa
    14041 atcagcatct cctagccaac ggcgaagcat gtgcatgaga cggcttcctt tggcatagac
    14101 gatagcgccg tcaaagagtg tattgatttc atctggatgt ttaacttcga cgtggacaga
    14161 ctgaacgcca tcagtagcgt cacgttcaag agcaagaggt actccacctg tttggaaatc
    14221 ttcaaagata ttccagcttg gttcgatggt atccacacag acgtattcca tcatattagc
    14281 gaaactttca ttgagccaaa ggtcatccca ccatttcata gtcacgaggt tcccaaacca
    14341 ttggtgagcc aattcatggg ccacaacaag ggcaacttgt tgacggctag caaatgtaga
    14401 gttctcatcg acaaccaagt aaacttcacg gtaggtcaca agaccccagt tttccatagc
    14461 accagctgag aagtcaggaa gagcgatgtg gagagattga ggaattgggt acttaactcc
    14521 atagtaatct tcgtaaaact cgatagagca aacagcgata tccagtgaga aatcaagatt
    14581 tgaaagtgga tgtgctttgg ttgagtagac acctaccagg gtaccatttt tagttttagc
    14641 ggtcacccct tgcaaatcac cagcaacaaa ggccaacaag taagaagaca tgcgaggtgt
    14701 tgtctcaaac ttccagatac ctgtttcctt acggttttca acatcgattt ctggcatgtt
    14761 tgacaaggcc aattcacctt ctgcttggtc aaagcgaaga gagaggtcaa aagttgcttt
    14821 ggcttcaggc tcatccacac atgggaaagc ttcgcgcgca aaatggctct cgaactgagt
    14881 agacaagacc tccttcttga ctccatcaac tgtataataa gaagggtaaa tccctgtcat
    14941 gttgtctgta attttaccag aaaaggcaag aaccaattca acttgaccag cctcagccaa
    15001 ttcgatatga agggcttcat tgtcatggtc aactgtaaat ggacgagctt gacctgcaac
    15061 ttctacagag gtgatttcca agtctttttg gtggagggag atgcggtcac tctgtgcttg
    15121 accagtgatg gtcactttcc cagaaaaagt cttggtctca cgactcaaat ctaaaaataa
    15181 atcataatgt tcaggaacaa attgcttaat aaaatgttca actgcttgca tagttttctc
    15241 ctattctaag tttaagagct gctgctcttc ttcaaacaaa cttattatat catattttgc
    15301 ttgagaaaaa aggattggac agcgatttcc aaaactttct tccttctaat agtctacagt
    15361 gggtagacct tgcgaaattc ttctaaaaca accttgcttt caggtgtaaa agtcagccct
    15421 acttcaccca aacagtctgt gaaaaagtcc tcatgaacct ggcgccaata ggcaagtgat
    15481 ttgtctccct caccttcctt aaaggcatgt tgcgcagaaa cttgattgaa gagttcaaca
    15541 gaaacctttg taatttcgac aatgcagaca gactgatttt gactatctaa aatgacatcg
    15601 aaggtcccta cttggggaag tggttcgtct tctagtacat agaggtcata ggctgatgct
    15661 gttgctgtct tttctccttt aaacaccaaa tccgctaaaa ggtctggttc aactccaaaa
    15721 gcccaagcat cgatttcatc tccgatcaaa ggattgattt gtttgtattt attccacatt
    15781 tcttgcggtg tcatgggtgc tcctttgtaa ttttttactt tcttctttta tgtgtttaag
    15841 atgatctgga tggtcaatct ctaaatcaaa aatctctgga atagaactgt agtggataat
    15901 gcacttgata cccaactgat tcattttttg tatgaaagaa gtattcagat agcctgctac
    15961 agcaaaatta atcttgttct ttcttgcttt atcctgcata tctcttagca tatctaacat
    16021 tattggactt tccatatcat gccattgact gtttctcata gtcgcaaaaa caaaggaagt
    16081 caaatcattc attccaacta caatctttga aatgcccgtt tccagtatac tagataagtc
    16141 aaaatacgct gacggtaatt caatcatcgt tccgactttc ccagtaaagc catactgacg
    16201 caatactgta atagcttgtt ttaattggtc ggcatcattg acaaaaggaa aaataagaga
    16261 cagattggga ttggtttgat aaacttctgt aacgacatgt gcttcagcct gaaattcatc
    16321 caaacacgcc agtaaacgcc tggttcctct atagccaaac aagggatgcc cttcgtcaaa
    16381 aaactctcta gtccccgcta aacaattggc ttctgtattc gttaattcag aaaaacgata
    16441 ccaaacttcc ttatctaagt aaaaggagca aatagtatca agataatctt ttacaaattg
    16501 ctgacaactt gctaatatga tgttttgatt aagctctctc aacaaatatt ccccacgaat
    16561 catgccgacg tggtgaaata gttgaggata aattttttca agaatttttt cgccactaag
    16621 ggcaagttga tttctcatca ttcaccttcc aattcatgta agaagtcttg tccagttctg
    16681 gaaatcctaa taattcagac ttaaccttca agactaatgg cgatgcattt tcttctgtaa
    16741 tctcttgaat atccatccaa atatatccaa gtgaatcatt cgcaccatca gacacagctt
    16801 ccgaagtcgt aacttgaggt gcactctcat tcatttcaac atcatacaag gccatgacat
    16861 ggtgaaccat aaaatttgtt aactcttctc tgacgaaaac atcgtagatt cgaggattag
    16921 agtagcttct aacagtaaat cccgtctctt ccataacttc tctagtcagc gtttccgtca
    16981 gtccttcacc aagttgctga ctgcctccag gtagatcata ccgatgttga taagggcctc
    17041 tcgttttttc aatgcagagt aacttcccat tttctaagca aacagcgtag actccaaagt
    17101 gttttttgat ttccatccaa ctcctcctac ttcaaagacc agccaccatc tattgtcaag
    17161 atttgtcctt gcatggcgct cgcttttcca cttgctaaaa aaagactaag ctctgctatt
    17221 tcctctggct caatccagcg cttgattggg gtttcactag ccacccagtc agccaaacca
    17281 cctggttcaa aatccgcagc ggtcatagct gtcttgactg ctcctggagc gataccaaag
    17341 acctgaatcc cagcttcagc atagtctaga gccaactgct tggtgaagcc agccaaggca
    17401 tgcttggatg aagtataggc gtgaccacct ccacctgcta ggctagaagc aatggaacac
    17461 atattgatga tgattccctt tttattttcc agcatttgtg tcaaataata ccgagtcaac
    17521 tctactggaa taatgtagtt gatttcaaaa atctcttgaa tgtcctgcgc cgtttgttcc
    17581 aacagtggtt tgtaatcatc caaaactcca gcagtattac acaaaacatc cacctgaggg
    17641 caccagtcaa aaataggttc caagtccaag gtcaaatctc tctgtaaaaa gtgaaaatca
    17701 ccctctaaga gtggcttttc accttggtca actccataaa cttgatagcc cttctctaaa
    17761 aagaggcgag cttgagccaa tccgatccct gaactcactc ctgtaatgag tacacgttta
    17821 gtcatgcact tctacccaat ccgttgccaa aacatcacaa actgtcgggc tccacatgga
    17881 aaaaccttct ccttcgccag aaacgttgat taggaaataa ggtgtcattt caagtgcaag
    17941 cccattttgc tcgatggtat caaagagttg gacatagttt tccgcacctc cccaaccagt
    18001 tcgtacatat tttctcttag cctttaaccc aggcaggatc tcttcaaatg tcatgttttt
    18061 ctcctttaat tctacattct tcatttaatt atagcaaaaa accgctttat acggcttttt
    18121 gaatgtgagt tattcaaacc tgctactact tacggcaaat tattccctgc agcaagataa
    18181 atttcatacc attcttttct tgttaagcta aagtttgccg ctcggctaac ttctctcaag
    18241 tgcttaggat ttgttgtacc tacgactgcc tgcatttttg ctggataacg caatatccaa
    18301 gaaatggcaa tagttgaaga ggttactcca tatttaatag ctaaacgatc aagtacttga
    18361 tttaaagctt gaaatttctc atttccaaca aaatttcctt taaaataccc gaattgtaag
    18421 acagaccatg cttgaatgac cacatcgtgt aattggcaat attcaaaaat gctgccatct
    18481 cgcatagctg cttgactatc ttccatatta acatgaaaag ctgattcaaa tcctggagta
    18541 aaagccgcac tcaattgtag ctgattaaca gctaacggct gcttgacatc ttttttaagc
    18601 aactccatca tcataggatt ttgattagaa actccaaaat ctcgaacttt accttgttta
    18661 taaaggagat caaaggcttc tgctacttgg tcagattcca tcaaagcatc tggtcgatga
    18721 aggagcaagc tatctagatg atcaatcttc aatctttgca aaataccgtc tactgatttt
    18781 ataatatagt ccttagaaaa atcaaaatag gtaaattctt caatgcgaat gccacatttg
    18841 gactgaatcc acatcttttc tcttaaatct ggacgatttt ttaggacaag acctaacagt
    18901 tcttcacaac gaccacgacc ataaatatca gccaagtcga aggcattgat tccaacagaa
    18961 agtgctgttt ctacaagctc ttcaacttct tttacagatt tatcttttat tctcatcatt
    19021 ccgagaacaa tttctgataa ttctttgtca tcttgaccaa gagttatgta tctcatcaaa
    19081 tttttctcct ttaatttcta acattcttcc cttcattata acaaaaaacc gctttgcaac
    19141 gactttttga ctatacttca ctccatttta tcttcttaaa cccacggaac aagacaaaga
    19201 ttccaataaa gaggacagct aaaggaataa cttttgtaag gaaaacattt gaaattccca
    19261 tccactcata ataacggagc agagaaccca ccacaagatg ggcaataatc atactgacaa
    19321 atggacgaaa gaccgcttct ttccaattcc aaataccgat aactagcgaa atcgtaaaga
    19381 tagacaaact atcccaggga gccggaatat aaaaggctcc ttcttgtatg aagcttgcca
    19441 ttcctacata tcctaaaaca actagaagaa ctatagtccc aacaacaatg taagtgccaa
    19501 ttttcatttt aggagaatct tggactaaac ttcttcgtaa aattgtggcc acaagtccaa
    19561 atccaatcag aaaaataaga agttgcccta aaaatgtgag caaattgact gttaagagag
    19621 gacctttaga aaaatcactt agtagttgat aataacgtaa taccgccagg acaagaattg
    19681 gcgtcaaaag ggactctttg atagaactgc gaggtgctcc cttgagaatc tctttcatta
    19741 tttttttagg attcttacct agataatcct ctgcactcat gccatctcgt tctgcttctg
    19801 agaaatctag catcatcaaa tagatctgct ctctgagata gtcttcatca tagagaaatc
    19861 cagcaagatt aaaactttcc cacaactcct caaaatactt ttgattctcc tcagaaaact
    19921 catgtagcaa agcgcttgtt tcttcgtaat acttcatttt cttcatggtt taacccccat
    19981 tcttaatccc ttctactttt tgactcaaat cgtcccattg ttgccaaaag actgagacac
    20041 gctcttctcc ttctttcatt aatgaaaaat acttccgatc tggaccatct ggcgacgggc
    20101 gcatgtcgcc tcttatccat tgattttttt ctaacttttg caacaaagga taaatagttc
    20161 ctggaacgat agtatcaaat ccagcctctc gcaaagtctg aaccaactca taaccatacc
    20221 gctctttttg accaatcata tccaagacac aaccttcaag aacacctttt aatagctgag
    20281 tttctttcat cacttctccc ttctaatcta ttttgtaata cctactagta acttcaccta
    20341 tagtatatta cttctacact agtttgtaaa gcataatagt taatactctt cgaaaatctc
    20401 ttcaaaccac gtcagcgtcg ccctaccgta tgtatggtt
//
LOCUS       DAVAAL010000024        19388 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVAAL010000024 DAVAAL010000000
VERSION     DAVAAL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.025"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(282..650)
                     /locus_tag="VQM60_000725"
     CDS             complement(282..650)
                     /locus_tag="VQM60_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HET1080613.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGCNWWNSRGSFATVLDVDL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALTVTGFSLG
                     GAIAYGVDWADGSLDGYIFA"
     gene            1409..1729
                     /locus_tag="VQM60_000726"
     CDS             1409..1729
                     /locus_tag="VQM60_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET1080614.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDNAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            complement(2019..2489)
                     /locus_tag="VQM60_000727"
     CDS             complement(2019..2489)
                     /locus_tag="VQM60_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET1080615.1"
                     /translation="MRMPLKRPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVAVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQTGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(2455..3261)
                     /locus_tag="VQM60_000728"
     CDS             complement(2455..3261)
                     /locus_tag="VQM60_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET1080616.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(3468..4109)
                     /locus_tag="VQM60_000729"
     CDS             complement(3468..4109)
                     /locus_tag="VQM60_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080617.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(4106..6214)
                     /locus_tag="VQM60_000730"
     CDS             complement(4106..6214)
                     /locus_tag="VQM60_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET1080618.1"
                     /translation="MKKISHLCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(6269..6562)
                     /locus_tag="VQM60_000731"
     CDS             complement(6269..6562)
                     /locus_tag="VQM60_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET1080619.1"
                     /translation="MIGEINHEKIGVASLMVCSILASTSVLAVWVDGGQWNYGVGWSG
                     NFGYSDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
     gene            6969..7289
                     /locus_tag="VQM60_000732"
     CDS             6969..7289
                     /locus_tag="VQM60_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET1080620.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLVICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(7341..7928)
                     /locus_tag="VQM60_000733"
     CDS             complement(7341..7928)
                     /locus_tag="VQM60_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET1080621.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            8170..8841
                     /gene="sdaAB"
                     /locus_tag="VQM60_000734"
     CDS             8170..8841
                     /gene="sdaAB"
                     /locus_tag="VQM60_000734"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET1080622.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            8850..9722
                     /gene="sdaAA"
                     /locus_tag="VQM60_000735"
     CDS             8850..9722
                     /gene="sdaAA"
                     /locus_tag="VQM60_000735"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET1080623.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            9744..10376
                     /locus_tag="VQM60_000736"
     CDS             9744..10376
                     /locus_tag="VQM60_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET1080624.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIVELLQELSKNYPL
                     YITTTKNTPTAQDMTKNLGIHHFFDGIYGYSPEAPHKADVIHQALQIHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLEYFQ"
     gene            complement(10726..12576)
                     /locus_tag="VQM60_000737"
     CDS             complement(10726..12576)
                     /locus_tag="VQM60_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET1080625.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(12618..13775)
                     /locus_tag="VQM60_000738"
     CDS             complement(12618..13775)
                     /locus_tag="VQM60_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET1080626.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            13833..14999
                     /locus_tag="VQM60_000739"
     CDS             13833..14999
                     /locus_tag="VQM60_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET1080627.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            15138..15464
                     /locus_tag="VQM60_000740"
     CDS             15138..15464
                     /locus_tag="VQM60_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET1080628.1"
                     /translation="MYFPTSSALIEFLILAVLEKGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            15451..16044
                     /locus_tag="VQM60_000741"
     CDS             15451..16044
                     /locus_tag="VQM60_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET1080629.1"
                     /translation="MTRTEYLTQLELYLKKLPEADQIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHVALLALLAAPIGIPLGIAILV
                     SLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGDQHA"
     gene            16037..16948
                     /locus_tag="VQM60_000742"
     CDS             16037..16948
                     /locus_tag="VQM60_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HET1080630.1"
                     /translation="MRKLTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLAMSKEPVY
                     DSRMEELTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSESPFISSGIDGILHIVSSSSNRFNDVIIGLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNGYILRIEGSRIKNSKLTTPNIVNIFDTDLTDSQLESTEHHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDVSSNYGSIFQFTREEPESRGTELSNPYK
                     TEKTDVKDQLIARSDDDIELISTPSRR"
     gene            17011..18075
                     /locus_tag="VQM60_000743"
     CDS             17011..18075
                     /locus_tag="VQM60_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080631.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEERIADLDTPIEK
                     NTQVEEEVSQAEVELESQQEEKMETPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEALK
                     SPTSKLETSSTHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIATINSHFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIYQEKDWTLEKILQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFSALLCVISIGIILLASLHIITRPSQASEADSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGNYLRILAFL"
     gene            complement(18312..18509)
                     /locus_tag="VQM60_000744"
     CDS             complement(18312..18509)
                     /locus_tag="VQM60_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HET1080632.1"
                     /translation="MRSLSQEKDIYSFDEPTGNLDRNSTELFLNEVEKLVNEEKIVIV
                     VTHDKDVIARASKVINMDEFH"
BASE COUNT         6062 a         4193 c         3246 g         5887 t
ORIGIN      
        1 ctcacttttt caacgacaag aaagaggctg ataatctacc aacctcttgt ttttaaatac
       61 atttcaagtc caaactattc tttatagaaa aaagtcctat aaattcgagc aatagttaaa
      121 atacaaacaa atataattcc caaaatagat aacactattt tcacaaaatt catttcagaa
      181 cctattatat atacgtgaaa aaaaggggct attcctacac atatatacaa taaataactt
      241 acaaagttta tttcaccaat tcctgcaaaa aatgtttcat tttaagcaaa aatataacca
      301 tctaagctac cgtcagccca atcaacccca tatgcgattg ctccacctag tgaaaatcca
      361 gtaacagtta gtgcagtatt gactaaatgg ccagctgcac tacctacatg tttaattatt
      421 aaactacgta atgttctcgt aatagcccct ctattagctg agatagcttt ttttatcgca
      481 taagcagaat aaattgttgc accaccacta tagatggcca aatctacatc cagaactgtt
      541 gcaaaactac ctctactatt ccaccagtta caaccaacac cacccccatc aaggtacatc
      601 atctcttctt gctcaagagc aacataatta tttggtaata cgagttccat ttattttctc
      661 ctttttttat taagcatggt taggatggtt tcctaacaag tgatcgcagg ctttgtttag
      721 ttacctaaat tagacttcct tcctttatct tattttaaat atttaaaacc agttactgct
      781 tgaagcttaa cagtttactt aattcaaata acagctaatc actgattccc tgattttatc
      841 aaatatacta ataacaacaa ttgtgatacg atcatcatta taagaacaac atttaacgaa
      901 tcaaagtttt tccgagataa taataaagca acagcaagca gtattattac taaagattga
      961 ataactacta ttactcttaa cattttatcc tccactaaca actcaagctc ccttacatta
     1021 tatcgaccag ccccatggaa agattatagc tagactaatc aatgatataa tacatactag
     1081 aatacaaaat atttttttat tcatactttt tctcctatct ttattttcta cctacatagt
     1141 acctcttatt aagctcattt caaaattacg ttaacttttt tcacgaaaag gagaaaatta
     1201 tgcgttatga ttatggatag cgctttcaaa tttttaaact cctatcccat ccttttatct
     1261 atataataag tgaaaatata ataactgtca agtaactgaa gtgaatttta taaaaaaatt
     1321 acaagccaaa tttgtaaagt ttacactaag ccgctagggc aatcgtctat cagaatgtcc
     1381 gtttatttgt caataatccg agaaaatctt gcaacgctta gaagtctata aaaactatca
     1441 acatttatat gacttgcgaa tagcaatcct gctcaacctt tccacactct atctatacaa
     1501 tcaagataaa aacatgtgta agcaaatctg ctacacttta ctggaggacg ccaagaataa
     1561 gaaaagctac gataggcttg ctatctgcta tgttcgtatt gggatttgta gggataatgc
     1621 taaacttatc caaaaaggtt tctcactcct agagctgacc gaggaaactt ctatgctgtc
     1681 tcatctcaaa aaagaagtag agacccatta tcaaccaaag aaattataaa aaaagtcgag
     1741 ggatttcctc gactctttac atattatttg ttcgttagtt cttaatacca gccattgtta
     1801 aaccagaagt ttttagctct gccaagccac atagtgtgtt tactactatg tgtccatgtc
     1861 accatgaatc gccgaacgat ttaacgagaa agtatgactt ttacgtttat cccaactcaa
     1921 ttatgacatt tttttcaaaa gtcaatatat ctcacttttt caacgacaag aaagaggctg
     1981 ccaattgacc aacctcttat tctgaaccca tcactccatc actttttagc ttcattcgct
     2041 ttcttagcga ctgcaatctg gtattcgact tggtcattcc ccttacctgt acaaccatga
     2101 gcaattgtag tcgctcctgt ctgatgcgct atttcaacca attttttaga aataagaggg
     2161 cggctcaagg cagataccaa ggggtacttt tgttcatagt aggcatgtga ctggagagct
     2221 actagcacat aatctgtagc aaattcgtcc ttaacatcaa tgacataaga ttcgactgcc
     2281 ccaaccttaa gagccttatc atggatgaaa tctaagtctt tcccttcacc cacatccata
     2341 caaactgcaa caacatcata gtctttcttc agccaggtaa tagcaactga tgtgtctaaa
     2401 ccgccagaat aggccaaaat tactttttct ttactcatgt tcttttcctt ctttctattt
     2461 ttcaatggac gctttaaagg catcctcaat gagtttgtct aattccccag actccttcaa
     2521 cttttgaatg gttttatcga ctgcctcttt caattccttg ctatcttttt tcatggctac
     2581 cgcataggaa tcatcttgct ctttttcaaa attgaggtct gcgattgcta aatcaggatt
     2641 attttccaca aatcccttgg caacgggttc ttcaaaaata acggcatcca cttgccctga
     2701 ttttaaatct gtgattaaat tcccattttt aggcagagat acgagggaag aattttgtag
     2761 caaatctttc gccatcgtct cttgaatcga acctttctgc gctccgacct ttttctgcgc
     2821 caaatcgttg acagactgat aagtggctaa atcagatttt ttgacaatga gtttattttt
     2881 tgcagtatag tagggagttg aaaaatcaaa caccttgctc cgttcatctg tcttagaaac
     2941 acctgatatg gcaaggtcag cttttcctga ttgaacgcta gccagtacat tgtcaaaact
     3001 cataggagat agttccaatt cgacacctag ttctgttgcg atagccttgg ctaattcgat
     3061 atctgaaccc acaatctgat ttttcccatc aaccactttt tgatattcaa atggagcaaa
     3121 atctggattg agggctacaa ccaattttcc tttggacttg atggcttcaa ttccagccga
     3181 ttgattgtta ctacaagcaa atagtagggg gagcatcact aaaccaaaca tcgtcatcaa
     3241 caccttcttc atcttattca taataaagcc tcctttatgt ttattctttt ttattgtata
     3301 aataatactc ctatactcat cctttgtcaa gcctaaattg aaaaaatttt caatttgaaa
     3361 caaaaaaaac ctagaaatac tacataaaat gtcattccta ggcgtattta tgctattttt
     3421 ctctggaaaa catgagtatt cagccagtca aaaggagctg aatgaactca ttttccctcg
     3481 cccaattcaa tgacacgatg acattgttgg gctacataag catcgtgggt cacaataatg
     3541 actgttttcc cttctcgatt catctctaag agaaacttca agaccaaatc tctattttca
     3601 ggatccagcg aacctgtagg ttcatcggct aaaatcagct ggctgggttt taagatggct
     3661 ctagcaactg caattcgttg ttgttcgccc ccagacaact cggagaccct ttgatgcaaa
     3721 gtagctgaca aacctactct ctctaaaatc tcttccacct ttttgagctt gtctttctta
     3781 ggcaatttca catatttcag cgccagcatg agattgtact cgaccgtttc atcatcaatc
     3841 agggcaaaat tttgaaacag ataagagata tgttcacgga ttattgtttg cgacttagca
     3901 gaattaaccg ctagatttgt ctgaccaaaa atctcatacc gtccgctata atcaccatct
     3961 atcaaaccca ataaatttaa caaggtcgac ttcccactac cactcttacc aacaatagct
     4021 accaaatccc cctgatcaat cctgagagat aagttatcca aaatcacttt tcccccaatg
     4081 gttttggtaa tatttttcaa ctcaatcata agatgccccc tttcaataac tctactagac
     4141 ttcttttctc catcctagaa gctaagccta gcacaaatag tatatccaga catgtaaaac
     4201 ctgcaaacag tagaagtggt aagaacgcat gggcaaagaa aatcaagact agaagaggga
     4261 aactatagcc cagcaagagc agaacgagga gaggacggta gcgatcgacc agtttccacc
     4321 ccataaactt cttggtaatg atatccctgc gcttcaataa gaaagttgtt actagtaaga
     4381 agtaggaaat catcatgcta aggagaccaa acaaagcaaa gagtaggtta aaattccgaa
     4441 cagcatctcg ataagaatcc actttctctt gttgaatggc ttgaatagat gaaaatttta
     4501 aataatttcc atctgacaat ttctcaacta actctgtaat ctctttttga tgttgaaccg
     4561 tattttcaat tttaatcgga ttatttaagc cagttgttga cagggaggct ttctcatccc
     4621 acatcatatc agaatcattg accaagctaa taattggatt ggagagattt tcctttcgct
     4681 tatcactata tgggaaaaat gaccaatctc cttcataata ggcaatctcg acatccatct
     4741 cctctatcgt tcgtttttgc tgctcttcat acttcatcga atgaaaggca attaacttcc
     4801 ccaagagctg atttttatct tcttcacctt tcgtacttgc tggcatcaaa ataacttttt
     4861 taataccggt atctggtagc ttgaatccct tgctctttag aaaattgcga ttggcatagt
     4921 aaacatccac cgtatctgtt aactgatatt gctgaatctg ttctgattgg acaaaatttt
     4981 ttacaggaag actgctactc tgcacatagc ccgcctgcgt tttttctacc aaatcctgat
     5041 aaaatcgata gaaataatcc aatgccttcc ctgagcctgc caactgctct tgccacaagt
     5101 tatcattgag ttggaaagtt tctaaggtca gataatttcc ttggctgatc cactgtttct
     5161 ggtaatccaa ctctttgttt tcttgctcca aactttttcc taccccaact agcaagactg
     5221 tcagtaaaat ggtagttcca attttcatca aataattaaa gagtagacca aatttgaaag
     5281 atgaaaaacc tttcagcaga gagctgattg tcattttttg gattaagagg taagtcaacc
     5341 aactgataaa gagataaagc tgcaacagca aaaaatgaga caaccacagc ataggaaaca
     5401 aatcttttgg cttataatca agcaagaaaa acacgcctag attgatcaca agagccccac
     5461 ctaggaggag gtaaaggttg ccttttacaa catcagctaa aacagcccta tcttgaaaac
     5521 caagtaattt ttgtacccca actcgtttca tctccatcat cggttgatac actgtcacta
     5581 acacaagaag caaaatagcc aagacaaaaa caatggcaga taaaagcaaa tctcgattta
     5641 tgacttccac tgcacttttg taggtcggct ctagcaaggt agcctggtct atcttgaaaa
     5701 aatcgctcca tttctgtaca atcctatcct tgtccatctc ttgtgtagaa gttatcgtat
     5761 agcgaccatt taaactacga gatgtatcct tgatataggt ttgaaaagtc ataagctgaa
     5821 taggtttggc ttttagaaag gtcggaatcg taccaagttt attggaaatt tctttattac
     5881 tatagactcc ttcaccatct gtggtaaaat caagagaaga aatcccaaac tcttggtagg
     5941 ggaaggtatc tttatcaaaa acaccagact tgaccacctc atcaccactg tctgttttga
     6001 tgatggagac tttatactcc tttgatacat cctcaaaaaa tcgaagaaca gacgctgcag
     6061 gttcgttaat atctttcaaa tacaaatcca aagaatctac agtctttccc atttcttgaa
     6121 tccgaaccac ttctcgtgta tagtttacat taaaaacaaa aaaagtggta cacagaagca
     6181 ggagtaacat acaaagatga ctgatttttt tcatagagat acctcccgtg aagaaactcc
     6241 ctctccaaaa ggggagggag aattttaatt aaaatccata gaaatattcc attcccgttg
     6301 gtgggaattt gaagattgaa gcccgagccc atgcctcagc ttctgcacga tccttagaga
     6361 atttatttcc atctttaaca gttgctgtat gagagcgagt agaatgtaaa taatcagaat
     6421 aaccaaaatt tcctgaccat cctactccat agttccattg accaccatca acccatactg
     6481 ccaaaacact tgtagaagca agtatactac ataccattaa agatgcaaca ccaattttct
     6541 catgatttat ttctcctatt atcgagagct attccgtaga aacagctata gtaatttgtc
     6601 aacaagtatc gtagtccatt ggactcacct tccttctatc tcatttcatt tttaaacaac
     6661 gaacttactc ttaactagaa gaatagttct cttctgtatc aggtctttga acaaccagag
     6721 tagtattaat cacactctaa ctattactca agaatcagcc caataaacaa tatggatagc
     6781 gctttcaaat ttttaaactc ttatcccatc cttttatcta tataataagt gaaaatataa
     6841 taactgtcaa gtaatcgaag tgaattttat aaaaaaaatt acaagccaaa tttgcaaagt
     6901 ttacaataag ccgctagggc aatcgtctat cagaatgtcc gtttatttgt caataatccg
     6961 agaaaatctt gcaacgctta gaagtctata aaaactatca acatttatat gacttgcgaa
     7021 tagcaatcct gctcaacctt tccacactct atctatacaa tcaagataaa aacatgtgta
     7081 agcaaatctg ctacacttta ctagaggacg ccaagaataa gaaaagctac gataggctgg
     7141 ttatctgcta tgtccgtatt gggatttgta cagacgattc taaacttatc caaaaagggt
     7201 tctcccttct ggagctaacc gaggaaactt ctatgctgtc tcatctcaaa aaagaagtag
     7261 agatctatta ccaagcgaag gaaagataaa aaagccgagg gatttcctcg actctttaca
     7321 tattatttgt tcgttagttc ttaataccag ccattgttaa gccagaagtt tttagcagca
     7381 gtccatgaac cgtaacgtcc tgcaacgtag gcatctgcta cacgttcttg gttttcagct
     7441 gagtagtcac cgttcaggta tgaatctgtt aattggtaac gtccgatata acgtccattt
     7501 gtagctgtat agctaccacc tgattctttt tgagcgatcc attctttggc ttctgcttca
     7561 gatccgctta cagttgaaac aactgtttca cttactactg gagtttctgc tactggagct
     7621 gaaacagttt catcttgagc ggctggtgcc gcataagtag ctggcgctgg tgttgcaaca
     7681 ggcgctacag ggccatcgat aaccaactct tgaccaacat aaatcaaatg aatgttatca
     7741 atgtggttgt tttctgccaa tttttcaact gttgtgttgt gagtttcagc gatttctgaa
     7801 agtgtatcac cttctttaac agtgtaagtt gatgattctt gagcagatac aaatgatgga
     7861 gcaaatactg caaacaaggc agctactcct gcaagagttg ctttaatctt tttagttatt
     7921 gatttcatat tcgaaaattc tccttctttc tatagttcta tcataccctt taaatattac
     7981 cgtttcttga cacttttatg tagaaatatt acaaaattat tttttatttc cctaaataag
     8041 gtgttttttg tcataaaaga tactattttt atgctatttg aggtattaga aaggttttca
     8101 tccacaatta aagccaagcc cctcattctt tgatataata gagataagaa tttttataag
     8161 gggaaactga tgaaatcact tcgttttcaa tctgtctttg atatcatcgg accagttatg
     8221 attggcccat ctagtagcca taccgctggt gctgttcgta ttgggaagat tgtctcttcc
     8281 atttttgatg atactccgac agaagttgaa ttccaacttt ttaactcatt tgccaagacc
     8341 tatcgtggtc acggaacaga cctagccctt gttgcaggta ttttgggaat ggatacagat
     8401 gatcctgaaa ttccaaatag tctggaaatt gcccacaagc gtggtatcaa gattgtctgg
     8461 accattcaga aagacagcaa tgcccctcac cctaacacca ctaaaattac tgttaaaaat
     8521 gcccacaaga ccatcagcgt gactggtatt tctatcggtg gaggaaatat tcaggtaacc
     8581 gaactcaatg gttttgccgt ctctctcaat atgaatacac cgactatcat catcgttcat
     8641 caagatattc caggtatgat tgcccttgtt acagaggcgc tttcccgcta tggtatcaat
     8701 atcgcccaga tgaatgtcac tcgtgaaaaa gctggtgaaa aagccattat gattatcgaa
     8761 gttgacagtc gcaactgtga tgaggctatc gaagaaattc gaaaaatccc tcatctccac
     8821 aatgtcaatt tctttaaata ggaggaagca tgttttattc tatcaaagaa ttggtcgagc
     8881 aagcagatct agactttcaa ggaaatgtcg cagaactcat gattacaaca gagtttgaat
     8941 tgaccggtcg cgaacgtgaa gaagtcttcc ttctcatgga acgcaatctg gaagttatga
     9001 aagcctctgt ccaacttggc ctcaatgaaa ataaatctcg tagtggcctg acaggtggag
     9061 atgctgccaa attggatcac tacatcgaaa acggaaaaac tctgtcagat tatacgattc
     9121 tctcggctgc ccgaaatgcc atcgcagtca atgaacacaa tgccaaaatg ggcttggtct
     9181 gtgccactcc aaccgctgga agtgctggct gtctgccatc cgttctcact gctgctattg
     9241 aaaaattaga cctcagccac gagcaacaat tggatttcct ctttgctgct ggtgcctttg
     9301 gactagtcat cgcaaacaat gcctccatct caggtgctga gggtgggtgt caagctgaag
     9361 ttggttcagc ctctgctatg agtgctgccg ccttgactct ggctgcaggt ggaacacctt
     9421 atcaggccag tcaagctatt gcctttgtca ttaaaaatat gctaggcctc atctgtgacc
     9481 ctgttgcagg tttggtcgaa gttccctgtg tcaaacgtaa tgccatggga gctagctttg
     9541 ctttcatcgc agcagacatg gccttggcag gtatcgaatc taaaatccct gtggatgaag
     9601 tgatcgatgc catgtaccaa gtaggagcaa gcatgccaac tgcctttcgt gaaacagctg
     9661 aaggtggact cgctaccacc cctactggtc gtcgcctcca aaaagaaatt ttcggagaat
     9721 aagcttatca aataggagaa accatgacct ctatcacagc gatttttttc gatttggatg
     9781 gaaccctcgt tgatagttct atcgggattc acaatgcctt tacctatacc tttaaagagc
     9841 tgggggtgcc tagccctgat gccaaaacta ttcgtggttt tatgggacca cctctcgaaa
     9901 gtagttttgc gacctgcctg tccaaagacc aaatttctga agccgtgcag atatatcgtt
     9961 cttactataa ggcaaaaggc atctatgaag ctcaactctt tcctcagatt gtagaattac
    10021 ttcaagaact atccaagaat taccctctct acatcaccac tacaaagaat acacctaccg
    10081 ctcaagacat gacaaaaaac ttgggaatcc atcatttctt tgatggcatt tatggttaca
    10141 gccctgaagc accccataag gcagatgtca ttcaccaagc cttgcagata catcaactag
    10201 caccagaaca agccatcatc atcggagata ccaagtttga tatgctggga gctcgagaaa
    10261 caggtattca gaaattggcc atcacttggg gatttggaga gcaagcagat ctactaaact
    10321 atcaacctga ttatatcgct cacaaaccct tagaagtttt ggagtatttt caataacata
    10381 gactaggcaa caactccatt tcagaagaaa atgaagtaat actcaatgaa aatcaaagag
    10441 caaaccagga aagtaaccgc aggctgctca aaacactgtt ttaaggttgt agatggaact
    10501 gacgaagtca gctcaaaaca ctgttttgag gtggcagata gaactgacga agtcagctca
    10561 aaacactgtt ttgaggtggc agatagaact gacgaagtca gtaaccatat ctacggcaag
    10621 gcgacgttga cgtggtttga agagattttc gaagagtata agtctctaca taataaaacg
    10681 catagtacca aggttaacaa acttgatact atgcgttttt cttttttatt caatgtgggt
    10741 tgtttgatta gcaaaggcga taatagcttg cttcaactca tctccactga gagtgcggaa
    10801 ctctccaatc ttttgattga tggattctaa aggcatgaca ttaaccttac caacgaaaat
    10861 cttatccata acttgattgt caacgagttc ggttgatacc aagagttctt cgtagagttt
    10921 ttcacctggg cggattccaa cttcaacgat tggaatttca ctttcagtgt ggccacttag
    10981 aagcaccatc ttcttggcca agtcataaat cttgactggt ttgcccatat caaggataaa
    11041 gacttcccca tctttggcat aagcaccagc atggataacc agacggctag cttctggaat
    11101 ggtcataaag taacgggtca tgcggaagtc tgtcaccgtt acaggcccac cttcagcaat
    11161 ctgacgttca aagactggaa tgacactacc acggctacca agaacattcc caaaacgaac
    11221 tgcacagtag gttgattggc tacgttggtt aaagccagtg acaatcaact ccgccacgcg
    11281 cttggttgct cccataacat ttggtggatt gactgcctta tctgtcgaaa tcataaccat
    11341 cttagacact ttagcttcat caacagcctt agcaacattg taagttccac ggatattgtt
    11401 tttgaaggct tcttttggat tgcgctccat cataggaaca tgcttgtggg ctgccgcatg
    11461 ataaacaata gcaggtttgt actgctcaaa gacttgcaac aaacgatcat agtcttgaat
    11521 gtccgcaatc acaggtacat aatcaatccc ttggaactta cgaatcaatt catgataaac
    11581 aaggtagatt gagttttccc catgaccgag caagacaatg cgttcaggat tgaagcgact
    11641 aacttgacga cagatttcag aaccgattga acctccagct cctgtgacta agatggtctt
    11701 acctgtcagt tctgcaccca gacgcgattc gtcaagacgg atttcctgac gacccaaaag
    11761 gtccgtaata tcaatttttt ggaagccagt acctgcttgg tgaaggccct gaacaacagt
    11821 ttcaacctta ggcatcttgt aacatttgac acccagctta ttacacatct gcaagatacg
    11881 ctcatattct gacggatcca gcgacggaat cgcaacgatg acacgctcga tttgatggcg
    11941 tttggctaat tcaggcagat tgtcataaga gcccaaaaca ggaataccac caagtttttg
    12001 accctttttc ttagaatcct tatccaaaat accgaccagt tctaattcac tggttggatg
    12061 ttggtaacta tccataaaga gagccccacc atcaccggca ccaatcaaga aggtccgacg
    12121 gtgttctcca tcaccactac cttttttgcg tctggagtag attaactgcc aagtaatccg
    12181 tggcaataaa atcaagaagg tactcaacaa gataaagaga atgataaaac ggatggagaa
    12241 gagtggcaag aaggcataac agatactata tgacaagaca ctgctagcag tcacaccaaa
    12301 aaagattttc atgaaatccg taatcttgct gtaacgacta atgctcgcgt tcaaccccca
    12361 aaaaccaatc atcaattgat agaacaggaa ggccaaactc gtatagataa tgtagtcaac
    12421 aggtgctgga ttaatcagcc cataaaataa aatataagat acaatgatgg aaaccaccat
    12481 actgaaaata tcaaatattc cccagaaaac ctgcttttgt tgtttattta aaatttccac
    12541 cagatcaatc acataatctg ttagtttttt attcatagaa atctactcct ccttaaaaga
    12601 gttttactat attgttatta tagcatattt ttatttattt ggcttatttt atccaaataa
    12661 attattgttc tcttgtactc cttcttccat ttaaatcctc taattaaacc tacaatcgtc
    12721 ccaaagccat aagcaaagtg aatggaaaat aaaagaaagg gcatcacaat tagaaatcca
    12781 tttttatgtt ttaataaagt cagcaaagtg agtagtgaca aaagtagaaa ataggcacct
    12841 aatagtaaag ttatgaatac gaatgtgatc ggtaacaatg ctagactaaa cacaagactc
    12901 aaaacaaata aacaaggaac atagtgaaat aatgataaac acttaaactg aacatgactt
    12961 gtcaagccaa tccacaaacc atttaaatac ttttgatgca gcattttctt gaatgttggt
    13021 cgaatatact gataagatag aatacttggg ctatagcgga ttttataacc atattctcga
    13081 attctataat gaatatcatt atcttcagtt cggccaagtt gctcatttac taaaccaacc
    13141 ttctggaaaa cctctcgttt atacattcca tgaaaaatag aagaaacata tctatcctca
    13201 gaactatttc gataattggc aatgctactg ccaaacatat tttcctcaac aagatgcaag
    13261 gtctctgccc attttccttt tccttcgaca atcgtcggtc taggcccccc acagacaaat
    13321 tcaccttgtt gaataatagc cacattgttc attacaaaag tctcagtaac ttttgaatga
    13381 gcatcaattt ttaaaataag gtcccctaca gaatgtttaa ctcccaggtt aaaaccacta
    13441 gcttgatttt tcttaggatt gttatacaat ctaattgagt taaactctgt atcttccttt
    13501 ataaattgct gaatgatagc tgtggtccca tctgtggaca tagcatttat aaatagaatt
    13561 tcaatattct ctttaggata ggtttgattt tttaagtctt caattagacc aggcagatat
    13621 ttttcttcat tgtatgcact gattacaatg gatacgagaa gactttcaac attttgagtc
    13681 actactactc tcctgaattt ttccttccac cattctatca taattttcaa gactttccca
    13741 tttttatctt gaaagccaga aaccttactt gacattatag tgaaaaagcc ttaaaataag
    13801 ctgttattaa aatcagctag aagaggtatt atatgaaaaa gcaatcactc ttttttgttc
    13861 caggtattat cctgattggt gtttccttgc gaactccttt tactgtttta cccattattt
    13921 tgggaaatat ttcgcaaggt ctggaggtag aagttagttc gcttggtgtc ttgaccagcc
    13981 tgcctctcct tatgtttacc ctcttttcac cattttctac ccaactggct cagaaaatcg
    14041 gcttggagca tctcttcacc tacagcctct tcttcttgac catcggctca cttattcgac
    14101 taatcaatct gcccctgctc tatctaggaa ccttgatggt tggggcaagt gtcgcagtca
    14161 tcaatgtcct gcttcctagt cttatccaag ccaatcaacc aaagaaaatt ggttttctga
    14221 ccaccttata tgtaacgtct atggggattg caacggctct ggcttcctat ctagctgtgc
    14281 ccattacaca agccagttct tggaaaggac ttatcctcct cctcacgtta ctctgtctag
    14341 caactttttt ggtctggctc ccaaatcacc gctataatca tagactagct ccacaaacca
    14401 aacaaaaaag tcaaataaag gtcatgcgta ataaacaggt ttgggcaatt attatctttt
    14461 caggttttca atccttgatc ttttacaccg tcatgacctg gttacctacc atgtctatcc
    14521 atgcaggtct atccagtcac gaagctggct tgctgacttc tatcttatct ctgattagca
    14581 ttcctttttc aatgaccatc ccaagcctga caaccagttt atctactcgc aaccgtcagc
    14641 tcatgctcac tctggtttca ctagctggtg tggtcggcat ttccatgctc tttttcccaa
    14701 tcaataattt catttactgg cttgccatcc atctcctcat cggaaccgca accagcgccc
    14761 tcttccctta tctcatggtc aacttttcac tcaagacaag cgcccctgaa aagacagccc
    14821 aattgtccgg cctatctcaa acaggaggct atatcctagc agcctttggg ccaaccctct
    14881 ttggttacag ttttgacctg ttccactctt gggtaccatc tgtagctgcc ctcttgctca
    14941 tcgatatcct gatgactgtg gccctcttta cagtggacag agcggataag atcctttaaa
    15001 cttctagttt tagctagaag ttttttatat ataaaaattt tacacatttc tcttgacagg
    15061 gctttctttt agatgtacaa tgtatgtgta gaaaaattat atataaaaat cctacacatt
    15121 agaaaaggag gtttcccatg tactttccaa catcctctgc cttgattgaa tttctcatct
    15181 tggccgtact ggaaaaaggg gattcttatg gttatgagat tagccaaacc attaagctga
    15241 tcgctaatat caaagaatcc acactctatc ccattctcaa aaaattggaa ggcaatagct
    15301 ttctgacaac ctattctaga gagttccaag gtcgcatgcg caaatactac tccttgacaa
    15361 atggtggtat agaacagctc ttgaccctaa aagatgaatg ggcactctat acagacacca
    15421 tcaatggcat catagaaggg agtatccgcc atgacaagaa ctgaatacct gactcagcta
    15481 gaactctatc tcaaaaaact gcctgaagct gaccaaattg aagccatgga ctatttcagg
    15541 gaactctttg atgatgctgg agtcgaagga gaggaagaac tcatcgctag cttgggaact
    15601 cccaaagaag cagcccacga agttctctct aatcttcttg ataaaaaaat caatgaagca
    15661 cctgctcaaa aaaataaccg acaaatttta catgtcgcct tattagccct ccttgcagca
    15721 cccatcggta ttcctctggg aatcgccatc ctcgtgtccc tgttcgcaat ccttgtggca
    15781 gccttgactg tcatcttggc tttctttgcg gtttccatac tgggtatcat cggtggattc
    15841 ctatttttag ttgaaagttt cactgtccta gcccaagcca aatcagcctt tatcttgatt
    15901 ttcggttctg gtttactggc tatcggtgct tcttcactag tcttactagg tatttcctat
    15961 gtagcccgct tcttcggtct actcattgtt cgcttggtgc aatttgttct taaaaaagga
    16021 aagagaggtg accagcatgc gtaaattgac aaaaggattt ctcatttttg gtgtcgtgac
    16081 taccgttatc ggctttatcc tgctctttgt aggtatccaa tctgacggga ttaagagcct
    16141 acttgccatg tccaaggagc ctgtctatga tagtcggatg gaagagttaa cctttggcaa
    16201 ggaagtcgaa aacttagaga tcactcttca ccagcacgcg ctgaccatca cagactcttt
    16261 cgatgatcaa atccacattt cttaccatcc atcactttct gctcaccatg atcttatcac
    16321 caatcagaac gatagaactc tgagtctcac tgataagaaa ctgtctgaaa gtccatttat
    16381 ctcttctgga attgatggga ttctccatat tgtaagtagt tcatctaatc gttttaatga
    16441 tgtcatcatc ggactaccga aagggagaac tctaaaaggg atcaacatct cagccaatcg
    16501 cggacaaacc accatcataa atgctagcct tgaaaatgcg accctcaata caaacggcta
    16561 tatcctccga attgaaggaa gtcgtatcaa aaacagtaaa ctcacaacgc ccaatatcgt
    16621 taatatcttt gatacagatc ttacagatag tcagctagag tcaacagaac atcacttcca
    16681 cgctgaaaat atccaagtcc atggtaaggt tgaactgact gccaaagatt atctccgaat
    16741 catcctagac cagaaagaga gccaacgaat taattgggat gtctcaagta actatgggtc
    16801 tatcttccaa ttcacaagag aagagcctga atcaagaggt acggaattaa gcaaccctta
    16861 caaaactgaa aaaaccgatg tcaaggatca actcattgcg agatctgatg atgatattga
    16921 gctaatatcc acaccaagca gacgttgaca aaaacgctta catctgctac tatggaaaca
    16981 tatttatcta actaaaaagg aggttctacc atgaaacaag aatggtttga aagtaatgat
    17041 tttgtaaaaa caacaagcaa gaacaagcct gaagagcaag ctcaagaggt tgcagacaag
    17101 gctgaagaaa ggatagccga tctcgataca ccaattgaaa aaaatactca agtagaggag
    17161 gaagtctctc aggctgaagt cgaactggaa agccagcaag aagagaaaat ggaaactcct
    17221 gaagacagtg aagcgagaac agaaatagaa gaaaagaagg catctaattc tactgaagaa
    17281 gagccagacc tttctaaaga aacagaaaaa gtcactatag ctgaagagag ccaagaagct
    17341 cttcctcagc aaaaagcaac cacgaaagag ccccttctta tcagtaaatc tttagaaagt
    17401 ccttatatcc ccgaccaagc tccaaaatct agggataaat ggaaagagca agtgcttgat
    17461 ttttggtctt ggctagtgga ggctctcaaa tctcctacaa gcaagcttga aacaagtagc
    17521 acacacagct acacagcctt tctcttgctc attctgtttt ctgcatcttc ctttttcttt
    17581 agtatctacc acatcaaaca tgcttactat ggacatatag caactataaa cagccacttc
    17641 cctgagcaac tagcaccttt aactctcttt tctatcatct ctatcctagt agcgacaaca
    17701 ctcttcttct tttcattcct cttgggtagt ttcgtcgtga gacgatttat ctaccaagaa
    17761 aaggactgga cgctggaaaa gattctccaa caatatagtc aactcttggc aattccaatc
    17821 ttcctcactg ccattgctag tttctttgct ttctttgaca gcctacgatt ttcagccctt
    17881 ttatgtgtga ttagcattgg aatcattctg cttgctagtc tccatatcat taccagacca
    17941 agtcaagcaa gtgaagccga ctccttctat cagttattct tgtctgtcct tgtgaacgga
    18001 gttattatcc tcctcttctt tgtagctgaa gtggcactga ttggaaatta tcttcgtatc
    18061 ttggcctttc tttaaacttc aatcctgtca tccttggcag gattttttct atgtcaaaaa
    18121 agcaagtcga ttgacctgct tctgctttac ttttcttgtg ctaatgtttt aaaatcttgt
    18181 cctaggatgg gttttattcc tagagttcaa agtatgatag cgttgctata aagttcgtta
    18241 ttccaaatat tatgcaaact agttatatgt aaaattaaga ttagaaacca ccaatctcca
    18301 tttattaact tttaatgaaa ttcgtccata ttaataactt tacttgctct agcaataaca
    18361 tccttgtcat gagtcacgac aataacaatc ttttcctcgt tcactaattt ctcaacctcg
    18421 tttagaaata attcagttga gtttctgtcc aaatttcctg taggctcatc gaatgaatag
    18481 atatcctttt cttgcgataa actcctcatg aatagaattt tttgtttctg accttacgaa
    18541 atattggtgg ctttcccatc aatctgatga ctcagagcta tattgagatt caaactatca
    18601 agattatttg attcatagtt ttccttatca aatagggtga aattaatatt ttttgtcatg
    18661 cttcatcacc tccagatata gtattaaaat tttagatcag ttgactatcc gtgtcgaatc
    18721 tttcatagaa actcttgtta ttatagaaat aaataacaaa ttattagtac atgaataata
    18781 aatctgatac aatggtgact ataattgatt ttatttccac taatctttct atcgtagagc
    18841 aagctcactg aaagtaatat taaaaagatt gatattaata atttcaaata gttaattgaa
    18901 taaaataagt taaacaatgt cgaagtacat aaacataagt ttataagtat tgatacaaca
    18961 gatactccaa gctttttttt atatccaaaa taagctgaaa acattaaaat aatagaatag
    19021 gcacaaataa tataattcat ttatttttcc tccaaagtca aataataaat ctaatactac
    19081 tatataaaat atacaataat ccaattatag aaataataaa aaataaatcc caaatatacg
    19141 tttttaggtt atttctcttt actacttctt gcaattttag attatttctt attccaatac
    19201 aataaaaaag ggttttaaaa aataatgatc gtattttata aattccaaaa aattcttgat
    19261 aaagtatata accatctgtc tttaataaga atggataagc attaataaat gcaaaaatca
    19321 atgtcccaac agcatagatg ctcgtataat atgctgctgt ataaagtttt aaatactgta
    19381 ataagtaa
//
LOCUS       DAVAAL010000025        19214 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVAAL010000025 DAVAAL010000000
VERSION     DAVAAL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19214
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.026"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..1391)
                     /locus_tag="VQM60_000745"
     CDS             complement(<1..1391)
                     /locus_tag="VQM60_000745"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET1080633.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKILAFTGTKGKTTAAYFAYNILSQRYPTALLSTMNTTLDGTTFFKSSFS
                     TPENIDLFDMMAQAVKNGRSHLVMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQII"
     gene            1497..3119
                     /locus_tag="VQM60_000746"
     CDS             1497..3119
                     /locus_tag="VQM60_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET1080634.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            3320..5281
                     /locus_tag="VQM60_000747"
     CDS             3320..5281
                     /locus_tag="VQM60_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET1080635.1"
                     /translation="MKKSKSKYLTLAGFVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRATTNDIVTNLVDGLMENDQYGNYVPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGI
                     KALDDQTVQYTLARPEPYWNSKTTSTILFPVNADFLKSKGDDFGKVDPANILYSGPFL
                     MKAFVSKSVIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQDALVRNFTDGAYSYARLYP
                     NSSSFEGIKEKYKDDIIYSMQDATSYYWNFNLDRQAYKFTSKTTDIEKKSTQEAVLNK
                     NFRQAINFAYDRTSYGAQTQGEDGATKILRNLVVPPTFVSIKGKDFGEVVASKMVNNY
                     GKEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDATVDQASKKGIQE
                     VSSMKQSIEAALGTENVVIDIQQLSTEDYDNSSYLAQTAIQKDYDLYNGGWSPDYLDP
                     STYLDIFSVKNGGVLQNLGLDPGEANDKAKAVGLDTYTQMLEEANKEQDPAKRYEKYA
                     DIQAWLIDSSLAIPNVSLGGTPSLRKTVPFSAPYSLAGNKGVESYKYLKVQDKIVTTD
                     EYAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            5453..5686
                     /locus_tag="VQM60_000748"
     CDS             5453..5686
                     /locus_tag="VQM60_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HET1080636.1"
                     /translation="MKLKLLRVDTKVIMWSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            5692..7134
                     /locus_tag="VQM60_000749"
     CDS             5692..7134
                     /locus_tag="VQM60_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080637.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDMSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSFETPFALLFI
                     FATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTIYSSSKGSSVA
                     LENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIART
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            7292..8386
                     /locus_tag="VQM60_000750"
     CDS             7292..8386
                     /locus_tag="VQM60_000750"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET1080638.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            8394..9560
                     /locus_tag="VQM60_000751"
     CDS             8394..9560
                     /locus_tag="VQM60_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET1080639.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVMDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            9653..12751
                     /locus_tag="VQM60_000752"
     CDS             9653..12751
                     /locus_tag="VQM60_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET1080640.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            12799..13416
                     /locus_tag="VQM60_000753"
     CDS             12799..13416
                     /locus_tag="VQM60_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET1080641.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            13427..14761
                     /locus_tag="VQM60_000754"
     CDS             13427..14761
                     /locus_tag="VQM60_000754"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET1080642.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            14771..15235
                     /locus_tag="VQM60_000755"
     CDS             14771..15235
                     /locus_tag="VQM60_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1080643.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            15232..15726
                     /locus_tag="VQM60_000756"
     CDS             15232..15726
                     /locus_tag="VQM60_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1080644.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            15807..17462
                     /gene="mltG"
                     /locus_tag="VQM60_000757"
     CDS             15807..17462
                     /gene="mltG"
                     /locus_tag="VQM60_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET1080645.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADESQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATEIVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDKIAQAVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            17533..18015
                     /gene="greA"
                     /locus_tag="VQM60_000758"
     CDS             17533..18015
                     /gene="greA"
                     /locus_tag="VQM60_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET1080646.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            18343..18741
                     /locus_tag="VQM60_000759"
     CDS             18343..18741
                     /locus_tag="VQM60_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1080647.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT         5792 a         3540 c         4266 g         5616 t
ORIGIN      
        1 tggatgattt ggtaacaatc ggctccctta cccgcaataa taactgcatc taattcgtga
       61 tttgtgatag ccattgccgc cttgatggct tcttggcgat ccgcaatctt ttcaacagga
      121 tgattgatgt agctactaat ttcatctgca atggccattg ggtcttcata gttagggtca
      181 tcagcagtca gaaagacttg aatctcaggg tgttgattga ggaggaggcc aaagtcctta
      241 cgacgacttt ctcccttgtt tcctgttgat cccagaacca gagcaatctt tccggtttga
      301 tgagtttcaa ccacattgat gagttttttc agactatccc cattgtgggc atagtcgatg
      361 aagaccttgg ctccattttt ctgagtgagg acttccatac gaccaggaac gcgggttgca
      421 gcgatgcctt ttttgatgtc ctcaagactt gctccgagac ggagacaagc aagtccagca
      481 gcaactgcat tttcttggtt gaagttgcca atgagttgga tatcataatc tccagcgagt
      541 ttacccgtag ctgaaaagct aaaggctttg gaattctcga tttggttatc aaattggcta
      601 ccatagaaat catggtcttg atcttcaacc tgttctttca agactgagaa gtggtccatg
      661 tcactgttaa tgatgactgc tcggctattt tccatcaaga gacgcttgtg gtagaaatag
      721 tcttcaaagc tagggtgttc aatcgggccg atatggtcag gagtgatgtt aagaaagact
      781 cctacatcaa aggtcagtcc atagactcga tggactagat aggcttggct agagacttcc
      841 attacgagat ggcttcttcc attcttaaca gcttgagcca tcatgtcaaa gaggtcgata
      901 ttttcaggtg ttgagaatga agatttaaag aaagttgtgc catccaaagt tgtattcatg
      961 gttgataaga gggcggttgg gtaacgttga gataggatgt tgtaagcaaa gtaagctgct
     1021 gttgtcttac ccttagttcc tgtgaaggcg agaattttga gtttctcttg tggattacca
     1081 tagaactcca tggcaatcaa actcatggct ttctttatat cgttcacaat gatgacaggg
     1141 ataccgactt cgtagtcctt ttcagctaca taccaagcta aaccttgtgt tatagcagaa
     1201 agaaggtatt cttttttaaa ggcagcacct tttgcaaaaa aaagagtgtc ttctgttact
     1261 tttcggctgt cgtagctgat gctatcaaaa acaactttgc tgtagttgta gtggtaatga
     1321 ccttggtcaa taatttcgcg aaaaaggcca tctttcttta aaatatctaa tacagtttca
     1381 atttttatca tactttctat tgtaaaccga aagtcgcaaa tttacaagta acaaggaaaa
     1441 gtttataatg gaagataagg agtttttcct agttatcaaa attgaatgag gaatctatgt
     1501 cgcacgaaaa caatcaccag caggcccaga tgttacgggg gactgcttgg ctaacggcta
     1561 gtaactttat cagtcgccta ctcggggctg tttacattat cccttggtac atctggatgg
     1621 gggcttatgc agctaaggca aatggtctct ttaccatggg ttacaatatc tatgcttggt
     1681 tcttgttggt ttcaacagcg gggattccag ttgcggtggc caagcaagtt gccaagtata
     1741 ataccatgcg agaagaagag catagctttg ccctgattcg gagcttctta ggctttatga
     1801 caggactagg cctggttttt gctttagtct tgtatgtctt tgctccttgg ctagcagact
     1861 tgtctggcgt gggcaaagac ttgatcccaa tcatgcaaag cttggcttgg ggagtcttga
     1921 ttttcccgtc tatgagtgtt atccgaggat ttttccaagg gatgaataac ctcaaacctt
     1981 atgccatgag ccaaattgct gagcaggtca ttcgtgttat ctggatgctc ctagcaacct
     2041 ttatcattat gaagctcggt tcaggagatt atctagcagc cgttacccaa tcaacctttg
     2101 ctgcctttgt cggtatggta gccagttttg cagtcttgat ttatttcctt gcccaagaag
     2161 gttcactcaa aagagtcttt gaaacaggag ataagattaa cagtaagcgt ctcttggttg
     2221 ataccattaa ggaagccatt ccttttatcc tgacagggtc tgccatccag ctcttccaga
     2281 ttttggatca gctgaccttt atcaatagta tgagctggtt taccaactac agcaatgagg
     2341 acttggttgt catgttttct tatttctcag ccaatcctaa taaaatcacg atgattttga
     2401 tttctgtagg ggtttcgatt gggagtgttg gtttgccact tttgacggaa aactatgtca
     2461 agggggactt gaaagcggct tctcgtctcg ttcaggacag tctcacccta ctctttatgt
     2521 tcttgctacc agcaacggtt ggagtggtta tggtaggaga acctctttat acggtcttct
     2581 atggtaagcc agatagtttg gctctgggct tatttgtctt tgcagttttg cagtctatta
     2641 ttttaggctt gtacatggtc ttgtctccaa tgcttcaggc catgttccgc aaccgcaagg
     2701 ccgttctcta ttttatctat ggttctattg ccaagctagt cttgcaacta cctaccatcg
     2761 ccctcttcca cagttatggt cctttgattt caacaaccat tgctctcatc attcctaacg
     2821 tcttgatgta tcgggatatt tgtaaagtaa ctggtgtcaa gcgcaaggtg attttgaagc
     2881 gaaccatttt aatcagtttg ctgaccctag tcatgtttct gttaatagga accatccagt
     2941 ggctgttagg atttttcttc caaccaagtg gacgtttgtg gagcttcttt tatgtagctc
     3001 ttgtcggtgc catggggggt ggactttata tggttatgag tctgcgtacc tatttattag
     3061 ataaggtaat aggaaaagcc caagcagatc gcctgcgagc aaaatttaag ctttcgtaag
     3121 gtagcattta taaataaaag gaataagttg aacaatcttt taggagaact tgaaacttat
     3181 tcctttttta gcgtcaaaat agctaaaatt atctttcaaa aatagaaaat gagttttttg
     3241 attttttgga aaaaacaatt gactaaacaa accaagagtt gtataataaa acaaatattt
     3301 aaagcaggag gttctggaaa tgaaaaagtc taagagcaaa tatctaacct tggcaggttt
     3361 tgtcctgggt acaggagttt tattgagcgc gtgtggaaat tctagcactg catcaaaaac
     3421 ctataactat gtttattcaa gtgatccatc tagtttgaac tatctagcag aaaaccgcgc
     3481 aacaaccaat gatattgtga ccaatctggt agatggtctc atggaaaatg accaatatgg
     3541 gaactatgtg ccatccttgg cagaagattg gactgtttct caggatggtt tgacctatac
     3601 ctacaaactt cgtaaggatg ccaagtggtt tacttctgag ggagaagaat atgcacctgt
     3661 aactgcccag gattttgtga caggattgca atatgcagct gataaaaaat cagaagcctt
     3721 gtatctagtg caggactctg ttgcaggttt ggatgactat atcactggta aaacaagcga
     3781 cttttcaact gtcggtatca aggctcttga tgatcaaacc gttcaataca ccttggctcg
     3841 accagaacct tactggaact caaaaacaac ctcaaccatt cttttcccag tcaatgcaga
     3901 tttcttgaaa tcaaaagggg atgattttgg caaggtagac cctgctaata ttttgtacag
     3961 tggacctttc ttgatgaaag cttttgtttc aaaatctgtc atcgaataca agaaaaatcc
     4021 taactactgg gatgctaaaa atgtctttgt agacgatgtg aaattgacct actatgatgg
     4081 tagtgaccaa gatgctcttg ttcgtaactt tacagacgga gcctatagct atgcccgtct
     4141 ctatccgaat agttcaagtt ttgaagggat taaagaaaaa tacaaagatg atatcatcta
     4201 cagtatgcag gatgctacgt cttattactg gaattttaac ctagatagac aagcttataa
     4261 atttacttct aagacgaccg acattgaaaa gaaatcaact caggaagcag ttcttaataa
     4321 aaacttccgt caagctatta actttgccta tgatcgtacc tcctatgggg ctcaaactca
     4381 gggagaagac ggagcgacta agattttgcg taacttggtt gttcctccaa cctttgtaag
     4441 tatcaaagga aaagattttg gtgaagtagt agcctctaag atggtcaata attatggtaa
     4501 ggaatggcaa ggcatcaact ttgcggatgg tcaagaccct tactacaatc ctgagaaagc
     4561 caaggctaaa tttgctgagg ccaagaaaga gctagaagct aagggagttc aattccctat
     4621 tcatttggat gcgacagttg accaagcttc taagaagggg attcaagaag taagctccat
     4681 gaagcaatct attgaagcag ctttaggtac ggagaacgtc gttattgata tccaacagtt
     4741 atcaacagag gactatgata actcaagtta ccttgctcag actgctattc agaaagacta
     4801 cgacctttac aatggtggtt ggagtcctga ctacttagat ccatcaacct atcttgatat
     4861 ctttagtgtc aagaatggtg gggttctaca aaatcttgga ctcgaccctg gtgaagccaa
     4921 tgacaaggct aaggcagttg gacttgacac ttatactcaa atgttggaag aggctaataa
     4981 ggagcaagac ccagccaaac gttatgagaa atatgctgat attcaagcat ggttgataga
     5041 tagttctcta gcgattccaa atgtttcatt aggtggaacg cctagcttga gaaaaacagt
     5101 tcctttctca gcaccttatt cattggctgg taataaaggg gttgaatcat ataaatacct
     5161 caaagtacaa gataagattg tcacaacaga cgaatatgca aaagccagag aaaaatggtt
     5221 gaaagaaaaa gaagaatcca ataaaaaagc ccaagaagaa ttggcaaaac atgtcaaata
     5281 aaatctaatt gtagataagt ttgtgtaaga agtctttgtg cttaaaaacg cataatatca
     5341 ggtatataaa atcctagatg ttatgcgttt tgttatagga agatttatta gattttatcc
     5401 tcaaattgag ttattcccga ctttatccgt tgaaatatta taaggagtat ttatgaaatt
     5461 aaaattatta agagtagata ctaaggtgat tatgtggagt ttcttacttg ttctgtctag
     5521 tctacttgct ttgttgcttc cccttatctt aaagggttta atagatggga gttctattga
     5581 aaatataggt tccaaagtat ttcaatcgtt tttgattttt attggtcaag ccttgttttc
     5641 ttctattggt tactatctgt ttagtcaatc gggtgaaaaa agatagcaaa aatcaggaaa
     5701 aaagtgatag aggggttgat ttatgcagag aaatccttct ttgataagag ccaaagtggg
     5761 gagttgactt ctgccattgt caatgacatg agtgtcattc gtgagttttt aattacgact
     5821 ttcccaaata ttattctgag tttagttatg gtacttggtt ccattgtagt cttatttagt
     5881 cttgattgga atctttctct acttttattc atcactcttc cttgtatgat gtttattatc
     5941 ttgccccttt ccaatatcag tgaaaagtat agtcgtcgtt tacaggagga aatcggattt
     6001 ttaacagggc aattgactga aaagattcaa gaacatgaat taattaagac taaccaagca
     6061 gagaaaagtg tacaggatgt tttggataat tgtattgaaa gggtacaaaa caattcactg
     6121 aaatcagata gggttacttc ttttgagacc ccgtttgctc tcttatttat ctttgcgact
     6181 atagttgtga tgctaaccta tgggggttat cgggtcagcg caggatatat atctgtggga
     6241 accttggttt cgtttttgat ttacctcttt caattactta atcctattag taatatagct
     6301 aattttgtaa ctatttattc tagtagcaag ggatcttcag ttgcactgga gaacttgctt
     6361 gcagttccta aagaaaaatt tgagggagga aaatcggtat caggacaagg gttgaatttt
     6421 aaccatgtct attttggtta tgatgaaaat cgacctgtct taaaggatat tacttgttca
     6481 attttcaagg ggcaaaaaat tgcttttgtt ggaccatctg gatcaggaaa atcaacgatt
     6541 gtgcgtttgt tagagcggtt ttataaaccg ctttcaggag atattctaat ggagcaatca
     6601 agtatatatg attttaactt aaaagaatgg agaagtaaaa tcgcttgggt ttcacaaaat
     6661 aatgcagtct tatctggcag tattcgtgac aatctttgtc tcggtttgaa tcgcttagta
     6721 actgatgatg aattgatgaa agtgctagac ttagtatcac taggtgatga gattcgctcc
     6781 atgaaagagg gactagatac tgaagttggt gaacgcggac gattcttgtc aggggggcaa
     6841 agccaaagac ttcaaatagc tagaacctac ttaaaagatg ctgaaattct tatatttgat
     6901 gaagccactg ctaatcttga tgcggattct gagtatgcga ttatcagtag cctctattct
     6961 gtattaaagg agaagacggt tgtgattata gcgcatagtt tgtcaacggt aaaagatgtg
     7021 gattgtattt tcttcttaga ggaggggaaa atcactggct caggaactca taaggaacta
     7081 ctggaaaatc atgagcgtta tgctcgtttt gtgcaggagc aaatgataga gtgaaggtgt
     7141 cttttgagat tcaccatttt atagtctatt aaagggagca ggaaaaactc cctttttata
     7201 tagtttgaaa ctataactag ctcttgaaaa gaagaaaatg agttgatgaa aataagtggt
     7261 acaatagtta ctatagattt ggaggtattg tatgagcaag gaattacaca ttaacacaat
     7321 tttggcccag gcgggtatta agtcagatga ggcaacaggt gctttggtga caccacttca
     7381 tttttcaacg acctatcagc atccagagtt tggtcgatct actgggtttg actatacgcg
     7441 cactaaaaat ccaactcgta gtaaggctga ggaagtcttg gcggctattg agtcagcaga
     7501 ctatgcccta gcgactagct cagggatgtc agctattgta ctggccttta gcgtctttcc
     7561 agtaggaagt aaggtcttgg cagtgcgtga tctttacggt ggttcttttc gctggtttaa
     7621 ccaagtggag caggaaggtc atttccattt tacctatgcc aatacagaag aagagttgat
     7681 tgccgagtta gaaaaggatg tggatgttct ctatatcgaa accccaacca atcccttgat
     7741 gttggaattt gatatcgaaa aactagcaaa attggctcat gctaagggtg ccaaagtggt
     7801 ggtggacaat accttctata gccctatcta ccaacgtccg attgaagatg gagcagatat
     7861 cgttctccat tcagcaacca agtatctagc aggccacaat gatgtcttgg ctggagtggt
     7921 tgtgaccaat agtttagaac tatacgagaa gcttttttac aatctcaata caacaggggc
     7981 agtcttgtct ccatttgaca gctaccagtt gcttcgtggt ctcaagacct tatctcttcg
     8041 tatggagcgc tcaacagcta acgcccaaga agtggttgcc tttttgaagg attctccagc
     8101 agttaaggaa gttctctaca ctggtcgtgg aggcatgatt tcctttaaag tagccgatga
     8161 aacacgcatt cctcatattt tgaacagtct caaggtcttc tcttttgcgg aaagtttggg
     8221 cggagtggaa agtcttatta cttatccaac gactcaaact catgctgata ttccagcaga
     8281 agtacgccat tcttatggtt tgacagatga cctcttgcgt ttgtctattg ggattgagga
     8341 tgctagagat ttgattgcag atttgcgcca agccttagaa ggataagaca aagatgggaa
     8401 aatatgattt tacaagcctg cccaaccgtt tagggcacca tacctataaa tggaaagaaa
     8461 cagaaacgga tagtgaagtt ctaccagctt ggatagcgga tatggacttt gtggtcttgc
     8521 ctgaaatccg ccaagccgtg caaacttacg cagaccaact ggtttatggt tatacctatg
     8581 ccagtgaaga cttaattaag gaagttcaaa agtgggaagc tacacaatac ggttacaact
     8641 ttgacaaaga ggctcttgtc tttatcgagg gtgtggtacc agccatctca acagctattc
     8701 aaacctttac aaaagaaggc gaggcggttt taattaacac gcctgtctac ccaccctttg
     8761 ctcgcagtgt caagttgaat aatcgtagat tgattactaa ttccttagtg gaaaaggata
     8821 gtctgtttga gattgacttt gaccaacttg aaaaggattt ggtggaagag gaggttaaac
     8881 tctatgttct ttgcaaccct cacaatcctg gtggacgtgt ttgggaaaaa gaagtgttgg
     8941 agaagattgg ccaactctgc caaaaacacg gtgttttgtt agtttcggat gagattcacc
     9001 aagatttgac cctctttggt cacaaacacc agtctttcaa taccatcaat cctgccttca
     9061 aaaattttgc tatcgtcttg agcagtgcca ctaaaacatt taatattgct ggaacaaaaa
     9121 attcctatgc agtcattgaa aatcctaagt tgagactggc tttccagaaa cgcctgttgg
     9181 ccaataatca gcatgaaatt tcaggcttgg gttatttggc gacagaagct gcctatcgat
     9241 acggtaaaga ttggctagag gaactcaagc aagtctttga agaccacatc aattatgtga
     9301 tggatctatt tggaaaagag actaaaatca aggtcatgaa accgcaaggt acctacttga
     9361 tttggcttga cttttcagct tatgacctga ctgatgaaac attgcaagag ttgttgagaa
     9421 atgaagccaa ggttatcctc aaccgtggtt tggattttgg agaggaagga agtctccatg
     9481 cccgcatcaa tatagctatg cccaaatctc tgttgcaaga agtctgtcag cggattgtgg
     9541 ctacttttgc caaacgttaa aaatccagcc ttctaggaga aaagtcttcc tagaaggcta
     9601 ttttcatagg cgaaaatatg gtataataaa aagataaggt aaaggtgaaa atatggctaa
     9661 attgattccg gggaaagttc gtatcgaagg tgttgccctt tatgaaactg gtaaggttga
     9721 tatcatcaag gaaaagaaca atcggctcta cgctcgcgtt gcagaagaag aactgcgcta
     9781 tagtttagag gatgatttgg ttttttgtgc ctgtgatttt tttcaaaaga ggggttactg
     9841 tgtgcatttg gcagcgctag agcattttct gaaaaatgat gagcgtggtc aggaaatctt
     9901 gtggagtctg gaagaaggtc atgaagaaaa agaggccgtt gaaaccaagg tgaccttggg
     9961 tggcaagttt ttggatcgaa ttttatctcc aaaatcagaa tgcgcctatg agttatcagc
    10021 tgtgggtcag gtggaagcag gaaccaatca tatcttgtgg actctgagaa tcggccaaat
    10081 caatagccaa aaatactatg tcattcggga tattccactt tttttaagga ttgtcgagca
    10141 gagaaagtcg tatatgattg gcaagattta tgaggagtct ctttcttggg aagcctttga
    10201 tgatgccagc caagaacttt tgactttctt gcacggacta acggaggaag ggctggtttc
    10261 agacctcttt ttccaaaacc aaggtcggca cctctttttc cctctgacct tttttgagca
    10321 gggtgtgaat ttactgatgg ccttgcccca ttttcaattt gaccatcaag tggatagcta
    10381 ccaaaccctg ctttttcagg atatgcatgc tgatgccaat ctatttgcct ttacagtaac
    10441 agaatactcg gactattttg aaatggaaat cagtgagagt cctagggtca atgtctttta
    10501 tcagggagca gtacttttcc ataaaggtca ggtttatttt ttaacagacc agcagatgcg
    10561 tcttctcaag gaaattaaag cgttacctgt gggtcaacaa ggaaagaaat acctgcaatt
    10621 tgatagcagt gatcgagaca aattggcctc ctgtctgact ctttttatcc agatgggcac
    10681 cgtttcagct ccagaacgtc tacaaatcaa aacttttgcg ccctcttttt actttgacag
    10741 ggaagaagat aatcggattc gtttggaaat tcagtttgat tatggaaata gacaggtatc
    10801 tagccgacaa gagctagaag aattaccatt ttcaagtgac gcagacttag aagaaagaat
    10861 tttccaagtc tgtttggctg cgggctttga agccgatttt caatcttggc gtcaggcctt
    10921 aaaagcggag tctgtctatc atttttttca tgatatcatt ccagtctttg aaaaactcgg
    10981 gcatgttgac ctatcagata aactagaaga gctttatagc ctagcgagtc ctcaggtgca
    11041 gattgcctcc aagggaggtc tcttggaaat ccagtttgat tttcaagata ttgcccagga
    11101 ggaaatcgac caagccatgc aggccttggt tgccaatcag gatttttata ttgattcgtc
    11161 taatcaagtt tactttttcg atgaagaaac caagaaaatt cgccaaaatc tacaggaact
    11221 ggggcaattt gaattaaaag atgggacctt gcaggctcgg aaatccttgg cctacagttt
    11281 agctcatctc tttgaagggc gagaccgtgt ttctttttca caagaattcc agaatctggc
    11341 ccaggatttg acacatccag aggactttcc tcgacaggca actcaggttc aggctgactt
    11401 gcgagattat caggaaaagg gaattggctg gttgcagatg ctccatcatt atggttttgg
    11461 tgggattttg gctgatgata tgggacttgg gaaaaccctt cagaccattg cttttttgac
    11521 tagtcaagtg acaaaagaaa gtcgggtttt gattctcgct ccgtcaggtt tgatttacaa
    11581 ctgggcagat gagtttcaga aatttgcccc acagttggat gtggctgttg ttcatggttt
    11641 gaaagcaagt cgtgaggaga ttcttgccga gagccatcaa atctatgtga ctagctatgc
    11701 caccttccgt caggacagtg agttttatca ggggatggcc tttgacttcc ttttcttaga
    11761 tgaggctcag gtcatgaaaa atgcccagac caagattgcc cagaccttga gacaatttgt
    11821 ggtgccgtcg gtctttgcct tgtcaggaac tccgattgaa aaccatctgg gtgagttgtg
    11881 gtctattttc caaatcgtca tgcccggact tttgccaagc aagaaagaat ttatgaaatt
    11941 gccagcagag cgagtggctc agtttatcaa gcctttcgtg atgcgacgca agaaagaaga
    12001 agttctgact gaattgccag acttgattga ggtggtttat aaaaatgaac tggaagacca
    12061 gcaaaaggct atttaccttg cccagttaca acagatgcga gaccatctgg ctcaagtgtc
    12121 agaacaggaa tttcagcgaa gtcgtgtgga aattttatct ggtttgatgc gcttgcgtca
    12181 aatatgtgac actcctgccc tgtttatgga agattatcag ggagccagcg gcaaactaga
    12241 tagtctccga gacctactgg tacaggtggc agacggcgga caccgtgtct tgattttctc
    12301 tcagttcaag ggaatgttgg aaaaaattga acaagaactg ccagacttgg gcctgacatc
    12361 ctttaaaatt acgggttcaa ccccagccaa ggaaagacaa gacatgacca aggcctttaa
    12421 ccaaggagaa agagatgcct ttctgatttc cctcaaggct ggtggggtcg gtctgaacct
    12481 gacaggtgca gatacagtga tcttggttga cctttggtgg aatcctgcgg tggaagcgca
    12541 agccattggc cgtgcccatc ggatgggtca ggaagaaacg gttgaggtct atcgcttggt
    12601 gaccaaggga accattgaag aaaaaattca ggaactccaa gaacaaaaga aacatctggt
    12661 gtcacaagta ttggatggca cagagtcacg tggtagtctg accctagcag aaattagaga
    12721 aattttggga atttctgaag ccagcacttg aaaaatcaag acaatacgct ataatgaagt
    12781 gttgaaagga aatagaaaat gaaacaagaa cgatttccat tggtgtcaga tgacgaggtc
    12841 atgttgactg aaatgccagt catgaatctc tatgatgagt ctgatctgat cagtaatatc
    12901 aagggtgagt atcgagataa aaattattta gaatgggctc ctattactga agaaaaacca
    12961 gtaaaaccga ttgaaaagca agtcgaaaaa cctaaaaagg ctcctttagg ggttaaaaaa
    13021 gaaggaaaga gctatgcgga ggtggcgcgt gaagaagcgc gtgcggactt gaaaaagaaa
    13081 cgctctgcta actacctaac tcaggatttc agccttgcga gacgtcattc tcagcccagt
    13141 ctagttagac agggcaatca accgacaact cctttccaaa aggaaaatcc tggtgaattt
    13201 gtcaaatata gccaaaaatt gacccagtct cattatatct tggcggaaga agttcattct
    13261 atccctacca agaatgaaga agtgtcagca cctgctccaa agaaaaacaa ttatgatttt
    13321 ctaaagaaga gccaaatcta caataaaaaa agtaaacaaa cagaacaaga acgtcgggtt
    13381 gcccaagagt tgaatctgac cagaatgaca gaatagggga gaaaacatgt caaagacata
    13441 tcattttatc ggaattaagg gatcagggat gagtgccttg gccttgatgt tgcaccagat
    13501 ggggcacaag gttcagggat cagatgttga aaagtactac tttacccaac gcggtcttga
    13561 gcaggcagga attaccattc ttccttttga tgaaaagaat ctagacggtg atatggaaat
    13621 tatcgctgga aatgcctttc gtccagataa caacgtcgaa attgcctatg cggaccaaaa
    13681 tggtatcagc tacaaacgtt accatgagtt tctaggtagc tttatgcgtg actttgttag
    13741 catgggagta gcaggagcac atggaaaaac ttcaacgaca ggtatgttgt ctcatgtctt
    13801 gtctcacatt acagatacca gcttcttgat tggagatggg acaggtcgtg gttcggctaa
    13861 tgccaaatat tttgtctttg aatctgacga atatgagcgt cacttcatgc cttaccaccc
    13921 agaatactct attatcacca acattgactt tgaccatcca gattatttca caagtctcga
    13981 ggatgttttc aatgccttta acgactatgc caaacaaatt accaagggtc tttttgtcta
    14041 tggtgaagat gctgaattgc gtaagattac gtctgatgca ccaatttatt attatggttt
    14101 tgaagctgaa ggcaatgact ttgtagctag tgatcttctt cgttcaacaa ctggttcaac
    14161 cttcaccgtt catttccgtg gacaaaactt ggggcaattc cacattccaa cctttggtcg
    14221 tcacaatatc atgaatgcga cagccgttat tggtcttctt tacacagcag gatttgattt
    14281 gaacttggtg cgtgagcact tgaaaacatt tgccggtgtt aaacgtcgtt tcactgagaa
    14341 aattgtcaat gatacagtga ttattgatga ctttgcccat catccaacag aaattattgc
    14401 gaccttggat gcggctcgtc agaaataccc aagcaaggaa attgtagcag tctttcaacc
    14461 gcataccttt acaagaacca ttgccctgtt ggacgacttt gcccatgctt taaaccaagc
    14521 agatgctgtt tatctagcgc aaatttatgg ctcggctcgt gaagtagatc atggtgacgt
    14581 taaggtagaa gacctagcca ataaaatcaa caaaaaacac caagtgatta ctgttgaaaa
    14641 tgtttctcca ctcctagacc atgacaatgc tgtttatgtc tttatgggag caggagacat
    14701 ccaaacctat gaatactcat ttgagcgtct cttgtctaac ttgacaagca atgttcaata
    14761 ggatgttccc atggaaattc caattaagat cattcaggca agcaagtttg atttgcctga
    14821 gataggggca cttcagacct cgtcttttcc agctgaaaag cagcaacttt cccatatttt
    14881 agaagaaagt atccgtaagt gtgcggatac ctttcttcta gctagggatg aaaatcaact
    14941 tttaggctat attttatcaa gtccccagtc agacaatccg caatgtctaa aagtacattc
    15001 tttagtcatc gagtctgacc atcagagaca gggcctggga acacttcttc ttgcagcctt
    15061 gaaagaggtg gcagttgagc tggattacaa agggattcgt ttggagagtc ctgatgagct
    15121 gctttcctat tttgaaatga acggttttgt tgatgaagaa gaaactttgc tctatgcaac
    15181 tagccagggc tatagtatga tttggtttaa tcccttttat ctggaggaac aatgaaaatc
    15241 agacaagcaa gattagaaga tttggatcgg attgttgaac ttgaatttga aaatttttcg
    15301 gtcgaagaag ccattcctcc ttctgtcttt gaagcacatt tgagagaaat tcagacctct
    15361 tttctggttg ctgaaaaaga aggaagaatc atgggttata tcgaaggacc agttggcctg
    15421 caccgccatc tgcaagacca gtcttttaca gaagaaataa aagactatag tcatgagcct
    15481 ggtggttata tctctgtgac ctgtctgtct attgccaagg aggcacaggg actcggactg
    15541 ggtcagaaat tgctgacagc cttgaaagaa gttgctcttg aagatgagag agatggcatt
    15601 aatctaacct gtcatgacta tctcatcgcc tattatgaaa aacatggatt tgtcaacgaa
    15661 ggccagtccc agtcaacctt tgcaggggaa acatggtatg atatggtctg ggaaatgaaa
    15721 aaataagtta ggaaaagtat catagattgc tttttttgga cttttaagat ataatattat
    15781 ggattatcaa caaggaggaa aaacttttga gtgaaaagtc aagagaagaa gagaaattaa
    15841 gctttaaaga gcagattctg agagatttag aaaaagtaaa aggctatgat gaagttctga
    15901 aagaagatga ggcagtagtt cgcactcctg caaatgaacc ttcagctgaa gaactcatgg
    15961 ctgattcctt gtcaacggta gaggagatta tgagaaaagc tcctaccgtg cctactcacc
    16021 caagtcaagg tgtaccagct tctccagcag atgagagtca aagagaaact cctggtgttc
    16081 caagtcatcc aagtcaagat gtaccttctt ctccagcgga agaaagtgga tcaagaccag
    16141 gtccaggtcc tgttagacct aagaaacttg aaagagaata caatgaaacc ccaacaaggg
    16201 tagctgtttc ctatacgacg gcagagaaaa aagcagaaca agcaggtcca gaaacaccta
    16261 cgcctgctac agaaatagtg gatatcatca gtgatacatc acgtcgtagc cgtagagaag
    16321 gagcaaaacc cgttaagcct aagaaagaga agaagtcaca tgtgaaagct tttgtgattt
    16381 cattccttgt attccttgcc ttgctctcag caggtggtta cttcggttac cagtacgtgc
    16441 tagattcttt gttacctatc gatgctaatt ctaagaaata tgtgacggtt ggaattccag
    16501 aaggttcaaa cgttcaagaa atcggtacga cgcttgaaaa agctggtttg gtaaagcatg
    16561 gtctgatttt tagtttttat gccaagtata aaaattatac cgacttgaaa gcaggttact
    16621 acaatttgca aaagagtatg agtacagaag acttactcaa agagttgcaa aaaggtggaa
    16681 cagatgaacc gcaagaacct gtacttgcga ctttgacaat tccagaaggt tataccttgg
    16741 ataagattgc tcaagctgtg ggtcaattgc aaggtgactt caaagagtct ttgacagcgg
    16801 aggctttctt ggctaaagtt caagatgaga cgtttatcag tcaagcagta gcgaaatatc
    16861 ctactttact ggaaagtttg cctgtaaaag acagcggtgc gcgttatcgt ttggaaggat
    16921 accttttccc agctacatac tctatcaagg aaagcacaac tattgagagc ttgattgatg
    16981 agatgttagc tgctatggat aagaacctat ctccttacta tagtactatc aaatctaaaa
    17041 atttgactgt caatgagttg ttgaccattg cttccttggt cgaaaaagaa ggtgccaaga
    17101 cagaagatcg taagctcatt gcaggtgtat tctacaatcg tttaaatcgt gatatgccac
    17161 ttcaaagtaa tattgcaatc ttgtatgccc aaggaaaact ggggcaaaat atcagtctag
    17221 ctgaggatgt tgcgattgat accaacattg attcacctta taatgtttat aaaaatgtag
    17281 gtctcatgcc tggtccagtc gatagtccaa gtctggatgc gattgagtca agcatcaatc
    17341 aaactaagag cgataacctc tactttgtag cagatgtcac agaaggcaag gtctactatg
    17401 ctaacaatca agaagaccac gaccgcaatg tcgctgaaca tgtcaacagc aaattaaact
    17461 aaacaaacta aaattatgtg atacgtcaca taattttctt tagaaaatat tatcagaaga
    17521 aagttgagaa aaatggcaga aaaaacatat cctatgaccc ttgaggaaaa ggaaaaactt
    17581 gaaaaagaat tagaagaatt gaaattggtt cgtcgaccag aagtggtaga acgcattaag
    17641 attgcccgtt catacggtga cctttcagaa aacagtgagt acgaagcagc taaggatgaa
    17701 caagcctttg tcgaaggaca aatctctagc ttagaaacaa aaatccgcta tgctgaaatc
    17761 gtcaatagcg acgcagttgc ccaggacgaa gtagcgattg gtaaaacagt caccatccaa
    17821 gaaattggtg aggacgaaga agaagtttat attatcgtag gttcagctgg tgcggatgcc
    17881 tttgcaggta aggtttcaaa tgaaagccca attgggcagg ccttgattgg caagaaaaca
    17941 ggtgacacag caaccattga aacgcctgtt ggtagctatg atgtaaaaat cttgaaggtt
    18001 gaaaaaacag cctaaaaaca gaaaaaggag tggggaggcg atgtgcttca ctcactcctt
    18061 tttccatttt gctattcttc gaaaatctct tcaaaccacg tcagcgtcgc cttgccgtat
    18121 gtatggttac tgactttgtc agtttcatct acaacctcaa aacagtgttt tgagcaacct
    18181 gcggctagtt tcctagtttg ctctttgatt ttcattgagt attagatgta tagtgcattg
    18241 aagttaaaat agtaaacttt gatttctaag acattattag aaattagatt aaattcctta
    18301 atcgatttgc gcatgtttta tttcatttca ttatacttta gattggaaaa taaggaatca
    18361 cataaatcgc aagaaatagg gaatctgatt cgtgtttata accgttcaaa aagggaagag
    18421 gctgaaagtg agccacttaa tctttatgtc gaagatgaaa agggcaatct ccttgcgggt
    18481 ttgatagcag agacttttgg aaattggcta gaaatcgagt atttgtttgt aaaagaggaa
    18541 ctgcgagggc aaggaatcgg ttcaaaacta ttgcaacagg cagaaagtga agctaagaat
    18601 cgaaattgtt gttttgcctt tgtcaatact tatcagttcc aggcaccgga cttttatcag
    18661 aaacatggct acaaggaagt tttttctttg caagactatc tctacattag gcaaagatat
    18721 tattaccaaa agaatctgta aggtgagtat ggaggtataa tacaaagaga ggctcgcaca
    18781 taagtatgga ggtgtactga tgtatatgag taactatcga taaataccag ttataaccta
    18841 gaaattgtgt ctttctagac gatattgagg acaatataat cgtagctcca aaattaggaa
    18901 tcaaggctta tcaggttaag aaaagaagtg atgttgttga tattttaaaa caatttattt
    18961 aaactaaaaa ctctctatct ttttaaattg acattaatcc gagattcccc tctaggattt
    19021 tgtgttgctt tcattaaatt tacaggtaag acttgctagc ttcagtaaat atacacaagt
    19081 ttttcagaat agagtgaaat ccgaaagaaa atggaaataa tgaaaactct tgtaggcata
    19141 agaaaacacc tctgtgttat acttgttgtt caaccacaaa cacaagaaag gcacagaaat
    19201 gcaagacaat tata
//
LOCUS       DAVAAL010000026        18998 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVAAL010000026 DAVAAL010000000
VERSION     DAVAAL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18998
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.027"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(104..466)
                     /locus_tag="VQM60_000760"
     CDS             complement(104..466)
                     /locus_tag="VQM60_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080648.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(757..2637)
                     /locus_tag="VQM60_000761"
     CDS             complement(757..2637)
                     /locus_tag="VQM60_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET1080649.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLATVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2631..3500)
                     /locus_tag="VQM60_000762"
     CDS             complement(2631..3500)
                     /locus_tag="VQM60_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET1080650.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3584..6229)
                     /locus_tag="VQM60_000763"
     CDS             complement(3584..6229)
                     /locus_tag="VQM60_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET1080651.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGHPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNRPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDDTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEYALECHQAQERFSAYRSAKALQTPFTSLQLARQEGS
                     VAATGSLLSHSVLSIPQICPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLDPQEIRTEFIKKEEI"
     gene            complement(6320..7600)
                     /locus_tag="VQM60_000764"
     CDS             complement(6320..7600)
                     /locus_tag="VQM60_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET1080652.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDSLLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPFQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADTKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7771..9855
                     /locus_tag="VQM60_000765"
     CDS             7771..9855
                     /locus_tag="VQM60_000765"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET1080653.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKSSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(9895..11574)
                     /locus_tag="VQM60_000766"
     CDS             complement(9895..11574)
                     /locus_tag="VQM60_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET1080654.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPAYG
                     NGGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDDLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12330..13559
                     /gene="arcA"
                     /locus_tag="VQM60_000767"
     CDS             12330..13559
                     /gene="arcA"
                     /locus_tag="VQM60_000767"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET1080655.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13617..14633
                     /gene="argF"
                     /locus_tag="VQM60_000768"
     CDS             13617..14633
                     /gene="argF"
                     /locus_tag="VQM60_000768"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET1080656.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14798..15745
                     /gene="arcC"
                     /locus_tag="VQM60_000769"
     CDS             14798..15745
                     /gene="arcC"
                     /locus_tag="VQM60_000769"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET1080657.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15956..17467
                     /locus_tag="VQM60_000770"
     CDS             15956..17467
                     /locus_tag="VQM60_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET1080658.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17489..18820
                     /locus_tag="VQM60_000771"
     CDS             17489..18820
                     /locus_tag="VQM60_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET1080659.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQLKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHKRSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            18915..>18998
                     /locus_tag="VQM60_000772"
     CDS             18915..>18998
                     /locus_tag="VQM60_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET1080660.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRF"
BASE COUNT         5336 a         4305 c         3924 g         5433 t
ORIGIN      
        1 attacgaatg aatttgattg ctggtattat caatcatgaa ctaggattct agttttgcag
       61 gaagtctatt gaaatactaa gcctacacag actgatttca attttaccat tcttcatagt
      121 caaaaccatc ataatctgga tattgttctg gcgacgttcc aaccgaacgt acgagataag
      181 cttcttcgtc ctctgtctcg atttctctca gcacttggca ttcaaaacgc cattcatcac
      241 cgaagtcgaa aataaactta aatttttgtt tgacacttag gctatccaga tagacatttt
      301 ctgtgtaacg ttcttcaaca tcgtcactaa caaagctaag aaagtaagaa tctgcatgac
      361 tccactcaac attatccatg aaaaatgcgt gagcatgatc atttgcaaaa tcaaaactcc
      421 ataaaatcac atctgctagt tccgctaacg tttttgtttt tggaatctat atatgtcggt
      481 agcaaccttt tttaaatttg acggaaatga cccaagcttc tacagatgct ggcttttcag
      541 cttctatttc ttgttttact aattttaact gtagcatttg aaaccagata attcttctgt
      601 ttcacgcgaa acaccctgcg ccaagcaagg tgttcttgtt tttatactca atgaaaatca
      661 aagagcaaac taggaagcta gctgcaggct gctcaaaaca ctgttttgag gttgtagatg
      721 gaagctgacg tggtttgaag agattttcga agagtattaa gtcgtataaa tcgtcgaagc
      781 tgtcgcaatg gtatgccact ggttagctgt agttgctgcg aaatccttgt ctgcgtagaa
      841 gtcagtaaat ggtaggattt caacttccag ctcgtcgatg cggtcaagct ggccagccag
      901 ataaacctcg atacggcttt ctgctcgttt gatgcgttgc aagagtccgc ccatacggat
      961 gtcaactgta tccaaaccaa agaccttgtt ttctttcagc cattggtgac taaagagggc
     1021 atggaagtct tcaatttggc ttctaagttc tggtaattct tgtctggcga tttgttgtaa
     1081 actttcttta tcatccgctt ggtaggcctg acgaatgcgt cgtcccacat ctactttgct
     1141 acttaaaata gcattcaact gggcctgagt ttcaaagaga taagcatagt ttccagcttt
     1201 ttctttaatg tcagcaatag ttcccgccgc ctgagcgaag tgtggcttgt cctgttcagg
     1261 tgtcatgtgt cggtcaagta tcggacagag aacatcctga taaaagacat atcggttggg
     1321 attgatgcca ctgagattac ctggtaggtc tggtaagagg ttggcaaggt caatctgcat
     1381 aaaatcttca accgttagac cagtattggt cttgaagtgc gcagacaaac tatctaggtc
     1441 attgcgatag ctgagttctg cccagatttg caagcttggt aggatagaga actgggcagt
     1501 ttcaccacca ttgtctcccc aacccgttac gatgacttct ttaatctgat tggcacggca
     1561 ggctttatta gcctcgatag ccactagacg gctaaaatgg ttgtgaggtg taaagccaat
     1621 ccacttccaa gctccccctg caaatgcaag gtcatggcta atcttgtgat gattgcggaa
     1681 attacggttg tatttttcct cgctatcctg ataataatcc cagtaaacca gagtcacacg
     1741 gtctttgaga cggtctaggt agacacgagt ttcctctgga atttccacat cacgatcgta
     1801 ctggccatcc gctgacatga gtttgaagaa catatcactc cacatctggc agtggaaacc
     1861 atatttgtca gcaatatcca gcacgcgctc caagtgttgg cacatgagga gactacgatc
     1921 cacaacaccg ttcagaatca ggtagcgtcc caaaccaacc aagtgggctt cgtccatccc
     1981 gatattgacc ttgcgagtct tcagtttaga caacgtggca aacatgccat caatcaggtc
     2041 ataaaccttt tcttcgccaa tgagaagaat gtcctctaca tcacggagct cctgcacttc
     2101 cttgacaccc catttgacaa aggccgacaa gtgggccaag gtctggatgc atggtacaaa
     2161 ggtcatgtca aactgttgag cataggcttc gatttcctgc aactcctctg ctgaataagc
     2221 tccacggaaa tagccaaagt aaggctgtcc ttcaatctgg taagtgtctt ccatgtaaag
     2281 ctcaaaggtt gagtagccca tgagagccaa gacctcaatc atctgcttgg cagaagcgac
     2341 attcagcacc gcatttcgag aacagtcaac catgtaagcc aaatcttcgt aagctgcttg
     2401 ttcctcaatc tctactttat cagcttctgc tagaactgtt gccaacaagg acaaggcacg
     2461 ataaagttgg tgaggtttgc ggtaggtcaa ttgatagtga ccttcctcac cctcgataga
     2521 gatagaggct tggtcagact gagtgacagc cacttccaca tctggtagag aaatatgacc
     2581 ttttaaaacc tcaatagctt gcgtttgttt gggactaagt cctgtaaatc ttaccattgc
     2641 ttttcctcct gtagccagtt gacaagagca ccgtagagat tggcatctgc gtgataggtg
     2701 caggcctgga taactggtgc gaccgtgtat tcttcgtagg catcgacaaa gtcttcaaca
     2761 gccttcttga caccttggat aaaatctgga ttttgactga tagagccacc cagactgatg
     2821 acatctggat cgatgagata ctggatattg agcaagcctt gcgccagatt gcggttcatt
     2881 cgctcaatgg cttcttgaca aagagcatta ccagctgcgg cctcttggta aatcttgcga
     2941 ccgtcccaat cagtatgacc agatttttca atcacgtatc gtaccatatt cccagttgat
     3001 gctagttgcg accagttatt aagtttttca gcaggggcaa gggttgtcat gtagccaaat
     3061 tctccaccca gaccgtggcg acctcgatga agtctaccat tgataatcat ggctccgcca
     3121 atccctgtcc caatcacgac acaggctgca ttttcaagct ctggatgagc tagtagttca
     3181 ctgagtccaa cgcagttggc atcattttct aaatggacag gtagctgata agagctaagc
     3241 gcctcatacc aagaaaagcc atggatgtag ggcaccgcac tgaagccatc aatcacacct
     3301 gtctcttgat tgaccgcacc cggaacgctc atagcaatcc cactgtaatc ctgttctgac
     3361 aagcgttgat ctagccacgc tagtaaatcc tccaagtttt caggcgttga aatacttgtc
     3421 ttatccagta ttttcccatc aggagtcaga ctggcaaact taatcccagt ccctccgata
     3481 tcaatcgttg caatggtcat tgtttttacc ataaaaaggg gcgaatcagc agttagttct
     3541 tactttttcg cccctccttt cttttgatgt ttactttttg aaattaaatt tcttcttttt
     3601 tgatgaattc tgtacgaatc tcttgtggat ccaatagtcc tgaatgaact ggatatggtc
     3661 gctcaagtaa gtcaagaatg gtttgttggt gttctgatat gcgcacattt tcttgactca
     3721 tattgtagta acggagtaca tatccttctt cattttccgc cactttaaag gctgttggac
     3781 agatttgcgg tatgctgaga acagaatgac tcaagaggct accagtcgca gccacgcttc
     3841 cttcctgtct agcaagctga aggctggtaa acggtgtctg caaggctttg gcactacgat
     3901 aggctgagaa gcgttcttgg gcttggtggc actcaagcgc atactcgact tcaaactcac
     3961 gcaagcattg ggcttctggt gttgggaagt agccccagtc acctagctca cctgatgcac
     4021 gcaaaatagt cactgcaatg gtgtcgtctc caaggatttc gtattcattc aatcccttgt
     4081 tggatacagt cacacctttt tcatcgtcat acagactaac aaaggcttgt tggtgttgag
     4141 gattttcagg attttcccat gaagcagctg gtctgtttgg acgtgtcacc acctcataaa
     4201 tgctttcaga atcattgctt ggacgcgtgt tatgagtctt gaccaagaga cggatacggt
     4261 gatccttggc agtgttagta aagcgagtct tgaagcggat ttgtggattg tcaacgaaga
     4321 cagtcaactc agtttccaga ggaatgtttg tcaattcttc tgaccgtcca gcctcacgct
     4381 tcataaactc gatgatacct tgttgctctt cttctagctt ttcatccgcg ctgacaggca
     4441 cggtcaattc atgtttgagc aagattttag catagcaagc tgtgttttcc aagacctcgt
     4501 ggcccttaag ctctgcaaag attggctctg ttccttttgg ttggaaatag atatactcgt
     4561 ttccgatgtc cccacggtct tcaaagcgga taaagtcttc ataggcttcg tgagttgtct
     4621 tgtcatagac tgtgatgttg tcatccacac tcaccgttac gaatggtgta tcaatcactc
     4681 cgttttggta aataccctca cggtgttctt gttttccttc cagcaattgg aaggttgtcc
     4741 aagaaagcgg cgctaggtga actggaatgg tcacgcgcac ttgacgagca atacgagctt
     4801 ggcggaactt gtcttttggt aaatcatact caaaattagc tccgaggtct tcgattgtag
     4861 cctctacagg atgaccatcc aagtcctcca cacggtaact tggcaagata agagcagcca
     4921 tctttttgta gccttctgtt gggtgcaatt ccttgaaatc acaagtcgcc acatcaatca
     4981 ctgtgctgac agtatcgacc ttatcatgca agcctgtgtt aatgacagta aagagatagt
     5041 cactttgagc cttatccgta gcaattttac ccttccactc gttgagcaag ttacttttaa
     5101 caaagtttcc tacttggttg accttggcaa aacgcgtttc catctcgcgg tgaacttcgt
     5161 ccacgctaca gccacagata ctatcatgtg gcgcgttctg caaaagtgtt ttccaagcat
     5221 aggtcaactg gtccttgtgg ttgtgtccac cagtgataat agtcaagggt tctaccactt
     5281 gctctaggag gttgctattt tcttggaagg cttgttttag gtaaatgcgg gatgaagaag
     5341 tgttggcaag tgtgtaccag ccatctgttt cctgactggt caactcacct gtaacagttg
     5401 ataagtgttc aggaagcgca ccttctacag cttgaacata ttcatcaaaa gaactatgaa
     5461 caaagattac atctgggaag agttcatttg ccacacgaat ggcttcgctc agatttttct
     5521 gtacaggctg gtggtcacag ccgttcatca tcaaccattg gttggtcgaa gcgtaggcac
     5581 gcacatctga caatttttgt ttccagaagg tcaaggcctc atctttgtca actggaattt
     5641 cattcccgtt actgtaccag ttggcaaaga gaatacctaa aacacgacta ccatccacac
     5701 cctgccagta catttctgaa aactgggatg taaactgctc atcttcaagg acttggttgt
     5761 caaatccaat cggcttcaca ccacgaccaa aggccgccac gtgaatgcct gatttttgaa
     5821 gaatttgagg cgcttgtccc atatttccaa aggtatctgg aaagtagcca atctgggttg
     5881 atttacccca tttggcagct tcttgttgac caatcaaggt attgcggaca ttggcttcac
     5941 tggagatcaa gtagtcatcc tgcaagatgt aaaagggacc aattttaagt ttgccctcgt
     6001 caatgtagcg ttggaccttg tcacgatttt cagggcgaat ttgtaagtag tcgtcaagga
     6061 caatagtttg tccatccaag tggaaactct tgaactcagg gtcattttca aagagatcaa
     6121 agagattgtc aaacaattcc accaactgca tacgatggct ttcaaaaggc aagtaccact
     6181 cacggtccca gtgactatgt gagataatat gtacaacaac attttccatg aagtaaaacc
     6241 tcattctaaa tttaaatttt aacgttttaa atgtaaactt gtgattctga taagaatcgg
     6301 ttgagctaaa gcgagcccct tagcgaatat ccaagtaatc caagaccaac tcacagaaca
     6361 tcatgttagc ccaggagaac cattcacgag agtagagggt cggatcatct acgtggaagc
     6421 tttcgtgcat gacacctgta ccaccatcgc aggcaaccag ctgatccagc aagaattttt
     6481 tctctgcctt atctcttgtt gtcaatcctt ggatagaaag ggcgattggc cagatatagc
     6541 gatagaaggt atgagaactt ccgagaccgc tagcgtattc tccttggtag aagtatggat
     6601 tttcagagct caaaatggta cgacgagtag cttgatacac ttcatcatcg accgaacagt
     6661 agcccagata gggcgcagct agtagacttg gtacatttgg atcatccatg atgctggcat
     6721 ttcctaggcc atccacttca aaagcgtaaa tcttttcgcc cttgctgttg gtggtgtaag
     6781 cgtagttttc gattccttct tggatttcat cctgaagacg cttggtatca gcaataacac
     6841 tctggctatc agctaggttt aatgctgcga agatttcttg cacataaccc aagactacta
     6901 cagcaaacat atttgacgga atcaagtaac tatactggca acagtcatca ctcggacgaa
     6961 aggctgacca agtcatacct gtcactgcaa agtcaggtcc aaagccatca tttaccaagg
     7021 tgtcttcctt acggtccgta tctcggacaa aacgataagg agagttcttg tggtcttgtt
     7081 caactgtcca tagatggaga atttccttag tcgctgcgac aaaagtctca tcaaactgac
     7141 cagtctcgcc agtctctttc cagagaagat aagccaactg aaaaggatag caaagcgagt
     7201 ccacctcata cttgcgttcc caaatccagc cattaaggtc ggtgtggtcg gtctcgtggt
     7261 gccccttcca gttttcctct atgttgaagg aattggcata aggatccttg agtaccaagg
     7321 tcatctgacg tttgaccaaa cctgcaatgg tctgacgcag gagggaatct cttttagcca
     7381 catgcaggta gggtctgagt tgggctgtcg aatcccgaag ccacatagca ggaatatccc
     7441 cagtcaagac aaaagttgaa ccatcttcta agatttcaac cgtattgtcc aaggtatcgg
     7501 tgtagcaacg ctcaaagaca tcaacccact ctggatggtc cttggcccgc tctgctactt
     7561 catccagcca ctctctgacg atttctttcg aataaatcat cgtgcaattc cctctttcaa
     7621 acaagtttca tgttcagtat atagcttttg agacagaaaa tacatacaca aatgatagtc
     7681 atttttctac aaaatagtta tttttgaaac tccttttcta aaggccctct ttttctgata
     7741 taatgtagaa aaacagctaa aggaaagact atgaaaccac tacttgaaac catcgatacc
     7801 cgctttggaa ctaccaacaa gcatgccttt tctcggggaa ataccctgcc atacacaggc
     7861 gtgccttttg ggatgaatta ctttgtgccc cagaccagtg accaggatgg atcttggttt
     7921 tttgatccgc atctgcctat ctttcagggg attcgattaa ctcaccagcc cagtccttgg
     7981 attggggact actcttggct ccttctgaca cctgtcacca gtcaactggg gggagacagc
     8041 ctctttcacc gccagtcttc ttatgatata gataaagctt gcttccagcc tcactatttg
     8101 aagctctttt ctctgcgtta tcagattgaa acccagctca caccgacttg ctatggtgct
     8161 tctattcgtt tgaatcaaaa gcaaggcaaa gccctctccc tctatcttca cgcagcagat
     8221 gaactgacag tagagcaagt agataagcgg actcttgccc tgcgacaaga aggtaaaact
     8281 gaaactaaca aaaattcgct aacgatgttc actgccctgc aaatgaacac ggatattctt
     8341 gccatcagcc aagaagctgg agactggcga attgacttag caagtagtca aaccgagatg
     8401 caactagcca cttctttcat ctctccttct caagcactgc ttaatctacc tcaagaagat
     8461 tttgatagct gtaaatcaag tgcccaagcg gattgggaaa atctcctcca tcgttttgac
     8521 attatagaga taggagaggc tgaccgaacc ttctttgacc actgcctcta cagactcttc
     8581 ctcttcccac agacttttta tgagattaat gaatcagggc aagccatcca catggatctg
     8641 gctactggta ctgtcaagcc tggtgtcctc tttagcaaca atggtttctg ggataccttc
     8701 cgcaccacct tccccctctt tgcccttatc ataccggagc actatcaacg ctttttagaa
     8761 ggtttcctca atagctaccg cgatactggt ttccttccaa aatggctggc tccagatgaa
     8821 cggggtatga tgccaggtac acttttagac ggtattatcg cagatagcgc ctgcaaggac
     8881 atggcccctg acctagaagg agaactcttc caagccatgc tcgaaacagc cagcaaagcc
     8941 gaccctctcg gaatcaatgg ccgccacgga ctagcccaat accaagaact aggttacctc
     9001 tctaccgacc accacgaaag tgttagccat actctagact atgcctatag tgacttttgt
     9061 atcgccagct gtgccaaaaa actagagaac atagaaatcg ctgaaaccta caaggctgct
     9121 tcacaaaatt accgccagct atttgacgct gagacaggtt acatgcgagc acgagacaat
     9181 caaggaaact ttcaccctga cttctctcct tatagttggg ggcgagacta cgctgaatgc
     9241 tctgccattc aagctacttt aggcgttctc cacgacatcc ctggcttaat ccaactgatg
     9301 ggtggaaaag aaacctttag caactatctt ttgaaagcct gtcaagatgc tcccctcttt
     9361 gaaacaacag gctatggtta cgaaattcac gaaatgagcg agatggctac tgctcctttt
     9421 ggacaaatag ccatttccaa ccagcctagt ttccacattc cttatctctt ccgctacagc
     9481 gattaccctg actacactgc ccttcttatc aagacgctcc gtcagaaagc ttttcaccca
     9541 agttgggaag cctatcctgg agatgaagac aatggtagtc tctctgcttg gtacatctgg
     9601 tcagctctcg gattttatcc aacctgtcca ggaaaaccaa gctatgacct cggaatccct
     9661 ctctttgacc atctacgagt ctacctcgct aaagaagata aatggctgga tatccatact
     9721 aaacaaaacc acaaccattt taactttgtc aaagaatgcc gactggacaa aacactcgta
     9781 tcaactattc aacaccaaga cctcttaaaa gctgaacaac taactttcac cctcagctgg
     9841 ttaccaagtc actaataacc aaaagaacct ttgcatcgcg caaaggttct tcttttatga
     9901 aacttggact tgaagttgat ccacaagctg accatccacc gttaggttca gatagaaatc
     9961 caaggtttta tctcctgcga aatacagggt tggtagatgc aaatcttttt tgctttcccc
    10021 tgcttggaat tgaaggactt gaatctcatc ctgataggcg acaccatgga cacctgtccc
    10081 cggttgaatc gaaatcttag cctctaaaga ggagctactt tcactacgtt caatcctaaa
    10141 atgaagagtc tctccctttg ccacagctag gcttttttct gcaaatgcta gcccctgaac
    10201 agctactgtt tgtgataacc tcggagtttt atagagtgaa atcttggtca acaaaggcaa
    10261 agcctgtgat tctgtaatga ctacacgtat cttctgtgct tcaacaactg ctcctcgtaa
    10321 gagacgtttg taaccaacag tatgacccga accaaactcc tgccagacac catccacctc
    10381 tacttgcaca tgaaaagcag cgattcgttg ccctagcttc aaatcttctc ttaactcaat
    10441 tacatcaaaa gttttaggag aacctaagtc gagttctaac tggatgggca agtctgcatc
    10501 gtttgcccaa gagctggtct caaggtcgtc tgtcaaatgg cgacaagcaa agtctgcgga
    10561 aagagctgga ccagatacct cagctcccag agccaaatct tctttataga gctcattgcg
    10621 ataggtcgca aattcataaa gtcgttcaat atcctttgca tcaaagagcc cagcttgatt
    10681 cggcggaata ttaagcaaga gtggagttcc tcgccctact gagtgaaagt agatttcgac
    10741 caactcctcg agagacttag gatcctgatc ctcatggtag aaccagcctg gacggatgga
    10801 aacatctgcc tctccgattg aaaaaatcgt gcccgaggga tccccgtgct gaagatagtt
    10861 cagctctgct tctgttccta gtttatcagg attcaccttt tgccacagtg gatcacctgc
    10921 ataccctcgt tcattgccaa tccagcggat actggtgcct tctgttgaaa aaatcaagca
    10981 agcgccctgc aggtcacgaa tggtttcaaa ccatttttca aattcataat taaccttttg
    11041 cgcgccctct cctctggcac catccatcca aacctcagca aatttccctc catttccata
    11101 ggcaggattt gataagattt ccttcagctg agccagataa taggcattgt agtccgcttc
    11161 tcggtccaca tgatagaggg gactatgggc atcccacggt gacaggtaga cccccatatc
    11221 catatcaaac tctgtggcag cttgggatac ttcaaggagc aagtcgccct ttcctctcct
    11281 ccaaggactg accttaaccg aataatctgt gtgagctgtc ggataaagga caaagccatc
    11341 gtggtgcttg accaccaaaa tcaacttttt gaagcccgtt tccttgagca cacgaaccca
    11401 ctcacgcgca tccaactgac tcgggttaaa gcgctcagga tcctcctgtc cagtccccca
    11461 ttcttggtca taaaaggtat taggaccgaa gtggataaaa gctgctagtt catctcccag
    11521 ataagctagc tgagtctgac ttggtaacgg tccatgcggt ttgattttct tcatcgttat
    11581 atatctcctt ctttagcaac ttctcttgtt cacaaggtta tccacaggtt gtggataata
    11641 aaccatgatc taacagccag ctctttccaa ctccaactgg taggctcagg attttgctcc
    11701 tcaatattcc ctcttcaaac agctctgtca tatggttctg ctaacacctc tcagaatgct
    11761 actctccata attattctca ggcttctttc aggtcattgt gtgaagacca ttcactctcc
    11821 agacttttcc acagattgtg aataagtgat tattctaaac tatcacctcg tcgactataa
    11881 caaaaaataa aaagagggtc aataagataa ttatctcttc ctaaatactc agaaaagatt
    11941 ctataatttg tacaattgaa caagaaatgc ttctccaaaa tctatcttca aaagaatgtt
    12001 gcatcttttt atcctttcac acaaacattt ctctttatac agatccaagt gataaccaga
    12061 tgataattgg acactttttg gttatttgtg tattttttga aaaatttttt aaaaaaattt
    12121 tatatcctct ttaatagaag tttatgcatt tgtatatatg tattccatcc tatgttgtgt
    12181 ataataacat aaaattgtgc taaatatcat aatttatatt attttccgag aaattatagc
    12241 cattgacatt acctaggaat tgcgttataa catacatgta agcggtaccc aaaatggtgc
    12301 caagtcaaaa tttttaagga ggaaaataca tgtcttcaca tccaattcag gtcttctcag
    12361 aaattgggaa actgaaaaaa gttatgttgc accgtccagg caaggagtta gaaaacttgt
    12421 tgccggacta tcttgaaagg cttctttttg atgatattcc tttcttggaa gatgctcaaa
    12481 aagaacatga tgcatttgcc caagctcttc gcgatgaagg aattgaggtt ctctacctag
    12541 aacaactcgc tgctgaatca ttgacctctc cagaaatccg cgatcaattt atcgaggaat
    12601 acttagacga agccaacatc cgtgatcgtc aaaccaaggt tgctattcgt gaattgcttc
    12661 acggcatcaa ggacaaccaa gaattggttg aaaaaacaat ggctgggatt caaaaagttg
    12721 aattgccaga aattcctgac gaagctaaag atctaactga cttagttgaa tcagattatc
    12781 catttgcaat tgacccgatg ccaaacctct atttcactcg cgacccattt gcaacaattg
    12841 gaaacgccgt atcgcttaac cacatgtttg cagacactcg taaccgtgaa acactctacg
    12901 gtaagtatat cttcaaatac cacccaatct atggcggaaa agtggatttg gtctacaacc
    12961 gtgaagaaga tacgcgtatc gaaggtggag acgagctagt tctttctaaa gacgtccttg
    13021 cagtaggtat ctctcaacgt acagacgcag cttctatcga aaaacttttg gtcaacatct
    13081 tcaagaaaaa tgttggcttc aagaaagttt tggcctttga atttgctaac aaccgtaaat
    13141 tcatgcactt ggatactgtc ttcactatgg tagactatga caagttcact attcacccag
    13201 aaatcgaagg cgaccttcac gtttactcag ttacttacga aaacgaaaaa cttaaaatcg
    13261 ttgaagagaa aggtgactta gctgaacttc ttgctcaaaa ccttggtgta gaaaaagttc
    13321 atttgattcg ttgcggtggt ggcaatatcg tagcagctgc gcgtgaacaa tggaacgacg
    13381 gttctaacac tttgaccatc gcacctggtg tggtagttgt ttatgaccgc aataccgtga
    13441 ccaataagat tttggaagaa tacgggcttc gcttgattaa gattcgcgga agtgaattgg
    13501 ttcggggccg tggtggacct cgttgtatgt ctatgccatt tgaacgtgaa gaagtgtaat
    13561 cgctgttcgg tattcgtcaa tagaaaatgt aaaaaataga aagaggaaat aataaaatga
    13621 caaattcagt attccaagga cgcagcttct tagcagaaaa agactttacc cgtgcagagt
    13681 tagaatacct tattggtctt tcagctcact tgaaagattt gaaaaaacgc aatattcaac
    13741 accactacct tgctggcaag aatatcgctc tcctatttga aaaaacatct actcgtactc
    13801 gtgcagcctt tacaactgcg gctatcgacc ttggtgctca cccagaatac ctcggagcaa
    13861 atgatattca gttgggtaaa aaagaatcta ctgaagatac tgctaaagta ttgggacgta
    13921 tgtttgacgg gattgaattc cgcggattca gccaacgtat ggttgaagaa ttggcagaat
    13981 tctcaggcgt tccagtatgg aacggtctaa ctgacgaatg gcacccaact caaatgctcg
    14041 ctgactactt gactgttcaa gaaaacttcg gtcgcttgga aggcttgaca ttggtatact
    14101 gtggtgatgg acgtaacaac gttgccaaca gcttgctcgt aacaggtgct atccttggtg
    14161 tcaatgttca catcttctca ccaaaagaac tcttcccaga aaaagaaatc gttgaattgg
    14221 cagaaggatt tgctaaagaa agtggcgcac atgttctcat cactgaagat gctgatgaag
    14281 cagttaaaga tgcagatgtt ctttacacag acgtttgggt atcaatgggt gaagaagaca
    14341 aattcgcaga acgtgtagct cttcttaaac cttaccaagt caatatggac ttagttaaaa
    14401 aagcaggcaa tgaaaacttg atcttcctac actgcttgcc agcattccac gatactcaca
    14461 ctgtttatgg taaagacgtt gctgaaaaat ttggtgtaga agaaatggaa gtaacagacg
    14521 aagtcttccg cagcaagtac gctcgccact tcgatcaagc agaaaaccgt atgcacacta
    14581 tcaaagctgt tatggctgcg acacttggta acctttatat tcctaaagta taattttaga
    14641 taataaaccg tctaccaaca gctatgaggg ctgcgactaa tagctttagt ccggtcctct
    14701 tttatgtaat ggtaatctat tatttcttat aaaatatgtg aaaaatcatt aaattgaaat
    14761 ctaaacgcat tctattgagt gtgataaagg agaatttatg gcaaatcgta aaattgtagt
    14821 agctttggga ggaaatgcga ttctttcttc tgacccatca gcaaaggctc aacaagaagc
    14881 tttagttgaa acagctaagc atcttgtaaa attgattaaa aatggagatg atctgattat
    14941 cactcacggt aatggacctc aagttgggaa tctcttgctc caacatttgg catcagactc
    15001 tgaaaagaac cctgccttcc cactcgactc acttgtcgct atgacagaag gtagcatcgg
    15061 tttctggttg aaaaatgctt tgcaaaatgc tctcttggat gaaggcatcg aaaaaaatgt
    15121 tgcctctgtt gtaacgcaag ttgtcgtaga taaaaatgat ccagcttttg ttaacttgag
    15181 taaaccaatc ggtcctttct attcagaaga agaagcaaaa gcagaagccg aaaaaagcgg
    15241 agcgactttc aaggaagatg ctggccgtgg ctggcgtaag gtcgttgcct caccaaaacc
    15301 tgttgacatc aaagaaattg aaaccatccg tactctttta aataatggtc aagtcgtcgt
    15361 agctgcaggt ggtggcggta ttcccgtcgt caaagaaaac aatggacatt tgactggtgt
    15421 cgaagcggtt attgataaag acttcgcttc ccaacgtttg gcagaattgg ttgatgcaga
    15481 cctcttcatc gttttgacag gtgtagatta tgtatttgtt aactacaaca agccaaacca
    15541 ggaaaaattg gaacatgtga atgttgccca gctggaagaa tatatcaaac aagatcagtt
    15601 tgcaccaggt agcatgcttc caaaagtaga agcagctatc gcttttgtca atggtcgtcc
    15661 agaaggaaaa gcagttatta cttcccttga aaatctaggc gccttgattg aatctgaaag
    15721 cggaacaatt attgaaaaag gataagttgt tttactaata agatgtattc tatttctagt
    15781 atctttatat caaattagaa attattcttg aaaacatgta caatatttca aaagatatta
    15841 gttttttaga ctttaatatg gtaaaacaaa tataaataga aagcgttttc ttaaatgttt
    15901 atttaagaaa gtagttggtt ttttacactt tgttagacat caggaggaaa aacaaatgag
    15961 tgaaaaagct aaaaaagggt ttaagatgcc ttcatcttac accgtattat tgataatcat
    16021 tgctattatg gcagtgctaa cttggtttat ccctgcgggg gcctttatag aaggtattta
    16081 cgagactcag cctcaaaatc cacaagggat ttgggatgtc ctcatggcac cgattcgggc
    16141 tatgctaggt actcatccag aggaaggttc gctcattaaa gaaacgagcg cagcgattga
    16201 tgtagccttc ttcatcctta tggttggggg gttccttggc attgtcaaca aaactggtgc
    16261 tcttgacgta gggattgcct ctatcgtgaa gaagtataag ggccgcgaaa aaatgttaat
    16321 tttggtactg atgcctttgt ttgccctcgg tggtacaact tatggtatgg gtgaagaaac
    16381 aatggccttc tatccactcc ttgtgccagt tatgatggcc gttggttttg atagcctgac
    16441 tggtgttgca attattttgc tcggttctca aatcggctgt ttggcatcta ctctgaatcc
    16501 atttgcgaca ggtattgctt cagcgactgc gggagttggt acaggggacg gtatcgtact
    16561 tcgtctgatc ttctgggtta ccttgactgc tcttagtact tggtttgttt accgttatgc
    16621 ggataagatt caaaaagatc cgactaagtc actggtttat agtactcgca aagaagattt
    16681 gaaacacttt aacgtagaag aatcttcatc tgtagaatct acacttagca gcaaacaaaa
    16741 atcagttctc ttcttatttg tgttgacatt catcttgatg gtattgagct tcattccatg
    16801 gacagacctt ggcgttacca tttttgatga ctttaatgct tggttgactg gtcttccagt
    16861 tattggtaat attgtcggtt catctacttc tgcactaggt acttggtact tcccagaagg
    16921 cgcaatgctc tttgccttta tgggtatcct gattggtgtt atttatggtc ttaaagaaga
    16981 taagattatc tcttccttca tgaatggtgc tgctgacttg ctcagtgttg ccttgatcgt
    17041 agcgattgct cgtggtattc aagttatcat gaacgacggt atgattaccg atacaatcct
    17101 caactggggt aaagaaggct tgagcggtct atcttcacaa gtctttatcg ttgtaactta
    17161 tatcttctat ctacctatgt cattcttgat cccatcttca tctggtcttg ccagcgcaac
    17221 tatgggtatc atggctccac ttggagaatt tgtaaatgtc cgtcctagct tgattatcac
    17281 tgcttaccaa tctgcttcag gtgtcttgaa cttgattgca ccaacatctg gtattgtgat
    17341 gggagctctt gcacttggac gtatcaacat tggtacttgg tggaaattca tgggcaaact
    17401 cgtagtcgct attattgtag tgaccatcgc ccttcttctc cttggaacct tccttccatt
    17461 cctataaaat agtgagtgag gtgattccat gaaaatagat ataacaaatc aagttaaaga
    17521 tgaatttctt atatcattaa aaaccttgat ttcctatcct tcagtactca atgaaggaga
    17581 aaatggaaca ccttttggac aagcaatcca agatgtccta gaaaaaactt tagagatttg
    17641 tcgagacata ggtttcacta cctatcttga ccctaaaggt tattacggat atgcagaaat
    17701 cggtcaggga gcagagcttc tggccattct ctgtcatttg gatgttgttc catcaggtga
    17761 tgaagcagat tggcagacac cgccatttga agcaactatc aaagacggct gggtattcgg
    17821 acgtggtgtc caagatgata aaggcccttc gctcgcagct ctctatgcag taaaaagctt
    17881 gctggaccaa ggtattcagt tgaaaaagcg cgtacgcttt atctttggta ccgatgagga
    17941 aaccctctgg cgctgcatgg cacgctacaa taccatcgaa gaacaggcca gtatgggctt
    18001 tgcacctgac tcatcttttc ctctgaccta tgctgaaaaa gggcttctac aggtcaaact
    18061 tcatggccct ggatcggatc aactagagct tgaagtagga ggcgccttta acgttgtacc
    18121 agacaaggcc aactaccaag gtcccctcta tgaacaggtt tgtaacgatc tcaaagaagc
    18181 tggttatgat taccaatcca ctgaacaaac cgtaacggtt ctcggagtgc caaagcatgc
    18241 taaggatgct agtcaaggta tcaatgctgt catccgacta gctaccattc ttgctcctct
    18301 ccaagaacac cctgctctca gctttcttgc aacacaagca ggtcaagacg gcacaggaag
    18361 acaaatcttt ggtgatatag cagatgaacc ttctggtcac ctatccttta atgtcgcagg
    18421 tctcatgatc aatcataaac gttctgaaat ccgtattgac attcggactc ctgtcttagc
    18481 tgacaaggaa gaactagtag agttgcttac aagatgtgca caaaactacc aactccgcta
    18541 cgaagagttt gactatctag cgcctctata cgtcgcaaaa gacagtaaac tcgttagcac
    18601 actgatgcaa atctaccaag aaaagactgg cgataacagt cctgctattt catccggtgg
    18661 tgccactttt gctcgcacca tgccaaattg tgtagccttc ggcgccttat tcccaggagc
    18721 gaagcagaca gaacatcagg caaatgaatg tgccgttcta gaagatttgt accgtgctat
    18781 ggatatttat gccgaagccg tctatcgact tgcaacttaa tcaggcaact gtttctacaa
    18841 aaaaaaatcg accgattaat gaactgcacc ccaaaagtta gacagaataa atctaacttt
    18901 tggggtgttt tattatgaaa ttgagttatg aagataaagt tcagatctat gaactaagaa
    18961 agcaaggaca aagcttcaaa cagctttcaa aaagattt
//
LOCUS       DAVAAL010000027        18545 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVAAL010000027 DAVAAL010000000
VERSION     DAVAAL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.028"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(400..819)
                     /locus_tag="VQM60_000773"
     CDS             complement(400..819)
                     /locus_tag="VQM60_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HET1080661.1"
                     /translation="MTPEQVKETLEGVKWINKEIEGLYLELATLESGIIKKQELSNTR
                     VQTSRVNTAENNLISVLKLKEDTLQRIERLTEERMEISRLIDKLANPLERSVLRLFYL
                     NVLEAWQVAEEIDRSKTTVYLIRQKAIEHLAGLVHAD"
     gene            complement(1438..1689)
                     /locus_tag="VQM60_000774"
     CDS             complement(1438..1689)
                     /locus_tag="VQM60_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080662.1"
                     /translation="MKAIKNRLNKLNKLAVVDEVDTVFLTQIDTGHFEVRYRIGGKNP
                     KDIIEVYPTKEEAEKAISEIKNTKGLRIFWDNLALLEDN"
     gene            complement(1676..2017)
                     /locus_tag="VQM60_000775"
     CDS             complement(1676..2017)
                     /locus_tag="VQM60_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HET1080663.1"
                     /translation="MANYLMTGNVKKASELSEITRKTAYNYLNDATFKRIYRERRSEQ
                     LKESTTLLQNASVEAVNVLREIMLDKTVSPYARQQSAQSILNMAYKAVEMVEVVEQLE
                     ILEAKILDEGD"
     gene            complement(2068..2334)
                     /locus_tag="VQM60_000776"
     CDS             complement(2068..2334)
                     /locus_tag="VQM60_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080664.1"
                     /translation="MVRSLKARLEKLEEYAGKTEYATTDECISLYVDCLRFAFQQDEI
                     KIDMDDFIKTPFKDIKLADYKKSKKRMDELREMTGISEFSELEQ"
     gene            complement(2397..2921)
                     /locus_tag="VQM60_000777"
     CDS             complement(2397..2921)
                     /locus_tag="VQM60_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080665.1"
                     /translation="MTLKTFSDKAKTFNFTYEFKDQDTAQVAGSALMGYMIGTYVVPS
                     ISITYKNKGTLVAEYVEDHKLNKTFKRIRDGFKDYYKQPVNDEAFEERYKRERVLQLK
                     ESEDFESLLNKVTDYELKLLDYAERLLSDKPILMDSMTAFGTLEMLGNESINLFQKLD
                     VEGEYKGLADYSGQ"
     gene            complement(3014..3211)
                     /locus_tag="VQM60_000778"
     CDS             complement(3014..3211)
                     /locus_tag="VQM60_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080666.1"
                     /translation="MAMNICQRVIRLEETVERISEALDGFIENANHNAIVSNKNIERI
                     QESFEIVFKELDELKAVPDKH"
     gene            complement(3531..4985)
                     /locus_tag="VQM60_000779"
     CDS             complement(3531..4985)
                     /locus_tag="VQM60_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HET1080667.1"
                     /translation="MWELYSRIWELGDQWRKENSYIVNEGKKNERVEIPRPSVAIVAK
                     ALQEICHFTFIGEGVISDISKLYLYHLDLGHYVSSNDIFRKLLLKYDSRLTSNKFFLE
                     LISYIRTETKMKPPLDDYRYIPVANGVYNIKTHKLEEFSPNFVITSKIQTEYNPCARK
                     PILDGWFDFDRWLEALAVNDKEVVALLWQVINEAINPNRTRKKMVLMVGDGNNGKGTF
                     QALLENLIGRSNISNLKPDQFGKEFYLGALEGKVCNIGDDISNKYLDEVSDLMSVISG
                     DPVQVNKKSLQPVEARFRLLCIFSGNDLPRARNKTMGWYRRLCIIPFRADFNGQKERH
                     EIKDRFIKNKELLEWVLFKVLNMPDFDSFIEPEAVQKMLSKYKNDNDYIKVWVENFYI
                     PNGWHEINHVPMFIARNKLKEFAEDIGIDKPKLGQFSNAVMAELEKTTSYKYSAKNGT
                     VSSKFYDVLDPQGFQRKRFVKGIWGIHLEDEDLG"
     gene            complement(5065..5925)
                     /locus_tag="VQM60_000780"
     CDS             complement(5065..5925)
                     /locus_tag="VQM60_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HET1080668.1"
                     /translation="MTIYEARGFQSNLVYPFDKIEPFQYIERFKPLVVPESADPEEYK
                     RTQAPYCLSGKVMPEKNGSYKRNNTSLIYRDLIFLDYDDIQGTTEDFIEAVSSALFGY
                     SYILYPTIKHSIEKPRFRLVVKSNNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPATYQKIVEHGLDYPVPKVEPRAKQGTTERYKPRVSGQRSMTMRIIDTLF
                     NGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTFSS
                     IARAEYRKRG"
     gene            complement(5922..6194)
                     /locus_tag="VQM60_000781"
     CDS             complement(5922..6194)
                     /locus_tag="VQM60_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080669.1"
                     /translation="MFSLSRESEQDLAHGILEVVERYLEAREKVPPRLTKLINRVELK
                     EELKISDNTLNKWESQGLRRYQPPDDGSRMIYYLVTDIWKFLGVDE"
     gene            complement(6187..6528)
                     /locus_tag="VQM60_000782"
     CDS             complement(6187..6528)
                     /locus_tag="VQM60_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET1080670.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGSEISLTTKLTERTVRDIIKR
                     LVVKHNIPIVGVRCGVFSGYFIPANKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            complement(6509..6802)
                     /locus_tag="VQM60_000783"
     CDS             complement(6509..6802)
                     /locus_tag="VQM60_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080671.1"
                     /translation="MSYIVKIYLDSEKIPDEPYFTKEIFLATCMGNATKLAVTMGLLT
                     KQALDLETKTAILKALDTIIETQKAILQGVASGQITFVNKSKGEEGLDESIRE"
     gene            complement(6799..7014)
                     /locus_tag="VQM60_000784"
     CDS             complement(6799..7014)
                     /locus_tag="VQM60_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080672.1"
                     /translation="MNELDISNTQAAVLIVILIGLLLYLNHRDRKKSAQIERENQQTI
                     KTPSEDLNPEYGRYIQLAGVNVWGGME"
     gene            complement(7007..7273)
                     /locus_tag="VQM60_000785"
     CDS             complement(7007..7273)
                     /locus_tag="VQM60_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080673.1"
                     /translation="MTKEEIRNILDELDDLTLGLNTAENAFDATFHFFSKNISTKEKT
                     MLLTLEYDTQSAIFNLAYDCLINAKENLVNTIRMYRKELEEKHE"
     gene            complement(7291..7431)
                     /locus_tag="VQM60_000786"
     CDS             complement(7291..7431)
                     /locus_tag="VQM60_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080674.1"
                     /translation="MILDLGKMTQAEFDEVMADIKARNPNLLQLITDFLDRKVTPEEV
                     DD"
     gene            complement(7443..7943)
                     /locus_tag="VQM60_000787"
     CDS             complement(7443..7943)
                     /locus_tag="VQM60_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080675.1"
                     /translation="MISDKLEAISTNLEEIQKSLVGAETALAKREISRVLNDLDDVYS
                     ELTSTEYHEQQQTIKEQTERMKQRVIAEVIGELEEREESYYRNFWGDTQFIKLLSNFD
                     GFLFLNTYLAEITKENTYPMEQSQLLNYVWELIAVDIAKKKRGKKNVLDSWTDSSLYT
                     RGMMID"
     gene            complement(7940..8080)
                     /locus_tag="VQM60_000788"
     CDS             complement(7940..8080)
                     /locus_tag="VQM60_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080676.1"
                     /translation="MEETTYDIIAKSLDRISMELHQADENNDFSRIWVLSGQLKGQWR
                     KT"
     gene            complement(8345..8494)
                     /locus_tag="VQM60_000789"
     CDS             complement(8345..8494)
                     /locus_tag="VQM60_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080677.1"
                     /translation="MTNYDRAKRFYSRATYVLSDFLHGRIDEQEFRKQWQRLADKAVN
                     ETKKN"
     gene            complement(8481..8627)
                     /locus_tag="VQM60_000790"
     CDS             complement(8481..8627)
                     /locus_tag="VQM60_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080678.1"
                     /translation="MEQYTKRASALIITFNRGVISQDEFIEEFTKLREKVVKAVSEAK
                     NDKL"
     gene            complement(8631..9059)
                     /locus_tag="VQM60_000791"
     CDS             complement(8631..9059)
                     /locus_tag="VQM60_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080679.1"
                     /translation="MRKKHQVVKFKDIAEKLPELEGKRLEEITKVLGYRNLESCRTNL
                     CKLKQNNRLDFTLEKGVYSKFALLDGTVKEELEDKELSERGRYLKSVDRYKAMLNAFS
                     IAFDSTVKAETRQKAEHDGLKALDRIPDKHYALLYDMMEG"
     gene            complement(9213..9386)
                     /locus_tag="VQM60_000792"
     CDS             complement(9213..9386)
                     /locus_tag="VQM60_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET1080680.1"
                     /translation="MTDKIKKVGRPKGENSNTVKLTVRVSEQLNSKLEEYGKKNGLTK
                     PETLRKAFENLTK"
     gene            complement(9625..9873)
                     /locus_tag="VQM60_000793"
     CDS             complement(9625..9873)
                     /locus_tag="VQM60_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080681.1"
                     /translation="MENEFKTVTNAKGVEIPKYFKDFKKLVEMDRQLAEYLCMNYELL
                     DSEDLGAFLETVEQGFSWILELIESKDLLYSPQTGKKA"
     gene            complement(9885..10490)
                     /locus_tag="VQM60_000794"
     CDS             complement(9885..10490)
                     /locus_tag="VQM60_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HET1080682.1"
                     /translation="MELVYMDGKKEPYTTSEIIAECAGVTHHTIQELLRKHKADFESY
                     GIIAFEMRKLDGRGRPMKIYRLNEQQATLLITYLRNTEPVRKFKMNLVKAFFEMRDEL
                     SKRYLQRELEKPKRKTLTEAIKSWEKAPQHAYSTLTNLLLKGATGKNKAQLMQERESE
                     NGIDSLTSAELTNYQRLEDMAIAMINLNMRYSEIKELIFKV"
     gene            complement(10512..10889)
                     /locus_tag="VQM60_000795"
     CDS             complement(10512..10889)
                     /locus_tag="VQM60_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080683.1"
                     /translation="MLYHWGLTFSEEISDLEHKGENNMNNKQVYQAQTTDHGIARALG
                     EEIRMITDVEETTYNGHGEIIVEYTSDVALTKLFDRIKALYEKGESYETKTFLHFKVL
                     EDDIEPSPNGVFTAKEVAEFLGI"
     gene            complement(10964..11155)
                     /locus_tag="VQM60_000796"
     CDS             complement(10964..11155)
                     /locus_tag="VQM60_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1080684.1"
                     /translation="MRLTLKALRANSNMKQSEVAQKLGISTTTWSKWENGKSFPDVAQ
                     VKEIEKLFGVAYDDIIFLS"
     gene            11359..12075
                     /locus_tag="VQM60_000797"
     CDS             11359..12075
                     /locus_tag="VQM60_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET1080685.1"
                     /translation="MADNIKRLLSAKGLNPRQLAIALDFKYTTVNDWVNAKTYPRIDK
                     IEMLANFFNVSKSDLVENKNAETPTTSLIQSIYDQLTPPRQAKALTYLKKQLLEQKNE
                     NIVSENIISLDDYRESKTLPVIGVVTAGNGITQDDNLNIEKCFYTDEIPDDYDAIAYV
                     VGNSMEPKIKNGDYLFIKNTPQVDYNTIGIFQVDGANYVKKLRQGYLESLNPDCADIQ
                     LDESNDIRTIGEVVGIYREK"
     gene            12094..12762
                     /locus_tag="VQM60_000798"
     CDS             12094..12762
                     /locus_tag="VQM60_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET1080686.1"
                     /translation="MKFCPECGNSVVGCKFCPNCGYSINASLKTTPQKVATPSSRPST
                     VRSKRTDKVGPLEIDKNHRTYRIHGARKAKGSSGLIGGTAKVVGKTTLALGTGGLSLI
                     PSLIKKDKNDTDWYSFDDLVSYELVINDQSVVSGGVGQALVAGAVFGVIGAVAGGIVA
                     KRKATTKILNMTIRITSNDFTKPVVFIDLIKKPVKNTSKEYKEAIENAQKIIGALDVI
                     THNS"
     gene            12901..13569
                     /locus_tag="VQM60_000799"
     CDS             12901..13569
                     /locus_tag="VQM60_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HET1080687.1"
                     /translation="MTYSQNYLDDILVRMAYHSSGIEGNTISLPETVSIILESTLPRN
                     GKSIREFYEIENHKQAFSYLLDSLANHQALTVGLVQDFHALLVDRLQHDRGQFKQVQN
                     AIIGAEFQTASPAETPYLMTQWADNTAYRLEHAQNEKEVLEILADTHIQIERIHPFSD
                     GNGRTGRLILMYLAMKYLNAPIIIPKDSRAHYMELLANQNVTGLADLFKKSLDYERIR
                     KKQF"
     gene            complement(14033..14491)
                     /locus_tag="VQM60_000800"
     CDS             complement(14033..14491)
                     /locus_tag="VQM60_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080688.1"
                     /translation="MVTDIEQARKNYMADYTPVDLTDAHKLLELAEKLQNEDISINTY
                     ELYKHPEAREKLFWQIAEATYMLLEDTGELQGATPTDEHLKTFVDYLEEKFNNIIKKL
                     IAGTDVAKLQGFLEAVEKDNPHLTKQQKGQLLKDTVVTELFKLWIKRRSK"
     gene            15914..16219
                     /locus_tag="VQM60_000801"
     CDS             15914..16219
                     /locus_tag="VQM60_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HET1080689.1"
                     /translation="MEKWENQDKILLDKNKRGKDRNWRGRKLLSLKLADIFKELGYRE
                     TLIERVETCGDTLRFIRREDGSLRLYQAYFCKNKLCPMCNWRRSMKYSYQTILVRLN"
     gene            16324..16884
                     /locus_tag="VQM60_000802"
     CDS             16324..16884
                     /locus_tag="VQM60_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter"
                     /protein_id="HET1080690.1"
                     /translation="MELQQYDELTFENIKHIDENGVEFWYARDLQKTLEYTEWRNFLL
                     VIDKAKIACDTAKNPIVEHFVDVNKSIPNGYNTTRDIKDFKLTRYACYLIVQNGDPRK
                     EVIALGQTYFALKTRQQELQENFDNLTEEQKRLAIRDEIKHHNKSLSESAESAENATY
                     SLPRATMFNNPEARAFSGFSKSSDLV"
     gene            17320..18486
                     /locus_tag="VQM60_000803"
     CDS             17320..18486
                     /locus_tag="VQM60_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET1080691.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKDGSTVYKAVEIKTYAELVENWLETYCHTVKKSTLMGTKFKIDKYLL
                     PAFGNYRLDKLTPPIIQKQVNQWAKDYNQLGKGFQEYPLLHSLNKRILKYAVSLQAIP
                     FNPARDVIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLKNGTLDVNKTLNIIKEITGPKTKSSIRVIDLDNKTVLMLRLY
                     KARQSQIGKEMGLTYEKVFSNSFDKHIDARMLSFRLVKHLERAGCPRFTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHAKLSMTMDIYSHLSKDNQKNATSFYEKAIEKLKSS"
BASE COUNT         5333 a         3776 c         3210 g         6226 t
ORIGIN      
        1 atctacacgc ttttaaactt gtttatagcg cctaaaccct tgatattact gactttttga
       61 aaaatctcat ttgattagaa atcattaaaa gttcttaaaa attctcaaaa agtccacaaa
      121 aaaagcccca caaaagggca ataaatgacg agttcagcag gcaagaaact agcacggtca
      181 aacgtgcttt ttttattgct ggttgctgat aggtatatta taccatgaag cgcgtggttt
      241 ataagattat gacctcacaa aagtccccct gattcgtttc taagggcttc tagtttatcc
      301 atggtgtttt aatcgtccgg tacaaaacca agcaaaatag ggaggcgaat atgaagccta
      361 gggcacccta tacacattag tctctgtggt aaggttaaat caatctgcat gtaccagacc
      421 agctaagtgt tctatggctt tttgccttat aaggtatact gtagtctttg acctatctat
      481 ttcttcagca acctgccaag cttcgagaac attcaagtag aacagcctga gaacagaacg
      541 ctcaagcgga ttggccagct tatcgatcag cctagatatt tccattctct cctcagtaag
      601 tcgctcaatc ctctgaagcg tatcctcttt tagcttcaga acacttataa gattattctc
      661 tgccgtattt accctgcttg tttgtactct ggtattgctt agttcttgct ttttgataat
      721 accactttct aaagttgcaa gctctaaata taagccttct atctctttgt ttatccactt
      781 aacgccctct agtgtttctt ttacctgctc tggtgtcatg tctcggcctc ctgtatgata
      841 taatagtctt tgtgagaact attagctgag acagagagtg tcttggcttt tttcgttttc
      901 tctatgttcg ctctagcttc aaatgcttcc ccgtgagaaa aaatgtacaa tgacggtgta
      961 aagctcatag aagtgcgtgg ggagggagat agcccccgta tctccttgtg gtttgataag
     1021 caattaaggg atgtttttat ctctcttatt tccttgtcat tctgcaagga attaaggcaa
     1081 gttatttctt tgcttatctg cttatcattt ggtaagcaac aaatccaaaa ataacagact
     1141 tttaaattgc tcttatctcc ttatcatttg gtaggaaaat aaggggagtt attttagttc
     1201 ttatttcctt atcgtttgac aaggagaaaa acgaaaataa ggagctttta aattcgtctt
     1261 aaccccctat cgttttgtaa gcaattaaga aggcttattg tcgtccttat ctgcttgtca
     1321 ttctgcaagg agtagaaccg aaataagagg gctaaaaact tcgcttatct gcttgtcgtt
     1381 tctcaaggag ttaaggaggg ttattatttc tcttatctgc ttgataaatg ataagctcta
     1441 attatcctcc aataaagcca aattgtccca gaatatccgc aatccttttg tatttttaat
     1501 ttctgaaata gctttttctg cttcttcttt tgtcggatag acttctataa tatctttggg
     1561 attttttcca cctatcctat atcttacctc aaaatgtcca gtatcaatct gcgtcaaaaa
     1621 cacggtatca acttcatcaa ccaccgctaa cttattcaac ttatttaaac ggtttttaat
     1681 cgccttcatc tagtatcttc gcctctaata tttctaattg ctccactacc tcaaccattt
     1741 caaccgcctt ataagccatg tttagaatgc tttgggcgct ttgttgtctc gcatacgggc
     1801 tcactgtctt atcaagcatg atttccctta atacattgac tgcctcaacg cttgcattct
     1861 gtaacagcgt agtagactcc ttaagttgtt cgctacgtct ttcacggtaa attctcttaa
     1921 aggttgcatc gttcaagtaa ttgtaagccg tctttctggt gatctcactt aattcactag
     1981 ctttttttac attcccagtc atgagataat tggccataaa tatttctttt tttctgctta
     2041 attctgtcaa atactgttca cctcgcttta ttgctctaat tctgaaaact cacttatccc
     2101 agtcatttcc ctaagttcat ccatacgttt ttttgatttc ttatagtctg ctagcttaat
     2161 atctttaaat ggtgttttaa taaaatcatc catatctatc tttatttcat cttgctgaaa
     2221 agcaaatctt aaacaatcca catataaaga tatacattca tcagttgttg catattccgt
     2281 ctttccagca tactcttcta acttttctag tcttgctttt aaacttctaa ccatccccct
     2341 acctcgtgtg taattctgtc tgatttatgt aaaaaaaggg gattgctccc ctgtgtttat
     2401 tgaccagaat aatcagcaag gcctttatat tcgccctcta cgtccaattt ttggaatagg
     2461 ttaatacttt cattacccag catttccaac gtaccaaaag cagtcataga gtccataagg
     2521 ataggcttat cagaaagcaa gcgttcagca tagtctaata gtttcagctc gtagtctgtg
     2581 accttattca gcaagctctc aaagtcctct gactctttga gttgaagcac gcgctcccgt
     2641 ttataacgtt cttcaaatgc ttcatcgttc actggttgct tgtaatagtc tttgaaaccg
     2701 tcacggatac gtttgaaggt cttgtttagc ttgtgatcct ccacgtactc agctactagc
     2761 gtccctttat tcttgtaagt gattgaaata gacggtacta cataagttcc aatcatgtag
     2821 cccatcaatg cactccctgc cacttgggcg gtgtcttggt ctttaaattc gtaagtgaaa
     2881 ttaaatgttt ttgctttatc tgaaaatgtt tttaatgtca tgttgttttt cctctttctg
     2941 tttttagggt gtcactagta gttacaccat tgcaaggggg tcggtactat ctaccccatt
     3001 ttgtttaaat gttttagtgt ttgtcaggta ctgcttttag ttcatcaagc tctttaaaga
     3061 ctatttcaaa actttcctga atccgttcga tgtttttatt gcttactata gcgttatggt
     3121 tcgcattctc aataaagcca tccagcgcct cactgatccg ctcaacggtt tcttctagtc
     3181 taatcactct ttgacaaatg ttcatcgcca tctggtttgc tccttttttc tcttagatta
     3241 ggttttgatt aggtttaaaa aataaccctt caaaagccta ctatatcaac gtttcaaggg
     3301 tgttagatta gggttagttt tactttttaa gttcgcgcct atataacacg tatatatatt
     3361 attttttata tagagatatt agaaataaaa actaacctac taacctaaat actcgcaaag
     3421 acttttatat caacgtttac aaaggttagt gtttaggtta tgtttctatt ttttgcataa
     3481 cctaaattgc tataaccctt ggtgtaattg gttttttagt attaaatttc ctaacctaaa
     3541 tcttcatctt ctaagtgtat tccccatata cctttaacaa atcttttacg ttgaaaacct
     3601 tgtgggtcaa gtacgtcata aaacttagag ctaaccgttc cgtttttggc tgagtactta
     3661 tatgacgttg ttttttccaa ttcggccatt actgcattgc taaattgtcc aagtttaggt
     3721 ttatctatac ctatatcctc agcaaactct tttaatttat ttcttgcaat aaacattgga
     3781 acatggttta tttcatgcca cccgtttggt atgtagaaat tctctaccca aactttgata
     3841 taatcgttat cgtttttgta cttgctaagc attttttgta ccgcttcagg ttctataaag
     3901 ctatcaaaat ctggcatatt taaaacttta aacaaaaccc actctagcag ctctttgttc
     3961 tttataaatc ggtctttgat ttcatgacgt tctttctgac cgttaaaatc tgcacggaaa
     4021 ggaatgatac aaagccgtct ataccacccc atggtcttat ttctagctct aggcaaatca
     4081 ttgcccgaaa agatacacaa tagtctgaaa cgtgcttcca cgggttgtaa gcttttttta
     4141 ttgacctgca ctggatctcc actaattaca ctcataaggt cagaaacttc atccaggtac
     4201 ttgttagaaa tatcatctcc aatattacaa acctttcctt ccaaagcgcc taaatagaac
     4261 tctttcccaa actggtcagg tttcaaatta ctaatatttg accgtcctat taaattctcc
     4321 agcaaggctt gaaaagtacc ttttccatta ttaccatctc caaccattag caccattttc
     4381 ttacgtgttc ggttgggatt gatagcctca tttatgactt gccacaataa agctacaact
     4441 tctttatcgt taactgctaa agcctctaac catctatcaa aatcaaacca accatctaaa
     4501 ataggctttc tggcgcatgg attgtattcc gtttgtatct tactggtaat gacaaaatta
     4561 ggactaaatt cctctagttt atgagtttta atattataaa ctccattcgc tactggaata
     4621 tatcgataat cgtccagcgg tggtttcatc ttagtttctg tacggatata ggagataagt
     4681 tctaaaaaga acttgttaga agtcaaacgt gagtcatatt ttaaaagcaa ttttcggaaa
     4741 atgtcgttac ttgatacata atggcctaaa tccaaatggt aaagatataa cttactaata
     4801 tcacttataa ccccctcacc tataaaggta aaatggcaaa tttcctgtaa cgctttggct
     4861 acgattgcaa cacttggtcg tgggatttct accctttcgt ttttctttcc ttcattgacg
     4921 atataggagt tttccttacg ccattgatcc ccaagctccc aaatcctact ataaagttcc
     4981 cacatcgttc taggaggttg ttcttgctct ctgacctcca agatttcact ttttaggttt
     5041 gctaattctt ctagttctat ggttctaacc tctctttcta tactcagccc gtgcaatact
     5101 gctaaaagta cggtctagct cctcaatagg caatggttca accgtcacgc tattggctat
     5161 ctttgtcagt tcataagctg tctctaggtc acaatcaacc cacttattaa agagtaagcc
     5221 tacaaatcgt gtcagtgcta cgttgcgccc tccttcgtct ccaaatccgt tgaaaagcgt
     5281 gtcaataatc ctcatagtca ttgacctctg gccacttact ctaggtttat aacgctcagt
     5341 agttccttgc ttcgctcttg gttcaacctt ggggactgga taatcaagtc catgctctac
     5401 aatcttttga taagttgctg ggtcgcctgt tgttactggt agcccttgga gttgtgacca
     5461 ggttaaactg gccatgtcaa agggtagccc aatcttgtca gcaatctctt ttactacctg
     5521 cttataggtt gcctcgttca tcacgttgtt agatttcacc acaaggcgaa aacggggctt
     5581 ttctatgcta tgtttgatag tcgggtataa gatataagag tagccaaaca aagcgctaga
     5641 aacggcttct atgaagtcct cagtcgtccc ctgtatatcg tcataatcaa ggaaaatcaa
     5701 atcccgatag attagactgg tgttgtttcg tttgtaactc ccgtttttct ccggcataac
     5761 cttaccactt aaacaatatg gcgcctgggt tcttttgtat tcctccggat cagcactttc
     5821 agggactacc aaaggcttaa agcgttcaat gtactggaac ggctcaatct tatcaaatgg
     5881 atagacaaga ttactctgga aacctctagc ctcgtaaatt gtcattcatc cacccccaga
     5941 aacttccaga tgtcagttac taaatagtaa atcattcttg aaccatcgtc cggcggttga
     6001 tagcgtctta aaccctgact ttcccactta ttcagcgtgt tgtcactaat tttcaattcc
     6061 tctttgagtt caactctatt tattaacttg gtcaatcttg gcggtacttt ctcacgcgct
     6121 tccaggtatc tttccactac ctccagaatg ccatgcgcta ggtcttgctc gctttctcgg
     6181 cttaggctaa acatatccgc ccacctcctt caaggtttct ttgtagcttt ctaggtcgct
     6241 attcatcaac accgctaggc gcttgctctc ctcctgtact tggttgtaaa aagccttagc
     6301 gccatctagt aactcgcctt tattcgctgg aataaagtac ccactaaaaa cgccacatct
     6361 aacccctaca atagggatgt tgtgcttaac tactagacgc ttgataatgt cacgtacagt
     6421 tctttctgtt agcttggttg tcaggctaat ttctgaccct gttatggagt tctcagcccc
     6481 taccttgatt agtcttaaaa ctcgcttgtc attctctgat agactcatcc aacccctcct
     6541 ctcctttgct tttatttaca aaggttattt gtccgctcgc taccccttgc aaaatagctt
     6601 tttgagtctc tatgattgta tcaagggctt tcaagatagc tgtcttagtt tctaaatcca
     6661 acgcttgttt tgtcaacaag cccatagtta cagctaactt tgttgcattg cccatacacg
     6721 tagctagaaa aatctcttta gtaaaataag gttcatctgg tattttttca ctatcaaggt
     6781 atatcttcac tatgtaactc attccatccc tccccatacg ttgactcctg caagctggat
     6841 atatcgccca tattcagggt ttaaatcctc gctaggtgtt tttatcgtct gttggttttc
     6901 tcgctcaatt tgggcgcttt ttttgcggtc tcggtggttt aaataaagca gtaagccaat
     6961 caaaatcacg attaagacag cagcctgtgt gttacttata tctagttcat tcatgttttt
     7021 cctccaattc tttgcggtac attctgatag tattaactag attttccttt gcattgataa
     7081 gacaatcata agcaaggtta aaaatagcgc tctgtgtgtc gtattccaag gttaaaagca
     7141 tggtcttttc tttggtgctt atgtttttgc tgaaaaagtg aaaagtagcg tcaaaagcgt
     7201 tttcagcggt gttaagtcct aaggttaaat catctagttc gtctaaaata tttctaattt
     7261 cttcttttgt catggttgtt tccttttcaa ctagtcgtcc acctcttcgg gtgttacctt
     7321 tctatctaaa aaatcagtga tgagctgtaa taggtttgga tttcttgcct tgatgtcagc
     7381 cattacttca tcaaattctg cttgtgtcat tttgcctagg tctagtatca ttgcattgcc
     7441 tcctaatcaa tcatcattcc acgagtatat aaggatgaat ctgtccatga atccaaaacg
     7501 ttcttttttc ctcgtttttt ctttgcgata tcaaccgcta tgagttccca tacataattc
     7561 aataattgac tttgttccat tgggtaggta ttttctttag ttatttcagc taggtaagtg
     7621 tttaagaaaa gaaatccatc aaagttggaa agtagcttta taaactgagt atcaccccaa
     7681 aaatttctgt aataagactc ttctcgttct tctagttcac ctattacctc agcaatcacc
     7741 ctctgtttca tcctttctgt ttgttccttt atcgtttgct gttgttcatg gtattctgtg
     7801 ctagttagtt cactgtaaac atcatccaag tcgttcaaca ctctactgat ttctctttta
     7861 gctaatgcag tttcagcccc tacaaggctt ttttgtattt cttctaaatt tgttgaaata
     7921 gcttctaatt tatctgatat catgttttcc tccattggcc ttttaattgt cctgataaaa
     7981 cccatattct agaaaaatcg ttattttcgt cagcttggtg taattccata ctaattctat
     8041 ccaagctttt agcgataata tcgtatgttg tttcttccat acttttacct ctgtttctat
     8101 gtttgtgtaa tcgccttgat ggctttttaa tgcttttttc ctgtcacatg cctcatgctc
     8161 tcggtcgcca aacttctgag cgtggggctt ttttgagttg tttcttatac agtttttttc
     8221 ttgtaagtac tagcctcacg ctcggagtcg ccaaattgaa agcgtgagaa agtaccagtt
     8281 taaagagttg gcgctctatg cttttcaaaa ccttttctaa ttgcttgcct gcacttcggt
     8341 tttcttagtt tttcttcgtt tcgtttacgg ctttatctgc tagccgttgc cattgttttc
     8401 taaattcttg ttcatctatc cgcccatgca gaaagtcgct caggacgtaa gttgcacggc
     8461 tgtaaaatcg cttggctcta tcataatttg tcattctttg cctcacttac tgcttttact
     8521 accttttctc ttagttttgt aaattcttca ataaactcat cttgagatat aaccccacga
     8581 ttaaaagtta taatcaaggc gcttgctctt tttgtgtatt gttccatctg ttacccctcc
     8641 atcatgtcat aaagtagggc gtaatgttta tctgggattc tgtccaaggc tttcaagccg
     8701 tcgtgttctg ctttttgtct ggtttccgcc ttgaccgtac tatcaaaggc gatagaaaaa
     8761 gcattcagca ttgccttgta gcggtctacg ctcttcaaat agcgcccacg ttctgacaat
     8821 tctttatctt ccaattcctc ttttaccgta ccgtccaaga gtgcgaactt gctataaact
     8881 cctttttcta gtgtaaaatc taggcgattg ttttgtttca gcttgcagag gttggttctg
     8941 caactctcta ggttacggta acctaatact ttagtaattt cttctagacg cttaccctct
     9001 aattctggca atttttcagc tatatcttta aattttacta cttgatgttt ctttctcatt
     9061 ctttacctca tttttgggta cgcaaaaagc gcactctatt tctatgtttg acaaataaga
     9121 gtacgcatga taagatattt acgtacctca ttcgtgggga cggatacttt gcaacttctc
     9181 ggattggcgt ttgacaagtt gcaaggtatt ttttatttag taagattttc aaaggctttt
     9241 ctcaaggttt caggttttgt caaaccattc tttttaccgt actcttccag cttactattt
     9301 agctgttcgc taactctgac tgttaatttt acggtattag agttttctcc cttcggacgt
     9361 cctactttct ttattttgtc agtcatcgga ttgcctcctt tcttgacgac ataaattatt
     9421 ataattattg tcgtcttaaa tgtcaacccc taaaccgaaa aaaatttaaa atcttcccag
     9481 cgtaccttat tttattttca atgtactagc ggaaaccgct gtattatgct ataatctagg
     9541 tatagaaaaa tatctatacc cttgatccag tcgcttgctt cagtcgccaa acgttcagca
     9601 agtgactttt ttttattgtc tttttcatgc tttcttccct gtctgggggc tataaagcaa
     9661 atctttactt tcgataagtt ccagaatcca gctgaatccc tgctccaccg tttcaagaaa
     9721 tgcacctagg tcttcactgt ccaataactc gtagttcata caaagatatt cggctagttg
     9781 tctgtccatc tcaactagct ttttaaaatc cttgaaatac ttaggaattt ctactccctt
     9841 ggcatttgta actgtcttaa attcattttc cattttctat actcctatac tttaaaaatt
     9901 agttctttaa tttctgaata tctcatattc aagttgatca tcgctattgc catatcttcc
     9961 aagcgctgat agtttgtcag ttccgcactt gttaaactgt caataccgtt ttcactttct
    10021 cgctcttgca tgagttgggc tttgtttttc ccagtcgctc cctttagcag taggtttgta
    10081 agagtgctat aggcatgctg aggtgctttc tcccatgatt tgatagcttc ggttaaggtc
    10141 ttgcgctttg gcttttccag ttcccgttga agatagcgtt tagaaagttc atcacgcatt
    10201 tcaaaaaagg ctttaaccag gttcattttg aatttacgaa ctggttctgt atttcgtaag
    10261 taagtgatta acaaagtagc ttgttgctcg ttcaagcgat agattttcat tggtcgccct
    10321 cggccgtcta atttacgcat ttcaaatgcg ataattccgt agctttcaaa atcagcttta
    10381 tgcttcctta aaagttcttg tattgtgtga tgagtgactc cagcacattc agcgatgatc
    10441 tcactcgtag tatacggctc tttcttgccg tccatgtaaa ccagttccat gtttttgctc
    10501 cttattgtta atcaaattcc taaaaactcc gccacctctt tagcagtaaa aactccattt
    10561 gggcttggtt caatatcatc ttccaatact ttaaaatgta agaatgtttt tgtctcgtag
    10621 ctttcgcctt tttcgtaaag tgctttaata cggtcaaata gttttgtcaa tgcaacgtca
    10681 ctggtgtact caacaatgat ttcaccgtgt ccattgtaag ttgtttcttc aacgtctgtg
    10741 atcatacgaa tttcttcgcc taatgctcta gcgataccgt ggtcagttgt ttgtgcttga
    10801 tatacttgtt tattgttcat gttgttttct cctttatgtt ctaggtcgga tatttcttcc
    10861 gagaaggtca atccccagtg gtaaagcacc agtggagtat cttgtagatg taatatagta
    10921 ttgcgattgg gttactcctt tctatatccg tttaaatcgg atattagctt aaaaaaataa
    10981 tgtcgtcata agcaacaccg aaaagttttt caatctcttt tacttgggca acatcaggaa
    11041 aactttttcc attttcccac ttactccaag tagttgtaga aattcctaat ttttgtgcta
    11101 cctctgattg tttcatgttg ctattagccc gcaacgcttt caatgtaaga cgcaaatagc
    11161 cctcactcct ttcttttgga tatccgttta aatcggatac aatgacagta tacggtaaaa
    11221 ccgataactt gtcaacactt tttttgattt tattttcttt ttttttgttt cccttgcttt
    11281 ttctacgttt taaccgtata attaaattat gaattataga aaggcggttg cactatgtca
    11341 ttaggaaata agcaaataat ggcggacaat atcaaaagac ttttaagtgc aaaaggttta
    11401 aaccctcgtc aattagctat tgcattagat tttaagtata caaccgtcaa cgattgggta
    11461 aatgctaaaa cttacccacg tatagataaa atcgaaatgc tggctaattt tttcaacgtt
    11521 tcaaagtctg acttagtaga aaataaaaac gcagaaacac ctaccacttc cctcatccaa
    11581 tccatctatg accaactcac tccaccaaga caagccaaag ccttgactta tcttaaaaag
    11641 cagctgctgg agcagaaaaa cgaaaacata gtatcagaaa acattatcag tctggacgac
    11701 tatagggaaa gcaagacgct ccctgttatc ggagttgtaa ccgccggaaa cgggataacc
    11761 caagacgata acctaaacat agaaaaatgc ttttatactg atgaaatacc agacgactat
    11821 gacgctatcg cttatgtcgt cggcaactcc atggagccaa agataaagaa tggcgactac
    11881 ctatttatca agaatacacc tcaagttgac tataacacta tcggtatctt ccaagtagac
    11941 ggcgctaact acgtcaagaa actgcgtcaa gggtatttag aaagccttaa ccctgattgt
    12001 gctgatattc aactagacga aagcaacgat atacgcacca tcggcgaagt cgtgggcatt
    12061 tatagagaaa agtaaaaaag gagtaaaaaa attatgaaat tttgtcctga atgcggaaat
    12121 tctgttgtcg gttgcaagtt ttgtccgaat tgcggatact caatcaatgc ctcgcttaaa
    12181 acaactcccc aaaaagtagc aactccatcg tctagacctt ctactgtaag aagtaaacgt
    12241 actgataaag tcggacctct tgaaatagac aaaaatcatc gtacatatag aatacacgga
    12301 gcaagaaaag caaaaggttc atcaggtctg attggcggaa ctgctaaagt tgttggaaaa
    12361 actaccttag cgcttgggac aggcggtttg tctttgattc catctttaat taaaaaagat
    12421 aaaaatgata ccgactggta ctccttcgat gatttagtat cttacgaatt agttataaac
    12481 gatcagtctg ttgtttccgg cggtgttgga caagctttag tagctggggc ggtatttggt
    12541 gtaataggag ccgtagctgg cggaatcgtc gctaaaagaa aagcaactac caagattttg
    12601 aatatgacaa tccgaataac atctaacgat tttactaaac cagttgtttt tatagattta
    12661 attaaaaaac ctgttaaaaa cacatctaaa gaatacaaag aagctattga aaatgctcaa
    12721 aagattattg gagctttaga tgttattacc cataattcat aaacaaaaat ataccacctc
    12781 atcgaatacc cccttttttc atggtctatt gtgcaaaaac ggggaaaatt gaagaataga
    12841 aagccgattt acagactaaa gcgtaaaatt gggcaaaagt gacaaataga aaggagaatt
    12901 atgacctact cacaaaacta tctggacgat atactagtac gcatggccta ccattctagc
    12961 ggtattgaag ggaatactat ctctctaccc gaaaccgtca gcattatctt agaaagcacc
    13021 ctgccacgca acggcaaaag tattcgtgag ttttatgaga ttgaaaacca taaacaggcc
    13081 ttttcttatc tcttggactc attggcaaac catcaagctc ttactgtggg gcttgttcaa
    13141 gattttcacg ccttattagt ggataggctg caacatgatc gcggacaatt taagcaagtt
    13201 caaaatgcca ttatcggggc ggaatttcaa acggctagcc cagcagaaac gccctacttg
    13261 atgacacaat gggcggataa tactgcttat cgtttggagc atgctcagaa tgaaaaagaa
    13321 gtattagaga ttttagcaga cacccatatc cagattgaac ggattcaccc atttagcgat
    13381 ggtaacggtc gtactggtcg cctgatactt atgtacttgg ctatgaagta tctaaacgct
    13441 cctatcatca tcccaaaaga ctcaagggcg cattatatgg aactcctcgc aaaccaaaat
    13501 gtaacgggac tagctgacct cttcaaaaag tcactggact acgaaagaat acgaaaaaaa
    13561 cagttttaaa acaacaaaaa aaaaagacaa gctagctgct gctgacttgt ctaatatcgc
    13621 ctgaactgaa ataacgggaa agattcaggc gattagtagc atcttgagag attggaagca
    13681 gaaaggtctg gttactgttg agttgggact ctttcaaacc tcttagtcta cgctattatt
    13741 ataccactct tgcatttaaa aagtgactga cggcatttct cacgctcatc agtctataat
    13801 tctctaatga agtataatgc ggtataattt agacgatttt caacacttcg gcattttaga
    13861 aacgcttaaa gcgctctaaa caatatcaaa aaaaataaaa ctcttgaaat attggggctt
    13921 gtatgctaga atgaaagcat aaagggaaaa acagtgcttc tagcatatgt aactaaaaag
    13981 ccccaccgtt acagcgatgg ggttttttgc tgccctctta aagggttaaa tgctacttgc
    14041 tacgtctctt tatccatagc ttgaaaagct cagttactac cgtatctttt agtagttgcc
    14101 ctttctgttg ttttgttaga tgcgggttgt ctttctctac agcctctaaa aagccttgta
    14161 gttttgctac gtccgtgcct gctatcaatt tctttatgat gttgttaaac ttctcttcta
    14221 ggtagtccac aaatgttttt agatgttcat ctgtcggcgt tgcgccttga agttctcctg
    14281 tatcttctaa tagcatgtaa gtagcttcag caatctgcca aaataatttc tctcttgctt
    14341 ctggatgttt gtataactcg taagtgttga tacttatatc ctcgttttgt agcttttcag
    14401 ctagttctag tagtttgtgt gcgtctgtaa ggtctactgg cgtatagtct gccatgtagt
    14461 tctttcttgc ttgttcaatg tctgttacca tgcttacctc tcttgagtgc ctctaaagaa
    14521 aaagtgatgc ccgtttccgc cttttctttt tactggtttt attatagcat acccccactt
    14581 tttaaaagag tgggggggtg ctctttaata gtcttaatag ttttaatagt tttaatagta
    14641 ttgaaaattt tgacccctta gaaaagcagt aatatcaagg gtttacaagt tcgctacctc
    14701 gacgattacc cccctttttt gtaccttttt agtataaatt tgtagtaatt ctttttaaaa
    14761 aatcggacta aaattaaagg aacttaatat ttcttgtaag gttgttggaa taaatagtat
    14821 aaaagatatg aacgagtata aagagattat ggaaaatgtt aatcaagcaa aagaggaatt
    14881 aaagagaata gatgaattaa tagccgtcag gagctccaga attgattttt tagaggataa
    14941 aacccttaga attacaggaa accaaagatg agcttaaaaa cgaaatggag agcctaaaaa
    15001 gagatttatc tttttttaga aacgaaaata ctcgactgaa aaatgtagtt tcagattgta
    15061 tcaaggtagt atttttgggg tatttttagt agaaagtata ttgtaatttc tgagcgattt
    15121 atgccgtgaa agctacttga ccgtaagcta gatcaaagta cgctagtcag ggacgagccg
    15181 aaaggctgta gcgtttcgga gcggcacgga caaagtacgt cgacctaggt tacttgcggt
    15241 caagtagacc atggaataaa acaagcgggt atgatataaa aaagttatcg gcaaaccgaa
    15301 atatagactt gtttttttgt ttataaaaat gtattattat attgagaata agattgttta
    15361 atttatttag agaggagtgt aagttgatca gtaaatgatt ttaattctgc ctttaaataa
    15421 caaggagttt gttatgaata aagcttggtt attttggagt gtcatagtct tatatttttt
    15481 tataaagttt tttgataaag ttttagatat aaagcttttt ggttctattc aaatatggct
    15541 agataatctc cctattccga tgaaaatact gttaacaata gcattagttt tgtttttatt
    15601 cattgtattt ccatatagag gaaaacggta aaaacggtaa ttagatatat aaaaggagct
    15661 gaaagctccg taaaaaacct actcgaagag taggtttttt cttatcttga tactagtagg
    15721 aaataactct catgctattt ttagggtttc gacagggtct aaacccttga tactactgac
    15781 tttttaggta aaaaaatgcc cttgactttt ttcaacggat cttttataat ttagttgttc
    15841 agaaaaaatt ataaaaagag aaaaaaggca aaggtatttt tgtatttctt tgtaattaca
    15901 ggtaaattat aacatggaga agtgggaaaa tcaagataaa attcttttag ataaaaataa
    15961 aagaggcaag gatagaaact ggcgaggacg aaaattatta agtttgaaat tggcagatat
    16021 ttttaaagag ttaggttata gggaaacctt gatagaacgc gttgagactt gtggagatac
    16081 tcttcgattt attcgtcgag aagatgggag tttgcgttta tatcaagcct atttttgtaa
    16141 gaataaattg tgtcctatgt gtaattggag acggtcaatg aaatattctt atcaaacaat
    16201 cttagtcaga ttgaactaga aatcacccat cacaaacaag cccatataag tcccatatct
    16261 actagttttg ttgatgtcaa caaatgtcaa ccacttacaa tattttagaa aggatacttt
    16321 attatggaac tccaacagta cgatgaatta acttttgaaa atatcaaaca catagacgaa
    16381 aatggagtag aattttggta cgctcgggac ttacaaaaga cccttgagta tacagaatgg
    16441 agaaactttc ttctagtaat tgataaggca aagatagctt gcgataccgc taaaaacccc
    16501 attgtagagc attttgttga cgtcaacaag tcaattccaa acggttataa cacaacaaga
    16561 gatatcaaag attttaaact tacccgttac gcttgttacc ttattgtcca aaatggtgac
    16621 ccacgaaaag aagttatagc acttggccaa acctatttcg cgttgaaaac tcgtcaacaa
    16681 gagctacaag aaaactttga caatcttacc gaagaacaga aacgccttgc tatccgtgac
    16741 gaaattaaac atcacaataa atcattatct gagtctgccg aaagtgccga aaatgcgacc
    16801 tatagcctac cacgcgccac aatgttcaac aatcctgaag cgcgtgcttt ctcgggcttc
    16861 agcaagtcct ctgaccttgt ctaaaccctt ctccttttta gggtgttaga aatgttagtt
    16921 ttccaaccat taagaactaa tagaaacggg attggaattg tttaccccat cgctttttag
    16981 ggactcagaa aatgaagaaa attgtttttt tccagtattc ttgaaaatcc gactttttcc
    17041 aacatttatg tgaagactcc gaaaaaagcg tggttgccaa gacctaaaaa acaaacaaaa
    17101 gaaacttaaa aagcaaccgg ataataacca actttcactc tttgatgata tgtaaaaacg
    17161 ctcatacaaa ccccatatcc gccttatttc ctattctggt attatttacc gcctggctaa
    17221 ttaaaatcga aaataggggt attctcgtag cccctcgcat ggtataaact caaaaccttt
    17281 tctaattgct tgcctgctga tggaaaaagg agtaaaacca tgaaaataac agaatacacc
    17341 aaaaaagacg ggtctacagt ctatcgctct agcgtctacc ttggtatcga taccgtaaca
    17401 ggtaagaaag tcaagacgac tatatcaggg cgaaccaaga gagaactcaa agccaaagcg
    17461 ctacaggctc aaatcgactt tgaaaaagac gggtctacag tatataaagc agttgagata
    17521 aagacttacg ccgaactggt ggagaattgg ctagaaacgt actgccatac ggttaaaaaa
    17581 tcgaccttaa tgggtaccaa attcaaaata gataaatatc tacttccagc tttcggaaat
    17641 tataggctgg ataaactgac gcccccaatc attcaaaagc aagtcaatca atgggcaaaa
    17701 gactataatc aactaggcaa agggtttcaa gagtatcctc tccttcactc gctaaacaaa
    17761 cgcatactta aatatgccgt atctttgcaa gctatcccct ttaaccctgc ccgtgatgtt
    17821 atcgtgccac gtcgtaagga aaaagaaggg caaaaactga aatatctgga tgatgataac
    17881 ttaaaaaaat tcttgactta cctggagcag ctgccaaaca cttacaaaaa tttctacgat
    17941 acggtgctat ataagacgct tttagcgact ggtttgcgta ttcgtgagtg tttagccctc
    18001 aaatggtctg atattgacct aaaaaacggc acactagacg taaataaaac gttaaatatt
    18061 atcaaagaaa tcactggccc taagactaaa tcaagtatta gggtaattga cttggataat
    18121 aagacggtgc tcatgctacg gctctataaa gcaagacaat cccaaatagg gaaagaaatg
    18181 gggttgacct atgagaaagt attctctaat agctttgata agcatataga cgctagaatg
    18241 cttagtttca ggttagtaaa acatttggaa cgtgctggat gccctcgttt tacgttccac
    18301 gccttccgcc acactcatgc tagtatcttg ctcaatgctg gactacctta caaagagata
    18361 caaacacggc tcggtcatgc aaaactttct atgaccatgg atatttatag ccacctatcc
    18421 aaagacaatc agaaaaatgc tacttcattt tatgaaaaag ctattgaaaa attaaaaagt
    18481 tcctaaaaaa gtccataaaa ttattatttc agttatggga aatagctata tcaaggaatt
    18541 ttagc
//
LOCUS       DAVAAL010000028        18501 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVAAL010000028 DAVAAL010000000
VERSION     DAVAAL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.029"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(136..1698)
                     /gene="guaA"
                     /locus_tag="VQM60_000804"
     CDS             complement(136..1698)
                     /gene="guaA"
                     /locus_tag="VQM60_000804"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET1080692.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1840..2538
                     /locus_tag="VQM60_000805"
     CDS             1840..2538
                     /locus_tag="VQM60_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET1080693.1"
                     /translation="MLPAYMKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPERFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2581..3477)
                     /locus_tag="VQM60_000806"
     CDS             complement(2581..3477)
                     /locus_tag="VQM60_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET1080694.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFTIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3486..4421)
                     /locus_tag="VQM60_000807"
     CDS             complement(3486..4421)
                     /locus_tag="VQM60_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET1080695.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLSQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4418..5143)
                     /locus_tag="VQM60_000808"
     CDS             complement(4418..5143)
                     /locus_tag="VQM60_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET1080696.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5227..5862)
                     /locus_tag="VQM60_000809"
     CDS             complement(5227..5862)
                     /locus_tag="VQM60_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET1080697.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKMIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     ETLNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(5864..6691)
                     /locus_tag="VQM60_000810"
     CDS             complement(5864..6691)
                     /locus_tag="VQM60_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET1080698.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6713..7048)
                     /locus_tag="VQM60_000811"
     CDS             complement(6713..7048)
                     /locus_tag="VQM60_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HET1080699.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEGGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(7049..7765)
                     /locus_tag="VQM60_000812"
     CDS             complement(7049..7765)
                     /locus_tag="VQM60_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET1080700.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISTADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNGIKAALLAYSYGFNGIEQYISKEDYNRYLSD
                     LNEDKMKAEIERAEKEADITIIMLQMGVEYQLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIIY"
     gene            8278..8889
                     /locus_tag="VQM60_000813"
     CDS             8278..8889
                     /locus_tag="VQM60_000813"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET1080701.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            8934..9662
                     /gene="rlmB"
                     /locus_tag="VQM60_000814"
     CDS             8934..9662
                     /gene="rlmB"
                     /locus_tag="VQM60_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET1080702.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9700..10611)
                     /gene="trxB"
                     /locus_tag="VQM60_000815"
     CDS             complement(9700..10611)
                     /gene="trxB"
                     /locus_tag="VQM60_000815"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET1080703.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQVFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10677..10901)
                     /locus_tag="VQM60_000816"
     CDS             complement(10677..10901)
                     /locus_tag="VQM60_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET1080704.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(10979..11722)
                     /locus_tag="VQM60_000817"
     CDS             complement(10979..11722)
                     /locus_tag="VQM60_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET1080705.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRRLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11722..12522)
                     /locus_tag="VQM60_000818"
     CDS             complement(11722..12522)
                     /locus_tag="VQM60_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET1080706.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12680..13036)
                     /locus_tag="VQM60_000819"
     CDS             complement(12680..13036)
                     /locus_tag="VQM60_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET1080707.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13030..13560)
                     /locus_tag="VQM60_000820"
     CDS             complement(13030..13560)
                     /locus_tag="VQM60_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET1080708.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(13560..14054)
                     /locus_tag="VQM60_000821"
     CDS             complement(13560..14054)
                     /locus_tag="VQM60_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1080709.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14189..14635
                     /locus_tag="VQM60_000822"
     CDS             14189..14635
                     /locus_tag="VQM60_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET1080710.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14632..15279
                     /locus_tag="VQM60_000823"
     CDS             14632..15279
                     /locus_tag="VQM60_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET1080711.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     NQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASVLQYIAIVYYM"
     gene            complement(15300..15881)
                     /gene="pdxT"
                     /locus_tag="VQM60_000824"
     CDS             complement(15300..15881)
                     /gene="pdxT"
                     /locus_tag="VQM60_000824"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET1080712.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15882..16757)
                     /gene="pdxS"
                     /locus_tag="VQM60_000825"
     CDS             complement(15882..16757)
                     /gene="pdxS"
                     /locus_tag="VQM60_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET1080713.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(16909..18288)
                     /locus_tag="VQM60_000826"
     CDS             complement(16909..18288)
                     /locus_tag="VQM60_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET1080714.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
BASE COUNT         5479 a         4216 c         3298 g         5508 t
ORIGIN      
        1 tttttgtcca aatgtaaacc acttggaact attattcaat ttattctttt tagtaaatgt
       61 tcccgtttta atcccagtta cagtttttaa tcctctgtaa ccgttgattt tacaaagctt
      121 ttaatttttt tgcgattact cccactcaac tgttgcaggt ggtttacttg taatatcgta
      181 gacgatacgg ttaacatgat ccacttcatt tacgatacgt actgagattt tttgaagtac
      241 ttcccatgga attttggcaa aatcagcagt cataccatcg atagaagtga tagcacggat
      301 tgcaatcgtg tagtcatacg tacgaccgtc acccataaca ccgactgaac gaacgcctgt
      361 gttaacagtg aagtattgcc aaatatcgcg gtcaagtcca gctttagcga tttcttcacg
      421 aagaatagcg tctgattcac gaacggtttc aagtttctct tcagtgattt cacccatgac
      481 acggatagca agtcctggtc ctgggaatgg ttggcgccat acgatatggt ctggcatacc
      541 aagctctgta ccaagagcac gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa
      601 ttcaaactgc atatcttctg gaagaccacc cacgttgtgg tgtgacttga tagtttgagc
      661 tgtatccgta ccagactcga taacatctgt atataaagta ccttgagcaa ggaatttcac
      721 atctttgagc ttgcttgctt cgtcatcgaa tacatagaca aactcgttac cgatgatttt
      781 acgtttttgt tcagggtcag aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc
      841 tgctttgacg atattcaaac caaacttacc accgagcatg tccataactt gatcagcttc
      901 gcctttacga agaagaccgt ggtctacgaa gatacagatc aattgatcgc caatcgcttt
      961 ttggagaaga accccaacga cagatgagtc aacaccacct gatagaccaa gaaggacacg
     1021 tttatcaccg acggtttcac gaattttttt gatctgcatg tcaatgaaat tatccattga
     1081 ccagtcacct ttagccttac aaatgttaag ggcaaagtta cgaaggatat catttccgta
     1141 tacagaatga cgaacttctg ggtggaattg gataccgtaa atgtgtttat ctgggttttc
     1201 gatggctgcg tatgggcagt cagctgatgt acctgtacga acaaagtcag caggaatctc
     1261 agtaaccgca tcaccatggc tcatcaaaac agtctgttca tcaggtgttg attcaaaaag
     1321 cgctgatggt gtgtgagtta gggttgattg accgtattca cgatttccag catcacctgc
     1381 aggaacaact tttcctccaa gtttatgggt caataactgc ataccataac agattcccaa
     1441 aattggaatt ccgagttcga agatttctgg gtcaatatca aatgaaccat cttcatatac
     1501 agaatttgga ccacctgata gaataattcc tacaggattg acttcacgaa cttcagcagc
     1561 tgaaatttta tggcttttta gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat
     1621 cagctggttg tactggctac catagtccaa tacgatgatt ttttctacat cttgcaaatc
     1681 agttgaaatg ttgctcatct ttttcctttc tcaaaagaaa tatcttttcc caatattcta
     1741 tcacaaataa gggcgaatta ccaactaaac aaggcaaagt ttgacttttt ctagtttatt
     1801 ggggtacaat agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc ttatatgaaa
     1861 atccatgatc agataaaaaa ggatattgac gagtaccgtt gggctattgg tgagaggctt
     1921 ccaagtgaaa gagatttagc tgagcagttt gcggtcagtc gtatgaccct ccgccaagcc
     1981 gtatctctat tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg cacctttgtt
     2041 tccagtactc gagtacaaga aaagatgcga gggacaacca gttttactga aattgtcaaa
     2101 tcccaaggta aagttccctc tagccagctc atttcctaca aaaaaaccat tcccaatgag
     2161 caagaagttg ccaagctagg aatttttcca acggacaata ttatccgaat ggagcgggtc
     2221 cgctatgccg accaagttcc cctagtttat gaagttgctt ctattcctga aagattcatt
     2281 aaggacttta aaaaagaaga aatcaccagt catttcttcc aaaccttgca aaaacatggt
     2341 tatcgtatcg gcaaatctca acagaccatc tatgctcgcc ttgctaaaga aaagattgcc
     2401 cactatttgg aagttgaaaa aggacatgct attcttggat tgacccaggt ttcctaccta
     2461 gaagatggta ctgcttttga atacgtaaaa agtcaatatg taggcgaacg ttttgaattt
     2521 tatcttgaaa ataattagat taccacgcaa aaactctctg tcacggacaa agagtctttt
     2581 ttatttttct aagaataaat ctttcaaaga aatcaaagtt acagcaaaca atatcattgc
     2641 cataccaaga aaatcaatgg gataaaattg ttcattcatt attaagaagg caaagaaaac
     2701 cgccgatatt ggctcaattg aagccaacaa gcttgacttg accggtccta tcagactggc
     2761 tcctttaagg aaagctgtat aggcaaagac agtcccgata aggataatgc ctgcaaacgc
     2821 aaggagaaaa tcaagactag tcgggatatc ggcctgtaga acccctgtaa aaggaagggc
     2881 gaccaaacct gctatgacca ttcccacacc aatgaccaag ctgctccccc actttttaat
     2941 caaggctatg ggtaaaatga tatacagagc ataagtcaag gcagaaaaga gaccccagaa
     3001 cagaccagca ggtgtcatgg ataactggtc caactgccca tgtgttgcga tcaggaaggt
     3061 tcctccgatg gtgaatatga tggaaactat ctctcccagt gtcggtgcca ccctatcctt
     3121 gatacagcta taaattaaaa ttccgacagg acaaacatac tgaagcaccg tcgctgttcc
     3181 cgcattggtc tcctgaatag cagacagata ggcgaattgg ttgagaaaaa gaccaatcag
     3241 agcaaaaata agaagagaca gcaaactctt tctatccttt aaaaagacca gtattttatc
     3301 ctttgcagta gcataagcca agagcatgag aattccacca gcgattaaaa gacgcaagtt
     3361 ggttaagacc agagccgaaa ttccgtgtgc catcaggtat tggccactcg ttcctgacaa
     3421 cccccaagca ataccagcca caactgttag taaagtccct tttaaactat ttgacatgtc
     3481 tctccttact cttctttgcg aatcaagttc atcacttgat tgcggtctaa tatccattga
     3541 gctctctcgt ccttttcata ggtacgattc gataggaaaa tagtggcttc ttgcttctga
     3601 cgattccaca tgataaaggt acctgtatag cccgtatggt ctagccaatc tccttccaaa
     3661 ttccatgcta aagaacgttc cttgtcatcc aaaggagaaa aattttgact taagtctctt
     3721 gcaaaatcat ctgctaaata gtgttctaaa aagatttgta aatcctttat agtcgaaaat
     3781 aaaccagcgc tcccagcatg tctacccagg agacgagcct tgggatcatg cactatgcct
     3841 gcctctacac ctctaactgt tggaacagca agctcaactg gcccaaactt ggtttccgtc
     3901 attccccaag gtttccagac ttgatccttt aaaatcacat ccaaatcttg attaaaaatt
     3961 ctttccaaaa taaagcccaa cagcaaaaaa tggacatccg aataaagaaa ggctggctga
     4021 cttcgtctgt tgagatgaaa catcgcttcc tttaattcag gggctgttaa aagatcacga
     4081 ttaggaataa aaggatcaag gtctgttgca tgagtcaaga gctggcgaat agtaatgtct
     4141 ggataatcac tctcaggtaa aaaatctatt accagtctat caatatctaa ttgacctatt
     4201 tcccacaaga aggtacaaac tgtgccaacc ccaacaacct tgctgacact agctaggtca
     4261 taaactagtc ctgcctcagt ctgcaagcca tgctctgggt cactctggcc taaatagaac
     4321 tctgtccatt gattgtcctt aaaatacgca aaagaggctc cgggataaat tcctgcctcg
     4381 atttgttctt ctattttttt aataatcttg gtccacttca tacttcttca aaccacaatt
     4441 caacattatt tctatcttta ccaaggaaga atttttcaga cttaggaata aaatattcag
     4501 tagactcaaa cttctggcga agacttgcta tgtctaattc attgaccaag aacttgagca
     4561 tagataagtc ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga gctggaataa
     4621 aatcaaggga tgccccaatt tcagatgatt ccaagaaact ttcgatatca gttgggagat
     4681 gtaattccat agaaatctca aatttactta aagaaattga tgccaaatct gtttgaaatt
     4741 caggcttttc tcctacttct actagacttg ctctgtcatc ttccgcatga atcaaaatca
     4801 aatcatcttc tggtgagaaa atttcaaaag cgtagccatt ttgaccttta tataatcgat
     4861 gaatcgaatc tgttttagat aagattcctt caatttctaa gggattttcc accttgacaa
     4921 tcaatctagc tagttttttt cttccctcta ccttacgagt acgcatactg ggagcttctt
     4981 ctaaaaccag cttttcaaga cccgtttggt cacctagtga cagaaaggcc gattcttcta
     5041 acaaggcctt cattccaagg gtttcaatat aaaatgtttc atttaatttt ctattattag
     5101 cttttaaagt aggaataatc cgtacaatct gatttacatt cataaattcc tccgtcactt
     5161 tttattttaa aggattttag aattttttac aagatattat gctcaaattt aacattattt
     5221 agctatttag taaagatagt ctttcaaggc ttttgacaaa gcctgataac cagcaatact
     5281 gaggtgcagt ccatcagttg tatattcttt tttgagttgg cctgcttggt ctgtcaaaca
     5341 atcaaatact ggcacaaatt ccacctgcat ataggcagat gcaagctctt gataggcttg
     5401 attccaattc tgaatttttt cattcgagcg gatatagact gcctgctggt actcctctcc
     5461 ctcattgaca ggcaaaatgg aaagcaattt aatctctgtc aatggataat cgcgagcaac
     5521 ggattgaatg atagcttcga gattattgag agtctcattc acaggaacat cctttccgat
     5581 atcatttgtc ccaatcagaa gaaaaatttt atctactgct ccaccatata gatgagcatc
     5641 aaggttctct agtaacagtc ctgtctgata gccacgaatt cctcgattga caatcatctt
     5701 tgaagttcca aatagctcct gtagaggata atactcgaca atggaatccc caataaaaag
     5761 aatgtttggt tctacaacag aaatgtgatt taggtgacga tatttggttt gaattttttc
     5821 ttgttccttt agaagccaat tttctaataa ctgtactgcc acttcattct cctttttcta
     5881 accagttttc caagacttga taaactcctc cttggctgtt ggctggtgct agagcagtcg
     5941 ccacagcttt ggctttctca tcagcatttt ccatcgcata ggcaattcca gccatttcaa
     6001 gcatttcaac atcattttca ctatcaccaa aagccatgat ttcttgggat ttcaagtccc
     6061 agcgcttgag taattcctcc aagccccatg ctttatgaat cccagcttgg aggatatcaa
     6121 tgcaaccata gccactggat acagctcgga cacggccatc aaagagagca ttgatttctt
     6181 ccaaaaccga actcaaacgt tcctcaccaa caaccatgct catcttgagc acaccaccaa
     6241 agaggtcaga tgttaattca tccacaaatt gcatccgttg gtagaatttt tcaatcattt
     6301 ctggagtcat aaaactttca agatctgtaa aaatcgtacc ttccttgaca aaatcaccct
     6361 tcatccccgt tacaacaaac tggtcctgac acgctcgacc cttgaaatga gccaaagcct
     6421 tgttgacaat ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt tcaaaaatac
     6481 gagcgccatt agcaacaacc agaaccactc gatccaccaa gggactcagt agttgtctca
     6541 tgcggtgaat ttcattgccc gtcgcaatga caaaacgaat gcccctttga tccaactgat
     6601 ctaaaatctt ttccaatcgt gggagatcaa gctgacctct agcatccagc aaggtcccat
     6661 ccatatctgt cgcaattacc ttaatcatca tttactttcc tctggattca agctaatacc
     6721 acttcaatcc cacatgttca ttcatttctt tataggctgt atcaattcgt tccttagtcg
     6781 cttcatctaa ctggtcacga tgactgccac cctctataaa atcttccaaa atataagttt
     6841 ggtaatgata caagggatat ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc
     6901 aagtaggatg agctttagct gttccgatag ttgtttttcc atccttcttc ttgatggtga
     6961 catccatgag aacaccacgt tcagtccact tagcattctc ttcatctccc atagattcaa
     7021 tccgttgatt ggaaatgaag ttccccattt aatagataat gagcttctta tctccatctt
     7081 tttcaaccgt ttcagatggt tcaacaacgt gaggatgccc tccaaagata atatccgctc
     7141 cccaatcgat catcttgtga taaagagctt tttgttcttc agttggttcc aattgatact
     7201 caacacccat ctgaagcatg ataatggtga tatctgcttc cttctctgcc cgttcaattt
     7261 cagccttcat cttatcttcg tttaaatctg aaagataacg attatagtct tccttagaaa
     7321 tatactgctc aattccattg aaaccatagg aataggctaa caatgcagcc ttgataccat
     7381 tcacttcctt aatgaccagc ggagcctgat cacgtggttc gtgcgtataa actccgattg
     7441 gagtgattcc agctttctca ataatatcgg ccgttgaaat aactccctca atttgcgaat
     7501 ccaaaatatg attatgagct aaatccagca catgataacc tgcctcctta atagcatcca
     7561 taacttcaac aggagcatta aagagaagat aacccgctaa ataatgatcc ttattaatgg
     7621 ttccttcaaa atcaccaata gctaaatctg cttgcttgag ccaaggagtc acatactcaa
     7681 aattttcatg aaagtcatag gtaccgtctt cttttttagc tgaaaagaaa agtccatcgt
     7741 ggtagagcaa atccccatgg gccataattc tggcagtttt ttcctcttcc tactcctgag
     7801 acttttgctt agtcccctct tgagaaatag tatctcgttt ttgactagtc aagggattcc
     7861 ttggaagctt tcaaacaaca agatcaagcc cattgataaa ccaactgcta gcaagagtat
     7921 cgccacaaat cccttattgc tccacttgcg ataactccta aaaagtttac caagcccttc
     7981 ataaaacgaa aagctaaacc accctgattt cgattttgtc ttctttgtat cttcgttctc
     8041 cctactttct tatgcaagcc ttttcttttt attatatcac agataagtat ttctttcaca
     8101 attgaattga acttcccatc tattttctat aaatcctaaa tgccataatg ctttcaattc
     8161 ttgtcatttt gtgatatcat gtagaggaaa tgaactaatc cacagtggct tattccaagt
     8221 ataccacttg ggctttggca gtaactaact gcactaaaca taatataagg agagaaaatg
     8281 tctgcaatag aacgtattac aaaagctgct cacttaattg atatgaacga tattatccgt
     8341 gaagggaatc ctactctacg cgcgattgct gaggaagtca ctttccccct atctgaccag
     8401 gaaatcatcc taggcgaaaa gatgatgcaa ttccttaaac attcccaaga tcctgtcatg
     8461 gctgaaaaaa tgggactccg cggtggtgtt ggactggctg ctccccagtt agatatctca
     8521 aaacgcatta tcgctgtttt ggtacctaat attgttgaag aaggcgaaac tccacaggaa
     8581 gcctacgatt tggaagccat tatgtacaat ccaaaaatcg tctctcactc tgttcaagat
     8641 gctgctcttg gcgaaggaga aggttgcctg tctgttgacc gtaacgtgcc tggctatgtt
     8701 gttcgccatg cccgcgttac tgttgactac tttgacaaag atggagaaaa acaccgtatc
     8761 aaactcaaag gctacaactc cattgttgtt cagcatgaaa ttgaccacat taacggtatc
     8821 atgttttacg atcgcatcaa tgaaaaagac ccatttgcag ttaaagatgg tttactgatt
     8881 cttgaataaa gaaaatcccg ttgcaagacg gggttttgtg ttataataga ggcatgaaaa
     8941 caaatgatat tgtctatggc gtccacgccg ttaccgaagc cctccttgca aatacaggaa
     9001 acaaactcta cctccaagaa gatctccgag gtaagaatgt tgagaaagtc aaggaactag
     9061 ctacagaaaa gaaggtgtcc atttcttgga catcaaaaaa atctctctct gagattactg
     9121 aaggtgctgt tcatcaaggt tttgttctac gagtgtctga atttgcctat agcgagctag
     9181 attacatcct tgcaaaaaca cgccaagaag aaaatccact tctattgatt ctagatggtc
     9241 taaccgatcc ccataatcta ggttctatct tgcgaacagc cgatgcgacc aatgtttcag
     9301 gtgtcatcat tcccaagcac cgtgctgtcg gagtaactcc tgtcgttgcc aaaacagcca
     9361 caggtgctat tgaacacgtt ccaattgccc gagtgaccaa cctcagtcaa accttagata
     9421 aacttaagga tgaaggtttc tggacctttg gaacggatat gaacggtact ccttgccaca
     9481 agtggaatac aaaagggaaa atcgccctca tcattggaaa tgaaggaaaa ggtatctcta
     9541 gcaacatcaa aaaacaagtc gatgagatga ttaccattcc gatgaatgga catgttcaaa
     9601 gccttaatgc cagtgttgct gcggccattc tcatgtacga agttttccga aatagactat
     9661 aaaaagtttc cagtcatctg attggaaact tttttataat taactatgtt ctgtaatgaa
     9721 tttataggct tcttgaccag cgatagctcc atctccaacc gctgttgtta cttggcgaag
     9781 gtctttcaag cgaacatctc caactgcaaa gataccgtcg actgcagttt tcatgtggtt
     9841 atctgtcaca atccatcctg cctgatcttg gatattcaat tctttaacaa aatcgctaag
     9901 agggtccaaa ccaacataga taaagacacc accgaagact tgttctgtca cttgacctgt
     9961 tttcacattt tcaaatacga ctgattctac tcggttttca cccttgattt cccttactac
    10021 agaatcccag ataaagctga ttttttcatt cgcaaaggcg cgatcttgta aaaccttttg
    10081 ggcacgaagt tggtcacgac ggtgaacaat ggtaacagtc ttagcaaaac gagtcaagaa
    10141 gagggcttct tcaacagctg aatctccacc accaactacc aataaatctt ggtcacggaa
    10201 gaaagcacca tcacacacag cacagtaaga aacaccacga ctgttcagtt cttcttctcc
    10261 aggtactccc aaaggacggt gtttagaacc agttgctacg ataactgtac gtgtttcata
    10321 tgtttggtca tcagtcatca ctttcttaaa atcaccatgg tcttcgacat tttcaacata
    10381 accataaatg tgctccacac caagattttc aagtggttca aacatctttt cagccaattc
    10441 aggtccacta atattagcgt atcctgggta attttcgata tcagatgtat tattcatctg
    10501 accacctggc agaccacctt caatcaaagc tacttttaga ttgcttcgag cagcatacaa
    10561 ggccgcagtc atccctgcag gtccagcacc gataataata gtatcgtaca tatagattcc
    10621 ttctttcttg ttgtaactat ctttattcta actctttctt gtcaatcacg caaggattat
    10681 gccttgaaaa caagaattaa actcataacc gcaaaggata atactgggac aaccaagact
    10741 ccaatcataa tcatatcata gagatgggct tgacgagccc tggctgtctt atcaactccc
    10801 gacatggctc tcagaccaat ccaaatccct aaaaaaatca gaacaaggat ggtggtcaag
    10861 atcaaactct cgaaatataa agaaaatagt tgcagtagca tgatttctct catttctatc
    10921 ttttttaaag agtaaactca gctagtccaa ctaactgagt tttcctttat ctattatatc
    10981 aaatataagt ccgtttgtaa ctagcgaaga attcttttgt ccgctcttct ttaggggtgt
    11041 ggataatctc atccggagtt ccagactcga tgattttccc cttatctaag aagagaattt
    11101 tatccgcaac ttgggctaca aaggacatgt catgactgac caaaatcatg gtctgacctg
    11161 acttagcagc atctgcaata gacttttcta cttcaccgac caattctggg tcaagggctg
    11221 aagttggttc gtctaagagc aaaacatctg gtttcatagc aagcgcacgc gctagggcaa
    11281 cccgttgctt ctgtccacct gataaacggc gaggataatg gttttcacgg tccgaaagcc
    11341 caaccttagc caactcttcc ttggcaatct tagtcgcttc ttggtcagat aatttcttga
    11401 caacaaccaa gccttctttc acattatcaa gtgctgttcg gcgttcaaac aaattaaact
    11461 gttggaaaac catagacaac ttacggcgta gggcaaggat ttcttcttga gtgattttag
    11521 aaaaatcaac tgaaaaacca tcaatctgaa tagagccact gtcaggtgtt tcaagataat
    11581 tgagactgcg aagaaaggtt gattttccag ctcctgaaga accaatcaag gctacgactt
    11641 ctcctttttg aatatccaag ttcagatgat ccaagacagt ctgtcctgaa aaggatttgc
    11701 ttaaattcga aatcttaatc attaacgaag gtctcctttc acatctgttt gcactgtatc
    11761 aggtgcagaa atagccattt ttctctcgat gaaacgaccg aggctttcaa ttccgatatt
    11821 gactacccaa taaacaaggg caacggagat gaagcgttca aaatagcgat aatcagctcc
    11881 acctagaatc tgagcttggg caaagacttc cacaacaccc gcactaaaag ctagagatgt
    11941 tcccttggtc aaaccgatga gggaattaat caaggttgga gtagctacca ccgctgcatt
    12001 aggaataatc actcgtcgat aaacttgcgc tcgggtcata cccagactgc gtgccgcctc
    12061 aatctcacca ggattaactg agagaatggc tgcacgaatg gtttcactag cataagctgc
    12121 ctcattaaag gcaaaggcga caatcgcaaa agctgcagct ggaatcgcat tgatattgag
    12181 accagttccc cattgctgat tgagggcttt caaagccaaa ggaattccgt agtaggtcaa
    12241 catgagttgc accaaaatcg gtgtcccttt taagaaacta acaaagaagg cctgcaaggg
    12301 atataaaatc ttgacacgat tgatcttcac aatggcaaaa agaagcgcca aaaccaagcc
    12361 aaaaagggca ccgccaattg tcaacataat tgttgttgga agttgttgga caattctagg
    12421 gattccatca aagaccgaac gtaggctaaa cagcttgcca tccggaatca attgcatcaa
    12481 gttttggtac caatctgatg ctaaaatcgt tgtaacattc ataaaaacat cctcttctga
    12541 taatgattca ttctaccatg cttctgccgt caatgaaatt atttgctacg gtcagataca
    12601 gactttgtct acctactagt ttatcatact ttgaaacagg gaagttatat attttatcta
    12661 tcaaagagat agataaaaac tatttcagtc ccagttcttc ataagatttt cgataaccga
    12721 tttgcttaac agttccattt tctactaaaa tgggccgttt taacaacata ccgtcacttg
    12781 ctagcaactc agccgcttct tggtttgaca aacttcctac cttatctttt agccctaatt
    12841 cacggtattt gataccactg gtgttgaaaa attgcttcaa ttcaaatcct gaggtttcta
    12901 gccaattcaa aatgacttct tggctaggtg tttcttccac gatatggacg gctttatagt
    12961 ccacacctaa ttgatttaat tcttgttttg cttttttaca agttgaacat ttggggtatt
    13021 cgataaattc taacatgtgc ttctccttct attgtttcta tctttaaaat ctactccttc
    13081 atgctccaag agccaagctt tcttttccac tcctgcagca taacctgtca gacgcttgcc
    13141 tgctcccaac acacgatgac aaggtactag gatagaccaa ggattgcgtc ccactgctcc
    13201 accaattgct tgagcagaag ccacttgcag gtcttgagca atttgtccat aggtcactgt
    13261 ctgaccataa ggaatgccct gtaaatagtc ccaaactctc ttttcaaaat tcgttccgat
    13321 tggcgccaag agcaagttgg ataaatcctg aggcttgcct ttaaagtaat catctaagca
    13381 agcaataact gggtctaaaa taggatgact aacaacttct tctatcgttt catctcctag
    13441 tcccctctca aaatgcttct gctcctgaac ccaaattcca tacaaataat ggtcatcagc
    13501 tacaagtgat agaataccaa ttggtgagga gtagaggatt tttacatact tcttttgcat
    13561 tatttcttta atttttgata ggccaagcgt tcgccatcta ctggcacctt accaacttgt
    13621 ttaaaaccaa gtttttcaaa aatatgttgc ataaccttgt tttcagcatg cgtatctgag
    13681 cgaaaatcaa gataatcaaa accttcaatc aagccctcta agaaggtttg agcaactcct
    13741 tttccctgca catctgctgc cacagcaata cggtgaaaga ctagatactc tgactctccc
    13801 gcttgccagt ttccctcata aatagcttca taggcctcct ctggactctt ggtcacagca
    13861 gcataggcta gcagttctcc ctcttccaag gctacgtagg cttgccctga gatgatatca
    13921 tcgataataa catctgcatt tggatagcca ttttgccact ggtcactacc agcatctgct
    13981 aaacattttt tggcatcctc catcacctgc atgatggcat ctacttcatt tggaaaagct
    14041 aaacgaattt ccatcttttt tcctcatttc tgactagttt ctcccatcat agcataattt
    14101 aagtatttct acaagcagtt aaaacttgac tttgtgtttt tgttatttta taatctagtt
    14161 atcaaaacta tataaaaagg agttgaaaat gaaaactacc ttttcctacc caaaatgggc
    14221 agaaattcca aacattgacc tctatctgga ccaggttttg ctctatgtca atcaggtctg
    14281 cgcccctatc tctcctaata aagacaaggg cctaacagca tctatggtca acaattatgt
    14341 gaaaaatggt tacctgacaa agcctgacaa aaaaaaatac caacgccaac agattgcccg
    14401 tttgattgct atcacaaccc tcaagtctgt attttctatt caagaaatag cccagacact
    14461 taatactcta caaactcaag caagttcaga ccaactctac gatgcttttg tggactacat
    14521 gaaccaagga attgacccag ctaaccctat tatccaaacc agctgccaaa ccgttaaact
    14581 ctatcatcaa actctagact taatcgatca tactcaagag gaggtaatcc aatgaacact
    14641 agtcttaaac tcagcaaaca actcagtttt ggagaggaga ttgctaatag cgtgacccat
    14701 gctgtgggtg cagtcatcat gcttatcttg ctgcctattt catccatcta tagttatgaa
    14761 gcacacggat ttttatcctc tatcggcgtt tccattttcg tcatcagtct ctttctcatg
    14821 ttcctatcat ccaccattta tcactctatg gcctatggtt cgacccacaa atatgttttg
    14881 cgaatcattg accattctat gatttacgtt gccattgccg gctcatacac gcccgttgtc
    14941 ttgaccttga tgaataactg gtttggctat ctgattattg tcatccaatg gggaacgacc
    15001 atctttggta ttctctataa aatctttgct aaaaaggtca atgagaaatt tagccttgct
    15061 ctttacctga ttatgggctg gttggttctg gctatcattc ctgccattat caatcaaacg
    15121 acacccgttt tctggagtct catggtaact ggcggactct gttatacagt tggagctgga
    15181 ttttatgcca agaaaaaacc ttatttccac atgatttggc atctctttat cctagctgcg
    15241 tccgtactcc aatacatcgc tattgtttat tacatgtaaa aaagttgaga aattcaatct
    15301 caactttttt ctttacacat attgataaag tactggtgca agcgcacatc atcagtcaat
    15361 tctggatgaa aagaacttac caacatattt ttttcttggg ctgcaacaat ttgattgttc
    15421 actgttgcta gaatttctac accctcacca acactactga taatcggacc acggataaag
    15481 gtcattggaa tcttgccaac tcccttacat tctgcttccg tgtagaaact tcctaattgg
    15541 cgcccataag cattacgctc gaccaccata tccatagttc ctagatgact ctctttctga
    15601 gaagtgattt ccttagccag caaaattaag cccgcacagg tcccaaacac tggtaagcca
    15661 gatagaatgg cttctcggat gggaagtagc atgttctggt cacgtaagag cttgcccatg
    15721 gttgtagact caccaccagg caaaatcaaa cccgacaagt cactctgatc ttgctgaaaa
    15781 tcatctagat ttctgagttc tacactctcg acacctaatt gatctagcac ttttgcatgt
    15841 tctgcaaagg ccccttgcaa ggccaatatt ccgattttca tctattttcc tcgttcagcc
    15901 atgaggattt ggatttcatt ttcattaata ccaaccatgg cttctcctaa atcttcagag
    15961 atttgagcta ggatttgagg attacggaag ttagtcacag ccttaacaat ggcactcgct
    16021 cgtttaacag gatctcctga cttgaaaata cctgaaccga caaagacccc ctctgcccct
    16081 agttgcatca ttaacgcagc atctgctggc gttgcaacac ctccagcagc gaaatttaca
    16141 actggcaatt ttccatgttc atgaacatat tggaccaatt ctacagggac ttgcaaatcc
    16201 ttggcagcaa cataaagctc gtcctcacgt aagttttgaa tgcggcgaat ttcctgattc
    16261 atcatacgca tatgacgaac agcttggact atatcccctg tccctggttc tcctttggta
    16321 cgaatcatgg aagcaccttc agcgatacga cgcaaggctt cacccaaatc cttagcacca
    16381 cagacaaaag gaacttggaa ttctttcttg tccacatgga aacggtcatc agctggagat
    16441 agaacttcac tctcgtcgat ataatcaatt tcaatagcct ctaaaatctg agcttcaaca
    16501 aaatgcccga ttctgacctt agccattact ggaatactaa ccgcttcttg gatttcctta
    16561 atcatctttg ggtcgctcat gcgggaaact cctccagctg cacgaatatc agccggaatt
    16621 cgttccaagg ccatcacagc tgccgcacca gcagcttctg cgatacgagc ctgttcagga
    16681 ttctgcacat ccataataac accacccttg agcatctgtg ccaagttttt atttagttca
    16741 taacgatttt cagtcatttg ttttatcctc atcatttgta ttggtagcta ccttttcatt
    16801 ttaagtcaga acagaatgaa gcacaagata aaaaaactat aattgtatgg ggacaaatta
    16861 gctaaaaact atctgacctt aacttaaggc cagatagctc attcattttt atttttcagc
    16921 cgtaagggca gccattgtga tgtagttgta tggtttgttg aagtgtggca agaagaagag
    16981 gtctgtcaat gccaatttat caattgtcac atgctcttgg atagcaagtg agaacatgtg
    17041 gattcccatg ctaattgcaa tatcatgtga aaccatttgg gcaccaagaa tttcacggct
    17101 atctttgtca aagacaatct taattgctac ttcatggttg tcatgtttca tgaattctgg
    17161 tttttgaaga tcgttaaagc ctgtttcagt tgcgttgtaa ccagcagctt tcgctttttc
    17221 aagagtcaaa ccagttgaaa ccatgtgaag accgtagatt gagataccat ttgaaccttg
    17281 aacaccgatt ccttccaatt catgtccaca agcattgtag gcaccaacga taccagtgcg
    17341 aacagcattt gaagcaagag cgatatagct tgtatcttta cgagcattgt cataaacagt
    17401 cgcacagtca ccaacagcgt aaacgcctgg gatagatgtt tcttgtttct tgtctacaag
    17461 gaaggcaccg ttgcggaaga gttcgatctt accatctgca agggctgtgt ttggacggaa
    17521 accaactgca aggataacca tatccacgtc aaagctttct ttgtcagtaa tcaagcgttc
    17581 aactttaccg tcaccttcga ttgctttaac agtttgacct agagccaagc ggatgttgtg
    17641 atcttccaag ttcttcgcca tcatttgtgt gaagtctttg tcatagtaac cgttcaagac
    17701 agtatcaacg atatcaacaa ggacaacttc ttttccaaga cgttcaaagg cttcagcaag
    17761 ttcaacaccg atgtaaccac caccaacaac ggcgatacgg tcgaggtgtt ggctcttgtc
    17821 agaaagttta ttgataactt cttcagcatt ttggtacaat ttcacgaatt gtacgttttc
    17881 aagagttgct ttaaattcgc ggtttccttt aacaatttca acaccttcga ttggtggcaa
    17941 gattggtgta gaacctgtag cgaaaatcaa tttttcgtat gattctttgt gctcttttcc
    18001 ttcaacttcc gctgtaacta ctttgttatc atagtcgatt gaaagaacag gtgagttcat
    18061 gtaaacttta gcacctttag cttccaattt ttctttatca gaatagaaca agccttcagc
    18121 accgtcaatt tgttcaccaa tccaaagagc cattccacat cctaggaaag agatgttaga
    18181 gttttggtca aatacaacaa tttcgttctc atttccaaaa ttatccaaca tggtattgat
    18241 acatgctgta ccagcgtggt tagcaccgac tacaacgatt ttactcataa aaatttcctg
    18301 ccttcattct aaatttacct tactagtata ccatttaccg ttagcgttta caaggggggt
    18361 actattattt cagcattttt tatcaaaaat aattccccat ttatcctgtt ttcaagcctg
    18421 atgaatttta tgagcatatt tcttgacttt ttccaaattt tgtttgtgaa aattctaact
    18481 ttatggtatt ataataacat g
//
LOCUS       DAVAAL010000029        18212 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVAAL010000029 DAVAAL010000000
VERSION     DAVAAL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18212
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.030"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(87..608)
                     /locus_tag="VQM60_000827"
     CDS             complement(87..608)
                     /locus_tag="VQM60_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET1080715.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDKK"
     gene            complement(580..1671)
                     /locus_tag="VQM60_000828"
     CDS             complement(580..1671)
                     /locus_tag="VQM60_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET1080716.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTIITVRMLRKSGSQNSNLY"
     gene            complement(1782..2036)
                     /locus_tag="VQM60_000829"
     CDS             complement(1782..2036)
                     /locus_tag="VQM60_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080717.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(2376..2909)
                     /locus_tag="VQM60_000830"
     CDS             complement(2376..2909)
                     /locus_tag="VQM60_000830"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET1080718.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLERFFKTR"
     gene            complement(2896..3111)
                     /locus_tag="VQM60_000831"
     CDS             complement(2896..3111)
                     /locus_tag="VQM60_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET1080719.1"
                     /translation="MGKERCEQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(3078..3221)
                     /locus_tag="VQM60_000832"
     CDS             complement(3078..3221)
                     /locus_tag="VQM60_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HET1080720.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            3455..5173
                     /locus_tag="VQM60_000833"
     CDS             3455..5173
                     /locus_tag="VQM60_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET1080721.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            5284..5631
                     /locus_tag="VQM60_000834"
     CDS             5284..5631
                     /locus_tag="VQM60_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET1080722.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(5910..6746)
                     /locus_tag="VQM60_000835"
     CDS             complement(5910..6746)
                     /locus_tag="VQM60_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET1080723.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGSRKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(6759..7388)
                     /locus_tag="VQM60_000836"
     CDS             complement(6759..7388)
                     /locus_tag="VQM60_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET1080724.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(7398..8039)
                     /locus_tag="VQM60_000837"
     CDS             complement(7398..8039)
                     /locus_tag="VQM60_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET1080725.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(8185..9414)
                     /locus_tag="VQM60_000838"
     CDS             complement(8185..9414)
                     /locus_tag="VQM60_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET1080726.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALHIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(9404..10570)
                     /locus_tag="VQM60_000839"
     CDS             complement(9404..10570)
                     /locus_tag="VQM60_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET1080727.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            10714..11727
                     /locus_tag="VQM60_000840"
     CDS             10714..11727
                     /locus_tag="VQM60_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET1080728.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(11772..12191)
                     /locus_tag="VQM60_000841"
     CDS             complement(11772..12191)
                     /locus_tag="VQM60_000841"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET1080729.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(12202..13608)
                     /gene="atpD"
                     /locus_tag="VQM60_000842"
     CDS             complement(12202..13608)
                     /gene="atpD"
                     /locus_tag="VQM60_000842"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET1080730.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTISMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(13694..14572)
                     /locus_tag="VQM60_000843"
     CDS             complement(13694..14572)
                     /locus_tag="VQM60_000843"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET1080731.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(14588..16093)
                     /locus_tag="VQM60_000844"
     CDS             complement(14588..16093)
                     /locus_tag="VQM60_000844"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET1080732.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(16108..16644)
                     /locus_tag="VQM60_000845"
     CDS             complement(16108..16644)
                     /locus_tag="VQM60_000845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET1080733.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(16644..17138)
                     /gene="atpF"
                     /locus_tag="VQM60_000846"
     CDS             complement(16644..17138)
                     /gene="atpF"
                     /locus_tag="VQM60_000846"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET1080734.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(17152..17868)
                     /gene="atpB"
                     /locus_tag="VQM60_000847"
     CDS             complement(17152..17868)
                     /gene="atpB"
                     /locus_tag="VQM60_000847"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET1080735.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(17903..18103)
                     /locus_tag="VQM60_000848"
     CDS             complement(17903..18103)
                     /locus_tag="VQM60_000848"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET1080736.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         5429 a         3986 c         3261 g         5536 t
ORIGIN      
        1 ttattcttca cgaaagacct cattctcaaa accaggaaaa agcccacatc tgtgatcttt
       61 tccttgttcc tagattctaa aatctattat tttttatctt cttttttctt gaagaatcct
      121 aatcctagac cagctaacaa ggtcatgata ccagcactag ccaaggtagc attagcttct
      181 gtacctgtgt ttggcaattc ctctctctta cctgtctcat aagtcggaac ttctgggtct
      241 ggattcactg gagtttcagt ttttggagta cctggttctg gagttggttt atctggtgtt
      301 gataaacggt cataccttac cgttatttct ttatcactag agtctgacgt aacttcttgt
      361 gattcaactg ttggaatatc tggatctttg tacttgtcaa tcttaccaga tataacctcg
      421 tcccagtttc ctgttgtcca ttcaccgtag gttacaactc ccgtgacctt gttctcagtt
      481 tttgtacggc ttaaggttac aggttgaaca acatcttctt ttacattttg gttcgtaact
      541 ttatcaacgt aatgaatgat acgcgttata gtcttcgtct cagtagaggt tgctgttttg
      601 ggaaccactt ttcctcaaca ttctcacggt aataatagtc aactgttgca ccgtcttctg
      661 gtacgcattt gcaggagttg ctaccaaggt gtatgttgtt ttccttgtga taactcggtc
      721 ttctttgtcc tcagttgttg ttttcccttc aatagttttt gattctgtgg tatactcaga
      781 acctatcgct aaatcagctt ttataacaga ctctgccaac ttctcttggc taccttcttt
      841 atagtaattg gctgttactg tagcagtggt tggcgcttcg ctttctctat aaactaaggt
      901 cactgttcta ccttcgctta caatattccc agttaaactt gcagaatttg tatctgcttc
      961 tttaaaagta taatattttc cgtcagtagt agtcatgcta ctgagttttt tatctgtgac
     1021 attgtagctg gtaccaatca gttgtttttt attggtaatg taggttccgt cactttcttt
     1081 ttctccaatt ccagtatcat ttttcaatga tagcaaacgc ccttgttcat caacatagcg
     1141 aactttcaca ttttctgaga ttagttctgc caattctgag gtttttttct ttttcttgat
     1201 ttcttcggtt attttccctt tctcttcttc gggaatattt agttttggaa tgattttttc
     1261 aacaacggtt cgtgatggtt ccacagtatc ttggatgact gaaaagtcag ctagaattgg
     1321 gagattatac tgaacacggt gactttgagt gtttactcct actctttcat tattctctga
     1381 aaatacttgt acggtataag aaacaacatc ttttcctaat agaacatccc cagtagagaa
     1441 atagccgcct tttcctagtt tgctatctcc agagcccact tctttcctaa tcttatcaga
     1501 tagtttttta ccagtcagta cattcgttcg cacaatccct ttgtctaccc ctacaaagtg
     1561 ggagaacttt ttgaactctt cagaaccaga tctagcccaa ccattattaa gggcatttgc
     1621 ttttgtattt gtattctctc tcaaaagttt ggcgattaga attatattca tcggcactta
     1681 gagttgctgc tatatctgac tcttgaatac caacttcctt actaccattt ctagcggcag
     1741 tatatgtgta tttatttgtt tctacttcta gctgattatc cttaacatga taggtaattt
     1801 tctcctggta taagagtctt cagtagttta tatgctctgt catcatcttt agctttagaa
     1861 gcagctgagt ttagccaagt tggggtatta ggagtatctt tatcagcctg tttcaagaca
     1921 ttattcaagt catttgctag cctattggtt gtcctcactg tgaaagtttt cttccagtct
     1981 gaaccacttt tattagactt agatatgctc atgaaatcct ggtcaacatt caacacaata
     2041 tcgccttgtt ttttagcatc ttccgttgct tctgcttcaa attgatcatg gaatttttgt
     2101 tgctctggtg tatagttttt ttatttacac gatttaatgt actatttttt attttagtat
     2161 taagaactcc attaatacga tttttaaact ctgcttcaaa aaatccctta atttgttggt
     2221 gcgcaagttc aacattcaaa cctttcttat acaagtcttc attctgaacg atttcggcta
     2281 cacggtgaat ggtttgatcg tataccgtct tctcttaacc agcagtatcc ttagttttaa
     2341 tgactactcg ataagttcct ttagaaccgt tatagtcatc tagttttaaa aaagcgttcc
     2401 aaacattcaa aaatagttgg aacttccctt aataaatccg tcactttctt tttcaagata
     2461 gcccaagctt gctcaatagg attcaaatcc ggtgaataag gtggtagagg taagaaaaag
     2521 tgtttatctt ggatgcagag ttcatccaaa atgttcttgg gatgaaaact agcattgccc
     2581 atgacaataa catgaggtgt cttcaaagca ggtaggagtt gcgttttgaa ccactccaca
     2641 aagaaatcgc ttgtcatgct tttcttgtaa atcatgggag ctataaactc tccgtctact
     2701 tgtcctgcaa caattgaagt tcgctcaaaa cgacgtccgc taatcttttc atagactttc
     2761 tcccctctag gagcccctgc ataaggacga tagaggtagc ggtcgattcc tgtttcgtca
     2821 atatatacga ttggtaaatc ttttaggtta tccaaaatat caagaaactc agcgactttc
     2881 tctggctctt gttccttaaa gctcgtcatc tttttttaaa gtgaccttaa tctgctttaa
     2941 agctgcccat actgaaggaa cagcacaatc aaaatgtgcc gcaatttccc gtaaaaaagc
     3001 atctggatga gcctctacaa aggctttcaa ttcctctaaa ggaatctttc ggtttttgac
     3061 gactcgcttt ttcctctcta tgtgtccttg ttcacatctt tcttttccca cgtgaagaga
     3121 gttctgacgc caacaccaaa aaacttggct gcctcgacat ggctgtgccc ctctttgatg
     3181 taatctaatg ctcgctgttt aaaatcagta ctatatgcca tagctttatt ataatatgct
     3241 tgtttaaaac taagcaacta tatcaatgtt ttgttattag aagacttact ttttttactg
     3301 aaatcgaatt tttcctaacc tcaacatcat ataaccccaa aattcaatct gacctggttt
     3361 gaagagatgt tcgaagcaga taaggtttta ctaaaactaa aaaaaatgat ataatcaaac
     3421 tgattaaatg ttttaaaaat aaaggagaat ttgaatgtct taccaagaaa attaccagaa
     3481 atgggttgat tttgtggagc ttcctgacta ccttcgtcaa gatttggaaa atatggacga
     3541 aaaaactaag gaagatgcct tctatacaaa tcttgaattt ggtactgcag gtatgcgtgg
     3601 cttggttggt gctggtacaa accgtatcaa catctacgtt gttcgccaag ctactgaagg
     3661 attggctcgt ttgattgagt caaaaggtgg aaacgagaaa gaacgcggtg tagcaattgc
     3721 ctacgatagc cgtcacttct cacctgagtt tgcctttgaa tctgcggcag ttcttgctaa
     3781 acacggcatc aaatcttacg tatttgaaag ccttcgtccg actccagaac tatcatttgc
     3841 agttcgtcat ctcaactgtt tcgcaggtat catggtcaca gccagccaca accctgctcc
     3901 atttaacggt tacaaggttt acggtgaaga cggtggacaa atgcctccac acgatgcgga
     3961 cgctttgact acttatatcc gtgcaatcga aaacccattt gcagttgaag ttgctgatgt
     4021 ggaaactgaa aaagcttctg gcttgattga agttatcggc gaagctgttg acgtagaata
     4081 ccttaaagag gttaaggacg taaacatcaa cccagccttg attgaagaat ttggtaaaga
     4141 catgaagatt gtctacacac cacttcatgg tactggtgaa atgttggctc gtcgtgctct
     4201 tgcccaagca ggatttgact ctgttcaagt tgttgaagcg caagcaactg ctgacccaga
     4261 cttctcaact gtaacatctc caaacccaga aagccaagca gcctttgccc ttgctgaaga
     4321 acttggtcgt caagttggtg cagatgttct tgtcgcaact gacccagacg ctgaccgtgt
     4381 tggtgttgaa gttcttcaaa aagatggtag ctacctcaac ctttcaggta accaaatcgg
     4441 tgctatcatg gctaaataca tcttggaagc tcacaaaaac gctggaactc ttcctgaaaa
     4501 tgccgctctc tgcaaatcta tcgtttcaac tgacttggta acgaagattg ctgaaagcta
     4561 cggcgcaact atgttcaacg tcttgacagg tttcaaattt atcgctgaaa aaatccaaga
     4621 attcgaagaa aaacacaacc acacttacat gatgggattt gaagaaagct tcggttactt
     4681 gattaaacca tttgtacgtg ataaagatgc catccaagcc gttcttgtcg ttgctgaact
     4741 tgctgcctac taccgttctc gtggtttgac acttgctgac ggtatcgaag aaatctataa
     4801 agaatatggc tactacgcag aaaaaacaat ctctgttact ctttcaggtg tcgatggtgc
     4861 tgaacaaatc aaagcgatta tggctaaatt ccgcaacaat gctccaacag aatggaacgc
     4921 aacagctatc actgtcgtag aagacttcaa ggcacaaact gctactgttg ctgacggtac
     4981 tgttacaaac ttgacaactc ctccaagtga tgtgttgaaa tacacacttg ctgacggttc
     5041 atggattgcc gttcgccctt caggtacaga accaaaaatc aagttctaca ttgcagttgt
     5101 aggggaaacc aacgaagaat cacaagctaa gattactaac atcgaagcag aaatcaatgc
     5161 atttgtaaaa taaagaccta taaaagatga gaccaaccaa cctcatcttt tttcgtatca
     5221 aatctaagca attttagaaa ctttatggca tactagactt atcaatgaaa gcgaggtaat
     5281 ctcatggaac aatttttaga taacatcaaa gaccttgaag tcactacagt tgtgcgtgcg
     5341 caagaagctc ttgataaaaa agaaactgca accttcttta tcggtcgcaa aacttgccct
     5401 tactgccgta aatttgcagg tacattgtca ggtgtcgtag ctgaaaccaa agctcacatt
     5461 tacttcatca atagtgaaga accaagccaa ctcaatgatt tgcaagcatt ccgctcacgt
     5521 tatggaatcc caactgtacc aggttttatt catattacag atggacaaat caacgtccgt
     5581 tgcgactctt caatgtcagc acaagaaatc aaagatttcg caggattgta aaaaatcagc
     5641 taacaagtct taaaatcaca aaaacgcata atgtaaggtg caaaaacctt gatattatgc
     5701 gttttattgt tcctaaaaca gttacatcag catttcatta cagaacattt taattttgga
     5761 agtcaaatca atttttaaca atgttttaga aatcacagtg ggctattcca tattcaatta
     5821 tccagattca atcgctatta tcgttttcct agattacaaa aaaaactacg ttttgctaat
     5881 caaaacgtag tctcagccac tattttatct tactgtcctt cttttacttc tttggttgct
     5941 acatcttctc caaaccattt ttggctgatt tcttggaact tgccgtcctt gtaaagacta
     6001 gaaaaagctt catttatctt cttaaccaag gttgtatctt ccttacggga tccaaccgca
     6061 aaagcttctg tttctagtcc aactgtaaag acattataat cgtttaaaac accttctgct
     6121 tctaaataat agtttgcata gacacggtca atcaatagac catcaattcg atcgtttttc
     6181 aaatcaatca aggcttcatt aaaggtttgg tattgattcg cttccttatt agcgacaata
     6241 ttcttcaaaa tttctggatt tgcttcaaag tccgcataac cagatgaacc agcttgagct
     6301 cctaatgtct ttccagtcat atcctttgca gtcgtgatac cagatgattt cttcgtaacc
     6361 aatacctgct cattcttcat atatgagtta ctgaaagcca ccttttcacg gcgttcgtct
     6421 gtagcggaat agccattcca aatcagatca atcgttcctt ttgtcaattc agcttctttc
     6481 aaatcccaat caatcggttg ccaatttacc gtgattccgt atttttcaaa aacagctgta
     6541 gctaaatcaa tatcaaatcc tgcataagaa ccatctttct gagcaaatcc cattggaaca
     6601 aaagtactat caaatccaat agtaatagac ttgttagact ggtactttga ccaattatct
     6661 ccactagttt cgctagaatt tttcccacaa gctactaaga acaaagcagt catcagactg
     6721 actaatacaa gcatccattt tttcatccat ttttcctcct atttaggttc tactttcaat
     6781 aatacatctg cgatattttc agcaaactgc aaatcatggg taaccacaat ctgggtcatc
     6841 ccaagttccc tattttgcaa gattagcttc tccacttcca aacgtaattc tggatccagg
     6901 gcagaagttg gttcatcgta gccaatgatt tctgggtcaa tcatcatagc acgcgccaaa
     6961 gccacccgct gcttttgccc accagatagt gagaaaggat aggcctctgc gtgtcctcct
     7021 agtcctaact gttccaagag cccactcgcc ttcttctcag cctcttcctg cttcattccc
     7081 atggtcttca caggcgataa agtcaaattt tccagaactg atagatgagg aaatagttga
     7141 aaatcttgga agacaaatcc cagtaggttg cgcttctgca attcatccag ctctaaaggt
     7201 tgtccattat aaaagatttg ccctgaatca atggtttcaa gacctgcaag catacgtaaa
     7261 agagttgtct tacctccacc agaaggtcca acgatagcca ggatttgctt ttcaggaata
     7321 cttagactga aatcagacag gatttgtttg tctccaaaga ctttattgat atttcgtaat
     7381 tctaacatag ctgcccccta tctataataa ctatacttct tctcaacttt tttggaaata
     7441 attgtcacaa tcccaattaa aatcaagtaa atggctcctg ccaagaacat aggaaccaga
     7501 ctagcatcgc ggttagcagc tgttcgacta gccaagataa ggtctgaaat tccgagagca
     7561 tagaccaaag aagtatcctt gaccaaactc ataacttcat taaagacact aggaagaacg
     7621 atcttggtca cttggggcaa gataatatag cgcactctgt caaaagggct aaacttcaag
     7681 accttggcag cctcatactg tcctcttgga atagtgtcaa ttcccccacg gaaaatttct
     7741 gcaaagtaag ctgcatagtt gagaacaaag gcaataatag ctgcaggaag gcggtctaaa
     7801 cgaatcccaa tacttgggag cacataatag ataaaaatca gttgcaagag taagggggtt
     7861 cctcgcataa cccagatgta aatgttaatc agataatgga ggggcttcca atggacttgc
     7921 aaggcaaagg caatcaaaac gcccaacgga atcgaaaata gcaagaccag tgcaaagacc
     7981 tgtacagtcg tgctcgcacc attcagtaaa ctcggtaata tctcaaataa ataagacata
     8041 ctgcacctcc taaaagtaat tgttcctatt atagcatgaa taaacaagat taccaagtat
     8101 ttttgtaaaa agattctacc atatacaaat aaaaaccgta gctatcaaaa gagataaact
     8161 acagttctat atgtttaaac attcttacaa tctctcaaac agttcttgca tttgcacatc
     8221 atctggaacc agttttaagt aagtgtgagc atggactttt gcttcttcaa aatgtcccaa
     8281 ttcacgcaag agatagatat agtgttccag aaattctgga ttgtccttca aatctcctgt
     8341 caactcttga taatgctcat aagcagtatc caaatcgtcc atttcttgat aagaacgagc
     8401 aatcatccac ttggtcaaaa gattttctgg ctcctcactc tgcaagtcta gaatatcctc
     8461 ataacgctcc tgctccagat aaatagtggc taaacgaagc aagatttctt ctgtatcctc
     8521 agcgtcttct tttgcagtaa ggagataatt ttctgcacca ctagcatcat gcaattcata
     8581 agaaaattgt gaagcagcta gcaagaggcg agtttcaaag ggatttttct ctaatccttg
     8641 cttagcgata tgcagggctt cttgaacttg atgttcctta tgtaaagcct gactgtaccc
     8701 atactcatag ccttcaaagt caggagaaat ggtatcaagc tgcttaaagt agagggtggc
     8761 tttttgatat tcttcttgat caaaataaag actggccaac tcaaaagctg ttaagtcatc
     8821 gtattctaac tccagggctt tttctaaaaa ctcagtagcc gtttcaaatt tccctaactg
     8881 agcataggca aagccaattc gttgataggt ggaaatgccc gtttgctcat aaatcgagcg
     8941 attatctaac tgggcatagg cttgaatagc cgcttggtaa ttttccaact cactatccaa
     9001 ctctgccaaa cccaatatca agagagaatc ctctgagtag gtcaaggcct ccaataattt
     9061 ctcacgtgcc acatctgtca aaccttccag ctggtaaagg tctgccttca aaaccaaaga
     9121 cgagacatac cagtcactgt cagcttggat ttcctcaaga taggtaaagg cttcttctat
     9181 ttgaccatcc tcgctagcaa ttgcagctag attaagatga acctctggaa aatcctctac
     9241 aattttcagg taaatttcct tggcctgagg atagaaacca atcccttcaa gataagttgc
     9301 caactcatac agaagatcac ttgaatcatt ttctaaagct ttggcgaaat aatgctcagc
     9361 cttagttaaa tcttgctcct ccaaagcctg taacatctgt tgactattgt tcactcttga
     9421 cttcctcacc ctctaattca tatagaccac tgactacctt ataccattcg aaaatggctg
     9481 aaatgacaac cttagcagag gcataaactg gaataccaag taaaactccc cagataccaa
     9541 acatagatcc tgaagttaac aaaacaaaga gaacattaat agggtggatg tttaattgac
     9601 ttcccaaaat caatggagag acaaaacggc cttcaatagt ttgttctaca ataaagacaa
     9661 tcactacttt caaaagcatg actggaccag caatcaaacc caatactagg gcaggaagca
     9721 tggctagaaa gctaccaaga taagggacca gatttaaaat accagcagta acccccagcg
     9781 taaccgcata gcgtagacca ataatcttga agaagatgat aaacattact gctacaataa
     9841 tagccactgt cacttgccct cgaacatagt tggacaactg ttgattcaca tctgatagaa
     9901 cttgtccaac aggttccttc aattttcttg gaatgaattg ggtcaaatag ttacgcaagc
     9961 ctttcccatc acgcaagaga taaaagagca tgaaaggaac gataatcaag gcaacaatca
    10021 cttgagaagc cccgctaata aaggcactca cccagttgac tgcctgagat gaaaccttac
    10081 ttgccaaaac tgtagcctgg ctagaaaaat tggtcaaaac ttgctctaat tgaggtctga
    10141 aatcatctgg cagatgctgg gctaccaatc cattaacaat cctatctata tcttctaagt
    10201 aaacaggaac gtttcttgca aaggtcaaaa cctgacgttg cagatttgga atggcgactg
    10261 ccaagcccca aatgataaag agagcgatga taacaaagac aatagtgata gctataacac
    10321 gattaacctt atgcttctcc atccaatcaa caataggatt caacaaataa tataacaaac
    10381 cagacaaaat gactggcaac atcacaactg ctaaaaagtc taaaacaggt gaaaatagaa
    10441 aactaatctt acttaaaata aaaagattca atcccaataa taaggttact aaaaataccg
    10501 taattgcctt gttatctaaa aaccacttga aaaaccaaga taggctaaaa tgtttctctt
    10561 tttgctccat aaatacttcc tttctattgt tctctcatta taccattttt gaaacaaaat
    10621 aactcttttt taaagtgctt acatctaatt ggatgtcttt aatttgcttg tttttgtgat
    10681 ataatagtct tatctttaca tagaggtata gtaatgaaag aaactgttta ttttggaact
    10741 tatacacgtc gtacttctca agggatttac aaggcagact ttgatacaga aactggtcag
    10801 ctttcaaatc tagaactttt tgcagctgag ccaagtccaa cctaccttgc ctttgaccag
    10861 caccaacatt tatacactgt tggtagccaa gacgataagg ggggaattgc agcctatcaa
    10921 actgacggga ctgtgttaaa tcatgttgtt gaagaaggag ctccccactg ttatgttgct
    10981 gtcgatgaaa agcgtgattt ggtttacgca gccaactatc acaagggaca agtccttgtt
    11041 tataaacgcc aggaagatgg tagtcttcta cttagtgata tggatcaaca cagtggccaa
    11101 ggtccacatg aaaatcaagc ttccccccat gttcactata cagatttaac acctgaccac
    11161 tatctagtga cctgcgactt gggaactgac caagtcatca cctatgatct cgatcaagaa
    11221 ggaaaattat ctaagctcta tacctatcac agcaagccag gagcaggctc acgccatatc
    11281 attttccata accactataa aatcgcttat ctcatttgtg aactcaatag tactatcgaa
    11341 gttttaatct acgatggcgt tggcgaattt gtacgtatgc aggttatttc aactttacca
    11401 gaagcttacg aaggctttaa tggaaccgct gctattcatc tctctaaaga cggtaaatac
    11461 ctctacgctt ctaaccgtgg ccatgattct atcgcagtat ataccatcct tgcggacggt
    11521 agcttagagt tattagaaat cgttccaacg catggtcaga ctccacgtga ttttgatttg
    11581 acacccgacc aaaaatttct cattgttgtc catcaagact ctgacaatgc aactgtcttt
    11641 aaacgtaatt gtgacaatgg tcgtctagca gaactctcca acgacttcca tgttcccgaa
    11701 gcagtctgca tccgttttgc tccttaaaac accataaaaa atgaacttgg tattttcaag
    11761 ttcatttttt cttatagtct atttccgaca ttaatacggt taatggcacg ttgcaaagca
    11821 atcttagcac gacgttcttg gtcaatcaaa tgtttgtctt gtgcttcttc aattgcacgt
    11881 tctgcacgaa gtttggcacg ttctgcacga ctgatatcga tatcacgagc acgttctgca
    11941 gagtcagcga cgattgtaat catatcattg gcaatttcaa taacgccacc gtttactgca
    12001 atccagttca cgtgatcttt atcatcgata cgttttacct ttacttcatc aactgctaaa
    12061 accgcaatca tattttcatg tcgtggcaag atccccatct caccatccag agttcgaacc
    12121 gatacatagc tggcatggtg atcatagacg agaccatctg gtgtcacgat ctggacagtt
    12181 aactgagcca taggtcacct cttaaaatcc cattttttca gcttttgcaa tcacatcttc
    12241 gatagaacct acaccacgga aggcatcttc tggcaagtgg tcgtatttac catcaaggat
    12301 ttccttaaag ccacgtacag tttcagcaac tggaacataa gaacctggct gaccagtaaa
    12361 ttgttccgca acgttgaagt tttgtgacaa gaagaactgg atacgacggg cgcgagcaac
    12421 caaggtcttt tcttcatcag aaagctcatc cataccaagg atagcaatga tatcttgcaa
    12481 ttcatggtaa cgttgaagga cacgttttac ttcagcagca actgcatagt gctcttctcc
    12541 aacgatttca ggtgccaagg cacgtgagct tgaagcaagt gggtcaacgg ctgggtagat
    12601 acccaattgt accaatttac gttccaagtt tgttgttgaa tccaagtgag cgaaggctgt
    12661 tgctggcgct gggtcagtat agtcatccgc tggcacatag atagcctgga tagaggttac
    12721 agaacccttc ttggttgatg tgatacgttc ttgcaattga cccatttccg tagcaagtgt
    12781 tggttggtaa ccaacggctg atggcatacg acccaaaagg gcagatactt ctgaaccagc
    12841 ctgagtgaaa cggaagatat tatcgataaa gagaagcacg tcttggcctt ccacatcacg
    12901 gaagtattca gcgattgtca aaccagtaag ggcaacacgc atacgtgctc ctggtggctc
    12961 attcatctga ccaaagacca tggctgtttt ctcgataacg cctgattctt tcatttccca
    13021 gtaaaggtca ttcccctcac gagtacgttc cccaacacca gtaaatactg aaataccacc
    13081 gtgctcttgg gcaatgttgt gaatcaattc ttggattaag acggttttac caactccggc
    13141 accaccgaaa agtccaactt taccaccttt aaggtaaggg gcaagaaggt caataacctt
    13201 gatccctgtt tcaaggattt cagaagaggt agacaactca tcaaaagttg gagctttttt
    13261 atgaattggc tgacgctctg cgtcttctgt aaaaggagct tccaaatcaa tggtatctcc
    13321 caaaacgttg aagacacgtc ccaaagtttc tttacctact ggtacagaga ttggacgacc
    13381 tgtgtccaat acttccattc cacgagtcaa cccatctgtt gattccatgg agatagtacg
    13441 aaccatacca tctcctaact ccaaggctac ttcaaggacg atttttgttt ttctttcgtc
    13501 atttttgtag acgacaagtg cattgttaat ctcaggaagt ttttcccctg ctgcaaacaa
    13561 aacgtctaca acgggaccga taacctgagc aattttacct gaactcatct ccttctccta
    13621 ttctatataa gatactgtcg gttcctagct caactaggtc ctaagttcat tttcatacga
    13681 gctggactag agcctattct aaggcactag cacctgctac gatttctgta atttcttgtg
    13741 taatcgccgc ctgtctggca cggttatact gaattgtcaa atcattgatg actttcttag
    13801 cattatctgt cgctgtttgc atggctgtca tacccgcagc attctcagct gtcttggcat
    13861 cgataatggc accgtaaatc atactttctg caaactgagg caacaactgc tccagaattt
    13921 cttctcggct ggtttccaat tcaaaagtca agctgtactc ttcatccgcc tcatttggat
    13981 ccaagtcaac aatcggaagc atttgttcca cacgcatttg actggttagc gtattgacat
    14041 ggtggttgta gcaaacataa agctcatcaa agagttcatt ttggtacatt tcaacagttt
    14101 ttgaaataat cttacgaact tgatcaaagc taggttggtc tgccaagcca cgtaattcat
    14161 aaagtggttg aataccgcga gccttaaaga aatcagctcc catcccaccg atacagatca
    14221 tttcaaaacc tttaccgtct gggtggtatt cttctttcaa ctccataaca gctttcaaaa
    14281 tagaggaatt ataacctcca accaaaccgc ggtctgaagt gataacgata tagcctgtct
    14341 tcttcacaga acggctaatc aacatcggat tagttgaagc accagctcca ttaccatgaa
    14401 ggatatctgt caaaagttta cgcactttct gagcgtaaac ttggaagttg cgagcagctt
    14461 cttcagagcg acctaactta gcagccgata ccatttgcat agcattagtg atttgactcg
    14521 tattttttgt tgaggcgatt tttgttttaa tatcatttag agacactgcc atctgacacc
    14581 tctattctta ttggaagcta gattgattga gaaactctgt aatcgcagca tccaagactg
    14641 cttcttctgg caagtctttt gtatcacgaa tggtttccaa aatctctgga tgttgagcat
    14701 caaagaaggc atggaactct tcctcgaaac gaacaatatc atctactgga acagtatcca
    14761 agaaaccatg tgtcaaagca taaagaatgg ttacttgttt ctcaacaggt aatggtttgt
    14821 gaacaggttg tttcaagacc tcaacggtac gacgtccacg gttcaactta gcctgtgttg
    14881 ctgcgtccaa gtcagaacca aacttagtaa aggcttccaa ctcacggtat gaagcaaggt
    14941 cgatacgaag tgtaccagca accttcttca tggctttgat ttgtgcagaa ccacctacac
    15001 gagatacaga tgaacccgca tcgatggctg gacgaatacc tgcattgaag aggccatcgc
    15061 caaggaagat ttgtccatca gtgatagaaa tcacgttggt tgcgatatag gctgagatat
    15121 ctcctgcttg tgtctcgata aatggtaggg ctgtaattga tccaccacca agttcatcag
    15181 aaactttagc tgagcgctca agcaaacggc tgtggagata gaaaacatcc cctgggaagg
    15241 cttcacgacc tggaggacga cgaagcaaga gcgacagttc acgataagct accgcttgtt
    15301 ttgatagatc atcgtataca atcaaaacat gcttaccttg atacataaat tcttccgcca
    15361 tagcaacccc agcataagga gctaggaaga gcaatggaga tggttgtgaa gcagaggctg
    15421 tcacaacgat tgtgtagtcc aaggcaccgt actgacgaag tgtttctact tgcgtacgaa
    15481 ctgttgattc tttttgtcca atcgcgacgt agatacagat catatcttga tctttttggt
    15541 tcaagattgt atcaatcgca atggttgttt tccctgtctg acggtcaccg ataatcaact
    15601 cacgttgacc acgaccaatc ggtacaaggg cgtcaatagc tttcaaacca gtttgcaatg
    15661 gttctgaaac agacttacgt tgcataacac caggagctgg tgcttctact ggacgagttt
    15721 tatcagtgtg gatttctcca agaccgtcaa ctggacgacc aagcggatcc acaacacgac
    15781 caatcagact ttcacctaca gggacttcca tgattttccc tgtacggcgg attgtatcgc
    15841 cttcacggat atctgtaaag tcacctagga tgataatacc aacgtctgtt gactccaagt
    15901 tttgagccat accataagag ccgttttcaa aattcaacaa ctctccactc atgacatttt
    15961 caaggccatg agcacgcgcg ataccgtccc cgatataggt tacaacacct gtttcagtca
    16021 catcaaaatt gggtttgaaa ttttcaattt gttgcttaat taaagcgctg atttcttgtg
    16081 cgttaattgc caaaagaaca ccactttcta tttcaaattt tctttaacaa ctttaagttg
    16141 ttgtttaata ctcacatcaa ttgtcttgtg attggcaaaa atgacaaaac caccaatgag
    16201 actttcatcg atttgttctt ttacactcct tactttcaga gacatttttt tctcaatcaa
    16261 agggagcaaa cgagtcttct gttcatcagt tagaggatga gcagacgtaa tcgtcacttc
    16321 aaatcgattt gtttcttttt caagtcggtt caagcaatct acaagcacat cataaaaaag
    16381 atttgctctg tgattgtagg ccagaacctg gataaagttt tgtaataaag gtgacacaga
    16441 atcttggaaa aaagcaattg ttttttcctt atccgactcg tctactgcca cttgttttaa
    16501 aaaagaaggc agacctgttt tttcaacaac ttgcttgatt tgagtcaagt ctgaaaagat
    16561 acggtcttct tctccttttt caagtaccaa ttggacaaaa ggcatgctgt atttttcaat
    16621 tacctttact gttttcttgt ccattaagct tctcctagct gatcgatata ctgatcaatg
    16681 agtgctttat gggcatgact gtcaaggttt tgtgagatga ttttaccagc taagctgatg
    16741 gtcaaatctg cgacctcacc cttaacactc tgtaaagctt ctactttatt ttgagcaatt
    16801 tcttgattgg ctttttcttt taagtgtcct gcttctagtt tagcatctgc taagatatta
    16861 gccttacttt gctcagctgt ttcctttgca ttttcaatga ttgtcttagc ttctttacgg
    16921 ctaccagcca attcatcttc gcgtttttga gccaatactt ctgctttttg acgggcttct
    16981 tcagctctgt caatatctga agcaattttt tcagctcttt cttcgaaaat gcctgtaata
    17041 ttagaccatg caaatttttt aatcaagact agcaaaagaa taaaagagcc agtgattaaa
    17101 ataaaattac caattaattc acctactgtt acgtgcatca gttactcctt tctattcctc
    17161 ttcaatatta atcttattcc ctaaatacac agatgtcaaa agagtaaaaa cataagcttg
    17221 gatgcaggaa ataaagacag aaaatgcagt ccaagccaaa ttagctccaa aggctactgg
    17281 ataccaataa atagcttggt gggaaagaag aagtaacaaa ctcgtcatga cctctcctgc
    17341 aaagatattc ccaaaaatcc gcaaagccaa agataagaag tttgtaaatt cttccaagat
    17401 attcatcggt atgacaaaaa caggactcat aaaagatttt atactttttt taaatccacg
    17461 acgacgaacg ctttctatat gtgtcaacaa aatgacaaga aaagataagg ttaagtcata
    17521 ctgtaaatta gcggttggcg aactccacca gttagtccca tcgatcgttt gaagctttgt
    17581 cattaagcca aggttattgg caatcaccat gaaaaggaat aaacaaagga aaaagagtga
    17641 gtaatctttc atgtagcgcg aaccaatgtt aggttctgta aatccaataa caaagtcata
    17701 gacatactca agtacatttt gctttccttt gggtttcaag gtcatattgc ggcttgccca
    17761 ataaataaag acaaaaataa ctcccacaat caacaaagtc atggctaaca tagtcagatt
    17821 gaagataaca ggaccaatag agatgattgg attaatactt tcttccatct aaaatcctcc
    17881 cttttcttct cgttccatgt atttatttga taataaatga gaagacaaga gttacaaaga
    17941 aagttccttc aataaaggca acacctaaaa acatcaaact acgaaactca gaaagcatat
    18001 ctggttggcg tgctactgat ttaaacagtc cattcatcaa taaaccttca ccgacagata
    18061 cgcccataca ggcaatacat aagcctaaaa atgttaaatt cataacaaat tctcctttaa
    18121 aataaaattt acttacttag tgtagtccta aaatttaaat ttgtcaactt ttaaccgcta
    18181 ttggaagcgt ttaataaaaa ttatttccat tt
//
LOCUS       DAVAAL010000030        17780 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVAAL010000030 DAVAAL010000000
VERSION     DAVAAL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.031"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            84..542
                     /locus_tag="VQM60_000849"
     CDS             84..542
                     /locus_tag="VQM60_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET1080737.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            555..1163
                     /locus_tag="VQM60_000850"
     CDS             555..1163
                     /locus_tag="VQM60_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080738.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1234..1566
                     /locus_tag="VQM60_000851"
     CDS             1234..1566
                     /locus_tag="VQM60_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080739.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQSRLISLNKSEF
                     MQLNPGTY"
     gene            1577..2992
                     /gene="ply"
                     /locus_tag="VQM60_000852"
     CDS             1577..2992
                     /gene="ply"
                     /locus_tag="VQM60_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET1080740.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            3873..4589
                     /locus_tag="VQM60_000853"
     CDS             3873..4589
                     /locus_tag="VQM60_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET1080741.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            4713..5162
                     /locus_tag="VQM60_000854"
     CDS             4713..5162
                     /locus_tag="VQM60_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET1080742.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            5201..6022
                     /locus_tag="VQM60_000855"
     CDS             5201..6022
                     /locus_tag="VQM60_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080743.1"
                     /translation="MILLAILFTCFLVYLELEVPTYISKITDLLGSQETNLDELWQPA
                     SMMMGMSFLAFLSVVAVGFFASRVAASYISRLRSDIFNRVLDYSQTEIKKFSIPSLLT
                     RTTNDITQVQMLITMGLQVVTRGPIMAIWAIGKILGHSEYWLWAVLVVVIVNVLMTTV
                     LMTLAFPKQSLIQGLTDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTRLNL
                     FVNRLMAILNPIMMGISSGLSVAIYWIGAYVINDAAPIARLPLFSDMIIFMSYAM"
     gene            6029..7060
                     /locus_tag="VQM60_000856"
     CDS             6029..7060
                     /locus_tag="VQM60_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET1080744.1"
                     /translation="MGFLLMGALFIVFPRTMVSAKRINQVLDLHSSIQNPVQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSTGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSVTAHAGQRVAIVGPTGAGKTTIVNLLMKFYEIDKRSIRI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVGQKQLLTIACALLKDASLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSIIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNS
                     QFTEDEAEE"
     gene            7126..7488
                     /locus_tag="VQM60_000857"
     CDS             7126..7488
                     /locus_tag="VQM60_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HET1080745.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFLQAPYYVPERIAITGKICGVHIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            7485..7988
                     /locus_tag="VQM60_000858"
     CDS             7485..7988
                     /locus_tag="VQM60_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET1080746.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            8102..8548
                     /locus_tag="VQM60_000859"
     CDS             8102..8548
                     /locus_tag="VQM60_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1080747.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            8553..9227
                     /locus_tag="VQM60_000860"
     CDS             8553..9227
                     /locus_tag="VQM60_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080748.1"
                     /translation="MKKKAFGIVFLVLAALILVQGNFGIPSLGGHIWPLIGIAFFAYQ
                     SVGALLRRHLTSASFTALVALMIANHFYGILPIPNQTLIWAGVLIVLGVNMLTHSNRT
                     WNGKKWWYDGEKNILIDKEVAFGTGTFYKQDQELVEDQVEVGFGNAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWCVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            9479..9667
                     /locus_tag="VQM60_000861"
                     /pseudo
     CDS             9479..9667
                     /locus_tag="VQM60_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
     gene            9917..10216
                     /locus_tag="VQM60_000862"
     CDS             9917..10216
                     /locus_tag="VQM60_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET1080749.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            10213..10530
                     /locus_tag="VQM60_000863"
     CDS             10213..10530
                     /locus_tag="VQM60_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET1080750.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            10546..11172
                     /locus_tag="VQM60_000864"
     CDS             10546..11172
                     /locus_tag="VQM60_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET1080751.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            11205..11966
                     /locus_tag="VQM60_000865"
     CDS             11205..11966
                     /locus_tag="VQM60_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET1080752.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIETLQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            12044..12439
                     /locus_tag="VQM60_000866"
     CDS             12044..12439
                     /locus_tag="VQM60_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET1080753.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            12595..12879
                     /gene="groES"
                     /locus_tag="VQM60_000867"
     CDS             12595..12879
                     /gene="groES"
                     /locus_tag="VQM60_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET1080754.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            12895..14517
                     /gene="groL"
                     /locus_tag="VQM60_000868"
     CDS             12895..14517
                     /gene="groL"
                     /locus_tag="VQM60_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET1080755.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            14655..15410
                     /locus_tag="VQM60_000869"
                     /pseudo
     CDS             14655..15410
                     /locus_tag="VQM60_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            15858..16631
                     /locus_tag="VQM60_000870"
     CDS             15858..16631
                     /locus_tag="VQM60_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET1080756.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGIGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLAS
                     LFKDEVNQDLENRTFTLQNGKRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
     gene            16644..16976
                     /locus_tag="VQM60_000871"
     CDS             16644..16976
                     /locus_tag="VQM60_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1080757.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADSISKSQLSRFERGENEISL
                     STFFELLSNINVSIENFCNHLEHYKRSERDDFLVNLSPNFYSLNIKGLEVIKNKQQKL
                     FEKSGKNS"
     gene            17014..17499
                     /locus_tag="VQM60_000872"
                     /pseudo
     CDS             17014..17499
                     /locus_tag="VQM60_000872"
                     /inference="COORDINATES: protein motif:HMM:TIGR01716.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
BASE COUNT         5490 a         3048 c         3969 g         5273 t
ORIGIN      
        1 agttatagtc cagtttttac tatactttga tattcaaata tgttatacta tggttatcaa
       61 agtaaatgaa aggagttacg aatatgacgc cagaagaaat gtacctgaca gagcgattag
      121 acgtacagat agctcatttt ttaaagaaaa gcgttcaaca tcgtaggcgc tataaggtat
      181 taaaaataac agaaatcgtg gcaggtttcc tcatagctgt cttttgtgct attcctatgc
      241 caggtgatcg ctaccgtttg atttcggttg ccttatccag tctcggcttg ctgtgtgagg
      301 ggattatcaa tttgtataat gcaaaggaaa attggatttc ttaccaaaaa actgcgcaac
      361 tcctggagaa agaaaaattc ctctatcaat gccaaacgga gaaatatgca ggaaagacca
      421 aggcttttgc cctatttgtc aagacatgcg aaggtcttat ctcagaggag attaaccagt
      481 gggaaagtat ccagtcaaaa gaagtggcag ctagtgcaga tgctccagtt aaaaaagagt
      541 aggaggtaga ggaaatgtct caatccagct acctgtcgcc cttgctctgg ttaaaaaaag
      601 aagccgataa ggaaaagatg agcgcgaccc agtgccagat atttttcttt tactatcaaa
      661 tgtttgagct cttatttgct agagaaagcg acatgaaaga cttatgtctg ggaacgaaag
      721 gtttttattt ctcgcagtta gagaaaaatt tgctttctgg agtttcccga tttctaaaaa
      781 acttggaggg gaaagtaact ctcaaggcta accaagaagt atcagctcgc aaagcccttt
      841 ttctagcctt gacaactagc caatcagatt ggcaggagtt agctcctgtt tttgattttt
      901 atcagactat cgggaggctt gaaaatcctt ctctcttgag ttctcaggac agacaacatc
      961 tgatgtggat ttaccagtca gctttggaga aggattatat tgtcaaggtt attggtgaca
     1021 agcattttgt attgaagaga caagatgcta ctaaattgac agcgcgccaa actcaaactt
     1081 tggaaattct gagtcaatca gaagacttgg tcaatcctgt ctatgttaca ttaggagaaa
     1141 agggggtgct cttgcttgat taagagagga gatgttgtag ttctttattt gccttttccg
     1201 actattagta gcgatttggc tgtgaagaat catatgtata tctgtattga caacagcatg
     1261 actaaaaaca aagagttggt taaaaatcag accttcaaac cagctctctt gaccagacgt
     1321 ttggtcaaga actttatgat agaagagccg gatctagctc gtaatccttt tacaagacca
     1381 accttgattg acttagataa ggtatttatg ttggataata cggttattcc gacttcttat
     1441 ctagccagac ggcgacgcaa tgtctcagaa gaattgtacg aggaaatttt ggatcactta
     1501 gtccaatcac ggctgatttc gctgaacaag tctgagttta tgcaactcaa tccaggaact
     1561 tattaggagg tagaagatgg caaataaagc agtaaatgac tttatactag ctatgaatta
     1621 cgataaaaag aaactcttga cccatcaggg agaaagtatt gaaaatcgtt tcatcaaaga
     1681 gggtaatcag ctacccgatg agtttgttgt tatcgaaaga aagaagcgga gcttgtcgac
     1741 aaatacaagt gatatttctg taacagctac caacgacagt cgcctctatc ctggagcact
     1801 tctcgtagtg gatgagacct tgttagagaa taatcccact cttcttgcgg tcgatcgtgc
     1861 tccgatgact tatagtattg atttgcctgg tttggcaagt agcgatagct ttctccaagt
     1921 ggaagacccc agcaattcaa gtgttcgcgg agcggtaaac gatttgttgg ctaagtggca
     1981 tcaagattat ggtcaggtca ataatgtccc agctagaatg cagtatgaaa aaatcacggc
     2041 tcacagcatg gaacaactca aggtcaagtt tggttctgac tttgaaaaga cagggaattc
     2101 tcttgatatt gattttaact ctgtccattc aggcgaaaag cagattcaga ttgttaattt
     2161 taagcagatt tattatacag tcagcgtaga cgctgttaaa aatccaggag atgtgtttca
     2221 agatactgta acggtagagg atttaaaaca gagaggaatt tctgcagagc gtcctttggt
     2281 ctatatttcg agtgttgctt atgggcgcca agtctatctc aagttggaaa ccacgagtaa
     2341 gagtgatgaa gtagaggctg cttttgaagc tttgataaaa ggagtcaagg tagctcctca
     2401 gacagagtgg aagcagattt tggacaatac agaagtgaag gcggttattt tagggggcga
     2461 cccaagttcg ggtgcccgag ttgtaacagg caaggtggat atggtagagg acttgattca
     2521 agaaggcagt cgctttacag cagatcatcc aggcttgccg atttcctata caacttcttt
     2581 tttacgtgac aatgtagttg cgacctttca aaatagtaca gactatgttg agactaaggt
     2641 tacagcttac agaaacggag atttactgct ggatcatagt ggtgcctatg ttgcccaata
     2701 ttatattact tggaatgaat tatcctatga tcatcaaggt aaggaagtct tgactcctaa
     2761 ggcttgggac agaaatgggc aggatttaac ggctcacttt accactagta ttcctttaaa
     2821 agggaatgtt cgtaatctct ctgtcaaaat tagagagtgt accgggcttg cctgggaatg
     2881 gtggcgtacg gtttatgaaa aaaccgattt gccactagtg cgtaagcgga cgatttctat
     2941 ttggggaaca actctctatc ctcaggtaga agataaggta gaaaatgact aggagaggag
     3001 aatgcttgcg acaaaaagag gcgatgatct ctctgcagat atctgaaagt ttatctcttg
     3061 cctagcgatt tccattggag tatgctcgtg atgttaaaat gaatctatca aacagaatgt
     3121 gtaaaaaagt aaaaagggta gccaattctt tgatatacag gggattgatt atcctttact
     3181 tgttttagat gattggaaat tcaagaacga cacgtatcgg aaatattttg ctgtttatat
     3241 ctaataccca tttatggaaa aaatgaaatt aaatatgaac aaatcaattg ctagcaatgg
     3301 tttagaagtg ccagtgtact agtctaactt ctccatcttg ggaacacgta aattatactc
     3361 ttcgaaaatc tcttcaaacc atgtcagccc catccgcaac ctcaaaacag tgttttgagc
     3421 aacctgaggc tagctttcta gtttgctctt tgatttttat tgagtattag aggtttgctt
     3481 agaaactctt ctattgattt cttagaatac aatagcgcct ataagaactg ttgaatttca
     3541 aagtatttac ttgaactatg atatgtctgg agtagactgt caacaaaagg tgcagtatga
     3601 ttgtttttgt cgcttatatc tacaaacttc aaacagtaat ttgagctgac tttgtcaaac
     3661 ttggctgaaa ctttctattt gcagataatg gtctagtttg ttttttgaat gtggatgagt
     3721 gtctgttgat aaagatagaa aggcaagacc atcttagcag gtaaagtgct atcctagctt
     3781 gatttgctaa taaaatccat ttcctctagt gaaaatcaaa aaaacttgtg ttataataag
     3841 aaagattaaa atgtgaaaaa aggagattcc taatgggacg taaatgggcc aatatcgtag
     3901 ccaagaaaac ggctaaagat ggagctaact ctaaagtata tgcaaaattt ggtgtagaaa
     3961 tctatgtagc agctaaaaaa ggtgatccag atccagaatc aaactcagct ttgaaattcg
     4021 ttatcgaccg tgctaaacaa gcccaagtgc caaaacacat tatcgataaa gcgattgata
     4081 aagccaaagg aaacacagac gaaaccttta cagaaggacg ttacgaaggt tttgggccaa
     4141 atggctctat gctaattgtg gatactttga cttctaacgt taaccgtaca gcagccaatg
     4201 tccgtgcagc ctttggtaaa aacggcggaa acatgggcgc ttcaggttct gtttcttacc
     4261 tctttgacaa caaaggtgtt attgtatttg gaggtgaaga tgcagacgca gtctttgagc
     4321 aattgctcga agcggatgta gatgtggatg acgtagaagc acaagaaggt acaatcacag
     4381 tttacacagc gccaactgac cttcacaagg ctatcgttgc cctccgtgag tctggtattg
     4441 aagagttcca agtgactgaa ttggaaatga ttcctcagtc agaagtggaa ttgtcaggcg
     4501 aagaccttga aacctttgaa aaactttaca gcgttcttga agacgacgaa gacgtccaaa
     4561 agatttatac gaacgtagat gggttttaat ttaacctata gaacatgatc ctaagtgaga
     4621 aaatcacttg ggattttttt aatcaagaaa atagttctca tagaaattta attattctcc
     4681 agagaactat ttttatttag aagggggaga ttatggataa accgatgtta gtctttaagc
     4741 gttttggtca tcaaattcac ctgatggtgc aaaaggaagc caaacgttgc ggcattgaat
     4801 ttatgggtgg gccgcaaggt caggttgtgc attttttgga taatcgcgag aaaaaccaag
     4861 acttggtctt gattaaagat atcgagcagg aactcaatat taccaagtct gttgctagta
     4921 atctggttaa gcgtatagtg caaaatggtt tggtggaatt ggaggcgagt cctgttgata
     4981 agcgggctaa gtttgttcgt ctgacggaca aagcacgttc tcagatgcaa caggttaagg
     5041 ctttttttga acgcatagac aagcagttga tggaagacat tgatgaagat gaattactga
     5101 tttttgagaa ggttctcggt caactacagg caaaatatca agggaatagg aggagagaat
     5161 aaagaaatta gccaaaagaa ttagtagaaa agaatggggg atgattttac tagccattct
     5221 ctttacttgc tttttggtct atctagagtt ggaagtgccg acctatatct cgaaaattac
     5281 ggatttgcta ggtagtcaag aaactaattt agatgagttg tggcagccgg caagcatgat
     5341 gatgggaatg tcctttcttg ccttcttgtc cgtagttgca gttggatttt ttgcatcccg
     5401 agtggcggct tcttatatta gtaggctgag aagtgatatt tttaaccgag ttttggatta
     5461 ctcgcagaca gagattaaga aattttcaat tcctagcctc ttgacgcgta ctaccaatga
     5521 cattactcaa gttcaaatgt tgattactat gggcttgcaa gtggtaacgc gtggtccaat
     5581 tatggctatc tgggctattg ggaagatttt aggtcattca gaatactggc tctgggccgt
     5641 acttgtggta gtgattgtca acgtcctgat gacgaccgtt ttgatgacgc tagcctttcc
     5701 aaaacagtcc ttgattcagg ggttgacaga taaactgaac agtatcactc gtgagagttt
     5761 aacaggtatt cgtgtcgttc gtgcctacaa tgcagaggat tatcaaaatg aaaaatttgc
     5821 agcagtaaat gatgaattga cccgtttgaa tttgtttgtc aaccgtctta tggctatttt
     5881 gaatcctatc atgatgggga tttcaagtgg tttgagtgta gcgatttact ggattggggc
     5941 ctatgtgatt aacgacgctg ctccgatagc gcgtctgcct ctctttagtg acatgattat
     6001 tttcatgtct tatgccatgt aggttgtcat gggcttcctt ctcatgggag cactcttcat
     6061 cgtttttccc cgaactatgg tctctgctaa gcggattaat caagttttag atttgcattc
     6121 ttctatccaa aaccctgttc aagtgcagct gactgatgaa aacttcaaag gtcaggtcga
     6181 gtttaaggat gtgaccttcc gctatgcggc aaattcggag gcagttattg aacatgttag
     6241 ctttaaagca gaaactggtc aaacagtggc ctttattggg tcaacaggtt ctggtaaatc
     6301 aactctggtc aatctgattc cacgtttcta cgacgtgtca gcaggagaaa ttctggtgga
     6361 cggtgtcaat gttcaagact atgacttctc tgtgacagct catgctggtc aaagggttgc
     6421 cattgttggg ccgactgggg ctggtaagac aaccattgtc aatcttttga tgaaattcta
     6481 tgagattgat aagagaagta ttcgcattga tggtgtggat accaaggcta tgacgcgttc
     6541 agaagtgcat gatgcctttt caatggtctt gcaggatacc tggctctttg aaggaactat
     6601 tcgagacaat ctcatctata atcaaatagg gattagtgat gaacgaatga tggaagctag
     6661 taaggctgtg ggaattcacc actttattat gaccttgcca gatggctatg ataccatctt
     6721 ggatgacacc gtgaccttgt ctgtaggaca aaaacaacta ttgactattg cttgtgccct
     6781 tcttaaggat gcatcgcttt tgattttgga tgaggcgact tcttctgttg acacacggac
     6841 agaggaattg atccaaaaag ccatggaccg tttgatggaa ggacgcacat cctttgtcat
     6901 tgcccaccgc ttgtcaatca tccgaaatgc agacttgatc ttggtcatga aagatggaaa
     6961 tatcatcgag caaggcaact atgaggaact gatggcgcaa ggtggcttct acgctgactt
     7021 gtacaatagt caatttacag aagatgaagc agaagaataa atcaaaaaaa ggcttgacgg
     7081 ccttcttttt cttatttata ctagtacaca tagaaagaga gaagtatgag tcagaaatta
     7141 tacaatatga aatttgctgc tgtctactta gccttgattg caaaagttga gcgaaagggt
     7201 ggaaaggcag agtcagttca ccaagtgacc agttggttga cagggtatga agtgagtgac
     7261 gttcttgctt gtctggatag ggatgttact tacggagatt tttttctaca agcaccatat
     7321 tatgtccctg aacgaattgc aattacaggg aagatttgcg gtgtccatat tgaggaaatt
     7381 gatgatcctc tgatgcagga aatacgtcgt ttggacaagc tagtggattg gcttgccaag
     7441 gggaaaacat ctcaacaagt tctagaaaag tatgagaagc ataaatgaaa aattatcaag
     7501 aatggtatcg aaatatcagc tccagactaa ctagtcgtcc aattctttta ttcctattac
     7561 gcagtttcaa tcgtttgatg acagtcgcca tgcccctggt ctatctgatc ttgctagtca
     7621 ccacttacct gcaactagga cttggtcagc aagttggggt ttatgtgctt attcctgcat
     7681 caggttttgt ggccttgtcc ctgtttcgta agaaaatcaa tcacccgaga ccttatgaaa
     7741 cttgggatat ctgtcccctt cttgataagg atagttcggg acggtcgatg cccagtcgcc
     7801 atgtcttttc tgcaactatc atctccatgg cctgtctgca tgctagtcta cctattggct
     7861 tagtatgctt gatcttttca gctttgctgg ggttggtgag ggttttaggg ggtgttcatt
     7921 atcccaagga tgtcttggtt ggctatgctt gtggtctggt gtggggattc cttttcttcc
     7981 tattctgaca tgtaagttaa gctagtccta caaccactta ctgcttcaaa ttgaccactt
     8041 gcttttttgc ccttgtattc ctaacttagc tttgatatag tagagtcaga aaggagatgt
     8101 gatgaagtta cgaattgaga ttgacggcaa tttagaggag actgaaattg tcatcaagac
     8161 ccccactttg acagatgaaa ttgcagactt gcaacggctc ttgcaagagt caaaggctcc
     8221 gaggttgact ttttacaagg ggacaggtga atattatcta gacctgtcag aaattctctt
     8281 ctttgaaaca gaagggagca agatctacgc tcataaccag aaggaagctt atgaggttcg
     8341 cctcaagctc tatgagttgg agtctatctt gcctcgctat tttaatcgag tttccaagtc
     8401 aacgatcgca aacatccgtc agatttactc agtggacaag tccttttcag gaacgggcac
     8461 catttccttt tatcagacgc acaaggaggt tcatgtctca cggcattacc aatccctcct
     8521 aaaagaaaat ctaagaaaca tgaggtaata agatgaaaaa gaaagcattt ggtattgtgt
     8581 tccttgtact agcagcttta atattagtac aaggaaattt tggaatccct tctctaggag
     8641 ggcacatttg gcccttgatt ggtattgctt tttttgccta ccaatcagtt ggagctttgt
     8701 tgcgtcgcca cttaacttca gcctctttta cagctctagt agccttaatg attgctaacc
     8761 acttttatgg cattttaccg attccaaacc agacactaat ctgggcagga gttctaatcg
     8821 ttctcggagt aaatatgttg actcattcta acagaacttg gaatgggaaa aaatggtggt
     8881 atgatggtga aaaaaacatt cttatagata aggaagttgc ttttgggaca gggactttct
     8941 acaagcaaga ccaagaattg gtagaagacc aagtagaagt tggttttggc aatgcaaaaa
     9001 tctactatga caatgcagag atgttaggtg attttgcaac tttaaatatt gaagtagctt
     9061 ttggtaatgc aactgtctat gttccgcaac actggtgtgt cgatttgaaa gtagaaacat
     9121 cctttggtgc agccaaggcg gacgctcctg tagcgccaac aagcaaaacc ttgattattc
     9181 gtggagacgt ggcctttggg aagttggagc ttgtctacgt taaataaaaa aatcttcact
     9241 taaaccatca aaatagacgt actaagagta ggaaattgat gccttgctct gatttcagtt
     9301 ctatggttgt tagactttaa aaaatgaaat gttgccttta aaagttgtat atttttcgat
     9361 attttggctc ttacgtttga tgtatctatg tcctacagcg tggatgatgt agtgtaaaat
     9421 gctttaaaaa aatgaatgat attggacagt tttttgaacc tttaattgct caggagccat
     9481 gaaagcaagc acttgagttt atgacaaggg agaatggtat tatgtaagtt cttctggagc
     9541 catgctagcg aatgattggg tcaaagataa tggcaagtgg tattatctag cttcatcagg
     9601 taagatgctt cgcaatacct acacacctga tggttactac gttggtaact caggcgcctg
     9661 gcaataagaa taatactctt cgaaaatctc ttcaaaccac gtcagcttca tccacaacct
     9721 caaaacagtg ttttgagctg acttcgtcag ttctatctac aacctcaaag cagtgctttg
     9781 agcagcctgc ggctagcttc ctagtttggt ctttgatttt cattgagtat aagaagccct
     9841 tttccttaaa ggaagaggct ttttacttgc ttttttgctg tttcctcatt tgtcctaaag
     9901 ccttttttgt gttaaaatga atggtatgaa agctaaaaaa atgtggatgg caggtctggc
     9961 tctgcttggt attgggagcc ttgcccttgc tacgaaaaaa gttgcagatg atcataagct
    10021 catgaagact caggaagagt tgacagcgat tgtacgagac catttttccg acatggggga
    10081 aattgcgacc ctttatgttc aagtttacga aagcagtctg gagagcttgg ttggtggcgt
    10141 catttttgag gatggccgtc attatacctt tgtctatgaa aatgaagacc tagtctatga
    10201 ggaggaagtc ttatgataca gccagcaagt ttagaagaat tagcatcttt agtggaaaaa
    10261 gcgggcaaga aggtcttcct ttttgtggca gactggtgtg gcgattgtcg ttatatttat
    10321 cctgccttac cagagattga agagaccaat ccagagttca cctttattcg aatggaccga
    10381 gatcagtata tggatttggc caaactctgg gatgtttacg gaattcctag ccttgttgtt
    10441 ctagaaaagg acaaggaaat cggtcgtttt gtcaatcgcg accgtaaaag taaggagcaa
    10501 attaacgatt ttttagcagg attgaaatag gagaaaaagg aaacaatgat ttttacgtat
    10561 aacaaagaac atgtcggtga tgtccttatg gtcatcgtga aaaatagcgg agatgccaaa
    10621 ctgaatgtgg aacgcaaagg caaggtagcc cgtgttttcc tcaaagaaaa tggggaaaca
    10681 gtagcttgga acatttttga ggtttcgagc ttgtttgaaa ttgcagagcg cggtcaagtc
    10741 tttttatcag atgagcaagt cgctcgtttg aaccaagaat tacaggcgga aggttttaca
    10801 gaagaaattg ttaatgataa ggaacctaag tttgttgttg gtgaaattgt cgagatggta
    10861 gctcatccag acagtgacca cctcaacatc tgccaagttg cagtcgcaag tgacaagata
    10921 gtgcaaatcg ttgcaggagc acctaatgcg cgtgttgggt tgaaaaccat tgtggctctt
    10981 cctggtgcca tgatgccaaa agggaatttc attttcccag gcgaacttcg tggtgaaaag
    11041 agttttggga tgatgtgtag tcctcgtgaa ttgcatttgc caaatgctcc gcaaaaacgt
    11101 ggggtgcttg aattatcaga agaccaagtt gtcggaacac cgttcgaccc agcgaaacac
    11161 tggactgcct aggaacttgt cagcatctga tagaaggaaa taagatggca aaaaatgtag
    11221 tgattacggg agcaacctca ggaatcggtg aagcgattgc gcgtgcttat ctggagcagg
    11281 gtgaggatgt cgttctaaca ggacgacgga tagacagatt agaaatcctc aagtcggagt
    11341 ttgcagtaag ctttccaaat caaaccgtct ggacttttcc actagatgtg acggatatgg
    11401 tcatggtgaa gactgtttgc tctgatattc tagaaacgat agggaggatt gatatcttgg
    11461 tcaacaacgc cggactggct cttgacttgg ctccctatca agactatgag gagttggata
    11521 tgttgaccat gttggatacc aatgttaaag gtctgatggc ggttactcgc tgtttcttgc
    11581 cagcaatgat aaaagtcaat caaggtcata ttatcaatat ggggtcaacc gcaggaatct
    11641 acgcctatgc tggtgccgct gtttactcag ctaccaaggc agcggttaag accttttcgg
    11701 atggactgcg aattgatacc atcgcaacgg atatcaaggt gacgaccatt cagcctggaa
    11761 ttgtcgaaac agatttttct acagtgcgtt ttcatggtga taaagaacga gctgcgtccg
    11821 tttaccaagg aatagaaact ttgcaagctc aggatattgc agatacagta gtctatgtga
    11881 ctagtcaacc tcgtcgtgtt cagattacag atatgaccat tatggccaat caacaggcga
    11941 caggtttcat ggttcataaa aaataaaaaa ttttctcgaa aagttacaaa tttctgtaac
    12001 tttttttgat ttcctacgaa tagataagta ggaggaagaa aatatgtata ataaagttat
    12061 cttgattgga cgtttaacgt ctacaccaga attgcacaaa accaacaatg acaagtcagt
    12121 agcgcgagca actatcgctg tcaaccgtcg ttacaaagac caaaacggtg aacgtgaagc
    12181 tgattttgtc aatatggttc tatggggcag actagcagaa actttggcaa gctacgcaac
    12241 caaaggtagt ctcatttccg ttgatggaga attgcgtacc cgtcgctttg agaaaaatgg
    12301 tcagatgaat tatgtaaccg aagtccttgt gacaggattc caactcttgg aaagtcgtgc
    12361 ccaacgtgct atgcgtgaaa ataatgcagg ccaagatttg gcagatttgg tcttggaaga
    12421 ggaagaattg ccattttaag aattaaaaag tctgagttgg tctcaggctt tttatcttga
    12481 gaaagtcaga cttttttctt gattatttct gactaagtga tacaatagaa ctatgaatta
    12541 gcactcaggt ataaagagtg ctaataatat ctatctcatt atggaggaaa tcagatgttg
    12601 aaaccattag gggaccgtgt ggtcttaaaa gtagaagaaa aagaacaaac tgttggaggc
    12661 tttgttctcg caggttcagc ccaagaaaaa accaaaacag cccaagttgt ggctactgga
    12721 caaggtgttc gtaccttgaa cggtgacttg gttgctccaa gtgttaaaac tggagatcgt
    12781 gtcttagttg aagcccacgc aggtcttgat gtcaaagatg gcgatgaaaa gtacatcatc
    12841 gtaggcgaag ctaacatttt ggcaatcatt gaggaataga aggagaaagt aagtatgtca
    12901 aaagaaatta aattttcatc agatgcccgt tcagccatgg ttcgtggtgt cgatatcctt
    12961 gcagacactg ttaaagtaac cttgggacca aaaggtcgca atgtcgttct tgaaaagtca
    13021 ttcggttcac ccttgattac caatgacggt gtgaccattg ccaaagaaat cgaattggaa
    13081 gaccattttg aaaatatggg tgctaagtta gtatcagaag tagcttctaa aaccaatgat
    13141 atcgcaggtg acggaactac gactgcaaca gtcttgaccc aagctatcgt ccgtgaagga
    13201 atcaaaaacg tcacagcagg tgcaaatcca atcggtattc gtcgtgggat tgaaacagca
    13261 gttgccgcag cagttgaagc tttgaaaaac aacgccatcc ctgttgccaa taaagaagct
    13321 atcgctcaag ttgcagccgt atcttctcgt tctgaaaaag ttggtgagta catctctgaa
    13381 gcaatggaaa aagttggcaa agacggtgtc atcaccatcg aagagtcacg tggtatggaa
    13441 acagagcttg aagtcgtaga aggaatgcag tttgaccgtg gttacctttc acagtacatg
    13501 gtgacagata gcgaaaaaat ggtggctgac cttgaaaatc cgtacatttt gattacagac
    13561 aagaaaattt ccaatatcca agaaatcttg ccacttttgg aaagcattct ccaaagcaat
    13621 cgtccactct tgattattgc ggatgatgtg gatggtgagg ctcttccaac tcttgttttg
    13681 aacaagattc gtggaacctt caacgtagta gcagtcaagg cacctggttt tggtgaccgt
    13741 cgcaaagcca tgcttgaaga tatcgccatc ttaacaggcg gaacagttat cacagaagac
    13801 cttggtcttg agttgaaaga tgcgacaatt gaagctcttg gtcaagcagc gagagtgacc
    13861 gtggacaaag atagcacggt tattgtagaa ggtgcaggaa atcctgaagc gatttctcac
    13921 cgtgttgcgg ttatcaagtc tcaaatcgaa actacaactt ctgaatttga ccgtgaaaaa
    13981 ttgcaagaac gcttggccaa attgtcaggt ggtgtagcgg ttattaaggt cggagccgca
    14041 actgaaactg agttgaaaga aatgaaactc cgcattgaag atgccctcaa cgctactcgt
    14101 gcagctgttg aagaaggtat tgttgcaggt ggtggaacag ctcttgccaa tgtgattcca
    14161 gctgttgcta ccttggaatt gacaggagat gaagcaacag gacgtaatat tgttctccgt
    14221 gctttggaag aacccgttcg tcaaattgct cacaatgcag gatttgaagg atctatcgtt
    14281 atcgatcgtt tgaaaaatgc tgagcttggt ataggattta acgcagcaac tggcgagtgg
    14341 gttaacatga ttgatcaagg tatcattgat ccagttaaag tgagtcgttc agccctacaa
    14401 aatgcagcat ctgtagccag cttgattttg acaacagaag cagtcgtagc caataaacca
    14461 gaaccagtag ccccagctcc agcaatggat ccaagcatga tgggcgggat gatgtaagca
    14521 ttctatagaa aacaacttat aaaaaacaca aaaggaggga atgactaacc cttcttttta
    14581 taatattcac ttctaaattg atttgagctc tcctaactta tatgataaaa taagactaga
    14641 aaaaggagaa gaacctgatc gatgtagaag aaattctgtg caagatgccc cccaatcaga
    14701 agattaatta tgaccgtgtc atgcagaaaa tggtagaagt atggtagaaa aatgagcaac
    14761 ggccaaccat tctcgtgcat gtttgctgtg ccccttgtag tacctataca ctagaatatt
    14821 tgaccaagta tgcagatgtg accatctatt ttgccaattc taatatccat cccaaggtag
    14881 aataccataa gcgggtctat gtcaccaaga aatttgttag tgattttaat gagcggacag
    14941 gaaatacggt tcagtaccta gaagctccct acgaacccaa ttaataccga aaactagtta
    15001 ggggactaga ggaggagccc gaaggtggcg accgttgcaa ggtttgtttt gactaccgac
    15061 tggataaaac agcgcaagtg gctatggact tgggctttga ctactttggt tcagccttga
    15121 ccatcagtcc tcataagaat tctcaaacca tcaacagcat cggaatcgat gtgcaaaaaa
    15181 tttacacgac ccactatctt cccaacgatt tcaagaaaaa tcaaggctac aaacgttcag
    15241 tagagatgtg tgaggagtat gatatctatc gtcaatgtta ttgtggctgc gtctatgcag
    15301 cccaagccca gaatattgac ctggttcaag ttaagaagga cgctacagca ttcttgttgg
    15361 gtaaggatgt tgagaaagac tattctcata tcaaatttat agtagattga aactagaata
    15421 gtacaccttt acttctaaaa cattgttaga aatcgatttg actgtcctta tttcatttta
    15481 ctatactatt acgaaattag atatataaat gaaaacttgc ctcaaggtag gtttttttgc
    15541 tagtagaaaa gcgatagaca gaaattgttg gactcttaaa aaaatgaagt tttgctcaaa
    15601 agagcttctt aaattaaaac gagcaattat cagttcaaat ctgtttcaaa agaagaacgg
    15661 ataaattaat cgatgctttg tttttgagtt aggaagttaa gcatgccaaa ctgagtcgta
    15721 ggaataaatc tctttctagt aatgtgttgt aactctgcta taatggattt attccttttt
    15781 tgcatttata caacttattt aacactatct ttattcttgt gatataatgc caaattatag
    15841 agtggaggtc atttaaaatg attgataaag taattgaaca atataaaagt catgataatc
    15901 ttgatattcg agtagaattg cataagaagt attcaaaaaa taaattggga tttaataatt
    15961 ggatattttc taactaccaa atcactgatg aagtaaaggt tctagaattg ggatgtggta
    16021 taggagaatt gtggaaaagt aatagtgatt ctatagataa aatgaagcag ttgattgtta
    16081 cagatttttc taaagatatg gtaaaatcaa cgaagtcagt gattggaaat agaaacaatg
    16141 tcaattatga gataatggat attcaaaaga tttcttttga gaatgaaact tttgatattg
    16201 ttattgctaa tatgcttcta catcatgtga atgatattcc taaagctctc tctgaggtga
    16261 atagggtctt aaaaaccgga ggaatttttt actgtgctac atttggtgaa aatggagttg
    16321 taaattattt ggcaagttta tttaaagatg aggttaatca agatttagaa aatagaacgt
    16381 ttactttaca aaatggtaaa aggtacctga gtaggtattt taactctgtc gatacattac
    16441 tctatgatga tgagttacag gtaactagta tagatgacct agttaaatac atccaatcgt
    16501 ttaaaggaat ttcagaaata ggttcactag aagaggaaat aatacgtaaa agattggaaa
    16561 gtgagtttaa taatggtatg ttgatcattc ctaaagaata tggtatgttt atcgctcgaa
    16621 aagaaagtta agggaatgaa agtgtgaaaa attacggaga agcttttcga tattttagaa
    16681 aattaaacgg atattcttta gagtatgctg cagctgattc tatatcaaaa tctcaacttt
    16741 ctagatttga aaggggagaa aatgaaattt ctctttcaac attttttgaa ttattatcaa
    16801 atatcaatgt ttcgatagag aatttttgta atcacttaga acattataaa agatcggagc
    16861 gtgatgattt tttggttaat ttatctccaa atttttatag tttgaatatt aaaggattgg
    16921 aggtaataaa aaataagcaa caaaaacttt ttgagaagag tgggaaaaac tcataaaata
    16981 aatacaatta tggttcaggg attatgaaat cagctagatt gtaatcatgt agttagtaga
    17041 gatgatctaa atttagtata tgactattta ttccagaagg aacgatggga gtcctatgag
    17101 ataacgctga ttggtaattt atatcatctt tttgaaatag attatattta catggtcgga
    17161 aaagaaatac tagagcgtac acattattac gaaaaaatcg gcaagaatag aaatttagtt
    17221 gtgtcagctt gtttaaattt ttggttctgt tgccttgaaa attcacatct aatatatgca
    17281 gactactttg aaatgaaatt acaaaaatta ttaaaagatg acacaaaagt ttttgaaaaa
    17341 tctacattca aatttgtaga aggatataaa atatacctga cagaatctaa agaatctgga
    17401 attaaacaaa tggacaatgt cataaaatat tttgagttta ttgaatctaa aagtattgct
    17461 ttatattttc aaaaacgatt aaatgagctg atagattaaa tagcattttc tctgttgaga
    17521 tattgttttt aaaatattgt actaaatgat tgatgctatg tggaaataca aaaaaatgtt
    17581 tttgatacga agttgacctg tattttttat actaatcatt ttcgtatttt ttgtattaaa
    17641 cgatataagt ttgttgtaaa cttacaagga ataaagacat taaaaaataa cagtatatct
    17701 atttgtttta tatattttac gaattctgca taaatctctt tctagtaatg tgttgtaact
    17761 ctgctataat ggatttattc
//
LOCUS       DAVAAL010000031        17541 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVAAL010000031 DAVAAL010000000
VERSION     DAVAAL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17541
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.032"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(316..2286)
                     /gene="tkt"
                     /locus_tag="VQM60_000873"
     CDS             complement(316..2286)
                     /gene="tkt"
                     /locus_tag="VQM60_000873"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET1080758.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYNDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKFP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            complement(2323..3018)
                     /locus_tag="VQM60_000874"
     CDS             complement(2323..3018)
                     /locus_tag="VQM60_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET1080759.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            complement(3029..4114)
                     /locus_tag="VQM60_000875"
     CDS             complement(3029..4114)
                     /locus_tag="VQM60_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HET1080760.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            complement(4140..4451)
                     /locus_tag="VQM60_000876"
     CDS             complement(4140..4451)
                     /locus_tag="VQM60_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HET1080761.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            complement(4479..4928)
                     /locus_tag="VQM60_000877"
     CDS             complement(4479..4928)
                     /locus_tag="VQM60_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET1080762.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            complement(4906..5334)
                     /locus_tag="VQM60_000878"
     CDS             complement(4906..5334)
                     /locus_tag="VQM60_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET1080763.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKSPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            complement(5327..6790)
                     /locus_tag="VQM60_000879"
     CDS             complement(5327..6790)
                     /locus_tag="VQM60_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET1080764.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKV
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFKMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFTELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSVFLTEGDKQRLQAKIQEINYE"
     gene            complement(7319..9655)
                     /locus_tag="VQM60_000880"
     CDS             complement(7319..9655)
                     /locus_tag="VQM60_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET1080765.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTGQTLPVYGVMMLVFTVIFILTSRYQAKK"
     gene            9788..10537
                     /locus_tag="VQM60_000881"
     CDS             9788..10537
                     /locus_tag="VQM60_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET1080766.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            complement(10830..11444)
                     /locus_tag="VQM60_000882"
     CDS             complement(10830..11444)
                     /locus_tag="VQM60_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET1080767.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(12260..12529)
                     /gene="rpsO"
                     /locus_tag="VQM60_000883"
     CDS             complement(12260..12529)
                     /gene="rpsO"
                     /locus_tag="VQM60_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET1080768.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(12670..13299)
                     /locus_tag="VQM60_000884"
     CDS             complement(12670..13299)
                     /locus_tag="VQM60_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET1080769.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     FYIDETKTTRLLDTSDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKECISLLEK"
     gene            13537..13737
                     /locus_tag="VQM60_000885"
     CDS             13537..13737
                     /locus_tag="VQM60_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080770.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            14167..14307
                     /locus_tag="VQM60_000886"
     CDS             14167..14307
                     /locus_tag="VQM60_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET1080771.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            14314..14490
                     /locus_tag="VQM60_000887"
     CDS             14314..14490
                     /locus_tag="VQM60_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET1080772.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            14548..16491
                     /gene="thrS"
                     /locus_tag="VQM60_000888"
     CDS             14548..16491
                     /gene="thrS"
                     /locus_tag="VQM60_000888"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET1080773.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(16540..17514)
                     /locus_tag="VQM60_000889"
     CDS             complement(16540..17514)
                     /locus_tag="VQM60_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET1080774.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
BASE COUNT         5392 a         3885 c         3179 g         5085 t
ORIGIN      
        1 atttcaacat cctatactat gcgtttttta attttagaga ccttctctct agctttcaaa
       61 aaacttacaa tatcctaagt caatctgtgg atgctgtgtt tattgattaa agaaactatc
      121 tagatcagta gcaaagtgat tagcgtactt cgcaaccgtt tcagagtata ctctattctg
      181 tatactatct actataactt tcgtagataa attattacat tctcattata gtttttaaat
      241 aaccaatcat ctgattagtg tctatcacta catctgctag tctgacaaca gctgtatctt
      301 cattgtaatt ggtttctata gggacttaac ttgagcaacg atattctcta ccgtaaatcc
      361 ataattatca atcacagtct gagctggggc agacgcaccg aagatgtcaa taccgatgac
      421 cgcgccatcc aaaccaacat acttgtacca actttgggtc gctgccattt caatggccac
      481 acgacgacga gtcttagatg gtaaaatgtc ttccttgtag gtagcatctt gagcatcaaa
      541 tagttcggtt gagggcatag ataccacacg tactttacca ccttgtaaaa ccaattcctt
      601 agcagctttg atagctagat tgacctcaga tcctgtagca atgataatag tatcaaatcc
      661 cggggtatca tacacgacgt aggctccttt agcgacctta ccaaagtctg tcccttcttc
      721 aactaccaag ttttgacggg ttaagacaat gacagttgga gtggtggtac tggtcaaggc
      781 atgatgccaa gccgcttgag tttcacgggc atccgctgga cggataacag tcaagtttgg
      841 cattgagcgt aaacctgcca aatgttcaac tggttcatga gttggaccat cttcaccaac
      901 ggcaattgaa tcatgggtaa agacataagt tacaggcaac tcctgaatgg ctgatagccg
      961 aatagcagct ttgacgtagt cagagaaaac aaagaaggtt ccgccataaa ctcgtaaacc
     1021 accatgaaga gccattccat tgaggattgt tcccatggca aattcacgta ccccaaactg
     1081 aatattgcgg tttaatggat tatatttatc ttgtaagcca tctgccttga tgtaggtcat
     1141 gttggagtga gctaagtctg ccgatccacc taagaaggtt ggtaaaactg ctgctgctgt
     1201 attaatagca tcttgggacg aattacgagt tgcttgagag aagccattct catagacagg
     1261 gaagtctttt tcagtaatgg ttacagggaa ttttccagct acaatagcgt caatctcact
     1321 agcaacttcg ggataagcaa ccttgtaatc agacaccaaa ctagcccaag catcgtatgc
     1381 ctcctgacca cgatccgcta catttgtctt gaaatcagaa tatacttcct ctggtacttc
     1441 aaatggatcg taatcccatc ccaaaaactt acgagttgct cctgtttctt ctgctcctag
     1501 tggtgcacca tgaacagcat ttgtaccact tttattgggt gagccgtaac caattaccgt
     1561 tttcacttca atcaaactcg gcttaccaga aaacttggcc gtctcaattg ctgtagaaat
     1621 tgctgctaaa tctgttccat cttctaccag aactgtatgc caaccataag catcgtaacg
     1681 ggcgcgaaca ttttcagaga aagtatcttt ggtctcacca tccaagcaga tgtcgttgga
     1741 gtcgtagagg acgataagct tatctaaagc ttgatgacct gcataagaag ccgcctccgc
     1801 agacactcct tccatgaagt caccgtctcc agcaataaca taagtataat ggtcaaaaat
     1861 agggaaacca tctttgttgt acttagcagc taaaaaacgc tctgcttggg cgaaaccaac
     1921 ggcagtagaa attccctgac caagcggacc agatgtcgca tccacaccag acgtatgcgt
     1981 cacttcagga tgaccaggtg tcttagatcc ccattgccgg aaatttttaa tctcgtccat
     2041 ggatacatcg ttataccctg ttaaatgcaa gagagcatac agtagcattg atccatgacc
     2101 cgcagacaag ataaagcgat ctcggttaat ccagtttggc tgctcaggtg taattctaag
     2161 gtgctttgta aataggctat aagccattgg cgcagcaccc atgacgattc ccgggtgacc
     2221 agaattagat ttgttaatag catctacgcc aagaaaacga atggcgttaa ctgattgtag
     2281 tgacatatat tcctcctaac taataaattt cttctcaaac cattatttga ttggcattgt
     2341 ttttccggtc atttcttcgt aatctctaga acagatatcc caggctttgg caatatcgtc
     2401 atccaaacca aataatccac tacgacctat aacataaata tctggtcctg ccacatcaat
     2461 ttgtttgaaa gtcttacgac tcgaagaacc atccatctca atgatgtagt gataaccatt
     2521 ctgaactcta agctgacgga gttcttggat tttatacaag gtagactcca aaaagcgttg
     2581 tcctgcaaaa cctggatcta cagtcataat agttactttg tcaagtaaat caatgtaggg
     2641 aaagattgta gaaacaggtg tttcaggatt aaggacaaca ccagccttta gacctgcatc
     2701 atgaatttta tcaatcaaac gaaaagcaag accattcaga acttcagcat gaatacaaat
     2761 atactcacat tgtaaatcga gaacttgatc tacccaaaag gttgggtctg tgaccatcag
     2821 atgaactgat aaaggtgtgt cactaatttt ttgaacttct tgaatgaacc aaggagacaa
     2881 ggtaatattg ggaacaaaat ggccatccat aatatcgata tgataagatg ctactttatc
     2941 attcaaaaaa gtaatctgct ctttgaattt gtccaaatcc atggtcatca aagatggtga
     3001 aaattcaatt cttgacatcg ttaccctctt acaaaatttc aatatctgac aaatcaatct
     3061 cttcttcaac agtcataact ggttctgtat gttttattgg ttttttcaaa atcgcgtaga
     3121 caagtcctgt gactacaatg ttggctaaca aagcacaaat accagctact ggacgagtca
     3181 tggttggtaa cataagcact ccaccaaatg gcacggcaga atcagcaccc attgtcaaag
     3241 aaacagcacc accaacagca ccaccgatac ctgttgcaat gagacctgga accaagttat
     3301 tcataacaat cggaattaca ccttcaacaa tattgacaat ccccatagga acagccgatt
     3361 tcaatgtttc gatttcctct tgagtataga tatttttttt gagtaatttc gcgataaaat
     3421 aggccaatcc aaatccaact ggtgtagcag tattcaccaa ttgcaaagca gtcaatggtt
     3481 ctttcacacc ttcagcctgt aaagtcaaca caaacgcata gactgtttta ttaagtgggc
     3541 caccaaagtc aacagcactg agaactccaa taactgcccc catcaaacca tttgaagcac
     3601 ttcccaagct ttgtaataag ctcgtcaacc agttggtaaa ggctgcgata ggcgctccaa
     3661 taatataaat cataatcaaa ctacttacca aagaggctac aaaaggaata atcaaggttg
     3721 gcattaaacc tttaatccag tttggtactt tgaccttttt aataatcgct tgaaccaaga
     3781 aaccagctat ataacctccc aagataccac cgataaaccc tgaaccaaca gaattggcaa
     3841 ttagaccaac aacaaaacct ggtgcaatcc ctggtttacc agcaatcgag taagacaaac
     3901 ctgtagcaat aacaactggc aagagaccaa gggctttacc acccatagtt gctaaagcat
     3961 cccagatagt gaattttcct gctacaagag cgtcaggaac accacccccc attgctaaac
     4021 caatggcaac taagaatcct gcaccacaaa caattggaat catataggaa atggctgtca
     4081 ataagtgacc ttttaagttt aagtgtttca acatatattt tctccttaaa tattttcagt
     4141 tatttcgtaa caatctcaac agctttagca atcagtttat tgggagattt gactgccact
     4201 tctgttggaa ccttgataat ctttttaccc tcaaagcgtt ccataccaga aatcttaaca
     4261 tcaactgcta aaataactac atccgctgca tcaatctgct cttgactcaa ttcattttct
     4321 acccctattg tcccctgagt ctcaacatga atcacatgtc cagctacctt tgcggcattc
     4381 tctaattttt cctgtgcaat ataagtgtgg gcaattccca cagtacaagc tgcaacacca
     4441 acaattttca tacggatacc ctccaaaatt ttttcttatt aacaaaaagc tgcaatcaca
     4501 tcatcagatg tctgagcccg aactaatttg gcaacaactt cgtcattacc aagttttcga
     4561 gcaaagagtg ataaggtctt caaatgctcc ctagcagctt ctgtatcatc accaactgca
     4621 aagagtacaa ttactttgac ccctttccca tcaatggtct cccaaggaat ctcattgtga
     4681 tttatagcta tgactacccc cgccttctcc acagcagaac tcttgctatg gggaatagca
     4741 atataattcc caataccggt ctgtccttct gcctctctct gataaagacc ttcgataaat
     4801 tggtctctat cagacacata acccgtctca accaatagtt gagctaatgc ctcaaaaacc
     4861 tcttctttgc tctgcatctg taaatccgtc tggatcagac tcacattaag aatatctttg
     4921 atttccatat attatctccc gtaattcttc ttttgttaac tgttttaatt gatttatgaa
     4981 tgattcatct gctagtcttc tcatcaatgt tttaatacat gacttgtcct gtgatactgc
     5041 aatggccaaa ccgataataa ggtcaacaca ctggatatcc ttcgaccatt ctctgatagg
     5101 tgattttaat ctagtaatca ctaagacatg atgttgaaag tttccttcac aatgtggtag
     5161 aagaacacct ttagcaacct ctatacttcc ctgtctctca cggtaatata gaagctcttc
     5221 tattttttct gtatcttcag aaacaagaag gctgatttga tttgctaatt ctttgtaggc
     5281 ttcttgacga ttttgaacag atatatccat aaggacaagc gaaagattat tcatagttta
     5341 tctcctgaat ttttgcttga agacgttgtt tatcaccctc ggttagaaaa acactaacta
     5401 ggacaaacgg gacacttgct ggttcctgca aagctaccgt cgtcacaatg aaatctaaat
     5461 ctggatatag atttatcagc tcatctaatt gatgataagc aactacatca ataatatcca
     5521 attcagtaaa ttgcttttct aatcgtgctc gtaaaagctc tgaagttccg acacctgatg
     5581 tacacatcac tactgttttt agaggacgtg ctcgcttttc ttgaaaccgt gcaaaatata
     5641 agactagaaa accaatctcg tccagactca aggacgcaaa accaaatact agactcactt
     5701 ctttagaaat agttgttaac tctttaacca gattaggata ggttaaaaga atttgtgata
     5761 aaagactatt cttaatctgt actctattat ctaatctcct cagtaaggga ctgatatgac
     5821 tcgccaagtc aacaaagtca ggatctgtcg tttcaatctc tctattgtcc atacacatac
     5881 ggctaaaata gtaatgagtg acatctaaaa ctcgctgaga aaaaggaagc ttcccggaag
     5941 aaaatggttt ttgcaatctc gaagatacaa cgtattgata aagatagtca atctcaactg
     6001 catcaacctt catcttaaaa tattgttcaa tttcttgaat aattttttga cagacactgt
     6061 aaattttctc atcaacaata gtaggttttg aaggtgctac aacatgaata ctagtactac
     6121 gacgattcct actgataaaa atatacaggt gagagaaaat atttatatta tatggatagg
     6181 gaatgtttac ccccaattct ctctcaagtg taatcagtaa tcccaaacaa aagtgagcaa
     6241 gttctccgtc aagaggctga accttattct gtgtaatttg ctctaaatcc aactgattaa
     6301 acattggcag tagatttaga atggctgaac gaatttgagc ctcatcccca tcaataaaaa
     6361 gctttcgttg tctcattttt aaatctaacc cataaattgc tagactctct tgtaatatct
     6421 gacgatcttt tagtactact gactcgctta cgtagaattc ctctcctaaa tcataaatag
     6481 aatgtggctt aggagcagtc aacaagagac gttctagtaa tttttcacgc cttacttcag
     6541 gatcaaaagc ctctgtaaaa ttcccatcaa cgtccacgag ggaacttctt ggatttaatt
     6601 tgaagcctct gcctttttca gataaaatga atgcttctac cggacaatct ttgttgatta
     6661 ccttgacaag gcgataaacc gttttatcag agcaatttaa tttttctccc aatactttag
     6721 cagatatata atccttacta tctcgcaaaa ggagcaacaa cgtttccatt tgttcttgtt
     6781 ttctagacac gatacacctc ctttatgatt ccattatatc gctttcaaaa aataatatac
     6841 ttcgcatttt gtcctttgca atggacattt ttgtttttat caaaaatatg atatcaaata
     6901 gaacagaacc tcactatgcc aaaatcattt taccttttct tcataacatc tattttagtt
     6961 gaaatattta agttgctttt cgtatttgat aagaaataaa ccaaacgatt ttctaataaa
     7021 taacactatg ttccatttaa caatagatag catcttcatt ttcaaactac caacctatcc
     7081 gaccatttgt tctagcaata gaagagagtt aaaattactt caatctgtcg ctttctcttt
     7141 cttacatcaa acttcaaata gtctaactac agatatttga atctattacc tcaatccaag
     7201 caggcttgag aatcactctt gggttcaccg atagataata gacatgtagg ccttgcacat
     7261 cagatctacg atagatccat cgtataaaaa actgcaatcc acagattgca gtctttcttt
     7321 attttttcgc ttggtaacga ctggtcagga tgaaaatcac ggtaaagacc aacatcatga
     7381 caccataaac aggtaatgtt tgtcctgtta aggttgaaat ttcaagcagt ttttggattc
     7441 ttgggaagag agctgtggct aggaagcctc ctactgacca gacaatcaag aggacacgcc
     7501 atagggtgaa tggcatgcag gctctaaata cggataagaa accaattgac cccaagagat
     7561 aatagaggag agttgagatt tctaactcag accaaccttg gctactccca aatattttca
     7621 caaacaggac gctgaagacg accatgagag cgcttggtag ggcacgaagc atggattttc
     7681 tgaggaaatt tggctcaaca ggtttgatat ttcgctcaaa agtcagaacg aatggtggga
     7741 aaccttccac aaactggtca atcatggtaa tctggattgg aatgaagggg aaaatcaaga
     7801 tccattcaga ccgacctagt aaagcactgg cgatacagat aactgcgagc aagaatgagt
     7861 agatggtctt tatcaagaaa atcggggcga tgtgggcaat gttattgacc acgcgacgac
     7921 cctcgaagag aatctcagga acatcattaa agtctgagtt caagagaacc agattggcaa
     7981 tctgacgggt tgctggatct ccctccgcca tcacgataga acaatccgcc tcacgaaggg
     8041 ccaagatatc attaaccccg tcccctgtca tagccgttgt atgacccgct tttttcaacg
     8101 tttggatgat gagtttcttt tgatgagggg aaacacgtcc gaaaatagct gtctcctccg
     8161 ccatggccat caattcctca tcggtgattt ttgagcaatc tacatagctg tgatagtccg
     8221 caaaaccagc cttctgggca atgctggaca ccgtaactgg attgtcacca gagataatct
     8281 tgagtcccac ctcttgagaa cggagatagt ccagcgtctc tgctgctccc tctcgaatgg
     8341 ggtccaagat ttccagcaag gctagagcct gaatatcaga tggtttctgt ggtttgtgat
     8401 ggtctaattt ctcctgactg agagctaaga ccaagacacg tgaacctctc tccaaggcct
     8461 ctctggcttc tgggacctca gaatccagca acatctcagg tgcccctaag aaaactgtcc
     8521 ccaagccttc taactccata gccccccact tgcggtcgct cgagaaggga agattggaaa
     8581 tcataggata agcaacatct cccacaaaac gctggcgaat ggcttgggca gttggattct
     8641 tatcctcact atgggccatg tagctggtca agatgctggc aatagcctct tcaccatacg
     8701 tttccgtcaa cggaagaaca gcctccacct gcatctttcc ttgggtgatg gtacccgtct
     8761 tgtccagaca gagcatatcc acgcgcgcca aggtctcaac agagtacatc tcctgcacca
     8821 agaccttttt caagcccaac ttaatcactg cagtcaagag cgaagtaatg gtcaaaaggg
     8881 caattccctt aggcaacatt cccaaaagag ctgtcgacga gtttacaacg gatgacttga
     8941 gaggcaggcc ttttaaaagc aaggcttcca gcaagagagc cagaccaaag ggaatgataa
     9001 tcttcccagt aaaaccagcc aacttgtcca gcgatttcat gatacgggag ttgatgggtt
     9061 taacggtctt ggcttccagc ataagtttgg cagcatagtt gtctgcaccg acatggtgaa
     9121 cttgagataa aactgaccca ctggctagga aacttcctga caaaagtaag ccgtcaactt
     9181 ccttttgcac caaatcactt tctcccgtta acatggcttc attgacttcc gcaaagcctt
     9241 ccaaaaccaa ggcatcacta ggaatctgct ctcctgcaga caaacgaatg acatctccta
     9301 gcactaattc ttcaggatta agagcaactt cctgaccatc acggatggtt ttgacctttt
     9361 ccttggtcat gagattgagc ttgtccacca tgtgtttggc tcgtagctcg gtcacaatcc
     9421 cagaaaaagc gttaaagcag ataacagcaa agaagaccag attgctccaa gcctgcacaa
     9481 aggcaagggc caaagcaatg gcaaagttca aagcgttaaa aagggtaaag acatttcgtt
     9541 taacgatttg ccaagtgctg gtactggctg atgcggtaaa gtcattgacc aaaccctcag
     9601 cctgtctttc cttgacttct ctttgggtta atcccataat cttattttta tccataaatt
     9661 ctattatatc atttttttta acagaattct aatttcatcc caaatatgca tctagtcaag
     9721 ggaaaagttt gccagccctc ctttgataag gtataataat aacaagaaaa caatcgaagg
     9781 agatcgcatg ttttatactt atttgcgtgg attagttgta ttgctcttat ggtccatcaa
     9841 tggcaatgct cactatcata atactgataa aattcctaat caagatgaaa attatatttt
     9901 agttgcgcct caccgtacct ggtgggatcc tgtttatatg gcctttgcga ccaagccaaa
     9961 acagttcatc tttatggcaa aaaaagaact ctttaccaac cgtatctttg gttggtggat
    10021 tcgtatgtgt ggcgcctttc ccatcgaccg tgaaaatccc agcgcctcag ccatcaaata
    10081 tcctatcaac gttctcaaaa aaagtgaccg ctctctcatc atgtttccaa gtggtagccg
    10141 ccactcaaac gatgtcaagg ggggcgcagc actgattgcc aaaatggcca aggtccgtat
    10201 catgccggtt acctacaccg gtcccatgac tttgaagggc ttgattagcc gtgaacgtgt
    10261 cgatatgaac tttggaaatc caatcgatat ctcagatatc aagaaaatga atgatgaagg
    10321 cattgaaaca gtcgccaatc gtattcaaac agaattccaa cgtctggacg aagaaacgaa
    10381 acaatggcac aatgataaaa aaccaaatcc actctggtgg tttatccgca tccctgccct
    10441 catccttgct attatcctcg ctatcctaac catcatcttt agctttatcg caagcttcat
    10501 ctggaaccca gataagaaaa gagaagaact tgcatagaag aaatgaacct tggccaaaca
    10561 gctaaggttt tcatttatat atcaaattga aactagaata gtacacctct acttcttaaa
    10621 acatttttag aaatcgattt gactgtcctg atcgatttgt cctgtttttg tttcatttta
    10681 ctatatttat aagaatctcc taatcaaatc aaaagccact ctacaataga gtaactttaa
    10741 gattgtccat ttatactcaa tgaaaaagcc ctctgaaaat cagagagctg atttgagctc
    10801 gggctaaaat cctagtgaaa caaaaaaatc tacacgatca gaaaagtctc tatcgtgcca
    10861 ttttcataca tgatgtatag tccaagtaga atgaatacta agggcacaat gattcgctcg
    10921 tatttttcaa ttgtctcgaa tattaacgga atagaggata acacccgact aatctcgcaa
    10981 aagataatta tgccgattac aaacacaagc aaggccacga gggtctgtga ccaatctaac
    11041 gaagcaaaat aaggtatata gatacctaaa ttatctccgc cagacgcaat tgtcagcaat
    11101 gtaactgtcc aaaacagttg atttgccttg ctttgttcta atctttcaat aatttcttcc
    11161 tcttcttctt ccgcatcttc tccaacaatt gcaaagcgaa tccctaaata gatagggatt
    11221 aaaccaagca atccaaccat ccattcttca ggcacgaaat taacgacata agcagcaact
    11281 aaactcgccc ctacaagtaa gcctgtgcct agatattgcc ccgcataaat atgccatttc
    11341 tgtttattct gtgatagctg tgcaaataaa ataattaaaa taattaaata atcgatactg
    11401 gtggaaatat aaacaccaat agcagatatg attgtctgtc ccataaaaaa aacctcctgt
    11461 attagtcagt aataaatcag cagattttag gagagcacag acacattaat ttagctatcg
    11521 ctagtgggta atgtcgaacg taggaaagca ttttactcct cctcaaaacg tagttacaaa
    11581 gtaatttcta actggaattc agtgactact tccatgtaaa gtataacata ctatacttta
    11641 ctttgtaaag agtgggctct tctagggggc ttgcatacag ctactcggct tgaaaagccg
    11701 agtaactgcg cacctttatg gtgtaattag ctgataccat ttgaggtttt tgtagtctcc
    11761 aaaattgtta ccgataaaac ctcaataaaa aagccctctg aagtcagaga gctgatttta
    11821 gcccgggcta aaatcctaat gaaaaagatg aaactccttg tgctcatcga acactgtgtc
    11881 atttccctat tttcatacgg atttttgacg cccttagcat catgattctt gctagataaa
    11941 tcgtttatag actaggcgac gaggcgaaga tcgtacgaaa ctttcagtga aacaaaaaaa
    12001 atctccccga agggagaaaa gctagtttat tttaccttac agtgctagga accgtaaccg
    12061 aagattatac ttgagtatag cgaggtaagg tgacaacgca aaaaagctac ccaatttaga
    12121 gtagctttat tatcaagata tgctcaattg aacacggcct aagcacttgg caaaaaagat
    12181 aaaatctcct agaaactgaa gtttcttcgt caattttcct atttttgctt tgtgcttttg
    12241 acagcctttg tatcttgaat tagcgacgaa gtcctagaga gttgatcaac tcacggtaac
    12301 ggttaacgtc gtttttacgc aagtatgcaa gcaagttacg acggcgaccg atttttttca
    12361 tcaatccacg gtaagtagcg tggtcttttt tgtgttgttt gatgtgttca ttaaggtggt
    12421 tgatttccca agtaaggaca gcaacttgaa cctctactga acctgtatca ccttcgtgac
    12481 gtgcatattg tgcaatgatt tcattttttt tctcttttga gattgccatg atgtttctcc
    12541 tttttatttg gcttcatccg agtgacaggt tggcatgcct gtaaccaaga agaagttatt
    12601 tgtctttacg acaatcccta ttttaccaag atttaaattt tctgtcaaac attaatactc
    12661 tcatagttat tatttttcaa gtaaagagat acattctttt atatgatgac ctttttcaag
    12721 ttcatctacc aatccaatag cataatctgc ataactaatc tggcttattc ctttttcatt
    12781 agtagtaaaa atttctcctg ctagaatgta gtttcctgtt ttttcaccat cgggaataaa
    12841 atctacagca ggactgacaa aagtccagtt gagattattt tttgtacgaa gaagatccaa
    12901 ttcatccgct tgagcttttg ctagtggctt aaattcttca ggaaaatcag acgtatctaa
    12961 aagtcgtgtc gtttttgttt catctatata aaagcttcct gatcccccta caactagaaa
    13021 acgtgtctgt gttccagcaa gaagctgatt aaatagttcg attgatttgc tgtggagcgg
    13081 tagcgtatct ggtgtataag caccaaacgc tgaaataaca gcatcaaatc cagtaagatc
    13141 atcttttgtc aactcaaata aatctttttt aataatagac tcagcttgac ttttgttttc
    13201 agaacgaaca atagccgtta cttcatgtcc tcgtttgact gcttcttcaa caattgcctt
    13261 ccccgcttgt ccatttgctg caataactgc tagtttcatt ttttatacct ctcttgttgt
    13321 aattatttta gttacaacaa ttgtgacact cttaataatc aatgtcaata gtcttgctta
    13381 attattatca aaatatttct accaagaaaa ctaactatga ttctagtgaa aagaaaaatc
    13441 ttctttgtca acaaatttac tttcttgttt taaacatgct ataataatca tagcaagaga
    13501 tctaagttgt ctgtttttta aaacgaggtg attatcatgc gtagattcta ttcccatctc
    13561 ccctactatc tggtcatatt attcttttat tggccacttt atgagttgtt cttactagtt
    13621 gtttctgacc cccttacact caaggaactc tatataaaca atcttctctt ctttacacct
    13681 ctggtaatct tgattgtatc gttactctat agctaccgtt tccgtttctc actttgatgg
    13741 ttagttggta acggactgct cttttacttt actatcataa cctttggtga gtttatacta
    13801 atttacttgc taatctatga aacagttgct ctggtcggca tggattctgg tattagcatc
    13861 aagcatattc tacaaaaaat gaaaaacaaa aactttcaca aaatccttga aaaatctcac
    13921 aatcatgcta taataatcca tagagacaag tcacttagtc cctttctact agagagtgcg
    13981 tggttgctgg aaacgcatag gaagtctaaa ctgatactac tcttgagttt tttatgaaaa
    14041 cataaaacgg tggccacgtt agagccgatc agaggtgtcc ctctcttttg aggtacataa
    14101 atgaaggtgg aaccacgttg cgacgtcctt tcgaggatgt cgcatttttt tattaggata
    14161 ctaattatgg agttgcaaga attagtggag cgcagttggg caatccgaca agcttatcac
    14221 gaactggaag ttaagcatca tgatttcaag tggacggtag aagaagacct cttggcttta
    14281 tctaatgata ttggaaattt ccaatgactg gtgatgacaa agcaaggacg ctactatgat
    14341 gaaacaccct acacactgga acaaaaactt tcagaaaata tctggtggct attagaactt
    14401 tctcaacgtt tggatataga cattctgacg gaaatggaaa acttcctctc tgataaagaa
    14461 aagcaattga acgttaggac tcggaagtag tctgctgata aaaaatcaat gcttagaaac
    14521 tatgaaataa taaaaaagga gaacatcatg attaacatta ctttcccaga tggcgctgtt
    14581 cgtgaattcg aatctggcgt aacaactttt gaaattgccc aatctatcag caattcccta
    14641 gctaaaaaag ccttggctgg taaattcaac ggcaaactca tcgacactac tcgcgctatc
    14701 actgaagatg gaagcatcga aattgtgaca cctgatcacg aagatgccct tccaatcttg
    14761 cgtcactcag cagctcactt gttcgcccaa gcagctcgtc gtcttttccc agacattcac
    14821 ttgggagttg gtccagccat cgaagatggt ttctactacg atactgacaa cacagctggt
    14881 caaatctcta acgaagacct tcctcgtatc gaagaagaaa tgcaaaaaat cgtcaaagaa
    14941 aacttcccat ctattcgtga agaagtgact aaagacgagg cacgtgaaat cttcaaaaat
    15001 gacccttaca agttggaatt gattgaagaa cactcagaag acgaaggcgg tttgactatc
    15061 tatcgtcagg gtgaatatgt agacctctgc cgtggacctc acgttccatc aacaggtcgt
    15121 atccaaatct tccaccttct ccatgtagct ggtgcgtact ggcgtggaaa cagcgacaac
    15181 gctatgatgc aacgtatcta cggtacagct tggtttgaca agaaagactt gaaaaactac
    15241 cttcaaatgc gtgaagaagc taaggaacgt gaccaccgta aacttggtaa agagcttgac
    15301 ctctttatga tttcacaaga agtgggacaa ggtttgccat tctggttgcc aaatggtgcg
    15361 actatccgtc gtgaattgga acgctacatc gtaaacaaag agttggcttc tggctaccaa
    15421 cacgtctaca ctccaccact tgcttctgtt gagctttaca agacttctgg tcactgggat
    15481 cattaccaag aagacatgtt cccaaccatg gacatgggtg acggggaaga atttgtcctt
    15541 cgtccaatga actgtccgca ccacatccaa gtttttaaac accatgttca ctcttaccgt
    15601 gaattgccaa tccgtatcgc tgaaatcggt atgatgcacc gttacgaaaa atctggtgcc
    15661 ctcactggcc ttcaacgtgt acgtgaaatg tcactcaacg acggtcacct attcgttact
    15721 ccagaacaaa tccaagaaga attccaacgt gcccttcagt tgattatcga tgtttatgaa
    15781 gacttcaact tgactgacta ccgcttccgc ctctctcttc gtgaccctca agatactcat
    15841 aagtactttg ataacgatga gatgtgggaa aatgcccaaa ccatgcttcg tgcagctctt
    15901 gatgaaatgg gcgtggacta ctttgaagcc gaaggtgaag cagccttcta cggaccaaaa
    15961 ttggatatcc agattaaaac tgcccttgga aaagaagaaa ccctttctac tatccaactt
    16021 gatttcttgt tgccagaacg cttcgacctc aaatacatcg gagctgatgg cgaagatcac
    16081 cgtccagtca tgatccaccg tggggttatc tcaactatgg aacgcttcac agctatcttg
    16141 attgagaact acaagggagc cttcccaaca tggctggcac cacaccaagt aaccctcatc
    16201 ccagtatcta acgaaaaaca cgtggactac gcttgggaag tggccaagaa actccgtgac
    16261 cgcggtgtcc gtgcagacgt agatgagcgc aatgaaaaaa tgcagttcaa gatccgtgct
    16321 tcacaaacca gcaagattcc ttaccaatta attgttggag acaaagaaat ggaagacgaa
    16381 acagtcaacg ttcgtcgcta cggccaaaaa gaaacacaaa ctgtctcagt tgataatttt
    16441 gttcaagcta tcctagctga tatcgccaac aaatcacgcg ttgagaaata agagtctagc
    16501 ataaaagcct ccaatctgga ggctttttct catctatttt tactcaagga ctaagttcac
    16561 ttgagcaaac tgaatccgca ctgtcgttcc ttttccgacc tcagactcga tacgaatctg
    16621 gtgccccagt tcttcagaaa ttttcttaga tagataaagg ccaagtccag aggactgctg
    16681 ggtcaaacgg ccattgtatc ctgaaaagcc acgttcaaat actcggagga catcactgtt
    16741 ttttatcccg attcccgtat ctttgataca aagctcttgg tcatccatat aaatctccag
    16801 accaccttcc ttggtgtact tgagactgtt tgagatgatt tgctcaataa ccactagcag
    16861 ccacttttta tccgtcacga tttctttatc aaggtcatgt agattgacat ttaagccttt
    16921 ttgaataaag aaaagagcat atttacgaat tatttccttg accaagtcct caatttgaac
    16981 ctgctttaag agcaaatcat catggaaact ttctaaacgc aggtactgta aaactaggtt
    17041 ggtataggag tcgattttga aaatttcctg ttctagctgc tgcttcagtt ggcggtcgac
    17101 cacttctgca actaagagtt gactggctgc aatgggggtc tttatctgat ggacccacaa
    17161 ggtatagtaa tccagcaaat ccgtcagttt tctttctgct tttgacctct gctgatagag
    17221 ttccatctca cgcgcttcta atttttctgc taaagctatt tccaaaggag acttggcttc
    17281 cctctctcca tagagaagtt cctggcgata gacctgcgtt tccaccaata tgtcccaagt
    17341 gaaaaataat atggttacaa agcaacacaa gaagaaaaag tagaggaagt aaattcctag
    17401 actggcaaat aaaaactgaa agagtaagac aagaaatgcc aaagaaagca gatagataaa
    17461 aagacgacta cgggagcgca gataggctag aaaaaattgt ttccaatcaa gcatgcttca
    17521 atccgtaccc tattcctttc t
//
LOCUS       DAVAAL010000032        17314 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVAAL010000032 DAVAAL010000000
VERSION     DAVAAL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.033"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            370..1506
                     /gene="lctO"
                     /locus_tag="VQM60_000890"
     CDS             370..1506
                     /gene="lctO"
                     /locus_tag="VQM60_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET1080775.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     regulatory      1758..1846
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2062..2730
                     /locus_tag="VQM60_000891"
     CDS             2062..2730
                     /locus_tag="VQM60_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET1080776.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            2967..3749
                     /gene="thiM"
                     /locus_tag="VQM60_000892"
     CDS             2967..3749
                     /gene="thiM"
                     /locus_tag="VQM60_000892"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET1080777.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEIAEKRPNGHLPGTFKAEFINALYEITDEDVKEFKRVK"
     gene            3751..4380
                     /locus_tag="VQM60_000893"
     CDS             3751..4380
                     /locus_tag="VQM60_000893"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET1080778.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      4589..4677
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4939..5499
                     /locus_tag="VQM60_000894"
     CDS             4939..5499
                     /locus_tag="VQM60_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET1080779.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            5500..6885
                     /locus_tag="VQM60_000895"
     CDS             5500..6885
                     /locus_tag="VQM60_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080780.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLSHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            6887..7537
                     /locus_tag="VQM60_000896"
     CDS             6887..7537
                     /locus_tag="VQM60_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET1080781.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSLANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            7548..8240
                     /gene="tenA"
                     /locus_tag="VQM60_000897"
     CDS             7548..8240
                     /gene="tenA"
                     /locus_tag="VQM60_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET1080782.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKEMEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFYISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            8245..8769
                     /gene="thiW"
                     /locus_tag="VQM60_000898"
     CDS             8245..8769
                     /gene="thiW"
                     /locus_tag="VQM60_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET1080783.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFHYSALGE
                     ILGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            8769..9572
                     /locus_tag="VQM60_000899"
     CDS             8769..9572
                     /locus_tag="VQM60_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET1080784.1"
                     /translation="MQEFTNPFPIDSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEAYE"
     gene            9565..10197
                     /locus_tag="VQM60_000900"
     CDS             9565..10197
                     /locus_tag="VQM60_000900"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET1080785.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(10406..11197)
                     /gene="thiD"
                     /locus_tag="VQM60_000901"
     CDS             complement(10406..11197)
                     /gene="thiD"
                     /locus_tag="VQM60_000901"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET1080786.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(11281..11379)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11563..11958
                     /locus_tag="VQM60_000902"
     CDS             11563..11958
                     /locus_tag="VQM60_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET1080787.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKFFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            11969..12340
                     /locus_tag="VQM60_000903"
     CDS             11969..12340
                     /locus_tag="VQM60_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET1080788.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEIMPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            12350..14593
                     /locus_tag="VQM60_000904"
     CDS             12350..14593
                     /locus_tag="VQM60_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET1080789.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLVISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            14800..16575
                     /gene="spxB"
                     /locus_tag="VQM60_000905"
     CDS             14800..16575
                     /gene="spxB"
                     /locus_tag="VQM60_000905"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET1080790.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            16686..17033
                     /locus_tag="VQM60_000906"
     CDS             16686..17033
                     /locus_tag="VQM60_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET1080791.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(17150..>17314)
                     /locus_tag="VQM60_000907"
     CDS             complement(17150..>17314)
                     /locus_tag="VQM60_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET1080792.1"
                     /translation="QVWKEIRKRGFKNKAFRTLEDVMNQLQDVIQGLEKEVIKSIVNR
                     RWTRMLFESR"
BASE COUNT         5101 a         3176 c         3825 g         5212 t
ORIGIN      
        1 aaaaaattaa taagtgggat aggaagttag cgtcaactag gatagtatct tgcttaaaca
       61 gtatatatgg gactgatata agtccatagg tcctattaga ggatgttctg gtgtcttatt
      121 cacttgtttt tttatagtat tagtagatag aatcagcaaa taaaaaagta gttcaaatgc
      181 ctggcagcaa aaaatctcaa cgattcactt aatatagact aaagtttaga gtttaaggaa
      241 agtagggcta gtatattgta tgaaaaaata agatggtttt taagcaaaac tcttgaaaat
      301 gattgaaaaa agagttatta tagttgtgta gtaaaaagca aacgcttact taatcaagga
      361 gggcatttta tgtcatacaa aacaagcaat gcagaaggtc atgtagactt catcaatacc
      421 tatgatttgg agccaatggc gcaacaagtt attcctaaag cagcatttgg ctatatcgct
      481 agtggggcgg aagatacttt cactttaaga gagaatatcc gcgcctttaa ccacaaactc
      541 atcgttcctc atacactttg cgatgttgaa aatccaagta cagagattga atttgcaggt
      601 gaaaaactat cttcaccaat cattatggcg cctgttgcgg ctcataaatt ggcaaatgaa
      661 cagggggaag tggcgactgc gcgtggtgtg catgagtttg gttctcttta tacaaccagc
      721 tcttactcta ctgttgacct tccagagatt tctgaagccc ttcaagggac accccattgg
      781 ttccaatttt actttagtaa ggatgacggt atcaaccgcc acatcatgga ccgtgtgaag
      841 gctgaaggtt ataaagcgat tgtcttgacg gcagatgcta ctgtaggggg gaatcgtgaa
      901 gtggataagc gtaatggttt tgtcttccca gttggcatgc cgattgttga agaatacctg
      961 ccagaaggtg ctggtaaatc aatggacttt gtttacaaat cagctaaaca acgcttgtct
     1021 ccacgcgatg tagaatttat cgctgaatac tctggtcttc ctgtgtatgt caagggacca
     1081 caatgccgtg aggacgttga acgttcactt gctgcaggag cttctggtat ctgggtaacc
     1141 aaccacggtg gtcgtcaaat cgacggtgga ccagctgcct ttgactcgct tcaagaagtg
     1201 gcagaagcag ttgatagacg tgtgccgatt gtctttgact ctggtgttcg tcgtggtcaa
     1261 cacgtcttta aagccttggc ttcaggagca gacttggtag ctattggtcg ccctgttatt
     1321 tatggcttgg ctctcggtgg tagtgtcggt gtgcgtcaag tctttgaaca cttgaatgcg
     1381 gaattgaaga cagttatgca attgtctgga gctcagacca ttgaagatgt caaacacttt
     1441 aagctccgtc acaatccata caacccaacc ttcccagttg accctcgtga cttaaaattg
     1501 tattgataaa acagattgcc tccactgaat gtggaggttt ttcttcgtgt attggaagaa
     1561 taaatcgcta tcgaaaacta taataaaatg aaataagaat aggacaaatc aatcaggaca
     1621 gttaaatcga tttctaacaa tgttttagaa gtagaggtgt actattctag tttcaatcta
     1681 ctatataaag atatttttcg gtcaaatatc ttgctagggc tttcattttt tgctatactg
     1741 gttcagtaat taaataatag agacatttgg ggtgctttaa gctgagatga tacccattga
     1801 acctgataca gttaagactg gcgaagggaa atgtgaaacg ttttttcgta tggcgaaatg
     1861 aacgatctaa tcttgtaagt caactctaat tcttgattgt aattggagtt ttttgtttat
     1921 ataaactgga taggcttgtt tagttagctt ctctgcaaat gctctcgatg tgctttaaaa
     1981 ggatcaagtg gaattacgat tttgcttgat atttcttggt gttttattaa ttactaacaa
     2041 gtaaagagag gagaagtaaa tatggaaaca caagactatg catttcagcc aggtttgact
     2101 gttggagaat tattaaaaag cagtcagaag gattggcagg ctgcaatcaa tcatcgtttt
     2161 gttaaggaac tttttgcggg gacaattgag aataaggtct taaaggacta tctgattcaa
     2221 gattatcact tctttgatgc cttcttatcc atgctgggtg cctgcgtagc tcacgcagat
     2281 aaacttgaat ctaagcttcg ttttgccaag caactaggct ttcttgaagc agatgaagat
     2341 ggttatttcc aaaaggcttt taaagagtta aaagtagcag agaatgacta tctagaagtg
     2401 actttgcacc ctgtaacaaa agcctttcag gatctaatgt attcggcggt ggcttcatca
     2461 gactatgccc atcttttggt catgctggtc attgcagaag gtctctattt agactggggt
     2521 tctaaagatt tggctttacc tgaagtctat attcattcgg aatggatcaa tctccacaga
     2581 ggtcctttct ttgcagagtg ggttcaattt ctggttgacg aactcaatcg tgtcggtaaa
     2641 aatcgagaag atttgacaga acttcagcaa cgctggaatc aagcagtcgc tttagaatta
     2701 gctttttttg atattggtta cgatgtctag agaaggctca tgatatttac aaatttgatg
     2761 aggtaagact cttcgaaaat ctcttcgaac cacgtcagtt ctatccacaa cctcaaaaca
     2821 gtgttttgag caacctgcgg ttagcctcct agtttgctct ttgattttca ttgagtataa
     2881 atcattcaat gtaaatgaaa aaaattctca actaaagata aatcgaaatc agtagatcgt
     2941 ataaagtgaa aaggaaggat ttcaatatga caagtttaaa attattaaaa gaaaaagcac
     3001 cattggtcat ttgtataacc aatgatgtag taaaaaattt cacagcaaat ggattagtag
     3061 cactgggcgc ctcaccagcc atgagtgagt ttccagcaga tttagaggat ttgttaaagt
     3121 atgctggtgg tttattaata aacataggaa cattgacaga tgaaaattgg aaattatacc
     3181 aagctgctct gaaaattgcg gagaaatata atgtcccagc agttttagat cctgtagcct
     3241 gtggagcagg agaatataga aaaaaagtag cagatgatct aatcaacaat tataaactag
     3301 cagcgattag aggaaatgct ggcgagattg cctctttagt aggaatagat gtggcatcta
     3361 aaggagtaga tagtgcgggc gtagataata ttgacgaaat tgctctagca gcaaatgaga
     3421 agttcaatat tccaatagta gtaacaggtg aagtggatgc cattgctgtt aatggagaag
     3481 tggtaacgat tcataatggt agtgctatga tgccgaaagt cattgggaca ggatgcttat
     3541 taggagctgt agtagcaagc tttatcggac tggaaaaagg tcaagaattg aaatcattag
     3601 aaacagcaat gttggtttac aatatcgctg gagaaatagc agaaaaacgt cccaatggac
     3661 atcttcctgg aacatttaaa gctgaattta taaatgcctt atacgagatt acagatgaag
     3721 atgtaaagga attcaaaaga gtgaagtaag atgtttcata aagaattact aaaactatat
     3781 tttatttgtg gaacgactac ttgccaagga aaaaatctat atacagtcgt tgaggaagcc
     3841 ttaaaaggtg gtataacctt atttcaattt cgagaaaaag gtgagggtgc tttagaaggc
     3901 ttagaaaaac ttgagctagc tatccaaatt aaagaacttt gtaaaaaata caatgttcca
     3961 tttatcgtta atgacgatat agatttagca atggaaattg acgccgatgg cgtacatgta
     4021 ggtcaagatg atattggtgt tgatgaaatt agaaaattga tgccagataa aataattggt
     4081 ctttctataa gaaacgaaga agaatttcaa caatcaaaag ttgaatatgt agattatgta
     4141 ggtgttgggc ctgtatttga tacccagtca aaagatgatg ctggtggtgc tataggttat
     4201 gaaggtcttg aattgatgag aaaactattg ccacaaatgc ctttagttgc aattggtggg
     4261 atacagacga aacatattaa agacattata aagaccaatg ttgacggcgt ttcaatcatt
     4321 tcggcaatat cgtatgcaaa aaatatagaa aaaactgttc gggaaatgag tgaacaatag
     4381 atatctatcc ttggattcta agtaagtagg attttccttt tttaaaaagc tatgtctaga
     4441 ttcagcttga atggctaatt catcaaacag gtcatttgtt ttttgcaaaa gaaagatctg
     4501 ctgtctttgt gagtgcaagg ctgtatttcc agttcaattt cttgaactag tataaaatgt
     4561 gtgttataat aatcacataa ttaaataata aaggcatttg gggtgcttta ggctgagatg
     4621 atacccattg aacctgatac agttaagact ggcgaaggga aatgtgaaat gtttttttcg
     4681 tatgtcgaaa agaactgtct aatcttgtaa gctgaactct aattcgtatc tgtaattaga
     4741 gttttttctt ttacgaaact ggatgaagat gatatagtag attgaaataa gatgcgaaca
     4801 aatcaattag aaaattcaaa ttaatttcta gaaatatttt agcattcctg tgtactgttc
     4861 taaattcaat ctgctataca aagtatttag gtcggtcttc ttgagtgccc cacttttctt
     4921 ttaaaaaagg agttttttat gttgaaaaaa tggcagttaa aagatgttat cttgcttgct
     4981 ttcttgtcta tcttttttgg tggggttttc gttggttcag gatatgtgta taatattctc
     5041 agtctactct taacacctct tggtttgcag gcctttgcca atgaaatcct cttcggtctc
     5101 tggtgtatgg ctgcgcccat tgctgccatc tttgttccga gagtcggaag tgcaacgatt
     5161 ggagaagtgc tagctgcgct tgctgaagtc ctttatggta gccaatttgg tctaggagct
     5221 cttttgtctg gcttagttca aggtttggga agtgaatttg gttttatcgt aactaagaat
     5281 cgctatgaaa gttggctctc tctaactgct aatagtattg ggattacgct tgttagcttt
     5341 gtctatgaat acattaagtt aggttactac gccttttccc ttccgtttgt cctttcctta
     5401 cttgtggtac gttttatttc tgtttatttc ttctgtacca tcttggttcg tgccattgtc
     5461 aaactctatc atcagtttgc aactggagga aaagcataga tgggtctgga actacgagcg
     5521 attcagtccc caatcttctc tgagccgttt gattttactt ttcatgcgca agcctttacc
     5581 ttgttagttg ggagcagtgg ttcaggaaaa tccagccttt ttcaagtcat tgcccaagtc
     5641 agttctcttc cctatagtgg tcaagtcctg atagatggga gcgaggtcag tcagctttct
     5701 atcatcgcac gtgtccagaa ggttggcatt ctctttcaaa atcccaatca tcaatttacc
     5761 atggagaact tgtttgagga gctgattttc accttggaga atatcggcta tcaccttcag
     5821 gaaattgatt ctaaaatagc agaggttgtc cagcaatgtc gttgcgaggc aatcttacac
     5881 cgtcccattc atcacctatc aggtggggaa aagcaaaaag cagcgctggc tgtcctcttt
     5941 gccatgaatc ctagggtcta tctcttggat gagcccttcg cttctattga ccgcaagagc
     6001 agaatcgaga tattggagat tctaaaagag ttggctcttg atgggaagac agttattttg
     6061 tgcgaccatg atttatctga ctataaagcc tatatcgacc atatggttga gctaagagac
     6121 ggaaaactaa gggaagtgtt tcaaatccct tcctatgaga tgacacaggt tgcttcaaag
     6181 gaagttgctt ctagcccgga actattccat atgaaccgtg tgactggtga gcttggtaat
     6241 cgccccctct tttcaattgc tgatttcaca ttctatcaag ggatttcctg tatcctgggt
     6301 gacaatggtg tcgggaaatc aaccctcttt cgctctattc ttcaatttca aaagtataag
     6361 gggcgcattg catggaaggg gacagtcctg aaaaagaaaa agagtttgta ccgtgatctg
     6421 acgggtgttg ttcaggaagc tgagaagcag tttatccgag tcagtctgcg agaggagctt
     6481 caattagatg gacctgattc tgaaagaaat cagcggattt ttcaagcttt acgatatttt
     6541 gatttggagc aggcagtcga taagagtccc tatcaattaa gtggtggtca gcaaaaaatt
     6601 cttcagctcc tgaccatctt gaccagtaag gcttccgtga tcttgctaga tgaacctttt
     6661 gcaggtttgg atgatagagc ctgccattat ttttgcaagt ggattgtgga ggagaggaat
     6721 caaggaagaa gttttctgct cattagtcat cgtttagacc ctttgatttc tgtggttgat
     6781 tattggattg agatgactag tcaggggctc agtcatgtga aagaagtgac cattaccaaa
     6841 ccacttacat ctcagagtag caatacccaa ggggaggtga gatagtatgg tcaaagtagc
     6901 aacccagaca ccgattatca gtctcttctt gctgatttta tccttggaaa catctttcat
     6961 tccttcgatt gctctgaatc tttcggtagt cgcattttgt attctcttta tgctctatta
     7021 ccgtcgattt aaaatgttag cttggatgat catacttgcc attttaccat ctttggccaa
     7081 ctactgggca gttcagttac acggagatgc ttcacaggca gtcatgcttg gaacgagggc
     7141 ctttgtgaca gtttgtatcg gccttgtctt tgtttcctct atttcactaa aagagcttct
     7201 cttgtacttg gctcaaaagg ggctatcacg ctcttggtcc tatgccttga ttgtggtatt
     7261 caattccttt cctctcattc agcaagaaat caagtccctc aaagaagctt gcctattacg
     7321 tggtcaagaa ctgcattttt ggtcgccctt gatttacagt aaggttctga tgacagtctt
     7381 taggtggcgc catctttacc tgagagctct gtctgctcat ggatatgacg aacatgcaca
     7441 gttgaagaat agctatcgga ctttttatat tcctaaaaaa acaaaattaa tctacctgct
     7501 tttcttttta ttgcttcaaa ccagtctatt tttataaagg agttattatg gaatttacag
     7561 atattgcgat ggaattatcc aagaaagctt ggcaggcttc ctttcatcat ccctttattt
     7621 tacaattaca agaggggaat ttagaacctg ccattttccg ctattacctg attcaggatg
     7681 cctactatct gaaggccttc tcagaaatct atcatctttt ggctgataag acttcaaacc
     7741 aagagatgaa aagactcttg aaacaaaatg ctcagggttt agtggagggt gagttattta
     7801 ttcgccaaca atttttcaag gaaatggaaa tcagcgacca ggaaatggag caacatccaa
     7861 ttgctccaac ctgttatcat tacatttctc atatttatag gcaatttgca gaaccgaact
     7921 tagccatcgc ttttgcaagc ttgcttcctt gtccttggtt ataccatgat ataggcaaat
     7981 cacttaatct taaaccatca ccaaatcctc tctatcaaca atggattgaa acttatatta
     8041 cggatgagtt agagcaacag atcagagagg agggagcact ggtcaatcag ctctatcgag
     8101 aaagtgacga gacagacaag caaaaaatgc tagatgcctt ctacatcagt gttcatatgg
     8161 aagccaagtt ttgggaaatg gcctaccaac accagacatg gaagagcgat ttacagtctt
     8221 tagaaaaagg agaagaatag aagcatgaga aagcaccaat tacaagttca caaattaacc
     8281 attttatcta tgatgattgc ccttgatgta gtccttacac ctatctttcg aattgaggga
     8341 atggcaccga tgtccagtgt agtcaatatt ctagcgggaa tcatgatggg acctgtttat
     8401 gccttggcca tggctacagt cacagccttt atccgtatga cgactcaagg gattccgcct
     8461 ttagctctca caggagcgac ttttggagcc cttctggcag gtctctttta taagtacggt
     8521 cgaaaatttc actattctgc tctaggagag attttgggaa caggtattat tggttctatt
     8581 gtttcctatc ctgttatggt gctctttaca ggatcagctg ctaagcttag ctggtttatc
     8641 tacacgcctc gatttttcgg agcaaccttg attggtacag cgatttcctt tattgccttt
     8701 cgatttttaa tcaagcagga attctttaaa aaagtgcagg gatatttctt tagtgaaagg
     8761 atagactgat gcaggaattt acaaatccct ttcctataga ctctagttcc ctcattcact
     8821 gtatgaccaa tgagatttct tgtgagatgc tggcaaatgg gattttggct ctgggatgca
     8881 aacctgtcat ggcagatgat ccccgtgagg ttcttgattt tactaagcaa agtcaggctc
     8941 tcttcatcaa tttggggcat ttgtcagctg agaaggagaa agcaatccgt atagcagctt
     9001 cttatgcagc tcaagtttgt ctcccaatgg tagtagatgc ggttggcgta acgacttcat
     9061 ccattcgtaa gagcttagtt aaagaccttt tagactatag acctacggtc cttaaaggaa
     9121 acatgtcaga aattcgaagt cttgttggat taaagcacca cggcgttggg gtcgatgcga
     9181 gtgctaaaga tcaagaaacg gaggatttgc ttcaagtctt gaaagactgg tgtcagacct
     9241 atcctggtat gtcattctta gttactggcc ccaaggacct catcgtttcg gagaatcaag
     9301 ttgctgtact ggagaatggc tgtactgaat tagactggat aacagggaca ggagatttgg
     9361 ttggagcctt aacagccgtt tttctcagcc aaggaaagac tggttttgaa gcttcttgct
     9421 tagcagtctc ttatctcaat atcgctgctg agaaaatagt tgttcaagga atgggattgg
     9481 aagaatttcg ttaccaagta ctcaatcagc tttcgctcct aagaagagat gaaaattggc
     9541 tagataccat caaaggagag gcttatgaat agagaagcac ttagactata tctggtaacc
     9601 aatcgctatc aagattccgt ggaaagcttt cttgcaaaag ttgagacggc ctgccgttca
     9661 ggggttacca tagtccaatt gcgagaaaaa aatctcacaa ccaatcaata ttatcaactg
     9721 gcaaaacaag tcaaggaaat aacagatgct tatcaggtac ccttgataat cgatgatcgg
     9781 ttggatgttt gtcttgcggt tgatgctgct ggtctgcata ttggagatga cgaactccca
     9841 gtttcggttg cccgacaagt cttgggtcct gaaaaaatcc tcggtgtcac cgctaaaacg
     9901 gttaaaagag ctctggaagc ggagaaatca ggtgcagatt acttggggac aggagccatt
     9961 tttccgacaa ccaccaagga aaatgcaccc atcaccctga tttcaacctt gaaaacaatt
    10021 tgccaaacgg ttgccattcc aatagttgct attggcggct tgacttcgga gaatattgat
    10081 cagcttatgg gcactggcat agctggtgta gctgttgtac gtgatttgat gcaagcagaa
    10141 gatattgagg caaaaacgca agccttttta acaaagttgc atgacattct ttcctaatta
    10201 atactcaatg aaaatcaaag agtaaactag aaagctagtc gcaggttgct caaagtactg
    10261 ccttgaggtt ttagataaga ctgacgaagt cagctcaaaa cagtgttttg aagttgcaga
    10321 tggaagctgg cgtagtttga agagattttc gaagagtata aaaactctct aattccctta
    10381 aagtgggaac tagagagttt ttttcttaat ctttaaaagt tgtatggttg actggaccag
    10441 aaccatgacc gagttgaggg gcatcttgaa tagcttttgt gataaaggcc ttggccttat
    10501 caactgcctg gtaaagactc ttgcccttgg ctagttcagc agtaatcact gcagcaaagg
    10561 tacatccagt accatgggtg tgacaggttt gaattcgtgg gctttcccag acaaattgtt
    10621 cattcttggt aaagaggaaa tctttagcac cacctttgag atgtccgcct ttgataacca
    10681 cagactgagg accaaattct tttaaaatca ggcgaccagc acgctgcatg tcttcggggt
    10741 catggattga aaaaccaaca atctcttctg cttcaggaag atttggcgta ataatagttg
    10801 ctagaggtag taagtttgtt ttgagatagt ctctagcatt tgagtcaatc aaggcatctc
    10861 cacttgtagc aaccataaca ggatcaagga catagggaca atccagtttt ttaagatagg
    10921 gttggatgat ttccatgatt tcagtagtag ccaacattcc agtttttaca gcctgaggtg
    10981 gaatatcaga aaagacactc tccaattggg ctttcaacat ttgaggagaa acgtgctcga
    11041 ttagctgaac acctctggta ttttgagcga caagactggt tacaacagcc attccataga
    11101 catctctcgc ttggaatgac tttaaatctg ccataatgcc agcaccacca ctagggtcag
    11161 tccctgcaat ggtcaaagca acgggtaaat aagtcatcta aaacctccca accaactgaa
    11221 gtttgtattc ttaagaacaa gctagtctgt tttagaaagg caatagaaga ctgctagtcc
    11281 ccattatcat ttccacttcc atcagctagc attacctaga ttgagtcaga gggatctaac
    11341 agtcgttaat ctcagcttta cgcaccccta gtggttgttt tcttttttct ttagtatagc
    11401 atatttttat tgtttatata gtaatatttg actgcaaatc gtttttcaaa tgattaaaat
    11461 tctatttttc gaagaaaata ttatttctat aattgacaaa tgtagatttt gggagtatac
    11521 tgttgagtgt aattgacaaa tgtagatttt ggaggtatgg taatgcagat ttcagatgca
    11581 gaatggcagg tcatgaagat tatttggatg cagggggagc agaccagtac agatttaatt
    11641 agggttttgg cagagcggtt tgactggtcc aagtccacgg ttcagacact tttggctcgt
    11701 ctagttgaga aagagtgttt gactcggaaa aaagaaggca agttctttgt ttattcagcg
    11761 cttttaactc tagaccaaag tcgggattta cttgtccaag atatcaaaga caaggtttgt
    11821 tcccgtagga ttaggaattt gttggctgac ttgattgtag aatgtgaatt tactcagact
    11881 gacttggaag acttggaagc tgtgatttca gagaagaaat caagtgctgt aacagaagta
    11941 agatgtaatt gtatgtaaag gagacgccat gttaaatagt attgtaacca ttatttgtat
    12001 tgcccttatc gcgtttatct tgttttggtt tttcaaaaag cctgaaaaat ctggacaaaa
    12061 agcccagcaa aaaaacggat accaagagat tcgagtggaa gtcatgggag gctatactcc
    12121 tgagttgatt gtcctcaaga aatcagtgcc agcccgcatt gtctttgacc gcaaggatcc
    12181 ttcaccatgt ctggatcaaa ttgtttttcc agattttggt gtacatgcga acctgccaat
    12241 gggggaagag tatgtagtgg aaatcatgcc tgaacaggct ggagagtttg gctttgcttg
    12301 tggtatgaac atgatgcacg gcaagatgat tgtagagtag gtggagacta tgacagaaat
    12361 tgtgaaagca agcttagaaa atggcattca aaaaatccgt atccgagctg aaaaaggcta
    12421 tcatccagcc catatccagc ttcaaaaggg aattccagct gagattacct ttcatcgtgc
    12481 tactccttca aactgttata aggaaattct gtttgaagaa gaaggtatct tggaaccaat
    12541 cggcgtagat gaggagaaag tcattcgttt tacgcctcaa gaattagggc gacatgaatt
    12601 ttcttgtggc atgaagatgc aaaagggaag ctatacagtc gttgagaaga ctcgaaaatc
    12661 tctatctctc ctgcaacgtt tttggattac tagtatcttt actgtgcctc ttgtgattct
    12721 catgattggg atgttgacag gtagcattag tcatcaagtc atgcattggg gaaccttttt
    12781 agcaacaacg cctattatgt tagttgcagg taagccatat atccagagtg cttgggccag
    12841 ttttaaaaag cacaatgcca acatggatac cttggttgcg ctgggaactc tagtggctta
    12901 tttctatagc ctagttgctc tctttgctgg tctccctgtt tacttcgaaa gtgctggatt
    12961 tatcctcttt ttcgttcttt tgggagcagt ttttgaggaa aaaatgagaa aaaatacgtc
    13021 ccaagctgtg gagaaattac tggacttgca agctaaaacc gcagaagtct tgagtgatga
    13081 tagttatgtc caagttcctt tggaacaagt caaggtaggc gacctgattc gagtgcgtcc
    13141 cggtgaaaag attgctgttg atggtgtcgt agtagaaggt gtctctagta ttgacgaatc
    13201 catggtgaca ggtgagagtc tgcctgtgga caagacagtt ggagatactg tcattggctc
    13261 aaccatcaat catagtggaa cgcttgtctt tagagcagaa aaagttggct cagagactgt
    13321 tttggctcag attgtggatt ttgtgaagaa agctcagaca agtcgtgcgc cgattcagga
    13381 cttgacggat aagatttcag ggatttttgt cccagtagtt gtcattttag gaatcatgac
    13441 cttttgggtt tggttcgtct tgctcaggga tagtgtggtt gtgcttggag ctagctttgt
    13501 gtcctctctt ctctacggag tggcggtttt gattatcgcc tgtccttgtg ccttgggact
    13561 tgcaacaccg acagccctta tggtggggac aggacgtagt gccaagatgg gggttctcct
    13621 caaaaatgga actgtcttac aggaaatcca gaaagttcaa actcttgtct ttgataagac
    13681 cgggactttg acggaaggga aacctgtggt aacagatgtc atcggcgacg aagtagaagt
    13741 gtttgggttg gcagcctcct tggaagatgc ttctcaacac ccactggctg aggctatcgt
    13801 taagcgagcg agtgaagctg gacttgagtt tcaaactgtt gaaaattttc agaccttgca
    13861 cgggaaaggt gtttcagggc gaatcaatgg aaaacaagtt ttacttggaa atgctaaaat
    13921 gctggatggc atggatattt ctaatactta tcaagataaa ctagaagaac tagaaaaaga
    13981 agctaagaca gttgtgttct tagctgttga caatgaaatc aaaggcttgc ttgctttgca
    14041 agatattcct aaggaaaatg ctaagctagt catcagtcag ctaaaaaaac gtggtctccg
    14101 aacagtcatg ctgacaggag acaatgctgg tgtggcgcgt gctattgcag atcaaatcgg
    14161 aattgaagag gtcattgcag gcgtcttgcc agaagaaaaa gcccatgaaa tccataaact
    14221 gcaacagtca gggaaagtag cctttgttgg ggacggtatc aatgacgctc ctgcccttag
    14281 tgtagcagat gtggggattg ctatgggagc tggaacagat atcgccatcg aatcagcaga
    14341 tttggtgttg acaaccaata atcttttagg agtggttcgt gcctttgata tgagtaagaa
    14401 aacctttcat cgaattctac tcaatctttt ctgggctttt atctacaatg ttgttggaat
    14461 tccgattgca gcaggagtct tttcaggtgt tggactggct ctcaacccag aattggcagg
    14521 tctagccatg gcctttagtt ctgtatctgt tctgaccagt tcccttctgt taaattttag
    14581 taaaatagac taaaagtaat gactagattt ctttgttata aaacagaaat gacaacactt
    14641 tcaaaactgt attataatag agaatagaga gttttgagca gatttttaga aaagtcagca
    14701 taaatatgat acagtggaat agtaaaaatt tggagaacgt ttccaattct atgtaatcgt
    14761 attctccaag tctaaaaaaa ttgaaggaga gttatcatta tgactcaagg gaaaattact
    14821 gcatctgcag caatgcttaa cgtattgaaa acatggggcg tagatacaat ctacggtatc
    14881 ccatcaggaa cactcagctc attgatggat gctttggctg aagacaaaga tatccgtttc
    14941 ttacaagttc gccacgaaga gacaggtgct cttgcagcgg ttatgcaagc taaattcggc
    15001 ggctcaatcg gggttgcagt tggttcaggt ggtccaggtg cgactcactt gattaacggt
    15061 gtttacgatg cagctatgga taacactcca ttcctagcga tccttggatc acgtccagtt
    15121 aacgaattga acatggatgc tttccaagag cttaaccaaa acccaatgta caacggtatc
    15181 gctgtttaca acaaacgtgt agcttacgct gagcaattgc caaaagtaat tgacgaagcc
    15241 tgccgtgctg cagtttctaa aaaaggtcca gctgttgttg aaattccagt aaacttcggt
    15301 ttccaagaaa tcgatgaaaa ctcatactac ggttcaggtt catacgaacg ctcattcatc
    15361 gctcctgctt tgaacgaagt tgaaatcgac aaagctgttg aaatcttgaa caatgctgaa
    15421 cgcccagtta tctatgctgg atttggtggt gttaaagctg gtgaagtgat tactgaattg
    15481 tcacgtaaaa tcaaagcacc aatcatcaca actggtaaaa actttgaagc tttcgaatgg
    15541 aactatgaag gtttgacagg ttctgcttac cgtgttggtt ggaaaccagc caacgaagtg
    15601 gtctttgaag cagacacagt tcttttcctt ggttcaaact tcccatttgc tgaagtttac
    15661 gaagcattca agaacactga aaaattcatc caagtcgata tcgaccctta caaacttggt
    15721 aaacgtcatg cccttgacgc ttcaatcctt ggtgatgctg gtcaagcagc taaagctatc
    15781 cttgacaaag taaacccagt tgaatcaact ccatggtggc gtgcaaacgt taagaacaac
    15841 caaaactggc gtgattacat gaacaaactc gaaggtaaaa ctgagggtga attgcaattg
    15901 tatcaagttt acaatgcaat caacaaacat gctgatcaag acgctatcta ctcaatcgac
    15961 gtaggtaaca ctactcaaac atctactcgt caccttcaca tgacacctaa gaacatgtgg
    16021 cgtacatctc cactctttgc gacaatgggt attgcccttc ctggtggtat cgctgctaag
    16081 aaagacaatc cagatcgcca agtatggaac atcatgggtg acggagcatt caacatgtgc
    16141 tacccagacg ttatcacaaa cgttcaatac gaccttccag ttatcaacct tgtcttctca
    16201 aatgctgagt acggcttcat caagaacaaa tacgaagata caaacaaaca cttgtttggt
    16261 gtagacttca caaacgctga ctacgctaaa attgcggaag ctcaaggagc tgttggattc
    16321 acagttgacc gtatcgaaga catcgatgca gttgttgcag aagctgttaa attgaacaaa
    16381 gaaggtaaaa ctgttgtcat cgatgctcgc atcactcaac accgtccact tccagtagaa
    16441 gtacttgaat tggatccaaa acttcactca gaagaagcta tcaaagcctt caaggaaaaa
    16501 tacgaagcag aagaactcgt accattccgt ctcttcttgg aagaagaagg attgcaatca
    16561 cgcgcaatta aataattcct ctcgccgaaa atcaaatatg aaacttgtag actttattct
    16621 ttgattttca tagagcagaa ctttaaaaga tcggagcaat ccggtctttt tatggaggag
    16681 agtaaatgaa tttaaatcaa ttagatatta tcgtttcaaa tgttccccaa gtctgtgctg
    16741 acttggagca tatcttggat aaaaaggcag attatgctga tgatggtttt gctcagttta
    16801 cgattggtag tcactgcctt atgttgtcac aaaatcattt ggttcctttg gaaaactttc
    16861 agtcaggaat cattattcat atcgaggttg aggatgtaga ccagaactac aaacggttga
    16921 acgagcttgg tataaaggtt ttacacggac caactgtaac cgattgggga acagagtcct
    16981 tattagttca aggacctgct ggtctagtgc ttgattttta tcgtatgaaa taggtcgcgt
    17041 agtcatttga cgttaacctt agctattttt aaaatagaaa ttgagaatat gaactattga
    17101 aagatcggag caatccggtc tttttatata aagaaattgc atataatact catctgcttt
    17161 caaaaagcat tctagtccat ctccgattaa cgatggactt tatcacctcc ttctccaatc
    17221 cttgtatgac atcttggagt tgattcatga catcttccaa agttcgaaag gctttattct
    17281 taaatccacg tttacgaatc tctttccaca cttg
//
LOCUS       DAVAAL010000033        16177 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVAAL010000033 DAVAAL010000000
VERSION     DAVAAL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16177
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.034"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..984
                     /gene="lytB"
                     /locus_tag="VQM60_000908"
     CDS             <1..984
                     /gene="lytB"
                     /locus_tag="VQM60_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HET1080793.1"
                     /translation="SDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGK
                     WLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITIS
                     GLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVAFHLSDMEVGKKYYS
                     ADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEH
                     YHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGAT
                     KWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(1045..2700)
                     /gene="pavA"
                     /locus_tag="VQM60_000909"
     CDS             complement(1045..2700)
                     /gene="pavA"
                     /locus_tag="VQM60_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET1080794.1"
                     /translation="MSFDGFFLHHIVEELRNELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            2805..3302
                     /gene="ybeY"
                     /locus_tag="VQM60_000910"
     CDS             2805..3302
                     /gene="ybeY"
                     /locus_tag="VQM60_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET1080795.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            3283..3678
                     /locus_tag="VQM60_000911"
     CDS             3283..3678
                     /locus_tag="VQM60_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET1080796.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            3695..4594
                     /gene="era"
                     /locus_tag="VQM60_000912"
     CDS             3695..4594
                     /gene="era"
                     /locus_tag="VQM60_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET1080797.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            4643..5467
                     /gene="mutM"
                     /locus_tag="VQM60_000913"
     CDS             4643..5467
                     /gene="mutM"
                     /locus_tag="VQM60_000913"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET1080798.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            5467..6072
                     /locus_tag="VQM60_000914"
     CDS             5467..6072
                     /locus_tag="VQM60_000914"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET1080799.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKSGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDTQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            6059..7258
                     /locus_tag="VQM60_000915"
     CDS             6059..7258
                     /locus_tag="VQM60_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HET1080800.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEVFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            7258..7407
                     /gene="rpmG"
                     /locus_tag="VQM60_000916"
     CDS             7258..7407
                     /gene="rpmG"
                     /locus_tag="VQM60_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET1080801.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            7447..7680
                     /gene="secG"
                     /locus_tag="VQM60_000917"
     CDS             7447..7680
                     /gene="secG"
                     /locus_tag="VQM60_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET1080802.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            7782..10136
                     /gene="rnr"
                     /locus_tag="VQM60_000918"
     CDS             7782..10136
                     /gene="rnr"
                     /locus_tag="VQM60_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET1080803.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            10099..10566
                     /gene="smpB"
                     /locus_tag="VQM60_000919"
     CDS             10099..10566
                     /gene="smpB"
                     /locus_tag="VQM60_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET1080804.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            10582..11442
                     /gene="tehB"
                     /locus_tag="VQM60_000920"
     CDS             10582..11442
                     /gene="tehB"
                     /locus_tag="VQM60_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET1080805.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTKPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDIPVDLYDINSASIGQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            11515..12468
                     /gene="coiA"
                     /locus_tag="VQM60_000921"
     CDS             11515..12468
                     /gene="coiA"
                     /locus_tag="VQM60_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET1080806.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            12487..14298
                     /gene="pepF"
                     /locus_tag="VQM60_000922"
     CDS             12487..14298
                     /gene="pepF"
                     /locus_tag="VQM60_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET1080807.1"
                     /translation="MVLQRNERNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVA
                     QYEGHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLY
                     SQLDQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELL
                     AGAGEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAY
                     QALYATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAA
                     VRKHLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALKKAEDALAVLGE
                     DYLSRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETG
                     HSMHSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLD
                     GFRGTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQY
                     EWARIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVM
                     RKAGVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            14300..15013
                     /locus_tag="VQM60_000923"
     CDS             14300..15013
                     /locus_tag="VQM60_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET1080808.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFYATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            15080..16021
                     /gene="prsA"
                     /locus_tag="VQM60_000924"
     CDS             15080..16021
                     /gene="prsA"
                     /locus_tag="VQM60_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET1080809.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
BASE COUNT         4883 a         3029 c         3638 g         4627 t
ORIGIN      
        1 tctgatggca aaatggctga aaaagaatgg gtctacgatt ctcatagtca agcctggtac
       61 tacttcaaat ctggtggcta catggcgaaa aatgagacag tagatggtta tcagcttgga
      121 agcgatggta aatggcttgg aggaaaagct acaaatgaaa atgctgctta ctatcaagta
      181 gtgcctgtta cagccaatgt ttatgattca gatggtgaaa agctttccta tatatcgcaa
      241 ggtagtgtcg tatggctaga taaggataga aaaagtgatg acaagcgctt ggctattact
      301 atttctggtt tgtcaggcta tatgaaaaca gaagatttac aagcgctaga tgctagtaag
      361 gactttatcc cttattatga gagtgatggc caccgttttt atcactatgt ggctcagaat
      421 gctagtatcc cagtagcttt tcatctttct gatatggaag taggcaagaa atattattcg
      481 gcagatggcc tgcattttga tggttttaag cttgagaatc ccttcctttt caaagattta
      541 acagaggcta caaactacag tgctgaagaa ttggataagg tatttagttt gctaaacatt
      601 aacaatagcc ttttggagaa caagggcgct acttttaagg aagccgaaga acattaccat
      661 atcaatgctc tttatctcct tgcccatagt gccctagaaa gtaactgggg aagaagtaaa
      721 attgccaaag ataagaataa tttctttggc attacagcct atgatacgac cccttacctt
      781 tctgctaaga catttgatga tgtggataag ggaattttag gtgcaaccaa gtggattaag
      841 gaaaattata tcgatagggg aagaactttt cttggaaaca aggcttctgg tatgaatgtg
      901 gaatatgctt cagaccctta ttggggcgaa aaaattgcta gtgtgatgat gaaaatcaat
      961 gagaagctag gtggcaagga ttagtactat aagtgaatat gatttgagtg aatagtaagt
     1021 taaaaatcct gatttcaagt aaaatcagga ttttttcatg gatgcaattt ttttggagtc
     1081 tggtgtgacg cggagggtct tttgtcctgt gtaagtgaca aagccgggtt ttccaccagt
     1141 tggtttattg agttttttga cttcaatcat atctacctgc accagattcg acaggcgccc
     1201 ttgagagaag taggcagcta actctgctgc gtctgtcttg actgcatcag atgggtcaag
     1261 atttcctgag atgacaacat ggcttccagg aatgtcctta gcatggaacc aaagttcctc
     1321 cttgcgggcc attttaaagg tcaattcctc attttgaaga ttgtttcgtc cgacatagat
     1381 gatggttttg ccatcgcttg ctagatattg ttctagtttt ttgcgtttct ggattttctc
     1441 ccgttgtctt ctgcggataa aacctgtttg aatcaattct tcacggattt cagcgatttc
     1501 ttccagtcca gcttggttga ggacggtttc tacactttcc agatagagaa tagtggcttt
     1561 ggtttcttca atcaaatcag tcaagtattt gacagcttct ttgagtttct gataccgttt
     1621 aaaatagcgt tgggcattct ggttgggagt cagagcctta tcaagcgcaa tcatgatagg
     1681 ttggttggta tagtagttgt ctaggataac ctggtcttgg tcgttaggca cttggtgtag
     1741 gaaggttgtc agcaattctc ctttttgacg aaattcttca gcgttgtctg tcgccagtaa
     1801 ctctttttcc tgttttttga gtttgtgtcg gtttttctga agttcatttt caacacgacg
     1861 aatcagttca ctggcctgct gtttgacgcg gtcgcgctca gccttatcct tatagtaggt
     1921 gtccaacaaa tcagaaagat ttgcaaaagg ctctcccacc tgatttgcaa aaggaactgg
     1981 actgaaggaa gtctcagtca agcatggctt ggtttcttga ttgaaaaaat ttcggaaagc
     2041 ggaaagtttt tcactaacca gtatcctttc caattcattt gccgtatcgc gtcccagacc
     2101 ttgaaagagg ctttgaagat tttttgctgt tagttcttgg gtttgcagga tttcaaagag
     2161 cttttcatcc ttgatagtaa aaggattgag agattctgta cttggcggag cgatataggt
     2221 cgatcctgga agtaaggtgc ggtagctatt ttgtgaaaaa ccgacgtgtt tgataacttc
     2281 gaggatttta tgactgcttt tatcgaccag tagaatatta ctgtgtttcc ccataatttc
     2341 aataatcaag gtagcctgga tatggtctcc aatctcgttt ttattggaaa ctgtcatttc
     2401 cacaatacgg tcattttcca cttgctcaat cgactcaatc agggccccct gcaaatactt
     2461 tctcaaaacc atgataaagg tagaaggttg agctggattt tcaaaagtcg tttgggtcag
     2521 ctgaatgcgt ccaaaaactg gatgggcaga aaggagcagg cgatggcttt ggcgattgct
     2581 gcggatttgc aagaccaact cttgttcaaa aggctgattg attttctgga tgcgaccatt
     2641 cactaattcg tttcgcaatt cctcaactat gtggtgtaaa aaaaatccat caaatgacat
     2701 cgttctctcc ttgtgattgt attccatagt attatatcaa aaaggtagaa taaaatcatg
     2761 gaaatgtggt ataataaagc caagtaaaga gaaatgagaa gcacatgtat attgaaatgg
     2821 tagatgaaac tggtcaagtt tcaaaagaaa tgttgcaaca aacccaagaa attttggaat
     2881 ttgcagccca aaaattagga aaagaagaca aggagatggc agtcactttt gtgaccaatg
     2941 agcgtagtca tgaacttaat ctggagtacc gtgacaccga ccgtccgaca gatgtcatca
     3001 gccttgagta taaaccagaa ttggaaattg cctttgacga agaggatttg cttgaaaatc
     3061 cagaattggc agagatgatg tctgagtttg atgcctatat tggggaattg ttcatctcta
     3121 tcgataaggc tcatgagcag gccgaagaat atggtcacag ctttgagcgt gagatgggct
     3181 tcttggcagt acacggcttt ttacatatta acggctatga tcactacact ccggaagaag
     3241 aagcggagat gttcggttta caagaagaaa ttttgacagc ctatggactc acaagacaat
     3301 aaacgaaaat ggaaaaatcg tgacctgata tccagtttag aatttgccat cacagggatt
     3361 tttactgcta tcaaggaaga acgaaatatg cgaaaacatg cagtgacggc tctagtggtc
     3421 atccttgcag gttttgtttt tcaggtgtca cgaatcgaat ggctctttct cctattgagc
     3481 attttcttgg tagtagcctt tgagattatc aactctgcta ttgaaaatat ggtggatttg
     3541 gccagtcact atcacttttc catgctggct aaaaatgcca aggatatggc ggctggcgcg
     3601 gtattagtgg tttctctttt cgcagcctta acaggtgcat tgatttttct cccacgaatc
     3661 tgggatttat tattttaaac agtaagagga aattatgact tttaaatcag gctttgtagc
     3721 cattttagga cgtcccaatg ttgggaagtc aaccttttta aatcacgtta tggggcaaaa
     3781 gattgccatc atgagtgaca aggcgcagac aacgcgcaat aaaatcatgg gaatttacac
     3841 gactgataag gagcaaattg tctttatcga cacaccaggg attcacaaac ctaaaacagc
     3901 tctcggagat ttcatggttg agtctgccta cagtaccctt cgcgaagtgg acactgttct
     3961 tttcatggtg cctgctgatg aagcgcgtgg taagggggac gatatgatta ttgagcgtct
     4021 caaggctgcc aaggttcctg tgattttggt ggtgaataaa atcgataagg ttcatccaga
     4081 ccagctcttg tctcagattg atgacttccg taatcaaatg gactttaagg aaattgttcc
     4141 aatctcagcc cttcagggaa ataacgtgtc tcgtctagtg gatattttga gtgaaaatct
     4201 ggatgaaggt ttccaatatt tcccgtctga tcaaatcaca gaccatccag aacgtttctt
     4261 ggtttcagaa atggttcgcg agaaagtctt gcatctaact cgtgaagaga ttccgcattc
     4321 tgtagcagta gttgttgact ctatgaaacg agacgaagag acagacaagg ttcacatccg
     4381 tgcaaccatc atggtcgagc gcgatagcca aaaagggatt atcatcggta aaggtggcgc
     4441 tatgcttaag aaaatcggta gcatggcccg tcgtgatatc gaactcatgc taggagacaa
     4501 ggtcttccta gaaacctggg tcaaggtcaa gaaaaactgg cgcgataaaa agctagattt
     4561 ggctgacttt ggctataatg aaagagaata ctaagtagag gtaggctcat gcctgcttct
     4621 tgtttttaca gaaggaggac ttatgcctga attacctgag gttgaaaccg tttgtcgtgg
     4681 cttagaaaaa ttgattatag gaaagaagat ttcgagtata gaaattcgct accccaagat
     4741 gattaagacg gatttggaag agtttcaaag ggaattgcct agtcagatta tcgagtcaat
     4801 gggacgtcgt ggaaaatatt tgctttttta tctgacagac aaggtcttga tttcccattt
     4861 gcggatggag ggcaagtatt tttactatcc agaccaagga cctgaacgca agcatgccca
     4921 tgttttcttt cattttgaag atggtggcac gcttgtttat gaggatgttc gcaagtttgg
     4981 aaccatggaa ctcttggtgc ctgacctttt agacgcctac tttatttcta aaaaattagg
     5041 tcctgaacca agcgaacaag actttgattt acaggtcttt caatctgccc ttgccaagtc
     5101 caaaaagcct atcaaatccc atctcctaga ccagaccttg gtagctggac ttggcaatat
     5161 ctatgtggat gaggttctct ggcgagctca ggttcatcca gctagacctt cccagacttt
     5221 gacagcagaa gaagcgactg ccattcatga ccagaccatt gctgttttgg gccaggctgt
     5281 tgaaaaaggt ggctccacca ttcggactta taccaatgcc tttggggaag atggaagcat
     5341 gcaggacttt catcaggtct atgataagac tggtcaagaa tgtgtacgct gtggtaccat
     5401 cattgagaaa attcaactag gcggacgagg cacccacttt tgtccaaact gtcaaaggag
     5461 ggactgatga gaaaaatcat cggaatcact gggggaattg cctctggtaa gtcaactgtg
     5521 acaaattttc taagacagca aggctttcaa gtagtggatg ccgacgcagt cgtccaccaa
     5581 ctacagaaat ctggtggtcg tctgtttgag gctctagtac agcactttgg gcaagaaatc
     5641 attcttgaaa acggagaact caatcgccct ctcctagcta gtctcatctt ttcaaatcct
     5701 gaagagcaaa aatggtctaa gcaaattcaa ggggagatta tccgtgagga actggctact
     5761 ttgagagaac agttggctca gacagaagag attttcttca tggatattcc cctacttttt
     5821 gagcaggact acagcgattg gtttgctgag acttggttgg tctatgtgga ccgagatacc
     5881 caagtagaac gcttaatgaa aagggaccag ttgtccaaag atgaagctga gtctcgtctg
     5941 gcagcccagt ggcctttaga aaaaaagaaa gatttggcca gccaggttct tgataataat
     6001 ggcaatcaga accagcttct taatcaagtg catatccttc ttgagggagg taggcaagat
     6061 gacagagatt aactggaagg ataatctgcg cattgcctgg tttggtaatt ttctgacagg
     6121 agccagtatt tctttggttg taccttttat gcccatcttc gtggaaaatc taggtgtagg
     6181 gagtgagcaa gtcgcttttt atgcaggctt agcaatttct gtctctgcta tttccgcggc
     6241 gctcttttct cctatttggg gtattcttgc tgacaaatac ggccgaaaac ccatgatgat
     6301 tcgggcaggt cttgctatga ctatcactat gggaggcttg gcctttgtcc caaatatcta
     6361 ttggttaatc tttcttcgtt tactaaacgg tgtatttgca ggttttgttc ctaatgcaac
     6421 ggcactgata gccagtcagg ttccaaagga gaaatcaggc tctgccttag gtactttgtc
     6481 tacaggcgta gttgcaggta ctctaactgg tccctttatt ggtggcttta ttgcagaatt
     6541 atttggcatt cgtacagttt tcttactggt tggtagtttt ctatttttag ctgctatttt
     6601 gactatttgc tttatcaagg aagtttttca accagtagcc aaggaaaagg ccattccaac
     6661 aaaggaatta tttacctcgg ttaaatatcc ctatcttttg ctcaatctct ttttaaccag
     6721 ttttgtcatc caattttcag ctcaatcgat cggccctatt ttggctcttt atgtacgcga
     6781 cttagggcag acagagaatc ttctttttgt ctctggtttg attgtgtcca gtatgggctt
     6841 ttccagtatg atgagtgcag gagttatggg aaagctaggt gacaaggtgg gcaatcatcg
     6901 tctcttggtt gtcgcccaat tttattcagt catcatctat ctcctctgtg ccaatgcctc
     6961 tagccccctt caactaggac tctatcgatt cctctttggc ttgggaaccg gtgccttgat
     7021 tcccggggtt aatgccctac tcagcaaaat gactcccaaa gccggcattt cgagggtctt
     7081 tgccttcaat caggtattct tttatctggg aggtgttgtt ggtcccatgg caggttctgc
     7141 agtagcaggt caatttggct accatgctgt cttttatgcg acaagccttt gtgttgcctt
     7201 tagttgtctc tttaacctga ttcaatttcg aacattatta aaagtaaagg aaatctagtg
     7261 cgagtaaaaa ttaatctcaa atgctcctct tgtgacagta tcaattacct aaccagtaaa
     7321 aattcaaaaa cccatccaga caagattgag gttttaaagt attgtcccaa ggaaagaaaa
     7381 gtaaccctac atcttgaatc taagtagatt ttatggtaaa ataataggga ttttaaggag
     7441 tttgatatgt ataacctatt attaaccatt ttattagtat tatctgttgt gattgtgatt
     7501 gcaattttca tgcaaccaac caaaaaccaa tccagcaatg tatttgatgc cagttcaggt
     7561 gatttgtttg aacgcagtaa agctcgcggt tttgaagctg taatgcagcg tttgacaggg
     7621 attttagtct ttttctggct agccattgcc ttagcattga cggtattatc aagtagataa
     7681 gaaaataatg ggcaggacta ggtctttgcc tctttttatt tttaaaggat gtttgagaag
     7741 gttttacagt aaaagaaaat taaaaaatct agaaagaaaa tatgaaagat agaataaaag
     7801 aatatttaca agacaaggga aaggtgactg ttaatgattt ggctcaggct ttgggaaaag
     7861 acagttccaa ggattttcgt gagttgatta aaaccttgtc cttaatggaa agaaagcacc
     7921 aaattcgttt tgaagaagat ggcagtctga ccttggaagc caagaaaaaa catgagatta
     7981 ccctcaaggg gatttttcat gcccataaaa atggttttgg ttttgtcagc ctagaagggg
     8041 aagaggacga cctttttgta gggaaaaatg atgtcaacta tgctattgat ggtgataccg
     8101 tcgaggtagt gattaagaaa gtcgctgacc gcaataaggg aacagcagca gaagccaaaa
     8161 ttattgatat cctagaacac agtttgacaa cagttgtcgg gcaaatcgtt ctggatcagg
     8221 aaaagcctaa gtatgctggc tatattcgtt caaaaaatca gaaaatcagt caaccgattt
     8281 atgttaagaa accagcccta aagttagaag gaacagaagt tctcaaggtc tttatcgata
     8341 aatacccaag caagaaacat gatttctttg tcgcgagtgt tctcgatgtg gtgggacact
     8401 caacggatgt cggaattgat gttcttgagg tcttggaatc aatggacatt gtatccgagt
     8461 ttccagaagc tgttgtcaag gaagcagaaa gtgtgcctga tgctccgtct caaaaggata
     8521 tggaaggtcg tctggatcta agagatgaaa ttacctttac cattgacggt gcggatgcca
     8581 aggacttgga cgatgcagtg catatcaagg ctctgaaaaa tggcaatctg gagcttgggg
     8641 ttcacatcgc agatgtttcc tattatgtga ccgagggttc tgcccttgac aaggaagccc
     8701 ttaaccgtgc gacttctgtt tacgtgacag accgagtggt gccaatgctt ccagaacgac
     8761 tatcaaatgg catctgctct ctcaatcccc aagttgaccg cctgacccag tctgctatta
     8821 tggagattga taaacatggt cgtgtggtca actataccat tacacaaaca gttatcaaga
     8881 ccagttttcg tatgacctat agcgatgtca atgatatcct agctggcgat gaagaaaaga
     8941 gaaaagaata ccataaaatt gtaccaagta tcgaactcat ggccaagctt catgaaactt
     9001 tagaaaacat gcgtgtgaaa cgtggagctc tcaattttga taccaatgaa gcgaagattt
     9061 tagtggataa acaaggtaag cctgttgata tcgttcttcg gcagcgtggc attgccgagc
     9121 ggatgattga gtcttttatg ttgatggcta atgaaacagt tgccgaacat ttcagcaagt
     9181 tggatttgcc ttttatctac cgaattcacg aggagcctaa ggctgaaaag gttcagaagt
     9241 ttattgatta tgcttcgagt tttggcttgc gcatttatgg gactgccagt gagattagcc
     9301 aggaggcact tcaagacatc atgcgtgctg ttgagggaga accttatgca gatgtattgt
     9361 ccatgatgct tcttcgctct atgcagcagg ctcgttattc ggagcacaat cacggccact
     9421 atggactagc tgctgactat tatactcact ttaccagtcc aattcgtcgt tatccagacc
     9481 ttcttgttca ccgtatgatt cgggattacg gccgttctaa ggaaatagca gagcattttg
     9541 aacaagtgat tccagagatt gcgacccagt cttccaaccg tgaacgtcgt gccatagaag
     9601 ctgagcgtga agtcgaagcc atgaaaaagg ctgagtatat ggaagaatac gtgggtgaag
     9661 agtatgatgc agttgtatca agtattgtca aattcggtct ctttgtcgaa ttgccaaaca
     9721 cagttgaagg cttgattcac atcactaatc tacctgaatt ttatcatttc aatgagcgtg
     9781 atttgactct tcgtggagaa aaatcaggta tcactttccg agtgggtcag cagatccgta
     9841 tccgtgttga aagagcggat aaaatgactg gagagattga tttttcattc gtacctagtg
     9901 agtttgatgt gattgaaaaa ggcttgaaac agtctagtcg tagtggcaga gggcgtggtt
     9961 caaatcgtcg ttcggataag aaggaagaca agagaaaatc aggacgctca aatgataagc
    10021 gtaaacattc acaaaaagac aagaagaaaa aaggaaagaa acctttttac aaggaagtag
    10081 ctaagaaagg agccaagcat ggcaaagggc gagggaaagg tcgtcgcaca aaataaaaag
    10141 gcacgccacg actatacaat cgtagatacg ctagaggcag ggatggtcct gactggaact
    10201 gaaatcaaga gtgtacgagc tgctcgaatt aatctcaagg atggctttgc tcaagtgaaa
    10261 aatggagaag tttggctgag caatgttcat atcgcacctt acgaagaggg caatatctgg
    10321 aaccaggaac cagaacgtcg tcgtaaactc ctgctccata aaaagcaaat tcaaaaattg
    10381 gaacaagaga ccaaagggac aggaatgacc ttagttcccc ttaaggtcta tataaaagat
    10441 ggctacgcta agcttctttt aggacttgcc aaagggaagc atgactatga caaacgggag
    10501 tctatcaaac gtcgtgagca aaatcgagat atcgcgcgtg tgatgaaagc tgttaatcag
    10561 cgataaaaag aggaattgaa aatggaaaaa ttagttgcct ataaacgcat gcctttgtgg
    10621 aataaacaaa caatgcctga agctgttcag caaaagcaca atacaaaagt tgggacttgg
    10681 gggaaaatta ctgtcttgaa gggagctctc aagtttattg aattgacaga agaaggggaa
    10741 gttctagctg aacacctctt tgaagcaggg gcagacaatc caatggccca gcctcaagcc
    10801 tggcaccgag tggaagctgc cacagatgat gtggaatggt acttggaatt ttattgtaaa
    10861 cctgaggatt attttgctaa aaaatacaat accaaacctg ttcattcaga ggtcctagag
    10921 gccatgcaga cagtgaaaca agggaaagct ttggatttgg gttgtggtca ggggcgtaat
    10981 tctctttttc tagcccagca agattttgat gtgacggctg tagatcaaaa tggactggct
    11041 cttgaaatct tgcaaagcat tgtggagcag gaagatttgg acatacctgt tgacctttac
    11101 gatatcaatt cagctagcat tggacaagaa tatgatttta tcgtttcaac agttgttctc
    11161 atgtttctac aagcggaccg cattccagct attattcaaa atatgcagga gaaaaccagt
    11221 gttggtggtt acaaccttat cgtttgtgcc atggacacgg aggactatcc ttgctcggtt
    11281 aacttcccat tcacctttaa agaaggagaa ctggcagact attacaagga ttgggaattg
    11341 gttaagtaca atgaaaatcc aggccatttg catcgtcgcg atgagaatgg caatcgtatt
    11401 caactacgct ttgcgacctt actagctaag aaaatcaagt aaacacacat gaagattagg
    11461 aattttcctg atcttttttc ttttttacga atgatataga aaaggaggga attcatgttt
    11521 gttgcgagag atgctagggg agaattggta aatgtgttag aggataaact tgagaagcaa
    11581 gcatacacct gcccagcttg tggaggccag ctccatttgc gtcaaggacc aagtgtacgg
    11641 acgcattttg cccataaatc cttaaaagac tgtgattttt tctttgaaaa tgaaagtcca
    11701 gaacacctgg ccaataagga atccctctat cactggttaa aaaaagagac aaaggttcaa
    11761 ttagagtacc cgctttcaga acttaaacag attgcggatg tatttgtaaa tggcaatcta
    11821 gctctagaag ttcagtgtag tcccttgcct cagaaagtcc ttaaagagcg aagtgaggga
    11881 tatcgtagtc agggttacca agtactgtgg ttgctgggtc aaaaactgtg gctcaaggag
    11941 cgtttgactc gtctacagca aggttttctt tatttcagtc aaaacatggg cttttatgtt
    12001 tgggaattag acaaggaaaa acaagtttta agactcaaat acctgattta ccaggatctc
    12061 cgcggtaaac tccattatca aatcaaggaa ttttcctatg gtcaaggtag tttattggaa
    12121 atattgcgtc ttccctataa gagacaaaaa atatctcatt ttacagtttc tgaggacaag
    12181 gacatctgtc gctatatccg gcaacaactt tattatcaaa atctcttttg gatgaaagaa
    12241 caagcagaag cctatcaaaa gggagaaaat atcctgactt atggactgaa agaatggtat
    12301 ccacaaattc gaccaatagt gggcaaattt ttccagattg aacaagactt gactagctat
    12361 tatcagcact tttataccta ttaccaaaaa aatcctcaaa atgattggca aaagctttat
    12421 ccaccagcct tttatcagca atatttcttg aaaaatatgg tagaatagaa aggatggagg
    12481 aatctaatgg tattacaaag aaatgaaaga aatgaaataa atgaaaaaga tacatgggat
    12541 ctatcaacga tctacccaac tgaccaggct tgggaagaag ccttaaaaga tttaacagaa
    12601 caattggaga cagtagccca gtatgaaggc catctcttgg atagtgcgga taacctacta
    12661 gaaatcactg aattttctct tgaaatggaa cgccagatgg agaagcttta cgtttatgct
    12721 catatgaaga atgaccagga tacacgtgaa gctaagtatc aagagtacta tgccaaggcc
    12781 atgacactct acagccagtt agaccaagcc ttttcattct atgatcctga atttatggag
    12841 attagcgaaa agcagtatgc tgacttttta gaagctcaac caaagctgca ggtttatcaa
    12901 cactattttg acaagctctt gcaaggcaag gatcacgttc tttcacaacg tgaagaagaa
    12961 ttattggctg gagctggaga aatctttggt tcagcaagtg aaaccttcgc tatcttggac
    13021 aatgcggata ttgtgttccc ttatgtccta gacgatgatg gtaaagaagt tcagctatct
    13081 catgggactt acacacgttt gatggagtct aaaaaacgtg aggttcgccg tggtgcctat
    13141 caagctcttt atgcgactta cgaacaattc caacacacct atgccaaaac cttgcaaacc
    13201 aatgttaagg tgcaaaatta ccgtgctaaa gttcgtaact acaagagtgc tcgtcatgca
    13261 gccctcgcag cgaattttgt tccagaaagt gtttatgaca atttggtagc agcagttcgc
    13321 aagcatttgc cactcttaca tcgctatctt gagcttcgtt caaaaatctt ggggatttca
    13381 gatctcaaga tgtacgatgt ctacacaccg ctttcatctg ttgaatacaa ttttacctac
    13441 caagaagcct tgaaaaaagc agaagatgct ttggcagtct tgggtgagga ttacttgagc
    13501 cgtgtcaaac gtgccttcag cgagcgttgg attgatgttt acgaaaatca aggcaagcgt
    13561 tcaggtgcct actctggtgg ttcttacgat accaatgcct ttatgcttct caactggcag
    13621 gacaatctgg acaatctctt tactcttgtt catgaaacag gtcacagtat gcattcaagc
    13681 tatactcgtg aaactcagcc ttatgtttac ggagattact ctatcttttt ggctgagatt
    13741 gcctcaacta ccaatgaaaa tatcttgacg gagaaattat tggaagaagt ggaagacgac
    13801 gcaacacgct ttgctattct caataacttc ctagatggtt tccgtggaac agttttccgc
    13861 caaactcaat ttgctgagtt tgaacacgcc attcaccaag cagatcaaaa tggggaggtc
    13921 ttgacaagcg atttcctaaa taaactctac gcagacttga accaagagta ttatggtttg
    13981 agtaaggaag acaatcctga aatccaatac gagtgggctc gcattccaca cttctactat
    14041 aactactatg tatatcaata ttcaactggc tttgcggccg cctcagcctt ggctgaaaaa
    14101 attgtccatg gtagtcaaga agaccgtgac cgctatatcg actacctcaa ggcaggtaag
    14161 tcggactatc cacttaatgt catgagaaaa gctggtgttg atatggagaa ggaagactac
    14221 ctcaacgatg cctttgcagt ctttgaacgc cgtttaaatg agtttgaagc ccttgttgaa
    14281 aaattaggat tggcataaaa tggttgaatc gtatagtaag aatgctaacc ataacatgcg
    14341 tcgtcctgtc gtcaaagaag aaattgtaga cttgatgcgt cagcgtcaaa agcaggtcac
    14401 aggtttcttg aaagaattgg aagactttgc ccgcaaggaa aatattccta ttattcccca
    14461 tgaaacggtt gcttatttcc gttttcttat ggaaaccatg cagcctaaaa atattctgga
    14521 aattgggacg gctatcggtt tttcagctct cttgatggct gaacatgcgc caaatgctaa
    14581 gattacaact attgatcgta atccagaaat gattggtttt gccaaggaaa attttgccca
    14641 gtttgacagt cgcaagcaaa tcactctcct agagggagat gcggtggatg tcttatctac
    14701 actgacagag tcttatgatt tcgtctttat ggattctgcc aagtctaaat acatcgtctt
    14761 tctgccagaa atcctcaaac atttggaagt tggtggtgtg gttgtcttgg atgatatttt
    14821 tcaaggtggt gatgttgcca aggatattat ggaagtccgt cgtggtcagc gaaccattta
    14881 tcgaggcctt caaaaattat tttatgcaac cttagacaat ccagaactca ccgcaacatt
    14941 agtgccttta ggagatggta ttctcatgct tcgtaaaaat gtagcagatg ttcaactgtc
    15001 tgaaagcgaa tgattttcag aaaaatttaa gaaaaaatag taaaatagat agagtaacac
    15061 ttatctcaaa ggagtagaca tgaagaaaaa attattggca ggtgccatca cactattatc
    15121 agtagcaact ttagcagctt gttcgaaagg gtcagaaggg gcagacctta tcagcatgaa
    15181 aggggatgtc atcacagaac atcaatttta tgagcaagtg aaaaacaacc cttcagccca
    15241 acaagtcttg ttaaatatga ccatccaaaa agtttttgaa aaacaatatg gctcagagct
    15301 tgatgataaa gaggttgatg atactattgc cgaagaaaaa aaacaatatg gcgaaaacta
    15361 ccaacgtgtc ttgtcacaag caggtatgac tcttgaaaca cgtaaagctc aaattcgtac
    15421 aagtaaatta gttgagttgg cagttaagaa ggtagcagaa gctgaattga cagatgaagc
    15481 ctataagaaa gcctttgatg agtacactcc agatgtaacg gctcaaatca tccgtcttaa
    15541 taatgaagat aaggccaaag aagttctcga aaaagccaag gcagaaggtg ctgattttgc
    15601 tcaattagcc aaagataatt caactgatga aaaaacaaaa gaaaatggtg gagaaattac
    15661 ctttgattct gcttcaacag aagtacctga gcaagtcaaa aaagccgctt tcgctttaga
    15721 tgtggatggt gtttctgatg tgattacagc aactggtaca caagcttaca gtagccaata
    15781 ttacattgta aaactcacta agaaaacaga aaaatcatct aatattgatg actacaaaga
    15841 aaaattaaaa actgttatct tgactcaaaa acaaaatgat tcaacatttg ttcaaagcat
    15901 tatcggaaaa gaattgcaag cagccaatat caaggttaag gaccaagcct tccaaaatat
    15961 ctttacccaa tatatcggtg gtggagattc aagctcaagc agtagtacat caaacgaata
    16021 gtccaaatca atgagtcagg gaaaaaaact cgacttcagg aaaaaatgaa gcaaacattc
    16081 ccacaataaa acgcatagta caaggtttgt actgccccca aaagttagac aattaattta
    16141 tctgaaggat ttagttctgt attgcacagg gctaagt
//
LOCUS       DAVAAL010000034        16149 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVAAL010000034 DAVAAL010000000
VERSION     DAVAAL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16149
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.035"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            145..6846
                     /locus_tag="VQM60_000925"
     CDS             145..6846
                     /locus_tag="VQM60_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET1080810.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTANVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIEAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7094..7951)
                     /locus_tag="VQM60_000926"
                     /pseudo
     CDS             complement(7094..7951)
                     /locus_tag="VQM60_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8162..8521)
                     /locus_tag="VQM60_000927"
     CDS             complement(8162..8521)
                     /locus_tag="VQM60_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET1080811.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMVVELFLMK"
     gene            8613..9570
                     /locus_tag="VQM60_000928"
                     /pseudo
     CDS             8613..9570
                     /locus_tag="VQM60_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; frameshifted;
                     internal stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9554..10111
                     /locus_tag="VQM60_000929"
     CDS             9554..10111
                     /locus_tag="VQM60_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET1080812.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10104..10358
                     /locus_tag="VQM60_000930"
     CDS             10104..10358
                     /locus_tag="VQM60_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080813.1"
                     /translation="MDKQYLREKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10370..12424
                     /locus_tag="VQM60_000931"
     CDS             10370..12424
                     /locus_tag="VQM60_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET1080814.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHSFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12603..13463
                     /locus_tag="VQM60_000932"
     CDS             12603..13463
                     /locus_tag="VQM60_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET1080815.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLRDPVFKKD"
     gene            13674..14384
                     /gene="ccdA2"
                     /locus_tag="VQM60_000933"
     CDS             13674..14384
                     /gene="ccdA2"
                     /locus_tag="VQM60_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET1080816.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14404..14970
                     /locus_tag="VQM60_000934"
     CDS             14404..14970
                     /locus_tag="VQM60_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET1080817.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14981..16093
                     /gene="msrB"
                     /locus_tag="VQM60_000935"
     CDS             14981..16093
                     /gene="msrB"
                     /locus_tag="VQM60_000935"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET1080818.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISEDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         4888 a         3056 c         3588 g         4617 t
ORIGIN      
        1 catatctacg gcaaggcgac gctgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgctgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caagcttcaa gtcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa atgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggca ctttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgcagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 aatttcgatc atgaatctcc tgcacaaaat gaaggtggac aactcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcacaa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc tatccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac gtggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggcaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcatcagc aacccgtaca
     2101 cgaggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt cagattatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt gttcttgaat ggaaaatctc ttggtcttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccaggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc acactgacag cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaag aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtgc caaaagtaca gaccattatt ggagaggcac ctgaaatgcc taccactgtt
     3181 ccgtttgtat acagtgatgg tagccgtgca gaacgtcctg taacctggtc ttcagtagat
     3241 gtgagcaagc ctggtattgt aacggtgaaa ggtatggctg acggacgaga agtagaagct
     3301 cgtgtagaag tgattgctct taaatcagag ctaccagttg tgaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcctatgttt tgactgatgg aagtgtacaa
     3421 gagtatgaag tagatagctg ggagattgcc gaagaagata aagctaagtt agcaattcca
     3481 ggttctcgta ttcaagcgac cggttattta gaaggtcaac caattcatgc aacccttgtg
     3541 gtagaagaag gcaatcctgc agcacctgta gtgccaactg ttactgttgg aggtgaagca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ttgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggccc tcttcaaacc
     3781 tatgcaattc aattccttga agaagcgcca aaaattgctc acttgagttt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgat aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctgaac
     4021 gctgaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagcgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtata
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaagg tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag attcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtagtaatcc agaagcttca gtcggtgttc tgtttggaga ttcaggtatc
     4741 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtta aagcagataa caagcgtgga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa acaaggacaa
     5101 acaagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caaatgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
     5461 ctgacagttg aatgggaaga agttccagcg gaaaatctga caaaagcagg tcaatttact
     5521 gttcgaggcc gtgtccttgg tagtgacctt gttgctgagg tcactgtacg agtgacagac
     5581 aaacttggtg aggctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtcac caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgcg
     5821 caagccaaac ttcacttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacattg aagccggtga tgaaattaac gttaccttta aagctgtcaa agccaaagcc
     6061 atgagatggc gtatggagcg taaagcagat aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac cgagtgaatt gcctcaagaa agcactcaat cgaagattct tgtagatgga
     6181 aaagagcttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaataat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agttaaggaa
     6361 taccgtatcc agttgactaa ggaaaaacca gtttctgaga agacagttgc tgctgtacaa
     6421 gaagatcttc caaaactcga atttgttgaa aaagatttgg cctacaagac agttgagaaa
     6481 aaagattcaa cactgtatct aggtgaaact cgtgtagaac aagaaggaaa aactggtaaa
     6541 gaacgtatct ttacagcgat taatcctgat ggaagtaagg aagaaaaact ccgtgaagtg
     6601 gtagaagctc cgacagaccg catcgtcttg gttggaacca aaccagtagc tcaagaagct
     6661 aaaaaaccac aagtgtcaga aaaagcagat acaaaaccaa ttgattcaag tgaagctagt
     6721 caaactaata aagcccagtt accaaataca ggtagtgcgg caagccaagc agcagtagca
     6781 gcaggtttag ctcttctagg tttgagtgca ggattagtag ttactaaagg taaaaaagaa
     6841 gactagaaat tttgatacct tctttatcat taaagaccaa agaaaaactc ttgtcctaaa
     6901 tagaagataa agagtctggg acaaaatagt tctcaaccac aaaaaagcta gatatttcca
     6961 attgcggaac tctagctttt taattttgag tcgttctctt attgtatagt agattgaaac
     7021 tagagtagta cacttctact tctaaaacat tgttagaaat cgatttgact gtccttattg
     7081 atttgtcctg ttcttatttc atttcactat gttttaggag gttattggcc ataagtacca
     7141 atcccatgtc aattctcact tgacgcttgc ctctcagatt acatctcttg taacccaaac
     7201 aagcctttaa ctgcccaaag acaggttcca catcaatctt gcgttgagcg aaaatctgtc
     7261 taccttgggg agataaaagc gcctgacatt ctttagcttt caagttttga tagcgttcgt
     7321 tcatatatag cccccttttg aggggctgat tcaggttcgt cggcatagta aaccttgatt
     7381 tcctgttgaa agtctgtctg tgttttctga tgtttggtat ggtgaaaacg ataataccag
     7441 ccatcaggat gtgtgtagct atcctccttg tcattatagt gccaattcgc taagttttta
     7501 gctgactgtt tataccctct cttttgttcc ttatcaaaca tggcatattt aatcagaagg
     7561 ggagtgattt cttcttgaaa atagacttgt atctgttctt gaagtttggc ggagaatttc
     7621 tctgtcgttt tcttccacac aaaactatac ttgttggcat tggattcaat cttagttccg
     7681 tcaataaaca aacaatctaa ggtcactaac tcttccattt ttcaattttt cgaccagaga
     7741 aaatcccttg tgaatagaca aatagaagag tagatacaag cattttaggg tgataagacg
     7801 ggcgatcaaa ggcatgatag aaggcgtaga agtgatgttc ctccaaggta ttcaccactt
     7861 tttcaatagt aaagacgaga tggtcttgtg gtaagaagga actgatttct agtggtaaag
     7921 ttgtttgatt tgtgttatag tgaatatgca tgggatagcc tttctaaatg gtttattgtg
     7981 caattctatt ttaccaagac tatcgcaacc atgcaaaaaa gcaacggcaa atccgttgct
     8041 ttttttggag ataagagact attgtcccag actcttttct ttgtataaaa ttgtatagtg
     8101 ttattgtcta tactattttg tagagaaagc aaaaatccct agaaaatgct tagggattcg
     8161 actatttcat taagaacaat tcaacaacca tctctatata catgataagg gttaataaaa
     8221 atcctgtacg ccagaagaat ttgatgaatt taggatagta aaagctgcgt tttttcaaaa
     8281 ggaagaaaaa gacgagaata atggctagga tcgagagggc tagccccagt ctagggagga
     8341 aattatgggt aaaggtttta gctgtaatca ggtaatattc aaataccaag accggaaaag
     8401 ccaaatccgc aaagtttcgt cctagttttt ttaatctaaa aagtttggtt aggataatgc
     8461 agacgactaa ggtcagtatc aataataaaa tagatgctaa tttcattaaa atcataccca
     8521 tattgtatca taaaaaaagg ctaatggaaa cgagaaacag aggaatttat aggaaagtca
     8581 ggcttttgag attgtgggtg atttttgtta taatgaaagt tataaaatct tataatacct
     8641 tgaatgatta ttatcgaaaa ctctttggag aaaagacttt taaagtccct attgatgcgg
     8701 gatttgactg tcccaatcgt gatggaactg tggctcatgg aggctgtact ttttgtacgg
     8761 tttcaggttc tggagatact attgtagccc cagatgcgcc tatccgtgag caattttata
     8821 aggaaattgg ctttatgcac cgtaagtggc cggatgttca gaagtatctg gtttattttc
     8881 aaaattttac caacacccat gaaaaggtgg aagttattcg agagcgttat gaacaggcta
     8941 tcaatgagcc aggtgtggta ggaatcaata ttggaacgcg cccagactgt ttaccagacg
     9001 aaaccatcga atatttggct gagttatcgg agtgcatgca tgtgacggtt gaattaggct
     9061 tgcagaccac ttatgaagca acctctgacc tgattaaccg tgtgcattct tatgaattgt
     9121 aggtggaaac ggtcaagcgt ttgagaaagt atccaaaaat tgagattgtt tccccatttg
     9181 atcaatggtt tgcctggtga aacccatgag atgatggttg aaaatgtccg ccgttgtgtc
     9241 acggataacg atattcaagg gattaaactg catttgctcc atcttatgac aaatactcgt
     9301 atgcaacgag attaccatga gggacgttta caattgatga gtcaggacga atatgtcagg
     9361 gtcatctgtg accaaatgga aattattccc aaacatatcg tcatccatcg aattacagga
     9421 gatgcgccta gggatatgct gattgggctt atgtggagcc tcaataaatg ggaagctctc
     9481 aacagcattg agatggagat gcgacgtcgt ggaagtgttc aaggatgcaa ggctgtaaaa
     9541 caggagtttg aaaatgaaaa gaccacttga gatggcacat gattttttgg ctgaggtcgt
     9601 gacaaaagag gatgtcgtag tggatgcgac tatgggaaat ggtcatgaca cgcttttttt
     9661 agccaagcta gccaagcaag tctatgcctt tgatattcag aagcaagcct tggaaaagac
     9721 ccaagagcgt ttgcatcagg ctggcttgac aaatgcccag ttaatcttgc aaggccatga
     9781 gacactggac cagtttgtga caaaagctaa ggcagggatt tttaatctgg gctatttgcc
     9841 gtcagctgat aagtctgtca tcacccgacc gcagacaacg attgaggcat tagaaaagct
     9901 atgtggctta cttgtcaaag gtggacgaat tgctattatg atttactatg gtcatgaagg
     9961 aggcgacctc gagagagatg ctgtcttgga ttttgtaatc cagttgaacc aacaagagta
    10021 cacagctgcc atttaccgaa ctttaaatca agtcaacaac ccgccgtttt tagtgatgat
    10081 tgaaaaatta gagagatata gacatggata aacaatacct acgtgaaaag ctggatgcca
    10141 tgcgccagaa ttttgttgaa tcaacccacc acgaacgagc ggtgggcgtg ctagaccaag
    10201 cgcatatgag caaaaaaatg cttaaaatca agaaaaaatt agttgctctt gaaatggaac
    10261 ggtgccagag aaaaattgag cacaaagact gttccaagat tgaccaaaaa atcaaagagc
    10321 agaaggagat atttgaatcc tgttgtaaaa aagattaagg aggacgtgcg tggaattact
    10381 gatttacctc atcctatttt tactggtctt gattgtctca agtacaacca ataagctcct
    10441 gccctttttg cctctccctt tggtgcagat tcttttggga attgtgattg gtctcttttt
    10501 acccaatact gactttcatc ttaatacgga gttgtttttg gccctggtta tcggaccctt
    10561 gcttttccga gaggctgaag aagcagatgt tacggctatt ttaaaacact ggcgaatcat
    10621 tgtttatctc atatttccag tgatttttat ctcgaccctg agtttgggtg gcttggccca
    10681 tcttctttgg ttcagccttc ccttggcagc ttgcttggct gttggggcag cccttggtcc
    10741 tacggacttg gtggcctttg cctctctttc ggagcgtttt agctttccta agcgcgtgtc
    10801 caatattctt aagggcgaag gactcttgaa tgatgcttct ggtttggtgg cttttcaggt
    10861 agctttgaca gcttggacaa ctggagcttt ttctctgggg caagctagca gttcgctcat
    10921 cttttcaatc ctaggcggtt ttttaattgg ctttttaaca gccatgacca atcgtttcct
    10981 ccatagcttc ttgctaagtg tgagagcaac ggatattgct agtgagcttt tattagaatt
    11041 gagtttgcct ctagtgacct tctttctggc agaagaagtc catgtttcag ggattattgc
    11101 cgtcgtagtt gctggaattt taaaggcaag tcgcttcaag aaaatcacgc tcctcgaagc
    11161 ccaagtggat acggtgaccg agacggtctg gcatacagtg acctttatgc tcaacggttc
    11221 tgtctttgtg attttaggga tggagttgga aatgatagca gaacctatct tgaccaatcc
    11281 aatctataat cctctacttt tattgctatc tctcatcgcc cttacctttg tcctctttgt
    11341 cattcgtttt attatgatct atggctatta tgcctataga acccgacgcc tcaagaaaaa
    11401 gctaaataag tatatgaagg acatgtttct cttgaccttt tcaggtgtta agggaacggt
    11461 gtccattgct acgattctct tgataccaag taatctagaa caggagtatc ctctcttgct
    11521 tttccttgtt gcaggtgtga cgcttgtcag ctttttaaca ggtctcttgg tcttgcctca
    11581 tctttctgat gaagaggaag aaagcaagga ttatctcatg catatcgcca ttttgaatga
    11641 agtaacgcta gagttggaaa aagagttgga agacactaga aataaacttc ccctctatgc
    11701 ggctattgac aattatcatg gacgtattga aaatctcatt ttaagccaag aaaaccagga
    11761 tgatcaagaa gactgggctg ctttgaaact cttgattctt agtattgaaa gtgatggttt
    11821 ggaacaggcc tatgaagagg ggaacattag caatcgtgct taccgagttt accaacgtta
    11881 tctgaaaaat atagaacaag gaatcaatcg taaacttgcc tcaagactga cctattattt
    11941 tcttgtttcc ttgaggattt tacgttttct ccttcatgaa gtttttactc tcggaaagac
    12001 cttccgtagc tggaagaaca aggagcaaag ccgtctccgt gctcttgatt atgaccaaat
    12061 tgcagagctc tatcttgcca atacagagat gattattgaa agtttggaaa acctgaaggg
    12121 agtctacaga cgctctttga ttagttttat gcaggagtct cgtcttcgag aaacagctat
    12181 tatcagcagt ggtgcctttg tcgaacgggt tatcaatcgt gtcaaaccca acaatatcga
    12241 tgaaatgctg agaggctatt atctggagcg caagttgatt ttcgaatacg aagaaaaacg
    12301 attaattaca actaagtatg ccaagaaatt acgacaaaat gtaaataact tagagaacta
    12361 ttccttgaag gaagctgcca ataccctgcc gtatgatatg gtggaattgg taagaagaaa
    12421 ttagttaata ctcttcgaaa atctcttcaa accacgtcag cgtcgccttg gattatatat
    12481 gtgactgact tcgtcagttt catctacaac ctcaaagcag ggctttgagc aacctgcggc
    12541 tagcttccta gtttgctctt tgattttcat tgagtataag attataaatg aaggagtatg
    12601 acatgaaaaa gtggtggaaa gagctgatag acaaaccttt attaaaagct tttttgcatt
    12661 attatcaagc atcagatagt gagttgacca gtgtcgcagt agcctactat tggttgattt
    12721 cgattttccc cctgcttttg gtggtggtca atatcctccc ctattttcag attcctgtgg
    12781 gagaatttct gtgctttatg aaagacgttc tgcccccaag cctctatgaa ggtgtggaaa
    12841 aaatagccag agaagttttg acgcaacctt caacaggttt attgagtttt tctgttttat
    12901 cggcgttatg gagtttttca aaatctatga atttcttgca aaaagctttt aacaaggctt
    12961 atggcgtcga aaagagtcgt ggacttattt cccatcagat gctaagtctc ctagtcagtt
    13021 ttggcttgca gctcctcttt gcgtttgcct tgtttttgat cttatttggt cagatgattt
    13081 tggctttact tgctcattat tggacaaaag atggcattat ctatcaggct ttacaagggt
    13141 tggcaggtcc tctgatttac gccctcctct ttgctatctt ggtcatgctt tattattttc
    13201 ttcccaatat atctaacagg aaaattagct ataccctacc aggcagtgcc tttgttctct
    13261 tagtgatttt aggtttacta actctgttct ctagttatct caattactat gtccatcatt
    13321 tggtggatgt ccgaatttta ggttccgttc ttcttgttgt tatgatgttt tggtttatct
    13381 tgattgctaa aattgttatc ctaggcgcgg ttatcaatgc cagtatgcag agtttgagag
    13441 atccggtctt taaaaaagac taactattta tccattacaa aagcgcatag tatcaaaact
    13501 ttatagaacc ttgatactat gcgcttttgt gctttgacag ttttttgtat agtttttgtc
    13561 caagatttct aaatcatctg attaaattcc tattttcccc tatcattgtc cctgtttttt
    13621 cgaaaatgga aggagtataa taaatctatc aataaaatga taggaggaac tttatgggtc
    13681 atattttctt ttttctaagt gtctttttgg cagggattct atccttcttt tctccttgta
    13741 tcttaccttt gttaccggtc tatacaggag tgttactaga tgataaggat ggtgctcagg
    13801 cttctagcgg caaattttca atctcagtta ctagtttatt acgaactctg gcctttatag
    13861 caggaatttc ctttatattt attttgttgg gctatggagc tggtttttta ggcgatttgc
    13921 tttatgcttc ttggttccaa tatcttactg gggcaattat tatccttctt ggtttgcacc
    13981 aaatggagat tctacacttt aaggggcttt ataaggaaaa gaggctacaa ctgcaaggac
    14041 aggggcaaaa tggtaagggc tatagtcagg catttttatt gggcttgacc tttagttttg
    14101 cttggacgcc ttgcgtgggg ccggttctgg ggtctgtttt ggccttggcg gcttcaggtg
    14161 gttcaggagc ttggcaggga gctggtctca tgttggtgta tacgctgggc ttggcgctac
    14221 cattcttgct tctagctctg acctctagtt atgttttgaa acatttccga aaacttcatc
    14281 cctatctcgg aatcctcaaa aaagtgggtg gttttctcat tattgtgatg ggactcttgg
    14341 ttctgtttgg aaatgcttca attttaagtc aattatttga ataaaatgga aaggaatatc
    14401 aatatgaaaa aatggcaaac atgtgttctt ggagcaggtt cgctcctttg tttgacggct
    14461 tgttcaggca agtccgtgac tagtgaacac caaacgaaag atgaaatgaa gacggagcag
    14521 acagctagta aaacaagcgc agctaaaggg aaagaggtgg ctgattttga attgatggga
    14581 gtagatggca agacctaccg tttatctgat tacaagggca agaaagtcta tctcaaattc
    14641 tgggcttctt ggtgttccat ctgtctggct agtcttccag atacggatga gattgctaaa
    14701 gaagctggtg atgactatgt ggtcttgaca gtagtgtcac caggacataa gggagagcaa
    14761 tctgaagcgg actttaagaa ttggtataag ggattggatt ataaaaatct cccagtccta
    14821 gttgacccat caggcaaact tttggaaact tatggtgtcc gttcttaccc aacccaagcc
    14881 tttatagaca aagaaggcaa gctggtcaaa acacatccag gattcatgga aaaagatgca
    14941 attttgcaaa ctttgaagga attagcctag gaggtgtctt atgaatgata agttaaaaat
    15001 cttcttgttg ctaggagtat tttttctagc cataaccggt ttctatgttc tattgatacg
    15061 aaatgcaggg cagacagatg cctcgcaaat tgaaaaggcg gcagttagcc aaggaggaaa
    15121 agcagtgaaa aaaacagaaa ttagtgaaga cgcagacttg cacgaaattt atctagctgg
    15181 aggttgtttc tggggagtgg aggaatattt ctcacgcgtt cccggggtga cggatgccgt
    15241 ttcaggctat gcaaatggta gaggagaaac aaccaagtac gaattaatta accaaacagg
    15301 tcatgcagaa accgtccatg tcacctatga tgccaagcaa atttctctca aggaaatcct
    15361 gcttcactat ttccgcatta tcaatccaac cagcaaaaat aaacaaggaa atgatgtggg
    15421 gacccagtac cgtactggtg tttattacac agatgacaag gatttggaag tgattaacca
    15481 agtctttgat gaggtggcta agaaatacga tcaacctcta gcagttgaaa aggaaaactt
    15541 gaagaatttt gtggtggctg aggattacca tcaagactat ctcaagaaaa atccaaatgg
    15601 ctactgccat atcaatgtta atcaggcggc ctatcctgtc attgatgcca gcaaatatcc
    15661 aaaaccaagt gatgaggaat tgaaaaagac cctgtcacct gaggagtatg cagttaccca
    15721 ggaaaatcaa acagaacgag ctttctcaaa ccgttactgg gataaatttg aatccggtat
    15781 ctatgtggat atagcaactg gggaacctct cttttcatca aaagacaaat ttgagtctgg
    15841 ttgtggctgg cctagtttta cccaacccat cagtccagat gttgtcacct acaaggaaga
    15901 taagtcctac aatatgacgc gtatggaagt gcggagccga gtaggagatt ctcaccttgg
    15961 gcatgtcttt acggatggtc cacaggacaa gggcggctta cgttactgta tcaatagcct
    16021 ctctatccgc tttattccca aagaccaaat ggaagaaaaa ggctacgctt atttactaga
    16081 ttatgttgat taatactctt cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgta
    16141 tgtagggtt
//
LOCUS       DAVAAL010000035        15120 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVAAL010000035 DAVAAL010000000
VERSION     DAVAAL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15120
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.036"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            80..718
                     /locus_tag="VQM60_000936"
     CDS             80..718
                     /locus_tag="VQM60_000936"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET1080819.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKSVLFDGMGLAK"
     gene            715..1605
                     /locus_tag="VQM60_000937"
     CDS             715..1605
                     /locus_tag="VQM60_000937"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET1080820.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            1645..1962
                     /gene="yabA"
                     /locus_tag="VQM60_000938"
     CDS             1645..1962
                     /gene="yabA"
                     /locus_tag="VQM60_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET1080821.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            1965..2843
                     /gene="rsmI"
                     /locus_tag="VQM60_000939"
     CDS             1965..2843
                     /gene="rsmI"
                     /locus_tag="VQM60_000939"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET1080822.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     SETSLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQHGMKKNQAIKEVAKYYQWNKS
                     QLYAAYHEWEENQEND"
     gene            3030..4364
                     /gene="trmFO"
                     /locus_tag="VQM60_000940"
     CDS             3030..4364
                     /gene="trmFO"
                     /locus_tag="VQM60_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET1080823.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            4448..5185
                     /locus_tag="VQM60_000941"
     CDS             4448..5185
                     /locus_tag="VQM60_000941"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET1080824.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            5194..5751
                     /gene="frr"
                     /locus_tag="VQM60_000942"
     CDS             5194..5751
                     /gene="frr"
                     /locus_tag="VQM60_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET1080825.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            5811..6665
                     /gene="cvfB"
                     /locus_tag="VQM60_000943"
     CDS             5811..6665
                     /gene="cvfB"
                     /locus_tag="VQM60_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET1080826.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDLTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            6674..6889
                     /locus_tag="VQM60_000944"
     CDS             6674..6889
                     /locus_tag="VQM60_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET1080827.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDAIWQEYLEH"
     gene            6975..7943
                     /locus_tag="VQM60_000945"
     CDS             6975..7943
                     /locus_tag="VQM60_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET1080828.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEETACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(7921..8100)
                     /locus_tag="VQM60_000946"
     CDS             complement(7921..8100)
                     /locus_tag="VQM60_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080829.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            8136..8636
                     /locus_tag="VQM60_000947"
     CDS             8136..8636
                     /locus_tag="VQM60_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1080830.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNCRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            8639..8899
                     /locus_tag="VQM60_000948"
                     /pseudo
     CDS             8639..8899
                     /locus_tag="VQM60_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(9266..9592)
                     /locus_tag="VQM60_000949"
     CDS             complement(9266..9592)
                     /locus_tag="VQM60_000949"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080831.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(9538..9951)
                     /locus_tag="VQM60_000950"
     CDS             complement(9538..9951)
                     /locus_tag="VQM60_000950"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET1080832.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(9952..10377)
                     /locus_tag="VQM60_000951"
     CDS             complement(9952..10377)
                     /locus_tag="VQM60_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET1080833.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVADRMAV"
     gene            10554..11123
                     /locus_tag="VQM60_000952"
     CDS             10554..11123
                     /locus_tag="VQM60_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1080834.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            11191..11841
                     /locus_tag="VQM60_000953"
     CDS             11191..11841
                     /locus_tag="VQM60_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HET1080835.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEEEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            11825..14065
                     /locus_tag="VQM60_000954"
     CDS             11825..14065
                     /locus_tag="VQM60_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET1080836.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKKIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GYLNTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLIGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGWNSW
                     KIESVR"
     gene            14207..14431
                     /locus_tag="VQM60_000955"
     CDS             14207..14431
                     /locus_tag="VQM60_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080837.1"
                     /translation="MIIGLFQSSISAVTATKSYKYDWNTVLEYSINYHDHQYAWIPEW
                     SRYYSYSEYKVGGGWNYARYEVINYYSGGY"
     gene            14464..15051
                     /locus_tag="VQM60_000956"
     CDS             14464..15051
                     /locus_tag="VQM60_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080838.1"
                     /translation="MLQLTHVTLKTRQVILQDADFTFKKGRFYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
BASE COUNT         4514 a         2831 c         3511 g         4264 t
ORIGIN      
        1 aaatgagaag acaaaaagat tgtcacaaac ccctattttt ttgatagaat agaagtagta
       61 aaaaagaaat gagttagaca tgtcaaaagg atttttagtc tctcttgagg gaccagaggg
      121 agcaggcaag accagtgttt tagaggctct gctaccaatt ttagaggaaa aaggagtaga
      181 ggtgttgacg acccgtgaac ctggcggagt cttgattggg gagaagattc gggaagtgat
      241 tttggatcca agtcatactc agatggatgc taaaacagag ctacttctct atattgccag
      301 tcgcagacag catttggtgg aaaaagttct tccagccctt gaagctggca agttggtcat
      361 catggatcgt tttatcgata gttctgttgc ctatcaggga tttggtcgtg gcttagatat
      421 tgaagccatt gactggctca atcagtttgc gacagatggc ctcaaacccg atttgacact
      481 ctattttgac atcgaggtgg aagaagggct ggctcgtatt gctgctaata gtgaccgcga
      541 ggttaatcgt ttggatttgg aagggttgga cttgcataaa aaagttcgtc aaggctacct
      601 ttctcttctg gataaagagg gaaatcgcat tgtcaagatt gatgctagtc tccctttgga
      661 gcaagttgtg gaaactacca agtctgtctt gtttgacgga atgggcttgg ccaaatgaaa
      721 caagatcaac taaaggcttg gcaaccagct cagtttgacc gttttgtccg tatcttagaa
      781 caagaccagc tcaatcacgc ctatctcttt tcaggtttct ttggaagctt ggaaatagcg
      841 caatttttag ctaagagcct cttttgtacg gataaagttg gcgtcttacc atgtgagaaa
      901 tgccgaagtt gcaagctgat tgaacaggaa gagtttccag atgtcacctt gattaagcca
      961 gtcaatcagg tcatcaagac agaacgcatt cgggaattgg tgggacagtt ttctcaagca
     1021 gggattgaaa gccagcaaca ggtctttatc atcgagcaag cggataaaat gcatcccaac
     1081 gcagccaatt ctctgctcaa ggtcatcgaa gaaccccaga gtgaagttta tattttcttc
     1141 ttgactagcg atgaggaaaa gatgttaccg acaatccgaa gtcggaccca gatcttccac
     1201 tttaaaaagc aagaagaaaa acttatctta ctcttagaac aaatgggact tgttaagaaa
     1261 aaagcgactc ttttagctaa gtttagtcaa tcgcgagctg aagcagaaaa gttggctaat
     1321 caggcaagtt tttggacctt ggtcgatgaa agtgaacgcc tgctgacttg gttagtagct
     1381 aagaaaaaag aaagttatct acaggttgcc aaattagcca acttggcaga tgataaggaa
     1441 aaacaggatc aggttttacg gattcttgaa gttctctgtg ggcaggacct cttgcaggta
     1501 agagtaagag tgattctaca agatttacta gaagctagaa aaatgtggca agctaatgtc
     1561 agctttcaaa atgccatgga atatctggtc ttgaaagaaa tataaactca aaaatgaatg
     1621 ataaagaaag gaaagggctg ttttatggac aaaaaagaat tatttgacgc gctggatgat
     1681 ttttcccaac aattattggt aaccttagcc gatgtggaag ccatcaagaa aaatctcaag
     1741 agcttggtag aagaaaatac agctcttcgc ttggaaaata gtaagttgcg agaacgcttg
     1801 ggtgaggtgg aagcagacgc tcccgtcaag gccaagcatg ttcgcgaaag tgtccgtcgt
     1861 atttaccgtg atggatttca cgtatgtaat gatttttatg gacaacgtcg agagcaggac
     1921 gaagaatgta tgttctgtga tgagttgtta tacagggagt aagcatgcaa attcaaaaaa
     1981 gttttaaggg gcagtctccc tatggcaagc tgtatctagt ggcaacgccg attggcaatc
     2041 tagatgatat gacttttcgt gctatccaga ccttgaaaga agtggactgg attgctgctg
     2101 aggatacgcg caatacaggg cttttgctca agcattttga catttccacc aagcagatca
     2161 gttttcatga gcacaatgcc aaggaaaaaa ttcctgattt gattggtttc ttgaaagcag
     2221 ggcaaagtat tgctcaggtc tctgatgccg gtttgcctag catttcagac cctggtcatg
     2281 atttggttaa ggcagctatt gaggaagaaa ttgcagttgt tacagttcca ggtgcctctg
     2341 caggaatttc cgccttgatt gccagtggtt tagcgccgca gccacatatc ttttacggct
     2401 ttttaccgag aaaatcaggc cagcaaaagc aatttttcga ctcgaaaaaa gactatcctg
     2461 aaactcagat tttttatgaa tcgccccatc gtgtggcaga tacattggaa aatatgctag
     2521 aagtctacgg tgaccgctcg gtagtcttgg tcagggaatt gactaaaatc tatgaagaat
     2581 accaaagagg taccatttct gaattgctgg aaagcatctc tgaaacgtct ctcaagggtg
     2641 aatgccttct tattgttgaa ggtgccagtc aggatgtgga ggaaaaagac gaggaggact
     2701 tgttcttaga aatccaagcc cgcatccaac atggtatgaa gaaaaatcaa gccattaagg
     2761 aagttgcgaa atactaccag tggaataaaa gtcagcttta tgctgcctac cacgaatggg
     2821 aagaaaatca agaaaatgac taaacaggga agtttgcctt cttccctttt tttgttatac
     2881 tagtagaaga aaaaaataga aagatttgtg ggagtgaaaa agtcctgtgg actttttcag
     2941 cctgagccaa gaaactcgaa agctcgtaag tctgattggc tttttcaatg tgaatcttaa
     3001 cttcacactc ccaaagaggt attagtgtcg tgtctcaatc ttatatcaat gttatcggtg
     3061 ctggtttggc aggttctgaa gcagcttacc aaatcgcaga gcgtggtatt ccagttaaac
     3121 tttatgaaat gcgtggtgtc aagtcgactc ctcagcataa aacagacaat tttgctgagt
     3181 tggtttgttc caattctttg cgtggggatg ctttgacaaa tgcagttggt cttctcaagg
     3241 aagaaatgcg tcgcttgggt tctgttatct tggaatctgc tgaggctaca cgtgttcctg
     3301 ccggtggagc ccttgcggtg gatcgtgatg gtttctctca aatggtgacc gaaaaagtgg
     3361 tcaaccaccc cttgattgaa gtggtgcgtg atgaaattac agaattgcca acagatgtta
     3421 ttacagttgt ggcgactggt cccttgacta gcgatgccct ggctgaaaag attcatgccc
     3481 ttaataatgg cgatggcttc tatttctacg atgcagcagc gcctattatc gatgtcaaca
     3541 ctatcgatat gagtaaggtc tatctcaaat ctcgttatga taagggagaa gcagcctatc
     3601 tcaatgctcc aatgaccaag caagagttta tggatttcca tgaagccttg gtcaatgcgg
     3661 aagaagcacc gctcaattct tttgaaaaag aaaagtactt tgaaggatgt atgccaatcg
     3721 aagttatggc caaacgtggc attaaaacta tgctttatgg ccctatgaag ccagtcggtc
     3781 ttgagtatcc ggacgactac acaggacctc gtgatggaga atttaaaaca ccttatgcgg
     3841 ttgtgcaact tcgtcaggat aatgcagctg gtagcctata caatattgtt ggtttccaga
     3901 cccacctcaa atggggagaa caaaagcgtg tcttccaaat gattccgggt cttgaaaatg
     3961 cggagtttgt ccgttatggt gtcatgcatc gcaattctta catggattca ccaaatcttc
     4021 ttgagcagac ttaccgttcc aagaaacaac caaatctctt ctttgctggt cagatgacgg
     4081 gggtggaagg ctatgttgag tcggcggctt cgggcttagt tgcgggaatt aacgcagctc
     4141 gtctcttcaa ggaagaaagc gaggttattt tccccgagac gacagcgatt ggaagcttag
     4201 ctcattacat tacccatgcc gacagcaaac atttccaacc aatgaatgtc aattttggga
     4261 tcatcaagga gttggaaggc gagcgtatcc gtgataagaa agctcgttat gaaaaaattg
     4321 cagagcgtgc ccttgccgac ttagaggaat ttttgactgt ctaatttttt tgaaagaatt
     4381 gttcatgata ctataaaaat cttagaaatt gtgataaaat aggtaggatg aaagaaggag
     4441 agtgaaaatg gcgaatccca agtataaacg tattttaatc aagttatcag gtgaagccct
     4501 tgccggtgaa cgtggcgtag ggattgatat ccaaacagtt caaacaatcg caaaagagat
     4561 tcaagaagtt catagcttag gtatcgaaat tgcccttgtt attggtggag gaaatctctg
     4621 gcgtggagaa cctgcagcag aagcaggtat ggaccgtgtt caggcagatt acactggaat
     4681 gcttgggact gttatgaatg ctcttgtgat ggcagattca ttgcaacaag ttggggttga
     4741 tacgcgtgta caaacagcta ttgccatgca acaagtggca gagccttatg tccgtggacg
     4801 tgcccttcgt caccttgaaa aaggccgtat cgttatcttt ggtgctggaa ttggttcacc
     4861 atacttctcg acagatacaa cagcggccct tcgtgcagct gaaatcgaag cagatgccat
     4921 cctcatggct aaaaatggcg tcgatggtgt gtacaatgcc gatcctaaga aggacaagac
     4981 agccgttaag tttgaagaat tgacccaccg tgatgttatc aacaaaggtc ttcgtatcat
     5041 ggactcaaca gcctcaaccc tctcaatgga caacgacatt gacttggttg tcttcaacat
     5101 gaaccaatca ggcaacatca aacgtgtcgt atttggtgaa aatatcggaa caacagtttc
     5161 aaataatatc gaagaaaagg aataagaaag aatatggcta acgtaattat tgaaaaagct
     5221 aaagagagaa tgacccagtc tcaccaatca cttgctcgtg aatttggtgg tatccgtgct
     5281 ggtcgtgcca atgcaagctt gcttgaccgt gtacatgtag aatactatgg agtcgaaact
     5341 cctcttaacc aaatcgcttc aattacgatt ccagaagcgc gtgttttgtt ggtaacacca
     5401 tttgacaagt cttcattgaa agacatcgaa cgtgccttga acgcttctga tcttggtatc
     5461 acaccggcta atgacggttc tgtgattcgc ttggttatcc cagctcttac agaagaaact
     5521 cgtcgtgacc ttgctaaaga agtgaagaag gtcggcgaaa atgctaaagt ggctgtccgc
     5581 aatatccgtc gcgatgctat ggacgaagct aagaaacaag aaaaagcaaa agaaatcact
     5641 gaagacgaat tgaagactct tgaaaaagat attcaaaaag taacagacga tgctgttaaa
     5701 cacatcgacg acatgactgc taacaaagag aaagaacttt tggaagtcta aaaataaaca
     5761 gaaaaactca gttggcattg ctggctgagt tttattcgaa agaaggaaat atgaatacaa
     5821 atcttgcaag ttttatcgtt ggactgatca tcgatgaaaa tgaccgtttt tactttgtgc
     5881 aaaaggatgg tcaaacctat gctcttgcta aggaagaggg ccaacataca gtaggggata
     5941 cggtcaaagg ttttgcatac acggatatga agcaaaaact ccgcctgaca accttagaag
     6001 tgactgccac tcaggaccaa tttggttggg gacgtgtcac agaggttcgt aaggacttgg
     6061 gtgtctttgt ggatacaggc cttcctgaca aggaaatcgt tgtgtcactc gatattctcc
     6121 ctgagctcaa ggaactctgg cctaagaagg gcgaccaact ctacatccgt cttgaagtgg
     6181 ataagaaaga ccgtatctgg ggcctcttgg cttatcaaga agactttcaa cgtctcgctc
     6241 gtcctgctta caacaacatg cagaaccaaa actggccagc cattgtttac cgtctcaagc
     6301 tgtcaggaac ttttgtttac ctaccagaaa ataatatgct tggttttatt catcctagcg
     6361 agcgttacgc agagccacgt ttggggcaag tattagatgc gcgcgttatt ggtttccgtg
     6421 aagtggacct cactctgaac ctctccctca aaccacgctc ctttgaaatg ttggaaaacg
     6481 atgctcagat gattttgact tatttggaaa gcaatggcgg tttcatgacc ttaaatgata
     6541 agtcatctcc agacgacatc aaggcaacct ttggcatttc taaaggtcag ttcaagaaag
     6601 ctttaggtgg tcttatgaag gctggtaaaa tcaagcaaga ccagtttggg acagagttga
     6661 tttagggaga tttatgagaa aatcatttta cacttggctc atgaccgagc gcaatcctaa
     6721 aagtaacagt cccaaagcta tcttggcaga cctcgctttt gaagaggcag cttttccaaa
     6781 acacacagat gattttgatg aggtcagtcg ctttttggag gagcatgcca gtttctcttt
     6841 taacctagga gactttgacg ctatctggca agaatactta gaacactagc atttattcac
     6901 taggtttggg ctagtaattt ctccatccct ctgctataat aaaaagaaat aaaaggatta
     6961 gagaggttct ttatttgaag gaacattcaa tagacattca actgagtcat ccagatgact
     7021 tgtttcatct ttttggttcc aatgaacgcc atcttcgttt gatggaagaa gagctggatg
     7081 tggtgattca tgctcgtacg gagattgtcc aggttttggg agaagagact gcctgtgagg
     7141 aagcccgtca ggttatccaa gctttgatgg tcttggtgaa ccgtgggatg accgttggta
     7201 cgccagatgt ggtgactgca attagcatgg tcaaaaacga tgaaattgat aagtttgtcg
     7261 ccctttacga agaagaaatt atcaaggata atactgggaa accgatccgc gttaaaacgt
     7321 taggtcaaaa actttatgtg gacagtgtca aacagcatga tgtgaccttt ggaattgggc
     7381 ctgcagggac ggggaagacc tttcttgcag ttactttggc agtgactgcc cttaaacgtg
     7441 ggcaagtcaa gcggattatc ttgacacgtc cagcggtgga agcaggagag agtctaggtt
     7501 ttcttccggg tgatcttaag gagaaggtag atccttacct tagacctgtt tatgatgcct
     7561 tgtatcagat tctggggaaa gaccaaacaa ctcgactcat ggagcgtgaa attatcgaga
     7621 ttgcgcccct tgcctatatg cgtggacgga ccttggatga tgcctttgtc attctcgatg
     7681 aggcgcaaaa tacgaccatc atgcagatga agatgttctt gactcgttta ggttttcatt
     7741 ctaagatgat tgtcaatgga gatattagtc agattgacct gccacgtaat gtcaagtccg
     7801 gtttgattga tgctcaagag aaactcaaga acatccatca gattgacttt gttcattttt
     7861 cagccaagga tgtggttcgc catccagttg tcgctcagat tatccgagcc tatgaatatt
     7921 ctactgaagt tgcacacgac tgattttgag gaagttcgcc tgcaaaagaa tatacttttg
     7981 agagaggaaa atccagttgt ataggctaaa ggttttatcc aaaggtttga gacaacgatt
     8041 aggcacgatg gaaagaactt ttatgtggct gatgacgatc agtgcatctt cctgtgtcat
     8101 aatcacaggg cacaagaaag taggaatttg aaaagatgat tgaccaacta tctaagtatt
     8161 acagttgtag gatactaact gaaaaggata ttccaagtat tttatcttta tatgaaagta
     8221 atcctctgta ttttcagcat tgtccaccag agccaaattt tgcaactgta aaagaggaca
     8281 tgctttgtct acctgaaggt aaagctaagg ctgataagtt ttttgttgga ttttggaatg
     8341 gatctgacct tgtggctgtt atggattttg tctatgcata tcctgatgag gagactgttt
     8401 ttattggttt gtttatggtt gatcaagcct atcagaggaa agggattggt agtcatattg
     8461 tgacagaagc actagcttat tttgctaaga actgtcgaaa ggcacgtttg gcttatgtta
     8521 agggaaatcc gcaatctcag catttttggg aaaagcaggg ctttaaatca attggatgcg
     8581 aggttaagca agaactctat acggttgtta tcgctgaaca gagcctagaa gattagaaat
     8641 ggcatcaagt aagaactatt tggaatttgt tttggaacaa ttatcaggat tagatgatgt
     8701 gacttaccgt tccatgatgg gggagtatat tctttacttc cgcggcaaga ttattggcgg
     8761 catttatgac gatcgctttt tagttaaacc cgtgcaagca gtcttagata agattgacca
     8821 atcttctttt gagtttccat acaaaggtgc caaagaaatg atttgagtgg aagaacttga
     8881 taataagatg tttctataag acctgatttt agctatgtat aaccaactgc caacgcccaa
     8941 acctaaaaag aaaaagcaag ggtgaacgaa gtaaaaaaga agtctgctaa ggccctgtct
     9001 ttgcacgggt aaaattttat atataaaaag aagctgggac taaagagctc agcttccttt
     9061 ggtttatata attgtcatta caagacgaag tggttgggcg aaactctgtt gattttattc
     9121 aatttagagt ttcttatgca caattgagtc tggaacgaaa gtctccagtt gcaaagtata
     9181 cagtacaata aaccaacgat gtaatagctg atgacacaaa gcacagtggg taggacttgc
     9241 gaagtcaccc ttttcttttc aaaatttata ctaaatcatt gatatcagtg tagtcacgat
     9301 taagtccttg agcaactggt aggctagtta agtaaccttg ataagtggtc acaccttgac
     9361 gcaagccttc atcttcagag attgcttgtg cgaatccttt gccagccaaa gcttcgatat
     9421 aaggaagagt gacattggtt agggcgatgg ttgaagtgcg agcaaccgca ccagggatat
     9481 tggcaacggc atagtggaga acaccgtgtt tttcatagac gggttcatcg tgcgttgtca
     9541 cacggtcagc tgtttcgata acgccacctt ggtcaacagc aacgtcaacg atacagagcc
     9601 tggacgcatt tgtttgacca tctcatctgt caccaattcc ggtgcttttg caccagggat
     9661 gagaatggct ccaatcacca catcagcatc tctcacactt gcttcaatgt tgaatgaatt
     9721 agatataaga gtttgaattt gacttccaaa gacttcttct agaactgaga gacgcttggc
     9781 actaatatct aaaatagtca cttgagcacc aagaccaagg gcgatgcggg cagcatgtgt
     9841 accgacgaca ccatcaccga tgatagttac ttttcctttt ggaacacctg gtacaccacc
     9901 aggtagaaca ccagatccac cagcttgctt agtaaggaag tgagctccga tttaaacagc
     9961 catacgatct gcaacctcac tcataggaac gaggagcggt agttgtcctt gattgtcacg
    10021 aacagtttca taggcaattc ctgttgtttt tgctgctaac atagcatctg ctaattctgg
    10081 agcagcggcc atgtgcaagt aggtgaagag aagaagatcg tcgcgcaagt aaccgtattc
    10141 agaacttaaa ggttctttta ctttcacaac caactctgct gcccaagctt caccagcagt
    10201 agcgacaatc tcagctcctt gcttttgata gtcagcatca gtaaagccag aaccgagacc
    10261 agcatttgtt tcgataagga cacgatgacc acgactaact aagctatgaa cacctgcagg
    10321 tgtgagggcg acacggtttt cgttattttt aatttctttt gggattccga ttaacattga
    10381 gataacctac ctttcaattg acggtcttgt tttggttgtc acattccagt tcataaatca
    10441 aaaatgtgac ggtttcattg tatatgaaac cgcttcaaaa atcaagaaaa acttgtcatc
    10501 caaatttttt atgctagact agtgaaaatc aagctttgat tggttggaaa agtatggaat
    10561 caatttttgt aaaacttgcc cagtatccgt ctatagaaac ggagcgttta ttgcttagac
    10621 ctgtaacttt ggatgatgcg gaagcaatgt ttgactatgc ctcggacaag ggtaatacac
    10681 gttacacttt tccaaccaat caaagcttgg aagaaaccaa gaataacatt gctcagttct
    10741 atttggctaa tcccttggga cgttggggaa tagaactaaa aagcaatggt cagtttattg
    10801 gaaccattga cttgcacaag attgattctg ttcttaagaa ggcagctatt ggctacatta
    10861 tcaataaaaa gtattggaat caaggattaa cgacagaagc caatcgtgct gtgattgagc
    10921 tagcttttga gaagataggg atgaataagt tgactgccct tcacgataag gacaatcctg
    10981 cgtcaggaaa ggtcatggag aaatcaggca tgcgtttttc ccatgcagaa ccatatgctt
    11041 gtatggacca gcatgaaaaa ggccgaatcg tgacaagagt tcattatgtc ttgaccaagg
    11101 aagactattt tgcaaataaa taagcagttg aaaagaaatt tttcgactgt tttttcttcc
    11161 tcttacgaat aatctaagag aggagaaaat atggaagcaa ttatcgagaa aatcaaagag
    11221 tataaaatca tcgtcatctg tactggtctg ggcttgcttg taggaggatt tttcctgcta
    11281 aaaccagctc cacaaacacc tgtcaaagag acgaatttgc aggctgaagt cgcagctgtt
    11341 tccaaggatt tggtatccga agaggaagtg aacaaggaag aaaaggaaga accccttgaa
    11401 caagatctaa tcacagtaga tgtcaaaggt gctgtcaaat cgccagggat ttatgacttg
    11461 cctgtaggta gtcgagtcaa tgatgctgtt cagaaggctg gtggcttgac agagcaagca
    11521 gacagcaagt cgctcaatct agctcagaaa gttagtgatg aggctctggt ttacgttcct
    11581 actaagggag aagaagcagt tagccaacag actggtttgg ggacagcttc ttcaataagc
    11641 aaggaaaaga aggtcaatct caacaaggcc agtctggaag aactcaagca ggtcaaggga
    11701 ctgggaggaa aacgagctca ggacattatc gaccatcgtg aggcaaatgg caagttcaag
    11761 tcagtagacg agctcaagaa ggtctctggc attggtggca aaacaataga aaagcttaaa
    11821 gactatgtta cagtggatta agaatttctc tattccccta atttacctga gttttctatt
    11881 actttggctt tattacgcta ttttctcagc atcttatctt gctttgttgg gctttgtttt
    11941 tctgctagtc tgtctcttta tccaatttcc gtggaaatct gctggtaaag ttctaataat
    12001 ttgcggaatc tttggatttt ggtttgtttt tcaaaattgg caacagagtc aagcgagtca
    12061 aaatctggcg gattctgttg aaagggtacg gattttacct gatactatta aggttaatgg
    12121 tgatagtcta tcctttcgtg gcaagtctaa cggtcgtgct ttccaagtct attataaact
    12181 ccagtccgag gaggagaaag aagcctttca agctttaacc gacctgcatg agataggact
    12241 agaagggaag ctttcggagc cagaagggca gagaaatttt ggtggcttta attaccaagc
    12301 ctatctgaag actcagggaa tttaccagac tctcaatatc aaaaaaatcc agtcacttca
    12361 aaagattggc agttgggata taggagaaaa cttgtccagt ttacgtcgaa aggctgtggt
    12421 ttggattaag acgcactttc cagaccctat gcgcaattac atgacaggac tcttgctggg
    12481 atatctgaac accgactttg aggagatgaa tgagctttat tccagtctag gaattatcca
    12541 cctctttgcc ctatctggca tgcaggtagg ttttttcatg aatggattta agaaacttct
    12601 cttgcgattg ggcttgaccc aagaaaagtt gaaatggctg acttatccct tttcccttat
    12661 ctatgcggga ctaattggat tttcagcatc ggttattcgc agtctcttgc aaaagctact
    12721 ggctcaacat ggggttaagg gcttggataa ttttgccttg acggtgcttg tcctctttat
    12781 tgtcatgcca aactttttct tgacagcagg aggagtcttg tcctgcgctt atgcttttat
    12841 cctgaccatg accagcaaag aaggggaggg gctcaaggct gttgctagtg aaagtctagt
    12901 catctccttg ggcatattgc ccattctatc cttctatttt gcggaatttc aaccttggtc
    12961 tatccttttg acctttgtct tttcctttct atttgacttg accttcttac cgctcttgtc
    13021 tattttattt gtcctttcct ttctctatcc agtcattcag ctgaacttta tctttgaatg
    13081 gttagagggc attattcgct tggtctcgca ggtggcaagg agaccgcttg tctttggtca
    13141 acccaacgca tggcttttaa tcttattgtt aatttccttg gctttggtct atgatttgag
    13201 gaaaaacatt aaaggattaa cagtattgag tttattgatt acaggtctct ttttccttac
    13261 caagtatcca ctggaaaatg aaatcaccat gctggatgtg gggcaaggag aaagtatttt
    13321 cctacgggat gtaactggga aaaccattct catagatgta ggtggtaagg cagaatctta
    13381 taagaaaatc gaaaaatggc aagaaaagat gacgaccagc aatgcacaga gaagcttgat
    13441 accctatctt aaaagtcgag gagtagctaa gattgaccag ctgattttga caaatacgga
    13501 caaggaacat gtcggagatt tgttagaggt gaccaaggct ttccatgtag gggagatttt
    13561 agtatcaaaa ggcagtttga agcagaagca atttgtggta gaactacagg cgactcaaac
    13621 aaaggtgcgt agtatgacag taggggagaa cttgcccatt tttggaagtc agttagaagt
    13681 tctatcccca aggaaaatgg gagatggagg acacgatgat accctagttc tgtatgggaa
    13741 attcttggat aagcaatttc tcttcacggg aaatttggag gagaaaggag agaaggactt
    13801 gctgaagcac tatccagact tgaaagtaaa tgttttgaaa gctagccaac atggcaataa
    13861 aaaatcatca agtccagcct ttctagaaaa actcaaacca gagcttactc ttatctcagt
    13921 tggaaagagc aatcgaatga aactccccca tcaggaaaca ttgacacgac tggaaggtat
    13981 caatagcaaa gtttaccgaa ctgaccagca aggagctata cggtttaaag gttggaatag
    14041 ttggaaaatc gaaagtgttc gataggaaag ataaatgttg tagattagtg aaataaacta
    14101 aaaatttgtt gcataataat gataaaaatg gtataatgaa aacgtattca atactgagga
    14161 tataaaatca ttaaaaatta gcaaaaatcg tttaattagt tagtttatga ttattggtct
    14221 ttttcagtcc agtatatctg ctgtgacagc cactaaaagt tacaagtatg attggaatac
    14281 ggttttggaa tatagtatca actatcacga ccatcagtat gcttggattc cggaatggtc
    14341 tcgttattac agctattctg agtataaagt tggcggaggc tggaactacg ctcgttatga
    14401 ggtcataaac tattacagcg gaggctatta attcttaaag agtgagaaaa aggagggcta
    14461 gatatgttgc agcttactca tgtgacctta aaaacgcgac aagtcatctt gcaagatgcg
    14521 gatttcacct ttaaaaaggg taggttttat ggtcttcttg ctatcaatgg ctctggaaag
    14581 acgaccctgt tccgtgccat tagcaattta attcccataa gtagtggaaa tatcgcagtt
    14641 cctccttctt tgttttatta tgagagcgtt gaatggctgg atggaaactt aagtgggatg
    14701 gactaccttc gtctcataaa aaacatctgg aagtcagacc taaacttgag agatgaaatc
    14761 gcctactggg aaatggctga ctatatcagt cttcccatcc gcaagtattc cttaggcatg
    14821 aagcaacgct tggtgattgc tatgtatttt ctcagtcagg ccaaatgctg gctcatggat
    14881 gagattacaa atggcttaga cgagtattat cgacagaagt tttttgatag gctagcacaa
    14941 atcgatagac aagaacagct ggttctttta agttcccact ataaggaaga gttggttgat
    15001 atctgcgata gagtagtaac cattcatcag gggcagatag aagaggttta gtttatgaaa
    15061 gatgttagtc tatttttatt gaaaaaagtt ttcaaaagtc gtttaaactg gattatctta
//
LOCUS       DAVAAL010000036        15114 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVAAL010000036 DAVAAL010000000
VERSION     DAVAAL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15114
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.037"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(28..825)
                     /gene="proC"
                     /locus_tag="VQM60_000957"
     CDS             complement(28..825)
                     /gene="proC"
                     /locus_tag="VQM60_000957"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET1080839.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(829..2091)
                     /locus_tag="VQM60_000958"
     CDS             complement(829..2091)
                     /locus_tag="VQM60_000958"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET1080840.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(2101..3231)
                     /locus_tag="VQM60_000959"
     CDS             complement(2101..3231)
                     /locus_tag="VQM60_000959"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET1080841.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(3311..5194)
                     /gene="cbpE"
                     /locus_tag="VQM60_000960"
                     /gene_synonym="pce"
     CDS             complement(3311..5194)
                     /gene="cbpE"
                     /locus_tag="VQM60_000960"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET1080842.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIELYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(5197..6084)
                     /locus_tag="VQM60_000961"
     CDS             complement(5197..6084)
                     /locus_tag="VQM60_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET1080843.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(6074..6535)
                     /locus_tag="VQM60_000962"
     CDS             complement(6074..6535)
                     /locus_tag="VQM60_000962"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET1080844.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(6532..7440)
                     /locus_tag="VQM60_000963"
     CDS             complement(6532..7440)
                     /locus_tag="VQM60_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET1080845.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            7433..7630
                     /locus_tag="VQM60_000964"
     CDS             7433..7630
                     /locus_tag="VQM60_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1080846.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(7682..8353)
                     /locus_tag="VQM60_000965"
     CDS             complement(7682..8353)
                     /locus_tag="VQM60_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET1080847.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(8407..8628)
                     /locus_tag="VQM60_000966"
     CDS             complement(8407..8628)
                     /locus_tag="VQM60_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET1080848.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            8900..9709
                     /locus_tag="VQM60_000967"
     CDS             8900..9709
                     /locus_tag="VQM60_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET1080849.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(9890..10765)
                     /gene="aguB"
                     /locus_tag="VQM60_000968"
     CDS             complement(9890..10765)
                     /gene="aguB"
                     /locus_tag="VQM60_000968"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET1080850.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(10775..11860)
                     /gene="aguA"
                     /locus_tag="VQM60_000969"
     CDS             complement(10775..11860)
                     /gene="aguA"
                     /locus_tag="VQM60_000969"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET1080851.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLYEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEVLEKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGSAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVAIDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(11857..12984)
                     /gene="nspC"
                     /locus_tag="VQM60_000970"
     CDS             complement(11857..12984)
                     /gene="nspC"
                     /locus_tag="VQM60_000970"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET1080852.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEVGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(12984..14243)
                     /locus_tag="VQM60_000971"
     CDS             complement(12984..14243)
                     /locus_tag="VQM60_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET1080853.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(14240..15100)
                     /gene="speE"
                     /locus_tag="VQM60_000972"
     CDS             complement(14240..15100)
                     /gene="speE"
                     /locus_tag="VQM60_000972"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET1080854.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
BASE COUNT         4419 a         3499 c         2759 g         4437 t
ORIGIN      
        1 cataaaagag gcagataaag ctacctctta tttacctagt tcttgtgttc gtttataggc
       61 ttgatgaact gcctccatga ctgtgcctcg aaaagcatgc gcttctaggc ttgctacgcc
      121 agcaatagtc gaaccgcctg gactacaaac ttgatctttc aaaactccag gatgttgctg
      181 gctttctagg accaattgcc cagctcctac cacagtttgg gctgccattt tcaaggctgt
      241 ttctcgtggt aatcccgtct gaacacctgc atctgccaag gcctcaataa agagatagac
      301 aaaggctggt ccacaacctg caagacctgt cgctgcattg attaagcttt ctcctaattc
      361 aaccaagaga ccagccttgg ctaaaagctg acaaaagatc tcactgtcct cagccctgca
      421 attaggagac aaggcataac taatcactcc ttgcccgata gaagcagggg tattaggcat
      481 catacgaata attcggtgtt ggcttgggat aagacttgct agtttttcta aggtcaatcc
      541 agctgccatc gaaatcaaaa gaagactttc tcttttttca aggatggtct gatattgaga
      601 aagcagttca gaaaactgag caggcttaac tcctagaaaa atcacatctg cttctgcaaa
      661 catttcttca ttgctggaag cctgaccacc aaagtctgca atgaaagcat ctaccttagc
      721 ttgactacga ttggcaagga gaatctcatc tgacgtccta gtctgcaaga cagattttgc
      781 caagctagca cccatattcc ccaaaccgat aaatccaatc ttcatctctt actcccttat
      841 ctgcccatca ccagcaacca catacttgta gctggtcaac tctttcaagc ccattggacc
      901 acgcgcgtgc aatttctgag tagaaatccc catttcacaa ccaagaccaa attgtcctcc
      961 atctgtgaaa cgagttgagg cattaacata caccgctgca gagtccactt gatctgtaaa
     1021 gtatgctgca gcttcagcat tttccgtcac aatagcatcc gaatgatggg tactgtggga
     1081 ttcaatatgc gcaaccgctt cttctaaact gctcacaacc ttaacagcta gaatatagtc
     1141 taaaaactcg gtatcaaagt cttgtgcttg agcagcttga cctgaaacaa actggcttgc
     1201 tttgctatct aggcggaatt gaattggttc caacccagct tcttttcgat ctgcaaccag
     1261 catttgatcc aagcgaggaa ggatgcttgc tgccttgttt tcatgaacca gcagaacctc
     1321 catggcatta caaacagaag gacgactggt tttagcattg ttgatgatag acagcgcctt
     1381 gtcttcgtct gcatccttat ccacatagac atggacaatc ccagtccctg tctcgataac
     1441 aggtacaatc gcattctcaa ccactgcatt gatcaagcca gctcctccac gaggaatgag
     1501 aaggtctaga tagcccttgg ccttcatcat agcataacta ctttcacggc tagtatcctc
     1561 caccagttga atcacatttg gatgaatagt agtcgtctcc aagcccttct tcaaggctgt
     1621 gacaatggca tgggttgttt gataggcatc cttaccacta cgaagaacaa ccgcatttcc
     1681 actcttaaga gtcaaagcag ccgcatcaga cgtcacattt ggacggcttt catagataat
     1741 accgatgaca cccatagcta cacgtttttt tgtgataacc aaaccatttt caagctgact
     1801 tgtttctaaa acttcaccga ttggatctgg taaggcaacc acttcacgaa ttcctcttgc
     1861 catcgcttct atacgatctg catccaaata aagacgatcc aacatcacat ctgagatttt
     1921 ccccttagcc gctgccatat cgagggcatt agccgctaaa atttcctcag tagcagccac
     1981 taagtgatca gccatggcta gcaaggcttg gtttttcact tcttcactag ctgtgttaat
     2041 cgatttttta acagcctgta cctgttcaaa ttgttcttgt ctactcacca tagtttacct
     2101 ctaaaattct gtaaaaagta gttggatttc aggagtaatg gaaatccagt cgtcacggta
     2161 aatcaagaca cccttggctt tttgagaacg caacatatcc tccaaagcag atgctccaaa
     2221 ttgcacgcgt ccttttccaa gtgattttcc actttccttg tcaaatactg tcacgatatc
     2281 accgtaagaa aaggctcctt ctgcttcaac gataccagat aagagaagac tctttccatg
     2341 ttgagagaga gcttccgcag cccctttatc aacccaaata gaaccttgac tctgagcata
     2401 gaaggcaagc cattgtttct gggtacgaag ccccttctct tgagcaacaa agtaagaacc
     2461 atcctcggtc tcctctgccg cctcaatcat ggaatctgat ttcaaggatg agcagatata
     2521 aacaggaact cctgattccg tcgcgatagt tgcagccttg attttggtta acataccccc
     2581 agttccgttt gacgaacctg ctccaccagc catatcaata atctcacgat tgatggtctc
     2641 gattctctcc aagcgtttgg ctcttggatc tgaattagga tttccagtat agagaccgtc
     2701 cacatctgtc aagaaaacta aaaggtctgc ttggaccatc gccgctactt gagcacttag
     2761 agtgtcattg tccccaacct tgagctcatc aataacgaca ctatcattct cattgatgat
     2821 aggaattgcc ccacggttga gcaaaaccga caaagcctga tgggcatttt tataacgacg
     2881 cttatccaca aagtcatctt gggtcagcaa gatttgtgca gaaacgattt gacgcaagag
     2941 aagattggtc gtatattctt ccaacaaaag tccctgccct accgctgctg aagcctgttt
     3001 atcagcaatt ttagtcggac gctttttaaa tcctaaggct ccaaaaccag ccgcaatggc
     3061 acctgaagac accaaaatca actcatgacc agcctcgtgc agcatagcca actgctgggt
     3121 aatatccttt accttactac gtgataaact tccatcctca tttgtcagag aagaagtacc
     3181 caccttaaag acaatccgtt tgtatttcat agtctcactc cgattattca tatttatcca
     3241 ttataacatg aataacagga aatagaaaag agttgatagg aaaaagaagg ccctaaaacc
     3301 ttcttttcta ctactgttct gattccgatt tgtttacact cgtttcagag ctttgtgaag
     3361 ttgaggatgt caaatcttga ctagttgaag aatttgtaac actttcatgt tgactcgttt
     3421 ctttattttc aagatccttt ttcactactt ctttggattc ttttatttct gaatgacttg
     3481 tagttttaat tgtagcagat ttctcgatcg gaacatccac taaccaggca ccacttgaat
     3541 ccacagtata gccttctggt gtcttaccat tcacaagtaa ttgaccattt tctttcaaga
     3601 agtaccattt atccttgtct ttaatccaac ctatagctcg tgaaccatct gtcttgtaga
     3661 aataccaatc atttgccttt ttaagccagc cttgcttcat agctcctgat tcttctagat
     3721 agtaatgatg tccagcaact tctatctcac ctgtcttcat gataccagta gaatccatat
     3781 agtaccaggc ttctttatct tttacccagc ccgttttcat ttcccctgat ggagcaaagt
     3841 aataccattg ccccttatat tgaagccaac ctgtcttcat agagccatca acatccaaat
     3901 aataccactg ctctttatct tgaatccaac caatttccat ctggttttct ttgttaaaat
     3961 aataccagat tccagcgatt ttcttccaat ttttagcagc aacaccagag tctgtcaaat
     4021 agaaccaacg attgttccat tttttccatt gattctgtaa taagataccc gtttggttaa
     4081 agtaatacca ttcaccctta atttcattcc aaccgacagc atattctcct gtagaatcag
     4141 gcgcttgata ccaccagttt ccatatgaac tcttatgcca accagcttga aaacttggaa
     4201 tcggcttgta ggaagttgaa atgtttacta aaccgtcttg tcgaatatca aaaactgttg
     4261 catcatagtc tttgctggct gcgttgattc tctcaattcc tcgttctttg agccaattaa
     4321 catactcact atcaacacca tttttccaag gtagactatc cgaagtttga acaatcaaac
     4381 tcggactcaa atttttaatg aaatccttgg tatttgattt gttggtatca tggtgatgat
     4441 taaacttcat caaatcaact tttccaatga gaggaccata cttgtcttct gctccatgaa
     4501 cattatctaa atcgccccca aggtaaattt tcttgccatt gactttcacc acgctaatca
     4561 aggaattgga attgtcatcc caaattttct ttaattcacc cgatgaatcg gtttcatttt
     4621 catagttata gagctcaata tccatgtccc caaactgaaa atgagcatcc ccttgtgtga
     4681 tattttgaat aactgaaaca cctttttctg aagcagtctg taaaacctta tcataaccgt
     4741 acagattatc ccatagacgt tcagaattag taatacgatt atcactatat tttttaagat
     4801 agactcggtc aactggatag gtagacagta attcatcaac atttccaata tgatcactgt
     4861 gggtatgggt caccaaaata aaatcaagtt tttggacacc caattccttc aaacgacgaa
     4921 agacacggtc tgttagaaca tgcttataag acgtttcaat tccttctctc catggatagc
     4981 gagaatcact tccatctggg aaatcataat cttctcctgt atccaccatg gcaaaatgtc
     5041 cattgctttc aagaataatc gcatcactgc caccttcttg aacattgata aagtggattt
     5101 tatttcctga actttcttga gcctgaacat tcccatacca tgacaaacct aaaaaagcgc
     5161 ctacaagtgc taaactagtt aatttctttt tcattcttac tttctcaatc tctccaaggt
     5221 ttccttaaaa atctctggga tatctgcttt aaattccaag gtcttacctg ttctcggatg
     5281 agtaaaacct aaagtcttgg catgaagaaa ttgtccatgt cctttcaaag tcttgcgagg
     5341 accatagacc tcatcaccag cgactgaatg gccgatataa gccatgtgga cacggatttg
     5401 atgagtgcgc cctgtctcca gttgcaactc tactaagcta taatcgccaa agcgttccaa
     5461 gacgtgaaaa cgcgtcactg caggcttccc tttagcagtt acagcctgtt tcttacggtc
     5521 tttttcactc cggccaatcg gcgcttcaat tacaccacga tcattaggca gatttccatg
     5581 aacaatcgcc caatatttgc ggagagactt tttatccttg agttcttggg caagtgctag
     5641 atgcgcatca tcgtttttag caatcatgag aagacctgac gtatccttat caatacggtg
     5701 aacaatccct ggacgcagaa ccccattgat acccgacaag tccttaatat gatacatgag
     5761 ggcatttact agggttccac tggtatgacc agcactcggg tgcacaacca ttccctgagg
     5821 tttgttaacg acagccacat cctcatcttg gtagactatt tctagcggaa gatcctcagc
     5881 cacatactct aatacctctg gttctggcac atggtaagtg acgacatcac cctcttggac
     5941 tgtgtattta gctttcttga cttgaccatt gaccaagacc tggcctgatt taatttgttc
     6001 attcgcgaga ctacgtgata attctgacaa atctgacaaa gccttatcca aacgcagacc
     6061 accagtttca attttaattt ccatttattt cctcttttag cattgcaatc aataaaataa
     6121 tcactccaac cgtcagatag ctatctgcca cattgaaaat tgcaaagttg ataaagtcaa
     6181 ggtggaacat atccacaaca aagccctgac tgaccctgtc aataaagttt ccaagaccac
     6241 ccgcgattat tagagtcaaa cccaagacca tccagaatga gtcctccatg tgtttatgta
     6301 aataccaaat ggcacctatc acgacaacca gagtaatgac agcgaataac agctgctgat
     6361 cttgtaagat agaaaaggct gcacctcgat tttgcaggta ggtcaagcta acgaaattgg
     6421 gaatccagga gcgcacttca cccagtggaa tctgctggac gatataggat ttgaccaact
     6481 gatccagccc aattaaaagc agtacaatga ctgccactat tgctcttttt ttcatgattt
     6541 cctcttttga tcaaaatatt cttgcatgac ttctacgaag agagtcccag cttgactaag
     6601 ctccacttct tcacgtttaa catagaccat gcggttatct aggttatcct tgagacgaat
     6661 aactgtaatg ccattaacac tgtcactatc taaaaatcca gaacctgtcg cataggcgtc
     6721 cgtccgctcc aaaataccat tcaaggtggc acggtctgtc acattaaaca tctgtgagct
     6781 agcgctggta tcgacaaagt tctctgaata ataaaggtac tcgtctttct cttgagtgaa
     6841 acgaaccgtt ggtaaatccg ctaaatcctc catgactaat tcctctttct gagctaaagg
     6901 atgaccctca cagagataaa tatgggtatg gaaaggaatc aattcgatga cctccagacc
     6961 taacttttca acccgttgca taatcccctt tttattttga ttgttgaggt agataatccc
     7021 aatctcacta tgcccttgcg ccacttcatc taatatttga acagtagttg attcaaaaat
     7081 acggaagttc ttatagtcag gatagcgctc tgaaaaggcc gtaatagttg gtggtaagaa
     7141 gtcatagtgc tggctagcaa cggaaaattc atctttttct tcttcaggat tggcatactg
     7201 attttgaaaa atatcaaatc ctttaaccaa ttcttgcgct ttttcataaa attccatccc
     7261 acgacgggtc aagaaagtcc ctgagctggt ccgacggaaa atcttaaagc ccaactcttt
     7321 ttccaaatca cgaacagaaa tagacagact cggctgacta acatacatct tttcagcagc
     7381 ttcacgaaaa gtaccactat tggcaatagc cacaacatag cgtaattgtt gaatgttcat
     7441 cttctacccc caacttcttt atcttttcat tataccatat tttagaagtt ttccaaaaag
     7501 gaaaaaagaa catcctattc ttcttaacta tcttcactat ctgcctttct cacgccaatc
     7561 ttattttcaa aatcaattca aaaaaataag tggctacaca ccacttcagt atcggaaaga
     7621 aaaacgttga ctatttgtga aaaaagaaaa tgccggaaaa ttccgacatt tttttagtaa
     7681 gctaacttcc tgaaaatagg agtaaccaaa catcccagaa ggcagtgata ttgatgagaa
     7741 tatgaactag tattggatag tagagatttt tagtcatgcg atacaataga gctaaaataa
     7801 gacctccact agcataaatg aaaaaagcaa gaggagttaa agcaaaattg atatgaatat
     7861 aaccgaaaat aatagcagaa agcaaaacat ctccgtacca aggtgagttt ttgaaaaagg
     7921 ttgtcataag cacacctcga taaatcaatt cctcagcaat aggggcgatg aagcaaacga
     7981 tgagcaagaa ataagggaac tcctgtctcc ccatcatttc taccgtttca ttcaaagaaa
     8041 tctgatttga agacagggat atgaaatacg aaaagaggaa gtcagacata tatgaaatga
     8101 tgtagcccag taaaaggtag atgaagtacc tcagctgcca cttgaaatga aaaattttct
     8161 ttcctgcaaa aaccagtaga tagatgaccg ctaagaaaag aactccgctc tccatcaaaa
     8221 gcagaatctg aaaaaattca cgacttgctg gtagataggg ctttgcgagg tgattgaaaa
     8281 ctctccaaaa ccaagttgat ttgtaaaaaa ttaaggacaa tgctagtaaa atttgaatag
     8341 cccgtttttt catattaaat tctctgcttt ctccacttgt ttcttcatat tactaataaa
     8401 ttttacttaa aatctcgcag cacttccttt gcaaagatga ttgcctcctc caatatatcc
     8461 taaatcatag gtgcctctgg gacaaaatcc atgaaccggc agagttaata ctggttctat
     8521 ttcccaatcg tacctaccat taggattttt accatatgga tctgctccac cttgtatcag
     8581 ctctaattct tcatttgtta gatttatatt ttttgtcatt aaaatcatga tatatttcct
     8641 ccattattct tcagattagt tgagtaatct gatactcata cctacttaca aaaaaactat
     8701 tatattaagt tggtttttta atttgtctag ttgcaacatt gacaaacata gtatagcata
     8761 tctttgcgaa atatcctctt caaatcatga attgtcatca aaacatctta aactataaaa
     8821 tcaattagtc tcaagctttc tatcaatttc ttctcaaaaa tatgctataa taatagcaaa
     8881 agataaagaa ggaagaccta tgattaaact actagccttg gatatggacg gcaccctcct
     8941 caatgaagcc aaggaaatcc cacaagctca cattactgct attcacaaag ccattgaaaa
     9001 aggtgtcaaa ctggttctct gtacgggtcg cccccttttc ggtgtcctcc cctactacaa
     9061 aaaactggag cttgacctcc agaacgaata tattattgtt aacaacggtt gttcaactca
     9121 ccagaccagt gattggagtc tagttgactg gaaagaactg agtccagctg acatcgaata
     9181 cctctatgac cttgctgaaa agagtgatgt tcagttgaca ctttttgacg agtcacatta
     9241 ttttgttctc ggtggcaaac ccaatcaagt tattgaaaat gatgctaaac tagtattttc
     9301 agacctgact gaaatttctc ttgaagaagc gactagtgga aagttccgga tgttccaagg
     9361 tatgttttta ggaacaaaag aacaaacaga cgattttgag cagcgttttg ctgaagagct
     9421 ttgccaacga ttcagtggag ttcgttcgca gcctgtcatt tatgaagcaa tgccgcttgg
     9481 aacgacaaag gctactgctc tttcacgact agctgagatt ttgaagattg attcctcaga
     9541 gattatggcc atgggcgatg ctaataacga tatcgaaatg ctccagtttg cagggcttgg
     9601 gattgcaatg ggaaatgcca gcgattatgt caaatctctt gcggatgccg ttacctcaag
     9661 caacgaagaa gacggcgttg cgcgtgctat tgagaaatat attctataaa aaagaaaagc
     9721 aaatagacag aagttactgc tatttgcttt tttgctaata ttttaaaata cggactaatc
     9781 aatagacaag aatagcgaat cataaaaatc gatttactag atgccatagt atcttatagt
     9841 tgctaataag aagttagtct agcagaatga atctctcatt tctcccacac taatctgtaa
     9901 tttgtctata catttctggt cttctatctc gaaacaagcc ccagtttagg cgttcacttg
     9961 ctcccttgtc taggtcataa gtagctaaca gaacagcttc ttcttgtctt tcagctcgtt
    10021 ctagaatagc tcctgtttca tccgtcataa aggaggaacc gtagaagtca agactggaac
    10081 tctgtccgcc attttcctca ctaggagtaa cctcctctaa accataacga ttggctgcga
    10141 tgactggaac aatattcgct gctgcgtgcc cttgcatagt acgttgccag tgaccacaac
    10201 tatctgtatc caaaattggc tctgaaccga tagctgtagg ataaaagagc aattcagcac
    10261 cattcaatgc aagacagcgc gctgtttcag ggaaccattg atcccaacag ataccgatac
    10321 caatcttagc atagcgagta ttccagacct tgaaaccagt gttaccaggc gtgaaataga
    10381 atttttcttg ataataatgg tcatctggta tgtgggtctt tcgataaacg cccagcactt
    10441 ccccatctgc atcaatgacg gcaatagagt tatacaagac attaccatct ttttcataga
    10501 aactgattgg taaaacaact tgtagttcct tagcaatcac cttaaaatgc tgaattgcag
    10561 tattttccgc tacagattgg gcatactggt agtagtcata ctgacgttcc tgacagaaat
    10621 agggatgttc aaacaactcg ggcaagagaa taatttgggc tccttgctca gcagcctgac
    10681 gtactaaacg ctctgcggtt tggatatttg ttgccacatc cttagcgcat tgcatctgaa
    10741 tggttgcaac tcttacattt ctcatctttt tctcctattc tggaatttgt tgggtgatac
    10801 agtggatatt gccaccacct aagagaatat ctctggctgg tattccgaca actttacggt
    10861 ctgggaaaca cttgctgagg atatctatgg ccacttggtc gtttacatcc tcaaactgtg
    10921 gaaccaagac agccttgttg gcgatataaa agtttacgta ggaagctgct agtcgttcac
    10981 ctgcgtatcg ctcttcttct ccttcttcat agatgtagcc tggcaaatct tcttctgtca
    11041 caacttgtcg aactgcaggg ataggcaatt tatgaatggt gaagtgacaa ccttttgcat
    11101 ctgtttcctg ttctaagagt tcgagatctg cttttgacat ggcatactgg ggatcatttt
    11161 cgtcatctgt ccaagccaaa acaagctcag cagaaccaac aaaggcagca acattatcga
    11221 cgtgttcatt ggtttcatcc tgataaatac cataaggaag ccaaataact ttttcagcac
    11281 caagactttc taataatgtg ttttcaatct cctctttagt caggttagga ttgcgaccag
    11341 gactaagcaa gcaactttca gttacgagaa tagttccttg accatcgcta tggattgcgc
    11401 ctccttccag tacaaaaggt ttagcatcat agacaggctt ttccaagacc tcagcaaaac
    11461 gactggctac ttggtcatcc tcttcataat cttgataaag accatcatag gtgcctcccc
    11521 aagcattgaa ggcccaatcc acggctaatt tcttaccttt atcattgacg agaatggttg
    11581 ggccagtatc acgcgcccag gcatcattgg tgggaatgtc taaataaaca accttgtctc
    11641 caagatagga ttgggcttca tatagatagg cctgctccac caaaagatag actctttccc
    11701 cttctgctat ggtctcgatg atctgagtaa atgctctttt agcagccttt ccttgaaaag
    11761 gccatgatcc tggtcgagtc ggccatatca tgagggtacc atgatggggt tcgtactctg
    11821 ctggcatgtg atagcctaat ttttttggac tgtccatcat gataatctcc ctttaaagtc
    11881 ttgatagcca aaagctttga gtaagctaca gtctccctgt tcgtccatga gatagagact
    11941 tggcaatcca ataccattaa aggtattatt tttgacaaaa gaataaatgg ccatgtcttg
    12001 aaaataaagt ctgtctccga tttggactgg attttcaaaa ctataatcac caatcacatc
    12061 gcccgtcaga caggtattag aagaaagtct gtaggtatgg gctttttcct gtgactcaaa
    12121 gccatttctc aaaggtggac gatagggcat ctcaagtaca tcaggcatat ggcaggtcgc
    12181 agaggcgtct aaaaccaaga tttccatacc gttttctaca atatctaata cctcagttgc
    12241 taaataaccc gcattaagcg caatggcttc accaggctcg atatagattt caagattgta
    12301 agtttttcgg atacgcttga tttctgaaat cagcaaatcc acatcgtaac cttctcttgt
    12361 aatatgatga ccaccaccca tattgagcca ttttacctca tgtaagtagg gaccaaactg
    12421 ttcttctact gctttcaaag ttgtttgtaa atcatctgct ccctgctcgc aaagggtatg
    12481 aaaatgaagt ccgtcaacca aatctagcaa atcactcgga atcttgtcta tagtaactcc
    12541 aaagcgagaa ccaggtgcac aagggtcata gagcgcgtga tctccttgag ttgaacactg
    12601 agggttgagg cgcaaaccaa cactgacacc agcctctcga caacgagccc catgcttacg
    12661 caactgtctc tctgagttaa agacgatatg gtctgttatc ccaagcaatt cctccaagtc
    12721 tgcatccttg aaagcaggcg caaatacatg gacttcacca ggaaattctt cccttgccaa
    12781 tttggcctca tagagtccac tagctgtcgt acctgataga tactggctaa tcaagggata
    12841 agttttgtag agggaatatg ccttctgggc aagcaagacc ttgcaaccga cctcttcttg
    12901 tacatattgt agaatgcggc aattagcttc taacttggcc aagtcaataa cataggctgg
    12961 tgttggtact tgttctaact tcattagtcc accatttgtg gattttcaac cacaacccat
    13021 ggcaaaccat actcattcaa agcttccatg aatggatctg gatctaactc ctcaaggtta
    13081 tacactccag cttgtttcca agttccgttc atgactaatt ttgtcccaat catggctgga
    13141 acgcctgtcg tgtatgaaat tgcttgtgac ccgacttctg catagcactc ttgatggtcg
    13201 caaacattgt agatgtagat agtcttttca acgccatctt tgacacctgt aaagatacat
    13261 ccaatattgg ttttacctac agtacgtggg ccaaggctag caggatctgg aagcaaggct
    13321 ttcaaaaatt gaattggaac aatttcttgg ccgttaaagt taatggtatc cgtacgaagg
    13381 agtccaacat tttcaagaca tttcatgtgc gtcaagtaag attgaccaaa agtcataaag
    13441 aaacgaatgc gtttgacacc tggaatgttc ttggccaatg attcgatttc ttcatggtga
    13501 aggagataca tatctttttg tccaacttga gggaaatcat actcacgctt gatagacata
    13561 gcttcgactt cgacccattt cccatcttcc cagtaagaac ctggcgcaga aacctcacgg
    13621 agattaattt ctggattaaa gttggttgca aatggataac cgtggtcacc gccattacag
    13681 tctaaaatgt cgatataatg gatttcatca aaatagtgtt tgagggcata agctgaaaag
    13741 acactagtta cacctgggtc aaaaccagaa ccaagaagag cagtcaagcc tgcttctttg
    13801 aatttctctt gataagccca ctgccatgag tagtcaaagt aggctgtaaa accaagttcc
    13861 ttacaacgtt tttcgtagat agcacgccac tcagggtctt ctgtgtcttc tgcttcgtag
    13921 ttggctgtat cgatatagtg aacacctgtt gccaaacaag catccataat ggttaaatct
    13981 tgataaggca gagctacatt caaaacagct tctggtttgt agctttcaat caaggtaatg
    14041 acctcttcta ccttgtcagc atcaagtgct gcagtttcaa ttttagtact tgttttgcct
    14101 tctagcttcg ctttcaagtc atcgcatttt gacttggtac ggctagcaat cataatctct
    14161 gtaaatgttt cgctatcttg acaaattttt gaaatagcaa cttgggcaac gcccccacaa
    14221 ccaataacta gtaaacgact catttttttc cttcctcttc ttctaaaatg tcctcaacat
    14281 acttgggcaa cataaaggct cccacgtgta agtttgcagt gtagtattct gtgaaaagct
    14341 ggcgtttttt ccaaccttcc ttgtcaaaat ctttgacagg gtggtatttt ttcgatgcaa
    14401 atccaaacaa ccaatagcca gctgggctag ttggaatatg ggcctgataa acccgactga
    14461 ttggaaaggc ttgattgacc ttgcggtgca tgcttcggca ggccgactca tcctcgtcaa
    14521 agaagggact cccatgctgg taaatcatga tgccgtcttc cttcagagct cgataactat
    14581 tgccgtagaa ttccttggta aagagtcctt ccgtatggcc aaatggatct gtcgcatcgt
    14641 tgatgataat atcgtaatca tcttcgcagt ttcgcaaaaa gcgtagccca ttttggtagt
    14701 aaatggtaac acgaggatca tctagccctg cagcaaagtc tgggaaatac tcacgacaga
    14761 cctcgaccaa catctcatcc ggttccacga tatcgatttg ttccagttca ggatagagtg
    14821 ttaatacttg ggcaacaccg ccgtcaccac ccccaataac caatactttc tttggatttg
    14881 ggtggacagc catgggaacg tgaacggtca tttcattgta gacgaaatca tccgcatctg
    14941 agaacaagac atggccattt aaaatcagta ttttcccaaa agctggcgta tccaagactt
    15001 cgatatcctg ccattcactt tttccagcgt aaagttgctt ggctgttctc agagacaatt
    15061 tgacatctgg agtatgaact tcagaaaacc ataaatccat ctatttctcc ttat
//
LOCUS       DAVAAL010000037        15085 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVAAL010000037 DAVAAL010000000
VERSION     DAVAAL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.038"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..290)
                     /gene="glyA"
                     /locus_tag="VQM60_000973"
     CDS             complement(<1..290)
                     /gene="glyA"
                     /locus_tag="VQM60_000973"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET1080855.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHS"
     gene            complement(352..783)
                     /locus_tag="VQM60_000974"
     CDS             complement(352..783)
                     /locus_tag="VQM60_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1080856.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(785..1387)
                     /locus_tag="VQM60_000975"
     CDS             complement(785..1387)
                     /locus_tag="VQM60_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET1080857.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(1371..2210)
                     /gene="prmC"
                     /locus_tag="VQM60_000976"
     CDS             complement(1371..2210)
                     /gene="prmC"
                     /locus_tag="VQM60_000976"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET1080858.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(2210..3289)
                     /gene="prfA"
                     /locus_tag="VQM60_000977"
     CDS             complement(2210..3289)
                     /gene="prfA"
                     /locus_tag="VQM60_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET1080859.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMLEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(3286..3876)
                     /locus_tag="VQM60_000978"
     CDS             complement(3286..3876)
                     /locus_tag="VQM60_000978"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET1080860.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            4000..4182
                     /locus_tag="VQM60_000979"
     CDS             4000..4182
                     /locus_tag="VQM60_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET1080861.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            4318..5691
                     /gene="mnmE"
                     /locus_tag="VQM60_000980"
     CDS             4318..5691
                     /gene="mnmE"
                     /locus_tag="VQM60_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET1080862.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(5738..6673)
                     /gene="dapA"
                     /locus_tag="VQM60_000981"
     CDS             complement(5738..6673)
                     /gene="dapA"
                     /locus_tag="VQM60_000981"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET1080863.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     YPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     IPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(6729..7805)
                     /locus_tag="VQM60_000982"
     CDS             complement(6729..7805)
                     /locus_tag="VQM60_000982"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET1080864.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(8148..8220)
                     /locus_tag="VQM60_000983"
     tRNA            complement(8148..8220)
                     /locus_tag="VQM60_000983"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8185..8187),aa:Thr,seq:ggt)
     gene            complement(8320..8829)
                     /locus_tag="VQM60_000984"
     CDS             complement(8320..8829)
                     /locus_tag="VQM60_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET1080865.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(8827..8923)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            8952..9383
                     /locus_tag="VQM60_000985"
     CDS             8952..9383
                     /locus_tag="VQM60_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET1080866.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            9465..9842
                     /gene="mscL"
                     /locus_tag="VQM60_000986"
     CDS             9465..9842
                     /gene="mscL"
                     /locus_tag="VQM60_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET1080867.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKTAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(9884..10978)
                     /gene="hemH"
                     /locus_tag="VQM60_000987"
     CDS             complement(9884..10978)
                     /gene="hemH"
                     /locus_tag="VQM60_000987"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET1080868.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESQQIRFLVIKDWYQQQSLLDFWTDEIRKILRNEVGEE
                     SFKVIFSAHSVPIFALDYGDPYIDQIFDNSRLIAEQLGLMTDQYTNTWQSESDIGIPW
                     IKPDVLEYLREQKQHPEHYIFVPISFISEHIEVLFDNDVECYDLCQELGVTYHRPPMP
                     NTDSRLIDALVATVRANEDKEFKTFLPEEETFDELAPSATTKDIMKETDDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(11056..11842)
                     /gene="srtB"
                     /locus_tag="VQM60_000988"
                     /pseudo
     CDS             complement(11056..11842)
                     /gene="srtB"
                     /locus_tag="VQM60_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
     gene            complement(11957..12955)
                     /gene="srtG1"
                     /locus_tag="VQM60_000989"
     CDS             complement(11957..12955)
                     /gene="srtG1"
                     /locus_tag="VQM60_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HET1080869.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(12973..14169)
                     /gene="pitB"
                     /locus_tag="VQM60_000990"
     CDS             complement(12973..14169)
                     /gene="pitB"
                     /locus_tag="VQM60_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HET1080870.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(14214..14765)
                     /gene="sipA"
                     /locus_tag="VQM60_000991"
     CDS             complement(14214..14765)
                     /gene="sipA"
                     /locus_tag="VQM60_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HET1080871.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLATL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFSMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(14765..>15085)
                     /locus_tag="VQM60_000992"
     CDS             complement(14765..>15085)
                     /locus_tag="VQM60_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET1080872.1"
                     /translation="TLQFTNGKATVDLKKDKTIKILDLPLNARYSIEEEASSSRGYQV
                     SYDKKEGTLDANKSATVTNNKNSVPETGIDFLSSTLVLGVVLPLGGIFFIILLGHLVV
                     NRRK"
BASE COUNT         4369 a         3243 c         2868 g         4605 t
ORIGIN      
        1 gaatgtggtt ggacattggc aaatttagca ccgaaaattt cttttgcgcg ttcaatagca
       61 agagtctcta caacgtctac tacatcagtt ccaccataat aacggcgtcc tgggtaaccc
      121 tcggcatatt tatttgtcaa gatagaccct tgagctgcca taacagcctt ggaaactacg
      181 ttttccgaag caattaactc gatattattt tgttggcgtt cttcttcttt ggcaatagca
      241 ttccagagat cagcatcata tgctttaaaa tcatctttgt caaaaatcat aggtcttctc
      301 ctttattgtg tgactagtcc attagtttga ttttacaata agaaaatcaa actaaaagat
      361 gcgaataaac cgtttctgca ttttatcaca agtatagcca actttttcat aaaatgcatg
      421 agcacccaga cgatgattgg cagaatttaa gcggataaac ccataaccac atctttttgc
      481 ttcttcttcc aacccttgta gtaaactttt accaatacct tgaccttgcg cttgaggtga
      541 aactgctaaa gctaagatat taaatcctgc tttggaatag agtgattcgt aaacttcagc
      601 gtggacatat ccaagtaaga catgattagc tgcatcctca tagccaagta ggaaatgatg
      661 ggaatcctga gacagtctag ctagttggct agccgtttcc tctggactaa aagtataacc
      721 caaagcctct tggttgatgt cacatatcgc tttcacatct gtttcttgca aatctcttag
      781 catttcaagc ctcctcaaaa gaaatctctg gcaaccgagc aagaatatct tctcgtttaa
      841 ttgcgccttg gcgtaagatt ttcaccttgt ctccagacaa atccacaata gttgaatcct
      901 gtccagttag aaaagcatcg tcttccagac ccagaacctc ttggtcaaaa tccttcagaa
      961 tttgttcaaa ggttacacca cttgcctgac ctgagatatt ggcagacggc ccaatcaagg
     1021 gacctgtctc tcgaattaaa tccagtgtga tagggtgact gggcatccga aatccaatag
     1081 ttgcaaggtc agaatttacc caatagggaa ctcggtcatt ggcttcgaga ataatggtca
     1141 agggacctgg caaaaaggtc tctacaagtt tttgtagata agttggctga ttcttagaaa
     1201 agtgcaagat atcctcgaaa gaggcgatat tgagattgag tgccttatct ctaggacgac
     1261 gtttgagttg gtaaacatgg tcaactgctt tttcatctaa ggccttggca aaaagaccat
     1321 aaacagtctc tgtaggtaga acgacagctc caccattttc caactcttgt ctaatcctgt
     1381 ccatcatcaa ccacaaccat cctattttga ccaaattggt ctttgagtgt tcgaactttt
     1441 ttttcaggaa gatgttttct aaaaagttcg ggaacacttt gaccttgctt gtatccaatt
     1501 tcaaggtaaa tcttaccacc atccttgaga tagtcttttg catcttccgc aattctacag
     1561 taaatagcta ggccatcctc gtctgcaaag agagctagat gaggctccga atgcaaaaca
     1621 ttcaagccta cctctgactc atcttcacga gagatatagg gtggattgga tacaattata
     1681 tcatattttt cagaaatttc tgcaaaacaa tcagattttt taaaaaatat attaagattt
     1741 tgatcgctag cattctctga tgcaagctct aaggcctctt gagaaacatc tgctgctgtc
     1801 actgaccaat ctggtctatt ttttgctaga gcgagagcaa tagctccact acctgttcca
     1861 atatccagaa ctgagagatt cgtctcaaga ttttcagtta gaataagctc cactaactcc
     1921 tctgtttctg gacgaggaat caaaacccgt tcatccacct ttaaatgcat tccataaaaa
     1981 tctgcctgtc caatgatgta ctgagctggc ttgtgagctg ctagttgctg gtaaatatct
     2041 tctacaaatt gtttttcttc ctctgttgtc acctcctgct ggagggcaaa aataaagtct
     2101 gtaaaagata gatttttcag actacgatag acaaaagaga ggctttccgc ttcctctcct
     2161 tgtcttatca actcttcttc aaaatttgaa aataattgag ctaatttcat tatttgttta
     2221 attcttctag tttttgtgtt tggtcataaa gcaccaaggc atccacaact tcgtccaatt
     2281 taccagacaa aatcgtatct agtttttgga gggtcaagcc gatacggtgg tctgtgacac
     2341 ggttttgtgg gaagttataa gttcggatcc gttctgaacg gtcaccagta ccgattgtcg
     2401 acttacgctc agcgtcttgt tcatcctgag caatctgagc aaagtggtca gcgacgcgtg
     2461 cacggataat cttcatagcc ttctcgcggt tcttctgctg ggtacgttct tcctgcatct
     2521 caaccttgat attggttggc aagtgaacga tacgaacggc agtcgcaacc ttattgacgt
     2581 tctgtccacc agcaccagag gcgtgataga tgtcgacacg aaggtctttt ggatcaatgt
     2641 cgtattcaac ctcttcaact tctagcataa caagaactgt cgctgtcgaa gtatgaacac
     2701 ggccttggct ttctgtcaca ggaacacgtt gcacacggtg ggcacctgat tcatacttaa
     2761 gcttagagta tacagactga cctgaaacca tagcaaccac ttctttaaaa ccaccgacac
     2821 cattcataga ggcttccatg acttcaaagc gccaaccttg ggcttccgca tacttttggt
     2881 acatagttag caaatctcca gcgaaaagtg ccgcttcgtc tccaccagct gctccacgga
     2941 tttcaaggat gatattcttg tcatcgtttg gatcctttgg aaggagcaaa attttcagtt
     3001 tttcttcata ttcttctttt tcagccttgg catctttgag ttcttgcttg gccatttctt
     3061 ccaagtccgc atctccgcct gattccttaa tcatctcttc ggcatcgacg atattttgaa
     3121 ggacttgttt atactcacgg taggctatta cggtgtcacg attggaagct tcttcttttg
     3181 aaagctccat aaaacgcttg gtgtctgaaa cgacatcagg gtcactcagc aattctccta
     3241 gttcttcata acggtcttct acagcttgta gttgatcata gatgttcatt tttcttcatt
     3301 ctccttattg atttcagggg caaaataatg tttacggcaa accgagatat aggtttcatt
     3361 accaccaatc tggatctgtt ctccatcgta aacgggcagt ccatcctgtg ttcgcaacac
     3421 catggtcgcc tttttcttgc aatactgaca tatggtcttg atttcgtcaa tcttgtctgc
     3481 taaaagcaag agatatttgg aaccttcgaa caattcattg cgaaagtcat ttttcaagcc
     3541 aaaagccatg acgggtatgt ctaactcgtc cacaacacga gctaggtcgt aaacatggtg
     3601 gcgtttgaga aactgggctt catcgaccaa aacacagtaa ggtttttctg gtaggtctcg
     3661 gatatagcca aagatatccg ttgtttcctc aatcgcaagg gcagggcgtt tcatgccaat
     3721 tcgactcgac acatagccaa caccgtcacg cgtatccaga gccgaagtca taatgacaac
     3781 accttttcct tgctcctcgt agttataggc cactttgaga atctcaatcg ttttaccaga
     3841 gttcatggtc ccataacgat aatacaactg tgccatgttt cttgcttcac gtccatttct
     3901 aaatttttgc tacattctag tatatcataa ttttcttaag ctttaaacgg caaaatgtgg
     3961 taaaatagaa gaaatcaaaa actagtggag gaagctatta tgccatttgt acgcatcgat
     4021 ttatttgaag gacgcacgct cgagcaaaag aaagctcttg ctaaggaagt aacggaagcg
     4081 gttgttcgca acactggagc ccctcaatct gctgttcatg tcatcatcaa cgacatgcca
     4141 gaaggaactt atttcccaca gggggaaatg cgcaccaaat aagctagctt aagcagaatt
     4201 gcttaggctt tttcaatctc caagtagcac ccattgaaga aatagtctaa atttgttaca
     4261 atttgaaaag agacttggaa aattccaaga aaagagcaat taattaaagg aaatattatg
     4321 attacacgtg aatttgatac catcgctgct atctctactc cactaggtga aggagctatt
     4381 ggtattgtcc gcctgagcgg aacagacagt tttgctattg cgcaaaagat ttttaaagga
     4441 aaagacttga acaaggttgc cagccacact ctcaactacg gtcacattat tgatcctctg
     4501 actggtaaag tcatggacga ggttatggtt ggggctatga agtctccaaa gaccttcact
     4561 cgtgaggata ttatcgagat taacacccac ggtggtattg cggtgaccaa tgagattctc
     4621 cagctagcta tccgtgaagg agctcggttg gcagaacctg gtgaatttac caaacgtgcc
     4681 tttttaaacg gtcgcgtaga tttgacacag gccgaggcgg tgatggatat catccgagcc
     4741 aagacagaca aggccatgaa catcgcggtc aaacaacttg atggttccct ttctgatctt
     4801 atcaataata tccgtcaaga aatccttaac acacttgccc aagttgaggt caatatcgac
     4861 tatcctgagt atgacgatgt tgaggaagcc actactgctg ttgtccgaga gaagacaatg
     4921 gagtttgagc aattactaac caagctcctt aggacagcac gtcgtggtaa aatccttcgt
     4981 gaaggaattt caacggctat cattggacgt cccaacgttg ggaaatcaag ccttctcaac
     5041 aacctcttgc gtgaggacaa ggctatcgta acagatatcg ctgggacaac acgagatgtc
     5101 atcgaagagt acgtcaacat caatggtgta cctctcaaat tgattgatac agccggtatt
     5161 cgtgaaacag atgacatagt ggaacaaatc ggtgttgagc gttcgaaaaa agccctcaag
     5221 gaagccgact tggttctact agtgctaaat gccagtgaac cactgactgc gcaagacaga
     5281 caacttcttg aaattagcca agataccaat cgcattattc tacttaataa aaccgacctg
     5341 ccagaaacga ttgaaacttc gaaactacct gaagacgtta tccgtatttc agtccttaaa
     5401 aaccaaaaca tcgataagat tgaagagcga atcaacaacc tcttctttga aaatgctggt
     5461 ttggttgagc aagatgctac ctacttgtca aacgcccgtc acatttcctt gattgagaag
     5521 gccgttgaaa gcctacaagc tgttaaccaa ggtcttgaac tagggatgcc agttgacttg
     5581 cttcaagttg acttgacccg tacttgggaa attctaggag aaatcactgg agatgctgct
     5641 ccagatgaac tcatcaccca actctttagc caattctgtt taggaaaata agaaaaatcc
     5701 atgatccttc attcggtcat ggattttatt gtctttatta gtaatctggt cttaagaccc
     5761 ctgttacagt tgccttagtt gcttcgtagt cgccatctac gacaaccttg ataatgcgtt
     5821 tggcatcttc ttctggtgct ggaataagag gtagacgagt gggtccagct tcaaatccca
     5881 tatagttaag aactgcctta actggagcag gacttggata agagaagaga gcattaacct
     5941 taggaatgaa tttacgctga attgctgcgg ctttcttcat atcgctttct gcaatggcag
     6001 taaacatctc gtgcatttca tccccatttg tatgagaggc aacagaaata accccatccg
     6061 ccccaaggtt catggcatgg aaagcatctc catcctcacc tgtataaatc aagaactctt
     6121 caggcttgtg ctcaatcaag taagccatat tagccaagct agtacattct ttgacaccga
     6181 taatatttgg atagtcagcc aagcgaagca tggtttctgg agtcaattcg acaactacac
     6241 gccctggaat gttatagata ataattggta ggtcagaagc atctgcaata gccttaaagt
     6301 gctgatacat cccttcttga gaaggtttgt tgtagtaagg aacaatagca agcccagctg
     6361 cgaaaccacc aaattccgct acttctttga caaactcaat agagtcacgc gtatcattag
     6421 tacctacacc cgcaatcaaa ggaacgcgtc cattgacaac cttttgtaca gccgcaaaca
     6481 actccaactc ctcatcgtgg gtcaaagttg gactctcagc agtcgttcct gcgagaagaa
     6541 ttccatccgt atgatgggcc aataaatgct caatcaaggc tggaatagca tcaaagttaa
     6601 tggaaccatc ctcatggaag ggggtaataa aggctgtaat gattttacat tcttttaaat
     6661 cttgataaga cacgaaaaca cctctctatt tcaaagaagg agttcatctg aactcctaaa
     6721 cgatatgact attttaattc aaatttcaat tcggctgttg gacgaaccaa tccacgttca
     6781 tgaagagttt cagcaatctg aactgagttc caagcagcac ctttgagaag gttatctgaa
     6841 acaacccaca tgtgaattcc tttttctgca tccaagtctt tacggatacg accaacaaag
     6901 gtatcacgcg aaccaactgc attgatggct tgaggataga tttgatgagc tacatcatct
     6961 tcaagaacag cacctgggaa ggctgcgata gctgctttta cttcttcgat tggagccact
     7021 tcttttgttt cgatataaac agactcagag tgagctgaca agactggaat acgcacacat
     7081 gttgcagata ctgcaatgct atcatcttcc ataattttct tagtttcctt ggtcatcttc
     7141 atctcttcgt aagtgtaatc attgtcagtg aagacatcga tttgtggaag agcattgaag
     7201 gcgataggat aatgtttctt gtcaccgcct gaaggtaaga tttccgcatg caaatcacgt
     7261 ggtttcactc catcattcaa gacctcacga agttcacgtt gtgtctcaag aattgctccc
     7321 ataccagcac ctgaaacggc ttgataagtt gaaacaatga tacggtccaa gccccatttt
     7381 tggcgaaccg gctcaagagc caccatcatt tggattgttg aacagttagg gcaggcaatg
     7441 attccgttgt gggcatcaag tgcatgagca ttgacctctg gaacaaccaa aggaacatct
     7501 ggattttgac ggaaataaga tgtattatcc actactacca cgccagcttt tactgcgtat
     7561 ggtgcatact tagctgatgt cgaaccacct gctgaaaaga gagcaatatc aactccttca
     7621 aaagctgttt cagtcgtttc ttcaatcgta atatcttgat ctttaaattt caatgactta
     7681 cctgctgaac gtgcagaagc aaggtaacgg attttatcga ttggaagtgt tgattcttcc
     7741 aacattttta tcatctgagc accgacagca cctgtcgcgc cgactacagc aactgtatat
     7801 cccataaata acctctttcg gaattttcta aaaatttcta taatagaagt attatactac
     7861 tttttccatg aattgcaaag ctttttcatc attttttgga aaataaaaat ccccaatcgc
     7921 tagactaggg attaagaggc atcttcatgt gatgagcagg ttcacacaac tcatcaaggt
     7981 ccgctcctgc gttatgacct ccttatgctc aatagtagtc cgaagactac ctatcgactc
     8041 atcgcatatt ctatattact agagatactt gattttgtca ataagaaatt ttttcgatcg
     8101 agtataacaa aaaactctcg tattcaagag tttccctata catatcatgc taattgccgg
     8161 gattgaaccg gcgacctcat ccttaccatg gatgcgctct gccaactgag ctaaatcagc
     8221 ttacttaaga agtatactat attcagaaaa acttgtcaag tttccttaga aattttccat
     8281 aggaaaaagc caggtaagaa ctacctagct tatcttcttc tatttactta aatcaattcg
     8341 acgaccaatt ttaccaataa taatcgctcc aataatcaat gaagcaaatt caccgattcc
     8401 tgttgtaaac caagtaagga agaatggtaa ttctgctaca atatttaact ctgcagcgat
     8461 agtaaacatg gaaattgaaa agaggattga aaagaagaaa tgatcttttc gaatcaatcc
     8521 gttaaagagg taatccttgc tatattttgc aaaaagccaa acacctaaac tgaggaatac
     8581 taaggttgaa ccaccaccaa caaagacatc tagcagtccg aagctaaaga aattagcaat
     8641 catacaaccg atggtaactc cgatgatgta cttaggattg taaaaagcca taaagttcat
     8701 catttctgaa atacgaaact gataagcacc atagctgatg gcatttaggg gcggtgtgac
     8761 agtcaaaacg acgtagatag cagcgacgat tgcaatatct gcaacatcac gaatagttaa
     8821 ttttttcatc ttttctcctt tggcggttat ccgcgtgtaa tatgcttggt gaaagaagct
     8881 aagcaccaag ggttgattca ttcaacctta ctagtatagc acaatagcct gctttatgct
     8941 atacttaaag catgaaaaaa cctattcaaa aattttttaa caatgaaatc ttagcctatc
     9001 tcttttttgg ccttgcgaca actctagttt cgattctctc acgactagtc atttatcaac
     9061 taagtcatca ggaactcctt gctacggcct tggcaaatat tatcggtatc ttatttgcct
     9121 ttatcacaaa tgatacgatt gtatttaagc aaaaaaagaa aaatagactt atccgtttag
     9181 tgaaattttc tcttgctcgc ctttctactt ttctgttaga cttgttttta acttttctct
     9241 ttgtaaccca atttcctcac attatagggc aattcgtaac tgaaaacatt gataagataa
     9301 atgctattga aacaataatt gcacaaatat tcattattat catcaattat atcttgagca
     9361 aggtctatat ttttagaaaa taatgctcca tttatgagcc ttttaattgc cttctataat
     9421 gatttaggat acaatgaaac acgaaacatt gaaaggaaaa cagtatgtta aaaaatctaa
     9481 aatcgttctt gcttcgagga aatgttattg accttgctgt cggtgttgtc attgcctctg
     9541 cttttggtgc tatcgttact tcacttgtaa acgacattat cactcctctt attttaaatc
     9601 ccgctttgaa aactgctaaa gtcgaacgta tcgctcaact ttcttggcat ggagtcggct
     9661 atggtaactt tttaagtgct attatcaatt ttatctttgt gggtaccgcc ctcttcttta
     9721 ttatcaaggg cattgaaaaa gcacagaaac tgactggcat aaaggaagaa aaaactgccg
     9781 aaaaaaaacc aaccgaattg gaagtccttc aagaaataaa agctctcctt gagaaaaaat
     9841 aatcgattaa aaggatctag cgcaaagcta aatccttttt tctttactct ttgggtaact
     9901 tgcctgcttt ttcaagcatt ttcttgatca gataaggcat cttaacattt tcacgacctt
     9961 ttttctcaat gagttttttg acaaattctg gcatctgaag gtcgtctgtc tctttcatga
    10021 tgtccttggt agttgccgaa ggcgctaact catcaaaggt ttcttcttct gggagaaaag
    10081 ttttaaattc tttgtcttcg ttggctctta cagtagcaac taaagcgtca attaaacggc
    10141 tatccgtatt gggcatcggt ggacgatggt aagtgacacc taattcttga cacaaatcat
    10201 aacattccac atcattatcg aacaagactt cgatgtgctc actgataaag ctaatcggaa
    10261 caaaaatata atgttctgga tgttgctttt gttctcgtaa atattctaag acatctggct
    10321 tgatccaagg gattccaata tcgctttcgc tctgccaagt attggtatac tggtctgtca
    10381 ttaggcctag ttgttcagca atgagcctgc tattatcgaa aatctgatcg atataaggat
    10441 ctccatagtc caaggcaaaa atgggaacgc tatgggctga aaagattacc ttgaaggatt
    10501 cttctcccac ctcatttcgt aaaatttttc taatctcatc tgtccagaag tccagcaaag
    10561 actgttgttg ataccagtcc ttaataacta aaaatcggat ctgctggctt tccagaaact
    10621 tttcataccc catgactgag taaaaagaat aatgaggttc caaaatcaag cagatacatt
    10681 cttcaacacc atcagtttcc atctgcttga tcacgtctgg aataaaggga cgagaaaatt
    10741 tgttggcaaa gtagataccg tactcctccc ctaaacgttc tttgactaaa ttgacctcct
    10801 cacgtgtaat ccgctgcaaa ggcgtcccac cgatacgaat atagttgtca tagagagtct
    10861 ggatttcgtg gtcttggggt ctaactccac gacgaatgtt tgtaaagaat tctgctactc
    10921 cttcaaaact aatctcctct ggagatccaa aggtcatcat taatatggct tttttcatgt
    10981 tatcatcctt atgatattat tttctctatc cattctatca tgaaagaaaa aaatagtaaa
    11041 cgtttcctac gtctactatt tcttttgctc ttttgttttt ttatctcgtc ttataattag
    11101 gatgataagg acaagaagtg ggaattccgt gatcgaaata ccgtgttcct actgcttccc
    11161 cactttcatc ctgtgctact ttcaccggtg catccgttat tttagctaaa aggatatgtc
    11221 gtccattagt tatgttaagg aaacaggtgc ttaataaaac aatcttgtcc gttgaggtca
    11281 aggaaacatc tcgcttgtac ttggctttac ttagcagata ttgatagtaa gcctggtgtt
    11341 cctcatcctt ggaactcaag gttcgataaa tctccgtgtc ataggcatcc acttctaaaa
    11401 tcccaaaaat ttcaagacca cgttctcgac cattgtagta gatagaaccg tagcgatgct
    11461 ggtcgaagaa ttccttatca gcaaatttct taatctcacc gaacatagcc cctgaagcca
    11521 tagagtgtcc gtagatgatc gtattaaaat cagtaaagtt gggattaaag cgatagtcga
    11581 gaaaaattga tcccgttatc gtataatcgc caaaggcatt gcggtcaagg tatttgacat
    11641 tatcctgact ttgtagaagc ggatagtcta ccttggtccc atcaaggcta atccaaccgc
    11701 aaacatcttt gttcaagtgc tgtagggttt caaaacctgt gctcgaagca tccttttcaa
    11761 taggattata gtgatcatag tcacttgctt ctactactct ctgccaacta aaaagagcta
    11821 caaaaacaca gaagaatacc agaaatacgt ttttaaatcg cgctcttttg aaccgtcatc
    11881 agcactcctt tctatgcgag atttcttaaa aggaaaggag gcgggacaaa accgcctctt
    11941 ttctattcac ccttagtcag taaaagggtc tgcttcttgc atttttttta ctctctcttc
    12001 tacgacgtag gatcaagtag aggacaagga gtaagaaaat caagagcaag aacaatacaa
    12061 acaagataag gtaccaaatc catagtggga ttgatgagag gaaacgtcca agagacgagt
    12121 catcaaagac actgtatgga aatggttttg atttttttgt atggaaagta tttttaggga
    12181 cagtgtctgt aatctttgcg actacgatat gacgaccatt ggtcacatcg agaaaacagg
    12241 tactgagtag gatgatacga tcactcggtg agagtgagat atcccgcttg tgcatggcga
    12301 ctgagagcag gtggtctaga taggcctgct ggcggtcctc accctgtatt cctggatcat
    12361 agatgttaaa gtcataggca tcaacctcca acatctcaaa gatctcgagc cctttttcca
    12421 caccattgta gtatatacta ccgtaacgat gctggtcaaa aaattcctga tcagcaaact
    12481 tagccacatc accaaacatg accccatttt ctacgtggtg cccgtagata atggtattaa
    12541 agtcttcgaa cttaggatta tttcgtgcat cgagaaagat agcgcctgtc gctgcaaact
    12601 cacctttgga gtccttgttg agatactttt cattgtcctt ggcttggact aagggatagt
    12661 cgatattggt gccgtagaca ttgatccaac cgaggacttc gggattgagt tcttggagtt
    12721 tgctgaagcc tgaaaaactg gccagctcat cctgttgcgt cgtgacaggt cgataagctt
    12781 cgtactcact tgacgaagct aaggagtaga cttggttgga atcccaaagg gcgtagaaac
    12841 caaagagaaa gatcaaagct gcaaaaatga gcagcaagtg attgatcacc ccatccacag
    12901 caagaacgag acgcctagac agactctttt ttggtttctc acgcttggtt ttcatcatct
    12961 gcccccagcc gtttaggctt ctctacgttt tttagacaag acaacaaatc cagccaaacc
    13021 aagaccaatc atcaaaatga atggaaggtt atcaatcaag agaccagtag gagtaacgtc
    13081 gtcgattttg ttagtaactt tgttgtcgtt tttcttttca cctatgagga tatctgttaa
    13141 tgtaaaatct acattttttt gtccttgaga tgtttgttct gcaccatttg tagtataaat
    13201 tgatgaatct gtataacctg ctactccagt ttcagttact gtatatttag aacctgctgg
    13261 tagttgttca actgtaaatg tttcaccatc tttaagagta aaattgtact caactgtatc
    13321 tgctggggtc actactgcca acacctctga cttagatcca tgttttacta taatattcgt
    13381 aacaggggtt tctgctgttt tatttgtact tggaagttga atcttgatat ggaattggaa
    13441 atctcttgtt ttatcccctg aagctccatc aactttttta gtaattgtta atgctttcgc
    13501 attggggtct acttgactcg gatcatcagg attaggctcc ttacttgcgt ctttggtata
    13561 tgtgttatca aataaattat aaacgccttg ttcacttggt tctactttag gggcgttaga
    13621 tttattattt tccttaaagt atactgatga aatatagaca ccaccattta ctttattctt
    13681 aacaataacg tgcatttcat atgtacgttt gtcgtatctc atggcatcaa ttgttttacc
    13741 atttttggta atcgcctgcc atccagtttt tgcttccgct acatcataaa cgtattcacc
    13801 agcatggcca taagcaactc ccgaaaaaat gtcttctgtt gctttttgaa tcttatcagc
    13861 agttgggtga tcatttttat tgtattctac atttctatct ggaattgcaa ctccggtttg
    13921 atatggcgca ccgttagaag attgtcctgt tttttctgta aacttaaatg taaatgtcgc
    13981 tgttggtgtt gttattccct ctgcaatatt caacgtttta ttaatcttca caacagcatt
    14041 attttcacca tttgctttgg ttattgctga attatcatcc gcaaaagtcg ggacacctct
    14101 gaatacggac aaagcggtta tagctgcaat gcttgcagtg aatagctttt taaagaatgt
    14161 ttttttcatg attatttctt ttgttttttt attttctttt ttcattatga tcatcaaaag
    14221 cctcgtcttc taattactgt tacaactttt ccttgagtat cctgtatagg gatggttcca
    14281 aaagcacgac tgtctacagc cgttgttcga ttgtccccga ggacaaaaag ttgtcctgct
    14341 gggactttca tcgagaaggt tgccccttcc ttatagagca gcgtttcttt gtagatatct
    14401 tgctcttgtt gaggagaacc gttgatgatg agaccatttt cgtttatatc gattgtactt
    14461 ccttcggttg ctatgacacg cgccactctt tccttacctt tataactata gactagcaaa
    14521 tcaccaatcg aatagcgttt atccaaccta taatagacga ccaagtcgcc atcttttaag
    14581 gcgggcttca tgccatcgtc attgtatctt aataatccaa agacgaaaaa gtaaagggtg
    14641 gctagtacaa atccaaccat cagaagttta ctgactaagt aaaggatatc tccccagaca
    14701 ctcggcggag acttatcccg attgacttgt gttactggtt tcttatgttt ctttttaagc
    14761 agcatcactt cctcctattc accacaaggt gaccaagtaa gatgataaag aagatccctc
    14821 ctagaggaag aacgactcca agcacgagag tgctactcaa gaagtcaatt cccgtttcag
    14881 gtacgctgtt tttattattc gtgactgtcg cagacttatt tgcatcaaga gttccttctt
    14941 ttttatcata ggacacctga tacccacgag acgaacttgc ttcttcttcg atactataac
    15001 gagcatttag aggaaggtcg agaatcttga tggttttatc tttctttaga tcaactgtcg
    15061 ccttaccatt ggtgaattgt agcgt
//
LOCUS       DAVAAL010000038        14925 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVAAL010000038 DAVAAL010000000
VERSION     DAVAAL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.039"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..1126)
                     /locus_tag="VQM60_000993"
     CDS             complement(<1..1126)
                     /locus_tag="VQM60_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET1080873.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMIAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQSPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPKDKDFFARTSPHLLPFFEKNVTLSD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNG"
     gene            1198..1575
                     /locus_tag="VQM60_000994"
     CDS             1198..1575
                     /locus_tag="VQM60_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET1080874.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKNSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            1666..1998
                     /locus_tag="VQM60_000995"
     CDS             1666..1998
                     /locus_tag="VQM60_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET1080875.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            2010..3581
                     /gene="ffh"
                     /locus_tag="VQM60_000996"
     CDS             2010..3581
                     /gene="ffh"
                     /locus_tag="VQM60_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET1080876.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPNRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPNLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(3776..5059)
                     /locus_tag="VQM60_000997"
     CDS             complement(3776..5059)
                     /locus_tag="VQM60_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET1080877.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(5313..6026)
                     /gene="rsmG"
                     /locus_tag="VQM60_000998"
     CDS             complement(5313..6026)
                     /gene="rsmG"
                     /locus_tag="VQM60_000998"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET1080878.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            6120..6680
                     /locus_tag="VQM60_000999"
     CDS             6120..6680
                     /locus_tag="VQM60_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET1080879.1"
                     /translation="MTWIILGVLALVVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            6682..7581
                     /gene="htpX"
                     /locus_tag="VQM60_001000"
     CDS             6682..7581
                     /gene="htpX"
                     /locus_tag="VQM60_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET1080880.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRIFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(7625..9151)
                     /locus_tag="VQM60_001001"
     CDS             complement(7625..9151)
                     /locus_tag="VQM60_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET1080881.1"
                     /translation="MIKINHLTITQNKDLRDLVSDLSMTIQDGEKVAIIGEEGNGKST
                     LLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFFLDSIDLDYSIL
                     YRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLDEPSNDLDLETV
                     DWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLVEHLDYESYSEQ
                     RKAKFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGRLLAKKMKTVLS
                     QEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLSIDDRVLVQKLQ
                     LTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHKKLQLDLSPIAY
                     LSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDLVLRKPNFLLLD
                     EPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSSIYRLTEHGLELVNLEDV
                     "
     gene            9910..10452
                     /locus_tag="VQM60_001002"
     CDS             9910..10452
                     /locus_tag="VQM60_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET1080882.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            10452..11081
                     /gene="nth"
                     /locus_tag="VQM60_001003"
     CDS             10452..11081
                     /gene="nth"
                     /locus_tag="VQM60_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET1080883.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            11292..11813
                     /gene="pyrR"
                     /locus_tag="VQM60_001004"
     CDS             11292..11813
                     /gene="pyrR"
                     /locus_tag="VQM60_001004"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET1080884.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            11832..12755
                     /locus_tag="VQM60_001005"
     CDS             11832..12755
                     /locus_tag="VQM60_001005"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET1080885.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            12805..13884
                     /gene="carA"
                     /locus_tag="VQM60_001006"
     CDS             12805..13884
                     /gene="carA"
                     /locus_tag="VQM60_001006"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET1080886.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            14197..>14925
                     /locus_tag="VQM60_001007"
     CDS             14197..>14925
                     /locus_tag="VQM60_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET1080887.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIH"
BASE COUNT         4392 a         2822 c         3358 g         4353 t
ORIGIN      
        1 ctccattctt ttgtaaaatc tcaatctgtg tccgtggatt ttcagattcg ggacgataga
       61 tatcataagg gaggtcaaag ttcttatgaa tggcagtata gtagtcggga tcaaagccaa
      121 tgtcctcaac caattttctc atgctagcga gttggtcttg atcttcttgt gaaaaggtaa
      181 tggatttaaa gaccttgcgg ttgacaaagc gttgcgataa atctgcgaga attttgtcag
      241 gactggtcat ccagagctgg aagtaggtat tcatcacacc atcatctaga gccagatagt
      301 cagacaaggt cacatttttt tcaaagaaag gcaggaggtg tggggaagtt cgtgcaaaga
      361 agtctttgtc cttagggtag agttccttag cgcgcttgag aagattctgt aggagaactt
      421 ccatagcgcg tgttgctggg tggaaataga cctgcatata catctggtag cgactgagga
      481 catagtcttc gatggcgtgc atgccattgc gctgaaaggc gataccattt tcgataggac
      541 gaatgactcg gaggattcga gtcaggtcaa attccccata ggatgctcct gtaaaatagg
      601 agtcacgcaa gagatagtcc atgcgatctg cgtcaatctg actagaaatg agttgtacga
      661 cctgcttgtt aggataggta tggtctatga cactggcaac tttctctgga aaatcgggcg
      721 ctacttgtag caagacttgg tgaatctctg tctcaggact ttgaataatc tcctgagtaa
      781 tagcttcatg gtctgtatca aagagatgtt caaaagtatg ggagtaggcg ccatgcccaa
      841 ggtcatgtag gagagcagcg atcatggtca agagagactc ggcaggattc cattcctcag
      901 gatatttttc ttcgaaaatc tctgtgatgc gtcgtgcaat ttcatagact cctagacagt
      961 gagagaagcg actgtgttct ccaccgtgga aggtataact ggaagttccc agttgtttga
     1021 tccggcgcaa acgctgaaat tcttttgtat taatcaagtc atagatgatt tgattattga
     1081 catggatgta gttgtgaact gggtcacgga atactttttc gttcatatga ccattatagc
     1141 aaaatcctgc tctttttggt aaaatagtag gacaagagac aagaaagagg gcctgatgtg
     1201 aagattcgga tgcgaaatac aattcagttt gatgagcagt tggaagtgat tgaccagctt
     1261 tatgatgtgg aagtgcatga aaaaggagat tatagctacc tgcttttcta taatgaggaa
     1321 aaggaaaaag tagtgattaa atttcatggt caagaacttg tgatgagccg attttctaat
     1381 cccaagacca ttatgcgctt tctaaagaat agcgatagtt tagcctatat tcctacacct
     1441 atgggcatgc aggagtttat catccaaacg agccattatc aagttgatag gcaaaagatt
     1501 gaactagatt atcaactaca aaatcaagag ggacatcctt ttgccagcta tcaattggaa
     1561 attacttggg gctaggctca tgtctttttc aaatttagct atctatcttc ttggatcggc
     1621 ttcctagtgt ggtagattag gctaattttt ggtataataa aagttatgga aatcgaaaaa
     1681 accaatcgta tgaatgcgct ctttgaattt tatgcggcgc ttttgacaga taagcaaatg
     1741 aattatatcg agctctacta cgctgatgat tacagccttg ctgaaattgc cgaggagttc
     1801 ggtgtcagtc gtcaggctgt ctatgacaat atcaagcgaa cagaaaagat tctggaagat
     1861 tatgagatga aattgcacat gtactcggac tatattgtcc gcagtcagat ttttgatcag
     1921 attttggagc gctatcccaa ggataacttt ctgcaggagc agatagaaat tttaacaagc
     1981 attgataata gagaataaga ggaagaaaaa tggcatttga aagtttaaca gaacgtttgc
     2041 agaacgtctt taaaaatcta cgtaaaaaag gaaaaatctc tgaatctgat gtccaagagg
     2101 caaccaaaga aattcgcttg gccttgctcg aggccgacgt tgccttgcct gttgtaaagg
     2161 actttatcaa gaaagttcgt gagcgtgcag tcgggcatga ggtcattgat acacttaatc
     2221 ctgcgcaaca gattattaaa atcgttgatg aggaactgac agccgtttta ggttctgata
     2281 cggcagaaat tatcaagtca cctaagattc caaccatcat catgatggtt ggtttacaag
     2341 gggctggtaa aacaaccttt gctggtaaat tggccaacaa actcaagaaa gaagaaaatg
     2401 ctcgtccttt gatgattgcg gcggatattt atcgtccagc tgccattgac cagcttaaga
     2461 ccttgggaca acagattgat gtgcctgtct ttgcacttgg aacagaagta ccagctgttg
     2521 agattgtacg tcaaggtttg gagcaagccc aaactaatca taacgactat gtcttgattg
     2581 atactgcggg tcgtttgcag attgatgagc tcctcatgaa tgagcttcgt gatgtgaaag
     2641 cattggctca accaaatgaa atcttgcttg tcgttgatgc tatgattggt caggaagcag
     2701 ccaatgttgc gcgtgagttt aatgctcagt tggaagtgac tggggtcatc cttaccaaga
     2761 ttgatggcga tactcgtggt ggtgcggctc tatcggttcg tcacatcact ggaaaaccaa
     2821 tcaagttcac tggtacaggt gaaaaaatta cagatatcga aaccttccac ccagaccgta
     2881 tgtctagccg tatccttggc atgggggata tgctcacttt gattgagaaa gcttctcagg
     2941 aatacgatga acaaaaagcc cttgaaatgg ctgagaagat gcgcgaaaac acctttgatt
     3001 ttaatgattt catcgatcaa ttagatcagg tgcaaaatat ggggccgatg gaagacttgc
     3061 tcaagatgat tccaggtatg gccaacaatc cagcacttca aaacatgaag gtggatgaac
     3121 gccagattgc tcgtaaacgt gccattgtgt cttcgatgac acctgaagaa cgtgaaaacc
     3181 cagatttgtt aaatccaaac cgtcgccgtc gtattgctgc tggttctgga aatacattcg
     3241 tcgaagtcaa taaattcatc aaggacttta accaggctaa acagctcatg cagggtgtta
     3301 tgtctgggga tatgaataaa atgatgaagc aaatggggat taatccaaat aaccttccta
     3361 aaaatatgcc aaatatggga ggaatggata tgtctgccct tgaaggaatg atgggacaag
     3421 gcggtatgcc taacttatca gctctcggag gagcaggaat gccagatatg agccagatgt
     3481 ttggtggcgg tttgaaaggt aaaattggtg aatttgccat gaaacagtcc atgaaacgta
     3541 tggctaacaa aatgaagaaa gcgaagaaga aacgcaagta agacaagaga ttgatgcaaa
     3601 tctcatttga tgagaaaata aagtaaaaat ttctatgata aaacgcacag tatcaaggtt
     3661 ttagaatatc tgatattata cgttttttga ttttaataat tttgttaaga cacttttatt
     3721 atggtatacg aaaaatccac tcagctgtct gagtggatta ggtgtattta gactcttagt
     3781 ctttattttc gtatggcaag atcaagttca agatgattcc tgtcatggct gaaagggcag
     3841 tacctgaaag tgtaactgga ccaagtttaa ggatagctcc tccaagtcca agaaccaaca
     3901 tagcacttgc gatgatgagg tttcgcattt gagcgaaatc aacacgttct ttaatcaaga
     3961 ctttcaaacc attgctggcg ataaccccat agagaaggat tgacatacca ccaagtacag
     4021 cgtttggaat agttgaaatc aaggcagtga atttaccaag gaagctgagg gcaatcgcga
     4081 tgaaggcagc gttacggata actgagacag aagcgatacg agtcataccg ataacccctg
     4141 tattttctcc gtaagttgta ttggctggtc caccaaggaa ggcagaaaca gaagttgcga
     4201 taccgtcacc aataagagta cggtgaagac ctggttcttt taagaattga cggccacaga
     4261 tttgacccaa aacagtatgg tctccgatat gttcagaaat tgttacgata gcgattggca
     4321 agatagcgat ggcttctgga ccaaagtaaa gattgtactc tttaaaggca ccacctgtgc
     4381 taaatggcaa gtagaaacca ggaatttcaa accagttagc ttctaggaca ggcgtaaagt
     4441 caaccaaacc aagagttagt gcgaagaggt aaccaccgat aatggcaaag aggaatggaa
     4501 tgattcgtag gaagcctttt ccttttgtat tgataaaggc agcaattagg aaagtaacaa
     4561 cggctaccag agcatttttc caatttccgt ctgctacaag acctgcattg gtaacagctg
     4621 aacctgcaag tccaagaccg ataacgatga tcataggacc gataatgatt ggtggcaaga
     4681 gtttatcaat ccattttgtt cctacaaatc ggatgctggt agcaacaagg acatagacca
     4741 aaccagtcaa gataacccct gtttgggcag cagatacatc cccccccatt tctttcatag
     4801 ccagtgacat agctgtgata aaggcgaatg aagaacctag ataaactgga actttaaaac
     4861 cagtagcaat catgtagatg agtgttccaa cacctgaagc aaaaagggca acagatacag
     4921 gcattcccaa aatcaatggt accaagatgg tcgcaccaaa catggcgaaa acgtgttgga
     4981 aactaaggag aattcctttt ccagccgaag gacgttggtc aacgtctagt aacaaatcaa
     5041 cagttgattc ttgtttcata taaacctcac ttttgggcat caaaaaagag cccattattt
     5101 tacagcaata ggccctcaga gtaagacttc tttaaaattt agattttaaa gagttttaaa
     5161 tatttctgcg tcttcgtcac ctcacgggat gacattaaaa acctttgctt aggatagtat
     5221 agcagaaaaa aatgattttg taaatcattt tttcccgagc ctagaaataa aagagcgagg
     5281 ctgattttgt aaacctttgt ttactaaaaa atttaaagtg ggcgtttatt tggcatacca
     5341 gccttacgtg gatatttatt tggtgtttct tttttctttt ctaccactgt gatatagcgc
     5401 ggatctctat tcggtagggc gtagctgaga ttgtcttcga ccttactaaa aaggagattg
     5461 agggcattct tagcttctaa taattcctca ggcgcattgc tagccttgag tgctaatagt
     5521 ttgccaccaa ccttaaggta gggaatagtc aattcagata ggacctgcat acgggcaacc
     5581 gcacgagctg ttacaaaatc atattgagca cggaagttct tgtcttgggc aaaatcttcg
     5641 gcacgtccgt ggtagaaatg aactccgttc aaatccagtt cttgagccaa gagttgtagg
     5701 aagttgatgc gcttattgag tgaatcaata atggtcacat ctaactccgg atagagaatt
     5761 ttcattggta gactcggaaa tcctgcccca gccccgatat caagaagttt gatagtttca
     5821 ttgggaatca aaccttgaag aatgggtgca atcgaatcgt aaaaatgttt gagataaact
     5881 tcttccttgt ccgtaatcgc cgtcaaatta atcttctcat tccactcgac caagagctca
     5941 aaataacgtt caaattgttc tttttgctgg tccgaaagtg gaagattctg ctcggcaagc
     6001 aagttgtaaa atgtttttgg tttcatagtt atttccttct ttagtatcat cttattttac
     6061 cacaatcata gaaaatttga tatactggta ctaatctaaa agcagaaagg acttatatta
     6121 tgacttggat tattcttgga gttttagctc ttgttgttat ttttgtgatt gttagctata
     6181 acggtttggt taaaaatcgt atgcaaacca aggaggcttg gagtcagatt gatgttcagt
     6241 tgaaacgtcg caatgatctc ttgccaaact tgattgagac tgtaaaaggt tatgccaaat
     6301 atgaaggttc tacccttgaa aaggtggcag aactacgtaa ccaagtggcg gcagcgactt
     6361 caccagcaga agctatgaaa gccagtgatg ccctcactcg tcaggtttca ggtatttttg
     6421 cagttgcaga aagctatcca gatttgaaag ctagtgctaa ctttgttaaa ttgcaagagg
     6481 agttgacaaa cacagaaaat aaaatttctt actctcgtca actctataac agtgttgtca
     6541 gcaactacaa tgtaaaatta gaaactttcc cgagcaatat tatcgctgga atgtttggat
     6601 ttaaagcggc agatttcctt caaacacctg aagaggaaaa gtcggttcct aaagttgatt
     6661 ttagcggttt aggtgactaa gatgttgttt gatcaaattg caagcaataa acgaaaaacc
     6721 tggattttgt tgctggtatt tttcctactc ttagctcttg ttggttatgc ggttggttat
     6781 ctctttataa gatctggact tggtggtttg gttatcgcac tgattatcgg ctttatctac
     6841 gctttgtcta tgatttttca atcgacagag attgtcatgt ccatgaatgg agcgcgtgag
     6901 gtggatgagc aaacggcacc agacctctac catgtagtgg aagatatggc tctggtcgct
     6961 cagattccta tgccccgtat tttcattatt gatgatccag ccttaaatgc ctttgcgaca
     7021 ggttctaatc ctcaaaatgc ggctgttgct gcgacttcag gtctactagc tatcatgaat
     7081 cgtgaagaac tagaagctgt tatgggacat gaagtcagtc atattcgtaa ttatgatatc
     7141 cgtatttcga ctattgcagt tgcccttgct agtgctatca ccatgctttc tagtatggca
     7201 ggtcgtatga tgtggtgggg tggagcaggt cgcagacgaa gtgatgatga ccgagatgga
     7261 aatggtcttg aaatcattat gctagtggtt tccctactag ctattgtact ggcacctctc
     7321 gctgcaacct tggttcagct cgctatttct cgtcagaggg aatttctggc agatgcatct
     7381 agtgtcgagc tgactcgcaa tccccaggga atgattaatg ccctagataa gttggacaat
     7441 agcaaaccta tgagtcgcca cgtcgatgat gctagcagtg ctctttatat caatgatccc
     7501 aagaaaggtg gggggttcca aaaactcttt tatacccacc cacctatctc agaacggatt
     7561 gaacgtttaa aacagatgta aaagaatcca gaggtcgtct ctggattttt gctatgttgc
     7621 aaatttatac atcttctaaa ttaactaact ccaaaccgtg ttctgtcaag cggtagatgc
     7681 ttgaacagac ctcttttaag aaacgacggt catgcgaaac agtgatgaga ccgcctggat
     7741 aggttgcaaa gagttttctg atttggggtt gagaagtggg agaaaagttt cgtgtgggtt
     7801 catccagcag gagaaagttt ggtttgcgca ggactaaatc caaaagcagg agttttccct
     7861 gttgtccgcc agataaggag cgaatttgat gctgcatttc tggataactg aaattgagac
     7921 tagctaggtg agattggatt ttctgtagtt cctctttttc cccagttttg ctgaggtagg
     7981 ctattggtga taaatccaat tgcagttttt tgtggtaatc ttgtggcata aaaccaaggg
     8041 aaatctctct tttatcattc agaagttgct ttaatttggc taacagagtt gatttcccaa
     8101 caccatttgg cccgataata ccgatttttt cttgcccacg gacagttagt tgtagttttt
     8161 gaaccaaaac tcggtcgtca atggacaaat tttctttttc cagttggact aagactttag
     8221 aagctggtaa tggttggatg tctgaaaaga aaagttggat ttgttcctct tcaagtggct
     8281 tttgagtcat ggactgagct gccttttcgt agcgtttttc ttgtgagagg acagttttca
     8341 tctttttagc caataggcga ccggcagtac tgtctttggt agctcgaagc gcagtttcta
     8401 cattttgttt gactctccga tgtttttcca tggttttatc gtaggctctt tggttgttag
     8461 cagcttgctg actttgtttg gcaaatttag cctttctctg ctcactatag ctttcatagt
     8521 ctaaatgctc tactagcgtt tccgcttctt tacggtgttt gaccagtcgc aagtgaacaa
     8581 tagtgtctgc cgtttcagaa agaaagtctt catcatggga aatgaaaata acggtttgcc
     8641 tggtcttttg aatctggcct tttaaccaat caactgtctc aaggtctagg tcatttgaag
     8701 gttcatctaa aaatagaatc tcaaagggtt tggctaactc atggataagc tgaattttca
     8761 aagcttctcc ccctgataga ttgccaatct cttggtcact tgcgaaacga ttgctatcaa
     8821 aatgcaattc ctccgccaaa cgatagagga tactgtagtc taaatcaata gaatctaaaa
     8881 agaagtagtc gtgtaaagtt ttctttttca gctcctcggg aagtttttga ggaatgtagg
     8941 ccagtgactg ataatcagac tggatgtctc ccttgatagt gaaatcagac aaatcttccc
     9001 ccattaaagt tttaagtaag gttgatttac catttccttc ttcgccaata atagctacct
     9061 tttccccgtc ttgtatggtc atgcttaggt cagagacaag gtctcgtaaa tctttgtttt
     9121 gtgtgatggt caggtgattg atttttatca taactattgc cctttctaga atgttcatag
     9181 tgcataacaa aaagctctac tttacctagt agagcccaaa gtctatgtca aaaaaactct
     9241 attaacatgc cagaaaaagg gcataaaaga gcacacaaaa agagacaaaa acaagatcta
     9301 ctaaataaag gttctttatt tgatagactt agttgttcat gtctccctta cctcctagta
     9361 ttagtatctt tatactatac ttttaaggaa attttgtcaa cagaaaactg gaaattctat
     9421 gctctaaaat ccaaaaacag gtctatagag acttagtacg tgtttgacat gctcgtaatg
     9481 gattttatca tagtttcgcc aagcagtgcg ttcttgattg tttagtttta ggctacccag
     9541 tccaatcata ggactggtgg gttggtaaaa tttctcaaga tcaattaaga tactgttgtt
     9601 caacttttcc gttttttgag ttcttgcata gtagtgttta gcagctcttg tagtcgaata
     9661 tcatctgccg taccctgttt acagttttgg tagcttttat ttaacttgga taggagttga
     9721 atgagtttct tgtcttccgt gtgagcatcc tccttgatct gatgtactct ttccctatag
     9781 tatagcacta aaaaataaaa ccgtcatagt gaatatgtta aaatttcgaa ttttaattac
     9841 aaagtttggt gtgctgattt gtagcctttt catggtataa tcaagagagt aaatctttat
     9901 ggaggaagta tgaaattaaa tattcaagaa attcgtaagc agtctgaagg tttgaacttt
     9961 gaacaaacgt tagatttagt tgatgacctg cgtgcacgta atcaagaaat tttagatgta
    10021 aaagatatcc ttgcagttgg gaaagtacaa tatgaagacc gtatgtattt cttagattat
    10081 caactatctt ataccattgt tcttgcttcg agtcgcagta tggagccagt tgagttagtt
    10141 gaatcttatc cagtcacgga agttttcatg gaaggcgcaa ctaaccagct agatcaagaa
    10201 gttttagatg atgacttggt cttgcccatc gaaaatgggg agcttgacct tgctgagagt
    10261 gtatcagaca atatcctgct aaacattcct atcaaggtct tgacggctga agaagaagct
    10321 ggtcaaggat ttgtctcagg aaatgactgg caaatcatga cagaggaaga ataccaagct
    10381 caacaagcag taaagaaaga agaaaacagt ccttttgctg gcttacaagg actatttgac
    10441 ggagatgaat aatggtcttg tcaaaaaaac gagcacgaaa ggtgctagaa gaaatcattg
    10501 ccctcttccc agatgccaag cctagtcttg attttaccaa tcattttgaa ctcctggttg
    10561 cggtcatgtt atcagcccag acgacagatg cagcggtaaa taaggccaca ccaggtctct
    10621 ttgttgcctt tccaacaccc caagctatgt ctgtagcgac agagagtgag attgcttcac
    10681 atatttctcg cttgggattg tatcggaata aagctaaatt ccttaaaaaa tgtgcccaac
    10741 agttactaga cgattttgat ggtcaagtcc ctcagacacg tgaagaattg gagagtttgg
    10801 caggtgttgg tcgcaagaca gccaatgttg tcatgagtgt aggatttggg attccagcct
    10861 ttgcagtgga tactcatgtg gagcgtattt gcaaacacca cgatattgtc aaaaaatcag
    10921 cgacgccact tgaggtggaa aagcgggtca tggatatctt gccgcctgag cagtggttag
    10981 ctgcccatca ggccatgatt tattttggaa gagccatttg ccatccaaaa aatccagagt
    11041 gtgaccagta cccacaatta tatgatttta gcaatttgta agatgctaca ttgaaacttt
    11101 ttgcttgatt ttaagcatgc tttgtgctat agtaaatgat aactaaatat tcctttaaac
    11161 ctgtcccgtg aggcaggcaa ggagcgcgat aaagtagaag ggtgaagtct atactgtgtg
    11221 cagtagggct cgcttggcta taaatttcaa gtctccttgt ttaaggagac ttttcttttt
    11281 ggaggtttgt catgaaaaca aaagaagttg tagacgaatt gaccgtcaaa cgagcgatta
    11341 cgcgtattac ttatgagatt atcgaacgca acaaagattt gaataagatt gtcttggctg
    11401 gtattaaaac tcgtggtgtc tttattgccc accgaatcca agaacgtttg aagcagttag
    11461 aaaatctttc agttcctgtt gtggaattgg atactaaacc tttccgtgat gatgttaaaa
    11521 gtggagaaga tacttctttg gtttctgtcg atgtgacaga ccgcgaagtt atcttggtgg
    11581 atgatgtgct ctatacaggt cgtaccatcc gtgctgctat tgataatatt gtcggtcatg
    11641 gtcgtcctgc gcgtgtgagt ttagcagttc tagtcgatcg tggacataga gaattgccaa
    11701 tccgtccaga ttacgttgga aaaaatatcc caaccagtcg ttctgaagaa atcatcgtag
    11761 agatgacaga acttgatgac caagacagag ttctgattac tgaagaagct tagaaagtta
    11821 aaggagtagc catgtcagaa aatcaacaag cattgaacca tgtggtgtcc atggaagacc
    11881 tcactgtcga tcaagtaatg aaattgatca agcgaggaat tgagtttaaa aatggagccc
    11941 agcttcccta tgaagaccat ccgattgttt ccaatctctt ctttgaggat tctacacgga
    12001 cacataagtc ctttgaagtc gcagagatta aacttggatt ggaacgactt gactttgatg
    12061 tgaagactag ttcggttaat aagggtgaga cactttatga caccattttg actctgtctg
    12121 ctttaggagt ggatgtctgt gtgattcgcc atccagaggt cgactactat agagaattga
    12181 ttgctagtcc aacgattacg acttccatca tcaatggtgg agatggttcg ggccaacacc
    12241 ctagccagag cttgcttgat ttgatgacca tttatgagga atttggccac tttgagggtc
    12301 ttaaagttgc tattgcaggt gacttggacc actcacgcgt tgccaaatcc aatatgcaga
    12361 ttttgaaacg cttgggatct gaactctttt tcgctggacc tgaggaatgg agaagtcaag
    12421 agtttgcaga ctatggacag tttgtaacca ttgatgaaat cattgatcag gttgatgtca
    12481 tgatgtttct ccgtgtgcaa cacgaacgcc atgatagtgg agctgtattt tcaaaagaag
    12541 actaccatgc ccaacatggc ttgactcaag aacgttacga tcgtttgaaa gaaacagcaa
    12601 tcctcatgca cccagctcca atcaatcgtg atgtagaaat agcagaccac ttggttgaag
    12661 caccaaaatc acggattgtc caacaaatga ccaatggtgt ctttgttcga atggcaatct
    12721 tagaatccgt actagcgagt agaaacgcca actaaaacac aagacgttaa aagacgccag
    12781 tgagcgtcca cgagaggtga aaatatgaca aaaagaattc tagtattaga agatggcaca
    12841 gtttttgaag gcaaggcctt cggagcagat attgatgtaa caggcgaaat cgtctttaat
    12901 acagggatga ccggctacca agaatccatt acagaccagt cttataatgg acaaatcttg
    12961 acctttactt atcctttggt aggaaattat ggtattaatc gtgatgatta tgaatccatt
    13021 attccaactt gtaagggagt cgttgttttt gaagaagcgc gtagagctag caactggcgc
    13081 aaccaaatga ccttggatga atttttgaaa gccaagaaaa ttccagggat ttcaggaatt
    13141 gatacgcgtg ctcttaccaa gattatccgt aagcatggta ctatgcgtgc aaccttgacc
    13201 catgttgggg acagtatgga ccatgtgacg gaccagctcc aagcaacagt tttaccgaca
    13261 gacaatatca aacaggtttc tactaaaacg tcttatccag ctccaggagt tggtttgagc
    13321 gtggtgctag tggactttgg tctcaagcac tcaatcctac gtgaactttc taaacgcaac
    13381 tgtaacgtga cagttgttcc ttattcgaca acggctgaag aaattctcca tctcaatcct
    13441 gacggagtta tgttgtcaaa tggtccaggt aacccagaag acgttccaca agcactcgac
    13501 atgattcgcg gtgtacaagg aaaaattcca atctttggta tttgtatggg acaccaactc
    13561 tttgcaatgg caaacggggc taagacctat aagatgaagt ttggccaccg tggattcaac
    13621 catgcggtac gtgaaatcgc aacaggacga gtagacttca ccagccagaa ccatggttat
    13681 gcagtcagcc gcgaggattt gccagagcac ttgattatta cccacgaaga aatcaatgac
    13741 aagtcagtgg aaggtgtgcg ccacagatac caacctgctt tctctgttca ataccaccca
    13801 gatgcagctc ctgggccaca cgacgctagc tacctatttg acgagtttat cgagatgatg
    13861 gaagttttta aacaatcaaa ctaagaacga gtaaaagctc gtcggagaat ttatgtaact
    13921 gaacacggac ggaaagctca gaatgtagag aaaccaactt ggattgtcaa tccttccttg
    13981 atttcctaaa attcaatcgc tttctattcg tcctttgtat cttatttaat gtcgccctga
    14041 gaggcgacga atagaaagga gaagggtgct ccgtatttgc tgaactgaat acgggctacg
    14101 gacggtgcta aaaagatagt tattcctaga actgacagtt cttcgtcata actcctattt
    14161 tagctttgtc cgcttgacac ccttaatatc ttatatatgc ctaaacgtac tgatattcaa
    14221 aaaattatgg tgattggttc tggtccgatt attattggtc aggctgctga gtttgactac
    14281 gctgggactc aggcttgctt gtcgttgaaa gaggaaggtt atgaggttgt tttggttaac
    14341 tcaaatcctg caaccatcat gacggacaag gaaattgctg acaaggttta cattgaaccg
    14401 attacacttg agtttgtgac acgtattctt cgtaaggaag gtccagatgc cttgctacca
    14461 acactcggtg gtcagacagg tctcaacatg gccatggaat tatctaaaaa tggtatccta
    14521 gacgagcttg gcgttgaact tctgggtacc aaattatctg ccattgacca agcggaggac
    14581 cgtgacctct ttaaacaatt gatggaagag cttgaacaac ccattccaga atctgaaatt
    14641 gttaacacag tagaagaagc agttgccttt gcagcgacaa ttggctaccc agtcatcgtt
    14701 cgtccagcct ttacccttgg tggtactggt ggtggtatgt gtgccaacga gaaagaattg
    14761 cgtgaaatca ctgaaaatgg tttgaaattg tcacctgtta cccaatgttt gattgagcgt
    14821 tcgattgccg gtttcaagga aattgaatac gaagtgatgc gtgactcagc tgacaatgcc
    14881 ttggttgttt gtaacatgga aaactttgac ccagttggga ttcac
//
LOCUS       DAVAAL010000039        14741 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVAAL010000039 DAVAAL010000000
VERSION     DAVAAL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.040"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(19..168)
                     /locus_tag="VQM60_001008"
     CDS             complement(19..168)
                     /locus_tag="VQM60_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080888.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFMAL"
     gene            complement(161..445)
                     /locus_tag="VQM60_001009"
     CDS             complement(161..445)
                     /locus_tag="VQM60_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080889.1"
                     /translation="MVWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            complement(1042..1212)
                     /locus_tag="VQM60_001010"
     CDS             complement(1042..1212)
                     /locus_tag="VQM60_001010"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET1080890.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEKAG"
     gene            complement(1866..3089)
                     /locus_tag="VQM60_001011"
     CDS             complement(1866..3089)
                     /locus_tag="VQM60_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET1080891.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            3256..4578
                     /locus_tag="VQM60_001012"
     CDS             3256..4578
                     /locus_tag="VQM60_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET1080892.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            4580..6466
                     /locus_tag="VQM60_001013"
     CDS             4580..6466
                     /locus_tag="VQM60_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET1080893.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKAFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            6634..6978
                     /locus_tag="VQM60_001014"
     CDS             6634..6978
                     /locus_tag="VQM60_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET1080894.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            6988..8400
                     /gene="lacG"
                     /locus_tag="VQM60_001015"
     CDS             6988..8400
                     /gene="lacG"
                     /locus_tag="VQM60_001015"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET1080895.1"
                     /translation="MENLQFKVLPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGDANVLAVHYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKLGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            8469..10148
                     /locus_tag="VQM60_001016"
     CDS             8469..10148
                     /locus_tag="VQM60_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET1080896.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDISVMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLVTSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGVESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLMLVVDILIYLPFCRAYDRQLLMKEDVVSSNDIILEEETSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(10274..11713)
                     /locus_tag="VQM60_001017"
     CDS             complement(10274..11713)
                     /locus_tag="VQM60_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET1080897.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(11717..13066)
                     /gene="trkA"
                     /locus_tag="VQM60_001018"
     CDS             complement(11717..13066)
                     /gene="trkA"
                     /locus_tag="VQM60_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET1080898.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            13254..14102
                     /locus_tag="VQM60_001019"
     CDS             13254..14102
                     /locus_tag="VQM60_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET1080899.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            14117..14665
                     /locus_tag="VQM60_001020"
     CDS             14117..14665
                     /locus_tag="VQM60_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET1080900.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
BASE COUNT         4510 a         2319 c         3085 g         4827 t
ORIGIN      
        1 ggttacccac tacaaatatt atagagccat aaaagcatca acatcttgtg tggcacgtaa
       61 accatgatat tctaagacat aaccatcgac acagataatt gttaaagtca gattttcctg
      121 aattaattcc ttatttaggg cttggaagac cggtttcatt ttatccatgt ttgtatttct
      181 ccatcaattt ttgaacattg gttaacttag caggtttatt taatgaaatc acatatttat
      241 gagttggagt atgctcttga aaggcatact tcaagctaat tttttgaaaa cgggttagtt
      301 ctttggagtc tagcaacttt tctacatctt ggtttgttaa ataagaatag tcttcaaaaa
      361 taacttttcg caaatagcga aaccaatggc ttgcgttgaa ttgtgattgt ttggatgcct
      421 gtatcatata atcgtaccaa accattgttt tcctcctata aaatttagtt tatattcaaa
      481 aatgtagttt ttcttaatta gctcgtatct ataggatgat actgaaaatc ttcttgttgc
      541 tttaaatcac aatcgaaaaa ttctgttttt catcttagaa aagaatgttt agaaatgtaa
      601 aatgaacctt ttttcttatc cgaaaacctt actaaagtat ataatttagg aagtcaattc
      661 tgtatgataa tgtttgtact atcgtcgagt ttttgcttga ttgcctaacg agataaattt
      721 tagaagaatt ttcggtgtga tttgacaagc ttctgactgg aaggatagtt acactagtat
      781 tgtttattaa aatgagcaga tagagaaaat ttggaatcta tttgcttatt atcaatcgtt
      841 tttttagctt atttgtctta gattggtata gaactgatct atggattaat cctcttgtat
      901 cttatcctgt tcctaaatat gttggtagaa aaatgaagca gttgttacga gtgtctgtat
      961 tgggaaaaat tttgtctata caattcttta aatgatatga aggctaggaa tgccaaaaca
     1021 ctgacatatt tcgtttaaag cctatccagc cttttcaatt gtatttgtag aaatgctatc
     1081 agtaatatat tcgcgaacac tatgatttac aacagtgtgg tcgtacatct ttgtaacttt
     1141 attatacaac aaccatttac aggtgtaaag ttgagcagtg tctttagtaa atttagggac
     1201 ttgttttgtc atagttttga tacctgtatt cgttgtttca tgagtatttg acgtttcttt
     1261 tcatgtaggc atttcaacaa ttagaatgat atttttccga ggatgaactt atatattgtg
     1321 actttttaga gttatgatgc ctcttgattt gactatcgct atcaatttca tccactttta
     1381 cagtttcatg gttttatgaa ccgaaacatc taggtacagg ttttagcatc aattgattgt
     1441 ttttgagcca tatgaagttg aaacgtggag atgcttttct tgtgataacg ccctattcaa
     1501 agaggcataa ccgtttttgg agagagttca gtactatgtg gcgctaaata ctatatgtaa
     1561 aacatatatt aaacaagatt taactaagga gaattgtaaa aacaaaaatg agaatttcat
     1621 ataaagttta ttctaggagt atgtatcttg aataaaaatt gttatttaag ttctgggggt
     1681 tatgatgaat gtttttataa taataaaatg cagctagaag aaaagttatt ttaatataaa
     1741 tcactcaaca tagcgagaag gcggtttcct atatggaagt cgtcttgttt tcatattgtc
     1801 taaatacgag atatttacta caggttatag ttgatttaaa caggtgtata gatttctaat
     1861 atatattaag gtgaaataat atcttgtaat acattgctat gctctatgaa aaaagcaacg
     1921 atagctaatg cacaagctcc tatacttcca cgatgtttgt tgtaaggtaa aatttcaatt
     1981 tctatattac gagtaaaggg aacgaagtgg agctggtctt ggattttatc cctgacttca
     2041 tgagtgaaag gttgataatt aagcaattga ctgttgatat agattttatc agcaggattg
     2101 atgaggagaa gatttgcaat agaagtggcc aataaattga ctcctttatc aatttgttgg
     2161 cgtaaagcgg agtcgcctaa attataagcc gttaaaaggg tgtctaaatg aatgtctttt
     2221 tcagtcttta caaggctttt tagtacagtt agttgggaat ttttaaataa taattgggcg
     2281 tgtttgatta accaagcatc cgaaatatat gtttgtaaac aacctttttt tccgcattca
     2341 caatattgcc cattcaattc gacaatggtg tgtccaattt ctccgatata aggattttta
     2401 gaggctccta ttttttcttt tgtgaaaaag gaagtgtaaa tacctaatcc agcgtgtagg
     2461 aaaataaagt tatcgggggt attgtgtgga ttaaaaaggt attgtcctat agccatacaa
     2521 tcgatattat tttttacaat aaaaggaaaa ttgaatcttt taattacatt taataaatta
     2581 aaagattccc atatggggtt gtttgtaatg atacttccgg agtctttgtt ataatgtcca
     2641 ggtattgata agccaaatcc aagtaatttt gtttccaaag ggacgtactc tatagctgtt
     2701 tttatcatat tggtaatggt ggaattgatt tctgaaatag gataatcttc attcaaaatt
     2761 tctgtctcga aaagagtttt cccttgtaag tcacatagac tgagaaagat acctaggacg
     2821 tttatttcaa tacctccaac aacatacgga atattatctg tgatagaaat agtttttcta
     2881 cgtcttccag atccggaaaa ctcgctaaat tcatcaccag tttcgtatat gagggactgt
     2941 tcgcttagct cttttatgat gttagtcgtt gttgctggag taagtcctgt ttcattaaca
     3001 atatctgtac gggatgttgc tggatttcga tatagaaact ccagtatagt ggctctattt
     3061 tgtaaaaagg ctgattgttt tgtaatcatt tgtttcctcc tttggtttat tttatattaa
     3121 acataagaaa aaatcaatta aaattattat tctatacaaa gaaaaaatgt tgtaaaaaca
     3181 acaaaaaata tattgacaac gctttctgtt agtggtatat ttaatttata aaaaataaaa
     3241 aaaggagttc cggtgatgaa caatatttta gcgtttttag aaacaaaagt cgctccgttt
     3301 ggtgaaaaag ttggcaacca acggcatttg aaagctattc gtgaaggatt tatgatggca
     3361 atgcctttga ttttagtcgg ctctttattt cttattctaa tcagttggcc tcaagaggat
     3421 tttacaaatt ggctgaatag tgttggattg ctaagtatct tgacaactat gaatcagtca
     3481 acagtagcga ttatctcctt ggtcgcttgt ttcggtattg cctacaggtt gtcggaagga
     3541 tatggtacag atggtccgtc ggcagggatc atagccttat ccagttttgt attgatggca
     3601 cctcgttttt cgagtatggt ttatgataaa aatggggagc aggtcaagca gttatttggc
     3661 ggcgcaatac cattttctag cctgaatgca tcttctttgt ttatggcgat tactattgga
     3721 ttggttacag cagagattta tcgtatgttt atccagcgcg gaattacgat aaaaatgcca
     3781 agtggtgtcc cagatgtggt aagtaaatca ttttcagctc ttttacctgg ctttactact
     3841 tttgttttgt gggctctggt cttaaaaggt cttgaagcgg caggagttgc aggaggtctc
     3901 aacggactcc taggtgcaat tgttggaaca ccgcttaagt taattgcagg aacgcttcca
     3961 ggtatgattc tatgtgttat tgtaaactca ttcttttggt tctgtggagt taatggggga
     4021 caagttttaa atgcttttgt agacccagtt tggttacaat ttactacaga aaaccaagaa
     4081 gctgtggctg caggacaaac actccaacac attattacat taccgtttaa agatttattt
     4141 gtatttattg gtggcggtgg agcgactatt ggtcttgcga tttgtctctt cctatttagt
     4201 aagagtcgtg cgaataaaac attaggtaag ctagctatta taccgtctat ttttaatatc
     4261 aatacagcta ttctatttac gtttccaaca gttttaaatc cgattatgct gattccgttt
     4321 attgctactc ctacaatcaa tgccttgatt acctatgtat caatggctgt aggattagta
     4381 ccctatacaa caggtgtaat ccttccgtgg acaatgccac cgattatagg aggcttcctt
     4441 gcaacagggg ctagttggcg aggagctcta ttacaagttg ttttgatttt ggtttctgta
     4501 gcaatttatt atccattctt caaaattgca gataaacgca atcttgaaaa agaaaaagct
     4561 actgttggag ggaaataaga tggttatcag agtatttgat caacagaaaa atacttattc
     4621 tagctttgcc ttagaggaat taagttacta tatgaatcgg gtctttaaga ctaacataga
     4681 gcttgtcgag gagaaggaag cggatatttt tgtaggatta gtcaataaag aggacagaaa
     4741 agaccatgtt cttatctcat tagacaaggg taaggggaga attgagtcta atacaattgt
     4801 aggtttactt attggaattt accgaatgtt tcatgaattt ggggttgtgt atactagacc
     4861 agggcgcgga catgactttg ttccagagtt acgatttgaa gattttttag ataaacagct
     4921 atctatagat gaaacagcca gttactatca taggggagta tgtatagagg gagcggattc
     4981 atttgaaaat atactagatt tcattgattg gctacctaag attgggatga acagtttttt
     5041 catccagttt gaaaatcctt actctttttt gaaacgttgg tatgaacatg aatttaatcc
     5101 atatctaaat aaagaacaat tttcaaatga attagtacaa gaattgagtg ataggttgga
     5161 taaagaattg caaaaaagag gtcttattca tcatcgtgtt ggtcatggat ggacaggtga
     5221 agttttaggt tactcttcaa aatttggctg ggaatcaggt cttagtattt cagaggagaa
     5281 gaaaccctat gtcgctgaaa taaacgggaa acgagaattg tttaatacgg ctccgatttt
     5341 aaccagcctg gatttttcaa atccagatgt agctgataag atggtagaaa ttatcaagga
     5401 ttatgccaag aaaagacctg atgttaacta cttacatgta tggttgtcgg atgctcgtaa
     5461 taatatttgt gaatgcgaaa actgtagaca agaattggtt tcggatcagt atattcgtat
     5521 tctcaatcaa ttggataggg ctttaacgag tgagggatta gatacaaaga tttgttttct
     5581 gctttatcat gagttgttat gggcacctca gaaagaaaaa ttagataatc ctgaacgctt
     5641 taccatgatg tttgcaccga ttacaagaac atttgaaatg agttatgcag atgtagattt
     5701 tgataattcc atacctacgc ctaaacctta tatgcgtaat aaaattatac ttccgaattc
     5761 tcttgaggaa aatttatctt atctttttga gtggcaaaaa gcatttaaag gagatagttt
     5821 cgtatatgac tatcctttag ggcgtgctca ttatggcgat ttaggctata tgaaaattag
     5881 tcaaactatt tacagagatg tatcttatct ttccaaccta catttgaacg ggtacatttc
     5941 gtgtcaagaa ttacgtgccg gattccctca taattttcct aattatgtca tggggcaaat
     6001 gctctggaag aagacaagaa gttatgaaga attgattgaa gaatactttt ctgctttgta
     6061 tggggaaaat tggcagtctg ttgttgaata tttagaaaaa ttatccattt attcctcttg
     6121 tgattatttt aatgcaattg gcagtcgtca aaatgatgtt ttagcgaatc attattatat
     6181 agcttacaat ctagctgata attttttacc aattattgag gaaaatattt ctaagttatt
     6241 aaatagtcaa aaggatgaat ggaaacagct cagttatcat cgtgaatatg ttgttaagat
     6301 ggcgaaggct ttatatcttc aagcaactgg aaaaacaagg caagctcaag atgaatggag
     6361 aaatgtgttg aattatatcc gtgggcacga attgctattt caatctaatt tggatgttta
     6421 tcgtgtaatt gaagtagcaa aaaattacgc tggtttccac ttataaatca taagtataga
     6481 aaatgaacta aggtattcag agaagattga ccctaaatat tatgaaattt aaggattttt
     6541 aagatattta gggtcgactt tctatttgta tcgtagcgaa gtcattttaa taatgatgtg
     6601 taaaagatgg atcaagattg aggaggaaga aagatgaaat caaaagaaga aataaatatg
     6661 cttggtttta caattgtcgc ttacgcagga gatgcaaggt cagatttgat ggatgctttg
     6721 gcgtttgcga gagatggata ttttgaacag gcaagagaat tggttgagtc tgcaaacgac
     6781 tcaatagtgt ctgcccatcg agaacagact aatttattag cggaggaggc atatggagat
     6841 aattttgaag tgagctttat tatgattcat ggtcaagata ctttgatgac aacgatgcta
     6901 ttgtatgatc aggtaaagtt ttttattgat gaatatgaac gaattcgaaa gattgaagaa
     6961 catattggtt tgcaatgagg attagtcatg gaaaatttac agtttaaagt cttaccgaag
     7021 gagtttttat taggagctgc taccgctgct tatcaagtag agggtgcaac tagggtagat
     7081 ggcaaaggaa taaatatgtg ggatgtttat ttgcaagaaa atagtccgtt cttaccagat
     7141 ccagctagtg atttttatta tcgttacgaa gaggatatag ctttggcggc agaacatggt
     7201 ttgcaggctt tgcgtttatc tatttcttgg gttcgtatat ttcctgatat agatggagat
     7261 gctaatgtat tagctgttca ttattaccat agagtttttc agtcttgctt aaaacataat
     7321 gtgattccgt ttgtttcttt acatcatttt gattcgcctc agaaaatgtt agaaacaggg
     7381 gattggttga atagagagaa tattgatcgt ttcatacgat atgctcgctt ttgtttccaa
     7441 gaatttacag aagtcaagca ttggtttaca atcaatgaac tgatgtctct tgctgcaggt
     7501 caatatatag gaggtcagtt tcctccaaat catcattttc aattatctga agcaattcaa
     7561 gcgaatcata atatgttgtt ggcgcatgct cttgcagtcc tcgaatttca tcaattaggg
     7621 attgagggaa aggtaggttg tattcatgct ttaaagctag gctatcctat tgatgggcaa
     7681 aaagaaaata ttttggcagc taaacggtat gatgtttata ataataaatt tctattagat
     7741 ggaacttttt tgggctacta cagtgaggac acgctttttc acttgaatca aatattggaa
     7801 gctaataatt ctagctttat tattgaagat ggtgatttag aaattatgaa gagagctgca
     7861 gctcttaata cgatgtttgg gatgaattat tatcgttcag aatttattcg tgaatacaaa
     7921 ggtgaaaata gacaagaatt taattcaaca ggaataaaag gacagtcttc ttttaaatta
     7981 aatgctctag gtgaatttgt aaaaaaacct ggtattccga caacagattg ggattggaat
     8041 atttatcctc aagggttatt tgatatgttg cttcgtatca aagaagaata tcctcaacat
     8101 ccggtcattt atttaactga aaatggtaca gcccttaaag aagttaagcc agagggcgag
     8161 aatgatatta ttgatgacag taagagaatc cgttatattg agcaacattt acacaaagtt
     8221 ttagaggctc gagatagggg agtcaatatt caaggctatt ttatatggtc tttgcaagat
     8281 caattttctt gggcgaatgg ctacaataag cgatatggtc ttttctttgt tgattatgaa
     8341 acacagaaga gatatattaa gaaaagtgct ctttgggtaa aagggctaaa acggaattaa
     8401 ggttagcgat ttgactgatg tttaatatat tttaactatg agcttaaatt ttttataaga
     8461 ggagttttat ggataagcta gtcgctgcca ttgaaaagca acaagggaaa tttgaaaaaa
     8521 tttctactaa taactatatg atggctatta aagatggatt cattgctact atgcctttaa
     8581 ttatgttttc aagctttttg atgattatta ttatgattcc taaaaatttc ggagtagagt
     8641 taccgagtcc agctattgtc tggatgagaa aagtgtatat gttaaccatg ggagttttgg
     8701 gtgttattgt ttcagggaca gttgcaaagt cattggctgg aaatgttaat agaaaaatgc
     8761 ctcctggaaa ggtaataaat gatatttctg taatgttggc agccatatgt agttatctgg
     8821 tattaactgt aacgcttgta gttgatgaga agacgggatc tacaagtttg tcgacaaact
     8881 atttaggatc tcaaggattg gtaacttcgt ttgtcagtgc ctttattact gtaaatgttt
     8941 accgattctg tattaagcga gacattacta ttcatttacc taaggaagtt cctggggcta
     9001 tatcacaagc ttttagagat atttttcctt tttcttttgt tttacttatt agtggtttgt
     9061 tagatattgt atctcggttt agtttagatg ttccttttgc ccaagtattt caacaactat
     9121 tgactcctat ttttaagggg gtagaatcat atcctgctat gatgttgatt tggtttatgt
     9181 gtgctttgct ttggtttgtt ggaattcatg gaccatctat tgtcttacct gctgttacag
     9241 ctttacaact gagcaatatg gaagagaatg ctcaacttct tgcaaatggg cagttccctt
     9301 atcattcttt aacacctaat ttcgggaatt atatcgctgc tattggagga acgggggcta
     9361 cctttgttgt accatttatt ttgattttct ttatgcggtc taaacaatta aaatcggtag
     9421 gtaaagctac aattactcct gttttatttg cggtaaatga acctcttcta tttggtatgc
     9481 ctgttatttt gaatccctat ctttttgtcc cttttttgat gactccacca gtaaatgtat
     9541 ttctaggaaa ggtctttatt gatttctttg gaatgaatgg attttatatc cagttacctt
     9601 gggcctttcc tggtcccttg ggattgttaa ttggaacgaa ttttcaatct atctcctttg
     9661 tatttttatc tttgatgtta gttgtcgaca tattgattta tttgccattc tgtagagcgt
     9721 atgatagaca gttactgatg aaagaagatg ttgtaagctc aaatgatatt attttagagg
     9781 aggagacaag tgaaataatg cctggtgaga tagatgtaat aaaaagtaag gagttgaaag
     9841 tactggttct ttgtgcaggg tctggaacaa gtgcgcaatt agccaatgca attaacgagg
     9901 gcgctcaatt agcagaagtt agagtgattg cgaattcagg agcgtacgga gctcattatg
     9961 atattatggg tgtttatgat ttaattattc tggctccaca agttcggagt tattatagag
    10021 agatgaaggt ggatgcagaa agattaggta ttcagatagt tgctaccaga ggaatggaat
    10081 atattcattt aacaaagagt ccaagtaaag ccttacaatt tgtattggag cattaccaag
    10141 ctgtgtagta agtttttcca tcttttattt gagtaaagat tttgcttaca gataggcttg
    10201 gatttaaaaa cgttccccct tttttaatat aagaatccct ctttcacaat tgtaaaaaga
    10261 gggattttgt attttatctc ttagaccaag ttctcttcat aaagagaagg aggattgggt
    10321 aaatctccaa gcgccctgca atcattgcaa aggataggag aatttttgag atgggactaa
    10381 agattgagaa actagaagtg gttcctagaa taggcccgat attattgaaa cagctaaaga
    10441 cagcgctggt cacgaccaga aaatcattgc tatctaggct gacaataaag ataagcgcta
    10501 gcaaaatcat agcatagatg acaaagtact tgagaatctt atgctgggta tctttgtcaa
    10561 tcaccgtttt attaacatgg agggtcaaaa cacggtgggg cgataggatt gacaaaattt
    10621 ggtttttggc aatttttgaa aggatgaggc ctcgaataat cttgagtcca cctgcagttg
    10681 atccagcaga gccaccgatt gccatgagga aaaggaggat aaactgggag aagaggggcc
    10741 agttggtaat atctccatat ccaaaaccag ttgttgtaat gatgttggaa acctggaaga
    10801 aggtcatttc aaagctcttt gaaaaccctg ggtagaggta gagggtgttg aggctaatca
    10861 agcctgtaga aaccagtaca atgaccaagt aagcccgaag ttcttcgtca ccaaagaagg
    10921 ccttgatgcg acggagcatg aggtagtagt agaggttgaa atttacccca aaaaccagaa
    10981 ctccgatact gaccagatag gtaatcagtg agctgccata gtgggcaatt ccgtcgttat
    11041 agacggtaaa gcctccagtt cccgctgtcc ccatagcaat gacaaaacta tcgtagagag
    11101 gcataccagc tagataatag atgatgacaa agagggagaa gagagctaga taaaggagat
    11161 agagaatctg ggcagtgttt tttagtttgg atacaacctt gccaaaaaca ggacctggaa
    11221 cctcagcctt catcacctct aggtggctat ttttggcatt gtccataata gcaagtgcaa
    11281 aaacaagcac tcccatccct ccaatcaagt gggtaaaact tcgccagaag aggagggaac
    11341 ggctgagaac cgaaacgtcg ttcaaaatac ttgctccagt agttgtaaac ccagaactaa
    11401 tttcaaaaaa ggcatcaata aggctgggga tttgcccaga aaagacaaag gggagaccac
    11461 caaagaaaga ccaaaggatc caacagaggg caacgatcaa gactccctcc ttggcataaa
    11521 tccgttgatt ttttggcttc tgtaaactcc ctgaaccgcc taacaatacg agaatcccta
    11581 tggtcgaaaa gagggctgta aagacttggc tcgattcacg gtaatagaca gcgatagcca
    11641 caggaaccaa gaggagaaca gcttcaatca aaagtaactt tgaaaggagg taacgaatca
    11701 tacttttatt catttcttac ctcgcaatca agtcataaat cttggtgatg tttggcaaca
    11761 aggttgttac taggagcttg tctccaactt ccaacatatc ctccccagtt gggaaaatag
    11821 tcttgccctt tcgaataatg gctgcaataa gaaccccttt tttcaatttc agttgagaaa
    11881 gaggtttggc agtcatttta ttggcttcct tgatatggaa ttgcagggtt tcgatttggc
    11941 cattggctag atggtgcatg gcttgaaggt ctgaatactg ggcattaact cgaccacgaa
    12001 taaagtgcat aatcgtatct acagcgatgc ttttaggtgt gatgatactt gaaaaatcag
    12061 gcgcattgat aatctcgagg agactggtac gattgacctt agtaatattt ttctgtacac
    12121 ctaccctgtc aaggaacata gatgtaatca gattttcctc atcgactcct gttagagtcg
    12181 caacggcatc atagtgttga gcactttctt ccagcaggat atcttttgcg gttccatctc
    12241 cttgaacgat gtagagattt gggaatttct cgctaaagaa gctggcgatt tcaggattga
    12301 tttcaatgac ttttgtatcg atacgactat ctttgagaat accaagtaga taataggcaa
    12361 ttctacctgc tccaacgatg agaaggctct tcacggcgcg tgatttaaaa taattatgga
    12421 agagtatcat atcgacacgg ttaccagtga caaagattct atctttatcc tgtacagtca
    12481 tgtcaccgct tggaatgata atttgatgat ccctctctat cgcacagaca atgacattac
    12541 caaatttttt acgaaaatca gaaatgggca tttggcaaag accgctggtg gacttgacga
    12601 caaattccat gaggctaacg cgtccaccag caaagcgttc gacagacagg gcgttgggga
    12661 agtcaatgat attcgcgata gcgcgggcag ccaagagctc aggattaacg ataagagaaa
    12721 aaccgagaat attcttttcc ttgaaataag agttagaata ttcagggttc cgcacccgaa
    12781 cgatagtttc tttagctccc attttcttgg ctagaactgc tgcaatcatg ttgacttcat
    12841 cgtgctcagt cagggcgata aagatatcac aatcttggac gctggcttgc tcaagaatgg
    12901 caaaatcggc cccgttacca aggataccaa tgatatcaaa gcgactgaca atatgattga
    12961 gaacagcttc gtcttgctca atcagcaaaa catcatgctt ttctgcaacc aaggagcgac
    13021 agagggcaaa accaactttt ccccctccga caaggataat tttcataata aaacctactt
    13081 tttcatgatg taactatcat accctttttc aagaaaaaat gcacctacta gctaataaca
    13141 agagctttta gtgaaaattc gctataaggt aaaactatac cctaaccaat tgaaatagct
    13201 attagcgact ttctctgaaa tatggtatga taaaggatat acaaggagat aaaatgaata
    13261 ataatttact ggtattacaa tcagactttg gtctggttga tggtgcggta tcggctatga
    13321 ttggagtggc tttagaagag tctccaacct taaaaataca tcacttgacg cacgatatca
    13381 cgccttataa tatttttgag gggagctatc gtctctttca gacggtggat tactggcctg
    13441 agggaacgac gtttgtatcg gttgtcgatc caggtgtcgg ttcgaaacgt aagagtgtag
    13501 ttgccaagac tgcaaaaaat caatacattg tcacgccaga taatgggacg ctttccttta
    13561 tcaagaaaca cgttggcatt gtagccattc gtgagatttc tgaggtggcc aataggcgtc
    13621 aaaacacaga gcattcttat accttccacg gtcgtgatgt ctatgcctat actggtgcta
    13681 aactggccag tggtcacatt acttttgagg aagtagggcc agagctcagt gtggaacaga
    13741 ttgtagagct tccagtcgta gcgaccatca tagaagatca tctggtgaag ggagccattg
    13801 atattctgga tgtgcgtttc ggttcgcttt ggacctctat cacacgggaa gaattttaca
    13861 agctggaacc agaatttggt gatcgttttg aagtgaccat ctatcatgct gatatgctgg
    13921 tctatcaaaa tcaggttgtc tatggcaaat catttgcaga tgtgagaatt gggcaaccca
    13981 tcctttacat caactctctc tatcgtttag gtttggctat caaccaaggt tcctttgcca
    14041 aggcctataa tgtaggtgtc ggttcatctt ggaccattga aataaagaaa atagaaggat
    14101 aaaataggag aatagtatgg aaatcaaatt tacaattaaa caagttgttg ctgtcggaat
    14161 tggcgctgcc ctctttgtcg tcatcgggat gatcaacatt ccaacccctg ttccaaatac
    14221 aagcatccag cttcagtatg cggtacaagc gctactttct attatttttg gaccgattat
    14281 cggtttgctt gtcgggttga ttggtcatgc aatcaaggac tctcttgttg gttatggtct
    14341 atggtggact tggattatcg ctagcggact ctttggttta gttgtgggac tttttagaaa
    14401 gtacgttcgc gtgatcaatg gtgtttttga ctggaaagat attcttattt ttaatctcat
    14461 tcaactactt gcaaatgccc ttgtttgggg tgtcttggca ccacttggag atgttgtgat
    14521 ttatcaagaa gcggcagaaa aagtatttgc acaagggatt gttgcgggaa ttgccaatgg
    14581 tgtatctgta gctattgcag gaactcttct cttacttgcc tatgcaggaa ctcaaactcg
    14641 tgcaggaagt ttgaaaaagg actaatactc aatgaaaatc aaagagcaaa ctaggaagtt
    14701 agccgcaggt tgctcaaaac agtgttttga aattgtggat a
//
LOCUS       DAVAAL010000040        13977 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVAAL010000040 DAVAAL010000000
VERSION     DAVAAL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.041"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            152..451
                     /locus_tag="VQM60_001021"
     CDS             152..451
                     /locus_tag="VQM60_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET1080901.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSIVSRWNGHSDKGEAPAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            522..2543
                     /locus_tag="VQM60_001022"
     CDS             522..2543
                     /locus_tag="VQM60_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET1080902.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFIYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDIENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNILYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            2545..3186
                     /locus_tag="VQM60_001023"
     CDS             2545..3186
                     /locus_tag="VQM60_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080903.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3290..4642
                     /locus_tag="VQM60_001024"
     CDS             3290..4642
                     /locus_tag="VQM60_001024"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET1080904.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIATD
                     KNTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMAAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4644..5702
                     /locus_tag="VQM60_001025"
     CDS             4644..5702
                     /locus_tag="VQM60_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET1080905.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5712..6932
                     /gene="divIB"
                     /locus_tag="VQM60_001026"
     CDS             5712..6932
                     /gene="divIB"
                     /locus_tag="VQM60_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET1080906.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEKSEEKVASSEADKEKEEKEESES
                     KEKEEQDKKLAKKAIKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKAFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKRLEEEQKKQEE
                     ESNRNQTTQRSSRR"
     gene            7165..7866
                     /gene="pyrF"
                     /locus_tag="VQM60_001027"
     CDS             7165..7866
                     /gene="pyrF"
                     /locus_tag="VQM60_001027"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET1080907.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            7900..8532
                     /locus_tag="VQM60_001028"
     CDS             7900..8532
                     /locus_tag="VQM60_001028"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET1080908.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            8712..9115
                     /locus_tag="VQM60_001029"
                     /pseudo
     CDS             8712..9115
                     /locus_tag="VQM60_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9211..9975
                     /locus_tag="VQM60_001030"
     CDS             9211..9975
                     /locus_tag="VQM60_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080909.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            9975..10727
                     /locus_tag="VQM60_001031"
     CDS             9975..10727
                     /locus_tag="VQM60_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080910.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            10737..11435
                     /locus_tag="VQM60_001032"
     CDS             10737..11435
                     /locus_tag="VQM60_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080911.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            11432..12079
                     /locus_tag="VQM60_001033"
     CDS             11432..12079
                     /locus_tag="VQM60_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1080912.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(12159..13024)
                     /locus_tag="VQM60_001034"
                     /pseudo
     CDS             complement(12159..13024)
                     /locus_tag="VQM60_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(13036..13793)
                     /locus_tag="VQM60_001035"
                     /pseudo
     CDS             complement(13036..13793)
                     /locus_tag="VQM60_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(13794..>13977)
                     /locus_tag="VQM60_001036"
     CDS             complement(13794..>13977)
                     /locus_tag="VQM60_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine ABC transporter permease"
                     /protein_id="HET1080913.1"
                     /translation="LVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVC
                     YPISKLSTHLEKHWRN"
BASE COUNT         4165 a         2377 c         3021 g         4414 t
ORIGIN      
        1 aaatatctaa aaaattcgat tttttccttg acaacaaata ataaaaatgt tattcttatc
       61 acataagaaa gcggtttcaa caaaagagag aggaggattt attttgttgc attgttgctc
      121 agggtgtgct ttttttgaaa gaaaaaataa aatgaaaacg aaaaaacata gattacttgc
      181 tctagctctt atttcaagtt ttacattatt gggagctgca tcagctgctg tacaatatcc
      241 agatggagga gtatggacat atggagaagg ttcaggaggt ggttgggctt tttcaaatta
      301 ctatcatggt aaaaaatatc attattcatc catcgtgagc agatggaatg gtcattctga
      361 taaaggagaa gctcctgctg gaaaaacctc ctatgcatgg atttggacta aatggggaga
      421 acaagtagca ttttactgtg attatgacta atcgttcatt gatttaaatc gttccccttg
      481 taagaaatta cagggggaat tttgtttaca aggagtctac tatgaagaaa ctattcatat
      541 tattatcaac tttttttctc agcttcttcc ttgcttggat tattgtctta cgtgcgccac
      601 aatatttata tgcaagctat gattccgttt ctttacttcg tgtcaaaaaa gatactcagg
      661 aaccgacgcg tgaggtattt gaacaggaat tggagaattt tgcaaattca gaacagagtt
      721 taatagctag aagaattgta gagccgagta aggatgggac gactcacttt atttatgcaa
      781 cttatggtca gggaacttta ccaaaagaat tccaagaagc tagccaagaa agtcgtgaac
      841 gtagtgatcc gctaaatagt tatctccttt tgtcaggttc cttgacgaaa gaaaagcttg
      901 ccgataaatt aggagatttg ggttataaag caagtgctga ccgaaagata ccgccctatt
      961 ttcttgcttt tcgaatatta ctaaatcccc ttattttaat tagtttagca atatttggct
     1021 tatctttctt tgctttagtg attatcactc ggattaagga aatgagagca gcaggtataa
     1081 aactcttttc tggtcagact ctcttatcca tcatggggca ttctttatct actgatatca
     1141 aatggctcct tctatcagcc ctcctttcct tcctaggtgg gggagtcgtt ctttttagtc
     1201 aaggtttgtt ttatcctatc ttgttagcca cctatggttt tgggattagt ttctatctgt
     1261 tgtttttatt ggcgatttca attttactaa tgctccttta tctaatgagt ttgaattaca
     1321 aagcattagt tcccgttatt aaggggagat taccccttaa acgcctgatg attttaaccc
     1381 tattgtgtca gttggttgct gtctttacag taggatacgc tgttaagaca ggtttgacgt
     1441 cttaccaacg attgaaagaa cttgaaattt caaaacaagc atggcaggat agagcagact
     1501 attatcaaat ttcttttggc ttaggtgata gaggaaaaga tatagaaaat cagagcaagt
     1561 ggtatgcctt tgccaaggaa gcaatcgaag aagaacaagc tctttatgta aaggataatc
     1621 tgctccattt tgccaatcca caaggaaaaa atgaacaggg agagacactg gatacctata
     1681 gtccagatgc taatacgctc tatgttagtc ccagttattt ggacaaggaa aaggtcgtgg
     1741 tagatgctga gaccaaacag aagttagccc atctccaaaa aggtgagttt atcctcttgc
     1801 tcccagaaca tttgcgctct cgagaagcag aacttaagaa agtttttgaa gaaagattga
     1861 gttattatgg aaaatctggt gaggaggcaa gtgctccttt ggattatgag atgaaagcgc
     1921 acgttagtta tctttcaatg ggagaaaagc ggtttgttta taataacggt gagaatcccg
     1981 tatccactca gtatttgact gatccgattt tagtggtatt cacgccgact tctacaggtg
     2041 atagttttat atccttatct agttggtcta tcaatgctgg aaaacaactc tttatcaaag
     2101 gatatgagag tgggctagaa ctcttgaaga aagctggaat ttatgagcaa gtatcctatc
     2161 ttaaagaagg aagaagtgtt tatctaactc gttataatga agttcaaact gaaacagcaa
     2221 ctttaatctt aggagctatt gtggggatag ctagttcctt gttactattt tattctgtca
     2281 atattctata tttcgagcaa ttccgccgag atatcttgat taaacgaatt tcaggtttac
     2341 gattttttga aacacatgct cagtatatgg ttagccaatt tgccagtttt gtatttggtg
     2401 ctagtctctt tattttaagc agtcgagact tggtgattgg cttgctcact ttattggtct
     2461 ttctagctag tgcagttttg acgctttacc gtcaagcgca gaaagaatct cgtgtttcta
     2521 tgacaattat gaaaggaaaa taggatgatt gaactaaaga atatatctaa aaaatttgga
     2581 agccgtcagc tattttcaga tacgaatctt cattttgaag gtgggaaaat ttatgcctta
     2641 atcggtacaa gtggctgtgg taagacaaca ctcttgaata tgattggacg attggagcca
     2701 tatgacaaag ggcaaatcat ctatgatggc acttctctta aggacatcaa gccttctgtt
     2761 ttctttagag attacttggg atacttattt caagattttg gcttaattga aagccaaacc
     2821 gtcaaagaga atctcaatct gggtttagtt ggtaaaaagt tgaaggaaaa agagaaaatc
     2881 tctttgatga aacaagctct aaaccgtgta aacctctctt atttggattt gaagcaacct
     2941 atatttgagt tatcaggagg agaagcacaa cgtgttgcac tagcgaagat aattttaaag
     3001 gatccgcctt tgattcttgc agatgaacca accgcttcct tagaccccaa aaactctgag
     3061 gaattacttt ccatcctaga atctttaaaa aatccgaatc ggaccattat tattgcgacc
     3121 cacaatcctc tgatttggga gcaagtggat caggtcattc gagttaccga tttatcacat
     3181 agatgatatg gtaagattca gttagaagaa agagtcacaa acacactttg tggctttttt
     3241 atttccataa aaatggtaaa atagtaggag tagaaatgga gttcgagaca tgaaagtaat
     3301 agatcaattt aaaaataaga aagttcttgt tttaggtttg gccaagtctg gtgaatctgc
     3361 ggctcgtttg ttggacaagt taggtgccat tgtgacagta aatgatggga aaccattcga
     3421 ggacaatcca gctgcccaat gtttgctgga agaagggatc aaggtcatta caggtggcca
     3481 tcctttggaa ctcttggatg aagagtttgc ccttatggtg aaaaatccag gtatccccta
     3541 caacaatccc atgattgaaa aggctttggc caagggaatt ccagtcttga ctgaggtgga
     3601 attggcttat ttgatttcag aagcaccgat tattggtatc acaggatcga acggtaagac
     3661 aaccacaacg actatgattg gggaagtttt gactgctgct gggcaacatg gtcttttatc
     3721 aggaaatatc ggctatcctg ctagtcaggt tgctcaaata gcgacagaca agaacacgct
     3781 tgttatggaa ctttcttctt tccaactcat gggtgttcaa gaattccatc ctgagattgc
     3841 ggttattact aaccttatgc caactcatat cgattatcat ggattatttg aggaatatgt
     3901 agcagccaag tggaatatcc agaacaagat ggcagcagct gatttccttg tcttgaactt
     3961 taatcaagac ttggcaaaag acttggcttc caagacagaa gccactgttg taccattttc
     4021 aacacttgaa aaggttgatg gagcttatct ggaagatggt caactctact tccgtggtga
     4081 agtagtcatg gcagcgaatg aaatcggtgt tccaggtagc cacaatgtgg aaaatgccct
     4141 tgcgactatt gctgtagcca agcttcgtgg tgtggacaat caaaccatca aggaaactct
     4201 ttcagccttc ggtggtgtca aacatcgtct ccagtttgtg gatgacatca agggtgttaa
     4261 attctataac gacagtaaat caactaatat cttggctact caaaaagcct tgtcaggatt
     4321 tgacaacagc aaggtcgtct tgattgcagg tggtttggac cgtggcaatg agtttgacga
     4381 attggtgcca gacattactg gactcaagaa gatggtcatc ctgggtcaat ctgcagaacg
     4441 tgtcaaacgg gcagcagaca aggctggtgt cgcttatgtg gaggcgacag atattgcaga
     4501 tgcgacccgc aaggcctatg agcttgcgac tcaaggagat gtggttcttc ttagtcctgc
     4561 caatgccagc tgggatatgt atgctaactt tgaagtacgt ggcgacctct ttatcgacac
     4621 agtagcggag ttaaaagaat aatatgaaaa aaattgtctt tacaggtggg gggacggttg
     4681 gacacgttac cctcaatctt ttgttaatgc ccaagttcat cgaagatggt tgggaagtcc
     4741 actatatcgg ggacaagcgt ggtattgaac accaagaaat ccttaagtca ggtttggatg
     4801 tcaccttcca ttctattgcg actggaaaat tgcgtcgcta tttctcttgg caaaatatgc
     4861 tggacgtctt caaagttggc tggggaatcg tccaatcgct ctttatcatg ttgcgacttc
     4921 gtccacagac ccttttttca aaggggggct ttgtctcagt accgcctgtt atcgctgcgc
     4981 gtgtgtcagg agtgcctgtc tttattcacg aatctgacct gtctatgggc ttggccaata
     5041 aaatcgccta taagtttgcg actaagatgt attcaacctt tgagcaagct tcgagtttgt
     5101 ctaaggttga gcatgtggga gcagtgacca aggtttcaga tcaaaaaaat ccagaaccag
     5161 atgaattggt ggatattcaa agccacttta atcataaatt gccgactgta ttgtttgttg
     5221 gcggttctgc aggtgctcgt gtctttaacc aattggtgac agaccataag aaagaactaa
     5281 cagagcgcta caatattatc aatctaactg gagattctag cctgaatgag ttgagccaaa
     5341 atctttttcg tgttgactat gtgaccgatc tctatcaacc cttgatggaa ttggctgata
     5401 ttgttgtgac acgtggtggt gccaatacga tttttgagct cttggcgata gcaaaattgc
     5461 atgtcattgt gccacttggt cgtgaagcta gtcgtggtga ccagattgaa aatgcagctt
     5521 actttgttaa gaaaggctat gcagaagacc ttcaagaaag cgatttgacc ttggatagtt
     5581 tggaagagaa gctttctcac ttactaagtc acaaggaaga ttaccaagct aagatgaaag
     5641 cttctaagga attgaaatct ctagcagatt tttatcaatt gttgaaaaaa gatttatcat
     5701 aaggaaagta aatgtcaaaa gataagaaaa atgaggacaa agaaatcctc gaagaattga
     5761 aagagttatc agaatggcag aaacgaaacc aagaatatct aaaaaagaag gctgaagaag
     5821 aagcggctct agctgaggag aaggaaaagg aaagacaagc tcggatggga gaagaatctg
     5881 agaagtcaga ggacaaacag gaccaggaga gtgaaacaga ccaggaagat tcagaatcag
     5941 ctaaggaaaa gtctgaagaa aaagtagcat cctcagaggc tgacaaagag aaagaagaga
     6001 aagaagaatc agagtctaaa gagaaggagg aacaggataa aaagcttgct aaaaaggcta
     6061 taaaggaaaa accagccaaa gcaaagattc ctggtatcca tatcttgcga gccttcacga
     6121 ttttatttcc aagtctgctt ttattgattg tctctgccta cttgctcagt ccttatgcga
     6181 ccatgaaaga tattcgtgtt gagggaacgg tgcaaactac agctgatgat attcgacagg
     6241 cttcaggcat tcaggattcg gattatacga ttaaccttct gctagacaag gcaaaatatg
     6301 aaaagcagat taagtctaac tattgggttg aatcagctca acttgtctat caatttccaa
     6361 ctaagttcac tattaaggtc aaggaatatg atattgtggc ctactatatt tctggtgaaa
     6421 atcattatcc tattctttcc agtggtcagc ttgagactag ttctgtgagc ctgaacagtt
     6481 taccagaaac ttatttatca gttctcttta atgatagtga acaaatcaag gcttttgtct
     6541 cagaacttgc tcaaattagc ccagaactca aggcggctat ccaaaaggtg gaattagccc
     6601 caagcaaggt gacatccgat ttaattcgat tgaccatgaa tgattcggac gaagtcttgg
     6661 ttcctctatc tgaaatgagt aagaaactgc cttattatag taagattaaa ccacaattgt
     6721 cagaaccgag tgtgattgac atggaagctg gaatttacag ttacactgtg gcggataaat
     6781 taattatgga agctgaggaa aaagccaaac aagaggctaa ggaagccgag aaaaaacagg
     6841 aagaagaaca gaaacgttta gaagaagaac agaaaaaaca agaggaagag agcaatcgaa
     6901 atcaaacgac ccagcgctca tcgcgtcgct aggtttacct tttctcttat agttctttag
     6961 tgaccatgtt tttacttgac aagtgctagt agaaataata gaatagtaaa aaacctttaa
     7021 agcagtccag agaggcagct aaggttagac ggtgaaaggg tggagactac ccatttttcg
     7081 tggaaccttg ctgttggcag gttccttttt tcgtggcttc tgttggccag actctctcac
     7141 tagtaaaggt aaaaggagaa acctatgcga gaacatcgtc caatcattgc tcttgatttt
     7201 cctagttttg aggcggtcaa ggaattttta gctcttttcc cagcagaaga aagcctttat
     7261 ctcaaggtag ggatggagct ttattacgca gcggggcctg agattgtgtc ctacttaaaa
     7321 ggtttgggtc atagtgtctt tttggatctc aaacttcatg acattcctaa tacagtcaag
     7381 tcagccatga aggtcttgtc tcagcttggt gtggatatga ccaatgtcca tgcggctggt
     7441 ggtgtagaga tgatgaaggc ggcgcgtgaa ggtcttggga gtcaagccaa attgatcgct
     7501 gtaacgcagc tcacatcaac gtcagaagct cagatgcagg agtttcaaaa tatccaaacc
     7561 agtctgcaag agtctgtgat tcactatgcc aagaagacag ctgaagctgg cttggatggt
     7621 gttgtttgct cggctcagga agtacaagtc atcaagcagg ctaccaatcc agattttatc
     7681 tgtctgacac cagggattcg tccagctggt gttgcagttg gagatcaaaa acgagtcatg
     7741 acacctgctg atgcctatca aatcggcagt gactatatcg tagtgggacg tcccattacc
     7801 caagctgagg atcctgttgc agcttatcat gccatcaagg atgaatggac acaggactgg
     7861 aattaaagaa ctagattaga aaaataaaag gagaatacca tgacacttgc taaagatatc
     7921 gctagccacc tcttgaaaat ccaagccgtt tacctcaaac cagaggaacc cttcacttgg
     7981 gcatctggta tcaagtcacc gatttacact gataatcgtg tgacactagc ctatccagaa
     8041 actcgtaccc taattgaaaa tggttttgtg gaagctatca aagaagcctt tcctgaagta
     8101 gaagtgattg caggaactgc aacagcaggg attccacacg gagccattat tgctgataag
     8161 atggacttgc cttttgccta cattcgtagc aaaccaaaag accacggagc tggtaatcaa
     8221 atcgaaggtc gcgtagctca aggtcaaaaa atggtagtgg ttgaagacct tatttcaacg
     8281 ggtggttcag ttcttgaagc tgtagcagca gccaagcgag aaggagcaga tgtacttgga
     8341 gttgtagcga ttttcagcta ccaattgcca aaagcagata agaactttgc agatgctggt
     8401 gttaaacttg tgacgctttc aaactatagc gagcttatcc atctagccca agaagaaggt
     8461 tacatcacgc cagagggcct tgatcttcta aaacgcttta aagaagacca agaaaattgg
     8521 caagaaggtt aggtcagtaa gataaagaga gacgaggcta ccgagtctct tttaccattt
     8581 tatttaaaat atgacaatat tttaaataaa ataattgaca acgctttctt ttgttgatat
     8641 aatgaaaatg taatatgtat caaattgatc aatcaactat cttgtttatc acttcttaag
     8701 aggacgatgt catgaaaaga agaaaaatat attccttttt tgcgtagtta caattgcatc
     8761 tagtttgttt ctccctacag ttttagctag acagattgga aattatgatt taacgttgcc
     8821 gcgttggggt tcggatacaa ctagtgagct tgagaaagaa aacgcctctg ctggaattaa
     8881 taatagtgac agcactggtg gcggtaaaag gttaaatacc tctattcgta gcgcctatag
     8941 tgggtcagat attaccccgg tatattcatt ggggtctggc tctaggattg tcatgtacta
     9001 taatggaggt ggtgacaatt atattggttc tggtactaga ttagctatgg cgccacaatt
     9061 tggaaatcat gtaagaattc atacttcagg ttcttggagt ccagattctt attaacttac
     9121 ttgtcagagt aagccttaaa gatggttgat tgtgggtgta gcatgaaaaa agaatgctac
     9181 accctatttt tattataagg aggagtaagg atggaatttt tcatttgtaa tcttgtacga
     9241 gtcgttcaat cacctcggtt ttatatgtct ttatttttga cccttctttg catgagttta
     9301 ggaaattttc ttgctttcaa tggtatttat aaaattgaag gtttatcgat tttttttgcc
     9361 gcttcttcta ttcgaggatt ttcaccgatt agcctagtag ctgcacttat ctgtacactg
     9421 ccctattcta gtcagataat agaggatgct gagagtcatt ttctaacagc acaattgtgc
     9481 cgaatttcta aaaagaggta tctagctatt gtaggtagta ctgcaattat ttcttctttt
     9541 ctagtctttt ttctccccta tttattatta ttaggaatta atcttttagt gactccttat
     9601 caggaaattt atattggaga ttatagtggt gccttaaaag aattatttga ttctaatcag
     9661 tttctctata gtcttgtgac gactctctgg tatggagttt ggggcgctgt gttctctatt
     9721 tttggactag ctagtgcttt gctagtgaag aaaaaattag gagctatttt catcccagtt
     9781 gcctatatga tggttggtgg tattttatgg gctattttag ggctatctta cttagaacct
     9841 gtgacaacgc tagctttggg atatcagaaa gatatcagtc tttccttagt tagtgggcat
     9901 cttgctttta ttttatttgt tagttgtttg gttgtttatg gtacattttt tctacattca
     9961 gaggactatg tataatgaaa caatttgttc aattttataa aaaagatttc ttagcagtat
    10021 tggtttattt tatattactg ctatcctgtg ttttatctag tacagtatat ttattgcgct
    10081 gtcgccaata ttcaatccat ccaaatgtat tagaatggat cttagtttta cttcaagata
    10141 tgacgactgg agtatattgc tttccgttca catatatatt gttctttttt tatttgatga
    10201 ataactattt taataggttg gagtgtcgca ttcgtctgaa atcaattaag cactttacca
    10261 gttttagttt caaattagca gctcttagta cggggatttg gacggcgact ttatttttat
    10321 tgatttttct aattgcattt agtaatggtt ttagcttctc tttggagata aaggaggttg
    10381 attttttaag agaattttat ggtataagta ttgcaaacaa tgctagtttc tttataggat
    10441 tttttttctc ttatatagca tactatttct ttttatcctt acttactatt agcagttttt
    10501 cttggtttaa aaaatcaaac atgagcttag tatttctgtt tactttttta tttgtagaat
    10561 ccttattctg gatttatcag ttggacaatg ggataattgg attattgcca atttttcagt
    10621 atatggtaaa ttccaatccg tatgcattga tttattggct tacattacta tctatcataa
    10681 ttccattgac tgtattttct gttcatagaa actggaggag agtgtaaaag ttggaaatgg
    10741 gaaagttaag tagtcacatg tggaggttga atcagataat ctataccaag tacttttggg
    10801 gttatgttct tttttggata ttgatttgtt taggattatg gtattggtta gaaggaaatg
    10861 atagacttgt tatagaaatt ttaaaagggc ctaatctgag tcaaaactct tttttagtct
    10921 tatctatatg gttgcttcat tggtttatta ttcatacatt ttttctagca gttgtatatc
    10981 gtagaagagc atccgatttc tttatggaag tgattcgatt ttcttctatt aagctctgga
    11041 ttaggtatca gatttggacc tgttttcttt atggactcat tttaatcatg gtaaaagttc
    11101 tagtgattca atttatgtta cagttaccaa actgggatat aggagttttg tttatagttg
    11161 attctttgaa tgcttgtgtg ttagtcttgt tttgctttat gttatacgca ctaggagcga
    11221 atgtacaaat gaactttgct tgcgttagtt tctttttact catgattgtg tttggtggtc
    11281 tatttgtagg aaatcgaact aattatctat tttatatttt gaatcgaggg aatggagata
    11341 taggacgaga tttatttctt cagttgctat ttttagtctt tctttttaaa agtattttct
    11401 atttcactcg tcaaaagagg aggtttatag aatgaatgaa ataatcatat taaaaaatat
    11461 tgagttgaaa ttaaaaaaaa catgtgtttt tcaaaacctt aattttagtt gtaaacaggg
    11521 ggaaattata ggaattactg gtgcgaatgg ctcagggaaa agtgtattgt ttaaattaat
    11581 agctggttta tatagtccgt cttatggaga agtgttaatc aatggggaaa atattgttcc
    11641 tgagagaaaa attccagcta atttgggagc tttgattgaa gaacctggtt ttataaatta
    11701 ttatagtggc tttaagaatt tacaatattt ggcaagcata cgaggagtag ttggtaatca
    11761 ggaaatcaat gatacactga aaatagttgg tctatatgag caaaaagacc agaaagttaa
    11821 aacttattcg ctaggtatga ggaaaaagct agggattgct caagcaatta tggagaatcc
    11881 ctctattctt ttactagatg aacctatgaa tgccttggat aaatcaagtg tagaaaatat
    11941 gagaacattg tttagaaagc tctctagtga aaaagggaca acaattttga ttgctagtca
    12001 cagtgaagag gatattcgta tcttatgtga taaagtatat gcaatagaag ataaagtatg
    12061 tacactgtgt tcagattgaa catcttagat agactcagga tgttataggt aaaatcttaa
    12121 aaagggattg gagcttaaaa tccagtccct tttaagagtt aatggccacc ttcaacaacc
    12181 aggtcatctg ctttagcagc gtcaccgtag gttgggtgaa gtgttttttc ataggccttg
    12241 tggaagaagt tttccttgcc taatttttca atatctttat tgatgaagtc tagcaattct
    12301 tggttgcctt tttgaactgc tgccgcaatg gtatcgggat caccgaggga agtaattcct
    12361 acttcaaatc ctttattttc aagcgcccaa gctagaactt ccgtattgtc agttgaaaag
    12421 gcatctccac gtccgtcaag aagagcttgg taagagtcac tgtattggtc gtatttttgg
    12481 agtttgattt ctggatgatt cttttcaaaa taagtctcag cagtcgttcc ttttgtgaca
    12541 attaaggttt taccttcaag ttgtttgacg tctgtaatga gaccagtctt aggtgatacg
    12601 acacccagag aaactttcat atatggaagg gcaaaatcaa cttgtttctt acgttcgtca
    12661 gttactgtaa agttagcaag agtaatatct accttgtttg aaatcaagta ttccgcacgg
    12721 ttgtcagcat cgactgaaat gtatttaacc ttgacaccaa ggtcttgagc tagttggttc
    12781 cctagttcaa tatcgtagcg tagccttggt aagaaccatc attgtcaacg tagccaaacg
    12841 gttttttatc tccaaacacg gcgattcgca gttcaccgct ttttttgatt tcatcgatag
    12901 tgcgagcttt ggcagttgtt tttccagagg atgaaccagc gtttccacct gagctacaag
    12961 cagtgataaa gataagggca aaggcaagtg ctaaaacagt taagagtggt ttgaatagtt
    13021 tcataagaat ctcctttata gatatgagcc gaattggcta aagtcaaaga cgtttaaaaa
    13081 ttcctgggct cgtttggttt gcggattggt aaagaaggct tgagctgttc cttcttcagc
    13141 gattttccct tggtcgagga agataatctg gtcagcaatg gcttgggcaa actgcatttc
    13201 gtgggttact aaaatcatgg tacggccttc ttgggccaaa tcattgataa gttccagcac
    13261 ctcacgcacc atttctggat ccagcgaagc agtcacctcg tcaaaaagga tgatttctgg
    13321 atgcattagg agggcacgga caattgcaac acgttgcttc tgtccaccag ataattgacg
    13381 ggcaaagcta tgttgtttat ccagcaaacc gacacgatct agtaattgca aagcttcttc
    13441 cgttacttct ttcttgtccc ttccttgagc tttgataggg cctaggatga ggttttgtaa
    13501 gacatccaga tggggaaaga gttcataact ttgaaagacc atgccaatct tttggcgaac
    13561 taggtgaaaa tcttttttat tttcaacgat agactgacca tccagaagaa tatctccacc
    13621 ttgaatactt tctaagccgt tgaggcaacg aaggagggta cttttcccac aaccagaggg
    13681 ccctaggatg acaacaactt cccttttttg atttctagag aaagtccttg gaggatggga
    13741 ttgtctccga aggatttttt tagttccttg atttctaaga tagtttcaga catttagttt
    13801 ctccaatgtt tttctaagtg agtggatagt ttggaaatag ggtagcaaac tgcgaaatat
    13861 aagactagaa tggttccata aatccaaaat gaagcagttg ggatggtcag gcgattgcta
    13921 tcgatgattt gttgtccaac tttggtcact tccacaaccc caatcaaaac aactaat
//
LOCUS       DAVAAL010000041        13772 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVAAL010000041 DAVAAL010000000
VERSION     DAVAAL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.042"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..445
                     /locus_tag="VQM60_001037"
     CDS             <1..445
                     /locus_tag="VQM60_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET1080914.1"
                     /translation="FPGLTVMENLEMGAFLKKNREENQANLKKVFSRFPRLEERKNQD
                     AATLSGGEQQMLAMGRALMSTPKLLLLDEPSMGLAPIFIQEIFDIIQDIQKQGTTVLL
                     IEQNANKALAISNRGYVLETGKIVLSGTGKELASSEEVRKAYLGG"
     gene            859..1515
                     /locus_tag="VQM60_001038"
     CDS             859..1515
                     /locus_tag="VQM60_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET1080915.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNYQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            1623..2718
                     /gene="prfB"
                     /locus_tag="VQM60_001039"
     CDS             join(1623..1694,1696..2718)
                     /gene="prfB"
                     /locus_tag="VQM60_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET1080916.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            2736..3428
                     /gene="ftsE"
                     /locus_tag="VQM60_001040"
     CDS             2736..3428
                     /gene="ftsE"
                     /locus_tag="VQM60_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET1080917.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            3421..4347
                     /locus_tag="VQM60_001041"
     CDS             3421..4347
                     /locus_tag="VQM60_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1080918.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLRI"
     gene            4633..6813
                     /locus_tag="VQM60_001042"
     CDS             4633..6813
                     /locus_tag="VQM60_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET1080919.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTLFGGSIKVADYFISVMEAPALNMGVFVGIISGFIGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSIIAAIVLAAFWPVVQTGINSFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVLTGAAKGSQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVVVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFVATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEESSNEVKVAAGSQAVNIINLLGGRANIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADVLKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKGLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHALGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVVAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            6865..7680
                     /locus_tag="VQM60_001043"
     CDS             6865..7680
                     /locus_tag="VQM60_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET1080920.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEI
                     TSPEVEVNDLYQALPAAELIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            7820..9163
                     /locus_tag="VQM60_001044"
     CDS             7820..9163
                     /locus_tag="VQM60_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET1080921.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKIVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            9540..10730
                     /locus_tag="VQM60_001045"
     CDS             9540..10730
                     /locus_tag="VQM60_001045"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET1080922.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            11283..12218
                     /locus_tag="VQM60_001046"
     CDS             11283..12218
                     /locus_tag="VQM60_001046"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET1080923.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            12252..13289
                     /locus_tag="VQM60_001047"
     CDS             12252..13289
                     /locus_tag="VQM60_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET1080924.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            13430..>13772
                     /locus_tag="VQM60_001048"
     CDS             13430..>13772
                     /locus_tag="VQM60_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET1080925.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQ"
BASE COUNT         4227 a         2448 c         3028 g         4069 t
ORIGIN      
        1 ctttcctggc ttgactgtta tggaaaatct tgaaatggga gctttcttaa agaaaaatcg
       61 tgaagaaaat caagctaact tgaagaaggt tttctcacgc tttcctcgtc ttgaagaacg
      121 taagaaccaa gatgcagcta ctctttcagg aggggaacaa caaatgcttg ccatgggacg
      181 cgctcttatg tcaacaccaa aacttcttct tttagatgaa ccatcaatgg gacttgcccc
      241 aatcttcatc caagagattt ttgatatcat tcaagatatt cagaagcaag gaacaaccgt
      301 cctcttgatt gaacaaaatg ccaataaagc acttgcaatc tctaaccgag gatatgtact
      361 ggaaacaggg aaaatcgtcc tatcaggaac aggaaaagaa ctcgcctcat cagaagaagt
      421 cagaaaagca tatctaggtg gctaaaacaa tccagtggat tgttttagtc ggcagatgaa
      481 gattacgaag taatcatcat tatagtctgg gagactagtt taggttgcag ataaagattg
      541 cgaagcaatc atcaaatcca gaggattgtt ttagccggca gatggagatt acgaagtaat
      601 catcaatata gtccggggga cctttttagt cggtggatag agattgcaaa caaatatgca
      661 tctacattga aagcttaatt tctaataatt gaaaaaatcg aatgaaaaat ttcttacctt
      721 cattcacaga gctctatttc agagctcttt ttgctagctt attcatactt ttttgaattt
      781 cgaaaaagaa atgtaagcgt ttgatagatt tacaaaaaga ttgtataatg gggataagaa
      841 tagaaaagga gaagtctcat ggcagttaaa gattttatga cccgcaaggt agtttatatt
      901 agtccagata caacagtatc tcatgcagca gatttgatga gagagcaagg tttgcaccgt
      961 ctgcctgtta tcgaaaatga tcaattagtt ggtttggtga ctgagggaac cattgcacaa
     1021 gcaagtccat ctaaagcaac aagtctttct atctatgaga tgaattatct tctgaataag
     1081 acaaaagtaa aagatgtcat gattcgcgat gttgtcactg tctcaggcta tgctagtcta
     1141 gaagatgcaa cttatctgat gttgaaaaat aagattggta ttctccctgt cgtagataac
     1201 tatcaagtat acggagttat tactgaccgt gatgttttcc aagcctttct tgaaattgca
     1261 ggttatggcg aagaagggat tcgtgtacgc tttgttacag aagatgaagt tggtgttctt
     1321 ggaaaaattg tttctttgat tgtagaagaa aatttgaata tctcccatac agtcaatatt
     1381 ccgcgtaagg atggtaaggt gattatcgaa gtgcaaatcg atgaatcaat tgatttacca
     1441 gccttgaaag aaaaatttga agcaaatggt attcaagtgg aagaaatcgc tcgcacttca
     1501 gcaaaagtct tgtaagaagg gaagtccaaa ggcttctttt ttcatgaaaa ggggattaga
     1561 gcaaaagatg gaaagaaatg ataaaatatg ctataatgaa ataatgtaaa aaaggagtat
     1621 ttatggacat ttcagtaatt cgtcagaaaa ttgacgcaaa tcgtgaaaaa ttagcttctt
     1681 tcagggggtc tctttgacct cgaagggcta gaggaagaga ttgccatctt ggaaaacaag
     1741 atgacagaac ctgatttttg gaacgataat attgcggccc aaaaaacgtc gcaagaattg
     1801 aatgaattaa aaaacactta caataccttc cataagatgg aagagttgca ggatgaagtc
     1861 gaaattttat tggatttttt ggctgaagac gagtcagtgc atgatgaact ggtagcgcag
     1921 ttagccgaac ttgataagat aatgaccagc tacgagatga ctctactctt gtcagaacct
     1981 tatgaccaca acaatgccat cttggaaatc catccaggtt ctggtggtac tgaggcgcag
     2041 gactggggtg atatgttgct tcgtatgtat actcgttatg gtaatgctaa aggctttaaa
     2101 gtggaagtgt tggattacca agcaggtgat gaggctggta ttaagtcggt aactttatca
     2161 tttgaagggc ctaatgccta tggtctcctc aagtcagaaa tgggtgttca ccgcttagtg
     2221 cgaatctcac catttgactc tgccaaacgt cgccatacct ctttcacatc tgtagaagtg
     2281 atgccagaat tggatgatac tattgaagtg gaaatccgtg aagatgatat caagatggat
     2341 accttccgtt caggtggtgc cggtggacaa aacgtcaata aggtttcaac aggtgtacgt
     2401 ttaacccaca ttccaactgg aattgttgtc caatcaacag tggatcgtac ccagtatgga
     2461 aatagagatc gtgccatgaa gatgttgcag gctaagctct atcaaatgga gcaagataag
     2521 aaggctgcgg aggtagattc tctcaaaggt gagaaaaagg agatcacttg gggaagccaa
     2581 atccgttctt atgtcttcac gccttatact atggtaaaag atcaccgaac tagctttgag
     2641 gttgctcagg tagataaggt tatggatggg gacctagatg gttttatcga tgcttatctc
     2701 aagtggcgaa ttagctaaga tagaaaggaa ctcacatgtc aattattgaa atgagagatg
     2761 tcgttaaaaa atacgacaac ggaacgactg ctctacgcgg tgtttcggtc agcgttcaac
     2821 cgggggaatt tgcttacatc gtaggacctt caggagcagg gaagtcaact tttattcgtt
     2881 ctctgtatcg tgaagtaaaa atcgataaag gaagtctatc agttgctggt tttaatctgg
     2941 ttaagatcaa aaagaaagat gtcccgcttc tacgtcgtag tgttggggtt gtcttccaag
     3001 attataaatt gttaccaaag aaaactgtct atgaaaatat tgcttacgct atggaagtaa
     3061 tcggggaaaa tcgccgtaat atcaaaagac gagtgatgga agttttggac ttggttggat
     3121 tgaagcataa ggttcgttct ttcccaaatg aactctcagg tggggagcaa cagcgaattg
     3181 cgattgcgcg tgcaattgta aataatccca aagtattgat agctgatgag ccaacaggaa
     3241 atctggatcc ggataattca tgggaaatta tgaatctctt ggaacggatt aacctacaag
     3301 ggacaactat tttgatggcg actcataata gccagattgt aaataccttg cgccaccgtg
     3361 tcattgccat tgaaaatggc cgtgtcgttc gtgacgaatc aaaaggagag tatggatacg
     3421 atgattagta gattttttcg ccatttattt gaagccttaa aaagtttgaa acgaaatggt
     3481 tggatgacag tagctgctgt cagttcagtc atgattactt tgaccttggt ggcaatattt
     3541 gcatctgtta ttttcaatac agcgaaacta gctacagata ttgaaaataa tgtccgtgta
     3601 gtagtttata tccgaaagga tgtggaagat aatagtcaga caattgaaaa agaaggtcaa
     3661 actgttacaa ataatgacta ccacaaggta tatgattctt tgaagaacat gtctacggtt
     3721 aaaagtgtta ccttttcaag taaagaagaa caatatgaaa aattaaccga gataatggga
     3781 gataactgga aaatctttga aggagatgcc aatcctctct atgatgccta tattgtagag
     3841 gcaaacactc caaatgatgt aaaaactata gccgaagatg ctaaaaaaat tgaaggtgtc
     3901 tctgaggttc aagatggcgg tgccaataca gaaagacttt tcaagttagc ttcatttatc
     3961 cgtgtttggg gactagggat tgctgctttg ttaattttta tcgcagtttt cttgatttca
     4021 aataccattc gtattaccat tatttcccgc agtcgcgaaa ttcaaatcat gcgcttggtc
     4081 ggagctaaaa acagttatat ccgtggaccg ttcttgttag aaggagcctt tatcggttta
     4141 ttgggagcta tcgcaccatc tgttttggtc tttattgttt atcaaattgt ttaccaatct
     4201 gtcaacaaat cgttggtagg gcaaaatcta tccatgatta gtccagattt atttagtccg
     4261 ttgatgattg ccctactatt tgtgattggg gttttcattg gttcattggg atcaggaata
     4321 tccatgcgcc gattcttgag gatttaggta aaatagctgc ttttatgagg agattgtaaa
     4381 atctcctttt ttgctacaag agtttttgaa aagagatgcg tagaagaaaa gagcttccaa
     4441 agaagtcccc cagagaagac ttcttgatgg ctaatttgaa cttgaaattt ggcgcactag
     4501 aaatttttta gaaaaaagtg taattttttt ataaaagcct ttacaaaaaa aattaaagtg
     4561 gtataattaa atcatagaag gtgcaaacgt tttcgtaaaa cgtttgccta aataaaaata
     4621 aaggagattt gaatgatgaa agatacattc aaaaatgtct tgtctttcga attttggcaa
     4681 aaattcggta aggctttgat ggtagttatc gcggttatgc cggctgctgg tttgatgatt
     4741 tcaatcggta agtctatcgt gatgattaac ccaaccttta caccacttgt catcacaggt
     4801 ggaattcttg agcaaatcgg ttggggggtt atcggtaacc ttcatatctt gttcgccctc
     4861 gctattggag gaagctgggc taaagaacgc gctggtggtg ctttcgccgc tggtcttgcc
     4921 ttcatcttga ttaaccgtat cactggtaca atctttggtg tatcaggcga tatgttgaaa
     4981 aatccagatg ctatggtaac tactctcttc ggtggatcaa tcaaagttgc tgattacttc
     5041 attagtgtta tggaagctcc agccttgaac atgggtgtgt tcgtagggat tatttctggt
     5101 tttattggag caactgctta caataaatac tataacttcc gtaagcttcc tgatgcactt
     5161 tcattcttta acggaaaacg ttttgtaccg tttgtagtta ttcttcgttc aataattgct
     5221 gcaattgtac ttgctgcttt ctggccagta gttcaaacag gtatcaatag ctttggtatc
     5281 tggattgcca actcacaaga aactgctcca atccttgcac cattcttgta tggtacattg
     5341 gaacgtttgc tcttgccatt tggtcttcac cacatgttga ctatcccaat gaactacaca
     5401 gctcttggtg gtacttatga ggtcttaact ggtgcagcaa aaggtagcca agtatttggt
     5461 caagatccac tttggcttgc atgggtaaca gaccttgtaa accttaaagg tactgatgct
     5521 agtcaatacc aacacttgtt agatactgtt cacccagctc gtttcaaagt tggacaaatg
     5581 attggttcat tcggtatctt gatgggtgtg gttgtggcta tctaccgtaa tgttgatgct
     5641 gacaagaaac ataaatacaa aggtatgatg attgcgacag ctcttgcaac attcttgaca
     5701 ggggttactg aaccaatcga atacatgttc atgttcgtcg caacacctat gtatcttgtt
     5761 tactcacttg ttcaaggtgc tgccttcgct atggctgacg tcgtaaacct acgtatgcac
     5821 tcattcggtt caatcgagtt cttgactcgt acacctattg caatcagtgc tggtatcggt
     5881 atggatatcg ttaacttcgt ttgggtaact gttctctttg ctgtaatcat gtactttatt
     5941 gcaaacttca tgattcaaaa attcaactac gcaactccag ggcgtaacgg aaactacgaa
     6001 actgctgaag gttcagaaga atctagcaac gaagtgaaag ttgcagcagg ctctcaagct
     6061 gtaaacatta ttaaccttct tggtggacgt gcaaacatcg ttgatgttga tgcatgtatg
     6121 actcgtcttc gtgtaactgt taaagatgca gataaagtag gaaatgcaga gcaatggaaa
     6181 gcagaaggag ctatgggtct tgtcatgaaa ggacaagggg ttcaagctat ctacggacca
     6241 aaagctgacg tattgaaatc tgatatccaa gatatccttg attcaggtga aattattcct
     6301 gaaactcttc caagccaaat gactgaagca caacaaaaca ctgttcactt caaaggtctt
     6361 actgaggaag tttattcagt agcagacggt caagttgttg ctttggaaca agtaaaagat
     6421 ccagtatttg ctcaaaaaat gatgggtgat ggatttgctg tagaacctgc aaatggaaac
     6481 attgtatctc cagtttcagg tactgtatca agcatcttcc caacaaaaca tgctcttggt
     6541 attgtgactg aagcaggtct tgaagtattg gttcacattg gtttggacac agtaagtctt
     6601 gaaggtaaac catttacagt tcatgttgct gaaggacaaa aagttgtagc aggtgatctc
     6661 cttgtcacag ctgacttgga tgctatccgt gcagcaggac gtgaaacttc aacagtagtt
     6721 gtcttcacaa atggtgatgc aattaaatca gttaaattag aaaaaacagg ttctcttgca
     6781 gctaaaacag cagttgctaa agtagaattg taatatactt gaggttggaa gctgtattcc
     6841 aacctcttat tttgggagaa aagaatgaaa tttttaacac tcaatactca cagttggatg
     6901 gagaaagaag cagaggaaaa attccagatt ttgcttgagg atattcttga aaaggactat
     6961 gatttgattt gtttccaaga aatcaatcag gagatcactt cgccagaggt ggaggttaat
     7021 gacctttatc aagctttgcc agcagctgag cttattcacc aagatcacta tgttagactc
     7081 ttggttgaaa agttgttaga gcaagggaaa aattactact ggacctgggc ttataaccat
     7141 atcggctatg accgctacca cgaaggtgtg gctatcttgt ctaaaacacc tattgaagcc
     7201 agagaaattt tggtttcaga tgtggatgat ccaacagact atcatactcg ccgtgttgcc
     7261 ctagctgaaa ctgtagtcga tggcaaggag ctagcagttg ccagtgttca tctctcttgg
     7321 tgggataaag gtttccaaga agaatgggca cgatttgagg ctgtcttgaa aaaattgaac
     7381 aagccacttt tactagctgg agattttaac aacccagcag gtcaggaagg ttaccaagct
     7441 attttagcta gtccattagg cttacaagac gcatttgaag ttgctcaaga gaaaagtggt
     7501 agctatactg ttccgcctga aattgatggc tggaaaggga acactgaacc ccttcgaatc
     7561 gattatgtct ttactaccaa agagttagcg gtggaaaatt tacatgtcgt atttgatggt
     7621 aacaagagtc cacaagtgag tgatcactat ggcttgaatg ctatgttaaa ctggaaataa
     7681 taactgaaaa gaggttggaa ctataaaatt ccagcctttt cttactagag aagctactgg
     7741 aaatagccta aataagtgag actactgtaa tggaataaaa tatggtataa ttgataagat
     7801 agatagaatc gaggatgtta tgtcatttac gaaatttcaa tttaaaaact atattagaga
     7861 agccttgaag gagttaaaat ttacaactcc aacagaggtg caagacaagt tgattcctat
     7921 tgttttggca ggtcgtgacc tagtaggaga atcaaaaaca ggttcaggta agactcatac
     7981 tttcttgtta ccgatttttc agcaattaga tgaagctagc gatagtgtac aagcagtgat
     8041 tactgcaccg agtcgtgagt tggctactca aatttaccaa gtagcgcgtc agatttcagc
     8101 tcactcagat gtcgaagttc gtgtggttaa ttatgtgggt ggtacggata aggctcgcca
     8161 gattgagaaa ttggcaagca atcagcctca tattgttatt ggaacaccag gccgtatcta
     8221 cgacttggtt aaatctggtg atttagctat tcataaagcc aagacatttg ttgttgatga
     8281 agcggatatg accttggata tgggattctt ggaaactgtt gataagattg ctggcagtct
     8341 tccaaaagac ttgcaattca tggtcttctc agcgactatc ccacaaaaac tgcaaccatt
     8401 cttgaaaaaa tacttatcca atcctgttat ggagaaaatt aagaccaaaa cggttatttc
     8461 tgacaccatt gataattggt tgatttcgac caagggacat gataagaatg ctcaaattta
     8521 ccagttgact cagttgatgc agccgtattt ggcaatgatt tttgttaaca ctaaaacgcg
     8581 tgctgatgaa ttgcattcat atctgactgc tcaaggcttg aaggttgcaa aaatccatgg
     8641 cgatattgcc cctcgtgaac gcaagcgaat catgaatcag gtgcaaaatc tggattttga
     8701 gtatattgtc gcaacagatt tagcagcgcg tgggattgac attgaaggtg tcagccatgt
     8761 catcaatgat gccattccgc aagacttatc tttttttgtt caccgtgttg gtcgtactgg
     8821 acgaaatggc ctaccaggta cagctattac cctttatcag ccaagtgatg actcggatat
     8881 ccgtgagttg gagaaattgg gaatcaagtt tagtcctaag atagtcaaag atggggaatt
     8941 tcaagatacc tatgaccgtg atcgtcgtgc caaccgtgag aaaaaacaag ataaacttga
     9001 tatcgaaatg attggtttgg ttaaaaagaa aaagaaaaaa gtcaaaccgg gttataagaa
     9061 gaaaattcaa tgggcggttg atgaaaagcg ccgtaaaacc aagcgtgctg aaaatcgcgc
     9121 tcgcggtcgt gcagagcgta aagctaaacg ccaaacattt taatagaaat tgttggagta
     9181 ctgagctcca acttttttat ttatgagaac gaactatcta aaccgaaaca ctacattaaa
     9241 gactgcaaat tgcgattaaa aatggtataa tgataaagtt atatagtccc gataagatgg
     9301 taggtattta ttacgaagag ttttcctatc agtagtttgc aattcgcaaa gaatgacggc
     9361 ttcgaggtcg ctcgtctttc tacggttgct gccatactgc ggtcacccta tgcgccttac
     9421 tagcttcaga aataaaaaag tatcgttttt atttctgaag ctagtaactc ttagataatg
     9481 gaaattccta tcagtacttt gtaactctat aacaatattt tttaaggggg gacattttta
     9541 tgtcagaacg taaattattc acgtctgaat ctgtatctga ggggcatccg gataagattg
     9601 cagaccaaat ttcagatgcg attttggatg ctattttagc aaaggatcca gaggcgcacg
     9661 ttgctgctga aacagctgta tatactggtt ctgtccacgt ttttggtgaa atttctacaa
     9721 atgcctatgt ggatattaac cgtgtggttc gtgataccat tgcagagatt ggttatacca
     9781 atacagaata tggattttct gctgagacgg tgggagtaca cccatctttg gtggaacaat
     9841 ctcctgacat cgctcaaggt gttaacgaag ccttggaggt tcgtggaaat gctgatcaag
     9901 atccactgga cttgattgga gcaggtgacc aagggctcat gtttggattt gcagtagatg
     9961 aaacagaaga gctcatgcca ttgccaattg cactcagtca taaattggtt cgtcgtctgg
    10021 cagaacttcg taagtctgga gaaattagct atctccgtcc agatgcaaaa tcacaagtta
    10081 cagttgagta cgatgaaaat gaccgtccgg tacgtgtaga tacagtcgtt atttctactc
    10141 agcatgatcc agaggccact aatgaacaaa tccatcaaga tgtgattgac aaggtcatca
    10201 aagaagttat tccatcttct tatcttgatg ataagacaaa attctttatc aatccgacag
    10261 gtcgttttgt aatcggtggt cctcaagggg actcaggttt gactggtcgt aagattattg
    10321 tagatactta tggtggctac tctcgtcatg gtggtggtgc cttctctggt aaagatgcga
    10381 ctaaggtgga tcgttcagcc tcttatgcgg ctcgctatat tgccaagaat atcgttgcag
    10441 cagaccttgc taagaaggca gaagtgcagt tggcctatgc tatcggtgtt gcgcaacctg
    10501 tttctgttcg tatcgatact ttcggtacag gaacagtagc tgaaagtcaa cttgaaaaag
    10561 cggctcgtca aatctttgac cttcgccctg cagggattat ccaaatgctg gaccttaagc
    10621 gtccaattta ccgtcaaaca tcggcttacg gtcacatggg acgtacagat attgatcttc
    10681 catgggaacg tttggataag gtagatgctt tgaaagaagc agtaaaataa gattttaaga
    10741 ggggaacgtc ctctcttttt tatagttttt aactgtactg ggatactgtt ctgaaaatcc
    10801 attttgagaa agtagagatt tacatgtata gtagattgaa actagaatag tacacctcaa
    10861 cttctaaaac attgttagaa atcgatttga ctgtcctgat cgatttctcc tgttcttgtt
    10921 tcattttact atatttcttt aaaaatgata aaggttgaga tttctcctcg taatagataa
    10981 tcatggggat atttcaagcc aaagttttat tcgttatcac ttgactattg caaggttttc
    11041 tagagcaaca gagtcatgga atggactcat ggttgagatt tctccttgtt gcttggactt
    11101 cattcaaaag tctgttaccc aagccttgtt caaacttcca atacactagc tgtttccata
    11161 gcatgacttc tgtactagac tttcttttcc gaataaatag atagaaccac agaatctagt
    11221 aaacctagaa ttaaaattat ggtataatat tagcaataaa agaaatctgg aggattagaa
    11281 tcatggtatc aacgaaaaca caaattgctg gttttgagtt tgacaattgc ttgatgaatg
    11341 cagcaggtgt ggcttgtatg acgatagagg agttagaaga ggtcaaaaac tcagcggcag
    11401 gaacctttgt tactaagaca gcgaccttgg acttccgtca ggggaatcct gagccacgct
    11461 accaagatgt tccacttggt tccatcaact ctatgggctt gccaaataat ggcttagact
    11521 attatttgga ttatctttta gatttgcagg aaaaagagtc gaaccgaact ttcttcttat
    11581 ctctggtcgg catgtctcca gaggaaaccc atactatttt gaaaaaagtc caagagagtg
    11641 attttcgtgg tctgactgag ctaaatcttt cctgtccaaa tgttccaggt aaacctcaga
    11701 ttgcctatga ttttgagaca acagaccgga ttttggcaga agtgtttgct tacttcacca
    11761 aacctcttgg aattaaattg ccaccttatt ttgatattgt tcactttgat caagcggcag
    11821 ctattttcaa caaatatccg ctcaagtttg tcaactgcgt taactctatc ggaaacggcc
    11881 tctatataga agatgaatct gtcgttattc ggcctaagaa tggttttggt ggaattggtg
    11941 gagaatacat caaaccgact gctctagcca atgttcacgc cttttatcaa cgtttaaatc
    12001 ctcaaatcca aattatcgga acaggtggcg ttctgactgg tcgagatgcc tttgaacaca
    12061 tcctctgtgg agcaagtatg gtgcaggtgg gaacgaccct tcacaaagaa ggcgtcagtg
    12121 cttttgaccg cattaccaat gaactgaaag caatcatggt ggaaaaaggc tacgagagct
    12181 tagaagattt ccgtgggaaa ttgcgctata ttgactaaat taaatcgaaa aatctgaaga
    12241 aaggagagac gatgctagcc attgaagaaa gtcagaagtt gactttatca aatttaccga
    12301 gcctgagcct atttacaggg acagatcagg gtcagtttga agtgatgaag agtcaagtgt
    12361 tgaaacagat tgggtatgat tctgctgacc tcaactttgc ctactttgat atgaaagaag
    12421 tagtttacaa ggatgtggaa ctggagttgg tcagccttcc tttctttgcg gatgagaaaa
    12481 tcgtgatatt agattatttt atggatatca cgactgctaa gaaacgcttt ttgacagatg
    12541 atgagcttaa gtcatttgag gaataccttg acaatccttc tccaacaacc aagttgataa
    12601 tctttgcaga aggaaagctg gatagcaaaa gacggttagt caaattactt aagcgtgatg
    12661 ccaaggtctt cgatgcagta gaagtaaaag aacaagaatt gcgccagtat ttccaaaagt
    12721 ggagtcagaa acaaggtctg cagtttacca atcattcttt tgaaaatctc ctcatcaagt
    12781 cggggtttca atttagcgaa atccagaaaa atcttctctt tttacagtcc tataaggcga
    12841 attctgttat cgaggaagag gatattgtta acgcaattcc caagaccttg caggacaata
    12901 tttttgattt aactcagttt attctgacta aaaagatgga tcaggcgcgc gatttggtga
    12961 gagacttgac cttgcaaggg gaagatgaaa tcaaactaat tgcagtcatg ctgggacaat
    13021 ttcggacttt tactcaggtg aagattttgg cggagtctgg ccaaacagaa tcgcagattg
    13081 caagtagttt aggtagttat ctgggacgta acccaaatcc ttatcaaatc aagtttgcat
    13141 taagagattc gagaggactt tctttgagct ttctgaagca agctatttcc tatttgattg
    13201 agacagacta tcagattaag acaggtcttt atgaaaaagg tttccttttt gaaaaggcac
    13261 tcttacagat tgctagtcag gtcaattgac atttgttgaa actacaaccc gcgtcaggat
    13321 tatcttgtcg tgggtttctt gctgatttgc ttatctataa gcaaaaaact tgaaaaaagt
    13381 gaatgtttgc gttatcattt tcatatagaa agaaaaagag gtattacaga tggctattat
    13441 cttaccagaa cttccatatg catacgacgc tttggaacca tacatcgatg cggaaacaat
    13501 gcatttgcac catgacaaac accatcaaac ttatgtcaac aatgccaatg cagctttaga
    13561 aaaacaccct gaaatcggtg aagaccttga agccttgctt gctgatgtag aatctatccc
    13621 agctgatatc cgtcaagcac ttatcaacaa tggtggcgga cacttgaacc acgctctttt
    13681 ctgggaattg atgactcccg agaaaacagc tccttcagca gaactagcag cagcaatcga
    13741 tgcaacattt ggttcatttg aagaatttca ag
//
LOCUS       DAVAAL010000042        13051 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVAAL010000042 DAVAAL010000000
VERSION     DAVAAL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.043"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            61..924
                     /locus_tag="VQM60_001049"
     CDS             61..924
                     /locus_tag="VQM60_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1080926.1"
                     /translation="MNTLAEKFRLKRKELGLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDNLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLMELYQTKNFEHQEFLFGYIRVRYNYSHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDKEQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1230..1850
                     /locus_tag="VQM60_001050"
     CDS             1230..1850
                     /locus_tag="VQM60_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080927.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYTGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1936..2379
                     /gene="tsaE"
                     /locus_tag="VQM60_001051"
     CDS             1936..2379
                     /gene="tsaE"
                     /locus_tag="VQM60_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET1080928.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILIGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2369..2887
                     /locus_tag="VQM60_001052"
     CDS             2369..2887
                     /locus_tag="VQM60_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1080929.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLID"
     gene            2895..3911
                     /locus_tag="VQM60_001053"
     CDS             2895..3911
                     /locus_tag="VQM60_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET1080930.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            3996..5252
                     /locus_tag="VQM60_001054"
     CDS             3996..5252
                     /locus_tag="VQM60_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET1080931.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGNKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5307..6473
                     /gene="recA"
                     /locus_tag="VQM60_001055"
     CDS             5307..6473
                     /gene="recA"
                     /locus_tag="VQM60_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET1080932.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            6780..8150
                     /locus_tag="VQM60_001056"
     CDS             6780..8150
                     /locus_tag="VQM60_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET1080933.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8526..9482
                     /gene="lytA"
                     /locus_tag="VQM60_001057"
     CDS             8526..9482
                     /gene="lytA"
                     /locus_tag="VQM60_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET1080934.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            9841..10002
                     /locus_tag="VQM60_001058"
     CDS             9841..10002
                     /locus_tag="VQM60_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1080935.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            9989..10195
                     /locus_tag="VQM60_001059"
     CDS             9989..10195
                     /locus_tag="VQM60_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080936.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            10241..10600
                     /locus_tag="VQM60_001060"
     CDS             10241..10600
                     /locus_tag="VQM60_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080937.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKETVLG"
     gene            10729..11154
                     /locus_tag="VQM60_001061"
     CDS             10729..11154
                     /locus_tag="VQM60_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080938.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKV"
     gene            11562..12356
                     /locus_tag="VQM60_001062"
     CDS             11562..12356
                     /locus_tag="VQM60_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080939.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQYVLK"
     gene            12360..12557
                     /locus_tag="VQM60_001063"
     CDS             12360..12557
                     /locus_tag="VQM60_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080940.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            12547..>13051
                     /locus_tag="VQM60_001064"
     CDS             12547..>13051
                     /locus_tag="VQM60_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080941.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNLEFPSMIELFSIL
                     PEYPLKKDGREISLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCGLYSSTI
                     SSNHVSFH"
BASE COUNT         4153 a         2081 c         2821 g         3996 t
ORIGIN      
        1 ctattttgaa ctaaaaattt tataatgaaa ttaagcaaat aggaaggttt attatcttta
       61 atgaatacac tcgcagagaa attcagatta aaaagaaaag agctaggact ctcccaacaa
      121 actcttgcag aaggaatttg tgaacaaagc cagattagta aaattgagag agggcatttc
      181 attccctccg cagacctttt gttcaaactc tcacaacgac ttgaagtacc attagattat
      241 ttttttaatg aacaaattga aattaaatct aacctctcta atttcaagca attatctgct
      301 cgactattag atgacagaaa ttatgacaat ttggaatata tttatagaat agagattgaa
      361 cgaagtactt ttctaacact agaagaccga acttaccttg aatggattaa agctattatt
      421 gacttctatc aatatgacag taagtgtgag gctatttctt cattggaaaa tatattatta
      481 aaagtctcct caaatactct gatttattta aaggcattga atactctatc taatttctat
      541 tccttagtgg gtcgtgaaca agaatatgag gcaaactact ctcatttaat ggagttatat
      601 cagacaaaaa attttgagca tcaagagttt ttatttggct acatcagagt tcgttacaac
      661 tactctcact acctagtgtc aaaggaaaaa tataacgaag ctatccaaga agctcttgag
      721 acgattgaac tctgtaaaca aagacagaca agctaccaac tggctcccct acttattctt
      781 gtaggaaatg ctggagccaa atttctagac aaagaacaag tcaaaaatta ttatatagaa
      841 gcaagagagt tatgtaagat ttataacaat cctttaatgt tgatgaagat agaaaattat
      901 ttgaaggaat tagatactgt atagttaatc ttgacattat agtttttctg aaacgttaaa
      961 taacctgtaa ggctgatagt gaaatgaata ctatcagcct tatttcaagt ttatattctt
     1021 ccaaaatctc ttcaaaccac gtcagctcta tctgcaacct caaaacagtg ttttgaacaa
     1081 cctgcggcta gcttcctagt ttgctctttg attttcattg agtattagcc tctcgtgttt
     1141 gtttagcgtc ttaaaaagac atttgggaag attggaaaca gttctctctt ttctccgagt
     1201 ataatgattg gtaggaaggg agagagaaga tgaaatcaat gagaatctta tttttgttag
     1261 ctttaattca aatcagtttg agtagctgtt tcctatggaa ggaatgcatc ttgtccttta
     1321 aacaaagtac agcttttttc atcggaagca tggttttcgt ttcaggaatc tgtgctggag
     1381 taaattatct ttatacccgt aagcaagaag tccatagtgt cctagccagt aagaagtcgg
     1441 tgaagctttt ttacagtatg ttactcttaa ttaatttgtt aggagctgtt cttgttttgt
     1501 cagataactt gttcatcaaa aatacgctgc agcaagaatt agttgacttt ttattgccat
     1561 ccttcttttt cctatttggg ctagatttgc tgattttttt acccttgaaa aaatacgtgc
     1621 gcgattttct tgctatgcta gacagaaaaa agacagtgtt ggtgactatt ttagcaacac
     1681 ttcttttctt aagaaatcca atgaccattg tctcacttct gatttatact ggactgggct
     1741 tgttttttgc agcctatctt gtcccaaatt cggttaagaa ggaagtttcc ttttatggtc
     1801 atattttccg agatcttgta ttggtcattg ttacgctcat tttcttttag agaaagcctg
     1861 ttttttatgg atgttatgat gataagttta gtaggaaaat agacacaaaa gaaaagtttt
     1921 tggtataata agagtatgta cacaaaaaat gaagaagagt tgcaagcctt aggggagcgt
     1981 ttgggccatc tattagcaaa gaatgatgtt ttaatcttaa ttggagaact gggtgcaggt
     2041 aaaacgacct ttactaaagg acttgcaaaa ggattacaga tttctcaaat gattaaaagt
     2101 cctacctata ctatcgtgag agagtatgaa ggtcgacttc cactttatca cctagatgtt
     2161 tatcgtattg aaggagatgc tgattctatc gacttggatg agtttatctt tggtggcggc
     2221 gtgactgtta ttgagtgggg aaatctctta ggagatgcct tgccagatgc ttatttagaa
     2281 ttggaaattc taaaagaagc agatggacgc cgtttaaatt ttcaggcaaa gggtttgcgt
     2341 gctgagaaat tgttagagga gcttcaatat ggagtatgaa ttgctcatta gggaagcaga
     2401 gcccaaagat gcagctgaat tagtggcctt tttaaatcgt gtgagtttgg agacagactt
     2461 taccagccta gacggagatg gtattctctt gactagtgag gagatggaaa tattcctcaa
     2521 caagcaagct agttcggaca atcagataac cttacttgca ttcttaaatg gcaaaattgc
     2581 tggtattgta aatattacag ctgaccagcg taagagagtc cgtcatattg gagatctctt
     2641 cattgtgatt ggaaaaagat attggaataa tggcttggga agtttgttgc tagaagaagc
     2701 gatagagtgg gcacaagcaa gtggcattct gcgtcgtctc caactgactg tccaaactcg
     2761 taatcaagca gcagtccatc tttatcaaaa gcatggcttt gtcattgaag gtagccaaga
     2821 gcgtggggca tatatagaag aagggaaatt tatcgatgtt tacttgatgg gtaaactgat
     2881 agattagtag aaatatggtt aaaaaaatta ttggaatggt gctagcttta ctttctgtaa
     2941 ctgtagtagg agtaggtgtt tttgcttata ctatttatca acaagggaca gaaaccttag
     3001 ctaaaaccta taaaaaaatc ggcgaagaaa ccaaggttat tgaagcgact gaacctctaa
     3061 ccattctgtt aatgggagtg gacaccggaa atgctgaacg aactgaaact tgggtcggta
     3121 gaagtgatag catgatcttg atgacagtga atcctaaaac gaaaaaaaca acaatgatga
     3181 gtttagagcg ggatattctg acgcgcattg aatcagggaa tggtcaggct catgaagcga
     3241 aactgaactc agcatatgca gatggtggag cagagcttgc tatagaaacc attcaaaaaa
     3301 tgatgaatat ccatattgat cgctatgtga tggtcaatat gagagggttg caaaaactag
     3361 tggatgcagt aggaggtatt acagtcaata atatcctagg tttcccaatt tctatcagtg
     3421 accaagaaga atttaatact atttctatcg gtgttgggga gcaacatatt gggggagaag
     3481 aagccctagt ctatgcacga atgcgttacc aagatcctga gggggattat ggtcgtcaaa
     3541 aacgtcaacg tgaagttatt caaaaagtca tggaaaaagc tctcagttta aatagcgttg
     3601 gtcattatca agagattcta aaagctttga gtgacaatat gcagaccaat attgatttgt
     3661 ctgcaaaaag tatccctaac ttgctaggct ataaagattc atttaaaacc attgaaactc
     3721 agcagttgca gggtgaagga gagatacttc aaggtgtttc ttaccagatt gtttcgagag
     3781 cacatatgtt ggaaatgcaa aatatactcc gacgttcttt gggacaagaa gaagttactc
     3841 agcttgaaac caatgcggtt ttatttgaag atttatttgg cagagcacct gttggtgatg
     3901 aagataatta acttttgata caaataaaaa aatcaatcgt aggaaaattc ctgcgatttt
     3961 ttcaaaaaaa tacgaataga taggtaggag gaaacatgaa agcagaaatc attgctgttg
     4021 gaacagagat tttgacagga cagattgtca acaccaatgc ccagtttttg tcggaaaaac
     4081 tagctgagat tggggtagat gtatattttc agacggctgt aggagacaat gaagttcgtc
     4141 tcttgtcttt gcttgagatt gccagtcaac gtagcagtct ggtgattttg acaggcggtt
     4201 tggggccaac tgaggacgac ctaaccaaac aaaccctagc taaattttta gggaaagcat
     4261 tagtctttga tcctcaggct caggagaagt tggatatctt ttttgccctg cgaccagact
     4321 atgcccgaac accgaataac gaaagacaag ctcaaattgt agaaggagcg attccactgc
     4381 caaacgaaac aggactggct gttggaggaa aattagaagt agacggagtg acctatgtcg
     4441 tccttccagg tccgccaagt gaattgaaac ccatggtctt aaaccaactt ctacccaagt
     4501 tgatgacagg gaacaagctg tattcccgag ttcttcgttt ctttgggatt ggcgagagcc
     4561 agttggttac gattttggct gatttaattg ataatcagat cgatcctacc ttggcccctt
     4621 atgccaagac aggagaagtc actctacgtc tgtcaacaaa ggctagcagt caagaagagg
     4681 cgaatcaagc gctggatatc ttggaaaatc aaatcttgga ctgccagact ttcgaaggaa
     4741 tttctttacg agacttttgc tatggttatg gggaagaaac tagtttagcc agcattgtgg
     4801 tagaagaact gaaaaggcaa gggaaaacca tcgcggctgc agagagtttg acggcaggtc
     4861 ttttccaagc taccgtggcg aatttttctg gagtttcaag tatatttgag ggtggttttg
     4921 tgacctatag cttggaggaa aaatcaagga tgttggatat tcctgccaag aatttggaag
     4981 aacatggtgt ggtgtctgaa tttacagctc agaagatggc tgagcaggca cgaagcaaga
     5041 cccagtctga ttttggaatt agtttgactg gagtggcagg accagatagc ctagaaggac
     5101 acccagttgg gacagtcttc ataggcttgg cgcaagatca aggaactgag gttatcaagg
     5161 ttaatattgg aggcagaagc cgagcagatg tacgtcacat tgcggttatg catgccttta
     5221 acctagttcg caaggcttta ttaagtgact aacttttgat ataatagtag ataggtctga
     5281 ggatcattag aatgtaggag aatagaatgg cgaaaaaacc aaaaaaatta gaagaaattt
     5341 caaaaaaatt tggggcagaa cgtgaaaagg ccttgaatga cgctcttaaa ttgattgaga
     5401 aagactttgg taaaggatca atcatgcgtt tgggtgaacg tgcggagcaa aaggtgcaag
     5461 tgatgagctc aggttcttta gctcttgaca ttgcccttgg ctcaggtggt tatcctaagg
     5521 gacgtatcat cgaaatctat ggcccagagt catctggtaa gacaacggtt gcccttcatg
     5581 cagttgcaca agcgcaaaaa gaaggtggga ttgctgcctt tatcgatgcg gaacatgccc
     5641 ttgatccagc ttatgctgcg gcccttggtg tcaatattga cgaattgctc ttgtctcaac
     5701 cagactcagg agagcaaggt cttgagattg cgggaaaatt gattgactca ggtgcagttg
     5761 atcttgtcgt agtcgactca gttgctgccc ttgttcctcg tgcggaaatt gatggagata
     5821 tcggagatag ccatgttggt ttgcaggctc gtatgatgag ccaggccatg cgtaaacttg
     5881 gcgcctctat caataaaacc aaaacaattg ccatttttat caaccaattg cgtgaaaaag
     5941 ttggagtgat gtttggaaat ccagaaacaa caccgggcgg acgtgctttg aaattctatg
     6001 cttcagtccg cttggatgtt cgtggtaata cacaaattaa gggaactggt gatcaaaaag
     6061 aaaccaatgt cggtaaagaa actaagatta aggttgtaaa aaataaggta gctccaccgt
     6121 ttaaggaagc cgtagttgaa attatgtacg gagaaggaat ttctaagact ggtgagcttt
     6181 tgaagattgc aagcgatttg gatattatca aaaaagcagg ggcttggtat tcttacaaag
     6241 atgaaaaaat tgggcaaggt tctgagaatg ctaagaaata cttggcagag cacccagaaa
     6301 tctttgatga aattgataag caagtccgtt ctaaatttgg cttgattgat ggagaagaag
     6361 tttcagaaca agatactgaa aacaaaaaag atgagccaaa gaaagaagaa gcagtgaatg
     6421 aagaagttcc gcttgactta ggcgatgaac ttgaaatcga aattgaagaa taagctgtta
     6481 aagcagtgga gaaatccgct actttttcga tttttgattc aagtttttag attatatata
     6541 gtagcttgaa ataagatatg aacaacttta ttaggaaagt caaattaatt tctagaaatg
     6601 ttttagcagc tacagcgtac tattccaaac tcaaccaact ataatagatc gaaactagaa
     6661 tagtacacat ctacttctaa aacattgtta aaaatcgatt tgactgtcct tatttcattc
     6721 cgctatatat aagtttgctg tttcttgtcg ctcctctgga aagctgatat aatagcttta
     6781 tgaataaaaa acgaacagtg gacctgatac atggtccgat tcttccctcg ctcttaagct
     6841 tcacctttcc aatcttgcta tcaaatattt ttcaacagct ctataacact gctgatgtct
     6901 tgattgttgg acgatttctt ggtcaagaat ccttggctgc agtaggagcg acgacagcga
     6961 tttttgacct gattgtaggc tttacacttg gtgttggcaa tggcatgggg attgtcattg
     7021 ctcgttatta tggggctcgg aatttcacta aaatcaagga agcagtagca gccacctgga
     7081 ttttaggtgc tcttttgagc attctagtta tgttgctggg ctttcttggc ttgtatcctc
     7141 tcttgcaata cttagatact cctgcagaaa ttcttcctca atcttatcaa tatatttcta
     7201 tgattgtgac ctgtgtaggt gtcagctttg cttataatct ttttgcaggc ttgttgcggt
     7261 ctattggtga cagtctagca gccctgggat ttctgatttt ctctgccttg gttaatgtgg
     7321 ttctggatct ctattttatt acgcaattgc atctgggagt tcaatccgca ggacttgcta
     7381 ccattatttc gcaaggttta tcagcggttc tctgctttta ttatattcgt aaaagtgtgc
     7441 cagaactctt gccacagttt aaacatttca aatgggacaa aagcttgtac gcggatctct
     7501 tggagcaagg tttggctatg ggcttgatga gttcaattgt atctatcggc agtgtgattt
     7561 tacagttttc tgttaataca tttggtgcag tgattattag tgcccagacg gcagctcgac
     7621 gcattatgac ctttgccctt cttcctatga ccgctatttc tgcatcaatg acgacctttg
     7681 cttctcagaa tctaggagct aagcgacctg accgtattgt tcaaggtctt cgaatcggca
     7741 gtcgtttaag tatatcctgg gcagtttttg tttgtatttt cctctttttt gccagtccag
     7801 ctttggtttc cttcttggct agttcgacag atggttactt gatagaaaat ggaagtctct
     7861 atctgcaaat cagttcaacc ttttatccca ttttgagcct cttgttgatt tatcgcaatt
     7921 gcttgcaggg cttggggcaa aagatccttc ctctagtttc tagctttatt gaactaatcg
     7981 gaaaaatcgt ttttgtggtt ttgattattc cttgggcagg atataagggt gttatccttt
     8041 gtgaacctct tatctgggtt gccatgacag ttcaactgta cttctcatta ttccgtcatc
     8101 ccttgataaa agaaggcaag gcaatcttgg caaccaaagt gcaatcctag ttggatttac
     8161 tgaataaaat ccatttcctc tagtgaaaat cgaaaaaact tgtgttctcg tctttagttt
     8221 ggtgttgaaa atagtttaac agacttttga cttcttttat atgatataat aaagtatagt
     8281 atttatgaaa aggacatata gagactgtaa aaatatactt ttgaaaagct ttttagtctg
     8341 gggtgttatt gtagatagaa tgcagacctt gtcagtccta tttacagtgt caaaatagtg
     8401 cgttttgaag ttctatctac aagcctaatc gtgactaaga ttgtcttctt tgtaaggtag
     8461 aaataaagga gtttctggtt ctggattgta aaaaatgagt tgttttaatt gataaggagt
     8521 agaatatgga aattaatgtg agtaaattaa gaacagattt gcctcaagtc ggcgtgcaac
     8581 catataggca agtacacgca cactcaactg ggaatccgca ttcaaccgta cagaatgaag
     8641 cggattatca ctggcggaaa gacccagaat taggtttttt ctcgcacatt gttgggaacg
     8701 gttgcatcat gcaggtagga cctgttgata atggtgcctg ggacgttggg ggcggttgga
     8761 atgctgagac ctatgcagcg gttgaactga ttgaaagcca ttcaactaaa gaagagttca
     8821 tgacggacta ccgcctttat atcgaactct tacgcaatct agcagatgaa gcaggtttgc
     8881 cgaaaacgct tgatacaggg agtttagctg gaattaaaac gcacgagtat tgcacgaata
     8941 accaaccaaa caaccactca gaccatgtgg atccataccc ttacttggca aaatggggca
     9001 ttagccgtga gcagtttaag tatgatattg agaacggctt gacgattgaa acaggctggc
     9061 agaagaatga cactggctac tggtacgtac attcagacgg ctcttatcca aaagacaagt
     9121 ttgagaaaat caatggcact tggtactact ttgacagttc aggctatatg cttgcagacc
     9181 gctggaggaa gcacacagac ggcaactggt actggttcga caactcaggc gaaatggcta
     9241 caggctggaa gaaaatcgct gataagtggt actatttcaa cgaagaaggt gccatgaaga
     9301 caggctgggt caagtacaag gacacttggt actacttaga cgctaaagaa ggcgccatgg
     9361 tatcaaatgc ctttatccag tcagcggacg gaacaggctg gtactacctc aaaccagacg
     9421 gaacactggc agacaagcca gaattcacag tagagccaga tggcttgatt acagtaaaat
     9481 aataatggaa tgtctttcaa atcagaacag cgcatattat taggtcttga aaaagcttaa
     9541 tagtatgcgt ttttttgtgg agatatttcc ttcaatgttt gctactatat taaacaaaaa
     9601 tcagaaagca aactagaaag ttatgctcaa ataaaatcta aatttgacaa tgtaaaccga
     9661 gtcggatagc tttaagtact gttttgaggt tgaagatacg atttttgata ggaactcatc
     9721 aattttagat ttttaagcag catcaataaa ttgcttcctt gttttgtcat aattttttta
     9781 tttaaaaaat tatgacaaga gtgtgctatt ctttttatga gaggtgtatg aatatgataa
     9841 atgtatgtga taaatgtatg tgatgttgga aaaagaataa aagaacttag aatatcttca
     9901 aatcttactc aagataagat tgctgagtat ttgtctttga atcaaagcat gattgccaaa
     9961 atggaaaaag gtgaaaggaa tatcacgaat ggatttaagt aataaagctc caaatcttag
    10021 aaaaaagttg ggagctgatg gtgaatcgcc gatagatatt tttaaattgg tacaaaagat
    10081 agaaaatttg acgctggtat tttatggact cggaaagaat attagcggag tctgttataa
    10141 aggaactcag ttcagtctca ttgcagtcaa ttcagacatg tcattaggaa ggtaaagatt
    10201 ttctttagca catgaactgt atcatcttta ttatgatgag gtgaagaaga gttcagtcag
    10261 tcttatcttg attggtgaag gagatgaaac tgaaagaaaa gcggatcagt ttgcttctta
    10321 ttttttaatt ttcccatctt cactgtatag gatggttgag gaaattagag aaaatgccaa
    10381 tagaactcat cttgaagtag aagatattat aaaattgggt cagttttatg gtatcagtca
    10441 taaagctatg ttatatagat tgagaaatga tggatacctt gatgcagaag aaattaaaaa
    10501 tatggatatt agtgttatag agacagcttc aagattaggc tatgatacaa gtttatatcg
    10561 tcctttgtca gaaagtaaaa aggaaacggt attaggataa tatattaatt cgactgaaca
    10621 acttttagaa aataacagaa tttcgcaagg gaagtatgag gaactgttac tagatgcttt
    10681 cagatatgat attgtatatg ggctatatga agaggaggga gttgtcgttt gactagtcgt
    10741 gtatttattg atgcagattg tatttcagta tttttatggg ttggcactga acatctttta
    10801 gaaaagctct attcgggtaa aattgttatt ccacaagagg tgtatgatga aatcaatata
    10861 cctacaattc cccatttaaa atctaggata gaccagttgg tagctaaggg ttcagctgag
    10921 attgtgagca tagacattgg aactgaagaa tacgcattat atagagattt aacaagaaat
    10981 catgatagta acaagattat tggtaaggga gaagcggctt ctatttcctt agcgaaaaag
    11041 cataatggga tattaggaag taataaccta agagatgtta aatcatatgt agaagaattt
    11101 tctttagaac atatgacaac aggagatata ctgattgaag cgtttaaagt gtaatttatt
    11161 actgaacaag agggcaatca tatctggaat aatatgctta aaaagagaag gaaaattggt
    11221 gcaaattcat tttcagacta tcttcgtgga agtattcatc aaaatagaca aaaataaatt
    11281 tggataaatc gaactcacta ttcaggaggc atatgagcaa ttcgaaaaag aaaagtgtca
    11341 aattgagcct ataggagtag aagtgaaata gtaagtcctg catagtggat gagagaaaag
    11401 ttctccttga agttttcctg aactatcagt cgcatgtcaa acgatatgta gggtaatgtg
    11461 agaggggata gcgagtagtt tttggttatt ttatcaaaaa acttatattt tattatactg
    11521 aatgataaaa tataataaaa atgatagaat aaggaaaaaa catgaatgtc aaaaagataa
    11581 tgtcaatttt tcaatccttt tatgttgatg tcagtattga ggaactgact ttgactttac
    11641 caatcagttt tgtaaaaagg tttgagtata ctcaaatgac ttttcataag gaatcatttt
    11701 tattgattaa agaaaagaga agggggagtt tgagttcatt tgttactcag gctcgcacta
    11761 tgggtgaaaa agccaatatg gatgttgttt tggtgttttc gaagttatca gacagtgaaa
    11821 aaaagcaatt acttcaagct agagttccgt ttgtagactt taagggaaac ctcttcttcc
    11881 ctccattggg actagtactg aatgcgaatg atactgaagt ccctaaggaa ttaacaccta
    11941 gcgaacaatt aacgtggatt gcctttttat tgacaaaagg tcaaaaagta gtagatgttg
    12001 atttgctttc acaagtcact ggacttccaa actcaacaat ttataggtgt ttgaggactt
    12061 ttaaagcttt atattggtta aacaagcaaa ataagcttta cacatatacg gtgtcaaaga
    12121 aagaattatt cttaaaatcc gtgtcatgtt tatttaatcc catcaaaaaa cggattttat
    12181 tgccagatgg cgatataaag cagataaaat ctgtttctaa ccttctatat ggtggtgctt
    12241 atgctttgtc gcattcaact tttttagctg aaacggatga aaatactagc tatgtcatat
    12301 ggcagagaaa attcaatcag ttatccttgc cactttctca gtatgtttta aaatgaaaga
    12361 tgctagagat atggaaatat cgtccttttg tatctgagtt ttggaatgat tttaaaaata
    12421 atcatgataa acaatttgta gatccgattt ctctttattt gaccttaaaa gatgatgatg
    12481 acccacgtat agaggaagag agtgaagcac tagaaaatat gatattacag tatctgggag
    12541 aagatgatgc cagctaatac gaaagttatt tttcaagaaa tgtttgcgga ttttcagaac
    12601 tattatgttc tgattggggg aactgctacc tctatcgtat tggattcgca aggatttaaa
    12661 agtcgcacaa caaaagatta tgatatggtc atcattgatg aagtaaaaaa taaggaattt
    12721 tatactacct tgaatcattt tttagaattg ggagagtatc aaggaagtca gaaagatgag
    12781 aaagcgcagc tttttcgatt tacaacaact aatcttgagt ttccttctat gattgaacta
    12841 tttagtatct taccagaata tccattaaag aaggacggtc gagaaatttc cttatatttt
    12901 gaccaagatg ctagtttatc agccttatta ttggatgaag attattataa tatattggtg
    12961 catgtaaaag aaaccattca ggggtattcg gtattgagta attgtggtct atactcttcg
    13021 acaatctctt caaaccacgt cagcttccat c
//
LOCUS       DAVAAL010000043        12864 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVAAL010000043 DAVAAL010000000
VERSION     DAVAAL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.044"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(58..1299)
                     /locus_tag="VQM60_001065"
     CDS             complement(58..1299)
                     /locus_tag="VQM60_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET1080942.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAISAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            1402..2511
                     /locus_tag="VQM60_001066"
     CDS             1402..2511
                     /locus_tag="VQM60_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET1080943.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            3157..3504
                     /gene="ssrA"
                     /locus_tag="VQM60_001067"
     tmRNA           3157..3504
                     /gene="ssrA"
                     /locus_tag="VQM60_001067"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3697..3784)
                     /locus_tag="VQM60_001068"
     tRNA            complement(3697..3784)
                     /locus_tag="VQM60_001068"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3748..3750),aa:Ser,seq:gga)
     gene            3947..4687
                     /locus_tag="VQM60_001069"
     CDS             3947..4687
                     /locus_tag="VQM60_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET1080944.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPYSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGINGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            4684..5595
                     /gene="pfkB"
                     /locus_tag="VQM60_001070"
     CDS             4684..5595
                     /gene="pfkB"
                     /locus_tag="VQM60_001070"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET1080945.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            5592..7544
                     /locus_tag="VQM60_001071"
     CDS             5592..7544
                     /locus_tag="VQM60_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET1080946.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            7708..9933
                     /locus_tag="VQM60_001072"
     CDS             7708..9933
                     /locus_tag="VQM60_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET1080947.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQTELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(9977..10405)
                     /locus_tag="VQM60_001073"
     CDS             complement(9977..10405)
                     /locus_tag="VQM60_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080948.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENKSNQ"
     gene            10543..11685
                     /locus_tag="VQM60_001074"
     CDS             10543..11685
                     /locus_tag="VQM60_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET1080949.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLVGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            11694..>12864
                     /gene="thiI"
                     /locus_tag="VQM60_001075"
     CDS             11694..>12864
                     /gene="thiI"
                     /locus_tag="VQM60_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET1080950.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQ"
BASE COUNT         3720 a         2382 c         2816 g         3946 t
ORIGIN      
        1 tctttgattt tgaagatgaa cttgtatcaa aaaatcgcgg tttaaaatcg cgatttttta
       61 taatttctcg ttaacaaaac ggacaaactg attccaccaa acttttaaga agaaggcttt
      121 ttcaattttc ttgtctgcta ccatttcgaa actagggcgc tctgtggtga tgtaaccttg
      181 accaatcaag tccttgtctt cataagtcaa atggccaacc actgttccag cttcaagcgg
      241 tgctgagatt gctttggaat caggtgtgaa ttgaacagat tgggaagatt gattcccaac
      301 acgttcgatt agatagatat cctctggagc cactgcagtt actgtatctt cttttccatc
      361 ttgtacaggg gctttgctat cttgataggc atcgccttgt tgaacgattt tgcgaagtgt
      421 aaatgtagaa gaaatataat ccattaggga agatgtagct gtaaatcgag cgtaaggatt
      481 attgtcttga tgatctgcat ttaaaacaac tgtgatgact ctcatgcctt tttcgacagt
      541 agtaccaaca aaagactctc cagccttatc tgttgttcct gtttttagcc catcaaaacc
      601 accacggtaa gcaggcatac cttctaacat gtagttggtt gaagtgattg tcattccagc
      661 aaaagtagaa gaaggttttt tggtgatttc caagacttgt gggtattttt tgatgaggtt
      721 gcgagcaaca atagcgacat cataagcact aagcttattt tcctcatctt ttttagaacc
      781 tgggtaaatg ttatccccta gagtttcatt gttaagacct gtcgtattga caacagtggc
      841 atcctgaatt ccccattcca agagttttgc ccgcatcata tcgacgaaat ctttttctga
      901 gccagcaatt ttctcagcta gggcaatagc ggcgctgttg gcactagata ccagagttgc
      961 ttcaagcaac tcttcgacag tataattacg ggcctccata ggaatattac tggcttcaga
     1021 atttgtcgtc aattgataag gataatcaga aatatctaca ggagtggaga gggtaatact
     1081 tccgttttcc aaagcttcat agaccagata aacagtaatc aattttgtta tggaagcaat
     1141 ttcgacaggt tgcgttgcat ccttctcata gagaatttta ccagtatttg cctcaacagc
     1201 aatcgcatgt ttagcggcaa tggtaaaatc ttgagcaaca gcagtagaag caccccctaa
     1261 aagagagaca gttaacaaag ttaaaaatat ttttttcata gtagtcttat tctatcataa
     1321 agaaaaaaaa tattcttgct ttaataattc atctgttaag ctttttgaaa atatggtaaa
     1381 ataaagtgag ggaggtaact catgtttcgt agaaataaat tatttttttg gaccacagaa
     1441 attttactct taaccatcat cttttaccta tggagacaga tgggatcttt gattaaccct
     1501 tttgttagcg tgcttaatac aattatgatt ccatttttat tagggggctt tctttattat
     1561 ttgacaaacc ctattgttac tttcttaaat aaagtctgta aactcaatcg tttgcttggt
     1621 attttaatta ccttgtgtac tttggtctgg ggaatggtca taggtgttgt ctatctctta
     1681 cctattttga ttaatcagtt atctagtttg attatatcta gtcaaactat ttatagtcga
     1741 gtacaagact taatcataga cttatctaat tatcctgcgc tccagaattt ggatgtagaa
     1801 gctacaattc agcagttaaa cttatcctat gttgatattc ttcaaaatat cctaaatagc
     1861 gtatcaaata gtgtggggag cgtcttgtca gctcttatca gtactgtttt gattttgatt
     1921 atgactccag tttttttggt ttatttctta ttagatggac ataaattctt gcccatgctt
     1981 gaaagaacga ttctaaagag ggatcgcttg catattgcag gcttattaaa gaatttaaat
     2041 gcgacgattg ctcgctatat tagtggagtt tcgattgacg caatcattat aggttgttta
     2101 gcttatattg gctatagtat tattggttta aaatatgctt tagtttttgc cattttttct
     2161 ggtgtagcca atttaattcc ttatgtgggg ccaagtattg gtttgattcc tatgatcatc
     2221 gcaaatatat tcactgatcc ccatagactg ctgattgcag tgatttatat gcttgttgtt
     2281 cagcaggtag atggcaatat cttatatcct cgaatcgtag gaagtgttat gaaggttcat
     2341 ccaatcacga ttttagtttt acttttgttg tcaagcaata tctatggtgt agttggaatg
     2401 attgtcgcag tgccaaccta ttctatcttg aaagaaattt ctaagttctt atcccgtttg
     2461 tatgaaaatc ataaaataat gaaagaacga gaaagagaat tagctaagta aaagtcagga
     2521 gaaccctgat ttttctttac tggaagtggc ctttagatta gaagactgaa aataagttaa
     2581 agtcttaaac taattttcac agctaagaat agtagaagtt aatctgataa aaatcgaaaa
     2641 aaccagtgga attccgtgtc agggtaagtt ccactggttt tcatagtcta ttaaagttcg
     2701 aatgaaacct atttatagta tattgaaact agaatagtac acctctaatt ctaaaacatt
     2761 gttagaaatc gatttgactg tcctgatcta ttcgtcctat tcttatttca ttttactata
     2821 ttttggtgca ataagtgaaa agtagtccga ataatataag gattgatttt atagttttta
     2881 aactcaaatg aattaaaata aagagagtac gaaaattctc atctgaaagt attttagaat
     2941 aattctcttc gtgaatttct tcaaaacaga tagcttcatc ttaggtatgt gttaattgga
     3001 tttcgtaagc tctatttaca atcgtaaagt tatgcactga gaaagttgta gttagtcttc
     3061 cagtttgcta aatttctcga atagtatctt ttttaggtat gtgatttctt tttgcatttt
     3121 tgagttagat aaggtataat ggttttattg tcttttgggg tcgttacgga ttcgacaggc
     3181 attatgaggc atattttgcg actcgtgtgg cgacgtaaac gctcagttaa atataactgc
     3241 aaaaaataac acttcttacg ctctagctgc ttaaaaacca gcaggcgtga cccgatttgg
     3301 attgctcgtg ttcaatgaca ggtcttatta ttagcgagat acgattaagt cttgtctagc
     3361 ggcttgataa gagattgata gactcgcagt ttctagactt gagttatgtg tcgaggggct
     3421 gttaaaataa tacataacct atggttgtag acaaatatgt tggcaggtgt ttggacgtgg
     3481 gttcgactcc caccggctcc attattcctt tgcattcctt tgcattcttt tgcattcctt
     3541 ggtaaaacgt tgttaaatca acgtttttta tttttatctt tggtattcct ttgcattctt
     3601 ttgctaaaaa gggagtcaca aacagaccct attttaaaaa aaggatagaa aaaaggatac
     3661 aacatttgtc gcatcctaga aataatcttt tttcgacgga agacatggga ttcgaaccca
     3721 cgcacgctat tacacgccta ccgcgtttcc aacacggcct cttaagcctc ttgagtaatc
     3781 ttccaatact tactcaaata gtctaccata aaggctctta tcttgcaata aaaattctag
     3841 aaataagaaa aaatgataga ttttgaaaga aaatgataaa aaatgcttga cttcgaaaga
     3901 aagtatgata gaatgaatag tgtaaacgat aacaggaggt gattcagtgt taaaaacaga
     3961 acgtaaacaa ctaattttag aggagttaaa tcaacatcat gtagtttctc tagaaaaatt
     4021 agttagtttg ctagaaacgt cagaatcaac ggttcgaaga gacttggatg agttggaagc
     4081 ggaaaacaag cttcgtcgtg tgcatggtgg agcagaactc ccctactcct tacaggaaga
     4141 agaaaccatt caagaaaaat ctgtcaaaaa ccttcaagaa aagaaattgc tggctcagaa
     4201 agcagcctct ctcattaaag aaaaagatgt catctttatc gatgctggaa caacaactgc
     4261 ttttttgatt catgaattgg tcaataagaa tgttacagtt gtgaccaact ccattcacca
     4321 tgccgctcag ttggttgaaa agcagattcc aactgtcatg gttggaggaa acgtcaagac
     4381 ggcgacagat gctagtatcg ggggcgttgc tcttaaccag attaaccaat tgcactttga
     4441 ccgtgccttt atcggaataa atggtgttga cgatggctat tatacgactc ctgatatgga
     4501 ggagggagct gtgaaaagag ctattttgga gaatgccaag cagacctacg tcttggtgga
     4561 ttcgtcaaaa attggacaaa cttgctttgc caaggtagcc ccactcaaac gcgctatcgt
     4621 tatcactagt caagggcatg agctcttgca ggttattaag gagaaaacgg aggtaataga
     4681 agtatgattt atacagtcac actcaatcca tccattgact atatcgttcg tttggaccaa
     4741 gtcaaagttg gtagtgtcaa tcgtatggac agtgatgata agtttgctgg tgggaaagga
     4801 atcaatgtca gccgtgtctt gaaacgtttg aatataccaa atacagcgac gggatttatc
     4861 ggtggcttta ctggtaaatt tatcacagat actttagcag aggaagaaat cgagacacgt
     4921 tttgtccagg tggcagaaga tactcgtatc aatgttaaaa tcaaagcaga ccaagaaaca
     4981 gaaatcaacg gaacgggtcc aactgttgaa tcggttcagc tagaagaatt gaaagctatt
     5041 ttatctagtc tgacagcaga agatacagtt gtctttgcag gttcaagtgc taaaaatcta
     5101 ggcaatgtta tctataagga tttgatttcc ttgacgcgcc agactggtgc gcaagtggtc
     5161 tgtgactttg aaggacagac cttaattgat agtttggact accagcctct tcttgtaaaa
     5221 ccaaacaatc atgaacttgg agcgattttt ggggttaaac tcgaaagttt agatgaaatt
     5281 gagaaatacg ctcgtgagtt actggctaag ggtgctcaaa atgttattat ctctatggct
     5341 ggtgatggtg cccttcttgt cacatctgag ggagcttact tcgctaaacc aatcaaagga
     5401 acagtcaaaa attcagttgg agctggtgat tctatggttg ctggattcac aggtgaattt
     5461 gtcaaatcaa aagacgcagt agaagccttc aaatggggag tggcttgcgg aacggcaact
     5521 accttctcag atgacttggc aacggcggaa tttattaaag aaacatatgg aaaagttgag
     5581 gtagaaaaac gatgaaaatt caagacctat tgagaaaaga tgtcatgttg ctagatttgc
     5641 aggcaactga aaaaacagct gtcatcgacg agatgattaa aaatttgaca gaccacggtt
     5701 atgtaacaga ttttgaaaca tttaaagaag gaattttggc gcgtgaagct ttgacttcta
     5761 ctggtttggg tgatggaatc gcaatgcctc acagcaaaaa cgctgctgtc aaagaagcga
     5821 cagttctatt tgctaagtca aataagggtg ttgactacga gagcttggat ggacaagcaa
     5881 ctgacctctt cttcatgatt gcagctccag aaggtgccaa tgatactcac ttggcagcct
     5941 tggcagaatt gtctcaatac ttgatgaaag acggttttgc agacaaactt cgtcaagcaa
     6001 catctgcaga ccaagttatc gaactttttg accaagcttc agaaaaaact gaggaacttg
     6061 ttcaagcacc tgctaatgac tctggtgact ttatcgtagc tgttacagct tgtacaacag
     6121 gtattgccca cacttacatg gcccaagaag cccttcaaaa agtagctgct gaaatggggg
     6181 ttggtatcaa ggtcgaaacc aacggtgcta gcggtgttgg aaatcaacta actgcagaag
     6241 atatccgtaa ggctaaagct attatcattg cagcagacaa ggccgttgaa atggatcgat
     6301 ttgatggaaa accattgatc aatcgtccag ttgctgacgg tatccgtaag acagaagagc
     6361 taattaactt ggctctttca ggagatactg aagtctaccg tgccgctaat ggtgcaaaag
     6421 ctgcaacagc ctctaacgaa aaacaaagcc ttggtggtgc cttctacaaa cacttgatga
     6481 gtggtgtatc tcaaatgtta ccattcgtta tcggtggtgg tatcatgatt gcccttgcct
     6541 tcttgattga cggtgctttg ggtgttccaa atgaaaacct tggcaatctt ggttcttacc
     6601 atgagttagc ttctatgttc atgaaaattg gtggagctgc ctttggtttg atgcttccag
     6661 tctttgcggg ttatgttgcc tactctattg ctgaaaaacc aggtttggta gcaggtttcg
     6721 tggctggtgc tattgccaaa gaaggttttg cctttggtaa aattccttat gccgcaggtg
     6781 gtgaagcaac ttcaactctt gcaggtgtct catctggttt cctaggtgcc cttgttggtg
     6841 gatttatcgc aggtgccttg gttcttgcca tcaagaaata cgttaaagtt cctcgttcac
     6901 tcgaaggtgc taaatcaatc cttctattgc cacttcttgg aacaatcttg acaggatttg
     6961 ttatgctagc tgtaaatatc ccaatggctg caatcaacac tgctatgaat gacttcctag
     7021 gcggtcttgg aggaggttca gctgtccttc ttggtatcgt ccttggtgga atgatggctg
     7081 ttgatatggg tggaccagtt aataaagcag cttatgtctt tggtacaggt acgcttgcag
     7141 caactgtttc ttcaggtggt tctgtagcca tggcagcagt tatggctgga ggaatggtgc
     7201 caccacttgc aatctttgtc gcaactcttc ttttcaaaga taaatttact aaggaagaac
     7261 gtaactctgg tttgacaaac atcatcatgg gcttgtcatt tatcactgag ggagcgattc
     7321 catttggtgc cgctgaccca gctcgtgcga ttccaagctt catccttggt tcagcagtag
     7381 caggtggact cgttggtctt actggtatca aactcatggc gccacacgga ggaatcttcg
     7441 ttatcgccct tacttcaaat gctctccttt acctcgtttc tgtcttggta ggagcaatcg
     7501 taagtggtgt ggtttatggt tacctacgca aaccacaagc ataaaaaata gaaaaatgaa
     7561 aagattgaac cgtttggtgc agtctttttc tcttcccgaa atgcctgtga aatatggtat
     7621 aatagaagaa tggcaaacaa gaatacaagt acaacaagac ggagaccgtc taaagcagaa
     7681 ctggaaagaa aagaagcgat tcaacgaatg ttgatttcgt taggaattgc gattttattg
     7741 attttcgcag ccttcaaatt aggggctgca ggtataaccc tttataattt aattcgcttg
     7801 ctagtgggta gcctagctta tctggcgata ttcggcctat taatctatct cttctttttc
     7861 aagtggatac gaaaacagga aggactctta tctggctttt tcaccatatt tgctggctta
     7921 ctcttgattt ttgaagccta cttggtttgg aaatatggtt tggacaagtc cgttctaaaa
     7981 gggaccatgg ctcaggttgt gacagatctg actggttttc gaacgactag ctttgctgga
     8041 gggggcttga tcggggtcgc tctttatatt ccaacagcct ttctcttttc aaatatcgga
     8101 acttacttta ttggttctat cttgatttta gtgggttctc tcctagtcag cccttggtct
     8161 gtttacgata ttgctgaatt tttcagtaga ggctttgcca aatggtggga agggcacgag
     8221 cgtcgaaaag aggaacgctt tgtcaaacaa gaagaaaaag ctcgccaaaa ggctgagaaa
     8281 gaggctagat tagaacaaga agagactgaa aaagccttac tcgatttgcc tcctgttgat
     8341 atggaaacgg gtgaaattct gacagaggaa gctgttcaaa atcttccacc tattccagaa
     8401 gaaaagtggg tggaaccaga aatcatcctg cctcaaactg aacttaaatt ccctgaacag
     8461 gaagatgact cagatgacga agatgttcag gtcgattttt cagccaaaga agcccttgaa
     8521 tacaaacttc caagcttaca actctttgca ccagataaac caaaagatca gtctaaagag
     8581 aagaaaattg tcagagaaaa tatcaaaatc ttagaagcaa cctttgctag ctttggtatt
     8641 aaggtaacag ttgaacgggc cgaaattggg ccatcagtga ccaagtatga agtcaagccg
     8701 gctgttggtg taagggtcaa ccgcatttcc aatctatcag atgacctcgc tctagccttg
     8761 gctgccaaag atgtccggat tgaagcacca attcctggga aatccctaat cggaattgaa
     8821 gtgcccaact ccgatattgc cactgtatct ttccgagaac tatgggaaca atcgcaaacg
     8881 aaagcagaaa atttcttgga aattccttta gggaaggctg ttaatggaac cgcaagagct
     8941 tttgaccttt ctaaaatgcc ccacttgcta gttgcaggtt caacgggttc agggaagtca
     9001 gtagcagtta acggcattat tgctagcatt ctcatgaagg cgagaccaga tcaagttaaa
     9061 tttatgatgg tcgatcccaa gatggttgag ttatctgttt acaatgatat tccccacctc
     9121 ttgattccag ttgtgaccaa tccacgtaaa gccagcaagg ctctgcaaaa ggttgtggat
     9181 gaaatggaaa accgttatga actctttgcc aaggtgggag ttcggaatat tgcaggtttt
     9241 aatgccaagg tagaagagtt caattcccag tctgagtaca agcaaattcc gctaccattc
     9301 attgtcgtga ttgtggatga gttggctgac ctcatgatgg tggccagcaa ggaagtggaa
     9361 gatgctatca tccgtcttgg gcagaaggcg cgtgctgcag gtatccacat gattcttgca
     9421 actcagcgcc catctgttga tgtcatctct ggtttgatta aggccaatgt tccatctcgt
     9481 gtagcatttg cggtttcatc aggaacagac tcccgtacga ttttggatga aaatggagca
     9541 gaaaaacttc ttggtcgagg agacatgctc tttaaaccga ttgatgaaaa tcatccagtt
     9601 cgtctccaag gctcctttat ctcggatgac gatgttgagc gtattgtgaa cttcatcaag
     9661 actcaggcag atgcagacta cgatgagagt tttgatccag gtgaggtttc tgaaaatgaa
     9721 ggagaatttt cggatggaga tgctggtggt gatccgcttt ttgaagaagc taagtctttg
     9781 gttatcgaaa cacagaaagc cagtgcatcc atgattcagc gtcgtttgtc agttggattt
     9841 aaccgtgcga cccgtctcat ggaagagctt gagatggcag gtgtcattgg tccagctgaa
     9901 ggtaccaagc caagaaaagt attgcagcaa taaaaaatag cttctttcca aggttggaag
     9961 gaagctattt ttctggttat tgattacttt tattttctga agttggagta ttgggctgtt
    10021 tttcattttc agtggcaggt ttacttgtag caggtgtagt agagtcctga gttgctgttt
    10081 tctgatcttc ttttttctct tccttgacgc tagattttgg cgtttcttct tgttgtgttt
    10141 tttcttgaga agtgttattt tccttatttg gagtagtatt ttcctgaggg gattcttttt
    10201 gaatttcttt gacaaaggtt gtcgtctggc ttgtcgtagg ttctttttta gtatttttat
    10261 tattatccaa ggcgttcatg atcgtgatac ccgcggtaat caaaccaagg atactaccga
    10321 tagtagtaat ggttttttga aaaacagtaa agcctttttt gatatgttct gttttcggtt
    10381 ttgaagttct acgatagtta gacatacatt ctctcctttg attatgactt attatactct
    10441 gtttctttaa aaaaatctta aaaaaagaga aattgccctt tcttgtcgca agctccgttt
    10501 catgatacaa tagagggagt gattttagaa agcgtgagaa acatgattta ctttgataat
    10561 tcagcgacga ccaagcctta tcctgaagcc cttgaaacct atatgcaggt cgcttcaaaa
    10621 attttaggaa acccatctag tctccatcgt ttgggagacc aagcaacacg aatcttagat
    10681 gcttctcgcc aacagattgc agatttagtc ggtaagaaaa gcgatgaaat cttctttacc
    10741 tctggtggaa cagaagggga taactgggtt atcaagggtg tggcctttga aaaagctcag
    10801 tttggcaagc acatcattgt ttcagccatt gaacatccag cagtcaaaga gtcagccctc
    10861 tggttgaaaa gtcaaggatt tgaagtggat tttgctccag ttgataagaa aggcttggtc
    10921 gatgttgagg cgttagcagg tttgatacgg cctgatacaa tcctcgtttc catcatggct
    10981 gtgaacaatg aaatcggctc tatccaacct attgaggcta tttcagaatt cttggcagac
    11041 aagccgacta tttccttcca cgttgatgcg gttcaggcgc ttgccaaaat tccgactgaa
    11101 aagtatctga cagaacgggt ggattgcgcg accttctcga gtcataaatt tcatggtgtc
    11161 cgtggtgttg gctttgtcta tatcaaatct ggcaagaaga ttacacctct tcttacaggt
    11221 ggtggccagg agcgagatta tcgttcgaca actgaaaatg tggcagggat tgcagcgaca
    11281 gccaaggctc ttcgtttatc tatggaaaag ctagatatct ttaggagcaa gactgggcag
    11341 atgaaggcag tgattcacca agctcttctg aactatccgg atatttttgt cttttcagat
    11401 gaggaagact ttgcacctca tattctgact tttggaatca aaggtgttcg aggtgaagtc
    11461 atcgttcacg cctttgaaga ctatgatatt tttatctcaa caacctcagc ttgttcatct
    11521 aaggcaggaa aaccagctgg taccttgatt gccatgggag tggacaaaga taaggccaag
    11581 tcagctgtgc gtcttagcct agacttggaa aatgatatga gtcaggtcga gcagtttttg
    11641 accaagttaa aattgattta caatcaaact agaaaagtaa gataggagca ttcatgcagt
    11701 attcagaaat tatgattcgc tacggagagt tgtcaaccaa gggtaaaaac cgtatgcgtt
    11761 tcatcaataa acttcgtaat aatatttcgg acgttttgtc tatctatact caagttaagg
    11821 taacagcaga tcgcgaccgt gcccacgctt acctcaatgg agctgattac acagcagttg
    11881 cagaatctct caaacaagtt tttggaattc aaaacttttc tcctgtttat aaggttgaaa
    11941 aatctgtaga agttttgaag tcttctgtcc aagagattat gcgggacatc tacaaggaag
    12001 gtatgacctt taagatttct agcaagcgta gcgaccacaa ctttgaactt gatagtcgtg
    12061 aactcaacca aacacttgga ggggctgtat tcgaagccat tccaaatgtg caagttcaaa
    12121 tgaaaagtcc tgacatcaat cttcaggtgg agattcgtga agaagcagcc tatctttctt
    12181 atgaaaccat tcgtggggct ggtggtttgc cagttggaac ttcaggtaaa gggatgctca
    12241 tgttgtcagg agggattgac tcacctgtag caggttatct tgctcttaag cgtggggtgg
    12301 atatcgaggc agttcacttt gctagtccac catatactag tcctggtgcc ctcaagaaag
    12361 cgcaggactt gactcgtaaa ttgaccaagt ttggcggaaa tatccagttt atcgaggtgc
    12421 ctttcacaga gattcaagag gaaatcaaag ccaaagcgcc agaagcttat ttgatgactc
    12481 taactcgtcg ctttatgatg cggattactg accgtattcg tgaggtacga aatggtttgg
    12541 ttatcatcaa tggggaaagt ctaggtcaag tagccagcca aacccttgaa agtatgaagg
    12601 ctatcaatgc tgttaccaac actcccatca ttcgtcctgt ggttaccatg gacaagttgg
    12661 aaatcattga catcgcccag gaaatcgata cctttgacat ttcaatccaa ccgtttgaag
    12721 actgttgtac catttttgca ccagatcgtc caaaaacaaa tcctaaaatt aagaatgcgg
    12781 agcagtacga agcgcgtatg gatgttgaag gcttggttga gcgagcagtg gctggaatca
    12841 tgattactga aatcacacct caag
//
LOCUS       DAVAAL010000044        12338 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVAAL010000044 DAVAAL010000000
VERSION     DAVAAL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12338
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.045"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..324
                     /locus_tag="VQM60_001076"
     CDS             <1..324
                     /locus_tag="VQM60_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HET1080951.1"
                     /translation="LTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWK
                     EGQASTASSSNDSNSEYSAALGKAKTYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAI
                     DHLDD"
     gene            451..1410
                     /locus_tag="VQM60_001077"
     CDS             451..1410
                     /locus_tag="VQM60_001077"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET1080952.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            1507..2346
                     /locus_tag="VQM60_001078"
     CDS             1507..2346
                     /locus_tag="VQM60_001078"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET1080953.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(2393..2563)
                     /locus_tag="VQM60_001079"
     CDS             complement(2393..2563)
                     /locus_tag="VQM60_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET1080954.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            2684..3568
                     /gene="miaA"
                     /locus_tag="VQM60_001080"
     CDS             2684..3568
                     /gene="miaA"
                     /locus_tag="VQM60_001080"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET1080955.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVLHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            3561..4799
                     /gene="hflX"
                     /locus_tag="VQM60_001081"
     CDS             3561..4799
                     /gene="hflX"
                     /locus_tag="VQM60_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET1080956.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            4792..5415
                     /locus_tag="VQM60_001082"
     CDS             4792..5415
                     /locus_tag="VQM60_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET1080957.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            5430..6359
                     /locus_tag="VQM60_001083"
     CDS             5430..6359
                     /locus_tag="VQM60_001083"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET1080958.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            6380..7135
                     /locus_tag="VQM60_001084"
     CDS             6380..7135
                     /locus_tag="VQM60_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET1080959.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(7189..8097)
                     /locus_tag="VQM60_001085"
     CDS             complement(7189..8097)
                     /locus_tag="VQM60_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET1080960.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            8161..8430
                     /locus_tag="VQM60_001086"
     CDS             8161..8430
                     /locus_tag="VQM60_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET1080961.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            8430..8810
                     /locus_tag="VQM60_001087"
     CDS             8430..8810
                     /locus_tag="VQM60_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET1080962.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            8899..9126
                     /locus_tag="VQM60_001088"
     CDS             8899..9126
                     /locus_tag="VQM60_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1080963.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(9166..9891)
                     /locus_tag="VQM60_001089"
     CDS             complement(9166..9891)
                     /locus_tag="VQM60_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET1080964.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLKGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDSGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            10053..11894
                     /gene="typA"
                     /locus_tag="VQM60_001090"
     CDS             10053..11894
                     /gene="typA"
                     /locus_tag="VQM60_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET1080965.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            11914..12168
                     /locus_tag="VQM60_001091"
     CDS             11914..12168
                     /locus_tag="VQM60_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET1080966.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT         3590 a         2364 c         2804 g         3580 t
ORIGIN      
        1 ttgacatcag atggttctta tgcttacagt acatggcaag gaaattacta tctaaaatcg
       61 gatggtaaaa tggctgtcaa tgaatgggtt gatggtggac gttattatgt tggcgctgac
      121 ggagtttgga aggaaggtca agcaagtaca gcttcttcta gtaatgatag caatagtgaa
      181 tattctgctg ctttaggaaa ggcaaagact tataactcac tattccatat gtcaaaaaaa
      241 cgcatgtata gacaattaac ttctgatttt gataaatttt caaatgatgc agctcaatat
      301 gccattgatc atttagatga ttaacagata ctggtaggcg aaaaaattcc ttataattca
      361 tttacgcaaa cctttgcata agtgaatgga ttttgatata ctagtactgt aagcattttc
      421 aattactaca caatgaaaaa ggagaatatg atgagtcaaa agattattgg gattgacctt
      481 ggcggaactt ctatcaaatt tgcaatctta acaacagcag gagaaatcca aggaaaatgg
      541 tcaatcaaga ccaacatttt ggatgaggga agtcatatcg ttgatgatat gattgagtct
      601 attcaacatc gtttggactt gcttggattg gcagcagcgg acttccaagg cattggaatg
      661 ggatcaccag gtgtggttga ccgtgacaaa gggactgtta tcggtgccta caacttgaac
      721 tggaaaaccc ttcaaccaat caaacaaaag attgaaaaag ctttgggcat tccatttttc
      781 atcgataatg atgccaacgt agcagctctt ggtgagcgct ggatgggtgc tggtgataac
      841 caaccagatg ttgtctttat gacactcggt actggtgttg gtggtggtat tgtcgcagaa
      901 ggcaaattgc ttcatggtgt tgctggtgca gcaggtgaac ttggtcatat cactgttgat
      961 tttgaccaac caattgcatg tacttgtggt aaaaaaggct gtcttgagac agtcgcttct
     1021 gcaacaggaa ttgtcaattt gactcgtcgc tatgccgatg aatacgaagg cgatgcagcc
     1081 ttgaaacgct tgattgataa cggagaagaa gtaactgcta agactgtctt tgatctcgca
     1141 aaagaaggag acgaccttgc tttgattgtt taccgtaact tctcacgtta cttgggaatc
     1201 gcttgtgcta acattggttc aatcctaaac ccatcaacaa tcgtcatcgg tggtggtgtg
     1261 tcagctgcgg gagaattcct tctacaaggt gttcaaaaag tttacgatga aaatagtttc
     1321 ccacaagtac gcacatctac taaattagct cttgcaactc taggaaatga cgctggagtt
     1381 atcggagcag catcacttgt attgcaataa gataatgaga gaggaaaatg ctaacctaga
     1441 gttagtaatg ttcctctttt attttttatg ctatactagt taggacaatg aatgaggtga
     1501 gagtaaatga caaaagcaga tacgattttt aaagagaata ttgaacgcat tctaaaggaa
     1561 ggtgttttct cagagcaggc tcgtcctaag tacaaggatg ggactgttgc taattccaag
     1621 tatgtaactg gtgccttttc cgagtacgac ttatctaaag gagaatttcc tatcacaacc
     1681 ttgcgtccca ttgcaatcaa atccgccatc aaagaagttc tctggattta ccaagaccag
     1741 tcaaatagct tagaagtgct taatgacaag tacaatgttc actactggaa tgactgggaa
     1801 gttggagaca cgggaaccat tggtgagcgc tatggtgccg ttgttaagaa acacgacatt
     1861 atcaataagc ttctcaaaca gttggaaacc aatccttgga accgccgcaa tattatttct
     1921 ctctgggatt accaagcttt cgaagaaaca gatgggctgc tcccgtgcgc ctttcagacc
     1981 atgtttgatg ttcggcgtgt tgatggggaa atctatctgg atgcgacctt gacccagcgc
     2041 tccaatgata tgctggtggc ccaccacatc aacgctatgc agtatgtggc tttgcagatg
     2101 atgattgcca aacattttgg ctggaaggtt gggaagttct tctacttcat caacaacctc
     2161 catatctatg ataatcaatt tgaacaagct caggaattgc tccgtcggga gccgtcaaac
     2221 tgccaaccac gcttggtttt aaatgttcct gatgggacta atttctttga tatcaaagca
     2281 gaagattttg agttggtgga ttatgaccct gttaagccac agttgaagtt tgacctagct
     2341 atttaaaaga atagaaaaag aagttgagaa taatcccaac ttcttttgtt tcttaacgtg
     2401 atacgcggcg acgagctgct tttttacggt tttcttcgat gaaagctgct ttttgctctt
     2461 ctggttcgat tactttcttt ttaaatgcgt atactgcacc tgcaacggca gcgacagttc
     2521 ctgcgacacc tgttacaaga cctttagcga atcctttagc catgagtctt cctcctttat
     2581 attctcaatc agccagcctc ctcaagaggt cacatttttc tgactgacct ttttgtgtta
     2641 taataatagt aacgaaaaaa tgggaatttt tcaaggaaaa aagatgaaaa caaaaataat
     2701 tgtgattgtt ggaccgactg ctgttggaaa gacggctcta gctattgaag ttgcaaagcg
     2761 ttttaatggc gaagtggtta gtggagatag ccaacaagtc tatcgaggac ttgatattgg
     2821 gacggctaag gctagtccag aagagcaggc agctgttctt catcatttaa tcgatgttag
     2881 agagataacc gagtcttact cggcttttga ttttgtttca gaagctaaga tgactattga
     2941 ggatattcac agccgtggca agctagccat tatcgccggt gggactggac tttatatcca
     3001 gagcttgcta gaaggttacc acctaggtgg ggagactcct catgaggaga ttttagctta
     3061 tcgagctagt ttggagccat attcagatga ggaattagcc catttggtgg agcaagcagg
     3121 ccttgagatt ccccagttta atcgtcgtcg tgctatgcgt gccttagaaa ttgcccattt
     3181 tggtcaggat ttggaaaatc aagagacctt gtatgaaccg ctgattatct gcttggatga
     3241 tgaacgtagt caactttatg agcgtatcaa ccaccgagtg gatttgatgt ttgaggctgg
     3301 gcttttggat gaggccaagt ggctgtttga ccattcccct aatgtacagg ctgctaaagg
     3361 aattggctat aaggaactct ttccttattt ccgtggagag cagacctttg aggaagctag
     3421 tgagagtctt aaacaggcga cccgtcgttt tgccaagcgt cagctgacct ggttccgtaa
     3481 tcgcatgcag gtcacctttt atcagattgg agaatctggt gtacaagacc gcattttaag
     3541 ccagatagag gagtttttag atgattgaaa cggagaaaaa agaggagcga gtcctgctga
     3601 ttggtgtgga attgcagggt atggacagtt ttgacctctc catggaagaa ttggctagtt
     3661 tagcgaaaac ggcaggggca gtcgttgtag atagctacag acaaaaacgt gaaaaatatg
     3721 attccaagac cttcgtcggc tctggtaagt tggaagagat tgcgcttatg gtggatgcag
     3781 aagaaatcac tactgtcatc gtcaacaatc gtctgacccc aaggcagaat gtcaatctag
     3841 aggaagttct cggtgttaag gtcattgacc gtatgcagtt gattttggat atctttgcca
     3901 tgcgggctcg aagccatgaa gggaagctcc aagtccacct agcccaactc aaataccttt
     3961 tgcctcgctt ggttggtcag gggattatgc tcagccgtca ggcaggggga attggttccc
     4021 gtggtcctgg tgaaagccag ctggagctga accgtcgtag cgttcgcaat caaatcacgg
     4081 atatcgagcg ccagctcaag gtggttgaga aaaatcgggc gactgtcaga gaaaaacgtt
     4141 tggagtctag cacctttaag attggtttga ttggttatac caatgctggg aaatcaacta
     4201 tcatgaacat cttgaccagt aagacccagt atgaagcaga tgagctcttt gcgactctgg
     4261 atgcgacaac caagagtatt catctgggag gcaatctcca agtaactttg acagataccg
     4321 ttggctttat tcaagatttg ccgacagagt tggtgtccag tttcaagtca accttggaag
     4381 aaagcaagca tgtggacctt ctggttcatg ttatcgatgc tagcaatcct taccacgagg
     4441 agcatgaaaa aacggttctc tccatcatga aagacctgga catggaagat attcctcact
     4501 tgacgcttta taataaagcg gatttggtgg aggatttcac gcctacccaa acgccatata
     4561 ccctcatttc tgccaagtct gaggacagtc gtgaaaactt gcaagcatta ttgctagata
     4621 agattaagga aatttttgaa gcatttaccc tgcgagtgcc tttttcaaag tcctacaaga
     4681 ttcatgattt agagagtgtt gcaattctgg aagaacgtga ttatcaggaa gacggcgaag
     4741 tgattacagg ctacatttcc gagaaaaata aatggaggtt agaagaattt tatgactgat
     4801 attaaaactt tggctctaaa gtatggaggt tatacaagtc tggacaaggt ctatctggat
     4861 cagcttctag ctggtaaaac agagcaggag cagttaacac ttattacacc tccgccaagt
     4921 gttgttaatg cctactttgc agaactctat cagaaaaaaa gtcctcaggc tgcgacggat
     4981 tattttgcag aactcagtca ggaactgaat ctttacaata ctgaaccaag tttcacccta
     5041 gaaaataagc cttttattcg gcttaatctg tctggtaaat cctttggttt ttgttatgag
     5101 agtgacggct ttggtcggat tttctctgaa aatgaggaga aaatctcgga tgacttgctc
     5161 tttgaaattg cgcaaatttt ctcccatcaa ttagtctttg aagagtctgg taagatttac
     5221 atgaagcctg tcaaggacga ggaagttgtc agcctagaaa gtctcacagc tttgactgat
     5281 ttggaaagat tggctgatgg tcgaaaacgt ctcaaaggct acagccaaga ggatttattg
     5341 caagaagctg ctgctttttc tggcaaacgc tatttccgat cggaaaaccg cacagccatg
     5401 ttatatattg attaattaga aagtatcgaa tggatattca atttttagga acgggggctg
     5461 gtcagccctc taaagcccgc aacgtttcaa gtctcgccct gaaacttttg gacgagatta
     5521 acgaagtttg gctctttgac tgtggagaag gtacgcaaaa tcgcattctg gaaaccacaa
     5581 ttcgaccacg taaggtcagc aaaatcttta ttacccatct gcatggagac cacatttttg
     5641 gtttgccagg tttcctttct agccgtgcct ttcaggccaa tgaagagcag acagatttgg
     5701 aaatctacgg acctcaagga atcaagtcat ttgtcttaac cagccttcgt gtgtcaggtt
     5761 ctcgtctgcc ctaccgcatt catttccatg agtttgacca agattctctg ggtaaaattc
     5821 ttgaaaccga taaattcact gtgtatgcag aggagctgga ccacactatt ttctgtgttg
     5881 gctatcgtgt catgcaaaag gatctagaag ggacgctgga tgctgaaaaa ctcaaggctg
     5941 ctggtgttcc gttcggcccg ctttttggta aaatcaaaaa cggccaggat cttgttttgg
     6001 aagacggaac tgaaatcaag gcagcagact atatctcagc gccacgtcca ggtaagatta
     6061 tcactatttt aggagacact cgaaaaacgg atgccagtgt gcgtctggct gtcaatgcag
     6121 atgtcctagt tcatgagtcc acttatggca agggtgatga aaaaattgct cgtaaccatg
     6181 gtcactcaac taatatgcaa gctgcacaag tagcggtaga agcaggtgcc aaacgcctcc
     6241 tactcaacca tatcagtgcc cgtttcctct caaaagatat aagcaaactc aagaaggacg
     6301 ctgccacaat ttttgaaaat gtccatgtgg tcaaagactt ggaagaagtg gaaatctagc
     6361 agtcacagaa aggataagta tgcctactat tctcattacc ggagctagcg gtggtctagc
     6421 tcaagaaatg gtcaaactcc tgcccaatga ccaactcatc ttgcttggta gaaataagga
     6481 aaaattagcc caactctacg gaaattattc ccatgcagaa ttgattgaaa ttgatattac
     6541 cgacgattca gccctagaag ctctggtaac tgatctttat ctccgctatg gcaagattga
     6601 tgtcttgatt aacaacgctg gttacgggat ttttgaggga tttgaccaga ttgctgataa
     6661 agatattcac cagatgtttg aggtcaatac ctttgccctg atgaatctgt ctcgtcacct
     6721 tgcggctcgt atgaaggaaa gcagcaaagg gcatatcatc aacatcgtca gcatggcagg
     6781 tctaatagct actggcaagt ctagtcttta ctcagcgacc aagtttgcgg ctattggttt
     6841 ttcaaatgct ctgcgactcg aacttatgcc ctatggagtc tatgtgacaa cagtcaatcc
     6901 aggtccaatc cgaacaggat tttttgacca agctgaccca gatggaactt atcttaaatc
     6961 ggttgaccgc ttcctcttag aggcagatgc agtggctaaa aagattgtca agattatagg
     7021 aaaaaataaa cgagaactca atctcccgat tttgttgaac ctagcccata agttttatac
     7081 tctctttccc aagctagctg ataagttggc aggggaaact tttaattata agtaaaaaga
     7141 accaatgtgc aggttgttgc tagcctacat attggttctt atcatttctc aactatcaaa
     7201 ctgaacttct tctagtagat agtcgataaa gcgttcgccc atcttagata ggctggtttt
     7261 ctcatgctgg atatagacca gctcaatcgg gtcatcaata tccagtggga tagaaacgat
     7321 attgtctccg tttaggttgc tgttcaaaat ccctgtcgca atggtataac catccaaacc
     7381 aatcaagaga ttaaagaggg tggcacggtc actgaccaca atggatttct tgtggtgttc
     7441 ttgagaaaga atctcttctg aaaagtagaa ggagttgtgc gtcccttggt catagctgag
     7501 gtaagggaaa ttctccaaat cagacagttt caccttgtct ttctttgcca gagggttggt
     7561 cttgctgaca aagatatgcg gttgcgctgt gaagagatgg tgggctagta ggtgattgtc
     7621 atccagcatc ttggttaaaa catcacggtt gtaactgttt aagaagagga ccccgacctc
     7681 actgcggaag ttcttgacgt cgtcgataat ctcccaagtc cgagtttcac gaaggaaaag
     7741 ctcgtatttc tccatatcgc ttttcttgag caaagagaca aaggcattga ccacaaaggc
     7801 atagtgttga gacgaaacgc taaagagttc gcggtgggcg acaggatttt tatagcgttc
     7861 ctccagaagc tgagtttgct cgacaacctg acgggcataa gagagaaact ccatcccatc
     7921 acgggtcaag gtgattccct tgggattgcg gataaagatc tcaatgccca tttcattttc
     7981 caaatctctc acagcattgg aaagacttgg ctgagtgata aagagttgct tggctgcctc
     8041 attcatagag ccagtttcga cgattttgat aatataatgt aattgttgaa ttctcatgtt
     8101 tttattgtac cacacttgcc ttagaatcgc caaagaagat aggaaaataa ggagcattgt
     8161 gtgagtctct tcttgaaaaa aacacaaaat tttgctagaa tgagtcttat gaaaacattc
     8221 tatgatgtgc agcaattcct caaacgattt ggtattattg tttacatggg aaaacgctta
     8281 tatgatattg aactgatgaa gttggaacta tctcggattt acgatgcagg gttgatggat
     8341 aaattagact atctagaggc agaagcagtt cttcgcagag agcacaaggt agaattggat
     8401 tatattgaga aaaatggaga aaagaactaa tggttacttg gattttgtgg gcacttatac
     8461 tagcaatgtt ggcgtggatg ggctttaact atcttcgtat tcgtcgtgcg gctaaaattg
     8521 tggacaatga ggagtttgaa gccttgattc gtacgggtca attgattgat ttgcgcgacc
     8581 cagcagaatt ccacagaaaa catatccttg gtgcacgcaa tattccttca agtcagttga
     8641 aaactagtct tgcagccctt cgtaaagata aacctgtcct tctctacgag aaccaacgtg
     8701 cgcaacgagt tacaaatgca gctctttact tgaaaaaaca aggtttttct gagatttata
     8761 tcctttctta tggcttggat tcttggaaag ggaaagtgaa gactagctaa ccttatttcc
     8821 aaattagtcg aatgatagga tgaagctatt gttttatagt ataattaatt tgtataaaat
     8881 ttaaagagtt cttagaacat gaaagaaaag aagaaaaatc cactttttgt gggtatcttg
     8941 tcgattattc ttggtttgtt atttccaatt gtaggcttga ttttgggtat cataggattg
     9001 gtcttggcta tttcatacca aaaagagtca caattagact ataaaataga aaagattctt
     9061 aatatcttag gaattgtgat ttctgtagtt aactggattg tagcaatcgc cttaattttt
     9121 cggtaaataa aagaaaaaag ctttagtttt aaagcttttt ttgttttatc gactcatctc
     9181 taaatagttt ttaatgattt gtaactcttt ttggttcaaa gggcggtagt tactttctgc
     9241 taaatctggg ttcaatgtga agtccccaaa ttggattctt ttgaggctag tcaccttaac
     9301 accaaccgag aggaacattt tcttgatttg atgaaatttt ccttctgaaa tggtgataga
     9361 ggcttgactg agggagggac ttgcagatag aatctctagt tttgcgggtt tacagacagt
     9421 accatctaaa aagacaattc ccttttgaaa ggcttggata tggtcaggtg ttagaagtcc
     9481 attaacctgg acttggtaag tcttatcgac atgatattgg ggatggagga gctgaaagcc
     9541 caagggaccg ctatcggtca agaggaggag tcccgttgta tctcggtcca gtcggccaac
     9601 ggcatagagc ttgtcagact ggatgtcaga tggaagcagg tccatgacgg tcggaagttc
     9661 cttgtctttg ttggctgtaa cgacaccagc aggcttatga agcataagat aggtgtgttc
     9721 atagccttga atgattcgat cctgaaaaag gagttcttgt agtcctgtat cgatattttg
     9781 agctagggat cgggctgggc aaccatcgac tagaatttcc cctttgagta aggcttgttt
     9841 catggccttt cggctgattt tttcttgagc taataaatta tctaaacgca tactgtgcct
     9901 atcttatcat aagtcgcttg ctttgaaaag gcttgacgtg aaaagaaaag tggagtgaaa
     9961 acatttacac ttgcttgagt tatgttattt acttgaaatt atggtataat cgttcagtta
    10021 gaaaataaat tttgaatatt atagaggaaa tcatgacaaa attaagagaa gatatccgta
    10081 acattgcgat tatcgcccac gttgaccacg gtaaaacaac cctggttgac gaattattga
    10141 aacaatcaga aacgcttgat gcacgtactg aattggcaga gcgtgctatg gactcaaacg
    10201 atatcgaaaa agagcgtggg attaccatcc ttgctaaaaa tactgccgtt gcttacaacg
    10261 gaactcgtat caacattatg gacacaccag gacacgcgga ctttggtgga gaagttgagc
    10321 gtatcatgaa aatggttgac ggtgttgtct tggtcgtaga tgcctatgaa ggaaccatgc
    10381 cacaaactcg tttcgtattg aaaaaagcct tggaacaaga ccttgtccca atcgtggttg
    10441 ttaacaaaat cgataagcca tcagctcgtc cagcagaagt agtggatgaa gtcttggaac
    10501 ttttcatcga gcttggtgca gatgacgacc agcttgattt cccagtggtt tatgcttcag
    10561 cgatcaacgg aacttcttca ttgtcagatg atccagctga ccaagaagcg actatggcac
    10621 caatctttga cacgattatc gaccatatcc cagctccagt agataactca gatgagcctt
    10681 tgcagttcca agtgtcactt ttggactaca atgacttcgt tggacgtatc ggtatcggtc
    10741 gtgtcttccg tggtacagtt aaggttgggg accaagttac cctttctaaa cttgacggta
    10801 caactaaaaa cttccgtgtt acaaaactct tcggtttctt tggtttggaa cgtcgtgaaa
    10861 tccaagaagc caaagcgggt gacttgattg ccgtttcagg tatggaagac atctttgtcg
    10921 gtgaaaccat cactccgaca gatgcagtag aagctcttcc aatcctacac atcgatgagc
    10981 caactcttca aatgactttc ttggtcaaca actcaccatt tgctggtaaa gaaggtaaat
    11041 gggtaacttc tcgtaaggtg gaagaacgct tgcaggcaga attgcaaaca gacgtttccc
    11101 ttcgtgttga cccaactgat tcaccagata aatggactgt ttcaggacgt ggagaattgc
    11161 acttgtcaat ccttatcgaa acaatgcgtc gtgagggcta tgaacttcaa gtatctcgtc
    11221 cagaagttat cgtaaaagaa atcgacggtg ttaaatgtga accatttgaa cgtgtacaaa
    11281 tcgacactcc agaagaatac caagggtctg ttatccaaag cctttctgaa cgtaaaggtg
    11341 aaatgttgga tatgatttca actggtaatg gtcaaactcg tttggtcttc cttgttccag
    11401 cgcgtggttt gattggatac tcaactgagt tcttgtcaat gactcgtggt tacggtatca
    11461 tgaaccatac cttcgaccag tacttgccat tgattccagg ggaaattggt ggacgtcacc
    11521 gtggtgccct tgtttctatc gatgctggta aggctacaac ttactcaatc atgtctatcg
    11581 aagaacgtgg tacgatcttt gtcaacccag gtactgaggt ttatgaagga atgatcatcg
    11641 gcgaaaactc tcgtgaaaat gacttgacag ttaacatcac gaaggcaaaa caaatgacca
    11701 acgttcgttc agctactaag gaccaaacag ctgttatcaa gacgcctcgt atcttgacac
    11761 ttgaagagtc tcttgagttc ttgaacgacg atgagtacat ggaagtaacg cctgagtcta
    11821 tccgtttgcg taaacaaatc cttaacaaag cagagcgtga gaaagctaac aagaagaaaa
    11881 aatcagctga ataagagcta gaaagagaga aagatggtct atttagtcct aggaatttta
    11941 ctgctcctac tctatgtatt tgcgacacca gaaagcatta aagggacagt caatatcgtc
    12001 gctatggtat gtattttagt ggcactcttg attttattgg ttctatcttt tctgaaaatt
    12061 tttcaattac caacagaaat attcctagca atagccatgt tgatcctagc ttactttagt
    12121 gtgagagaca tcacactcat gccagtcaaa aaaagtaaaa gaagataaaa aagagagttt
    12181 tggctctctt ttttgctagg aattttacaa gattgcaatt tatttttgag ttatcaccat
    12241 ctcttttgtt tggataatat gaatgggaaa aacttgaata aaattcttta atggaatcta
    12301 ttttatattt gcttagtact ggttgcatat tatttcga
//
LOCUS       DAVAAL010000045        12281 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVAAL010000045 DAVAAL010000000
VERSION     DAVAAL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.046"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(1..150)
                     /locus_tag="VQM60_001092"
                     /pseudo
     CDS             complement(1..150)
                     /locus_tag="VQM60_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(212..661)
                     /locus_tag="VQM60_001093"
     CDS             complement(212..661)
                     /locus_tag="VQM60_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET1080967.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(663..1421)
                     /locus_tag="VQM60_001094"
     CDS             complement(663..1421)
                     /locus_tag="VQM60_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET1080968.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1536..3515)
                     /gene="pknB"
                     /locus_tag="VQM60_001095"
     CDS             complement(1536..3515)
                     /gene="pknB"
                     /locus_tag="VQM60_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET1080969.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3512..4252)
                     /locus_tag="VQM60_001096"
     CDS             complement(3512..4252)
                     /locus_tag="VQM60_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET1080970.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(4267..5580)
                     /gene="rsmB"
                     /locus_tag="VQM60_001097"
     CDS             complement(4267..5580)
                     /gene="rsmB"
                     /locus_tag="VQM60_001097"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET1080971.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDISSIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5573..6508)
                     /locus_tag="VQM60_001098"
     CDS             complement(5573..6508)
                     /locus_tag="VQM60_001098"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET1080972.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6521..8917)
                     /locus_tag="VQM60_001099"
     CDS             complement(6521..8917)
                     /locus_tag="VQM60_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET1080973.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLVLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYYSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(8983..9297)
                     /locus_tag="VQM60_001100"
     CDS             complement(8983..9297)
                     /locus_tag="VQM60_001100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET1080974.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9322..9948)
                     /gene="gmk"
                     /locus_tag="VQM60_001101"
     CDS             complement(9322..9948)
                     /gene="gmk"
                     /locus_tag="VQM60_001101"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET1080975.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVSLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10078..11691)
                     /locus_tag="VQM60_001102"
     CDS             complement(10078..11691)
                     /locus_tag="VQM60_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET1080976.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLINEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11960..12190)
                     /locus_tag="VQM60_001103"
     CDS             complement(11960..12190)
                     /locus_tag="VQM60_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET1080977.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMDKIRAGH"
BASE COUNT         3283 a         2929 c         2431 g         3638 t
ORIGIN      
        1 gtgtcctcag aactgagagg agttcttgaa atcgtaacac cacctttgaa caagagttac
       61 ttcaacccat tggctccgac ggagtaagtt gctttcgtgg ataccaaaat cagccgcaat
      121 ttcttcataa gtgcggtatt ctcgcacata tagaaagcgt tgttaattta tttatctcat
      181 ttttcagaaa attcttttat ttctgtaaag tctacgatac tcgatgtgtt tttatataat
      241 gatacagtct gagaatcact gttccgctag ccattccaat agagattacc aaagccaaca
      301 tgacaaccaa ggtcgcactt gccagtgctt tattatagtc ccctgtcaca aaaaaggcag
      361 ttgttcggta ggagagataa cctggaacca gcggtgccaa aatggccaag ataaagacca
      421 cagcaggtgt cttataaaga atacttaaaa tctggctgac acaagaacca ataatggctg
      481 caatgaaggt agctacaatg acattggtcg gttccttgag caagagatag attagccaga
      541 cagtcatgcc caaaatccct ccaggtaaga gcatagaccg ttgcacattg agtacaatta
      601 aaaaagtgat aatggcaaga aaacttgcta ctgcttgtaa taaaaaggtt gttagtgtca
      661 tattagttca tcaataccaa ggcgacagaa gttcctgccc ctaaagcgag ggtaatgagc
      721 agggattcaa acatcttact cataccagag tttatgtggt tggtcataat atcacggacc
      781 gcattggtca aggcaatacc tggtacaaac ggcatgaccg caccagctat aatcaaatct
      841 gccgttgaag gaaaacctgt gtagcgagcc caaaactggg caattatccc aaagacaaag
      901 gctccagcaa aggctgtcac aaagggaatt cggataaatt tttccacata gagggaaaag
      961 gcaaaaccaa ataaggtcgc cactcctgcc ccaagtgcgt cgtagatatt tccgctaaac
     1021 ataactgaaa agaaaggagc actaaaggtc gcagccagag ttacctgcaa cttagtatag
     1081 ggaaggggtt gagcttgcaa ggccgtcaat tgcttaaagg ctgtttctaa gtcaatctgc
     1141 cccccaacta gctgacgaga aatctggttc acatcgcaga ctttttcaat gttataagaa
     1201 gaggaggtca cgcgcttcat gcgcgaaata ttggtatttt caatagagaa aaagatagcg
     1261 gcaggcatgg caaggacatt gcaatccaca atcccctgcg aatgcgcgat tcgaatcatg
     1321 gtatcttcta cacgatggat ttctgagcca cttttaagga gaatagtccc cgctagcata
     1381 atcacatcaa tgacggcatt taattctttt gattcttcca tgctttcctc cttttatcaa
     1441 ctccctctat tctatcacaa atccggactc aaaaaaaatc tttgccatga aatcatgaca
     1501 aagattgatt actcattttg attatccatc tgcttttaag gagtagctga agttgtttta
     1561 ggtttgtaga ttgaaatctt gactctagtc ttattgaggt ctaccttttc acctgctcta
     1621 ggactttgtt caacaaccat gccttctgca ctacctgcag gcgctgtcgt cacttctaca
     1681 acttctatat tagcttcctt aatcccaaca atttgaatca aattgttctt agtaaactcc
     1741 aagctggaac caatgtaact cggcatggca acacttgtaa cttttttagc tactgtcaag
     1801 acaatttgag taggtttact cacatcataa gtcgttccgg cacctggact ttgtttcata
     1861 atcgttcctg gttcgctttc gctggactct tcttcctcta tcttaatcaa attctcagga
     1921 accttcttct gcttaagttc tgagattact tctgtagagt tccgtccaat atagttccct
     1981 aattgaatcg tcgtagcttt tttagctact gtcaaaacaa tttgagttgc cttgctcaag
     2041 tcataggtcg taccttctgg tagactttgc ttcaagaccg ttccagcctc actctcattc
     2101 gactcttctt cctcaatttt aatcaaatta tctggaactt ttttctcttt taattccgcg
     2161 ataacatcag aagatttccg gccgacataa ttactaattt ggaaggattg tttgcctgat
     2221 gagacaacca aattgatttt cgttccttct tttcgaccag ttccagcgcc aggatctgta
     2281 cggataatcc gcccttcttc caccttttca ctagcctctg tcttctcctc accaatctca
     2341 aaattggctt ttttgagcgt tgccttggcc tctgcaactg tctgacctgc cacatctgga
     2401 atggcaatgg ttgcaggagt tctggatagt atccaaataa gagaagctgc caccaataca
     2461 aggctggcca acaaaatcag gtaacgcatc ttaaatctat gttttttcgg tgcttgtggt
     2521 tggtaagttt cctctgtcac agcctgactt gggtttttga ttgatttgtg ttctgtctgc
     2581 gcttgaacct taggaataga tgtcaaggta ctctgagaaa ccttcggcaa ggtcttggta
     2641 tctgccttgc tcgtttcatc aaagattaac ttactttcat ttctacgatt gtaggacaag
     2701 ctactagaca agtccacata catctctgaa accgagcggt agcgattggt caacttttta
     2761 gcagttgcct tgataataac attttctaaa gcctgaggta cagatggatt ttctgcaata
     2821 acggacggca ggggtttctg gaaatgctgg agggcaatgg tcaccgcgct atccccgtca
     2881 taagggatat ggcctgtcaa catctcatag aaaataatcc ccatggcata gatatcactc
     2941 tgcacagtcg ccttcgaacc acgcgcctgc tctggtgaca agtaatgaac tgagcccaac
     3001 atcgagttag tctgggtcag acttgtctct gcaaaggcta cagcaatccc aaagtctgtg
     3061 accttggcag tcccatctgg tgtcaaaagg atattttgag gtttcaagtc cctgtgaaca
     3121 attcctcgag tatgggccaa gcgcatagcc aagagaattt gtcccatgat acggactgct
     3181 tcttcattag aaagaggata atgttccttg atatagcgtt tgaggtccag tccagccaca
     3241 tactccatag ctaggtactg ttgaccgtct tcctcgccaa tatctgttat ccgaacgata
     3301 tgaggatggt ctagatctgc catagctctc gcttcacgct gaaaacgagc tacagctatc
     3361 gggtccgtct ggtagttggt cctcagaacc ttcactgcca cttcttcccc atctaagatt
     3421 aagtctttgg ctaggtagac atccgccatg cctcctcgac caatctgttt gacaatccga
     3481 tagcgtccgg caaaaatctt gccgatttgg atcattctgc atcctcctcg ttcatagaaa
     3541 caagggcaac cgtaatgttg tctaaacctc ctgcattgtt agcaaaacga acaagtgtct
     3601 ccgttttatc tgctaaagga atatcactgg ttacaatatc acgaatctca ctgcctgaaa
     3661 tcatgttggt caagccgtca ctattgagca agagatagtc acctgactca aggataactg
     3721 tcccaaaatc aggctgaatt tcatcttttt gcccaataga ctgggtgata atattttttt
     3781 gcggatgagc ttctgcctct tctggtgtca attgaccagc cttgagcaat tcattaacca
     3841 aggaatgatc gctcgtcaac tgatggtatt cttctccacg aatcaagccg atacgcgaat
     3901 caccaatatg agcatagata gcctgattat caataatagc aaggacttcc aaagtagttc
     3961 ccatgcctct gtaagcttca tcctgaccaa gctggtgaat cttttgattt tcaatttcta
     4021 ggtaatgggc gaaccattca cgcacttcat tgactgtatc gatctgggta tcaacccaag
     4081 ctacacccag gtctgtgacc gccatttcac tagcgatatt ccctgcacga tgacctccca
     4141 tcccatcagc taaaataatc atggtacgtc cagctctatt gacatagtgg ttgacatagt
     4201 cttggttatt tgttcgtttc tgaccaacat ctgttaataa tgaaatttcc atgtgtcagt
     4261 tccttcctaa tccgatatct tgcgaaattg actgatgaag aatccatcac ttccatacaa
     4321 ttcaggtgta atgaggatac agccgtcttt catgatatcc ttacattcat gttctagttt
     4381 tacctgctcg aactcgggat gactctctaa aaaggcctta acgacttgaa aattctcctc
     4441 tgagacgata gtgcaggtgc tataagttat tataccacct ttgcctagta tttgacaaac
     4501 actacctaat atttctaact gaatttcctg caaggacgcg aaatctgccg tttctttatt
     4561 gtatttgata tctggttttc ggcgcaaaag accgattcct gaacaaggag catccaccaa
     4621 aatcttatca aaggaatcct ggtcaaaaaa ctcatgcacc tttctggcat ccaatttttg
     4681 agtttgaacc cgatcttcaa ctcccagacg ttgggcattt tcttgaatta aatccaactt
     4741 gtggtcgtac aagtccagag cagtaacctg acctgtcgta agataagagg ctatatgggc
     4801 tgttttccca cctggagccg cacaggcatc aagcactcgc tcatcacctt gtaaatcaag
     4861 cgtcggagca accagctgac tggactcgtc ttggatggta atggctccat ccgcaaacaa
     4921 gttatgccct gcaaaatgcc cttgctcctt aaccagacca gtggttgcta aaagggaatt
     4981 attcgcctcc aacaaggctt ggatttcctc ttttcgactt aggtctgtta cacgaatact
     5041 ggctttgttt cgcactaaca agctttcaaa gatggctttt gctctctcct ctccgtattc
     5101 ttccttgagt ttggcaacta gccaaactgg gagagaatag gcaatggagt cacgcttgtt
     5161 ttttcgcttg atgctagaaa tatctggcca gccttcacgc aagatacggc gaaggagggc
     5221 attgaccaat ttttcactgc cttttttacg aagtttggcc aattccactg cttcattgac
     5281 cacagcatga tctggaatct tgtccaaata gcggagttgg taggcactca tgagaagaag
     5341 gacatagagc cagctgtcta actggtctct gtcttcgata aagtgggata ggtaccattc
     5401 cagagtcagt ttacgggcta ccgttccata gaccagctcg gtcactaagc ccttgtctgc
     5461 tgccaaaagt tgactcccct ttagatgctt atttaaggcg atatttgagt atgcttggtt
     5521 cacaaaaaca tcctctagca ctgctagagc taaacttcta gccgtttcta ctttagtcac
     5581 caaatcgttc tcctacagtc aatgtacgtc caactccgtt gaggaaggaa gcaatgtcca
     5641 tcttaggctt accagctggc tgcacttgtt tgagggatag agccccttca gccgttgcga
     5701 caatcaattc tttcttgccg atagagagaa tctcacctgg atttccctga ccttctactg
     5761 gtagggcttc ataaatctta aagcggtcgc ccttaaggaa agtatgggca acaggccagg
     5821 ggttcattcc acgaatttgg ttaaagagtt gacgattggt tttgttccag tccagttttt
     5881 cttcctctgg ctttatattt ggagagaagg taacctgact cgtatcctgc ggttcaggtt
     5941 tgatatcacc agcaatatag gcaggcagag tgtccaaaag caaatcacga ccaactagcg
     6001 ccaatttttc aaacaaggtg ccaacattgt cctcatctgt gatcggaatg ctgcgacgag
     6061 aaatcatatc tcctgcatcc atttccttaa ccatttccat gatggtcaca ccagcttcct
     6121 catccccttg aatcaaggca taatggatag gcgcaccacc acggtgtcta ggaaggaggg
     6181 aggcatgaac gttgacagca aagtccatgc tatcaaggag tttgcttggg agaaactgcc
     6241 caaaagcagc agtcacaatt ccatctgctc ctagcttcat aagatcttcc atctctggac
     6301 ttccagataa tttttcaggt tggtagatag atagtcctgc ttccttggca gcctgcttga
     6361 ctggggtttc ttggataact tttttacgac caacagcacg gtctggctgg gtcacaacgg
     6421 ctagaatttc gtaacggtca tctgtcaaaa gtccttttaa gactgttgct gaaaagtcgg
     6481 gggtccccat aaagattagt tttgtcatat cttctccttc ttataaaaat tgctgcggct
     6541 catggtcaat gctgagacgg agctcactat tttcccgttc ttgagtcaag gccaagacct
     6601 ggttgagggt cggccccagc tcatcttcta aacggtattt aattaaaatc tggtaatgat
     6661 agaggttgtg ggtacgggca atcggttttg gcgttggccc cagaatggga ctggtctctg
     6721 acaagcctga ccgcaaaatg ttcatgactt cataggcacg tttgaaaacc tcttcttctt
     6781 tcttgtgaga aagggtaata ccaatcgtga aatagtaagg tggatagccg agttgtcgtc
     6841 tgattcccat ttcataggca taaaagcctt cgtaatcttg atccttggca aatcgaatag
     6901 catagtgctg cgaattgtag gactgtatca agacttgacc tgctttttca gcccgacctg
     6961 ctcgacctgc cacctgagtc aagagctgga aggttctctc agaagaacgg aaatcaggca
     7021 gattcaaggc cgtatccgca tttagaactc cgactagggt aacattggga aaatccaaac
     7081 cctttgcaat catctgagta ccaagtaaaa tatccgcttc ccctcgccca aactggtcaa
     7141 gcaaggcttg gtgactgcct ttctttcgag tcgtatccac atccatcctc aaaatgcgag
     7201 cttggggaaa gagttctgct agctcatcat aagccttctg agttcccgtc ccatagtaac
     7261 gaatactgcg gctcttacag ttaggacaga cctgaggaat atccttcgag aaaccacaat
     7321 aatggcagtt catagtcttg gtatccatat gcaaggtcag agaaatatcg cagttgggac
     7381 aagtatccac cgtcccacac tcccgacaca tgacaaagct ataataacca cggcgattga
     7441 gcatgagaac cacctgctct tttttaacca gacggtcttg gatagcctct agcaaaggag
     7501 gcgtaaagtt tgacgtctca ttttgtccga tatagtctcg aaagtcaatc acttgaacct
     7561 cagggattgt agccaaagga ttggcacgtt gggttagacg taagtgttga tagacgcctt
     7621 tgccagcacg tgcccggctc tctaagctcg gcgttgcaga tccaagtacc agagttgctt
     7681 gattatactg agcccgtaaa atagctacct ctctggcatg gtaacgggga ttgctgtcct
     7741 gcttataagc cgcttcatgc tcttcatcaa taatcatgac acccagattt ttcagaggag
     7801 caaagatagc agatctggca ccaacaacaa cttgggcatc gccacgctcc accttgcgcc
     7861 attcatcata cttttcacca ttggataatc ctgagtgaag aatggctacc ttgtccccaa
     7921 aacgtgctat aaaacgctcg gtcatctgag gagtcaagga aatctcaggt accagcaaaa
     7981 tagctgtctt gcccttatcc agggcacctt ggataatctg caagtaaacc tcggtcttcc
     8041 cacttcctgt aatcccttga agtagaaagg gaggttgaga actgccaata gaactcacaa
     8101 ccgcatcacg cgcctgtctt tgttctggat ttaactccaa aggtctactt gcttcaattc
     8161 cttcaaaata agcagccgag cgttgaactt ccttttggac tatggtaaca gcaccttgat
     8221 ccacaaagaa gttgacttgc tctcgcgagt aggactctaa caagctagcc aaggaagcgc
     8281 tctctggatg agacagcaga taatctctca gttccaactt tttcttggca cgtgtagaaa
     8341 tctcaacacc ttctaattga gcatggtcaa cctcatacca agactgggtc ttgaccttct
     8401 tttgatcgac tgcctgatat tccagaacaa gcaggccttt tctagtcaaa cgcatcattt
     8461 cagcttgctt ggcaaggtct agtgaagaaa aggctagcga atcttctgaa ccaaacaggc
     8521 gcactcgttc ttcctgactc aagccttcca gaggatagag aatcttgtca tagctagaat
     8581 tcagaaatcc tggaagcatg gccttgagga tagaaatttt gtaggagaag acagatttgc
     8641 gtaactcctc agccagccag agttgttctg gcgtgagaac aggagaaaaa tccagcacct
     8701 ctgcaatatc ttttaaatct tgctccatct cttctccatc tgattgggac tccaaaccaa
     8761 gaacaatccc ttgaatcagg cgattaccct taccaaaagg cacatgaacc cgcatcccaa
     8821 cttccagcat tccctcaaat tcctccggaa tcctgtaact atagggctgg tccgtctgca
     8881 tcaagggcac atctacgata atcttagcta gggccatctt ctcacctcct ccttgtcagt
     8941 acattcttgc aatagaaaaa ataagattga gtccccccaa ccttaaattt tttcaccatc
     9001 ttctttttct ttagcaattt gctctttgat tttcttttct tcttcttctt tgcggcgttt
     9061 ttcttcttcg atacggcgac gcactgcttc acgttttcct tctggatctg ggtgaattgt
     9121 aacgtttcct gattcgattt cttctaaagc gcgaagagtt gatttttcag acttgaaacc
     9181 ttgagttgct ggggcacctg cttccaattc gtgggcacgt tttgcttcca agattacgag
     9241 tgaatatttt gaaggaacct tgtcgagcaa ggtatcaata gagggtttta acatcatttg
     9301 cttgtaccta ttttctaaat tttatcgggt agttggagat tttggtaaca tctcctgata
     9361 gtgaccaatg acacgatcca cacagaagtg ttctgcttca atcacacatt tgacacgttc
     9421 agcagctagg gacacctgat cgttgacaat ggcataatca tactcacgca tgagggcaat
     9481 ttcttccttg gccttttcga ttcgttgggc aatcacttct gcactatctg ttccacgacc
     9541 taccaagcga tcttgcaatt catccaaatc tggtggtgtc aggaagataa agacagcatc
     9601 tggaaccttt ttcttgacct gaagagcacc ctgaacttca atttcaagga aaacatcgat
     9661 tcccttgtcc aaggtttcat tgacataggt cagaggagtt ccatagtagt taccgacata
     9721 ttctgcgtat tccaacatct gtccttgacg aatcagctct tcaaattctt cacgagtacg
     9781 gaagaaatag tcaacaccgt ccacttctcc aggacgttgt gcgcgtgtcg tcatcgatac
     9841 agagtattga aattggtttt cagaactctc aaaaatctct cttctaaccg ttccttttcc
     9901 aacccctgaa ggaccagaaa aaacgattag taagcctcgg tctgccattg tgtctccttt
     9961 tagtcaatct gtgaaataac atttctctag aataatggca aaaagccaga ttatccttta
    10021 cagtctttct atctagtgta acaaaaaagc agtaattttt caactgctct ttcttattta
    10081 tttagcataa tctactgcac gaagctcgcg aatcacggtt accttgatat ttcctggata
    10141 atcgagattg ttttcaattt tcttacgaac tttgtgagcc aagattgtga ctttgtcgtc
    10201 cttgattttt cctggattga ccatgatacg aatttcacgt cctgcttgaa gggcaaagct
    10261 agtttgcact ccttcaaagc cgttagcaat ttcttccaaa tcatggagac gcttgatgta
    10321 gctttcaaga gactcactac gagcacctgg acgggctgcg ctcaaggcat ctgctgcagc
    10381 gacgataact gctatcacgc tctcagcttc aacatctccg tggtgactag caatcgtatt
    10441 caccacaact gggggttcct tgtacttacg ggccaattcc ataccgattt caacgtggct
    10501 accttcaacc tcatggtcaa tggctttccc gatatcgtga aggaatccag cacgacgggc
    10561 aagagccgca ttttcaccaa gttcgctcgc catgatacca gccaacttag caacctcaat
    10621 cgaatggcgc aaaacatttt gtccatatga agtacggaac tgcaaacgtc ccataatctt
    10681 catcaagtct ggatgaaggt ttggcgcacc aatttcatag gcagcagcct caccgtattc
    10741 acgaatctta ttgtcaatct cttgacggtt tttctcaacc aactcttcga tacgagctgg
    10801 atgtatacga ccatctttga gcaacatttc catagtcata cgggcaatct cacgacgaat
    10861 cggatcaaat cctgacaagg tcaccacttc tggtgtatcg tcgataatca catcgacccc
    10921 tgtcaaactt tcaaaggtac gaatgttacg accttcacga ccaataatgc gtcccttcat
    10981 agtatcgtct ggcagatgaa ctgttgagtt tgttgactcc gctacatatt caccagcgat
    11041 acgttgcata gcttgaacca agatgtcctt ggccattttg tcagaacgtt ccttgacctc
    11101 ttgctcagct tcgcgaatgc gactggcaat ctccctggtc aagttttcct ctgtctgagc
    11161 caagataata tctcgtgctt ctgcctgaga cagagcacca atacgctcta gttctgcttc
    11221 tttttgtctt tcgacttcct ctaattgctc ttcacgcgca tcaaggtttt tcgctctatc
    11281 agaaatactt tgttcttttt gttcaagtgt ttgttcttta ctcgtcaaat tgtcgtcctt
    11341 acggtcaagg ctagtagctc tctctgtcaa acgactttcg atttgtttga gttcttgacg
    11401 ttctgatttg aattcagcgt ccacttcttc acggtatttt ctggcttctt ctttggcctc
    11461 caatagtgct tcttttttaa gagacttgct ttcacgtttg gcttcattaa taagtaaatc
    11521 cgcttcacgc tcagcttgtc cacgtaaatt agttgcttct tgttcagcat ttaaaagcat
    11581 caactctgca gcttcctgag atgatttcat cttagctgag atgctgacat atccaatgac
    11641 taaaccaatg atgacggcaa aaacagcaat cgcaagcgac atgatctcca tgtttttacc
    11701 tcattttatt gttattccga atgacataca ttcttttaca ttctaccata aaaaagtgat
    11761 tttcacaaac ctaaaataga atatgttttg gggaatttgg aacacattta ccaaaataaa
    11821 cttgtttttt agaaatagta gtttagtaga gacttgagaa aaagcctact ttgcagtaga
    11881 cttcctaatg attatgaaat acaagaagtt tgtttctaac aacagcaaga tttcactcag
    11941 gtattctctg taaattagac tagtgtcctg cgcgaatttt atccattcct cgtaaaacct
    12001 tgtctgataa ctccttattt tgggcgattc tcagggtttc ctgaatactg tcccattcgc
    12061 tctttgaaag aactacaatg tcctcatctg gatttttatt gaccaccgtc aaaggctcaa
    12121 attcatcatt taccttcttc atgtagtcct ttaaatgatt tcggaatgtt gagtaaagga
    12181 ctgcttccat aaccatacct cgttttagct cttttccact attatacacg aaaagaaaga
    12241 aattgtcagg aacttgtaca agattttctt ttctatctat t
//
LOCUS       DAVAAL010000046        11960 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVAAL010000046 DAVAAL010000000
VERSION     DAVAAL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.047"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            1..683
                     /locus_tag="VQM60_001104"
                     /pseudo
     CDS             1..683
                     /locus_tag="VQM60_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(747..1829)
                     /locus_tag="VQM60_001105"
     CDS             complement(747..1829)
                     /locus_tag="VQM60_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET1080978.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     TEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(1826..2803)
                     /locus_tag="VQM60_001106"
     CDS             complement(1826..2803)
                     /locus_tag="VQM60_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET1080979.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            3018..3395
                     /locus_tag="VQM60_001107"
     CDS             3018..3395
                     /locus_tag="VQM60_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET1080980.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            3630..5021
                     /locus_tag="VQM60_001108"
     CDS             3630..5021
                     /locus_tag="VQM60_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET1080981.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            5135..5977
                     /locus_tag="VQM60_001109"
     CDS             5135..5977
                     /locus_tag="VQM60_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET1080982.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEKSVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            6250..7011
                     /locus_tag="VQM60_001110"
     CDS             6250..7011
                     /locus_tag="VQM60_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET1080983.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            7204..8121
                     /gene="glyQ"
                     /locus_tag="VQM60_001111"
     CDS             7204..8121
                     /gene="glyQ"
                     /locus_tag="VQM60_001111"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET1080984.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            8381..10417
                     /locus_tag="VQM60_001112"
     CDS             8381..10417
                     /locus_tag="VQM60_001112"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET1080985.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVHIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            10459..10716
                     /locus_tag="VQM60_001113"
     CDS             10459..10716
                     /locus_tag="VQM60_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET1080986.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(10833..>11960)
                     /locus_tag="VQM60_001114"
     CDS             complement(10833..>11960)
                     /locus_tag="VQM60_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET1080987.1"
                     /translation="EVPMFNQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISAS
                     LKSVLEWLSYEVHPFENKPVMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEF
                     LLGKAKEAFDNNGNITNEGTVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPI
                     ATTITEVDPDDPEWVEKVAAITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYAD
                     DNNQFLYYNNAHQDPDTMFAKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQ
                     EYVRTIVPGSPAGVINTHNYQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGG
                     SGPFAPAAGSHGDADATSGASDSGDAGGHGGDADATAGASY"
BASE COUNT         3640 a         2148 c         2664 g         3508 t
ORIGIN      
        1 tgatgccttc tatcaactgt tgaagcgcca tggttggcga aatattatgc cacgtccaga
       61 acatcctaag aaagcagacg ctcaaaccat tgtcgcgtct aaaaataaaa tctcaattca
      121 agaagaaaag aaagcgcttt aaaaccagta gacgttttca taaggttcgc tcgatgtacc
      181 aagatgaggc tggtttcggt agaatcagta aactgggatc ttgttggtct ccaataggag
      241 taggtccaca tgtccatagt cactatatac gagaatttcg ctattgttat ggagctgttg
      301 atgcccatac aggcgaatca tttttcttaa gagctggtgg atgtaatact gagtggatga
      361 acgccttttt agaagagctt tcacaagctt atccagatga ttatctttta ctcgttatgg
      421 acaatgctat atggcataaa tcaagtacct taaaaattcc gactaatatt ggctttgcat
      481 ttattcctcc atacacacca gagataaacc ccgttgaaca agtgtggaaa gagattcgta
      541 aacgtggatt taagaataaa gcctttcgaa ctttggaaga tgtcatgaat caacttcaag
      601 atattataca agaactggag aaggaggtga taaagtccat cgttaatcgg agatggatta
      661 gaatgctttt tgaaaacaga tgagtataaa attgaattgc ttgtaaaaaa gctccataca
      721 ctggatgtgt atagagcaat ggggctttat ttgatataga gttcttggtt ttttaggaca
      781 atttctcgga tacttgcaaa ctttttaagt tttttgattt cttctgtatg agtgacgaga
      841 gtaataacat atccttcctt gcccatacgg ccagtacggc cagcacggtg tgtgtaggtt
      901 tcgctatctc taggaatatc aaagtttacg acacattcta ggctatcgat atcaattcca
      961 cgagccaaaa ggtcagttgc aagaagcagg gttagttggt tatctttaaa cttttctaag
     1021 atgacttttc taaatttaac attaacatca ctagcgaggg aaacagccaa tatatcacga
     1081 tactgtagtt tttcctcggc attcccaagg tctgacaggc tattgaagaa gactagacca
     1141 cggaaatcct ctacatgagc cagttttcgt agtatatcca ctcgatgacg ttggtctacc
     1201 tgcatgtaga aatgctggat attgtccaat ttttgatcag agagatcaat agtgcgtgta
     1261 ttcggtacaa tcttttcttg atcaaacttg gtcgtggcac tcatgtagac cagttggtgg
     1321 tcacgaggtg cgtagtgagt gattttttct acaaagtgaa tctgagaatc atctagtaat
     1381 tggtcaaatt catccaggat gatggtttcc acattcatca tcttgatttt tttaagttta
     1441 atgagttcaa agatacggcc aggagttcca atcagaattt ctggcccctt tttaagacgt
     1501 tcaatttgtc gtttctgact tgaacctgaa aggaagagtt gagcagtcaa tccgatagct
     1561 tctgcccacg ttttacatac atcaaaaatc tgtccagcaa gttccgtatt tggtgctaga
     1621 atcaagagtt gttgggcttt tttcttttgt agtctgagaa gacttggtag gagataagct
     1681 agggtcttac cagttccggt ttggctcact cctaggaggt tttctccagc aagaaggggc
     1741 tcaaatagtt gagtttgaat gggggtgaat tcttggaaac cgagttggtc actcagttct
     1801 tgccattcag tcggtagttt ggttttcatt tttctgcctc aaatctaatg ccagcagtct
     1861 ggcgcatggt atatagtagc tcatgaacag agcctgcatc atacagccaa gtttggtaga
     1921 gattcagatc tggttgctgg atcatgtgtg caaatgcagc gacttcctca gtcatcgtat
     1981 gaggagcctg ttggatagga agctggactt gatttccttg gtggtcggta aaaatagctg
     2041 agcgaatatg ctcaatcgtg ttgagagtca aggttccatc tgttgtataa atctcgcaag
     2101 gaagattgga agtgatgttt tttccagcct tgatgtgaac ttgatagtct gggtagaaga
     2161 ggataccatc tccatttagg tcaatgctat tgtcaagctg ttgagcatgg taagtcgcgt
     2221 cattagcttt ttcaaaaaga cgaacagcag catagagggg ataaatcccc aaatccatga
     2281 gggctccacc agcaaaacgg tctgaaaaga catttggtgt ttgtccagcc aacaagtcag
     2341 gcatcttgga agagtatttg gcatagttga aatctgctcc taacacttgc ttatctgcta
     2401 aaaagttttt gatagtagta aaggctttct cgtggtaatt acgagctgct tcaaagataa
     2461 aacagttatt tttttcagct gtttgaatca aatcaaacca ttcttgtggt tgagtgacag
     2521 ctggcttttc gagaataaca tgtttaccag cagacaaggc agcttttgcc tgagcaaaat
     2581 gtaaggagtt tggactggcg atatagacta aatcaaaaga agatttgaag aagacttcta
     2641 attgatcgaa tagttggata ttctgatagc gagaagcaaa ggttgctgca gtttctagtt
     2701 ttctagaata gattgcgacc agttggtatt ctccactggt atgggctgct tctatgaaat
     2761 gatggctgat agcgccagtt ccgatgacac ctaattttag cataaatact ccttttccga
     2821 ttttaaatcc ttctttcatt ataacataga tagacgggac tatccaacag agaggagaaa
     2881 atttcaaata agctattagc tttcttttcc gaataaatag atagaagcat agaatctagc
     2941 aaacctagat ttaaaaatgt gctataatag aaggaggaaa aggaggattc tcagacatcc
     3001 aggtatcagc ccaactaatg atttgtcaat ttatccgcga tatgctggac ttgccagcaa
     3061 aaaatgtgac gattttggag ggaagtaaca ttcacgtctt gccttccatg ccctactcag
     3121 cgcaagattt ctatactagt atagacgtct tggcggagtt agataatgga atccaagtta
     3181 tcatcgaaat tcaggttcat catcagaatt ttttcatcaa tcgcctatgg gcttatctgt
     3241 gcagtcaggt taatcaaaac ctagaaaaaa ttcgccaacg tgaaggtgat acccaccaga
     3301 gctacaaaca aatcgcacca gtatacgcta tcgcaattgt cgatagtaat tacttctcag
     3361 atgacctagc ttttcatagt tttatagtaa aatgaaatga gaacaggaca aatcgatcag
     3421 gacaatcaaa tcgatttcta acaatgtttt agaagtatag gtctactatt ctagcttcaa
     3481 tctactagaa attccataga tagaaaacta cataatctct acagatacgg atgttggagt
     3541 tgatgtaaga tgctttggct tgctagagga attgtggatt gccaaattgt atcattgaaa
     3601 ttattgctca aatttgttat gatataaata tgaataaaag tagactagga cgtggcagac
     3661 acgggaaaac gagacatgta ttattggctt tgattggtat tttagcaatt tctatttgcc
     3721 tattaggcgg atttattgct tttaagatct accagcaaaa aagttttgag caaaagattg
     3781 aatcgctcaa aaaagagaaa gatgatcaat tgagtgaggg aaatcagaag gagcattttc
     3841 gtcaggggca agccgaagtg attgcctatt atcctctcca aggggagaaa gtgatttcct
     3901 ctgttaggga gttgataaat caagatgtta aggacaagct agaaagtaag gacaatcttg
     3961 ttttctacta tacagagcaa gaagagtcag gtttaaaggg agtcgttaat cgtaatgtga
     4021 ccaaacaaat ctatgattta gttgctttta aggttgaaga gactgaaaag accagtctag
     4081 gaaaggttca cttaacagaa gatggccaac cttttacact tgaccaactg ttttcagatg
     4141 ctagtaaggc taaggaacag ctgataaaag agttgacctc cttcatagag gataaaaaaa
     4201 tagagcaaga ccagagtgag cagattgtaa aaaacttctc tgaccaagac ttgtctgcat
     4261 ggaattttga ttacaaggat agtcagatta tcctttatcc aagtcctgtg gttgaaaatt
     4321 tagaagagat agccttgcca gtatctgctt tctttgatgt tatccaatct tcgtacttac
     4381 tcgaaaaaga tgcggccttg taccaatctt actttgataa gaaatatcaa aaagttgtcg
     4441 ctctaacctt tgatgatggt ccaaatccag caacgacccc gcaggtatta gagaccctag
     4501 ctaaatatga tattaaagcg actttctttg tgcttgggaa aaatgtttct gggaatgagg
     4561 acttggtgaa gaggataaaa tctgaaggtc atgttgttgg aaaccatagt tggagccatc
     4621 cgattctctc gcaactctct cttgatgaag ctaaaaagca gattactgat actgaggatg
     4681 tgctaactaa agtgctgggt tctagttcta aactcatgcg tccaccttat ggtgctatta
     4741 cagatgatat tcgcaatagc ttggatttga gctttatcat gtgggatgtg gatagtctgg
     4801 actggaagag taaaaatgaa gcatctattt tgacagaaat tcagcatcaa gtagctaacg
     4861 gctctatcgt tttgatgcat gatattcaca gtccgacagt caatgccttg ccaagggtca
     4921 ttgagtattt gaaaaatcaa ggttatacct ttgtgaccat accagagatg ctcaatactc
     4981 gcctaaaagc tcatgagctg tactatagtc gtgatgaata agcaagaaaa aataggtctg
     5041 ttagatattt gacagactta ttttttacag aagatagtac tacttaaaaa atgttttatg
     5101 ctataattga tgaataaaat agaaggagaa gcatatgaat acctatcaat taaataatgg
     5161 agtagaaatt ccagtattgg gatttggaac ttttaaggct aaggatggag aagaagccta
     5221 tcgtgcagtg ttagaagcct tgaaggctgg ttatcgtcat attgatacgg cggcgattta
     5281 tcagaatgaa aaaagtgttg gtcaagcaat caaagatagc ggagttccac gtgaagaaat
     5341 gttcgtaact accaagcttt ggaatagtca gcaaacctat gagcaaactc gtcaagcttt
     5401 ggaaaaatct atagaaaaac tgggcttgga ttatttggat ttgtatttga ttcattggcc
     5461 gaacccaaaa ccgctcagag aaaatgacgc atggaaaact cgcaatgcgg aagtttggag
     5521 agcgatggaa gacctctatc aagaagggaa aatccgtgct atcggcgtta gcaattttct
     5581 tccccatcat ttggatgcct tgcttgaaac tgcaactatc gttcctgcgg tcaatcaagt
     5641 tcgcttggcg ccaggtgtgt atcaagatca agtcgtagct tactgtcgtg aaaagggaat
     5701 tttattggaa gcttgggggc cttttggtca gggagaacta tttgatagca agcaagtgca
     5761 agaaatcgca gcaaatcacg gaaaatcgat tgctcagata gccttggcct ggagcttggc
     5821 agaaggattt ttaccacttc caaaatctgt cacaacctct cgtattcaag ctaatcttga
     5881 ttgctttgga attgaactga gtcatgagga gagagaaaac ttaaaaacga ttgctgttca
     5941 atcgggtgct ccacgagttg atgatgtgga tttctagaaa atcataaaaa gaattgtaca
     6001 ttattctaat ttttgatata atagtcagca ggaaagaaag tcttatggcg ttcttcaagc
     6061 gagcttggga tagtgggagc caagtagggc aaaataatga gctggcgctt tctgtagtat
     6121 tttcaaaaac aatgaagtaa taaattaggg tggaaccgcg tttctgacgc ccctaggtta
     6181 aatcaaccta ggattgtcgg acgcggttct tttgcttatt cagtctattg tgtgaaagaa
     6241 aggagagccg tggacaacct ttatcttgta aaagacgata gtcaactagc tacatttcgt
     6301 gattttgtag taagaaatac tgaaaagttg aaagattatc aatctttttt aaagaatgaa
     6361 cttgcagtct gtgatttacc gcaagccgtt atttggtcag attttaatgc tgctacacag
     6421 attattaggg aaagcgctgt tccagcctat acaaataata gacgaatggt tatggcgcct
     6481 gatttagctg tttggaaaga gttgtatttg tatcaattga tggactacga gtgttctcag
     6541 caaactcaag caatagaaag tcactatcat tctttatctg aaaatttcct cttacagatt
     6601 gtaggacatg agttagctca ttggtcggag cattttttag atgattttga tggttatgac
     6661 tcttatatct ggttcgaaga ggggatggtt gaatatatta gtcgcaagta tttcttgaca
     6721 gaagaggaat ttcaagcgga aaaaatttgt aatcaatctc tcgtagaact ttttcagaag
     6781 aagtatggtt ggcattcatt gaatgatttt ggttcttcga cttatgataa gaactatgca
     6841 agtatttttt atgaatactg gcgcagcttt ttgacagtag ataagttggt agaaaattta
     6901 ggtagtgtac aagcggtctt agattcttat catttatggg caaatacaga aaaaactttt
     6961 cccttgttag attggtttgt tcagcagaaa ttaattgaaa aagaaatata aaaactaaag
     7021 gagtaaacaa tgtctaagaa attgacattt cactgcgtca gtggcagaaa cctccttaca
     7081 gtcggactgc cccacgctca gcactagagt gcctgagcta gacgcagtac taactcgtct
     7141 tgcctcgtat gatcgacgag gcagactcat gtcgcaagta atttatataa aaaggagtaa
     7201 acaatgtcta agaaattgac tttccaggaa atcatcctta ctttgcaaca attttggaat
     7261 gaccaaggtt gtatgcttat gcaggcttat gataatgaaa aaggtgcggg aacaatgagt
     7321 ccttacactt tccttcgtgc tatcggacct gagccatgga atgcagctta tgtagagcca
     7381 tcacgtcgtc ctgctgacgg tcgttatggg gaaaacccta accgtctcta ccaacaccac
     7441 caattccagg tggtcatgaa gccttctcca tcaaatatcc aagaacttta ccttgaatct
     7501 ttggaaaaat tgggaatcaa tcctttggag cacgatattc gttttgttga ggacaactgg
     7561 gaaaacccat caactggttc agctggtctt ggttgggaag tttggcttga cggaatggaa
     7621 atcactcagt tcacttattt ccaacaagtc ggtggattgg caactggccc tgtgactgcg
     7681 gaagttacct atggtttgga gcgcttggct tcttacattc aagaagtaga ctctgtctat
     7741 gatatcgagt gggctgatgg tgtaaaatac ggagaaatct ttatccagcc tgagtatgag
     7801 cactcaaaat attcatttga aatttcggac caagaaatgt tgcttgaaaa ctttgataag
     7861 tttgaaaaag aagctggtcg tgcattagaa gaaggcttgg tacaccctgc ctatgactat
     7921 gttctcaaat gttcacatac ctttaatctg cttgacgcgc gtggtgccgt atctgtaaca
     7981 gagcgtgcag gctatatcgc tcgtatccgt aacttggccc gtgttgtagc caaaaccttt
     8041 gttgcagaac gcaaacgcct aggttaccca cttttggatg aagaaacacg agttaaactt
     8101 ttagcagaag acgcagaata gtgagtaata ctgtactcta aaatcgttag aggcaagtcg
     8161 aagacttgct agagggatat gcaccgccgt actgttatgt ggggattttt gaaaccttag
     8221 gttcaaaaat cagtcagcta aatccacttt gatgagattg ttggaaatct tgataaattt
     8281 agcattggat ttccatacag actcacagag ttagttagcg acgtccccag tacctaaggt
     8341 gcatatcaaa aaagattgaa gaaaatgtaa aggaaaaaac atgacaaaaa acttattagt
     8401 agaactcggt cttgaagaat taccagccta tgttgtcaca ccaagtgaaa aacaactagg
     8461 cgaaaaaatg gcagccttcc tcaaggaaaa ccgcctgtct tttgaagcca ttcaaacctt
     8521 ctcaacacca cgtcgtttgg ctgttcgtgt gactggttta tcagacaaac agtctgattt
     8581 gacagaagat ttcaagggac cagcaaagaa aattgccttg gatagtgatg gaaacttcac
     8641 caaagcagct caaggatttg tccgtgggaa aggtttgact gttgaagata tcgaattccg
     8701 tgaaatcaag ggtgaagaat atgtctatgt cactaaggaa gaagttggcc aatcagttga
     8761 agccattgtt cctggtgttg tagatgtctt gaagtcattg actttccctg tcagcatgca
     8821 ctgggctgga aatagctttg aatacatccg ccctgttcac actttaactg ttctcttgga
     8881 tgagcaagag tttgacttgg atttccttga tatcaaggga agtcgtgtga gccgtggcca
     8941 tcgtttcttg gggaaagaaa ccaaaattca gtcagcattg agctatgaag aagaccttcg
     9001 taagcagttt gtaatcgcag atccatgtga acgtgagcaa atgattgttg accaaatcaa
     9061 ggaaattgag gcaaaacatg gtgtacatat cgaaattgat gcggatttgc tgaatgaagt
     9121 cttgaatttg gttgaatacc caactgcctt catgggaagt tttgatgcta aataccttga
     9181 agttccagaa gaagtcttgg tgacttctat gaaggaacac cagcgttact ttgttgttcg
     9241 tgatcaagat ggaaaactct tgccaaactt catttctgtt cgtaacggaa acgcagagcg
     9301 tttgaaaaat gtcatcaaag gaaatgaaaa agtcttggta gcccgcttgg aagacggaga
     9361 attcttctgg cgtgaagacc aaaaattggt gatttcagat cttgttgaaa aattaaacaa
     9421 tgtcaccttc catgagaaga ttggttccct tcgtgaacac atgattcgta cgggtcaaat
     9481 cactgtactt ttggcagaaa aagctggttt gtcagtggat gaaacagttg accttgctcg
     9541 tgcagcagcc atttacaagt ttgacttgtt gacaggtatg gttggtgaat ttgacgaact
     9601 ccaaggaatt atgggtgaaa aatacaccct tcttgctggt gaaactccag cggtggcagc
     9661 tgctattcgt gaacactaca tgcctacatc agctgaagga gaacttccag agagcaaggt
     9721 cggcgcagtt ctagccattg cagacaaatt ggatacgatt ttgagtttct tctcagtagg
     9781 attgattcca tcaggttcta atgaccctta tgcccttcgt cgtgcaactc aaggtgtggt
     9841 tcgtatcttg gatgcctttg gttggcacat tgctatggat gagctgattg atagccttta
     9901 tgcattgaaa tttgacagtt tgacttatga aaataaagca gaggttatgg actttatcaa
     9961 ggctcgtgtt gataagatga tgggctctac tccaaaagat atcaaagaag cagttcttgc
    10021 aggttcaaac tttgttgtgg cagatatgtt ggaagcagca agtgctctcg tagaagtaag
    10081 caaggaagaa gattttaaac catctgttga atcactttct cgtgccttta acctggctga
    10141 gaaggcagaa ggggttgcta cggttgattc agcactattt gagaatgacc aagaaaaagc
    10201 tttggcagaa gcagtagaaa cactcgtttt atcaggacct gcaagtcagc aattgaaaca
    10261 actctttgcg cttagcccag tcattgatgc tttctttgaa aatactatgg taatggctga
    10321 agatcaggct gtccgtcaaa atcgtttggc aatcttgtca caactaacca agaaagcagc
    10381 taagtttgct tgttttaacc aaattaacac taaataaaat ttgataaacg gactttatct
    10441 tattacaaag gagaagaaat ggatccgaaa aaaattgctc gtatcaatga gcttgctaaa
    10501 aagaaaaaaa cagaaggctt aacaccagaa gaaaaagtgg aacaagccaa actacgtgag
    10561 gagtacatcg aaggttatcg ccgcgctgtt cgtcaccaca ttgaaggaat caaaattgtg
    10621 gacgaagaag gaaacgatgt tacaccagaa aaactacgcc aagtacaacg tgaaaaagga
    10681 ttacatggcc gtagtcttga tgatccaaat tcataatatg tattctttct tttaacaaag
    10741 atagatgaaa cgataacaaa gagactagca gtttgtgttt gctagtcttt tttcgctaaa
    10801 aaaggaacca taatggttcc taaaaactat cattagtaac ttgcaccggc tgtagcgtct
    10861 gcgtcaccac cgtggcctcc agcatcccct gaatcagaag cgccagaagt agcatcggcg
    10921 tctccatgac ttccggcagc aggagcaaat ggtccgctac cacccaccaa acgttgacca
    10981 gtctctttta ggtaccagtc aagccatggt tggaagttaa agacgatttc attgatacca
    11041 gcgtatgatc catcaggata gtacattgct tggtagttgt gagtgttgat aacacctgca
    11101 ggagaacctg gaacgatcgt acggacgtat tcttggtttc cgttgcgaag tgttccgata
    11161 acccactcta cgttcttcat acgtgctggt ggaagagaac catgaacagt cgacatacgg
    11221 ctacctgatt gaggtggtac acgtttagcg aacatagtgt ctggatcttg gtgagcgttg
    11281 ttgtagtaga ggaattggtt gttgtcgtca gcgtatgtca attcaaatgg catagctttc
    11341 aagaacatat caatttggtt aactgttagg ataccgtggt ccaatttgac ataggtatca
    11401 ccagaaacag caccagtgat tgctgcaact ttttctaccc attctggatc gtcagggtca
    11461 acttctgtga tggttgtagc gattggtttt ccacaatcca agtcttctga ttcgattggt
    11521 tttggttttt tcaatttcga aacgactcct acgtatttaa caaagttatc taagcaagtt
    11581 tcaaggaatt taacagtgcc ttcgttggtg atatttccgt tgttatcaaa agcttcctta
    11641 gctttaccaa gaaggaattc gttacctgga agcgtgtagg cattaacacc tggagcatca
    11701 aggattttac gaaggtgaac ttgagcacgt gatgttcctt ggtcatagta tgatgcaccc
    11761 acaatcataa caggcttgtt ttcaaatgga tgaacttcgt atgaaagcca ttcaagtaca
    11821 gatttgagtg aagctgagat agtgtggttg tgctcaggag tggcgataat gacaccatca
    11881 gcacgtgtaa tcttgttata caagaaacgc aattggaagc tttcgtccca tttttcgtct
    11941 tggttaaaca ttggaacttc
//
LOCUS       DAVAAL010000047        11851 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVAAL010000047 DAVAAL010000000
VERSION     DAVAAL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.048"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..418
                     /locus_tag="VQM60_001115"
     CDS             <1..418
                     /locus_tag="VQM60_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET1080988.1"
                     /translation="LSQAHFQANGLSTDEHRFIVMDVFEYFKYAKRKDLTYDVIVLDP
                     PSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAANVSRQKFTEQIDKGFAG
                     RSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            415..1092
                     /locus_tag="VQM60_001116"
     CDS             415..1092
                     /locus_tag="VQM60_001116"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET1080989.1"
                     /translation="MKLIVSVMPRSLEEAQALGATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            1082..1936
                     /locus_tag="VQM60_001117"
     CDS             1082..1936
                     /locus_tag="VQM60_001117"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET1080990.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLYALENVSELQARIVESDLLVNATSVGMDGQSSPVPESINL
                     PETILVADIIYQPFETPFLKWARSQGKPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            1955..3022
                     /locus_tag="VQM60_001118"
     CDS             1955..3022
                     /locus_tag="VQM60_001118"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET1080991.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCLAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTELDGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGVRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEKKGLMPAGITQSITDMCQKFGLPVDYENWDVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            3032..4198
                     /gene="aroC"
                     /locus_tag="VQM60_001119"
     CDS             3032..4198
                     /gene="aroC"
                     /locus_tag="VQM60_001119"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET1080992.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINGDLKRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVVNKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKYRDYTKNY"
     gene            4208..5311
                     /locus_tag="VQM60_001120"
     CDS             4208..5311
                     /locus_tag="VQM60_001120"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET1080993.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            5322..5660
                     /locus_tag="VQM60_001121"
     CDS             5322..5660
                     /locus_tag="VQM60_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET1080994.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAHTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            5752..7035
                     /gene="aroA"
                     /locus_tag="VQM60_001122"
     CDS             5752..7035
                     /gene="aroA"
                     /locus_tag="VQM60_001122"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET1080995.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGASVIIEKECTRNHTEDMLKQFGGHLSVDGKKIIVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            7028..7504
                     /locus_tag="VQM60_001123"
     CDS             7028..7504
                     /locus_tag="VQM60_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET1080996.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDTNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            7501..8349
                     /gene="pheA"
                     /locus_tag="VQM60_001124"
     CDS             7501..8349
                     /gene="pheA"
                     /locus_tag="VQM60_001124"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET1080997.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSTEEYGLELIA
                     EDIQEMEANLTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            8346..9620
                     /gene="psr"
                     /locus_tag="VQM60_001125"
     CDS             8346..9620
                     /gene="psr"
                     /locus_tag="VQM60_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET1080998.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            9899..10744
                     /locus_tag="VQM60_001126"
     CDS             9899..10744
                     /locus_tag="VQM60_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET1080999.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKNFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDILPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDTVIADMDKSYTKYK
                     GEYGG"
     gene            10734..11837
                     /locus_tag="VQM60_001127"
     CDS             10734..11837
                     /locus_tag="VQM60_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET1081000.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTRNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
BASE COUNT         3498 a         2220 c         2814 g         3319 t
ORIGIN      
        1 attgtctcaa gcgcattttc aggcaaatgg gctcagcaca gacgagcatc gttttatagt
       61 catggatgtc tttgagtatt tcaaatatgc caaacgcaaa gacttgacct acgatgtgat
      121 tgtcctagat ccgcctagct ttgctcggaa taaaaaacaa actttctctg tggccaagga
      181 ttatcacaag ttgatttccc agagtcttga gattttaaat ccgggaggga ttatcattgc
      241 cagtaccaat gctgccaatg tttcccgtca gaaatttaca gaacaaattg ataaaggctt
      301 tgcaggaaga agttaccaga ttttaaacaa atatggtctt ccagcagatt ttgcctataa
      361 taaaaaagat gaaagtagta attacctcaa ggtgattagt atgaaggtta gtaaatgaaa
      421 ttaatcgttt cagtaatgcc aagaagttta gaggaggctc aggctctggg tgccacgagg
      481 tacctggatg ccgacatcat tgaatggcgt gccgactatc tgcctaaaga agcgattttg
      541 caggtagctc cagccatttt tgaaaaattc gcaggccgtg agttggtttt cacgctacga
      601 actcgctccg aaggtggaga aatcgacctt tctccagaag agtatatcca tctaatcaag
      661 gaagttgcgc aattctatca accagactat attgattttg agtactatag ctacaaggat
      721 gtttttgagg aaatgctgga cttcccaaat cttgttttga gttaccataa tttccaagaa
      781 acacctgaga acatgatgga aatcttgtca gagttgacga tcctaaatcc aaaacttgtt
      841 aaggttgcgg tgatggctca cacggagcag gatgtcttag acttgatgaa ctatacacga
      901 ggctttaaaa ccctcaatcc tgaacaggaa tatgtgacca tttctatggg taaggtgggc
      961 aaggtctctc gtatcactgc ggatgtgact ggttcgagtt ggtcttttgc cagtctggat
     1021 gaggttagtg cccctgggca gatttctcta gctagcatga aaaaaatcag ggagattttg
     1081 gatgaagctt gatggctata cacgtttagc tgcagttgtt gccaatccta ttaagcattc
     1141 tatttctccc ttcatccaca atagagcctt tgaggcgaca gctaccaacg gtgcttatgt
     1201 ggcttgggag attgaagcga gtgacttggc agaaacagtg gccaatattc gtcgctacca
     1261 gatgtttggc atcaatctgt ccatgcccta taaggagcag gtgattcctt atttggataa
     1321 gctgagcgat gaagcgcgct tgattggtgc ggttaatacg gttgtcaatg agaatggcaa
     1381 tttaattgga tataatacag atggcaaggg attttttaag tgcttgcctt cttttacaat
     1441 ttcaggtaaa aagatgaccc tgctgggtgc aggtggtgcg gctaaatcaa tcttggcaca
     1501 ggctattttg gatggcgtca gtcagatttc ggtctttgtt cgttcagttt ctatggaaaa
     1561 aacaagacct tacctagaca agttacagga gcaaacaggc tttaaagtgg acttgtatgc
     1621 tttagaaaat gtttctgaac tgcaagcaag gattgtcgag tcggatttac tggtcaatgc
     1681 caccagtgtg ggcatggatg gtcaatcatc cccagttcct gaaagcatca atttgccaga
     1741 aactatcttg gtggcagata ttatttatca accctttgaa acgccatttt taaaatgggc
     1801 cagaagtcag ggcaaaccag cggttaatgg tctgggaatg ttgctctatc aagctgcaga
     1861 agcttttcaa ctgtggacag gcaaggaaat gccaacagaa gagatttggc agtctttaac
     1921 agaaaaatac caataaagaa aaggagattc tcctatgaaa atcagaatcg atattcctca
     1981 tcatccttat gatattcaga ttgaaaaagg ttgtctggct caggctggtc aatggttgcg
     2041 agaactctgg caaccacaaa aggtagtcat tgtgacagat aaccatgtag cttctcttta
     2101 tgcagagaag gtcaagctca gcctagaaga tgctggtttt caggtagctg tttttgactt
     2161 tttagaaggg gaagaaagaa agaatttaac cactgttcag aaagtctatg aattcctagt
     2221 taagcagggg ttgactcgta gcgatggaat cgtggctctc ggtggtggtg tcgttgggga
     2281 cttggctggt tttgtagcct ctacctatat gcggggcatt cattttgttc agataccgac
     2341 tagcttaaca gcccaggtgg attcttctat tggtggaaag acgggtgtta acactccatt
     2401 tgccaagaat atggtaggaa cttttgccca accagatggg gttttgattg acccacttgt
     2461 tcttgaaacg cttggaaaaa gagagttgat tgaagggatg ggtgaggtta tcaagtatgg
     2521 cttgattgag gatccagaac tgtgggctct tttgacggag ctggatggtt ctgttgagag
     2581 cattctggaa catgcagaga ccttgattga acattcttgt caggttaagc gcaagatggt
     2641 ggttgaggat gagttggaca atggtgtacg cctttacctc aactttggtc acactattgg
     2701 tcatgctatt gaagcaactg ccggttatgg taaggtcatg catggtgagg ctgtggccat
     2761 gggcatggtg cagatttcca aggttgctga gaaaaaaggc ctcatgccag ctggcataac
     2821 tcagtccatt acagatatgt gtcagaaatt tggcttgcct gttgactatg aaaactggga
     2881 tgttgacaag ctttatcagg ctttgactca tgacaaaaaa gcgcgtggca ataccttgaa
     2941 attggtcttg gtaccagagc ttggttcagc gaccattcac ccagtttctc tggaagagat
     3001 gaaagactac ttggtaaaat aaggagagtg tatgagatat ttaactgcag gagaatcaca
     3061 cggtccccgt ctgacagcta tcattgaagg aattccagct ggactacctt tgacagctga
     3121 ggatatcaat ggcgacctta aacgccgtca gggtggctac ggtcgtggcg gtcgtatgaa
     3181 gattgagagc gaccaagttg tctttacttc aggtgttcgc catgggaaga cgacaggggc
     3241 tcctattacc atggatgtcg tcaataagga ccaccaaaaa tggctggata tcatgtctgc
     3301 agaggacatt gaagaccgcc ttaaaagcaa acgaaaaatc acccatcctc gcccaggaca
     3361 tgccgatttg gttgggggca tcaagtaccg ttttgatgat ttgcgaaatt ctttggagcg
     3421 ttcatcagct cgtgaaacca ccatgcgggt ggcagttggt gcagtagcca aacgcctctt
     3481 ggctgagctg gatatggaga ttgccaacca tgtcgtggtc tttggtggca aggaaatcga
     3541 tgttcctgaa aatctgacag tcgctgaaat taagcaaaga gctgcccagt ctgaagtttc
     3601 tattgttaac caagaacgag aacaggaaat caaggactat attgaccaaa tcaaacgtga
     3661 tggtgatacc atcggtgggg ttgtggagac agtcgtcgga ggcgttccag ttggtcttgg
     3721 ttcctatgtc caatgggata gaaaattgga tgcaagattg gctcaagctg ttgtctctat
     3781 caatgccttt aaaggggtgg aatttggtct tggctttgag gctggttatc gtaaaggcag
     3841 ccaagttatg gatgaaattc tctggtctaa agaagacggt tatactcgcc gtaccaataa
     3901 tctaggtggt tttgaaggtg gtatgactaa tgggcaaccc atcgttgttc gtggggtcat
     3961 gaaacccatt cctactcttt ataaacctct tatgagtgtg gatatcgaaa cccacgaacc
     4021 ttacaaggca accgtggaga gaagtgatcc gactgctctt ccagctgcag gaatggtcat
     4081 ggaagcagtt gtagcaacgg ttctggcgca agaaatcctc gaaaaattct catcagataa
     4141 tcttgaggaa ctaaaagaag cggtagccaa ataccgagac tatacaaaga actattaagg
     4201 agttcctatg gcaaaaacaa tctatatcgc aggtcttggg ttgattggag cctctatggc
     4261 acttggtatc aaacgcgatc atccagatta tgaaatttta ggttataatc gtagtcaagc
     4321 ttcgagagat atcgccttga aagaaggcat gattgaccgt gcaacggatg attttgctag
     4381 ttttgctcct ttggcagatg tcattatcct cagcttgcca atcaaacaaa ctattgcttt
     4441 cattaaggag ttggccaatt tggatttgcg agaaggcgtt attatttcag atgctggttc
     4501 gaccaagtca accattgtgg atgcggcgga gcagtatttg gctggcaagt ctgttcgctt
     4561 tgtcggggcc catcccatgg ctggtagtca caagacaggg gctgcttcgg cagatgtcaa
     4621 tctttttgaa aatgcctatt atatctttac accttcaagc ctgacaagtc aggacacgct
     4681 taaggaaatg aaggatctgc tttcaggtct tcatgctcgt tttatcgaga ttgatgccaa
     4741 ggagcatgat cgtgtcactt ctcagattag ccattttcct catattttgg cttctagtct
     4801 catggagcag actgcggtct atgctcaaga gcatgagatg gcaaggcgct ttgcggcagg
     4861 tggttttcga gatatgaccc gaattgcgga aagcgagcca ggaatgtgga cctccattct
     4921 cttgtccaat agcgagacca ttctggatag aattgaggat ttcaaggaac gtttggaagt
     4981 gattggtcag gccattagta agggagatga agagcaaatt tggaactttt ttaaccaagc
     5041 gcgtgaacaa cgccagacca tggaaatcca taagcgtggt ggtgtggata gctcttacga
     5101 cctctatgtt gacgttcctg atgaagaaga tgtcatcctg cggattttgg aactgctacg
     5161 tgggacttcc ttggttaaca tccacatcaa cgaggaaaat cgtgaggata ttcacggaat
     5221 cctacaaatt tcatttaaaa atgctcaaga cttggaaaga gctgagcatc tcataacaga
     5281 aaataccgac tatacagtcg tcatcaaata aggagaagaa catgtcaaat atttacgata
     5341 gtgcaaacga actcagtcgc ggtctacgcg gattaccaga atacaaggct gttaaagcag
     5401 ctaaagatgc gattgcagca gatgctgagg caagcaaaat ctttacagaa tatcttgcct
     5461 ttcaagagga aattcaaaaa ctagcacata caggtcaaat gccagatgcg tccttccaag
     5521 ctaagatgga aggctttggt aaacagattc aaggaaatag cctcttgtca gaattcttta
     5581 ccaagcaaca acaattggcc atttaccttt ctgacattga aaaaattgtt tttgaaccag
     5641 tttcagaatt gctgaaataa gaaaagaacg atcctaaagt caggaatttt taaggaattt
     5701 ctggcttttt atgataaaat aggtaaaagt attgcaagta tgaggtccag tatgaaacta
     5761 aaaacaaaca ttcgccattt acatggtagt atccgcgtcc caggtgacaa gtctatcagc
     5821 caccgttcca ttatctttgg aagtttggct gagggtgaga ccaaggttta tgatattctg
     5881 cgaggtgaag acgtcctttc gaccatgcag gtttttcgtg accttggtgt tgaaattgag
     5941 gataaagatg gggttattac cattcaaggt gtaggcatgg ctggcttaaa agcgccacaa
     6001 aatgccctta atatgggaaa ttctggcacc tcgattcgcc tgatttcagg tgtccttgct
     6061 ggtgcagatt tcgaagtaga gatgtttgga gatgatagtc tttccaaacg tcctatggac
     6121 cgtgtgaccc ttccactgaa aaaaatgggc gtcagcattt caggacaaac tgaacgagac
     6181 ttgcctcccc ttcgcttaaa agggacgaaa aacctaagac ctattcatta tgagttgcca
     6241 attgcctctg cccaagtcaa gtcagccttg atgtttgcag ccttacaagc taagggggcg
     6301 tcagttatta tcgaaaaaga gtgcactcgt aatcatactg aagatatgtt gaaacaattt
     6361 ggtggtcatt taagtgtgga cggtaagaaa atcatagtcc aaggaccaca aaaattgaca
     6421 ggacagaagg tggtcgtacc aggagatatt tccagtgcag ccttttggtt agtcgcaggt
     6481 ttgattgttc caaattctcg tctagtgctg cagaatgtgg ggatcaacga aactcgcacc
     6541 ggtattattg atgtcattcg tgccatgggt ggaaaattgg aaataactga aatcgatcca
     6601 gtcgctaaat ctgcaacctt gattgttgag tcttctgact tgaaaggaac agagatttgt
     6661 ggcgctttga ttccacgttt gattgatgaa ttgcctatta ttgccctact tgcgacccaa
     6721 gcccaaggtg taacagttat caaggatgct gaggagctca aggtcaagga aacagaccgt
     6781 attcaggttg tggcagacgc cttaaatagt atgggagcag atattactcc tacggcagat
     6841 gggatgatta tcaaaggaaa atcagctctt cacggtgcta gagtcaatac gtttggtgac
     6901 caccgtatcg gcatgatgac agctatcgca gccctcttgg ttgcagatgg agaggtggag
     6961 cttgaccgtg cagaagccat caataccagc tatcctagtt tctttgatga tttggagagc
     7021 ttgattcatg gctaaggtgt tattagggtt tatgggggct ggaaaatcga ctattgcaag
     7081 aggcttggac actaattacc ttgatatgga tgctctgatt gagaagcgcc taggtatgtc
     7141 cattgcgaat tttttcgctg aaaagggaga agcagccttt cgtcatgtag aatcagaagt
     7201 cctagctgat ttactacaaa cagaccaagt cgtgtcaact ggaggaggag tggttatttc
     7261 tcagagaaat cgtgacttac tcaagactaa tacagataac atctacctga aagcagattt
     7321 tgaaaccctc tacctacgta tcgcagctga taaggacaat cagcgaccgc tttttctaaa
     7381 taatagcaag gaagaactag tagctatttt tcaagaaaga caggcttggt atgaggaagt
     7441 ggctagtcgg gttttggatg tgaccaagct aagcccagag gaaattatag aggaactaag
     7501 atgaaaattg cttatctagg tcccaaggga tcattttcac accacgttgt gcagacagct
     7561 tttcctcatg aggaattgca ggcttttgcc aacattacag atgtcatcaa ggcttatgag
     7621 caaggattag tggactattc tgtggtgcca gttgaaaact ctattgaggg tagtgttcat
     7681 gaaaccttgg actatctttt tcatcaggct cacatccaag cagtagcaga aatcgttcag
     7741 cctattcatc agcagttgat ggtggttcca ggccatacta agattgaaaa gattttttca
     7801 catccacagg ccttggctca aggaaagaaa ttcatcgatg aacaatatcc agaggctcaa
     7861 atcgaggtaa cagctagtac agcttatgcg gcccgtttta tttccgaaca tccagatcag
     7921 ccctttgcag caattgcacc tagaagttct actgaagaat atggattgga actgattgcc
     7981 gaggatattc aggaaatgga agccaattta acacgtttct gggttctagg agctgaaaag
     8041 cctagtattc ccttgcaagc acaaactgaa aagatgagtt tggccttgac attacctgac
     8101 aaccttccag gtgcacttta taaggccctg tcgacctttg cttggcgagg aattgacttg
     8161 acaaaaattg aaagtcgtcc actcaagaca gcactgggtg aatacttttt cattatcgat
     8221 gtggattata ccgataagga cttggtccac tttgcccaaa aagaattaga agcgattgga
     8281 atccagtata aaattctggg tgcctatcct atttatccaa tatcagacca tggaaaggag
     8341 agaagatgag taaagaaaat cccttaagtc atcatgagca gttgcgttat gattatttgc
     8401 taaaaaatat tcactatctc aatgagagag aaaaaaatga gtttgcctat ttgcaagaaa
     8461 agctaactct tgctagggga aatagtagct ctagcttgga acaagaaaga gaagagcagg
     8521 ttgacttacc aagctatgcg aaccggagtc gctcacaatc caaatcacaa gcactctctt
     8581 tccctccaaa aaagaaaaga cggaagctcc gtcttaaacg aatttttatg gtgatttttt
     8641 cacttttggt ctgtgtggct ttggccatgg tattcatgtt tttgcgtggt taccaagatg
     8701 ctagcgcaaa gaaaactgct gatgcccggg cagctcaagt agaagtcttt aatggtcagg
     8761 acactagaga tggagttaat attttaatca tgggtactga tggtcgaatc ggccagaaca
     8821 gtgttgagac acgaactgac tctattatgg tattaaatgt cgggggctca gataagaaaa
     8881 tgaagctagt cagtttcatg cgtgataatt tggtctatat agatggttat agtcaagtga
     8941 ttaacggtag aaaacagaca gataacaagt taaacgtagc ctacgagtta ggagaacaag
     9001 aggggcaaaa aggggcagaa atggttcgcc aagtcttgaa agataatttt gacttggaca
     9061 ttaagtacta cgccttggtt gatttccagg cctttgccac agcgattgac acgcttttcc
     9121 ctgatggggt gacgattgat gctcaatttt caacattgaa tgggcgtccc ctaacagaag
     9181 ctacagtcgg agatgattta tacgctaccg agactgagtc tccaacccaa accattaaag
     9241 tcggaaaaca gcagatgaat ggctcgaccc tgctcaatta tgctcgtttc cgtgatgatg
     9301 atgaggcgga ttacggccgt acaaaaagac agcaacaagt tttaacagca attttagagc
     9361 aaattaaaga tcccactaaa cttttcactg gttctgaagc tcttggaaag gtttttgcta
     9421 tgacctcaac caacgtcccc tatactttcc tcctaacaaa cggtttatct gttttggatg
     9481 gagcaaaaaa tggtattgaa aaattgacga ttccagaact gggtgactgg gtagatgcct
     9541 atgatgttta tggaggcttg ggcttgctgg ttgatcaaaa caaatatcaa accaagctcg
     9601 ctcaaatggg cttgagataa gataatactc aatgaaaatt aaagtgcaaa ctaggaagct
     9661 agccgcaggc tgctaaaaac acagttttga ggttgtagat aagactgacg aagtcagtta
     9721 cctataccta cggcaaggtg aagctgacgt ggtttgaaga gattttcgaa gagtataaga
     9781 cagaaaaatc aaagtttgat agctatttgg atagcagtca ggctttgatt ttttacagtc
     9841 tgcaatagat tttcacccct tgtttgtggt ataatgaaac gatacgatag aaagaataat
     9901 gaacaatatg actgatttaa aagcaattca ggctcgtagt ctggagatgg ctgaatattt
     9961 tgtggccttt tgtaaagaac atgatttgct ctgttatctc tgtggcggag gcgctattgg
    10021 tgcccttcga aacaagggat ttattccttg ggacgacgac ctagactttt ttatgcctcg
    10081 taaagattat gagaaattag cagaattatg gcctcgttat gcagatgaac gttatttctt
    10141 gtcaaagagt cataagaatt ttgttgatcg taatcttttt attaccattc gtgacaagga
    10201 aaccacctgt atcaagcctt atcagcagga tttggatttg ccacatggtc tggccttgga
    10261 tattttgcct ttggattatt atccgaaaaa tccagctgag cggaaaaaac aggttcgttg
    10321 ggccttgatt tattcactct tttgtgcgca aactattcca gaaaagcatg gtgctctcat
    10381 gaaatgggga agtcgcattt tactgggttt gactccaaaa tctctccgtt atcgcatctg
    10441 gaaaaaagct gagaaagaaa tgactaagta tgatttggct gattgtgatg gcattacaga
    10501 attatgctca ggtcctggct acatgagaaa caagtaccca atcacatctt ttgaagacaa
    10561 tcttttcttg ccatttgaag gaacagagat gcctattcca atcggctatg atgtctatct
    10621 cagaactgct tttggggatt atatgacgcc tccaccagca gacaagcagg taccgcatca
    10681 tgatactgtc attgctgata tggataagtc ttatacaaaa tacaagggag aatatggtgg
    10741 ctaagaaaaa aatcttattt tttatgtggt ctttttctct tggaggtggt gcagagaaga
    10801 ttctatcaac cattgtttca aatctggatc cagaaaagta tgatattgat attcttgaaa
    10861 tggagcactt tgacaaggga tatgaatctg ttccaaagca tgtacgcatt ttaaaatccc
    10921 ttcaagatta tcgccaaacc agatggttac gagctttttt gtggagaatg agaatttatt
    10981 ttccaagact gactcgtcgt ttgcttgtaa aagatgatta tgatgttgaa gtttctttta
    11041 ccattatgaa tccaccactg ttgttctcta aaagaagaga agtcaagaag atatcttgga
    11101 ttcatggaag tattgaagaa cttcttaagg atagctctaa aagagaatca catagaagcc
    11161 agttggatgc tgcgaataca attgtaggga tttcaaaaaa gaccaggaat tctatcaagg
    11221 aagtttatcc agattatgct tctaaattac agacaatcta caatggatat gattttcaga
    11281 ctattctaga aaaatctcaa gagaagatcg atatcgagat tgctcctcaa agtatctgta
    11341 ctatcggacg gattgaggaa aataagggtt ctgaccgtgt agtggaagtg atacgattat
    11401 tacaccaaga gggaaaaaac tatcatctct attttatcgg ggctggtgat atggaagagg
    11461 aactgaaaaa acgagtcaaa gagtatggga ttgaggacta tgtacatttc cttggttatc
    11521 aaaaaaatcc ttatcagtat ctatctcaga cgaaagttct cttgtctatg tctaaacaag
    11581 aaggttttcc tggagtgtat gtggaggcct tgagtctggg actccctttt atctctacgg
    11641 acgttggagg ggctgaggaa ttatcccaag aaggacgatt tggacaaatc attgagagca
    11701 atcaagaggc agctcaggcg attactaatt acatgacttc tgcctcaaac tttgatgtcg
    11761 atgaggctag ccaattcatt caacaattta caattacaaa acaaatcgaa caagtagaaa
    11821 aactattaga ggagtagcat ggaaactgca t
//
LOCUS       DAVAAL010000048        11712 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVAAL010000048 DAVAAL010000000
VERSION     DAVAAL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.049"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(147..950)
                     /locus_tag="VQM60_001128"
     CDS             complement(147..950)
                     /locus_tag="VQM60_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET1081001.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLFSEKMYLDLDLDFS"
     gene            complement(1553..2932)
                     /locus_tag="VQM60_001129"
     CDS             complement(1553..2932)
                     /locus_tag="VQM60_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET1081002.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNVKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEII
                     SEIMDESYTDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVND
                     IVNAQSNSLILIDEPEISLHPSAIYKFKEFLLQECLNKKHQIIITTHSTQLIKDFPRE
                     AVKLLVKNGEKVDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSE
                     NLKQNLVVRYIPGGANQIICNNILNSSYLDSDNHYFWLDGDQNTNVSESNNLMNYLEN
                     GVVISDKIPESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKY
                     VSYLPFPTP"
     gene            complement(2983..3147)
                     /locus_tag="VQM60_001130"
     CDS             complement(2983..3147)
                     /locus_tag="VQM60_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HET1081003.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            complement(3228..5879)
                     /locus_tag="VQM60_001131"
     CDS             complement(3228..5879)
                     /locus_tag="VQM60_001131"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET1081004.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVVHDNVTKVATGEAGNVTDRWILHNLNETIGKA
                     TANFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ECDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(5872..6450)
                     /locus_tag="VQM60_001132"
     CDS             complement(5872..6450)
                     /locus_tag="VQM60_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET1081005.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQHQPNELNH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(6450..7010)
                     /locus_tag="VQM60_001133"
     CDS             complement(6450..7010)
                     /locus_tag="VQM60_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081006.1"
                     /translation="MARTELPDKIETECLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYAFLKSEWEVI"
     gene            complement(6997..7446)
                     /locus_tag="VQM60_001134"
     CDS             complement(6997..7446)
                     /locus_tag="VQM60_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HET1081007.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVVPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(7859..8254)
                     /locus_tag="VQM60_001135"
     CDS             complement(7859..8254)
                     /locus_tag="VQM60_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET1081008.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(8350..8604)
                     /locus_tag="VQM60_001136"
     CDS             complement(8350..8604)
                     /locus_tag="VQM60_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET1081009.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(8614..9948)
                     /locus_tag="VQM60_001137"
     CDS             complement(8614..9948)
                     /locus_tag="VQM60_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET1081010.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(9948..10181)
                     /locus_tag="VQM60_001138"
     CDS             complement(9948..10181)
                     /locus_tag="VQM60_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET1081011.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            complement(10494..10646)
                     /locus_tag="VQM60_001139"
     CDS             complement(10494..10646)
                     /locus_tag="VQM60_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081012.1"
                     /translation="MKFFWGLLAIIFIKPIIGIVKFFWMIISFAVQLLFYKIVFKILD
                     WLFKLI"
     gene            complement(10643..10825)
                     /locus_tag="VQM60_001140"
     CDS             complement(10643..10825)
                     /locus_tag="VQM60_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081013.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEDKK"
     gene            complement(10827..11606)
                     /locus_tag="VQM60_001141"
     CDS             complement(10827..11606)
                     /locus_tag="VQM60_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081014.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGPTTLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEALWTSREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT         3427 a         2532 c         2111 g         3642 t
ORIGIN      
        1 tgtgcaatac agaactaaat ccttcggata aattatttgt ctaacttttt ggggtcagta
       61 cacctaaaac tttgatgata tacgttttct tgtgagaata tttacttcat ttttgcctaa
      121 aattcaatgt ttactcagta tttggattat gaaaaatcga ggtctaaatc tagatacatt
      181 ttttctgaaa acaaatcatt ttgaccaccg agcaagagat tttcaaaaaa agctgttaaa
      241 aactcagaac gtcgctgtaa aatctttgca ttatctaata ccaaggcatc acgaaaatat
      301 ttggaatgtt gctgaaatgg tgtattatca atatcaaaac caaactcacg aagatactga
      361 atcaaaaaga ccgttactgt ccgagtgttt ccttcgcgaa atggatgaat ctgccagatt
      421 cctgaaataa aatgctggat ttgtttaacc acatccgcct gagttagtgt cgcatatgca
      481 acttgttttt cctgattaaa atcataatct aaggtcattt gaatcatgga gtaatcagag
      541 tacacaacac tttcaccatt caaaacaggt tcattctttg tgatattggt ctgacgaaat
      601 tgccccaccg gaatagaggg ttcaaatata tcttgaaaca actccttatg aatagcaagt
      661 aaggtcgcag gactaaagct aaagcctctt cgagacaata gttctacaat gcgtagagaa
      721 accaagtctg cctcttccgt acttgcatca atagtatggt gataagccgt tgcatcctca
      781 taaacctgct cataagtcag ttctccccgg gactgtttct cagccaaaga ttccatatac
      841 gctgatggca ctagattgtc aactttctgc agaccaaaac ctatccgcca taaatcacgc
      901 ttcgcttcat aagacaagtt tggattgtca atgttgtaag ttgattgcat agaaatatcc
      961 tttcaattgt ttttctgtgt catcggtaaa ttgtgaaata ggtatggaat tggcgaaaat
     1021 tataaaaatc cttcacaata ggttctatga aatatatata cttcatctta atagtgagca
     1081 agctaaactt agcatttcat gccctcatat gggatgttct ttaactaaat aatatgatta
     1141 tcgagatata tctggataaa tgaactaata agtctgacgc gtagacttat caaagtcatt
     1201 ggcatacacc actatgaact cgttgatctg ttcaaatccc aacacattac ctgagaagaa
     1261 agttgcaatg ttgtttttgg tgcgggtttg aatttaaaaa tttgttatgt agtacctaat
     1321 ctaaggaatt agaacaatgc ctctaatttt tctttaatac actgaaacat tgatgattct
     1381 ggctgtattt ttgaaacagc tcttctttgc tcctggaaaa tatcttcaga agttatattc
     1441 tctattccta acgctacttg aatttttttc taaaatattc ttttccgttg ccatctttag
     1501 aaaaatcata accttcccta tctacgctgt tacataaatt agctaaaaaa aactatgggg
     1561 ttgggaaagg aagataagaa acgtatttag cccaataatc tataaagctt ctttgtttcg
     1621 caattaattc aatattattt ttttgccctt tattacctga aacattaaat ttaattggac
     1681 atcccgttat caattttata atatcatcaa gatttttatt atctgattca ggaattttat
     1741 ctgatataac aacaccattt tcaagatagt tcattaaatt atttgattca ctaacattag
     1801 tgttttgatc tccatcaagc caaaaataat ggttatcgga atctaaatac gatgagttta
     1861 aaatattatt acaaattatt tgatttgctc caccagggat atatctcact actaaattct
     1921 gtttaagatt ctcactacct gaatgagtga taacaaactc tagaatatat ttagctagtc
     1981 tatcttcaac ataaatcatc ttcctagaat gatacacatc acctaattca aaaaatgcat
     2041 cctgataatc aatattttca ataacatcta ccttttctcc gtttttcact aaaagtttca
     2101 cggcttctct aggaaaatct tttataagtt gtgtagaatg tgtagtgata ataatttgat
     2161 gttttttatt taaacactct tgaagtaaaa actctttaaa tttatagatt gcactcggat
     2221 gaagtgagat ttcaggttca tctattaata ttaatgaatt tgattgcgca tttactatat
     2281 catttactaa caaaataatt ctagcctcac ctgttcctgc aaaagcctca gaatattctt
     2341 ttccagattt tttcatccaa atagttttgg aagcttttat atcatcacct tttgaataca
     2401 acttatgtgt taaaatttga atgtctgtat aagattcatc cattatttca ctaataattt
     2461 cacaaacttt atcatcaact ttaacattat ctataaccat ttccttttta taacgcgtat
     2521 agctacttgt attattcttt aaaatatcag caactggttt agatcgtaat cttataaaat
     2581 cttgtttact acgttgagta gaaatttttt taaaattata gtgatagaaa aataaatcaa
     2641 aagcagaaac atattcttta caatcacaaa agacaacatt tttttcaatg ccatcccatc
     2701 tgtctgtcga agaacttcca atatatttat ttttgggtaa tctttccatc tcatattgtt
     2761 tttgaggagc atatggttcc caataatcta atcctttttt tgttccagaa cggcctttaa
     2821 gaacttctac atttctagaa gctttaacgt tataatatga atagattaaa cattgtttcc
     2881 catccacttc atctatttga tcaacatttg tactaaacca atattcagac acacttttat
     2941 tggctggaga accatataaa gcttgtaaaa ttgaagtttt atttactcca tatctattac
     3001 agacacctca ggattattta acttataagt tttaacagct acggaatcaa tttcaacagc
     3061 aacttgaaca tctatgcctg attttttaag gccacttgta gtgccacctg caccgctaaa
     3121 taaatcaata gcaacaattt tccccatagt attctcctaa agtttctcct ttttattata
     3181 acattatcaa atgtaaaacc caacccgaga gggttaggtt tttaacatca tttcaccaac
     3241 ttcttcatct catcaatacg tgcgacggtc gcgtcatatt tagcttggta gtcagcttgt
     3301 ttgtcgcatt ctttttggac gacttctggt ttggcgttgg ctacgaagcg ttcgttagag
     3361 agtttcttac caaccatgtc cagttctttt tgccatttag caagttcctt gtcgagacgg
     3421 gccagttctt cttcaacatt gaggagatcg gccagtggca ggtagatttc tgctcctgtg
     3481 atgacacttg acatagccag ttcaggtgca gggatggttg atgcgatttc caaatgttct
     3541 ggatttgtaa agcgtttgat gtagttgaca ttgctgttaa agaaggcttc caagtcgcta
     3601 tcgcttgtct taacaaggat ggtgataggt ttgcttggtg ctacatttac ttccgcacgc
     3661 gcattacgaa cggcacggat caagtctttg agactttcca caccagtgtg ggcagcaagg
     3721 tcctcaaagg ctgggttgac agttgggtat gctgctgtca cgatagagcc ttcagcgtac
     3781 tgaccaaaga tttcttctgt cacgaatggc atgattgggt gaagaagacg gaggatttta
     3841 tccagcgtat agaggagaac agatcgagta atgaccttat cgtcttcatt gtcgctgtaa
     3901 agaacttcct tggtcaactc aacataccag ttggcaaatt cttcccaaat gaagttgtag
     3961 aggatgtggc cagcaacacc aaactcaaac ttatcaaagt tagcagttgc ttttccaatc
     4021 gtttcattga ggttgtggag aatccagcgg tccgtcacat taccagcctc acctgttgca
     4081 acttttgtga cattgtcatg caccacatcc agcgtcaaac cttcattgtt cataaggatg
     4141 tagcgagaga tgttccagat cttgttaatg aagttccatg aagcatccat tttctcataa
     4201 gagaaacgca cgtcttgtcc tggcgcagaa ccgtttgaaa ggaaccatcg aagggcatcg
     4261 gcaccgtatt tctcgataac atccattggg tcaatcccgt taccgagaga cttagacatc
     4321 ttgcgtcctt gctcgtcacg aataagaccg tggataagga cgttttggaa tggttgacgg
     4381 cctgtgaatt ccaatgactg gaagatcata cgagacaccc agaagaagat gatgtcgtaa
     4441 cctgttacca aggttgaagt tgggaaataa cgtttaaagt cttctgagtc gacttcaggc
     4501 cagcccatgg ttgaaaatgg ccagagggca gaactgaacc aagtatccaa gacgtcttcg
     4561 tcctgagtcc atccgtcacc ttctggagct tcttcgccga catacatttc accatcagca
     4621 ttgtaccagg cagggatttg gtgaccccac caaagctgac gagagataac ccagtcgtgg
     4681 acattttcca tccattgaag gaaggtatcg ttgaaacgag gtgggtagaa ttcgaccttg
     4741 tcctctgtgt cttggttggc aatggcgttc ttagccaatt ggtccatctt gacgaaccat
     4801 tgagtagaca agcgtggctc aactacgaca cctgtccgtt ctgagtgacc gactgaatgg
     4861 acacgttttt cgattttgac aagggcaccg atttcttcca acttagcaac gactgcctta
     4921 cgagcttcaa aacgatccat gcctgaaaat tcaaaggcaa gctcattcat agttccgtcg
     4981 tcgttcatga cgttgacttg tggcaagtta tgacgttggc caaccaagaa gtcatttgga
     5041 tcgtgggcag gtgtgatttt cacgacacca gtaccaaact caggatctgc gtgctcatct
     5101 ccaacgattg ggatgagttt attagcgatt ggaaggatga cgtttttacc aatcaagtcc
     5161 ttgtagcgcg ggtcttctgg attaaccgca accgcaacgt ccccaaacat agtctcagga
     5221 cgagttgtag caacttcaag ggcgcgtgaa ccatcttcca gcatgtaatt catgtggtag
     5281 aaggcacctt ccacatcctt gtgaatcacc tcaatatcag aaagggctgt gcgagctgct
     5341 gggtcccagt tgatgataaa ctcaccacgg tagatccagc ctttcttgta aaggtccaca
     5401 aagaccttac gaacagcttt tgacaaacct tcgtcaagag tgaaacgctc acgagaatag
     5461 tctacagaga gccccatctt gccccattgt tccttgatgg tagtggcata ttcgtctttc
     5521 cattcccaga ctttcgtcaa gaaagactca cgaccaaggt catagcggga aatgccctca
     5581 ccacgcaagc gctcctcaac cttagcctga gtcgcaatac cagcgtggtc cattcctgga
     5641 agccaaaggg tatcaaaacc ttgcatgcgt ttttgacgga tgataatatc ttgcaaagtt
     5701 gtatcccaag cgtgaccaag gtgaagttta cctgtaacgt ttggtggtgg aataacgatt
     5761 gaataaggct tagccttttg atcgcctgaa ggcttgaaaa catcagcatc aagccatttt
     5821 tgataacgac cagcctcaac ctcggctgga ttgtatttag gtgaaagttc tttagacatg
     5881 tgtgtgtcct ttctctattt tgtttatttt attttgaatt tgcttagcag cttcttctgc
     5941 agacaaattc gtattattta ttttaaagta gtggttcaac tcattcggtt gatgttggga
     6001 atttaattga agtgtttcag cggtctctat aatttctctt tcagatacct caatatgtcg
     6061 ttttaagggt ttgtacttta atcgattctc cgttcgattt cgacgtatgc actcttcaag
     6121 acttgtttcc aactcaacaa aaagaatctc ttgatgataa tcatccaaca aactctgaat
     6181 ttgctttaaa tatatcagat gatactgatt tgaaaaatca attacatctg ttaaaattac
     6241 tgatcgctga tttcttgcac tcgctccaag gaatgaaaaa gtgattccac gaacaaattc
     6301 ccacatttcc tctgtatagt cctgatagat ttctagtgca aaatcaatgg cttgatggtt
     6361 ataaaagagg gtagcatccg tcagtcgaga taattcttga ccaatggtca tttttcctga
     6421 tgctggagca ccaatgatga aaagatgcat caaatcacct cccactcact cttcagaaag
     6481 gcatatctca aatcatcaca gcagttgcct tgagcatctt tgcggtctct tatgcgagct
     6541 tcgagggtaa agccaagttt ttccgcgact cgttgacttt gaaggttata tccaaagcaa
     6601 gttagttcaa tcttgtgaag acccaaatct ttaaaggcta agtcaatcaa ggcacgcgca
     6661 gcttctggca cataacctcg cccccaatag tctgggtgta aggtatagcc gatttccagc
     6721 acatcatctt catggcgatg gttgaaatcg acagagccaa cgatcttatc cgttcctttg
     6781 acgacaatcc cgtagcctgc tgggagattt tccttttgat tacgctccgg aagaatgtgc
     6841 tccagataat aaatctcatc ttccaaggtc ttgactggag gaaaacctgc tggataggcg
     6901 acctctggca aactagcata gtcaaagata tcctcagcat ccgccacagt acggactcgt
     6961 aaaacgagac attctgtttc gattttatct ggtagctcag ttcttgccat ccttcttcct
     7021 cgcttttttg atgaaactgc ccttcatatc tacacgcttg tccagatagc gataaacgcg
     7081 ctgatatcca tctcccatga aataggttgg ggcaaacagt tgatttttaa aatgtccctt
     7141 ttcatccagg agttctggaa caacaagtcg ctcaagaatc ttggcaaaga tgtggcaaat
     7201 accgtcttcc tcaacaatcc tatctacccg acaatctaaa acaagtggac aggcgtctaa
     7261 aataggagtc tgagttcgtt cagaaatttc ataatgcact cccaaacgtt ccaatttctc
     7321 ctgatgactg ataaaaccag cctgctccat cgcaagcata gaagtttcat cagaaatatt
     7381 cacagtaaat ttttgatact gtttgatctg ctctgcggca ttctctctcg caacgactcc
     7441 aatcacaacc caatctccta gactataaga ggaactacag gtcgtgatgt tatagccaaa
     7501 attctaatct tgatatccta aaataaaaac aggaaaacca taatatagtt tacttgtgtt
     7561 aaaagattgc ttcataacaa ccccctttga ctaagacgta aaagaaaagc cctgccatct
     7621 acatgacagg gacgaatgtg tttatccgcg gtaccaccca atttcgggca gttgcccgca
     7681 actctcgtct ttaaagcaaa aagacacttt tatttcaatt tttcatccat tagcaaccag
     7741 ttttgacaca tttgtggact tctcagcgcc gccactttct gtaaaatgtg ggattcaaaa
     7801 cacctctaat ggctctattg tagcaagatt ttttcggaaa tgcaaggctc aagaaaaatt
     7861 acttgaactt gatgccattt tctttcaatt tcttgatggt agctgggccg attcctttca
     7921 aggcaaggag ttctttttca gtccagtttt tgaaatctga agcagacttg attccttcat
     7981 catagaaagt cttggcacga tcaagtggaa gtccacccaa atttgctgca aaatcttcta
     8041 cagaattagc tactttttga gcttgctctt tggtagcttc tactgcttgt tcaacttttt
     8101 tagaaacagc cttaccagct gcgcttgctg actgttctgc acgcttcacg actttttttg
     8161 tctcatctac aaccttagtc acagtacttg aaaaggcacc tgcgcgacgg aggccatttc
     8221 gtaattgttt tttacgattg agtttctttg acatgataaa atcctttcaa taaaaaatcc
     8281 ctgctctgac aaggatttaa cataaacatt ctatcaagat tttagtgaaa aatcaagaat
     8341 ctttctcgtt taataatttc gctcaccaaa caagccttct ggcaaatcat ggaatccctt
     8401 gcctgctttg aagttttcaa acttaccaag caagaactga taagcatcca agcgactctc
     8461 gtagcgaatc atggcatctt tggcacgctc gtcctggtct tcttcgtaaa ctttttgact
     8521 ttccttgtgc gccatatcgt taatcatgat ttgggtattg acccaagctt caaattcctt
     8581 cataaattct tgttcgtaac tcattttttc tccttattct aatccacgaa aatagtccgt
     8641 atcaatctca cggtagggct ggatatcctg aacatactcc aacactcctt ggaattctcc
     8701 gtcttcatcg tgtactgcag cataggtgat gtggacaaac ttacctcgcg actcagactt
     8761 gaaccacatt tcatacttgt ctttgcttcc ctcacgaagc cccttcatga tagttttgac
     8821 cttgtccaag tacttaggcg gatggcagag ttcgacattg cgcccgactt gggacggcgt
     8881 ccgtttgaaa atcatctcat cagctggcgt attgtcattg taatactgga aaatatcttc
     8941 tttattgaca aaggtaatct ccatagggag atgattgagg atgagattgg cctgctcgac
     9001 tgaaagatag ccattaccaa aagcctgttg actatggcgg tccagcacag cttccttttc
     9061 cttaggggta aaggtaatgg taaaatggcc ttctggcgta tctatgactt gttgaacttg
     9121 accttctgcc gtatctagct gtacaggttc ctctgcaatc ttttcctcaa taaagctctg
     9181 tcgttctggc acccatttct ctgacggacg gatgatggca tagccatagg catcgctctc
     9241 ctccgcaatc tgaagccagt catcctgagt aaaagactca aggagaatca tgaggaggat
     9301 ggactcttcc ttgaaaatca tactttcaaa ctctgtcgca aaagcttcaa aagcttcctt
     9361 tacactgcta attgacactt ctggtagtga cttggctgtc gttagagctg tttgaaagag
     9421 ttccctaatc tgatcatcca ctccccacat aactttggga ggtgaatcgt gtccatagcg
     9481 ctccatgata ggaaagaaga gttcttcctt acgttggtaa tggatgtcaa attgccccac
     9541 aagccccatc tgacgcacca agcccttgcg catctccgca agcatttcct cgtcttccat
     9601 caactcatag gtatctaaca atctacgaat gcgaatcaaa gctgcacgga gagccagatt
     9661 ttcttctttg aagacacgaa ctgggtgacc tggatgctca gtatctgaaa cttcgacacc
     9721 tttgatagca tttttaaaaa gattggcatg gacatcacag agttccataa catcttcaaa
     9781 agtgacgccc gaatccgagt tcatcagctc gtgctccata agggaaatct cgatggctga
     9841 cacgcccgta aaggtcgcat caaagcgatc ttgaaccgac tcaggagagg cgccattgtg
     9901 caattctaac aaaatatccc gtaggacatg aatccgttca tctgccatta gtctaatcca
     9961 atcacttcgt agccattcgc ttccagtgtg cgtacaatct tgtccatagg agttcctgct
    10021 agcttagaac cctgtttaag tgatacttta cgaccaactg tattgcgcat taagggattg
    10081 gcaaggggtt taaaacccaa ctccactaga atttccaaga cttctggatg cttgtccacc
    10141 acttctgcaa caggaattga cacatcgatg atattgtcca tgacgacctc cattgtatgt
    10201 tctaatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggt tgctcaaaac
    10261 agtgctttga ggttgtagat agaactgacg aagtcagctc aaaacaccgt tttgaggttg
    10321 tggatagaac tgacgaagtc agtaacatat atactgcaag gtgaagctga cgtggtttga
    10381 agagattttc gaagagtata aaatgatttt actgcttata ttataccata tggaaagaga
    10441 ataaaaaact agcagtggag ttcctgctag ttctctgcga cttggattac catctagata
    10501 agtttaaaga gccaatccaa tatcttaaac actatcttgt aaaacagcaa ttggactgca
    10561 aaagagatga tcatccaaaa gaatttcaca atcccaataa tcggtttgat aaaaataatg
    10621 gcaagaagtc cccagaaaaa tttcattttt tatcctctgg cttaatgagc caccgagccg
    10681 tatccattag cttatctgca ataaagagtt ttcccagacc aacaacgaca tggtcatctt
    10741 tctttatcgt tttcattact tttacacctt gcacggtcaa aaagtaatta ccataaattt
    10801 tagctagagt ttttatgagt cccatattac tctccttcga tgatttcagc ctcttttcgg
    10861 attgtttcaa catcttcttg atattgaact tcactatagt taactagctt ggacaattct
    10921 ttctgcaagc tttcacccat cggtttcata gttgcaaagg ttaaaccacc tgaaatgata
    10981 ccacccaaga taggaataaa tttccccatt cctttggcca gccctccctt ggtaagattc
    11041 acaccaaata ttcttaagac ttttttcaaa ataggatacc aaagcgtctt tgttaaagct
    11101 ttattaggta ctgttttcat tacctgtttg gcaattgtta taccaccagc acgtagcaaa
    11161 gcagcagttc cattcacccc caacatgacg cctagataaa gcaagagggt attttgagca
    11221 tcttcactta actcctcacg tgaagtccaa agagcctcat aaccataaat ataacctaat
    11281 tcttgagcca atttcagaga gaaagcataa aattgagcca catcagctgg aatggtaatt
    11341 gccatagcaa gccctccagg taatcctgcc aaaacagaag tcccactcgc taataataca
    11401 ttgtcccgaa tacaagcatt agctacacga tctaatattt cttgagatag aagagtcgtt
    11461 ggaccttgtt ccaataaggt aggaatatct tttggatcca attctttgga aaacttatcc
    11521 actaaaaatt tcgaacgatc aaccttaaca acaggtagtt tcaccacttg ttgcaatact
    11581 tgcatagcta agtcttgttc agccatatac aaactcctta tttctttttt gcaccttatc
    11641 atattatatt tcctacaaaa aaagcatttt ctaattagat aacattaaaa aatacagaac
    11701 ccctttttta ta
//
LOCUS       DAVAAL010000049        11694 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVAAL010000049 DAVAAL010000000
VERSION     DAVAAL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11694
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.050"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(1570..3828)
                     /gene="glgP"
                     /locus_tag="VQM60_001142"
     CDS             complement(1570..3828)
                     /gene="glgP"
                     /locus_tag="VQM60_001142"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HET1081015.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(3854..5371)
                     /gene="malQ"
                     /locus_tag="VQM60_001143"
     CDS             complement(3854..5371)
                     /gene="malQ"
                     /locus_tag="VQM60_001143"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET1081016.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            5921..7192
                     /locus_tag="VQM60_001144"
     CDS             5921..7192
                     /locus_tag="VQM60_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET1081017.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGTKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            7303..8595
                     /locus_tag="VQM60_001145"
     CDS             7303..8595
                     /locus_tag="VQM60_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET1081018.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            8597..9439
                     /locus_tag="VQM60_001146"
     CDS             8597..9439
                     /locus_tag="VQM60_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET1081019.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            9692..10492
                     /locus_tag="VQM60_001147"
     CDS             9692..10492
                     /locus_tag="VQM60_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET1081020.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     SSQIKELPKNLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            10502..11488
                     /locus_tag="VQM60_001148"
     CDS             10502..11488
                     /locus_tag="VQM60_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET1081021.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
BASE COUNT         3527 a         2460 c         2155 g         3552 t
ORIGIN      
        1 atctgcaacc tcaaagctgt actttgagca acctacggct agtttactct ttaattttca
       61 ttgagtataa actagacttc gcaaaagtag aaaacttacc gaaaatcttc aatatgacaa
      121 aaacgtataa gaacaggtac acaaacctta atcttatgcg ttttgttata gtttccatac
      181 acattaaggg gaaatagttg aaatcagttc ttttaataac cattgaatac catatactac
      241 atttacccca aatatgatat aggccgtata aatcaagtat ttcgattttt cccgcctcaa
      301 agaatagatc ttatacaaaa taaataaatt taaaataata aataatagag acaaaatctt
      361 cctccaaaat caatcctaaa agtagccagt caagcatagc tctaatcttt attttcgatt
      421 cgatataaca aacttatcaa tgaatagtaa gtgatataag gccagtacca gaccccaaac
      481 gctaccacga aaagagaatt cttgatgatt gagagtaaac aaatcatggt taccaatata
      541 gaggcaggtc aaaataacaa ataaaatcca aaataaatcc gtccatttat attttgaaaa
      601 atattttttt aacataatag catatttttt tgactataat aacagtatat gataaaatgt
      661 aagcagatac aaataaaaaa gagagaaaag gaatgatctt ataccagatc attccttttc
      721 tgctttctgt aaatccctcg gagtgtgttt ggcgctttga tacgaagtgt caaagctgta
      781 taaacgttac tatttccaaa aaagtaacag aaaattcctg ctttgtagga ctgctttggg
      841 tgagtaatag acatgtcgac tcaccctatc tcctgatggc gaaaagaatt tagcaatggt
      901 gcaagttgtt ctgaactcga gaaaaaacat gccgaaatcg aagaaaagcg tgcagaactt
      961 gaagcgaaga agcaagaaat gttgaagcgc ttggataaat agaatttaga aaacagaaaa
     1021 aactaaaaag aatgtagggt ttatttccct acattcttaa aataacttag tacataaatg
     1081 acaatgctgc cactgccgtc aatattgcaa aaactatttt ccaaattttt ttattttttc
     1141 caaaaatctt atcaggtaac aagcttatta caagaaaggt aataaacaac accaatttaa
     1201 gtgtgtatat cataaaaaca ccttctttcc tacaattata ttataatact ttatgtaagc
     1261 gaatacaaga ggtgtgaggt aatcaactac gaaaaaatat aaggctgagg acagtaaaaa
     1321 ccaacattta aagccttcac tgtatctagg atttccctat ttttagacaa gattgaccca
     1381 aatctacatc attaaaaaag cgaatttcaa tcgaaactcg cttttttaca agatgtatta
     1441 gtataaattt gtatcttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca
     1501 ggttgctcaa agcaccgctt tgaggttgca gataaagctg acgtagtttg aagagatttt
     1561 tgaagagtat tagttcaagt gccagatgtc ttcgttatac tgagcgattg tacggtcaga
     1621 tgagaagaat cctgctttag aaatgttaac gatgacttta tccaaccatg cgtcacggtc
     1681 ttcgtagtca gcaagcattt gctctttgac tttgatgtag tcttccaagt caagaagagt
     1741 catgaaccag tctttgttga tcaattcatt gtaaagacgt tccaagcgct ctttgtttcc
     1801 agctgcaaga actgcatcac taacgatgaa gtcaaccaat ggtttgatag cttcacgagc
     1861 gtagaattcg cttgatttgt aagctgcttt tgcgtaaagg tcgataacag tttctgaatc
     1921 ttcaccgaag atgtagatgt tttcttctcc aaccaactca gcgatttcca cgttagcacc
     1981 gtccatagta ccaagtgtca aagctccgtt caacatgaat ttcatgttac cagtacctga
     2041 agcttcttta gaagcaagtg agatttgttc tgagatatca catgctggga taaggaagct
     2101 tgctgcagta acgttgtagt tttcaaccat aactacttgc aagtgtggag ctactgctgg
     2161 atcgttagca ataacttctg acatgcaaag gatcaagtga atgatatctt gagcgattgt
     2221 gtaggctgga gctgctttac caccaaagaa gattgtgatt ggacgagcag ggatgttacc
     2281 agctttgatg tcaaggtatt tgtggatcac gtacaaagcg ttcatttgtt ggcgtttgta
     2341 ctcgtgaaga cgtttgattt ggatatcaaa gatagaattt ggattgattt ccacaccttg
     2401 gtgttctttc aagtgacgag ccaatttacg tttgttgtga gccttgatgc tttccaattt
     2461 ttctttgaca gctgctttgt cttcataaga caacagtttt tcaagctcat ctgcttcatg
     2521 gtgccaacca tctccaagaa tctcatccaa gtagtgagac aatcttgggt tagcatgcat
     2581 aagccaacga cggaaagtga taccgtttgt tttgttgttg aacttttctg ggtaaaggtc
     2641 gtagaaggct ttcaactcag aatttttcaa gatttcagta tggagtgctg caaccccgtt
     2701 aacactgtat ccgtagtgga tatccatgtg agccatgtga acacgtccgc tctcatcgat
     2761 gatttgaaca gctggatctt tgtactctgc cttcacacga cggtccaatt cttcgatgat
     2821 tggtaccaag tgaggaacca cttcttgcaa gaattcaaga ggccattttt caagcgcctc
     2881 agcaaggatt gtgtggttag tgtaggcagt catgctacga acaattgaga ttgcttcgtc
     2941 aagctcgata ccacgtgcag tcaaaagacg aatcaattca ggaatcacca ttgatgggtg
     3001 agtatcgttg atttggacaa ctgcgtagtc agcaaggtca tgcaagttgc ttcctttttc
     3061 gattgcttcg tcgatgatca attgcgcacc gtttgaaacc atgaagtatt gttggaagat
     3121 acggagcaat tcaccttgac ggtcactatc atctgggtaa aggaagagag ttaagttgcg
     3181 agcgatatct gtcttgtcaa agttgatacc atctttaata atagaagaat caactgaatc
     3241 caagtcaaac aaacgcaagc ggtttttagt cgctgtttca taaccagtaa catcaatatc
     3301 gtaaagagtt gatgtcaaag taaagtctgc aaatggtact tggtagctac ggcttgagcg
     3361 aaccaaccag ttttgctctg tcaaccatgc atttggaatt gtttcttgtt ggttgttttt
     3421 aagaacttgt tggaaaagac caaagtggta gttaagacca acaccgtcac cattcaaacc
     3481 aagagtagca attgagtcga taaagcaggc agccaaacgt cccaaaccac cattaccaag
     3541 agatggttcc aattcaactt cttcaacttc gatcaagtct ttacctgcag ctgcaagttc
     3601 ttttttaaca tcgtcgtaaa gaccaaggtt aatcaagttg tttgacaaga gtttaccaat
     3661 caagaactca gctgagatgt agtaaacttt tttcttacca gtgttgactg gtttttggct
     3721 gcttgcaagc ttgctgtagt taagaagagc aaggtaaagc tcttcattgc tacattctgc
     3781 aatggtttta ttgtaacgat tttgtacaaa ttcttgtagt gataacatgt ttagttgtct
     3841 cctgattatt gtcttatttc tttaaatcta ccaaattttc attaattcgg cgataaattg
     3901 ttgtcaagtc aagcaaacct tcctcgacag ctggtgtcaa ttgatcttca gtcatacgcc
     3961 aagaccagtt tccaccaagg gtagatggga agttcatacg agctgcctca tccaattcta
     4021 gtaaatcttg catagttgca attgccataa agctaactga tgaaaatact gtacgaagca
     4081 tagcgtgtac cactgtttcg tattctttac ggttcgtgta acgagccatg tactcacgag
     4141 tcgcatcatc aatctcatta cggtaccaac caagaaccgt attgttatcg tgtgttcctg
     4201 tgtacataac tgagttagca ggtgccaagt gtgggctatc aatactttcg tcttctgggt
     4261 tgaaggcaaa ttgaagaatc ttcattcctg ggaagccagt acgttcacgc aattcgatca
     4321 cttcatctgt catgaagcca aggtcttctg cgatgatgtt tagctcacca agttcttcct
     4381 taacggctgc aaaaagcttg tagcctggac ctttcaccca ctcaccaggt gctgctgtat
     4441 cggaaccagc agggatttcc caataagatt cgaagccacg gaagtggtcg atacgaacga
     4501 tatcgtagat tttgaagctt tcacgcaagc gttcaatcca ccatttgtag ccgtctttgt
     4561 ccattgcttc ccagtcatag attgggttac cccaaagctg accagttaca gaaaactcat
     4621 ctggtgggca tcctgcgata caagtagcct taccattgac atctgttttg aagagatgtg
     4681 gatttgccca catatcactt gaatcttccg ctacgtagat tggcatgtcc ccaacgattt
     4741 cgatgtggtt gtcgttagcg taagctttca atttcaacca ttgttggaag aagaagtatt
     4801 gagtcacacg gtggtaaacc aacttgtctg ccaattgctc acgatagctt tcaagtgctg
     4861 aagctttacg agcacgagca tctgcatctg gccattcagt ccaagcaaga ttgtcaaaat
     4921 actctttgat agccatatac tcagcaaaga gctcaagcca tgattggttg tcttgagcaa
     4981 atttctcaaa atctttaaca tctccgactt caaagaaacg tttcaccgct ttttctaaaa
     5041 gaggacgacg tgcatagtag attttagcat agtcaacttc agacgcatcg ctaccaaagt
     5101 caactccttc aaggtcactt gcttccaaca aaccttgctc caccaagata tctaaatcga
     5161 taaaatgagt gtttcctgcg aaggctgaga aagattggta aggagaatcc ccgtaactag
     5221 ttgctcctaa tggaaggatt tgccagtaac gttgttttgt acggaccaag aaatcaacga
     5281 agtcgtaagc actttgacca aatgatccga ttccgtaagc tcctggaaga gaagagatgt
     5341 gcatcaacac accactttga cgttttttca taatagcacc tcgtgtgtta aaataatgga
     5401 acgttgcgta tttttcagac gcaaacgttt gcgttactta taagtatact ccttttcaag
     5461 cataattgca agcgttttta aaaaactttt ttgattattt ttaaggagac taactcattt
     5521 tagcaaagat aacttaaaac tatctaatcc taaggtttga ctctcacgag acagtctaca
     5581 aagtacaaaa cctccttagg gagcttgaat ttatatgcaa caaagcacaa acgtctatag
     5641 aaaatagata gggattagaa ccagggaggt agcccctcct gatttccctc tttagacaga
     5701 ttccatatat ttttattaaa aatactgtat tcttatctat ttcatacttt tcttctatta
     5761 aaatgcaaaa ttatgctatc aaatcaagct taaaaatttt ttaaaatttt tacaaaaaat
     5821 acttgcaacc gttttctatt tgtgctatac taagctcata aaggaaaacg tttgcgtttc
     5881 cttatcaata aaacttgcta ttctttggga ggaatacact atgtcatcta aatttatgaa
     5941 gagcactgcg gtgcttggaa ctgttacact tgctagcttg cttttggtag cttgcggaag
     6001 caaaactgct gataagcctg ctgattctgg ttcatctgaa gtcaaagaac tcactgtata
     6061 tgtagacgag ggatataaga gctatattga agaggttgct aaagcttatg aaaaagaagc
     6121 tggagtaaaa gtcactctta aaactggtga tgctctagga ggtcttgata aactttctct
     6181 tgacaaccaa tctggtaatg tccctgatgt tatgatggct ccatacgacc gtgtaggtag
     6241 ccttggttct gacggacaac tttcagaagt gaaattgagc gatggtacta aaacagacga
     6301 cacaactaaa tctcttgtaa cagctgctaa tggtaaagtt tacggtgctc ctgccgttat
     6361 cgagtcactt gttatgtact acaacaaaga cttggtgaaa gatgctccaa aaacatttgc
     6421 tgacttggaa aaccttgcta aagatagcaa atacgcattc gctggtgaag atggtaaaac
     6481 tactgccttc ctagctgact ggacaaactt ctactataca tatggacttc ttgccggtaa
     6541 cggtgcttac gtctttggcc aaaacggtaa agacgctaaa gacatcggtc ttgcaaacga
     6601 cggttctatc gtaggtatca attacgctaa atcttggtac gaaaaatggc ctaaaggtat
     6661 gcaagataca gaaggtgctg gaaacttaat ccaaactcaa ttccaagaag gtaaaacagc
     6721 tgctatcatc gacggacctt ggaaagctca agcctttaaa gatgctaaag taaactacgg
     6781 agttgcaact atcccaactc ttccaaatgg aaaagaatat gctgcattcg gtggtggtaa
     6841 agcttgggtc attcctcaag ccgttaagaa ccttgaagct tctcaaaaat ttgtagactt
     6901 ccttgttgca actgaacaac aaaaagtatt atatgataag actaacgaaa tcccagctaa
     6961 tactgaggct cgttcatacg ctgaaggtaa aaacgatgag ttgacaacag ctgttatcaa
     7021 acagttcaag aacactcaac cactgccaaa catctctcaa atgtctgcag tttgggatcc
     7081 agcgaaaaat atgctctttg atgctgtaag tggtcaaaaa gatgctaaaa cagctgctaa
     7141 cgatgctgta acattgatca aagaaacaat caaacaaaaa tttggtgaat aaaaaatttg
     7201 ttcaaggggg gtggaaatca aatccccctt tgaatttatc aatagagaca caaataattt
     7261 agctttctta taaaaaagta gtatcctatg aaaggagtta atatggaaaa gcaacaacct
     7321 agtaaagcag ccctgctgtc tatcattcct gggttaggac agatttacaa taaacaaaaa
     7381 gccaaaggtt ttatcttcct tggtgtaacc atcgtatttg tcctttactt cctagcactt
     7441 gcaacccctg aattgagcaa cctcatcact cttggtgaca aaccaggtcg tgataattcc
     7501 ctctttatgc tgattcgtgg tgccttccat ctaatctttg taatcgttta tgtactcttt
     7561 tatttctcaa atatcaaaga tgcacatacg attgcaaaac gcattaacaa tggaattcca
     7621 gttccacgca cactcaaaga catgatcaaa gggatttatg aaaatggctt cccttacctc
     7681 ttgatcattc catcttatgt tgccatgacc ttcgcgatta tcttcccagt tatcgtaacc
     7741 ttgatgatcg cctttaccaa ctacgacttc caacacttgc caccaaacaa gttgttggac
     7801 tgggttggtt tgaccaactt tacaaacatt tggagcttga gtaccttccg ttctgccttt
     7861 ggttctgttc tttcttggac tatcatttgg gctttggcag cttctacttt acaaatcgta
     7921 attggtatct tcacagctat cattgccaac caaccattta tcaaaggaaa acgtatcttt
     7981 ggtgttattt tccttcttcc ttgggctgtc ccagccttca tcactatctt gacattctca
     8041 aacatgttta acgatagtgt cggtgctatc aacactcaag tattgccaat cttggctaaa
     8101 ttccttcctt tccttgatgg agctcttatt ccttggaaaa cagacccaac ttggactaag
     8161 attgccttga ttatgatgca aggttggctc ggattcccat acatctacgt tttgactttg
     8221 ggtatcttgc aatctattcc taacgacctt tacgaagcag cttatattga cggtgccaac
     8281 gcttggcaaa aattccgcaa catcactttc ccaatgattt tggctgttgc ggcacctact
     8341 ttgattagcc aatacacctt caactttaac aacttctcta tcatgtacct cttcaatggt
     8401 ggaggacctg gtagtgtcgg aggtggagct ggttcaaccg atatcttgat ctcatggatc
     8461 taccgtttga caacaggtac atctcctcaa tactcaatgg cggcagctgt taccttgatt
     8521 atctctatca ttgtcatctc aatctctatg atcgcattca agaaactaca cgcatttgat
     8581 atggaggacg tctaagatga ataactcaat taaactcaaa cgtagactga ctcaaagcct
     8641 tacttacctt tacctgattg gtctatcaat tgtaattatc tatccactgt tgattaccat
     8701 tatgtcagcc tttaaagcag gtaacgtctc agcctttaaa ctagatacta atatcgacct
     8761 caattttgat aactttaaag gcctcttcac tgaaaccttg tacggtactt ggtacctcaa
     8821 cactttgatt atcgccttaa ttaccatggc tgttcaaaca agtatcatcg tacttgctgg
     8881 ttatgcttac agccgttaca acttcttggc tcgtaaacaa agtttggtct tcttcttgat
     8941 catccaaatg gtgccaacta tggccgcttt gacagccttc ttcgttatgg cgcttatgtt
     9001 gaacgccctt aaccacaact ggttcctcat cttcctctac gttggtggtg gtatccctat
     9061 gaatgcttgg ctcatgaaag gctacttcga tacagtgcca atgtctttag acgaatctgc
     9121 aaaactagac ggtgcaggac acttccgccg cttctggcaa attgttcttc cacttgttcg
     9181 cccaatggtt gccgtacaag ctctctgggc cttcatggga cctttcgggg actacatcct
     9241 ctctagtttc ttgcttcgtg agaaagaata ctttactgtt gccgtaggtc tccaaacctt
     9301 cgttaacaat gcgaaaaact tgaagattgc ctacttctca gcaggtgcta tcctcatcgc
     9361 ccttccaatc tgtattctct tcttcttcct acaaaagaac tttgtttcag gacttacaag
     9421 tggtggcgac aagggataat ttatccccgc cacccttttt cattttatac tcttcgaaaa
     9481 tctcttcaaa ccacgtcagc tttatctcca acctcaaagt tgtgctttga gcaacctgtg
     9541 gctagtttgc actttgattt tcattgatta ttagcaattg tcactgtaaa taatatcctt
     9601 gtagcaagca atttttctcc tagacttgaa ataaagcgca tttctctata taataatact
     9661 catatagaaa acacctttta gaaagatacc tatgcttcca tatccatttt cctatttttc
     9721 aagtatttgg gggtttcgta agcccctgtc caaacgtttc gagctcaact ggtttcaact
     9781 tctctttacc agtatcttcc ttatcagctt gtctatggta cccattgcta tccaaaacag
     9841 ctcccaggag acctatccgc tagaaacttt tatcgataat gtctatgaac ctctgacaaa
     9901 tgaggttgtc caggatctct ctgaacatgc tacaattgtc gatggcacat taacttatac
     9961 tggaacagtt agtcaagcct cttctgttgt gattggttca agtcaaatca aggaattacc
    10021 taagaacttg caactgcatt tcgatacaaa tgagctagtc atcagcaagg aaagcaagga
    10081 actgacccgc atctcttacc gagccattca gactgagagt ttcaaaagca aagacagctt
    10141 gacccaagca atttctaaag actggtacca acaaaatcgt gtctatatca gcctcttcct
    10201 agttctcggt gcgagcttcc tctttggttt gaatttcttt atcgtctctc taggagctag
    10261 tcttctcctt tatatcacta agaaatcacg cctcttttca tttagaacct ttaaagagtg
    10321 ctatcacttt atcttgaact gtttaggatt gccgactctg attacactta ttttgggatt
    10381 atttggccaa aatatgacaa ccctgattac tgtacaaaat attctttttg ttctgtatct
    10441 ggtcactatc ttttataaaa cacatttccg tgatccaaat taccataaat aggagatttt
    10501 tatgcccgtt acgattaaag acgtggccaa ggctgctggt gtttcgcctt caaccgtaac
    10561 ccgtgttatt caaaataaat caaccattag cgacgaaaca aaaaaacgcg ttcgcaaagc
    10621 tatgaaggaa ctcaactacc acccaaacct caacgctcgt agcttggtaa gcagctatac
    10681 tcaggttatc ggattagttc ttcctgatga ctcagacgcc ttctaccaga atcctttctt
    10741 tccatcggtt ctacgtggca tctctcaagt cgcatctgaa aaccactatg ccattcagat
    10801 agcaacaggg aaagatgaga aggagcgtct caacgctatt tcacaaatgg tctacggcaa
    10861 gcgtgtagat gggctaatct ttctctatgc ccaagaagaa gaccctctcg taaaactcgt
    10921 cgcagaagaa cagttcccct tccttatctt aggtaaatct ctatctcctt tcatcccact
    10981 tgtcgacaac gacaatgttc aagctggttt tgatgcgact gaatatttca tcaaaaaagg
    11041 ctgcaaacgc attgccttta tcggaggaag taaaaagctc ttcgtgacca aagaccgttt
    11101 aacaggctat gaacaggcgc ttaaacatta caaacttacc actgacaaca atcgcatcta
    11161 ctttgccgac gagtttctgg aagaaaaggg ctataaattt agcaagcgat tattcaagca
    11221 cgatccacaa attgatgcta tcatcacaac cgatagcctc ctagctgaag gtgtttgtaa
    11281 ctatattgcc aaacaccagc tggatgtccc tgttctcagc tttgactcgg ttaatcccaa
    11341 actcaacttg gcagcctatg tcgatatcaa tagtttagag cttggtcgtg tttcccttga
    11401 aactattctc cagattatta atgataataa aaacaataaa caaatttgtt accgtcaatt
    11461 gatcgcccac aaaattatcg aaaaataaga gactgggcaa aaagtcgtta aaagcaaaaa
    11521 cgcatagtat caggtattga aaaaacttga tactatgcgt tttaatgtgg gaagatttac
    11581 ttccttttct actgaaattg agtcttttcc caagatcttt ttatactcaa tgaaaatcaa
    11641 agtgcaaact aggaagctag ccgcaggttg ctcaaaacac tgttttgagg ttgc
//
LOCUS       DAVAAL010000050        11544 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVAAL010000050 DAVAAL010000000
VERSION     DAVAAL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11544
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.051"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            431..1141
                     /gene="deoD"
                     /locus_tag="VQM60_001149"
     CDS             431..1141
                     /gene="deoD"
                     /locus_tag="VQM60_001149"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET1081022.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1164..1688)
                     /locus_tag="VQM60_001150"
     CDS             complement(1164..1688)
                     /locus_tag="VQM60_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET1081023.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1741..1977)
                     /locus_tag="VQM60_001151"
     CDS             complement(1741..1977)
                     /locus_tag="VQM60_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET1081024.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSTKLAK"
     gene            complement(2045..2965)
                     /locus_tag="VQM60_001152"
     CDS             complement(2045..2965)
                     /locus_tag="VQM60_001152"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET1081025.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3155..3558
                     /locus_tag="VQM60_001153"
                     /pseudo
     CDS             3155..3558
                     /locus_tag="VQM60_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3774..4355
                     /locus_tag="VQM60_001154"
     CDS             3774..4355
                     /locus_tag="VQM60_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET1081026.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4352..5629
                     /locus_tag="VQM60_001155"
     CDS             4352..5629
                     /locus_tag="VQM60_001155"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET1081027.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5647..6309
                     /gene="deoC"
                     /locus_tag="VQM60_001156"
     CDS             5647..6309
                     /gene="deoC"
                     /locus_tag="VQM60_001156"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET1081028.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIESCLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6296..6685
                     /locus_tag="VQM60_001157"
     CDS             6296..6685
                     /locus_tag="VQM60_001157"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET1081029.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPILPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6776..7828
                     /locus_tag="VQM60_001158"
     CDS             6776..7828
                     /locus_tag="VQM60_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET1081030.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7972..9507
                     /locus_tag="VQM60_001159"
     CDS             7972..9507
                     /locus_tag="VQM60_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081031.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            9500..10558
                     /locus_tag="VQM60_001160"
     CDS             9500..10558
                     /locus_tag="VQM60_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1081032.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10561..11517
                     /locus_tag="VQM60_001161"
     CDS             10561..11517
                     /locus_tag="VQM60_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1081033.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3292 a         2056 c         2635 g         3561 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaca gaaaggtaga gcgcgtgttc taatttgaac
       61 acgagtagaa aacttttcta aaaacaaaaa cgaaaggatg ggtaaactgt attcgctgaa
      121 ctgaatacgg gcgactctcc tctaaatcaa aattaagaaa ggaattgacc ccaccctaaa
      181 agtagtggga aaaagatagt tggtctagcg agcatcgctc actgcgccca actcctattt
      241 tcccttcgct ttttgatggg tttggtatct ttctcaatat aaaatataaa ataaagaaag
      301 gtagagcgtg tgttttgatt tgaacacgag cggaaaactc ggaaaataga taatctgact
      361 gaaaaatcag gatttctcgt caggttccta attttcagtc gttttcttct cgctctttgt
      421 atcataaatt atgtctatcc atattgctgc tcagcagggt gaaattgctg ataaaattct
      481 tcttcctggg gatcctcttc gtgctaagtt tattgcggag aatttccttg gtgatgctgt
      541 ttgttttaac gaagtgcgta acatgtttgg ttacactggt acttacaagg gtcaccgtgt
      601 atctgtcatg ggaactggga tgggaatgcc atctatttcg atttatgcgc gtgagttaat
      661 cgtagactac ggtgtgaaga aattgattcg tgtgggaact gcaggttctt tgaatgaaga
      721 ggttcatgtt cgtgaattag ttttggcgca ggcggctgca accaactcaa acatcgttcg
      781 taatgactgg ccacagtacg attttccaca aattgctagc tttgatttgc ttgataaagc
      841 ctaccatatc gccaaagaac ttggtatgac tactcacgtt gggaacgttt tgtcatctga
      901 tgtcttttac tcaaattact ttgaaaagaa tatcgagctt ggtaaatggg gagtcaaggc
      961 tgtggaaatg gaagcagcag ctctttacta tcttgctgcc caataccatg ttgatgcgct
     1021 agctatcatg accatctctg atagcttggt caatccagac gaagacacaa ctgcagaaga
     1081 acgtcaaaat accttcactg atatgatgaa ggttggtttg gaaaccttga ttgcagaata
     1141 attatagcca aaaaggaagc cctttatgaa ttgtaggact tccttttctt atccagaaat
     1201 tgatctagct ctctctgatt tcgaagaata gtgactttat gtgaatattc ttggcaaagt
     1261 ttttggtaat tttctttttg agttttgcta cgcccatccc aaagaatcca tctgataaac
     1321 tcccagtcaa agcgttcagg gcaatctgcc gccatacttt ctctgacttt tccacggtat
     1381 ttaagataac gcttaaaggc tctaaagaga caggtcaatg gcaaaaaatt gagaaagatg
     1441 atttggtcag cttcttgcat tcgttcttgg tagtagcacc aagaataatt accatcgatg
     1501 acccaagctt tatgcttggt gagaaagttt tttatctcgg ttaacatcca ttcgcagtca
     1561 ctgtcttgcc aaccaggttg aaattggagt gtgtccatgt gcagttttgg aatggagtag
     1621 tagttagata acttttctgc tagagttgac ttaccagaac cagaatatcc gataattgtg
     1681 attttcattt tctacctttt cctatttgga gacaaaaaaa cagcctctat ggactgtttc
     1741 ttatttagca agtttagttg aaagacgagc tttatcgcgg cttgctttgt ttttgtgaat
     1801 caaaccttta gtttctgctt tatcgatagc tgagctagca gcacggaaaa gttcttcaga
     1861 tgggtttgct tcgaaagctt ttatagcagt acgcatagct gatttttgag ctgagttctt
     1921 ttcgttttgt ttaacgttca attcagcgcg tttgatagct gatttaatgt ttgccaatgt
     1981 tcttacctcc atatttacta actataccat tatatatgaa aacttacgtt ttgacaaggg
     2041 aaaattactt ttttaagtaa atttcatcga tttcatggtt cttgctttta tgaagaatca
     2101 cttctgcacg atttctggtt ggttcaatat aattttgcag atttgtgaga ttgatactga
     2161 tccagacctg atgggcaaag gactccactt ccccaatcgg catctgagta aaacgataat
     2221 agtagctatc agggtcgttt tgggctaggc tcagcatttt caagaaacgg tccagatacc
     2281 aactttcaat atcatccact ccagcatcta catagatgga aaagtcaaag aagtcagtga
     2341 tatagagacg atcgttttgt ggattttgaa agacattaat tccctcaaca attacaaaat
     2401 cagcagcttt gacactttgt ttcttttcgg gtacgatgtc gtaaacttca tgagaataga
     2461 caggaatatc tacatcttgt ccatttttga tgcggtccaa gaagttgaga agagcttcca
     2521 tatcatagct ttcaggaaat cctttacgat ttaaaatccc ctgctcaatc aaggtttgat
     2581 tgggatatag aaaaccatca gttgtaacca actcaaccgt agcatctgta aacgtacggg
     2641 acagtaggat ttgaagtagg cgactggttg tggattttcc aacggcaaca ctcccagaaa
     2701 ccccaataat aaaaggttga gatttacttt cacgttggag gaaaattcct tttgaaaagg
     2761 ctaaatcttc cttagttcgc ttgtaaatct gaatcagatg agccaagggg agatagatat
     2821 ctgtaacgtc ttggagactg atttggtcat taaaactctt gatagattcc aattcttctt
     2881 ctgtcaaagg aggtgttgtc tttcgatgta aagattgcca agtctggcgg ctgatttttt
     2941 caaaatgtaa aaattcgttg gtcatttgtt ttcccctaga tattttgtct atcataccat
     3001 aaaaagctaa aaaaataaag cggtttcttg aatgtggtaa gtcaatggta tatagtataa
     3061 gtgatcaatt tattgatgaa ataggagata gattctactg atttataagc ttataaaggg
     3121 ggctttttag ttcacaactt ataggaggtg tactatgaaa atcttaaaac gttacatatt
     3181 ggaactctgt tttattttaa gttttgcttt accttttata aaaggaacca atgcagataa
     3241 tggtagatgc tttgtggaaa cctattacgg ttttactttt ttgatggaac atgctattgt
     3301 aacagctgtc tttatctgtt cgttcttaat tgctttctta ctaaaaaacg atggacgaaa
     3361 tggattgctg cgggtagtta ttgcttttta gtcctacgga ttgcgacaga gggttatttc
     3421 ttccacatgt cactagaaga tttgatacga ctttggacaa gtttggaaat cctgacacaa
     3481 acgtatcagt tgggctttta tctaaacatt ttgttaggaa ttttgttgat aataaagtat
     3541 tttaaggtta agcaatagaa ataataatct gcctgattat agatgataaa ctttttgttt
     3601 ttatctggac tgtgatattt atagttctaa atggtttatt atttataagt gttgaaagag
     3661 attgaagaaa atggcttcgt gacttgcttt cttcggtctt tttcgtttta ttatcaattt
     3721 gtaaacgatt tacaatttga gtcaaattat ggtaaaatag tgtcatgagt aaaatgtact
     3781 atgcagaaaa tcctgacgct gctcacgaca ttcatgagtt gagagtggac ttgttgggag
     3841 aaaaaatgac ttttttgaca gatgcgggtg tttttagcaa gaaaatggtt gactttggaa
     3901 gtcaactctt gctcaagtgt ctggatgtca accaaggaga gacagtccta gatgtaggct
     3961 gtggctatgg tccattaggt ttgtccttgg tcaaggttta cggagttcag gcgaccatgg
     4021 ttgatattaa cactcgtgcc ctggatttag ctcagagaaa tgctgaaaaa aataatgcaa
     4081 aagcgacgat atttcaatct aatatttatg caagagttca agggcgattt gaccatgtca
     4141 tttccaatcc tcctatccgt gcgggcaagc aagtcgttca tgaaatcatc gagaaaagta
     4201 aagatttctt ggaaattggt ggagatttga ccatcgtcat ccagaaaaaa caaggagctc
     4261 caagtgccaa gtctaagatg gaagatgtgt ttggcaattg tgaaatcctt aaaaaagaca
     4321 agggatacta tatccttaga agtgtgaaag aatgagagca gttgatttaa tccaaaaaaa
     4381 acgagacggt caagaactga cttcaagtga aattgaatgg ctgatagaag gctatgtatc
     4441 aggaactgtt cctgattatc agatgtctgc ctttgctatg gctgtttatt ttaaaggaat
     4501 gacgactcga gaaatctctg atttaacgat gaatatggtt aagactgggc aagagtttga
     4561 cctgtcagcc attgatggtg tgaaagttga caagcattct actggtggtg tcggcgataa
     4621 ggtaaccttg attttagctc ctcttgttgc tagcttcggt gtgcctgtag caaaaatgag
     4681 tggtcgtggt cttggccata caggcggaac aattgataaa ttggagtcca ttaagggcta
     4741 tcaagtagaa cgtagtcaag aggattttat tcgtcaggtt caggatattg gagtatctgt
     4801 cattgggcaa tcagaccagc tggtcaaagc agataagctt ctctatgccc ttcgtgatgt
     4861 gaccgcaact gtcgacacaa ttcctttgat tgcgagttcg gtgatgagca agaaaattgc
     4921 ggcaggggcg gatgctattt tgctagacgt gactgtcggt gagggtgcct tcatgaagac
     4981 ggttgatgag gcgcgtgagt tggctcaaac tatggtagat cttggtaagg ttgttggacg
     5041 gaagacagta gcagtgatta cggatatgag ccagcctttg ggacgagcga ttggaaatcg
     5101 tctggaaatc cttgaagcat tggagatttt gcaaggtcaa ggacgtcagg atattaccca
     5161 ctttatttgt gaattggctc aaattatgct tggtttggca aatgttaaca aaactgttga
     5221 agaagttcgc caacatcttg aaaatggtca agcactggct aagtttgagg aaatggtgca
     5281 agcccaaggt ggagacttgg aagacctcta tcgtcctgta aatgtagctc atgtggtgga
     5341 aatccctgct caggaaacgg gtgtcatttc agctcttcca gctatggact ttggtcttta
     5401 tgccatgagg ctgggtgctg gtcgtgcagt taagtctgat gtcttggact atgaaacagg
     5461 aattgttttt gaaaagaaga ttggagactc cgttcaaaag ggtgaaattg ttgcaaaagt
     5521 atacacaaat gaaaaaattc cacctcaact agttacagat ttccaaaaat gtgttaaaat
     5581 aagtgatgaa gtgaaaaaaa tccgtgaaat tgtagaaatc atctcctaaa tggagggaaa
     5641 accgaaatga aattaaataa atatatagat catacgcttt taaaacaaga tgcaaagaaa
     5701 aaacaaattg atagtttgtt gtctgaggct agagagtatg actttgccag tgtttgcgtt
     5761 aatccgacct gggttgaaca tgctaaaaaa ggacttgaag gcacagatgt taaggtttgc
     5821 acagtagtag gtttcccttt gggagcaaca acttcagccg tgaaagcatt tgagacaaaa
     5881 gaagctatcc aaaatggtgc agatgagatt gatatggtga tcaatgttgg agctctcaaa
     5941 tcaggtaatt tagccttggt tgagtcagat attcgcgcag tagtggaagc aagtggtgat
     6001 aagttagtga aagtcattat tgaatcttgc cttctgacag accaagaaaa aattgttgtt
     6061 tgccaattgg cccaaaaagc tggggctgac tttgttaaaa catctactgg cttttcaact
     6121 ggtggtgcta cgatagcaga tgttagatta atgcgtgaaa cagttggatc tgatatggga
     6181 gtcaaggctg ctggtggagc tcgttcttat gcagatgctc ttgcctttgt cgaagcaggt
     6241 gcgacccgta tcggaacgtc agctggggta gctattttaa aaggagaatt ggcagatggc
     6301 gactactgag ttgattgaac tggcaattga aaccagcaaa catgcctatg tcccctattc
     6361 tcactttcct atcggagcag ttttagtagc caaagacgga agtgtttaca cgggagtgaa
     6421 tatcgaaaat gctagctatc ctttgaccaa ttgcggtgag agaacagctg tttttaaggc
     6481 tatatctgaa ggccaaagag aattttcaga attgattgtc tatggtcaaa ctgaaaaacc
     6541 gattttacca tgtggtgctt gtcgccaagt aatggtcgaa ttttttgaac aagatttaaa
     6601 agtgacctta gtcgcaaaag ataaatcgac ggtcgagatg acggtcgggg agttacttcc
     6661 atactctttt acagacttaa attagtctga gtcgctcttt gagtggcaag ggtctttgtg
     6721 accaatcaat ccatacttgc aacatcgttg cacatcttat ttaggaggtt cagtaatgaa
     6781 caagaaacaa tggctaggtc ttggcctagt tgcagtggca gcagttggac ttgctgcatg
     6841 tggtaaccgc tcttctcgta acgcagcttc atcttctgat gtgaagacaa aagcagcaat
     6901 cgtcactgat actggtggtg ttgatgacaa atcattcaac caatcagctt gggaaggttt
     6961 gcaagactgg ggtaaagaac acaatctttc aaaagataac ggtttcactt acttccaatc
     7021 aacaagtgaa gctgactacg ctaacaactt gcaacaagcg gctggaagtt acaacctaat
     7081 ctttggtgtt ggttttgccc ttcacaatgc agttgaagaa gcagctaaag aacacactga
     7141 cttgaactat gtcttgattg atgatgtgat taaagaccaa aaaaatgttg ctagcgtaac
     7201 tttcgctgat aatgagtcag gttaccttgc aggtgtggct gcagcaaaaa caactaagac
     7261 aaaacaagtt ggttttgtag gtggtatcga atctgaagtt atctctcgtt ttgaagcagg
     7321 attcaaggct ggtgttgcgt cagtagaccc atctatcaaa gtccaagttg actacgctgg
     7381 ttcatttggt gatgcggcta aaggtaaaac aattgcagcc gcacaatacg cagccggtgc
     7441 agatattgtt taccaagtag ctggtggtac aggtgcaggt gtctttgcag aggcaaaatc
     7501 tctcaacgaa agccgtcctg aaaatgaaaa agtttgggtt atcggtgttg atcgtgacca
     7561 agaagcagaa ggtaaataca cttctaaaga tggcaaagaa tcaaactttg ttcttgtatc
     7621 tactttgaaa caagttggta caactgtaaa agatatttct aacaaggcag aaaaaggaga
     7681 attccctggc ggtcaagtga tcgtttactc attgaaggat aaaggggttg acttggcagt
     7741 aacaaacctt tcagaagaag gtaaaaaagc tgtcgaagat gcaaaagcta aaatccttga
     7801 tggaagcgta aaagttcctg aaaaataatg gatggaagtc atttcttagg aagcggccaa
     7861 gggccgcttt tttaagagtt gagacaaagt cattttgtct cagtaaagct tgctactaga
     7921 caaactagca agttttattg aaataaaata agactctgaa aggaagagca catggcacac
     7981 gaaaatgtca ttgagatgcg tgatattacc aaggtgtttg gtggatttgt tgccaacgac
     8041 aaaatcaact tgcacctacg aaaaggtgaa attcatgcac ttttaggaga aaatggggct
     8101 ggtaagtcca cgctaatgaa catgttagca ggccttcttg aaccaactag tggtgaaatc
     8161 gcggtcaacg gtcaagttgt caatctcgac tccccatcta aagcagctag cttgggaatc
     8221 gggatggttc accagcactt tatgttggtt gaagccttca cagtggctga aaacatcatt
     8281 ttaggtagtg aattgactaa aaatggtgtg ctagatatcg ctggagctag caaagaaatc
     8341 aaggctcttt ctgaacgtta tggcttagct gttgaccctt ctgccaaggt agcagatatc
     8401 tcagttggag cccaacaacg tgtagaaatt ttaaaaacac tttatcgggg ggctgatatc
     8461 cttatctttg acgaaccaac ggctgttttg actccatcag aaattgatga gttgatggct
     8521 attatgaaaa atcttgtcaa agaaggaaaa tcaattatct tgattaccca caaattggat
     8581 gaaattcgag cagtttctga ccgtgttaca gttatccgtc gtgggaaatc aattgaaacc
     8641 gttgaaattg caggggctac caatgctgat ttggcggaaa tgatggtagg acgttctgtt
     8701 tcctttaaaa cagagaagca agcctctaaa ccaaaagaag tggttttgtc tatcaaagat
     8761 ttggtggtca atgaaaaccg tggtgttcca gctgttaaaa atctgtcctt ggatgttcgt
     8821 gctggagaga ttgttggtat tgcggggatt gatggaaatg gtcagtctga actgattcaa
     8881 gccattacag gtcttcgcaa ggttgaatct ggtagcattg agctaaaagg agattcaatt
     8941 gtaggcttgc acccacgtca gattacagaa ctaagtgttg ggcacgttcc agaagaccgt
     9001 caccgtgatg gcttgatttt ggaaatgatg atatctgaaa atattgccct tcaaacctac
     9061 tataaagaac cacatagtaa aaatggaatt ttgaattatt caaatattac ttcttatgct
     9121 aaaaagctga tggaagagtt tgatgttcgc gctgccagtg aattagttcc tgcagctgca
     9181 ctctcaggag gaaatcaaca aaaagcaatt attgctcgtg aaattgatcg agatcctgat
     9241 ctccttatcg ttagccagcc aactcgtggg ttggatgtcg gtgccattga gtatatccac
     9301 aaacgcttga ttgaagagcg tgataatggc aaggctgtcc ttgttgtcag ctttgaattg
     9361 gatgagattt taaacgtctc agaccgtatt gccgttatcc acgatggtaa gattcaaggt
     9421 attgtatcac cagaaacaac caataaacaa gaacttggtg tcttgatggc tggtggaaac
     9481 ttgggaaagg agaagagtga tgtctaaaaa attacaacaa atttcggttc ccttgatttc
     9541 tgtattccta ggaattttac tcggagccat tgtcatgtgg atcttcggtt atgatgctat
     9601 ttggggctac gaagaattgt tctatacagc ctttggcagt ctgcgtggga ttggagaaat
     9661 cttccgtgct atgggtcctc tggtcttgat tggtcttggt tttgccgttg ccagtcgagc
     9721 tggtttcttt aacgtcggac ttcctggtca ggctttggca ggttggattc tcagtggttg
     9781 gtttgccctg tcgcatccag atatgccccg tcccttgatg attctagcaa ccatcgtgat
     9841 tgccttgatt gctggtggga ttgtcggagc gattccaggt attcttaggg cctatctagg
     9901 gacgtcagag gttattgtaa ccatcatgat gaactacatt gtcttgtatg tagggaatgc
     9961 ctttatccat gctttcccta aagacttcat gcaaagtaca gattcgacca ttcgtgttgg
    10021 ggctaatgca acctatcaga caccttggtt ggctgagttg actggtaact cacggatgaa
    10081 tattggtatt ttctttgcca tcattgccgt tgcagttatt tggttcatgc tcaagaaaac
    10141 aactcttggt tttgaaatcc gtgcagttgg tcttaatcca catgcttcag aatatgctgg
    10201 tatttctgcc aagcggacta ttatcctatc tatgattatt tcaggtgcct tggcaggtct
    10261 tggtggagct gttgaaggtt tgggaacctt ccagaacgtc tatgttcaag gttcgtcatt
    10321 agctatcgga tttaacggaa tggcggttag tttgcttgcg gccaactcac caattggtat
    10381 actttttgca gccttcctat ttggcgttct ccaagttggg gctcctggta tgaatgcggc
    10441 gcaggtacca tctgagcttg tcagcattgt aacagcgtct attatcttct ttgtcagtgt
    10501 tcattacctt atcgaacgct ttgtcaaacc gaaaaaacaa gttaaaggag gtaagtaaag
    10561 atgtctatta taaccttgct ccccctcttg gtgtcttcta tgctgattta ctcagcacct
    10621 ctcatcttta caagtatcgg tggtgttttc tctgaacgtg gtggtgtggt aaacgttggc
    10681 cttgaaggaa ttatggttat gggtgccttt tctggagttg tctttaacct tgaatttgca
    10741 gaacaatttg gagcagcaac tccatggcta tccttgcttg tagcaggatt ggttggtagt
    10801 gttttctcta tcatccacgc agcagcgacg gttcatttcc gtgcagacca tgttgtcagc
    10861 ggtacggtat tgaacttgat ggcgcctgcc ttggctgttt tcttagttaa agttctttat
    10921 aacaaaggac aaaccgacaa cctaagtcaa acttttggac gctttgattt cccagtcttg
    10981 gcaaatatcc cagtgattgg tgatatcttc ttcaagtcaa ctagtctact tggttatctg
    11041 gcgattgcct tctcattcct tgcttggttt attctcttta agactcaatt tggtcttcgt
    11101 ctccgctctg tcggtgaaca tcctcaagca gcggatacct tgggaatcaa cgtctacaag
    11161 atgagatatt tagggattat tatttcaggt tttctaggtg gaattggcgg agcgatttat
    11221 gcgcaatcaa tctcagttaa cttctcagtg acaactattg ttggacctgg atttatcgcc
    11281 cttgctgcga tgatctttgg gaaatggaat ccaatcggtg ctatgctttc tagtctcttc
    11341 tttggacttt cacaaagttt ggctgttatc ggttctcaat tgccgttcct acaaggagtg
    11401 ccagcggttt atcttcaaat tgcaccttat gttttgacaa ttcttgtctt agcagccttc
    11461 tttggaaaag cagtcgcacc aaaagcagat ggtatcaact acatcaaatc aaaataagca
    11521 tacaaaaaaa cgtcagtcca atga
//
LOCUS       DAVAAL010000051        11510 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVAAL010000051 DAVAAL010000000
VERSION     DAVAAL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.052"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(103..417)
                     /gene="trxA"
                     /locus_tag="VQM60_001162"
     CDS             complement(103..417)
                     /gene="trxA"
                     /locus_tag="VQM60_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET1081034.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            complement(434..655)
                     /locus_tag="VQM60_001163"
     CDS             complement(434..655)
                     /locus_tag="VQM60_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET1081035.1"
                     /translation="MIEITYLDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            complement(754..1134)
                     /locus_tag="VQM60_001164"
     CDS             complement(754..1134)
                     /locus_tag="VQM60_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET1081036.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            complement(1277..2230)
                     /locus_tag="VQM60_001165"
     CDS             complement(1277..2230)
                     /locus_tag="VQM60_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET1081037.1"
                     /translation="MSISQRTTKLILATCLAYLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(2298..3506)
                     /gene="sstT"
                     /locus_tag="VQM60_001166"
                     /pseudo
     CDS             complement(2298..3506)
                     /gene="sstT"
                     /locus_tag="VQM60_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            3645..4205
                     /locus_tag="VQM60_001167"
     CDS             3645..4205
                     /locus_tag="VQM60_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET1081038.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            4262..5170
                     /locus_tag="VQM60_001168"
     CDS             4262..5170
                     /locus_tag="VQM60_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET1081039.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIG
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            5122..5712
                     /locus_tag="VQM60_001169"
     CDS             5122..5712
                     /locus_tag="VQM60_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET1081040.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            5715..6821
                     /gene="yqeH"
                     /locus_tag="VQM60_001170"
     CDS             5715..6821
                     /gene="yqeH"
                     /locus_tag="VQM60_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET1081041.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVIRKAII"
     gene            7035..7346
                     /gene="yhbY"
                     /locus_tag="VQM60_001171"
     CDS             7035..7346
                     /gene="yhbY"
                     /locus_tag="VQM60_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET1081042.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            7398..8027
                     /locus_tag="VQM60_001172"
     CDS             7398..8027
                     /locus_tag="VQM60_001172"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET1081043.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            8027..8620
                     /gene="yqeK"
                     /locus_tag="VQM60_001173"
     CDS             8027..8620
                     /gene="yqeK"
                     /locus_tag="VQM60_001173"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET1081044.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            8621..9121
                     /locus_tag="VQM60_001174"
     CDS             8621..9121
                     /locus_tag="VQM60_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET1081045.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            9134..9487
                     /gene="rsfS"
                     /locus_tag="VQM60_001175"
     CDS             9134..9487
                     /gene="rsfS"
                     /locus_tag="VQM60_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET1081046.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            9590..10336
                     /locus_tag="VQM60_001176"
     CDS             9590..10336
                     /locus_tag="VQM60_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET1081047.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKITFIEGNMLNLSNAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            10346..11443
                     /locus_tag="VQM60_001177"
     CDS             10346..11443
                     /locus_tag="VQM60_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET1081048.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGTEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARENVLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
BASE COUNT         3442 a         2263 c         2621 g         3184 t
ORIGIN      
        1 gtactattct agtttcaatc tactatagtc tagcatattt tttgaaaaat ggcaaagggc
       61 aagaaaaaag agaccaaaga aagtacttgg tctctcgttt gattagctca attcagcaat
      121 gatggccttg atttgttctg ctgtgtgaac acctgcaact tgtttgacaa cttggccgtc
      181 ttttttgaag agaagagttg gaatagacat gattccaaaa gcacgagctg tgtttggatt
      241 ttcatcaacg tccattttaa cgattttcaa gacatcttct gaaagttctt cagacaattt
      301 gtccaagatt ggaccttgca tacgacatgg accacaccaa gttgcccaga agtctactaa
      361 gaccaaaccg tcttttgttt cttgttcgaa tgttgcatct gtaattgctt ttgccattgt
      421 atttctcctt tttttagtta tattggctta aatcttgttt catgagatag aagaagatat
      481 ctccataagt cccatggtag tccaaattat gacccttgta agttaatttt tggacagggt
      541 agtagtctgc gacgccgata aggcaagctt gttgcgaacg ttcaaagtct tcataagact
      601 cgaaagtcac agttctttcg ttcttgctgg catctaaata ggtaatttca atcatattta
      661 aaactccttt gtttaatgct aactttattt tactccttat aaaagagaat gtcaagaaaa
      721 atgattgcgc acgcaacttt ttttaaaatc atcttaaatc aagaaatcca aacctgcttc
      781 caagctttct tcgacagtct tttgtagcga ggccagtgtc ttttgcccat catttgtcag
      841 gcagataaaa ctagagcgtc tatcttgatg gcaacacatg cgactgagta gaccgcaatt
      901 tttagcttcc aagcgagcca ccatcctaga aactgcgctc gggctcagat gaagcttatc
      961 tggcaggtca atctggcgta gagatttttc ttcagctaag tccagatagt agagcaggta
     1021 gaactctttc aaggtcagac tttgctcgct ctgttgggca atggtctctt ccaagagact
     1081 ttcaatttct ttctgacgcc gattgaagtc aaaccatttt tccaaatagg tcatagtatc
     1141 tcctttcttt ttagagtcat aaatagaaga aagtccatta acgggcagtc tctgcgtcat
     1201 aagatgattg cgcatgcaat aattatacta cttttcaaga atgctggcaa gctctgtttt
     1261 ttagtggttt tcttttttac tgtctatatt tttggtaaaa atcaactttt acttggatga
     1321 aggttttggc ttcacgtagg agttgaagaa gggtggcgcg ggtttcaaat tcttctcttg
     1381 tcttgggcag actgcggttc cggaagactt ccagataacg ttcaatttca tctagcaaat
     1441 cagaagcagg attggtctgg ctcagttgac ctgcaatttt tgaaaagagt tgcgctaaga
     1501 tcaggctttc actggcggca aggtgacaag tgttaatctg ttgggccatg tttctcagga
     1561 tacgactttg tcgctgtctc atctcaaagt agtggatatg gtagtcggtc tggtgaaaga
     1621 ggtggtcaga gtggtccaaa tagaccagtc tgagggcttc tttcaaaagc gtgtctaatt
     1681 ctgctaccag ctgtgctcgg ttgcgtccgt ctcctctgga taaatagtat ttgaagcgct
     1741 ggaggatatc ttttaacttt tcttccacca gcgtgtggta gtgctggatt tcctcttctc
     1801 gtgaaggcat atagagatta acaagcaagg caaatcctgt accaatagca aagagaagga
     1861 attcattgac tagaaggtct ggagaggttg actcttgaac caagagatgg ctaaccaaaa
     1921 cagtgcttgg tgtgatgcca atttcccagc ccatcttgta ggctaaagga acgtagaagg
     1981 ccagatagag gccgagactc cagatatgaa atccgctcaa gtgaaaagct agaacaccga
     2041 tagccagagc tagaagcata gaaaaaagac gattgcgagc cagttttaaa gtacttctac
     2101 gcgtatcaga taggctcaag agagcgataa ttccagccga aactgctgat gaaagattga
     2161 gaaaataagc aagcaggtag gcaagacagg tagctaagat gagcttggtc gtacgttggc
     2221 taatagacat aagaatttcc taataagtta gaataaaagc gtaaaagaca agacatgagc
     2281 aggcttgcct tgatgagtta ttttttacgg gttgctgcgt attcggcaac ggcggtaaag
     2341 aggacatctg tagaagagtt aagggctgtt tcacatgagt cttggatgac accaatcaca
     2401 aaaccaaccc caacaacttg tatggcaata tcgttagaaa taccgaaaag gctacaagca
     2461 actgggataa gaaggaggga acctccggca atacctgaag caccacaggc tgagatagct
     2521 gctaccacac tgaggacaaa ggctgtggca aagtcaacag gaattccaag agtgttaact
     2581 gcagcaaggg tcaaaacgtt aatggtaatc gctactccag ccatattgat agtagaaccg
     2641 agtgggatag aaacagaata ggtatctggg ttgagtccaa ggtcatggca gagtttcatg
     2701 ttgacaggaa tgttagccgc agaactacga gtgaaaaagg ctgtcacacc gctgacacgg
     2761 aggcagttcc aaactagagg gtaaggatta cgtctcataa agaagaaggc aatcaaaggg
     2821 tgaccacaag ggcaacaaaa agcatagtcg ttactaatag aaccaataaa ataccgtagt
     2881 tggcaaggct tccgactccc ttgtcagaaa tggttttaaa aacaagacca aggattccaa
     2941 atggagccag attgatgatc cattcgacaa ttttagaagt cacgtcagcg atagttttta
     3001 gcaattcttt actattttta ctggcttctc tcatagcgat tccaaaaatg actgcccaag
     3061 ataagattcc aatatagtta gcagtaagca gggcgttgac tgggctgtca accagtttga
     3121 gcaagaggtt gctgaggacc tgcccaatcc catctggtgg tgcaatttca gtattggcac
     3181 tatttagggt aatttcaata gggacgatga aacttgctag tacagctaca agagcagcgg
     3241 cgaaagtccc tatcatagga tatacaagaa aacaacagtt ttcatattgc tatcttgtcc
     3301 cttttgatgt tgggaaaggg cattggcaac gagggcaaag actaggatag gagcaacagc
     3361 ttttagacct ccaacgaata aatccccgag tagcccaatc cctgagagat taggaagggt
     3421 cagtcctagg attccccata aagcatacca atcaagatac gcttgacaag gcttgcctta
     3481 ttccaagcat gaatgattct tttcataata atcttctttt tgtgtagtga ttatgattat
     3541 agtataaatg atagacaaaa tcaagaattt tctgtctatt ttttgaatat ttatggagaa
     3601 tgagactgat gaaaatatgg tataatgaaa taaaggagtt ttatatgcaa aaatttattc
     3661 aggcttatat tgaaaagcta gatgtgacaa ccattatcga gaatattcta accaaggtca
     3721 tttctctttt actgcttttg attgtatttt atattgctaa aaaaatgctt cataccatgg
     3781 tgcagagaat tgtcaaacct tctctaaaaa tgtctcgtca tgatgtcgga cgccaaaaaa
     3841 ccatctcacg tttactagaa aatgtgttta attatacgct atatttcttt ttactctact
     3901 gcattttgtc gattttaggt ttgccagttt ctagtttgct ggctggagct ggtattgctg
     3961 gggtagcgat tggtatggga gcccaaggct ttctgtctga tgtcatcaat ggctttttta
     4021 tcctctttga acgtcaactg gatgtgggag atgaggtcgt tctgacaaat ggaccgatta
     4081 ctgtatcggg taaggttgtc agtgtgggaa ttcgtacgac acagcttcgt agcgaggagc
     4141 aagcccttca ctttgtccct aaccgaaata tcacagttgt tagcaatttc tcacgcacag
     4201 actagacctg ttattttaag taatttgtgg tacaatagag ggagtttaat aaggagaaaa
     4261 gatggtttta gaaaagcagt tgggcaatgg ttgtacctgg atagacctag acctaggaaa
     4321 gttgaataaa ctagaagacc tttctgaaat ttacggtttg gacaaggaaa ccattgaata
     4381 cgcactggat agaaacgagc gcgcccacat ggactaccac cgtgaaagtg ggacggttac
     4441 ctttatctat aatgtcctag acgtaaaaaa ggacaaggcc tactatgaga cttttcccat
     4501 gacctttatc gtcgagcatc gtcgcctgat taccattagt aataccaaga acgcctatgt
     4561 cattggacag atgactcgtt atctggagag ccatgacacg ctttcgattt ataagtttct
     4621 ctttgccagt ctggaaatca tcagcaatgc ctactatcct gtcattgagc agatggacaa
     4681 gagtagggat gaggtcaatg accttttgcg ccagcgaact accaagaaaa acctctttgc
     4741 cctgtctgat ttggagactg gtatggttta tctgacggca gctgccaaac aaaatcggat
     4801 tttgttagag catattcaag gtcatgcctt gtatcgtagt tttgatgaga ttgagagaga
     4861 acagtttgat gatgccatga ttgaggctca tcagctggta tccatgacag acctaatctc
     4921 tcagatttta cagcagcttt cagcctctta caacaatatt ctaaacaata atctgaatga
     4981 caatttgaca accttgacta tcatttcagt cttgctagct gttttggcag tcgtgacagg
     5041 ctttttcgga atgaatgttc ccttaccttt aacagatgag ccccatgctt ggctctatat
     5101 cagtttggct agtgcaggtt tgtggattgt tttatccttg ttactaagga aaattgcgaa
     5161 aaaaagttaa gaaaaggagc cagaatggcg attgaaaatt atataccaga ttttgctgtg
     5221 gaagcagtct atgatctgac agtcccaagc ctgcaggcgc agggcatcaa ggctgttttg
     5281 gtcgatttgg ataataccct cattgcttgg aacaaccctg atggaacgcc agagatgaag
     5341 caatggctac atgaccttcg ggacgcgggt attggcatta tcgtagtgtc aaataacacc
     5401 aaaaaacgcg ttcaacgagc agttgagaaa tttgggattg attacgttta ctgggccttg
     5461 aagcccttca catttggtat tgaccgtgct atgaaggaat tccactatga caaaaaggaa
     5521 gtggtcatgg ttggtgacca gctcatgaca gatatacgag cagcccaccg tgcagggatt
     5581 cggtcaattt tagtcaaacc cttggtccaa catgactcaa tcaaaacgca gattaaccga
     5641 actcgtgagc gtcgtgttat gagaaaaatc actgaaaagt acggaccgat tacatataaa
     5701 aaaggaattt aactatggaa gaaattctct gtattggttg tggagcaacc attcagacga
     5761 cagataaagc tggtcttggt tttacccccc agtcggcact tgaaaaaggt ttggagactg
     5821 gcgaagtcta ttgccaacgc tgtttccgtc tccgccacta caatgaaatc acagatgtcc
     5881 agttgacgaa cgatgatttc ctcaagctct tgcacgaggt gggagacagt gatgctttag
     5941 tggtcaatgt cattgatatc tttgatttta atggatctgt catcccaggt ttaccacgtt
     6001 tcgtctcggg caatgatgtc ctcttggtag gaaataaaaa agatatcctt cctaagtcag
     6061 ttaagtctgg taagattagc cagtggctca tgaaacgtgc ccatgaagaa ggtcttcgtc
     6121 cagtcgatgt ggtcctaact tcagcacaaa ataaacatgc cattaaggaa gtcattgaca
     6181 agattgaaca ctaccgtaag ggccgcgatg tctatgtggt cggtgtgacc aacgttggaa
     6241 aatcaactct aatcaatgct attatccaag aaatcacggg tgatcagaat gtcatcacta
     6301 cttcacgctt cccagggaca accttggaca aaatagagat tccgcttgac gacggatctt
     6361 atatttacga tacgccggga attatccacc gccaccagat ggctcactac ttgacggcca
     6421 aaaacctcaa gtatgtcagt cctaaaaagg aaatcaagcc taagacctat cagcttaatc
     6481 ctgagcaaac cctattttta ggtggtttgg gacgctttga ctttatagca ggagaaaagc
     6541 aaggatttac tgctttcttt gataatgaac tcaaactcca tcgtagcaag cttgaaggag
     6601 ctagtgcttt ctacgataag cacctgggaa ctcttctgac accaccaaat agcaaggaaa
     6661 aagaagattt cccaaggcta gtccagcatg tctttaccat taaagataag acagacctag
     6721 tcatctcagg cctaggctgg attcgtgtaa caggcatagc aaaagtcgcc gtctgggcac
     6781 cagaaggcgt cgccgtcgtc atacgaaaag caattattta aaacaaaatc gctgagcgaa
     6841 caaagtgagc tcatatagcg atagttattg ccgtggtgtg attgccaatc tgtagattgg
     6901 caaggggcac aattgagacc aaggctcaat tgtgaggtca ggaaatccat ttttcaaaga
     6961 tgaaatcttt ggaaaattag ttccgaccgt ccctcaccac ctaaaataac tatcaaaaaa
     7021 caaggaagaa aattatgtca ttaacatcaa aacaacgtgc cttcctcaac agccaggcac
     7081 acaccctcaa acctatcatc caaatcggga aaaatggact caacgaccaa atcaaaacca
     7141 gcgtccgtca agctcttgat gcgcgtgaat taatcaaggt tactctctta caaaacacag
     7201 atgaaaacat ccacgaagta gctgaaattt tggaagaaga aatcggtgtg gatacagtcc
     7261 aaaaaatagg acgcatcttg attttgttta aacaatctag caagaaagaa aatcgcaaga
     7321 tttctaagaa agtcaaagaa atctaagacc gaaactccaa ataactgttt ttatagagaa
     7381 ataaagggga ctagcctatg acaatcgaac tattgactcc ctttaccaag gtagagttgg
     7441 agccagaaat caaggagaaa aaacgcaaac aagttgggat tttagggggg aattttaacc
     7501 ctgttcacaa tgcccatctc attgttgcgg atcaagtacg gcaacagttg ggactggatc
     7561 aagttcttct catgcctgaa taccaacctc ctcacgttga taaaaaggaa accatccctg
     7621 aacaccatcg tctcaagatg cttgagttgg caattgaggg gattgacggc ctagtcattg
     7681 aaaccattga gttggagcgc aagggtattt cctacaccta cgacaccatg aaaatcttga
     7741 cagagcaaca tccagatacg gattattact ttatcatcgg cgccgacatg gttgactatc
     7801 tgcctaagtg gtaccgaatt gatgaactgg ttgacatggt tcagtttgtg ggggttcagc
     7861 gtccacgcta caaggcaggg acttcctatc cagttatctg ggtggacgta ccgctcatgg
     7921 atatctcgtc cagcatggtg cgggacttca ttgcccaagg tcggaaaccc aactttctcc
     7981 tacctcagcc agtgctagac tacatcgaga aggaggggct ctactgatgt cctatcaaga
     8041 ctatatcaac tgctcccgtg aggccttgtt ggaaaaaatg gcagagcttc tacctgagaa
     8101 acgtctaacc cattgtctag gtgtggagcg tgctgctatg gaattagctc agcgctttgg
     8161 tgtagatgtt gagaaagcaa gtctagcagg tcttcttcat gactatgcta aaaagttgtc
     8221 agatcaggaa tttctagact tgattgaccg ttaccagcta gaccctgacc tcaaaaactg
     8281 gggcaataat gtctggcatg gtatggtcgg aatttacaaa attcaggaag atttggattt
     8341 gcatgattct gaaattctgc gagccattga aattcataca gtcggagctg gtcaaatgac
     8401 agacctagat aaggtcatct acgtcgcaga ctatatcgag cacaatcggg catttcctgg
     8461 agtggatgtg gcgcgtgaga ttgcaagtct atcgctcaat aaggcggtgg cctacgaaac
     8521 agctcgtacc gtggagtatc tagctcatca gggattcccc atctatcccc aaacccttga
     8581 aacctataac gcctttgtgc actatttgaa agagaactaa atgaagacgg cttttataat
     8641 cattgatgtt cagaatgttt tagtagaaac tgggttccag acaaaaagtc tattgggaaa
     8701 aatttcttat ttacaaaacc aggctagaag caagaatatt gaaattatct atgttcaaca
     8761 tattgagaac tctgaagctc aaacatcaga agattggcag ttatctgcgc ttttaaatcg
     8821 aaaacctgct gaaaaggtct ttcagaagaa atataacagt attttcaaag aaactggctt
     8881 aaaagaatac ttggataagc aggggattga aaaattagtt ttatgtggta tgcagacaga
     8941 atattgtgtg gatacctctg tcaaggttgc ctttgaatat ggctatcagc ttattgttcc
     9001 agaaggtgct gtcacaacct ttgatgggga tgacattcca gcagaaacga ttaatgaatt
     9061 ttatgaggac atttgggagg agcgctttgc agatgtccta gattacaaac atattttcta
     9121 aaaagaggat taaatgaacg aaaaagaatt actagaacta gtcgtgaaag cggctgatga
     9181 gaaacgtgcg gaggatatcc tcgcacttga tgtacaagat ttgactagtg tgacggacta
     9241 ctttgtcatc actagctcaa tgaatagccg tcagttggac gctatcgcag ctaatatccg
     9301 tgaaaaagta gctcaagcag gctttaaagg tagccatgtc gaaggcgatg cagctggagg
     9361 ctgggtctta ctggacctcg gtgctgttgt cgtgcatatc ttttcagaag aaatgcgtgc
     9421 ccattataat ctagagaagc tatggcatga ggcgaattca gtagatattt cagaagctct
     9481 tgcttagaag atgaactcag ttgtagtcaa ctgggtttct ttgcttattt tagaaaaatt
     9541 gatagaaagg taaagggcta gtggtgttta ccatggagct cttagtatca tgattatggc
     9601 gacttatgaa acctttgcgg cggtctatga tgcggtcatg gacgatagtt tatacgacaa
     9661 atggacgaat ttttctctgc gtcatttgcc taagaccaag gagagaaaga aactcttgga
     9721 attggcttgt gggacaggaa ttcaatcagt gcgcttctct caggctggtt ttgatgtgac
     9781 tggacttgac ttgagtgcgg atatgttgaa gattgcggag aagagagcaa cttcagccaa
     9841 gcaaaagatt acttttatag aaggtaatat gctgaatttg tccaatgcag ggaaatacga
     9901 ttttgtcacg tgttattcgg actctatctg ctatatgcag gatgaggtgg aagtagggga
     9961 cgtctttaag gacgtgtaca atgcgctgaa tgaagaggga gttttcatct ttgacgtgca
    10021 ctcgacctac cagacagatg aagtattccc tggctattcc taccatgaaa atgcggaaga
    10081 ttttgccatg ctttgggata catatgagga cgaagctcct cactccatcg tgcatgagct
    10141 gactttcttt atcaaggagg ctgacggttc ctttagtcgt catgatgaag tacatgagga
    10201 gaggacctat gagatcttga cctatgatat cttgctggaa caggctggat ttaagtcttt
    10261 caaactctat gcagactttg aggataagga gccaacagaa actagcaccc gttggttttt
    10321 tgtggcgcag aagtaggaga acatcatgac catcacaggt attatcgcag agttcaatcc
    10381 ttttcataat ggccataaat acctgctgga tcaggcagag ggactgaaaa ttgttgccat
    10441 gtcagggaat ttcatgcagc gtggagagcc tgctattgtg gataagtgga caagggccca
    10501 gatggcgctg gaaaatggcg cagacttggt agtggaattg ccctttttag tcagtgttca
    10561 ggcagctgat ttctttggtc aaggagctgt ggatatcttg gatcgcttgg gcattgatag
    10621 tctagttttt gggacagagg aagttcgaga ttatcagaaa atcgctgact tatacacaga
    10681 gaaaggtact gagatggaga aatttgtgga aaatctgcct gattcccttt cctatcccca
    10741 gaaaacccaa gctatgtgga aggaatttgc tggtcttgat ttttcaggta atacccccaa
    10801 tcatgttctt gctctggcct atgccaaggc ggttgcagga cggaatatca agctgcatcc
    10861 gattcagcgt cagggggcag gttaccattc tgtgaataaa gatgtggact ttgcctcggc
    10921 gacagccctc cgtcaacatc agaaggacca agatttctta gaacgtttta tgccttctgt
    10981 tgccctattt gagcaggcca gtaaggtgat ctgggaagac tattttccct tgctccgcta
    11041 tcaaatcttg tcaaatcccg acttaaccac catctatcag gtcaatcaag aaatggctgt
    11101 gcgcattaaa gaagctatta aaacagccca gtctgtggaa gaattggtcg agttagtgac
    11161 taccaaacgt tacaccaagg cgcgtgtcag acgcctattg acctatattt tgatgcaggc
    11221 tagagaaaat gtcttgccag aagccattca tgtccttgga tttactgaaa aaggtaggca
    11281 acatctcaag tctttgaaag gtcaggtcag tctagtcagc cgaattggca aagaaccttg
    11341 ggatgctatg acccaaaaag cagaccagat ttaccaacta ggaaagccaa gtatagcaga
    11401 gcaaaatttt ggaagagtgc cgattagaat agaaacaaac taagtctacc gaaaggtgga
    11461 ctttttgaga ttttttacga ataatactct tcgaaaatca aattcaaatc
//
LOCUS       DAVAAL010000052        11384 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVAAL010000052 DAVAAL010000000
VERSION     DAVAAL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.053"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            120..857
                     /locus_tag="VQM60_001178"
     CDS             120..857
                     /locus_tag="VQM60_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1081049.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPYLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            854..2545
                     /locus_tag="VQM60_001179"
     CDS             854..2545
                     /locus_tag="VQM60_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET1081050.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            2661..3224
                     /locus_tag="VQM60_001180"
     CDS             2661..3224
                     /locus_tag="VQM60_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET1081051.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            3246..8954
                     /locus_tag="VQM60_001181"
     CDS             3246..8954
                     /locus_tag="VQM60_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET1081052.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVEAEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKGPEQPVQPTQAEQPRIPKDSSQPEDPKEDRGAEETPKQEDEQPAEAPE
                     IKVEEPVESKEETKTAKGTQEEGKEGQAPVQEVNPEYKVTTGTVEKSTESELDFTTEV
                     VPDDTKYVDEEVVERQGSKGVQVTKTTYETVEVVETDKVLSTTTEVKTPAVPKVVKKG
                     TKPVKGTAVETREEVIPFATKEQEDDTLKRGTRQVAQEGVNGKKQITETYKTIRGEKT
                     NEAPTVEETVLQAPQDEIIKKGTKGLEKPTLEWVKTDKDVLKKSATASYTLNKPDGVE
                     IKSIKVALKDNTGTVIKEVTVEENNLNATLDNLKYYQGYTLSTTMVYNRGEGEETETL
                     EDKEVQLDLKKVEIKNIKETSLMSVDDAGVETDKSLLTEKPTDVAPLYLRVTTHDNKT
                     TRLAVSSVEEVVVDGKTLYKVVAKAPDLVQRREDDTLSEEYVHYFEKQLPKVDNVYYS
                     FNELITDMQKNPTGTFKLGADLNAANTPTPSKSYVTGEFKGKLSSVDGQHYTIHNTAR
                     PLFNNIVGGTVKNINLNNVNIDMPWADQIASVANVIKGGSLIENVKVKGNVLGRNWVS
                     GFIDKIDSGGRLVNVAFIGNVTSVGDGDKFLTGIVGENWKGYVEKAYVEANIKGKRAK
                     AAGISYWSQNSGDNNAVGRDGAIKKSVVKGSIDVEKPIEVGGAVGSLNYLGYIEDTVA
                     MMKVKNGEIFYGSHDIDTDPYYTGERVNRNFIVDGVSEGKSSYTYSKQQKRIKSISQE
                     EADKKIKELGITADKFTIIDPIVNKLNNIKQKADTYKDTQDYKADRELTYRNIEKLQP
                     FYNKEWIVDQGNKVPSNSKLLTTEVLSVTGIKSGQFVTDLSDIDKIMIHYSDGAKEEL
                     NVTRQESNVQQVREYSITDLDILYTPNMVEKDRAQLMTDVKSKLSSVELESDGVRQLL
                     VKRDTKKDANANSVGRQNGYIRDLFLEESFSEVKANLDKLVKQILENEDHQLNDNELA
                     ERALLKKVEDNKAKIMMGLAYLNQYYAFKYDELSIKDIMMFKPDFYGKTASVIDRLIN
                     IGSAENNLKGDRTQDAYRGIISGATGKGSLHDFLTYNMKLFTNETDINVWFKKAIEKN
                     AYVVEQPSTNPAFANKKYRLYEGINNGQHGRMILPLLNLKNAHLFMISTYNTISFSSF
                     EKYGKDTDEKREKFKSEINKRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDN
                     HNSPNGWASRHGHIDGKPDYAPIREFFGRINKYHGYKYGYGAYAYIFAAPQPMDAVYF
                     VMTDLISDFGTSAFTHETTHVNDRMAYYGGHWHREGTDLEAFAQGMLQTPSVSNPNGE
                     YGALGLNMAYERQNDGNQWYNTNPNDLTTRAEIDNYMKGFNDTLMLLDYLEGEAVLNK
                     ANQDLNNAWFKKVDKQLRGASTKNQYDKVRDLTADEKNITLNSVDDLVDNNFMTKHGP
                     GNNVYDPTGFGTAYVTVPITAGIYGGNTSEGAPGSMSFKHNTFRMWGYYGYEKGFLNY
                     ASNMLKNESRQAGHNTLGDDFIIKKVSDNKFSTLEDWKKAYFKEVVDKAKAGFNPVTI
                     DGTTYSSYDDLKNAFAAAVEKDKATLKNGSVKSDNTVALKEKIYKKLLQQTDSFKTSI
                     FR"
     gene            9015..10736
                     /gene="pabB"
                     /locus_tag="VQM60_001182"
     CDS             9015..10736
                     /gene="pabB"
                     /locus_tag="VQM60_001182"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET1081053.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLTVHKVPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKDQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            10737..11354
                     /locus_tag="VQM60_001183"
     CDS             10737..11354
                     /locus_tag="VQM60_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET1081054.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
BASE COUNT         3793 a         2004 c         2529 g         3058 t
ORIGIN      
        1 aacctgtggc tatcttccta gtttactttt tgatttttat tgagtataag aggtctttcc
       61 taagcagtta gaggagaatt ttgctatact gatactagta agtggtaaag gagcagagca
      121 tgacctacac aatcttaatc gtagaagatg aatatctggt aagacaaggt ttgactaaac
      181 tggtcaatgt agcagcctac gatatggaaa tcatcggtca ggctgaaaat ggaaggcagg
      241 cttgggaatt gatccaaaag caggtgccag atatcatttt aaccgatatc aacatgcctt
      301 atctaaatgg catccagttg gccagtctgg tacgagaaac ctatcctcag gttcatttgg
      361 tctttttaac aggttacgat gattttgatt atgccttgtc tgctgtcaaa ctaggtgtgg
      421 acgactacct gctcaaaccc ttttctcgtc aggatattga ggaaatgttg gggaaaatca
      481 aacaaaaact agacaaggaa gagaaagaag agcagttaca agatttattg actaataggt
      541 ttgaaggaaa catggcccag aaaatccagt ctcatctggc tgatagccaa tttagtttaa
      601 agtctttagc cagtgactta ggttttagtc cgacctatct gagttccttg attaagaaag
      661 agttgggctt gccttttcag gattatctgg tgagagaacg tgttaaacaa gccaagctct
      721 tgcttttaac tacagatctg aagatttatg agatcgcaga gaaggttggt tttgaagata
      781 tgaactattt tacccaacgt tttaagcaga ttgcaggtgt gacacctcgt cagtttaaga
      841 agggagaaga ccgatgaagc gttcttctct tttagttaga atggttattt ccatctttct
      901 ggtctttctc attctcctag ctctggttgg aactttctac tatcaatcaa gttcttcagc
      961 cattgaggcc accattgagg gcaacagcca aacgaccatc agccagacta gccactttat
     1021 tcagtcttat atcaaaaaac tagaaaccac ctcgactggt ttgacccagc agacggatgt
     1081 tctggcctat gctgagaatc ccagtcaaga caaggtcgag ggaatccgag atttgttttt
     1141 gaccatcttg aagtcagata aggacttgaa aactgttgtg ctggtgacca aatctggtca
     1201 ggtcatttct acagatgaca gtgtgcagat gaaaacttcc tctgatatga tggctgagga
     1261 ttggtaccaa aaggccattc atcagggagc tatgcctgtt ttgactccag ctcgtaaatc
     1321 agatagtcag tgggtcattt ctgtcactca agaacttgtt gatgcaaagg gagccaatct
     1381 tggtgtgctt cgtttggata tttcttatga aactctggaa gcctatctca atcaactcca
     1441 gttggggcag cagggctttg ccttcattat caatgaaaac catgaatttg tctaccatcc
     1501 tcaacacaca gtttatagtt cgtctagcaa aatggaggct atgaaaccct acatcgatac
     1561 aggtcagggt tatactcctg gtcacaaatc ctacgtcagt caagagaaga ttgcaggaac
     1621 tgattggacg gtgcttggcg tgtcatcatt ggaaaagtta gaccaggttc ggagtcagct
     1681 cttgtggacc ttgcttgggg ccagtgtcac atctcttctt gtctgtctct gcttagtgtg
     1741 gttcagtctt aaacgctgga ttgctccttt gaaggatttg agagaaacca tgttggaaat
     1801 tgcttctggt gctcaaaatc ttcgtgccaa ggaagttggt gcctatgaac tgagagaagt
     1861 aactcgccaa tttaatgcta tgttggatca gattgatcag ttgatggtag ctattcgtag
     1921 ccaggaagaa acgacccgtc agtaccaact tcaagccctt tcgagccaga ttaatccaca
     1981 tttcctctat aacactttgg acaccatcat ctggatggct gaatttcatg atagtcagcg
     2041 agtggtgcag gtgaccaagt ccttggcaac ctatttccgc ttggcgctca atcaaggcaa
     2101 ggacttgatt tgtctctctg acgaaatcaa tcatgtccgc cagtatctct ttatccagaa
     2161 acaacgctat ggagataagc tggaatacga aattaatgaa aatgttgcct ttgataattt
     2221 agtcttaccc aagctggtcc tacaacccct tgtagaaaat gctctttacc atggcattaa
     2281 ggaaaaggaa ggtcagggcc atattaaact ttctgtccag aaacaggatt cgggattggt
     2341 catccgtatt gaggatgatg gcgttggctt ccaagatgct ggtgatagta gtcaaagtca
     2401 actcaaacgt gggggagttg gtcttcaaaa tgtcgatcaa cggctcaaac ttcattttgg
     2461 agccaattac cagatgaaga ttgattctag accccaaaaa ggaacgaaag ttgaaatata
     2521 tataaataga atagaaacta gctaactccc agtcaattct gggagtttta tgcgtaattg
     2581 ggcaaacttt ctaggttgtc tatcttgcta aaaacaagaa aaaacggtat gatagaagag
     2641 tgacaacaga ggtaacagag atgaacgaga cacagattca aagagaaaca cgtcaggtag
     2701 tggaagatgt tttagaaaag accaatttga agcagggggc cctttttgtt ttaggtctat
     2761 cttctagtga ggtgctaggt ggtcagattg ggaaagaatc cagtcaagag attggtgagc
     2821 tgatagtgga gactatcttg ggtattctag gtagtagagg tatccattta gcagttcaag
     2881 gttgtgagca tgtcaatcgg gctctcgttg ttgaacgtca ggtggcagag cagtttggtc
     2941 tggaaattgt cagtgtccat cctactcttc atgcaggagg ttcggggcag ttggcagcct
     3001 ttaagtttat gcaggatcca gttgaggttg aatttatcaa ggctcatgct ggattggata
     3061 tcggagacac tgcaattggc atgcatgtca agcatgttca ggttccgatt cgccctattt
     3121 tgagagagat tggccatgcc catgtaacgg cactggctag tcgtccaaaa ttaatcggag
     3181 gtgcgcgtgc gcactatccg caagatgcta ttaggaagtc ttgagaatga ataaaggaga
     3241 taaaagtgtt taaaaaagac cgtttttcaa ttcgtaagat taagggagtt gtaggctctg
     3301 tatttcttgg aagccttttg atggctcctt ctgtagtgga cgcagccacc tatcactatg
     3361 taaataaaga gattatttca caagaagcta aagatttaat tcagacagga aagcctgaca
     3421 ggaatgaagt tgtatatggt ttggtgtatc aaaaagatca gttgcctcaa acagggacag
     3481 aagcatctgt tttgacagct tttggtttgc tgactgttgg gagcttgctt ttaatctaca
     3541 agagaaagaa aattgctagc gtctttctag ttggagctat gggattggta gttcttccta
     3601 gtgcaggggc tgtagaccca gttgcgaccc tagcactggc tagtcgagag ggtgttgttg
     3661 aaatggaggg ctatcgctat gttggttatc tatcaggtga catcctcaaa acgcttggct
     3721 tggacactgt tttagaagaa acctcagcaa aacctggaga ggtgactgta gtcgaagttg
     3781 agactcctca atcaacaaca aatcaggagc aagctaggac agaaaaccaa gtagtagagg
     3841 cagaggaagc tccaaaagaa gaagcaccta aaacagaaga aagtccaaag gaagaaccaa
     3901 aatcggaggt aaaacctact gacgacaccc ttcctaaagt agaagagggg aaagaagatt
     3961 cagcagaacc agctccagtt gaagaagtag gtggagaagt tgagtcaaaa ccagaggaaa
     4021 aagtagcagt taagccagaa agtcaaccat cagacaaacc agctgaggaa tcaaaagttg
     4081 aaccaccagt agaacaagca aaaggcccag aacaacccgt gcaacctaca caagctgagc
     4141 aaccaaggat accaaaagat tcatcacaac cagaagatcc taaagaagat aggggagcgg
     4201 aagagacacc gaaacaagaa gatgaacagc cagcagaagc cccagaaatc aaggttgaag
     4261 aaccagtaga atcaaaagag gagactaaaa cagctaaggg gacgcaagaa gaaggcaaag
     4321 aaggtcaagc accagtacaa gaagtaaacc cagaatataa agtaacaaca ggtacagttg
     4381 agaagtctac cgaaagtgaa ttagatttca caacagaagt agttccggat gacacaaaat
     4441 atgtagatga agaagttgtt gaacgtcaag ggtctaaagg agttcaagtt acgaaaacaa
     4501 cttacgaaac agtggaagtt gtagagacag acaaagtcct ttctacaact acagaagtga
     4561 aaactcctgc tgttcctaaa gtcgttaaaa aaggaacaaa accagttaaa ggaacagcag
     4621 tcgaaacaag agaagaagtc attccttttg ctacgaaaga acaagaagat gacactttaa
     4681 aacgcggaac aagacaagta gctcaagaag gtgtaaatgg taagaaacaa attactgaaa
     4741 cctataaaac aattcgcgga gaaaaaacaa acgaagctcc aacagtagaa gaaacagttc
     4801 tccaagcacc tcaagatgag attatcaaaa aaggcacaaa aggtttagaa aaaccaacct
     4861 tagaatgggt taaaacagat aaagatgtct tgaagaagag tgcgacagcc agttatacat
     4921 tgaataaacc agatggggta gaaatcaaat ccatcaaagt agctctaaaa gataatactg
     4981 gaacagttat taaagaagta acggtagaag aaaataactt aaacgctact ttagacaact
     5041 tgaaatatta tcaaggttat acactatcca caaccatggt ctataatcga ggcgaaggcg
     5101 aagaaactga aacgttagaa gataaagaag ttcagttaga ccttaaaaaa gttgaaatca
     5161 aaaacatcaa agaaactagc ttgatgagtg tggatgatgc tggtgttgaa acagataaga
     5221 gcttacttac tgaaaaaccg acagacgttg ctccacttta cctacgagtg acaactcatg
     5281 ataacaaaac aacaagactg gcagtaagtt cagttgaaga agttgttgtt gatggaaaaa
     5341 cattgtacaa agtggtagct aaagctcctg acttagttca acgtagagaa gatgatacac
     5401 taagcgaaga gtatgtacat tactttgaaa aacaattacc aaaagtagat aatgtgtact
     5461 atagctttaa cgagttaatt acagatatgc aaaaaaatcc tactggtaca ttcaaactag
     5521 gtgcagatct taatgcagcg aatactccaa ctccaagtaa atcatatgta acaggtgaat
     5581 ttaaaggtaa attatcaagt gttgatggtc aacattatac gattcacaat actgcgcgcc
     5641 cattatttaa caatattgtt ggtggtaccg ttaaaaatat taatcttaac aatgtaaata
     5701 tcgatatgcc ttgggctgat caaattgcat ctgtagcaaa tgtgatcaaa ggtggtagcc
     5761 ttattgagaa tgttaaagtc aaaggtaatg tgctcggcag aaactgggta tctggtttca
     5821 ttgataaaat tgacagtggt ggtagattag ttaatgttgc atttattggt aatgtaactt
     5881 ctgttggaga tggggataaa ttcttaacag gtattgttgg agaaaactgg aaaggatatg
     5941 tcgaaaaagc atacgttgaa gctaatatta aaggtaagcg agctaaagct gctggtatat
     6001 cttattggtc gcaaaatagc ggagataata atgctgtcgg tagagatgga gcaattaaga
     6061 aatctgtcgt taaaggttca atagatgtag aaaaaccaat tgaagttggt ggcgctgttg
     6121 gaagtttaaa ttatcttggt tatatagaag acactgttgc tatgatgaaa gttaagaacg
     6181 gtgaaatttt ctacggttcg catgatatcg ataccgatcc atattacaca ggagaacgag
     6241 taaatagaaa tttcattgtt gacggtgtaa gtgaaggtaa atcttcgtat acatactcta
     6301 aacaacaaaa gcgtattaaa tcaatatctc aagaggaagc agataagaaa attaaagaat
     6361 tgggtatcac agcagataaa tttacaatta tcgatccaat tgtaaataaa cttaacaaca
     6421 tcaaacaaaa agctgatact tataaagata ctcaagatta caaagcagac cgtgaactta
     6481 cttatcgtaa tatcgaaaaa cttcaaccat tctacaataa agagtggatt gtagaccaag
     6541 gtaataaagt tccatcaaat tctaaattat taactacaga agtattatct gttacaggta
     6601 taaagagcgg tcaattcgta actgatttgt cagatattga taaaattatg attcactatt
     6661 ctgatggagc aaaggaagaa ttgaatgtta ctcgtcaaga atcaaacgtt caacaagtgc
     6721 gtgagtacag tatcacagat ttagatatac tctatacacc aaatatggtt gagaaagatc
     6781 gcgctcagtt gatgactgat gtaaagtcta agctatctag tgtagaatta gagtctgatg
     6841 gagtgcgtca acttctcgtt aagagagata ctaagaaaga tgcgaatgca aatagtgtag
     6901 gtcgtcaaaa tggatacatt agagatttat tccttgaaga aagtttctct gaagttaaag
     6961 caaatcttga caaattggta aaacagatac ttgaaaatga agatcatcaa ctcaacgata
     7021 acgaattagc tgaacgtgct ttactgaaga aagttgaaga taataaagct aaaatcatga
     7081 tgggactggc atacttaaat caatattatg cttttaaata cgatgagtta tcaattaaag
     7141 atattatgat gtttaaacct gatttttatg gtaagacagc aagtgttatt gatagattga
     7201 ttaatattgg ttctgcagaa aataatctaa aaggagatag aacgcaagat gcttatcgtg
     7261 gtataatttc aggggcaaca ggtaaaggta gtttacatga cttcttgacc tacaatatga
     7321 aattattcac aaatgaaaca gacataaatg tttggtttaa gaaagctatt gaaaagaatg
     7381 cttatgtagt tgaacaacca tcaacgaacc ctgcatttgc caacaaaaaa tatcgtctat
     7441 acgaaggaat taataatggg caacatgggc gcatgatttt acctcttctt aacttgaaaa
     7501 atgcacatct atttatgatt tcaacataca acactatctc gtttagttca tttgaaaaat
     7561 atggcaaaga cacagatgaa aaacgtgaaa agttcaagag tgagatcaat aaacgtgcta
     7621 aagagcaggt taattactta gatttttggt cacgtcttgc tactgataat gtccgtgata
     7681 aacttcttaa gagtcaaaat gtagttccaa ctcctgtatg ggataatcat aatagcccga
     7741 acggatgggc tagtagacat ggtcatatag atgggaaacc ggattatgct ccaattcgtg
     7801 agttctttgg gcgaatcaat aagtatcatg gttacaaata tggatatggc gcatatgcat
     7861 atatttttgc agcacctcaa ccgatggatg cagtttattt cgttatgaca gatttaatta
     7921 gtgactttgg aacatctgca tttacacatg aaactactca cgttaatgat cgtatggctt
     7981 actatggtgg tcattggcat cgtgaaggta cggatcttga agcatttgct caggggatgt
     8041 tacaaacgcc atcggtatca aatccaaacg gagaatatgg cgcattaggt ctaaatatgg
     8101 cgtatgaacg tcaaaatgat ggaaatcaat ggtataacac aaatccaaac gaccttacaa
     8161 ctcgtgcaga aattgataac tacatgaaag gtttcaacga tacattaatg cttcttgatt
     8221 accttgaagg agaagctgtc ctcaataaag caaatcaaga tttaaacaac gcttggttca
     8281 aaaaagtgga taagcaactt cgtggagcta gtacgaagaa ccagtatgat aaagttcgtg
     8341 atttaacggc agatgagaaa aatatcactc taaattcagt agatgattta gttgataata
     8401 attttatgac gaaacacgga cctggaaaca atgtatatga tccaactggg ttcggaacgg
     8461 cctacgtgac tgtacctatt actgcaggta tttatggagg taacacaagt gaaggtgctc
     8521 ctggttctat gtcattcaaa cataacacat tcagaatgtg ggggtactac ggatatgaaa
     8581 aaggattctt aaactatgct tctaacatgc tgaaaaatga gtctagacag gctggtcaca
     8641 atactctagg tgatgatttc atcattaaga aagtttctga taataaattc agtacattgg
     8701 aagattggaa gaaagcatac ttcaaagaag ttgtagataa agctaaagca ggatttaacc
     8761 ctgttacaat cgacggtact acatatagta gttacgatga cttgaagaac gcatttgctg
     8821 cagcagttga aaaagataaa gctactctta aaaatggatc agttaaatct gacaatactg
     8881 ttgcacttaa agagaaaata tacaaaaaac ttcttcaaca aacagatagc tttaaaactt
     8941 caatctttag ataatcgaaa tctctcatct gctttgcgga tgagggtttt cttattttat
     9001 gctatactta aggtatgcat agaaaaacag tgattgattt taggacttta ggggagagat
     9061 atacctttac ccagcctatt aaagagttga aaacgagaaa tgtagaagaa gtggcagatt
     9121 tgctggcaca agtggaaagc taccaagagc aaggttatta tgtggtgggg tatgtcagct
     9181 acgaggctgc acctgctttt gaggagaaat taacagttca caaggttcct ctactgggcg
     9241 agtacttgct ttactttact gttcacgata gggtagagac ctcccctatt cctctgactt
     9301 atgaggatat tgatttgccc tcaaattggc aggaagtgac gtctgcagca gactatgaaa
     9361 aggccattgc ccagatacac catcacttga ggcagggaga cacctatcag gtcaactaca
     9421 ccgtccaact caagcaaaag ttaagtgcca atccttttgc tatctacaat cgtatggtgg
     9481 tagagcagga ggcgggctac aatgcctacg tggaacatga cgagatggca gtgatttcca
     9541 tgagcccaga gctctttttt gagcaaaatg atcgcgagtt gacaacacga ccaatgaagg
     9601 ggacgactca gcgtggggta actgaccaag aagatcttga acaggccagt tggttggaac
     9661 aggatcccaa aaatcgctct gaaaatatga tgattgtgga cctcttgcgc aatgatatga
     9721 accgtatttc tgaagttggg agcgagcacg tggagcgtct gtgtcaggta gagcagtatt
     9781 caactgtttg gcagatgact tcgaccatca agagtcagtt gcgagaggat gtggaccttg
     9841 ttgaaatctt ccgctcactc tttccttgtg gttccataac gggtgcaccg aaaattgcga
     9901 caatggagat tatcaaggac ttggagcctc aaccgcgtgg agtctactgt ggaacgattg
     9961 gtctcttgct tccaaatgga cgacggattt ttaatgtggc cattcgtacc attcaacttc
    10021 acaaagatca agccatttat ggagttggcg gagggattac ttgggatagc acatgggaat
    10081 ctgaataccg agaggttcat caaaaggcag cagttcttta tcgtaaacaa gctcgcttcc
    10141 aactgattac gacagggaaa atcagccaga agaacctgct gtttgaagat caacatctgg
    10201 aaagactgag aaaagccagt cgttattttg cctttccttt tgatgcagaa gacttgggac
    10261 acaagattga ggaagagtgt caggattgtg aagctaatcg agattaccgc ttgcgaatca
    10321 gccttagcaa atctggagag atagaagtca atcgtcaagt attaacccct ctcagtacaa
    10381 gcttttgtca ggcccaagtc tgtcttcagg aagctgcttt gaatcaatcc tttacctact
    10441 ttaaaaccac tcaccgaccg catttgagcc taggagaaca agagaagatt taccacaata
    10501 agtcaggaga actgcttgaa acctctatag gaaatttggt tctgaaaatc gctggaaaac
    10561 tctacacacc gcctatccga cttggaatct tgccaggaat ttaccgtcag catttgctag
    10621 aaacaggaca ggtagaagag aaagtcttga ccttggcaga tttagcccaa gcagaagcta
    10681 tttacggctg taatgcagtg agaggcttgt atgaactaag ccttgaggag aactagatga
    10741 aattgatatt tttgcatggg ttagggcagt cagcagagtc ttggaaagaa gttcggaatc
    10801 tactgacaga ttatccttct gaggctatcg agttatttcc ttctggagtt agtaactatc
    10861 aacaagctaa agagcgagtt tatcagcacc tagctcaaga gacagaacct tttgttttga
    10921 ttggattgtc cttaggtgct gccctgacac tagaactttc cagttacgat ttaccaaatc
    10981 ttcgagcttt gattctgtca ggatgcccgc ttaaattagc agggaatatc ctattttatc
    11041 ttcaattgct gatttttaaa ctacttccca aaagagtatt tgaaaaacag ggagcagaca
    11101 agactcttat ggtcggagtt tctgaggaat tgaagacact tgatttaacg gatatagcag
    11161 ggacttgctc ctatccaacc ttactaattt gtggcagcaa agataaaccg aatcttagtt
    11221 ctatgaaagc tctccataga ctgttaacag attcgcagtt tcagatcatt cctgatggcc
    11281 ctcatgtctt gaataaggca aaaccaaagg agtttgtaga aaagaccaga agtttccttg
    11341 aattgctgaa ataagtttga taaaataata ttgaaatcga tatc
//
LOCUS       DAVAAL010000053        11361 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVAAL010000053 DAVAAL010000000
VERSION     DAVAAL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.054"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..696
                     /locus_tag="VQM60_001184"
     CDS             <1..696
                     /locus_tag="VQM60_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET1081055.1"
                     /translation="HKGNYAYRRIHLELRNRGYLVNHKRVQGLMKVLNLQAKMRQKRK
                     YSSHKGDVGKKAENLIQGQFEGSKTMEQCYTDVTEFAIPASTQKLYLSPVLDGFNSEI
                     IAYNLSTSPNLEQVQTMLEQAFTEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASM
                     SRKGNSQDNGMMESFFGILKSEMFYGYEKTFKSLNQLEQAIVDYIDYYNNKRIKVKLK
                     GLSPVQYRTKSFG"
     gene            complement(824..1027)
                     /locus_tag="VQM60_001185"
     CDS             complement(824..1027)
                     /locus_tag="VQM60_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081056.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(1020..2102)
                     /locus_tag="VQM60_001186"
     CDS             complement(1020..2102)
                     /locus_tag="VQM60_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET1081057.1"
                     /translation="MSKLQQILIYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYYGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(2228..3010)
                     /locus_tag="VQM60_001187"
     CDS             complement(2228..3010)
                     /locus_tag="VQM60_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET1081058.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(3010..4353)
                     /locus_tag="VQM60_001188"
     CDS             complement(3010..4353)
                     /locus_tag="VQM60_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET1081059.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILEAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4452..5768)
                     /locus_tag="VQM60_001189"
     CDS             complement(4452..5768)
                     /locus_tag="VQM60_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET1081060.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5961..7187
                     /locus_tag="VQM60_001190"
     CDS             5961..7187
                     /locus_tag="VQM60_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HET1081061.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            7386..8960
                     /locus_tag="VQM60_001191"
     CDS             7386..8960
                     /locus_tag="VQM60_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET1081062.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LNEKQTGFVIRNKGDK"
     gene            9225..10013
                     /gene="codY"
                     /locus_tag="VQM60_001192"
     CDS             9225..10013
                     /gene="codY"
                     /locus_tag="VQM60_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET1081063.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            10013..10588
                     /locus_tag="VQM60_001193"
     CDS             10013..10588
                     /locus_tag="VQM60_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET1081064.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10670..10755
                     /locus_tag="VQM60_001194"
     tRNA            10670..10755
                     /locus_tag="VQM60_001194"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10704..10706,aa:Leu,seq:aag)
     gene            11039..>11361
                     /locus_tag="VQM60_001195"
     CDS             11039..>11361
                     /locus_tag="VQM60_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081065.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKY"
BASE COUNT         3360 a         2133 c         2468 g         3400 t
ORIGIN      
        1 cacaagggaa attatgctta tcgtcgaatt catttagaac taagaaatcg tggttatctg
       61 gtaaatcata aaagagttca aggcttgatg aaagtactca atttacaagc taaaatgcga
      121 cagaaacgaa aatattcttc tcataaagga gacgttggta agaaagcaga gaatctcatt
      181 caaggccaat ttgaaggctc taaaacaatg gaacagtgct acacagatgt gactgaattt
      241 gccattccag caagtactca aaagctttac ttatcaccag ttttagatgg ctttaatagc
      301 gaaattatcg cctataatct ttcaacttca cccaacttag aacaagtaca aacaatgttg
      361 gaacaggcat tcacagagaa gcactacgag aatacgattc tccatagtga ccaaggctgg
      421 caatatcaac acgattctta tcatcggttc ctagagagta agggaattca agcatctatg
      481 tcacgcaagg gaaacagcca agacaacggt atgatggaat ctttctttgg cattttaaaa
      541 tccgaaatgt tttatggcta tgagaaaaca tttaaatcac ttaaccaatt ggaacaagct
      601 attgtagact atattgatta ctacaacaat aaacgaatta aggtaaaact aaaaggactt
      661 agtcctgtgc aatacagaac taaatccttc ggataaatta tttgtctaac tgtttggggg
      721 cagtacattt taacacttga tactatgcgt tttattgtgg gaagattttc ctcattttct
      781 agtgaaattg aactttttct aacatctttt gatagatgac ttcttaatca attatcattt
      841 ccattgttgt ttcttggggt ttcatccctt gtctttgata aaagcggaga gcaccctcgt
      901 tatcattcca gacacttagg gttagattat tgcagccaat ttctttagca taatagcaga
      961 aaaatgacaa taaaatagcc cctagatatt ttgaaaggaa agcatagggg ctatatagtt
     1021 taatcaaaat aaagcaaatc tttgctggtg cttgtaaaga ggtcgaagcc atccttggta
     1081 acaacaccgc agtcttcaat acgaacaccg actttaccag ggatatagat acctggttca
     1141 acagagaagc acatgccttc ttcgatgacc atgtcgtttc cttccatgat agatgggaat
     1201 tcatggacat ccataccgat accatgcccg agacggtggt tgaagtactc accataacca
     1261 gctttttcga tgacctcacg ggcagcgcgg tccacttcat gagcagtcac acctggcttg
     1321 ataaagtcaa gagcagcttg ttgggcttca agagtcaagt tgtaaatatc tttcttgaat
     1381 tggtctggtt tgccgacagc gactgtacga gtcatatctg acgcatagcc attgaccaga
     1441 acacccaggt caaagaggag aagagcatca ttttcaacct tattagctgc tggaatgccg
     1501 tgtggattcg cagcattatc accagtcaag accatggtat caaagctcat ttcataacct
     1561 tcacgtttca tggcaaagtc gatttgtgcg atgatatctg tctcagtctt atcaagagaa
     1621 atattgtcaa aaccaacatg aacagccttg tcagcataaa gacctgcaac catcattttt
     1681 tgcacttcat cagctgattt gatgaggcgc atgcgttgga tacgaggagt gaggttgtca
     1741 aactcagcag tctcaaaaac tgttttcaaa ccatagtatt tggtcaagat gagattgtca
     1801 aactcaacag cgacacgttt gaagtcaagt tgtggaagag catgtttgat tttttgccat
     1861 ggattttcag aatcgacata gcccactact gggaaggaaa cggtgctact tgcacgttct
     1921 acttcaagag ctgggacaaa gaggagaggt tcctgatccg ctaggacaaa gaggaacatt
     1981 tggcgttcat ggggatcact gtaaaaacca gtgaggtaat tgattgtgac ggggtcagat
     2041 acgacagcga cgtctagttt ttctgattca agatatatta ggatttgttg taatttagac
     2101 atatgctacc ttctttctac ccctctattt tggcaaaaaa tgagagaaaa tgcaagggag
     2161 aagggtaaaa gaaaagacaa aaaactccga caagataacg gagttctttg atatcatttt
     2221 cctttgttta agaaaagtca gccttgcttt tgacgtcact gtactcttca gcgatttctt
     2281 ggctgagaat gtcctcatag gcagggagtt ggatgtcctg accatatttc ttcttgaggg
     2341 cagtagtaac taggcgataa gccagattgg cattacgaga gaggataggc ccgtggaagt
     2401 aggaaccaaa gacattctta taatgaaccc cttcaccgac cttttcttcg ttgtttccat
     2461 ttccatagac aacctgtccc agcggttttt ggtcatcaga gaggaaggta cgaccttggt
     2521 gattttcaaa tccatagtag gtttcatcga aatcttcatt gtgaatcttg atgtcaccga
     2581 taaaacggtt attggtctgg ttgagcgtgt agtgtcccat gacccctagc ccttcgatac
     2641 gttttcctga agcttcaaca taatattgac ccaatagttg gaaaccaccg cagatagcca
     2701 gaactacacc gtcgttttgg atgtagttgt caatgctctc ttttttagca ggtaggtcgt
     2761 ctgcaatgat actttgttca aagtcttgac caccaccgaa aaaggctatg tcgtagtgat
     2821 tttcatcaaa gtcatcatgg agagaaacga tgtcaacggt cacatgggct cccagttttt
     2881 cagccacata cttgagcatg aggatgtttc cattgtcccc gtaggtattc atgagatttc
     2941 cgtagaggtg ggcaatgttg agctgatagg ggtaattgcc atcttttgag gaaagtgaag
     3001 tataaaccat tagttcatct cctttctaac aatctgacga ctagccagca gttcacgaaa
     3061 ttccagcatg gcagtataag ttgccagaat ataggcatgc ttgcagtctt gattctcaat
     3121 ggtcttgaga acttgctcca gattactcgt ttcagtgatt ttctcagctg gatagccagt
     3181 cactcggagg cgacgagcga tttcagaatg acgaacaccg ccagcgttga tttcaggaat
     3241 gtccatgtca gtgatttgct caaagtctgc atcccagatc cagctagtgt caattccatc
     3301 tgcatagttg gcattaagga ggacagatag gctaaatgga taaggtgcta gtttgatcat
     3361 ttcgatagct tgggttgcac cgactggatt tttaatcaag acaagggtac attccttgtc
     3421 accgatatga aaggtttctt ggcgtccaaa gacagcacgg ctcttgtcaa atccctgttt
     3481 gatgagttgc gaatcggcac ctaggaaacg ggcgatggcc acagcagcta gggcgttata
     3541 gatattatag agcccgccga tttggatacc gtattcttgg ccgtctatga caaagcgaga
     3601 gcgattgttg gtcaactcaa ccagttttgt caaacgatag tcgagatcag gacgtttgca
     3661 tccacaacct tcacagatat aggcacccaa gtttgcatag gtattatgct catatttgag
     3721 gatgccttgg cagtcaggac agagaatccc ttcggtattg tagtgagcca gttgggctgg
     3781 tcctttttcc aagtcaaaac caaaatactc tatagggttt ggaatagttg gcttgtagaa
     3841 aagtggactg tctccgttaa ggagaacagt agcagttgga actttccgaa tggcttccaa
     3901 tatcatgtta taggtagtat agatttcacc gaaacggtcc atctggtcac ggaagatatt
     3961 agtaatgaca aaaagactag gctggatata gtcacagata cgagatagac tggcttcgtc
     4021 aatttcgagg acggcaatat ttttcccagt ttttgaagat ttggctgtta ggaaggttgt
     4081 tgcaatccct gtaatcatgt tggcaccgct tgggttggtt agaacttgac cataaacctc
     4141 ttttaaaatg ccgacagtga gggcagttgt cagggttttt ccatttgttc cagtgacaac
     4201 gacaatctcg tagttcttag ctaggttttg taaaatatct ttatcaaatt gaagggcgac
     4261 tttacctggg agcgtacttc cacggccaag acggcttaaa atgaagtggg aagaacgccc
     4321 agcaagaagg cccaaagtag tttttaagtt catgtttcta ttatatcata aaagaggaga
     4381 aagacagatt tgagattccg cagaagtaaa aacagcccaa agagggctgt tcgatacgat
     4441 taaatctcaa cttaaatcgc aaacaagtca ttcaagttct cagccaaggt tgggtgagtg
     4501 aagatttgtt ttgtgaagta agtgtaagga atcttgttgt ccatagcaac agtgatgatg
     4561 ttgatgattt cttgagaacc ttctgagaag atgcttgctc caagaatttc ttttgtttca
     4621 gtattgacaa cagctttgaa ggcaccgcga aggtctccat ttacgtgacc acgaggcatt
     4681 gctgcaacgg ggatttcctt aacagcgtat ggaagtttca aatcagctgc ttggctttca
     4741 gtcaaaccaa cttgtgaaag tgcaggtgtg atgaacatag tatttggcac attgagacgg
     4801 tcttcaagtg tgtagctgcc atctccagca aggtagctgt aaacaacacg gaagtcatca
     4861 agtgaaatgt aagtaaattg aaggccaccg ttgacatctc caactgcaaa gacaccagga
     4921 acgtttgttt gacagtgttt gtctacttta atagcaccac gttcagttag ttcaatatct
     4981 gtattttcaa gttgaagtgg ttctacattt ggtttgcgtc cagttgcgta gagaagggcg
     5041 tcgaaacggt aagtttcgtc ttcagttacg acaagcactt ggtcaccatc gtttttgatt
     5101 tcagtagtat ggatattttg aagcaattca atgccatctt cttccatgta ttgtttagca
     5161 agagctgcga tggaaggttc tgcacgaggt aggaatgtat ccaaggcatc taggactgtg
     5221 accttgcttc caagtttgtt gtaaaggccg gcaaattcaa gaccgatatt tccgccacca
     5281 aggattccaa gtttttcagg taatttgtcc aagctttgga tacctgttga gtcaaagatg
     5341 tttttgcttg tagcaagtcc agggattggc aagacgtttg aaacagcacc agtgttgatg
     5401 acgattgttt cagcagtcag ttctttcttt tcatcaccag cttggatttc gatgacttta
     5461 tttgaaagga agtgagcttc cgcatcaaag atatctacgc ctgtaccagc aacagtcgca
     5521 tagtttttac cgttgaggcg accagtgatc gtgtttttag tagcaatgac ttcttcaaaa
     5581 gacaagtcct tttcagcagc aactagcaag gttttagttg ggatacaacc aatgttgata
     5641 caagttccac cgtacatagc cttgctgcgt tcaacgaggg caactttttt gccagctgaa
     5701 gccaatttac ctgctagtgt tttaccagct ttaccaaatc caataacaat taaatcgtat
     5761 gttaacattt atcttcctcc tattcgaatt tcattctagc acaagaaaaa aacttttgca
     5821 caattctgct acggaaaaga cagaaaaaga taaaaacgct ttccagacaa aaaactagta
     5881 attttttgac aagcttgata aactaaaatc ctatctattt ttatataaga tagaataaat
     5941 gttcgaaaat aggaggtttt atgaaatcga atcgttatat tattgccttt gctggggtca
     6001 ttttacactt aatgctgggt tcgacttatg cttggagtgt ttatcgtaac cctattattg
     6061 aaaaaacggg atgggatcag gcttctgttg ccttcgcctt tagtctagca atcttttgtt
     6121 tgggtttatc ggctgcattt atggggcgtt tggtagaaaa atttggtccg aaagtcatgg
     6181 ggagtctatc tgcttttcta tacgcaggtg gaaatatctt aacaggattt gcaatagacc
     6241 gtcaggaact gtggttgttg tatctagctt atggcatttt aggtgggctt ggtttgggag
     6301 caggctatat tacccctgtg tcaacgatta taaaatggtt tcctgataaa cgtggtctcg
     6361 caacaggttt agcgattatg gggtttggtt ttgcttcttt attgactagt cccatagcgc
     6421 aacaccttat cgcaggggta gggattgtag aaacttttta tattttagga gcaagttact
     6481 ttattatcat gctcctagct tcacaattca ttaagcgtcc aaatgagcaa gagcttgcaa
     6541 ttttatcttc ttcagggaaa gaaaaaacag cctctttgac gcaaggaatg gctgcaaatc
     6601 aggctctaaa aagcaatcgg ttttatatgc tttggattat tttctttatc aacatagctt
     6661 gtggtttagg cttaatttca gcggcatcgc caatggcaca ggagatggct ggcttgtcta
     6721 caagtcatgc agcagtaatg gtgggtgttt tggggatttt caatggattt ggtcgcttgc
     6781 tctgggcgag tttgtctgac tatatcggtc gccctctaac ctttagtata ttactgcttg
     6841 tcaatctttt cttttctctc tcactttggc tctttacaga ttccgtttta tttgtagttg
     6901 ctatgtctat tttgatgact tgctatggag ctggtttttc tttgattcca gcttatctca
     6961 gtgatatttt tggaaccaag gaattggccg ctctgcatgg ttatatttta acagcttggg
     7021 caatggctgg tttagcggga cctattttat tagcagagac ttataaaatg gctcattcgt
     7081 acacacaaac cttgttcgtt tttctcattt tatacagtat cgccttggct ttgtcttatt
     7141 atctaggtcg ttcaatcaaa aaagaaagtc aaaaaccgct tacatgattt tctgtagaaa
     7201 tggggctatc ttttgctctg gtcttgtgtt atactagata ggttgcaaag aaaacagtac
     7261 ttttcttttg tggaaaagaa gcaacacgat tttatatcta gttatatgaa aatacgtcat
     7321 aaaaagaaaa gtataaacta agccctatag ggtggcttcg caccaccttt agaaagaaga
     7381 ataacgtgaa atttaatgaa ttaaacttgt ctgctgattt gctagcagaa attgaaaaag
     7441 ctggttttgt agaagctagt cctatccaag aacaaactat tcctttggcc cttgaaggca
     7501 aggatgttat cggtcaagct cagactggta caggaaaaac tgcagccttt ggcttgccta
     7561 cccttgaaaa aatccgtaca gaagaagcga ctatccaagc tttggtcatc gctccaactc
     7621 gtgaactagc tgtccaaagt caagaagaac tcttccgctt tggtcgtagt aagggagtca
     7681 aagtccgttc agtatatggc ggatcaagca ttgaaaaaca aattaaggct cttaaatctg
     7741 gtgcccatat cgtggtggga actccaggtc gcctcttgga cttgattaaa cgcaaggcct
     7801 tgaaattaca agacattgaa acccttatcc ttgacgaagc ggatgaaatg cttaacatgg
     7861 gcttccttga agacatcgaa gccattattt cccgtgtacc tgagaaccgt caaactttgc
     7921 ttttctcagc aactatgcca gatgccatca aacgtatcgg tgttcagttt atgaaagccc
     7981 ctgaacatgt caagattgcg gctaaggaat tgacaacaga attggttgac cagtactata
     8041 tccgtgttaa ggaacaagaa aaatttgaca ccatgactcg tctcatggat gtggcacaac
     8101 cagaactcgc tattgtattt ggtcgtacca aacgccgtgt ggatgaattg actcgtggtt
     8161 tgaaaattcg tggcttccgt gcagaaggaa ttcatggcga cctagaccaa aacaaacgtc
     8221 ttcgtgtcct tcgtgacttt aaaaatggca atcttgatgt tttggttgcg acagacgttg
     8281 cagcgcgtgg tttggatatt tcaggtgtga cccatgtcta caactacgat attccacaag
     8341 atcctgagag ttatgttcac cgtatcggtc gtacaggtcg tgctggtaag tcaggtcaat
     8401 ctattacttt tgttgctcca aacgaaatgg gttaccttca aatcattgaa aacttgacta
     8461 agaaacgcat gaaaggtctc aaacctgcaa gtgtagaaga atccttccaa tcaaaaaaac
     8521 aggtagctct caagaaaatc gaacgtgatt ttgcagatga aaccattcgt gccaactttg
     8581 agaaatttgg taaggatgct cgcaaattgg ctgctgagtt tactccagaa gaattggcaa
     8641 tgtatatctt gagtctgaca gtccaagacc cagatagcct tccagaagtg gagattgcac
     8701 gtgaaaaacc actaccgttt aaaccatcag gtaatggttt cggtggtaaa gctaagggag
     8761 gtcgtggagg ccgtcgtggg gatgaccgtc gagagcgtga tcgccgtggc aatggtcgcc
     8821 gtgatgagtt caaaaaagga agtcgtggca acgatcgttt tgataaggaa aaacgttacc
     8881 gtaaggataa taaaaaacca cgcaatactt taaacgaaaa gcaaacaggc tttgttattc
     8941 gtaacaaagg tgataaataa gaaaaagcgg ttcaaaatga atcgcttttt tatataagga
     9001 gaccgaatgt gaaaaagaca aataaaatta ttatattatc ttttataata ttatgataca
     9061 aaaaaatagg caacttgtca atagaaaagg aacaatttaa ttaaaaatat tctggttttt
     9121 aattaaattg taagtcaatg caagtcaata aggaattttc agataattat tgaaaaagag
     9181 acgtttttat gttataatga tagcgattaa attcgaggtg aaaaatggca catttattag
     9241 aaaaaactag aaaaattaca tcaattttga aacgctcaga ggagcagttg caggatgagc
     9301 ttccttacaa cgctattacg cgtcagttag cggatattat tcattgcaat gcctgcatta
     9361 tcaatagtaa gggacgtctg cttggctatt ttatgcgtta taaaacaaat acagatcgcg
     9421 tagagcaatt cttccaaact aagattttcc cagatgacta cgttcaaggg gctaatatga
     9481 tttacgaaac agaagcaaac ttacctgttg agcatgatat gagtattttc cctgttgaga
     9541 gtagagatga ttttccagat ggcttgacga ctattgcacc gattcatgta tcggggattc
     9601 gccttggttc tttgattatt tggcgtaatg ataaaaaatt cgaagatgag gacttggttc
     9661 ttgttgagat tgccagtacc gttgttggga ttcagcttct taacttccaa cgtgaagaag
     9721 atgagaaaaa tattcgtcgt cgtactgctg tcaccatggc ggtcaatacc ctttcttact
     9781 ccgaactccg tgctgtttca gcaattttag gggaattaaa tggaaatgaa gggaagttga
     9841 ctgcgtcagt gattgcagat cgtatcggaa tcactcgctc tgtgattgtc aatgctcttc
     9901 gtaaacttga gtctgcgggg attattgaaa gtcgctcact tggaatgaag ggaacctatc
     9961 ttaaggtctt gatttcagat atttttgaag aagtgaagaa aagagattac taatgacaaa
    10021 ggctttaatt tcgattgatt atacagaaga ttttgttgct gatagtggga aactaacagc
    10081 aggtgctcca gctcaggcga tttcggatgc catcagcaag gtaactcgat tagcttttga
    10141 acgaggagat tatatcttct ttactattga tgctcatgaa gaaaacgatt gtttccatcc
    10201 ggaaagtaag ttatttcctc ctcataatct gattgggacg agtggacgga atttatatgg
    10261 agatttgggg atcttttatc aagagcatgg ttcagacagt cgtgtctttt ggatggataa
    10321 acgccattac tcagcttttt cagggactga cctagatatt cgtttgagag agcgtagagt
    10381 gtctaccgtt atcttaacag gtgtcttgac ggatatctgt gtcctacata cagctataga
    10441 tgcctataat ctaggatatg acatcgagat tgttaaacca gctgttgctt ccatctggcc
    10501 tgaaaatcat caatttgccc taggtcattt caaaaataca ctcggagcta agttagtaga
    10561 tgaaaatcta aatgaacttt ctgagtaacc tggttggtga aaatgaaaaa atttgaaaaa
    10621 aagtgttgac aagcatccca aaagttgata tactagtaaa gtaatcgacg cggggatggc
    10681 ggaattggca gacgcgcagg actaaggatc ctgtgaccgc tttaggtcgt gagggttcaa
    10741 gtccctctct ccgcatagga aaagagctag ctaaagctag ctcttttgta tttctctaaa
    10801 aaattagaat ccagtcaaat gtagtggaga atcagcgaaa atatgggaaa actgatcctt
    10861 gtttctgctt ttgtctatag aagaataata aagattatct tcttcaaatt ctccgatatt
    10921 ctctaaagtt ttgtgcaagt tgcacagaac ttttttattt ttttggtcat cttgccatag
    10981 aaatataaag cgttttcatc tataatataa ttatcaaaag acaaaaggag ttcacctcat
    11041 ggtagaattg aatcttaaaa atatttacaa aaaatatcca aacagcgaac actattcagt
    11101 tgaagatttc aacttgaaca tcaaagataa agaatttatc gttttcgtag gaccttcagg
    11161 atgtggtaaa tcaactacac tccgtatgat tgctggtctt gaagacatta cagaaggtac
    11221 tgcatctatc gatggcgtgg ttgtcaacga cgtagctcca aaagaccgtg atatcgccat
    11281 ggtattccaa aactacgctc tttacccaca catgactgtt tatgacaaca tggctttcgg
    11341 tttgaaattg cgtaaataca g
//
LOCUS       DAVAAL010000054        11166 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVAAL010000054 DAVAAL010000000
VERSION     DAVAAL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.055"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            39..1154
                     /gene="ychF"
                     /locus_tag="VQM60_001196"
     CDS             39..1154
                     /gene="ychF"
                     /locus_tag="VQM60_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET1081066.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            1225..1794
                     /locus_tag="VQM60_001197"
     CDS             1225..1794
                     /locus_tag="VQM60_001197"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET1081067.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDEYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            1795..5304
                     /gene="mfd"
                     /locus_tag="VQM60_001198"
     CDS             1795..5304
                     /gene="mfd"
                     /locus_tag="VQM60_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET1081068.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLMSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKELMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            5362..5628
                     /locus_tag="VQM60_001199"
     CDS             5362..5628
                     /locus_tag="VQM60_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET1081069.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            5621..5989
                     /locus_tag="VQM60_001200"
     CDS             5621..5989
                     /locus_tag="VQM60_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET1081070.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            6109..7377
                     /locus_tag="VQM60_001201"
     CDS             6109..7377
                     /locus_tag="VQM60_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET1081071.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGELTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            7374..8651
                     /gene="tilS"
                     /locus_tag="VQM60_001202"
     CDS             7374..8651
                     /gene="tilS"
                     /locus_tag="VQM60_001202"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET1081072.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            8655..9197
                     /gene="hpt"
                     /locus_tag="VQM60_001203"
     CDS             8655..9197
                     /gene="hpt"
                     /locus_tag="VQM60_001203"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET1081073.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            9213..>11166
                     /gene="ftsH"
                     /locus_tag="VQM60_001204"
     CDS             9213..>11166
                     /gene="ftsH"
                     /locus_tag="VQM60_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET1081074.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         3846 a         1650 c         2312 g         3358 t
ORIGIN      
        1 aatattaggg attgaaatga aaacggagaa tgagaaatat ggctttgaca gcaggtatcg
       61 ttggtttgcc aaacgttggt aaatcaacac tatttaatgc aattacaaaa gcaggagcag
      121 aggcagcaaa ctacccattt gcgactattg atccaaatgt tggaatggtg gaagttccag
      181 atgaacgcct acaaaaacta actgaaatga taactcctaa aaagacagtt cccacaacat
      241 ttgaatttac ggatattgca gggattgtaa aaggagcttc aaaaggagaa gggctaggga
      301 ataaattctt ggccaatatt cgtgaagtag atgcgattgt tcacgtagtt cgtgcttttg
      361 atgatgaaaa tgtgatgcgc gagcaaggac gtgaagacgc ctttgtagat ccacttgcag
      421 atattgatac aattaatctg gaattaattc ttgctgactt agaatcagtg aacaaacgat
      481 atgcgcgtgt agaaaagatg gcacgtacgc aaaaagataa agaatcagta gcagaattca
      541 atgttcttca aaagattaaa ccagtcctag aagacgggaa atcagctcgt accattgaat
      601 ttacagatga ggaacaaaag gttgtcaaag gtcttttcct tttgacgact aaaccagttc
      661 tttatgtagc taatgtggac gaggatgtgg tttcagaacc tgactctatc gactatgtca
      721 aacaaattcg tgaatttgca gcgacagaaa atgctgaagt agtcgttatt tctgcgcgtg
      781 ctgaggaaga aatttctgaa ttggatgatg aagataaaaa agagtttctt gaagccattg
      841 gtttgacaga atcaggtgta gataagttga cgcgtgcagc ttaccacttg cttggattgg
      901 gaacttactt cacagctggt gaaaaagaag ttcgcgcttg gactttcaaa cgtggtatga
      961 aggctcctca agcagctggt attatccact cagactttga aaaaggcttt attcgtgcag
     1021 taaccatgtc atatgaagat ctagtgaaat acggatctga aaaggccgta aaagaagctg
     1081 gacgcttgcg tgaagaagga aaagaatata tcgttcaaga tggcgatatc atggaattcc
     1141 gctttaatgt ctaaaaatta ataaatggtg tcaattaggt tggaaaaaaa ttccaaccct
     1201 tttggctttt gaaaggaaaa ataaatgacc aaattacttg taggcttggg aaatccaggg
     1261 gataaatatt ttgaaacaaa acacaatgtt ggttttatgt tgattgatca actagcgaag
     1321 aaacagaatg tcacttttac acacgataag atatttcaag ctgacctagc atcctttttc
     1381 ctaaatggag aaaaaattta tctggttaaa ccaacgacct ttatgaatga aagtggaaaa
     1441 gcagttcatg ctttattaac ttactatggt ttggatattg acgatttact tatcatttac
     1501 gatgatcttg acatggaagt tgggaaaatt cgtttaagag caaaaggctc agcaggtggt
     1561 cataatggta tcaagtctat tattcaacat ataggaactc aggtctttaa ccgtgttaag
     1621 attggaattg gaagacctaa aaatggtatg tcagttgttc atcatgtttt gagtaagttt
     1681 gacagggatg agtatatcgg tattttacag tctgttgaca aagttgacga ttctgtaaac
     1741 tactatttac aagagaaaaa ttttgagaaa acaatgcaga ggtataacgg ataaatggtg
     1801 accttattag atttattctc agaaaatgat cagattaaaa aatggcatca aaatttaaca
     1861 gataagaaaa gacaactaat acttggttta tcaacatcta ctaaggctct tgcaattgca
     1921 agcagtttag aaaaagaaga taggattgtg ttattgatgt caacttatgg agaagcagaa
     1981 ggacttgtta gtgatcttat atctatcttg ggtgaggaac tcgtctatcc atttttggta
     2041 gatgatgctc ctatggtgga gtttttgatg tcttcacagg aaaaaattat ttcacgggtt
     2101 gaagccttgc gttttttgac tgattcatct aagaaaggga ttttagtttg taatatcgca
     2161 gcaagtcgat tgattttacc gtctcccaat gcattcaaag atagtattgt aaaaatctca
     2221 gttggtgaag aatatgatca acacgcgttt atccatcagt taaaggaaaa tggctatcga
     2281 aaagttactc aagtacaaac tcagggcgaa tttagtcttc gaggagatat tttagatatt
     2341 tttgaaatat cccagttaga accttgtcga attgagtttt ttggtgatga aattgatggt
     2401 atcaggtcat ttgaagtaga aacacaatta tcgaaagaaa ataagacaga actcactatc
     2461 tttccagcta gtgatatgct tttgagagaa aaggattatc aacgaggaca gtcagcttta
     2521 gaaaaacaaa tttcaaaaac tttatcacct attttgaaat catacctaga agaaattctt
     2581 tcaagttttc accaaaaaca aagtcatgca gactctcgga agtttttatc tttgtgctat
     2641 gataagacat ggactgtctt tgattatatt gaaaaagata ctccaatatt ctttgatgat
     2701 tatcaaaaat tgatgaatca gtatgaagtc tttgaaagag aattagcgca gtactttaca
     2761 gaagaattac agaatagtaa agcattttct gatatgcagt atttttctga tattgaacaa
     2821 atctataaaa aacaaagtcc agtgaccttt ttttctaatc ttcaaaaggg tttaggaaat
     2881 ctcaaatttg acaaaattta tcaattcaat caatatccta tgcaggaatt tttcaatcag
     2941 ttttcttttc taaaagaaga aattgaacga tataaaaaaa tggattacac cattattctg
     3001 cagtctagca attcaatggg aagtaaaaca ttggaggata tgttagagga atatcagatt
     3061 aaattggatt ctagagataa gacaaatatc tgtaaagaat ctgtaaactt aatagagggt
     3121 aatctcagac atggttttca ttttgtagat gaaaagattt tattgataac tgaacatgag
     3181 atttttcaaa agaaattaaa gcgtcgtttt cgaagacaac atgtttcaaa tgcagagaga
     3241 ttaaaagatt acaatgaact tgaaaaaggg gactatgttg tccatcatat ccatgggatt
     3301 ggtcaatatc taggaattga aaccattgaa atcaagggaa ttcatcgtga ttatgtcagt
     3361 gtccaatacc aaaatggtga tcaaatttct attcccgtgg aacagattca tctactgtcc
     3421 aaatatattt caagtgatgg taaagctcca aaactcaata aattaaatga cggtcatttt
     3481 aaaaaggcca agcaaaaggt taagaaccag gtagaggata tagctgatga tttaatcaaa
     3541 ctctactctg aacgtagtca gttgaagggt tttgctttct cagctgatga tgatgatcaa
     3601 gatgcctttg atgatgcttt cccttatgtt gaaacggatg atcaacttcg tagtattgag
     3661 gaaatcaaga gggatatgca ggcttctcag ccaatggatc gacttttagt tggggatgtt
     3721 ggttttggaa agactgaagt tgctatgcgt gcagccttta aagcagtcaa tgatcacaaa
     3781 caggttgtca ttctagttcc gacgacggtt ttagcgcaac agcactatac gaattttaag
     3841 gaacgattcc aaaattttgc agttaatatt gatgtgttga gtcgctttag aagtaaaaaa
     3901 gagcagactg caacacttga aaaattgaaa aacggtcaag tcgatatttt gattggaaca
     3961 catcgtgttt tgtcaaaaga tgttgtgttt gctgatttgg gcttgatgat tattgatgag
     4021 gaacagcgat ttggtgtcaa gcataaggaa actttgaaag aactgaagaa acaagtggat
     4081 gtcctaacct tgaccgctac gccaatccct cgtaccctcc atatgtctat gctgggaatc
     4141 agagatttat ctgttattga aactccgccg actaatcgct atcctgttca gacctatgtt
     4201 ttggaaaaga atgatagtgt cattcgtgat gctgtcttgc gtgaaatgga gcgtggaggt
     4261 caagtttatt atctttacaa caaagttgac acaattgttc agaaggtttc agaattacag
     4321 gagttgattc cggaggcttc gattggatat gttcatggtc gaatgagtga agtccagttg
     4381 gaaaatactc tattagactt tattgaggga caatacgata tcttggtgac gactactatt
     4441 attgagacag gggtggacat tccaaatgct aatactttat ttattgaaaa tgcggaccat
     4501 atgggcttgt caaccttata tcagttaaga ggaagagtcg gtcgtagtaa tcgtattgct
     4561 tatgcttatc tcatgtatcg tccagaaaaa tcaatcagtg aagtctctga aaagagatta
     4621 gaagcgatta aaggatttac agaattgggc tctggcttta agattgcaat gcgagatctt
     4681 tcgattcgtg gagcaggaaa tcttttagga aaatcccagt ctggtttcat tgattctgtt
     4741 ggttttgaat tgtattcgca gttattagag gaagctattg ctaaacgaaa cggtaatgct
     4801 aacgctaaca caagaaccaa agggaatgct gagttgattt tgcaaattga tgcctatctt
     4861 cctgatactt atatttctga tcaacgacat aagattgaaa tttacaagaa aattcgtcaa
     4921 attgacaacc gtgtcaatta tgaagagtta caagaggagt tgatagaccg ttttggagaa
     4981 tacccagatg tagtagccta tcttttagag attggtttgg tcaaatcata cttggacaag
     5041 gtctttgttc aacgtgtgga aagaaaagat aataaaatta caattcaatt tgaaaaagtc
     5101 actcaacgac tgtttttagc tcaagattat tttaaagctt tatccgtaac gaacttaaaa
     5161 gcaggcatcg ctgagaataa ggaattaatg gagcttgtat ttgatgttca aaataagaaa
     5221 gattatgaaa ttttagaagg tctgctgatt tttggagaaa gtttattaga gataaaagag
     5281 tctaaggaag aaaattccat ttgatatttt tcttctataa aatagataaa aatggtacaa
     5341 taataaattg aggtaataag gatgagatta gataaatatt taaaagtatc gcgaattatc
     5401 aagcgtcgta cagtcgcaaa ggaagtagca gataaaggta gaatcaaggt taatggaatc
     5461 ttggccaaaa gttcaacgga cttgaaagtt aatgaccaag ttgaaattcg ctttggcaat
     5521 aagttgctgc ttgtaaaagt actagagatg aaagatagta caaaaaaaga agatgcagca
     5581 ggaatgtatg aaattatcag tgaaacacgg gtagaagaaa atgtctaaaa atattgtaca
     5641 attgaataat tcttttattc aaaatgaata ccaacgtcgt cgctacctga tgaaagaacg
     5701 acaaaaacgg aatcgtttta tgggaggggt attgattttg attatgctat tatttatctt
     5761 gccaactttt aatttagcgc agagttatca gcaattactc caaagacgtc agcaattagc
     5821 agacttgcaa actcagtatc aaactttgag tgatgaaaag gataaggaga cagcatttgc
     5881 taccaagttg aaagatgaag attatgctgc taaatataca cgagcgaagt actattattc
     5941 taagtcgagg gaaaaagttt atacgattcc tgacttgctt caaaggtgat aaaatggaaa
     6001 atttattaga cgtaatagag caatttttgg gtttgtcaga tgaaaagttg gaagaattgg
     6061 ctgataaaaa tcaattattg cgtttacaag aagaaaagga aaggaagaat gcgtaaattc
     6121 ttaattattt tgttgctacc aagttttttg accatttcaa aagtcgttag cacagaaaaa
     6181 gaagtcgtct atacttcgaa agaaatttat tacctttcac aatctgactt tggtatttat
     6241 tttagagaaa aattaagttc tcccatggtt tatggagagg ttcctgttta tgcgaatgaa
     6301 gatttagtag tggaatctgg ggaattgact cccaaaacaa gttttcaaat aaccgagtgg
     6361 cgcttaaata aacaagggat tccagtattt aagctatcaa atcatcaatt tatagctgcg
     6421 gacaaacgat ttttatatga tcaatcagag gtaactccaa caataaaaaa agtatggtta
     6481 gaatctgact ttaaactgta caatagtcct tatgatttaa aagaagtgaa atcatcctta
     6541 tcagcttatt cgcaagtatc aatcgacaag accatgtttg tagaaggaag agaatttcta
     6601 catattgatc aggctggatg ggtagctaaa gaatcaactt ctgaagaaga taatcggatg
     6661 agtaaagttc aagaaatgtt atctgaaaaa tatcagaaag attctttctc tatttatgtt
     6721 aagcaactga ctactggaaa agaagctggt atcaatcaag atgaaaagat gtatgcagcc
     6781 agcgttttga aactctccta tctctattat acgcaagaaa aaataaatga gggtctttat
     6841 cagttagata cgactgtaaa atacgtatct gcagtcaatg attttccagg ttcttataaa
     6901 ccagagggaa gtggtagtct tcctaaaaaa gaagataata aagaatattc tttaaaggat
     6961 ttaattacga aagtatcaaa agaatctgat aatgtagctc ataatctatt gggatattac
     7021 atttcaaacc aatctgatgc cacattcaaa tccaaaatgt ctgccattat gggagatgat
     7081 tgggatccaa aagaaaaatt gatttcttct aagatggccg ggaagtttat ggaagctatt
     7141 tataatcaaa atggatttgt gctagagtct ttgactaaaa cagattttga tagtcagcga
     7201 attgccaaag gtgtttctgt taaagtagct cataaaattg gagatgcgga tgaatttaag
     7261 catgatacgg gtgttgtcta tgcagattct ccatttattc tttctatttt cactaagaat
     7321 tctgattatg atacgatttc taagatagcc aaggatgttt atgaggttct aaaatgaggg
     7381 aaccagattt tttaaatcat tttctcaaga agggatattt caaaaagcat gctaaggcgg
     7441 ttctagctct ttctggtgga ttagattcta tgtttctatt taaggtattg tctacttatc
     7501 aaaaagagtt agagattgaa ttgattctgg ctcatgtgaa tcataagcag agaattgaat
     7561 cagattggga agaaaaggaa ttaaggaagt tggctgctga agcagagctt cctatttata
     7621 tcagcaattt ttcaggagaa ttttcagaag cgcgtgcacg aaattttcgt tatgattttt
     7681 ttcaagaggt tatgaaaaag acaggtgcga cagctttagt cactgcccac catgctgatg
     7741 atcaggtgga aacgattttg atgcgcttga ttcgaggaac tcgcttgcgc tatctatcag
     7801 gaattaagga gaagcaagta gtcggagaga tagaaatcat tcgtcccttc ttgcattttc
     7861 agaaaaaaga ctttccatca atttttcact ttgaagatac atcaaatcag gagaatcatt
     7921 attttcgaaa tcgtattcga aactcttact taccagaatt ggaaaaagaa aatcctcgat
     7981 ttagggatgc aatcttaggc attggcaatg aaattttaga ttatgatttg gcaatagctg
     8041 aattatctaa caatattaat gtggaagatt tacagcagtt attttcttac tctgagtcta
     8101 cacaaagagt tttacttcaa acttatctga atcgttttcc agatttgaat cttacaaaag
     8161 ctcagtttgc tgaagttcag cagattttaa aatctaaaag ccagtatcgt catccgatta
     8221 aaaatggcta tgaattgata aaagagtacc aacagtttca gatttgtaaa atcagtccgc
     8281 aggctgatga aaaggaagat gaacttgtgt tacactatca aaatcaggta gcttatcaag
     8341 gatatttatt ttcctttgga cttccattag aaggtgaatc aattcaacaa atacctgttt
     8401 cacgtgaaac atccatacac attcgtcatc gaaaaacagg agatgttttg attcaaaatg
     8461 ggcatagaaa aaaactcaga cgtttattta ttgatttgaa aatccctatg gaaaagagaa
     8521 actctgctct tattattgag caatttggtg aaattgtctc aattttggga attgcgacca
     8581 ataatttgag taaaaaaacg aaaaatgata taatgaacac tgtactttat atagaaaaaa
     8641 tagataggta aaaaatgtta gaaaacgata ttaaaaaagt cctcgtttca cacgatgaaa
     8701 ttacagaagc agctaaaaaa ctaggtgctc aattaactaa agattatgca ggaaaaaatc
     8761 caatcttagt tgggatttta aaaggatcta ttccttttat ggctgaattg gtcaaacata
     8821 ttgatacaca tattgaaatg gacttcatga tggtttctag ctaccatggt ggaacagcaa
     8881 gtagtggtgt tatcaatatt aaacaagatg tgactcaaga tatcaaagga agacatgttc
     8941 tatttgtaga agatatcatt gatacaggtc aaactttgaa gaatttgcga gatatgttta
     9001 aagcaagaga agcagcttct gttaaaattg caaccttgtt ggataaacca gaaggacgtg
     9061 ttgtagaaat tgaggcagac tatacctgct ttactatccc aaatgagttt gtagtaggtt
     9121 atggtttaga ctacaaagaa aattatcgta atcttcctta tattggagta ttgaaagagg
     9181 aagtgtattc aaattagaaa gaataatctt taatgaaaaa acaaaataat ggtttaatta
     9241 aaaatccttt tctatggtta ttatttatct ttttccttgt gacaggattc cagtatttct
     9301 attctgggaa taactcagga ggaagtcagc aaatcaacta tactgagttg gtacaagaaa
     9361 ttaccgatgg taatgtaaaa gaattaactt accaaccaaa tggtagtgtt atcgaagttt
     9421 ctggtgtcta taaaaatcct aaaacaagta aagaaggaac aggtattcag tttttcacgc
     9481 catctgttac taaggtagag aaatttacca gcactattct tcctgcagat actaccgtat
     9541 cagaattgca aaaacttgct actgaccata aagcagaagt aactgttaag catgaaagtt
     9601 caagtggtat atggattaat ctactcgtat ccattgtgcc atttggaatt ctattcttct
     9661 tcctattctc tatgatggga aatatgggag gaggcaatgg ccgtaatcca atgagttttg
     9721 gacgtagtaa ggctaaagca gcaaataaag aagatattaa agtaagattt tcagatgttg
     9781 ctggagctga ggaagaaaaa caagaactag ttgaagttgt tgagttctta aaagatccaa
     9841 aacgattcac aaaacttgga gcccgtattc cagcaggtgt tcttttggag ggacctccgg
     9901 ggacaggtaa gactttgctt gctaaggcag tcgctggaga agcaggtgtt ccattcttta
     9961 gtatctcagg ttctgacttt gtagaaatgt ttgtcggagt tggagctagt cgtgttcgct
    10021 ctctttttga ggatgccaaa aaagcagcac cagctatcat ctttatcgat gaaattgatg
    10081 ctgttggacg tcaacgtgga gtcggtctcg gcggaggtaa tgacgaacgt gaacaaacct
    10141 tgaaccaact tttgattgag atggatggtt ttgagggaaa tgaagggatt atcgtcatcg
    10201 ctgcgacaaa ccgttcagat gtacttgatc ctgccctttt gcgtccagga cgttttgata
    10261 gaaaagtatt ggttggccgt cctgatgtta aaggtcgtga agcaatcttg aaagttcacg
    10321 ctaagaacaa gcctttagca gaagatgttg atttgaaatt agtggctcaa caaactccag
    10381 gctttgttgg tgctgattta gagaatgtct tgaatgaagc agctttagtt gctgctcgtc
    10441 gcaataaatc gataattgat gcttcagata ttgatgaagc agaagataga gttattgctg
    10501 gaccttctaa gaaagataag acagtttcac aaaaagaacg agaattggtt gcttaccatg
    10561 aggcaggaca taccattgtt ggtctagtct tgtcgaatgc tcgcgttgtc cataaggtta
    10621 caattgtacc acgcggccgt gcaggcggat acatgattgc acttcctaaa gaggatcaaa
    10681 tgcttctatc taaagaagat atgaaagagc aattggctgg cttaatgggt ggacgtgtag
    10741 ctgaagaaat tatctttaat gtccaaacta caggagcttc aaacgacttt gaacaagcga
    10801 cacaaatggc acgtgcaatg gttacagagt acggtatgag tgaaaaactt ggcccagtac
    10861 aatatgaagg aaaccatgct atgcttggtg cacagagtcc tcaaaaatca atttcagaac
    10921 aaacagctta tgaaattgat gaagaggttc gttcattatt aaatgaggca cgaaataaag
    10981 ctgctgaaat tattcagtca aatcgtgaaa ctcacaagtt aattgcagaa gcattattga
    11041 aatacgaaac attggatagt acacaaatta aagctcttta cgaaacagga aagatgcctg
    11101 aagcagtaga agaggaatct catgcactat cctatgatga agtaaagtca aaaatgaatg
    11161 acgaaa
//
LOCUS       DAVAAL010000055        11058 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVAAL010000055 DAVAAL010000000
VERSION     DAVAAL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11058
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.056"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            239..1600
                     /gene="trpE"
                     /locus_tag="VQM60_001205"
     CDS             239..1600
                     /gene="trpE"
                     /locus_tag="VQM60_001205"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET1081075.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPIFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAVGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            1597..2163
                     /locus_tag="VQM60_001206"
     CDS             1597..2163
                     /locus_tag="VQM60_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET1081076.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            2174..3178
                     /gene="trpD"
                     /locus_tag="VQM60_001207"
     CDS             2174..3178
                     /gene="trpD"
                     /locus_tag="VQM60_001207"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET1081077.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            3175..3942
                     /gene="trpC"
                     /locus_tag="VQM60_001208"
     CDS             3175..3942
                     /gene="trpC"
                     /locus_tag="VQM60_001208"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET1081078.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            3929..4528
                     /locus_tag="VQM60_001209"
     CDS             3929..4528
                     /locus_tag="VQM60_001209"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET1081079.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTELAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            4506..5729
                     /gene="trpB"
                     /locus_tag="VQM60_001210"
     CDS             4506..5729
                     /gene="trpB"
                     /locus_tag="VQM60_001210"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET1081080.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            5722..6498
                     /locus_tag="VQM60_001211"
     CDS             5722..6498
                     /locus_tag="VQM60_001211"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET1081081.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(6470..6571)
                     /locus_tag="VQM60_001212"
                     /pseudo
     CDS             complement(6470..6571)
                     /locus_tag="VQM60_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            6800..7531
                     /locus_tag="VQM60_001213"
     CDS             6800..7531
                     /locus_tag="VQM60_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081082.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            7546..8697
                     /locus_tag="VQM60_001214"
     CDS             7546..8697
                     /locus_tag="VQM60_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET1081083.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFIIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(8749..9408)
                     /locus_tag="VQM60_001215"
     CDS             complement(8749..9408)
                     /locus_tag="VQM60_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET1081084.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFILQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            9478..9924
                     /locus_tag="VQM60_001216"
     CDS             9478..9924
                     /locus_tag="VQM60_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081085.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFPLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
     gene            10069..10341
                     /locus_tag="VQM60_001217"
                     /pseudo
     CDS             10069..10341
                     /locus_tag="VQM60_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10451..10654)
                     /locus_tag="VQM60_001218"
     CDS             complement(10451..10654)
                     /locus_tag="VQM60_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET1081086.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(10685..>11058)
                     /locus_tag="VQM60_001219"
     CDS             complement(10685..>11058)
                     /locus_tag="VQM60_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET1081087.1"
                     /translation="TQVAVDLNVIVEYQKNVPALYSEIREIVSSEVAKMTDLEIVEIN
                     VNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQFEKAKSGLGSGFSTVQEKVSE
                     GVEAVKGAANGVVSHENTRVN"
BASE COUNT         3437 a         2030 c         2511 g         3080 t
ORIGIN      
        1 aagcagagaa agctgtaaag ttgggttcat ctgaaacgag agaagaaaac atcattctca
       61 agggagtgcc ctttatcgca cagcctgtta caggagatat ctgagacagg aatgagagat
      121 aggagaaatc ctataattga ggtggcaccg cgaatttcgt cctcacgcaa gttattttgc
      181 gtggggattt ttcatacagt cgctacgaac tagaattaga actgaaagga aaattacaat
      241 ggaacgaatc attcatggag atgtcttatc accaatcttg gcttatatgc gcttaaaggg
      301 gcaacacaag gttatcttag agagtattcc gagagacaag gaaacagctc gtttttctat
      361 cctagcctat aatccaattt ttgagattaa gtttgaaaat ggagttctct atcaaaatgg
      421 tcaagtgatt gatcgggatc ccttggattt cctttatgaa gtgattcata agagtcagca
      481 ccactcagag ctaccttttg gtggtggagc cgttggtttt gtgggttacg atatgatttc
      541 actttatgaa gaaattggtc aaatccctga ggatacaatt gggacgccag acatgcattt
      601 ctttgtctat gagagctata tggtctttga ccacaagaag gagaagatac atgtcataga
      661 ggatgcactt tatagtgagc gcagtcaaga ggccttggaa aaatccttga accaagtgct
      721 tgaggaatta cgcattcctg ctccaaatga atttgaagac ttggatctat ctccgttaga
      781 cttcaaacct catatcgctc ctcagaagtt tgaggaaatg gtggaaacag ctcgtgactt
      841 gattcgtaat ggtgatatgt tccaatgcgt actcagccag cgcttttcag cagaggttac
      901 tggaaatcca tttgacttct acagaaatct ccgcgtgacc aatccatcta attaccttta
      961 tttctatgac tttggggatt atcaaatcat cggtgccagt ccagaaagtt tggtttctgt
     1021 caaaaatggc atcgtgacaa ccaatccgat tgcagggacg cgaccaagag gggctacgga
     1081 tgaagaagac aagaccttgg cgactgacct cctctctgat gagaaggaaa cagcagaaca
     1141 tcgaatgttg gtagacttgg ggcgtaacga tattggccgc atctctgaaa cggccagtgt
     1201 ccaagttact aagtatatgg aggtggagct cttccgctac gtcatgcatt tgaccagtgt
     1261 ggtcaaggga cgtttgcttg cagaactcac tgccatggat gccttgaaag caacgcttcc
     1321 tgctggaacc gtttctggag caccaaagat tcgggcgatg agacgcatct atgaactgga
     1381 aacggaaaaa cggggcgtat atgcaggagc aatcggctac ttgtctgcga cgggtgatat
     1441 ggatttggcc atcgccatcc gaaccatgat tctcaaaaat caaagagcct atgtgcaggc
     1501 tggggcaggg attgtctacg actctatcgc ccaaaacgaa taccaagaaa ccattaacaa
     1561 agcaaaatct atgactagaa ttggagaact aagaccatga ttttattgat tgacaactat
     1621 gattctttta cctataactt ggcccaatac attgggaatt ttgcagaagt tcaggttctg
     1681 agaaatgatg attccaagct gtatgaagaa gctgaaaaag cagatggtct ggtcttttct
     1741 cctggtcctg gttggccagt tgatgctgga aagatggaag acatgatttg tgattttgca
     1801 ggcaagaagc cgattctagg gatttgtttg ggtcaccaag ccattgcaga agtctttggt
     1861 ggtaagctag gtttggctcc aaaagtcatg catgggaaac agagcaatat caactttgaa
     1921 gcgccatctg ttttgtatca aggtattgag gatggccgtg cggtcatgcg ttatcacagt
     1981 attttgattg aggaaatgcc agaagacttt gaagtgacag ctcgttcgac tgatgaccaa
     2041 gccatcatgg ggattcaaca taaaaaccta ccaatttatg gcttccagta ccatccagag
     2101 agcattggaa cgccagacgg cttgtcttct attcggaatt ttatcgagga ggttgtaaag
     2161 tgaggaaact aggatgaaag agattattga aaaactagca aaatttgaaa atttatcagg
     2221 tgtggaaatg acggatgtca ttgagcgtat cgtaactggg cgtgtaactg aagcgcagat
     2281 tgcttctctc ctcttagctc ttaagatgaa gggggaaaca cctgaagaac gtacagccat
     2341 tgcccaagtc atgagaggac atgcccagca tattccaact gagattcatg atgctatgga
     2401 caactgtggt acaggtgggg acaagtcttt cagttttaat atttccacaa ctgcagcctt
     2461 tgtcttggct ggtggcggta ttcacatggc caagcatggt aaccgctcga tttcttctaa
     2521 atctggttcc gcagatgtcc tcgaagcctt gggaatcaat cttgacctca aaccagctga
     2581 actaggtaag gtctttgata aaactggaat cgtctttctc ttcgctaaaa atatgcaccc
     2641 agctatgaaa tacatcatgc cagctcgttt ggaattggga attccaacga ttatgaactt
     2701 gactggtcca ctgattcatc caatggcttt agaaacacag cttcttggaa ttagtcgtcc
     2761 agaactccta gaaagtacag ctcaggtttt gaaaaatatg ggtcgcaaac gtgccatcgt
     2821 ggttgctgga ccagaagggt tggatgaagc tggcttgaac ggaacaacca agattgcact
     2881 tcttgaaaat ggcgaaatca gcttgtcaag ctttactcca gaggatttgg gaatggaaga
     2941 ctatgctatg gaagatattc gtggtgggaa tgctcaggaa aatgcagaaa ttttgcttag
     3001 cgttctgaaa aacgaagcaa gtccattctt ggaaacgaca gtcttgaatg ctggtcttgg
     3061 tttctatgct aatggtaaga ttgatagcat caaggaagga gttgccttgg cccgtcaagt
     3121 gattgctaga ggcaaggccc ttgaaaaact cagactgtta caggagtacc aaaaatgagt
     3181 caggaatttt tagcacgaat cttagagcag aaggcgcgtg aggtggagca gatgaagctg
     3241 gagcaaatcc agcctctgcg ccagacctat cgcttggcag aatttttgaa gaatcatcag
     3301 gactgcttgc aggtaatcgc tgaggtcaag aaagctagcc ctagtttggg agatatcaat
     3361 ctcgatgtgg atattgtgca acaggcccag acttatgaag aaaacggagc agtgatgatt
     3421 tcggtgttga cagatgaggt tttctttaaa gggcatttgg attatctacg ggaaatttcc
     3481 agtcaggtag agattccgac gctcaacaaa gactttatca tagatgaaaa gcaaatcatc
     3541 cgcgctcgca atgcaggtgc gacagttatc ttgcttattg tggcagcctt gtccgaagaa
     3601 cgcctcaagg aactgtatga ctacgcgaca gagcttggtc tggaagtctt agtggagact
     3661 cacaatctag ctgaactaga ggtagcccac agacttggtg ctgagattat cggggtcaac
     3721 aaccgcaact tgactacctt tgaggtcgac ttgcagacca gtgtagattt agccccttac
     3781 tttgaggaag gtcgctatta catttctgaa tctgccattt tcacagggca ggatgcggaa
     3841 cgactagccc catactttaa cggaattttg gtagggacag ctcttatgca ggcagaaaat
     3901 gtggcccaga gaatcaagga gttgcagatt gacaaaggtt aaaatttgtg gactatcgac
     3961 caaagaagcg gtggaaacag ccgtttcagc aggagccgac tatatcggtt ttgtctttgc
     4021 acctagtaaa agacaggtga ctttagaaga ggcagctgag ttggcaaagc ttattcctgc
     4081 agatgtgaaa aaggttggag tatttgtttc accaagtcgg gtagaactgc tggaagcgat
     4141 tgacaaagtt ggcttggact tggttcaagt tcacggtcag gtggcagctg atttatttga
     4201 gaatttgcct tgtgccagta ttcaggctgt gcaggtagat ggaaatgggc atgtccccaa
     4261 ttctcaggca gattatctac tctttgatgc ccctgtggca ggaagtggtc agtcctttga
     4321 ttggggtcaa ctggatacga ctgaactagc acagcccttc tttatcgcag gtggccttaa
     4381 tgaagataat gtagtaaaag caattcaaca ttttactccc tatgcagtag atgtatcgag
     4441 cggagtagag acagatggac aaaaagatca tgaaaagatt agaagattta tagagagggt
     4501 aaagcatggc atatcaggaa ccaaataaag atggatttta cggaaaattc ggcggacgtt
     4561 ttgtcccaga aacattgatg acagcagttt tggagttgga gaaggcctac cgtgaaagtc
     4621 aggcagaccc aagtttccaa gaggaattaa accagctttt gcgccagtac gtaggacgtg
     4681 aaactcctct ttattacgca aaaaacttga cccagcatat cggcggagcc aagatttatc
     4741 tcaaacggga agaccttaac catacaggag cccacaagat taacaatgcc ttgggacaag
     4801 tttggcttgc caaacgcatg ggtaaaaaga aaattatcgc agaaactggt gctggtcagc
     4861 acggtgtggc aactgcaact gctgcggccc tctttaacat ggaatgtacc atctacatgg
     4921 gtgaggaaga tgtcaaacgc caagccctca atgtcttccg tatggagctt ttgggagcta
     4981 aggtcgaggc cgtgacagat ggttcgcgcg tgctcaagga tgcggtcaat gcagcccttc
     5041 gttcatgggt ggctaatata gatgataccc actatatcct tggttctgcc ttggggcctc
     5101 atccatttcc agaaattgtt cgtgacttcc aaagtgtcat tggtcgagag gctaaacaac
     5161 agtaccgtga cttgacagga caaaatctgc cagatgccct agtagcctgt gttggtggtg
     5221 ggtcgaatgc catcggtctc ttccatccct ttgtagaaga tgagtcagta gccatgtatg
     5281 gagctgaagc ggctggattt ggtgtggata cggagcacca cgcagctacc ttgaccaagg
     5341 gtcgtccagg tgtccttcac ggttccctca tggatgtgct ccaagatgcc catggtcaaa
     5401 ttcttgaagc cttctctatc tcagcaggtt tggactatcc tggtatcggt ccagagcatt
     5461 ctcactacca cgatattaaa cgtgccagct atgtccctgt gacagacgaa gaagccttgg
     5521 aaggattcca actcttgtct cgtgtggaag ggattatccc agctttggaa tctagccatg
     5581 ctatcgcctt tgcggtgaaa ttggccaaag aacttggacc agaaaagtct atgattgtat
     5641 gcctatcagg tcgtggggac aaggatgtgg ttcaagtaaa agaccgcttg gaagcagatg
     5701 cagcaaagaa gggagaagct catgcctaag acactaacaa aaaaattaaa tgctattaaa
     5761 gcagctggaa aaggaatttt tgttccctat atcatggctg gtgaccacga aaaaggtttg
     5821 gatggtcttg ctgaaacaat tcacttttta gaagatttgg gtgtctctgc aattgaagtg
     5881 ggtattccct tttcagaccc tgttgcagat ggacctgtta tcgaagaagc tggcttgcgc
     5941 agtttagccc acgggacctc tacccaggct ttagttgaaa ccttgaaaac cattgaaaca
     6001 gagattccac tggtcatcat gacctacttc aaccccctct ttcagtacgg tgtggagaac
     6061 tttgtcaaag atctggctga tacagctgtg aagggcttga ttatcccaga cctgcctcat
     6121 gaacatgcca actttgtaga accatttttg gcagatacag atatcgcctt gattccttta
     6181 gtaagcttga ccacaggaat tgagcgccag aaagagttga ttgaaggggc ggagggattc
     6241 gtctatgccg ttgccatcaa tggggtaaca gggaaatcag gcaattaccg tgcagatttg
     6301 gacaagcact tggcgcaact tcatcaagtg gccgacatcc cagtcttgac aggttttggt
     6361 gtatctagtc aagccgatct ggaacgcttc aatgcggtgt cagatggtgt tatcgtcggt
     6421 tcgaaaatag taaaagctct ccaccaagga gagccgattc aggactttat caaacaagca
     6481 gtagcttacc aaaaataatt aaacaagtag caagtaagag gaaaggcacg aaaggaagtc
     6541 tttccttttt cttttgcagg agaaaggcta gttttatagt gaattgaatc tggaatatta
     6601 cactacggtt cctaaaatat tttcagaaag tgatttgaat ttcccaataa atttgttcag
     6661 tttttaattg attttactat agaagatata attttctaaa ttactctgac acagaacttt
     6721 aagaaaaagc taattattgt agtaatttct ttaagaaaat gatataattc attaaaaagt
     6781 atttggagga ggaagaatta tgttgaatca ggatctcttt gattcgcttg aggcacaaaa
     6841 aattgtagat actttgatga aagggcaaaa agattatgta gatgaacgtc tagaaaaaag
     6901 agagacaatg atagtgtcga atggttatgc atggacgcga cctaatcata ttgatactgc
     6961 atttgcatca gcagatttgt ttgagtataa attacaatta gcaggacaga cttggggata
     7021 tttagaattt gaaacaaata cagaaaaata tgggaaagta ttgttaatta taaagggtaa
     7081 gaagcgactt acgaaccaat ttcctttggt acaaaaaaat aagagtggct acttatttga
     7141 atacgctcag atgaatacac tttatcttaa tcaacattct tcctacaaaa atgatgaaga
     7201 tagtcattcc tttccaattc agatggagtt agtttctgat gaaatgattc aagaaattga
     7261 acaagctact aaaaattcga atatcgaaaa atttatgatt ttaacttatg aggcggactc
     7321 agaaaacaat attatatctg tagatgttgt tatgcctgat gcacgaactg gtcagttaca
     7381 cttgattcaa gatttgtctg agtatattca atcaagttcg taccatttcg aagaagctaa
     7441 atatcaagat attcctaatt tttcagaatt atcggaaaca gaagattttg aaattattcc
     7501 aagaatagaa aaacaagaag gtcaaaagta gtaaggagta tgtaaatgtt taatggtcgg
     7561 gtattgaaag aattacggct gttaaatggt ttaagtagag cagaattagc tcagagaatt
     7621 aatttaacgg aacaagccat ttggcagttt gagtccaacg aaacgaaacc taaattatca
     7681 accaaaatgc atttggccaa ccaatttcat gttgatttaa cttattttga acaggaagaa
     7741 gagagcattc gatttgattc ttctgtaatt gcctttagaa atgcagacct agcaacacgg
     7801 aaaacaatag atattcaaac tatgtattta cataaggtag atagtttgat tgattatttt
     7861 gaaagtttta taattatacc taatattata attcatgacc taagtaatgt agtgagtgaa
     7921 tcttatcata agggagaatc cattgaggaa ttggctcttt atgccaggga aaaattaggt
     7981 atttcaaaag ataatcatga tttgctttat aaattagaac gttcaggcat ctatatcgtg
     8041 gaacgattaa ttaatggcca agctgatgct tatagcgcat ggtcaaaatt gggaagacct
     8101 tatattgtgt tgggaacgaa taaatcatct gtacgtcgaa attttgactt agctcatgag
     8161 ctaggacata ttcttttaca taaatataaa gatatgaatg aagatggcga tcgtttggag
     8221 caagaagcaa attattttgc atcatgtttt ttattgccaa aagaagagtt tttagtcaaa
     8281 tttgaagaga gggttggcaa gcgtgtcagc aatcctgata gttatatttt attgaagtcg
     8341 gatttgaatg tttcgataca ggctttagag tatcgagctt ttaagttagg attattgact
     8401 ccaaagcaac attcttactt ttatcgtcaa attgcgcaaa aaggttacaa aatgattgaa
     8461 cctttggatg atcaaatttt tgttaaaaaa ccaagcaaag taaagagtat tctggacgtc
     8521 gttttgagta atcatctagt cagtctagcg actataatgt ctaaacaaag tattcgttta
     8581 cagtttataa gcgaaatatt ttcagtcgaa atgaaatttt ttgatcagta tcaagaagat
     8641 agaagaacag atcgatttga taacatcatc cctttgtaca aaagaaataa tttataattt
     8701 aactgtgaaa taatttcatg aaggacatat ggcagaaatt ggactttatc aaacaagcag
     8761 tagcttacca aaaataatca aacaagtagc aagtaagagg aaaggcacga aaggaagtct
     8821 ttcctttttc ttttgcagga taaaggccag gatacccgtc gcagaagcaa actgaatcaa
     8881 gatcagtaac tccgttacgc taaagacgag agcacaagaa gctaaaaaga ggaaatctcc
     8941 tgcgcccatg cggatatcga taaaatgagc caaaattcca agagcaagga aggagaccat
     9001 gaccagattc cagccagagg aagctattag gattaggtgg aaagtcatcc agaccagtaa
     9061 gggatattcc tgatggtgaa agtcgtagat acccaaggtc aaaccagcgg tgattaggac
     9121 gacttgcccc aaggaaagca attcccaaga gtaaagcaga aagaggagtc ctaagactaa
     9181 ttcaaagagg gcataccaga caggatagcg aactttgcag tagcgacagc gaaagcgatt
     9241 gaagacctgt gagagaatcg gaatcaaatc taagggacgc aagcgagtct gacaggaatc
     9301 gcagtgactg gctgaactga taatggattg ctctggaaaa cggtcaatga ccaaaccaag
     9361 aaaggaagcg agaatgctcc cgacgagaaa aaaataaaaa tcaatcatac ttatctattc
     9421 gtaaaaaatg ggagaaatag tataatggag aaacatagat aagatggtga ggaaaagatg
     9481 acaattcgtt ttgaagaaaa ggtgagtata gaaaacgctc agctcgtatg ccaatggtcc
     9541 aactccttag gcaaagtctt tcaagaacaa tggatgggac caaggattcc ttttccactg
     9601 acccttcaag ccttggaagg agtcttttca atctttgatg aacaagagtt tgtgggactt
     9661 atccagaaaa tcaggctaga agacagcaat cttcatatcg ggagattttt tatcaacccc
     9721 cagaaacagg agcaagactt aggtagccag gctttaagga aatttgttag tttggccttt
     9781 gaaaatgaag atatagatag tatttctcta aatgtcttcg aggcaaatca aagagctcag
     9841 aatctttacc aaaaagaagg atttgaaatt gttcaaatgg ttgaagcacc tgtacgaaaa
     9901 tatagtagat tgaaactaga atagtacacc tctgcttcta aaacattgtt agaaatcgat
     9961 ttgactgtcc cgatcgattc gtcctgttat tatttcattt tactatacat tatgaaaaag
    10021 gggagataga aagcaaacag ccttgaaatc aatgttgact gcgattatga acctgaatca
    10081 gcccctcaaa agggactgta tatgaatgaa cgttaccagt gtttaaaaac taaagaatat
    10141 caggcacttt tatcttccaa gggtagacaa attttcgcta aacgtaagat tgatatgaaa
    10201 tctgtctttg ggcagataaa ggtttgtttg ggttataaga gatgtcatct gagaggtaag
    10261 cgtcaagtga gaattgacat gggattcata ctcatggcca acaacctgct gaaatataat
    10321 aagagaaaga ggcaaaatta aaaagctaga gttccgcaat tgggaatctc tagctttttg
    10381 ttttctgaga atcatcttga ctcagactct tttgactaat gttaccaaaa agataaagta
    10441 acttttaacc ttatttatcg tcgccactca acatgttttt aacaccttct acagcacctt
    10501 ctacagcgtc tttagcgtct tcggcaactt cttttacttt agaaacaact ttttcagctg
    10561 ctccttcttt ttccattttt tcatcaccga tggctttccc aacaccttct ttaatagaac
    10621 ctttagcttg atttaatttt tcttctactg acatgataat ttctcctgtt atatttattt
    10681 tatcttagtt tacacgagtg ttttcgtgag atactacacc atttgctgca cctttaacag
    10741 cttctacacc ttcgctaact ttttcttgaa cagttgagaa accagatcca agaccagatt
    10801 tagctttttc gaattgttct gaagtgaatt ctcctgttga ttcagcaacg tcagatacgc
    10861 gatcttgaag gcttactgag tctgcttcat gctgttcttt agttttgatg tcgacaacgt
    10921 ttacgttgat ttcaacaatt tccaagtcag tcattttagc aacttctgaa gatacgattt
    10981 ctctgatttc tgaatataaa gctggaacat ttttttggta ctcaacaata acgtttaagt
    11041 caactgcaac ttgtgttt
//
LOCUS       DAVAAL010000056        10557 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVAAL010000056 DAVAAL010000000
VERSION     DAVAAL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10557
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.057"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            73..369
                     /locus_tag="VQM60_001220"
     CDS             73..369
                     /locus_tag="VQM60_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET1081088.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGVLKRSKKKSFYWYKDVIDSNGASIE"
     gene            490..1434
                     /gene="manA"
                     /locus_tag="VQM60_001221"
     CDS             490..1434
                     /gene="manA"
                     /locus_tag="VQM60_001221"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET1081089.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDTLLTKVPVKAG
                     DFFYVPSGTMHAIGTGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(1455..2786)
                     /locus_tag="VQM60_001222"
     CDS             complement(1455..2786)
                     /locus_tag="VQM60_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HET1081090.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALVAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMMP
                     LLFIVLPQLFDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILG
                     ILTFVFGIPSALSYGVMADVHIFGKIFFDAMDFLASNLLMPFGALYLSLFTGYIFKKA
                     LAMEELHLDERAWKQGLFQVWLFLLRFFVSSFQSSSLWSSLPNLCNQKGLE"
     gene            2869..3623
                     /locus_tag="VQM60_001223"
                     /pseudo
     CDS             2869..3623
                     /locus_tag="VQM60_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3643..4098)
                     /locus_tag="VQM60_001224"
                     /pseudo
     CDS             complement(3643..4098)
                     /locus_tag="VQM60_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
     gene            complement(4118..5293)
                     /locus_tag="VQM60_001225"
     CDS             complement(4118..5293)
                     /locus_tag="VQM60_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET1081091.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5352..6197)
                     /locus_tag="VQM60_001226"
     CDS             complement(5352..6197)
                     /locus_tag="VQM60_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET1081092.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6322..6879
                     /locus_tag="VQM60_001227"
     CDS             6322..6879
                     /locus_tag="VQM60_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET1081093.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            6898..7365
                     /locus_tag="VQM60_001228"
     CDS             6898..7365
                     /locus_tag="VQM60_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET1081094.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7454..8083
                     /gene="upp"
                     /locus_tag="VQM60_001229"
     CDS             7454..8083
                     /gene="upp"
                     /locus_tag="VQM60_001229"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET1081095.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8257..8847
                     /gene="clpP"
                     /locus_tag="VQM60_001230"
     CDS             8257..8847
                     /gene="clpP"
                     /locus_tag="VQM60_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET1081096.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            8926..9174
                     /locus_tag="VQM60_001231"
     CDS             8926..9174
                     /locus_tag="VQM60_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET1081097.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9276..10436
                     /locus_tag="VQM60_001232"
     CDS             9276..10436
                     /locus_tag="VQM60_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET1081098.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
BASE COUNT         3341 a         2100 c         2241 g         2875 t
ORIGIN      
        1 aatttcatat cactattata taatgctttt tgattttagt ttagtattat cgtagaaaat
       61 ggactcggta ctatggatac accggatgag aatggttatg tagcagatga ctatcggatt
      121 acttacttag aggcccacat caaggccatg cgagatgcca tttaccaaga cggggttgac
      181 ttgcttggtt atacgacttg gggctgtatc gatccggttt cagctggcac aggggaaatg
      241 aataagcgct atggctttat ctatgtagat cgagataatg taggaaacgg agttctcaaa
      301 cgtagcaaga agaagtcctt ctattggtac aaggatgtca ttgatagcaa tggtgcaagc
      361 atcgagtaga gcacatttgt ttactatttt tataaaatct tctccctgct ttataagatg
      421 taaaaaagag ttcaaatgga actctttttt tgctataatg aggggagaaa aatcagacag
      481 gagattaaga tgtcagaacc attattttta caatcagtta tgcaagaaaa aatctggggt
      541 ggtaccaagc tacgtgatga gtttggctac gacatcccaa gtgaaaaagt cggagaatac
      601 tgggctatct cagctcatct aaatggtgtt tctaaagttg ccaatggtcg ttacgaggga
      661 acagatcttg ctactttgta tgcggaacac cgtgaattat ttggcaatcg tccagaacct
      721 gtatttccac ttttgaccaa gatcctcgat gccaacgact ggctcagtgt ccaagttcac
      781 ccagacgatg cttatggact cgagcatgaa ggcgaactcg gaaaaacaga atgctggtac
      841 atcatcgctg cggatgaagg ttcagagatt atctacggtc acaatgccaa gtcaaaagaa
      901 gaactccgcc agcaaatcga ggacaagaac tgggatacct tgcttaccaa agtccctgtc
      961 aaggctggag atttcttcta tgtaccaagt ggcaccatgc atgctatcgg tactggcatc
     1021 ttgattcttg aaacccaaca gtctagtgat accacttacc gtgtctatga ctttgaccgt
     1081 aaggatgaca aaggcaactt gcgtgaactt catcttgaaa aatccatcga tgtcttgaac
     1141 attggtgagc cagcaaatag ccgtcctgta actgttaaag cagatgattt gcgttccact
     1201 ctccttgtat ctaatgattt cttcgcagtt tacaagtggg aaattactgg aaaagttgac
     1261 tttgaaaaga cagctgacta cagcttattg agtgtcttag ctggtcaagg tcagctaact
     1321 gttgacggga aaaattatcc aattcaaaaa gggagacact ttatcctacc aagtgatgtt
     1381 gaagcttgga ctctggaagg gcaaggtttg gaattgattg ttagccatcc ataaaaagaa
     1441 agggcctgag ttcactactc aagtcctttt tgattacata aattgggcaa tgaagaccac
     1501 aatgatgatg attggaatga cgaaacgaag aaacgaagaa ggaagagcca gacttggaac
     1561 agtccttgtt tccatgctct ttcatcgaga tggagttcct ccattgcaag agccttttta
     1621 aagatatagc ctgtaaaaag tgaaaggtag agagctccaa atggcatgag gagattggaa
     1681 gccaagaagt ccatagcgtc aaagaagatc ttaccaaaaa tgtgaacatc cgccatgaca
     1741 ccgtaagata gggctgaagg aatgccaaag acaaaggtca aaattcctaa aataacactc
     1801 catttggcac gtttgctgtt atcctggttg gtgatattgc ccacattgat ctccagcatc
     1861 acgacagaag aagtgacagt cgcaaagagg aagaggacgt agaaaatggt tccaaaaggc
     1921 atcttgtcaa agagttgagg taagacgata aagagcaggg gcatcatgac tgtaacccct
     1981 agtgagaggg caaagaaaga ttgtcccaga gcatagagga gaccagcgct agtcagtttt
     2041 gaaaagtctg gtttgaggaa gtaaagaacc ccttccatgg catttggcaa actgagagag
     2101 cgaccgatga taacaacaaa gatgataaag agcaggggca tcatgacttt cgaagctctt
     2161 tcaatccctt tttgaacccc acgtgataca ataaagatat ttaataagat aaaggctgct
     2221 tgagctacta gggcaatggc tggatttgaa atgattgaag taaataactg agcataatca
     2281 cccgttccac caagttggaa caatttccca aactcaatac ctagatagac tagaatccat
     2341 cctccgataa cactgtaaaa agataagagg ataaagaggg caaaggcgcc aatccaaccg
     2401 ataaagttgt acttgttatt cttgcccagt tttccaaagg ttttgatagc ggaaacgcca
     2461 gcactacggc caagggcaaa ctcagccagc aggagaggga aaccgattaa aatagtggaa
     2521 atgagaaaga ttagtaaaaa gcctccaccg ccattagcag cagtcatgta gggaaacttc
     2581 caaacggaac caagcccgat gaccgagcca gcagatgcta gaataaagcc aagtttcgac
     2641 ccccattgtg atttttctga catagttaaa ctctgatctc ctttttcaaa ataagaaaaa
     2701 gctacttgag ttgtcaaata gccttctgtt tgattgatag acttgactat cctattatgt
     2761 tttctaaggt ctgtcaagaa aaccattttc aagttttcac acctttctca aaaacgctta
     2821 actctgacct aaggggaggg gttatactat cattgtaagg aggaaatcat gtaccatata
     2881 aaagaagctg cgcagctttc aggtgtctct gtcaagaccc tgccctgcat cactatgaca
     2941 agataggact cttggtcccc ttaaagtcgg aaaacggcta tcgaacctat agtcaagagg
     3001 atttggaacg ccttcaggtc attctttact acaaatatct aggcttttct ttagagaaaa
     3061 tagcagagct gttaaaggaa gaaaggacag atttattgcc ccatttaact aggcagttgg
     3121 actatctaac tcgcgaaagg caacatctgg ataccttgat ttccaccttg caaaaaacta
     3181 ttcaagaaca aaaaggagaa agaaaaatga ccattgagga aaaattcacg ggatttagct
     3241 atcaagacaa tcaaaaatac caccaagaag cggtagagaa atatggtcaa gaagtcatgg
     3301 gacaagcgct tgaacgccaa aaaggtcacg aaaacgaggc tacggccgcc tttaaccaag
     3361 tctttcaaac tttggcacaa aatcttcaag ctggtttacc tgcaacagca accgaaaacc
     3421 aagagcaagc agccaagctc ttgcaagcca ttcgcactta tggatttgac tgctctattg
     3481 aggtattcgg tcatatcggt aaaggttacg tctacaaccc agagtttaag gaaaacattg
     3541 acaagtttgg ttctggaaca gcccagtaca cgtcagatgc cattgcggct tacgttcaga
     3601 caaatgcaga aaaaataggc taggaatttc ctagcctatt ttttacttca aatcataaag
     3661 ccagtcgtca ccgtttttgt agtaaaagaa ttcactgaga tcttcttcta gaaacacacg
     3721 aagcatatca gacatatcat cggttgcaag ttttagatga gaaagatttt caaagtcctc
     3781 ccactaaact ttcccttcgt ctgaagactg gagttcacca gtaaagtgtt ctgtcttgta
     3841 aaaaaggacg acataacgat aatccttgtc gtcataccag tttttgatac cacagagttg
     3901 gggtttggaa atgatcagac cagtttcttc tttcacttca cgaatgacag catcgacaaa
     3961 ggattcgcca cgttcaacat gaccaccagg aaaagtaatg ccagaccagt cgggactaac
     4021 tcggtcttgg accaggacct tatctccgtt tttaatcata cacatgttaa caaattcgac
     4081 tgcctctctt ctgttcattc ttcacaacct ttaatcttta atcataatgc agacttcccg
     4141 ccacccagcc ggtacagagg gcagaagtga tgttaaagcc acccgtgtgg gcattgatat
     4201 ccataacttc gcctgcaaag tggaggccag gtaccagctt actttcaagg gttttaggat
     4261 tgatttcctt gagactgact ccacccttgg taacaaagga ctttgcaagg gacatttttc
     4321 cagttacagg aattttaagt tctttaatgg actggacaag ttgttctcgt tccttttcag
     4381 tcagctgttt gactttttca ggatatcctt gtacaaaaaa ttcggccaag cgttctggta
     4441 acaaggtttt taaagcgttt ttcaaggatt tttcccgatt ttcttctaga aatgtaacca
     4501 agtccttctc agaaagttga ggcaaaacat cgagtgagag aacctcccca cctttgacaa
     4561 agctagacat gcgtagggca gcaggacctg acaaaccaaa gtgggtaaag agtaaatcat
     4621 gagtgatgac atgcttacca taacttaggg tcacatcgtc caaagaaatc ccttgtaagg
     4681 ctttatgtgg aaaatctgtt aataaaggac tttcagcagc ctcaagctcg gtgatggtat
     4741 gcttaaaatg gcgagcaatc tcgtgaccaa aaccagtcga accagtcgaa ggataagact
     4801 taccacctgt tgtgacaatg agtttctcac aagtgaaggt ttgatccgct gacttaagga
     4861 caaactggtc atctactttt ttaacagaaa cgatttctat ttgagtagca acttgaccac
     4921 ctagttcagt gattttcttt tccaaagctt cgataatagt ccgagacttg tcactggctg
     4981 gaaagacgcg tccgtggtct tcgaccttaa gtttaacacc attttctgta aaaaagttga
     5041 tgatgtcatg attatcgaac tgggagaaaa cactgtaaag aaagcgtccg tttccaggaa
     5101 ttccagctag caggttgtct aagctaccat tgttggtcac attgcaacgt cccccaccag
     5161 tcccagctaa tttttttcca agtttccgat ttttttcgat gaggagggtt ttctgtccat
     5221 aaaagctact ggaaatcgta gccatcatac cagcaggtcc cccaccgatg acaatagtat
     5281 caaaatgttt catagctcta ttgtaccaca aaaaaacaag agatgatggt cacctcttgt
     5341 caagaatgca attaatcaat ttcatagccc atcagcaaac cgccctcttc tgcatagaaa
     5401 ctgcagagac cagaggttgg tagaatttta atatccgctt gtgggaaggt ttcacggatt
     5461 cgctctgaga gctgttgaca acatttttcg ttattgcgtt gggccatgac aatacggcca
     5521 ccagcatatc cagcttttac taactcatca taggcagctt gaactgattt ctttgatccc
     5581 cttgcttttt gtagcaattc gagagttcca gtttcactag cttttccgac catacgaatg
     5641 ttgagaaggc caacgaccgt accgataagc ttgctcaaac ggccgttctt caccaagtta
     5701 tcgactttgg ctaggacaaa gagcaactta gttttttctt gataggcggt gatagcttca
     5761 accacttctt caaaagacaa gccctggtca atcaagtcat tcaatttttc tacgagtagg
     5821 tcaacttcac caccagcaga taaactatca atcacatgaa tcttagtgtc aggatggtct
     5881 tccagataaa tattctttgc tagttgagca ctattgtgac tgccagaaag ggtacctgtg
     5941 atggttacta ggaaaatgtt tttggcacct tcaaatgctc gcaaatagtc atctgggctt
     6001 ggacaagccg attttgaagc ttctgcagtt gcatacatgg tttccatcat ttggtcaata
     6061 tcgagactgg cgtcatcaac aaagacctga tcagctactt gaatggttaa ggggacactt
     6121 acaaaggttg tgttaatagc tggtgttggc agttgacgat aatcacaacc agagtcagca
     6181 ataatcttcc aagtcataga aattctccat ctttgtcagt tatacattga caaaggttct
     6241 gtcttttttt acaattatat catgaaaacc cttgaaacaa aagcatcatc cgtctgttgt
     6301 gacaagtaga aagaaaatgt tatgtctgaa cgtagaatct ctgaaaagtc tcttgaaaat
     6361 ctcagaaaat caaaccaaga atccaattta ttaaccagag aagccattga aacagccctc
     6421 ttgcaactct tggaaaaaaa ggaactgacc aagattagta tttctgaatt ggtcaaacgt
     6481 gcaggtgttt cgcgtgcggc cttttatcgc aattatgact ccaaagagga aattttagag
     6541 agcgtcttta aacgaactgt ccacaatatt atggaacagc tgcatcatta cgatttaaag
     6601 acagaccttt atttggtttg ggttcacctt ttccgggagg ccagaaagga agctagagtg
     6661 attcaattgg ccttggatta ccatctggaa aaaatctttg tccaagccat gcaggaattt
     6721 ctagaaaaat accatgggaa atcaaaaggt gtcagctctt atcttcattc cttctggagc
     6781 tcagccatcg tctctgtcct tctaaaatgg atcaaggatg gcatgaaggt accagctgaa
     6841 aagattgcag atttaggttt accatttttt aaaaaataga gaaaaaggag aaaagagatg
     6901 actgaaaaaa gactggcctg ggatgagtat tttgcagccc aagccctact aattgcgaat
     6961 cgttccactt gtaaacgtgc taaagtgggc gcgattctgg taaaagataa taaggttatt
     7021 tccactggtt acaatggttc ggtgtcaggg actgagcatt gtattgatca cgaatgtttg
     7081 gtcattgaag gccactgtgt tcgcaccctt cacgctgagg tcaatgctat ccttcaaggt
     7141 gctgagcgtg gtgttcccaa aggctttaca gcctatgtaa cccattttcc ttgtctcaac
     7201 tgtacaaaac aattgcttca ggtcggctgc aagcgtgtgg tttatatcaa ccagtaccga
     7261 atggacgact acgcccaata cctttatcaa gaaaaaggga cagaattgac ccatttacca
     7321 cttgagacag tacagacagc tcttaaagag gctgatctaa tgtaaaaatt atcaaaaata
     7381 aatggtttag aaagattttt aaaccgtttt ttggtataat aagaagaata aattgaaaga
     7441 aggaattcca aaaatgggaa aaattgaagt tattaatcac ccactgattc aacacaaatt
     7501 gtcaatcttg cgtcgtacag atacttctac aaaagctttt cgtgagctag tagatgagat
     7561 tgcaatgttg atggggtatg aagtacttcg tgatcttcca ctagaagatg tggaaatcga
     7621 aacaccaatt acaaaaacag ttcaaaaaca attggcaggt aagaaattgg ccatcgtccc
     7681 aatcttgcgt gcaggtatcg ggatggttga tggtctcttg agcttggttc cagctgctaa
     7741 agttggccac atcggtatgt accgtgatga agaaacactt caaccagttg aatacttggt
     7801 gaaattgcct gaggacattg accaacgtca aatttttgta gtagacccaa tgttggcaac
     7861 aggtggctca gcaatcttgg ctgttgattc tcttaaaaaa cgtggcgcat caaatatcaa
     7921 atttgtctgc cttgtatctg ctccagaggg tgtaaaagcc cttcaagaag ctcatccaga
     7981 tgtagaaatc tttacagcag ccttggatga acgtttgaac gaacacggtt atatcgttcc
     8041 aggtcttgga gatgctggag accgcttgtt cggtacaaaa taagatcgaa aagaagaatg
     8101 acttggaaaa gtatttccag tcacgaaagg aggttgggtt tttgtttctg tctaatgaaa
     8161 gcagagcaaa aatttgacct tttttgacca agatattata atagtcttat cttcagtcat
     8221 ttgaccaaca aaattaaaac tcaaaaggag aaatgaatga ttcctgtagt tattgaacaa
     8281 acaagccgtg gagaacgttc ttacgatatt tactcacgtc ttctcaaaga ccgcatcatt
     8341 atgctgacag gtccggttga agacaatatg gctaactctg ttattgccca attgcttttc
     8401 ttggatgccc aagatagtac aaaagatatt tacctttatg tcaatacacc aggtggttct
     8461 gtttcagctg gtttggcaat cgtagatacc atgaacttta tcaaggcaga tgtccaaacc
     8521 attgttatgg gaatggctgc atctatgggg actgtcatcg catcaagtgg ggcaaaaggc
     8581 aaacgtttca tgcttccaaa tgctgaatac atgattcacc aaccaatggg cggtacaggt
     8641 ggtggtaccc aacaaactga tatggctatc gctgcagaac acttgctcaa aactcgtaat
     8701 accttggaaa aaatcttggc tgaaaattca ggtcagtcaa tggaaaaagt ccatgcagat
     8761 gcagaacgtg ataactggat gagcgcccag gaaacacttg aatatggctt tattgatgaa
     8821 attatggcca acaattcatt gaactaatga tgatagaagg caaactcgac tgggtttgct
     8881 ttttttggta taatagggag agatttctta gaaagaggat ttatcatgtt tgaaaaagtc
     8941 aatcgctctg gcttgattat ctatctttac tataatcgtg atgccaaaaa actgcaggat
     9001 tatggagata ttacctatca ttccaagaaa catcgttact tacaactcta tgttccaact
     9061 caagaagtgg agcaattggt cggacgcttg agcaaggaaa aatttattaa aaaagttagg
     9121 gtttgtcata tccaagagtt ggaaacaccc tttgtgggca atctttatcg ataggaaaac
     9181 gttatcatcg aaaaaagctc aaggaaaaca ttgacaattt tctgataatt cggtatattc
     9241 ttaacagact atttaagaaa taaggagaca aaaagatgaa gaaaaaattt gccctatcgt
     9301 ttgtggcgct tgcaagtgta gcacttcttg cagcctgtgg agaagtgaag tctggagcag
     9361 tcaacactgc tggtaactca gtagaggaaa agacaattaa aatcgggttt aactttgaag
     9421 aatcaggttc tttagctgca tacggaacag ctgaacaaaa aggtgcccaa ttggctgttg
     9481 atgaaatcaa tgccgcaggt ggtatcgatg gaaaacaaat cgaagtagtc gataaagata
     9541 ataagtctga aacagctgag gctgcttcag ttacaactaa ccttgtaacc caatctaaag
     9601 tatcagcagt cgtaggacct gcgacatctg gtgcgactgc agctgcggta gcgaacgcta
     9661 caaaagcagg tgttccattg atctcaccaa gtgcgactca agatggattg actaaaggtc
     9721 aagattacct ctttattgga actttccaag atagcttcca aggaaaaatt atctcaaact
     9781 atgtttctga aaaattaaat gctaagaaag ttgttcttta cactgacaat gccagtgact
     9841 atgctaaagg gattgccaaa tctttccgcg agtcatacaa gggtgaaatc gttgcagatg
     9901 aaactttcgt agcaggtgac acagacttcc aagcagccct tacaaaaatg aaagggaaag
     9961 actttgatgc tatcgttgtt cctggttact ataatgaggc tggtaagatt gtaaaccaag
    10021 cgcgtggcat gggaattgac aaaccaatcg ttggtggtga tggattcaac ggtgaggagt
    10081 ttgtacaaca agcaactgct gaaaaagcat caaacatcta ctttatctca ggcttctcaa
    10141 ctactgtaga agtttcagct aaagctaaag ccttccttga cgcttaccgt gctaagtaca
    10201 atgaagagcc ttcaacattt gcagccttgg cttatgattc agttcacctt gtagcaaacg
    10261 cagcaaaagg tgctaaaaat tcaggtgaaa tcaaggataa ccttgctaaa acaaaagatt
    10321 ttgaaggtgt aactggtcaa acaagcttcg atgcagacca caacacagtc aaaactgctt
    10381 acatgatgac catgaacaat ggtaaagttg aagcagcaga agttgtaaaa ccataataga
    10441 aaaatgttga aatagggaat gagcctttga ctcactccct gtttcgatat ttaatactct
    10501 tcgaaaatct cttcaaactg cgtcaacgtc gccttggatt atatatgtga ctgactt
//
LOCUS       DAVAAL010000057        10376 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVAAL010000057 DAVAAL010000000
VERSION     DAVAAL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.058"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(70..300)
                     /locus_tag="VQM60_001233"
     CDS             complement(70..300)
                     /locus_tag="VQM60_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET1081099.1"
                     /translation="MSKKDKKIEIQVADAKVNVSKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(468..1112)
                     /gene="pcp"
                     /locus_tag="VQM60_001234"
     CDS             complement(468..1112)
                     /gene="pcp"
                     /locus_tag="VQM60_001234"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET1081100.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(1127..2050)
                     /locus_tag="VQM60_001235"
     CDS             complement(1127..2050)
                     /locus_tag="VQM60_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET1081101.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(2047..2736)
                     /locus_tag="VQM60_001236"
     CDS             complement(2047..2736)
                     /locus_tag="VQM60_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET1081102.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(2801..3505)
                     /locus_tag="VQM60_001237"
     CDS             complement(2801..3505)
                     /locus_tag="VQM60_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET1081103.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(3561..4583)
                     /locus_tag="VQM60_001238"
     CDS             complement(3561..4583)
                     /locus_tag="VQM60_001238"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET1081104.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(4715..7186)
                     /gene="parC"
                     /locus_tag="VQM60_001239"
     CDS             complement(4715..7186)
                     /gene="parC"
                     /locus_tag="VQM60_001239"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET1081105.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(7606..9549)
                     /gene="parE"
                     /locus_tag="VQM60_001240"
     CDS             complement(7606..9549)
                     /gene="parE"
                     /locus_tag="VQM60_001240"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET1081106.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            9685..10326
                     /gene="plsY"
                     /locus_tag="VQM60_001241"
     CDS             9685..10326
                     /gene="plsY"
                     /locus_tag="VQM60_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET1081107.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIMFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVVFGFAPVFCLYLAVVFFGTLYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILNNYDPLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
BASE COUNT         2978 a         2351 c         1838 g         3209 t
ORIGIN      
        1 agcctcttcg ctatctctcc tacaatcaac atggactatt atatcatgaa aatttcaaaa
       61 aaacaagact tattttgcta aattataatt ttcaatcaaa atttccacag ctttttccaa
      121 ttttttataa aaactatcga cattcccatt ctttataatg gcaaattgtc cgtctaattc
      181 ggcaatttct ccgataactt ttttaccgat agtcaatgta taaccttcaa aactgtcttt
      241 actaacatta actttggcat ccgctacttg aatttcgatt tttttatctt tcttgctcat
      301 ttcatacctc tcttcacttt ttctatttta caagaaaatc ctaaaactag caagaaaaaa
      361 ctctatatca ggcttctctg atatagagtt ttatgtaaac aacctcaatt gatttagcat
      421 agttaatagt tagtaaacaa gcataattga ctatcctttt taggttatca gtgagtctca
      481 ccgcctacta actgcaaatc cttatctttg tagtctacaa tagcttcaat agctacctct
      541 atgcctctta caatatcaca tagactcatt cctgcagtat tcggcttatt tactacttgt
      601 tccatcatat atggaatatg catgaaacct gctctcatat tagggaattt tttatccact
      661 aaataaagag cttggtacat caaatgattg caaacaaagg ttcctgcact attggataca
      721 actgccggaa gtccctgttt tttgatagct tgtaccatcg ctttgatagg taaactacta
      781 aaataggccg atgctccatc aatacgaatc ggtgtatcaa ttggttgatt gccttcgtta
      841 tcaggtatgc gagcatcatc ttgattaata gccactcgtt caggtgttaa gccggtcctg
      901 ccgcctgctt gtccaataca aagtacagca tctggttgat atcgtaatat ttctgcctct
      961 aaaacttctg acgacttata aaaaaccgtt ggaatttcta cccagcgaac ttcagcccca
     1021 ttaatctcag atggtaataa ttttacagcc tccaaagctg gattaatctt ttcacctcca
     1081 aaaggattaa aacctgtaac caatattttc attttatttt cctttactaa aatgcgagaa
     1141 agtacattaa gaatatgtga ataacaatca ttactagagc aacacctgct tgagccttta
     1201 taacgccatt ctgatctttc atatccatca atgctgctgg tagagcgtta aaattagcag
     1261 ccattggggt caataaggtc ccacaataac ctgctgtcat ggcaagagca ccagccacaa
     1321 ttggattagc tcccagagca aatacaaagg gaactccaac acctgctgta ataacggtga
     1381 atgctgcaaa agcatttccc ataatcattg tgaatagaac cattccaaga acataggcca
     1441 aaactcctat aaagcgacta tctgaaggaa caataccgct aatcagatga gagataacat
     1501 caccaacacc tgctacagta aaaatagccc ccaaagcccc taataattga ggaacaatcc
     1561 cacttgttga aacttgctga gtcattcgat tattttctga taacagactc ttagggtgac
     1621 tattggtaat cacaagaaca gaaattgtag caaacaaggc ggcaaggcta atcgaaatct
     1681 tgctaaattc tggaatcatt tgcgctaaga ccaacgcaag tattgccatc agcataactg
     1741 gaataaaaat tttatttttc aacctgttag attcaatatt ggctttcatt tcatctaagg
     1801 atggcaaggt tccgatacgg acttgcttaa acaatgttaa cagcgataat aggattacaa
     1861 taataccaat actcatattt ggcatatagg aaccacctat aaacgtaata gacaatagag
     1921 tccaaaatgc agatgtccca agtcgaactg ggtttgtttt atctttataa ctacaatagg
     1981 ctgtatggag aaattgacaa ccaatcacaa tataggtcaa ctctaatagt tgctttgcca
     2041 actctgtcat ttttgttctc ctcccctagt cttttttgat atcaattttt tatcaaataa
     2101 ataattgtaa atccccacta caataagtgt tataacagca acaataatag atgtagaagc
     2161 aatccctgca taattgcttt catagcctaa ctgatctaat gttcccccta tcaagaggac
     2221 tcccccagca cctacaaacg tattttgagc aaagaaattt ccaaaatttt cattcgcagc
     2281 cgcacgcgct tttattgtct catcttcaac ctctgttaac tttctaccta attgagactc
     2341 tgcagctgct tctcccatag gttgaaccaa aggtctgaca aactgagggt gtcctcctag
     2401 acgaattgaa aagaaaccag ctaactctcg aataaagaaa taaactgtat agaagtttcc
     2461 aactgtcaga cctttaatct ttcgaatcaa atcgattgat cgttgcttga gtccaaaggt
     2521 ttctgacagc cccacaagag gcaaggtaac cataaaaatc gtgagcactc gctgattgct
     2581 aaattctttt cccaaaatct ccaaaaattc aacgagagaa acacctgaaa ctaaagctgt
     2641 aaccaaacca gctaagacta ctgttgcaat tgtatcaaat tttaaaataa aacccacaac
     2701 aatgattgct attcctatta atctaatcca ctccatatca aactccttta tattcaaaat
     2761 gacagtattt taaaaatttt atcaagatca ataccattcc ttatttaatg tatttttcta
     2821 gttctttttg gtatttgcta ttggattcca atttttcttt ttgccatttt ttaaaaacct
     2881 cgttatattc ttttgttgta acaatatctt tttgcaattt cattccttta aagatatatg
     2941 gttccccctt aataccaact tgtgagtatg gttttgagaa tggtactacg ttacttacaa
     3001 ctggaaaacc accagatgaa gctgttggca tcaataatga actatctgtc gaccaagctt
     3061 gagctttggc atatttttca tatctcttct ctaggtcagt ggtctcagaa acagcatctt
     3121 ctaacaattt cttatattta tccaaaccag gtttagctac aacatcctta tcttttcctt
     3181 tcgtaatgcc aaggtgtttc atggcagaac cagattttgg atccataata ttcaagtaag
     3241 acgctggatc ttgatagctt ggagcccatc ctgtactgtt caaatcatag tctttttgag
     3301 aaggagcaac attgccgtat ttatcatttt ccatcaaacc atcaataaca tttccaataa
     3361 cgtctgtcct cgatgttcga gtcgctatac tgtagcccaa tgatgctgga tctactgcat
     3421 agacataaga aaatgttgtc ggtgcatctg cttctttatc agtttttcca caagccacta
     3481 aaatagctga cgtgctcagg accactcctg ctgttaagag ccactttttc tatttcataa
     3541 aaaatctcct ttggtttatt ttaatctact tttacaatcc aaccttctgg cgcttcaata
     3601 tcgccaaact gaatacccgt caattcatta tataatttac gcgtcacagg acctacttct
     3661 gtttcactat agaatacatg gaaatcatca ccatgttgaa tacctccaat tggagaaata
     3721 accgctgctg taccacaggc acctgcctct acaaaacggt caagattatc aattggaaca
     3781 tcaccctcaa taggagttaa tcccaagcga tgttctgcca aataaagcaa ggaatacttg
     3841 gtaatagatg gcaagataga tggactcaat ggtgttacaa attcattatc agctgtaatt
     3901 ccaaagaagt tagctgatcc gacttcttca atctttgtat gagttgatgg gtccagatag
     3961 ataacatctg agaaatggcg tgacttggcc atttttcctg gtaagagact tgcagcatag
     4021 tttccaccaa ccttagccgc acctgtacca tttggtgctg cacggtcgta ctcatcctga
     4081 atcaagaaat tggttgggac caaaccacct ttaaagtaat ttccaactgg catagcaaag
     4141 atggtgaaaa tgtactcttc tgccggtttt accccgataa tatctccgac accaatcaaa
     4201 agagggcgaa gatataaggt tccacctatt ccgtatggtg gtacgtattc ttcattcgca
     4261 cggacaactg ctttacaagc ttctacaaac atgtctgtcg gaacttgtgg catcaagaga
     4321 cggtcacatg tacgttgcag gcgtttagca ttttcatcag gacggaacag ttgaacactg
     4381 ccatccttag tacgataagc tttcaaacct tcaaatgctt gttgtccata gtgaagactt
     4441 ggagaagact ctgaaatatg caaagttgca tcctctgtaa gctctccttg atcccattgt
     4501 ccatttttga aatgagcaag atagcgataa ggtaatttca tataggaaaa accgaggttt
     4561 tcccaatcaa tcgttactgt catattccac tccttattct aaaaacctat ttcttatatt
     4621 ctacactatt tttctaaaat agcaagtata ttttgtaatt ttcagaaaat ttctccaata
     4681 aaaaccagct cttagaactg attcttcatt tcacttattt atcttcagta actacttcct
     4741 gaagataagc gtcaaaaact tcttcatctg aaatcgtgtc agaaatgaag cttccattgc
     4801 tagtgcgttc tgacaagttc aagtcttgca atcggctttc atagattgtt cctttattgg
     4861 attggacaag cagagtttgg tcgttcacat ccacttccgt actgaagaaa tcgccaacaa
     4921 atccttgctc tgcaactgct cctgccaaga agacacgatg cggtttgttt ttcaactcac
     4981 gcaagacttg taatcctcgt ttggcacggc tggttgctag aatttcctca atggaaacac
     5041 gtttcaagct tccacgctgg gtcaagaggt agaaggacga agtattacag ataaagccag
     5101 attggaggac atcatcttct ttcaaattca tagccttgac acctgctgcc ttagcaccga
     5161 caaccggaac ctcttcgata ttgaaacgca gggcataacc attttgacta accaagacaa
     5221 catcatctag tttaatcgga gccactgcta caatctgatc tgtatcgtct ttgagcttag
     5281 catacttgac agacttagat ctataggtcc gccatggagt gaattctttt cgctctaccc
     5341 gtttgatttg accaaggcga gtcactgcaa agtaggttgt cgcatcgtca aactgatcca
     5401 gtacttccac ataaaggatt tcttcattcg tttcaaagtt tgtgatggtt tggctcagat
     5461 gctctccgat gtccttccaa cgaatatctg ccaactcatg gattggtctg tagatgacat
     5521 ttccaagact tgtgaacatc aagaggtgct gggttgtctt ggcagattga acaaaaatca
     5581 aacggtcatc atcacgcttg ccaatttctt ccaaggtgga agccgcaaag gaacgtggac
     5641 tggtacgctt gatgtaacct gccttggtca cgctgacgta ggtatcttcc tcagcgataa
     5701 gactagctgt atcaatctca attgctttcg cagtgtcttc taaagaactc aaacgaggag
     5761 ttgcaaattt cttcttgacc tcacgaagtt ctttcttcat gagattgtac atagtccttt
     5821 catcaccgat aatagccgcc agcatagcaa tcttctcacg aagctctgct tcttcttcct
     5881 gcaagacaac cacatcggta ttggtcaaac ggtacagttg caaagttacg atagcctcag
     5941 cctgttcttc cgtaaaatca tagctaactt tgaggttttc cttggcgtcc gccttattct
     6001 cagaagcacg gataagagca atgacttcat ccaaaatcga aatcacacga atcaaacctt
     6061 cgacgatatg gagacgtttc tcagcctttt ctttgtcaaa gcgtgaacgc gccaaaatca
     6121 cttctcgacg gtgagcgatg tagctagaca ggattggaac aatcccaacc tgacgaggtg
     6181 tgaaattgtc aatcgccacc atattaaagt tgtagttgat ttgtaggtcg gtgtacttaa
     6241 ataagtagtt gagaacaagc tcagtattag cgtctttctt aagttcgata gcgatacgaa
     6301 gaccatcacg gtcagactca tcacgaacct cagcaatccc agctaccttg ttattaacac
     6361 gaacatcatc gattttcttg actagattgg ccttattgat ttcataagga atctcagtaa
     6421 taacgatttg ttccttacca ccttttagct tttcaatttc agtcttggaa cgaacaacca
     6481 cgcgcccttt cccagtctca taagctttct tgatttcatc acgaccctga ataatagccc
     6541 ctgtagggaa gtctggtcca ggcaagaatt ccatgagttt atcaatcttt gcagttgggt
     6601 ggtcaatcat gtaaactgca gcatctatga cctcagctaa attatgggga ggaatgtctg
     6661 tggcataacc agccgaaatc ccagtcgaac cattgaccaa gaggtttgga aaggctgctg
     6721 gcaagaccgt tggttctttc tccgtatcgt caaagttcca tgcaaaagga actgtctttt
     6781 tctcgatatc ctgaagaagg tagcctgcaa tttcagacaa acgtgcctca gtataacgca
     6841 tagccgcagg aggatctccg tccatagaac cgttattacc gtgcatttca actagaatct
     6901 cacgattttt ccagttctgt gacatacgaa ccatggcatc atagatagaa gaatccccgt
     6961 gtgggtggaa attccccatg atgttcccga ctgacttggc cgacttacgg tagctcttgt
     7021 caaaagtatt gctatcctta ttcatagaat aaagaatacg gcgctgaacc ggcttcaacc
     7081 catcacgaat atctggcaaa gcccggtctt gaataatgta cttggagtag cgaccaaagc
     7141 gctctcccat gatgtcctcc agggacatgt tttgaatgtt agacataaga tacaaagccc
     7201 ataaaatacc aagtgaaaat agaaaattct tgaagtaagc aaactcacaa gagaatttat
     7261 ctttttcaca cagtatctag ggcgtgttca actcctttca aagaatgtag agtaggtttt
     7321 tatgcagtaa aagatatttt acgggaattc gtcccgtgtt cagttacgat aagtaaccaa
     7381 actatcctgt ttgtattttt caatatgaaa atatggtttt ccaaaattag tcttagtttg
     7441 tgtcttagcc gctcccttaa acgcctcttt gagataagca ctcatagcag attcttcatt
     7501 aataatcctg caattttttc aaaccaagat tttcaaactg ctttttcaca tagtcattca
     7561 catccgactc taatttccag tttactaaca tattattttc tttcattaaa acactgtcgc
     7621 ttcttctagc gtaaacttga cattatcttc aatccattta cggcgtggtt ctaccttatc
     7681 tcccatgaga acattgacgc ggcgttcggc gcgcgctaaa tcttcaattg tgacacggat
     7741 gagggtacgt gtttctgggt tcatggttgt ttcccagagc tggtccgcat tcatctcacc
     7801 aagtcctttg tatcgttgga gggtagcgcc tttaccgaac tgtttacgga gttcttctag
     7861 ttctccgtcc gtccaagcgt aggccacttc ttctttcttg cctttacctt tggacatctt
     7921 gtaaagaggt gggagggcaa tatagacatg acctgcctcg actagcggac gcatgtaacg
     7981 gtagaaaaat gtcaagagca aggtctggat atgggcaccg tcggtatccg catcggtcat
     8041 gataatgatc ttatcatagt tggcatcttc aatagagaag tctgctccaa cacccgcacc
     8101 aatggtataa atcatggtat tgatctcttc atttttgagg atatccgcca tcttggcctt
     8161 ggctgtattg acaaccttac cacgaagagg tagaatagcc tggaacttgc ggtcacgacc
     8221 ttgtttggca gaaccaccgg cagagtcccc ctcaactaga tagagttcat tcttagcagg
     8281 attcttagat tgggctgggg tcaatttccc agacaacaag cccttatctt tcttgttttt
     8341 cttcccattt cggctctcat cacgcgcctt acgtgctgct tcacgagcat cacgggcctt
     8401 gatagccttg cggatgaggt tagaagctaa ttccccattt tccataagga aaaaggtcaa
     8461 cttatcagcc actattccat ccacaactgg gcgagctagg gggcttccta gtttatcctt
     8521 ggtctgtcct tcaaactgca agtgttcttc aggaactaag atagaaagaa cggccgctag
     8581 tccctcacga tagtctgaac cttcaaggtt tttatctttt tccttgagaa gacctgtttt
     8641 acgtgcatag tcattcatga ccttggtaat ggcagacttg agtcctgtct cgtgcgttcc
     8701 accgtccttg gtgcgaacgt tattgacaaa ggatagaatg ttatctgaga atccgtcatt
     8761 gtactggagg gctacttcca cttgaaaacc attgtcttcc ccttcaaagt aaagaactgg
     8821 cgtcaagatt tccttatctt cgttgagata agaaacaaaa tcttgtactc cattctcata
     8881 gtggaactca atcgcttcat ctgttcgctt gtccgttaaa gacaaggtca catttttcaa
     8941 gagaaaggct gattcattaa ggcgctctga aatggtattg tacttgaaat ctgtcgtaga
     9001 aaagatagtc gcgtcaggca taaaagtaac tttggtgcct gttttagact tgggtgctgt
     9061 accgattttc ttcaaagtcg tgacaggttt tccaccattt tcaaaacgtt gcttgtaaac
     9121 tgcgccatca cgggtaattt caacttctaa ccagctagaa agggcgttaa caacggaaga
     9181 acccactccg tgaagtccac ctgatgtctt atagccacct tgaccgaatt tccctccggc
     9241 atgaagaatg gtaaagataa cctcaacagt tggaattccc atagcgtgca tacctgtcgg
     9301 catcccacgt ccatggtctt gaaccgttag actaccgtct ttattgatgg ttacatcgat
     9361 acgatcacca aacccagaca aggcttcatc gactgcatta tcaacgattt cccaaactag
     9421 gtgatgaaga ccagcgccat cggtcgatcc aatatacatc cctggacgtt ttcggaccgc
     9481 atccaaccct tctagcacct gaatagcatc atcattataa ttgttaatat tgatttcctt
     9541 ttttgacaca aggaacctcc tattcgttca tctttactat tctacaggtt ttccaaggat
     9601 tttgcaaaat ttttctttct ccgatgtgac aattttagca gagattctct gcttttcttt
     9661 cccaattcat gatataatag gagtatgatt acaatagttt tattaatcct agcctatctg
     9721 ctgggttcga ttccgtctgg tctctggatt ggacaagtat tctttcaaat caatctgcgt
     9781 gaacatggtt ctggaaatac cggaacgacc aataccttcc gcattttagg caagaaagct
     9841 ggtatggcaa cctttgtcat tgactttttc aaaggaaccc tagcaacgct tcttccgatt
     9901 atgtttcatc tacaaggcgt ttcgcctctc atctttggac ttttagctgt aatcggccat
     9961 accttcccta tctttgcagg atttaagggt ggtaaggctg tcgcaaccag tgctggagtg
    10021 gttttcggat ttgcgcctgt cttctgtctc taccttgcag ttgttttctt tggaaccctc
    10081 tatctaggca gtatgatttc actttccagt gtcacagcat ctatcgcggc tgtcatcgga
    10141 gttctactct ttccactttt tggttttatc ctgaacaact atgaccctct cttcatcgct
    10201 attatcttag cacttgctag tttgattatc attcgtcata aggacaatat agctcgtatc
    10261 aaaaataaaa ctgaaaattt ggtcccttgg ggattgaacc taacccatca agatcctaaa
    10321 aaataaaatg ccagttctgt gaactgcacc ccaaaagtta gacaattaat ttatcc
//
LOCUS       DAVAAL010000058        10363 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVAAL010000058 DAVAAL010000000
VERSION     DAVAAL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.059"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            49..537
                     /locus_tag="VQM60_001242"
     CDS             49..537
                     /locus_tag="VQM60_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET1081108.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            534..1721
                     /locus_tag="VQM60_001243"
     CDS             534..1721
                     /locus_tag="VQM60_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET1081109.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEVA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEESEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            1714..1938
                     /locus_tag="VQM60_001244"
     CDS             1714..1938
                     /locus_tag="VQM60_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET1081110.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            2095..3441
                     /gene="asnS"
                     /locus_tag="VQM60_001245"
     CDS             2095..3441
                     /gene="asnS"
                     /locus_tag="VQM60_001245"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET1081111.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            3594..3884
                     /locus_tag="VQM60_001246"
     CDS             3594..3884
                     /locus_tag="VQM60_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET1081112.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            3896..4366
                     /gene="ssbA"
                     /locus_tag="VQM60_001247"
     CDS             3896..4366
                     /gene="ssbA"
                     /locus_tag="VQM60_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET1081113.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            4398..4637
                     /locus_tag="VQM60_001248"
     CDS             4398..4637
                     /locus_tag="VQM60_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET1081114.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            4932..6332
                     /locus_tag="VQM60_001249"
     CDS             4932..6332
                     /locus_tag="VQM60_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET1081115.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIGKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            6334..6693
                     /locus_tag="VQM60_001250"
     CDS             6334..6693
                     /locus_tag="VQM60_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET1081116.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHYLKKKK"
     gene            6747..7496
                     /locus_tag="VQM60_001251"
     CDS             6747..7496
                     /locus_tag="VQM60_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET1081117.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPHSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            7486..7785
                     /locus_tag="VQM60_001252"
     CDS             7486..7785
                     /locus_tag="VQM60_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET1081118.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            7856..8791
                     /locus_tag="VQM60_001253"
     CDS             7856..8791
                     /locus_tag="VQM60_001253"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET1081119.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            8878..9390
                     /locus_tag="VQM60_001254"
     CDS             8878..9390
                     /locus_tag="VQM60_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET1081120.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAAFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERDLHDSDF"
     gene            9415..10023
                     /locus_tag="VQM60_001255"
     CDS             9415..10023
                     /locus_tag="VQM60_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HET1081121.1"
                     /translation="MFGELIRYLDQYEDVILREIKSQFPDVAVDKLMEEYIKASLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDRKYELAIDFDKERLTFYLA"
     gene            10124..10318
                     /locus_tag="VQM60_001256"
     CDS             10124..10318
                     /locus_tag="VQM60_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET1081122.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
BASE COUNT         3113 a         1943 c         2366 g         2941 t
ORIGIN      
        1 ttcttatatt cacttgtaat aaagcttaat agatggtaaa atagacgagt gaaaaaaaga
       61 caaaacaaag caaaaaataa tctactgtgg cagtatggtc tagggatgac gattttgttt
      121 gtggttatca gtgcttcctt tctgtatatg gtttctctta gcatgaaacc ctatcaaaca
      181 gctaaaagtg aaggagaaaa attagctcag cagtatgcag gattagagca ggccgatcag
      241 gttgatttat acaatggctt ggaatcttat tacagcgttc ttggtcgtaa taaacagcaa
      301 gaagcacttg ctgttctgat tggaaaagat gatcataaga tttacgttta tcagctaaat
      361 cagggtgttt cacaagaaaa agcagaaacg gtttctaagg aaaagggagc tggcgaaatt
      421 gacaagatta tctttggtcg ttatcaagat aagccaatct gggaagtcaa gtcaggatct
      481 gatttttatc tagtagattt tgaaacagga gcattggtca acaaggaggg cctatgaaat
      541 tatccaaccg tgttttagaa atggaagaaa gtgtgacttt ggctgctgga gcccgtgcca
      601 aagcactgaa ggctgagggc agagatattt tgtctctaac cttgggtgag ccagatttca
      661 ctactcccaa aaatatccaa gatgccgcca ttgcatcgat tcgagatggg cgtgcttctt
      721 tttatacagt aacctcaggt ctgccagagc ttaaggcggt ggtcaatagc tactttgagc
      781 gcttttacgg ctattctgta gcgtcaaatc aagtgacagt cgctgcggga gccaaatatt
      841 ctctctatac cttctttatg gctgtggtca atccaggtga tgaagtcatc atcccaaccc
      901 catactgggt tagctatgga gatcaggtca agatggcaga aggcgttccc gtctttgttt
      961 ctgctaagga agacaatcac tttaaggtga ccgtagagca gttagaagta gctcgcactg
     1021 acaagaccaa ggttttggtg ctgaattcgc catctaatcc cacaggtatg atttacaccc
     1081 gtgaggaact cttggcaatt ggaaactggg ctgtagaaaa tgatattctc atcctagcag
     1141 acgatatcta tggccgcttg gtttataatg gtcatgagtt cacaccgatt tctagcctat
     1201 cggaagcgat tcgcaagcaa acagtggtca tcaatggtgt gtctaaaacc tatgccatga
     1261 ctggttggcg gattggttat gccgttggag aagcagacat tatcgctgcc atgtccaaga
     1321 ttgcaggtca aacaacttcg aatccgtcag cagtagccca atatgcagca gttgaggctc
     1381 tatcaggtga gcaagatact gtagaaagca tgcgtcaggc ctttgaggaa cgtcttaata
     1441 ccatctatcc cctccttgca gaggtgccag gatttgaagt ggtcaaaccg caaggggcct
     1501 tctatctctt cccaaatgtc aaaaaggcca tggagatgaa aggctacacg gatgtgacag
     1561 actttacaac tgctatctta gaagaatccg aagtggcctt ggtaacagga gctggatttg
     1621 gagcaccaga aaatgtccgc ctcagctatg cgacagacct agacacgctt aaagaagcag
     1681 tcgaacgctt gaaagcattt atgggtagtg agaatgattg atcattttga gattaaggta
     1741 aaggatttac aaatttcaga aggattttat aggagttttc tcgctccttt ggactataaa
     1801 ttggctttta agactagttc tctaattagt tttctatccc cgaacagccc tcatcctggt
     1861 ggtgattttt ggctgactca gggaacacaa gatcctgttc attttgcttt tttagcagaa
     1921 aataaggaag aagtttaggc ttgttatgag gctggcttag aggcaggtgg gcgagacaat
     1981 ggggctcctg gttatcgaag tgagcgtccg atttactatg ctgcttttat gattgacctg
     2041 gatgggaaca atatagaggt ggtttgccat aaagaataaa aaagaaaagg aaaaataatg
     2101 acaaaacgtg taacgattat tgacgtaaaa gactatgttg gtcaggaagt gacgattggc
     2161 gcttgggttg ccaataaatc aggaaaaggg aaaatcgctt tcttacaatt gcgtgatgga
     2221 acagccttct ttcaaggtgt ggcttttaaa ccaaactttg tcgaaaaatt tggtgaagaa
     2281 gtgggacttg agaagtttga tgttatcaaa cgcttgagcc aagaaacgtc tgtttatgtg
     2341 acaggtattg tcaaagagga cgaacgttct aaatttggct atgagttgga catcacagac
     2401 atcgaagtga tcggtgaatc tcaagactac ccaatcacac caaaagaaca cggaacagac
     2461 tttttgatgg ataaccgtca cttgtggcta cgctctcgta agcaagtagc tgtgttgcaa
     2521 atccgtaacg ctattatcta tgcaacttat gagttctttg acaagaacgg ttttatgaag
     2581 tttgacagcc caattctttc aggaaatgcg gcagaagatt ctacagaact ctttgaaact
     2641 gactacttcg gaacgccagc ctacttgagc caatcaggtc aactttacct agaagcaggg
     2701 gctatggctc ttggtcgtgt ctttgacttt ggtccagttt tccgtgctga aaaatcaaaa
     2761 acacgccgtc acttgactga gttctggatg atggatgctg agtactcata cttgacacat
     2821 gatgagtcgc ttgacttgca agaagcttat gtgaaagctc ttctacaagg tgttcttgac
     2881 cgcgcgcctc aagccttgga aaccttggaa cgtgatacag aactcttgaa acgctacatt
     2941 gcagagccat tcaaacgtat cacttacgat caagccattg acctcttgca agagcatgaa
     3001 aatgatgaag atgctgacta cgagcatctt gagcatggtg atgactttgg gtcaccacac
     3061 gaaacttgga tttcaaacca ctttggtgtg ccaacatttg tcatgaacta tccagcagcc
     3121 atcaaggcct tctacatgaa accagttcct ggaaatccag agcgcgtgct ttgtgcagac
     3181 ttgcttgctc cagaaggcta tggagaaatt atcggtgggt ctatgcgtga ggaagattac
     3241 gatgcccttg tcgctaagat ggatgaactt ggcatggatc gtacagaata tgaattctac
     3301 cttgaccttc gtaaatacgg tacagttcca cacggaggat ttggtatcgg tatcgaacgt
     3361 atggtaacct tcgcagcagg aacaaaacat atccgtgaag ctattccatt cccacgtatg
     3421 ttgcaccgta tcaaaccata agaacaggaa aacgcccatg tggcgttttt tcgaattcta
     3481 gtgtttttac ttgcgtaaaa aaattttttc tagtataata gtttattgtg agcgaacctc
     3541 acttacccct tgcaaagtct tggggtcatt agaccaaaag gaggaacata tcaatggcta
     3601 aatacgaaat tctttatatc attcgtccaa acattgaaga agaagctaaa aacgctttgg
     3661 tagcacgttt tgactctatt ttgactgaca acggtgcaac tgttgttgaa tcaaaaactt
     3721 gggaaaaacg tcgtcttgca tacgaaatcc aagatttccg tgaaggactt taccacatcg
     3781 ttaacgttga agcaaatgac gatgcagctc ttaaagagtt tgaccgtctt tcaaaaatca
     3841 acgctgacat tcttcgtcac atgatcgtca aaattgacgc gtaagaaggt aaactatgat
     3901 taacaatgtt gtacttgtag ggcgtatgac acgtgacgct gagttgcgtt ataccccatc
     3961 aaatgtagca gttgcgactt ttactcttgc agtaaaccgt acatttaaga gtcaaaatgg
     4021 tgaacgtgag gctgatttta tcaatgtcgt tatgtggcgc caacaggctg aaaatcttgc
     4081 taactgggct aaaaaaggct cacttatcgg ggtgacaggt cgtatccaga ctcgtagtta
     4141 cgataaccag caaggacaac gtgtctacgt gacagaggtc gtggctgaga atttccaaat
     4201 gttggaaagc cgtagtgtgc gtgagggtca cacaggtgga gcttactctg caccaactgc
     4261 aaactattca gcacctacaa attcagtacc agacttttca cgtaatgaaa atccatttgg
     4321 agcaacaaac ccattggata tttcagatga tgatttacca ttctaatgga caactaaact
     4381 tataaaggag aaaaaacatg gctcaacaac gtcgtggcgg attcaaacgc cgtaaaaaag
     4441 ttgattacat cgcagcaaat aaaattgaat atgttgatta caaagatact gagcttctta
     4501 gccgtttcgt ttcagaacgt gggaaaatcc ttcctcgtcg tgtaacagga acttcagcta
     4561 aaaaccaacg taaagtaaca acagctatca aacgcgctcg cgtaatggct ttgatgcctt
     4621 tcgtaaacga agattaaaga aaagacccgg ttgggtctct ttttcatgag cttgaaaaca
     4681 agaaagcgaa ttaagacccc aacgggtctt ttttcacaag ctttgaaatg agagagcgag
     4741 ttaggctcag tggctccctt tttacgagtt tgaaaacaag agaacacatt acaagcacga
     4801 tggagctttt tcataaatat gtctcttttc tcatccaaga aattaaaata aaaatgataa
     4861 actttttgaa aataggggat ttgcctactt tatgatataa tggaaagagt tagattgaaa
     4921 gaggtagtga gatggacgcg aaattaagat acaaggcaaa gaagatcaag attgtctttt
     4981 ttgatattga tgatacattg cggaattcaa agacagggtt tattccaact acaattccca
     5041 ctgtttttaa acagttgcgt gaaaaaggaa ttttaacagg aatcgcctct ggacgtggca
     5101 tttttggtgt tgttccagag attcgtgatc tcaagcctga cttttttgta actttgaatg
     5161 gggcttatat cgaagataaa aaaggtcagg tcatttatca gcatcagatt ggaaagtcag
     5221 atgttgagga gtatatctct tgggctaagc aagaaggaat tgagtatggc ttggttggga
     5281 gtcatgatgc caagttgtcg actcgcaccg atatgatgag tgaagctatc aatccaattt
     5341 atcccgactt agatgtagat cccgatttcc atgaaaaaga agatatctat cagatgtgga
     5401 cttttgaaga taagggagat gacttgcact tgcctgacag tctctcagac aaacttcgca
     5461 tggttcgttg gcatcaacat tcgtctgata ttgtgcctat ttcaggctcc aaagcgacgg
     5521 gggtggaaaa ggttgtggaa caccttggct tgaaaccaga gaaggtcatg gtttttggag
     5581 atggtctcaa tgacttggaa ctctttgatt atgctggaat cagcgttgcc atgggaattt
     5641 ctcatgataa aatcaaagaa aaagcagatt atattacaaa aacattagaa gaagatggca
     5701 tttttgatgc cttagaagta tttggtatgg tagaaaaaga attgcatttt ccacaagtag
     5761 acattgaaac agtagaaggt cctcttgcga ctattaagac caatcacgga gacttacgta
     5821 tcaagctttt ccctgaacat gctcctaaaa cagtggctaa ctttgtatct ctttcaaaag
     5881 atggctacta tgatggtgtc attttccacc gtattatcaa ggactttatg atccaaggtg
     5941 gagacccaac tggaactggt atgggtggcg agtcaatcta cggcgaatct tttgaggatg
     6001 aattctcaga agagctttac aatatccgtg gtgctctttc catggcaaat gctggcccaa
     6061 ataccaacgg cagccagttc tttatcgtgc aaaaccaaca cctaccttat tctaagaaag
     6121 aaattactcg tggtggttgg ccagaaccga ttgcagaaat ctatgccaat caaggtggga
     6181 cacctcacct agaccgccgt cacacggttt ttggtcagtt agctgatgaa gcatcttacg
     6241 ctgtcttgga tgccattgct gctgttgaga caggagctat ggacaagcca gttgaagatg
     6301 ttgtaattga aactattgaa atcgaggact aagatgaaaa tcggtgataa gctaaagggg
     6361 cgtattacag ggattcagcc ctacggtgcc tttgttgagt tagagacggg tgatacgggg
     6421 ctgattcaca tttcagaaat tcggacagga tttattgaaa atattcatga aaccttgaaa
     6481 gtcgatgaag aggttcaagt tcaggtagtg gatttagatg aatttacggg gaaagccagt
     6541 ctttctatcc gcactttgga ggaagaaaag taccagtttc ctagacggag acgcttttca
     6601 agcgaccgct ttaactacgg ttttgctccc tttcgacgaa tgctaccaat ctggactgga
     6661 gaagccctgc actatttaaa aaagaagaag tagtttagag ttttatattt tttgtaatgt
     6721 taatataaat ttgctataat aggaccatgg aagaagaaca attattaaaa tcaggagagc
     6781 gcattaacca gctcttttcg acagatatca aaatcattca aaatagagag gtttttagct
     6841 attcggtgga tagtgttctc ttatcacgat ttccacgttt tcctaagaag gggttgattg
     6901 tagatttctg tgctgggaat ggagcagtgg ggctttttgc tagcactcgt actcaagcac
     6961 agatattgtc tgttgagatt caggagcgtt tggcggatat ggctgaacgc tctgtccgtt
     7021 tgaatggatt agaggagcaa atgcaggtca tctgcgatga tttgaaaaat atgcctgctc
     7081 acattcaggg gagtaaggtg gatatgattt tgtgtaatcc accctatttc aaggtggatc
     7141 ctcattctaa tctgaacgag agtgaacatt atctcttggc tcgacacgaa atcacgacta
     7201 atttggaaga aatctgtcat agtgcccaga gtattctcaa gtctaatggg cgtttggcca
     7261 tggttcatcg tcctgatcga cttctggata ttttggatac gttaaaacga cataatctag
     7321 cccctaagcg cctgcagttt gtttatccaa aaagagaaaa ggaagccaat atgcttttga
     7381 ttgaggcgat caaggatggc tcaacaagtg gctttaaggt cttacctcct ttgattgtcc
     7441 acaatgatga tggctcttat acgcccgaaa tcgaagagat ttattatgga tcataaggct
     7501 tatatgtacg ttctggagtg tcgtgatgga tcctactata taggctatac gactgatgtg
     7561 agaagacgcc tcgctatcca caatagtggg aagggagcca aatatacacg agcacgcttg
     7621 ccagtcaaac ttatctatgc tcaaggtttt gccagtaagg aagaagccat gtcggctgaa
     7681 gctcttctca agcgtaagaa gaggccacag aaggaagaat ttttatctga aaatcaagat
     7741 agaaatttac tcagttattt tgaagaatcg tggggagtcc tttgactcct cttacctttg
     7801 tcaaaaagcg aaaaaaatgc tacaataaag aaaataaaca aaatgaggta ttatcatgtc
     7861 caagattcta gtatttggtc accaaaatcc agactcagat gccatcggat catctgtagc
     7921 ttttgcctac cttgcaaaag aagcttacgg tttggatacg gaagctgttg cccttggaac
     7981 tccaaatgaa gaaacagcct ttgtcttgaa ctattttggt gtggaagcac cacgtgttat
     8041 cacttctgcc aaagcagagg gggcagagca agttatcttg actgaccaca atgaattcca
     8101 acaatctgta tcagatatcg ctgaagtaga agtttacggt gttgtagacc accaccgtgt
     8161 ggctaacttt gaaactgcaa gcccacttta catgcgtttg gagccagttg gatcagcgtc
     8221 ttcaatcgtt taccgtatgt tcaaagaaca tggtgtagct gtgcctaaag agattgcagg
     8281 tttgatgctt tcaggtttga tttcagatac ccttcttttg aaatcaccaa caacacaccc
     8341 aacagataaa atcattgctc ctgaattggc tgaattggct ggtgtgaact tggaagaata
     8401 tggtttggca atgttgaaag ctggtaccaa cttggctagc aaatctgctg aagaattgat
     8461 tgacatcgat gctaagactt ttgaactcaa cggaaataat gtccgtgttg cccaagtgaa
     8521 cacagttgac atcgctgaag ttttggaacg ccaagcagaa attgaagctg caatgcaagc
     8581 tgccaacgaa tcaaacggct actctgactt tgtcttgatg attacagata tcgtcaactc
     8641 aaactcagaa atcttggctc ttggtgccaa tatggacaag gtcgaagcgg ctttcaactt
     8701 caaacttgaa aacaatcatg ccttccttgc tggtgccgtt tcacgtaaga aacaagtggt
     8761 acctcaatta actgaaagct ttaatgcgta agattttggg tgtcagctca aaatcggaaa
     8821 gtctagtttg ccttatatcg aaaggagttt cggctccttt ttttctagga gtgaagtatg
     8881 ttagaaaatg gcgatttgat ttttgtgaga gatgggtcag acatgggaca ggccatccag
     8941 acttccacag gtaactatag ccatgttgcc atttatttgg atgggatgat ttatcatgct
     9001 agtggacagg ctggtgttgt ctgtcaagaa ccggcagact tctttgagtc caatcattta
     9061 tacgacctct atgtttaccc agaaatggat atccagtcgg tgaaggaaag agcttgcaga
     9121 catcttggag caccctacaa tgcttctttc tatccagatg cagctgcttt ctactgctcc
     9181 cagtatatag cagaaatcct acctattttt gaaactattc ctatgaaatt tggagatggg
     9241 gaacaggaga ttagtgattt ttggagggag tattacatag aactaggtct gcctgttcct
     9301 ctgaaccaag ctgggaccaa tcctagtcag ttggcagcat cgcctctgtt acaatgtaaa
     9361 gaaagggatc ttcatgattc agatttttaa tccatctcgt ttgacgagac agccattttt
     9421 ggagaattga tccgctatct ggaccagtat gaggatgtga ttctacggga aattaagtct
     9481 cagtttccag atgttgcagt tgataaactc atggaagagt atataaaggc aagcttgatt
     9541 ctacgtgaaa ataagcgcta ttaccttaat tttcctacgc ttgaatcact tgatagtctt
     9601 gaactggatc aagagatttt tgtcagagaa gctagtccgg tctatcaagc cttgttggag
     9661 cagagttttg agacggaatt gcgcaatcaa atcaatgcag ctattttagt tgaaaagacg
     9721 gactttgcgc gcattaaaat gaccctgtcc aattattttt acaaggtcaa acagcagtat
     9781 cctttgacag aaaaacagca ggagctctat gacattttag gagatgttaa tcctgagtat
     9841 gccctcaagt atatgacggc ttttttgttg aaatttctca aaaaagacca gcttatgcag
     9901 aaatgccgtg atatctttgt ggacagtttg gttgtcttag gctatattgt gcaaaatgaa
     9961 gatagaaagt atgagttggc tatcgatttt gataaggaga gattaacttt ctacttagcg
    10021 tgatttcttg tttctgagca cattgtttga ctttccttag tattcggtat aaactatatg
    10081 taaccggtaa cacatatcgg aataaactaa aggagacaat catatgtcac ttgaaaacaa
    10141 attggaacaa gcaacaggcg ctgtcaaaga aggttttggt aaagttactg gagacagcaa
    10201 gacagaactt gaaggagctg ttgaaaaaac agttgctaag gcaaaagacg ttgtagaaga
    10261 cgcaaaaggt gctgtagaag gtgccgttga aggtttgaaa aacgttttta ctaaataata
    10321 ggaaaaaatc aagggtttca ttttcccttg attttttcta ttc
//
LOCUS       DAVAAL010000059        10262 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVAAL010000059 DAVAAL010000000
VERSION     DAVAAL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.060"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            282..1724
                     /gene="gtfA"
                     /locus_tag="VQM60_001257"
     CDS             282..1724
                     /gene="gtfA"
                     /locus_tag="VQM60_001257"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET1081123.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSVFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNHSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            1862..3055
                     /locus_tag="VQM60_001258"
     CDS             1862..3055
                     /locus_tag="VQM60_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET1081124.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            3741..5720
                     /locus_tag="VQM60_001259"
     CDS             3741..5720
                     /locus_tag="VQM60_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET1081125.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            5787..7283
                     /locus_tag="VQM60_001260"
     CDS             5787..7283
                     /locus_tag="VQM60_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1081126.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            7283..8209
                     /locus_tag="VQM60_001261"
     CDS             7283..8209
                     /locus_tag="VQM60_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1081127.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINVVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            8218..9285
                     /locus_tag="VQM60_001262"
     CDS             8218..9285
                     /locus_tag="VQM60_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081128.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPCHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            9296..10222
                     /locus_tag="VQM60_001263"
     CDS             9296..10222
                     /locus_tag="VQM60_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081129.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         3030 a         1809 c         2154 g         3269 t
ORIGIN      
        1 aaactgacga agtcagtaac catacctacg gtaaggtgaa gctgacgtgg tttgaagaga
       61 ttttcgaaga gtataagaag agctcatgga atctatagtc tttgacacag aggaagagtt
      121 cagcttgttg ataattctat gtagaaaaca agaaagtagt actttgttta tcacagtcta
      181 tattagtacg agtttgatga taaatagaga tttaaaatga atttgattta cgaagccaga
      241 tcatatgaga cttggcttca attagaaaaa ggagagtaat gatgccgatt caaaataaaa
      301 ccatgttgat tacctattct gatagccttg gaaataatct taaagactta tatgataatt
      361 tggaagagca ttttggagat gctattggag gagttcacct tttaccattt ttcccatcaa
      421 caggtgatcg tggatttgcg ccagttgact acgacgaagt ggactcagtt tttggtgatt
      481 gggaggatgt taagcgttta ggtgagaaat attatcttat gtttgatttt atgattaatc
      541 atatttctcg tcaatccaag tattataagg actatcaaga aaaacatgaa gccagtgaat
      601 ttaaagctct ctttttaaac tgggataagt tttggccaga aaaccgtccg acacagtctg
      661 atgtagattt aatttacaag cgtaaggatc gtgcaccaaa gcaagagatt gtgtttgaag
      721 atggttcagt ggaacatttg tggaatacct ttggtgagga gcagattgat cttgatgtga
      781 ccaaagaagt aactatggaa tttatccgta agaccattca gcacttggca agtaatgggt
      841 gtgatttgat tcgtctagat gcctttgctt atgcagtgaa gaaattggat actaatgatt
      901 tctttgtgga accagatatt tgggatttat tggacaaagt tcgagatatc gctgctgagt
      961 atgggacaga gcttttacct gagattcatg aacactattc gattcagttt aaaatagcag
     1021 accatgatta ctatgtttat gattttgctc ttccaatggt gacactttat actctttaca
     1081 gttccagaac agagcgtttg gctaagtggt taaagatgag cccgatgaag caatttacga
     1141 cgctagatac ccatgatggg attggagtag tagatgtcaa ggatatcctg accgatgagg
     1201 agattgacta tgcttcaaat gaactctata aggttggagc caatgtcaaa cgtaagtact
     1261 ctagtgccga gtataacaac ttagatatct accaaatcaa ttcaacctac tattcagcgc
     1321 ttggagatga tgatgtcaag tattttctcg ctcgtctaat tcaagctttt gccccaggta
     1381 ttcctcaggt ttactatgtg ggtctattag caggcaagaa tgacttgaaa ttattagaag
     1441 aaactaaaga aggtcgaaat attaatcgtc attactatag caacgaggaa atagcaaaag
     1501 aagtgcaacg acctgttgtg aaggcccttc tcaatctatt ttctttccgt aaccattcag
     1561 aagcctttga tctagaaggg actactgaga tagagacacc aacagctcac agcattgtaa
     1621 tcaaacgtca aaataaagat aagtccgtaa cagcagtagt agaaattgat ttgcaaaatc
     1681 agacttatcg ggtaattgag aatggagttg aagtatcatt ttgaagcctt gatatggtgg
     1741 ataaaatagg gtttttattt tggaaaacgt ttcctttgtt ttcaaattgc taaaaaagtg
     1801 gtacaatata ggatagctta ctattatctg aatcagctga tttggagaga aaggattcat
     1861 tttgaaatca ataggcttta ttgaaaagct gaaggggttg tctagtaaag agctgatttt
     1921 attgggaatt atcctaagta tctttttacc cttttatctt tttgtagttg tactctgttt
     1981 atatattatc agtttgattt ttacaggaga catgaaaagt attcttcaga aaatggggga
     2041 gcatccgatg ctgcttcttt ttcttagcta tagtactgtt atatccattc ttgcacaaaa
     2101 ttggatgggt cttgtggctt cagtaggaat gtttctattt actattttct ttttgcacta
     2161 tcagtcgatt ttatcccata aattctttcg attgattttg cagttcgtct tgtttggtag
     2221 tgtcttgtca gctgcttttg ccagtttaga acatttccaa attgtgaaga aatttaacta
     2281 tgcttttctt tcacccaata tgcaggtgtg gcatcagaac cgggcagaag tgaccttctt
     2341 taatcctaat tattatggaa ttatttgttg tttctgtatt atgattgctt tctatctgtt
     2401 tacaacgacc aagttgaatt ggttgaaagt attctgtgtg attgcaggct ttgttaatct
     2461 ctttggtttg aactttactc aaaatcgaac tgcctttcct gctattatcg ctggagcaat
     2521 tatctatctc tttacgacta ttaaaaactg gaaggccttt tggcttagta ttggggtctt
     2581 cgcgattggt ttgagtttcc tcttttctag tgatttggga gttcgaatgg gtactttaga
     2641 ctcttctatg gaagaacgca tttctatctg ggatgctggg atggccttgt ttaagcaaaa
     2701 tcctttttgg ggtgaagggc cattgaccta tatgcactct tatcctcgga tacatgctcc
     2761 ttatcatgaa catgcccaca gtctttatat tgatacgatt ctgagttacg gaattgtggg
     2821 taccatttta ttagttttgt cttctgttgc tcctgttcgc ttgatgatgg atatgagtca
     2881 ggagtcgggg aaacgtccga ttatcggcct ttatctatct ttccttacag tggttgctgt
     2941 gcacggaatt tttgacttgg ctctcttctg gattcagtca ggctttattt tcttgctagt
     3001 tatgtgcagt attccattgg agcatcgaat gttggtatcg gacatgacgg attaagtttt
     3061 ataagtttga aatcttctac cttgggtagg agattttttt atacgatggg catgtcgtgc
     3121 atctgaggtg taagtcctct gtagggatcc actaccggtg aatacaatag cgctccccaa
     3181 gcctgactat cgtgaggtag cggattaaaa tggtctgggg atagatcgtt ttaagtctga
     3241 cgcaggaaat aagaattgtc agagaaagca atagtgaaat cgtaggctat gaatagaaac
     3301 gtgatagcga ggagcatata gtggataagg tagcgagaaa ttttaaagta taaaagatag
     3361 tcttaatcta tatatgtaaa tcacaaaagt aattgaattc ggaataaggt tggtgtgaaa
     3421 agacatagta aactgaatct tgaatactag aatgatactt ctacaatatt gttataattt
     3481 aatttgaatt ccaaaatcac attgttccgt ccgtatttca ttttactgta tgtcttccta
     3541 aactccaaag tttctgcgtc aaatttccta ttttcactgt agtctttgac acttcgaacg
     3601 acaatttgaa tcatatattc aaaaaaaatg aaaaatttgt gccaacaagc ctaaatattc
     3661 tgaaaatttg tttgattttt aatggataat gtgatataat ggttgtcaat ctataattca
     3721 cataaacaga ggagtagaaa atgaaaaaaa atcgtgtatt tgctacagca ggtcttgttt
     3781 tattagcagc aggtgtactt gcagcatgca gttcttcaaa atcatctgat tcatcagccc
     3841 ctaaagctta tggctatgtt tatacagcag acccagaaac cttggactac ctgatttcaa
     3901 gtaaaaatag tacaacagta gtgacttcaa atgggattga tggtttattc actaacgata
     3961 attacggtaa tcttgctcct gcagttgcag aggattggga agtctctaag gatggtttga
     4021 cctacactta taagattcgt aaaggggtta aatggtttac ctctgatgga gaagaatatg
     4081 cagaggtgac ggctaaagat ttcgtgaacg gtttaaaaca cgcagcagat aaaaaatcag
     4141 aagctatgta tttagctgaa aattcggtta aaggcttggc agattatcta tcaggaactt
     4201 caacagattt ttcaacagtt ggtgtcaagg cggttgatga ttatacgtta caatacactt
     4261 tgaaccagcc tgaaccgttc tggaactcta agttgaccta ttctattttc tggcctctga
     4321 atgaagaatt cgaaacatca aaaggaagcg attttgctaa accaacagat ccgacatcct
     4381 tgctttataa tggtccattc ttgttgaaag ggttgactgc aaaatcttct gtagagtttg
     4441 taaaaaatga gcaatattgg gataaagaaa atgtccacct agatactatt aatctagctt
     4501 actatgatgg atcagatcag gagtcgctag agcgtaactt cactagtgga gcttatagtt
     4561 atgcccgtct ttaccctacc agctccaact attctaaggt tgcagaagaa tacaaggaca
     4621 atatctatta cacacaatca ggctctggga ttgctggtct gggtgtgaat attgatcgcc
     4681 aaagttacaa ctatacttct aaaactacag attcagagaa agtagctact aagaaggcat
     4741 tgcttaacaa agatttccgt caagccttga attttgctct tgatcgctca gcttactcag
     4801 ctcaaatcaa tggtaaagat ggagcagctt tagcagttcg taatttattt gtaaaaccag
     4861 actttgtttc agctggtgag aagacctttg gtgatttagt cgctgctcaa cttcctgctt
     4921 atggtgatga gtggaaaggt gtgaatttag ctgatgggca ggatggttta ttcaatgctg
     4981 acaaggccaa ggcagagttt gcgaaagcta agaaagcttt agaagcagac ggcgttcagt
     5041 ttcctattca tctggacgtt ccagtagacc aagcatcaaa aaactacata tctcgtattc
     5101 agtcctttaa acaatctgta gaaacagttc ttggtgttga aaatgtcgtt gttgatattc
     5161 aacaaatgac aagtgatgaa ttccttaata ttacttacta tgctgccaat gcttcatctg
     5221 aggattggga tgtatcagga ggagtttcat gggggccaga ctatcaagac ccatctactt
     5281 acctggatat tttaaaaaca actagcagtg aaactacaaa aacatattta ggatttgata
     5341 atccaaatag cccttcagta gttcaagttg gtttgaaaga atacgataaa ttagttgatg
     5401 aagctgccag agagacaagc gacttgaatg tccgttatga aaaatatgca gcggctcaag
     5461 catggttgac agatagttca ctctttattc ctgctatggc ttcttctggt gcagcaccag
     5521 tgctttcacg aattgttcca tttactggag cttctgcgca aacaggctct aaggggtcag
     5581 atgtttactt caaatatttg aaattacaag ataaagtggt gactaaggaa gagtatgaaa
     5641 aagctcgtga aaaatggttg aaagaaaaag ctgaatcaaa tgagaaagct caaaaagaat
     5701 tggcaagtca tgtgaagtaa ataatttcta atagaacttt ctctccatga ggagaaggtt
     5761 cttttgggat ttttaaagga aataatatga aaaaatatat ttttatgcgt gttttgcggt
     5821 cattggtttc gattttctta gtaacgactt tgacctacac gattatctat accttggttc
     5881 ctcgaaaatt gattttcaag caggatccta actataataa aattgcgaca acggctgata
     5941 aacgtgataa ctatgaaaat actgtgtttg agcgtatggg ctacattgag tattacgata
     6001 ctaaagagtt gcaagaaaag gcaagtagca tggattcttc tgtaacagta gaagcaaatg
     6061 cgaccaataa agctatttat gaaaagtaca tcaatcaatt aggtcatggt tggactttgg
     6121 gagaatttac tgaaagtggt caattctatg ctactcgtga aattccaatt tttgaacgtg
     6181 tttttcactt ctatgctaac ttgattgaca ttgatcatac aaataaaatc caagaccctg
     6241 aaaatccaga cttgaaacgc taccttcgtt ttgaaaatga tccagctatc ggatggtcat
     6301 tggtcggttc aggaactaaa cataaatatc tcttgtactt taacagtcag ttcccatttg
     6361 ttcatcaaaa ctttgtgaac ttgaatttag gtgactctta cccaacctat gctaatacac
     6421 cagttcttca ggttattact caaggtcaag gacaaaccaa aactgcccaa gttcagttcc
     6481 caacaggtaa gaaaacgtct tctgtaaata tttactcaag aacctacaag tcacctagtc
     6541 aggctgactc tcgtgaagta gctagctatg ggaaagatga tccttataca gcgactgaaa
     6601 gtaattacca atatccatct atgattgtca gctctgctat tactggtttg attggtttgg
     6661 ttcttgccta tgctcttgcc gtgccacttg gttcagccat ggctcgtttc aagaacactt
     6721 ggattgatag cctctcaaca gggactttga ccttcttgct tgctcttcca acgattgcct
     6781 tggtttacat cgttcgattg attggatcat ctattgccct tccagattca ttccctatct
     6841 tgggagctgg agattggcgt tcttacgttt taccagcagt catccttggt ttgttgggtg
     6901 ctcctgttac agccatttgg attcgtcgtt acatgattga cttgcaatct caagactttg
     6961 ttcgtttcgc tcgtgcaaaa ggtttgtctg aaaaagaaat ttcaaacaaa cacatcttta
     7021 aaaatgccat ggttccgctg gtttcaggaa ttcctggtgc cattattggg gttatcggtg
     7081 gtgcaaccct tactgaaaca gtcttcgcct tcccaggtat gggtaaaatg ttgattgact
     7141 ctgtaaaagc atctaataac tctatggtcg ttggtcttgt cttcatcttt acatgtattt
     7201 ctatcttctc acgtcttttg ggagatattt ggatgactat tattgaccca cgtattaaat
     7261 tgactgagaa aggaggcaaa taatgtctac aatcgataaa gaaaaatttc agtttgtaaa
     7321 acgtgacgat tttgcctctg aaactattga tgcgccagca tattcttact ggaaatcagt
     7381 gtttaaacaa tttatgaaga aaaaatcaac tgtagtcatg ttgggaatct tggtagccat
     7441 cattttgata agtttcatct acccaatgtt ttctaagttt gatttcaatg atgtcagcaa
     7501 ggtaaacgac tttagtgttc gttatatcaa gccaaatgcg gagcattggt tcggtactga
     7561 cagtaacggt aaatcgctct ttgacggtgt ctggttcgga gctcgtaact ccatcctcat
     7621 ttctgtgatt gcgacagtga ttaacgtggt tatcggtgtt tttgtcggtg gtatttgggg
     7681 tatttcaaaa tcagttgacc gtgtcatgat ggaagtttac aacgtcatct caaacatccc
     7741 acctcttttg attgttattg tcttgactta ctcaatcgga gctggattct ggaatctgat
     7801 ttttgccatg agcgtaacaa catggattgg tattgccttc atgatccgtg tgcaaatctt
     7861 gcgctatcgt gacttggaat acaacttggc gtcacgtact ttgggaacac caaccttgaa
     7921 gattgttgcc aaaaatatca tgcctcaatt ggtatctgtt attgtgacaa ccatgactca
     7981 aatgcttcca agctttatct catacgaagc cttcttgtct ttcttcggtc ttggattacc
     8041 gattacagtg ccaagtttgg gtcgtttgat ttcggattat tcacaaaacg taacaaccaa
     8101 tgcttacttg ttctggattc cattgacaac ccttgtcttg gtatccttgt cccttttcgt
     8161 agttggtcaa aacttagcgg atgctagtga tccacgtaca catagatagg agtagaaatg
     8221 acaaaagaaa aaaatgtaat tttgactgct cgcgatattg tcgtggaatt tgacgttcgt
     8281 gacaaagtat tgacagccat tcgcggcgtt tcccttgaac tagtcgaagg agaagtatta
     8341 gccttggtag gtgagtcagg atcaggtaaa tctgttttga caaagacctt cacaggtatg
     8401 ctcgaagaaa atggtcgtat tgcccaaggt agtattgact accgtggtca ggacttgaca
     8461 gctttatctt ctcacaagga ttgggaacaa attcgtggtg ctaagattgc gactatcttc
     8521 caggacccaa tgactagttt ggaccccatt aaaacaattg gtagtcagat tacagaagtt
     8581 attgtaaaac accaaggaaa aacagctaaa gaagcgaaag aattggccat tgactacatg
     8641 aataaggttg gcattccaga cgcagataga cgttttaatg aatacccatt ccaatattct
     8701 ggaggaatgc gtcaacgtat cgttattgcg attgcccttg cctgccgacc tgatgtcttg
     8761 atctgtgatg agccaacaac tgccttggat gtaactattc aagctcagat tattgatttg
     8821 ctaaaatctt tacaaaacga gtatcatttc acaacaatct ttattaccca cgaccttggt
     8881 gtggtggcaa gtattgcgga taaggtagcg gttatgtatg caggagaaat cgttgagtat
     8941 ggaacggttg aggaagtctt ctatgaccct tgccatccat atacatggag tctcttgtct
     9001 agcttgcctc agcttgctga tgataaaggg gatctttact caatcccagg aacacctccg
     9061 tcactttata ctgacctgaa aggggatgct tttgccttgc gttctgacta cgcaatgcag
     9121 attgacttcg aacaaaaagc tcctcaattc tcagtatcag agacacattg ggctaaaact
     9181 tggcttcttc atgaggatgc tccaaaagta gaaaaaccag ctgtgattgc aaatctccat
     9241 gataagatcc gtgaaaaaat gggatttgcc catctggctg actaggagga aggaaatgtc
     9301 tgaaaaatta gtagaaatta aagatttaga aatttccttc ggtgaaggaa gtaagaagtt
     9361 tgtcgcggtt aaaaatgcta acttctttat caacaaggga gaaactttct cgcttgtagg
     9421 tgagtccggt agtgggaaaa caactattgg tcgtgctatc atcggtctaa atgatacaag
     9481 taatggagat atcatttttg atggtcaaaa gattaatggt aagaaatcgc gtgaacaagc
     9541 tgcggaattg attcgtcgaa tccagatgat tttccaagac cctgccgcaa gtttgaatga
     9601 acgtgcgact gttgattata ttatttctga aggtctttac aatcaccgtt tatttaagga
     9661 tgaagaagaa cgtaaagaga aagttcaaaa tattatccgt gaagtaggtc ttcttgctga
     9721 gcacttgact cgttaccctc atgaattctc aggcggtcaa cgtcaacgta tcggtattgc
     9781 ccgtgccttg gtcatgcaac cagactttgt tattgcagat gagccaattt cagccttgga
     9841 cgtttctgta cgtgcccaag tcttgaactt gctcaaaaaa ttccaaaaag agctcggcct
     9901 gacctatctc ttcatcgccc atgacttgtc ggttgttcgc tttatttcag atcgtatcgc
     9961 agttatttac aagggtgtta ttgtagaggt tgcagaaaca gaagaattgt ttaacaatcc
    10021 aattcaccca tatactcaag ccttgctttc agcggtacca atcccagatc caatcttgga
    10081 acgtaagaag gtcttgaagg tttacgatcc aagtcaacac gactatgaga ctgataagcc
    10141 atctatggta gaaatccgtc caggtcacta tgtttgggcg aaccaagccg aattggcacg
    10201 ttatcaaaaa ggactaaact agtaatggtt ttataatttc catgtcaatt tttatggaaa
    10261 tt
//
LOCUS       DAVAAL010000060        10133 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVAAL010000060 DAVAAL010000000
VERSION     DAVAAL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.061"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            119..331
                     /locus_tag="VQM60_001264"
     CDS             119..331
                     /locus_tag="VQM60_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081130.1"
                     /translation="MMIVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQTK"
     gene            747..1109
                     /locus_tag="VQM60_001265"
     CDS             747..1109
                     /locus_tag="VQM60_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET1081131.1"
                     /translation="MELVLPNNYVVLEQEEMIYLDGGGVGCNWWNKRDNIAAAAVSGG
                     MSVYQTYAIKKLIANRGMMSRVVRSAITRYIGGVSMATITNALNIAATVAEVFSLGGA
                     IAYGLDIVDGKFDGYLWA"
     gene            1090..1335
                     /locus_tag="VQM60_001266"
     CDS             1090..1335
                     /locus_tag="VQM60_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081132.1"
                     /translation="MDIYGRKTIAMKHFFAGIGEIHFLLFLLYLSMSTSPIIGLVIGY
                     TKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
     gene            1492..2550
                     /locus_tag="VQM60_001267"
     CDS             1492..2550
                     /locus_tag="VQM60_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081133.1"
                     /translation="MKDDQKYLLAGFYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIGTVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLIQNQSKVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALRDLSKQYLNKNISELQDGSFEDGIQDAVASKQIVVINEFIACLNSRCVPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            2866..3843
                     /locus_tag="VQM60_001268"
     CDS             2866..3843
                     /locus_tag="VQM60_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081134.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGVAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKDCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLNKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            4220..4594
                     /locus_tag="VQM60_001269"
     CDS             4220..4594
                     /locus_tag="VQM60_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET1081135.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            4802..5704
                     /locus_tag="VQM60_001270"
     CDS             4802..5704
                     /locus_tag="VQM60_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081136.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFVIIMLTTIFFWNIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTKTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIHLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            5760..6113
                     /locus_tag="VQM60_001271"
     CDS             5760..6113
                     /locus_tag="VQM60_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET1081137.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            6110..6838
                     /locus_tag="VQM60_001272"
     CDS             6110..6838
                     /locus_tag="VQM60_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081138.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLVINSILLISFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGKDFDTKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKAKEYELFKNSK"
     gene            7973..8212
                     /locus_tag="VQM60_001273"
     CDS             7973..8212
                     /locus_tag="VQM60_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081139.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            8764..>10133
                     /locus_tag="VQM60_001274"
     CDS             8764..>10133
                     /locus_tag="VQM60_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
                     /protein_id="HET1081140.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTVVRAEESPVASQSKAEK
                     DYDAAVKKSEAAKKHYEEAKKKAEDAQKKYDEDQKKTEAKAEKERKASEKIAEATKEV
                     QQAYLAYLQASNESQRKEADKKIKEATQRKDEAEAAFATIRTTIVVPEPSELAETKKK
                     AEEAKAEEKVAKRKYDYATLKLALAKKEVEAKELEIEKLQYEISTLEQEVATAQHQVD
                     NLKKLLAGADPDDGTEVIEAKLKKGEAELNAKQAELAKKQTELEKLLDSLDPEGKTQD
                     ELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADSEDDTAALQNKLATKKAE
                     LEKTQKELDAALNELGPDGDEEETPAPAPQPEQPAPAPKPEQPAPAPKPEQPAPAPKP
                     EQPAPAPKPEQPAKPEKPAEEPTQPEKPATPKTGWKQENGMWYFYNTDGSMATGWLQN
                     NGSWYYL"
BASE COUNT         3473 a         1361 c         1864 g         3435 t
ORIGIN      
        1 agtttgggag atatttttat ttattttagg tgtgttgtta attgtaattt cattactaag
       61 tcccccaaaa attaaatttt ggaaatagag ttagtgagaa ggataggata atagtattat
      121 gatgattgtt ttaatttcag ttattgttgc attcctgtat cataaaatta cagagtatgt
      181 caacaacagg aaaattgata ttgttatttt tctatcattg ctgatttctc attttatttg
      241 gaattactat tttttaccaa ttgaaatatt gatagcaatt agtggagcaa atttattatt
      301 aagtttttta aaaaaattac agactaaata atattaatct gtgtaataca gtagctcggt
      361 cattagattc tattgaagat ataaattaaa aacgaggttg ggaaaaaact caatttgaaa
      421 atgagattga gtaaatcttt ctatctatat aacgtatatc atcaatattt ttgaacacct
      481 gataatatgc atttttctta ttttaaagat tttttgcctt tcttcatttc ttgaatgaat
      541 cggagagatg actttattta ggtgtttgac attactatag ataggattta gcagtactaa
      601 tatgaactca tattataggt gcagacttaa attttggaag caagctaaag gatatcttat
      661 ctaggtaact aagtgctata aacaaagtca acgatcactt gttaggaaac tatcctaact
      721 atgcttaata aaaaaggaga aaataaatgg aactcgtatt accaaataat tatgttgttc
      781 ttgagcaaga agagatgata tatcttgatg ggggtggtgt tggttgtaac tggtggaaca
      841 aacgtgataa tattgctgca gctgcagttt ctggagggat gtcagtatac cagacatatg
      901 ccattaaaaa gttaattgct aatcgtggta tgatgagccg agtcgttaga agtgctataa
      961 caagatatat tggtggagtt agtatggcaa ctattactaa tgctcttaat attgcggcta
     1021 ctgttgctga agtcttctca ttaggaggag ccattgcata tggtttagat atcgtagacg
     1081 gtaaatttga tggatattta tgggcgtaaa actatagcta tgaaacattt ttttgcagga
     1141 attggtgaaa ttcatttttt gttattttta ttatatctaa gcatgtctac cagtccaatt
     1201 attggactag tgattggata tactaaaata aatctaataa attctattat tatagctctt
     1261 ggtatgatat tggcattaat tttggtatta tcaagagtgt atagaaaata tttttataga
     1321 gacgatgatg actaaagttt ttatcaataa tttgggctcc ttgtcaactg tagtgggttg
     1381 aataaaagct aacgtctggg gaagggttta aagctttgct ttatcaaata ttgacttaca
     1441 gagttgctct attgtattga tgtattctta gtaaataata tggtgttttg aatgaaagat
     1501 gatcagaaat atttattagc gggattttat tcgcttctag ttgcaatatt ttattttcca
     1561 ttgattgatt ccaaggggat ttttgtaagt atattgatgg cggttttact tttatattta
     1621 atatacttca ttggaacagt cattcatatt gtgataatta agtttataag aaaaaaatct
     1681 tttaagtatt tggttctata tccatttact tatgatggga gctggagatt tcaacctata
     1741 aacttactct actttccaga aatggtgaga gatgtcattc cgattaattt agttcaagaa
     1801 tattgccaag gacaacctta cggactgtta aaaaagatgc tgaaaagaat tcgattgtca
     1861 cgagagatat ctttactcct ggctactatt atcgtctatt tttttacgca tagaattcta
     1921 cctctttcag tatttacgtt catattttct tacatattgc tatttgcaca atcttatttg
     1981 ggtggtaata ccgtttggat tggaaataga agattaataa tagatgatga atttgaaaaa
     2041 atcttgcttt caaaaagtta tataaaagaa atatcatctg cacgatactc ggaatacttg
     2101 acatgtgaat acaaaaacct tacaccaatc atattgttag ctatttttga aaatttgctt
     2161 gactcctact tgattcagaa tcagagtaag gttgacttag atatttttta taaagtttta
     2221 cctttgcttt ataaagaaaa atatactatg ggattcaatt actttgtaag tttaaattat
     2281 ttactatata aggttggatt tttgggaatc atttacgata atgaagcatt aagagatttg
     2341 tcaaaacaat atttaaacaa aaatatttcg gaattacaag atggttcttt cgaggacggt
     2401 atacaagatg ctgttgcctc taaacaaata gtagttataa atgaatttat agcttgttta
     2461 aattccagat gcgtgccatc ccaatatgat agattttttt ataaagatag accttatatt
     2521 ttttccagaa agagtcctat taaggggtga aaatttgtta catttggatt aacaagtgtc
     2581 ctagggatta gaaataactc tagtatagct agatattttt gagtaactag aaatatcatt
     2641 aggctactca tatgattgcc agaaaagatg gctgatgaat cttatttata ctgttgcagt
     2701 agggcattgt ctaggataaa taagctatct tgatggggga gattggaata attttagtag
     2761 aaacattaaa ggattatgga caagtagcgc aggattaggt gatgcaatgt gctaagttgg
     2821 cttggtgtta gcagtagtat tatttaacaa tggaaagtag actaaatgaa aaataaacgt
     2881 tatttttttg acacaatttt aataatttta ctgcttataa gcacgatatt ttgtgtcagc
     2941 ccagtattta taaaattgga catcttagga accccctctc atgccatctt aacctttgta
     3001 ttggcgatac cgttattcta tattttgtct caatgcttac atactttgct attactagtt
     3061 tcctcaatct tttgtaaatt aagaccaatt tatttttatt ttatatttgt gatcattata
     3121 ggagctagga aatactatcg aattctcttt caccaactaa tgggatttag tccaggagta
     3181 gcagtgtttt ataaggagag tcaaacaact aagaatctgt ttaaatttta ctacttcttg
     3241 tactttacaa cactaatttc atactatttt ttctttactt ttgtgtatga caaaccgttg
     3301 cttttgcctc ttattccatt ttctattatc atagcattag tacaaaaact ttatagaatt
     3361 gagaatcaac aattatttct gttaaaatca aaggtactaa caatactgga atctaagaag
     3421 gattgtgagt ttaatcttca ggattatcat gaaatttgga aattgcaatc aaaatctgag
     3481 ttgccatgcg tggctttatc ttatatcagt ttgatcaagc catatctttc agagagcgtg
     3541 cgtgaacaaa ttgatttatt agaagttaag cgatttaaaa aaataaatca tccaatatca
     3601 ctttatggta tgttggatgt tataaaatta aatctatatt tacggcatta taatgaaaaa
     3661 aataaatatg aatcaatgtt aaataaaatt ttagaggtaa gaccagactt tgttttaatc
     3721 gagcagaata ttgatgatag tttaaatagc tcgcaaccat tatctttatc tttagccatt
     3781 tcagaaatcc aacttttgct tgaagtgtat atggggatca aacatgtttc aattaggaga
     3841 taaaaccaga ataatatttg ccattatagg ggcaagttgc atattagtca gtatgtatct
     3901 tatacctctc attaacacgg ataataaatt ttgggaaata cttttattta taataggtgt
     3961 attgctaatt ggaattgagt tactaagtca aaaaaaataa attttgaaat agtattagtg
     4021 agttcgcaat catttattac tagtggttta gaactgttaa gtttcaaaca ataattgatt
     4081 ttaaacctta ggtaacctat aaaaacaaat aaggtagaga aaggatatct tatctaggta
     4141 gctaagtgat aaaaacacag tcgtctatca cttgttagga aactatccta actatgctta
     4201 ataaaaaagg agaaaataaa tggaactcgt attaccaaat aattatgttg ttcttgagca
     4261 agaagagatg atgtatcttg atgggggatt ttctattccg agatggcctg ttgcaacagc
     4321 cattaatata gcttttaatg gtgttttagg tggaggagca atcagtctag ttagaaatta
     4381 tattcgtaat tatggtttgc ggcgagttac aagcgcaatt gctggagcag ctgcaagata
     4441 tgttggggta cgagttgcaa atagagtggc aggatttgca ctgtctgcta ttaatggatt
     4501 tgcagcttgg atgtcaattg gcgatgctat tacaacaatc tgggccaaca atgatgtaaa
     4561 taggagagac ccaaatttaa acgccttgtg gtaactagat atgggtatta aaatggaaaa
     4621 aatttttgtt attatttttt ttgtatgttt atttatatca tccataactt ttttagccta
     4681 tgattttgtt agcgaagaaa taaaaaagtt gattatttgg atgaatgtcg tttttttgat
     4741 tttaatcata gcaatgataa tttatccaaa attaagaaaa tgataaggac actgtaaaat
     4801 tatgatacgt aaactaatca tttttttatt aatgctccct ctttttgtct tgtggctaga
     4861 gttgcatgtc ctagtgaata acttactatt gaatctagaa tctccccttg attttgtgat
     4921 tagtatgagt cttgctttct gtagtttgat tttatcaaaa atagttttag acatactata
     4981 cgcattgaaa gatttgtaca agaaagaagc tcttattacg atttttcctt ttatttttat
     5041 aggcaggaag aaagtaaatg tgaggttctc gccctatttt tcattccatc gtaaaagttt
     5101 gtcgcctgat gatttaagat ctaggattat atggagtttt attcttgaaa ttgctattat
     5161 cctagtattt attttaaaaa ttccttttgt catcattatg cttactacaa tatttttttg
     5221 gaatataatg gatatcaatc atctcgtttt taataagacg gaatttcttt tcaatcaaaa
     5281 taaatgggaa aaagaagatt cgtttgaaag cgatttgacg aaaacgttaa aggataaaat
     5341 acaaaaatca gagctaagtt attctgactt aatgtctctt cagctgtatg atgcgatgaa
     5401 tcaatctacc tttttaacgg atagtgaact ctttgaggat attctgagta aaattgaaga
     5461 ttctcataac acccttctgt gtacaggtct tgtcgagtta ctgctgtatg agatttctat
     5521 tagtaacaat aacaactggc aagaaaaggt tgataaaatt aggatacacc tcatcagaat
     5581 caatcaatta gattttttct attatacaag ttggttaagg caaaattttg atttctgtat
     5641 gaatagagag tatcataaaa tgaaatctcg gaaattatta ctgagtaata aaaagatagt
     5701 ttaagttcgt cctatttgta aaacaaatga aacaatttca actaaggagg agaaaacaaa
     5761 tggaacttgt attaccaaat aattatgttg tgattgatga agaagaaatg atgtatcttg
     5821 atgggggagc ttatttaagc aagcgtgctt gtcaaggaat ttgcgcagct ttagctatga
     5881 gttcaggaac ttttatagca ttagctggag ctgcagtttt aaccaaaaaa ctaataaact
     5941 atattaaggt tggaggtctt ggaggctggc ttattggtgc agcagcaggt gtattggcta
     6001 cagcagcagg gaaaatagct tactgtattg gatatggtgt tcttaataga ggttgtgata
     6061 ttaacgggaa cccttatcct tgggatggat tcatatctgc aacagtaaga tgagtaatgt
     6121 agataagatt cgaaaaatac acatcatcgt gtgttgggtg tatatatttt tatcattcag
     6181 ggcgattata aatgatacgg agtatttttt gcttattttt ttagctttta tctactcaat
     6241 agtatcacta cctctatatt ctgtgaaaaa taaaatagta tctatctgtc tagttataaa
     6301 ttcgattctg ttaatcagtt tccccatttt aatcaataaa tttttcccag aaagtttttt
     6361 gacttatacc gtattaataa gtgtttttat cctagagtta gcaatctttc atttaattgg
     6421 gaaagatttt gatactaaat tgactaacga atataagaaa attagtcagt tcagaagcaa
     6481 ggtgtctcaa tctccttgga taaaatattt agagatttct agttttatat taactatatt
     6541 tccatccatt ctttatggta cagttgataa tcatgtactg actcttatct ttttgataaa
     6601 aatttgtgta gataccacga taaaattttt attcatcaga ttatttgaca caagtacttt
     6661 aatgaagagg agaatatttt ttcttttcgc attggatgtt atagcttact tatttttagg
     6721 atatctttta gtgattcaaa aagcaggcta tttgttttcg gttttacttc ttttttctaa
     6781 tttttcagtt ccatttatca aagcaaagga atacgaatta tttaaaaata gcaagtaata
     6841 gaaacatagg ttaaatctta gaacggctta aaatcagata tgataaaggg aaatagtctt
     6901 atctaggtaa ctaagtgcta caaacacagt cgaggatcac ttgttaggat tacattttaa
     6961 tcatgcttaa taagaatgga gaattcctat ggggattgta ttactctgaa ctttcgaata
     7021 ccttagttct cgtgattttg agctattcta ggtttagaat ccttcagaat gtaaaatttt
     7081 aagcagtcaa agacttagaa tagcctgaaa acatttattt tatagcaatt ctaaacttag
     7141 aattgctcat atatatgtat ttgagctgct cgatagttag gacagctcta ttctgagaaa
     7201 tctgagctat caatgcttca gaatagtatt tcgccaatgg tttttattga agaggtgatt
     7261 gttttatgat agtctaaaat aaaactgatt taatggagta aatggagaat cgttacatga
     7321 aacatttttt tgcaggaatt ggtgagataa aacttgttgg ctacttattg tatatatgtg
     7381 taggactagc ccctcttttt catatatatg tcataggttc ggaactgagt tttgtgaaaa
     7441 tagtgttatc tattttggga gttatatttg taagtatgtt aactattgct cgaatttatc
     7501 ggacttttct ataaagaata gtttggactt gaactgtatc taaaaacagg ggttggtcga
     7561 ttatcagcct ctttcttgtc gttgaaaaga gtgagatata ttgacttttg aaaaaaagat
     7621 gtcataattg agttgggata aacctaaaag taatatagta gattgaatct ggaatagtcc
     7681 gttatgactg ttaaaatatt tctagaaatt aatttgactt tcctaatcga tttgttcaca
     7741 tcttgtttca atctactata ctttctcgtt aaaccgttcg gcaattcatg gtgacatgga
     7801 cacatagtag taagcacact atgtggcttg gcagagctaa aaactgttcc cttgcgataa
     7861 gcctaataag cacaacatag ggagttagag aagccgactc taatcaacca ctttgggcag
     7921 tagtgagaac tgccccgtgc ttttttattt tgagaaaata tggagtttgt cgttgaaatt
     7981 acttgattgt attttggatt atcaagaaag attcaatgga aaaacatgtc aagtatcaac
     8041 gaattataag tatttagaga ttttcaaagt gaatttttgc ttgactgatt tacatcattt
     8101 atttggctta cacaaaatca cacgagatta tgctagtcaa acaaaacctg ctattcaagc
     8161 tggtgttttt attttagaag attttagaaa tatcctatgt acaatgatgt aatcgaaagg
     8221 atatctttat gtgagtttat cggcgatatc ttctatatct aagctaacaa gttgttgcat
     8281 cgtagctaag gatttatcta aaaatactat gaaattggac gtcatatttt ttgaggataa
     8341 aaataaaagt tccgcagttt taggtttacg aagagacaaa agcggagtat ttaaaccagt
     8401 tactctacat tttacaagcg ctaagaaata tgctaaagtt cgtaaaacag atgtgaaaga
     8461 aatgaaatgg ctataaagat tgtccgcagg cttaagcttg cgttttttgt tgtttaacga
     8521 aattaaaaaa taagctctct catcggaaat gtttttatag gaatgaagga agatgatgct
     8581 atgtttaaaa agagaatcga aaggggcaaa agtagtgtct ttatcttttt ggagtagaag
     8641 cttatgatat agaaatttgt aataaaaatg taatataaaa catttgacaa atatttacgg
     8701 ggggggggta tacttaatat aagtataatc taaaaatgat tatcagaaaa gaggtaaatt
     8761 tagatgaata agaaaaaaat gattttaaca agtctagcca gcgtcgctat cttaggggct
     8821 ggttttgtta cgtctcagcc tactgttgta agagcagaag aatctcccgt agccagtcag
     8881 tctaaagctg agaaagacta tgatgcagca gtgaaaaaat ctgaagctgc taagaaacat
     8941 tacgaagaag ctaaaaagaa agcagaagac gctcagaaaa aatatgatga ggatcagaag
     9001 aaaactgagg caaaagcgga aaaagaaaga aaagcttctg aaaagatagc tgaggcaaca
     9061 aaagaagttc aacaagcgta cctagcttat ctacaagcta gcaacgaaag tcagagaaaa
     9121 gaggcagata agaagataaa agaagctacg caacgcaaag atgaggcgga agctgcattt
     9181 gctactattc gaacaacaat tgtagttcct gaaccaagtg agttagctga gactaagaaa
     9241 aaagcagaag aagctaaagc agaagaaaaa gtagctaaga gaaaatatga ttatgcaact
     9301 ctaaagctag cactagcgaa gaaagaagta gaggctaagg aacttgaaat tgaaaaactt
     9361 caatatgaaa tttctacttt ggaacaagaa gttgctactg ctcaacatca agtagataat
     9421 ttgaaaaaac ttcttgctgg tgcggatcct gatgatggca cagaagttat agaagctaaa
     9481 ttaaaaaaag gagaagctga gctaaacgct aaacaagctg agttagcaaa aaaacaaaca
     9541 gaacttgaaa aacttcttga cagccttgat cctgaaggta agactcagga tgaattagat
     9601 aaagaagcag aagaagctga gttggataaa aaagctgatg aacttcaaaa taaagttgct
     9661 gatttagaaa aagaaattag taaccttgaa atattacttg gaggggctga ttctgaagat
     9721 gatactgctg ctcttcaaaa taaattagct actaaaaaag ctgaattgga aaaaactcaa
     9781 aaagaattag atgcagctct taatgagtta ggccctgatg gagatgaaga agaaactcca
     9841 gcgccggctc ctcaaccaga gcaaccagct cctgcaccaa aaccagagca accagctcca
     9901 gctccaaaac cagagcaacc agctccagct ccaaaaccag agcaaccagc tccagctcca
     9961 aaaccagagc aaccagctaa gccggagaaa ccagctgaag agcctactca accagaaaaa
    10021 ccagccactc caaaaacagg ctggaaacaa gaaaacggta tgtggtattt ctacaatact
    10081 gatggttcaa tggcaacagg ttggctccaa aacaacggtt catggtacta cct
//
LOCUS       DAVAAL010000061         9918 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVAAL010000061 DAVAAL010000000
VERSION     DAVAAL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.062"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(26..1729)
                     /gene="ilvD"
                     /locus_tag="VQM60_001275"
     CDS             complement(26..1729)
                     /gene="ilvD"
                     /locus_tag="VQM60_001275"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET1081141.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(1962..2894)
                     /locus_tag="VQM60_001276"
     CDS             complement(1962..2894)
                     /locus_tag="VQM60_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET1081142.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSSSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(2891..3748)
                     /locus_tag="VQM60_001277"
     CDS             complement(2891..3748)
                     /locus_tag="VQM60_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET1081143.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(3752..5098)
                     /locus_tag="VQM60_001278"
     CDS             complement(3752..5098)
                     /locus_tag="VQM60_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET1081144.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(5111..5395)
                     /locus_tag="VQM60_001279"
     CDS             complement(5111..5395)
                     /locus_tag="VQM60_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET1081145.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(5399..7429)
                     /locus_tag="VQM60_001280"
     CDS             complement(5399..7429)
                     /locus_tag="VQM60_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET1081146.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            7620..8627
                     /locus_tag="VQM60_001281"
     CDS             7620..8627
                     /locus_tag="VQM60_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET1081147.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            8630..8830
                     /locus_tag="VQM60_001282"
     CDS             8630..8830
                     /locus_tag="VQM60_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET1081148.1"
                     /translation="MSEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            8982..9164
                     /gene="rpmF"
                     /locus_tag="VQM60_001283"
     CDS             8982..9164
                     /gene="rpmF"
                     /locus_tag="VQM60_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET1081149.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            9180..9329
                     /gene="rpmG"
                     /locus_tag="VQM60_001284"
     CDS             9180..9329
                     /gene="rpmG"
                     /locus_tag="VQM60_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET1081150.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(9809..>9918)
                     /locus_tag="VQM60_001285"
     CDS             complement(9809..>9918)
                     /locus_tag="VQM60_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET1081151.1"
                     /translation="TVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         3234 a         2089 c         1595 g         3000 t
ORIGIN      
        1 tttgaagaga ttttcgagta tgagtttatt ttttacctga cttgtccata ttccagaagt
       61 ctgtcacggc tccgcgtgaa gcagatgata cgatgtgggc atatttaccg aggacaccac
      121 ggctgtaaag tggtggcaag gttgtttctg ccttgcgttt ttcaagttct tcttcggata
      181 cggccatgga aatttctttg gtatcttggt caaccgtaac gatatcgccg gtacggagat
      241 aggcaattgg tccaccatcc tgagcttcag gagcgatatg tccaacaaca agaccataag
      301 taccaccaga gaaacgtccg tccgtcaaga gggccacctt atctccctga cctttaccaa
      361 caatcattga agaaagtgac agcatctcag gcataccagg accaccttta ggtccaacaa
      421 aacgaacaac gactacatcg ccatcaacga tttcatctgt cagaacggcc tgaatcgcat
      481 cttcttctga gtcaaagacc ttagctggcc caacgtgacg acgcacttta acacctgata
      541 ccttggcaac tgcaccgtca ggagcaaggt tcccgttcaa gatgataagc ggaccatccg
      601 cacgttttgg attttcaagt ggcatgataa ctttttggcc tggagtcaag tctgcaaagt
      661 cagccaagtt ttcagctaca gtcttaccag tacatgtgat gcgatctccg tgaaggaaac
      721 catttgccaa caagtacttc ataaccgcag ggacaccacc gacttcgtag aggtcttgga
      781 agacatactg accagatggt ttcaagtcgg ccaagtgagg cacacgttct tgaatcgtat
      841 tgaagtcctc aagtgacaag tcaacatttg cggcatgggc aatggcgagc aagtgaagag
      901 tggcgtttgt agaaccaccg agagccatcg ttacagtaat agcatcttca aaggcttcac
      961 gagtcaagat atctgatggt ttgagaccaa gttccaacat cttaacaaca gcacgtcctg
     1021 ctgcttcgat atcttctttc ttatcagctg attcagctgg gtgagaggat gaccctggca
     1081 aactcatccc tagaacttcg atagcagttg ccatggtatt agcagtatac ataccaccac
     1141 aaccaccagg gccagggcag gcattacatt caagacgttt cacgtcctca gctgtcatgt
     1201 caccgtggtt ccattttccg ataccttcaa agacagaaac caagtcgata tctttaccat
     1261 caagatttcc cggtgcaata gttccaccat aggcgaaaat agctgggata tccatattag
     1321 caatagcaat catagatcca ggcatgttct tgtcacagcc accgatagcg acgaaggcat
     1381 cgacgttgtg accactcata gccgcctcga tggagtccgc gatgatgtca cgagatgtta
     1441 gagagaaacg cataccaggc gttcccatag cgatcccgtc tgctacggta atggttccaa
     1501 actgtacagg ccaagcgcct gcagatttga caccttcttt agccagtttc ccgaaatcat
     1561 gcaagtgaat gttacatggt gtattttccg cccaagtcga aatcactcct acaatcgatg
     1621 tttcaaagtc cttatctgtc ataccagtcg cacgaagcat agcacggtta ggtgatttaa
     1681 ccatgctgtc ataaatgcta ctgcggtgac gtttatctaa ttcagtcatc ttatccctcc
     1741 catttcagtt tttactatta tagcacaatt taaaggcaat gaacagaata aaattcttga
     1801 attttcagaa aattctatac gcatgtgaaa tatttaaaat taaaaacaac aaagcggatt
     1861 agtgcacttt ctgatgacca gaatatgctt tttaatccgc tttttttaaa taacgtaatg
     1921 taatttttac agaaattctt tcagataagt gtatttaaca tctatcttgc attataaatt
     1981 tctagaacct tctcttttat attcgattca ctcaaaccat actcattaag aagataatcc
     2041 attttcccta cttgaccaaa tctttcttga acacccatcc gatgaatttt tgttattcca
     2101 tcatcagaga ataattcaca taaagaactg ccaattccac ctatctgatt gtggttttct
     2161 acagtaaata tagtttttcc acttaacatt gtttttatct gttctggtat cggtttgatt
     2221 ctaaataaat ctatcacacc tactgaataa cctaatttag acagttcatc tgcaactcga
     2281 atacttggag caaccattat accagaagca acgattacaa gatcttcacc atgtcttaac
     2341 tcaatgtagc ctttagaaaa atcttctcca ccttgataca caggaactgg agcttttcta
     2401 attgttcgaa tatattttag tccttttaag tctaatgtct ggttcaatat ttcacgaaat
     2461 tggatatcat cagttgcttc aaaaatgatc gatttaggaa ttaaacgtaa caatccaatt
     2521 tcttcaaatg gcatatgtgt cccaccattc atttctgcag ttactcctgc atctgatcca
     2581 atcacagtgg catccaattg tgcgtatcca agagaaataa ataattgatc aaataccctt
     2641 cgtgaagaaa aaggaccaaa tgtatgaaga taaggtctaa acccctgaat agacaagcct
     2701 gctgcaaggc cgaccatttc tgcttccata atcccaacat tcacataacg gtctccaaag
     2761 tccttttcaa gattatgagt agccatcgaa cttgacaaat cggcttctaa gactactata
     2821 tcagaagaac tttgattagc ctctaaaagg aagtctctat atacatgccg taattctttc
     2881 gtacttctca tcattctgtt tcctccaatt cctgacttaa tctttctaca actgaagtta
     2941 acatttgttt ctcctctaca gtagggcgaa gatgatgatt ggatttcatt tcttccagct
     3001 cttgaacccc ttgaccttta atagtatcta atacaatgca cttaggtgat gaattatttg
     3061 actgttttaa ttggacaatc ccttcataaa tttctctaat atctgaaccc ttgaccctaa
     3121 tggattcaaa tccaaatgct gaaaattttt ctacgaaatc acctggatta caaatatcct
     3181 ttgtaaaacc atctaattgt tttttgttat catcaacaaa tacaattaag ttggataact
     3241 gttgatgaga agcaaactgt atagcctccc aacattgtcc ttcatttaac tcaccatctc
     3301 caacaatagc ataagtataa aagggactct ttcttattct ctgaccatat gcaagtccag
     3361 ttgcaacact aattccttgt cctaaagagc ccgttgtcat atctatgcct ggcgttagat
     3421 ttctatcagg atgagacggt aatttggttc catttgtatt taaagaatat aagaattctt
     3481 tgtcaaagaa accattcaaa tagagtgtac tgtatagagc tggtcctcca tgaccttttg
     3541 ataatatgaa ataatctcta tctcgtgctg caaatatttc tggagtcatt ggcattattt
     3601 caccataaag caccgctaaa acttctacga tagacagact tcctccgtaa tgtccgaatc
     3661 caagatgatt caatgttcta agagtattta atcggatgtt agtcgcaaat tttcttaact
     3721 catcttctct atttttactt aaaatcatcc cttattcctc ctttgcagat ggctttttaa
     3781 taaaggatac tccaaacata actgctagaa taagaacaag accaatcaca atgcctgctt
     3841 gtgagccaaa ttgatttaac attcctaaaa taattcctga tagaccaaaa tctgcatctg
     3901 agaaagttga tccttggaaa ccaagtcctc ccaaaactgg cattaaaaag actggaagaa
     3961 aactgattaa aataccttgt aaaaatgctc caatagtggc tccacgaaca ccaccagatg
     4021 cattcccaat gacacctgca gtcgctccac agaagaaatg aggcacaaca cctggtaaga
     4081 taacaaccgt tcctgaagca atcataattg ccatacttac taaaccacca ataaaactag
     4141 agataaatcc aattagaact gcattgggtg cataagtata aacaatcgga caatccaaag
     4201 caggttttga attaggtaca agacgctctg aaataccttt aaaggctgga acaatttcac
     4261 ccaaaataag gcgaacacct gctaaaataa caaatacccc tgctgcaaat tgacctgcta
     4321 attgtaaagc ataaactaga ccacttgtac cactactgat ttctttttct atatattctg
     4381 accctgcaaa gatagctaca ataatgtaaa taactgccat agataaagtg atactaacag
     4441 tactatcacg taaaaaagct aaactctttg gaaatttaat gtcctctgtt gattttgatt
     4501 tgtcaccgat aaggctacca gtaaaaccac tcaaccaata tcccaaagaa ctgaaatgac
     4561 ctaaagccac cttgtcattt ccagttaatt gaaccatata tttttgcaca aatgctgggg
     4621 aaatactcat aataataccg agtgctaatc ctcctagtaa gatgagaggc aagctagtaa
     4681 agccagcaac tgataaaatg accgcaatca tacatgccat atatagagtg tggtgccctg
     4741 ttaaaaagat atatttaaat cgagtaaaac gagcgattaa gatattgaac accatgcctg
     4801 caaacataat cagtgcagta gctgagccat atgttgttaa agctacagct acaattgctt
     4861 cattattcgg cacaacgcca gataaatgaa aagcatgctc aaacatggta ccaaatggat
     4921 ttaaagaatt ttgtacaatt cctgcaccac cagatacaac taagaaacca acaaaggtct
     4981 taattccacc tttaataata tcaggtaatt tcttcttctg aagaactaat cctaaaattg
     5041 caattaaagc tactaaaata gctggtgtac taacaatatc caatatgaac ttcatcataa
     5101 cgctagcctc ctatataagt cctttttctt cacaaagttt agtaattaat tctcgtagtt
     5161 catccatatc aataatacta tttaagatac gaacatctcc aagatgacta gctgaatcag
     5221 ctagatcacg accaacaatc caaatatcag ctgcatttgg atctgctcca cctaaatcat
     5281 aatgttcaac ttctacatcc gaaacattca aatcactcaa tacagattca atattcatct
     5341 gtaccataaa acttgaacct aatcctgaac cacaagctgt accaattttt aacattatct
     5401 aatcctcctg tttaattatc attttaatgt catcatagtt ttttgatgaa attaaagttt
     5461 gaacatgatt tttatctctt aaaattgttg ttaaatgtga caaagccttt aaatgactct
     5521 cattatcaat ggctgcaata caaatcaaca atcttacctc ttgttctgga ttatccaata
     5581 aataaatcgg ttcttccaaa actaacattg acattcctat ttcattcaca ccttcatctg
     5641 gccgagcgtg aggaattgct actcccttcc ctaaattaat aaaaggtcca aactcttcta
     5701 ctttttgaat cattgcctca gggtagttct ccgttatctt atcttgatcc aaaagcggtt
     5761 tagctgctaa acgaatcgcc tccttccatc ctaatttttg cgaactaacc tgataggttt
     5821 ctttggtaat aagttgttct agcactggta caatttcctt tctatcattt ttttgataaa
     5881 gataattctt taacgccaat cttaattcca attcttgtgt aataattcca tatcttttga
     5941 caatattcag gatttgttca atctcaaaat ctccatactc taaattcgga aaatctttta
     6001 acactagttc tactagttgt attgcttgct cttcagtcat cataaccgaa actagataat
     6061 ttggcttttc tgtctccacc tttatggtag aaaaaaccat atcatagtca ctactagctt
     6121 tcacctgtaa atcatcaatc tttgaggttc ctataaactc aatttgagga aataatgcta
     6181 atagattctc ttttaacatc aatgaagaac taacaccatt aggacaaatg attgctgctt
     6241 tataccattt ttgaggcaaa gtatctgctt tctttaaata acctccgaaa tggataacaa
     6301 aatatgctgt ttcactatca ggtatgggat tgtcaatagc gtccatcaag ggcatcaaag
     6361 aatctttgac taattcaaat aaatcaggat aatgttcttt aacatgcaat acatattcat
     6421 ttgaactagg taggccgaac tttaatctat agtaagccgg tataaggtgg cgacgaagat
     6481 tttctctcaa tccttccctt tgtttaaaat gtaacaaaga aatatcttcc attctactta
     6541 taatagcctc tgttaattga ttaaagtaaa ccggagcaac atctacttca ccttcaaagc
     6601 aacttgataa taaaactgtg atatagcgat aatcatcctc agaaaacacc gtatctataa
     6661 ttcccaaatc aaccactgta tccaataaaa tagtcgttat atcttgaata acaggagata
     6721 ctaatgtctc tgaaagacat actctttcaa catccctttg atacctacac agaatgaata
     6781 ctaaaccgaa aaggtaaact tttaattgat taacaatagg tactagctgt agcttctcat
     6841 aataatcttt aactacctga tcaatcaaat cataagttaa tgaatatccc caactggata
     6901 aaacataatc caaaccccaa atccctatgg aggattccag caactcacta accatttgaa
     6961 aagctaaacg gtgcttattc cactctgaac cgtgtaaagt ataacctttt gctctactgt
     7021 accctagctc caaatcatta tctaacataa tctttcttaa tgattgaata tcagataagg
     7081 ttgtattctt acttactttc aaaaagtctt ggtaatgatt attcgatata aaatctaatc
     7141 ggcaaaaagt gtaaagatag attaaagcta agcgagtcga ctttggtaaa accaattcat
     7201 ccgacttaat aatatctgtc aaagactgct tcgtacgatt tgataaacta tagcgacctt
     7261 gttttttatc cagcactatc cctttattag ctagataagg cactaaataa tctattcctt
     7321 ctttgacttc ctttataggt aagctcacct taacagataa ttcatataat gatagctcac
     7381 aatgatccat caaagtcatc aaaataacta gtgctctata atcaaacatt tctcttcctt
     7441 tctaactaca attatagtgt aaaaattata agaataaaag agtatttgag cacaatataa
     7501 tctcaccttt taaaagaaat tattgggctg aaagttttta gtaattattt tcaactttct
     7561 attgactttt aaaaacaaag tgatatcatt tagatatcaa ataatgggag gtcattctta
     7621 tgactgaaaa actaatcaat tcaaaaccaa atggtgtatt cgcattgatt ctcattgagt
     7681 tgacaatcgt acttggtatc tttatattta taatgggtgt tggttcggaa aacatttttg
     7741 gaattattat cggaccttta ctaatcgtaa ttgcaggtct agctcatgct ggtttaaaag
     7801 ttgtcaaacc tcaggaagct ctggttctga cactctttgg taattataca ggtaccatca
     7861 aagaacctgg tttttacttt gtcaatccct tcagcgtagc agtcaaccct gcaaaccaca
     7921 ctcgacttgg acaaagtggt gatgttagca caaaatctcc ttttttagga gctaaatcat
     7981 caaatgacaa tgatgtaaat cttgaaattg gcaagaaaca gatttccctc aaagtcatga
     8041 ccttgagcaa ttctcgtcaa aaaatcaatg attgcttagg aaaccctgta gaaatcggta
     8101 tcgcggtaac ttggagagtt gtcgataccg ctaaggcagt cttcaacgtt gataactaca
     8161 aagaatatct ttcattgcaa tgtgatagcg ccctccgtaa tattgtccgc atctatcctt
     8221 acgatgtgtc tcctaatgtg gatactacgg gtgacgggca agcagatgaa ggtagtctcc
     8281 gtggctctag cgaaattgtt gctaaccgta ttcgtgagga aattcaaagt cgcgtagaag
     8341 atgctggctt ggaaatcctt gaagcacgta tcacttacct agcttatgct ccagaaattg
     8401 ctgccgttat gcttcaacgc caacaagcat ctgccattat tgatgcacgg aagatgattg
     8461 tagatggtgc tgtaggaatg gttgaaatgg cactagaacg gctcaatgaa ggggaattag
     8521 tagaacttga cgaagaacga aaagctgcca tggtttcaaa tctccttgtc gttctttgtg
     8581 gcaatcatga tgcacaacca attgtcaaca caggaagtct ttactaaaga tgtctgaagc
     8641 taaaaaaaag cagatccccc ttcgactctc aacaaagtta tacgctgcac tcgcatcatg
     8701 ggcagaagac gactttcgtt cagtcaatgg gcaaatcgaa tatctcctca cagagtgtgt
     8761 caagcaacgg aagaaagacg gaaaatacgt atcagaaacc attgacgaac catttgagat
     8821 tgatatttaa caccttctcc tgtctgccgt gtcggatagg agaatttttt catctttttt
     8881 tgtcaagtaa ctttacttta caaaaaaaat gtgttatcct aatatggttg atgaaaatca
     8941 gtagattgaa tcgaatataa atacctaaag gagaaatcaa aatggcagta cctgcacgtc
     9001 gcacttcaaa agcgaagaaa aacaaacgtc gtacacacta caaagtaaca gctccatctg
     9061 taaactttga cgaaactact ggagattact cacgttctca ccgtgtatca cttaaaggat
     9121 actacaaagg acgtaaaatc gctaaagctg catcagctga ataatagaag ggagatacca
     9181 tgcgcgtaaa tattacactt gaacacaaag aatctggtga acgcttgtac cttacttcta
     9241 aaaacaaacg taacactcca gaccgccttc aattgaagaa atactcacca aaacttcgca
     9301 aacacgttgt gtttacagaa gtgaagtaag cattcaatac gaatcaatac agaagaaaaa
     9361 cgcttgatca gcgttttttc tttttgttaa tttcactata ttgcaaggga aatcattcga
     9421 atgactacat tttttgaaat aagaatacgg attcggacga ttaaaagtaa gagaaactca
     9481 aatgtaagtt ggggttcctt ttttgtatat ttttcaaata acgcctctac actatttgta
     9541 gcaaattcac caactacagt tgcatcttag ttaaaataag ttagaatatg taagtgagta
     9601 ccagatatac caagacatcg tcaccatcta aggtatattc aaaatacaaa agttgaccaa
     9661 ctagatttct gaatatcctt atatatccat tcttaaaatt ggtttaaata gcgtagtctt
     9721 ttaaactagt tttgagaatc caaaaaatct tcctacatat gtaagaagat tttttagttc
     9781 agaatgatta gatttagcta atggatacct atcctaccca ctcaccgtta gcattgactc
     9841 gatagccatc gggtgtagtc gtgtttacta ataaatctcc tgaattatag gtatagtacc
     9901 atttaccaga aactgtaa
//
LOCUS       DAVAAL010000062         9882 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVAAL010000062 DAVAAL010000000
VERSION     DAVAAL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.063"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(56..445)
                     /locus_tag="VQM60_001286"
     CDS             complement(56..445)
                     /locus_tag="VQM60_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET1081152.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(499..1191)
                     /locus_tag="VQM60_001287"
     CDS             complement(499..1191)
                     /locus_tag="VQM60_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET1081153.1"
                     /translation="MNTSFVHAVDGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVIKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTRTRTYDVNPETGHVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDGSTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            complement(1907..2458)
                     /locus_tag="VQM60_001288"
     CDS             complement(1907..2458)
                     /locus_tag="VQM60_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081154.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(2808..3632)
                     /gene="nadE"
                     /locus_tag="VQM60_001289"
     CDS             complement(2808..3632)
                     /gene="nadE"
                     /locus_tag="VQM60_001289"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET1081155.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(3629..5089)
                     /locus_tag="VQM60_001290"
     CDS             complement(3629..5089)
                     /locus_tag="VQM60_001290"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET1081156.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMHNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(5205..5693)
                     /locus_tag="VQM60_001291"
     CDS             complement(5205..5693)
                     /locus_tag="VQM60_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081157.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(5683..5892)
                     /locus_tag="VQM60_001292"
     CDS             complement(5683..5892)
                     /locus_tag="VQM60_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1081158.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(6278..7006)
                     /locus_tag="VQM60_001293"
     CDS             complement(6278..7006)
                     /locus_tag="VQM60_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1081159.1"
                     /translation="MKLNKLNFLKENIRNLYSSGVIYLGLLISFIPPILVTFFILKTQ
                     GTSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTMMMNTSNDLFIKAIELLPFYSLPVFVASNTMSISQ
                     YLVVITTIILLYFFTLKKSKKYSF"
     gene            complement(7022..7813)
                     /locus_tag="VQM60_001294"
     CDS             complement(7022..7813)
                     /locus_tag="VQM60_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081160.1"
                     /translation="MNFALNKGEIVGLAGRNGVGKSTLMKILVQNNQPTSGNIISSDN
                     VGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQEIFRSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKNLALHENVGILISSHKL
                     EDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEE
                     GLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            complement(8249..8977)
                     /locus_tag="VQM60_001295"
                     /pseudo
     CDS             complement(8249..8977)
                     /locus_tag="VQM60_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(9237..9548)
                     /locus_tag="VQM60_001296"
     CDS             complement(9237..9548)
                     /locus_tag="VQM60_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081161.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(9563..>9882)
                     /locus_tag="VQM60_001297"
     CDS             complement(9563..>9882)
                     /locus_tag="VQM60_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HET1081162.1"
                     /translation="IPEYKFVAEVVSYDDAVQTATIRQRNVINEGDQVEFYGPGFRHF
                     ETYIEDLHDAKGNKIDRAPNPMELLTIKVPQPVQSGDMVRALKEGLINFYKEDGTSVT
                     VRA"
BASE COUNT         3007 a         2078 c         1608 g         3189 t
ORIGIN      
        1 atataattat atattcagac atactattat caattttgtc gcagggagga atctgttaac
       61 gcacccattc accattatca ttgactctat agccatctat acttgtattg accgctaact
      121 cacccgatgt atttacataa taccatttac caccaacttg gaaccattga ttgactttca
      181 tagaaccgtt gctgttgagg tagtaccatg aaccattaac ttgtacccaa cctgttgcca
      241 tggaaccatc agtattataa aaataccaca taccattttc ttgtttccag cctgttgttg
      301 gagcaactgc tttagctggt tctactgcta catctgttcc ttggttagat gtaacagata
      361 caggatacga aggaatagat gattgctcag gaacaacaac tttttcaggt tctctcgtcc
      421 ctctccttat acgtcttttt accatctctt tagtaatttg acgagaagta gtttcttcaa
      481 ttgttccatc acgttcatct acagtataga ttgtagtaag agtaatttac caatttctcc
      541 tacttcttct acttcttgac ttttatcaag agttgagcca tcgagatatt ctgtttcgat
      601 tggaatttct tggacaagaa cttggggctt ggttcctttt ttaacaactc ttgtttgaga
      661 gtcttctttt tgacttaaag tactctcagt tacttgtcca ctctttccat ctacatcata
      721 agttatcgtt gtaactgttt tcccattctt tcctagagta atctcttgct cctgtcctgc
      781 agaaaggtca ttgtctgctt catatttagt agcacatgga acaagaactt cttcaacctt
      841 gcttttagct ggaactttga taactgtatc cgtggcttct tttctatcaa cagtaacctg
      901 ttcggtaaca tgaccagtct ctggattaac atcgtaggtc cttgtcctag ttacatagcc
      961 atcctctcca tcaattgtaa caggattttc actacggtct tttgtttcat ctttttcata
     1021 acgaattcgc gtacttgaaa ttttcttgat tactacctta ggtttagtcg ctacttttac
     1081 aataatatcc ccattgtcag cgtcatcata ctctattccc tcttctttat ctctagtatc
     1141 atctctgaca tattgaatcc catcaacagc atgaacaaaa cttgtattca gattcctcct
     1201 aaaaataaag ttagcccgat taccgcagaa ccaaaaatct ttccgagttt acgtaatgca
     1261 tagcgcttat tagtattaga ttttgccatt acatcctact tctagtatag catcttttct
     1321 atcaaacgtt aaacaatata ttttatatat caaatagact tagaatgata tattgattat
     1381 tgaactaaca ctttaactat atcgtaatca atctcatata taaaggattg cagacatctt
     1441 atctaaatac atgctaatat atttagatac aaacattcca acttgataat tttcactcat
     1501 ctttcatcat tccttataca actatgcagt ataaatagaa tagttttctc atcagaatga
     1561 gactatttta atattagata ttttgataga aaaaaacata aataaaattg gttgaattta
     1621 attccctaac tcgatagagg attctatttt aaatttaaca acagctagga ttacactata
     1681 aaaagcacat taccacctgt tcggctggag aatctagtcg attagctccc tcttagttac
     1741 aagcttcaag cgatttttta actaatacta aaccagatta aagaagaaac tagaacatct
     1801 gtcttgtact aatcaatatg ctattttaaa gttgcaaata aacttggaat gctaattatt
     1861 atatttacaa taaatagttg acttcgatag aaacagaaaa tagaattcaa cattcttccc
     1921 tacttatacc ataataaata agatccgcaa gatagccttt tctctctaca ccattaacaa
     1981 tggtttgtag ataggacatt ccagcctttt ccatgacacg acctgaagct gggttgagac
     2041 tggcataacg tgctctgacc ttttgaaaac ctgcttgagt aaaacaaaag tccaagacag
     2101 ctttcaaggc ctctgtcatc ataccatgtc cccagtaagc cttgcctagc acatagccaa
     2161 tttcacagct taaatcagcc tcgtctatct taacaatgct gatatctcct atcacttgct
     2221 ctgggttttc ttttagacaa atggcccatt tgtaatagtt gagattagta taggaagcaa
     2281 cccaattgcg aatcgagttt cgagtgattt cgacatcagg atggggatcc caggtaacat
     2341 aggtcaaatt ctcagcggat gaagcccaat tttgaaacat ggcttctgca tcactctcca
     2401 caaatcttct taaaatcaaa cgttctgtct gtaatatttg cgtaccgata gatttcatga
     2461 tgctacctca cttccttatt tctatgctcg tagtaaaaag actcaactca gtaacagcta
     2521 gaaaacgagt ttgtcttcta atttcttgac gcagtcgctg cctggtttga aatgcgctct
     2581 agcaagcttt ttcaaaccta gtcatcgttg caggggtaag acgatgaaat cgaactcttc
     2641 gagttactga atctgcctca ttttcttatt tctaaactcg gggttcaaga tcccccagac
     2701 cttctctcac tttcttattt caagatgaga ggctgaaaac ctccagtgga ggtttttctt
     2761 gctttttaat ttcagggtaa gaagctaaaa aggtcccccg gaccttttta ctcccaaaag
     2821 tcatcaaata cggtgatggg taagtggcgt ttgtgttggc ctttgtgcca ccagttttca
     2881 atggtcgctt gagcttctgg gctgattgtt ttgccttcta ggtagtcgtc aatctctgcg
     2941 taggtgactc caagtgcgac ttcgtcagct aggcctggtt tatcttcttc taggtctgcc
     3001 gttgggattt tttcataaag ggctggctct gcgccaagtt tctgcaagag ctgttttcct
     3061 tggcgtttat tgaggcggta aagagggaga atatccgcac cgccgtcacc aaacttggta
     3121 aagaaacctg tgatattttc cgcggcgtgg tctgttccaa tgaccgctcc gctatgggaa
     3181 ccagcaaggg catactgagc aatcatacgg caacgtgcct tgatattccc cttgttgaag
     3241 tctgaaacag gactacctgt cgcttcaact gcagctgtca tggcatcagc tgattccttg
     3301 atattcacaa ccaagctgac atctggctgg atgaaggcta gggctttttg agcatctgct
     3361 tcatcagctt gcactccgta tggcaggcgg acagcgataa atttgtagct atcgtctccc
     3421 gtttcagctc gcagttcttc catagctaat tgcgccaaac gtcctgccaa ggttgagtct
     3481 tgtcccccag aaatccctag tacaaaggtt tttaggaagg gatgtttttt cagatatctt
     3541 tttaagaaat caatagaacg acggatttct tcctgggcat caatcactgg tttgacaccc
     3601 agctcttgga taatcgtttc ttgcaaactc attcttcttc tccttcacca agggcttcct
     3661 tgcgcatctt atcaatcaag tccatcttat cttgccatac atcacgcgcc aaatccactg
     3721 gatagtgctg cggattgagc acacgcttat actcatccca caacttgtca aattccttac
     3781 gggcataatc ctgaatgtca gtcaaactag gcaagttgta aactaatatt ccttctttga
     3841 agatatccac caagagagga acggcatcaa aattacgaac cgtcttcttg atgtatgtat
     3901 aggtcggatg gaacatcttg atttctgtca tgtcgctaat atccacacca tcataagtga
     3961 tgtagtcacc ttctgacttg cctttttcac gactggtaat gcgccacacc tgcttcttac
     4021 ctggcgtcga cactttttcc gcattattag acagcttaat cgtattgtgc atctgaccag
     4081 tttcatcttc aattgcaaca atcttgtaaa ccgccccaag agccggctgg tcataggctg
     4141 taatcagctt ggtacccaca ccccagacat caatcttggc cttttgcatc ttgaggttaa
     4201 ggatggtatt ttcatctaga tcattagaag cataaatctt agcctctgta aatccagcct
     4261 cgtccagttg ctgacggact ttcttagaaa tgtaggcaat atccccagag tcaatccgca
     4321 cacccataaa gttaatctga tcacccagct cacgcgccac ctgaatggca gctggtacac
     4381 cgatgcgaag ggtatcatag gtatccacaa gaaagacaca atttttgtgg gtcgcagcgt
     4441 aagccttgaa agcctcatag tcattgccat aaacctgtac caaggcatgg gcatgggttc
     4501 ccaaaacagg aatgtcaaag agcttacccg cacgcacgtt gctggttcca ttggcgccac
     4561 caatcaccgc tgcgcgtgtt ccccagatgg ccgcatccat ttcttgagcc cgacgtgtcc
     4621 caaactccat caagggttca tcttcgataa ccgaacgaat acgagctgcc ttcgtcgcca
     4681 ccaaggtctg gtagttgacg atgttcaaaa gagccgtttc gaccaactga cattgggcta
     4741 gaggtccttc cacctgcaca atcggttcat tagcaaaaac caaatcccct tcttgggcag
     4801 aacgaacggt caactccaac ttgaaattgc gaaggtaatc caagaacgcc ccatgataac
     4861 caagcgactc caaataggct atatcactat ctgaaaaacg caagtcttca agatagttca
     4921 caattctttc caaacctgca aaaaccgcat agccgttctt aaaaggctgt tggcggaaat
     4981 acacctcaaa gaccgccttc ttattgtgaa tcccttggtc aaagtaaacc tgcatcatgt
     5041 tgatctggta caagtccgtg tgcaatgtca aactatcatc tggatacata cttttcctac
     5101 ttccttagct agaaacccat gaaaattttc aagaactttc atgtattcca ataaattagt
     5161 actattatat cacattttag ctggattgag aaaagagtaa caagctattc tccactctcc
     5221 aattcatcca tatcttgttc aaattttttc tgagcccatt cgccatagct cttaagacca
     5281 agattgccaa taaagaccca cggaaggtaa atgacataag taatgaccca agcagacagg
     5341 tatttaaaat tcaaaggatt gtgctgataa atttctatgt tgaattgata attctgcaac
     5401 atcaaaagag ccgtaatagc caaggttagg aaaaaacaac ccaaaatcgt aaaatgaaaa
     5461 cgactatagt aggtcactcc cagataacgg gcacggttaa aaaagtaaaa tgtccctatg
     5521 atgataacga ttagcagcat attagaatta aaaaggcttg gtgctaatac tgaaatgata
     5581 taagatagga gcgacaaagc gatgcagata tagaaacttt cagagcccgc tttattgatc
     5641 agttgttctt ctctttcgtc tagtaattga taataataaa atctattttt catcttcttc
     5701 ctcccaaaat agttggtcta gggttttccc taaacatctg caaatagact ggcagagcga
     5761 gagactggga ttgtattttc ccgcctctat caaaccaata gtctggcgtg tcaccccgac
     5821 agcctctgcc agttgacctt gtgttaaatc acgctctacc cgagctaatt ttaattttaa
     5881 atttttagcc accttcgtcc tccttatagt tttaatactc atctactctt aaaaaatcca
     5941 aaaccaacac aagctatcag ctatttatta taaccccatt aatttgcact ccagtattat
     6001 cttcagccga aaatcagtat tttaggttgt ttgaaataaa cttcctagtt tactctttta
     6061 tttttattta gtattagctt tagtaggatc ttttaaacga gtttttattg cttatatttc
     6121 ttgtttagtt tagttggttt catatcacta ttatataatg cttttggaaa tgagcctagt
     6181 ataagtcaaa aacgtgttga atagtgaaag caggagcata ccaagatgac tgattttttt
     6241 attgtatttt aggtatgcaa aataatacta aatgaaacta aaatgaatac ttcttgcttt
     6301 tcttgagagt gaaaaagtac aataaaatga ttgtagtgat taccacaaga tactgactaa
     6361 tagacatcgt atttgaagcc acaaatacag gtaaagaata aaaaggaaga agttcaattg
     6421 ctttgataaa caaatcatta ctagtattca tcatcatcgt atttaatata ttagggataa
     6481 agagtagtaa agctactaaa ataccaaata ccaagccact tttcaatttg tataaataga
     6541 aaatgtaaga taatagaaaa taaaacagga ctaacaaaat tgtattgact acaatagcta
     6601 ctataaaatc taatcctaaa tcacctggaa tacccaattt acttactatg actagtaaca
     6661 caatgccatt caaagcataa ataatggaaa ctgagatggc atatagaaaa ttagacaaga
     6721 cgtaaatcat ccgattcttc tgttgattat aaaacaaact taccgtattg tgggaaaaat
     6781 ctctactaat gactcgatta gcatgtataa cagccattaa cataccgagc atagcataaa
     6841 agtttgaaat atgcttaata ccaagcgatg tcccttgagt ctttagaata aagaatgtaa
     6901 ccaatatcgg cggtataaac gagataagca aaccgagata tattacgcct gatgaatata
     6961 aatttcttat attttcctta agaaaattca atttatttaa tttcatgatt ataaccttcc
     7021 tttattttga acgatttagg taaatatctt taagcgtctc ttttttagtt tcaaaatcaa
     7081 ctactttaat agagttttcg ttaaaaaatt taaaaagctc actactttga atattcccag
     7141 acatagtaat tctcaatccc tcttcctgaa caatatctcc aaattcttgt ttggtaatga
     7201 aaatgtctct atctgtagct gatgaaaaag ctatctcaaa caagaaatta tgactatctt
     7261 ttcctacttt ttgaaatgtc aaaagcccgt tctccaagaa aagaactctc tcacaaattt
     7321 cttcaatgtc ttctaattta tgactcgata ttaaaattcc cacattttca tgtaaagcta
     7381 aattttttag aaccgctaaa actatttgtg atgattcaat atctaaacca ttagtcggtt
     7441 catctaaaat caatatatca ggttccgtaa cgagagttag aagcaaagct aatttttgtt
     7501 ttgtacccaa agaataggtc tttacttttt tattaataga ctgagtcaaa tctaactctt
     7561 ggatcaaact tctaaatatt tcttgattgt agtcaacacc atataaattt gacaaatatt
     7621 ttaaattctc taaacctgtt ttagataaaa ataattttgg ttcttcgatt aaatacccaa
     7681 cattatcact gcttataata ttacctgaag tcggttgatt attctgaaca agaattttca
     7741 tcaacgtact cttaccaact ccatttctcc ctgctagacc aacaatttca cccttgttaa
     7801 gtgcaaaatt tatatctttg agaatgattg aatgaccata atgcttactt acatttataa
     7861 cttacatttt ttattcctcc attaatattc atggtttgct ctttgattct tattgagttt
     7921 aaaaagttgc acctcttttt aacaatatta tatcatatat ctaactcaat gcaatatata
     7981 aatgtcattt tgtaaaattt tattaacaaa aaactctcta caacaaaata atagagagtt
     8041 ttctgcttat taattttttt caaaatatct aagacatcgc ttctttgttt aatttgatag
     8101 gctttaatac ctaagctccg actttatcac ttaaaaaata aaaaaggaca gctaatctct
     8161 tgcgcatcaa gggatttcgc cattctttaa tctcgtttaa gtgttgtata tggagctttg
     8221 cataattttt taaaactaaa caactacatt aagcacgaac tgtgacgctg gttccatctt
     8281 ccttataaag attgataagc ccctctttaa gagctcgaac catgtctcca gcttgaacag
     8341 gttgtgggac tttaatagtc aatagttcca ttggatttgg agcgcggtcg attttattgc
     8401 ctttggcatc atgcaaatct tcaatatagg tttcaaaatg acggaaacct ggaccataaa
     8461 actcaacttg gtccccttcg ttaatgacgt ttcgttgacg aatagttgct gtttgtgccg
     8521 catcatcata agaaaccact tcagcgacaa acttgtactc agggatttta cgacgagcac
     8581 caaacaactg ctcattttca gatggtgtac catagtaaaa tcctgtagcc agttcacgtt
     8641 gggcaacctt ccacatctcg tccaccaagt cttgtttgat agcttcaaac ttttcaggac
     8701 tttcaagata ggcatccaca gccgccttgt aacagttggt tactgttgat acatagtgaa
     8761 tagactccat acgtccttcg atttttagac tgtccacacc attttcaatc atatctgaga
     8821 tatggtcaat catagacata tcaacggctg acattgaaaa ttcttctgga atctcaccct
     8881 gcaaactctt acgttctttc ccaaatggca tatcgtaaag gtcgtacttc caacggcatg
     8941 actgagaaca tccaccacgg ttggcatcac gcatactcta gacggattgt tttgacaatt
     9001 ctaacctttt agactttgca taataactgt taaacagtcg aaaattaccc cagttttaac
     9061 aagtagtcaa aataccagat aaagtaaaaa ggggctagga aaaaagtctt taaaatctta
     9121 aaaacgcata gtatcaggta ttgaacaatc ttgatattat gcgttttatt gtgggaagat
     9181 ttacttcatt ttctcttgga attgagtttt ctcctacttc aaaactagat gatttgctat
     9241 ttaaaatagc gttccttatg attttggcaa cctggattaa agcgagaaaa acaaaagagg
     9301 caacttaagc tttctttata ttgcttatat gttaatagtt ttagacaaac accacataaa
     9361 ataggcttat cctgtatcaa agataaggga taaggctcaa aagggtgatt ttctaatgaa
     9421 tcatgacacc gataacaagc ataatacttt ttacaatcat agcattgtaa agcaataata
     9481 tccagcttgc tatggtaatg aatgcatctt gtttgcttat ccactaagaa accctgtgcc
     9541 tctatcattt ccttttcctt tcttaagctc gaactgtgac gctggttcca tcttccttat
     9601 aaaaattgat gagtccttct tttaatgcac gaaccatgtc tcctgattga acgggttgtg
     9661 ggactttaat agtcaatagt tccattggat ttggagcgcg gtcgatttta ttgcctttgg
     9721 catcatgcaa atcttcaata taggtttcaa aatgacggaa acctggacca taaaactcaa
     9781 cttggtcccc ttcgttaatg acgtttcgtt gacgaatggt tgctgtttgt accgcatcat
     9841 cataagaaac cacttcagcg acaaacttgt actcaggaat tt
//
LOCUS       DAVAAL010000063         9585 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVAAL010000063 DAVAAL010000000
VERSION     DAVAAL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.064"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(34..231)
                     /locus_tag="VQM60_001298"
     CDS             complement(34..231)
                     /locus_tag="VQM60_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET1081163.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(306..1082)
                     /locus_tag="VQM60_001299"
     CDS             complement(306..1082)
                     /locus_tag="VQM60_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET1081164.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            1204..1950
                     /locus_tag="VQM60_001300"
     CDS             1204..1950
                     /locus_tag="VQM60_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET1081165.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1963..2943
                     /locus_tag="VQM60_001301"
     CDS             1963..2943
                     /locus_tag="VQM60_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET1081166.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3138..3458
                     /locus_tag="VQM60_001302"
     CDS             3138..3458
                     /locus_tag="VQM60_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET1081167.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            3504..3812
                     /locus_tag="VQM60_001303"
     CDS             3504..3812
                     /locus_tag="VQM60_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET1081168.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3829..5127
                     /locus_tag="VQM60_001304"
     CDS             3829..5127
                     /locus_tag="VQM60_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET1081169.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLAMSTMVYFPFFKIQDRLACQNEIKQS"
     gene            5278..7716
                     /locus_tag="VQM60_001305"
     CDS             5278..7716
                     /locus_tag="VQM60_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET1081170.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7735..8403
                     /locus_tag="VQM60_001306"
     CDS             7735..8403
                     /locus_tag="VQM60_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET1081171.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            8421..9509
                     /locus_tag="VQM60_001307"
     CDS             8421..9509
                     /locus_tag="VQM60_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET1081172.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
BASE COUNT         3165 a         1337 c         1933 g         3150 t
ORIGIN      
        1 tagagtaaaa ttctctacaa aaagaagaat agcctatttt actattcttc cgagtgattt
       61 caatttcttt ggggaaatat ggagatactt tttaaatact ttggcaaaat atttatagtc
      121 tgaaaatcca acttggtcag agatagcgct taaaggggag ttgggagatt cagccatctt
      181 atctatagct tgtttcaggc gatattgtaa gatatattca tttagagtca tggggaagtc
      241 ttctttgata accttatata gatgcttgtt atctgcaaat agctagagca tttcaaggaa
      301 ataaactaga aataacaatt aatatggtgg ttcagaaata ccttttgata atcaataaga
      361 tcttcaggat gaagtgcgtt gactccatcc atttcatatt tccgatttaa taaacgatat
      421 ttgttttcac caaattgatg aaaagggagt agttgaactt ggtcgatatt taatgagtta
      481 aatagagtag cgaatttttc tgcatcctct aaactattgt taaaattagg aataactggg
      541 attcttaaaa cgatagtttt attttgagaa aaagcataat gaatgttttt aataatcatt
      601 tgattaaaaa ccccagtcac ttttttatgt tttatagaat tataatgttt taggtctgtg
      661 tagataaaat ccacatattg aattaaatca ataaattttt catgatcaac aaaggcagta
      721 gtttcaatgg cagtgtgtat gtgatgttct ttagctgatt ttaagatggc tttagcaaat
      781 tcaaactgag caaatatttc acctcctgat aaagttaaac ctccaccgga ttcttcgtaa
      841 aattctttgt cttttaatac ctctgtaata atttcttcta cagtcttttc ttcaccgact
      901 aaggtgaatt tctctcgttg agcatctttc attttttcag gtttcattct ttgagattca
      961 ggattagaac accatggaca gcgcagagga catcctttta aaaaaacagt tgtacgaata
     1021 cccgggccgt catgaattga aaagtgttga atattaaaaa taattccttt agatatttcc
     1081 ataagagttc ctcctcttca taagtctatc ctatcacaaa aacgaaagaa aaacaattac
     1141 gaacgaaagt ttaatttgtt gttttttgct ttttattttg gtagaataaa acggaggtgt
     1201 aaaatgaaaa gattggaaca aattattaaa ttagtatcag aatatgaaaa gatcgacgtt
     1261 aatacattat cggaaaaatt aaatgtatcg aaagtaacga ttagaaaaga tttagataaa
     1321 ttagagtcaa aaggtttatt acacagagag catggatatg ctgtattaaa tagtggagat
     1381 gacttaaatg tacgtttgtc aattaattat gaaattaaga gaaaaattgt tcaggaagca
     1441 gtaaaattgg tgtcagataa tgaaacaata atgatagaat ctggatcgac ctgtgcttta
     1501 cttgctgagg aaatttgcaa gcaaaaaaga aatgttacga ttgtaacaaa ttcatttttt
     1561 atagcaaatt ttgtgagagc ttatgattca tgtcgtatta ttgttcttgg tggagagttt
     1621 cagaaggatt cacaggttac tgtgggacct ttattaaaag aaatgataca gacttttcat
     1681 gtgcatcaag cttttgttgg gacagatggt tacgataaag agatgggctt tacaggaaaa
     1741 gatttaatgc gcagtgaggt agttcaatat atttcagcag cgtcggataa agtcattgta
     1801 ctaactgact caagtaaatt tgataaaagg ggtacagtaa gaagatttgc tttaagtcaa
     1861 gtctatgaag taataacaga cgaaaaactt tctaaacaaa atatagctac attagaaaat
     1921 gctgggataa tggttaaggt agtttcgtaa gaggttaagt gtatgaatca agataggaat
     1981 aaactgctct ctaaaattgc ttatctgtat tatattgaaa acttaaatca gtcacaaata
     2041 gcagcaaaat taggaattta tagaacctct attagtagaa tgttaacaga agcaaggaat
     2101 gcaggaattg ttaaaattga aatagagaat tttgatacca atatgtttaa gttggaaaat
     2161 tatgtaaaag aaaaatatgg tctggaaagt ttagaaatta ttccaaatga atttgatgat
     2221 aatccaacaa ttttatctga aagaatttct caagttgcag caggtgtcct taggaatcta
     2281 attgatgata atatgaaaat tggcttttct tgggggaaaa gtttaagtaa tttagtagat
     2341 ttaattcaca gtaaaagtgt ccgaaatgtt cacttctatc ctctagcagg tggtcctagt
     2401 cacatacacg ctaaatacca tgtgaataca ctgatttatg aaatgtctag aaaatttcat
     2461 ggagagtgta catttatgaa tgcaacgatt gtgcaagaaa ataaattgtt agcagatggt
     2521 attttgcaat caagatattt tgaaaatttg aaaaatagtt ggaaagattt agatatagct
     2581 gtagtcggaa ttggtgattt tagcaataaa ggaaagcatc aatggttaga catgcttaca
     2641 gaggatgact ttaaagaatt gaccaaagtt aaaactgtag gagaaatttg ttgtcgattt
     2701 tttgattcaa aaggtaaaga agtctatgaa aacttacagg aaagaacgat agcaatctct
     2761 ttggaagatt taaaaaatat tcctcaaagt ttagctgttg cttacggtga tacgaaagta
     2821 tcttcgattc tttctgtctt gcgtgctaat ttagtaaatc atttgattac agacaaaaat
     2881 acaattttaa aagttttgga agaagatggg gatttgactt ttagagagat tctaggtgag
     2941 tgaaaatgat agactgattc agtttatcgt ttttcttttt agttgattgc acatttgtgc
     3001 ttatataaac aaaaatagtt tatctgttgt ttttggattg acaactttat tatgtagttg
     3061 tattctatag ttacaaaaga aaattttaaa atttcaaatg aaaaaagctt tttacatagt
     3121 gaaatgagga ggaatttatg gaaattattg ttccagatca aattatcatg ggtttaattt
     3181 tatatgctgg cgatgcgaaa caacatattt ataaagcatt agattacata aagaacggta
     3241 catgtgaacg atgtgaagaa gaaatccagt tagctgatgc agccttatta gaagctcata
     3301 atctacaaac aaaattcttg gcacaggaag cgtctggtac aaagacagaa attacagctc
     3361 tctttgttca ttcacaagat catctcatga ctagtatgac ggagattaat ttaatcaaag
     3421 aaattattag tttgagaaaa gaacttcata aaaaataata ctagagtatt atcattgtta
     3481 ttaacataga ggaggaaaac ataatggtga agattggttt gttttgtgca gcaggttttt
     3541 ctactggtat gcttgtaaat aatatgaaaa ttgcagcgca atctagtgga gttgaggcag
     3601 aaatagaggc gttttctcag tctaaattag cggattatgc gccaaatata gatgttgcac
     3661 tattgggtcc acaagttgct tatacattag ataaatcaaa agaaatttgt gataagtgtg
     3721 atgttccgat agctgttatt ccgatgatgg actatggtat gttagatggg aaaaaagtat
     3781 tagatttggc cctatctttg attagtgggt aagaaaagga gatttattat gtcaaagatg
     3841 gatgttcaga aaatcattgc accgatgatg aagtttgtga atatgcgtgg cattatagct
     3901 ctaaaagatg ggatgttagc aattttgcca ttgacagtag ttggcagttt gttcttgatt
     3961 atgggacaat tgccgttcga aggattaaat aagagcattg ctagtgtttt tggagctaat
     4021 tggacagagc cgtttatgca agtatattca ggaacttttg ctattatggg tctaatttct
     4081 tgtttttcaa ttgcctattc ttatgctaag aatagcggag tagaggcttt accagctgga
     4141 gttctatctg tatctgcatt ctttattttg ctaagatcat cttatatccc taaacaaggt
     4201 gaggcgattg gggacgctat tagtaaagtt tggtttggag gccaaggaat tatcggtgct
     4261 atcattatag gtttggtagt aggaagtatt tataccttct ttataaagag aaaaattgtt
     4321 attaagatgc cagaacaagt tccacaagct attgccaaac agtttgaagc aatgattcca
     4381 gcatttgtaa ttttcttatc ttctatgatt gtatatattt tagcgaagtc attgactaat
     4441 ggcggaacat tcatagaaat gatttattct gctattcaag ttccgttgca aggtttaact
     4501 ggatctttgt atggtgctat tggaattgca ttctttatat catttttgtg gtggtttggt
     4561 gttcatgggc aatcggtagt aaatggagta gtgacagctc tgcttttatc taatcttgat
     4621 gctaataaag ctatgttagc ctctggtaat ctatcattag aaaatggtgc acatattgtt
     4681 actcaacaat ttttagattc atttttaatt ctatcaggtt cagggattac gtttggtctt
     4741 gtagttgcca tgctttttgc agcaaaatca aaacaatacc aagccttagg aaaagttgca
     4801 gcttttccag caatatttaa cgtaaatgag ccagttgtat ttggatttcc gattgtcatg
     4861 aatccagtta tgtttgtacc tttcattctt gttcctgtac ttgcagctgt gatagtatat
     4921 ggagctattg caacaggttt catgcaacca ttctcagggg taacattgcc ttggagtaca
     4981 ccagctattc tatcaggatt tttggtgggt ggatggcaag gtgttgtgac tcagttatta
     5041 gtattggcga tgtctacaat ggtttacttt ccattcttta agatacaaga tcgtttagct
     5101 tgccaaaatg aaatcaaaca atcttagagg tatttgtgtg ttactgttaa actcacacat
     5161 ttgtgctaaa aattagagag ttaaaatttt tctagttaaa agcttgaaaa tttctataaa
     5221 aatcggtatt atattttcga aagaaataaa aacattttcg aaagaaaggt gcttacgatg
     5281 gtaaatacag aagtagcaag aacaacaatc aagacagaat attttggcag ccttactgaa
     5341 aggatgaaca aatatcgaga agatgtttta aataaaaaac cttatattga tgctgagaga
     5401 gcagttctag caacacgcgc ctatgaacga tacaaggaac aacctaatgt cctaaaacgt
     5461 gcatatatgc tgaaagaaat tttggaaaat atgtctatct atattgaaga agaatctatg
     5521 attgcgggaa atcaagcttc ttccaataaa gatgctccta tttttccgga atatacgcta
     5581 gaatttgttc tcaatgagtt ggatcttttt gaaaagcgtg atggagatgt tttctatatt
     5641 acagaagaaa caaaagaaca acttagaagt attgctccgt tttgggaaaa taataattta
     5701 cgtgctagag ctggtgcctt attacctgaa gaagtgtctg tttatatgga aacaggattc
     5761 ttcggtatgg aaggtaagat gaattctgga gatgctcact tagcagttaa ctatcagaaa
     5821 cttttgcaat ttggtttaag aggttttgaa gagcgggctc gtaaagcgaa ggtagctcta
     5881 gatttaacag atccagcaag tattgataaa tatcattttt acgactctat atttatcgta
     5941 atcgatgcta ttaaagtata tgcaaagcgc tttgttgctc ttgctaaaag tttagccgaa
     6001 aatgcaaatc ctaaacgtaa gaaagaatta cttgagattg cagatatttg ctctagagtc
     6061 ccatatgaac cggcaactac ttttgcagaa gctattcaat cagtttggtt tattcaatgt
     6121 attttacaaa ttgaatctaa tggccactct ctttcatatg gccgttttga tcaatatatg
     6181 tatccatata tgaaggctga tttagaaagt ggtaaagaaa cagaagatag cattgttgaa
     6241 cgtctgacaa atctttggat taagacaatt acaattaata aggttcgcag tcaatcacat
     6301 acattttctt cagcaggaag tcctttatat caaaatgtta caattggtgg acagactcga
     6361 gataagaagg atgctgttaa cccattatct tatttggtat taaaatcagt tgcacaaacc
     6421 catctaccgc aacctaatct aactgtacgt taccatgcag gtttagatgc tcgtttcatg
     6481 aatgagtgta ttgaagtgat gaaacttggt tttggtatgc ctgcatttaa taatgatgag
     6541 attattattc cttcttttat tgcaaaagga gtattggaag atgatgctta tgattacagt
     6601 gccattggat gtgttgaaac ggcagttccg gggaaatggg gctatcgttg cacaggtatg
     6661 agttatatga acttccctaa ggttctactt atcacgatga atgatggaat tgatccggct
     6721 tcgggtaaac ggtttgcacc aagctttggt cattttaagg atatgaagaa cttttctgaa
     6781 ttagaaaatg cttgggataa aacactaaga tatttgacac gaatgagtgt tattgttgaa
     6841 aattctattg atttatcatt ggaacgagaa gttcctgata ttctatgttc agcattgact
     6901 gatgattgta ttggtcgtgg aaaacacctt aaagaaggtg gagcagtata tgattatata
     6961 tcaggattgc aagttggaat tgcaaatttg tcggattcat tagctgcaat taaaaaattg
     7021 gtgtttgagg aagaacgtat aagcccaagt cagctttggc atgcactgga aacagattat
     7081 gccggagaag aaggtaaggt cattcaagaa atgttgattc atgatgcacc taagtatggt
     7141 aatgatgatg attatgctga caaattggtt actgctgctt atgacattta tgttgatgaa
     7201 attgctaaat atcctaatac acgttatgga agaggtccaa ttggaggaat tcgttattca
     7261 ggaacatctt ctatctcagc caacgtaggg cagggacgtg gaacattagc aactccagat
     7321 ggacgcaacg cgggtacacc gttagcagag ggttgttcac catcacataa tatggatcaa
     7381 cacggcccta catctgtttt aaaatctgtt tcaaaattac caacagatga aatcgtaggt
     7441 ggggttctct taaatcagaa agtaaatcct caaacgttag ccaaagaaga agataaatta
     7501 aaactaattg ctttgttacg aacattcttt aatcgtttac atgggtacca tattcaatac
     7561 aatgttgttt ccagagagac gctgattgac gctcagaaac atcctgaaaa acacagagac
     7621 ttaattgttc gtgttgcagg atactctgca ttcttcaatg ttctttctaa ggcaacccaa
     7681 gatgacatta taggacgtac tgagcatact ttgtaaaata aagaggttct ttttatggaa
     7741 tttatgcttg acacattaaa tttagatgag attaaaaagt ggtctgaaat tttgccgcta
     7801 gctggggtaa cttcaaatcc cactattgca aaaagagagg gttctattaa tttttttgaa
     7861 cgaatcaaag atgtaagaga attgattggc tctacaccct ctattcatgt tcaggtgatt
     7921 tctcaagatt ttgaaggcat cttaaaggat gctcatgaaa ttcgaagaca agcaggagat
     7981 gatatattta tcaaagtacc tgttactcca gctggattac gtgcaataaa ggtgctaaaa
     8041 aaagagggct accatatcac tgcaacagct atttatacag ttattcaggg attattagct
     8101 atcgaagcag gagcggatta cctagctcca tattataata gaatggaaaa tctgaacatt
     8161 gattcaaatt ctgtcattcg tcaattagct cttgctattg atagacagaa ctctcctagt
     8221 aagattttag ctgcatcctt taaaaatgta gcacaagtaa ataatgcttt agctgcaggt
     8281 gcgcatgctg ttacagcagg agcagatgtt tttgaatcag ctttcgccat gccatctatc
     8341 caaaaggcgg ttgatgattt ttctgacgat tggtttgtta ctcaaaatag tcgttccatt
     8401 tagatagaga ggaaatacat atgagaattt ttgctagtcc ttctagatat attcaggggg
     8461 aaaatgcctt gtttgaaaat gccaaatcaa ttttggattt gggaaattac ccgattctat
     8521 tatgcgatca gttggtttat gatattgttg gaaaacgatt tgaagattat ctacataggt
     8581 atggtttcca tattgttctg gcgctattta atggtgaagc ttctgacaat gaaatcaatc
     8641 gagttgttgc cttggctgag aaagaaaatt gtgatagtat tatcggtctt ggtgggggaa
     8701 agacgattga tagcgcaaaa gctattgcag atttgattga aaagcctgtt attattgctc
     8761 caacaattgc atcgaccgac gcacctgtat ctgctttatc tgttatttat acagatgaag
     8821 gtgcatttga tcattatcta ttttattcta aaaatccaga tttagttttg gttgatacaa
     8881 aagttatttc acaagcccct aagcgtttat tagcgtctgg tattgcagat ggtttagcaa
     8941 cttgggttga ggcgcgtgcg gttatgcagg caaatggaaa aactatgttg ggacaacagc
     9001 aaacattggc tggagttgca attgcgaaga aatgtgaaga aacgctgttt gcagatggtt
     9061 tacaggctat ggcagcttgt gaagctaaag tggtgacacc agcattagaa aatattgttg
     9121 aagctaatac tttattgagt ggtctaggtt ttgaaagtgg aggattagct gcggcgcatg
     9181 caattcataa tggttttact gcattgacag gtgacattca tcatttaaca catggtgaaa
     9241 aagtagctta tggaacttta gtacaactat tattggaaaa tagacctaaa gaagaacttg
     9301 ataagtatat tgagttttac aaaaaaattg gtatgccaac aactctaaaa gaaatgcatt
     9361 tggatcaagt tggatatgat gatttaataa aagttggtaa acaagcaact atggagggtg
     9421 agacaattca tcagatgccg tttaagattt cgccttcaga tgttgctcaa gctattatcg
     9481 ctgtagatgc ctatgtaaat tcaaaataaa caataaggac tactgttttc caaatggtag
     9541 tcttttattg atccctgtat tgaattctat agaagattga aatag
//
LOCUS       DAVAAL010000064         9552 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVAAL010000064 DAVAAL010000000
VERSION     DAVAAL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.065"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            170..265
                     /locus_tag="VQM60_001308"
                     /pseudo
     CDS             170..265
                     /locus_tag="VQM60_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(518..1138)
                     /locus_tag="VQM60_001309"
     CDS             complement(518..1138)
                     /locus_tag="VQM60_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET1081173.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1138..1620)
                     /locus_tag="VQM60_001310"
     CDS             complement(1138..1620)
                     /locus_tag="VQM60_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET1081174.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1620..2462)
                     /locus_tag="VQM60_001311"
     CDS             complement(1620..2462)
                     /locus_tag="VQM60_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET1081175.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2557..3345)
                     /locus_tag="VQM60_001312"
     CDS             complement(2557..3345)
                     /locus_tag="VQM60_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET1081176.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQALK
                     HLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDKTYKS
                     HITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTMSNMN
                     EGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLESSPKT
                     DLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3489..4868
                     /gene="glmU"
                     /locus_tag="VQM60_001313"
     CDS             3489..4868
                     /gene="glmU"
                     /locus_tag="VQM60_001313"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET1081177.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLTGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4878..5423
                     /locus_tag="VQM60_001314"
     CDS             4878..5423
                     /locus_tag="VQM60_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET1081178.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5435..5749
                     /locus_tag="VQM60_001315"
     CDS             5435..5749
                     /locus_tag="VQM60_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET1081179.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5766..6458
                     /locus_tag="VQM60_001316"
     CDS             5766..6458
                     /locus_tag="VQM60_001316"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET1081180.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYSYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHFGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6535..7026
                     /gene="rocS"
                     /locus_tag="VQM60_001317"
     CDS             6535..7026
                     /gene="rocS"
                     /locus_tag="VQM60_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET1081181.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7124..7714
                     /locus_tag="VQM60_001318"
     CDS             7124..7714
                     /locus_tag="VQM60_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET1081182.1"
                     /translation="MERLEDYIAFDLEFNQHEGQKHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     YGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7723..9486
                     /locus_tag="VQM60_001319"
     CDS             7723..9486
                     /locus_tag="VQM60_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET1081183.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         2966 a         1654 c         2041 g         2891 t
ORIGIN      
        1 atttttttaa cttgatactc agtgaaaagc aaagattaaa ctaggaagct agctgtaggc
       61 tgctcaaaga acagctttga ggttgcagat aaaacttgtg aggtaaccaa catatataat
      121 gtgaagctga cgtggtttga atagatttta gaagggtatg agtctggaag ttttaatgga
      181 taatgcaaga ttccatagaa tgggtaagct agagttctta tgtgaagagt ttgggcataa
      241 acttttacct tttcctccct actcatctta gtatagaaaa gtgaatctaa aatagtacat
      301 aactgcttct aaaacattct tataaattga tttaaattct caaatcatat tattcagttc
      361 ttatttcatt ttgctctaca atcctgttga gaagacacgt gttcatatca aaaaggtatt
      421 ggcaagttgc aatacctttt tacaaggttc ttttgtctta tttttgtttc aactgactat
      481 atctcctatg gttctagttc agaaggctag gctataatta tgattgataa gaagtatcat
      541 tccaagtatt gagagtgaat gtttcaaaat catgggtttc tataatggtc aggctggcat
      601 ttgctagacc gccatcttta cgaagaagtg gttctttata gcctaggaga gtacgaagac
      661 tggcagtaag attggcgccg tgtccgacaa ttagaatacg ctcagctgga ctatctttta
      721 atgatttgat aaattggatg gtccgctgag ttgtactata gagggattcg gctccgaaca
      781 ttcgagtgtc aaattgagca agatttgaac gaaaagcctg gatttgttgc gggtaaatag
      841 cttccagggt tgcaattttc aaaccttcta acttcccaag ttgccattca cggagattag
      901 gaacgatttc taaagaacag ggggtatgga gttgactttg gataatctca gcagatttga
      961 ccgctcgagg taaatcactt gaataaatct gatcaaaagg aatttccttg agatactggc
     1021 caagtcgttt tagggtttca atggattcag gaagaagagg agaatcaccg ctagcacctt
     1081 gaaaacgacc ttcttggttc cagagggtac gaccgtggcg gacaaagtag agtttcatta
     1141 cttgatgtcc tccaaaatat ctacaaagtc tgcctttaca aagctagcca agtcttgtgg
     1201 cgcgacgata atgctgtgtc cgacttcgcc tgcagagaca atcattcgat ccaaatctag
     1261 agcaatttta tcgataaaaa tgggataatt gtgtttctga cgaattccga caggattatt
     1321 ggctccatga atgtaaccag ttgttttttc taagtccttt tgtggaatca tgctcacttt
     1381 tttattgcca gaaattttag ctagtttctt ttcagacaag tgctgagtga tagggacaat
     1441 tccgataatc ggtccggtct tgtctcccaa aagcgccaag gttttgaaaa tctgatctcg
     1501 ttcataacct tgaggaagct ctccttctag ggcattgatt tgaatcccct gatgagggat
     1561 agctgcttta gataggattt gttccaccaa tgtttttttg attttaactt tttttgccat
     1621 tatttatatt tatcctccaa ttgactcatc caaataccaa gccagattcc cagcgcaaag
     1681 aagaaggcga tgatgacata accgacaagt gaaagtcctg tgtattggat actttcagcg
     1741 tttcctgcat ttggaattaa gatcaaaagg gtacttgata ggacgatacc gatgatgaaa
     1801 tgatagacgc gtgagtggta gttgtttaag gcataatcca tcaattttga aaaaacgatg
     1861 agagttgcac ctgcaccaat tccaatcgga aagaaggttc ccaagaaatc aaaagtttta
     1921 aaaccagtca acataggagc atatagtccc aaaatcaaaa gtaaatttga tgggctgagg
     1981 ccaggaacca agacgccaag ggccaatagt gcgcctgcta ggacgaagtt aagaaagctg
     2041 gcgcttaagg ttccaacgac aaaatttaag gcatagagtc ctaatccaga aatgataaag
     2101 gttgtccata accaagctaa atcaatcttg tctcggtcag attctcgagt tgattctttg
     2161 aggaggctag gaactgtacc gataatagct cccgcaaagc tccataatac aaaaacctga
     2221 taattttcaa gcaggtattc aatcgggtag gaaaataagc cgattcccag aagcataccg
     2281 atggcaactg gaataaagta caaaacattt tctttaaagt ctttaaaggg atgggccaga
     2341 aagccaatca ttcgttcata gattcctaag attgctgcta gaacccctcc ggaaattccc
     2401 ggtaggataa atccaagagc aattacaatc cctttaataa cgcgtgctaa ccatgagagc
     2461 atatatttcc tccaactatt tctatttcaa aaaatcctta ctatattgta tcacaatcag
     2521 acacaaaaag aaaaagcaaa tgataaacaa atgcttttaa ggttttaaaa aaagttttcg
     2581 aaaggtttct tctttatttt ttaagggaga gataacgttg atatctaaat cgtggtcaaa
     2641 gccggcaatt tttcctttag atgtgtattg gtgaatatca taatctaaat cagttttagg
     2701 actgctctcc aaaaatcctg agtctgagcc gtaggacgga atccaaacag aggtaaactt
     2761 gcctgtatca atactgtgtt cttccatgaa gtagacacca acgtagatgc cgatgttttt
     2821 agcacctagt gatgctagtt ttgctcgaaa gttttcgaca ccttcgttca tattagacat
     2881 ggttttgtct tccacgtcaa gccaatagta actagggctg taaggagagg cagcattgta
     2941 gaaaacttcg gcagcctttt ccatttcttg gacacttttt ccagctacat aagcgtagac
     3001 agcaactggg acattccgct tttgaagttc agtgatatga ctcttatagg tcttgtctat
     3061 tccattgata aatgaagcat cattttcttt tgttgtttga gcaccactgt gaacacgaac
     3121 aatagcacct gaaatatttt gtgagagggc atcgtagttg atttcctcag gacgctgcca
     3181 gccagagagg tcaataatcg gtttgtctaa gtgtttcaaa gcctgtgcta tattggattt
     3241 tgttttaaac gattggctgt cattaagtgg gcgattgatg attaaaatga acatcataat
     3301 cccaaaaaaa ctaaataaaa taagtggatg aatttgtttt ctcatatctt ataagtctac
     3361 cctaaaaatc aaaaaaaatc aaaaaaatgg gttaaggaag agactttaga gcattttttc
     3421 attcaagagt gcggaatgat ttgaaatatg gtataataaa agggaatttc tacagaaaag
     3481 agaagattat gtcaaatttt gccattattt tagcagcggg taaagggact cgcatgaaat
     3541 ctgatttgcc aaaagttttg cacaaggttg cgggtatttc tatgttggaa catgttttcc
     3601 gtagtgtggg agctatccaa cctgaaaaga cagtaacagt tgtaggacac aaggcagaat
     3661 tggttgagga ggtcttgact ggacagacag aatttgtgac tcaatctgaa cagttgggaa
     3721 ctggtcatgc agttatgatg acagaaccta tcttagaagg tttgtcagga cacaccttgg
     3781 tcattgcagg agatactcct ttaatcactg gtgaaagctt gaaaaacttg attgatttcc
     3841 atatcaatca taaaaatgtg gccactatct tgactgctga aacggataat ccttttggct
     3901 atggacgaat tgttcgtaat gacaatgctg aggttcttcg tattgttgag cagaaggatg
     3961 ctacagattt tgaaaagcaa atcaaggaaa tcaacactgg aacatacgtc tttgacaacg
     4021 agcgtttgtt tgaggctttg aaaaatatca ataccaataa cgctcaaggc gaatactata
     4081 ttacagacgt cattggtatt ttccgtgaaa ctggtgaaaa agttggcgct tatactttga
     4141 aagattttga tgaaagtctt ggggtaaatg accgtgtggc gcttgcgaca gctgagtcag
     4201 ttatgcgtcg tcgcatcaat cataaacaca tggtcaacgg tgttagcttt gtcaatccag
     4261 aagcaactta tatcgatatt gatgttgaga ttgctccgga agttcaaatc gaagccaatg
     4321 ttaccttgaa agggcaaacg aaaattggtg ctgagactgt tttgacaaat ggtacttatg
     4381 tagtggacag cactatcgga gcaggagcgg tcattaccaa ttctatgatt gaggaaagta
     4441 gtgttgcaga cggtgtgaca gtcggtcctt atgctcacat tcgtccaaat tcaagtctgg
     4501 gtgcccaagt tcatattggt aactttgttg aggtgaaagg atcttcaatc ggtgagaata
     4561 ccaaggctgg tcatttgact tatatcggaa actgtgaagt gggaagcaac gttaatttcg
     4621 gtgctggaac tattacagtc aactatgacg gcaaaaacaa atacaagaca gtcattggag
     4681 acaatgtctt tgttggttca aattcaacca ttattgcacc agtagaactt ggtgacaatt
     4741 ccctcgttgg tgctggttca actattacta aagacgtgcc agcagatgct attgctattg
     4801 gtcgcggtcg tcagatcaat aaagacgaat atgcaacacg tcttcctcat catcctaaga
     4861 accagtagga gcctatcatg gagtttgaag aaaaaacgct tagccgaaaa gaaatctatc
     4921 aaggaccaat atttaaactg gtccaagatc aggttgaatt accagaaggc aagggaactg
     4981 cccaacggga tttgattttc cacaatgggg ctgtctgtgt tttagcagta acggatgaac
     5041 aaaaacttat cttggtcaag cagtaccgca aagctatcga ggcagtctct tacgaaattc
     5101 cagccggaaa attggaagta ggagaaaaca cagcccctgt ggcagctgcc cttcgtgaat
     5161 tagaggaaga aacagcctat acagggaaat tagaactctt gtacgatttt tattcagcta
     5221 ttggcttttg taatgagaag ttaaaactat atttagcaag cgatttaaca aaagtggaaa
     5281 atccgcgtcc gcaggatgag gatgaaacct tggaagtcct tgaagtgagc ttagaagaag
     5341 cgaaagaatt aatccaatca ggtcatattt gtgatgccaa gacaattatg gctgttcagt
     5401 attgggagtt gcagaaaaaa tagaggaggt cagtatgggt aaatctttat taacggatga
     5461 aatgattgaa agagctaata gaggcgaaaa aatttcaggt cctcctttgc tagatgataa
     5521 tgaggaaact aagattttac caacctcttc ttcccgtttt ggttatgcca atcctaagga
     5581 tcatggtttt agccaggaaa ccttgaagat tcaggtcgaa ccatctattc ataaaagccg
     5641 tcgtattgaa aataccaaga gaaatgtctt caattctaag ttgaataaaa tcttatttgc
     5701 ggtcattttt ctcttgattt tgcttgtttt agcaatgaaa cttttgtaat agaaaaggaa
     5761 ttgaaatgaa aataggaatt attgctgcta tgccagaaga actggcttat ctggtccagc
     5821 atttagataa tgcccaggag caagttgttt tggggaatac ctatcataca ggaaccattg
     5881 cttctcatga agtcgttctt gtagaaagtg gaattggtaa ggtcatgtct gctatgagtg
     5941 tggcgatttt ggctgatcat ttccaggtgg atgcccttat taatacgggt tcagctgggg
     6001 cagtagcaga aggtatcgct gttggggatg tcgtgattgc tgacaaatta gcctatcatg
     6061 acgtggatgt cacagctttt ggctattctt atggacaaat ggcgcaacaa ccgctttatt
     6121 tcgaatcaga caaaaccttt gttgctcaaa tccaagagag tttatctcaa ttggaccaaa
     6181 actggcattt tggtttgatt gctacaggag atagttttgt tgcaggaaat gacaagatag
     6241 aagcgattaa gtcccatttc ccagaagttt tagccgtgga gatggagggg gcagctattg
     6301 ctcaagcagc gcatgccctc aatctcccag tcttagtcat cagagctatg agtgacaatg
     6361 ccaaccatga agcaaacatc ttttttgatg agtttattat cgaagctgga cgtcgctctg
     6421 cccaagtctt gttgaccttt ttgaaggctt tagattaagc ggaaatttga cagtttttct
     6481 agcttatgat aagatttaag taaagaaaag ctagaaaacg tttcagagga tattatgagt
     6541 attgaaatga ccgtcagtga gattgcagag gtcttaggat tatctcgcca agcaatcaat
     6601 aaccgtgtca aagaattacc agaagaagac acagataaaa atgacaaagg ggtaacagtt
     6661 gttaccagaa gtggcttgat taagctagaa gaaatctata aaaaaacgat ttttgaagat
     6721 gagcctgtca gtgaagatgt caaacaacgt gaactgatgg agattctagt ggatgagaag
     6781 aatgcagaaa ttcttcgtct ttatgaacaa ttaaaagcca aggatcgtca gttatcagaa
     6841 aaagacgagc agatgcgtat caaagaccgt cagattgctg agaaagacaa acaattagat
     6901 cagcaacaac aattgaccct tcaagccatg aaggatcaag aaaatcttaa gctagagctg
     6961 gaccaagcaa aagaagaagt ccaatccact aaaaaaggct tttttgctcg tttatttgga
     7021 ggataatcaa ggagctgttt aggttaaatg ctaacctgat ggcttctttt gtttgtgaat
     7081 agtatagttg attagaacag agacagtaga ggagaggtac agaatggaac gattagaaga
     7141 ttacattgct tttgacttag aatttaatca gcacgaagga caaaaacact tgattcaagt
     7201 atcggcagtg cgttttagag atggtcggga aatcgatgcc tatgattcct atgttcatac
     7261 cagcgtccct ctaaaaagct ttatcaatgg tttgaccggg attacagctg agaccttaaa
     7321 agatgctcct ccagttgaga aagtcttaga agaattccaa gagtttgtgg gttctttgct
     7381 gatgataggc tacaatgctg ctaaaagtga tttacctatt ttagcagagt atggtttaga
     7441 ctatagccaa cagtacaagg tggatttgta tgatgaagct tttgaacgtc ggagttcaga
     7501 tttacatggt attgccaatc tcaaattgca aactgttgcc tcatttcttg gatttcaagg
     7561 tcagtctcat aatagtttag aggatgctcg gatgacggcg cgtgtttacg aatccttctt
     7621 agagtcggat caagctaggg aattgttaga gacagaaagt agcttatcaa acaatccttt
     7681 tggtggcttg gacttgtctc aattatttga ctaggagtgc ctatgaaacc tgaaaaacct
     7741 aaaaaattgg gtcttaaaca gatttacctc aatcttgata aaatcctctt tctatttttc
     7801 ttgattttta tgatagtgga gtatttgtgg gtaccattca attcatggtt ggctagcttc
     7861 ttattagatc agacaggtta tctttttatt tcctataaca atgtcttagc catccttact
     7921 cattctccat tgattagtct agctttttta gccttgattg taattaacct tctggttgct
     7981 tattttcaaa taggactcct ctttattggt gctcgtcatc tcctctataa tgaaaagcga
     8041 aggctaattg agtatatccg caaggttttc catgaaagtt ttggatttat gaaaaagcta
     8101 acttgcgcta aagccttgtt tatctttttc tatatagcta tgctttttcc ttttatacgg
     8161 aaaatgctga agatttatta tcttaataaa atcgtcattc cagagtttat ccaagcttat
     8221 ttagaagata aatattggat gtggtggctg agcatcctac ttctatctct tatctttctc
     8281 tatgtctctg tcagattgat gtttgccctt ccgaagattg tatatgatca gctgactgtt
     8341 cgagaagcag tgatgtttag cttggagaaa accaagaaga gagttacttt ttatgcttgg
     8401 aatttatttt acatcttgct caaagcacat ttattatttt atcttccttt gattccttta
     8461 ttattggctc aaactttggt agatgatatc actcagagag agtctctgat tcttggtatt
     8521 ttgaattttg tcgtgattaa aaatctatat tatatggctc tgacttattt tttggttaaa
     8581 tttgtttcat ttttgacagg aaaggagctg gatatgcttc ctaggagaga aaaagatcac
     8641 atcgtgcgat ggggtgtcat gacttgtgcc agtcttttct ttgccttaga aggttatatt
     8701 tatctggagg ctcccatggt tcatctacct caacttattt ctcaccgagg ggtttccaat
     8761 gcaaatggga ttcaaaatac agtagagtcc ttggaaacta cagcacaact caaaccagac
     8821 ttggtggaga cggacgtgca ggaaacaaaa gatgggcagt ttgtcatgat gcatgatgct
     8881 aacttgaaaa atctagcagg tatcaataaa agtcctcaag acttaaactt ggaggagctt
     8941 aaagggattg atatttttga aaatggctac cagactaaaa tttcaagctt tgaagattat
     9001 ctcagtcgag ccaacgaact tggtcaaaaa ttactaattg aaattaaaac cagtaaaaaa
     9061 gatagtccag acatgatgaa ccgcttttta gcccgttatg ctgcaaagct caagatttat
     9121 ggacatcaaa tccagtcttt agactaccat gttgtcgaaa aagtaagaca gtatgatgcc
     9181 gaactgccag tttatttcat catgccctac aattctgtct ttcctaaaac aagagcgaca
     9241 ggatatacta tggagtactc aaccttggat gaatattttg taagcaaact atggacaacg
     9301 gatcagaaac tttatgtctg gactgtcaat gattcagaag ctatcagcaa atctcttcac
     9361 ttaggagtag atggagtgat tacagatgat ttggaaaaag ttcagcggga aatagaagta
     9421 gcccaagaag atccagaata tacggatttg ctcttgaaaa aagcattgga attctttgaa
     9481 ttttagtaat aaaaaagagg aacggttgtt cctcccatga taggtaagta ttcttgtgaa
     9541 tgcttgcttt tt
//
LOCUS       DAVAAL010000065         9551 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVAAL010000065 DAVAAL010000000
VERSION     DAVAAL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.066"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            145..5085
                     /locus_tag="VQM60_001320"
     CDS             145..5085
                     /locus_tag="VQM60_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET1081184.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            5175..6371
                     /locus_tag="VQM60_001321"
     CDS             5175..6371
                     /locus_tag="VQM60_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET1081185.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            6507..7034
                     /locus_tag="VQM60_001322"
     CDS             6507..7034
                     /locus_tag="VQM60_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET1081186.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7111..7467
                     /gene="glnR"
                     /locus_tag="VQM60_001323"
     CDS             7111..7467
                     /gene="glnR"
                     /locus_tag="VQM60_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET1081187.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7504..8850
                     /gene="glnA"
                     /locus_tag="VQM60_001324"
     CDS             7504..8850
                     /gene="glnA"
                     /locus_tag="VQM60_001324"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET1081188.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8946..9065)
                     /locus_tag="VQM60_001325"
     CDS             complement(8946..9065)
                     /locus_tag="VQM60_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET1081189.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
BASE COUNT         3079 a         1740 c         2087 g         2645 t
ORIGIN      
        1 tttagatttg tgaaaacagc tattgttttt tgtctaaaaa tgttttagga taaaaatgtg
       61 attatcatga ttctagagtt aaaaaactac agaatataaa gcgctttctt gaaaacaaca
      121 aaatcaatct tttaggagga gaaaatgaag aatccatttt ttgaaagacg ttgtcgttac
      181 agtattcgta agttatcagt aggagcctgc tcgctgatga ttggtgctgt tttatttgct
      241 ggtccagcct tggctgaaga aactgcagtt cctgaaaata gcggagctaa tacagagctt
      301 gtttcaggag agagtgagca ttcgaccaat gaagctgata agcagaatga aggggaacat
      361 actagagaaa acaagctaga aaaggcagaa ggagtagcga cagcatctga aactgcagaa
      421 gcagctagcg cagctaaacc agaggaaaaa gcaagtgagg tggttgcaga aacaccatct
      481 gcagaagcaa aacctaagtc tgacaaggaa acagaagcaa agcccgaagc aactaaccaa
      541 ggggatgagt ctaagccagc agccgaagct aataagactg aaaaagaagt ccagccagat
      601 gtccctaaaa atacagaaaa aacattaaaa ccaaaggaaa tcaaatttaa ttcttgggaa
      661 gaattgttaa aatgggaacc aggtgctcgt gaagatgatg ctattaaccg cggatctgtt
      721 gtcctcgctt cacgtcggac aggtcattta gtcaatgaaa aagctagcaa ggaagcaaaa
      781 gttcaagcct tatcaaacac caattctaaa gcaaaagacc atgcttctgt tggtggagaa
      841 gagttcaagg cctatgcttt tgactattgg caatatctag attcaatggt cttctgggaa
      901 ggtctcgtac caactcctga cgttattgat gcaggtcacc gtaacggggt tcctgtatac
      961 ggtacactct tcttcaactg gtctaatagt attgcagatc aagaaagatt tgctgaagct
     1021 ttgaagcaag acgcagatgg tagcttccca attgcccgta aattggtaga catggccaag
     1081 tattatggct atgatggcta tttcatcaac caagaaacaa ctggagattt ggttaaacct
     1141 cttggagaaa agatgcgcca gtttatgctc tatagcaagg aatatgctgc taaggtaaac
     1201 catccaatca agtattcttg gtacgatgcc atgacctata actatggacg ttaccatcaa
     1261 gatggtttgg gagaatacaa ctaccaattc atgcaaccag aaggagataa ggttccggca
     1321 gataacttct ttgctaactt taactgggat aaggctaaaa atgattacac tattgcaact
     1381 gccaactgga ttggtcgtaa tccttatgat gtatttgcag gtttggaatt gcaacagggt
     1441 ggttcctaca agacaaaggt taagtggaat gacattttag acgaaaatgg gaaattgcgc
     1501 ctttctcttg gtttatttgc cccagatacc attacaagtt taggaaaaac tggtgaagat
     1561 tatcataaaa atgaagatat cttctttaca ggttatcaag gagaccccac tggccaaaaa
     1621 ccaggtgaca aagattggta tggtattgct aacctagttg cggaccgtac gccagcggta
     1681 ggtaatactt ttactacttc ttttaataca ggtcatggta aaaaatggtt cgtagatggt
     1741 aaggtttcta aggattctga gtggaattat cgttcagtat caggtgttct tccaacatgg
     1801 cgctggtggc agacttcaac aggggaaaaa cttcgtgcag aatatgattt tacagatgcc
     1861 tataatggcg gaaattccct taaattctct ggtgatgtag ccggtaagac agatcaggat
     1921 gtgagacttt attctactaa gttagaagta actgagaaga ccaaacttcg tgttgcccac
     1981 aagggaggaa aaggttctaa agtttatatg gcattctcta caactccaga ctacaaattc
     2041 gatgatgcag atgcatggaa agagctaacc ctttctgaca actggacaaa tgaagaattt
     2101 gatcttagct cactagcggg taaaaccatc tatgcagtca aactattttt cgagcatgaa
     2161 ggtgctgtaa aagattatca gtttaaccta ggacaattaa ctatctcgga caatcaccaa
     2221 gagccacaat cgccgacaag cttttctgta gtgaaacaat ctcttaaaaa tgcccaagaa
     2281 gcggaagcag ttgtgcaatt taaaggcaac aaggatgcag atttctatga agtttatgaa
     2341 aaagatggag acagctggaa attactaact ggctcatctt ctacaactat ttatctacca
     2401 aaagttagcc gctcagcaag tgctcagggt acaactcaag aactgaaggt tgtagcagtc
     2461 ggtaaaaatg gagttcgttc agaagctgca accacaacct ttgattgggg tatgactgta
     2521 aaagatacca gcctaccaaa accactagct gaaaatatcg ttccaggtgc aacagttatt
     2581 gatagtactt tccctaagac tgaaggtgga gaaggtattg aaggtatgtt gaacggtacc
     2641 attactagct tgtcagataa atggtcttca gctcagttga gtggtagtgt ggatattcgt
     2701 ttgaccaagc cacgtaccgt tgttagatgg gtcatggatc atgcaggagc tggtggtgag
     2761 tctgttaacg atggcttgat gaacactaaa gactttgacc tttattataa agatgcagat
     2821 ggtgagtgga agctagctaa ggaagtccgt ggtaacaaag cacacgtgac agatatcact
     2881 cttgataaac caatcactgc tcaagactgg cgcttgaatg ttgtcacttc tgacaatgga
     2941 actccatgga aggctattcg tatctataac tggaaaatgt atgaaaagct tgatactgag
     3001 agtgtcaata ttccgatggc caaggctgca gcccgttctc taggcaataa caaggtacaa
     3061 gttggctttg cagatgtacc ggctggagca actattaccg tttatgataa tccaaattct
     3121 caaactccgc tcgcaacctt gaagagcgaa gttggaggag acctagcaag tgcaccattg
     3181 gatttgacaa atcaatctgg tcttctttat tatcgtaccc agttgccagg caaggaaatt
     3241 agtaatgtcc tagcagtttc cgttccaaaa gatgacagaa gaatcaagtc agtcagccta
     3301 gaaacaggac ctaagaaaac aagctacgcc gaaggggagg atttggacct tagaggtggt
     3361 gttcttcgag ttcagtatga aggaggaact gaggacgaac tcattcgcct aactcacgca
     3421 ggtgtatcag tatcaggttt tgatacgcat cataagggag aacagaatct tactctccaa
     3481 tatttgggac aaccagtaaa tgctaatttg tcagtgactg tcactggcca agatgaagca
     3541 agtccgaaaa ctattttggg aattgaagta agtcaggaac cgaaaaaaga ttacctagtt
     3601 ggtgatagct tagacttgtc tgaaggacgc tttgcagtgg cttatagcaa tgacaccatg
     3661 gaagaacatt cctttactga tgagggagtt gaaatttctg gttacgatgc tcaaaagact
     3721 ggtcgtcaaa ccttgacgct tcgttaccaa ggtcatgaag tcaactttga tgttttggta
     3781 tctccaaaag cagcattgaa cgatgagtac ctcaaacaaa aattagcaga agttgaagct
     3841 gctaagaaca aggtggtcta taactttgct tcaccagaag taaaagaagc tttcttgaaa
     3901 gcaattgaag cggccgaaca agtgttgaaa gaccatgaaa ctagcaccca agatcaagtc
     3961 aatgaccgac ttaataaatt gacagaagct cataaagctc tgaatggtca agagaaattt
     4021 aaggaagaaa agacagagct tgatcgttta acaggtgagg ttcaagaact cttggctgcc
     4081 aaaccaaacc atccttcagg ttctgcccta gctccgcttc ttgagaaaaa caaggccttg
     4141 gttgaaaaag tagatttgag tccagaagag cttgcaacag cgaaacagag tctaaaagat
     4201 ctggttgctt tattgaaaga agacaagcca gcagtctttt ctgatagtaa aacaggtgtt
     4261 gaagtacact tctcaaataa agagaagact gtcatcaagg gtttgaaagt agagcgtgtt
     4321 caagcaagtg ctgaagagaa gaaatacttt gctggagaag atgctcatgt ctttgaaata
     4381 gaaggtttgg atgaaaaagg tcaagatgtt gatctctctt acgcttctat tgtgaaaatc
     4441 ccaattgaaa aagataagaa agttaagaaa gtatttttct tacctgaagg caaagaggca
     4501 gtagaattgg cttttgaaca aacggatagt catgttatct ttacagcacc acactttact
     4561 cattatgcct ttgtttatga atctgctgaa aaaccacaac ctgctaaacc agcaccacaa
     4621 aacaaagtcc ttccaaaacc tacttatcaa ccggcttctg atcaacaaaa ggctcctaaa
     4681 ttggaagttc aagaggaaaa ggttgccttt catcgtcaag agcatgaaaa tactgagatg
     4741 ctagttgggg aacaacgagt catcatacag ggacgagatg gactgttaag acatgtcttt
     4801 gaagttgatg aaaacggtca gcgtcgtctt cgttcaacag aagtcatcca agaagcgatt
     4861 ccagaaattg ttgaaattgg aacaaaagta aaaacagtac cagcagtagt agctacacag
     4921 gaaaaaccag ctcaaaatac agcagttaaa tcagaagaag caagcaaaca attgccaaat
     4981 acaggaacag ctgatgctaa tgaagcccta atagcaggct tagccagcct tggtcttgct
     5041 agtttagcct tgaccttgag acggaaaaga gaagataaag attaaatatc gaaaaatctt
     5101 gtgaaatctt tccttatatt tccaaagtgt gatataatag tttcgaataa aataaataaa
     5161 ggagtttttg taacatggca aaacttactg ttaaagacgt tgacttgaaa ggtaaaaaag
     5221 tcctcgttcg tgttgacttc aacgtaccat tgaaagatgg cgtaatcact aacgataacc
     5281 gtatcacagc agctcttcca actattaagt acatcatcga acaaggtgga cgtgcaattc
     5341 ttttctctca ccttggacgt gtgaaagaag aagctgataa agctggtaaa tcacttgctc
     5401 ctgtagcagc tgacttggca gcaaaacttg gtcaagatgt tgttttccca ggtgtcactc
     5461 gtggtgctga attggaagcg gcaatcaacg ctcttgaaga tggacaagtt ctcttggttg
     5521 aaaacactcg ttacgaagat gttgacggca agaaagaatc taaaaacgat cctgaacttg
     5581 gtaaatactg ggcatcactt ggagatggta tcttcgtaaa cgatgcattc ggtacagctc
     5641 accgtgcaca cgcatctaac gttggtatct cagcaaacgt tgaaaaagca gttgctggtt
     5701 tccttcttga aaacgaaatt gcctacatcc aagaagcagt tgaaactcca gaacgtccat
     5761 tcgtggctat ccttggtggt tcaaaagttt cagacaagat cggtgttatc gaaaacttgc
     5821 ttgaaaaagc tgataaagtc cttatcggtg gtgggatgac ttacacattc tacaaagcac
     5881 aaggtatcga aatcggtaac tcacttgtag aagaagacaa attggatgtt gcgaaagctc
     5941 ttcttgaaaa agcaaatggt aaattgatct tgccagttga ctcaaaagaa gctaacgcat
     6001 ttgctggtta cactgaagtg cgtgacactg aaggtgaagc agtttctgaa ggcttccttg
     6061 gtcttgacat cggtccaaaa tctatcgcca aatttgacga agctttgact ggtgccaaaa
     6121 cagttgtatg gaacggacct atgggtgtat ttgaaaaccc agatttccaa gctggtacaa
     6181 tcggtgtgat ggacgctatc gtgaaacaac caggagttaa atcaatcatc ggtggtggtg
     6241 actcagctgc cgcagcgatt aaccttggcc gtgcagacaa gttctcatgg attagtacgg
     6301 gtggtggagc atcaatggaa cttcttgaag gtaaggttct tccaggactt gcagccttga
     6361 cagaaaaata agattttata aacaaatcaa agaagagagg gatgaaagtt cctcttttct
     6421 tttgcttaaa ataaaaacgc ttcctctcaa ctattactca taaaaatcac cgatttatga
     6481 taaaatggaa atagaaagtt gagattatga gttattttaa aaaatataaa ttcgataaat
     6541 cccagttcaa acttggtatg cgaaccttta aaacaggtat tgctgttttt ctagttctct
     6601 tgatttttgg cttttttggc tggaaaggtc ttcaaattgg tgctttgaca gccgttttta
     6661 gcctgaggga gagttttgat gagagtgttc attttgggac ttcgcgtatt ctaggaaata
     6721 gtatcggtgg actctatgcc ttggtcttct tcttattaaa tacttttttc cacgaagcct
     6781 tttgggtgac cttggtagtt gttccaatct gcaccatgtt aaccattatg acaaatgtag
     6841 ccatgaataa caaagcaggg gttattggtg gtgtagcagc tatgttaatc attaccctat
     6901 caattccaag tggtgagaca attttgtacg tgtttgtgcg tgtattagaa acgtttatgg
     6961 gagtttttgt cgcaattatc gtaaattacg atattgatcg tattcgtctc tttttagaga
     7021 aaaaagaaaa ataatgttac attttataac attatcaatt gacgtttgtc tttttttaga
     7081 ctataataga cagaaagaag gaaattgtaa atgaaggaaa aagaatttcg ccgaaatatg
     7141 gctgtttttc ctatcggcag tgttatgaag ttgaccgatt tatcggcgcg tcagattcgt
     7201 tattatgaag atcaagagtt gatcaagccc gatcgaaacg aaggaaatcg tcgcatgtat
     7261 tccttgaatg acatggatcg tctgcttgaa atcaaagatt atatctctga aggttataat
     7321 atcgctgcca ttaagaaaaa atatgctgaa cgtgaagcga aatccaagaa agcggttagt
     7381 cagactgaag tacgtcgtgc acttcacaat gaactcctcc aacaggggcg ttttgcttca
     7441 gtacagtcac cttttggtcg cggttaggca accgcaagta gtcatagatt aaggagaaaa
     7501 cttatgccaa tcacagctgc agatattcgt cgtgaagtca aggaaaaaaa tgttaccttt
     7561 attcgtctta tgttctcaga tattttggga accatgaaaa acgtcgaaat tcctgctaca
     7621 gatgaacagt tagataaggt cttgtcgaac aaggttatgt ttgatggatc ttctattgaa
     7681 ggttttgtac gtatcaatga gtcggatatg tacttgtacc cggacttgga tacatggaca
     7741 gtcttccctt ggggagatga aaatggaagt gttgcaggtc tgatctgtga tgtttataca
     7801 acagaaggtg aaccatttgc gggtgaccct cgtggtaatt tgaaacgagc tcttcgtcac
     7861 atggaagaag ttggattcaa atccttcaac cttggtccag agccagaatt cttcctattt
     7921 aagttggatg aaaatgggga cccaacactt gaagtgaatg acaagggtgg ctactttgat
     7981 ttggcaccta ctgaccttgc ggacaacaca cgtcgtgaga ttgtgaatgt cttgaccaaa
     8041 atgggatttg aagtagaagc gagtcaccac gaggttgcgg ttggacagca tgagattgac
     8101 tttaagtacg atgaagttct ccgtgcttgt gataagattc aaatctttaa acttgttgtt
     8161 aaaaccattg ctcgcaaaca cggactttac gcaacattta tggcgaagcc aaaatttggt
     8221 attgctggct caggtatgca ctgtaatatg tccttgtttg atgcagaagg aaataacgcc
     8281 ttctttgatc caaatgatcc aaaaggaatg cagttgtcag aaacagctta ccatttccta
     8341 ggcggtttga tcaagcatgc ttacaactat actgccatca tgaacccaac agttaactca
     8401 tacaaacgtt tggttccagg ttatgaagcg cctgtttaca ttgcttgggc tggtcgtaac
     8461 cgttcgccac ttgtgcgcgt acctgcttca cgtggtatgg gaactcgtct tgagttgcgt
     8521 tcagtggatc caatggcgaa cccttacgtt gctatggctg ttcttttgga agttggtttg
     8581 tatggtattg aaaataaaat cgaagcacca gctcctatcg aagaaaatat ctacatcatg
     8641 acagcagaag agcgcaagga agctggtatt acagaccttc catcaactct tcacaacgct
     8701 ttgaaagctt tgacagaaga tgaagtggtt aaagctgctc tcggagatca catctacact
     8761 agcttccttg aagccaaacg aatcgaatgg gcaagttatg caaccttcgt ttcacaatgg
     8821 gaaattgata attatttaga cctttactaa tactaatata gaagaaagat tgcctgtggg
     8881 taaacagtca cacagcagtc taagccatca aatacctata acccttaaaa tagcgatttc
     8941 ggtcgctatt tatttttcga aaaatcctct tgactctttt gcgaattaga tttatcatca
     9001 gacgtgtaga agaaaatcaa ggagtcacta tggaaaaatt caatttcaaa aacaatatcg
     9061 ggcaagaaaa caagttactt caaatcgaaa tctacaagtt tactaacttt tacaagcttc
     9121 aaaattacac atctgtaaat attttttcga aagatatatt tgaagccata gtaaattgag
     9181 actagaatag tacactacat ctgctaaaaa tttctagaaa ctaatttaac tttcctaatc
     9241 gatttgttta catcttattt caatctacta tattatctca ataaatgatg atggcgactg
     9301 ttttgtttgt gcttgtcgat ttttgaatcc ctgatcgagc ggaagaatat ttctgccgag
     9361 gttgccaagg ggaaaatact gaagatattg aattttatga tgaacaatta caggcagaaa
     9421 aggtggaggt tttgaatatt cgattggaag taaaaaattg aattatctac aatctcatca
     9481 acaataaacc tatttcaacc ccattatcta taggcgtatt tttacgcctt tttatatact
     9541 tgaagcacat a
//
LOCUS       DAVAAL010000066         9524 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVAAL010000066 DAVAAL010000000
VERSION     DAVAAL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.067"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            334..2994
                     /gene="rrgA"
                     /locus_tag="VQM60_001326"
     CDS             334..2994
                     /gene="rrgA"
                     /locus_tag="VQM60_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HET1081190.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYERKDKSVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDPENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNAFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAVGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GAPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVFYDTTEKRIRVTGLYLGTGEKVTLTYNVRLNDQFVSN
                     KFYDTNGRTTLHPKEVEKNTVRDFPIPKIRDVRKYPAITIAKEKKLGEIEFIKINKND
                     KKPLRDAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLPFYLIGCMMMGGVLLYTRKHP"
     gene            3217..5214
                     /gene="rrgB"
                     /locus_tag="VQM60_001327"
     CDS             3217..5214
                     /gene="rrgB"
                     /locus_tag="VQM60_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus major pilin RrgB"
                     /protein_id="HET1081191.1"
                     /translation="MKSINKFLTMLAALLLTASSLFSAATVFAAGTTTTSVTVHKLLA
                     TDGDMDKIANELETGNYAGNKVGVLPANAKEIAGVMFVWTNTNNEIIDENGQTLGVNI
                     DPQTFKLSGAMPATAMKKLTEAEGAKFNTANLPAAKYKIYEIHSLSTYVGEDGATLTG
                     SKAVPIEIELPLNDVVDAHVYPKNTEAKPKIDKDFKGKANPDTPRVDKDTPVNHQVGD
                     VVEYEIVTKIPALANYATANWSDRMTEGLAFNKGTVKVTVDDVALEAGDYALTEVATG
                     FDLKLTDAGLAKVNDQNAEKTVKITYSATLNDKAIVEVPESNDVTFNYGNNPDHGNTP
                     KPNKPNENGDLTLTKTWVDATGAPIPAGAEATFDLVNAQTGKVVQTVTLTTDKNTVTV
                     NGLDKNTEYKFVERSIKGYSADYQEITTAGEIAVKNWKDENPKPLDPTEPKVVTYGKK
                     FVKVNDKDNRLAGAEFVIANADNAGQYLARKADKVSQEEKQLVVTTKDALDRAVAAYN
                     ALTAQQQTQQEKEKVDKAQAAYNAAVIAANNAFEWVADKDNENVVKLVSDAQGRFEIT
                     GLLAGTYYLEETKQPAGYALLTSRQKFEVTATSYSATGQGIEYTAGSGKDDATKVVNK
                     KITIPQTGGIGTIIFAVAGAVIMGIAVYAYVKNNKDEDQLA"
     gene            5261..6442
                     /gene="rrgC"
                     /locus_tag="VQM60_001328"
     CDS             5261..6442
                     /gene="rrgC"
                     /locus_tag="VQM60_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HET1081192.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDNRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKADTVTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDGSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIVVTNLPLGTY
                     RFKEVEPLAGYTVTTMDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEENGHYTPVLQNGKEVVVASGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKTN
                     N"
     gene            6781..7677
                     /gene="srtC-1"
                     /locus_tag="VQM60_001329"
     CDS             6781..7677
                     /gene="srtC-1"
                     /locus_tag="VQM60_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HET1081193.1"
                     /translation="MAKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPAIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     RQSERALKALKEATKEVKVEDE"
     gene            7670..8563
                     /gene="srtC-2"
                     /locus_tag="VQM60_001330"
     CDS             7670..8563
                     /gene="srtC-2"
                     /locus_tag="VQM60_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HET1081194.1"
                     /translation="MSKSRYSRKKSVKKKKNPFILLLIFLVGLAVAMYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTDQEKKQGVSEYAN
                     MLKVHERIGYVEIPAIEQEIPMYVGTSEDILQKGAGLLEGASLPVGGENTHTVITAHR
                     GLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGQDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLGAMAVILLLLYRV
                     YRNRRIVKGLEKQLEGRHVKD"
     gene            8550..9401
                     /gene="srtC-3"
                     /locus_tag="VQM60_001331"
     CDS             8550..9401
                     /gene="srtC-3"
                     /locus_tag="VQM60_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-3"
                     /protein_id="HET1081195.1"
                     /translation="MSRTKLRALLGYLLMLVACLIPIYCFGQMVLQSLGQVKGHATFV
                     KSMTTEMYQEQQNHSLAYNQRLASQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLGMGLAHVDGTPLPLDGTGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTFNKRLLV
                     NFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWLYRGLVVLAFLGILFVLWKL
                     ARLLRGK"
BASE COUNT         3089 a         1562 c         2214 g         2659 t
ORIGIN      
        1 aaatattttt ttatcaaata ttgtcgttct ataaaaaata tgtgataaaa atatctattg
       61 tgatggaagt tgttttaatt tatactagga tagttaatag taatactata ctatactata
      121 ttgtatacaa gtgtgtcatt gccaggttga gaagatagct ataacgcact tttatacgct
      181 tttgctacgt ttgttagtga acggattaac tcagtgagat aaattttatc agaacataag
      241 taatccgttt cttcgtgtat acagattgaa agtacctatg aatcatagaa ggattaactt
      301 gttctatgaa taatgcttaa cagggagaca cacatgaaaa aagtaagaaa gatatttcag
      361 aaggcagttg caggactgtg ctgtatatct cagttgacag ctttttcttc gatagttgct
      421 ttagcagaaa cgcctgaaac cagtccagcg ataggaaaag tagtgattaa ggagacaggc
      481 gaaggaggag cgcttctagg agatgccgtc tttgagttga aaaacaatac ggatggcaca
      541 actgtttcgc aaaggacaga ggcgcaaaca ggagaagcga tattttcaaa cataaaacct
      601 gggacataca ccttgacaga agcccaacct ccagttggtt ataaaccctc tactaaacaa
      661 tggactgttg aagttgagaa gaatggtcgg acgactgtcc aaggtgaaca ggtagaaaat
      721 cgagaagagg ctctatctga ccagtatcca caaacaggga cttatccaga tgttcaaaca
      781 ccttatcaga ttattaaggt agatggttcg gaaaaaaacg gacagcacaa ggcgttgaat
      841 ccgaatccat atgaacgtgt gattccagaa ggtacacttt caaagagaat ttatcaagtg
      901 aataatttgg atgataacca atatggaatc gaattgacgg ttagtgggaa aacagtgtat
      961 gaacgaaaag ataagtctgt gccgctggat gtcgttatct tgctcgataa ctcaaatagt
     1021 atgagtaaca ttcgaaacaa gaatgctcga cgtgcggaaa gagctggtga ggcgacacgt
     1081 tctcttattg ataaaattac atctgatcca gaaaataggg tagcgcttgt gacttatgct
     1141 tccactatct ttgatgggac cgagtttaca gtagaaaaag gggtagcaga taaaaacgga
     1201 aaacgattga atgattctct tttttggaat tatgatcaga cgagttttac aaccaatacc
     1261 aaagattata gttatttaaa gctgactaat gataagaatg acattgtaga attaaaaaat
     1321 aaggtaccta ccgaggcaga agatcatgat ggaaatagat tgatgtacca attcggtgcc
     1381 acttttactc agaaagcttt gatgaaggca gatgagattt tgacacaaca agcgagacaa
     1441 aatagtcaaa aagtcatttt ccatattacg gatggtgtcc caactatgtc gtatccgatt
     1501 aattttaatc atgctacgtt tgctccatca tatcaaaatc aactaaatgc attttttagt
     1561 aaatctccta ataaagatgg aatactatta agtgatttta ttacgcaagc aactagtgga
     1621 gaacatacaa ttgtacgcgg agatgggcaa agttaccaga tgtttacaga taagacagtt
     1681 tatgaaaaag gtgctcctgc agctttccca gttaaacctg aaaaatattc tgaaatgaag
     1741 gcggttggtt atgcagttat aggcgatcca attaatggtg gatatatttg gcttaattgg
     1801 agagagagta ttctggctta tccgtttaat tctaatactg ctaaaattac caatcatggt
     1861 gcccctacaa gatggtacta taacgggaat attgctcctg atgggtatga tgtctttacg
     1921 gtaggtattg gtattaacgg agatcctggt acggatgaag caacggctac tagttttatg
     1981 caaagtattt ctagtaaacc tgaaaactat accaatgtta ctgacacgac aaaaatattg
     2041 gaacagttga atcgttattt ccacaccatc gtaactgaaa agaaatcaat tgagaatggt
     2101 acgattacag atccgatggg tgagttaatt gatttgcaat tgggcacaga tggaagattt
     2161 gatccagcag attacacttt aactgcaaac gatggtagtc gcttggagaa tggacaagct
     2221 gtaggtggtc cacaaaatga tggtggcttg ctaaaaaatg caaaagtgtt ctatgatacg
     2281 actgagaaaa ggattcgtgt aacaggtttg taccttggaa cgggtgaaaa agttacattg
     2341 acttataatg ttcgcttgaa tgaccaattt gtaagcaata aattctatga cacgaatggt
     2401 cgaacaaccc tacaccctaa ggaagtagaa aagaacacag tgcgcgactt cccgattcct
     2461 aagattcgtg atgtgcgaaa atatccagca attacgattg caaaagagaa aaaacttggt
     2521 gaaattgagt ttattaagat caataagaat gataaaaaac cactgagaga tgcggtcttt
     2581 agtcttcaaa aacaacatcc ggattatcca gatatttatg gagctattga tcaaaatggc
     2641 acttatcaaa atgtgagaac aggtgaagat ggtaagttga cctttaaaaa tctgtcagat
     2701 gggaaatatc gattatttga aaattctgaa ccagctggtt ataaacccgt tcaaaataag
     2761 cctatcgttg ccttccaaat agtaaatgga gaagtcagag atgtgacttc aatcgttcca
     2821 caagatatac cagcgggtta cgagtttacg aatgataagc actatattac caatgaacct
     2881 attcctccaa agagagaata tcctcgaact ggtggtatcg gaatgttgcc attctatctg
     2941 ataggttgca tgatgatggg aggagttcta ttatacacac ggaaacatcc gtaaagtgta
     3001 gaaatgataa tatctatgtt ctgaacaata cttttaagaa gtagcactca agaagagatt
     3061 taagtttact tggtgaaacc agttttcttc gccaagtaaa ccaccattga aaggggagat
     3121 gttttcgaaa acttgcacag aaaaaggatt attattgtca tgtgtaattc attacattgc
     3181 tcacagttga ttttaagaga taaataagga gaaatcatga aatcaatcaa caaattttta
     3241 acaatgcttg ctgccttatt attgacagcg agtagcctgt tttcagctgc aacagttttt
     3301 gcggctggga cgacaacaac atctgttacc gttcataaac tattggcaac agatggggat
     3361 atggataaaa ttgcaaatga gttagaaaca ggtaactatg ctggtaataa agtgggtgtt
     3421 ctacctgcaa atgcaaaaga aattgccggt gttatgttcg tttggacaaa tactaataat
     3481 gaaattattg atgaaaatgg ccaaactcta ggagtgaata ttgatccaca aacatttaaa
     3541 ctctcagggg caatgccggc aactgcaatg aaaaaattaa cagaagctga aggagctaaa
     3601 tttaacacgg caaatttacc agctgctaag tataaaattt atgaaattca cagtttatca
     3661 acttatgtcg gtgaagatgg agcaacctta acaggttcta aagcagttcc aattgaaatt
     3721 gaattaccat tgaacgatgt tgtggatgcg catgtgtatc caaaaaatac agaagcaaag
     3781 ccaaaaattg ataaagattt caaaggtaaa gcaaatccag atacaccacg tgtagataaa
     3841 gatacacctg tgaaccacca agttggagat gttgtagagt acgaaattgt tacaaaaatt
     3901 ccagcacttg ctaattatgc aacagcaaac tggagcgata gaatgactga aggtttggca
     3961 ttcaacaaag gtacagtgaa agtaactgtt gatgatgttg cacttgaagc aggtgattat
     4021 gctctaacag aagtagcaac tggttttgat ttgaaattaa cagatgctgg tttagctaaa
     4081 gtgaatgacc aaaacgctga aaaaactgtg aaaatcactt attcggcaac attgaatgac
     4141 aaagcaattg tagaagtacc agaatctaat gatgtaacat ttaactatgg taataatcca
     4201 gatcacggga atactccaaa gccgaataag ccaaatgaaa acggcgattt gacattgacc
     4261 aagacatggg ttgatgctac aggtgcacca attccggctg gagctgaagc aacgttcgat
     4321 ttggttaatg ctcagactgg taaagttgta caaactgtaa ctttgacaac agacaaaaat
     4381 acagttactg ttaacggatt ggataaaaat acagaatata aattcgttga acgtagtata
     4441 aaagggtatt cagcagatta tcaagaaatc actacagctg gagaaattgc tgtcaagaac
     4501 tggaaagacg aaaatccaaa accacttgat ccaacagagc caaaagttgt tacatatggt
     4561 aaaaagtttg tcaaagttaa tgataaagat aatcgtttag ctggggcaga atttgtaatt
     4621 gcaaatgctg ataatgctgg tcaatattta gcacgtaaag cagataaagt gagtcaagaa
     4681 gagaagcagt tggttgttac aacaaaggat gctttagata gagcagttgc tgcttataac
     4741 gctcttactg cacaacaaca aactcagcaa gaaaaagaga aagttgacaa agctcaagct
     4801 gcttataatg ctgctgtgat tgctgccaac aatgcatttg aatgggtggc agataaggac
     4861 aatgaaaatg ttgtgaaatt agtttctgat gcacaaggtc gctttgaaat tacaggcctt
     4921 cttgcaggta catattactt agaagaaaca aaacagcctg ctggttatgc attactaact
     4981 agccgtcaga aatttgaagt cactgcaact tcttattcag cgactggaca aggcattgag
     5041 tatactgctg gttcaggtaa agatgacgct acaaaagtag tcaacaaaaa aatcacgatc
     5101 ccacaaacgg gtggtattgg tacaattatc tttgctgtag caggggctgt gattatgggt
     5161 attgcagtgt acgcatatgt taaaaacaac aaagatgagg atcaacttgc ttaagtaaga
     5221 gagaaaggag ccattgatga caatgcagaa aatgcagaaa atgattagtc gtatcttctt
     5281 tgttatggct ctgtgttttt ctcttgtatg gggtgcacat gcagtccaag cgcaagaaga
     5341 tcacacgttg gtcttgcaat tggagaacta tcaggaggtg gttagtcaat tgccatctcg
     5401 tgatggtcat cggttgcagg tatggaagtt ggatgattcg tattcctatg ataatcgggt
     5461 gcaaattgtg agagacttgc attcgtggga tgagaataaa ctttcttctt tcaaaaagac
     5521 ttcgtttgag atgaccttcc ttgagaatca gattgaagta tctcatattc caaatggtct
     5581 ttactatgtt cgctctatta tccagacgga tgcggtttct tatccagctg aatttctttt
     5641 tgaaatgaca gatcagacgg tagagccttt ggtcattgta gcgaaaaaag cagatacggt
     5701 gacaacaaag gtgaagctga taaaggtgga tcaagaccac aatcgcttgg agggtgtcgg
     5761 ctttaaattg gtatcagtag caagagatgg ttctgaaaaa gaggttccct tgattggaga
     5821 ataccgttac agttcttctg gtcaagtagg gagaactctc tatactgata aaaatggaga
     5881 gattgttgtg acaaatcttc ctcttgggac ctatcgtttc aaggaggtgg agccactggc
     5941 aggctatact gttacgacga tggatacgga tgtccagttg gtagatcatc agctggtgac
     6001 gattacggtt gtcaatcaga aattaccacg tggcaatgtt gactttatga aggtggatgg
     6061 taggaccaat acctctcttc aaggggcaat gttcaaagtc atgaaagaag aaaacggaca
     6121 ctatactcct gttcttcaaa atggtaagga agtagttgtg gcatcaggga aagatggtcg
     6181 tttccgagtg gaaggtctag agtatgggac atactattta tgggagctcc aagctccaac
     6241 tggttatgtt caattaacat cgcctgtttc ctttacaatc gggaaagata ctcgtaagga
     6301 actggtaaca gtggttaaaa ataacaagcg accacggatt gatgtgccag atacagggga
     6361 agaaaccttg tatatcttga tgcttgttgc cattttgttg tttggtagtg gctattatct
     6421 tacgaaaaaa acaaataact gatattcaat gtacatcatt atgaaaaaga tagcaggctg
     6481 aagggaagac cagagtactc tgaggtgatg ttaatcagga atcatggtga tttggcatga
     6541 atcataataa cggatatgag gctgggcaga ttgtgccagc ctcattgtgg gttattgttt
     6601 gtaaaacgat aggactggtc tggtaatcat tttaggaatg gacaggactg ggattctgat
     6661 ttaaaatgga tggtgaatca gaaagaaatg agattttctc gtttctctta gcagatagga
     6721 ttgtctgtta ggaaaagcga taaaatgatg agtttgaagt taaaggaatg ctgataaaaa
     6781 atggcaaaaa caaaaaagca aaaacgaaac aatctcctat taggagtggt atttttcatt
     6841 ggaatggcgg taatggcgta tccgctggtg tctcgcttgt attatcgagt ggaatcaaat
     6901 caacaaattg ctgactttga taaggaaaaa gcaacgttgg atgaggctga cattgatgaa
     6961 cgaatgaaat tggcacaagc cttcaatgac tctttgaata atgtagtgag tggcgatcct
     7021 tggtcggaag aaatgaagaa aaaagggcga gcagagtatg cacgcatgtt agaaatccat
     7081 gagcggatgg ggcatgtgga aatccctgct attgatgtag atttgccggt ttatgctggt
     7141 actgctgaag aggtattgca gcaaggcgct ggacatctag agggaacttc tctaccgatc
     7201 ggaggcaatt cgacccatgc ggtgattacg gcacatacag gtttgccaac ggctaagatg
     7261 tttacggatt tgaccaaact taaagttggg gataagtttt atgtgcacaa tatcaaggaa
     7321 gtgatggcct atcaagtgga tcaagtaaag gtgattgagc cgacgaactt tgatgattta
     7381 ttgattgtac caggtcatga ttatgtgacc ttgctgacct gtacgccata catgatcaat
     7441 acccatcgtc tattggttcg ggggcatcgg ataccgtacg tagcagaggt tgaggaagaa
     7501 tttattgcgg caaacaaact cagtcatctc tatcgctacc tgttttatgt ggcagttggt
     7561 ttgattgtga ttcttttatg gattattcga cgcttgcgca agaagaaacg gcaatcagaa
     7621 agagctttga aagcattgaa ggaagctact aaggaagtga aggtagagga tgagtaagag
     7681 tagatattca cggaaaaaga gcgtgaaaaa gaagaaaaat ccgttcattc ttcttctgat
     7741 ttttttggtg gggcttgccg ttgcgatgta tcccttggtg tctcgttatt attatcgtat
     7801 tgagtcaaac gaggttatta aagagtttga tgagacggtt tcccagatgg ataaggcgga
     7861 acttgaggag cgttggcgct tggctcaagc cttcaatgcg accttgaaac catctgaaat
     7921 cctcgatcct tttacagatc aggaaaagaa acagggagtt tcagaatatg ctaacatgct
     7981 aaaagttcat gagcgtatcg gatatgtaga aattcctgcg attgaacagg aaatccccat
     8041 gtatgttggc acaagtgaag acattcttca gaaaggggca gggctgttag aaggggcttc
     8101 gctgcctgtt ggaggtgaaa atacccatac agtgatcact gctcacagag gattgccaac
     8161 ggcagaattg ttcagtcaat tggataagat gaagaaaggg gatatctttt atcttcacgt
     8221 tttagaccag gttttggcct atcaagtgga tcagatagtg acggtggagc cgaatgattt
     8281 tgagcctgtt ctgattcaac atggacaaga ttatgcgact ttactgactt gtacgccata
     8341 catgattaac agccaccgtt tgttggtacg tgggaaacgg attccatata cagcgccgat
     8401 tgctgagcgg aatcgagcgg tgagagagcg tgggcaattc tggttgtggt tattactagg
     8461 agcgatggcg gtcatccttc tcttgctgta tcgcgtgtat cgtaatcgac ggattgtcaa
     8521 aggactagaa aagcaattgg aggggcgtca tgtcaaggac taaactacga gccttattgg
     8581 gatacttgtt gatgttggta gcctgtttga ttcctattta ttgttttgga cagatggtgt
     8641 tgcagtctct tggacaggtg aaaggtcatg ctacatttgt gaaatccatg acaactgaaa
     8701 tgtaccaaga acaacagaac cattctctcg cctacaatca acgcttggct tcgcaaaatc
     8761 gcattgtaga tccttttttg gcggagggat atgaggtcaa ttaccaagtg tctgacgacc
     8821 ctgatgcagt ctatggttac ttgtctattc caagtttgga aatcatggag ccggtttatt
     8881 tgggagcaga ttatcatcat ttagggatgg gcttggctca tgtggatggt acaccgctgc
     8941 ctctggatgg tacagggatt cgctcagtga ttgctgggca ccgtgcagag ccaagccatg
     9001 tctttttccg ccatttggat cagctaaaag ttggagatgc tctttattat gataatggcc
     9061 aggaaattgt agaatatcag atgatggaca cagagattat tttaccgtcg gaatgggaaa
     9121 aattagaatc ggttagctct aaaaatatca tgaccttgat aacctgcgat ccgattccta
     9181 cctttaataa acgcttatta gtgaattttg aacgagtcgc tgtttatcaa aaatcagatc
     9241 cacaaacagc tgcagttgcg agggttgctt ttacgaaaga aggacaatct gtatcgcgtg
     9301 ttgcaacctc tcaatggttg taccgtgggc tagtggtact ggcatttctg ggaatcctgt
     9361 ttgttttgtg gaagctagca cgtttactac gagggaaata aaaagaaatg aaaggaaagc
     9421 taaggctgtt cctttttccg gctctttgtc aactgtagtg ggttgaagaa aagctaagct
     9481 tgagaaagga caaatttcgt cctttctttt ttgatgttca aagc
//
LOCUS       DAVAAL010000067         9331 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVAAL010000067 DAVAAL010000000
VERSION     DAVAAL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.068"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(163..633)
                     /locus_tag="VQM60_001332"
     CDS             complement(163..633)
                     /locus_tag="VQM60_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET1081196.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(650..2923)
                     /locus_tag="VQM60_001333"
     CDS             complement(650..2923)
                     /locus_tag="VQM60_001333"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET1081197.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            3270..6371
                     /locus_tag="VQM60_001334"
     CDS             3270..6371
                     /locus_tag="VQM60_001334"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET1081198.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRVSFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            6454..7461
                     /gene="pfkA"
                     /locus_tag="VQM60_001335"
     CDS             6454..7461
                     /gene="pfkA"
                     /locus_tag="VQM60_001335"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET1081199.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAALVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            7520..9025
                     /gene="pyk"
                     /locus_tag="VQM60_001336"
     CDS             7520..9025
                     /gene="pyk"
                     /locus_tag="VQM60_001336"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET1081200.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATNSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAIEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
BASE COUNT         2857 a         1615 c         2074 g         2785 t
ORIGIN      
        1 gttgacgcgg attgaagaga ttttcgaaga gtataaatct tcctaggata aagcaaaacg
       61 catagtatca agggttttca acacttgata ctatgcgttt tctgatgtta aagactttct
      121 accaggtttt ttaaaagcat aattgttagt tgtagtcatt tattattctt caaagaaaaa
      181 tggtggggcg aattttttca gttcttcaaa gcacttttga gcagtatctg catcttcaca
      241 gatgataaga cagacatcat taccacaaag ggtagcgata gcgtcaggga agctcaaagt
      301 atcaatgata gaaccaaagg attgagccag tccaggaagg gtttttagta ggacttggtg
      361 ttgaactggg cgcatccaga caagggcgtc ttccatgtag agttcgagac gtttttccca
      421 ttttgagatg gaaccattgt taagaacata ataagcgcta tcttcttcgc ggacttttga
      481 taggttcata tttttgatgt cgcgtgagag ggttgcctgg gttacttgaa tgtcgttctt
      541 agcaagaagg gcttgcaact cagcctgtgt atgaatcttg ttttttgtga taagagcgcg
      601 tataagttgg tggcggtgtt ctgatttatt cataataatg taactccttt tagcaaggta
      661 aggtaagcat ggactgagcg aggtcgacag tcaagtggta gtctgtattg tcacggatgg
      721 tgatttcaaa gtcagtagta tagaggacta aacggagagt gtctccttct tttagcttgt
      781 aaatagttgg ctgcagttca aattgaacgt ccatccattc atctgcagta atatcctcta
      841 ctaacagtaa atcatttcta ttttgtaaat taaggtaacc ttttgtcacg actcgttgtg
      901 cctctggtct aaatggcaat tcacagagat tttccaacat gtgatagcga ccgttgtcaa
      961 tggttctagc acttaaaata gctggataag gttgtaggta tttcttttgc ccaaattcta
     1021 gcagttgggc agataagagc cccttgtttg tactggattt gatacgaaga ttgagctgag
     1081 cgcgaccgtt taggtggaga tctttagtca caggaaggtt aatagtaatc tgattagctt
     1141 tcccttgata gagctctgta ttgaaggttt ggtatgtctt accatagcgc tcaaaatcct
     1201 tatctgggta ctggttttga atagcttgct cttcttgacc aagtgagaag gtttcacagt
     1261 tttcttgccc accgaagtta tcaagtgata accaagtctg tggagctgta ttgtcctgcc
     1321 agataacagt aggaagttga aagtctgttt cctgtcctag taatttcttg gtcaataagg
     1381 catttatgga ctcacggaag tcaattgatt gccaattgtt catgtaaaca tgggcaccat
     1441 tatggaaaaa gagatgcttg tgtatatgag taggaagagc atggaacatc tggtaaacat
     1501 gaagtggttt gacattccaa tcctgagaac catgagtaaa gacaacctct gcctttactt
     1561 tatgggcatt gagcagataa ttgcggtcat gccaaaactg attgtagtcc ccagtttttc
     1621 ggtctagctg agctttcact ttttctaagt cagcttggtg agcttcattg ccacggatat
     1681 agtcgccagc taagagatta cgagaatagg ttaactcagc aagggagtca aagtcctcac
     1741 ctggataacc acctgggcta gtcaccagac cgttttcacg gtagtagttg taccatgatg
     1801 aaattcctgc ctcggcaatg ataacttcta aaccatcgac tcctgtagtc gcaagaccat
     1861 tggacatggt acctagatag gaaagtcctg ttgtagcaac ttttccgttt gaccaatcag
     1921 ccttgacttg acgctggcgc gtgtgatcag taaaggcacg gcaacgaccg ttaagccaat
     1981 cgatgacgtt tttataagcc tcgatttgct ggtagtctcc attagtcatg aaacctgtcg
     2041 agtctttggt accaacacct gagacataga gattggcaaa gcctctcgga aggaagtagt
     2101 cgtttagtgt atagctagag ttgatgtgag ttagcttttc ctcagcctct gctataagct
     2161 cagctttacc ttggggttgg acgagattta gttgaggttt ctctagctca atcttgtgag
     2221 gaagcttaac ctcaagctcg ccctccatct tgtagagagc cttgtcacta gccttgtcat
     2281 tggttccctg atgataaggg ctggctgtca tgatggcagg gatttttcca tcaaaacgag
     2341 ggcgaataat gctaaccttt actaggtctg atagcccttt ttggtcagta tcgacacgag
     2401 actcaacgta aacgacttca cgaatgacat cctggttaga aaaagtagcc aaactcttgc
     2461 cgttaaagta gtggtagtca ttatcctccg gaataagacc atcactaaca agttggtcga
     2521 taagagtatt tcctttttta gtgcgagtat tgagtaactg atagagattt tcaatcaagt
     2581 caccatatat aatgggaaat ccagtttctt tacgaaaaac gtcactatct tcgaagtcaa
     2641 ccaaataaga aaagcctaaa agttgaaaag caacagtata aaaaatatct gctgtcagtt
     2701 catcttctga ttgaaaaaat gtcagcaggt ctgttttttt atcagctgct aggatagaaa
     2761 gtgggtagtt ggtgtcttga taagtgaaaa agaaacgacg taaaaaggtt tcaagtgagt
     2821 ctttgtgatt ggctgtattt tgtaaatcaa agccacattt ttttagttca gataagacat
     2881 tttcttttgg aaaattgata taactatatt gattaaaacg catagaacct ccatatagaa
     2941 tgacagttaa ggttattata tcaaaaaaaa agcagaaagg gaattgttaa cttcaaaagg
     3001 aaataatcca ataaaaatga ataaagtact aaattcaata tagagaacag agtaacaata
     3061 agaataaata gatagggtat aaaagttcta ggagatttat attatatgct ttctattttt
     3121 atatacaata tagtataaat ataaaaatga tgacaaaaat acaaatgaat agaaaataaa
     3181 ttagtaagct gatgaaattt ttctcaagag aagccattta taggtgaaaa tggtataata
     3241 tagtgagaag gatagaggag aagtgtaaat tgatcgcaca actagataca aaaacagtct
     3301 atagttttat ggaaagcgtc atttcgatcg aaaagtatgt gagagcagct aaagaatacg
     3361 gctacactca tttggctatg atggatattg acaatcttta tggcgctttc gactttctag
     3421 agattacaaa aaaatacggc attcatcctt tgctagggct tgaaatgaca gtgtttgtag
     3481 atgatcagga agtgaatttg cgctttttag ctctatctag tgtgggctat cagcagttga
     3541 tgaagctttc gacagccaag atgcaggggg agaaaacttg gtcagtcctg tcccagtacc
     3601 tggaggatat cgcggtcatt gtgccttatt ttgatagagt tgagtcgtta gaactaggct
     3661 gtgattacta tataggggtt tatccagaaa cactagcaag cgaatttcat catcctatct
     3721 tacctcttta tcgggtcaac gcttttgaaa gcagggatag agaagttctt caagttttaa
     3781 cagcgattaa agaaaatcta ccgctcagag aagttccctt gcgttcgaga caagatgtct
     3841 ttatatcagc aagttcttta gagaaactat tccaagagcg ttttccgcaa gctttggaca
     3901 atttagaaaa gcttatttca ggcatttctt acgacttgga tactagtctg aaactgcctc
     3961 gttttaatcc agctagacca gcagtagagg agttgagaga gcgtgctgaa ctggggcttg
     4021 ttcagaaggg gttgactagt aaagaatatc aagatagact agaccaagaa ttgtctgtta
     4081 ttcatgatat gggctttgat gattatttct tggttgtttg ggatttgttg cgttttggac
     4141 gatcgaatgg ctattatatg ggaatgggaa ggggttctgc agtaggcagt ttggtttctt
     4201 atgccttaga catcacgggg attgacccag tagagaaaaa tctgattttt gaacgctttc
     4261 ttaatcgtga acgctatacc atgcctgata ttgatattga tatcccagat atttatcgtc
     4321 cagattttat cagatatgtt ggtaataaat atggtagtaa acatgcggca caaatcgtta
     4381 ctttttcaac ctttggagcc aagcaagctc ttcgagatgt cttgaaacgc tttggtgtgc
     4441 cagagtatga attatctgca attactaaga aaatcagttt tcgtgacaat cttaagtcgg
     4501 cctatgaggg caatctccag tttcgtcagc aaatcaatag taagttagaa taccaaaaag
     4561 cttttgagat tgcttgcaag atagagggct atccaagaca aacctctgtc catgcggctg
     4621 gtgttgtaat tagtgaccaa gatttaacca actatattcc tctaaagtat ggtgatgaaa
     4681 ttccactgac tcagtatgat gctcatggag ttgaggctag cggacttttg aagatggact
     4741 ttctgggact acgaaatttg acctttgtcc agaagatgca agagttgctt gctgaaatag
     4801 aaggtattca ccttaaaatt gaagaaatag atttggaaga caaagaaacg ttagatttat
     4861 ttgcctctgg aaatacaaaa ggtatctttc aatttgagca accaggtgcc attcgtctgc
     4921 ttaagcgtgt gcaaccagtc tgttttgaag atgtcgtcgc gactacttct ctaaatcgac
     4981 cgggtgctag tgactatatc aataattttg tggcaagaaa gcatgggcag gaagaagtga
     5041 ctgttctgga tccagtactg gaggatattt tggctccaac ctacggcata atgctctatc
     5101 aggagcaggt tatgcaggtt gcccagcgat ttgccggatt tagtcttggg aaagccgata
     5161 ttttgcgtcg ggctatgggg aaaaaggatg cctctgccat gcatgagatg agggtttcct
     5221 ttattcaagg ttcattagaa gctggtcata ctgtggaaaa agcagagcag gtctttgatg
     5281 ttatggagaa gtttgcaggt tatggtttta acaggtcaca cgcctatgcc tactcagcct
     5341 tggccttcca gttggcttat tttaaaacac attatccagc cattttttat caggtcatgt
     5401 taaattcttc caacagtgat tacttaatag atgcacttga agcaggtttt gaagtagcct
     5461 ctctatccat caacaccatt ccctatcacg ataaaattgc caacaaggcc atctatctag
     5521 gtttgaaatc cattaaagga gtcagtaatg atttagctct ctggattatt gaaaatagac
     5581 cttattctaa cattgaagat tttatagcta aattacctga gaattatctg aaacttcctc
     5641 tgctagaacc tttggtaaaa gttggtcttt tcgattcatt tgaaaaaaat cgtcaaaaag
     5701 tatttaataa tttagctaat ctatttgaat ttgtgaaaga gttgggaagt ttgtttggag
     5761 atgctattta tagttggcag gaatcggaag attggacgga acaagaaaaa ttttatatgg
     5821 aacaagagct tttagggata ggtgtcagca aacatccact acaagctatt gcaagtaagg
     5881 ctatttaccc gattacccca atcggaaatt tgtcagaaaa tagctatgct attatcttgg
     5941 ttgaagttca gaaaataaaa gtgattcgta ccaaaaaggg tgaaaatatg gccttcttac
     6001 aggcagatga tagtaagaaa aaattggatg tcactctctt ttcagactta tatcgtcagg
     6061 ttggacagga aataaaagag ggagccttct actatgtaaa aggaaaaata caatcacgtg
     6121 atggccgtct gcaaatgatt gcacaagaaa taagagaagc agttgctgaa cgcttttgga
     6181 tacaggtgaa aaatcatgaa tcggatcaag aaatttcacg cattttagaa caatttaaag
     6241 gcccaatccc agtcatcatc cggtatgaag aggaacagaa aaccatcgtt tctccccatc
     6301 attttgtagc taaatccaat gaattagagg agaaattgaa tgaaatcgtt atgaaaacga
     6361 tttatcgcta aaaatacgga aaatagaaga attttaaaat caaatgtgat ataatcaaga
     6421 agaatgttaa aagaaaaagg agcataaacc aatatgaaac gtattgctgt tttgactagt
     6481 ggcggagacg cacctggtat gaacgctgcc atccgtgcag ttgttcgtca agcaatttca
     6541 gaaggaatgg aagtgtttgg tatctatgac ggatatgctg gtatggttgc cggtgaaatt
     6601 catcccctag atgcagcttt agtaggggac atcatttctc gtggtggtac ttttcttcac
     6661 tcagctcgtt atccagagtt cgctcaactt gaagggcaac ttaaagggat tgagcaattg
     6721 aaaaaacacg gaattgaagg tgtagttgtt atcggtggtg acggatctta ccacggcgct
     6781 atgcgtttga ctgaacatgg cttcccagct attggtcttc caggtacaat cgataacgat
     6841 atcgttggta ctgactttac aatcggtttt gacacagcgg ttactactgc catggacgct
     6901 atcgataaga ttcgtgatac atcatcaagt caccgtcgta cttttgtaat cgaagttatg
     6961 ggacgtaacg ctggtgatat cgctctttgg gctggtattg caactggtgc tgatgaaatc
     7021 atcatccctg aagcaggctt caagatggaa gatatcgtag caagcatcaa agctggttat
     7081 gaatgtggta aaaaacacaa tatcatcgtc ttagctgaag gtgtgatgtc agcggctgaa
     7141 tttggtcaaa aacttaaaga agctggagat acaagcgacc ttcgtgtaac agaacttgga
     7201 catattcaac gtggtggttc tccaactgcg cgtgaccgtg ttttggcgtc acgtatgggt
     7261 gcacatgctg ttaaacttct taaagaaggt atcggtggtg ttgcggttgg tattcgtaac
     7321 gaaaaaatgg ttgaaaatcc aattcttggt actgcagaag aaggggcatt gtttagcctt
     7381 actgcagaag gtaagattgt ggttaacaac ccgcacaaag ccgatattga gctatctagc
     7441 ttgaataaga gcttgtcata atttacaatt tagttaataa gaccaaaaag gtcatatata
     7501 taaaggagtc acaaaaatca tgaacaaacg tgtaaagatc gttgcaactt tgggtcctgc
     7561 ggtagaaatc cgtggtggta aaaaattcgg tgaggatgga tactggggtg aaaaacttga
     7621 tgttgaagct tcagctaaaa acattgctaa attgattgaa gctggtgcta acacattccg
     7681 attcaacttc tcacacggcg accaccaaga acaaggtgag cgtatggcaa ctgttaaact
     7741 tgcggaaaaa attgcaggta aaaaagttgg tttccttctt gatacaaaag gacctgaaat
     7801 ccgtacagaa ttgttcgaag gtgaagctaa agaatattca tacaaaactg gtgaaaaaat
     7861 tcgtgttgca actaaacaag gaatcaaatc aactcgtgaa gtgattgcgt tgaacgttgc
     7921 tggtgctctt gatatctatg atgatgttga agttggtcgt caagttttgg ttgacgatgg
     7981 taaacttggt cttcgtgtgg ttgctaaaga tgatgcaact cgtgaatttg aagttgaagt
     8041 tgaaaacgat ggtatcatcg ctaaacaaaa aggtgtgaac atccctaaca ctaaaattcc
     8101 tttcccagct cttgctgaac gcgataacga cgatatccgt ttcggtcttg aacaaggtat
     8161 caacttcatc gcaatttcat tcgtacgtac tgcaaaagat gtgaacgaag ttcgtgcaat
     8221 ctgtgaagaa actggaaacg gacatgttca attgttcgct aaaatcgaaa accaacaagg
     8281 tatcgataac ttagacgaaa tcatcgaagc agctgatggt attatgattg ctcgtggtga
     8341 tatgggtatc gaagtaccgt tcgaaatggt tccagtttat caaaaaatga ttatcaagaa
     8401 agtcaatgct gcaggtaaag ttgttatcac tgcaacaaac atgcttgaaa caatgactga
     8461 aaaaccacgt gcaactcgtt cagaagtatc agacgtattc aacgctgtta tcgacggaac
     8521 tgacgctaca atgctgtcag gcgagtctgc aaacggtaaa tacccactcg agtcagtaac
     8581 tacaatggct acaatcgaca agaacgctca agctcttctt aatgaatacg gacgtcttga
     8641 ttcagattca tttgagcgta actctaagac agaagtaatg gcttctgctg ttaaagatgc
     8701 tactaactca atggatatca aattggttgt aactcttact aagacaggtc atactgcacg
     8761 tttgatttct aaataccgtc caaatgctga catcttagca ttgacatttg acgaattgac
     8821 agaacgtggc ttgatgttga actggggtgt tatcccaatg ttgacagatg ctccatcttc
     8881 aactgacgat atgttcgaaa tcgctgaacg taaagcgata gaagcaggtc tcgttgagtc
     8941 aggcgatgat atcgttatcg ttgctggtgt gccagtagga gaagctgttc gcacaaacac
     9001 aatgcgtatc cgcacagtac gttaagaaaa atataaaaac ctatcatatc cagctttaga
     9061 gcttgtgtga taggtttttt gtatagaggg taacaaagag gcaaaacttt cataatcgat
     9121 tgaaataaga tatgcacaaa ttgattagaa agtcaaatga atttctacaa atgttttagc
     9181 aatcgtaatg tacttgtcta gattcgatct gatatatttt cgatttaatg atatggtatt
     9241 taaaacctcc aaagtagctt gctccattct tttacttacg tgagtgtaga tgttatttac
     9301 tgttttagcg tttttgtgtt ccactctaac c
//
LOCUS       DAVAAL010000068         9207 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVAAL010000068 DAVAAL010000000
VERSION     DAVAAL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.069"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..109
                     /locus_tag="VQM60_001337"
     CDS             <1..109
                     /locus_tag="VQM60_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET1081201.1"
                     /translation="NVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE"
     gene            178..1044
                     /locus_tag="VQM60_001338"
     CDS             178..1044
                     /locus_tag="VQM60_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1081202.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGHMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            1041..1799
                     /locus_tag="VQM60_001339"
     CDS             1041..1799
                     /locus_tag="VQM60_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081203.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRVIQDLKQEEKAKMKISDYYQLFE"
     gene            1889..3649
                     /gene="dnaG"
                     /locus_tag="VQM60_001340"
     CDS             1889..3649
                     /gene="dnaG"
                     /locus_tag="VQM60_001340"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET1081204.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYQAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            3652..4761
                     /gene="rpoD"
                     /locus_tag="VQM60_001341"
     CDS             3652..4761
                     /gene="rpoD"
                     /locus_tag="VQM60_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET1081205.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            4775..5104
                     /locus_tag="VQM60_001342"
     CDS             4775..5104
                     /locus_tag="VQM60_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET1081206.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            5219..6262
                     /gene="cpoA"
                     /locus_tag="VQM60_001343"
     CDS             5219..6262
                     /gene="cpoA"
                     /locus_tag="VQM60_001343"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET1081207.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            6278..7603
                     /locus_tag="VQM60_001344"
     CDS             6278..7603
                     /locus_tag="VQM60_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET1081208.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            7634..7810
                     /locus_tag="VQM60_001345"
     CDS             7634..7810
                     /locus_tag="VQM60_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET1081209.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            7869..8003
                     /locus_tag="VQM60_001346"
     CDS             7869..8003
                     /locus_tag="VQM60_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET1081210.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            8067..>9207
                     /gene="obgE"
                     /locus_tag="VQM60_001347"
     CDS             8067..>9207
                     /gene="obgE"
                     /locus_tag="VQM60_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET1081211.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSG"
BASE COUNT         2758 a         1634 c         2152 g         2663 t
ORIGIN      
        1 caatgtcttt tcaactggta agtcagtcat caataaaaaa atagcacaag aactaggtat
       61 tactattcct gagtctgttc tcaaagaagc aggacaagtc atcgaataaa taaggtaggg
      121 aggaggagac tcttcccatt ttttaaaaag atgaatatag aaaggattaa ataaaatatg
      181 attgtttcca ttatttctca aggatttgtc tgggctattc taggtctggg aatctttatg
      241 acatttagga ttttaaactt tccagatatg acgacagaag gttccttccc tcttggggga
      301 gctgttgctg tcactttgat aaccaaaggc gtgaacccat ttttagcgac acttgttgct
      361 gtaggagcag gttgtttggc tggaatggca gcaggccttc tttatacaaa agggaagatc
      421 ccaaccttgc tctcagggat tttggtgatg acttcttgtc actcaatcat gctcttgatt
      481 atgggacgtg cgaatttagg cctgcttgga accaagcaaa ttcaggatgt tttgcctttt
      541 gattcggatt tgaatcaact cttgacaggt ctcatctttg tgagtattgt tattgctctc
      601 atgctctttt tcttggacac taaactcgga caagcctata ttgctacagg ggataatcct
      661 gatatggcta gaagtttcgg gattcatact ggacacatgg agctcatggg cttggtctta
      721 tcaaatggtg tgattgccct tgcaggtgcc ctcattgctc agcaagaagg ttatgccgat
      781 gtgtctcgag ggatcggggt tatcgttgtg gggcttgcaa gtttgattat tggagaagtt
      841 attttcaaga gtttgagctt ggcagagcgt ttggttacta tcgttgtagg ttctatcgct
      901 tatcaatttt tagtgtgggc agttatcgca cttggcttta atacaagtta ccttcgttta
      961 tacagtgcct tgattttagc agtctgcctc atgattccaa catttaagca aacaatcttg
     1021 aaaggagcca agttaagcaa atgacagcaa ttgtagaatt aaaaaatgca accaaaatcg
     1081 ttaaaaatgg ctttgatgaa gaaaagatta ttttaaatga tgtttcctta gaaatttttg
     1141 aacaggactt tatcacgatt ttgggtggaa atggtgctgg taaatcaacc ctctttaaca
     1201 ctattgcagg aaccatatca ctaaccagtg gaactatccg tattttaggt gaggatgtta
     1261 ccaagttttc accagagaag cgcgctaagt atctgtctcg agtcttccaa gatccaaaga
     1321 tggggacagc tcctcgtatg acagtggcag agaatctcct gattgccaag tttcgtggtg
     1381 aaaagcgtgg actatttccg agacgtctgg ctagctataa ggataagttt caggcgacca
     1441 ttgaaaaagt gggaaacggt cttgagaaac acttgaatac accgattgag ttcttatctg
     1501 gtggacaaag acaggctttg agtctcttga tggcaacctt gaagcgacct gaattactct
     1561 tgttagacga gcatactgct gccctggatc caaagactag tgttgctttg atggaattga
     1621 cagatgaatt tgttaagaaa gatcaattga cagcccttat gattacccat catatggaag
     1681 atgctctcaa atatggcaat cgcttgattg tcatgaaaga aggacgggtt atccaagatt
     1741 tgaaacaaga agaaaaagca aaaatgaaaa tctctgatta ttatcaactc tttgaataaa
     1801 gtagggaaaa tgagtgaaat ggtttacttt ttttctgaaa taaagtatac tatataaagt
     1861 aaactatgat aacatggagg tattgtgtat ggttgacaaa caagtcattg aagaaatcaa
     1921 aaacaatgcc aacattgtgg aagtcatagg agatgtgatt tctttacaaa aggcaggacg
     1981 gaactatcta gggctctgtc cttttcatgg tgaaaaaaca ccttctttca acgttgtaga
     2041 ggacaagcag ttttaccact gttttggttg tggtcgctca ggtgatgtct ttaaattcat
     2101 cgaggagtac caaggggtta cctttatgga ggctgtccaa atcttaggtc agcgtgtcgg
     2161 gattgaggtt gaaaaaccgc tttatagtga acagaagcca gcctcgcctc accaagctct
     2221 ttatgatatg cacgaagatg cggctaaatt ttaccatgct attctcatga caacgactat
     2281 gggcgaagag gccagaaatt acctttatca gcggggtttg acagatgaag tgcttaaaca
     2341 tttttggatt ggtttagcac ctccagaacg aaactatctc tatcaacgtt tgtctgatca
     2401 gtatcgtgaa gaagatttac tggattcagg cctgttttat ctctcggatg ccaatcaatt
     2461 tgtagacacc tttcacaatc gcattatgtt tcccctgaca aatgaccaag gaaaggtcat
     2521 tgccttctca ggtcgtatct ggcaaaaaac agattcacaa acttctaagt ataaaaacag
     2581 ccgatcgact gcaattttta acaaaagtta cgaattatat catatggata gggcaaaaaa
     2641 atcttctgga aaaactagtg agatttacct gatggaagga ttcatggatg ttattgcagc
     2701 ctatcaggct ggaatcgaaa atgctgtggc gtcgatggga acggccttga gtcgagagca
     2761 tgttgagcat ctgaaaaggt taaccaagaa attggttctt gtttacgatg gagataaggc
     2821 tgggcaagcc gcgatattga aagcattgga tgaaattggt gatatgcctg tgcaaatcgt
     2881 tagcatgcct gataacttgg atcctgatga gtatctacaa aaaaatggtc cagaagactt
     2941 ggcctatcta ttaacgaaaa ctcgtattag tccgattgag ttctacattc atcagtacaa
     3001 acctgaaaac agtgaaaatc tgcaggctca gattgagttt attgaaaaaa tagctccctt
     3061 gattgttcaa gaaaagtcca tcgctgctca aaacagctat attcatattt tagctgacag
     3121 tctggcgtcc tttgattatg cccagattga gcagattgtt aatgagagtc gtcaggtgca
     3181 aaggcagaat cgcatggaag gaatttccag accgacgcca atcaccatgc ctatcaccaa
     3241 gcagttatcg gctattatga gggcagaagc ccatctactc tatcggatga tggaatcccc
     3301 tcttgttttg aacgattacc gtttgcgaga agactttgca tttgctacac ctgaatttca
     3361 ggtcttatat gacttgcttg gccagtatgg aaatcttcct ccagaagttt tagcagagca
     3421 gacagaggaa gttgaaagag cttggtacca agttttagct caggatttgc ctgctgagat
     3481 atcgccgcag gaacttagtg aagtagagat gactcgaaac aaggctctct tgaatcagga
     3541 caatatgaga atcaaaaaga aggtgcagga agctagccat gtaggagata cagatacagc
     3601 cctagaagaa ttggaacgtt taatttccca aaagagaaga atggagtaat aatggcaaca
     3661 aaacaaaaag aagtaacaac atttgacgta caggtagcag aatttatccg taatcataag
     3721 caaaaaggga cagcaacaga tgatgaaatc aatgcaagtc tggttattcc ttttaccttg
     3781 gacgctgatg ggattgaaga tctcttgcaa cggattcagg atgcaggcat ttctatcaca
     3841 gataacgaag gaaatccaag tgcgcgtgtt cttagcaatg aagaagaacc agaactcagc
     3901 gatgaggact tgattgggtc aacttctgct aaggtcaatg accctgtccg tatgtacttg
     3961 aaagaaatag gggtcgttcc tctcttgacc aatgaagagg agaaagagtt ggcactggct
     4021 gttgaagctg gcgatatcga agctaaacaa cgtcttgcgg aagccaatct tcgtttggtt
     4081 gtttccattg ccaaacgcta tgtcggtcgt ggcatgcagt tccttgactt gattcaagaa
     4141 ggaaatatgg gcttgatgaa ggcggttgac aagtttgact attctaaagg gttcaagttt
     4201 tcaacttatg caacttggtg gattcgtcag gctatcactc gtgctattgc ggatcaagct
     4261 cgtaccatcc gtatcccagt tcacatggtt gaaactatca ataaattggt tcgtgaacag
     4321 cggaatctcc ttcaagaatt ggggcaagat ccgacaccag aacagattgc tgaacgaatg
     4381 gatatgacac ctgataaggt tcgtgaaatc ttgaagattg cccaagaacc agtatctctt
     4441 gaaactccta tcggtgaaga ggacgatagc caccttggag actttatcga agatgaagtg
     4501 attgaaaatc cagtggatta tacgactcgt atcgtcttgc gtgagcaatt ggatgaaatc
     4561 ttagatactc ttacagaccg tgaagaaaat gttctgcgtc tacgttttgg actagatgat
     4621 ggaaaaatgc gcacacttga agatgtgggg aaagtcttta acgtaactcg tgagcgtatc
     4681 cgtcagattg aagcaaaggc tttgagaaaa ctacgccaac caagtcgcag caaaccgctt
     4741 cgtgatttta ttgaagacta agagtgagga aaatatggct tatacagaag agcaaattga
     4801 aaacatcaaa acacggattt taacagcctt ggaagaagtc atcgaccctg agttgggaat
     4861 cgatattgtc aatcttggtt tgatctatga gattcgtttt gacggtgaca cagggcaaac
     4921 agagattgac atgactttga caactatggg ttgtccctta gcagaccttt tgacagacca
     4981 gatttatgat gctatgatag aggttccaga agtaacggat actgaggtta aattagtctg
     5041 gtatccagcc tggacagttg aaaaaatgag tcgctatgcc cgcattgccc taggcattaa
     5101 gtaaacatat aaaagcatgt gagagactgt tggaaaagcg aggaaatttc ccctcttttc
     5161 ctctagtctc tcctttcttt tgctgatttt attcaaagaa aatgatataa tagtagttat
     5221 ggagaaaaag aaattacgca tcaatatgtt gagttcaagt gagaaagtag caggacaggg
     5281 agtttcaggt gcttaccgtg aattagttcg tcttcttcac cgtgctgcca aggaccaatt
     5341 gattgttaca gaaaatcttc cgatcgaggc agatgtgact cactttcata cgattgattt
     5401 tccctattat ttatcaacct tccaaaagaa acgctcaggg agaaagattg gctatgtgca
     5461 tttcttgcca gctacacttg agggaagttt gaaaattcca tttttcttaa agggaattgt
     5521 gaaacgctat gtattttctt tttacaaccg gatggagcac ttggttgtgg tcaatcctat
     5581 gtttattgag gatttggtag cagctggtat tccacgtgaa aaagtgacct atattcctaa
     5641 ctttgtcaac aaggaaaaat ggcatcctct accacaagaa gaggtagtca gactgcgcac
     5701 agatcttggt cttagtgaca atcagtttat cgtagtaggt gctgggcaag ttcagaaacg
     5761 taaagggatt gatgacttta tccgtctggc tgaggaattg cctcagatta cctttatctg
     5821 ggctggtggc ttctcttttg gtggtatgac agatggttat gaacactata agaaaattat
     5881 ggaaaatccc cctaaaaatt tgatttttcc aggcattgta tcgccagagc ggatgcgcga
     5941 attgtatgct ctagcggatc ttttcttgtt gcctagttac aatgagctct ttcctatgac
     6001 tattttagaa gctgcgagtt gtgaggctcc tattatgttg cgtgatttag atctctataa
     6061 ggtgattttg gagggaaatt atcgggcgac agcgggtaga gaagagatga aagaggctat
     6121 tttggaatat caagcaaatc ctgctgcctt aaaagatctc aaagaaaagg ctaagaatat
     6181 ttccagagag tattctgaag agcatctgtt acaaatctgg ttggactttt atgagaaaca
     6241 agccgcttta gggagaaagt aaaaagtgag gtaatctatg cgaattggtt tatttacaga
     6301 tacctatttt cctcaggttt ctggtgttgc gaccagtatt cgaaccttga aaacagaact
     6361 tgaaaagcag ggacatgctg tttttatctt tacgacgaca gataaggatg tcaatcgcta
     6421 cgaagattgg caaattatcc gcattccaag tgttcctttc tttgctttta aggatcgtcg
     6481 ctttgcctac cgaggtttta gcaaggcact tgaaattgct aaacagtatc agctagatat
     6541 tatccatact cagacagaat tttctcttgg cctgttgggg atttggattg cgcgtgaatt
     6601 gaaaattcca gtcatccata cctatcacac ccagtatgaa gactatgtcc attatattgc
     6661 taaggggatg ttgatccgac cgagtatggt caagtatctg gttagaggtt tcctgcatga
     6721 tgtggatggg gttatttgcc ctagtgagat tgtccgtgac ttgctatctg attataaggt
     6781 caaggttgaa aaacgggtca ttcctactgg gattgaatta gccaagtttg agcgtccgga
     6841 aatcaagcag gaaaatttga aagaactgcg tagtaaacta gggattcaag atggtgaaaa
     6901 gacgttgctt agtctttcga gaatctccta tgaaaaaaat attcaagcag ttttagtagc
     6961 ctttgctgat gttctgaaag aggaagacaa ggttaaactg gtagtagctg gggatggccc
     7021 ttatctgaat gacctcaaag agcaagccca gaacctagag attcaagact cagtcatctt
     7081 tacagggatg attgctccta gtgagacggc tctttactat aaagcggcgg attttttcat
     7141 ttcggcatcg acaagcgaaa cgcaaggttt gacctacttg gaaagcttag ccagtggaac
     7201 acctgtcatt gctcacggaa atccttattt gaacaacctc atcagtgata aaatgtttgg
     7261 aaccttgtac tatggagaac atgatttggc tggtgctatt ttggaagccc tgattgcaac
     7321 accagacatg aacgagcata ccttatcaga gaaattgtat gagatttcag ctgagaactt
     7381 tgggaaacga gtgcatgagt tttatctgga tgccattatt tcaaataact tccagaaaga
     7441 tttggctaaa gatgatacgg tcagtcagcg tatctttaag acagttttgt atcttcagca
     7501 acaggtggtt gctgtacctg taaaaggatc tagacgcatg ttgaaggctt caaaaacaca
     7561 gttgatcagt atgagagact attggaaaga ccatgaagaa tagaaagagg aacagctatg
     7621 aaaaaaacaa ttaatgagaa gcggtcgtga taaaaagatt gcgggtgttt gtgctggggt
     7681 ggcccattat ctggatatgg atccgactat cgttcaagtc atttggggtg ttcttacttg
     7741 ctgttacgga gctggaattg tagcttacat tattttatgg attatcgcac cagtagcaac
     7801 tgactattga aaactagctc aattcatgct aagataatag aaaatagaat gtaacgaagg
     7861 agagaaaaat ggcatttgga gataatggaa atcgtaaaaa aactatgttt gagaaaataa
     7921 ccttgtttat cgtgattatc atgctagtag caagtttatt gggaattttt gcaactgcaa
     7981 ttggtgccct cagtaatcta taaaatagat tcaagaaaat ttagtgactg ggatttccca
     8041 gccctttttt aaagtgagaa gaaataatga gtatgttttt agatacagct aagattaagg
     8101 tcaaggctgg taatggtggc gatggtatgg ttgcctttcg tcgtgaaaaa tatgtcccta
     8161 atggaggccc ttggggtggt gatggtggtc gtggaggcaa tgtggtcttc gttgtagacg
     8221 aaggactacg taccttgatg gatttccgct acaatcgtca tttcaaggct gattctggtg
     8281 aaaaagggat gaccaaaggg atgcatggtc gtggtgctga ggaccttaga gttcgagtac
     8341 cacaaggtac gactgttcgt gatgcggaga ctggcaaggt tttaacagat ttgattgaac
     8401 atgggcaaga atttatcgtt gcccacggtg gtcgtggtgg acgtggaaat attcgtttcg
     8461 cgacaccaaa aaatcctgca ccggaaatct ctgaaaatgg agaaccaggt caggaacgtg
     8521 agttacaatt ggaactaaaa atcttggcag atgtcggttt agtaggattc ccatctgtag
     8581 ggaagtcaac acttttaagt gttattacct cagctaagcc taaaattggt gcctaccact
     8641 ttaccactat tgtaccaaat ttaggtatgg ttcgcaccca atcaggtgaa tcctttgcag
     8701 tagccgactt gccaggtttg attgaagggg ctagtcaagg tgttggtttg ggaactcagt
     8761 ttctccgtca catcgagcgt acacgtgtta tccttcacat cattgatatg tcagctagcg
     8821 aaggccgtga tccatatgag gattacctag ctatcaataa agagctggag tcttacaatc
     8881 ttcgcctcat ggagcgtcca cagattattg tagctaataa gatggatatg cctgagagtc
     8941 aggaaaatct tgaagaattt aagaaaaaat tgtctgaaaa ttatgatgaa tttgaagagt
     9001 taccagctat cttcccaatt tctggattga ccaagcaagg tctggcaaca cttttagatg
     9061 ctacagctga attgttagac aagacaccag aatttttgct ctacgacgag tccgatatgg
     9121 aagaagaagc ttactatgga tttgacgaag aagaaaaagc ctttgaaatt agtcgtgatg
     9181 acgatgcgac atgggtactt tctggtg
//
LOCUS       DAVAAL010000069         9053 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVAAL010000069 DAVAAL010000000
VERSION     DAVAAL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.070"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(330..1178)
                     /gene="dprA"
                     /locus_tag="VQM60_001348"
     CDS             complement(330..1178)
                     /gene="dprA"
                     /locus_tag="VQM60_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET1081212.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLALSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1274..1963)
                     /locus_tag="VQM60_001349"
     CDS             complement(1274..1963)
                     /locus_tag="VQM60_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET1081213.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1975..2853)
                     /locus_tag="VQM60_001350"
     CDS             complement(1975..2853)
                     /locus_tag="VQM60_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET1081214.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2843..3712)
                     /gene="licA"
                     /locus_tag="VQM60_001351"
     CDS             complement(2843..3712)
                     /gene="licA"
                     /locus_tag="VQM60_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET1081215.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            complement(3729..4751)
                     /locus_tag="VQM60_001352"
     CDS             complement(3729..4751)
                     /locus_tag="VQM60_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET1081216.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4756..5463)
                     /locus_tag="VQM60_001353"
     CDS             complement(4756..5463)
                     /locus_tag="VQM60_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET1081217.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5799..7286
                     /gene="tacF"
                     /locus_tag="VQM60_001354"
     CDS             5799..7286
                     /gene="tacF"
                     /locus_tag="VQM60_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET1081218.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7296..8099
                     /locus_tag="VQM60_001355"
     CDS             7296..8099
                     /locus_tag="VQM60_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET1081219.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8101..8910
                     /locus_tag="VQM60_001356"
     CDS             8101..8910
                     /locus_tag="VQM60_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET1081220.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHHRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2851 a         1819 c         1523 g         2860 t
ORIGIN      
        1 tcactatact ttcccttata ctcaatgaaa atcaaagcgc aaactaggaa gctagccaca
       61 ggttgctcaa agcactgctt tgaggttgta gataagactg acgaagtcag ctcaaaatac
      121 tgttttgagg ttgcagatgg aagctgacgt agtttgaaga gattttcgaa gagtattata
      181 tgaaaaaatc ctctactcta ctaatccact tagaaaaaac gcatattatc aagtgttaaa
      241 aacttgctaa tatgcgtttt attatggaaa tgttgggttc attttctctt aaaattgatt
      301 ttttcttaga attctagctt aggtcatttt taaaattcaa attccgcaag aacatcttgc
      361 ccactggtga ccaattttgc tccttcttga atcaaatgat ggcaaccgtc tgatagtcca
      421 tctaaaatgc taccaggaat agcaaagaca tcgcgtcctt cttccattgc tcgctcacag
      481 gtaatgagac tacctgaacg catcttagcc tctgctacaa tcacaccacg acaaagtcca
      541 gcaatgatgc gattacgggc aggaaaatga aatttcagag gttgttcgcc aggtccatat
      601 tcacttagag ccagatggtc attgccgatg tagtcttgca agcgtttatt ggctttagga
      661 taaaacacat ccagtcctgt tccaatcact gcaatggttt ttccgccatt ctgaagagct
      721 gccatatgag ctgctgtgtc aatgcccttg gccagaccac tgacaataac cagttcattt
      781 tccaagcctt gaatgacttt ttcaactgac ttagctccct gtttgctaca agcacgactg
      841 cccacgaccg ctaccttcgg gaatttcagg aggtcaagat ttcccttgta aaataaaagt
      901 acaggcgcat catatatttc actcaaatcc caaggataac agtcatctaa aatagagaaa
      961 gatggaaatt tttgaaactc tttcgacaaa tgcgcatcgt ctatctgaaa ataacgttcc
     1021 ataaaaacgg ctggattacg gcaacctgat atatctgcaa tatcacccaa caaaagctcc
     1081 tgatcaacat tttcaccgta ttctagcact ttcaaaatct gttgattggt caaacctgat
     1141 ttttttaact tatagatttc atagtttgtg attttcataa ataactccat ttctttttct
     1201 actcatctat ctattcgtaa aagttcgtaa aaaatcaaaa aacatccgac tattttgtca
     1261 gatgttggaa tctttaattt tcgtttttaa gaatttcttc taatttacga tagtcttgga
     1321 cactatcgat ctcataaatg ctattgcctt ctaattcttc aacatagaca tctagctctt
     1381 tgatattatc cttaaccata ttgtcccaat agagatcaac aaattcacca cttgcataag
     1441 ccttgtcgat aaagctgaca atcttttctg cagttggagc atcccagaag gatacaccac
     1501 taaggatgcg acctgccttg ctatcaacaa taatgtcttg aaccttgtag tcatctccat
     1561 agaccaagaa ccattcgttg gtacaatctt cacgataaac actaaaataa gtcgaacgtg
     1621 tcaaatcatt gcggaacata tttttaaaga gataattgtc agcatcaata acatagctgt
     1681 tggccaattc ttcttttaca agatagagag agtaaaagtt attgtagtca gcgtatttat
     1741 cattgaaaac gaggcgaaca ccgtattttt cttttaaata atcgaattgt tctttaagat
     1801 aaccaacgat gatgatgatg tcattgattc ctttttcttt gagaaactca atttggtatt
     1861 caatcaaagg tttttgatta acctgaacca aggctttagg ggtattttca gtcataggac
     1921 gcaagcgagt tcccaatccc gctgctaaga taatggcttt cacacgaatc tcctttattc
     1981 tttaataata atataaactc cagcaatgac gacaagtgac gtcataatgg tcagcatatc
     2041 taatgatgtt cccaagaaaa gagctgcgaa caagacagtc catactacat agctcacgtt
     2101 caagcctgta gccttggctg gttgcaagcg attgatagcg atataataag ccaaatagga
     2161 aatcatatca aaggctacaa agacaatcat gagacctagc aattgtccat tggctactgc
     2221 aacaaatgac tgatgagaga agagcacaat cacaagatag gacaagaacg aagttacttg
     2281 acggattaag agggcttcga tttcactcaa ttcactttcc atggcaaaag agctaagaac
     2341 actctcactt ccccatgcaa tagcacaaac caaagcacaa agaatcccaa tgtagaaaga
     2401 attaacctgt tcaaccttat aggtctgggc aataatccct ccaataatca agacaatccc
     2461 aaatacagta tttttcgaaa tcttgtgctt caaaaagaag aaagccaata gaactgaaat
     2521 cgcagggtaa atagccgata cagatgaagc taaagaactt ccgatatact taactgcata
     2581 aagattggcc tgcataccga tagggcctgc tagcaaggct cctatgataa cactgacatt
     2641 gcgaatattt aagaaaattg agaggcgaac tttcccttct tttaccaaga gaaaagctag
     2701 taagataaag atgctcaaaa aatcatgagt tgcagccacc acaaagggtg acaaatctgt
     2761 aaaaatcgaa aagatataag cactaaccgt tagacccaag ccccagaaaa tacctgagag
     2821 aaggccaaaa ggaactccgt ttttactttt catctgaacc tccataagaa gccaaacctt
     2881 taatagctct ttggtaacga ttcacaccat agtcaccaaa atcttcacct tgctcttcct
     2941 tatagacagt ccatagactc caaatagtat cttgtaaaat tttataaata gcaatctttt
     3001 catgagaaac cggtgtttgg tcactctcat agtgagataa gaaagtttct tcctcttggg
     3061 aagtgaattc agactctaaa aagagggcag ccaaatccca cattggatca ttcattgatg
     3121 aatattccca gtcaatcaaa taaagtcgtc cttgaggtga ttcgataaag ttttcaggca
     3181 ccaaatcgat atgacaagat tttctgtcaa cacctaagtc agccagtctt ttctctaagg
     3241 agaagactgc atttctaaca gattcatagt tggcataagg aatttgttct tcaatcaagg
     3301 attcgtattt tttgatttct tcaaaaggag caaattctcc tcttaattcc ttagcagacg
     3361 tatgaatagt ttgtaatatt ggagtaattt tgtcgaactt ggtcttgatt gacgttgaat
     3421 caagcgtaat cgcagattcg atatactcat ttactttgat accagcttca atatcaaaaa
     3481 gataattttt tacatctaag cctaaatcct ttagtagttc aagattgtac ttttcatctt
     3541 gtcgattgat aagcttttct gtccctttac caaagaattt aacaatgtat tgcttatttg
     3601 ttgttttggc caaatagttt tgattggtca ttccacccag ttgttcaaca ctgaggactt
     3661 cctcttcttg actaagtaag gaagaaattt tttctttaat gattttctcc acaattaacc
     3721 tccagtactt atacttccca cttaaacact gttttaaagg ctgtgtttaa atcggttgca
     3781 aagacacgat gaatatcttt aatttctctt acaggttctt ctagataaag gatattttta
     3841 agacgattgg caaatttctt gacttccatc atttggatag cattttcaaa atcaatgcga
     3901 ccagaacgag atgacccaac caaaatcaag cccttttcta aggcatcgcg agtattgaga
     3961 ttgactttat attcgctaac tcccatcatg agaatcgttc cctgaggacg aatgtagcga
     4021 atcaagtcat taatagctgg tccagtacca tcaccaccac aacattcaaa agcatggtca
     4081 aaggccaaat cttcaggaat attatccgta atatagcatt ctttggcaaa tgagaagagt
     4141 tccaactttt cccaatgacg accaataacc acaatctctg cttctggcaa agtatagttg
     4201 ataatattgg caaccacaaa agctaaactt ccatctccaa taacggcgat ccgctcccgc
     4261 ttgctatgag caagagtcaa tagacgattc atagcgtgca tgcccacact gacaaactct
     4321 gtaatggctg caaccgtatc ttcaatagca tcataagcca ccacacgatc tttagggaga
     4381 gaaacaaact ctctcataaa gccatcaaat ccactagaca agaaatgggt ccctgtcatg
     4441 tagttttcat agaattcttc atcactctgc ataggagact gattgggaat catgacaact
     4501 ttttgaccaa cctcgtaggt tccggtcggg tcagaaatga cggttccaca tgactcgtga
     4561 atcattgcca ttggaagctt tttattcaaa atcttgggat cacgttttcc ctgatagtaa
     4621 cgctgatccg catgacagac agccatgtag ttgggacgga taaggatatg attctcttgg
     4681 tcaatagcct cttcctgata tttgacattg ataaacttag gcttagttag ttgataaatt
     4741 tgattaatca ttttactagt ctttctcaat catacttttt gcaatcttca aatctgttac
     4801 ggttgtaatc ttcagatttg agtattcacc tttggccaag gccacatctt ttcctttgat
     4861 cacaaagatt ttacatgcat ctgtcaagat ttccttctct tcatcagaaa gagatccata
     4921 aaggtccatg aagtccttgc aacggaatgt ttgaggtgtt tgtccttgat aaaggtgagc
     4981 acgatttgga atatctgtaa taaattgacc attggtactt tcaacgatag tatcaaccgc
     5041 ttctaccact gtgtccactg cgtcatgatt ttgggcaagt tggatattgt cctgaatcat
     5101 gcgaagtgta ataaatggac gaacagaatc gtgggtaaca acgatatcct ctggagtaag
     5161 cggacgataa gcatcaatgg cttcaatgat tttcttaata cttgtattgc ggtcagcacc
     5221 accctttgta atgatgatac gttccttata aagaggaaga tatttatcta caagatcttc
     5281 tgcatgagaa acccagtctc catgaacacc aactacaatt ttttcaatac ttggctccaa
     5341 gacaaatttt tcaattgtat gaatcaaaat aggtcgatca cctagctcta aaaattgttt
     5401 tggcaagtta ctgatcccca tgcgtgtgcc agttccaccg gcaagaattc ctgcataaat
     5461 catctatttt ctcctttgtc ttactaaaaa aacttattct ctctattata ccacaatcta
     5521 gtcctatcat aaaagaaata ctggtcctcc ccttctagaa taggagaatt atgcagtttt
     5581 attattcaaa gaaactagtc catttctagt aatgactagg aattctgtag caattactaa
     5641 taaatggctg cgaaatttta gagtccttca gaataatata ctttccttaa agaaaactta
     5701 aaacttaaga tttgaaactt caaagtgatt acttgactta ctgcccttca tttcttatta
     5761 gctaacttta tgatataatg aaaaacgagg taaattgaat gaaaagtata aaattaaatg
     5821 ctctatctta catgggaatt cgtgtcttga atattatttt tcccatccta actggaacct
     5881 atgtcgcgcg tgtcttggac cgaactgact atggttactt caactcagtc gacactattt
     5941 tgtcattttt cttgcccttt gcaacttatg gtgtctataa ctacggttta agggctatca
     6001 gtaatgtcaa ggataacaaa aaagatctta acagaacctt ttctagtctt ttttatttgt
     6061 gcatcgcttg tacgattttg accactgctg tctatatcct agcctatcct ctcttcttta
     6121 ctgataatcc aatcgtcaaa aaggtctacc ttgttatggg gattcaactc attgcccaga
     6181 ttttttcaat cgaatgggtc aatgaagctc tggaaaatta cagttttctc ttttacaaaa
     6241 ctgccttcat ccgtatcctg atgctggtct ctattttctt atttgttaaa aatgaacacg
     6301 atattgttgt ctatacactt gtgatgagtt tatcgacgct gattaactac ctgattagtt
     6361 atttttggat taaaagagac atcaaacttg ttaaaattca cctaagtgat tttaaaccac
     6421 tctttctccc tctgacagcc atgttagtct ttgccaatgc caatatgctc ttcacttttt
     6481 tagatcgcct cttcctcgtt aaaacaggga ttgatgtcaa cgttagttac tataccatag
     6541 ctcagcgaat tgtgaccgtt atagctgggg ttgtaacagg tgcaattgga gtgagtgtgc
     6601 ctcgtctcag ttactatctg gggaaaggag acaaagaagc ctatgtttct ctggttaata
     6661 gaggtagtcg aatctttaac ttctttatca ttccactgag ttttggactc atggttttag
     6721 gaccaaatgc catcctactt tacggtagtg aaaaatatat cggaggcggc atcttgacct
     6781 ctctcttcgc ttttcgtacg attatcctgg ccttagatac cattcttggt tcccaaattc
     6841 tctttaccaa tggctatgaa aaacgtatca cagtctatac agtctttgct gggctactca
     6901 atttgggctt gaatagtctc ctctttttca accatatcgt ggctcctgaa tactacttac
     6961 tgacaactat gttatcagag acttctctac ttgttttcta tatcattttc atccatagaa
     7021 aacaactcat ccacttggga catatcttta gctatactgt tcgatactct ctcttttcac
     7081 tttcctttgt agcaatttat ttcctgatta atttcatgta tcctgtggat atggtcatta
     7141 atttgccatt tttgattaat actggtttga ttgtcttgct atcagctatc tcttatatta
     7201 gtctacttgt cttcacaaaa gatagcattt tctatgaatt tttaaaccat gtcctagcct
     7261 taaaaaataa atttaaaaaa tcataggagt ttaaaatgaa acaactaacc gttgaagatg
     7321 ccaaacaaat tgaattagaa attttggatt atattgatac tctctgtaaa aagcacaata
     7381 tcaactatat tattaactac ggtactctga ttggggcggt tcgacatgag ggctttatcc
     7441 cttgggacga cgatattgat ctgtccatgc ctagagaaga ctaccaacga tttattaaca
     7501 tttttcaaaa ggaaaaaagc aagtataagc tcctatcctt agaaactgat aagaactact
     7561 ttaacaactt tatcaagata accgacagta cgactaaaat tattgatact cgaaatacaa
     7621 aaacctatga gtctggtatc tttatcgata ttttccctat agatcgcttt gatgatccta
     7681 aggtcattga tacttgttat aaactggaaa gcttcaaact gctgtctttc agtaaacata
     7741 aaaatattgt ctataaggat agccttttaa aagattggat acgaacagcc ttttggttgc
     7801 tccttcgacc agtttctcct cgttattttg caaataaaat cgagaaagaa attcaaaaat
     7861 atagtcgtga aaatgggcag tatatggctt ttatcccttc aaaatttaag gaaaaggaag
     7921 tcttcccaag tggtaccttt gataaaacaa tcgatttacc ctttgagaat ttaagccttc
     7981 ctgcacctga aaaatttgat actattttga cacaatttta tggagattat atgaccctac
     8041 caccagaaga aaaacgcttc tacagtcatg aatttcacgc ttataaattg gaggattagg
     8101 atgcaatatt tagaaaaaaa agaaattaaa gaaattcaac tagccctgct ggactatatt
     8161 gatgagactt gtaagaaaca tgatattcct tattttctca gttatggaac catgcttgga
     8221 gccatccgcc acaaaggtat gattccttgg gatgatgata ttgatatttc cctttatcgt
     8281 gaggattatg agcgtttact gaagattatt gaagaagaaa atcaccatcg ctacaaggtt
     8341 ctttcctacg atacatcttc ttggtacttc cataatttcg catcgatttt ggacacttct
     8401 actgttatag aagaccatgt taagtacaag cgtcatgata ccagcctttt catcgatgtc
     8461 ttcccaattg atcgatttac agacttgagc attgtcgaca agagctataa gtatgtggcc
     8521 cttcgtcaac tagcttatat caaaaaatca cgagcagttc acggtgatag caaactaaaa
     8581 gattttctta gattatgtag ctggtacgct ctccgatttg tcaatcctcg ctacttttac
     8641 aagaaaattg atcaactagt caaaaatgct gtaaccaaca ctcctcaata tgaaggagga
     8701 gttgggatcg gtaaggaagg gatgaaagaa atcttcccag ttgatacctt taaagaactg
     8761 attttaactg agtttgaggg ccgtatgttg cctgttccca aaaaatatga ccaattttta
     8821 acccagatgt atggcgatta tatgacacca ccatcaaaag aaatgcaaga gtggtatagt
     8881 catagcatta aagcttatcg caaaaactga ttgaggggga ttatacaaac tactaagata
     8941 gaggttattc aaaaacataa ttttagtaga aaatgaaata catattccca caataaaacg
     9001 catcatatca aggtttttga aaaaccttga tatgatgcgt tttataattt taa
//
LOCUS       DAVAAL010000070         9024 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVAAL010000070 DAVAAL010000000
VERSION     DAVAAL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.071"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(45..278)
                     /locus_tag="VQM60_001357"
     CDS             complement(45..278)
                     /locus_tag="VQM60_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET1081221.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(284..1276)
                     /gene="plsX"
                     /locus_tag="VQM60_001358"
     CDS             complement(284..1276)
                     /gene="plsX"
                     /locus_tag="VQM60_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET1081222.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1273..2043)
                     /gene="recO"
                     /locus_tag="VQM60_001359"
     CDS             complement(1273..2043)
                     /gene="recO"
                     /locus_tag="VQM60_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET1081223.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGTCLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2040..3209)
                     /locus_tag="VQM60_001360"
     CDS             complement(2040..3209)
                     /locus_tag="VQM60_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET1081224.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGECYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3358..4368
                     /locus_tag="VQM60_001361"
     CDS             3358..4368
                     /locus_tag="VQM60_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET1081225.1"
                     /translation="MSFLSKNGAGILVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDVGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILHFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
     gene            complement(4641..5078)
                     /locus_tag="VQM60_001362"
     CDS             complement(4641..5078)
                     /locus_tag="VQM60_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET1081226.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5163..7796)
                     /gene="polA"
                     /locus_tag="VQM60_001363"
     CDS             complement(5163..7796)
                     /gene="polA"
                     /locus_tag="VQM60_001363"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET1081227.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            8052..8402
                     /locus_tag="VQM60_001364"
     CDS             8052..8402
                     /locus_tag="VQM60_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET1081228.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            8617..8733
                     /locus_tag="VQM60_001365"
     CDS             8617..8733
                     /locus_tag="VQM60_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET1081229.1"
                     /translation="MVGVFRQQTLYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            8726..8959
                     /locus_tag="VQM60_001366"
     CDS             8726..8959
                     /locus_tag="VQM60_001366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081230.1"
                     /translation="MFSQLHMREEQVLLAQDYASETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
BASE COUNT         2536 a         2254 c         1700 g         2534 t
ORIGIN      
        1 atttctaaaa accatctact tccgttgact tggaactccg attgctattt tccttgaatg
       61 attttaacca catctcctac gttttggagt tggtcaattt cttcatcgct gatttcgata
      121 ctaaattcat cttccagagt caagataaac tccatcaagt caacagaatc cgcatccaaa
      181 tcgtctttca gactcaagga ttctgtcacg acaaagtcct ctccctgtcg ctcttggata
      241 atggtcacaa tacggtcaaa aatttctttt tctgtcatct cttttattct cctgaaaatt
      301 cacgcgcagt ctgggcaacc acgtctgttt ctagcatggt acggatctga cgaatcgtac
      361 tataaacagc cttggcatcg cttgagccat gagtcttgac aacaggtgcc ttaacaccaa
      421 acaagaccgc tccaccaaca tctgaataat tgagctgttt tttcaaacct ctgaggctgt
      481 ccttgagaag gagggcacct agtttcgctc gaagaccacc acctgtaata gctgtcttga
      541 gcaagcccat gattcccata gctgtccctt cgatggattt gagcacagcg tttcccgtga
      601 aaccatctgc cacaacaaca tctgcaacgc cattcatcaa atcacgcgct tccacgtttc
      661 cgataaagtt caaactttca tcagccgcca gtaattcata agtttcctta cgaagcgggt
      721 cgcccttgct actctctgtt ccgttgttaa gcaagccaac gcgtggttgc gcaatgccac
      781 gaacattctt ggcatagaaa gaacctagga cagcgtattg atggaggtgc tgggctgtat
      841 tttctgcatt agcaccgagg tcaagcatgt caaaaccttt cccatctaca gtcggcaatg
      901 ttgacataag tccaggacgg tcgatattct tgatacgacc cacgatgaag aatccagcag
      961 ccaacaaagc acctgtattc ccagccgaaa ggacagcgtc tgcttcacca tctttgacag
     1021 ccttggctgc caataccata ctggcatttt tcttattccg aatagctctc gtaggttcat
     1081 catccgaatc aatcttctca tccgtatgga taatgctgac gcgctctgtc gctgtcagat
     1141 attgcttgat tttagcttca tctccgtaga gttgaacctc gatatctgaa aagtcagata
     1201 gggcttgatt gacaccctca acgatggcct gaggtgcgta atcgcccccc atggcatcta
     1261 ctgcgatttt tttcatttct tttcctcttt tagtaattgt ccccagtctg ctagggaatc
     1321 aataaatttc tttgatttta ggtgaatccc aacgtactct tcatatagtt gatccataaa
     1381 ttggcgtagc tcttgcttga ttccaggctt gagcgaaatg gtctccaaag tctcaaaatc
     1441 aatagcttga aattgattga gcagataggg gatattggga ttgagatggc aacgtctctc
     1501 atcctcatga taatgctctg gacagagaca ggttccatat ttgaaagaaa agtcaaaagc
     1561 ctgaccaacc cgatggcaga agacacactc attaaaattg aggctgattc caaatcgagt
     1621 caaaatttga atttcaaaaa tattggtcaa aacctgataa tccaagcctg cttccatcaa
     1681 ctccaaagtc ttttgcaaaa aagcaaacaa gggagcatcc tgctgattgt cctgcaaact
     1741 agcatctgca agagctgcca cataggtcgc ataggccatg acaaagaggt cactattaat
     1801 cttgggaaaa gtcatgacct catgatagtc ttcgatgtaa ctgagtccgt catcattgat
     1861 tcgcaagaga aatcgtgcca gcaccaaggg ctgaataaca ggcgccagct tagactgacc
     1921 agcgtgtttg acaaaaaaca tgcgtttgcc aacctgctct gtaaaaattt tgacgagctt
     1981 gtcatcctca cgaaaattgc gattgtaaag caccaagcct tgactcgtga tagactgaat
     2041 catgcttctc tcatgtactc ctcaagtcgt ttcatggctt ctttgatagt ctccatgctg
     2101 gctgcataag ataggcggac gtagcattcc ccgtaacgtc caaaggctgc accagggata
     2161 aaggcaacgg ccttcttctg agcaaaatcc ttcagaaaag caaaggagtc ttgattgtag
     2221 cccgctggaa ttttagcaaa aatatagaag gcaccgtctg gtttgataat ctcaaaacca
     2281 agagcagtca ttttttcgat gatatagtcc cgacgctgga tatattcctt cttcatgggc
     2341 tccgcatcat ttttaccagc cgtcaaggct tctaccgcag catgttgcgc catggtattt
     2401 gcggcagtga ccaagtattg gtgactcttg attaactggg ctgtgaaggc cgcaggagcg
     2461 aaaatcagcc ccaaacgcca acctgtcatg gcatgcgatt tagacaaacc attgataata
     2521 atagcctggt ctctcaacat cgttcctaga gacacatggg cttcgcctgt gtaggtcaat
     2581 tctgagtaaa cctcatcaca gacaacaaaa atttcgtact tgcgtaaaac agctgccaag
     2641 gcctctaact gctctcgact gtaggtaatt cctgtcggat tggctggata gttgagaata
     2701 accgccttga gcttatcacc ctgctccaaa atggccttct ccaacatctc aggagtcaag
     2761 acaaaaccat tttcagtcgt atcaatctca acaatttctg ccccaactag attgacaatc
     2821 ggttcataac ccggataagc aggagctggc aagagcacct tgtctccctc ttccaaaata
     2881 gccgtcaaag tcgcagataa agcctctgtc gctccgatag tgaccaagat ttcattttca
     2941 ggagcatagt ccagttggta cttttcctta acaaaatcac tggccgcctg acgtagagtc
     3001 agcagaccac tcatccctgt atagtaggat tggttctgat caatcgctcg cttggccgcc
     3061 tccttgacat ggtctggcgt tgtaaaatca ggttccccca aggtcaaacg caagacccca
     3121 ggaatctccg aaataacctg gtcaaactga cgaatcaacg aaacttgaat tttatctaac
     3181 tgtttattaa agcgcttagt taagtccata gatacctcct actttcaaaa cgtatctcta
     3241 ttatactaca aaagcccccc gaccgcaaaa atacattaga gaatagcttt ccactttcta
     3301 ttttactttt cctgtttaca ggtctataat ggtaacagat tctatttgga ggtttttatg
     3361 tcatttctat caaaaaatgg agcaggcatc ttggtctgcc ttctcatttc tatcgtatct
     3421 tggtacttag gaggattctt ccctgtgatt ggcgcgcccg tttttgcgat ttttatgggc
     3481 atgctcctac atccctttct ctcgtcttat aaacaactgg atgttggatt gacctttagt
     3541 tccaagaaat tgctccagta tgccgttatc ttgcttggtt ttggtctcaa tatctctcaa
     3601 gtcttcgcag ttgggcaatc ttcactccct gtcatccttt ccaccatttc aatagccttg
     3661 attgttgcct acctcttcca gcgcttcttt gccctggata caaaactggc taccttgatt
     3721 ggagtaggtt cttctatctg tgggggttct gccattgcag cgacagcgcc tgttatccat
     3781 gctaaggaaa aggaagtagc ccaagccatc tccgttatct ttttcttcaa tgtcttggct
     3841 gcgctcatct ttccaaccct cggcacctgg cttcatctat ccaatgacgg cttcgccctc
     3901 tttgcaggaa ctgcggtcaa tgatacttcc tctgtaaccg ccacagctag cgcttgggac
     3961 agtctctacc agaccaatac cctcgaatcc gcaaccattg tcaaactcac gcgaacccta
     4021 gctatcatcc ccatcactct ctttctctcc tactggcaaa gtcgcgagca aaaaaataag
     4081 caaggcttac aactgaaaaa agtcttccca ctttttatcc ttcacttcat ccttgcatcg
     4141 ctcctaacca cccttctgac ctctctcggt gtgtctagta gtttctttac ccctctcaaa
     4201 caactctcta aattcctcat tatcatggcc atgagtgcca tcggtctcaa aactaatctg
     4261 gtcgctatgg tcaaatccag tggaaaatcc attgttctcg gagccgtctg ctggattgcc
     4321 atcatcctca ccagccttgg tatgcagacc cttatcggca ttttctaata ctcttcgaaa
     4381 atctcttcaa accacgtcaa cgtcgccttg ccgtatatat gttactgact tcgtcagttc
     4441 tatctgcaac ctcaaaacgg tgttttgagc tgacttcgtc agttctatct gcaacctcaa
     4501 aacggtgttt tgagctgact tcgtcagttc tatctacaac ctcaaaacag tgttttgagc
     4561 agcccgcggc tagtttccta gtttgctctt tgattttcat tgagtataac acaaaaggta
     4621 gcccatcagc tacctttttc ttatgcttcc tcaatcaagc gagtatgttc tctcttgata
     4681 cagcgattca tcacgatatc atcacatcca ccatcacgca aaatctcttt cgcttctaaa
     4741 ctttcaagtc ctagctgtgc ccaaaaaatc ttggcatcag ctttgagaaa atcacgcgcc
     4801 acatcgggca gaaattcact gcgacgataa acattgacaa tatctacagg aaaaggaatt
     4861 tcagcgaggc tagcataagc cttttcaccc aagatttcgc cacctgccgc cttgggattg
     4921 actgggatga ttttatagcc ccgagcctgc atttcctttg ttactcgatt gctggttgtt
     4981 tcttcacggt cagacaaacc caccacagca agggttttac tcgttgcgag atactgacga
     5041 atcacgccat cacttggatt gataaattct tgactcatag aaatcctcct ttttcatcag
     5101 tatagcacat tttgaaaagg tttgcagaat tatactacaa aaaaggagga ctagccccct
     5161 ttttatttag cctcgtacca ggttgcccct tcattctcat ctgcgataag aggaacactg
     5221 agttgaatgg cttcttccat ggtttgtttc accaattttt tcatctctac caattcagat
     5281 ttaggcactt caaggacgat ttcatcgtgc acttgtaaca gcatcttagt ctgataacca
     5341 cctgcaacca aggctttatc cagctgaatc atggcaatct tgagaatatc tgctgccgaa
     5401 ccctggatag gtgagttgat agcagttcgc tccgcaaaac cacgaatatt gaagttgcgc
     5461 gaattgatat ctggcaactc acggcgacgc ttaaagaggg tctctacata gcccttatca
     5521 cgcgcctccc gcaccacttc atccatgtag tttttaatac ctggaaaacg ttcaaagtag
     5581 gtatcaatgt aggctttggc ttccttacga ctaattccca aattattaga caagccaaag
     5641 tctgaaatcc cataaaccac tccaaagtta actgccttgg cattgcgacg gtcgtttgca
     5701 gtcacatcat caggacgctc aatgccaaag acccgcatgg ctgtcgaagt atggatatct
     5761 gccccctctt ggaaggcctt aatcaagtgc tcatccttag aaatatgcgc caaaacgcgc
     5821 aattcaatct gtgaatagtc agagctgagt agcacactat cctcccactc tggcacaaaa
     5881 gccttccgaa tcaagcgccc ctgttccaat cgggcaggaa tattttgcaa gtttggatcc
     5941 acactagaca aacgcccggt ctgggtcaaa tcctgcacat agcgagtatg aatctttcca
     6001 tcagccaaaa tccagtcctg caagccaatt acataagtag attgaatctt agcaatttga
     6061 cggtaatcca ggattttctt aacaatcgga gcaataggag cgagacgctc caacacatcc
     6121 actgctgtcg aataacctgt cttggttttc ttagtgtatt ctagaggaag tcccaatttc
     6181 tcaaagagaa gcacgcccaa ctgcttaggc gagttgacat taaactcctc accagccagc
     6241 tcgtaaatct cttgagtcag tttttcaatg acaagctcat tttcagcctg catctcaagc
     6301 aaggtctctt tcttgaccat aatcccagca atttccatct tggcaaggac aaaagccaga
     6361 ggttgctcca tatcataaag aagctctaat tgcccatttt cgctgagttt ttcaagtaaa
     6421 ataggctctg tttctaccaa aacagcaagt ttacaagcta agtgttccaa gaatttctca
     6481 cgttcaggaa tggccttttt aacaccctta ccgtagaaag tttcatcatc aaccaagtaa
     6541 gtctgaccat aaagactagc gatggtcgca atttcattgt cctccacagt cgaaaggagg
     6601 tatttagcca aacggatgtc aaaagcaggc gcctgcaaat ccacaccaaa acgttgcaaa
     6661 agaactttaa ccttcttaaa gtcataaact ctcagagatg ttttttctaa gaaatccttg
     6721 aaaatcgggt cttgcaacag ctcaagcttg tctgtggcat agagcttatc cccacaagac
     6781 caggcaaatc caaccaaatt atccgtatgg taattctcac caaaaagctc aaagtggaag
     6841 atagactctt cactcagcat atcttgactg atttggtcaa caatagtaaa atccaaactc
     6901 tcagacacat cagctgacga cacatttaaa gcctgcttta gctgtttgaa gcccatctca
     6961 tcgtagaatt tcccaagatt ttcaacatct ggaccactat agaccaagtc ctctaaacca
     7021 atcgcaatcg gtgccttggt atcaatggtc gctagtgttt tagataaaaa ggcctgttcc
     7081 ttgtcattga tgagattttc cttcatctta gaagtcttca ttccatcaat attttcataa
     7141 atcccctcaa gcgaaccatg ctccagcaag agcttaatac ccgtcttttc accgactttg
     7201 gtcaccccag ggatattatc cgacttatca cccatgagcg ccttgagatc gataaactga
     7261 gctggtgtga ggcccatttc ttccatgagg taatctggcg taaaggcctc aaactcagcc
     7321 acacctttct tggaaatttc aaccaccgta tgctcatccg tcagctgaat caaatccttg
     7381 tccccactga caatggtaat atcaaaacca tcctgctctg ctagcttatc cagcgtccca
     7441 atgatgtcat ccgcctcata ctgagccagc tcatagtgac gaatccccat atgatccagc
     7501 aactcacgaa tgaaaggaaa ttgctcacga aactcatcag gagtcttggc ccgaccaccc
     7561 ttatagtccg catacatctc tgtccggaag gtcgtctttc ccgcatcaaa agccaccaaa
     7621 atatgactcg gctcaacccg ctccaacaaa tgactcaaca tcaactgaaa accataaatc
     7681 gcattggtat gcaaaccagc cgcattctta aaacggtcca actgctgata cagcgcaaaa
     7741 aacgcccgaa aagctacaga agacccatca atcaataata attttttctt atccatacac
     7801 ccattataaa ggaaagaatc aaaaaatacc attgggaaga gctagagcaa gtatttttca
     7861 aactttttcc gaataaatag atagagccag agaatttagt aaacctagat ttaaaaatgt
     7921 gctataatat agtatattga atctataata gtacaccttg actgctaaaa tatttctata
     7981 aattaatttg actttcctga tagagttatt cacatcttat ttcaactcac tatagaagga
     8041 ggaataggag gattctcaga catccgggca tcagcccaac taatgatttg attgctaaga
     8101 aaatattcag caatccagaa atcacttgtc aatttattcg cgatatgctg gacttgccag
     8161 caaaaaatgt gaccattttg gagggaagcg atattcacgt attactctcc atgccttact
     8221 cggtgcagga tttttatacc agtatagacg tcttggcgga gttggataac ggtactcaag
     8281 taattattga gattcaagtc catcatcaga attttttcat caatcacttg tgggcttacc
     8341 tgtgcagtca ggttaatcaa aatcttgaaa aaattcgtca gcgagaaggt gatactcact
     8401 agatctacaa acacatcgct cctgtttacg tcattgctat cgtggatagt aattatttct
     8461 cagatgacct ggcttttcat agctttagta tgcgcgaaga cacaacaggt gaggtattgg
     8521 cgattaccaa caatggacag gaaaaccatc tggttaagat ggcattcttg gaattaaaaa
     8581 atacagagaa accagcaaag acaaggttcg caagccatgg ttggagtttt tcggcaacaa
     8641 accctttacc cagcaaccgc aacgagccat tacccaagca aatcaactgc tggactacaa
     8701 gagctggtcc gaggaggaca ggaaaatgtt tagtcaacta catatgcgag aagaacaagt
     8761 cttgttagca caggactatg cctcggaaac tgctagggct gaaggccttg aacaaggact
     8821 agagcgtggg aaagttgaag gaagggcaga aaggaaactt tttgccttcc tagacatagt
     8881 acgccaaggt cttctgactt ctgaggttgc cagccagcaa ttaggtatgt cagtatctga
     8941 atttgaggca ctgttgtaaa atggctccat aatatccata ggggatttac ccactatgga
     9001 tattatggag cctattttgt gtaa
//
LOCUS       DAVAAL010000071         8936 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVAAL010000071 DAVAAL010000000
VERSION     DAVAAL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.072"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..723
                     /locus_tag="VQM60_001367"
     CDS             <1..723
                     /locus_tag="VQM60_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET1081231.1"
                     /translation="VIQRHGALLLVENGQLLYVELPKEGVNVHDFFETSKVRETLLIA
                     TRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEVAETGMTFEENARLKAETISQLTGKM
                     VLADDSGLKVDVLGGLPGVWSARFAGVGATDRENNAKLLHELAMVFELKDRSAQFHTT
                     LVVASPNKESLVVEADWSGYINFEPKGENGFGYDPLFLVGETGESSAELTLEEKNSQS
                     HRALAVKKLLEVFPSWQSKPSL"
     gene            699..1220
                     /locus_tag="VQM60_001368"
     CDS             699..1220
                     /locus_tag="VQM60_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET1081232.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            1217..1678
                     /locus_tag="VQM60_001369"
     CDS             1217..1678
                     /locus_tag="VQM60_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET1081233.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDSEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            1669..2409
                     /gene="xerD"
                     /locus_tag="VQM60_001370"
     CDS             1669..2409
                     /gene="xerD"
                     /locus_tag="VQM60_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET1081234.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISSCNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            2409..3137
                     /locus_tag="VQM60_001371"
     CDS             2409..3137
                     /locus_tag="VQM60_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET1081235.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLESKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            3156..3725
                     /locus_tag="VQM60_001372"
     CDS             3156..3725
                     /locus_tag="VQM60_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET1081236.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            3712..4434
                     /locus_tag="VQM60_001373"
     CDS             3712..4434
                     /locus_tag="VQM60_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET1081237.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            4434..4676
                     /gene="yidD"
                     /locus_tag="VQM60_001374"
     CDS             4434..4676
                     /gene="yidD"
                     /locus_tag="VQM60_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET1081238.1"
                     /translation="MKRILIALVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(4901..5866)
                     /locus_tag="VQM60_001375"
     CDS             complement(4901..5866)
                     /locus_tag="VQM60_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET1081239.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFAKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(5962..6714)
                     /locus_tag="VQM60_001376"
     CDS             complement(5962..6714)
                     /locus_tag="VQM60_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET1081240.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(6711..7667)
                     /locus_tag="VQM60_001377"
     CDS             complement(6711..7667)
                     /locus_tag="VQM60_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET1081241.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFILANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(7657..8616)
                     /locus_tag="VQM60_001378"
     CDS             complement(7657..8616)
                     /locus_tag="VQM60_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1081242.1"
                     /translation="MKLSYYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKCYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
BASE COUNT         2541 a         1791 c         2140 g         2464 t
ORIGIN      
        1 gttattcaac gtcatggggc cctgctcttg gttgaaaatg ggcaactctt gtatgtagaa
       61 ttgcctaaag aaggggtcaa tgttcatgat ttctttgaga caagcaaggt cagagaaacc
      121 ttgttgattg cgactcgtaa cgaaggtaaa accaaggaat tccgagctat ctttgataag
      181 ttaggctacg atgtggaaaa tcttaatgac taccctgacc tgcctgaagt agcagaaaca
      241 ggtatgacct ttgaagaaaa tgcccgcctt aaggcagaaa ccatttctca attaacgggc
      301 aagatggttt tggcagatga ttctggtctc aaagtcgatg tccttggtgg cttaccaggc
      361 gtctggtcag ctcgtttcgc aggtgtggga gcaactgacc gtgaaaataa tgccaaactc
      421 ttgcacgaat tggccatggt ctttgaactc aaggaccgct cggctcagtt ccacacaacc
      481 ctagtcgtag ccagcccaaa taaggaaagt ttagttgttg aagcagactg gtcaggttat
      541 attaactttg aacctaaggg tgaaaatggc tttggctatg atcccctctt ccttgtagga
      601 gagacaggtg agtcatcagc tgaattaacc ctggaagaaa aaaatagtca atctcaccgt
      661 gccttagccg ttaagaaact tttggaggta tttccatcat ggcaaagcaa accatcattg
      721 taatgagcga ttcccatggc gatagcttga ttgtggaaga agtccgtgat cgctatgtgg
      781 gcaaagtcga tgccgttttt cataacggcg attctgaact acgtccggat tctccacttt
      841 gggagggcat ccgcgttgtt aaagggaaca tggacttcta cgccggctac ccagaacgtc
      901 tggtgactga gcttggttcg accaagatta tccaaactca tggtcacttg tttgacatca
      961 atttcaactt tcaaaagttg gactactggg ctcaggagga agaggccgct atctgcctct
     1021 atggtcactt gcatgtgcca agtgcttgga tggaaggcaa gatcctcttt ctaaatccag
     1081 gttctatcag tcaaccacga ggtaccatca gagaatgtct ctatgctcgt gtggagattg
     1141 atgatagtta cttcaaagtg gactttttga cacgagatca cgaggtgtat ccaggtttgt
     1201 ccaaggagtt tagccgatga ttgccaagga gtttgagact ttcttgttgg ggcaggagga
     1261 aacttttttg acccctgcta aaaatctagc tgtgttgatt gatacccaca atgcggatca
     1321 tgcgaccctc ttgctcagtc agatgaccta tacccgtgtt cccgttgtga cagatgaaaa
     1381 acagtttgtt gggacgattg gactcagaga tattatggct tatcagatgg agcatgactt
     1441 gagccaagaa atcatggcgg atacggatat cgttcatatg acaaaaacgg acgtagcggt
     1501 tgtttcgcct gatttcacca ttacggaggt cttgcacaag ctagtagatg agtccttctt
     1561 accggttgtg gattcagagg gtattttcca agggattatt acgcgcaagt ccatcctcaa
     1621 ggccgttaat gccctcttgc atgactttag taaggaatat gagattcgat gccaatgaga
     1681 gacaggattt cagccttttt agaggaaaag cagggcttgt ctgtcaattc caagcagtcc
     1741 tataagtatg atttggagca atttttagac atggtaggtg agcggatttc tgagaccagt
     1801 ctcaagattt accaagccca gctagccaat ctaaaaatca gcgcccagaa gcgaaagatt
     1861 tcgtcctgta accaatttct atactttctc tatcaaaaag gagaggtgga cagcttttac
     1921 cgcttggaat tagccaaaca agctgaaaag aagacggaaa agccagagat tctataccta
     1981 gactcttttt ggcaggaaag cgaccatcca gagggccgct tgctagcgct cttaatccta
     2041 gaaatggggc tcttgcccag tgagatttta gccatcaagg ttgcggacat caatctggat
     2101 tttcaggtgt tgcgaatcag caaggcttcc caacagagga ttgtcaccat tcccacggcc
     2161 ttgctttcag aattggaacc cttgatgggg cagacctatc tttttgaaag aggagagaaa
     2221 ccctattctc gtcagtgggc ctttcgtcag ttagaatctt ttgtcaagga gaaaggtttt
     2281 ccatccttat cagctcaagt cttacgtgaa cagtttattc taagacaaat agaaaacaag
     2341 gtcgatttgt acgaaattgc aaaaaaatta ggattaaaaa cagtcctgac cttagaaaaa
     2401 tatagataat ggatattaaa ttaaaagatt ttgaaggacc cttggacttg ctcttgcatc
     2461 tggtttctaa gtaccagatg gatatctacg atgtgcccat tacggaagtc atcgaacagt
     2521 atctagccta tgtctcaacc ctgcaggcca tgcgtctgga agtgacgggt gagtacatgg
     2581 tcatggctag tcagctcatg ctgattaaga gtcgtaaact ccttccgaag gtagcagaag
     2641 tgacagactt gggggatgac ctggagcagg acctcctctc tcaaatcgaa gaatatcgca
     2701 agttcaagct cttgggtgag cacttggaat ccaagcacca agaacgggcc cagtattatt
     2761 ccaaagcgcc gacagagttg atttacgaag atgcggagct tgtgcatgac aagacgacca
     2821 ttgacctctt tttggctttt tcaaatatcc tagccaagaa aaaagaggag tttgcacaaa
     2881 atcacacgac tatcttgcgg gatgagtata agattgagga catgatgatt atcgtgaaag
     2941 agtccttgat tggacgagat caattgcgct tgcaggattt gttcaaggaa gcccagaatg
     3001 tccaagaggt catcaccctc tttttggcaa ccctagagtt aatcaaaacc caggagctga
     3061 tcctcgtgca agaggagagt ttcggagata tctatctcat ggaaaagaag gaagaaagtc
     3121 aagtgcctca aagctagact tgatagagag gaaagatgag tactttagca aaaatagaag
     3181 cgctcttgtt tgtagcgggt gaagatggga ttcgggtccg ccagttagct gaactcctct
     3241 ctctgccacc gacaggcatc cagcaaagtt taggaaaatt agcccagaag tatgaaaagg
     3301 acccagattc cagtttggct ttgattgaga caagtggtgc ttatagattg gtgaccaagc
     3361 ctcaatttgc agagattttg aaggaatact ctaaggcgcc tatcaaccag agcttgtctc
     3421 gggctgccct tgagaccttg tccattattg cctacaaaca gccgattacg cggatagaaa
     3481 ttgatgccat ccgtggagtt aactcgagtg gagccttggc aaagttgcag gcttttgacc
     3541 tgataaagga agacgggaaa aaggaagtat tggggcgccc caacctctat gtgactacgg
     3601 attatttcct agattacatg gggataaacc atttagaaga attaccagtg attgatgagc
     3661 ttgagattca agcccaagaa agccaattat ttggtgaaag gatagaagaa gatgagaatc
     3721 aataagtata ttgcccacgc aggtgtggcc agtaggagaa aagcagaaga gctgattaag
     3781 caaggcttgg tgacggttaa cggccaagtg gtgcgtgaac tagcaaccac tatcaagtca
     3841 ggcgacaagg tcgaagttga aggtcaacct atctacaacg aagaaaaggt ctactatctg
     3901 cttaacaaac cacgcggtgt catttccagt gtgacagatg ataagggtcg caagacggtt
     3961 gtcgacctct tgcccaatgt caaagagcgt atttaccctg tgggtcgttt agactgggat
     4021 acatcaggag tcttgatttt gaccaatgat ggggacttta cagacgagat gattcaccct
     4081 cgtaatgaga ttgacaaggt ttatgtcgcg cgtgttaaag gtgtggccaa taaggacaat
     4141 cttcgcccct tgacccgtgg tcttgagatt gatggtaaga aaaccaagcc agctgtttat
     4201 gaaattctca aagtggaccc agtcaaaaat cgctctgtgg tgcagttgac catccatgaa
     4261 gggcgtaacc atcaggttaa aaagatgttt gaagctgttg gtctccaagt agataagttg
     4321 tctcggactc gtttcggaca cctagacttg acaggactcc gtccaggaga atcccgtcgt
     4381 cttaataaaa aagaaatcag ccaactacac accatggctg taactaagaa ataatgaaac
     4441 gaattttaat agcgcttgtg cgcttttacc aacgttttat ctcaccagtc tttccaccct
     4501 cttgtcgctt tgagctgact tgttccaact acatgattca ggctattgaa aaacatggtt
     4561 ttaagggggt attgatgggc ttggctcgga ttttacgttg tcatccctgg tcgaaaacag
     4621 gtaaggaccc cgttccagac cacttttccc ttaaacgaaa tcaagaaggg gaatgaggtg
     4681 gggtaaatag atttcaaaat gataaaaacg catcctatca ggtttgagtg aacttgatag
     4741 gatgcgtttt agaatgtcaa aattttatac tcttcgaaaa tctcttcaaa ccgcgtcagc
     4801 tttcatctgc aacctcaaaa cagtgttttg agcaacctgc ggctagtttc ctagtttgct
     4861 ctttgatttt cattgagtat taaattgagt ttgaagtggc ttatttcaaa gctttttgta
     4921 tgtcttcaat catgagtttt gttgattcaa gtccgcctcc gcttagatac cagaggtctg
     4981 gtgttagttg gataatctta ccatttttag cagcaggtgt ttcagcgata agggcatttt
     5041 ctaggacacc gtcgttgcta gagttgtccc caccgatggc aagggtacgg ttgatgacaa
     5101 agaggatgtc agggttgatt tctttgacac tttcaaagct gacttcttgt ccgtggcgtg
     5161 agtcttcaaa ttttgtatca gttggtttga atttcaaggt ttggtacaag aaagagaaac
     5221 gagatttggc accaaaggct gccatttttc cttcattaag gaggatcgca agggcttttt
     5281 tgtcagagct ttcattttta gtagcgactt cttggatgct cttgtctagc ttggccaatt
     5341 cttccttggc tttctgtgta ccagtttcgc cgaaggcact tgctaaggat tcgatattag
     5401 ccttggtaga agtccagtag tcgtccttgc ttgcttggaa gagaacggtt ggggcgattt
     5461 ctttgaattt gtctacgaat ttttgtgtac gtggtgaagc gataatcaaa tcaggctcaa
     5521 gggcggcgat agcttctaaa tcaggttctt tcatagaacc aacatttttg acagttccca
     5581 ctaggtcttt tagataagtc ggaacagttt ttgtaggcat tccgacgata ttttttgcaa
     5641 atcctaaagc gcgaatagta tccgcagcgc cgaggtcaaa ggtcacaatc ttttcaggaa
     5701 ctttggaaag tttgacctcg tccagtgaac ttttaatggt tacctctgtt ggagcagagc
     5761 tactggtctc tgtctgacta gtgcttgagt ttgtactaca tgcaccaagt aggagcaaga
     5821 agctggccac tagggcagtg aaataaagtt taagggatgt tttcataatt tctccttttt
     5881 aaaatgtgat aacgatttag ggagtctctt aatcttattg actaagagac tgaaggttct
     5941 ctaacttgag cttttatgtt actagctata gatacagatc tttttgtcat tgatatcagc
     6001 tagcgtgatg ggaatctcat aaagttgact gagcaggtca gcctgcatga tttgatcggt
     6061 tcttcccttg ctaaagacct ggccgtcctt gaaggcgaca atttcatctg catactgact
     6121 ggccatgttg atatcgtgga ggacgatgat aatggtcttg ccgagttcct ccaccagtcg
     6181 tcgaagaatc tgcatcatgc tgacgctttg cttgatatcg agattgttga gtggttcgtc
     6241 cagcaagata aagtccgtat cctgggccag taccatagcg ataaagacgc gctggagttg
     6301 cccccctgac aggctattga tgtagcggtc ttttaagttg gtcagttcta aatagttcag
     6361 agtttctcgg attttttccc agtcttctga tctaagtcga cctcggctgt agggaaaacg
     6421 tccaaaactg accagttctt caacagtcaa tttggcttgg taattgattt tctgttttag
     6481 gatggttagt tcttgggcca gttcttgcga attccagctc tcgatttcac gtcctttgat
     6541 actgagaact ccctgatctt tcttggttag cctgctcatg atggagagga gagtcgattt
     6601 tccagcacca tttggaccaa taaaggctgt cagtttttga ggactgactt caagcgaaat
     6661 gccttgcaaa atatcctgtt tttgaatgga tttgtcaatg ttttccagtt tcactgacga
     6721 gacctcctat atagtaagat aaagaataag aagccaccca cactctcaat gatcatactg
     6781 atacgaattt ccagtgcaaa gactcgttca atcaaggctt gccccaaggt taagctaata
     6841 aatccaacca gaatggccac tataaagagt aacttgtgct gatagtcttt gacaatcagg
     6901 taggtgaggt tggccagtat aaagccgaag aaggccatag gtcctaccaa ggcagtggcc
     6961 gttgaggtca aaagcacgat tccccagagg agctctttct gttctttttc aacatcgagt
     7021 cccaatatct gagccgtttc tctttgcagg tgcaagacat ctagaacgac tgcttttcga
     7081 aagaaaaaga ttgtcaaagc gaggatgatc agagaaccga tggctaggat ggaagtgttg
     7141 agatgttgaa aggaggcaaa aagactattt tgcagtttat cgtattcgtt tggatccatt
     7201 aggacttgaa ggaaggtgct gatatttcga aagagacttc tgagcgctag acagatcagc
     7261 aggatgaaga ccaggtcttg cttcatcagt gtcttcaagt aaccttgtaa ggcgagaaag
     7321 aagagggact ggacaagaag taagactagg aattctaaga taggggattt gccaagttga
     7381 agaaacttgc tttcaaaaac cagtagtagg gtttgtagta ggacgtagaa ggattcaatt
     7441 cccaaaatac taggcgtcag gaagcgattt tccgtcaggg tttgaaaact aatggtcgaa
     7501 atcccagtcg cgatggctac caagagataa acgatgatct tttgggaacg caacttccaa
     7561 gcaaaggctg acaagtgagt gatgggccaa aagtagagaa gacaagctcc gatggcaaga
     7621 ataatgagaa tccagaagag cttggtatgt ttgcttttag tctgcatctt ttcgtccccc
     7681 tctccagaga agtaggataa agacgagact accgatgatt cctagcaaga gactgacaga
     7741 caactcatag ggcctaatca gaactcggga taggatatcg caagccagaa ctagattggc
     7801 accaaccagt gcgaccatga gtttggtttg acttagatta tctccatagc acttgcgaac
     7861 aagattggga acgataactc cgagaaatgg taggccaccc acggtaatca tggtgacgct
     7921 tgtcgttagc gccaccagaa agagggccag tttttcaagt agggagtagg aaatccccaa
     7981 actctcgctg gtttctttcc ctagattcat gatggtgaag gtttgggata atttccaaac
     8041 ggttatcagg atgatgaggc ctaagaagag ccactcatac tgatgggtct gaatcatgga
     8101 gaaggagccc tgggtccagg cagtcatact ctgaaccaga ttgaaacgat aggcgataac
     8161 ttctgtgact gagccgataa tcccgctata gatgatccca atcagaggca acatccacct
     8221 ttcctttaca gtaaaaatgg tcataaaggc taggaagaag agggtgaata cgatggatga
     8281 aacaaaagcg aagagcatct tttgggtcag actagccgat ggaaagacaa aaaggctcag
     8341 caccattccc agtttggcgg cttcagtcgt tccaactgta ctcggtgcag caaactgatt
     8401 ttgggtaata gtctgcatga gaaggcctgc catactcata ctagaggcag tcaggagaat
     8461 actgatagtt cttgggagac gggactcttg aaagaggagc caggtctgct ggtcgaaatc
     8521 aaatagcttt ccccatgaaa aatcactggt cccaatgcta atagagagaa agactaggag
     8581 tagaagtaag ccaattaaat aataagaaag tttcataccc cgtcctttca tgtagatttg
     8641 gtatcgaaag atatctgcgg atataaatgt aacattattt ttctaatctg tcaataaaat
     8701 ttctgacaat ttaataaata caacaaggag agagcaacaa gactttctcc tttgttatcc
     8761 tattctaaaa tgtttttacc ttaatctgat aaaataatat cttcgaggga gtagctagcc
     8821 gtccaatcaa gatattgttt agcttttgaa gcatctgcta ggacactggc tgggtcacta
     8881 gcacgtcgag caacaatctc gtgtgggatt ttttaattta gtaattcttc agcagt
//
LOCUS       DAVAAL010000072         8881 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVAAL010000072 DAVAAL010000000
VERSION     DAVAAL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.073"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(232..1839)
                     /locus_tag="VQM60_001379"
     CDS             complement(232..1839)
                     /locus_tag="VQM60_001379"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET1081243.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2229..2816)
                     /locus_tag="VQM60_001380"
     CDS             complement(2229..2816)
                     /locus_tag="VQM60_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET1081244.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2944..3393)
                     /locus_tag="VQM60_001381"
     CDS             complement(2944..3393)
                     /locus_tag="VQM60_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081245.1"
                     /translation="MCFVPYYKVNYCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3398..3787)
                     /locus_tag="VQM60_001382"
     CDS             complement(3398..3787)
                     /locus_tag="VQM60_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HET1081246.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRGGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3814..4440)
                     /locus_tag="VQM60_001383"
     CDS             complement(3814..4440)
                     /locus_tag="VQM60_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET1081247.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVGFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4454..5017)
                     /locus_tag="VQM60_001384"
     CDS             complement(4454..5017)
                     /locus_tag="VQM60_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET1081248.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5182..5416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5470..5973)
                     /locus_tag="VQM60_001385"
     CDS             complement(5470..5973)
                     /locus_tag="VQM60_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET1081249.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6166..6996)
                     /locus_tag="VQM60_001386"
     CDS             complement(6166..6996)
                     /locus_tag="VQM60_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET1081250.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6981..8663)
                     /locus_tag="VQM60_001387"
     CDS             complement(6981..8663)
                     /locus_tag="VQM60_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081251.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQAAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNLSIS
                     KGQVQLQNELAKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
BASE COUNT         2679 a         2042 c         1622 g         2538 t
ORIGIN      
        1 aatccagttc ctaaaacttt gctagaaatt gatttggctt tccgaattaa tttgttcaga
       61 ttttatttca tttcaatata ctgataaatc cattcactag cacagggcaa gagggagtcg
      121 ctattaattt caacggtagc aggtacatac agaggactcc tacctcagat atactgcaat
      181 atcattgtat gcaaaaaacc tctgagattt ttctcaaagg ttctgatttt gctaattgct
      241 gttctcaacc gctgcagtaa caaaggcagt gtagagttct tctggtcggt ttggacggct
      301 tgacagttca gggtgatact gacaagctac aaagaattta ttttcaggaa tttccacgat
      361 ttctaccaaa cgattgtctg gagaaactcc tgaaaagaca aaacctgctg cctcaaactg
      421 ctcacggaag gcattattaa actcataacg gtgacggtga cggcgttgca ccacttcttg
      481 attgtgataa gcagcagcag ccttagagcc acgtttcaac ttagacggat aaagtcccaa
      541 acgaagggtt ccacccatat cctcaatatc aatctgatca cgcatgatat caatgatagg
      601 gtattttgtt tctggtgcaa gctctgcaga attggcacct tcaagaccta aaacgtgacg
      661 agcaaactcg atacatgtca actgcattcc caagcagact cccaacattg gaacatcatt
      721 ttcacgcgca tagcggatgg cttggatttt cccttctgta ccacgttgac caaaaccacc
      781 tggtacgatg atcccgtccg catcagacaa gagttctgct acattctctg ctgtcacatc
      841 attggcattg acccaattga ttttaacttc tacatcattg acatagccag agtgtttcaa
      901 ggcttcgacc actgagatat aggcatcttg caactccaca tacttaccaa caagggaaat
      961 cttaacttgt ttcttgaggt tcatgacctt gtccaccatg gctgaccatt ctgtcatatc
     1021 cgctgctggt gcgtctaatt tcaaatgatc acaaacaatt tggtccatcc cttgtgcctg
     1081 caagttcagt ggaatttggt aaaggtgttc aacatccaac gattcgataa cggcttctgg
     1141 tgccacatca cagaactggg ccagtttatt tttaattcct tgaccagctg gctcttctgt
     1201 acgaataacc aacatatttg gttggattcc caagccacgc aattctttga cagagtgttg
     1261 ggttggtttg gttttcattt caccagcagc cttgaggtaa ggaagcaagg ttgtatggat
     1321 atacatgaca ttatccgcac ccacatctgc cttcatctga cgaagagcct ctaggaatgg
     1381 caaggactcg atatctccta ctgttccacc aacctctgtg ataatgacat cagagtcggt
     1441 cgttagagcg gcacgcttga ttttttcttt caaagcatct gtgatatgag gaatgacttg
     1501 aacagttgcc ccaaggtatt ctccacggcg ttctttacga agaacttcac tgtaaatttt
     1561 cccagttgtc acgttggaat atttgttgag attgatatcg atgaaacgtt catagtgacc
     1621 caagtccaaa tctgtctcag ctccgtcatc tgtcacaaaa acttccccgt gctggtaagg
     1681 actcatggtt cccggatcaa tattgatata agggtcaaac ttttgaatgg ttactttgag
     1741 accacgattt ttcaagagac ggcctagact cgctgccaca atccctttcc caatagacga
     1801 taccacacca ccagttacaa aaatatattt cgtagacata gattcctctt tctaaaatgc
     1861 tcaaggtctt gttaactacg aggggatata gaaaacaaga ccctactaat aagaataatc
     1921 tgggacgact gcaccagatt cacagctaat actcttcgaa aatctcttca aaccgcgtca
     1981 acgtcgcctt gccgtagata tgtgttcctg actttgtcag tcttatctac aacctcaaaa
     2041 tagtgttttg agcagcctac ggctagtttc ctagcttgct ctttgatttt cattgagtat
     2101 aaaatacaag aagataactt cttgaaaaaa caaaaatagc tccctaagaa ctagggagcc
     2161 ccgacctcta aaagaggtgc ccaaacaata tgatacctaa aaataggata attgtcaata
     2221 gctaactctt attcctcgct ggtttcagca tcgtcatctg aaaactcgtc gtcgtcttcg
     2281 ttgagatcca catcttctcc caagtcatct ggagcgattt cgttgatttc tgcatcataa
     2341 gcttccactt catttttttc atcatctgga ttttcatcat cgtatgaaag agctggatct
     2401 gcttcgtatg catcttcgtc ttctggatca tctgcattgt agtcaatggc atctgaatca
     2461 ccatccataa aggcattgac acgttttttc ttagcttttg gtgctacttc atcgtcgtca
     2521 ttttcttcaa gagcgatgat ttcttcgtcg atttcgtcca caccatacca tgaacgaaga
     2581 ccccatttgt tgtccccaag tgagatgaag ctaccgtcaa agttcaactc tgtgtagaac
     2641 aaaggcaaag cttcgcggat atcgctgttt gatgttccaa ggtagttttg aatttcgttt
     2701 acaagatcgc taaaatgcat ctcatgatcg cgaccacgaa gttccaagat agcacgcgct
     2761 acctcaatca tagatagttc acttttttct tgcccagcaa atacttctaa ttccaaagcg
     2821 tttctcctca tttatactac tatcgccaga gcgaacagac tctgacctca ttttatcatt
     2881 tactctttat tttacgataa ttttgcggaa tagtcaaagg ttaaggggga gaaagtggca
     2941 ggattagact aattccaata taaaactcct tcctttttct gttgctccat tttccacaaa
     3001 tccaagcgac ttgaaacacc tcctagaagc atgattgtag gtgtagattt ccttgactct
     3061 caattctttc catcctttta ctcgagccaa ttcaatcaaa gcacttagaa tcttttttcc
     3121 aagtcctcga tgttggtaag cggaattccc aatcacaatg gggagattat cctgagatag
     3181 tgtaatatcc ccaattggaa accattctcc cttctccttg acttcaatcc aaaaaagctc
     3241 accatgccga tccaaatagg aatacatggc ttccaaggtc gcttgactgt aaggaagctt
     3301 caccccatct acgaggtaaa ccaagttcac atcctgatac caagcaaaag cttcctcaca
     3361 ataattgacc ttataataag gaacaaaaca caatgcacta ccttttcaac aaaagagttg
     3421 ctgcttgatt aaaaccatca caccagttat accattttgc ttcataccca tcttgagcta
     3481 ggatacgatc ttctaaatca aaaacagagt aaatctttct ttcctcgcaa gcttgcgcat
     3541 agagatgata tagttcatca ccaccacctc tatcccactc agcagaaatc gtatcccgac
     3601 ctgccaataa agcctgataa gccctgtgat gcccatctgt aatcagcaaa caatctccaa
     3661 aggcaagaat actgattgga tcgacttgga ttgtttctgc cgactggtaa agcatctgaa
     3721 tatcttgcaa cttcttttct gataaatata gttgagtcgg atgaagatct gctatattga
     3781 ctttcatttc tttctctcaa gggaattcga tactcacttc tgtttgcctt taaatcgcca
     3841 ttggaagcgg agcttgtcat aaaagggaaa ctcgataaac aggactccca agcccacaca
     3901 gagactggca aggacgtctg atgggtaatg aactcccaga tagactcttg ataccagcac
     3961 actgactagg tagaggccaa ggacgatttg tacgattttt ctccagacct gatctttaat
     4021 ccgctgacta agaataacaa tcaaagtccc taccatcagc gttacagcta gagaatgccc
     4081 acttgggaag gaaaatccct tctcctccac cagatgtaaa atagctggtc gtgggcgctg
     4141 gtagatattt ttaaaggtca cgattaaaag acctgccaaa gccagatttc ccagcatgaa
     4201 gaaactttct atcttccatc gcttacgata aaagacaaaa gctataatga caacccaagt
     4261 gataatcact ggaatatcaa tcagacgtgt gagggatcga aaaagaatag tcaaataatc
     4321 tggtaagtct cctcgaatgg cagtctgaat cgattggtca aaaccgacca gcgtttcagg
     4381 gtaaaatttg accatgtagc caagaataac gaaaagtaaa agggcaaaac tgcccttcat
     4441 taaaaatgtt tgtttatctc tcataatgtt ttaaggttgg tttcaagaga acatacaaca
     4501 accagaatga aacggaaaag attacaccct caatcaagtt aaaaggtaat accatggtca
     4561 ttaggtagtt ggaaagtccc aaaatttttc caatatcaaa gttagcaaac ttagcgtaca
     4621 aaggaacagc ataaacatag ttgagaacca acatggccaa ggttaaacca acagttccag
     4681 ctagagagcc tagtaggaaa cgaagggttg tccgttcctt tttccaaatc aaagcaaata
     4741 cgatgacaaa aactcccaaa gctacgatat tcatcggcaa accaatgtaa gtattcactc
     4801 cttggctgtt aagaagcaat ttcaatagtg agcgaagcaa gagcactcct agagcagcag
     4861 gcaaatccat gaccaccaga ccaacaagga ctggcaagat actaaattcg atcttgagga
     4921 aagatgccgc tggtaaaagc ggaaagtcaa agtacatcag cacaaatgag atggctgata
     4981 gaattgcaat ggtcgaaagt cgacgtgtgt ttgtcataac aggttcctcc aattttctat
     5041 aaaatcagaa gaagttggaa aggattcctc tatctattct cactttttat atcccaaaag
     5101 ttccctctta ctctattaaa gaaaaacaaa gcaagtggtt acaatccggc tataaatcta
     5161 tcaaaacaga caaagctatt ctttcgtctt ctcccatcca gactatactg tcggttgtgg
     5221 aatctcacca catcagcttg cgctcgcgga cttctttaaa gagaaggaaa tagagatttt
     5281 tcttttagac taggcgacaa gctgtaggca taacttctgg ttaggcaaag cttgtcaaca
     5341 aagtataaaa ggaaaagatc atttcctagt taaagtcacc gccggtcggg aatcacaccc
     5401 tgccctgaag actcttttat cataacaaaa aacgcttgca agcgcaagca ttttgttcat
     5461 tttcattttt tatttcaatt tatccacttc ataggtatgg acgagttcaa gacctgcaaa
     5521 gttctgctgg cgaagagcct catagacaat catgcagacg gtattagaca cattgagact
     5581 gcggacatgt tcatcattca tgggaatacg gagagccttc tcaggatgtt ctcgcataaa
     5641 atcttcaggt aagcccttgt cttcacgtcc aaagagaaaa taatggtcct cgtcagtcga
     5701 taaatccacc tcagaataga ttttttccgc gaatttcgaa atcagataga gttttccctt
     5761 catctgagat atgaaatctt ctaaactctc gtaaaaataa atctctagct tatcccagta
     5821 atccaatcca gcccgcttca tcttgcggtc atcaatagga aagcccatag gcttgatgat
     5881 gtggagggga gaattggtcg cagcgcaagt acgcgcgata ttgcctgtat tttgtggaat
     5941 ttgaggttca aataatacaa tgtgatttgt catgacttgc ttcctttcac cattgcaaaa
     6001 aaatagccac actgcccgga gtcaagctca gcaaacagcg tggttaaggc atcgttaact
     6061 tacctcacaa caggtttgaa gtaaatcagc gaaactactt tcttagtata acactttcag
     6121 aatcattgtc aatagaaatg acttgatttt ttcaattttt tcaagctatt tccaagggtt
     6181 gtaaaatcgt ccctgattct gcaagataag tagtaaacta actactaaaa acaaggttgc
     6241 caagagcaag gtaatatagt ctcctttttt caaggcctga taactatacc atgtgcgttt
     6301 tttctctttc ccaaagcggc gaagctccat ggcagtcgca atggtatcaa tgcgttctag
     6361 cgagctaaaa atcaagggcg taataatgag cagattgcct ttgattcgtt gcataagaga
     6421 agctttcttg gataattcca tcccacgcgc ctcctgagac atcttgatag taaagaattc
     6481 ttcctgcaaa tctggaatat agcgcaaggt caggctgaca gaataagcaa tcttataggg
     6541 cacaccaatt tgatttaaac tggaagcaaa ctgactagga tgggttgtca tcaaaaagat
     6601 aatagccaga ggaatggtgc aaagatactt aatggccaaa tttagcagat aaaagagctc
     6661 ctggctggtt agagtgtaga caccgattcc ctgccaaatc acacttctct ctccataaag
     6721 tccaacccca tactcgggag aaaagagata gaccatcaaa acgtttaaaa cggcaaatac
     6781 cgtcgcaaaa acggctacaa aggaaacatc tttaaagcga atttctgata aatagaggag
     6841 aaagactgaa aagatggcaa tcagcaagag cattctggta tcatagctaa tcatggccgc
     6901 caatgatacc agaatgaaaa agagaagttt cccagctcct gacaagcgat gaatcacagt
     6961 atctctatgc tggtaaccga ttaatttagc ttgcatccct ctctcctttc tttgtaaaat
     7021 gccgttaaat ccagtggatc cacatctagt ttcttagcca agttaaagat ggaggtttct
     7081 tttagattgg cttttactaa cagctcagga tcgctcaaca gactggctgg aacagtatcg
     7141 gcaatcaatt ctccatccac catgacaagg acccggtctg aataatccag catcaattgc
     7201 atatcatggg taatcatgac aatggtatgc cctttttgat gtaactcttc gagaaattcc
     7261 ataatctcag tatagttctt ctgatcttga cctgcagtcg gttcatctag gagaataatt
     7321 tcagctccta agaccaaaat tgaagcaatg gtgacacgtt ttttctgacc aaatgacagg
     7381 gcagaaatag gccaattacg gaattcataa agtccacaga ttttcaaggt ttcatatact
     7441 ctcgtttcaa tttccttctc atccacacct cgcaaacgga gccctagagc cacctcatca
     7501 aaaatcatat tggttgaaat catttgatta ggattttgta gcacatatcc tactcgttcc
     7561 gcccgctctg caacagaatc gccttttata tcctgttttt cccaaagata gcgtccttcc
     7621 gtctgaataa agctacttat agccttggct agagttgatt tccctgctcc attttttccg
     7681 acaatagcaa tcttttcacc ctttttaata tctaaatgta gggattttaa aatcggtcta
     7741 tcatcataag aaaaagatac ttcctctagt ctaaagagtg actgcaatgc tggggtttct
     7801 tttgccagtt cattctgcaa ctgaacctga ccttttgaga tagacaagtt atccagattc
     7861 gctaattgtt cttccttgac taagtccaca cctaattgac ggagagtcgt tagataaagg
     7921 ggttctcgaa ttccattttg agtcaataaa tcagtcgcaa gcaactggtc agggctccca
     7981 ttaaaaagga tacgaccatc gtttatcaag acaatccgat ccacagggcg atgcagaacg
     8041 tcctccaaac ggtgctcgat aataagagtc gtcgtcccct cttccttatg aatctggtca
     8101 atcaattcga taatatcctg acctgacttg ggatctagat tggcgagtgg ctcatcaaac
     8161 aagagaatcg gactttcatc aatcaagaca ccagccagac tgactcgctg cttttgtcca
     8221 cctgacaaat cctgaggacg ctgatccagt aaaggaagaa ggtccagctt ttcagcccat
     8281 ttataaacac gacctttcat ctcatctagg gctgtcacat cattttccag agcaaacgcc
     8341 aaatcttctg ccacagacaa gccaataaac tgcccatctg tatcctgcaa aactgtgcta
     8401 accagatgag acttatcata gatgctcata tcaaaggctg cttgaccctt tatcaaaaat
     8461 tctccatatg tctgaccctt gtaaatattg ggaataatcc cattcaaaca ctgacccaat
     8521 gtagatttac ctgatccaga tggtccaaca attaagactt tctctccctt gtaaatggtc
     8581 aagtctatcc cttgcaaggt cggttcttgt tgtgtttcat accggaaaga gaaatccttc
     8641 cactcaatta tagcttcttt catcttactc tcttcattcg cttcttagac ttctatttta
     8701 tcataaatca agcccttctt gcagtctctt ctcttaaaat cttagcgcca aaaagattcc
     8761 tatcttagct tacttgccta actaatctat aaacatcgaa aaagactagt tgcccagcct
     8821 tccccatcat tttatactct tcgaaaatct cttcaaacca cgtcagtttc gccttgccgt
     8881 a
//
LOCUS       DAVAAL010000073         8779 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVAAL010000073 DAVAAL010000000
VERSION     DAVAAL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8779
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.074"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(104..1528)
                     /locus_tag="VQM60_001388"
     CDS             complement(104..1528)
                     /locus_tag="VQM60_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET1081252.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2072..4351)
                     /gene="pulA"
                     /locus_tag="VQM60_001389"
     CDS             complement(2072..4351)
                     /gene="pulA"
                     /locus_tag="VQM60_001389"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET1081253.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHFLVVVNAKSDPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4463..6421)
                     /gene="ligA"
                     /locus_tag="VQM60_001390"
     CDS             complement(4463..6421)
                     /gene="ligA"
                     /locus_tag="VQM60_001390"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET1081254.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6514..7692)
                     /locus_tag="VQM60_001391"
     CDS             complement(6514..7692)
                     /locus_tag="VQM60_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET1081255.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7772..8635)
                     /locus_tag="VQM60_001392"
     CDS             complement(7772..8635)
                     /locus_tag="VQM60_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET1081256.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLDHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8638..>8779)
                     /locus_tag="VQM60_001393"
     CDS             complement(8638..>8779)
                     /locus_tag="VQM60_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081257.1"
                     /translation="QSQAISEQMLETNDADKLMELQAELDKISHRQEEAMLEWEELSD
                     QV"
BASE COUNT         2674 a         1931 c         1559 g         2615 t
ORIGIN      
        1 gtttcaatct attataatac caaaaagttc gtattttttc tataattatt attattttat
       61 agcaaaaaat aaaaccagga aaacaatttc ctgattttac acgttatttc acatcaaaaa
      121 caatggattt gacatttgtc atcgcttcaa tgctatattt aattccttgc actccagctc
      181 cagaaccttt gacaccaagg aatgggaaat tatctggacc acgctgggtt ttattattaa
      241 tgtggactgt acctacttca agtttttcag caatttcaaa ggcttttttg aaatcatttg
      301 taaaaactga tgattgaagg ccgaattcag attcgttggc aaaggcaata gcctcctcta
      361 cactagccac acgaatgatt ggtaaaacag gaccaaatgg ctcttcccat gccactttca
      421 tatcttttgt aacttggtca aaaagcactg gccagaggag attgccctca cgtttgattg
      481 gtgtaagagc ctgagcctct ttttcttgtg catcctcaat caagccccaa atgaagtcgg
      541 ctgaagcatt gtcaataaca ggtgtaatat cagcattgtc aaatggatca ccaactgtta
      601 atttagaaac ttcttcctga agcaaagtag ctaatttatc tgctacactt tcgagaacaa
      661 tgacacgttt aatggccgtg caacgttgtc ctgagtagct aaaggctccc gcaacaattt
      721 gcttggcagc atgttccaaa tctgcatctt ctagtacaag agctgcatct ttcccaccaa
      781 gttccaacat gataggacgc ataccagcta aacgaccaat acgttctcca ataggagttg
      841 aacctgtaaa gttgatgaag ttgacttctt tgtgctcaat gatataatcc ccaatttctg
      901 aaccacgacc tgtaatggtg ttgaaaacac ctgccggaat ccctgcttct tcaaatgctt
      961 tagccaacaa gagtccagaa atggaacctt gtgttggtgg cttaaacatg accacattcc
     1021 ctgcaatcaa ggcaggtgca attttagaag cagataaatt aactggataa ttaaaaggag
     1081 caatcgctag cacgatacca actggttcac gacggacaac agccagtttg tttttacttg
     1141 ctgcctcaaa accaccacct tccattgctt gtccagtgat acggagacct tcctcagcag
     1201 cataacgaat caagtctgct gtacgcacta cttctccaat tgctgcttta atcccttttg
     1261 ctacttcttt ggcaaggata gtaccaattt tttccttatc gcgttctaaa atagctgctg
     1321 ttttatgcaa ataagccgca cgttcaattg ctgataaagc tcgccatgct ggcagggctg
     1381 cacgcgcagc ttgcatagcc tcatcagctt cagtctgagt catggctgga actgtaccca
     1441 attcttcttg attgattggt gagtaaatcg taatttcttg ttcagatgat ttccattttc
     1501 catttactaa attctgatat cttgtcaaat tcttgtcctc ctgaaaatga ttaagatata
     1561 ttttaccaaa ttttcagaaa atcacaaccc tttttgcaaa atttttgaga ttattttcac
     1621 aaacttgatt tttcaaagta tactcaataa aaataaaaaa aatccactac gtcaaggcga
     1681 ggctaatgtg gtttgaagaa attttcgaag agcgtgaatg agtatcatct atagtaaaat
     1741 aaaaaactga acaatttggt tggggacagc caaaccaatt tctcacaatg tttcagaaac
     1801 aagggtgtgc tactccaatt tcggcctact ataactgtca tagattgctg aaacaaagtc
     1861 taggtaaaag tcttcataat aaaaagacct cctatcaaat gttcaaaaac tttgatagga
     1921 ggtcttgttt cgtgaaaata tttatcaaat tttctataca agtgagctgt tagccaggtt
     1981 ctttctattc tttcaatttc aatgaatgga ttttttacca atactcataa ctgggaattt
     2041 ttctgtctaa aaatagcgag atagatggta tttataaaac actcaagaca gctagactaa
     2101 tatcatttaa aacattatct tcttttgagc gactgttggt taccaacata gctaaatttc
     2161 ctgcattttc aaattgatag gggtctgatt tagcattcac aaccaccaag aagtgttctt
     2221 tgccgtgaac ttcatagata aggtagccgc tatgttcaat cgcagaatgc acaaagacat
     2281 gatggtaaat ttcatcatag ctagagtaag aaaaggcacc agtttttgtc ttcaatcgga
     2341 tgacttgacg gataaactca atactgtctt gacgctcatt aatcaagttc cagttcactt
     2401 ggttcacact gtcaggagca ttatagctat tcatcgcacg ctctctatca tcatgggtta
     2461 actcaccatt ttcaccagtc gcaaccagtt tggtacgacc aaattcttga ccgatttcca
     2521 taaaggccat cccctgcatg agcagattca tggctgtggc agtttcgacc ttgcgcatga
     2581 tttgctctga actttggtct ggatgaaggg ttgccaataa atcgtgaaga ttgtaattgt
     2641 catgggcttc tacatagtta agcacctgat ttggatgtgt atagcttcct aattcacgac
     2701 ttcctaggat tgctttagct agaattggct ctgtcgcagc accactgaca aaacctgact
     2761 tgatagcacc ataaacttct ccccctttga cagcatcgcg ctgattgtca ttaaagaaac
     2821 caatatttgg catctggtag gcattgtcct tcttggcctt atcataaggg gcaagacctg
     2881 ttcccatatc ccatccttct ccatagagga taatgttgga gtcgatttca tccaagcttt
     2941 gacgaatcat ctgcatggtc ttgacatcat gaatccccat caagtcaaaa cggaagccgt
     3001 caatattata ttcctgcacc cagtatagaa gagaatcaat catatacttg cgaaacattt
     3061 cgtgttcact ggctgtttca tttccaacac ccgttccatt ctggaaggta ccatctggat
     3121 tcatacgata atagtaatca gggactgttg tttggaatgg tgcatcaaca actgagaagg
     3181 tatggttata gactacatcc ataatgactc caatacccgc atcgtgataa gcttgaacca
     3241 tcaccttcaa atcacgaatg acctgagctg gatcatctgg attagttgaa aaactagttt
     3301 ctggcgcgtt atagttttgt ggatcataac cccagttgta ggttacattt ccatcctcat
     3361 cgtattcttt atgacggtct gcaattggtt gcaattgaac ataattgtag cccagcttct
     3421 tgatgtaatc aaaagcagtt gactggccgt attggttaac tgttccagcc tgagcagcac
     3481 ccaagaaagt tcctcgaaga tgttcatcta cacccgatgt aggtgattta gtcaaatcac
     3541 gaatgtgcat ttcacagata actgccttac atggattttc caagcgccaa gtagcctccg
     3601 aaccgtgctt aacctcgaag ttttcaactt gcttttctac atggctcaga atagctgaac
     3661 gtttgccatc agggctggtc gcgattgtat aaggatcacg tgtcagtgtt tggtgatgag
     3721 ggaattggac ttgatactga taagtcttac ctaccaaatc ttcttcaaca tccaaactcc
     3781 agacaccgat tgtattgtcc ttatgattat aagagtagct attgcctctt ttcatctcaa
     3841 aagtcttcca aacgggtgca tcattagcag ctgattcata aacgacaact tgcacttctg
     3901 tcgctgtagg tgaccagaga gaaaaatgag cctgattgtc ctctacacgg caacccaatt
     3961 ctccttggta accccaatga tgatcaaaac tagcactgtt aatggcctta tcaaaggcaa
     4021 aaggattttg atttttatag aaaggactgg caatagcagg attttcagag taataaatcc
     4081 tatcatcgcc ttccaaaatc cagacctctg ttaatagggg atagtgatta aaacggatag
     4141 aatattcttt actagtttga cctgtatgaa ccacaaaatt caagctttct ataacatgtg
     4201 aacttgggtg ttcaaagcta aataaagctc caaaataatc ttctttgtag gttagcaaat
     4261 caattcgttg atcctgactt tttacaaagg agcaagtgtc atattctcca ttcttacgat
     4321 ggtaatgaat gcgcataggg tagttataca ttttttattt ttccttttta ctttgtttct
     4381 atttcactaa taaatttttg tcaatctcgt ctcaattaac agacatagtc atattctcta
     4441 aactctgttt ttaaacgatc cattacaaac tttctagcca tgcctcatct ctgacctgga
     4501 taccaagttc ttgtgctttt tgcagtttac ttccagcgtc tgcacctacc acgacgaggt
     4561 cggtcttttt agaaacacta cctgtcactt tggcacccag actttcgagt ttacttttag
     4621 cttctgagcg cttgagtcgt tccaattttc ctgtcaatac cacggtcaaa cctgacaagg
     4681 ccgcatccgc tactaccgtc tgtcctttat agtccagatt gaccccagtt tctttcaatt
     4741 ctctgagcag aatttcagag ccttctgtcg caaaataagt ctgaagactt ttggcaatca
     4801 cgccacctag actttcaata ctagccactt cctctgaatc tgcctgatac agattttcaa
     4861 ttgaatggaa atattgaagt aaaagctgac tagccttgct tccgacatga cgaattccca
     4921 aaccaaataa gagcttctcg gcagaatttt cctttgatgc ttggatagcc tgatacagtt
     4981 tagcagcgga cttttcctta accccctcta aaaggaggaa atcctcttct tgcaaacgat
     5041 aaatatccgc cacatccttg actaaattag cagcaaaaag cttctcaaca atagatggac
     5101 caaggcctgt aatattcata gcatcacgag aagcaaagtg aatcaagcct tccatgattt
     5161 gagcagggca acgcggattg atacaacgta gggccacttc atcttcaaag tgcaacaagt
     5221 cagagttaca acttggacag tttgtaggga tatctagttt ttcttcagaa acccgtttgg
     5281 actctaccac acgtaaaacg gcagggatga tgtcaccagc cttatataca atgaccgtat
     5341 cgtcttttcg gatatctttt tcagcaatat aatctacatt gtgcagggtc gcacggctaa
     5401 cagtcgtacc ggcaagttgt actggtgtta gattagcagt tggagttaca acaccggtac
     5461 ggccaactgt ccagtcaact gataagagtt gagcttcttt ttcttcagca gggaacttgt
     5521 aggctactgc ccactttgga gccttaacgg taaaaccaag ttcttcttga cttgctaggt
     5581 cgttgacctt gattaccact ccatcaatat cgtaaggcag attttcccgt tcctgtccta
     5641 cttcttggat aaaattccag atttcatcta tgttttcagc caagattcgc ttaggattga
     5701 ccacaaaacc tagttgttct aggtacttca aacccttttc ttggctatca cgagttgaag
     5761 ggctggcttc ttgatagaga aacgttgcaa gattacgctt ggcaactact gctgtatcca
     5821 actgacgcag agttcctgct gccgcattac gaggattagc aaattcaggc tctccatttt
     5881 cttggcgcac ttggttaact tggtcaaagg aagcgcgtgg catgtaacat tccccacgaa
     5941 ctgtgatatc tagttcttct ggcaaagtca aagggatgtc cttaacacgc ttgaggtttt
     6001 ctgtgatatt ttcaccaatt gaaccatctc cacgtgttac cccagcaacc aaaatcccct
     6061 tttcataagt cagcgagata gataagccat cgattttcag ctcacaaata taggtcggat
     6121 gagccacttc cttacgaaca cgcgcatcaa aagcatctag ctcctcacgt gaaaaagcat
     6181 cctgcaaact ataaagagga tactgatgac tgtatttttc aaaaccatct aaaaccttgc
     6241 caccaacacg atgagtcgga ctgtctgcta gcacttgctc tggataagca gtttctaact
     6301 cgaccaactc acggtaaagg cggtcatact cactgtctga aaccgaggga ttatcgctgg
     6361 tatagtactc agtcgcatag cgattgagca aagcgactaa ctcattcatt cttttattca
     6421 taagaccatt ttaccataaa acaagccctc ctcacaaacg agaaggacgg aaaaaacact
     6481 tagtttgaaa ttatttttga aactcaagca accttatatc aatttttcaa aatgagttcg
     6541 aacataaata aacgatatac aagacaagat gataacacca cttccaatta tcaggaaaga
     6601 agagagatgt acacttggca agactgtcat aaatcctttt gcaataggca taaatagaat
     6661 agctaaggta aaaattgtac tcagtactct cccaagaaat tcgctctcaa ccttggtttg
     6721 tacttgagta aaaaagtgaa tattaaaaat cgtcataaac aattcacaaa ctaaatttcc
     6781 agaaaaggaa agaaaagttg gaagtggtaa tcccatcata aaaactccga cacctgtcaa
     6841 agccagtaaa atcaaaagat tataaatatt agctttaatt ttactagcta gaagagcccc
     6901 aatgatggaa ccaatagccc ccatagttaa aatacttgca taggctcctt ctgacccgta
     6961 aagctgattc gaaaagggaa gtagaaattc aaaagctgca aaaaagaaat taacgctgga
     7021 agctaccagc aaaaggaaga aaatttcttg ctgatgccag atatagtgta acccatcctt
     7081 gatatctaca aaaatatctc tcccagtaaa agcctttttc tcttgaactt ttgcttcctc
     7141 ttttggaagg aaagccacta gaacaaaagc aatgaaaaaa gtcagcgagt ctagcagtag
     7201 cgtcatatgg agacttgcaa actgtaaaac aaggaaggaa agaacaggag agctaacacc
     7261 tacaacctgc aaaaccagct ctaggcgaga attatagatc acaatctcat ctttctccac
     7321 cacttcagtt atgatagctt tattggctgt gcgagaaaag gcaaaagcaa tagcctgcac
     7381 aatgttagca acaatcaaag cgccaatcat ccagctatca ttccttatga aagaaatagc
     7441 cagacaaaga atcccacaaa caagatctgc cgtcattaaa atcttacgac gagaaaaacg
     7501 gtctgaaata actccgccaa agggattgac gagaatagat gtgacgagct cagaaatctg
     7561 atacattcct aaaactgtct gtcctatagt ccccatggaa gccaaccaga cactatttcc
     7621 ataatcatag agcatatttc ccattttatt gatagcccca cggctaatca actgcactgc
     7681 atagcgattc atattaaagc tcctctcaaa ttttgaaact attgtatcaa aaccgaaagg
     7741 agctttttat tttttccctt atttgggaaa attaactttt gacaaatttt tcgtagtgtt
     7801 cctgataata ggctacttgc tctggaagac ctaacacatc aaaaatatgc atggcctctt
     7861 gcatctgctt acagccttct ttacactgtc ctttttgata taaggcaaaa ccttttaaat
     7921 aatggaaaac attacgctca taaagcttaa tacctttgtc aataatcttc tctgtataag
     7981 cctcaaaata gttggcatta taaaaagaag aatgatctaa acaatgctgg taacaattga
     8041 gggccaaaat caacactaat ctcttatggc gactaatctc ttggtaaaat tcctccctct
     8101 ccataacttc tctaccaatc cgagtgacat agtctacatc gtagaaacta tagaggttac
     8161 cgaaaagaat caactcatac atggtccatt cttctgtttt gaagagataa tctgctacct
     8221 tatccaaatc atcctgcttc atatcataac tcgcatctct ttgacaaatc agaccttgta
     8281 gcaaaatcca gttcagctca aaataaaggg gagtcgtcga actcttagac ttttcaagtt
     8341 gttctctttg aagcttttga aaacctgcaa tatcgtttga atagtaaagt gggataatct
     8401 gtgccatcat agacacatgt tcatgattat gaaaattcct tgccttatcc atgaaatttt
     8461 cgattgttac atgaatgtta tccaaaatct caaagaaacg ggagactgcc aggtcagact
     8521 ccccaagctc aaagcgagat aactgagagg tagagcagga ttcgcctgct gcttccttta
     8581 aagaataatt tccacttgtt cgaaattcac gaaatacttt tccaagatgt tccatcttta
     8641 cacctgatct gataattctt cccactcaag catagcttct tcctgacgat ggctgatttt
     8701 gtccagctca gcctgtaatt ccatgagttt gtcggcatcg tttgtttcca acatttgttc
     8761 agaaatggct tggctttga
//
LOCUS       DAVAAL010000074         8676 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVAAL010000074 DAVAAL010000000
VERSION     DAVAAL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.075"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            361..1194
                     /locus_tag="VQM60_001394"
     CDS             361..1194
                     /locus_tag="VQM60_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET1081258.1"
                     /translation="MNPLKECYEKNGILIAAHRGTNGGNILQNTSKAYLNSIRHNADM
                     FEVDVIRSKDGEFFAFHDGQEPLVFGEKIDIRTLSSEEIQRKECFNSLNEPILQKIEK
                     ISDVLKDFEGQSLINIDRSWFYWDTFLDKLKIYPSLNQVLLKSPVKKELLEMLRDSGL
                     NVMYMPIIKTIEELDFVLSFEGINTVAVELVFQDLENPIISKKIIDDLHEKGLLIWVN
                     ALTLSDSIILSAKIDDDTAIAHDGESWGKLVSIGFDIIQTDWPLLLYQYLVEKNKKIK
                     K"
     gene            1229..2290
                     /locus_tag="VQM60_001395"
     CDS             1229..2290
                     /locus_tag="VQM60_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET1081259.1"
                     /translation="MFKNFKKIAFTSSLILLAACSNNASNTNQSTDEGSVDKSQEIVI
                     YTNSASNGRADWLQTKASEQGYNLRLVDISGGELADRLIAEKNNAVADMVIGLNKLEF
                     NRIKAEKLLVKYSPKWADEVDSSLGDSEGYYSPIVNQPLVLIGNKDAKMPSDWTDFTD
                     SSYKGKYGISKLSTGTSKNIFASIVSRYKDEKGELGISDEGWKVAKAYLKNAHVYAEG
                     EDYISSIMDNNNDLKYSMMWGSGVLQNQKEREYKFQVMSPKVGVPYVTEQVGILNTSK
                     KQALLKEFVDWFGSSELQKEWSDKFGSIPANKKALEQAKDELKQFANSVKPQELDWEF
                     IGKNIDSWIEKAQLEFVEQ"
     gene            2307..3311
                     /locus_tag="VQM60_001396"
     CDS             2307..3311
                     /locus_tag="VQM60_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081260.1"
                     /translation="MITFKDIQIKYNDFIAIDNLNLTINEGEFFTFLGPSGCGKSTTL
                     RALVGFLEPSQGQILVDNQDITNLDPEKREIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VSPEIIKAKVHEIAKKINISDEQLNRNISELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVSLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGFIEQVGTPY
                     DIYNQSATQFVCDFIGSINKISQEVLKKLQGENKYFKETEGYIRLERVKLEKTSPQDL
                     SITGVISDIEFNGVLIKYKVDLSNSESIHIIEKNDGLSRHIIKEKVTMYINPRDIMQF
                     "
     gene            3323..5017
                     /locus_tag="VQM60_001397"
     CDS             3323..5017
                     /locus_tag="VQM60_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET1081261.1"
                     /translation="MRKSLNWKDCLIRLGVLWLIFALIIYPNIELLSGVFYKNGSFTL
                     EAFNKIIHSQRAMSSIFNSFILAISMVFTVNIVGTLIVLLTEYWDIRGSKILKLGYMS
                     SLVYGGIVLATGYKFVYGANGLVTKTLSSIIPGLAPNWFQGFGAVLFIMTFACTSNHI
                     MFLTNAVRSVDYHTIEAAKNMGASPLKVFFSVVFPTFKPTLFALTILTFLTGLSAVSA
                     PLIVGGKDFQTINPLIISFAKTTSSRDLAALLAIMLGLATILLLTLLNHVEKGGNYVS
                     VSKTKASIKKQIISSPLWNTLAHIVAYVLFAIYMLPIVLIILYSFTDSLTIKTGTLDL
                     SKFTLANYANLFTDAQSFRPYLVSVVYAILAAVIATIIAIVISRIVHKNKSKFDKFFE
                     YGALIPWILPGTLIALGLMFTYNIPHLILFNLVLVGTVIILLIAYTIQKLPFSYRMIR
                     AVFFSIDNDMEEAARSMGASSFYTMVRVIIPYILPVVLSVVVLNFNSLLSDYDLSVFL
                     YHPLFQPLGIVIKQSTDETATLNAQAMMFVYSVILMIMSSAALYLSSLFQGKRGKR"
     gene            5019..5720
                     /locus_tag="VQM60_001398"
     CDS             5019..5720
                     /locus_tag="VQM60_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET1081262.1"
                     /translation="MNLSYELSIWEILLRCFLAILFGGIVGYERGVKNQAAGIRTYLL
                     VCLGTCVVMMTNQFITSRYGSGDPSRMGAQVISGLGFLGAGTIMVTNQKVKGLTTAAG
                     LWSSATIGLAIGIGFYEGAILAGVVLIAVLTLFTPLKDYLNSKTKIADYYLVFSSLEG
                     YNRLLIYLNTSGIKILEIQNGIGNLDEERQLNKIKSTKVSCYLSLKLNENFEHLKLME
                     EMHNIAGVIYVEEIR"
     gene            5767..6366
                     /locus_tag="VQM60_001399"
     CDS             5767..6366
                     /locus_tag="VQM60_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET1081263.1"
                     /translation="MNIIFDLGNVLIEWNKEKILSKICKNDLEYNLFNKFVFQSNLWI
                     DLDNGKISLEFLENQLIDEMGHQYQDQIHELVWNWFNYVDLYDEVYELIKQLKKKNFQ
                     IYVLSNTSSIFHILLDSVLSKVSSVLDGYVISCEVKMMKPQKEIYLSLVNKYQLDIKD
                     CIFLDDLEENVEAARTLGIKAFQIKERKEISNILKDLLN"
     gene            6376..8403
                     /locus_tag="VQM60_001400"
     CDS             6376..8403
                     /locus_tag="VQM60_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET1081264.1"
                     /translation="MVTIKDIARVSGVSHGTVSNVLNKKGNVSAAKIKLVEETAKKLG
                     YNMNSQAQFLRKGASSKCFFLLFNNARKKYAEYFAEIDQLSLDVEYVYLHKFSEIKNK
                     ISAILSENPQFIVCLGFSPKRFVDIEDNIPIFEVDTYQSNKTLSSFDSDKIFEIVLEL
                     VRTEKLSNLYFISLTNEKEHSLFEYLTQKIKNSTHIQVQERLQLLSLYPTLKNFAKYD
                     CIFVDDEDLMHTLLDLFDWFTIIDRPKICLIGSRVWFEKRNLFYIHLDYRILALNTAE
                     NLESGNSSIVPVKDGKQILTNQLFDGECDDIYLLTVHSPMNKALEILSEKYKQISGKN
                     IKIIEKRYDELLEMIESGDVSSSFDMIRMDMAWLPTFGKKYFQEITSFRQTKSINQNI
                     SKSVSREYMYIDQKQYTFPLDVSSQILVYRKDLFEDSFLQRRYFEKTKRKLTVPKSYQ
                     EFDELSEFFTLTENPFSPTLYGHSLALSSSVRAACEFIPRFRERLAQSRMNLAVLPQV
                     LTEYEKSYQFTEKSFNSSWNDFVTNLNSGKTSMEIIFSNYTSPLFDGLNVSAQFEFAT
                     ATIPGNTPVIGGGSIGISKYSNRVEECLNFINWLYSEEISILLTSLGGFLPSKYVMQN
                     RMLQFQYPWLSSLEDTFSIGSRTKWYGFNTDFRFEQMLGQELIESVKHRYG"
     gene            8562..>8676
                     /locus_tag="VQM60_001401"
     CDS             8562..>8676
                     /locus_tag="VQM60_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET1081265.1"
                     /translation="MNYEASKQLTDTRFKRLVGVQRTTFEEILAVLKTAYQK"
BASE COUNT         3078 a         1114 c         1506 g         2978 t
ORIGIN      
        1 aactgaaata agatataaac aaataaattg gagcttaaca tccattttaa gctaattttt
       61 tagaaactac agtgtactac tctggattca acacattata attgaaattg aaggttagat
      121 ttaaagtaat aaagaataat attatgctct aaaattagat aaatttcaaa taattaagga
      181 tacctatcac tattataaag taactgaata acattttata tttaataatt atttaaaata
      241 ataaaattat aacattatag aaattatttt aaaaaataat gaaacgttcc aacttatcta
      301 ttgacacgtt ccaagaaatc ggatacaatg taattaacaa taaagatagg agaaaataat
      361 ttgaatccat taaaagaatg ctatgaaaaa aacggtatac taattgcagc acaccgtggt
      421 acaaacggag gaaatatatt acaaaacaca tcaaaagcct atttgaattc catacgccat
      481 aacgcagata tgtttgaagt tgatgtaatt cgttcaaagg atggagaatt ttttgcattt
      541 cacgatgggc aagaaccttt agtatttggt gaaaaaattg atattagaac tttgagttct
      601 gaagagatcc aaagaaagga atgttttaat tcactgaatg aacccatatt acaaaagatt
      661 gaaaaaattt ctgatgtatt aaaagatttt gagggacagt ctttaataaa tatagataga
      721 agttggtttt actgggatac cttcctagat aagttaaaaa tatatccatc tttgaatcaa
      781 gttcttttaa aatcacctgt aaaaaaagaa ttattggaaa tgcttagaga ctctggtttg
      841 aatgttatgt atatgccaat tatcaaaaca attgaagagt tagattttgt tctctcattt
      901 gaaggaataa atacagtagc tgtcgaactt gtattccaag atttagaaaa tccaatcata
      961 agtaagaaaa taattgatga tttacatgaa aaaggattac ttatttgggt taatgcttta
     1021 accttaagtg attcaattat tctttcggct aagattgatg atgatacagc tattgctcat
     1081 gatggcgaat cttgggggaa attggtttca ataggatttg atattattca aacagattgg
     1141 ccattattac tttatcaata tctagttgaa aaaaataaaa aaataaaaaa ataaaaaaat
     1201 aaaaaaataa aaataaagga gaactatcat gttcaaaaac tttaaaaaga ttgcattcac
     1261 ttcttcacta attttacttg ctgcttgctc aaacaatgct tcaaatacaa atcagtctac
     1321 tgatgaagga agtgttgata aatctcaaga aattgtaatc tatacaaatt cggcttctaa
     1381 tggacgtgca gattggttac aaactaaagc atcagagcaa ggatacaatc ttcgtttggt
     1441 agatatttca ggtggagaac tagctgaccg tttgattgca gaaaagaata atgctgtggc
     1501 tgatatggta attggattaa ataaattaga atttaatcgt attaaagctg aaaaacttct
     1561 tgtaaaatac tcaccaaaat gggctgatga agtagatagc tctttgggag attctgaagg
     1621 gtattattca ccaattgtaa atcaaccatt agttttgatt ggtaataaag atgctaaaat
     1681 gccatccgac tggacagatt ttactgattc atcttataaa ggtaaatatg gtatttcaaa
     1741 attaagtaca ggtacatcta agaacatctt tgcaagtatt gtttcgagat ataaagatga
     1801 aaaaggtgag ttaggaatat ctgatgaagg ttggaaagtt gcaaaagctt accttaaaaa
     1861 tgcacacgtt tatgcagaag gtgaagacta tatctcttca attatggata ataataatga
     1921 tctaaaatat agtatgatgt ggggatctgg tgttctacaa aatcaaaaag aacgtgaata
     1981 taaattccaa gtaatgtcac caaaagttgg tgttccttat gtaactgaac aagttgggat
     2041 tttaaataca agtaagaagc aagcattatt aaaagaattt gtagattggt ttggctcttc
     2101 agaacttcaa aaagaatggt ctgataaatt tggttcaatt ccagctaata aaaaagctct
     2161 agagcaagca aaagatgaac tcaaacagtt tgctaatagt gttaaaccac aagaacttga
     2221 ctgggagttt atcgggaaaa acatcgattc ttggattgaa aaagcacagc ttgaatttgt
     2281 tgaacaataa tagttaggat taagtgatga ttacatttaa ggatattcaa attaagtata
     2341 atgattttat tgccatcgat aatctcaatt taacaatcaa tgagggagag tttttcacat
     2401 ttttaggacc aagtggttgt gggaaatcaa caactcttcg tgcactagtt ggatttttgg
     2461 agccttcaca ggggcaaata ctagttgata atcaagatat tacgaattta gatccggaaa
     2521 aacgcgaaat tggtattgtg tttcaaagtt atgcattatt cccaacaatg actgtttttg
     2581 ataatattgc ttttggtcta aaagttaaaa aagtttctcc tgaaattatt aaagctaaag
     2641 ttcatgaaat tgcgaaaaaa attaatatct cagacgagca actaaaccga aatatttctg
     2701 aattatcagg gggacaacaa cagcgtgtag ctttagcacg tgcattagta ttagaaccaa
     2761 aaattctttg tttggatgaa ccgttatcaa acctagatgc caaactacga gtttcgttga
     2821 gaaaagaatt gaaacgattg caaaaagaac taggaatcac tactctttat gtcacacatg
     2881 atcaagaaga agcattaaca ttatcagata ggattgctgt gtttaataat ggatttattg
     2941 agcaagttgg cacgccgtat gatatttata atcaatccgc aactcaattt gtctgtgatt
     3001 ttattggctc aatcaataaa attagccaag aagttcttaa aaaattacaa ggcgagaata
     3061 aatactttaa agaaactgaa ggatacatac gcttagagcg tgttaagtta gagaaaactt
     3121 cacctcaaga tttatctata acaggggtaa ttagtgatat tgagtttaat ggtgtactta
     3181 ttaagtataa agtagacttg tcgaatagtg aaagtattca tattatcgaa aagaatgatg
     3241 gactatcacg tcatataatc aaagaaaaag tcactatgta cattaatccg cgtgatataa
     3301 tgcaatttta aggagattgt gcatgagaaa gagtctaaac tggaaagatt gtctcataag
     3361 attaggagtt ttatggctaa tctttgccct gattatttat ccaaatattg aacttttgtc
     3421 tggagtgttt tataaaaatg gttcatttac tctagaggca ttcaataaga ttatacactc
     3481 tcagagagcg atgagtagta tatttaatag ctttattttg gcgatttcaa tggtctttac
     3541 tgtaaatatt gttgggacat tgattgttct actcacagag tactgggata ttaggggttc
     3601 aaaaatactt aaattaggtt atatgagctc tctagtatat ggaggaattg tattagcgac
     3661 aggatataaa tttgtttatg gcgctaatgg tcttgtaact aaaacgctct caagtataat
     3721 tccaggtctt gcccctaact ggtttcaagg atttggtgca gtattgttta ttatgacatt
     3781 tgcttgtacc tcaaatcaca taatgttttt aacaaatgca gttcgtagtg ttgattatca
     3841 tacaattgag gcagctaaaa atatgggtgc aagtccttta aaagttttct ttagtgtggt
     3901 ttttccaact tttaaaccta cgttatttgc cttaaccatc ttaacttttt tgacaggtct
     3961 ttcagcagtt tctgctcctt tgatagtagg ggggaaagat tttcaaacta ttaacccatt
     4021 gattatcagt ttcgctaaaa ctacaagttc gagagattta gcagctttac tagctatcat
     4081 gttaggttta gcaactattc tattattgac acttttgaat catgtcgaaa aaggaggtaa
     4141 ttatgtatct gtttcgaaaa ccaaggctag tattaagaag cagataattt cttctccatt
     4201 atggaatact cttgctcaca ttgttgcgta tgtattgttt gcaatctata tgttaccaat
     4261 agttttgata attctatact cgtttacaga ctcgttgacg atcaagacgg gaactttaga
     4321 tctatctaaa tttacgttag cgaactatgc taatttattt acagatgctc aatcatttcg
     4381 tccttaccta gtcagcgttg tttatgcaat tttagctgcg gtaattgcta caattatagc
     4441 aatagtaatt tcacgtattg tacataaaaa caagagtaag ttcgataaat tctttgagta
     4501 tggtgcactg ataccatgga tattgccagg tacattgatt gcactaggat taatgttcac
     4561 ttataatata cctcatttaa tactatttaa tttagtttta gtaggaacag taattatttt
     4621 acttattgca tatactattc aaaaattacc attttcttat agaatgattc gggctgtatt
     4681 tttcagtata gataatgata tggaagaagc tgcacgtagt atgggtgcat ctagtttcta
     4741 cactatggtg cgagttatta taccgtatat tttaccagtc gtattatcag tagttgtgtt
     4801 aaactttaat tctttattat ctgactatga cttatctgta ttcttgtacc atccactttt
     4861 ccaaccattg ggaatcgtta ttaaacagtc tactgatgaa acagcaactt taaatgcaca
     4921 ggcaatgatg tttgtttatt ctgtaatttt aatgattatg tctagtgcag ctctttattt
     4981 atcaagccta ttccaaggta aacgaggtaa gagataatat gaatctttca tacgaactga
     5041 gtatttggga aatacttctt aggtgctttt tagcgattct tttcggtggc attgttggct
     5101 atgagcgagg agtcaaaaac caagcagcag ggattagaac ctacttacta gtttgtttgg
     5161 gaacttgtgt tgtaatgatg acaaatcagt tcatcactag tcgttatggt agcggtgatc
     5221 ctagtcgaat gggagcccaa gtaattagtg gcttgggttt cctaggggca ggaacaatca
     5281 tggtaactaa tcaaaaagtt aaaggtttaa caacagcagc tggattatgg tcttcagcaa
     5341 ctattggttt agctataggc attggttttt atgaaggtgc tattttagcc ggagttgtac
     5401 tcatagcagt tttaactcta tttactcctc ttaaagatta tttaaactcg aaaactaaaa
     5461 ttgctgatta ctacttggtt tttagttctt tagaaggtta taatcgttta cttatatatt
     5521 taaatacatc gggtattaag attttagaaa ttcaaaatgg tataggaaat cttgatgaag
     5581 aacgtcaatt aaataaaatt aagtcaacaa aagtgtcctg ctatttatca ttaaaattaa
     5641 acgaaaactt tgaacattta aaattaatgg aagaaatgca taatattgct ggggttattt
     5701 atgttgaaga gattaggtaa gggtgacttt gtctaatttt ttctatattt ctaatcggag
     5761 aaaaaaatga atataatttt tgatttaggg aatgtgttga ttgaatggaa taaagaaaaa
     5821 attttatcaa aaatctgtaa gaatgattta gaatataatt tatttaataa gtttgtattt
     5881 caatctaact tgtggattga tttggataat ggtaaaatta gtttagagtt cttagagaat
     5941 caattaatcg atgaaatggg acatcaatat caggatcaaa ttcatgagtt agtatggaat
     6001 tggtttaact atgttgattt atatgatgaa gtttatgaat taataaaaca gctaaagaaa
     6061 aagaattttc aaatttatgt tttatcaaat acctcctcaa tttttcatat tttattagat
     6121 tctgttctat ctaaagtatc atctgtttta gatggttacg taatttcttg tgaagttaaa
     6181 atgatgaaac cacaaaaaga aatttacttg tcattagtta ataaatatca attagatata
     6241 aaagattgta tatttttaga tgatttagaa gaaaatgtag aagctgcaag aactctaggt
     6301 attaaagcat ttcaaattaa agagcgtaaa gagatctcaa atatactaaa agatctctta
     6361 aactagaggt taattatggt tacaataaaa gatattgcaa gagtgtctgg agtctcacat
     6421 gggacagttt caaacgtttt aaataaaaaa ggtaatgtct cagcagctaa aataaagtta
     6481 gtagaagaga ctgcgaaaaa attgggttat aatatgaatt ctcaagctca gtttcttcga
     6541 aaaggcgctt ctagtaaatg ttttttcttg ttatttaata atgcacgcaa aaagtatgct
     6601 gagtattttg cagaaattga tcagttatct ttagatgtag aatatgttta cttacataaa
     6661 ttctcagaga ttaaaaataa gatatctgca attttaagtg aaaatcctca atttatagtt
     6721 tgcttaggtt tttctccaaa aagattcgtt gatattgaag ataatattcc gatttttgaa
     6781 gtagatacat atcaatctaa taaaacttta tcttcctttg atagtgataa aatatttgag
     6841 attgtacttg agttggttcg gactgaaaag ctatctaatt tatattttat ttcacttact
     6901 aatgaaaaag aacattcttt atttgaatat ttgactcaaa aaataaaaaa ctcaactcat
     6961 atacaagtgc aagaacgttt acaattgttg tctctatatc ctactctaaa aaactttgca
     7021 aagtatgatt gtatatttgt tgatgatgaa gatttgatgc acacgctatt agacttattt
     7081 gattggttta ctataattga tagaccaaaa atatgtttga taggttcacg tgtttggttc
     7141 gaaaaaagaa atctttttta tatacattta gattatagaa tattggcact caatactgcc
     7201 gaaaatttag aaagtggaaa tagttcaatt gtaccagtta aagatggcaa acaaatttta
     7261 actaatcaat tatttgatgg agaatgtgat gatatttatt tacttactgt ccattcacca
     7321 atgaataaag ccttagaaat tttatcagaa aagtataaac aaatttctgg gaaaaatatt
     7381 aaaattatcg agaaaagata tgatgaactt ttggagatga ttgagtctgg cgatgtttca
     7441 tccagttttg atatgattag aatggatatg gcttggcttc caacatttgg gaaaaagtat
     7501 tttcaagaga ttacaagctt tagacaaaca aaaagtatta atcaaaatat ttctaagtcg
     7561 gtgtctcgag agtatatgta tattgatcag aagcaatata cttttccatt ggatgttagt
     7621 agtcagattt tagtatatag aaaagactta tttgaagatt catttttaca aagacgatac
     7681 tttgaaaaaa caaaaagaaa attaactgta ccaaaaagtt atcaagagtt tgatgagtta
     7741 agtgaatttt ttacattaac agaaaatcca ttttcaccta cactttatgg ccatagttta
     7801 gctttaagtt cttcagtgag agcagcatgt gaatttattc ctcggtttcg tgagagatta
     7861 gcacaatcta gaatgaatct tgctgtactt cctcaggttt taaccgagta tgagaaaagt
     7921 tatcagttta ccgaaaaatc atttaattct tcgtggaacg atttcgttac gaacttaaac
     7981 agtggtaaaa cttcaatgga aataattttt tcaaattata ctagtccatt atttgatggt
     8041 ctcaatgttt ctgctcagtt tgagtttgca actgccacta tacctggtaa tactcctgtg
     8101 ataggtggtg gctcaattgg aatttctaaa tattcaaata gagtggaaga atgtctaaac
     8161 tttattaatt ggttatattc tgaagagatt tctattttac taacatctct gggtggtttt
     8221 ttaccatcaa agtatgtcat gcaaaatagg atgttacaat ttcaatatcc atggttatct
     8281 agtttagaag atacatttag tattgggagt agaacaaaat ggtacggctt taatactgat
     8341 tttagatttg agcagatgtt agggcaagag ttaatagaaa gtgtgaagca tagatatgga
     8401 taaattaata ctaaattttt attactagtt tatttattat tgtttaaaat taaagattca
     8461 agtaaacacc aattatattt aaaattatta ttttggcatt tttctattgc aaagttattt
     8521 tattagactc cctgcgaaac aaaatatggt atagtagttc tatgaattat gaagcaagta
     8581 aacaattaac tgatacacga tttaagcgtc ttgttggtgt tcagcgtacc acttttgaag
     8641 agatattagc tgtattaaaa acagcttatc aaaaaa
//
LOCUS       DAVAAL010000075         8595 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVAAL010000075 DAVAAL010000000
VERSION     DAVAAL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.076"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            153..1172
                     /gene="galE"
                     /locus_tag="VQM60_001402"
     CDS             153..1172
                     /gene="galE"
                     /locus_tag="VQM60_001402"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET1081266.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            1174..2139
                     /locus_tag="VQM60_001403"
     CDS             1174..2139
                     /locus_tag="VQM60_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET1081267.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFLYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(2190..2396)
                     /locus_tag="VQM60_001404"
     CDS             complement(2190..2396)
                     /locus_tag="VQM60_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET1081268.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            2448..2924
                     /locus_tag="VQM60_001405"
     CDS             2448..2924
                     /locus_tag="VQM60_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET1081269.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            2933..3604
                     /locus_tag="VQM60_001406"
     CDS             2933..3604
                     /locus_tag="VQM60_001406"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET1081270.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLVVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            3735..4073
                     /locus_tag="VQM60_001407"
     CDS             3735..4073
                     /locus_tag="VQM60_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET1081271.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     ELKSEFVRKI"
     gene            4076..4705
                     /locus_tag="VQM60_001408"
     CDS             4076..4705
                     /locus_tag="VQM60_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET1081272.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            4945..6096
                     /locus_tag="VQM60_001409"
     CDS             4945..6096
                     /locus_tag="VQM60_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET1081273.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            6408..7157
                     /gene="truA"
                     /locus_tag="VQM60_001410"
     CDS             6408..7157
                     /gene="truA"
                     /locus_tag="VQM60_001410"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET1081274.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVAADFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            7147..7920
                     /locus_tag="VQM60_001411"
     CDS             7147..7920
                     /locus_tag="VQM60_001411"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET1081275.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNVGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            7898..8359
                     /locus_tag="VQM60_001412"
     CDS             7898..8359
                     /locus_tag="VQM60_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET1081276.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNDWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         2428 a         1605 c         1932 g         2630 t
ORIGIN      
        1 atggcaaggc gacgttgacg tggtttgaat tggattttcg aagagtataa gcattttact
       61 gaaattttat caggtagttt aggatggcaa atgacattcc ctgtcaaaaa tggtaaaata
      121 agaaaaaata atctgagaat cgaggaaaaa agatgcaaga aaagattttg gtaactggtg
      181 gtgccggttt tattggaacc cacactgtta ttgaattgat ccaagcaggc catcaagttg
      241 ttgtggtgga taatcttgtc aatagcaatc gtaagagttt agaagttgtt gaaagaatca
      301 caggagttga gattcctttc tatgaggcag atattcgtga cacagctacc ctcagagata
      361 ttttcaagca ggaagaaccg actggtgtca ttcactttgc tggtttgaag gctgttggcg
      421 aatcaacacg tatccctctt gcctactatg acaacaatat cgctggaact gtcagccttt
      481 tgaaagccat ggaagaaaac aactgtaaaa acatcatctt cagttcttct gcgacagttt
      541 acggggatcc gcacacagtg cccatcttgg aagatttccc actttcagtg accaacccat
      601 acggtcgtac taagctcatg ctagaggaaa ttttgactga tatttacaaa gcagactcag
      661 aatggaatgt tgtcttgctt cgttacttta acccaatcgg agcccatgag agtggtgatt
      721 tgggagaaaa tccaaacggt attccaaaca atctcttgcc atatgtgact caagtagccg
      781 ttggaaaatt agagcaagtg caagtgtttg gagacgatta cgatacggaa gatggaacag
      841 gtgttcgtga ctatatccac gttgtcgatt tggctaaggg tcacgttgca gctttgaaaa
      901 aaatccaaaa aggttcagga ctaaacgttt ataaccttgg aactggtaaa ggttactcag
      961 ttcttgaaat tatccaaaac atggaaaaag cggtgggacg tcctattcct taccgtatcg
     1021 tagaacgtcg cccaggtgat atcgctgcct gctactcaga cccagcaaaa gctaaagcag
     1081 aactcggttg ggaagcagaa ctcgacatca cccaaatgtg tgaagacgca tggcgttggc
     1141 aaagcaagca tccaaatgga tttgaagact aagatgatga tttcaatcat cgtcccttgt
     1201 ttaaacgaag aggaagtact tcctcttttt tatcaggctc tggaagcttt acttccagat
     1261 ttggaaacag aaatcgagta tgtctttgtc gatgatggat caagtgatgg gaccttggaa
     1321 ctcttaaagg cctatcggga gcaaaatccg gcagtccatt atatttcttt ctctcgaaat
     1381 tttggcaaag aagcagccct ttatgcaggc ttgcaatatg cgacaggaga tttggtggtg
     1441 gtgatggatg cagacctcca agatcctcct agtatgttgt ttgagatgaa aaatgtacta
     1501 gacaaaaatg tagacttgga ctgcgttggg acacggagaa ctagtcggga gggagaaccc
     1561 ttctttcgca gtttctgtgc tgttctcttt tatcgcctca tgcaaaaaat cagcccagtg
     1621 gctctgccgt cgggtgtccg tgattttcgt atgatgagaa ggtctgtagt cgatgccatt
     1681 ttaagcttga ccgagtccaa tcgtttttca aaggggctct tcgcctgggt gggtttcaaa
     1741 acctactatt tggactatcc aaatgtcgaa aggcaggctg gcaagacaag ttggagtttt
     1801 aggcaactct ttttatactc cattgaaggg attattaatt tttcagattt tcccttgatt
     1861 atagcctttg tagctggtct cctatcttgt tttctttctc tgctgatgac cttttttgtt
     1921 gtggttcgga ccctcatttt gggcaatccg acatctggtt ggacctctct gatggctgtt
     1981 attctctttc ttggaggcat tcaactcttg accattggga ttctcggcaa gtatatcagt
     2041 aagatttatt tagagactaa aaaaagacca ctttatctta tcaaagaaaa aagtgacctt
     2101 cctgatttta cagaaaaaaa taaagtgaaa agactataat tttacatgga aatgtgctaa
     2161 actagaggga gtggattatc gccattgatt taggcttcct ggtttccaat cagggcgcga
     2221 gttgggcaat ttttaacagc ctctaagaca tcctgactag gagaaatttc tttttccagt
     2281 tggtcagggt catcgtaaaa acgcacgatt ccattatcgt ggtaatcaaa taaatcagaa
     2341 taagtttggc aaagcccaca ggcgatacat cgttcaggta taagtgtgat tttcatgttc
     2401 atatttatat tgtaataaga aaagtaaaaa aaaacaagga gtaaggtatg gcaaaagaac
     2461 cgtggcaaga agatatctat gatcaagaag aatcaagagc agagcgtcgg catcgaaacc
     2521 acggaggggc tgataggatg gctaatcgta ttttgacgat cctagctagt attttctttg
     2581 taattgtggt ggtgatggtc atcgttctca tctatctatc atcggggggg agtaatcgca
     2641 cagcagcctt aaaagacttt catgattctg atgcaagtgt agtacaaatc tcatcttcaa
     2701 gcagttctca gcctgagcag agttcagagc cagaatctac ttctagtagt tcagaagaag
     2761 ctgctaatcc tgaaggaacg attaaagttc tcgcaggaga aggggaagca gctattgccg
     2821 ctcgtgcagg aatctccatt gctcagttag aggccttgaa tcctgggcac atggctacag
     2881 gatcttggtt tgctaatcca ggtgatgtta taaaaataaa ataggagccg atatgaaaac
     2941 aattcaaatt gctattgatg gtcctgcttc cagcggtaag agtacggtcg caaagattat
     3001 tgctaaggat tttggattca cctaccttga tacaggagct atgtatcgtg cagcgaccta
     3061 tatggctctt aagaaccaat tagtagttga agaagtcgaa gcccttctag ccttgttgga
     3121 ccagcatcca atcagctttg gacgttcaga aactggagac cagcttgttt ttgtaggaga
     3181 tgtggatatt acccatccta tccgtgaaaa tgaagtgacc aatcatgttt ctgctattgc
     3241 agcaattcct gaagtgcgtg agaaactggt ttctctccaa caagaaattg cccagcaagg
     3301 cgggattgtc atggatggtc gcgatattgg aactgttgta ttgccacaag cagaattgaa
     3361 aattttccta gtagcttctg ttgatgagag agcagagcgt cgttacaagg aaaatattgc
     3421 caagggaatt gaaacagacc ttgaaaccct aaaaaaggaa attgctgcgc gtgactacaa
     3481 ggatagtcat cgtgagactt ctcctctcaa acaagcagag gatgctgtct accttgatac
     3541 aactggtttg aacattcaag aagtagttga aaaaatcaaa gcagaagctg aaaaaagaat
     3601 gtaaatgtag aaaagccgat agattgatat cggttttttt ctaaatttgt caaaatgatg
     3661 attttagggt ataatagttg ctgttcgaga aattttgatt ttcaaagaat agtgaatgat
     3721 gataaggaga aaccatgaac aatttaccaa attgtccaaa atgtaactca gagtatgtct
     3781 acgaagacgg tgccctactg gtttgcccag agtgtgctca tgagtggaat cctgctgaag
     3841 ttgcagaagt agaagaaggt cttgtcgcta tcgatgccaa cggaaataaa ttgactgatg
     3901 gtgatacagt aactctcatc aaggacttga aagtaaaagg tgcgccaaaa gatttgaaac
     3961 aagggacgcg cgtgaaaaat atccgcatcg tagaaggcga ccacaatatc gactgtaaga
     4021 ttgatggttt tggtgccatg gaacttaaat cagagtttgt gaggaagatt taagcatgtc
     4081 aaaacactat aaacttgtat tttatagccg tatcttcttg tttctagcgg cttttacggg
     4141 agtttatctt gaaattacta agcatggtgg ttttgggatg cttctctatt acacggttct
     4201 gtccaacctc ttggtaacta tttttaccct ttatcttcta aaggttatga gccgtgtagg
     4261 tgaaaattgg caaaggccaa gtctcttgcg cttaaaaggt ggggtcacca tgagtatcat
     4321 gattacctgt gtgatttacc atttcctctt ggcgcccatt gcgactaatt tctataccct
     4381 agaaaatttc ctttgccact atatcgttcc catctggttt ttagcggata ccctcttttt
     4441 tgacaaacag ggtcaataca agatttggga tccagcagtg tggaccattt taccctttct
     4501 gtatatgatg tttgctcttt ttaatggctt ggttctaaaa ctcaatattc caaatgctaa
     4561 ggataatcct ttcccttact tctttttgaa tgtgaacaag ggttggaatg ttgtgtttaa
     4621 gtggtgtctg attatctttg ttgcctatat ggtagcagga tttattttct actttatcaa
     4681 gcaaatcaag agaaagtcat cctaatactc ttcgaaaatc tcttcaaacc acgtcagttt
     4741 ccatctgcaa cctcaaaaca ctgttttgaa caacctgcgg ctagcttcct agtttgctct
     4801 ttgattttca ttgagtataa gatacaaggg attcattttc gaatttaaga aggagtctac
     4861 aaaccaatga ggcttcttct tttcttgctt atttgataac tatcaaaaaa gtagaagatt
     4921 aagagaaata tagaaagaga tttcatgaaa caatttttag aacgggccag cattttggct
     4981 ctctccctcg ttttgattac ctccttttcc atttcgagtg ccctaccagc catgtttgac
     5041 tattatcagg gttattctaa ggaacaaatt gagctcttgg tgagcttgcc ttcctttgga
     5101 atcatgatga tgttactgct aaatggtttc ttagaaaaaa tatttcctga gcgcttacag
     5161 attagtttgg gcttgctgat tttatcattg agcggtacag ctcccttctg gtaccaagcc
     5221 tatccctttg tctttggaac acggcttctc tttggtttgg gtcttgggat gatcaatgcc
     5281 aaggccattt ctattatcag tgaacgctac caaggaaaaa ggcgaattca gatgttaggg
     5341 ctacgcgctt ctgcagaggt cgttggagct tctctcatta ccttggccgt cggtcagttg
     5401 ttggcctttg gttggacagc tatctttcta gcctatagtg ctggattttt ggtgctgccc
     5461 ctttatctgc tctttgtccc ttatggaaaa tcaaagaaag aagtcaagaa aagagcgaag
     5521 gaagcaagtc gtttaactcg agaaatgaaa ggcttgattt ttaccttagc tatcgaagcg
     5581 gcagttgtag tttgtaccaa tacagctatt accatccgta ttccaagttt gatggtggaa
     5641 agaggattgg gggatgccca gttatctagt tttgttctta gtatcatgca gttgatcggg
     5701 attgtggctg gggtgagttt ttctttcttg atttctatct ttaaagagaa actgctcctc
     5761 tggtctggta ttacctttgg cttggggcaa atcgtgattg ccttgtcttc atccttgtgg
     5821 gtggtagtag caggaagtgt tctggctgga tttgcctata gtgtagtctt gacgacggtc
     5881 tttcaacttg tctctgaacg aattccagct aaactcctca atcaagcaac ttcatttgct
     5941 gtattaggct gtagtttcgg agcctttacg accccattcg ttctaggtgc aattggctta
     6001 ctaactcaca atgggatgtt ggtctttagt atcttaggag gttggttgat tgtaatctct
     6061 atctttgtca tgtacctact tcaaaagaga gcttaggatt gattcctaag tttttctttt
     6121 gatactcaat gaaaatcaaa gagcatagtt gattgagttt ggaatagtac gctgtagctg
     6181 ctaaaatatt tctagaaatt aatttgactt tcctaataga gttgttcata tcttatttca
     6241 atctactata gaaagctagc cgcaggttgc tcaaaatagt gttttgaggt tgcagatgga
     6301 aactgacgtg gtttgaagag attttcaaga gtataagatt tttataccca tacaatattt
     6361 gttttaggtc cttgtttaca gactaatttc ttgataaaat agaggttatg acgagatata
     6421 aagcaactat ttcctatgat ggttatgcct ttgctggctt tcagcgccag cctcatgcgc
     6481 gtagcgttca ggaagaaatt gaaaaaacct tgaccagatt aaataaaggg caaaccatta
     6541 ctgttcacgg tgctggtagg acagatagtg gggttcatgc cctgggacag gtcattcatt
     6601 ttgacctgcc ttatcagatg gatgaggaga aactccgttt tgccttggat acccagtctc
     6661 ctgaagatat tgatgtgatt tcgattgagc ttgtggcagc tgattttcat tgccgttatg
     6721 ccaagcatag caagacctat gagtttattg tggatagagg acgtcccaaa aatccgatgc
     6781 gccgtcacta tgccacccac tttccctacc cactcgatgt ggaacgaatg cagattgcaa
     6841 tcaaaaagct agagggaacc catgatttta ccggttttac agcctctggg actagtgtag
     6901 aggataaggt tcgcaccatc acagaagcta gtttaatagt cgatgagaca ggacaatttt
     6961 tgacctttac cttttccgga aatggtttct tgtataaaca gattcgcaat atggtgggga
     7021 ctcttctcaa aatcggtaac aaccgcatgc cagtagagca gattgatctc atcttggaga
     7081 agaaggacag gcaacttgca ggtcccactg cagcaccaaa tggtttgtat ttaaaggaga
     7141 ttcgttatga agaataatcg tattttagca ctttctggaa atgatatttt tagtggtggt
     7201 ggactgtcag ctgatttggc tacctatacc ttgaacggct tgcatgggtt tgtagcagtg
     7261 acttgtttga cagccttgac agaaaaagga tttgaagtct ttccaactga tgataccatt
     7321 tttcaacatg aattagatag cttgcgtgat gtggaatttg ggggaattaa gattggtctt
     7381 ctccctactg tcagtgtggc tgagaaggcc ttggacttta tcaaacaacg cccaggagta
     7441 cctgtggtgt tggatcctgt cttggtctgc aaggaaacgc atgatgtagc tgtcagtgag
     7501 ctctgccaag agttgattcg ctttttccct tatgtcagtg tgattacgcc taatctccca
     7561 gaagcagaat tattatccgg tcaggaaatt aaaaccttgg aagacatgaa aactgcagcg
     7621 cagaaattgc atgatttagg agcgccagca gtcattatca agggaggcaa tcgtcttagt
     7681 caggacaagg ctgtggatgt cttttatgat ggacagacct ttactatcct agaaaatcca
     7741 gttatccaag gccaaaatgt tggtgcaggt tgtacctttg cctctagcat tgccagtcac
     7801 ttgattaaag gtgataaact tttgccagca gtagaaagct ctaaggcttt cgtttatcgt
     7861 gctattgcac aagcagatca gtatggagta agacaatatg aagcaaacaa aaacaactaa
     7921 aatcgccctt gtatccctat taaccgccct ttctgtggtt ctaggttatt tcttaaaaat
     7981 cccaacacct acaggaattc taactctttt agatgctggt gtcttctttg cggcctttta
     8041 ctttggtagt cgtgaaggag cggtagtcgg aggactagca agtttcttga ttgacctctt
     8101 atcaggctac cctcagtgga tgttctttag cttggtcaac catggcttgc agggattttt
     8161 cgcaggattt aaaggaaaaa gtcagtggtt aggccttatt ttagcaacta ttgccatggt
     8221 aggaggctac gccttgggtt ctactttgat gaatgactgg gcagcagccc tcccagaaat
     8281 tctaccaaat tttatgcaaa atatggtagg gatgattgta ggatttattc ttagtcaaag
     8341 tatcaagaag attaagtaat actcttcgaa aatctcttca aaccacgtca gcttccatct
     8401 gcaacctcaa aacactgttt tgagcagcct gaggctagct ttctagtttg cactttgatt
     8461 ttcattgagt ataaagaggt tgagtaaaaa gtatttcaaa taagaaaaac gcatagtata
     8521 ggatgttgaa atgatgaact gccccccaaa agttagacag aaaaaatcta acttttgggg
     8581 tgttttatta tgaaa
//
LOCUS       DAVAAL010000076         8444 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVAAL010000076 DAVAAL010000000
VERSION     DAVAAL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.077"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            91..888
                     /locus_tag="VQM60_001413"
     CDS             91..888
                     /locus_tag="VQM60_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET1081277.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1036..1158
                     /locus_tag="VQM60_001414"
     CDS             1036..1158
                     /locus_tag="VQM60_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HET1081278.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1180..2220
                     /locus_tag="VQM60_001415"
     CDS             1180..2220
                     /locus_tag="VQM60_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HET1081279.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILAIAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFFDIDNLEEVEAAIKDNTKLVLIETLG
                     NPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSVTKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2391..3665
                     /locus_tag="VQM60_001416"
     CDS             2391..3665
                     /locus_tag="VQM60_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET1081280.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3675..4553
                     /gene="truB"
                     /locus_tag="VQM60_001417"
     CDS             3675..4553
                     /gene="truB"
                     /locus_tag="VQM60_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET1081281.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4794..5092
                     /locus_tag="VQM60_001418"
                     /pseudo
     CDS             4794..5092
                     /locus_tag="VQM60_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
     gene            5200..5838
                     /gene="udk"
                     /locus_tag="VQM60_001419"
     CDS             5200..5838
                     /gene="udk"
                     /locus_tag="VQM60_001419"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET1081282.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5966..7306
                     /locus_tag="VQM60_001420"
     CDS             5966..7306
                     /locus_tag="VQM60_001420"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET1081283.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGNYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7284..7496
                     /locus_tag="VQM60_001421"
     CDS             7284..7496
                     /locus_tag="VQM60_001421"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET1081284.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7493..8368
                     /locus_tag="VQM60_001422"
     CDS             7493..8368
                     /locus_tag="VQM60_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET1081285.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8361..>8444
                     /locus_tag="VQM60_001423"
     CDS             8361..>8444
                     /locus_tag="VQM60_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HET1081286.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMA"
BASE COUNT         2518 a         1535 c         1991 g         2400 t
ORIGIN      
        1 tactataaac ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa
       61 ttatcaagaa aaaagattta ggagtttatt atggtttctt cggaatttat ctcaaagatt
      121 gaatttgctt gcaataagaa agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt
      181 cgttcgatgt tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct
      241 gacttgatta ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct
      301 tggggcttgg cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc
      361 ttgactgctg gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta
      421 tactgtacct tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg
      481 gcagcctatg cgaatctgac acacgatagt ttcatctcag gtgttgttga gatgaagtta
      541 ggccgctcaa atgaattggt cttgcttgag gcgattttgg caaatatttt tgtaaatatt
      601 gcgattctgt catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca
      661 gctatttaca tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc
      721 gcgattgtga aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg
      781 cttcgccact ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt
      841 cttccatatg ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac
      901 gattgagtcg tgcttttttc attttcaaaa taaggtaata gctatttctt atatcaaaat
      961 atagaaaact gatatttgta aactataact caaggtgcta caatatcctt aataaaataa
     1021 tatggaggtc accttatgac ttgtgatttt aaatttgaaa ctctacaact acatgctggt
     1081 caagttgtgg ctccagctac taagtctcgt gcagtgccga tttatcaaac aacatttttt
     1141 gtttttgatg acacgtagga aggtgccgat ctgtttgcct tgaggaaacc agggaacatt
     1201 tatactcgta tcaccaatcc tacaacagct gcccttgaag gtggtgttga agcgctagca
     1261 acagcatcag gtatgactgc agtgacttat actattttgg cgattgccca tgctggtgac
     1321 catgtagtgg ctgcttcgac tatttacggt ggaaccttca atcttttgaa agaacccctt
     1381 cctcgttatg gtatcacaac aacctttttc gatattgata atttggagga agtagaagca
     1441 gctatcaaag acaataccaa gcttgtcttg attgaaacct tgggtaaccc cttgattaat
     1501 attccagacc tggaaaaact ggcagagatt gctcataaac atcaaatccc acttgtgtca
     1561 gacaatactt ttgcaacacc ttatttgatt aacgtcttct ctcatggcgt tgacattgcc
     1621 attcactctg tgactaagtt tatcggtggg catggtacaa ctattggagg aataattgtc
     1681 gatagtggtc gttttgactg gacggcttca gggaaattcc ctcaatttgt tgacgagggt
     1741 ccaagctgcc acaatttgag ctatactcgt gatgtgggtg cagcagcctt tattatagct
     1801 gttcgagttc aattgcttcg tgatacaggt gcagccttgt caccattcaa tgctttcctc
     1861 ttgctacaaa gacttgaaac ctcttcactt cgtgtggaac gccatgtaca aaatgctgag
     1921 acaattgttg attttcttgt caaccatcct aaggtagaaa aggtaaatta tccaaaactt
     1981 gcagatagtc cttatcatgc cttggctgag aaatacttgc caaaaggtgt cggttcaatc
     2041 tttaccttcc acgtcaaagg tggcgaggaa gaagcacgca aggtcattga taatttagaa
     2101 atcttttctg accttgcaaa cgcggcagat gctaaatcgc ttgttgtcca tccagcaaca
     2161 accactcacg gtcaattgtc agaaaaagac ctagaagcag caggtgtcac accaaactaa
     2221 attcgtttgt caatcggtct tgaaaatgta gaagatttga ttgaagactt gcgcttggcc
     2281 ttggaaaaaa tttaaagtaa aagaagataa acagtgggct tcgactcact gtttttgatt
     2341 ttccctcagg catgatataa tggttaaaga agtctagaaa gaggaacgat atgaacgaaa
     2401 tcaaatgtcc caactgtggg gaagtcttta cagtaaatga gagtcagtat gccgaactct
     2461 tgtcccaagt gagaacggca gagtttgata aggaactaca cgataggatg aagcaggaac
     2521 tggccttggc tgagcaaaag gccatgaatg agcaacagac taaactggct cagaaggatc
     2581 aagaaattgc gcaattacag agtcagatcc aaaactttga tacagaaaaa gaattggcca
     2641 agaaagaggt tgaacagaca agccatgagg ctctcttggc taaggacaag gaagtacagc
     2701 tcttagaaaa tcagttggct accttgcgtt tggagcatga aaatcaacta caaaagaccc
     2761 tttctgacct agaaaaagaa cgggatcagg ttaaaaacca actacttttg caggaaaagg
     2821 aaaatgaatt atctttggct tctgttaagc aaaactacga agcccagctc aaggcagcta
     2881 gtgaacaagt cgagttttat aagaatttta aggctcaaca atctacaaaa gcgattgggg
     2941 aaagcctaga acagtatgca gagagtgagt ttaacaaggt tcgtagtttc gcctttccaa
     3001 atgcttactt tgagaaggat aacaaggtct cttcgcgtgg gtctaaaggg gactttatct
     3061 tccgtgagtg tgatgaaaat ggagttgaaa tcatttctat catgtttgag atgaaaaacg
     3121 aagcggacgg aacagagaag aagcacaaga atgcagattt ttacaaggaa ttggacaagg
     3181 accgtcggga gaagaactgt gagtatgccg ttttggtgac catgcttgag gctgataatg
     3241 actactttaa cacagggatt gttgacgtca gtcacgagta tgaaaaaatg tatgttgttc
     3301 gtcctcaatt ctttatccaa ttgattggtc tcttacgtaa tgcggcgcta aattccctaa
     3361 aatacaagca ggagttggcc ttggttcgcg agcaaaatat tgacattacg cattttgagg
     3421 aagatttgga tgcctttaag ctagcttttg ctaagaacta taattcagct tcgactaact
     3481 ttggaaaagc tattgatgaa atcgacaagg ccatcaaacg catggaagag gttaagaaat
     3541 tcctgaccac atctgaaaac caactccgtt tagctaacaa caaattggaa gatgtctctg
     3601 ttaaaaaatt gacccggaaa aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg
     3661 agtagaaagc aaaaatgaac ggtattatta acttaaaaaa ggaagcagga atgacctcgc
     3721 atgatgcggt ttttaaactg cgtaagattt tgggaaccaa gaaaattggt catggtggaa
     3781 ccttggatcc ggatgtggtg ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg
     3841 tcgagtttat gcaggacgag ggtaagatct atgaggggga aatcactctg ggctattcca
     3901 cgaagactga ggatgctagt ggggaagtgg tcgcagaaac ccctgttttg tctctcttgg
     3961 atgaaaagct tgttgatgaa gcgattgcta gcttgactgg gcctattact cagattcccc
     4021 ctatgtattc ggcagttaag gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc
     4081 aggaagtgga gcgtccagaa cgtcaggtga ccatttatca atttgagcga acaagtccga
     4141 tttcttatga tggccaactt gcccgattca cttttcgtgt aaaatgcagt aaagggacgt
     4201 acatccgtac tttgtcagtt gatttgggtg aaaagcttgg ttatgcggct catatgtccc
     4261 atttgactcg tactagtgct gctggcttac aattagaaga cgctcttgcc ttggaggaaa
     4321 ttgctgaaaa agtagaggct gggcaattag attttctcca tcctttagag attgggacag
     4381 gtgaccttgt caaagttttc ctaactccag aagaggctac agaagttcgc tttggtcgtt
     4441 ttattgagct agaccaaacg gacaaagaac tggctgcctt tgaagatgat aaattgttag
     4501 ccattctaga aaaacggggc aatctctata agccaaggaa ggtttttagc tagatcgttt
     4561 aggaataaaa atcgggtgat agataacaat tgcttgataa aaccccatac taatagtaga
     4621 atggttttgg gaattataat attccaattg ttgcgagttg taggtactca aataatctat
     4681 atagaaattt agaggtgtga aatgaagcaa tttaaaattc tttcagataa atatttagag
     4741 tccattacag gttctgatgg gaacttaggc ccaggatttg gtgtgataat tccatgatgc
     4801 gaaatgagtt tcgagaaagg gtggagcaac ttcttcaaca aaaagaaata aatgaaaata
     4861 gtgagttgag tcacctgttt cgtcttgcta tacaaaattt agacagaaat gaaaaatacc
     4921 aatcggtcat ggccaatttg agtcaagggt tgtcacttta cctcatgacg catcattacc
     4981 aggcacctaa gtctgtcatt gattttggtt tatggattaa gctcctagcc aggaaagagg
     5041 gagactggct ttcttgcaga tgcttgctca aaccctacag ggctttcgtt aaaactgtcg
     5101 aacatcagag ggaagactct ggtgtttttt gctgatgaga ctataaattt tgtaaaaaat
     5161 gtgatagaat agacgacgga taaaataacg gaggatagca tgcaaaatag accaatcatt
     5221 attggagtga caggtggttc tggtggtggt aaaaccagtg tttcaagagc cattttatcg
     5281 cattttcctg atgaaaagat ttccatgatt gagcatgatt catactacaa ggatcagtct
     5341 catttgacct ttgaagagcg tgtcaaaacc aactatgacc atccttttgc ctttgataca
     5401 gacttgatga tcgagcagat taaggaattg ttggcagggc gtccggtgga catcccgacc
     5461 tacgactata cagagcatac acggagtagc aagacctatc gtcaggaacc tcaagatgtc
     5521 tttatcgttg agggtatttt ggtcttggag gacaagcgtc tgcgcgattt gatggatatc
     5581 aagatttttg tggatacgga tgacgatgtg cgcattattc gtcgtatcaa gcgtgatatg
     5641 gaggagcgtg gccgtagcct tgatagcgtt attaaccagt acttaggtgt ggtcaaacca
     5701 atgtaccacc agtttatcga gtcaactaag cgttatgctg atatcgtcat tcctgaaggg
     5761 gttagcaata ccgtggctat cgacctgttg acgaccaaga ttgcaaagat tttggaagaa
     5821 gctcgaaaca gcaaataatc acatgtggag gccttgcctc ctttttctat ttttccctca
     5881 taatggtaca taaatggaga tttttaggca tatttttggt ataataatac ccatgaaaga
     5941 gcaagtaaat gaatagtagg tggagatgga aaagtattta tcggtaacaa ctttgaccaa
     6001 gtatctgaaa atgaaattcg ataaagaccc atacttggaa cgggtctatt taactggtca
     6061 agtttccaac tttcgtaaac gacctactca ccaatatttc tccctaaagg atgaccatgc
     6121 agttattcaa gcgaccatct ggtctgggat ttatcagaaa ttagggtttg acctggaaga
     6181 aggaatgaag atcaatgtga ttgggcgtgt acaggtctat gaaccaagtg gtaactactc
     6241 catcatcatt gaaaaagctg agcctgatgg ggttggggcg cttgcgattc agtttgaaca
     6301 acttaagaaa aaattgacag aagaaggcct gtttcaagaa cgcttcaagc aagctctgcc
     6361 ccaattttct aagagaattg gtgtagtaac cagccgtagt ggagccgtta ttcgagatat
     6421 tatcacgacc gtcagcaggc gattcccagg tgttgacatc cttctttatc cgaccaaggt
     6481 tcaaggtgaa ggggctgcgg aggaaattgc tagaaatatt gcgcgtgcta atcaacggga
     6541 cgatttggat ttgctcatta ttggtcgtgg aggtggttct attgaggatc tctgggcctt
     6601 taacgaagaa attgtggtac gagctatttt tgaatctcgt ttgccagtta tctctagtgt
     6661 ggggcatgag acggatgtga ccttggcaga ttttgtggca gatcgacgcg ctgcaacgcc
     6721 aacagcggcg gctgaactgg caacacctgt gaccaagttg gatgtattag ctcatttgca
     6781 aaatcaggaa aaacggatgg taacggcagt ccgaaatgtt ctatctaaga aacaagaggc
     6841 tttgaaaaaa tgcagtcagt ctgttatctt tagacaacct gagcgcttgt atgacggtta
     6901 tttgcaacgc ttggaccaac tgcaactgcg tttgaaacaa agtttgcgaa ctcggatttc
     6961 tgataacaaa caattagttc aagcaagaac tcatcaatta gtacaattat cacctgttac
     7021 caaaatccaa cgctatcaag accgtttagg acagttggac aagctcttag gtagccaaat
     7081 ggcgttagtt tatgacgcca aggttgctga ggccaagcga ctttcggaag ctttgctcat
     7141 gttggatact agccgaatcg tggcgcgtgg ttatgctatt gtcaaaaaag aagagtccgt
     7201 tgtagattcg gttgagagtt tgaagaaaaa agaccaagta acgcttttga tgcgagatgg
     7261 tcaagtagaa ttagaggtta aagatgtcaa aacaaaagaa atttgaggaa aatctagcag
     7321 aactggaaac cattgtccaa agtttggaaa atggtgaaat tgctctggaa gatgcgatta
     7381 ctgcctttca aaagggcatg gtcttgtcaa aagagctcca agctacgctg gacaaggctg
     7441 aaaagacctt ggtcaaggtc atgcaagaag acggaacaga aagtgatttt gaatgaaaaa
     7501 gcaagaaaaa ttagctcttg tcgagtcggc tttggaagat ttttatggag accagcagtt
     7561 tgcctctagt ttacgggagt ctgttctcta ttctattcat gctggtggca agcgtattcg
     7621 gccttttctc ttgttagaag ttctggaagc cttgcaggtt accatcaaac ctgctcacgc
     7681 gcaggtagct actgccttag agatgattca tacagggagc ttgattcacg atgaccttcc
     7741 tgctatggat gatgacgatt atcgaagagg gcggttaacc aatcacaaga aattcggtga
     7801 agctatggcc attttggctg gagatgcctt attcttagac tcatatgcct tgattgcgca
     7861 ggcagatttg ccaagtcaga ttaaggtgga cttgattgcc aacttatccc ttgcttcagg
     7921 tagtctgggt atggtggcag ggcaagtttt ggatatggag ggcgaacacc agcacttgtc
     7981 tttggaagaa cttcagacta ttcatgccaa taagactggg aagttactag cctatccctt
     8041 ccaagcggca gctattatag ctgaattgtc acctgaaatg caggtgaagc tgaaaactgt
     8101 gggtgaattg attggacttg cttttcaagt cagagatgat gtactggatg tgacagctag
     8161 ttttgaggaa atcggcaaga cacctcaaaa ggatctgcag gcagaaaaat caacctatcc
     8221 tgccctgttg ggcttggaag agtccattgc cttttgtaac caaaccctgg atcaagctaa
     8281 tgaaaaatta gaagaaattg cccagcagct tccctttgaa acagaatcga ttgtaagtgt
     8341 agtagaaagt ttgagaatca atggctaagg aaagagtgga tgtactagct tataaacagg
     8401 ggttgtttga aacgagagag caggccaagc gaggtgtgat ggct
//
LOCUS       DAVAAL010000077         8376 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVAAL010000077 DAVAAL010000000
VERSION     DAVAAL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.078"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(82..954)
                     /locus_tag="VQM60_001424"
     CDS             complement(82..954)
                     /locus_tag="VQM60_001424"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET1081287.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            1191..2501
                     /locus_tag="VQM60_001425"
     CDS             1191..2501
                     /locus_tag="VQM60_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET1081288.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            complement(2867..3061)
                     /locus_tag="VQM60_001426"
     CDS             complement(2867..3061)
                     /locus_tag="VQM60_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET1081289.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(3522..4250)
                     /locus_tag="VQM60_001427"
     CDS             complement(3522..4250)
                     /locus_tag="VQM60_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET1081290.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            4408..5817
                     /locus_tag="VQM60_001428"
     CDS             4408..5817
                     /locus_tag="VQM60_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET1081291.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            5835..7130
                     /locus_tag="VQM60_001429"
     CDS             5835..7130
                     /locus_tag="VQM60_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET1081292.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            7135..7443
                     /locus_tag="VQM60_001430"
     CDS             7135..7443
                     /locus_tag="VQM60_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET1081293.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            7440..7748
                     /locus_tag="VQM60_001431"
     CDS             7440..7748
                     /locus_tag="VQM60_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET1081294.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         2715 a         1588 c         1467 g         2606 t
ORIGIN      
        1 ttgaagaggc gtttaaacta tctgacataa aactcgttct agaggaaaga catcctttaa
       61 aaagttagtt tattttacaa cttagacatc aaggtaggtt aaacccttca tggaaaaatc
      121 aagactctta gcactatggg ttaaactacc actggagacg taatcaatcg ctaaaccacg
      181 aaaacggcta atagtggtca tatcaatatt tccagaacat tcaatccgag aacgtcctgc
      241 aattagggta atggcctgtt caatctgttc caatgacata ttatccaaca tgataatatc
      301 agcacccgcc gccgcagctt cttcggcagc agcaaggctt tccacttcca cctcgaccat
      361 tttcacaaaa ggggcatagg cacgcgcttg agcaattgcc ttctgaacac tacctactgc
      421 cgcaatgtga ttgtctttta gcatgatagc atctgataaa ttaaagcgat gattatagcc
      481 accgccaact ctcacggcat atttctcaaa aagacgtaaa ttaggagtag tttttcgagt
      541 atcaaatacc ttaatgcaat catcgcctaa ggcttctaca taagcagctg tcatcgaagc
      601 aatccctgat aaatgttgta aaaaattcaa ggcaacgcgt tcacatgtta agagacttct
      661 caccgagcct atgatttcta aaaccaaatc gccactagtc aaacgatccc catccttaaa
      721 ttgatgagga ttctggaagg tcacctcggc atcaaatagg gtaaaaaccc tttgaaaaac
      781 ggttagtccc gctaaaacac cagcttcctt ggcaaaaagc gacaccttgg cttggccatg
      841 atgatcaaaa atggcattgg tactgtaatc ttcggaatga acatcttctc gcaaggctgc
      901 tttcaatgta tcatctattt gaaaaggggt taaatcagtt gaaatgattg acatcactaa
      961 gtcctctatt tttaatatca cttgtaagca ctagaggcaa ttactattaa cttccatagg
     1021 aaatcacaag cctgaatacc tcatttttaa ttctaacaag tgtcaataca attgtcaaga
     1081 caattgtatt ttttttgtgt ataatagtca agtaccaata ggaaacaggt ggtcttttga
     1141 gcgccaaaat tgccaaccta tttccatatt ctaactgaaa ggacaattca atgattcatt
     1201 taataatgat tagcgccatt gctctagcca ttggaattgg ttaccgcacc aaaatcaata
     1261 ttggcctgct ggctattgct ttttcttacc tcatcgcaac cactctcatg ggattaagcc
     1321 ccaaagaact tcttcatttt tggccaacct cactcttttt taccattttt agcgtctctc
     1381 tcttttataa cgttgcaaca actaacggta ctcttgatgt tttggctcaa cacattctct
     1441 accgcacacg cacccaccct aacgccctct acatgatttt atacctgatg gcaacccttt
     1501 tgtctgcttt aggtgctgga tttttcacta ctatggccgt ttgctgtcct ctagcgatta
     1561 ccctctgtca aaaagcggac aaacaccctt tgattggagc tcaagccgtc aattggggag
     1621 cttcaggagg ggctaatttg ataaccagta gctcaggcat tgtctttcaa ggcctgttta
     1681 agcaaatggg atgggaagag caagcttttt cacttggcaa tcatatcttt atcgtcagca
     1741 ttatctaccc actcatcgtc ttgcttttat tatcctgtta cagtcactat agtaagggaa
     1801 gaacaaactc atccttgact attgaccaac caccactcct cagcaaggtt caaagacaaa
     1861 ccaccttgct catgatcagc agcatggttt tggtttggct ttttcccttg ttgcatctca
     1921 ttttcccaaa catcgcctgg atagctactt atcaaaaaac atttgatatt ggctttgtct
     1981 caatactcat ggtctgcctc gccttaaggc tcaaattagg gaagcaagaa gccattttag
     2041 ctaaggttcc ctgggctaca attatcatgc tctgcggcat gagcttacta atgtctctag
     2101 cggtcaagtc aggtttagtc acactgatag ggcacctcat gacaacaacc attcctcatt
     2161 tctggttacc tctgttcttt tgtgtgatag ccggagtgat gtcactcttt agttccaccc
     2221 tatccgttgt agctccagcc ctctttccca tcattgctat catcagtgct caaaatcctc
     2281 agattgatat tcacttactc acaacagcca ccgttattgg cgctctatct actaatatct
     2341 cacccttttc atcagctggt tctctcattc aactctcact tcccaatata gaggaacgtg
     2401 gccttgcctt caaaaagcaa attattctgg gtgtccccat cagcttaagt ttgggactct
     2461 tgaccacctg gattctcatc cttctagctt ccttaagtta gatttattat ctaacttaag
     2521 ggtggacttg gtataataaa gtagacacaa aattaagtgg aaacgaggaa aagtcattat
     2581 gccaagaaaa acctttgata aagccttcaa actctctgct gtaaatctca tcctgtaaaa
     2641 atgattagtt caactttaaa aattcatccc aatagtcttt atcgatggat tcaagaatat
     2701 gaaaaatatg aagaaagtgc gttcccagga catgggagcg cacttcgtca tactcaattt
     2761 gagatatagt gaaatgaaat aagaacagga caaatagatc aggatagtca aatcgatttc
     2821 taacaatatt ttagaagtag atgtgtacta ttctagtttc aatctactat agtaaagttg
     2881 actttcctgt accattttta ccagtaatta atgtcacgcc tgaactcgaa gtatcaaaag
     2941 aaatattatc aaacaatata cgatgatcca ccctatacga aatattttct actttcattt
     3001 tttctccttt tgaactaata gcaaaagaaa cagttctgtt ttcatactct ttccccttca
     3061 tctatattat tcgtaatgaa aaataaaaaa aataaaattt ttttaaatga ttatgattca
     3121 gaatttattc aatatattaa aacaatattt gaacaaataa attgggagat tgaaattcat
     3181 ttctagcaat ttgttagaag tcacagtcta ctattctaac ctcagtatac tatatgtgtt
     3241 acaagaaaag actcaaaaac ttctcgtcag tgtaagaata tttgaaacac tatttaaaac
     3301 taaataacta taagaagtag atcaggtgaa caatttcagt acctactcta taagctatac
     3361 gaagatattt ttcaacatga tggataaaac gatcactctt ttaaagccat cttatttaga
     3421 aaagagaaac catttaagca tagatgggac cttcttgccc tgttttaaat ttttcttaaa
     3481 ctagagtctg taaaagattg gcttcatttt cacattttct cctaggagga atgtcgtaat
     3541 gcaaaagaat taaattcaaa taaatcatag cgatgacgac taatcgagta ctcaaacgga
     3601 gttccgttat ccaaataagc cacttgttct acttcaaata ccggttccgt tggaaggagc
     3661 tgcaagtaat gttgctcatt ttctgaacta gaagaagctc gtaagatttt tgtagcactg
     3721 gcaatatgca atcctaactt tccttcaatg taattgtaaa tcgatttttg taaaatatct
     3781 tcagtaatac ctggaataac atcggtactc atataagttt gttccagaac ataaggttta
     3841 ccgtcaataa tacgcaggcg gtaaatatta taaactggac tctgcaaagc gacctgtagt
     3901 ttttctgcta aaaattcatt tgcaaattct aatttgaagt gaataatcct cgagtcaatt
     3961 gtagaatgag catcttcaga tatttttgtc agtccccgga tatctctttc tggaacgatt
     4021 aattttcttt ttgagctgcc acgagagaga acaaaggttc cctgcccacg ttttctaaaa
     4081 attaagccct cagaaactaa caagtctaaa gcttttttga tcgtcattcg actacaggca
     4141 aactgtttag ctaatgtcat ttcatcaggt aattgttcgt tcatggtata tttattacaa
     4201 agaatctttt cacgaatctc attgtaaata aataaatatt ttgtactcat ttaatctggt
     4261 tctctctata atattctaat agtagtataa caaagttcaa gaaatgaggc aaacacaaga
     4321 tatgtatcaa caatttttaa aaatgtctag acaaatttta aaattatgct atactttatg
     4381 tatagaataa tagaaaggag gttgagcatg aaaaaaaagt tagtatttcc taatctgttt
     4441 tggtggggag cagcttctag cggacctcag acagaaggtc aatatggaaa agtacatgaa
     4501 aatgtgatgg actactggtt caaaacgcat ccagaagatt ttttcgataa tgtcggacct
     4561 cttgtagcca gtaacttttt tcatacttac accgaagatt tccacttgat gaaggaaatt
     4621 ggagttaatt ctttccgcac ttccatccaa tggagtcgac tcatcaagaa tttagagaca
     4681 ggtgagcctg atccaaaagg tattgctttc tacaatgcca tcattgaaga agctaaaaag
     4741 aaccagatgg atcttgtgat gaatttacat cattttgatt taccagtgga acttcttcaa
     4801 aaatacggtg gttgggaaag caaacatgta gtggagttat tcgtgaagtt tgccaagact
     4861 gctttcacat gctttggaga taaggttcat tactggacaa ctttcaatga gccaatggtc
     4921 attccagaag caggatactt atatgctttc cattatccaa atctaaaagg aaagggaaaa
     4981 gaggccgtac aagtcatcta taatctaaac cttgctagtg caaaagtgat tcaactatat
     5041 cgctcattag aacttgatgg aaagattggg attattttaa acttgacacc tgcttatcca
     5101 agaagtaatt ctccagaaga cttagaagca agtcgattta cagatgactt ctttaacaaa
     5161 gtcttcttga atccagctgt taaaggaact ttcccagaaa gattggtaaa acagctagag
     5221 agagatggcg tgttatggag tcataccgaa aaagagcttc aactgatgaa atcaaatacg
     5281 gttgattttc ttggagtaaa ctactaccat ccaaaacgtg ttcaagcaca agcaaatcct
     5341 gaggaatatc agacgccctg gatgccagac caatacttca aagagtatga atggccggag
     5401 cgtcgcatga atccatatcg tggttgggaa atttttccga aatccattta tgatattgct
     5461 atgattgtga aggaagaata tggtaatatc ccatggttta tcagtgaaaa cggaatgggt
     5521 gttgaaaacg aagcacggtt tatcgatgaa aatggagtta tcgatgacgt gtatcgtatt
     5581 gaattttatg aagaacattt aagatggcta cataaagcca ttgaagaggg aagtcactgt
     5641 tttggatacc acgcttggac cgcatttgat tgctggtctt ggaataatgc atataagaat
     5701 cgttacggat ttatctccgt tgatttagaa acgcaaaaga gaaccatcaa gagctcagga
     5761 agatggtatc gcaaagtaag tgacaataac ggttttgaag tagaaattga ggagtaaaga
     5821 gaggagatat tcatatgaaa aactcaaaat ttatagacca atttgccacc tttgctggta
     5881 aactagggaa ccaaattcat ttaaaaaccc taagagatgc attcgtaaca gtaatgccat
     5941 tatatatttt ggcaggttta atcgttcttt tgaacaacac ggtatttaag tggattttcc
     6001 aaggggatac attaacaaga ttccaatatt ggggaataac aattgcaaac ggtactttaa
     6061 gtatttcagg tatgattatt gctgtaatgg ttggttattt cttagctaaa aacagagatt
     6121 tcgaaaaccc gttagcagca tcaatgctat cattagtttc tttaattgtg atgatgccaa
     6181 atacagtttc tgtagttcct gacggagcaa aagatgctgt aaacatttca ggtgttcttt
     6241 cattcaacaa cacaggtaca ggcgcaatgt tcgccggggt tatcgtagcg attattgcaa
     6301 cagaattatt cattgaatta tcaaatgtta aagctttaca aatgaacctt ggtgaaaata
     6361 ttccaccagc tgttagtaga tcatttagcg tattacttcc agtcatgacc gtcatctcct
     6421 tatttggggt tgtttcagca ttattattca atataactgg aatgaactta atctcaatca
     6481 ttacaatctt tattcaagaa ccaattcgtc atattggtac aagcttaatc ggggtcatta
     6541 ttatttactc tttaggaaat atgttatggc tatttggtat tcaccaagca gttatttaca
     6601 gtgccatcct agaaccatta ctattaatta acattactga aaacatcact gcagcaaata
     6661 atggacaagc cattccacac atcatcaacc tatcacaaat acaaacattc gctttaatgg
     6721 gtggtagtgg atctacatta tgtttattaa tagcaacatt cttagtgagt cgcaatgctg
     6781 tctctaaaaa cgtggctaaa ttatcttttg gacctggtat cttcaatatc aatgaaccag
     6841 tattattcgg ttacccaatc gtttataaca tttcattagc tattccattt atcacagttc
     6901 cagtccttgg tattttaatc agctacttag caacagttac aggattcatg agtcctgcat
     6961 ttatacaagt tccttggact acaccagtat tcttaaatgc atggttagca acagcagggg
     7021 acgtgagagc agttctagtt caattcatca tctttgcact tggagttctt ctatacattc
     7081 catttatcaa agttaatgac aaagttgttg aacaagaaat ggaaggttaa gattatgaaa
     7141 caaattttat tagtatgtaa tgcgggaatg tcgacaagta tgcttgttaa aaaaatgcaa
     7201 caaagtgcga ctgaacgtgg gattgaaatc tctatccaag caaaatcaat gacagaagct
     7261 aaaaagaata tccatgaagc agatgtcatt ctaattgggc cacaaattcg ttatgagtta
     7321 cttgctgtga aagaaattgc aggaaatatc cctgtagata ccattgatat gcgggattac
     7381 ggaatgatga atggagcaaa agttctggaa caagcacttg catggatagg agaaataaga
     7441 tgacagaaga aatggaatat atctgttttc agctcattgc caatagtgga gcagccaaat
     7501 catcctttat tgaagccatc caactagcta aggcaggaaa tttaaaagaa gcgaaaatta
     7561 aagttgaaga agcggaagac tccttagtag aagcgcacaa gattcactct aatctcatac
     7621 aaaaagaagc tactggagaa aaaataggat tctccttgct atttatgcat gcagaagacc
     7681 aaatggcttc aacagagatt attcagttgc tatctaaaga attcatcgat ctctatcaaa
     7741 ataaatagga acactgtcat tcccatctgt ctttattaat gacgactaca ttgtaggaac
     7801 ctccaaaagt tggacaaaat gattgaaagg attggatcct gcattacaca agatccaatc
     7861 cttttctttt actctagttt ttataatatg ttgaatctag aatagtccac tgtagtttct
     7921 aaaaaattgc tggaaatgga tgttaagctc caatttattt gtttatatct tatttcagtt
     7981 tactatacta gcttattatt ctagtgaaaa attgaatgag ccactattta tttaatatgt
     8041 atgatgaggg aatgactcaa aaacttcttg tcgatataag aatatctgag atagtatttt
     8101 aaaacgaaag gactataaaa gctcatttcc ttcgcactta ttttatagag atctatcatt
     8161 atactcttta gaaatcaaaa agcaacacac cttgtcatcg ttttatcgta aatctgttaa
     8221 tgagggggct agtcttatct gcaatctcaa atcagtgttt tgagctgaat ggcactcgtt
     8281 ttctagtttg ctctttgatt ttaattgggc cttagtaaat actactctta aattaaacta
     8341 tagtgaattg aaataagatg tgaacaactc tatcag
//
LOCUS       DAVAAL010000078         8370 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVAAL010000078 DAVAAL010000000
VERSION     DAVAAL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.079"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            71..547
                     /locus_tag="VQM60_001432"
     CDS             71..547
                     /locus_tag="VQM60_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET1081295.1"
                     /translation="MPYFIGGHPGFNCPLLDDGVYEDYYLESEKEETCSVPRPFPETG
                     MLDFQDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDKGLKVHFAEFP
                     NLIIWSTLNKGPFITFEPWSGLSTFLEEGEHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            704..1129
                     /gene="lacA"
                     /locus_tag="VQM60_001433"
     CDS             704..1129
                     /gene="lacA"
                     /locus_tag="VQM60_001433"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET1081296.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFIVATKIKGMVAAEVSDERSAYMTRSHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1160..1675
                     /gene="lacB"
                     /locus_tag="VQM60_001434"
     CDS             1160..1675
                     /gene="lacB"
                     /locus_tag="VQM60_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET1081297.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHVETHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1686..2615
                     /locus_tag="VQM60_001435"
     CDS             1686..2615
                     /locus_tag="VQM60_001435"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET1081298.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGNLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            2617..3597
                     /gene="lacD"
                     /locus_tag="VQM60_001436"
     CDS             2617..3597
                     /gene="lacD"
                     /locus_tag="VQM60_001436"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET1081299.1"
                     /translation="MALTEQKRARLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3831..4667
                     /locus_tag="VQM60_001437"
     CDS             3831..4667
                     /locus_tag="VQM60_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET1081300.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGFKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4703..5020
                     /locus_tag="VQM60_001438"
     CDS             4703..5020
                     /locus_tag="VQM60_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET1081301.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5020..6711
                     /locus_tag="VQM60_001439"
     CDS             5020..6711
                     /locus_tag="VQM60_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET1081302.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARYFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FNLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALVFVQEQFD"
     gene            6926..8332
                     /gene="lacG"
                     /locus_tag="VQM60_001440"
     CDS             6926..8332
                     /gene="lacG"
                     /locus_tag="VQM60_001440"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET1081303.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDHENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLNL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLIDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
BASE COUNT         2626 a         1512 c         1745 g         2487 t
ORIGIN      
        1 tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta ataaagaaac
       61 tagcaaggtc atgccttact ttattggtgg acatccagga tttaattgtc ctctacttga
      121 tgatggagtc tatgaagatt attatctaga gtctgagaaa gaagagactt gctctgttcc
      181 acgtcctttc ccagaaacag gtatgttgga tttccaagat agaagtccat ggctagaggg
      241 acaaaaagaa atagatctta gttatgatct tttcagcaaa gatgcagtga ctttggatga
      301 attgcaatct cgaacaattg cccttcgttc tcttaaacat gataagggat tgaaagtgca
      361 ttttgcagaa tttccaaacc tcatcatctg gtcaactttg aacaagggac ctttcattac
      421 ctttgaacca tggtctggct tgtcaacatt ccttgaagaa ggagaacatt tagaagataa
      481 gaagaatgtt tgtctcttag aagccaatca ggttgaagaa ttagggtttg agatagaagt
      541 tttataaaaa agtaaaaaat aaataacaaa aacaaacaca aactattgac ttcttcaaac
      601 aatagtagta tattatgttt gtgaggacta attgactgtt ttttaaagac agccaataca
      661 ctgctggaat ccagcataga aaaaataaaa aggagtatac aatatgtcta ttgttatcgg
      721 tgcagatgct gcaggtttga gattgaaaga agttgtgaaa gacttcttag aaaaagaaaa
      781 cttccacctt gtggatgtta cagctgaagg tcaagacttt gttgatgtga ctctcgctgt
      841 tgctgcagaa gtaaacaaag aagaacaaaa ccttggtatc gtgattgatg cttatggagc
      901 tggtccattt atagttgcaa ctaagatcaa aggaatggtt gctgcagaag tatctgacga
      961 acgttcagct tatatgactc gtagccacaa caactcacgt atgatcacta tgggggcaca
     1021 acttgttggt gatgaattgg ctaaaaacat cgctaaagga tttgttaatg gtaaatacga
     1081 cggtggacgt catcaaatcc gggttgacat gttgaacaaa atgggctaat tagattacag
     1141 taagaaaggt aagttaaaaa tgagaattgc aattggatgc gaccacatcg taacagatga
     1201 aaaaatggcg gtttcagaat ttttgaaatc aaaaggatat gaagtcattg actttggtac
     1261 atatgaccat acacggactc actacccaat ctttggtaaa aaagtagggg aagctgtaac
     1321 tagcggccaa gctgatcttg gagtatgtat ctgtggtact ggtgttggta tcaacaacgc
     1381 tgtaaataaa gttccaggtg ttcgctctgc cttggttcgt gatatgacaa cagcccttta
     1441 tgctaaagaa caattgaacg ccaacgttat cggttttggt ggtaagatta ctggtgaatt
     1501 gctcatgtgc gatatcatcg aagctttcat ccatgctgaa tacaaaccat ctgaagaaaa
     1561 caaaaaattg attgcgaaaa ttgaacatgt tgaaacacac aatgctcaac aaacagatgc
     1621 aaacttcttc actgaattcc ttgaaaaatg ggatcgcgga gaataccacg actaagaggt
     1681 gacctatgat tttaacagtc acaatgaacc catccattga tatttcctat cccttggatg
     1741 agttgaagat tgatactgtc aatcgtgtgg tagatgtaac caaaacggct ggtggtaaag
     1801 ggctcaatgt tacacgcgta ctttcagaat ttggcgattc tgttcttgct actggtttag
     1861 taggtggcaa ccttggtgag tttttggttg aacatatcga taatcaagta aagaaagatt
     1921 tcttctcaat tcagggagaa actcgtaact gtatcgctat tctccacgga gacaaccaaa
     1981 cagaagttct tgaaaaaggt cctgaagtat tggaacagga aggtcaagac tttttggaac
     2041 atttcaaaaa actcttggag tcagttgaag tagtggccat ctcaggtagt ctgccagctg
     2101 gccttccagt tgattactat gcgagcttgg tagaacttgc taatcaagct ggcaagcctg
     2161 tagtcttgga ctgctcaggt gcagcacttc aggctgttct tgaatcaccc cataaaccaa
     2221 cagtcatcaa accaaataat gaagaattgt ctcagcttct tggaagagaa gtttctgagg
     2281 atttggatga attaaaagaa gtacttcaag aacctttgtt tgcagggatt gaatggatta
     2341 tcgtttcact tggtgccaac ggtacttttg ccaaacatgg tgacactttc tacaaggtag
     2401 atattcctag aattcaggtg gtaaatcctg ttggatctgg agactctact gtggcaggaa
     2461 tttcttcagg acttcttcac aaagaatcgg atgcagaatt actcatcaag gcaaatgtcc
     2521 ttggtatgct caatgctcaa gaaaaaatga ctggtcatgt caacatggcc aactatcaag
     2581 ttctatatga tcaattaata gtaaaagagg tataaaatgg ctttaacaga acaaaaacgt
     2641 gcacgcttag aaaaactttc tgatgaaaat ggtatcatct cagctcttgc atttgaccaa
     2701 cgtggtgctt tgaaacgcct catggctcaa caccaaacag aagaaccaac tgtggctcaa
     2761 atggaagaac tgaaagtctt ggtagcagat gaattgacta aatacgcttc atcaatgctt
     2821 cttgaccctg agtatggact tccagcaact aaagctcttg atgaaaaagc tggtcttctc
     2881 cttgcttatg aaaaaacagg ttatgacaca acaagtacaa aacgcttgcc agactgcttg
     2941 gatgtttggt ctgcaaaacg tattaaagaa gagggtgcag atgcagttaa attcttgctt
     3001 tactatgatg tagatagttc agacgaactc aaccaagaaa aacaagctta tatcgagcgt
     3061 atcggttctg agtgtgtggc tgaagatatc ccattcttcc ttgaaatcct tgcttacgat
     3121 gaaaaaattg cagacgcagg ttctgtagaa tatgcgaaag taaaaccaca caaagttatc
     3181 ggtgctatga aagtcttttc agacccacgc tttaacattg atgtcttgaa agttgaagtt
     3241 cctgttaaca ttaaatatgt tgaaggcttc gctgaaggtg aagtggttta cacacgtgaa
     3301 gaagcagcag ccttcttcaa agcgcaagat gaagcaacga acttgccata catttacttg
     3361 agtgctggtg tatcagctaa actcttccaa gatactcttg tatttgctca tgaatcaggt
     3421 gcaaacttta acggagttct ttgtggccgt gctacatggg caggatcagt tgaagcttac
     3481 atcaaagatg gtgaagcagc agctcgcgaa tggcttcgca caactggatt tgaaaacatt
     3541 gatgagctca ataaagttct tcaaacaaca gcgacttcat ggaaagaacg tgtgtaagaa
     3601 agtcctccta gtttaggaac atgaatctaa aaaaatttaa aaaaagttgt atgtaaaggt
     3661 ttacaaaata acttacttgt gctatactta aatcacaagt taatacaagg tgagtgttac
     3721 taagtaatat taggcatgat cacaggtgaa ttagaaatca gctgattttc tagttcattt
     3781 gtggtcattt tttgtactca tatactttta agatataaaa ggaggttgac atgtatcgaa
     3841 ttctaaatcc aatgaatcac aatgtctcgc ttgtcagaaa tgataaggga gaagaggtga
     3901 ttgtaattgg taaggggatt gcattcggaa agaagaaggg ggatttgatt gctgaaaatc
     3961 aggttgagaa aatctttcgg atgaagaccg aagagtccag agaaaacttt atggctcttc
     4021 tcaaagatgt tccgcttgat tttatcacag tgacctatga aatcattgat aagctatcaa
     4081 agaaatatca ttatccgatt caagagtatc tctatgtaac cttgacagat catatttact
     4141 gttcttatca agctctaact caaggaaggt acaaggatag taatctgcca gatatttccg
     4201 ctaagtatcc tgtcgctttt caaatcgcaa atgaagcttt tgaaatttac cgtcagaagc
     4261 tagcagatca ttttcctgag gacgaaatta ttcggattgc ttatcatttc attaatgctg
     4321 aaggtgaaaa tgaagtggaa cttgtggagt cgattgataa gaggaaagaa attctcagga
     4381 atgttgaaga agttttaacg gactatgcaa ttcaacgaac taaaaagaat aaccatttct
     4441 atgatcgctt tatgatccat ttgaattatt tcttggatta tttagacaga tctagagatg
     4501 ataaccaatc acttctggat atggaagatc atattaaaca atcctatcca aaagccttcg
     4561 agattggttt caagatctat gatgtgatta cgcaacatac gggtcttgat ttgtataaaa
     4621 gtgaacgagt ttatctagtt ctacatatcc aacgtttatt gtcataaaaa tttatttaaa
     4681 actatataag gagaattcta tcatgaatag agaagaagta acattgttag gttttgaaat
     4741 cgtagcctat gctggcgatg ctcgttcaaa actattggaa gccttgaagg ctgctgaagc
     4801 tggtgatttt gaaaaagcgg acgctctggt agaggaagct ggtagctgta ttgcagaggc
     4861 tcaccacgcg caaacaagtc tattgactaa ggaagcttca ggtgaggact tggcttatag
     4921 tgtaaccatg atgcatggcc aagaccactt aatgacaact atcttgttaa aagatttgat
     4981 gcatcattta attgaactct acaagagagg agttaaataa tgaataaact aattgcattt
     5041 atcgagaaag gaaagccttt ctttgaaaaa ctatctcgta atatctatct tcgtgctatt
     5101 cgtgatggtt tcattgcagg tatgcctgtt attctcttct caagtatctt tatcttgatt
     5161 gcctttgtac caaactcatg gggctttaaa tggtctgatg aagttgtagc ctttctgatg
     5221 aaaccttata gctattctat gggtattctg gctctcttgg tagctggtac aacagctaag
     5281 tcattgactg actcagtaaa ccggagcatg gaaaaaacca atcaaatcaa ctatatgtca
     5341 acattgttgg cagcaattgt tggtttgttg atgttggcag ctgatcctat cgaaagtggt
     5401 ctagctactg gattcttggg gacaaaaggt ttgctttcag ccttccttgc tgcctttgtt
     5461 actgtagcca tctataaggt ttgtgttaag aacaacgtca ctattcgtat gcctgacgaa
     5521 gttccaccaa atatctcaca agtctttaaa gatgtgattc cattcactct atctgttgtt
     5581 tctctttatg ctcttgactt attagcacgt tattttgttg gttctagtgt ggcagaatca
     5641 atcggtaaat tcttcgcacc actcttctca gcagcagacg gataccttgg tattaccatt
     5701 atctttggtg cctttgcctt cttctggttt gttgggattc atggtccatc tatcgttgaa
     5761 ccagctatcg cagctattac ctatgccaat gccgaagtca acttgaacct tctccaacaa
     5821 gggatgcatg cagacaagat tcttacttct ggtacacaaa tgtttatcgt taccatgggt
     5881 ggtacaggtg cgacattggt cgttccattt atgttcatgt ggttgacaaa atcgaaacgt
     5941 aaccgtgcaa tcggacgtgc ttcagtagtt cctaccttct tcggtgtaaa tgaaccaatc
     6001 ttgtttggtg cacctcttgt tttgaatcca atcttcttca ttccatttat ctttgctcca
     6061 attgcaaacg tatggatttt caaattcttt attgaaactc ttggaatgaa ctcattcact
     6121 gctaatctac catggacaac tccagctcca ctaggtctag ttcttggaac taacttccaa
     6181 gtgctatcat tcattcttgc tgcccttcta attgtggttg acgtagttat ttattaccca
     6241 ttccttaaag tctatgatga acaaattctt gaagaagaac gttcaggtaa gtctaatgat
     6301 gaattgaaag aaaaagttgc tgcaaacttc aacactgcaa aagcggatgc tattcttgaa
     6361 aaagcgggtg tcgatgcagc acaaaatacc atcactgaag aaacaaatgt cctcgttctc
     6421 tgtgctggtg gaggtacaag tggactcctt gcaaatgctt tgaataaggc agcagcagaa
     6481 tacaatgtcc ctgtgaaagc agcagcaggc ggctatggtg ctcaccgtga aatgttacca
     6541 gagtttaatc tagttatcct tgcccctcaa gttgcttcaa actttgaaga tatgaaggca
     6601 gaaacagata agctcggtat taaacttgct aagacagaag gcgctcaata catcaaatta
     6661 actcgtgatg gaaaaggtgc cctagtattc gtacaagagc aattcgatta aggctaggaa
     6721 ctgtgaaaca gtctcccatc gttacggaaa tcgctatggc gaatttccta atcgccttat
     6781 taattcgtcg gtaaaaagat atcgttttta cctcctcatg tcacaattca gtgacttggt
     6841 acaagaagtg agatggagaa gcatggctca ctgactcctc tcctctcact tttactttat
     6901 ttaaatcaag aaataggtga aaaaaatgac aaaaacactt ccaaaagact ttattttcgg
     6961 tggcgcaaca gctgcttatc aagcagaagg tgctacacat actgatggta aaggaccagt
     7021 agcgtgggat aaatatcttg aagataacta ctggtacact gcagaaccag ccagtgattt
     7081 ttacaatcga tatccagttg acctcaagct agcagaagag tatggtgtca atggcattcg
     7141 aatttctatt gcctggtcac gtattttccc gactggttat ggtcaagtaa atgctaaagg
     7201 tgttgagttt tatcataatc tatttgcgga gtgccacaaa cgtcatgttg agccctttgt
     7261 aactcttcat cactttgaca cgccagaagc tctccactca aatggcgact tcttaaaccg
     7321 tgaaaacatt gaacactttg tagactacgc ggctttctgt tttgaagaat ttccagaagt
     7381 aaactattgg acaaccttca atgaaattgg accaatcggt gatggtcaat atttagttgg
     7441 taaattccct ccaggaattc agtacgacct tgccaaagtc ttccaatcgc accacaatat
     7501 gatggtgtct catgcacgcg cggtaaaatt gtacaaagag aaaggctata aaggggaaat
     7561 tggtgttgtt cacgccctgc ctactaaata tcctctagat catgaaaatc cagcagatgt
     7621 tcgtgcagct gagttggaag atatcatcca caataaattc atcttagacg caacttatct
     7681 aggtcgctat tcagctgaaa ccatggaagg tgtcaaccat atcttatcag tcaatggtgg
     7741 tagtttgaat cttcgtgaag aagattttac agcattagaa gctgcaaaag acttgaatga
     7801 tttcctagga atcaactact atatgagtga ctggatggaa gcctttgatg gagaaactga
     7861 aattatccat aatggtaaag gtgaaaaagg aagctctaag tatcaaatca aaggtattgg
     7921 tcgtcgtgta gctcctgact atgtatcacg cacggattgg gattggatta tctaccctca
     7981 aggtttgtat gaccaaatca tgcgtgtgaa gaaagattat cctaactaca agaaaattta
     8041 tatcactgaa aatggtctcg gctataaaga tgagttcgtt gataacactg tttacgatga
     8101 tggtcgtatt gattacgtga aacaacactt ggagatcttg tctgatgcga ttgcagatgg
     8161 agctaatgta aaaggttact tcatttggtc attaatagat gtcttctcat ggtcaaacgg
     8221 ttatgagaaa cgttatggtc tcttctacgt agattttgaa actcaagaac gttatcctaa
     8281 gaaatcagct cactggtaca aaaaagtagc ggaaactcag attatagact agtagaatta
     8341 gtcattagat atagaatttt agtgagtcaa
//
LOCUS       DAVAAL010000079         8222 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVAAL010000079 DAVAAL010000000
VERSION     DAVAAL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.080"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..231
                     /locus_tag="VQM60_001441"
     CDS             <1..231
                     /locus_tag="VQM60_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET1081304.1"
                     /translation="PKPDNSKPQADDKKPSTTNNLSKDKQPSNQASTNEKATNKPKKS
                     LPSTGSISNLALEIAGLLTLAGATILAKKRMK"
     gene            complement(450..1430)
                     /gene="dusB"
                     /locus_tag="VQM60_001442"
     CDS             complement(450..1430)
                     /gene="dusB"
                     /locus_tag="VQM60_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET1081305.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKGPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
     gene            complement(1417..2289)
                     /gene="hslO"
                     /locus_tag="VQM60_001443"
     CDS             complement(1417..2289)
                     /gene="hslO"
                     /locus_tag="VQM60_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET1081306.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2454..3881
                     /locus_tag="VQM60_001444"
     CDS             2454..3881
                     /locus_tag="VQM60_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET1081307.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            4040..5548
                     /gene="glpK"
                     /locus_tag="VQM60_001445"
     CDS             4040..5548
                     /gene="glpK"
                     /locus_tag="VQM60_001445"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET1081308.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5590..7416
                     /gene="glpO"
                     /locus_tag="VQM60_001446"
     CDS             5590..7416
                     /gene="glpO"
                     /locus_tag="VQM60_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET1081309.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKRTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALAQNDLAELKN"
     gene            7486..8190
                     /locus_tag="VQM60_001447"
     CDS             7486..8190
                     /locus_tag="VQM60_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET1081310.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
BASE COUNT         2315 a         1639 c         1930 g         2338 t
ORIGIN      
        1 ccaaaaccag ataatagcaa gccacaagca gatgataaga agccatcaac tacaaataat
       61 ttaagcaagg acaagcaacc ttctaaccaa gcttcaacaa acgaaaaagc aacaaataaa
      121 ccgaagaagt cattgccatc aactggatct atttcaaatc tagcacttga aattgcaggt
      181 cttcttacct tggcgggggc aaccattctt gctaagaaaa gaatgaaata gtgattgatt
      241 cattcaaaaa tgtttgtaaa aagtattcga cttaaaagaa aagagtctgg gataaaaaga
      301 ttttgatttt aggaattctt tattataaat ttttaaaatc gatagattag acaaaaaagc
      361 gaacaagaca gaattctgag tgtcagataa ctgattttgt tcgcttttta tatttaatat
      421 tatacttttg tcctaggctc ttttttgcat tatacttttt ccaattgcaa gagagcttca
      481 atctctgcta gggtgctagc ttgcgaaatg gctccacgga gtttggcagc gccagatgtt
      541 ccacggagat agtgaggagc gaggccgcgg aattcacgaa ctgcgacgtt ttctcctttg
      601 aggttaatca atcgtttcaa gtgttcgtag gcgatcttca tcttgtcttc aaatgtcaaa
      661 tcaggtagga tttctcctgt ttcaaagtaa tggttgattt ggttgaagag gtaaggattt
      721 cccatggcag ctcggccaat catgactgcg tcagcaccaa cttcttcgat gcgttgcttg
      781 gcttcttgga cagtacggat atcaccgttg gcgatgaatg gaatcttggt tagagcttgg
      841 gcaaccttgt aaagggtctc aaggtccgcg tggccagtat acatttgttc acgggtacgg
      901 ccatgcatgg cgagggcaga aacacctgca gcctcagcag cgagggcatt ttctactgca
      961 agagatgggt ccgcccagcc ggtacgcatt ttgacagtaa gtgggatatc aaggacagac
     1021 tggaccttgt tgatgataga gtagatcttg tcaggaccct tgagccacat agcaccagct
     1081 tcgttcttca cgattttgtt gacagggcag cccatgttga tatcgacgat atcggtcttg
     1141 gtgttttctt ggatgaattc tgctgcgcgt gctaggctgt cttcatcgct accaaaaagt
     1201 tggatagaga cagggttttc gccctcatcg atatgaagca tgtgcagggt tttttcgttg
     1261 ttgtattgga ttcccttgtc agagaccatt tccattacaa cgagtccagc tccaagctct
     1321 tttgctatgg tacgaaaggc tgagttggtc acgccagcca taggcgctaa aacggtacga
     1381 ttgggaatct caatattgcc aatcataaaa ggtgtattaa gatttgtcac gaatgagttc
     1441 ctccaggtcc ttttcatcaa agttgtaagt agtttggcag aattgacaag tgatttctgc
     1501 accgtggtct tcctctttca tttcctgtaa gtctgagctt ggaaggctgg caagagcgtt
     1561 cataaagcgt tcatggctac agtcacattg gaaacggatt tcttcttcag aaagacgctt
     1621 gtaggcttcg tccccgtaga tagccttgag gagggcttcg atatggtcgt cgctttcgag
     1681 aagagtagag atagctggca tttcttggat gcgtttttca aagcgagcaa tctcttcttt
     1741 cttggctcct ggcaagactt gaactaggaa accacctgca accttgacct tgtcttcctc
     1801 gtccaaaagg acattgaggc cgaccgctga aggcgtttgt tggctttcag taaggtaaaa
     1861 ggcaaggtct tcaccgattt ctccagagat gaggggagtt atagagttgt aaggatttcc
     1921 agtaccgtag tctgtgataa cgaggaattg accatttcca acaaaaggtc cgactaggac
     1981 ttcaccagtc gcagtctttt tgatgtcaac accaggattt tgaacatagc ctttgacgtt
     2041 ccccttggta tcagcgacgg tgataatagc acctagagag ctagatccca acaccttaac
     2101 tgtaagtttg gtatttcctt tttcattggc tgcgagaatc tggctagcga taagagttcg
     2161 accaagcgct acagttgagc tagcttgggt ttgatgtttt tcttgagcag tgcggacggt
     2221 ttcagtgcta tcaaggacaa aagcacgaaa ggctccgctt tctgatatag ttttaataat
     2281 tttatccata gctactattt tagcataaaa atgcccaaag ggggagccgt gtgtttactg
     2341 attttcagga taatggacca ggaaatcagc atgaaaataa aaagagaaac agattatttt
     2401 agcatttgtc agatttatgc tatgcttaag gtagaaaatg aaaggggtaa caaatgtatt
     2461 taggagattt gatggagaaa gccgagtgtg gtcaattttc aatcctttcc tttctattac
     2521 aagagtctca gacgaccgtc aaggctgtaa tggaagaaac aggattttca aaagcaaccc
     2581 taaccaaata tgtcaccctg ctcaatgaca aggctttgga tagtggctta gagctgacta
     2641 ttcactcaga agatgaaaat ctgcgtctgt ctatcggtgc agctaccaag gggagagata
     2701 ttcggagctt gtttttggag agtgctgtta aataccagat tttggtttat cttctctacc
     2761 accaacagtt tttagcccat cagctggctc aagaattggt gattagcgag gctacgcttg
     2821 gtcgtcactt ggctggttta aatcagattt tgtcagaatt tgatttatcc atccaaaatg
     2881 gacgttggcg aggtccagag catcagattc gctatttcta tttctgtctt ttccgaaagg
     2941 tctggtcgag tcaggaatgg gaaggtcaca tgcagaaacc agagagaaaa caggagattg
     3001 ccaatttaga ggaaatctgc ggtgcaagtt tgtctgcggg gcagaaattg gacttggttc
     3061 tctgggctca catcagtcaa caacgtcttc gggtcaatgc ttgtcagttt caagtcatag
     3121 aagagaaaat gcgagggtat tttgacaata tcttttatct tcgtttgctg agaaaggttc
     3181 cgtccttttt tgctgggcaa catattccac taggagttga ggatggtgag atgatgatat
     3241 tcttctcttt tctcctatct catcgcattc ttcctcttca tactatggag tatattcttg
     3301 gttttggagg gcagttggca gatttactga cgcaattgat tcaagaaatg aagaaggagg
     3361 aactattggg ggattataca gaggaccatg tcacctatga actcagtcag ctttgtgctc
     3421 aagtctatct ctataagggc tatattttac aggatcgcta caagtaccag ttagagaatc
     3481 gtcatccata tttactgatg gaacatgatt ttaaagagac agcagaggag atttttcatg
     3541 ctctgcctgc ttttcaacag gggacagatt tagataagaa gattctctgg gaatggctcc
     3601 agttaatcga atatatggct gaaaacggtg gccagcatat gcggattggt ctggatttga
     3661 catctggttt tcttgtcttt tcaaggatgg cagccatttt gaaacggtat ttggaataca
     3721 atcgttttat taccattgaa gcttatgacc ctagtcggca ttatgatttg ctggttacca
     3781 ataacccgat tcataagaag gaacagacac cagtctatta tttaaaaaac gacttggata
     3841 tggaagattt ggcagggatt cgtcagttat tattcactta aaaggcttgg ttaatccagg
     3901 tcttttttgt gaaattcaca caatctcctc acattttttt aaaaattaaa aaaagttgat
     3961 aaacaagaaa gcgctttatt ttgtatacta gtaagtgtaa agaggaaaca cctcaagatc
     4021 tttatcagga ggacagtata tgtcacaaga aaaatacatc atggccattg accagggaac
     4081 tacaagttct cgtgccatca ttttcaacaa aaaaggggaa aaggttagct cgagtcaaaa
     4141 agagtttacc cagattttcc ctcaggcagg ttgggttgag cacaatgcca atgaaatttg
     4201 gaactctgtt cagtcagtta ttgcgggtgc tttcatcgaa agtggtgtca agccaaatca
     4261 aatcgaggca atcgggatta ccaaccaacg tgaaacaacg gttgtctggg ataagaaaac
     4321 aggacttcct atctacaatg ctatcgtttg gcagtcacgc cagacagcac ctttggctga
     4381 gcaactaaaa agccaaggtt atgtggaaaa attccatgaa aagactggtt tgattattga
     4441 tgcttacttc tctgctacca aggttcgttg gattttggat catgtagaag gtgctcaaga
     4501 gcgagcagaa aaaggggaat tgctctttgg tactatcgat acttggttgg tttggaaatt
     4561 gactgacggt gcggctcacg tgactgacta ctcaaatgca gctcgtacca tgctttataa
     4621 cattaaagaa ctcaaatggg atgatgagat tttggaaatc cttaacattc cgaaggctat
     4681 acttccagaa gttcgttcta actccgaaat ctacggcaag acagctccat tccatttcta
     4741 cggtggagag gtgccaatct caggtatggc tggggaccaa caagcagccc tctttggaca
     4801 gttggctttt gagccaggta tggttaagaa tacttatgga acaggctctt tcatcatcat
     4861 gaatactggg gaagagatgc agttgtctga aaacaacctc ttgacaacca ttggttacgg
     4921 aatcaacggt aaggtttatt atgccttgga aggttctatc ttcatcgcag gaagtgctat
     4981 tcagtggctt cgtgacggtc ttcgcatggt tgaaaattca ccagaatctg aaaaatacgc
     5041 tcgtgattct cacaacaacg atgaagttta tgtcgttcca gcctttacag gtctaggcgc
     5101 tccatactgg aaccaaaatg ctcgtggttc cgtctttggt ttgactcgtg gaacaagcaa
     5161 agaagacttt atcaaggcga ctttgcaatc tattgcttat caagtgcgtg atatcatcga
     5221 caccatgcaa gtggatactc agaccgccat tcaagtactg aaggtggatg gtggtgcagc
     5281 catgaacaac ttcctcatgc agttccaggc ggatatttta ggcattgaca ttgcacgtgc
     5341 taaaaacctg gaaacaacag ctctaggagc ggccttccta gcaggtttgt cagtagggta
     5401 ctggaaagac ttggacgagt tgaaactctt gaacgagaca ggagaactct ttgagccatc
     5461 tatgaacgaa tctcgcaagg aacaactcta caagggctgg aagaaggctg tgaaagcaac
     5521 tcaagtcttt gcggaagtag acgactaata ctggcagaat aaagcgattt atttagaaag
     5581 tgtgtaaata tggaattttc aaagaaaaca cgtgaattgt caattaaaaa aatgcaggaa
     5641 cgcaccctgg acctcttgat tatcggtgga ggaatcacag gagctggtgt agccttgcag
     5701 gcggcagcta gcggtcttga gactggtttg attgaaatgc aagactttgc agaaggaaca
     5761 tctagtcgtt caacaaaatt ggttcacgga ggtcttcgct acctcaaaca attcgacgta
     5821 gaagtggtct cagatacggt ttctgaacgt gcagtggttc aacaaatcgc tccacacatt
     5881 ccaaaaccag atccaatgct cttaccagtt tacgatgaag atggagcaac ctttagcctc
     5941 ttccgtctta aagtagccat ggacttgtac gacctcttgg caggtgttag caacacacca
     6001 actgcgaaca aggttttgag caaggatcaa gtcttggaac gccagccaaa cttgaagaag
     6061 gaaggcttgg taggaggtgg agtgtatctt gacttccgta acaacgatgc gcgtctcgtg
     6121 attgaaaaca tcaaacgtgc caaccaagac ggtgccctca ttgccaacca cgtgaaggca
     6181 gaaggcttcc tctttgacga aagtggcaag attacaggtg ttgtagctcg tgatctcttg
     6241 acagaccaag tatttgaaat caaggcccgt ctggttatta atacaacagg tccttggagt
     6301 gataaagtac gtaatttgtc taataagaga acgcaattct cacaaatgcg cccaactaag
     6361 ggagttcact tggtagtgga ttcaagcaaa atcaaggttt cacagccagt ttactttgac
     6421 acaggtttgg gtgacggtcg tatggtcttt gttctcccac gtgaaaacaa gacttacttt
     6481 ggtacaactg atacagacta cacaggtgat ttggagcatc caaaagtaac tcaagaagat
     6541 gtagattatc tacttggcat tgtcaacaac cgcttcccag aatccaacat caccattgat
     6601 gatatcgaaa gcagctgggc aggtcttcgt ccattgattg cagggaacag tgcctctgac
     6661 tataatggtg gaaataacgg taccatcagt gatgaaagct ttgacaactt gattgcgact
     6721 gttgaatctt atctctccaa agaaaaaaca cgtgaagatg ttgagtctgc tgtcagcaag
     6781 cttgaaagta gcacatctga gaaacatttg gatccatctg cagtttctcg tgggtctagc
     6841 ttggaccgtg atgacaatgg tctcttgact cttgctggtg gtaaaatcac agactaccgt
     6901 aagatggctg aaggagctat ggagcgcgtg gttgacatcc tcaaagcaga atttgaccgt
     6961 agctttaaat tgatcaattc taaaacttac cctgtttcag gtggagaatt gaacccagca
     7021 aatgtggatt cagaaatcga agcctttgcg caacttggag tatcacgtgg tttggatagc
     7081 aaggaagctc actatctggc aaatctttac ggttcaaatg caccgaaagt ctttgcactt
     7141 gctcacagct tggaacaagc gccaggactc agcttggcag atactttgtc ccttcactat
     7201 gcaatgcgca acgagttggc tcttagccca gttgacttcc ttcttcgtcg taccaaccat
     7261 atgctcttta tgcgtgatag cttggatagc atcgttgagc cagttttgga tgaaatggga
     7321 cgattctatg actggacaga agaagaaaaa gcaacttacc gtgctgatgt cgaagcagct
     7381 ctcgctcaaa acgacttagc agaattaaaa aattaagaaa aaataaaaga ggtggagggc
     7441 agcattcctt gtcgcccgtc ccttcttttt aatggagaca gaaagatgat gaatgaatta
     7501 tttggagaat ttctagggac tttaatcctg attcttctag gaaatggtgt tgttgcaggt
     7561 gtggttcttc ctaaaaccaa gagcaatagc tcaggttgga ttgtgattac tatgggttgg
     7621 gggattgcag ttgcggttgc agtctttgta tctggcaagc tcagtccagc tcatttaaac
     7681 ccagctgtga ccatcggtgt ggccttaaaa ggtggtttgc cttgggcttc cgttttgcct
     7741 tatatcttag cccagttcgc aggggccatg ctgggtcaga ttttggtttg gttgcaattc
     7801 aaacctcact atgaggcaga agaaaatgca ggcaatatcc tggcaacctt cagtactgga
     7861 ccagccatca aggatactgt atcaaacttg attagcgaaa tccttggaac ctttgttttg
     7921 gtgttgacaa tctttgcttt gggtctttac gattttcagg caggtatcgg aacctttgca
     7981 gtgggaactt tgattgtcgg tatcggtcta tcactaggtg ggacaacagg ttatgccttg
     8041 aacccagctc gtgaccttgg acctcgtatc atgcacagca tcttgccaat tccaaacaag
     8101 ggagacggag actggtctta cgcttggatt cctgttgtag gccctgttat cggagcagcc
     8161 ttggcagtgc ttgtattctc acttttctag tttatactct tcgaaaatca aattcaaacc
     8221 ac
//
LOCUS       DAVAAL010000080         8216 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVAAL010000080 DAVAAL010000000
VERSION     DAVAAL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.081"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            150..830
                     /gene="radC"
                     /locus_tag="VQM60_001448"
     CDS             150..830
                     /gene="radC"
                     /locus_tag="VQM60_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET1081311.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(827..1516)
                     /locus_tag="VQM60_001449"
     CDS             complement(827..1516)
                     /locus_tag="VQM60_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET1081312.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(1534..2175)
                     /locus_tag="VQM60_001450"
     CDS             complement(1534..2175)
                     /locus_tag="VQM60_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET1081313.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(2258..2473)
                     /locus_tag="VQM60_001451"
     CDS             complement(2258..2473)
                     /locus_tag="VQM60_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET1081314.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(2481..2828)
                     /locus_tag="VQM60_001452"
     CDS             complement(2481..2828)
                     /locus_tag="VQM60_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET1081315.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(2833..3948)
                     /locus_tag="VQM60_001453"
     CDS             complement(2833..3948)
                     /locus_tag="VQM60_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET1081316.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(3958..4917)
                     /locus_tag="VQM60_001454"
     CDS             complement(3958..4917)
                     /locus_tag="VQM60_001454"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET1081317.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(5030..5599)
                     /locus_tag="VQM60_001455"
     CDS             complement(5030..5599)
                     /locus_tag="VQM60_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET1081318.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            5727..6398
                     /locus_tag="VQM60_001456"
     CDS             5727..6398
                     /locus_tag="VQM60_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET1081319.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            6382..7200
                     /locus_tag="VQM60_001457"
     CDS             6382..7200
                     /locus_tag="VQM60_001457"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET1081320.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            7197..8093
                     /locus_tag="VQM60_001458"
     CDS             7197..8093
                     /locus_tag="VQM60_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET1081321.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     DIRLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLDDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            8137..>8216
                     /locus_tag="VQM60_001459"
     CDS             8137..>8216
                     /locus_tag="VQM60_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransacetylase"
                     /protein_id="HET1081322.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPR"
BASE COUNT         2393 a         1566 c         1749 g         2508 t
ORIGIN      
        1 tggaagtttc aggtagcggt gctaggcagg aattgaaaat caatttccca gaagtaggtt
       61 tgaaaaaact tttagccagt gtggctccaa ttgagaaaaa aatctaattt tccatccttc
      121 tcacgaataa taaagtgagg aggattttta tgtacagtat ttcattccaa gaagattcac
      181 tattaccaag agaaaggctg gccaaggaag gagttgaagc gcttagtaac caagagttgc
      241 tagctatttt actcaggaca ggaacacgtc aagctagcgt ttttgaaatt gcccaaaaag
      301 tcttgaacaa tctttcaagc ctaacggatt tgaaaaaaat gaccctgcag gaattgcaga
      361 gtttgtctgg tattgggcgt gttaaggcca tagaattaca agctatgatt gaactggggc
      421 atcgtattca caaacacgag actcttgaaa tggaaagtat tctcagcagt caaaagttgg
      481 ccaagaagat gcagcaggaa ttaggggata aaaaacaaga gcacctggtg gcactctatc
      541 tcaatactca aaatcaaatc atccatcagc agaccatttt tatcggttct gtaactcgta
      601 gtatcgctga accgcgagag attctgcact atgcaatcaa gcatatggcg acttctctta
      661 tcttggtcca caatcatcct tcaggagcgg tagcgcctag ccaaaatgat gatcatgtca
      721 ctaaacttgt taaagaagcc tgcgaactga tggggattgt tctcttggac catttgattg
      781 tctctcattc taattacttt agttatcgtg aaaagacaga tttaatctaa agttcattaa
      841 cgacatagtc aaagagtttc ttatctttgg gacgattttc aaaaagaagt tctggatgcc
      901 attggacacc gagaaaggcg acatcatccg tactcatgac agcctcaatg ataccatctt
      961 taggatcatg agccacaact tttaaatttg gtgctaagtc cttgatgctc tggtggtgga
     1021 aggagttgat atgagagatt tctccataga tttcttggag aacggtatct ggttctgtta
     1081 ccaagcgttg agttgtgtac tcaacagaag aatcctgcca atggtcttcg atatcttggt
     1141 acaaagttcc acccatggca acgttaaaga gttgggtacc acggcagaca gagaaaatgg
     1201 gctttttctg tttaatagct tccttgatga gggccagttc gaagatatct ctttgaaggt
     1261 gataatcatc gctatcaatg gttttgggtt cgccatagaa ttttggatcg acattttgcc
     1321 cacctgtcaa gatgagcttg tcaatcaaac tgatatagtg gcaggccatt tcttgatcac
     1381 caatcggtag gatgatggga atccctccag catctttaac gccttcaaca aagccttttg
     1441 ctgcgtagct catcatgatg tcatcatctg gatgagtttt ttcgtttcct gtaatcccaa
     1501 taactggttt tttcataaaa tgattttcgc tttctaatcc tcttttcgca tgaagtagag
     1561 gagggtttgg agttcacttg tcaaatcgac atactgaacg accacgtctt ttggtaaatg
     1621 cagatggact ggtgaaaaac tgagaattcc tttcacacca gcatcaacca agagattagc
     1681 aacctcttgt gacttgacgc tgggaacagt taggatagca gtcttcacat cagcatcctt
     1741 gattttatcc ttgatctgag aaatcccgta aatgggaatc ccgtcaggag tttgggtacc
     1801 gacttcagga tggtcgtcta ggtcaaaggc catgataatc ttcatcttgt tacgttcgtg
     1861 gaagcggtag tggagaaggg catggcccat atttccaata ccaaccagca tgacattggt
     1921 aatagagttg tcattgagca aatcggcaaa aaatgtcatt agttttttga catcatagcc
     1981 aaaaccacga cgaccaagtt caccaaaata ggaaaaatca cgacgtacgg tcgctgaatc
     2041 aataccgata gcctctgcaa tttgcttaga gttggcacgt tcaatctttt ctgcatgaaa
     2101 tctcttaaaa attcgatagt agagagagag tctttttgct gtagcttttg gaatagcaaa
     2161 ctgtttatct ttcacaaaat cacaaccttt ctattcttct attttataga aacattgtga
     2221 aaaaatcaac aaaaataaga aaaaactaag aaaaatctta gttttgatgt aaaaaatctg
     2281 catgagatag aaaacggtag aggtctccga ccagcccctg ataaactttt ttgcccctaa
     2341 aagtcagaga agtcacataa agtgtatctg gtaaggttac acatcctgac aaagtcaaca
     2401 tgagagcctc atgatcctca tacttgagag tacgctctac atgatagcag tccttatagg
     2461 tcagttcaaa cattttggct ctatctttcc gattttgtaa agacaccacg ttctaccaag
     2521 ctatccatga ggaagtagaa tttttcctga tgaatatggt ggtcttctga tttgaaaata
     2581 tcaactagac gaaggccaaa cttgtcagtg atattgattt tagcccctgt aagttccttg
     2641 ttaatgatga ttttgagttg gaagccttca ccgctgtttg gcactttttc caaaaggcga
     2701 gtcagttcat agttaccaac cttagtttca aaaaaggtgt tatctttgag ggtgaatttt
     2761 ttaacagaag ggctaagagt gtaatcgtaa cgacaatttt ttaactgaat gattttttca
     2821 aatgccatat ggctaacctc cgataatttc ttttaaggtt tttgcgaggg tttgtaggtc
     2881 ttcaacggta ttttgtggcg acaaactgat gcgaagggat tccttcaagc gttctgaatt
     2941 tgcgccatac atggctttaa gaacatggct ggattggaca acgcctgcag tacaggctga
     3001 gccagtagag attgaaattc cagctaaatc tagccgaagg agtaagaggt catttttctg
     3061 accaggaaat ccaatattga gaacataagg gagatgatgt tttcctctat tcaagtaata
     3121 ctgaatgccc tccagctctg ccagaaaggc agtttctaga ttttgtacat gttgaaaatg
     3181 ttcttcttgt ttttctaggt cttcttttag ggctgcaacc atgcctacga tggcaggcag
     3241 attttcagtt cctgcacgtt ttttctgttc ctgatctccg ccatgtagat aggaatcaaa
     3301 gtccatgcta gatgcgtaga gaaaaccgat tcccttagga ccatggaatt tgtgggcaga
     3361 agcagtgaga aaatcaatgc ccaattcttc tgaatgaatt gggattttac caatagcctg
     3421 aactgcatca acatgatagg cagcagggtg ttgcttgagt atttggccaa tttcagcgat
     3481 gggaagtagg tttcctgtct cattattggc aaacatagta gaaaccaaaa tcgtatcgtc
     3541 acgtaaagcc ttttgaattt gctgggctgt gatttcttga ttttctggct ggataatggt
     3601 tgcttcaaac ccaaagtgtt gaaccaagta atcaattgtt tcaaggacag catggtgctc
     3661 gatggcagtt gtgatgatat gttttccttg ttcttggtga cgaagacagt agccaatgat
     3721 ggtagtatta ttgccttcag tcccaccaga agtgaaaaag atatgttgag gttttgtcct
     3781 tagtaactgg gctagttcct gacgggcttc tcgcaagagt ttgccagctt gacgaccatg
     3841 accatgaata ctagaaggat ttccgtgggt ttcttgcata accttggtca tagctgaaat
     3901 agcaactgct gacataggag tcgttgcagc attgtccaaa taaatcaaag aatcacctta
     3961 tttcttttta ttgtaggcaa agagtgggct gactggtttt ctttcgtgaa tacggacgat
     4021 agcatcacca attaactcac tagcagtgat gtagcataca tttttaggag ttttttcttt
     4081 tgttgctact gaatcagtca caagaatttc tttaatatta gtattgtcaa gaagctcagc
     4141 agctccctcg acgaagagac cgtggctaga aacagcataa atttctgtag ctccttcacg
     4201 ttcaacgatt ttagaagctt cagagaaggt acgtcctgta tttaaaatat catcaatcaa
     4261 gatagctttc ttaccttcaa catcaccaat aatataacct tcgttacgag ttgcatcgtc
     4321 ttgagggtag tcgataatgg cgataggagc atcaagatat tcagccaggc tacgcgcacg
     4381 tttgacacct gaatttttag ggctaacgac aacaacatct gaaccaagca atcctttatc
     4441 gcagtaatgt tttgcgaata ggggaacagt gaaaagatta tccactggaa tatcaaagaa
     4501 accttgaacc tgaacggcat gcaaatcaag agtcaggata cgatcaactc cagccttaac
     4561 cagcatattg gcaactagtt ttgctgtaag tggctcacga ggacaagcaa tgcggtcttg
     4621 acgtgcatag ccaaaatatg gaaggacaac gttgatactg tgggcacttg cacgcacaca
     4681 agcatcgacc atgattaaca attccattag gtggttgttg acagggaaac ttgttgattg
     4741 gatgatgtaa acatcataac cacggacact ttcttcgata tttacttgga tttctccgtc
     4801 tgaaaattga cgtgatgata gttttccaag tgggacacca acagcttggg caattttttg
     4861 tgcaatctct tggttagagt tgagtgcgaa aagtttcatg ttttttctat ctgacattat
     4921 agaccgtcct ctgtaaactt tataaatcct agttatattt accttacata tatgaactgg
     4981 gatttgtgta tttttatctt ttctatttta ccaaaaaatg gagattattt cagctatttt
     5041 tcatactttt gacaaatcga accaattttg aaggagcttt ttggtaggaa atctgatttt
     5101 tctctaaaaa ttgtcggaaa tcctgtttgc cttgctcatg attttccact tcaagctcca
     5161 attcgtaatc tgtcatatca aagtatcggc tctgatccag tgccatgaga ccaatagctg
     5221 ttttcatttc atagcgaagc gttgttagac aaccaagaac ctgccagttc ttactttgga
     5281 taccatgttt cgccaattca tccagtacta gcccttgagg aagttcttcc ttactcagat
     5341 agttctcagc atcttttagt tgcaattttt ggttgtattc catgtttcca acactctgcg
     5401 ggactttgag tgtcaactca gcccagtctt caaaggttcg aatgcgcata gcgactttct
     5461 tttctcgcag ttcaaaatca ggcgtgtcga tgtagtaatt tgtttgaaga acaggagtga
     5521 cacctgtgaa ctggtctttt agacgattgt attcatcttt tttcaatagt gttttcaatt
     5581 caatttctaa atgtttcatt tttcttacct ttttttatcg ttgaaagcgg atttatggta
     5641 taataagcat tgtatttatt gtatatgaat ctggagaaaa aatcaaagat atttttgacg
     5701 gataatatga gaacaaggga gaatatatga ccttagaatg ggaagaattt ctagatcctt
     5761 acattcaagc tgttggtgag ttaaagatta aacttcgtgg tattcgtaag caatatcgta
     5821 agcaaaataa gcattctcca attgagtttg tgaccggtcg agtcaagcca attgagagca
     5881 tcaaagaaaa aatggctcgt cgtggcatta cttatgcgac cttggaacac gatttgcagg
     5941 atattgctgg cttacgtgtg atggttcagt ttgtagatga cgtcaaggaa gtagtggata
     6001 ttttgcacaa gcgtcaggat atgcgaatca tacaggagcg agattatatt actcatagaa
     6061 aagcatcagg ctatcgttcc tatcatgtgg tagtagaata tacggttgat accatcaatg
     6121 gagctaagac tattttggca gaaattcaaa ttcgtacttt ggccatgaat ttctgggcaa
     6181 cgatagaaca ttctctcaac tacaagtacc aaggggattt cccagatgag attaagaagc
     6241 gactggaaat tacagctaga atcgcccatc agttggatga agaaatgggt gaaattcgtg
     6301 atgatatcca agaagcccag gcactttttg atcctttgag tagaaaatta aatgacggtg
     6361 taggaaacag tgacgataca gatgaagaat acaggtaaac gaattgatct gatagccaat
     6421 agaaaaccgc agagtcaaag ggttttgtat gaattgcgag atcgtttgaa gagaaatcag
     6481 tttatactca atgataccaa tccggatatt gtcatttcca ttggcgggga tggtatgctc
     6541 ttgtcggcct ttcataagta cgaaaatcag cttgacaagg tccgctttat cggtcttcat
     6601 actggacatt tgggcttcta tacagattat cgtgattttg agttggacaa gctagtgact
     6661 aatttgcagc tagatactgg ggcaagggtt tcttaccctg ttctgaatgt gaaggtcttt
     6721 cttgaaaatg gtgaagttaa gattttcaga gcactcaacg aagccagcat ccgcaggtct
     6781 gatcgaacca tggtggcaga tattgtaata aatggtgttc cctttgaacg ttttcgtgga
     6841 gacgggctaa cagtttcgac accgactggt agtactgcct ataacaagtc tcttggcggt
     6901 gctgttttac accctaccat tgaagctttg caattaacgg aaattgccag ccttaataat
     6961 cgtgtttatc gaacactggg ctcttccatt attgtgccta agaaggataa gattgaactt
     7021 attccaacaa gaaacgatta tcatactatt tcggttgaca atagcgttta ttctttccgt
     7081 aatattgagc gtattgagta tcaaatcgac catcataaga ttcactttgt cgcgactcct
     7141 agccatacca gtttctggaa ccgtgttaag gatgccttta tcggtgaggt ggatgaatga
     7201 ggtttgaatt tatcgcagat gaacatgtca aggttaagac ctttttaaaa aagcacgagg
     7261 tttctaaggg attgctggcc aagattaagt ttcgaggtgg agctattctg gtcaataatc
     7321 aaccgcaaaa tgcaacgtat ctattggacg ttggagacta cgttaccatt gacattcccg
     7381 ctgagaaagg ctttgaaacc ttggaggcta ttgagcttcc attagatatt ctctatgagg
     7441 atgaccactt tctagtcttg aataaaccct atggagtggc ttctattcct agtgtcaatc
     7501 actctaatac cattgccaat tttatcaagg gttactatgt caagcaaaat tatgaaaatc
     7561 agcaggttca cattgttacc agactagata gggacacttc tggcttgatg ctctttgcca
     7621 agcacggtta tgcccatgca cgattagaca agcagttgca gaagaaatct atcgagaaac
     7681 gctactttgc tttggttaag ggagatggac atttggagcc agaaggggaa attattgctc
     7741 cgattgcgcg tgatgaagac tccattatta ccagacgggt ggctaaaggt ggaaaatatg
     7801 cccatacttc ctacaagatt gtagcttctt atggaaatat ccacttggtc gacattcggc
     7861 tgcacactgg tcgaacccac caaattcgag tccacttttc tcatattggt tttcctttgt
     7921 tgggcgatga tttgtacggt ggtagtctgg acgacggcat ccaacgtcag gccctgcatt
     7981 gccattacct atccttttat catccatttt tagagcaaga tttgcagtta gaaagtccct
     8041 tgccggatga tttcagcaac cttattaccc agttatcaac taatactcta taaaaactgt
     8101 ttcagagtat aattattatc ttaaaggaga aaactcatgg aagtttttga aagtctcaaa
     8161 gccaaccttg ttggtaaaaa tgctcgtatc gttctccctg aaggggaaga gccacg
//
LOCUS       DAVAAL010000081         8156 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVAAL010000081 DAVAAL010000000
VERSION     DAVAAL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.082"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..812)
                     /locus_tag="VQM60_001460"
     CDS             complement(<1..812)
                     /locus_tag="VQM60_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET1081323.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLT"
     gene            complement(1292..1816)
                     /gene="grpE"
                     /locus_tag="VQM60_001461"
     CDS             complement(1292..1816)
                     /gene="grpE"
                     /locus_tag="VQM60_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET1081324.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(1843..2877)
                     /gene="hrcA"
                     /locus_tag="VQM60_001462"
     CDS             complement(1843..2877)
                     /gene="hrcA"
                     /locus_tag="VQM60_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET1081325.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREEQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(3041..3550)
                     /locus_tag="VQM60_001463"
     CDS             complement(3041..3550)
                     /locus_tag="VQM60_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET1081326.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            4158..6491
                     /locus_tag="VQM60_001464"
     CDS             4158..6491
                     /locus_tag="VQM60_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET1081327.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQSNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            6504..7967
                     /locus_tag="VQM60_001465"
     CDS             6504..7967
                     /locus_tag="VQM60_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET1081328.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILDPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            7967..>8156
                     /locus_tag="VQM60_001466"
     CDS             7967..>8156
                     /locus_tag="VQM60_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HET1081329.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT         2609 a         1495 c         1594 g         2458 t
ORIGIN      
        1 gtcaaagtca tttccaagtg aagaggtcca gcctcacctg cagtgataaa tggcaagctg
       61 atttgtgttg aagttacacc agaaaggtct ttcttcgctt tttcagccgc atctttcaaa
      121 cgttgcattg ccatcttgtc agtagacaag tcgataccgt tttctttctt gaattctgct
      181 accaagtggt caatgatttt ttggtcaaag tcgtcaccac caagtttgtt gtcccctgca
      241 gttgacaata cgtcgaagac accgtcaccc aattcaagga tagagacgtc gaatgtacca
      301 ccaccaaggt caaataccaa gattttttct tctttgtcag tcttgtccaa accataagca
      361 agagctgctg cagttggttc gttaacaata cgttctactt caagaccagc aattttacca
      421 gcgtcttttg ttgcttgacg ttgagcgtcg ttgaagtaag ccggaactgt gataacagct
      481 ttggttactt tctcaccaag gtagtcttca gcgtagcctt tcaagtattg aaggatcata
      541 gctgagattt cttgtggagt gtattctttt ccatttgcag aaactttttc agaagttccc
      601 atcttagatt tgatagagat aactgtatct gggtttgtga ctgcttgacg ttttgcagca
      661 tcaccaacga taatttctcc gtttttgaat gagactacag atggagttgt gcggtttcct
      721 tctgggtttg cgatgatttt gctttcagtt ccttcaagaa ctgcaactgc tgagtttgtt
      781 gtacctaagt caataccgat aattttagac atgtgttttt ctccttgtta gttaattttc
      841 tatttttctt tttgatactc ttcttaagtg gcgacgccgt cagagcttga ctttgtcaat
      901 atctttgact aaactttgag cctaaggtct caaaatttgc gcaaatagcg ccacagcgaa
      961 gagtatcgtc tttggttcgc ttcgctcact attacaaagc caaacatatt ttatctttct
     1021 tcatagttta ttgtcgtttc ggacaagttt tctattctgt aaattgcgac acgaggagtc
     1081 gaaatcgatt ttatttcgac gacgagttag taaggaagct agacaaacgc catagcgatt
     1141 gccgttttct gacgagttgc tttagctacc gtcagaattg ccgaatcgaa cacgccctaa
     1201 gctctgagta aaaaagatag attttcttgt gttcatcgaa cacttcgtca atctcctatt
     1261 tttactgcga gctttttacg ggctttgtat cttagttata caccactacc attgctgggc
     1321 gtaggatgcg gtcatggagt ttgtagcctt tttgaaagac ttgggcgatg gtatctactg
     1381 ggtgttcatc gtctgctggg agagtttgga tggccatatg gtagttatgg tcaaattcgc
     1441 catctgctgc gatttcttca attccttctt ctttcaaagc gtgaatcaag ctttcttgca
     1501 ccatccccaa gcccttcttc acatcatctg tcaaaccttc aactgcaagt gcacgctcaa
     1561 ggttgtcaag agatggtaag attgcttttg ccaagtcctg gctacgataa cgttgcaagt
     1621 tttgacgttc ttcattggca cggcgttgga tattttgcat ttctgcatga gcgcgaagat
     1681 atttgttttc gaactcatct gcacgttcat ttgccaagtc caactcagac ttttcaggag
     1741 ttgtttcttc agctgtttcc acaacttcct cttcttgaac ttcttctact tcttcatttt
     1801 ttatatcttg ggccatgttc gcctccttta atgatttcaa tcttaatgta cttcataatg
     1861 attactgctg aggtagcggt aaaaatctgt caacttcata gtcaaaacac ggttgaccac
     1921 attgacttga ttgattagct gttggtaatc cagattaact ggaccgataa tggctagaat
     1981 tccaactccc cgataaggaa tgaggaactt actactaatc accgctaggt cagctaaaca
     2041 ggactcttga ccgtctgcaa cacgaacatt ttgcatctgt tcctcacgca acccctcacg
     2101 aatctccaag gccacctttt gcggttggtc aaagaactga taggctgcta gattggcaaa
     2161 attcaagaga tggaccttgc ccgccatcac aatgttttcg ttgaacattt ccttaaagat
     2221 gtgttcaaag agatcgatga cattatccgt tgttgtaaag taacgctgga taatctgcgg
     2281 aatctccgtc cgaatcttgt agtgaatatc taaaacggtg tgaccgagga aacgttcctg
     2341 aatgatgctc ttcagtttca gcaaatcctc ctgcaagaag ttccttggaa tcagaaactg
     2401 actagtaacc gttcgcgact cgtctagggt aaataccgcc aaggctgtat gttgccccaa
     2461 aacaacgata tcaaaggctg tcaaacgttg cctgctcggc tcaacatcca gtgccactac
     2521 cgtacagcca ctcaggtctg ttagtaagtt agcagcctct tgcagaatat cctccaattt
     2581 gaagaattcc tgatcaaagg ctttgacaat ctcatatacc tcattttcag ccagccggtc
     2641 aaaatccagt gagtgtttca catagtactg aaaaccagca acacttggca tccgaccgct
     2701 tgaagtatga gccttctcaa gcaacccttg tttttctagt tccgccatgt cattacgaat
     2761 ggttgcactg ctagagttaa tagactcttg caaggctttt gatccgacag gttcgtgcgt
     2821 tttggtaaag atgtcaataa tcagatttaa aatatcctgc tgacgctctg taaccatctc
     2881 atcactcctc tctgtctgat caattagcac tcaatgcatc caagtgctaa cttatgattc
     2941 tattatacac ccttagaaga gaatgtcaag acaaaaactc aaaaaattag cactctttta
     3001 tcaagagtgc taaaaatcaa tctgaagacc tagaaccttg ctattcatct actcggtatt
     3061 tcctttttag tctgaaataa ccatgaatta gataagaaat cagaaaagca tataaaacaa
     3121 aaataagaaa gaaagacata gaaagatcct ctctaaacaa actcgtacaa acaacaagac
     3181 cacctaagaa agtagctgta caggaacgaa gtctagatcg cataacttcc catttgagcc
     3241 ccttactcat atagacttga tcctctggaa gtaaatcaga agacgattta cgaagaatcg
     3301 aacaagaacc tgctcctatc aattcccaac ctctatctct aaaatcttgc agttcatgct
     3361 tatatttttt aagaaatcta gaatcataga tacggtagat gacatcgtct ggttgacatt
     3421 ggtcaaaata gaacaaacca aaacgactcg ttctatacct ccaacctttc aaatgcatct
     3481 catgtaaata ttcttcttcc ttgtccaaat caacaatggt gaaaatccga aattctactc
     3541 tgctattcat tgtcttaccc caaaattaga aaacatgcct ggcgttattt attagataat
     3601 tctttccact tttgactcaa tctccaaaaa atataagaaa tctgaatcgc aaaaactatc
     3661 aataaaaccc aatctattat gaaaatcaaa aacactttcc aactgaaaga actacctcca
     3721 gtgacaaact ttgagaaaaa cggcagtaga gctaaaaaga gaagtaaaat aggaagcatc
     3781 cgcattgtta aaatccgttt ggcataaaaa aatctttatt taaacgaaaa tattatagca
     3841 aaatttacgc cagtttttga acggctgatg tagatatttt atactttcaa aatgtttaaa
     3901 tgtgattatt tatttttgaa aaatagctca ccagcccgac tgaaagtgct tatagaatga
     3961 taataagtcg cctgccgaaa acagcggaaa atagcggtgt tatgcggaga taatctgacg
     4021 cgatgcgaaa gtatattgca tacttatttt caacaattta gcagagtatt tttataagtg
     4081 tgatataata gaagtataat ttgttctgat agtttatttt atggagaagt agatttttag
     4141 aatgcggagg gttcaatatg gttgagttta taaagtctaa gaaagaaatg agtgaggagg
     4201 atattaaagc aaatttcatc actcctgcta ttgtatccaa aggatggaaa aatggtgagc
     4261 atatcgctta cgaagaatac ttcactgatg gtcgaattga ggttagagga gataaggctc
     4321 gtcgtaaaga aggaaaaaaa tcagactatt cactgtatta ccaatttgga actcgaattg
     4381 caattgttga ggcaaaggat aataaacaca gcgttcgagc aggattacaa caagctattg
     4441 aatatggaga gattttagat gttccatttg tttattcttc aaatggtgat ggctttattg
     4501 aacacgaccg tatcacgaga gaagaacgtg agctggagtt agacgaattc cctactcgtg
     4561 aagaattatt ttctcgtatg acgaaggaaa aaggattgac gtacgaaatt acagaagcta
     4621 tctcaactcc atactataca gacgccttct caatgaaaac gccacgctat tatcagcaaa
     4681 tagctatcaa ccgtactatt gaaacagttg ccagaggaca aaaacgagta atgtttgtga
     4741 tggcaacagg aacggggaaa acgttcatgg cttttcaaat tattcatcgc cttcgaaaag
     4801 ctggtttggc taaacgagtt ttattcttag cggatagaaa catcttagta gaccaaacga
     4861 tggctgaaga ctttaagcca ttcgaaaagg taatgacgaa aattacacca aaacttttga
     4921 ctgctcctga aaaattaaat tcttttgaaa tttatctagg gctttatcag caactaactg
     4981 gtgaagatgg aactgaaaca cattatcaaa aatttgacaa agacttcttt gatttaatcg
     5041 taattgatga agcgcaccgt ggttcagcta aggaaaacag taactggcgt aagataattg
     5101 attatttcag ttctgcgaca cagattggga tgaccgctac tcctaaagaa accaagaatg
     5161 cttccaatac ggaatacttt ggtgagccaa tctatactta tagtttaaaa cagggaatcg
     5221 aggatggttt tttggctcca tatcgtgtta tgagggttaa tttagatgtg gatgtggatg
     5281 gttatcgtcc agaaactgga aaagttgata ctaacggaca attaatagaa gataggtact
     5341 acggcaggaa agattttgat aaaaccattg tcattgatga tagaacgcaa agagttgcca
     5401 agtttgtttc tgattatatg aagcaaagca atgcacgatt tgataaaaca attgtttttt
     5461 gtgttgatat tgaccatgcc gagcgaatgc gtgctgcact tgtaaaagag aatctagact
     5521 tagtccaaga agactatcgt tatgtcatgc aagtaactgg tgacaacgct gaaggaaaag
     5581 ctcaactgga taactttatg gatgtcaatt ctaattttcc cgctattgta acaacgtcta
     5641 aattattaac gacaggagtt aatgctaaaa catgtcgttt gattgtttta gactctaata
     5701 tccaatccat gactgaattt aaacaaatta ttggtcgtgg cacacgtctt tatcctcaaa
     5761 aggggaaaga attttttacg attattgatt ttcgaaatgt taccaatttg tttgctgacc
     5821 ctgattttga tggtgatcct gtgaaggtgc tggaaacagg tgcgaaaaca gtcagtggtt
     5881 ctacgcccgg tttcgtagat gaggaaggtg acccagtaga aaaatatatc gttacagaca
     5941 agcaggttac cattcttaat tctactgttc aagtattaga tgaaaacggg aaactgatta
     6001 ccgaaagcct gaccgactac actcggaaga atatcttagg tagctacgcc actttgaacg
     6061 attttatcac agtttggcat acggcggata aaaagaagct tatcttagac gaactttata
     6121 aaaaaggagt ttatctagat gctattcgag agtcggaggg aatatcagaa caagaaatcg
     6181 atgattttga tttactccta aaacttgcct atggtcaaaa agaattaacc aaaacggaac
     6241 gtatcaataa actcaaacaa agcggatatt tatataaata tagtgaggaa gcgcgtgctg
     6301 ttttggaaat tttactgaac aaatacatgg ataaaggtat tggagaactc gaaagcattg
     6361 aaacattaaa acttccagaa tttcagatat atggtggaac cttcaaaatc atcaatactt
     6421 attttggaga taaaaaacga tatttacaag caattaaaga attggagcaa gagctattta
     6481 cagtagctta atgaaaggaa agtatgtcaa ttacatcatt tgtaaaaaga attcaagata
     6541 tcactcgaaa cgatgctggt gttaatggtg atgctcaacg tattgagcaa atgtcttggt
     6601 tattattctt aaaaatttat gatagccgtg aaatggtttg ggaattagaa gaagacgagt
     6661 atgagtcaat tatcccagag gaattaaaat ggcgaaattg ggctcatgct caaaatgggg
     6721 aacgggtatt gacaggcgat gaattacttg attttgtcaa taacaagtta ttcaaagagt
     6781 tgaaagagct tgaaataact tcaaatatgc ctattcgaaa aacgattgtt aaatcagctt
     6841 ttgaagatgc gaacaactat atgaaaaatg gcgtcttgtt acgccaagtc atcaatgtta
     6901 ttgatgaagt tgatttcaat agccctgaag atcgtcattc gtttaatgat atttacgaaa
     6961 aaattcttaa agatattcaa aatgctggga actcaggaga attttatacg ccacgtgcag
     7021 cgactgattt tattgccgaa gttcttaacc caaaacttgg agaatcaatg gcagaccttg
     7081 cttgcggaac aggaggcttc ttgacttcga ctctgaaccg tttaagtagt caacgtaaaa
     7141 ctagtgaaga taccaaaaaa tataatacag ctgtttttgg tattgaaaag aaagcatttc
     7201 ctcatctttt agcagttaca aatctgtttc ttcacgaaat tgatgaccct aaaattgttc
     7261 atggaaatac tttggagaaa aatgttcgtg aatatacgga tgatgaaaaa tttgacatta
     7321 ttatgatgaa tccacctttt ggagggtcag aattagaaac aataaaaaat aactttccag
     7381 cagaattacg gagttctgaa acagctgatt tatttatggc tgtcattatg tatcgtttga
     7441 aagaaaatgg tcgtgttgga gttattttac ctgatggttt tctatttggt gaaggtgtaa
     7501 aaactcgctt gaaacaaaaa ctggtagatg agttcaactt gcatacgatt attaggttgc
     7561 ctcatagtgt ctttgcaccg tatacaggaa tccatacgaa cattcttttc tttgataaaa
     7621 caaagaaaac agaagaaact tggttttatc gtttagatat gccagatggt tataaaaatt
     7681 tctcgaaaac taagccgatg aagtcagaac acttcaatcc tgttcgtgac tggtgggaaa
     7741 atcgtgaaga gattctggaa ggtaagttct acaaatctaa atcatttaca cctagtgaat
     7801 tggctgagtt gaattataat ttagaccagt gtggctttcc aaaagaggaa gaggaaatct
     7861 tagatccctt tgagttgatt cagaattatc aagcggaaag agcaacttta aatcataaga
     7921 ttgataatgt attagctgat attttgcagt tgttggagga caaataatga caccagaaca
     7981 acttaaagca agtattctcc aaagagcgat ggaagggaaa ttagtgccgc aaaatcccaa
     8041 tgacgaacct gcaagtgaat tattaaagag aattaaagct gaaaaagaaa aacttatcag
     8101 tgaaggaaaa atcaaacgag ataaaaagga aactgagata tttcgtggtg atgatg
//
LOCUS       DAVAAL010000082         8140 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVAAL010000082 DAVAAL010000000
VERSION     DAVAAL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.083"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(69..419)
                     /gene="rbfA"
                     /locus_tag="VQM60_001467"
     CDS             complement(69..419)
                     /gene="rbfA"
                     /locus_tag="VQM60_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET1081330.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(670..3462)
                     /gene="infB"
                     /locus_tag="VQM60_001468"
     CDS             complement(670..3462)
                     /gene="infB"
                     /locus_tag="VQM60_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET1081331.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(3479..3778)
                     /locus_tag="VQM60_001469"
     CDS             complement(3479..3778)
                     /locus_tag="VQM60_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET1081332.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(3771..4064)
                     /locus_tag="VQM60_001470"
     CDS             complement(3771..4064)
                     /locus_tag="VQM60_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET1081333.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(4086..5222)
                     /gene="nusA"
                     /locus_tag="VQM60_001471"
     CDS             complement(4086..5222)
                     /gene="nusA"
                     /locus_tag="VQM60_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET1081334.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(5266..5745)
                     /gene="rimP"
                     /locus_tag="VQM60_001472"
     CDS             complement(5266..5745)
                     /gene="rimP"
                     /locus_tag="VQM60_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET1081335.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(5871..6506)
                     /gene="trmB"
                     /locus_tag="VQM60_001473"
     CDS             complement(5871..6506)
                     /gene="trmB"
                     /locus_tag="VQM60_001473"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET1081336.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(6503..7297)
                     /locus_tag="VQM60_001474"
     CDS             complement(6503..7297)
                     /locus_tag="VQM60_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET1081337.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(7458..8069)
                     /locus_tag="VQM60_001475"
     CDS             complement(7458..8069)
                     /locus_tag="VQM60_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET1081338.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
BASE COUNT         2119 a         1927 c         1544 g         2550 t
ORIGIN      
        1 aagaatattt ttccatttca aattcaacct attttcctcc accaaaaaag aggggcaacc
       61 cctcttcttt agttcttatc cagattgcgt agcatctcgt caatcttgtt tccatactcg
      121 atggactcgt ctttgacgaa ggtcaaatct gggattttgt acaatttcaa attgcgacca
      181 agttcacgtt tgatggtacc agttgctttt tcaagcccga tttgggcttt ttggttatcc
      241 gaagcaaggt tactcaaaat ggtgtaataa accttggcaa cagacaagtc acccagcatc
      301 tgaacatctg tgatggtcac accttggaca cgtggatcac ggactttctt ttgcaaaatc
      361 tcattgactt cacgcttgat ttccatgccc acacgatctg tacggaaatg atttgccatg
      421 atattccttt ctctactttg aaccaaaagc taggccagca gacctagcta ttttaaactt
      481 cttgattttc agtttaaatt gaaacgaagt tcaatttgaa gtcatctgct tctttcgccg
      541 tggtgaaagt gatggaactg atttatcagt cccatcacag gggatttttg agaccctagg
      601 ctcaaaaata agcaatgaaa ccatcggttt gcttgcgtcc ctcaccacct aagaaaggag
      661 caaaaaatct tatctcttga tttcttccat gacatacgcc tcaatcacat catccatctt
      721 aatatcattg tagccgtcga tcatcaatcc accttcacga ccgtttgtca cttctttcac
      781 gtcgtcttta tagtgtttca agcttgcgag ttcaccatca tagataacga caccatcacg
      841 gataacacgg actttagagt cacgggcaac cttaccgttg ataaccataa atccaccgat
      901 agttcccact ttagacacct tgaaggtttc acggataacc gcttcaccaa taactttttc
      961 ttcaaattct ggatcaagca tccctttcat agcttcttcc atctcttcga taaccttgta
     1021 gataatgctg tgaagacgga tttccacatc gtcagcttct gcttgttgac gagcttgtgg
     1081 tgtagggcgt acgttgaaac caacgataaa ggcatttgaa gcttcggcaa gggtcacgtc
     1141 tgattcgttg atagcaccga ccgctgagtg gacgatagtc actttgacac cttccacgtc
     1201 aatcttttga agtgaggcag aaagggcttc aacagaacct tgtacatcag ccttgatgat
     1261 aacattaaca gatttgagtt ccccagcttt aagggtatca aagaggtttt caaggctaac
     1321 acgttgggta gcttgacgtt gtttcatgag ggcacgtttg gcacgctctt cacctgctgc
     1381 acgcgcagat ttttcatcct cgtaaacggc aaagtggtca cccgccatcg gtgcttcgtt
     1441 caaacctgtg atagagactg gtgttgatgg tccagcaact ttaacacgac gaccaaggtc
     1501 gttggtcata gcacggacac gaccgaaggt atttccgaca acgattgggt cttgaacatt
     1561 caaggtacct tgttgtacaa gaagggttgc gaccgcacct tttcctttat ccaagcgcgc
     1621 ttcgataacc gtaccgattg cacgcactgt tgggtctgcc ttgagttctt ggatttcagc
     1681 tacaagaagg actgtttcca acaattcttc gatattttgg ttgaatttag ctgagatttc
     1741 aacaaattca gaatctccac cccaagcggt tgacataact ccatgctctg ccaattcacc
     1801 gataacgcgt tctgggttag cacctggttt atcaatcttg ttaatggcta cgatgattgg
     1861 aacgttggcc gcttttgagt ggttaatggc ttcgatagtc tgaggcataa ccccgtcatc
     1921 tgccgctacg accaagatcg taatatcggt aacagaagca ccacgcgcac gcattgatgt
     1981 aaaggccgcg tgtcctggtg tatcaaggaa ggtaatcttc ttaccatttt ccacgatttg
     2041 gtaggcaccg atatgctgag taataccacc tgcttcacct gtcgcaacac gtgagttacg
     2101 aagagtatcc aaaagggttg ttttaccgtg gtcaacgtgt cccatgatag taacaactgg
     2161 tggacgctca accaattcat cttcattgag ataaccatct tcgacaaaga aacgttcgat
     2221 gtcagcatta tccacttcaa ccttttgttt ggcttcgata ccgtaatcca ccatgaggag
     2281 ttcaattgtt tccccatcta aggattggtt ttgtgtggcc atgacaccca tcatgaaaag
     2341 tttcttaacg atttcagctg gttcacgttt gatacgtttt gcgatttccg caacggtcat
     2401 accatctgta tattcaaatt ctgttggcaa ttcatggaat ttacgctccg taacaggttt
     2461 tggagtctga ttacggttgt tcttgttatt gccttttttg ttctttttgt tgttattcca
     2521 gttactattc ttttgatttc tcacttgatt ttgactactt cgattctttt gttgttttct
     2581 aggaccatct tcttcatgat cataatcgtc acgatttttg tctggtcgag cttgtttttt
     2641 acgacgtgta tccactgcag gttctttttt ctcagggacg acttcaacca ctgcttgaac
     2701 ttgctgtgct tgttcagcta acttagccgc ttcttcaaag atttcctctg gttccttgcg
     2761 tttgttagct tgagccaagg cttctttagc agcctgatac tgcttgaagc gttcctcact
     2821 tgaacgagcg tactctgcat tttgctctgc ttttagggct gctgcacggg ctttaaagtc
     2881 aatacgtgga gccgcttgat ttgaacgttt atcctcttgc tgacggcgct catttctacg
     2941 tttttgctga ccttgctgct tcttagcttg gtcattaaag cgacgattgt cgcggttgct
     3001 ttgaccttgt tttccaccat tacggccgtc gtttttctga cggtttccgt tttgttgttg
     3061 gtcacggtta ttgcccttat tttgcttgcg tcgttctgcc tgctctttag cacgggcttc
     3121 acgctcagcc ttgaaattac ggctttgcgg tttcgctgct actttttctg ttttcggagc
     3181 aactggctca gccggttttg cctcttcctt agctacagct ggtttagctg gctcagattt
     3241 ttcggctttc ttttctgcac ttacttttgg tgctgcaggt tttgcttcta ctttcggagc
     3301 agctgcaggc ttaaagctgg cagcaatttt tgcagcgaca gcttcttcca cacttgatga
     3361 gtggcttttc acatccaagc ccaactcttt tgcacgcgct acaacttctt tactttcttt
     3421 tccaagttct tttgcgattt cgtacaatct tttcttagac aaatcatgtc ctcctcttct
     3481 attccataag agacctcatt ttctttgtaa atccagcatc tgttacagcc aaaacctttc
     3541 tcgatttccc gactgctatg cttaattcca gtgttgaaaa cacggttaca atttctactt
     3601 gataataatg acttttatct tgaatcttct tggtcagatt gggtccagca tcatgagcta
     3661 gaaagaccaa cttggccttg ccgtcttgaa tggccttgac caccaattct tcacccgata
     3721 tgatgcgccc tgctcgctga gcaagcccca agagattact tatcttttgc ttattcaagt
     3781 cccaactctc ttcttttcac tttgtgatcc acataagcga tcaactcgtc ataaaagctt
     3841 tcttccactt ccatgctaaa gctgcggtta aagaccttct tctttttcgc ctctagggct
     3901 tctgcattgt ctagtttgat ataagcgccg cggccattgg ccttgcccgt aggatcaata
     3961 aagacttgtc cttccttgtt cttgacaatg cggagcaaat cacgcttatc aatcacttcg
     4021 ttagacacaa cagacttgcg caaagggatt tttcttgttt tcatctttcc ctcctctagc
     4081 agcttttatt cttctacagt atcgttttct acttccaact ctactgaagc agcgtcttcc
     4141 atggcttcaa attcgctagc agacttgata tcgatacggt aaccagtcaa gtgagccgcc
     4201 aagcgcacgt tttgtccacg acgaccaatg gcaagagaaa gcttgttatc tggaacaacc
     4261 accaaggcac gtttgctgtc gttttcatca aagataactt ggtcaacctc agcaggagcg
     4321 atggcattgt agataaattc agctggatct gctacccact cgataacatc gatattttct
     4381 tcgattggta ccatgcggtc atttttagca tcgtaacgag ctgggtggaa tttgctagta
     4441 atcttcttga tattagcacc accacgtcca acgattgtac cgatagcatc cacgtttgga
     4501 ttgtggctac gaacagcaac cttcgtacgg tcacctgctt cacgagccac gctcatgatt
     4561 tcaacagttc catcataaac ttctggaatt tcttgctcca tcaaacgttt gatcatttct
     4621 ggatggctac ggctaacaaa gacgttcaca ccacgagggt tgtcttcaac cttgtaaaca
     4681 taaacttcga tacgatcatg agaagcaaaa acttctccag gaatttggtc ttgttttgac
     4741 aattgggctt cgatgctacc aaggttgaca tagataaagc gattgtcaaa gcgttctact
     4801 gtaccagaca tgatttcttg ctcatgttct ttgtaagtat tgtaagtgat ggcacgtgtt
     4861 tgcttgcgca ttttttccat gatggtttgt ttggcagatt gggctgctac acgaccaaac
     4921 tcagctggtg cttcttcaaa cttgattttg tctccaagtt cataagctga attaatggca
     4981 agagcatctt tcaagctgat ttccaaacgg ctatcaaata cttcatcaac aacttcacgg
     5041 acagtataaa ctgtaaagtc acctgttttt tcgttgaagt caatagctac gctgtctgac
     5101 tgaccatagc gtctgcgata agcggaacga agcgactcta ctactgcgtc gatgatatct
     5161 tcttttttga ttcccttgtc ttcttccaaa atgcggaagg cctctagcat ttctttactc
     5221 atgttttctt cacttttgaa tcctcaaaag ctatcctttc tttttctata atttaactgc
     5281 taaacgtgct tttgatacta aactgtatgg aatttggacg gttttcttac gcgtcttgtc
     5341 catatattcc atagtcaact cgtcctcttc gaaggccaac aaggttcctt caaagacctt
     5401 ttgcttatcg atggcttggt agagcccgac atggatgtat tttccaaccg ctccagcgac
     5461 ggcatccttg gttttcaaag gacgttccaa acctggactg gtaatttcta ggaaatattg
     5521 ttctgggaag ggatctggct tgatggtgtc taggacagga ctgatcattt ctgtcaagtc
     5581 tgccgtgtcg ttcaaggtaa ttccttcggg tttatctaca aaaatactga gaatcatgtc
     5641 actgccaatc tttccatact cgatatccac gagttcaaaa ggagcttcta tgacaggttc
     5701 tacaacttct ctgactaatt ctacgattgt tgcgattgcg tccacctcct cataagcaag
     5761 aggcgaagat atttccccgc ctcacttttc atattcttac tatcagtata gcacgtttgc
     5821 caacttatgt aaagcaaaag cacttgtaca gcctaatttt aggttatctc ttaaaattct
     5881 gcctcaactc ggtagataac ttgccccttg tttgagaatt tttgctcgta ttctgtcatg
     5941 acattgcctt caaaatcact ggcatgcaaa tctaaccaga caccattgag tttcatgcca
     6001 tattgagaaa agctcactaa actgtactca aacaagccac ggttatccgt cttgaaatga
     6061 atttctccat tttcaggcaa gatacgtttg aaggtatcca agaaggtctt gtaggtcaaa
     6121 cgacgctttt catggcgttt tttcggccat ggatctgaaa agttcagata caagcgatca
     6181 atctcaccgt cttcaaagta gtcagtcaag tcagaaccat ctacccacaa gagcttgatg
     6241 ttagacactc cagcttcaag caccttgtcc aaagcgtagc tcaaaacaga cttttgaata
     6301 tcaatcccga tatagttgat gtcagggttt tgcttggcca tacctgaaac aaaggcaccc
     6361 tttccacttc caacttccac atgaatggga ttatcattgc caaacaagtc ccgccatttt
     6421 gccttggctt ccaagggatt gaggaccaca tactggggat ttgcctctag taattctgtt
     6481 gcccctttac gatttctaac tctcatcttc tctttccata cttgtctcgg aaatgacgca
     6541 aaccatgaat ctcccgattg acattttcta aatcttggtt catataatac ttggaaatct
     6601 gactcaaata agacaattga ccataccaat acaatttact taataccgtt tgattgtact
     6661 tgtaaccgta gtaggtcaac cattccttcc actgatgttc tgaaatataa tggcagagca
     6721 tatgggccac atcaaacatg cgatcggtca agcgaaccga atcccaatct actaaataaa
     6781 tcaagccact atctgtctca atccaattac tatgtcgtac atctccatgg acaatggtcg
     6841 catggtcctc tctaaatcct ggaatagtct gacgtaaatc agccatcact tcactgataa
     6901 aatgattttt acgcaaagca tctggagccg tttcttgcca agactgtagt aaatctacag
     6961 gtgtttccat ggcatagccc aaacgactca attgtgtcat caacggacgt gagcgatgaa
     7021 gacgggttaa aatattgacg atttgcttac gattcatatc atagggggtc aatatcttgc
     7081 ctgtcaacca ttcttgagca cacatatcac gcccatctgc caaacgacga ctccataata
     7141 attgtggagc aatttgttct ctagctagac caggtaggat tggagaggtg ttcattttta
     7201 caaagatgcg cttcccatca ggatagctac ccatataagc cttgccactt ttcccaggta
     7261 tgggagtcag tgttagctca ttatcaccca aatccatttc tttcctccgt ataaagtttc
     7321 cttactattc tactagtttt tggcctattc gtcaaccact ctaacccgat tctcaaagaa
     7381 aagcctctgg attggcttgg gtatcattat agcttgaaaa aggtaagcac cgtcttctgc
     7441 ttaccttttc ataattttta taaataatat aggagtggcc aactgttgta aaacatgtgg
     7501 actagagtag aaacccataa gttttggctc tttttataag caaggcccag caacaagccc
     7561 ccaagtagat aaaaaataaa cgaagggaca ttagaaggtc catgcatgaa agaaaagaga
     7621 gaggaagtta gcacaagtcc taaccaagaa ttgtcaaaaa ccgcaccttg aagaagtcct
     7681 ctgaaaatga gttcctcctg gatggggcca aaaactgagg tagtcaaaat caaagacagg
     7741 gaaattcctc tgctgacttc cgccaagtgt tggacccctg agccagaaga aacagcgaag
     7801 aaatgaatat aaaccagcgt ctcaagtaca cttagaagaa agattgcaat gaaaagcaaa
     7861 agttgtttct tgcttaacat cccaaaagaa atcatttcaa actttctagc ataagcaaca
     7921 ctcagcgagg ttacaagaag acttacaata atcaatgcag attcattttg aaaaaaatcc
     7981 ccatggttaa tcgtataagg aactgtaatg acaattatta gtactaaaaa tccaaaacac
     8041 aaggcatgaa atttatcaaa aaactccata aaattatcct cctcaacaaa cagacttcct
     8101 acacgtcatt ctttagttct agcctttcca aaacaaacca
//
LOCUS       DAVAAL010000083         8037 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVAAL010000083 DAVAAL010000000
VERSION     DAVAAL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.084"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            248..6679
                     /locus_tag="VQM60_001476"
     CDS             248..6679
                     /locus_tag="VQM60_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET1081339.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSNSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKAVVSKNPVTDNNTSNEEARIKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAVTTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALSSNGKKTDFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSKSKTMEGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNRTSLDQDPDLFAFNNQGVHAESTSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQKDLKVVS
                     REHFIRGILNSKGNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIPESKFKNLKNVKDDSLNKETAEVENNLLVDNQSIEG
                     KSLFNIHKTISTIRDFENKDLKKLIKKKYKQEDDFVNGGTRTVERDYKYDDKGNIIAY
                     DDGTDLEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKSIEI
                     KATKYDFHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDNDQKKAEIKIRMPEKIKET
                     KSEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQ
                     REDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVG
                     IFLGLKKKNQD"
     gene            7311..7787
                     /locus_tag="VQM60_001477"
     CDS             7311..7787
                     /locus_tag="VQM60_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET1081340.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            7825..>8037
                     /locus_tag="VQM60_001478"
     CDS             7825..>8037
                     /locus_tag="VQM60_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HET1081341.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVI"
BASE COUNT         3113 a         1141 c         1532 g         2251 t
ORIGIN      
        1 ctctttgatt ttcattgagt actaatctta ttttttaacg tgtgctaaaa aataaattaa
       61 tgcatgttaa tttttcttga attaaatttt tttaaatgat atactgtttt tatggagaga
      121 taacaattat atataaatgt actactctat ttcctagata atacttagta aaacttttta
      181 taacaaaggc tagcaaagtt aaagtttatt atctattgga agttaaataa atatgtaagg
      241 aattaaaatg aaaaaaagta cagtattgtc attaaccaca gctgcagtta ttttagcagc
      301 ctatgcccct aatgaggtag tcttagcaga cacatctaac tctgaagatg ctttaaacat
      361 ctctgataaa gaaaaagtag tattagataa ggaaacagaa aataaagaga aacatgaaga
      421 tattcataat gctatagaaa cttcacagga ttttaaagag aagaaaacag cagtcattga
      481 gggaaaagca gttgttagta aaaatcctgt gacagacaat aacactagca atgaagaagc
      541 aagaatcaaa gaagattcca atcaatccca aggagatcat acggactcat ttgtgaataa
      601 aaacacagaa aatcccaaaa aagaagataa agttgtctat attgctgaat ttaaagataa
      661 agaatctgga gaaaaagcaa tcaagcaact atccaatctt aagaatacaa aagttttata
      721 tacttatgat agaattttta acggtagtgc catagaaaca actccagata acttggacaa
      781 aattaaacaa atagaaggta ttacatcggt tgaaagggca caaaaagtcc aacccatgat
      841 gaatcatgcc agaaaggaaa ttggagttga ggaagctatt gattacctaa agtctatcaa
      901 tgctccgttt gggaaaaatt ttgatggtag aggtatggtc atttcaaata tcgatactgg
      961 aacagattat agacataagg ctatgagaat cgatgatgat gccaaagcct caatgagatt
     1021 taaaaaagaa gacttaaaag gaactgataa aaattattgg ttgagtgata aaatccctca
     1081 tgcgttcaat tattataatg gtggcaaaat cactgtagaa aaatatgatg atggaaggga
     1141 ttattttgac ccacatggga tgcatattgc agggattctt gctggaaatg atactgaaca
     1201 agatatcaaa aactttaacg gcatagatgg aattgcacct aatgcacaaa ttttctctta
     1261 caaaatgtat tctgacgcag gatctgggtt tgcgggtgat gaaacaatgt ttcatgctat
     1321 tgaagattct atcaaacaca acgttgatgt tgtttcggta tcatctggtt ttacaggaac
     1381 aggtcttgta ggtgagaaat attggcaagc tattagggca ttaagaaaag caggcattcc
     1441 aatggttgtc gctacgggta actatgcgac ttctgcttca agttcttcat gggatttagt
     1501 agcaaataat catctgaaaa tgaccgacac tggaaatgta acacgaactg cagcacatga
     1561 ggatgcgata gcggtcgctt ctgctaaaaa tcaaacagtt gagtttgata aagttaacat
     1621 aggtggagaa agttttaaat acagaaatat aggggccttt ttcgataaga ataaaatcac
     1681 aacaaatgaa gatggaacaa aagctcctag taaattaaaa tttgtatata taggcaaggg
     1741 gcaagaccaa gatttgatag gtttggatct taggggcaaa attgcagtaa tggatagaat
     1801 ttatacaaag gatttaaaaa atgcttttaa aaaagctatg gataagggtg cacgcgccat
     1861 tatggttgta aatactgtaa attactacaa tagagataat tggacagagc ttccagctat
     1921 gggatatgaa gcggatgaag gtactaaaag tcaagtgttt tcaatttcag gagatgatgg
     1981 tgtaaagcta tggaacatga ttaatcctga taaaaaaact gaagtcaaaa gaaataataa
     2041 agaagatttt aaagataaat tggagcaata ctatccaatt gatatggaaa gttttaattc
     2101 caacaaaccg aatgtaggtg acgaaaaaga gattgacttt aagtttgcac ctgacacaga
     2161 caaagaactc tataaagaag atatcatcgt tccagcagga tccacatctt gggggccaag
     2221 aatagattta cttttaaaac ccgatgtttc agcacctggt aaaaatatta aatctacgct
     2281 taatgttatt aatggcaaat caacttatgg ctatatgtca ggaactagta tggcgactcc
     2341 aatcgtggca gcttctactg ttttgattag accgaaatta aaggaaatgc ttgaaagacc
     2401 tgtattgaaa aatcttaagg gagatgacaa aatagatctt acaagtctta caaaaattgc
     2461 cctacaaaat actgctcgac ctatgatgga tgcaacttct tggaaagaaa aaagtcaata
     2521 ctttgcatca cctagacaac agggagcagg cctaattaat gtggccaatg ctttgagaaa
     2581 tgaagttgta gcaactttca aaaacactga ttctaaaggt ttggtaaatt catatggttc
     2641 catttctctt aaagaaataa aaggtgataa aaaatacttt acaattaagc ttcacaatac
     2701 atccaacagg cctttaacct ttaaagtttc agcatcagcg gtaactacag attctctaac
     2761 tgacagacta aaactggatg aaacatataa agatgaaaaa tctccagatg gtaagcaaat
     2821 tgttccagaa attcatccag aaaaagtcaa aggggcaaat attacatttg agcatgacac
     2881 tttcactata ggggcaaatt ctagctttga tttaaatgcg gttataaatg ttggggaggc
     2941 taaaaataaa aataaatttg tagaatcatt tattcatttt gagtcagtag aagaaatgga
     3001 agcattaagc tccaatggca agaaaaccga cttccaacca tctttatcaa tgcccctaat
     3061 gggatttgct gggaattgga accacgaacc aatccttgat aaatgggctt gggaagaagg
     3121 atcaaaatca aaaacaatgg aaggttatga tgatgacggt aaaccaaaaa ttccaggaac
     3181 cttaaataag gggattggtg gagaacatgg tatagataaa tttaatccag caggagttat
     3241 ccaaaataga aaagataaaa atagaacatc tctagatcaa gatccagact tatttgcctt
     3301 taacaaccaa ggtgttcatg cagaatccac tagtggttct aagattgcta acatttatcc
     3361 tttagattca aatggaaatc ctcaagatgc tcaactagaa agaggactaa ctccatctcc
     3421 acttgttcta agaagtgcag aagaaggact aatttcaatt gtaaacacca acaaagaagg
     3481 agaaaaccaa aaagacctta aagttgtatc aagagagcac ttcataagag gaattctaaa
     3541 ttctaaggga aatgacgcta agggtatcaa atcatccaaa ctaaaagttt ggggtgactt
     3601 gaaatgggat ggactcatct ataacccaag aggtagagaa gaaaatgccc cagaaagtaa
     3661 ggataaccaa gatccagcta ctaagatacg aggacaattt gaaccgattg cggaaggtca
     3721 atatttctat aaatttaaat atagattaac taaagattac ccatggcagg tttcctatat
     3781 tcctgtaaaa attgataaca cagcccctaa gattgtttcg gttgattttt caaatcctga
     3841 aaaaattaag ctaatcacaa aggatactta tcacaaggta aaagatcaat acaagaatga
     3901 aactttattt gctagagatc aaaaagaaca tcctgaaaaa tttgatgaga ttgcaaatga
     3961 ggtgtggtat gcaggagctg ctcttgttaa tgaagatgga gaggttgaga aaaatcttga
     4021 agtaacttat gcaggtgagg gtcaaggaag aaataggaag cttgataaag atgggaatac
     4081 tatctatgaa attagcggag caggagatct aagaggaaaa atcatcgaag taatcgccct
     4141 agatggagca agcaacttta ccaaaatcca taggattaaa tttgctaatc aagctgatga
     4201 aaaagggatg atttcctatt atctagtaga tcctgatcag gattcatcta aataccaaaa
     4261 gcttggcgaa attcctgaat ctaaatttaa gaatttaaaa aatgttaaag atgatagcct
     4321 aaacaaagag acagctgagg tggaaaataa ccttttagta gataatcagt ctatcgaagg
     4381 aaaatctctc tttaatattc ataaaactat ttcaacaatt agagactttg aaaataaaga
     4441 cctaaagaaa ctcattaaaa agaaatataa gcaagaagac gactttgtaa atggtggaac
     4501 aagaacagta gagcgtgatt ataaatacga tgataaagga aatataatag cctacgatga
     4561 tgggactgat ctagaatatg aaactgagaa acttgacgaa atcaaatcaa aaatttatgg
     4621 tgttctaagt ccgtctaaag atggacactt tgaaattctt ggaaagataa gtaatgtttc
     4681 taaaaatgcc aaggtatatt atgggaataa ctataaatct atagaaatca aagcgaccaa
     4741 gtatgatttc cactcaaaaa cgatgacatt tgatctatac gctaatatta atgatattgt
     4801 ggatggatta gcttttgcag gagatatgag attatttgtt aaagataatg atcagaaaaa
     4861 agctgaaatt aaaattagaa tgcctgaaaa aattaaggaa actaaatcag aatatcccta
     4921 tgtatcaagt tatgggaatg tcatagaatt aggggaagga gatctttcaa aaaacaaacc
     4981 agacaattta actaaaatgg aatctggaaa aatctattct gattcagaaa aacaacaata
     5041 tctgttaaag gataatatca ttctaagaaa aggctatgca ctaaaagtga ctacctataa
     5101 tcctggaaaa acggatatgt tagaaggaaa tggagtctat agcaaggaag atatagcaaa
     5161 aatacaaaag gccaatccta atctaagagt cctttcagaa acaacaattt atgctgatag
     5221 tagaaatgtt gaagatggaa gaagtaccca agctgtatta atgtcggctt tggacggctt
     5281 taacattata aggtatcaag tgtttacatt taaaatgaac gataaagggg aagctatcga
     5341 taaagacgga aatcttgtga cagattcttc taaacttgta ttatttggta aggatgataa
     5401 agaatacact ggagaggata agtccaatgt agaagctata aaagaagatg gctccatgtt
     5461 atttattgat accaaaccag taaacctttc aatggataag aactacttta atccatctaa
     5521 atctaataaa atttatgtac gaaatccaga attttattta agaggtaaga tttctgataa
     5581 gggtggtttt aactgggaat tgagagttaa tgaatcggtt gtagataatt atttaatcta
     5641 cggagattta cacattgata acactagaga ttttaatatt aagctgaatg ttaaagacgg
     5701 tgacatcatg gactggggaa tgaaagacta taaagcaaac ggatttccag ataaggtaac
     5761 agatatggat ggaaatgttt atcttcaaac tggctatagc gatttgaatg ctaaagcagt
     5821 tggagtccac tatcagtttt tatatgataa tgttaaaccc gaagtaaaca ttgatcctaa
     5881 gggaaatact agtatcgaat atgctgatgg aaaatctgta gtctttaaca tcaatgataa
     5941 aagaaataat ggattcgatg gtgagattca agaacaacat atttatgtaa atggaaaaga
     6001 atatacatca tttgatgata ttaaacaaat aacagacaag acactaaaca ttaagattgt
     6061 tgtaaaagat tttgcaagaa atacaaccgt aaaagaattc attttaaata aagatacggg
     6121 agaggtaagt gaattaaaac ctcatagggt aactgtgacc attcaaaatg gaaaagaaat
     6181 gagttcaacg atagtgtcgg aagaagattt tattttacct gtttataagg gtgaattaga
     6241 aaaaggatac caatttgatg gttgggaaat ttctggtttc gaaggtaaaa aagacgctgg
     6301 ctatgttatt aatctatcaa aagatacctt tataaaacct gtattcaaga aaatagagga
     6361 gaaaaaggag gaagaaaata aacctacttt tgatgtatcg aaaaagaaag ataacccaca
     6421 agtaaaccat agtcaattaa atgaaagtca cagaaaagag gatttacaaa gagaagatca
     6481 ttcacaaaaa tctgattcaa ctaaggatgt tacagctaca gttcttgata aaaacaatat
     6541 cagtagtaaa tcaactacta acaatcctaa taagttgcca aaaactggaa cagcaagcgg
     6601 agcccagaca ctattagctg ccggaataat gtttatagta ggaatttttc ttggattgaa
     6661 gaaaaaaaat caagattaag acaaaagcta tagaaaaaaa tggtttatgt actgagatta
     6721 gatagtgagg tgatgacata gttttgtgaa aacggccatt tataactcaa ttatttagtt
     6781 tacttcactt cactattgat actatttgga gttattaatg gacttagttt agataactaa
     6841 tgaattgatt gaaagggtta gtattgacaa tattggtcat attgattaga aaatagagtc
     6901 tatcaaaatt taaaggctaa tagaggtgat gagacaattt caaggaatta agtagcgttt
     6961 ggttgcttaa ttccttttta tttggaagga agtttttcgt aaccgcccaa taacgaagtc
     7021 tattggacgg ttacgatatg atcactgact tcgtcagtct tatctacaac ctcaaaactg
     7081 tgttttgagc aacctgcggc tagcttccta gtttactctt tgattttcat tgaatattag
     7141 aacagcaaaa atgcttggag tatttgtttg tgtgtttatt tttatataac aaactataaa
     7201 caaaacaaaa atataaaaaa agagacaaaa aagaacaaaa agtaattgac aacggtttca
     7261 tatagtgtta tacttatggc ataaagttaa tggaaagaag ggaaaaactt atggtattag
     7321 attatttctt tgacaaaaac cttgtgtttt gcttagaagc ggataatcaa gaacatctct
     7381 ttgatcaggt agcaagcttg ttggaagaac gagaaattgt cactccaact tatcgtgaag
     7441 ccttgatcac gcgtgaaaag tcatttccaa ctggtttaga tatggaattt ctaggaaagg
     7501 acttgccaaa tgtagcgatt cctcacacag atattgttca taatctagct gagaaagtgg
     7561 tggttgttcg attagaaaaa ccagtaactt ttcacaatat gatagctcct gataaggaag
     7621 tagaagtatc tctactcttc tttatcatta acaattcaag ttcaagtcaa acgaacattc
     7681 tggctcagtt gatggacttt ttcacaggaa atggtcatct tgaagaccta tcaaaaattt
     7741 ctgaaccaga aaaactttat gcttacattg ctgaagcaac cgcttaatct tgtctattaa
     7801 aaaataaaat cggaggaaat ccaaatgatt aaaattcttg ctgcctgcgg tgcaggtgtt
     7861 aactcaagtc accaaattaa aagtgctcta gaagaagaac tttcaaaccg tggttatgat
     7921 gttcactgtg atgcagtcat ggtgaaagat gtaaacgaag accttatgaa aggttacgat
     7981 atctttacac caatcgctgc aacagacctt ggttttgaac caggtatccc agttatc
//
LOCUS       DAVAAL010000084         7838 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVAAL010000084 DAVAAL010000000
VERSION     DAVAAL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.085"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(185..1153)
                     /locus_tag="VQM60_001479"
     CDS             complement(185..1153)
                     /locus_tag="VQM60_001479"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET1081342.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1298..2113)
                     /locus_tag="VQM60_001480"
     CDS             complement(1298..2113)
                     /locus_tag="VQM60_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET1081343.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPD
                     STFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLI
                     FLGLALGTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2138..2635)
                     /locus_tag="VQM60_001481"
     CDS             complement(2138..2635)
                     /locus_tag="VQM60_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET1081344.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2708..4069)
                     /gene="radA"
                     /locus_tag="VQM60_001482"
     CDS             complement(2708..4069)
                     /gene="radA"
                     /locus_tag="VQM60_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET1081345.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINETAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4083..4598)
                     /locus_tag="VQM60_001483"
     CDS             complement(4083..4598)
                     /locus_tag="VQM60_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET1081346.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4600..5043)
                     /locus_tag="VQM60_001484"
     CDS             complement(4600..5043)
                     /locus_tag="VQM60_001484"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET1081347.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5100..5197)
                     /gene="ffs"
                     /locus_tag="VQM60_001485"
     ncRNA           complement(5100..5197)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VQM60_001485"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5230..5697)
                     /locus_tag="VQM60_001486"
     CDS             complement(5230..5697)
                     /locus_tag="VQM60_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET1081348.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSKESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5898..7184)
                     /locus_tag="VQM60_001487"
     CDS             complement(5898..7184)
                     /locus_tag="VQM60_001487"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET1081349.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7415..7651)
                     /locus_tag="VQM60_001488"
     CDS             complement(7415..7651)
                     /locus_tag="VQM60_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET1081350.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
BASE COUNT         2501 a         1915 c         1259 g         2163 t
ORIGIN      
        1 cgtcgattca agtgctatca ctgttcaaaa atgatggtcg ctgaaacttc tgatgacgta
       61 cagtcatatt tcttttcttt ttattatatc acagttttaa atctagcttt actagattca
      121 ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat ctcaggcttg
      181 gtcattaaat ttttttctca atatcgaaaa gtggagaaag tggtcgtttt tcatgaatac
      241 gtacgatagc atcccctagg agatgagcga ttgaaatctg ctcaatctta tcaatcaaac
      301 gctcttctgg cagatagatg gtatccaaaa caaccaattt cttaatagct gatttttgga
      361 tattgtccgt agcaggacca gaaagaactg ggtgcgtaca gcttgcatag acttcaacag
      421 caccagcttc cgcaagagca tctgccgcat gacaaatcgt tccagcggta tcaatcatat
      481 catcaatcaa gatacaagtc ttgccttcaa ccttaccgat gatgttcata acttcactag
      541 tattcatctt atcaacgctg cgacgtttat caataatagc gatagatgtt ttcaaaaatt
      601 ctgccaactt acgagcacga gtcacccctc catggtccgg gctgacaacc acatagtcag
      661 aaccaaccat accacgacgc tcaaaataat ctgcaatcag aggagcaccc atcaaatgat
      721 ccacaggaat atcaaagaat ccttgaattt gcgcagcatg caagtcgatg gtcaataaac
      781 gatccactcc agctacttca agcatatttg cgacaagttt tgaagtgatt ggctcacgcg
      841 ctctcgcctt tctatcctga cgtgcatacc catagtaagg catgacaaca ttgacagatt
      901 ctgcactcgc acgcttcaaa gcatctacca taatcaaaat ttcaagcaga ttgtcattta
      961 caggcgaact agttgattgt aggataaaga cgtgtttccc acggattgat tcttcaatgt
     1021 tgacctgaat ctctccatct gaaaattggc gaacacttga tttccccaac tctatcccaa
     1081 tctcctgcgc cacacgttct gccaattctt tattagaaga aagggcaaac agctttaaat
     1141 cagaaaaaga catgatttcc tccggtatat atgtataact tgtgcctttc acaagatttt
     1201 ccatctacca ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa gaagggcacc
     1261 atatttgtac ccttgcatca ttcttttgaa aaatattcta ggtcatcaac tcattgtgtt
     1321 tctcaacaaa gcaataagca tgataaaaac catagagagc aatagccgta accactggaa
     1381 tcgctaaagg caactctgtt tccaactcca caaaaggaga gttaaacaag aagtgagttc
     1441 ccaaggctaa acctagaaaa ataaggccct gtttcttgcc aaccttctgt cctttatagg
     1501 ctctgtaaag caagtaaaca cctactacag ctagacctga aaaagtccag tgagaggcaa
     1561 ttcctgagat gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc tctggcaaat
     1621 ccgtacgaat ataaccaata tcctcaatca tttggaatcc caaaccggaa gcaattccaa
     1681 gtaaaaacaa agattttaat tttcgcacag gaatcaaagc caaaacaaaa acaagtggca
     1741 ataatttcaa gggttcttct accaaaggag ccacaatagc actttcaaag gcatttaaaa
     1801 atgtactatc tgggaaaaga acccccagta aatcatggat ataagtatta gcaaaactag
     1861 acaaccagcc tgaaaggaac atccctccca ataaagacag aatcaaaacc ttctttggca
     1921 attcccattt tttcccaata cggaagagaa aataaagagc cggaatcatg taaaagagag
     1981 ctagaaagat agaaactccc attagtccat attccgcacc tgacctcgaa ccgtccgtat
     2041 agtagatggt ttcatactgt aaaccaatac atagcaataa aataaaaata aataaaatac
     2101 tgctcttctt catacacttt ctttctaaat gaagtattta taattctacg actgtcatac
     2161 ttcctgtatc aacattgtaa atagcaccag agataatgac atcgtctggt attaggggag
     2221 actcgataag cagttgcata tcctcgcgta cactctcttc tatatcttgg aagggcaaga
     2281 agtcctggtc tgacacatct acacctaatt cctcttttaa atactcctga aaaggttcat
     2341 tttcaaaggt ctgagcacca cagtctgtat ggtgcaatac cacaatctct cttgtcccca
     2401 tttgttgctg ggaaataact agcgaacgaa tcatgtcttc agtcactcga ccacctgcat
     2461 tccgcaaaat atgagcatcc ccaagtgcca agcccagagc ttgcgcaacg tgcaaacgtg
     2521 agtccataca ggtcacaatg gctactctgg ttttaggttt aagtggcaga tttaactgcc
     2581 catgtagggc aacataagcc tgattggctt gcataaactg ttcaaaatac gacacgattc
     2641 cctccttgaa aatttgatag tcaaatattt ctcctatctt atcattttta agagaatttg
     2701 tcacggatta tgcaaagacc tttttcaaaa cttcctgaat cgttgtcacg ccaatgacct
     2761 gaatttcctt aggcagagtg attcctgtca aggaattctg aggtacataa atcttagtaa
     2821 agcccagttt agcagtttcg ttgatgcgtt gctcaatacg attcacgcgc cgaatctctc
     2881 ctgtcaagcc cagttctccg acaaaacatt cctgaggatt agttggcttg tctttgtagc
     2941 tcgaagcaat agcaactgca acagccaagt caatggcagg ttcatccaat ttaacaccac
     3001 cagcagattt gagataggca tcctgatttt gcaagagaag ccctgcccgt ttttccaaaa
     3061 cagccataat caagctagca cggttaaaat caagtcctgt cgtagtacgc ttggcatttc
     3121 caaacatggt cggtgttacc aaagcctgaa cctccgccaa aatcggacgc gtcccttcca
     3181 tggttacaac gatggaggaa ccagtcgccc catccaaacg ctcttctagg aaaacttgac
     3241 tcggattgag tacctcaacc aagccgcccg actgcatctc aaaaatccca atctcattag
     3301 tggaaccaaa acgatttttg accgctctca aaatacgaaa ggtgtggtga cgctcccctt
     3361 caaagtaaag caccgtatcc accatatgct ccaacatacg aggcccagcc aaggttcctt
     3421 ctttggtcac atgacctacg ataaagatgg caatgttatt ggtcttggcc aactgcatga
     3481 gttcagcggt cacttcacgt acctgagaaa cagacccctg cacccctgaa atctcaggag
     3541 acatgatggt ctggatggaa tcaataatga gaaagtctgg ctggatacgc tccacttctg
     3601 cacgaacact ctgcatattg gtctctgcat agagataaaa ctcactatca atatcaccta
     3661 agcgctctgc acgtagttta atctgctggg cagactcctc cccactgaca tagagaactg
     3721 tccccacttg ggacaactgg gttgagactt gtaggagaag agttgatttc ccaatcccag
     3781 gatccccacc gatgaggacg agacttcctg gtaccactcc gcctccaagc acacggttga
     3841 attcctccat ctccgtcttg gttcgattga cattgatgga agtcacctca gctagtttca
     3901 tgggcttggt tttctcacct gtcaaggaca cacgcgcatt cttgacctcg gcaacctcaa
     3961 cctcttccac aaaagaagac caagacccac agttggggca acgtcccaga tatttagggg
     4021 aattataccc acaattttga catacaaatg tcgctttttt ctttgcgatg acaaacctct
     4081 ttctatatct ctaactcaca ctcaatcact tggcaaaaat caatcttctc atttggcaca
     4141 aactggcgca tgagcattcg atgagcaaca actaccacag tctgatgttc tcgatactta
     4201 gacatacatt ctagaaaccg agacttcatt tccgtagctg tctcatattg aataggacta
     4261 ttaggaagca actccccctt gttttctaaa aacagtcttc tagctgtttc aaagttttct
     4321 attcctgttt tatagacctg ccattcatgt aataaaggct ctactcttaa aggaagaccc
     4381 gtagcacaga ccacatacga agccgtttct aaagctcttg tgactgcaga agatacgatt
     4441 atttcagctg acgagagtaa aggatttttg ctcaatttct ggacttgctg ccgtcccatc
     4501 tcagacaagg gtgccaaatc tatcccaaat cctatataag aacgctcctc taactcacgg
     4561 taatctggct ccccatgacg tacaaagata atcttcattc tagtgccctg tcgatccaaa
     4621 tccaccagtt cgaacgccat cagctgcatc tccatctgca attaagaaag tagcaaaaac
     4681 agcctggaca atacgttccc caacttcaag aacaacctct tggtctgtga tattcttcat
     4741 ctgcgcaaaa atatgccctt catttccagg atttccataa taatccccat caatgacccc
     4801 aactgagtta attaaaacca agcccttctt acgaggattt gaagaacgat catagaggta
     4861 gagaacctca gtcggctgca tataagcctt aacccctgtc ggaaccaaga caatctctcc
     4921 tggcgcaaca actgtacgca cagcaacctt taagtcgtaa ccagccgcat gcgctgtctc
     4981 acgcttgggc aataaatttt catctgtaaa actcgaaacc aattcaaaac cacgaatttt
     5041 cataattttc tcttttctat tatcatttat tctagattat tctatcttat ttattcggaa
     5101 aaagcacgaa aaaaagagca cacaattcaa atcgcttagg gctgctggat tcctcccctg
     5161 acccgcttca cgcagaactg ttgctccact atttattata tcacattcct attcatttta
     5221 aaagcaaaat tattttttcc gtctatttct aaaaaaatcc tgcatgatag ctgcgcattc
     5281 atcttccaaa attcctattt caacctccac acgatgattg agacgctcat ctgtcaagat
     5341 atcgtacaaa ctcccagcag cgccaaattt ctggttttta gccccataga ccacatttgg
     5401 aatgcgggca agcccaatcg ccccactaca catgacacaa ggttcaatgg tcacaaaaag
     5461 tgtgcaatcc agcaagcgcc agctctcctt actcaagttc gcatcctcta tagccataat
     5521 ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca ttatgcccac gaccaatgat
     5581 ttccccatct ttgacaatca cacaaccaat tggaatttca tcgtgttcaa gagcaatctc
     5641 agcctctctc aaagcctccc tcataaagac ttctttttct tcaagcgtat aatacatctc
     5701 ttttctcttt tcctacttat cgattttatt attatatcat gaatcccaac acaaaagaag
     5761 ccaccgaatg cggtgactct atagggagat tattatgaaa aagaaaagtt taggatattt
     5821 gttacaacaa gttaggaggt cttcttgtaa ccgtctatag tatacccgac ctatcttaaa
     5881 caaatcttaa aaatctctta ggaccaaaca ctttctaaaa tatttgtttg ttcacgacca
     5941 ggacctactg agaaagtaga aatacgaacg ccaaccaatt cactcacacg acgaacatag
     6001 ttacgcgcat tctcaggaag atcttccaaa ttgcgaactc cggtaatatc ttctgaccaa
     6061 cctggcaact cttcatagat aggcttgcaa cgtttcaatt gctcaagact agctggatag
     6121 tagtcaatac gttgaccgtc aagatcatag gccacacaga ttttcacagt atccaaaccg
     6181 ctcaaaacat caatagagtt caaagaaagg ttagtaatac cagaaacacg acggctatga
     6241 cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac gaccagttgt tgtaccatac
     6301 tcatgaccca cttcacggat acgttctccc acttcatcaa acaactcagt tgggaaagga
     6361 ccatctccta cacgactcgt ataagcttta catacaccta caaccttgtc aatcttgctt
     6421 ggaccgacac cagaaccaat tgtcacacca ccagctacag ggtttgatga cgtaacaaat
     6481 ggataagtac cttggtcgat atctagcata acaccttgtg caccttcaaa aagcacacgt
     6541 ttgccattat caagcgcatc attcaagata acagatgtat ctatcacgta tttcttgatt
     6601 tgttgaccat attcgtaata ttcttcaaaa atatcatcga aaacaatcgc tttactgtca
     6661 tacaattttt caaaaagacg attcttttca gcaaggttac gttctaaacg ctcacggaaa
     6721 atatctttat ctaaaagatc tgcaatacga attccaacac gagcagcctt gtccatataa
     6781 gctggaccaa ttcccttaat tgtcgtacca atcttattgt cgcccttagc ttcttcttgc
     6841 aagcgatcca actcgatatg ataaggcaaa ataacatgcg cacgatcaga aatacgcaag
     6901 ttatcagttg ttacaccttc ctcatgaaga tagctcaact cttttacaag agatttagga
     6961 tttacaacca taccattccc aatgacagat attttttcag ggaagaaaat cccagatgga
     7021 atcaagtgca acttaaattt cttaccgtca atcacaatcg tgtgaccagc attatcacca
     7081 ccttggtaac gtgcaatcac ttctgcattc gctgaaagga agtctgtaat cttcccttta
     7141 ccttcatcac cccattgggt acctacaaca acaactgaag tcataatttt gtctgagccc
     7201 tcaggctctt cctttctcac atacatggca ggactctcac ctgcaattat atcttacaat
     7261 ttattataat aaaaaatcgc ctttttatca agaagaaaca atagaaagat ttgctatttc
     7321 caactattaa aaaatgattt agaaaaatta ctagctattt actattatct ttccataaaa
     7381 gagtaaatta gttcggaaat ttactaaaat tacctcaaca agaaataaaa ccccgattca
     7441 ttaccaattt ttcaagatac aaacgataag caacacgata atggtaaacg ataaaatccc
     7501 tatgacaacc aatgccatat ctcactaaat aataaattaa aaatttaaaa tgaacatgtt
     7561 cccagtcaaa attatcacca aatgtaggac catactcttc ttcaatacta tcatagaaat
     7621 tagccatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct tttttataaa
     7681 cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt aaaataccag
     7741 cctcaacaag ataaaaatag gaaaagttga caaaatagaa acaaatttgc tacctaaatt
     7801 tcaaacaatt aaagttctaa aaagaccgcc ttaaaact
//
LOCUS       DAVAAL010000085         7828 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVAAL010000085 DAVAAL010000000
VERSION     DAVAAL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7828
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.086"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..704)
                     /locus_tag="VQM60_001489"
     CDS             complement(<1..704)
                     /locus_tag="VQM60_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET1081351.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPR"
     gene            complement(704..2869)
                     /locus_tag="VQM60_001490"
     CDS             complement(704..2869)
                     /locus_tag="VQM60_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET1081352.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            3039..3992
                     /locus_tag="VQM60_001491"
     CDS             3039..3992
                     /locus_tag="VQM60_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET1081353.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVALGQYRLDSIHLDTYSLAF
                     TLFTIPFWILQGTAEELVTRAWLIPQLAKRTNLKVAIIISSSLFTLLHLGNPGITFLS
                     AIDLFLFGVAMSLYLLKTDTIWGIGGIHGAWNFAQGNLFGVLVSGQSSGTSIMKFTPQ
                     GNQDWLSGGSFGIEGSIVSSIILFLLILYLAYQLKKENERM"
     gene            4069..6057
                     /gene="uvrB"
                     /locus_tag="VQM60_001492"
     CDS             4069..6057
                     /gene="uvrB"
                     /locus_tag="VQM60_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET1081354.1"
                     /translation="MINRITDNKFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVSKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            6067..6630
                     /locus_tag="VQM60_001493"
     CDS             6067..6630
                     /locus_tag="VQM60_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081355.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNFGMMKLAEKLRMKKEAHIPKVRYYQGVY
                     YDSIKYGILREEWEEANGHHCQTDGNS"
     gene            6596..7084
                     /locus_tag="VQM60_001494"
     CDS             6596..7084
                     /locus_tag="VQM60_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081356.1"
                     /translation="MVITVKQMETPEEIEGKSLVHWKAWRESYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAMDGLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALDHFSEIFLWVLKDNKRAIAFYQKMGYTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            7157..7624
                     /locus_tag="VQM60_001495"
     CDS             7157..7624
                     /locus_tag="VQM60_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET1081357.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRSPDTNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWIQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKEIF"
     gene            complement(7621..>7828)
                     /locus_tag="VQM60_001496"
     CDS             complement(7621..>7828)
                     /locus_tag="VQM60_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HET1081358.1"
                     /translation="VYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGVHYHLV
                     EAETQQDLHYIEEALDQLGYLVKD"
BASE COUNT         2296 a         1444 c         1714 g         2374 t
ORIGIN      
        1 cgagggtgtt gtgggttatc aaagatttgg tcaggtgttc cgtcttcaag gaactcgcca
       61 tctgcagtaa agataacgcg gttggcaacc tgacgagcaa atcccatctc atgggttacg
      121 ataatcatgg tcatgccttg ctcagccaat tccttcataa cgttaagtac gtctccaacc
      181 atctcagggt caagggcaga agttggttca tcgaagagca tgatgtctgg attcattgct
      241 aggccacgag cgatggccac acgttgtttt tgaccacctg ataggctatc tggattggca
      301 ttagctttat ctgctagtcc aaccttttca agcaactcca ttcccaattc ctcagcttct
      361 tccttagtca tcaacttgtg ctcaatagga gcaaaggtga tgttgtccaa tacagacata
      421 tgagggaaga ggttgaagtg ttggaatacc atgccgatat tttcacggac gtggtcaaca
      481 ttggttgttt tttcagttaa atcatagccg ttcacagtga tgtgaccgct agtgacttct
      541 tctaaaagat tgaggctacg gaggaaagtt gacttaccag aacctgaagg accgatgata
      601 caaacaacat ctccttcata gaacttagtc gtaattcctt ttaggacttc attttttcca
      661 tagtgcttgt gtaaatcatt tacatcaatt tttaattttg ccattaacga atcctctttt
      721 ctaagcgttt cgctagtcta gtcaaaagtg tgataattac aagatagaag atagcaagga
      781 ttgcatacat cttgaaactt tggtagttac gagcaatgat aatcttacca gtttggaaga
      841 gttcaaccaa accgatagca gatacgatag ttgtatcttt aagagcgata acgaattggt
      901 tgacaaagtt tggcaacatc aatttagttg cttgtggcaa gataatctta cgcatggttt
      961 ttccataaga gatacccaag cttcggctgg cttccatttg gccaactgga acggcctgaa
     1021 taccaccacg aacgatttca gcgatataag cagccgcatt gagtgagagg gcaattgttc
     1081 cagctacaaa gtcgttaatt gggctttgtt ggcctgtgat agactcgatg aagtttggaa
     1141 ttccccagaa gatgaaggct gcaagaatca tcaatggaat accacgaata acgtcaacga
     1201 aaatctcaga gatgacgcga agagatttgt atgggctaac gctaaacata ccgaagataa
     1261 ttccgatgac aatggcaata gcaaatgaga taagagctag agcaagagtg ataccaagac
     1321 cgctaaggag ttgtttgtag ttgttttgaa gcaagcccca gagcgttgtt tcgtcaacag
     1381 tacttgttga agcagttgaa gattcgctag ctaggtattt gtcaagaatc ttttggaatt
     1441 caccgtttgc tttaaggttt gcaagtccgt tgttgaacat ttcaatcagt tctggatttg
     1501 ctcctttttt aacggcaaag gctgtttcac cgattggagt tccagagatt ggagttttca
     1561 atttttgacc ttggctgata gaatatttga gaacaggttc atcatccata acggcatcaa
     1621 tggcaccagt gtttaaactg tcatacattg aagaaccatc agcaaaggtt ttgattttgt
     1681 aaccgtattt gctttgattt tctgttagga aggtttgaga agcagttccg tttttaacac
     1741 cgactgtctt tccttttaga tcttcataag aagcaatatt gcttgattct ttgacaccaa
     1801 gaatggtatt agcagtgtag tatgattctg agaagtcaaa agttgcctta cgagcatctg
     1861 tgacagacat accagcaatg ataccatcgg cttgaccagc ttggacagca ctgatagcag
     1921 catcaaaacc agggttggtg atttcaattt caaaaccttg gtctttagcg attgccttaa
     1981 tcaattccat atcaatacca gtgtattggt tgcttgaatt ttggaaaaca aaaggtgcaa
     2041 aagaagaatc gctggcaatg atatatttag ccttaacagg aatagctttt aatcctgcga
     2101 gagtggttgt agttggcact gctgaagatg atgaagcagt ccatttcttg ataattttat
     2161 caagactacc atcttttttc atttcagcca aggcttggtt aaattcagta accaggtgtt
     2221 cgtatttact tccttttttc acaccgaaag caaaacttcc tacagcttca ccatccattt
     2281 caatatggag gtcttgacct tggttaatgg catattcgat aacaggtttg tcatccatca
     2341 tggcatcgat ggcaccagca ctcaagctgt tgttcattaa atccccagtg tcaaatgttt
     2401 taatagtaaa gccgtattta tctttgattg tttcaaggaa acgttgagcg gcagttccgt
     2461 ttttaacacc aacggttttg ccagttaatt ggtcgtactt gctaattttg tgtgactttg
     2521 tagtagcaat gacaactttt gtatcatagt aagtatcaga catggtgaag actttttcac
     2581 gttctttagt ctttgtcatc cctgccatga tagcgtcggc ttgcccagct tgaaccgcat
     2641 tgactgctgc gtcaaatcca ggataggaca tctgaatgtt ccagccttta atctcagcga
     2701 ctttgttaat aatgtcaaca tcaattcctt tataagtttg atctgaatct ttaaactcaa
     2761 aaggtgcata ggcggtatca gaaacaatct taatcgtttc tgctttggca atacctaatg
     2821 agaaaattgg gaataaaatt agcaaaaatg ctagaaattt tttcttcatt tttagtctcc
     2881 ttttccgaat attctctcat tatatcagaa aaagtaaaaa aaggcaaatt tttatatttt
     2941 tcgtgtcaga aaatataaca ttgatttttt cctttccttg aattgctatt aaaaagtgct
     3001 ataataatag tatatataga agaaaagagg acggggatat gaagaacaaa agaatattta
     3061 aagacttcca agcttcaaaa atgagtttaa acatttacac aagccccttg ttagcctttg
     3121 tttttgtctt cataggagag tttgtggctt ttactttgta tggtattggc ttgttagctc
     3181 tcatcggact tgctagaaat tttggagagg ctggtcaaaa tcttgcaagc tacttgcaga
     3241 ccttgcatca gagcttgacg gataaaacaa gtgactttcg tttaatttta ggattactgg
     3301 cctttggttt tattcttaac actgtgttca gatggacaag aaaagttgag aaaagaccta
     3361 ttcgaacctt gggattttat agagagaatt tcctcagcaa tcttctgaaa ggatttagtc
     3421 taggtctggc actttttctt ctgaccttgt taggtttggt ggccttaggg caatatcgtt
     3481 tggactccat tcacttggat acttattcgc tagcttttac tttatttaca ataccttttt
     3541 ggattttaca aggaacggca gaagaattgg ttactcgtgc ttggctgatt ccacaattgg
     3601 ctaagagaac caatcttaaa gtcgctatta tcatatctag tagtctcttt accttacttc
     3661 atttaggaaa tccaggcata actttcttat ctgcaattga tctcttttta tttggggttg
     3721 ccatgtccct atatcttctt aaaacagata ctatatgggg tataggtggt attcatggag
     3781 cttggaattt cgcacagggc aacctctttg gagtattagt tagtggtcag tcttcaggaa
     3841 cctcaatcat gaaatttact ccacaaggca atcaagactg gctatctggt gggtcttttg
     3901 gtattgaggg ttccatcgtg tccagtatta ttttgtttct gctgattctt tatctcgctt
     3961 accaattaaa gaaagaaaat gaaaggatgt gacttcggtt cgtccttttc ttcgtgaaaa
     4021 tgatacaagt atgctaaaat aggaatagca catggagaga ggattcttat gattaaccgc
     4081 attacagata ataaatttaa actagtatca aaatatcaac catcaggaga tcaaccccaa
     4141 gctatcgagc agttggtaga taatatcgag ggtggagaaa aagctcagat tctgatgggg
     4201 gcgaccggaa cagggaagac ctataccatg agtcaggtca tttccaaagt caataaaccc
     4261 actctagtca ttgcccacaa taaaactctg gctggtcagc tctatgggga gttcaaggaa
     4321 tttttccctg aaaatgcagt tgagtacttc gtatcctact atgactatta ccagccagaa
     4381 gcctatgtcc cttctagtga tacctatatt gagaaggata gttctgtcaa tgacgagatt
     4441 gacaagcttc gtcactcagc gacttcagcc ctattggagc gcaatgatgt tattgtcgtg
     4501 gcctcggtct cttgtatcta tggtctgggt tcgcccaagg aatacgctga tagtgtcgtt
     4561 agtctccgtc caggtcttga aatttcccgt gataaactct tgaatgactt ggtggatatc
     4621 caatttgagc gaaatgatat tgatttccaa cggggaagat ttcgcgttcg tggggatgtg
     4681 gtggagattt tccctgcttc ccgtgatgaa cacgcttttc gagtagaatt ttttggagac
     4741 gaaattgacc gtattcgtga ggttgaagct ttgacaggtc aagtccttgg agaagtggat
     4801 catttagcga ttttccctgc gactcacttt gtgaccaatg acgaccacat ggaagttgct
     4861 attgccaaga ttcaagcaga attagaagag caactagctg tctttgaaaa ggaaggcaaa
     4921 ctgcttgaag cccagcgctt gaaacaacgg acagagtacg atatcgaaat gctgcgtgag
     4981 atgggctata ccaacggggt tgagaactat tcacgtcaca tggatggacg gagcgaagga
     5041 gagcctcctt acacacttct tgatttcttt ccagatgatt tcttaatcat gattgacgag
     5101 agccacatga ccatgggaca aatcaagggc atgtacaatg gagaccgttc tcgtaaggaa
     5161 atgttagtaa attacggttt ccgtttgccg tctgctctgg acaatcgtcc tctccgtcgg
     5221 gaggagtttg aaagccatgt gcaccagatt gtttacgttt cagcgacacc tggtgactat
     5281 gaaaatgaac agactgagac agtgattgag caaatcattc gtccaacggg actcttggat
     5341 ccagaggtgg aagtccgtcc gactatggga cagattgatg acctcttagg tgaaattaat
     5401 gctcgtgttg agaagaacga acgaaccttt atcaccacgt tgaccaagaa aatggcagaa
     5461 gacttgaccg actacttcaa ggaaatgggt atcaaggtca agtacatgca ctcggatatc
     5521 aagacattgg agcggactga gattatccgt gacctgcgct tgggtgtctt tgatgtcttg
     5581 gtcggaatca acctccttcg tgaagggatt gacgttcctg aagtgagcct cgtagcgatt
     5641 cttgatgctg acaaggaagg tttccttcgt aacgaacgtg gactcatcca gaccattgga
     5701 cgtgccgccc gtaacagtga aggtcatgtt atcatgtatg cggacacggt tacccagtct
     5761 atgcaacgtg ctatcgatga aactgcccgc cgtcgcaaaa tccagatggc ctataatgaa
     5821 gagcatggta tcgtaccaca gacaatcaag aaagaaatcc gtgacctgat tgctgtgacc
     5881 aaggcagttt ctaaggaaga agacaaggaa gttgatatca atagcctcaa caaacaagag
     5941 cgcaaagaac ttgttaagaa actcgaaaaa caaatgcaag aagccgtcga agtgcttgac
     6001 tttgaactag cagctcagat tcgtgatatg atgctggaag tgaaggcgtt ggattagggg
     6061 aatagtatga tttatttaag aaagttaaag aaagaagatt tgatgtcttt atgggaaatg
     6121 gcttattcac aacttaatcc agtttggaaa cagtatgatg ctccctatta tgatgattat
     6181 cagtattttt caaattttaa agaattcgaa ctacaaaaat cagaatccat tttaagcaac
     6241 tcaaatcgcc ttggtatttt tgttgatgat aaactagttg ggactgtttc gcgttattgg
     6301 gtatgtaaac aaacaagatg gatggaattg ggaattggta tttatgataa aaaattctgg
     6361 aacactggta ttgggaaagt tgctatgttg cagtggatag ataggacgtt tcaggattac
     6421 ttggagttgg agcatctggg tttgacaact tggtcaggaa attttggtat gatgaaattg
     6481 gctgaaaaat taagaatgaa aaaagaagct catattccaa aagttcgtta ttatcaaggt
     6541 gtttattatg atagtattaa atacggcatt ttgagagaag aatgggagga agcaaatggt
     6601 catcactgtc aaacagatgg aaactcctga agagatagaa ggtaaatccc ttgttcactg
     6661 gaaagcgtgg agagaatctt atgacgacct tttgcctgca gaatttcaag agacgatgac
     6721 attagaaagg tgtcgactct ttagtcaaaa gtatccagaa aatacattga ttgcgatgga
     6781 tggcctgaag gtagttggtt ttatcagtta tggcaacttt cgtgatgaga ctattcaagc
     6841 tggtgaaatt attgctttat atgttttaaa agactattac ggaaaaggca tcgcacaaaa
     6901 gttaatgaaa gcagctttga ctgctcttga tcatttttct gaaattttct tatgggtatt
     6961 gaaagataat aagcgcgcca ttgctttcta tcaaaaaatg ggttatactt ttgatggaca
     7021 agaaaaaata cttgaacttg gaaagcctat aaaggaaaaa cggatggtat tctattctaa
     7081 ataattttaa aaagtagaag ctcatggcac tggtaccaag tctaaaaatt taataactta
     7141 gaaagcgagt aaatttatgt cccgttccca attaacaatt ttaacaaata tctgtctgat
     7201 tgaagacctc gaaactcagc gcgtagtcat gcagtatcgc tctcctgaca ccaatcgctg
     7261 gtcaggttat gcctttccag gaggtcatgt agaaaatggc gaatctttcg cagagtctgt
     7321 cattcgtgaa atctatgaag aaacagggtt gactatccaa aatcctcaac ttgtcggcat
     7381 taaaaattgg ccactagata caggtgggcg ctatattgtc gtttgttata aggcgactga
     7441 gttctctggt acccttcgct cttcagatga aggagaggtt tcttggattc aaaaagacca
     7501 gattccaaac ttggatctgg cctatgatat gctacctttg atggagatga tggaagctcc
     7561 ggacaaatct gaatttttct accctcgccg tacagaagac gattgggaga aagaaatttt
     7621 ttagtctttt actaaataac ctagctgatc caaggcctcc tcgatatagt ggaggtcttg
     7681 ttgtgtttcg gcttcgacta ggtggtaatg gacgccatct gtcaattcag atagaggtct
     7741 aaagtcagaa tgctcgactt gttctagaaa atgttgcaca tctctgcgac aagacagttt
     7801 aagcaaggtt tcaatttctc cataaacg
//
LOCUS       DAVAAL010000086         7731 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVAAL010000086 DAVAAL010000000
VERSION     DAVAAL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.087"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(60..1472)
                     /gene="sufB"
                     /locus_tag="VQM60_001497"
     CDS             complement(60..1472)
                     /gene="sufB"
                     /locus_tag="VQM60_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET1081359.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     VKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(1526..1966)
                     /locus_tag="VQM60_001498"
     CDS             complement(1526..1966)
                     /locus_tag="VQM60_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET1081360.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(1953..3179)
                     /locus_tag="VQM60_001499"
     CDS             complement(1953..3179)
                     /locus_tag="VQM60_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET1081361.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(3190..4452)
                     /gene="sufD"
                     /locus_tag="VQM60_001500"
     CDS             complement(3190..4452)
                     /gene="sufD"
                     /locus_tag="VQM60_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET1081362.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(4480..5250)
                     /gene="sufC"
                     /locus_tag="VQM60_001501"
     CDS             complement(4480..5250)
                     /gene="sufC"
                     /locus_tag="VQM60_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET1081363.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(5400..5594)
                     /locus_tag="VQM60_001502"
     CDS             complement(5400..5594)
                     /locus_tag="VQM60_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET1081364.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(5622..7277)
                     /gene="dnaX"
                     /locus_tag="VQM60_001503"
     CDS             complement(5622..7277)
                     /gene="dnaX"
                     /locus_tag="VQM60_001503"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET1081365.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTTISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTNLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKSEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(7277..7714)
                     /locus_tag="VQM60_001504"
     CDS             complement(7277..7714)
                     /locus_tag="VQM60_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004234289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET1081366.1"
                     /translation="MEGETNVLANLSNASALIKSRFPNTVFAGFYLFDGKELVLGPFQ
                     GGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYISCDSLAKSEIVVPMMKNGQLL
                     GVLDLDSSEIEDYDTMDRDYLEQFVAILLEKTAWDFTMFEEKS"
BASE COUNT         2208 a         1774 c         1449 g         2300 t
ORIGIN      
        1 tacggtaagg cgatgctgac gtggtttgaa gagattttcg aagagtataa aatcaaattt
       61 tatccaactg atccctccat ttcatagcta atcaagcggt tcagctcaac tgcgtattcc
      121 attggaagtt cttttgtaaa gggttctaca aatcccatga caatcatttc agttgcctca
      181 gattctgaca atccacggct catgagataa tacaattgct cttctgaaat cttagatact
      241 ttggcttcgt gttccaaagc cacttgcgag ttgtgaattt cattaaatgg aatagtatct
      301 gatgctgaca agtcatccat gataatggta tcacattcaa tgtgggaaac agatttctta
      361 gagttcttgt taaaggtgac ttgtccacgg tagtcaacct ttcctccacc tttagcgatg
      421 gatttagaca caatagacga gctggtatgt ggagcattgt gaatcatctt agcacccgtg
      481 tcttggtgtt gccctgcatt agcaaaggcg atagagagca tggtaccacg cgctccttct
      541 ccatcaaggt aaacagatgg atatttcata gtcgttttgg cacccaagtt tccatcaatc
      601 cactcaacag tggcatcctt ttgagcctta gcacgctttg ttaccaagtt atagacgtta
      661 tcagaccagt tttggatagt tgtataacgc atataagctc cgtccaaagc aaaaatttct
      721 acaatggcag cgtgcaagct attgcttgaa tatgttggtg ctgtacatcc ttctacgtag
      781 tggacgcttg ctccctcatc aacgataatc aaggtacgtt cgaactgacc tatattttcg
      841 ttattgatac ggaaataagt ttgaagtgga atatctacct tgacaccttt tggcacgtag
      901 ataaaagttc cacccgacca tactgctgag ttgagggctg ccaacttgtt atctgtcggc
      961 ggtaccaact tcgcaaagta ttgtttaaat aagtctgggt attccttgag tgcggaatct
     1021 gtatctgtaa agataatacc taatttttgg aactcttcct tcatgttgtg gtaaaccact
     1081 tctgactcgt actgggcaga agcccctgct aaataagcac gttcagcttc tggaatcccg
     1141 atacgttcaa aggtttcttt aatcttttca ggtacatcat cccaagaacg ggctggtttg
     1201 tcagatggtt tttggtagta gattaagtca tcaaagtcaa tctctgacaa gtctgctccc
     1261 caagtttgca tgggcatttt tttgaaggtt tcataagact tcaaacggaa ctccaacatc
     1321 cactcaggct cacccttaac agcagataat tcacgaataa caccttcgtt gagtcctttt
     1381 cctgtcgata agacaggctc tacatcgtca tggaaaccaa atttatattc accaaggtca
     1441 attggttttg gttctactct ttcttcagcc atagtatcct ttctttcttc atttctaata
     1501 attcataaga caaaagaaac ttgtcttact gtttttcttg attttcaatt gttttcttaa
     1561 gggcattcca agctagggtt gcacacttga ttctttgagg gaatttggca acacctgaca
     1621 agaatgccgc gtctccaagt tggccttgac gctcatcttt ttgcccttga accatttcag
     1681 aaaaaatagt cgccagttct aaaatttctt gtttggtttt tcctaaaacg gcatctgtca
     1741 tcatactagc agaagcagtt gaaatcgtgc atcctgaatt tagaaaagca atatcttcca
     1801 aacggtcctc tgcatcaaac ttgacagaga ggttgatgac atccccacaa gtcggattgt
     1861 tgagactgat ttgctcagca tcttctaact tcccttggtg atgtggattt ttcgaatggt
     1921 ctgctaccac tgccatataa aggctatcta gtttagaaag tgccattgaa aaactccttt
     1981 gtcttttgta gggcatcgac tagtttgtcg caatctgcct tggtattgta gatataaaaa
     2041 cttgcacgag ctgttgctgg gacttccaaa tactgaagca agggttgcgc acaatggtga
     2101 ccagcacgaa cagccactcc ttcataatcc agagccgtcg caagatcgtg aggatggaga
     2161 tcacctaggt taaaggcaat aacacccgaa cgttgagcca aatcctgaga accgtaaatg
     2221 gtcaatccct caattgcctg cagttttgga tagacgtacg caatcaattc ctgttcatga
     2281 gcttcaacgg catccatacc aatcttttcc agataatcaa ctgcagtcgc aagtccaata
     2341 gctcctgcca tatttggcgt tccagcctca aatttccaag gcaattcctt ccaactagca
     2401 aattgctcgt agacaaaatc aatcatctcg ccgccaaatt ctactggaga catttgctca
     2461 agatactttt ctttgccgta aaggacaccg ataccagtcg gaccagccat cttgtgaccc
     2521 gaaaaggcga aaaagtccag atccaagtcc tggacatcaa tcttcatatg aggtgtagat
     2581 tgagcaccat ccactaccat aattgcccca acttggtggg ctaattgagt gatttccttg
     2641 atcggattga ccacaccaag aacattggag gcatgagcta gggaaacaaa tttaacctta
     2701 tcagtcaatt tagctcgcaa atcctccata tccaaggcac cgtctttaag atagacatag
     2761 acaagctctg ctccagtctt tcgacaagct tcctgccatg gaatgatatt agaatggtgt
     2821 tccattactg aaatcaagac ctggtctccc tcagcgagaa tttcctcagc aaagcgtgcc
     2881 acccagttaa ggctggttgt cgttcctctg gtaaagagaa cttcctttgt agagcctgca
     2941 ttaataaact tacgaatggt ttcacgagca gcttcataag aagctgtcgc tcgttccgct
     3001 aaggtatgga caccacggtg aacattggca ttgtcctgct catagtagct gttaatagct
     3061 ttcagaacta ctagtggttt ttgtgtcgtc gcagcattgt ccagatagac cagaggttca
     3121 tcattgacaa tctgatctaa aattggaaaa tccttgcgaa tcgcttctac atctaacata
     3181 ggcttcccct tagcgttttg acaatttctc ttcgatagtt gcaatcattt catcacgaac
     3241 ttccttgact ggaatctcca cgataacaga tccaaggaaa ccacgaacaa ccaaacgctc
     3301 tgcagttgcc ttatccaatc cacgactcat gaggtaatac atgtcttctg gatctacctg
     3361 accaatagag gctgcatgtc ctgcagttac gtcattttca tcaatcaaaa gaattgggtt
     3421 agcatctgaa cgcgcttggt ctgaaagcat gagaacacgg ctctcttgtt gcgcatctgc
     3481 tcccttagca cccttgatga tgtggccgat accattgaaa gtcaaagttg ctttttcaag
     3541 gataacccca tgttgtagaa tgtttccgat tgagttgcag ccatagttag ttacacgagt
     3601 atcaatccct tgtacctgac gaccacttga aagagctaca accttgaggt cagcatggct
     3661 accattacca atcaagtcac tatcaaaatc agcaacgaca tttccttcgt tcatgacacc
     3721 gatagcccag tcaatacttg catcgttgcc taatttacca cgacggctaa tgtaggcagt
     3781 gacgttttca cctagacggt cgatagcagc aaacttgact tgcgcaccag aacgtgcaat
     3841 cacttccact gtgatattgg cagttgcttt gtcacttcct tcaccgcgtg actctaaacg
     3901 ctccagataa ctaatcttag aatttttacc agcgataatc ataatatgct tgttaaacgg
     3961 cacattgcta tcgctatctt ggtagaaaat tccttcaatt ggctctgtaa tttctacgtt
     4021 atctggaata tagagtacag caccactgtt aaagtaagct gtgtggtaag ccgccaactt
     4081 gtcatcatca tacttaacag atgacatgaa gaattcttcg atcagctctg gaatttcttc
     4141 taaagctgag tgaaagtctg tgaagacaac accctgttca gctaactcaa ctggagtttg
     4201 ttcgaaaaca gtttgagttc ctacttgcac caacttcaag tgatgatcta aagctgtgaa
     4261 atctggaaca tttgctgatg gctcattttc tgtaatcgtt ccatcaccca gattccaacg
     4321 gtggaatttg acacgctcaa taactggtaa ttccaaagtc tcaatcttgt caaaagcttt
     4381 ttgacggaga tcagccaacc agcttggttc agcgtgcatt tctgaaaaaa gtttaatatt
     4441 ttctctagtc atttacttct cctaaaagat acgagggaat tacaattctt ccttgtagtc
     4501 gtagccaagt tcttcagcta attttgcgta tccttcacgt tccaaacgcg cagccaattc
     4561 tggaccacca gaaaggacaa cacgaccttc catcatcacg tgtaccacat caggtgtgat
     4621 atagttcaaa agacgttggt agtgagtgat gatcatagca ccaaaacctt caccacgcat
     4681 ggcattgaca cctttagaca caactttaag agcgtcaata tcaagacctg agtcaatctc
     4741 gtccaaaaga gcaaatgttg gctccaacat caaaagttga agaatttcat tgcgtttttt
     4801 ctcaccacca gagaagcctt cgttgaggta acgctctgcc atttcttctt tcatgttgag
     4861 caattccatt ttttcatcta gcttagtaat aaactcacga actgaaatct tctcatcatc
     4921 ttctttaccc gcattcatag cggcacgaag aaactcagca ttggtaattc cagggatttc
     4981 tgatgggtat tgcatagcaa ggaaaagtcc catacgcgca cgctcatcca cttccaactc
     5041 aaggatgttt acgccatcaa acaaaacttc acctttagtt acttcatagt ttggatttcc
     5101 catgatagcg gcagaaagag tcgatttacc tgtaccattt ggtcccatga tagcggcaat
     5161 ttctcctgtt ttcagggtca ggttaacccc ttttaaaatt tcttttcctt caatctcaac
     5221 gtgaagatct ttgatctcta ttactgacat gatagttcct ttctttttca aaacttttac
     5281 agtacttact agaaaaacgt ctgatttccc cagaaataga gcaaaaattc tataaatata
     5341 cttttctagt ataacaaaaa aaagcctaaa aggctttgat tttgtctgga agctgagggc
     5401 tagtctttcc tattaaaatg ctgatcgatg acatcaacga ttttgcccat cacataactg
     5461 actcggaaat ttctaaatag taaaattgcc caaaaggctg ccataatata gcggccagtc
     5521 atccaggctt cattgaaaaa ataggtctca gcagctgtaa acagcgccat aataataaat
     5581 tgttgtttat tcataaactt attgtatcat gaaatctttc tttagtcttc ctctaccttc
     5641 actttatcag ccaaaaattc aaatccttct ggaatcaggc tttcttctac ttctttttca
     5701 gtttgagaag atttggcttt ggctgaaaag gctgcgcgaa cttctttcca ttcctccatg
     5761 gaaatagcta aaatctcagg tgaaaaacct gccgcctgac tgaggatatt accaaacatg
     5821 gtattgagat tgtctcgttt catagtttga ccagcattga agttagactc aaaagcaaga
     5881 atagcatggt gttcattggc agcaaccggt tgagaaccaa ctagcagagc cttgtccggc
     5941 ccacctagac tttcaattac ctctccccag gcattctgca aacgaattaa attttgacgt
     6001 gctaaatcag gattttcgac ggcctcttgt aagatagatt gcactttatt gcgatcgaca
     6061 cgatagactg ttttgcccgt agctggtcga ctaggagctg gtgcaacttg tttaggaacc
     6121 gcacctacat tagaaagctc ttgtttgaga cgggcaactt cctgtctcag cgtagcaatt
     6181 tcattttcaa ccgctcctga tagagctggt tcggacttga tttccgccaa acggacggtc
     6241 atcatttcag cataaatctt gggctgcaaa ctagacttaa tatctgctaa actcactgtt
     6301 gctaagcgaa tcatttcaaa cagatttttt tgaggaagtg ccaaattttc tacaaagact
     6361 gaactatgat gagtattttc tccccctgtt tgaacaatta acaagtctct taaatagtgc
     6421 aaaagattgg tcacaaaacg agtcatgctc ttaccattgt caaaaagaag attcaagcaa
     6481 gacaaagctt tgggaacatc ctgttgagac aaggccgcca cataatcatc caaggctgat
     6541 aggctaatgg tgccagtaat ttcttcagag atagtagtcg tcagctcatt tccctgtgtc
     6601 aaactcaggg cttgatccaa aatagacaag gcgtcccgca ttccaccttc cgcccgtctg
     6661 gcaatgattt ccacagcctc tggttcagaa ctgatatttt ctttttctaa gatatagtga
     6721 atatgttcct taatatcctg tgtcttaatt gatttaaact caaaacgttg cacacgggat
     6781 agaatagtag caggaatctt gtgcaattca gtagtggcca aaataaagac tacattctgt
     6841 gttggttctt ccagcgtctt taggagggca ttaaaagctc ctgtagacag catgtgaacc
     6901 tcatctatga tataaacctt ataacgagca aggctaggcg cataggtaga tttatcacga
     6961 atttcgcgaa tttcatctac cccattatta gaagctgcat ccatttcaat gacatcttct
     7021 aaactaccgt ccgtcactgc ttgacaaata tagcagttat tgcaaggttc gccacccact
     7081 tgattgggac agttcatagc cttggcaaag attttagcaa cactggtttt tcccgttcca
     7141 cgaggaccag aaaaaagata agcgtgactt attttctctt gctccaccgc ttgtttaaga
     7201 gtcttagcca caacttcttg accaactaac tgggagaagt tttgacttct atattttcga
     7261 taaagtgctt gatacattaa gatttttcct caaacatcgt aaagtcccat gctgtctttt
     7321 caagcaaaat agcgacaaat tgttccaaat aatctcgatc catagtatcg taatcctcaa
     7381 tctctgaaga atccagatcc agaactccaa gtaactgacc attcttcatc atcggcacca
     7441 caatttcact tttagctaga ctatcacaag aaatatagtt gagataggtc gtcacatctc
     7501 caacaataac agtttcctga aagtgagctg cctcaccaca aacacccttg cctagtgcaa
     7561 tacggatgca ggaaacacct ccttggaagg ggcctaaaac caattccttt ccatcgaaca
     7621 aataaaagcc tgcaaatacg gtattaggaa aacgtgattt tatgagagca ctggcgttgg
     7681 aaagattagc caaaacattg gtttcgcctt ccaataggaa ggacaattct t
//
LOCUS       DAVAAL010000087         7471 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVAAL010000087 DAVAAL010000000
VERSION     DAVAAL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.088"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(17..850)
                     /locus_tag="VQM60_001505"
     CDS             complement(17..850)
                     /locus_tag="VQM60_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET1081367.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(864..1730)
                     /locus_tag="VQM60_001506"
     CDS             complement(864..1730)
                     /locus_tag="VQM60_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET1081368.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(1744..3003)
                     /locus_tag="VQM60_001507"
     CDS             complement(1744..3003)
                     /locus_tag="VQM60_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET1081369.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(3095..5257)
                     /locus_tag="VQM60_001508"
     CDS             complement(3095..5257)
                     /locus_tag="VQM60_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET1081370.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            5365..6225
                     /locus_tag="VQM60_001509"
     CDS             5365..6225
                     /locus_tag="VQM60_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET1081371.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKKGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            6222..7157
                     /locus_tag="VQM60_001510"
     CDS             6222..7157
                     /locus_tag="VQM60_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET1081372.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLIQCDNGKEIWLNSGEISLNSWK"
     gene            complement(7283..7370)
                     /locus_tag="VQM60_001511"
     tRNA            complement(7283..7370)
                     /locus_tag="VQM60_001511"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7334..7336),aa:Ser,seq:gct)
     gene            complement(7377..7450)
                     /locus_tag="VQM60_001512"
     tRNA            complement(7377..7450)
                     /locus_tag="VQM60_001512"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7414..7416),aa:Ile,seq:gat)
BASE COUNT         2365 a         1578 c         1352 g         2176 t
ORIGIN      
        1 tgattttcat tgagtattac ttcactgccc cgttgctcat tcctgaaatg atatggcgtt
       61 ggaagaagag atagacaatg gtgatactga taatgccgac cacgtaagag gcaaagcttg
      121 gtccgtagtc gttgaaatat tggcctgcgt agttgtattg gaacaaaggc agagtccaca
      181 ttttggaatc ccggttcaag acaaggagtg gcaacatgaa gtcattccag aaccaaaggg
      241 cattgatgat catggttgtc gcatgcatcg gtttcatcat tgggaagatg atgcggaaat
      301 aggttgtaaa ttgattagcc ccatcgatct ctgctgcttc atccagactt tctggaatcg
      361 agattttgat atagccaaca tagagaaaga gggtctgtgg aatcgcatag gtcaagtaga
      421 gtaagatcaa accaaaggta ttagccaaac cgagtttact catcataacc gtaatcggaa
      481 tcatgatgac ttggaaaggt acgaagattc cgaggattaa gagggtatac atgatggtaa
      541 aggcttttct tttactcata ttgcgagcga tggagtaggc tgccataggt ataaagatca
      601 ttactgcaag taaagacaag acagtgatga cgacagagtt ccaataatag cctccaatcc
      661 catcagctaa gagacggcta aagttgtccc atgtgaagtt ggttggaaag ccaaagaaat
      721 tatctacaat atccttagtg ggtttgaagg aactaaagag ggtagcaagg agcggcacta
      781 aaatcagaac cgatcctaga atcaatagaa tgtatttgcc aatcagggct tttctttcat
      841 cttgtttcat catgcttctc ctcttaaatt tcaaatttct tagatactct caattggatg
      901 atcgaaatca ctacaattaa gaagaacaag attacggcaa tggcattggc ataaccgaat
      961 tggttgtttt taaaggcata gttataaacc aagagcccaa gtgaggttgt ggcattgttt
     1021 ggaccaccac cggtcatggc aaagacttgg tcaaaggcag tcagcccacc ttttagggct
     1081 aggataaaga ccatagagac acttggtagc aagtaaggca attcaatgtt ccagaaaact
     1141 tgcttgctcg tcgcaccatc aatccttgct gcctctgtaa tctcagttgg aatagattgc
     1201 aaaccagcta ggaagatgat gatgggcata gccacccctt gccaaagaag gacaaagaca
     1261 gccgcaaaga ttgctcccca cttagtccct aaaagactgg tttggagaaa ttcaatatga
     1321 agggcattcc caatcgctgg aagaccgtag ttgaagactt gcttgaagat caaagccact
     1381 gtcaaaccag ataaaacagc tgggaagaag aaccaagcac ggaagaaggt ttggcctttg
     1441 attttagaat tcaagacacg cgcaatgaag atcccgagtg caatctcacc aaccaccatg
     1501 gcaatcgtaa tgattgcggt aaagccaatc gcattcatga attttggatc catgaagagg
     1561 agcttaaagt tgtttaagcc gacaaatttg tagttataag tcaatcctgt ccagttggta
     1621 aaactgtaaa aggctccttg aaacatcggc acatagaaga aaattgcttg taacaagagg
     1681 gggatgacca caaaagccca tgcccaatat ttttgtaata cttttttcat agtctctcta
     1741 ctcctaatcc acatccgctt tcatcgggtt aaagaaggca ttcaaatcat tgaccatgcc
     1801 ttgtttatca ccggtcaaga catagttcat ggtcaaggta tggaagtctg cttcactggt
     1861 ccagtattgt tgcaaccaga ccaagtgacg atccgtaaag gcatattcgg tcataccagc
     1921 aagcggtgaa tcttctcctg cttgtttgac tccttcgatc gctgttggag atccgtccac
     1981 atcgtagtat ttttgcatga cttctggacg ggtcatatat tccacaaagg cattggcttc
     2041 ttttggatgt ttggtggtgg ctgagataga ccatgccaag tctcccgcac caacggttaa
     2101 gctttgtcct ttttcttttc ctggaatcat gaaggtccca atcttaaagt tcggtttttg
     2161 ttcattaatc gctgtgatcg cccaagaccc atttggtgtc atgaggacat ccccacgtgc
     2221 gaaggctccg ataacatcgg tatagccagc accttcccag ttcttttgct tagagccatt
     2281 gatgcgaagg atgtccatga ccttgatatc atctttcata atcggatccg acaatttaat
     2341 ggcatttggt tgagaataac gaaggtattg atttgcttct tttcctccac ctgttgctgt
     2401 cgcaaaggct aattgattgt aaccattgag tgtccaagca tctgcacctg caattccaaa
     2461 tggtgtttgt cctttagcaa cgatatcttt gactagctgt tcaaattcat cccaggtttc
     2521 aggaaccttc aagcccagtt cttcgaattt atctttgttg tagtaaattc cataagcatt
     2581 agctgtaaaa ggaacgttgt aaactttttc gtttacagca tatttttcag cgtagccatt
     2641 tttcacgcgt ttcaggtagt ctttgttgct caaatcttca aaaacacctg cttttgccca
     2701 ttcttgcagt tcgatggact gtgggtaaat attgaccaca tcaggcacat ctcctgcgag
     2761 aacgcgtgtc ttcaatactt caccagcatt tggtacattg acgactttga ccttgatctt
     2821 agggttttcc ttctcaaaat cacgagtgat ttcttccaag gttttggtca tttctttttt
     2881 ctggttgaaa tactcgatgg tcactgtgcc atccgcagat ttaccatagt tggagcaagc
     2941 gccgagccca aacaaagcta aacctgtagt tgcaagaagt ccgatttttt tataccattc
     3001 cattagaaag cctcctttat aaatttatac acccttattg aactgcaccc caaaagttag
     3061 acagaataaa tctaactttt ggggtcagta catatcatag ttttctaaaa atatactgtc
     3121 tactcaaaaa atctccttgg gataagataa cagttaagcc cgcatacatt agttctgcac
     3181 ctgagtaaac ttcgccattt tcctgtaatt tatatagtcc ctcttcatcc aaatctttta
     3241 attttaaagt tgtttccatg gtctctacaa cagataaaac gcgaacgtag gttacaatcg
     3301 tttgatttcc gtaattaaat tgtacagctg cttcattgga tacagtatca ggattaatta
     3361 gtctatactg ctgtcctaac tgaactactg gtcgtaattc tttatacaag ttcacctgat
     3421 tagcaatcgt agctttctct tcatctgata aatttgtcaa atcaagctca tagcccaaat
     3481 ttcccatcat tgctacatgg ccacgtgttt ctaatggtgt cattcgtccc atctgatgat
     3541 tcggtactgc tgacacatga gcccccatag aaatggttgg atagagatag gatgaaccgt
     3601 attgaattgg taaacgtgca atggcatcag tattatcact agcccagact tgtgggaaat
     3661 agcgtatcat accaagatca tttcgtccac caccaccaga gcaggactca aagagaatat
     3721 ggctgtgctt ctctgtcaga taagaaacga gttcgtaaag ccccagcatg tactgatgag
     3781 attgcatctg tgtctctaga taagttaatc cattccctag cttagtgata ttgcggttca
     3841 tatcccattt aatgtaatca atctcatgat aagataggag ttgatctaag acacttttca
     3901 agtattctac tacctgagga ttggcaagat taagtactaa ttgattccga gaataagtat
     3961 gctcataatc aggaacctga atagcccagt caggatgttg acgatacaaa tcactatcta
     4021 cagaaatcat ttcgggttct aaccaaagtc caaactgcaa acctctttca tggatagctg
     4081 aaatcagact ttctagactt ccacccagtt tttcctcatt aacaacccaa tcacctaaag
     4141 cacgattatc atcaaaacga ttgccaaacc aaccatcatc taatacaaaa agttcaatgc
     4201 caactttctt agcttcatct gctaactcta acagtttttc tctctgaaag tcaaagtaag
     4261 tagcttccca gttattgatt agaattggac gttctttttt agaaaattca cttagcataa
     4321 tgtgcttcag tacaaaattc tgactttcat gactaatacc agttaatccc tgatctgaat
     4381 gagtcactaa agctaccggt gtttcaaagt attcctcagg ggctaacttc caagaaaagt
     4441 tttctggatt aatgccaata gccacccgaa cttcattcaa ttgatttttt tgaacaaaag
     4501 cttcaaagtt gccactatac attagttgaa tagcaaacac attcccagca tcctctgtga
     4561 ctccttgttc gcatagtaga agagctggtg tttgagcatg accagaagca cctcggtttg
     4621 agctaatcga aaagattcct tgttctacct gttgacgtct aacagtcttt tcacgagcat
     4681 aagcaccctg cagagttact atttcgtaat ctgcagctgg aaaatcagcc ataaaagaaa
     4741 aatctttatg gatgacaact tcctgattac tattattatc taatttactg tagctagcaa
     4801 tagtcgcatc attattaaaa gtagtataat acaaagtcag actaagttga gccttagaat
     4861 cttctaacat taagacaaga gtctctgtat cgtccatgct atgtggagaa ggtaagccct
     4921 gtggaccatt ctgacctttt aaaatctttg cttctacaaa tcgaaagtct gttacttcag
     4981 ttacactatg ctgaacctgt atggttggtt tcctaaaatc tcctaagcca tgttgtccaa
     5041 aaatctgtcg ctgagtatct aaactaaagg ttcgattagt agccgttgga tttcctgaaa
     5101 aggcatggtc tcgttcataa acactattgg aacctttata gttcttaata gtctttccta
     5161 aatgtttcaa aagtaagtag ccatttcgat tttcaataat caaacttaga tttttactct
     5221 caacataaaa tagattattc tctatcctaa ctcccattta cttcacctca tcactttatt
     5281 gattatattt tatcacctga aatcgctttc cgaaatagaa aaatgtctca agaatatggt
     5341 aaaatgttag gtaggaggta gcacatgtta gttttttcag aataccagac tggaacaatc
     5401 gaccttgccc taagctttta tggatatgag gaatgcacac ctaattactc ttttggtcca
     5461 gccattcgtg atacatacgt tctacattac attactaaag gacaaggaaa attttattac
     5521 aagggtaaaa ttgttgattt aaaaaaagga gatttctttc tattaaaacc agaggaacta
     5581 accttttatc aagcagatag taaagaacct tgggcctact actggttagg aatcactgga
     5641 gggaaatccc ctgattattt tgctctttct caaatttctg atcaatccta tctcatccaa
     5701 tctgaaactt gtcataccca gactactgca aaactcatct cagacattgt ccgcttcgct
     5761 cagattacaa aatcaagtga attagctcaa ctccatatca tgggacaact tcatgaactg
     5821 atgtttcatc tgggaactat tgctcccaat cagaaaaaaa agaatatttc atcaacccac
     5881 caactctatc ttgaatgcaa acgattaatt gatagccact atcctcaatc acttacaatt
     5941 caagatttag caaaagaact atccgttcac agaagctact tatcaagcgt attcaaagaa
     6001 tttaatacct tatcacccaa agaataccta ctctacgttc gaatgcaccg agctagacaa
     6061 cttctcgaaa atacccaaga gtccatcaag gtaattgcat actcggtagg tttttcagat
     6121 ccactccatt tttcgaaagc ttataaacaa tactttaatc agactccaag tcatacaaga
     6181 aaagaatact ctcaatacca actagtaaga aaggcaacat tatgaaatcc taccaagctg
     6241 tctaccaaat cctatctaaa gaaaccgact atatcagcgg agaaaaaatc gcagaaaaac
     6301 tatccctaag ccgaacagca atttggaaag ccatcaagcg actagaacaa gaaggcattg
     6361 aaattgatag tatcaaaaat agaggatata aactgatgaa tggtgacctt attcttccag
     6421 agattctaga agaaaatctt ccaattaaag tcagctttaa acccgaaaca aaatcaacac
     6481 aactagatgc aaaagaagca attgatttag gccatgaagc aaataccctc tatctagctt
     6541 cctatcaaac agcaggccga ggccgttttc aacgttcctt ctactcacca caaggtggta
     6601 tttatatgac actccatctt aaaccaaatc tcccctatga caaattacca tcctacacac
     6661 tacttgtagc tggagctgtc tacaaagcta ttaagaacct aactttaata gatgtcgaca
     6721 taaaatgggt caatgatatc tatctaaaca atcataaaat tggaggaatc cttactgaag
     6781 caatgacctc tgtagaaact ggcttagtca cagatatcat tattggagta ggtatcaatt
     6841 tcactattaa agacttccct caggaattaa aagaaaaagc tgccagctta tttaaagcta
     6901 cagctcctat aacaaggaat gaattgatca tagaaatctg gcgtgctttc ttcgaaacac
     6961 cagcagaaga gctattatac ctatacaaaa aacagtcatt cattctagga aaagaagtca
     7021 ctttcacact agagcaaaaa gactacaagg gacttgctaa agacatctca gaaaatggaa
     7081 aacttttaat tcaatgtgat aacggaaaag aaatctggct aaatagtggc gaaatttctc
     7141 tcaatagttg gaagtaaaat aacacaatta taatataaac gatataaaaa taacttcaga
     7201 ttagtaattc aattaagttt tacggatctg aagttttatt gtctctaaaa acaaaaaaga
     7261 gagttacaaa ctctcattaa aacggagaat aagggattcg aacccttgcg ccagttaccc
     7321 gacctaacga tttagcaaac cgtcctcttc agcctcttga gtaattctcc aattaatggg
     7381 cacgagtgga ctcgaaccac cgacctcacg cttatcaggc gtgcgctcta accacctgag
     7441 ctacgcgccc aagttaaaaa acttggtaat t
//
LOCUS       DAVAAL010000088         7363 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVAAL010000088 DAVAAL010000000
VERSION     DAVAAL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.089"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..671
                     /locus_tag="VQM60_001513"
     CDS             <1..671
                     /locus_tag="VQM60_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET1081373.1"
                     /translation="SEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPKHIDEE
                     AVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKNAVVIG
                     RSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTADFVKPG
                     AVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQAALRT
                     LDRK"
     gene            813..1685
                     /locus_tag="VQM60_001514"
     CDS             813..1685
                     /locus_tag="VQM60_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET1081374.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENILALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERARKDKLV"
     gene            1967..2650
                     /gene="rpiA"
                     /locus_tag="VQM60_001515"
     CDS             1967..2650
                     /gene="rpiA"
                     /locus_tag="VQM60_001515"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET1081375.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            2664..3875
                     /locus_tag="VQM60_001516"
     CDS             2664..3875
                     /locus_tag="VQM60_001516"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET1081376.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            3877..4419
                     /locus_tag="VQM60_001517"
     CDS             3877..4419
                     /locus_tag="VQM60_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET1081377.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLGTFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            4435..5244
                     /locus_tag="VQM60_001518"
     CDS             4435..5244
                     /locus_tag="VQM60_001518"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET1081378.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            5720..6115
                     /locus_tag="VQM60_001519"
                     /pseudo
     CDS             5720..6115
                     /locus_tag="VQM60_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
     gene            6125..6699
                     /locus_tag="VQM60_001520"
                     /pseudo
     CDS             6125..6699
                     /locus_tag="VQM60_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            6724..7236
                     /locus_tag="VQM60_001521"
     CDS             6724..7236
                     /locus_tag="VQM60_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET1081379.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNGETAI
                     QVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         2264 a         1309 c         1707 g         2083 t
ORIGIN      
        1 gtagcgaagt agtgcgagtt ccagagacca ttactcaaga ggaattgtta gacctgattg
       61 ctaaatacaa tcaggatcca gcttggcatg ggattttggt tcagttgcca ttaccaaaac
      121 acattgatga agaggcggtt ctattggcta ttgacccaga aaaggatgtg gatggtttcc
      181 atcctctaaa tatgggacgc ctttggtctg gacatccagt catgattcca tcaacacctg
      241 caggaattat ggaaatgttt catgaatatg ggattgaatt agaaggtaaa aatgcggttg
      301 tcatcggtcg ttctaatatc gttggaaaac ccatggctca gcttcttttg gccaagaatg
      361 caacagtgac tttgacccac tcacgcaccc ataatcttgc taaggtggct gctaaagcag
      421 atattctggt ggtcgcaatc ggtcgtgcca agtttgtgac tgctgacttt gtcaaaccag
      481 gtgcggtagt cattgacgtt gggatgaatc gtgatgaaaa tggtaagctc tgtggggatg
      541 tggattatga ggcagttgcc ccacttgcta gctatattac gccagtccct ggaggtgtcg
      601 gccctatgac cattactatg ctgatggagc aaacttatca agcagcactt cggacattgg
      661 atagaaaata agagaaacat tcgattaata gagtgtattt tctatagcta tatctaaaat
      721 ggtagaaatg aatattaaat tttagatata agattacaaa aggaggtctg cgtctccttt
      781 ttattgtata ataaaagtga gaggaaaaaa ggatgaaagt gattaatcaa accttattag
      841 aaaaagttat tattgaacgt tctcgttcaa gtcataaagg agattatggt cgtctgctgc
      901 tgcttggtgg tacttatcct tatggaggtg ccatcatcat ggctgcttta gcagctgtaa
      961 aaagcggtgc aggattggtg accgttggaa cggacaggga aaatatcctg gctctgcaca
     1021 gccatttacc tgagcctatg gccttttctc ttcaagacca gcaattgtta aaagagcaat
     1081 tggagaaggc agaagttgtc ttgctggggc ctggtttacg agacgatgct tctggagaaa
     1141 atctagtaaa acaggtcttt gttaatttaa gccaaaatca gattttgatt gtagacggag
     1201 gtgccttgac cattcttgct aggacaagtt tgtcatttcc ctcaagccag cttatcctaa
     1261 ctcctcacca aaaagaatgg gaaaaattat ctggaattac gattgaaaag caaaaggaag
     1321 atgcaacggc tagtgttctg acttccttcc ctcaaggaac aattttggtg gagaaaggcc
     1381 cagctactcg tatttgggaa gttggccaat ctgattatta ccagttacag gttggtggtc
     1441 cttatcaggc gactggggga atgggagata ctctggctgg gatgatcgca ggatttgtag
     1501 gccaattccg acaggccagt ctctacgagc gtgtggcagt agcgacccat cttcattcag
     1561 ccatagccca agaactatct caagaaaatt atgtggtctt accgacggaa atcagcaggt
     1621 atcttcccaa aataatgaaa attatttgtc aacaagagag ggcaagaaaa gataaacttg
     1681 tttaaaatag acagataaat ctacttcctt ctcttaattt atgattcttg aaatgcgcct
     1741 gctttaaggc taaactagac gtatcaaagt taagagtttg tttttgtaac tggaaagaga
     1801 tgaacaaatc aaatacataa aagataaact catttttatt tgtttggtaa aaataaaatg
     1861 catattttta aagaaagatt gatgaaaaat gtagatttga tgtttttact tgaataaaaa
     1921 gcggtttcat agtaaaataa gaactgagaa tgatggaggt aaatcggtgg aaaatctgaa
     1981 gaaaatggca ggtatcaagg ctgctgagtt cgtgagcgat ggaatggtcg ttggacttgg
     2041 aacaggctcg actgcctatt attttgtcga agaaatcggt cgtcgtatca aggaagaagg
     2101 attgcagatt acagctgtaa caacttctag tgtgaccact aaacaggcag aaggccttaa
     2161 tatcccgctc aagtctattg accaagtaga ctttgtcgat gtgacggttg atggcgcgga
     2221 tgaagtggat agtcagttta atggaatcaa aggcggtggt ggtgcccttc tcatggaaaa
     2281 ggtggtcgca acaccatcaa aagaatacat ttgggtggtg gatgaaagca agctggtcga
     2341 aaaactaggt gcttttaaat tgccagtaga agtggttcag tatggtgcag agcaggtctt
     2401 tcgtcatttt gaacgagctg gctacaaacc aagtttccgt gaaaaagacg gccaacgttt
     2461 tgtgacggat atgcagaatt ttatcattga cctcgccttg gatgtcattg aaaatccaat
     2521 tgcttttgga caagaattgg atcatgtcgt tggtgttgtg gagcatggtt tattcaacca
     2581 aatggtggat aaggtaatcg ttgctggacg ggatggagtt cagatttcaa cttcaaaaaa
     2641 aggaaaatag aagggggcat aagatgtcta aatttaatcg tattcatttg gtggtactgg
     2701 attctgtagg aatcggtgca gcaccagatg ctaataactt tgtcaatgca ggggttccag
     2761 atggagcttc tgacacactg ggacatattt caaaaacagt tggtttgaat gtcccaaaca
     2821 tggctaaaat aggtcttgga aatattcctc gtgaaactcc tcttaagact gtagcagctg
     2881 aaagcaatcc aactggatat gcaacaaaat tagaggaagt atctcttggt aaggatacta
     2941 tgactggaca ctgggaaatc atgggactca acattactga gcctttcgat actttctgga
     3001 acggattccc agaagaaatc ctgacaaaaa tcgaagaatt ctcaggacgc aaggttattc
     3061 gtgaagccaa caaaccttat tcaggaacgg ctgttatcga tgattttgga ccacgtcaga
     3121 tggaaactgg agagttgatt atctatactt cagctgaccc tgttttgcag attgctgccc
     3181 acgaagacat tattcctttg gatgaattgt accgtatctg tgaatacgct cgttcgatta
     3241 cccttgagcg tcctgccctt cttggtcgca tcattgctcg cccttatgta ggtgaaccag
     3301 gtaacttcac tcgtacggca aaccgtcgtg acttggctgt atctccattt tccccaactg
     3361 ttttggataa attgaatgag gctggtatcg atacttatgc tgtgggtaaa atcaacgata
     3421 tctttaacgg tgctggtatc aaccatgaca tgggtcacaa caagtcaaat agtcatggaa
     3481 ttgatacact attgaagact atgggacttg ctgagtttga aaaaggattc tcattcacaa
     3541 acctagttga ctttgatgcc ctttacggcc atcgtcgtaa tgctcacggt taccgtgatt
     3601 gcttgcatga gtttgatgaa cgcttacctg aaattatcgc agctatgaga gagaatgacc
     3661 ttctcttgat tactgcggac catggaaatg acccaacgta tgcaggaacg gatcacactc
     3721 gggaatatat tccattgttg gcatatagcc ctgcctttaa aggaaatggt ctcattccag
     3781 taggacattt tgcagatatt tcagcgactg ttgccgataa ctttggtgtg gaaactgcta
     3841 tgattgggga aagtttctta gataaattgg tataatatga cgcgctatgc tttgctggtg
     3901 agaggtatca atgttggtgg taagaataag gtcgtcatgg cggagcttcg tcaagaattg
     3961 acaaacttgg gactggaaaa ggttgagagc tacatcaaca gtggcaatat tttctttact
     4021 tcgatagatt ccaaagccca attggttgaa aagctaggga ctttctttgc agtccattat
     4081 ccatttattc agagcttttc tttactgagt ctagaggact ttgaggcgga acttgaaaat
     4141 ctaccagctt ggtggagcag agacttggca cgaaaagatt ttctctttta cactgagggt
     4201 ttggatgtgg accaagtcat cgcgacagtt gaaagtttag agctgaaaga tgaagtgctt
     4261 tattttggaa aacttgggat tttctggggg aaattttctg aagaatccta ttctaagact
     4321 gcctatcaga agtacttgct gaaggtgcct ttctaccgcc acattactat tcgtaatgct
     4381 aaaacctttg acaaaattgg tcaaatgcta aaaaaataat aaaggagaca cacaatgaca
     4441 tttttaaaca aaatccatga aactgctact ttcctgaaag aaaagggaat tgcagcccct
     4501 gagttcggtc taatccttgg atcaggactt ggagaattgg cagaagaaat cgaaaatcca
     4561 gttgtagtag actatgctga gattccaaac tggggccgtt caacagtagt cggtcatgct
     4621 ggtaaattgg tatatggtga actggcaggt cgcaaggtct tggctcttca aggtcgtttc
     4681 catttctatg aagggaatcc tctggaagtg gtgactttcc cagttcgtgt gatgaaagtt
     4741 cttggatgtg aaggtgttat tgtaaccaat gcagctggcg gtatcagatt tggtcctggt
     4801 accttgatgg ctatctcaga ccatatcaac atgacggggc aaaatccatt gatgggtgaa
     4861 aacttggatg actttggccc acgtttccca gatatgtcta gggcctacac accagaatac
     4921 cgtgccactg cccatgaagt ggctaaaaaa cttaatatca agcttgatga aggtgtctat
     4981 atcggagtta ctggtccgac ttatgaaaca ccagcagaaa ttcgttccta taagacactg
     5041 ggagcagatg cagttggtat gtctacggtt cctgaagtta tcgtggcagc ccactctggc
     5101 ttgaaagttc tgggaatttc atgtatcact aactttgcgg ctggtttcca agaagaactc
     5161 aatcacgaag aagttgtaga agtgactgaa cgtgttaaag gtgatttcaa aggcttgctt
     5221 aaagcgattc ttgctgaatt gtaagaaaaa agatttaaaa ggggtagtgt ctctgtcttt
     5281 tcaggattga ctgcctatcc gtattaaaga agaaacagag gaatactatg agcttcttcc
     5341 tgctcttata actgaaagaa gcggaagaat aggtgtgtct gatctgatag ccagcattgt
     5401 gaaagacaag attctaggat actagcatta gcttcctagc caagcagact agtatgataa
     5461 ggagagatga gaatgaattg actttctgaa tttctcagtc ttatcatata tagcacaatg
     5521 agatttcgct tgagtctgct tgtaaataaa cgaaaagaaa gataagaaat aatgaaaatt
     5581 ggtcaacgaa ttatgcgctt tggcataaaa aattaagtat cggagttgta tctgttgtag
     5641 tcggctttga tttctagctc cagctggaat ttcagccaat gaagtaaagc aagatgtaac
     5701 atctgaagtg gtaataggtg tgctagattc taaggaggaa ttgaaagagt cagaaaatga
     5761 tgctccaaaa ctagaaactc ctcttagaga ggagccaaga ctagctcctc aaacgcttcc
     5821 ggaagcaagt gaagttcttg aaaacaaaag ggaagagtca aaagtagaga taacagagcc
     5881 agctcaagcg gatgatatcc gcaaggttgt tggggaatta gccaaggata taagtattac
     5941 taagttgtat atgacaggtc attctcttgg aggctaccta gctcagattg cagcggttga
     6001 agattactaa aaatatcctg atttttataa ccatgtattg aggaaagtga caactttcag
     6061 tgctcctaaa gtgattactt ccagaactgt ttgggatgct aagaatggtt tctgagatgt
     6121 tggtttggaa agtcgtaaat tagctgttag tggaaaaatt aagcattatg tggttgataa
     6181 tgacaatgtt gtgactccct tgattcataa taatcgtgat attgttacat ttacaggtaa
     6241 ttcacgcttt aaacaccgtt ctcgtggcta ttttgaaagt ccaatgaatg atattcctaa
     6301 ctttaatatt ggtaaacaag ctaccttgga taaacatggt tatcgtgatc cgaaattgga
     6361 taaagcgcga ttctttaaga aacaggctct acctcaatct tctagtcaac caagcgctga
     6421 accaatggaa aatattgcct taggaaaaca ggttactcaa agttcgacag ctttcggagg
     6481 aatgctagaa gagctgtgga tggcaaagtc gatggtaact atggtcacaa ttctgtcact
     6541 catacaaact tccaatctaa gccttggtgg caagtagatt tggctaaaga agaaaccatt
     6601 cgccaaatca atatttacaa ccgaacagac actgcccagg atagattggc aaactttgat
     6661 gtcattcttt tagacggttc tggtaaagaa attgagtgaa aacgtataat atctcctaaa
     6721 gatgtgtcag cacaaattac gattaaccat aaaaaagcgc gctatgttcg gattgagcta
     6781 gaaggctata atgccctcag tcttgcagaa gttgaagttt tccgctttat agctacgaat
     6841 ggtgaaacgg cgatacaagt ttctaagtca gttcaaccaa tcagtcagac tcctgtgaaa
     6901 gataaaacat tgacaattca acacagtgga gcttacattg cccgctactc cataacttgg
     6961 gaagaagtta cagtagataa agatggaaac caagttgttc gtagtcattc ttgggaagga
     7021 aacggtcgca accagactgc aggttttgtc ctcaacctcc caatcaaaga aaatatgaga
     7081 aatctgcgag ttaagattga gaaaaagacg ggcctactat ggaatagatg gcaaacaatc
     7141 tatgaaaaca gaccaatttt agctcaaccc caccgtaaaa ttacccattg gggtacgaca
     7201 ttgaattcca aggtgagtga cgatgatgtc ttgtaatctg atggtacaat gacagttagt
     7261 ttgtctagtt tataagaaag tactacctga gcttgaatag aactcaggta gctctctgtg
     7321 aaagaacaaa attaatactc aatgaaaatc aaagagcaaa cta
//
LOCUS       DAVAAL010000089         7194 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVAAL010000089 DAVAAL010000000
VERSION     DAVAAL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.090"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..227)
                     /locus_tag="VQM60_001522"
     CDS             complement(<1..227)
                     /locus_tag="VQM60_001522"
                     /inference="COORDINATES: protein motif:HMM:NF020296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET1081380.1"
                     /translation="MIDWDFADTCWEKDPRKYQYVAANYLKAMQSYLKETDLPKLEQL
                     VVTKSLWDTVDILDRVVGSLVYEHPELEEII"
     gene            complement(149..391)
                     /locus_tag="VQM60_001523"
     CDS             complement(149..391)
                     /locus_tag="VQM60_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET1081381.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIFQKRKKQRLSIGILQTLAGKRILENTNMWLLTI"
     gene            complement(392..1024)
                     /locus_tag="VQM60_001524"
     CDS             complement(392..1024)
                     /locus_tag="VQM60_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1081382.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(1038..2033)
                     /locus_tag="VQM60_001525"
     CDS             complement(1038..2033)
                     /locus_tag="VQM60_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET1081383.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTETLQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(2030..2728)
                     /locus_tag="VQM60_001526"
     CDS             complement(2030..2728)
                     /locus_tag="VQM60_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET1081384.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(2805..3815)
                     /locus_tag="VQM60_001527"
     CDS             complement(2805..3815)
                     /locus_tag="VQM60_001527"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET1081385.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(3799..4806)
                     /locus_tag="VQM60_001528"
     CDS             complement(3799..4806)
                     /locus_tag="VQM60_001528"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET1081386.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(4793..5746)
                     /gene="mvaD"
                     /locus_tag="VQM60_001529"
     CDS             complement(4793..5746)
                     /gene="mvaD"
                     /locus_tag="VQM60_001529"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET1081387.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(5728..6606)
                     /gene="mvk"
                     /locus_tag="VQM60_001530"
     CDS             complement(5728..6606)
                     /gene="mvk"
                     /locus_tag="VQM60_001530"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET1081388.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(6728..7057)
                     /locus_tag="VQM60_001531"
     CDS             complement(6728..7057)
                     /locus_tag="VQM60_001531"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET1081389.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWAQ"
BASE COUNT         2057 a         1737 c         1350 g         2050 t
ORIGIN      
        1 aagattattt cttctagttc agggtgttca tacaccaaac tccctactac tcgatctagg
       61 atatctaccg tgtcccacaa ggattttgtc acgactaact gctctagctt aggcaaatcg
      121 gtttccttta gataagactg cattgctttc aaatagttag cagccacata ttggtatttt
      181 ctaggatcct tttcccagca agtgtctgca aaatcccaat cgataatctt tgtttttttc
      241 gcttctggaa aatattttta tagagtttat ttctttcagg caccgcaata cctagaaaag
      301 aaaattgatg gcgcatatag gcttccatgg accttgcttt tttagagtct tttgctgctt
      361 ctagctcctc aagtaaatct gctaaactca tctaaaactc ctcttgcccc accaaatggt
      421 gctgaaaggc atagacagcc gcctgggtac gatcgctgac ttcaagtttg gcaagaatat
      481 tggacacgtg ggtcttgacc gtcttgagag agataaagag gtcatctgcg atgcgctgat
      541 tttcgtagcc cttggcgatg agttggagaa catctcgctc acgcgcagtc aattcttcat
      601 gaagttccat atgattgcgg tggtattcaa ccttcttgct aacctcttgc tcaatggcca
      661 gctcgccagc agctacctta ctgacggcat gaagcaattc atctgcacta gaagtcttga
      721 gcatatagcc tttggcacca gcatctaaga ctggcatgat tttttcattg tccaaataag
      781 aggtcacaat caaaatcttg gcttcaggcc attctttaag gattgctaag gtcgcgtcaa
      841 ttccattcat ctcaggcatg acaatatcca tgacaatgac atctggacgc agttccaagg
      901 ccaagtcaat cccttgagac ccgttggacg cctcacccac aacttctaca tcgtcttgga
      961 ggtcaaagta gcttttcaaa cccaaacgga ccatttcatg gtcatctact agtaaaattt
     1021 tcatctttac tcctttatca ttccttatct aacaggggaa tacggatatc aactgccagc
     1081 ccttgcttgg gagctgttaa taactgaacc gtccctgcca tatcttcaac ccgctccttg
     1141 atatttcgca gtccataact caagtcgtct aagctcccta actggaaacc aatcccattg
     1201 tccaccacct tcagttgcaa ttcaacatct gtctgataga ggtagacatc taggcaagat
     1261 gcctgggcat ggcggagggt attgctaatc aactcttgca ggatacggaa gatatgctcc
     1321 tcgattttct taggcaattt cgtcatattc tgcttgagac taaccctaag atcactcttg
     1381 tcctcaagct cttttaagag aatctgaatc ccttctatca agctcttctg ctccagttca
     1441 actggtcgca aatgcaagag caaaacccgc aaatccttct gggcagtttc taaaatagct
     1501 gtgacactct gcaactggat ctgcatcttt tctctatcca atttcaaagc ctgctgactg
     1561 atacccgata aaatcatgtg ggccgcaaac aactcctgac tgactgtatc gtgcaaatcc
     1621 cgagcaattc gcttccgttc cttctcgatg atttcctctt cctgagcaag gctctgattt
     1681 tcagcttttt gaagagtctc tgtcaaaagg ttaagtttac ctgataagga cttgaaactg
     1741 gcatccaaat ctggatctgc aacctgaacc acttcttgcc ctgctaataa acgcttgaga
     1801 ttagcctgca tttttcttag agaaagctct tcgatccctc gccaaaacag ggctaagaga
     1861 caggtcatgg acatgctgaa aaccaacaat aaaaagacaa atttttctgt tttttcgaca
     1921 tcgtgcaaaa agatagacca gtcaaaatca agtatttcca gcaagctgtg ggagaaaaaa
     1981 aagacaaata ggaaggaggt gagagcaata atgacatagg cttgtttttt catcctctaa
     2041 ccacctccac atcaccaatc atagtggtca agaaaatctt gacactcttg ttactcttga
     2101 gatagtcttt tgtttcttga tgatagtgtt cattgcggag ggctcgcttg ggctggttga
     2161 aaaaagtcaa atccccatag agacagttaa cgctgagact gacttccaca tctacaggta
     2221 cgatgatttt ggtcgttcct accatctttc tgaggataat gacattgtca tgattggtta
     2281 agatgaccct ctccagatga atagtgtcct tgcccatgaa gcgaaagaga ttgatatcat
     2341 cgaattggca agtctggtag cttgaaaaat gatgaagatt tccaaaccaa cgatttttct
     2401 ccttcttaac cgtcacgacc tcttcaaaaa ccaaattggt ctgctctttt tcctggttca
     2461 tcatcgggta aagaagaaag aggctataga taaccgcaac aaaaatagct agaatcacaa
     2521 aaggattgag cataacgatg aaaaagaaga gaatggttgc cgctactaaa agaagattat
     2581 ttccctcttt accagtgtag tagcgaatca aaagcaaaaa gaggaatagt atcagcagaa
     2641 aacgcgaaaa atgctctgat accatcaaaa tcagagctcc tgtcagaaga caggcttcga
     2701 taaataaaaa gattttaaat tttctcatag gttcatcctc tcccttctat tttatcacaa
     2761 ttcaaaaaag tcacctcagt ctgaggatgg aaaaaaggcg ctggttacgc ctttttcatc
     2821 tgatcctttg cttcttttaa ttttccataa agaagatagt ctactttttg tagatctgct
     2881 atggtggcac agttaaggga acacataatc aagcgtaggt ctgctttcca gccttggaca
     2941 atgccaatca cttcttcaac tgtgtaggtt tcaaccaatt ccagaacggt tcgtgacaat
     3001 cccacagcct tagcaccaaa aaccaagcac ttaatcatat ccagcggatt ccgaacccct
     3061 ccactaacca agagttcgac cttatctttc cattcttggg cattgagaag ggcctgcatg
     3121 gtagactgac cccattgatt gaggtaatca cgctggccac tacgacggtt ttcgatatag
     3181 gcaaagctgg tgccaccacg acccgatagg tccactgtac gaacaccgaa ttcataggct
     3241 ctttcgattg tcttggcatc cattccaaag cccacttcct tgaggacaat aggaacggga
     3301 atttgcttgc tataatctgc tagatgcgat tgccagcttc taaacttcct ttctccctcg
     3361 ggcatgagta attcctgcat gacattgaca tgcacttgca atagaacagg attcatctct
     3421 tctacagtct gaagtcctaa ctcgacaggc ttgtccaatc caatattggt tccaaggagg
     3481 agattgggat gactagactt gacagaaaaa gaatcatccg ttggattttt gagggctgcg
     3541 ctataagaac ccgttacaaa taaaatacca caggattccg ccacctgagc cagcttttga
     3601 ttgatttctc ttcccttatt acttccacca gtcatggcat tgatataaaa aggaaagtcc
     3661 cactttcgac cagcaaactc tgtcgaaaga tcgatttcat ccagattgta aagaggcaag
     3721 gaagaatgaa tcagctccac ctcatcaaag ctattatagg aacttttctg ctcaagggca
     3781 tagaggatat gctcgtcctt acgatttgtc gtcatgtcct atcctttctt gatataagag
     3841 ctcaatcccc agatcggccc aacgattttt taaggttttg gttgattgcg catcaaaact
     3901 cagggcgatg ccacagtcac caccaccagc accactactc ttggcaacgg cctgcaaatc
     3961 ttgactggct tctttcaact gtctaagcaa aggcgtgtaa atatctgtac tcaagccttc
     4021 taaaagcttg ctggctactt ctacttgctc gataatcttt tctgatttcc cctgttccaa
     4081 ggcttcgacc aaagaaacca ccgtttcttt tgaggaactt aaaaaatttt gattgatatt
     4141 ttgcttgatt tgctggacca tgtgactcga tacagccact tccttggtcc atcccactaa
     4201 gaaatcacat tctaaagttg gtttcacttg tgaaattgaa aagccccaat cacgctccag
     4261 aactgtcgcc aagttttctt cttctaacca agcagccacc ttctggcgat caaatgactg
     4321 gtagagaacc aaatcctctg ccacaataca ggcaaggtcg cccatggaac cattgtctcc
     4381 tcgcttgagc aagacagcgc tagtcagctt gaacaagagc tcctgatcaa cagaaacatc
     4441 atacagagcc agtaaagcct tgacaaccaa gacaacgacg ctgccactag aacctagacc
     4501 aaactttttc ccttctcgtt ccattttgcc acagatttct agagaaaaag gtcttaaatt
     4561 ctgaccacga acagcgagga agtctcccat caaagcaatc gtttcttgaa tcaagctgta
     4621 gtcaggatta ggccttaagt ccactgcgaa atcaaacata tctgaataga tacggtagct
     4681 gtcagaaaaa gcaatctcag ccctcatata gatgggaata tcctttatca aagctaactg
     4741 ccctggctct aaaatagcat attcacctgc ccaatagagt tttccgcaag ttttaacagc
     4801 aatcatcttg actcaaatcc tttgtttttg acacaatcaa gcgataacga tgaccgaaaa
     4861 tttctgataa atgctccaag tctttctcct gacagaagac cttaacattg ggaccagcat
     4921 ccatggtaaa gtagcaggcc tctcctttct cacgaagctg gcgaacaaag tccatagcct
     4981 cataagaggc atccgtcaga taagaaaagg ctggactagc agtctttgtc gtagcatgca
     5041 tagccagggc atttttctcc gttaattctc caatcttggc aaaatcattt tccttgagat
     5101 aaatcagcat atcctgatag tccttctcag actgacgaac ccagtcgtca aaagtcgtcg
     5161 aggtttccac acaaagtttc atcccgtcac ggctagagat tggttttttc ttgtcctcta
     5221 gcaccaacat aatcatagct agtttcaagt ctgtctctac agggtaaatt tctccactat
     5281 ccttatccca ggctcctagt ggtccataaa aactccgaga agaagagcct gaggcaaatt
     5341 tggcttcctg tgccaactga cttctatcca atccaagctt gaaataagca ttacaagcct
     5401 tgaccagggc ggacaaacca ctagaacttg aggacaggcc cgctgcagta ggcatattgt
     5461 tttgagtatc gatacggaca aagccctcac cagctggacg ataacggtca ataatcttac
     5521 tcatcttggc atgctcgacc tcattttgta gctgaccatt gatgtaaaat tcgtcagctg
     5581 ttacattggc tggtaaaggc gacaaggtcg tctctgtata catattttcc aaagttagag
     5641 aaatactgct agtagcaggc accatctctt tttctttttt ctttccccaa tatttgataa
     5701 tagcaatatt tgcgtaggaa cgtactgtta caggctctct atccatgtct gaacagctcc
     5761 tttctcttct aatctttctg ctagttcttg tgcgtgtgtc aaattggtta ccaaggctat
     5821 gatacaacct cctagcccac caccgctcat cttggcaccc agagcaccat ggctaagagt
     5881 cgtttcaacc aaaaagtctg cctcagggct actgactcca atttctttta aatgtaaatg
     5941 cgcttgactg aggatttgtc ccagtccttc agcatctttt tgtgaaatcg caacttctgc
     6001 ttgctgggtt aattctccca aggcatgcaa aaacggtagg gcatccttgc ccttattttg
     6061 aaccacttgg atggcttcac gagtatgacc ataaacaccc gtatcggcaa tcaccaaata
     6121 ggcggataaa tccatctcaa gttctgtaaa tcctacgttc ttgataaagc gaataggttg
     6181 gtcactgaga caggtcttag catccaaacc actaggattc atatgggcaa tcatttcagc
     6241 tcgattgacc aagatttcta gtacatcatg aggcagatca gcctgatagt agtcaaatac
     6301 cgcacgaatg gccgctatgc tgatagccgc tgacgaaccc atcccccgtt tctcagggat
     6361 agccgagtca atctcacaac gaatgcaggc ttctgtgata tccaaatact ccagtgaggc
     6421 ataaaccgcc atggacaagg tatcctcctc ataaaggcgc caaggactct ctgcaggaac
     6481 taccttacag gtcacctcca cctccaaaag aggcagggaa atggcaggat aaccgtaaac
     6541 gaccgcatgt tcccctatta aaattatctt actatgtgcc tgaccgacac caactttttt
     6601 tgtcattttt tccttttact agacgaaaaa acgtcttatt tttcatacaa gtattaattc
     6661 tttcctatct attttattat attttcacaa aaaaagcgat tgtttccatt cacaatcgct
     6721 tctttcatta ttgagcccat tcgccattat agttgacaga atagccatct acggtcgtat
     6781 tcactgccaa ggcacctgag ctataagcgt agtaccatct gccattgacc tggaaccaac
     6841 ctgtcgtcat agaacgacga aagaaactcc ataccattaa gtaaagagga aagtcgtgag
     6901 ggagcatgcg ccattgacaa cctgttttag tgacgtacaa agtctcatta acaagtactc
     6961 gtttcggcca tttataggtg cggtgtttgg agaaataggg ttcaatcttc gcccattctt
     7021 gatcgtttaa atcagtatca tatgctttac gtatcataac tctagtttaa catttttttg
     7081 tgaatacagg ttctaaataa tcgaccacga aaatttctta agtggaaaac gccttatgaa
     7141 gtatgctacg ggaaacttat gcacttaatt tgacaattca agatgtaaaa atat
//
LOCUS       DAVAAL010000090         7135 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVAAL010000090 DAVAAL010000000
VERSION     DAVAAL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.091"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            358..519
                     /locus_tag="VQM60_001532"
     CDS             358..519
                     /locus_tag="VQM60_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081390.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            611..2290
                     /locus_tag="VQM60_001533"
                     /pseudo
     CDS             611..2290
                     /locus_tag="VQM60_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2558..3124
                     /locus_tag="VQM60_001534"
     CDS             2558..3124
                     /locus_tag="VQM60_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081391.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSRFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            3446..3634
                     /locus_tag="VQM60_001535"
     CDS             3446..3634
                     /locus_tag="VQM60_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET1081392.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3696..4454
                     /locus_tag="VQM60_001536"
     CDS             3696..4454
                     /locus_tag="VQM60_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET1081393.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     ISEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKSYFEKNEIFKIQQDVIF"
     gene            4512..5366
                     /locus_tag="VQM60_001537"
     CDS             4512..5366
                     /locus_tag="VQM60_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET1081394.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMCISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            5478..6362
                     /locus_tag="VQM60_001538"
     CDS             5478..6362
                     /locus_tag="VQM60_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET1081395.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVIANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            6525..>7135
                     /locus_tag="VQM60_001539"
     CDS             6525..>7135
                     /locus_tag="VQM60_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HET1081396.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFES"
BASE COUNT         2517 a          981 c         1520 g         2117 t
ORIGIN      
        1 attaataact ctcgataaat tttgaacctt aacaattgaa tagaccaaga caaggcgtta
       61 attatttttg gagcgtaata acctatccgt caagatcttt cggctgaata attcggcaaa
      121 aaaagttctg ctaagcaaaa agaaagtgag cgataagtaa gagtttgaat atagggaggg
      181 ataccctatc cgctatgaag aaaatgagga taatgatact tctaaggttg aagccggctc
      241 atccggaata gaggcggagg tgagattcct atgttgctaa tccaaagcac ttgtcaatgt
      301 caaaaaactt aattaaacaa ataattatta gatataaaac tttagaggag gtatatattg
      361 gacaatgact ggaatggatt ggctgattta atagcaaatc tgattgcaaa atatgctggt
      421 gctttagatt tagataatct acccgatcca acgccagcaa aaaatcaaga gatgaaaaat
      481 agttttgaca tggcaaaaac acaaattgag acggactaat aaaagtgtta taatatactt
      541 gaaatgatag tccaaactta aattaaaaaa attatatatg atatagaaaa attatattga
      601 ggttaatact atgtcaaaag aaaaaataaa agtatacctc tatacacgag tatctacgtc
      661 aatacagata gaggggtatt cgttagaggc acaaaaatca cgaatgaaag cttttgctat
      721 ttacaacgat tatgaaattg ttggagagta tgaagatgcc gggaagtctg gcaagtctat
      781 tgaaggtaga atacagttca atcgaatgat ggaagatata aaatctggaa aagatggagt
      841 atcttttgtt cttgtgttta agttatcaag atttgcaaga aatgccgctg atgttctttc
      901 tacacttcag ataatgcagg attatggagt taatcttatc tgcgttgaag atggcattga
      961 ttcatctaaa gatgctggga aactaatgat ttctgtttta taagctgtgg ctgaaataga
     1021 aagagaaaac atccgtattc aaacaatgga aggtcgcatt caaaaagcaa gggaaggaaa
     1081 atggaatggt ggctttgctc cgtatggata taaacttgaa gacggcaagc tgtttataaa
     1141 tgaggaagag gcagttgcaa taagaacgat tttcgaccag tatgtaaata ctacgatagg
     1201 agctaatggg atatctaaat acttagagaa tcatggaatt agaaaaatcc caagacagaa
     1261 tggtaagaat cctttgtttg atgcaggtct tataagaaag atattaaaga atcctgtata
     1321 taatgggaaa atagcatttg gaagaagaac tttagaaaaa gttcatggta caagaaatga
     1381 atataagcag gttgaacaag atgaatatct aatatctgaa gggatacatg aagctatagt
     1441 ttccgatgaa gtttggcaag ctgctcaggt taagctaaaa tctcaagcaa agaaatatga
     1501 gcatgtgaat aaaggaaaag atacacgcac acacttgctt tctggaattg taaaatgccc
     1561 gatatgtgga gtgggaatgt ttgggaacaa gtgtatcaag aaaaagaaag atggcacaaa
     1621 gtataaagat ttttattact atggttgtaa acataggcag atgataagag gtcataagtg
     1681 tacattcagt aagcaaatta gagaagaatt gttagatgat gctgttgcag aggtgattgt
     1741 caagatagta agtaatccga aatttgcttc tatgatgcaa gaaaaaatca acatgaaggt
     1801 ggatacctct gaaatagaaa aagaaataga taattaccaa aaggaattga gaaagagtca
     1861 ttctacaaag tttaagctaa ttgaggaaat agataattta gatgttgaag ataagcatta
     1921 taagagaagg aaacaggatt tagatgatag actatatcgt atgtatgaca aaatagatga
     1981 attagaatca tcactaattg atgcgaaagc aaagaaacag actattgaag ctgaaaagct
     2041 tacaggagat aacatatata aggttctgat ctattttgat aagctctata aagtcatgaa
     2101 tgatgtagag cgtaggcagt taatttcagc tttgatttct gaaattcaag tttatgaaga
     2161 aaaacaatct aacggacaat ggctaaaatc cattactttt aagctaccaa ttatagaaga
     2221 aaatctcaac ataggtttgg acaatgatga acaagttgaa tgtgtaagtt tattagagaa
     2281 acgtagttaa tcctcaaaag gttccccgaa ccttttgagt cccactgacg catcacgctg
     2341 aggcggtatc actgcttgta cgagttgaag catcagcaaa gtaaaagtag atgttcagcc
     2401 aatgcgacga ggtgagaccg aaggtcgata aggtgtgttc tgggtatagg actcgtaaag
     2461 taagtaggaa atatgatgag atgatacaga gagcctgaat tggtcaggtg acgtgcttat
     2521 ttacattagc ctgataatga aaatggagag attatcgatg gttatattgg aattgtacca
     2581 aaatgattat tctaaagatt tagtagcatt tgattccata gaagatggga aagcctttgt
     2641 agctcaaatc ccgggataca ctctggaaac agaagatggt tttgaagtgg agtattttaa
     2701 tcctaaaaac ataccggatt atatggaaat tatttttaac ggaaatattg ttcctttatc
     2761 tagatttatg ttcgatcctg gggaaaatgt aaacattatt tggaaagaga tttcaaattt
     2821 atccctcaaa aatgatagag tgatcgaagg atattcaaaa attgatgcat atgtcgttaa
     2881 caaccatgaa gttaaagcct atgtcgaaac aagagaaacg aactatcgta aagcaaaaga
     2941 cttcttagaa agccgcggat atgaaatcga cagaagcttt ttcggaagtg aagacggtga
     3001 agcgattctt tacagaaaaa aagggatcga agtttggcat ttcttgtgtc acttagaccc
     3061 aatgtttgta gagattgaag atgtagaagg atatgttaaa gaagaagttg gagagattca
     3121 atagtcaacc aaagttcctt gtgagtatat acctttgcta gtaaaagagt caaaacgtta
     3181 taacgaaggg ggggaagaaa acccttgagt gtaagtcttt ttcttgttta gagggaaagt
     3241 attagagtag ccgctcataa tttatactag atttgataac tgatttgaga cagattttat
     3301 ttctataaat attgtgaaaa ttttttaata ccaaagattt aaagttactg tgttaaaaat
     3361 cctctccact tttaaactaa tgtattataa aagccagttg acacaagtgt tacacctgtg
     3421 atagaattaa cttgaggtga tgaaaatggc aaccgataaa aatagaataa tgattagctt
     3481 ggatgataaa aatctagaga aacttgaaaa tttagtagaa gatgctagag atagaagggg
     3541 aatgcgacta acaaagtccc aggttattga attactccta aataccgttg attatttcga
     3601 tgatattatg ggggctattt actcaaaaaa ataagagcaa aattgctatt attttttaca
     3661 aattggtgta acactagtga tataggagtg atgaaatggt tgtttatatt agacaaagta
     3721 aattaccaag tgaggtttca attaataaat acaatgctca agtaggtgcc tacttacaag
     3781 gagaagaagt tattttatat caatcttttt ctgaaataaa agagttaata agtgaagata
     3841 tagttgtaga ttatataatg gagactagag cattactaaa aatgatgggc ttaaacgttc
     3901 cggtttatga ctatcctatt gagcttaaag agttttatgg tcgaaagatt tatgctggta
     3961 ttttaggaga gattgtgaat atacctgata attggggaaa atttattaag cctaaagctg
     4021 gttcaaaagt ctttactgga agagttgtta atggaaccca tgatttaata ggtattggtc
     4081 tacctttcga ctatcctata tggattagtg aggttgtaga attcatagct gaatggcgtt
     4141 gttttgtgtt agatggtcgc gtattagatg ttcgacccta tacaggtgat tatcatgcac
     4201 aatttgatgc aagtgtaatt gatgaagcga tatcatgttg gaaagatgcg ccaatagctt
     4261 atggactaga tatcggtgtt actcgcgatg gcagaacact tgttgttgaa gtaaatgatg
     4321 gttatgcatt gggaaattat ggcctatctc ctttaaaatc aatcaatttt catagagcta
     4381 ggtggaaaga aatggtaaaa tcctattttg aaaaaaatga aatttttaaa atccaacagg
     4441 atgttatttt ctaaaataaa tatggtagat aaccaatata aaaagcatca aaatgaacaa
     4501 ggaggaatct gatgcttgga gcaattattg gagatattgt cggttctgtt tacgaatgga
     4561 acaatatcaa aacaaaggac tttcctttat ttcggaagga ctgctttttt acagatgaca
     4621 cggttatgac ctgtgctgtt gcagaagcaa ttatgaatgg tggacaaaaa gacgatttca
     4681 ttgacgcgat gaagaaatat ggcagaatgt atccgaatgc tgattacggt gctcggttta
     4741 atgcatggct aaacagcgat aaccgtgagc cttataatag ctttgggaat ggatcagcta
     4801 tgtgtatttc tccatgtgct tggatcatgg actgtggttt ttatgcgaaa actggtatgt
     4861 ggccatcatc tagaggactt acgagtcttt ctgcagaggt aactcataat catccagaag
     4921 gtgtcaaagg cgctatggct acagctgatg ctatctttct atgtcgtttt tactttggtg
     4981 gttattgtag agagtacgag caatcaatta acgacaatcc tacagagtgt aaaagacgaa
     5041 ttaaggatta tatagaaaaa gaatacgatt acaatctatc tcaaacttta gatgaaatcc
     5101 gtcctaacta tcgttttaac gaaacatgtc aggaaactgt ccctcaagcc attatcgcct
     5161 ttcttgaaag tagagacttc gaagatgcga taagaaatgc catctcactt ggtggcgaca
     5221 gtgatactct cgcagcaatc acttgtaaca tagcagaggc agcttacggt attcctgatt
     5281 ggatcaagga taaggcctat tcttacttgg atgaaccctt aaaggatgta gttaggcgat
     5341 gggaaaatag aataaaagcg tattaatctc cagtacgtca gatttctctt ttctgtattt
     5401 tcattgtata ataggattac atttacttgc atacgacgaa atgtaaacat ttctgtttaa
     5461 aattaaggat aaaatcaatg aaaaaagcaa tggtaattat caaccctact tctggtggcg
     5521 agaaggcttt ggattacaaa gaaaagctgg agaataaagc aaaagaatac tttgaatatg
     5581 ttgaaaccaa aattaccgaa aaagcgctgg atgcaacaca ttttgctgaa gaagcttctc
     5641 gtgagcagta tgatgcagtg gttgtgtttg gtggagatgg aactgtcaat gaagtcattt
     5701 caggtattga tgagagagac tacattccta agttagggat tatcccaggc ggtacgggta
     5761 acctcattac aaaacttttg gaaatcaatc aagacatcga tggcgcaatt gacgaactgg
     5821 attttgattt aaccaataag attgatatcg gtaaagcaaa tgacaactat tttggttata
     5881 tctttagtat cggttctctg cctgaggcga ttcacaatgt tgaaatagag gacaaaacaa
     5941 aattcggtat tttaacctat gctgtaaata ccatgaagtc tgtcatgaca gatcaggtct
     6001 ttaacattaa ggttgagaca gaaaatggaa attatgttgg tgaagctagc catgttttgg
     6061 tccttttgac aaattacttc gctgataaga aaatctttga agaaaacaag gacggctatg
     6121 ccaacatttt gattctgaaa gatgcctcta tattctccaa attatccgtc attcctgatt
     6181 tattaaaagg ggatgttatc gcaaatgata atatcgagta tatcaaagcg cgtaatatta
     6241 aaatctcttc agatagtgaa ttggagtcag atgttgacgg agataaatca gataacctac
     6301 ctgtagaaat caaagtccta gctcagcgag tagaagtatt ttcaaaaccg aaagaggatt
     6361 agtatataga gaaagccttt tttaaggctt tttgtatact ttaaaagata gttcctttaa
     6421 caacggacat tccttgcaaa tagtttacaa aaatagtata ctggattcat tgagtttgaa
     6481 aacgtttgcg taaaatttga atgaatactt taggagacaa attgatggaa ttgagtgcta
     6541 tttaccatag gcctgagtcg gagtatgcct atctttataa ggataagaaa ctccatattc
     6601 gaattcgaac taagaaaggg gacattgaaa gcatcaactt gcactatggg gaccctttta
     6661 tctttatgga ggagttttat caggatacaa aagaaatggt caagataact tctggtacct
     6721 tatttgacca ttggcaggtt gaagtgtcag ttgactttgc acgtatccag tatctctttg
     6781 agctcagaga tacagaaggt caaaatattt tgtatggcga taaagggtgt gtggaaaatt
     6841 ctctagaaaa tcttcatgca attgggaatg gatttaagtt gccttatctt catgagattg
     6901 atgcctgcaa ggttcctgac tgggtttcaa atacggtatg gtatcagata tttcctgaaa
     6961 gatttgccaa tggcaatgct ctattaaacc cagaagggac tttagactgg gattcatctg
     7021 tcacacctaa gagcgatgat ttctttggtg gtgatttaca ggggattatt gatcatatgg
     7081 attacttgca agacttgggt attactggac tatatctttg tcccatcttt gaatc
//
LOCUS       DAVAAL010000091         7099 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVAAL010000091 DAVAAL010000000
VERSION     DAVAAL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.092"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            7..570
                     /locus_tag="VQM60_001540"
     CDS             7..570
                     /locus_tag="VQM60_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--hexose-1-phosphate uridylyltransferase"
                     /protein_id="HET1081397.1"
                     /translation="MRLQSENKNRLLDLATKILNKWREYSDLEVDIIAMSEDIPHHTV
                     TPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKENIGLIEVMGLAILPP
                     RLKTELEEVGKYLLGEDNAIAAYHLEWADQLKEKYSGINKEEVNSVVQHEAGQVFARV
                     LEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            583..1593
                     /gene="galE"
                     /locus_tag="VQM60_001541"
     CDS             583..1593
                     /gene="galE"
                     /locus_tag="VQM60_001541"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET1081398.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVVIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            1760..2419
                     /locus_tag="VQM60_001542"
     CDS             1760..2419
                     /locus_tag="VQM60_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET1081399.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRVTEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVRFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            2444..3511
                     /locus_tag="VQM60_001543"
     CDS             2444..3511
                     /locus_tag="VQM60_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET1081400.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIPGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSISGLQGGTGRAILASILVRYLDDKGELGVSEKGWEVAKEYLKNAYTL
                     QKGESSIVKMLDKEDPIQYGMMWGSGALVGQKEQNVVFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKNFGSIPANKDALTELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            3614..4624
                     /locus_tag="VQM60_001544"
     CDS             3614..4624
                     /locus_tag="VQM60_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081401.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSENQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            4636..6327
                     /locus_tag="VQM60_001545"
     CDS             4636..6327
                     /locus_tag="VQM60_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET1081402.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKARFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            6330..7040
                     /locus_tag="VQM60_001546"
     CDS             6330..7040
                     /locus_tag="VQM60_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET1081403.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2357 a         1001 c         1404 g         2337 t
ORIGIN      
        1 tcagtaatta ggttacaaag tgaaaataaa aatcgcttgt tagatttagc tactaaaatt
       61 ctaaataagt ggagagaata ttcagattta gaagtagaca tcattgctat gtctgaagat
      121 attccacacc atacggtgac tcctattgct cgtaaagtag atgggagata tgaattggat
      181 attgttctga gagataatca tactactgaa caatacccag atggtgtttt ccatcctcat
      241 caagatgtac aacatattaa aaaggaaaat attgggttaa tcgaagtgat gggacttgcg
      301 atattaccac ctcgtttgaa aacagagtta gaagaagttg gaaaatatct tttaggagaa
      361 gataatgcaa ttgcggctta tcatttagaa tgggccgacc aattgaaaga aaaatattct
      421 ggtattaaca aagaagaggt taatagcgta gttcaacacg aagcaggaca agtgtttgcg
      481 agagttcttg aagatgcagg tgtttataag aatactccgt ctggacatga agcgtttatg
      541 agatttgtta aatcagttgg aattaattag gaggaaaccc aaatggcaat attggtaaca
      601 ggcggtgctg gttatattgg tagccatacc gtagtagaat tactaaattt aggaaaggaa
      661 gtcgtcattg ttgacaacct ttctaactct agcatcttgg tgttagaccg tattgaagca
      721 attacaggaa tacgtcccgt gttttacgaa ttagatgttt gtgataaaca agcattgaga
      781 aaggtatttg aacaagaatc gattgatgct gcaattcatt ttgcaggtta taaagctgtc
      841 ggagaatccg tgcaaaagcc tgtgatgtac tacaaaaata atattatgag tacattggca
      901 cttgttgaag tgatgtcaga atttaatgtt aaaaagattg tcttttcttc aagtgcgact
      961 gtatatggaa ttaacaatca gtcacctcta attgagacga tgcaaacaag tgcgacaaat
     1021 ccttatgggt atacgaaagt gatgcttgag caaattttaa aagatgttca tgtggcagat
     1081 tcagaatgga gtattgcgtt gcttcgttat ttcaatccaa ttggtgctca tgagtctggt
     1141 ttgattgggg aagatccctc aggaattcct aacaacttga tgccctatat tgcgcaagta
     1201 gcggtaggta agctgtcaga gctaagtgtg ttcggaaatg attatgatac gcttgatggt
     1261 actggtgtgc gtgattatat ccatgtagta gatttagcaa taggacatat aaaagcttta
     1321 gaaaaagtat ctgaaaaaac agatgtttat atctataacc taggctcggg agaaggcact
     1381 agtgtattac aacttgtaaa tacatttgaa agtgttaata agatccctat tccttataaa
     1441 atagttccaa gacgttcagg agacgttgca acttgttatg caaatgcaga caaagcatat
     1501 aaggaattaa attggaggac aacaaaatcg attgaagaca tgtgtagaga tacatggaat
     1561 tggcaatcaa aaaatcccaa tggctataat tgatgatact gtctatatga gtgttgtatt
     1621 ttgggaaaat tacttaacct tttaatgaaa gataattttt taaaaaaatg tttgaaagcg
     1681 cttgacaaag taaacggttt cgttatataa tataagcata gatgacaccg gtgtcaaata
     1741 taaaaaagag ggtctttata tgaaaaacct aacaaaaatt aagttcaaag aaaatggaga
     1801 atttaatcat tttccaggaa atactgttgt agcaaatctt tatactaaac aagatttgat
     1861 ggaagttgtt gatattattc aatcacgtta tagagaatta ccatttatcg ataagtttac
     1921 tttaactcca aggaattcta ttcacatgac tgtaattgaa ttgttgtgcc atgaaaatcg
     1981 tgaaacggag ttttggagca gtaatcttcc tcttgataca cctttacagg aaatacatga
     2041 ttactttgct aaacaacttg aaatttttcc attgttggat gaagaaattc atatgcgtgt
     2101 gactgaaatg ggaaaacaaa acatactagt tgaacctgca gatgaagctt ctgcaaagag
     2161 attagaagaa attcgtactt atgtttcaga aaaagcaggt gttcgtttcc ctaatcatga
     2221 tagataccaa ttccatattt caattggata tcttcggatt cctctaaccg aagaggagga
     2281 agaagagttt actaaagtca gagcagaatt aactgaaatt ttattagaga agattccaac
     2341 tattactgta aaccgtattg actatactgt atttgaagat atgagacaat ttgttccata
     2401 tcacgaaaaa tttaaataaa ataataaaaa aggagaactg atcatgaaaa aaatgaaagt
     2461 ttggtctact gtacttgcaa cgggagttgc tcttactaca cttgctgctt gctctggagg
     2521 ttcaaattct acgactgctt cttcatctga agaaaaagct gataaaagtc aagaattagt
     2581 aatctattcg aactcagtct caaatggtcg tggtgattgg ttaactgcta aagcaaaaga
     2641 agctggtttt aatatcaaaa tggttgatat ccctggcgct caattagcag accgtgttat
     2701 tgctgagaag aataatgcag ttgcagatat ggtatttgga attggtgctg ttgattcaaa
     2761 taaaattaga gatcaaaaat tactagtaca gtacaagcct aaatggttag ataaaattga
     2821 tcaatcttta tcagataaag ataattatta taatcctgtg attgttcaac cattagtttt
     2881 aattggggcg cctgatgtaa aagaaatgcc taaagattgg acagaattag gtagtaagta
     2941 taaaggtaaa tattcaattt ctggtcttca aggtggtaca ggacgtgcaa ttctagcaag
     3001 tatcttagtt cgataccttg atgataaagg tgaattaggt gtttccgaaa aaggttggga
     3061 agtagcaaaa gaatatttga aaaatgcata cactcttcaa aagggagaaa gttcaattgt
     3121 taagatgtta gacaaagaag atccaataca atatggaatg atgtggggtt ctggtgcatt
     3181 agttggacaa aaagaacaaa atgttgtttt caaagttatg actcctgaga ttggtgtacc
     3241 atttgtaact gaacaaacta tggttttaag cactagtaaa aaacaagcgt tagctaaaga
     3301 atttattgat tggtttggtc aatcagaaat tcaagtagaa tatagtaaga actttggatc
     3361 tattcctgca aataaagatg ccctaacaga gttacctgaa gatacgaaaa aatttgttga
     3421 tcaagtgaaa ccacaaaata ttgactggga agctgttgga aaacatttgg atgaatgggt
     3481 agaaaaagct gaattagaat acgtacaata agaaataaga gttaattttt atgtccaagt
     3541 ctctctatta tttctaaatt tatagaagaa ataaagagag gcttttggtt tagtaataga
     3601 gggagttagt gagatgatta aatttgataa tattcaaatt aaatatggtg attttgttgc
     3661 aattgataat ctgaatttag atatacatga aggggaattt tttacatttc ttgggccttc
     3721 aggatgtggt aaatcaacta ctttgagagc attggtaggt tttctagatc catcatcagg
     3781 aagtattgaa gttaatggaa cagatgtcac tcatttggaa cctgaaaagc gtggaattgg
     3841 tattgtattt caatcttatg ctctatttcc aactatgact gtttttgata atattgcatt
     3901 tggtttaaaa gttaagaagg tagctccaga tgttattaaa gctaaagtat cagcagtagc
     3961 agcaaaaatt aagatctctg atcaacagtt acagcgtaat gtatcagaat tatctggggg
     4021 tcaacaacaa agggtagcgt tggctcgtgc tctggttctt gaacctaaaa ttctttgtct
     4081 agatgaacca ttgtcaaacc ttgacgcaaa attacgtgta gatttgagaa aagagttgaa
     4141 aagacttcaa aaagagttag gtattactac tttatatgtt actcatgatc aagaggaagc
     4201 cttgactcta tctgatagaa ttgcagtctt taacaatgga tacatcgaac aggtcggtac
     4261 accagtagag atttatcata attctcaaac tgaatttgta tgtgatttta ttggagatat
     4321 taatgttttg accgatgaaa cagtccacga agtattactg aaaaatacaa gcgttttctt
     4381 agaagataaa aaaggataca ttcgattaga gaaagttcga ttcaatcgtg aaactgaaca
     4441 agattttatt ctaaaaggga cgattattga tgttgagttt tctggagtta caattcacta
     4501 tacaataaaa gtttctgaaa atcagattct taatgtaaca agtattgata gtcaggctgc
     4561 tattagatct gtcggagaaa gtgtggaatt atttatcaca ccatcagacg ttctgcaatt
     4621 ttaaggaggt gcagtatgcg tcataaatta aatttaaaag attggcttat tcgtttaggg
     4681 ttaatctggt tcttagtaac atttattatt tatccaaact ttgatctagt agtgaatgta
     4741 tttgtaaaag gaggagaatt ttcccttgat gctgtacatc gtgttctaaa atctcagagg
     4801 gcacttcaga gtattatgaa cagttttaag ttagcatttt cactcattat tacagttaat
     4861 gtcgtaggta ttctttgtgt tctatttaca gagtactttg atattaaagg tgctaaaatt
     4921 ttaaaattag gttatatgac ctctttaatt tatggaggag tggttttagc gactggatat
     4981 aaatttgtct atggtcctta tggattgata acaaaatttt tacaaaatgt tatcccttct
     5041 ttagacccta actggtttat tgggtatggt gcagtcttat tcattatgac attttcagga
     5101 actgctaatc atacattgtt tttaacaaat acaattcgaa gcgttgacta tcacactatt
     5161 gaggctgctc gaaatatggg agcaaaacca tttactgttt tccgaaaagt agtgttacca
     5221 accttaattc caactctatt tgcacttact attatggttt ttcttagtgg tttatctgca
     5281 gtagcagcac ccatgattgt tggtggtaaa gaatttcaaa ctataaatcc aatgattatt
     5341 acatttgcag ggatggggaa ttctcgtgat ttagctgccc tacttgcaat tattttaggt
     5401 attgcaacta caattttgct tactatcatg aataagatag aaaaaggtgg aaattatatt
     5461 tctatctcta agactaaagc gcctcttaaa aaacaaaaaa ttgcgtctaa gccttggaat
     5521 atcattgctc acattgtagc atatggattg ttcacagttt tcatgcttcc actaattttt
     5581 atagtattat actcatttac agatccagtt gcaattcaaa caggtaactt aacattatca
     5641 aactttactt tagaaaatta tcgcctattc tttagtaata gtgcggcatt ctctccattc
     5701 ttggtcagct ttatttattc tattatcgct gcgacaacag caacaattct cgcagttgta
     5761 tttgctcgag ttgtcagaaa acataaagct cgttttgatt ttttatttga atatggtgct
     5821 ctacttcctt ggttactacc aagtacactt ttagcagtaa gtttattatt tacttttaat
     5881 cagccacaat ttcttgtctt gaatcagatt ttggtaggta gtttggtaat tctacttatt
     5941 gcatatatag ttgtaaaaat cccattttct tatagaatgg tacgtgctat tttatttagt
     6001 gttgatgatg agatggaaga tgcagcaaga agtatgggtg cttcaccttt ttatactatg
     6061 atgaaggtta tcattccatt tattttaccg gttgttctct ctgttattgc tttaaacttt
     6121 aactctttat taactgactt cgacttatct gtattccttt accatcccct agctcaacca
     6181 ttaggtatta cgattcgatc tgcaggtgat gaaacagcaa catctaatgc acaagctctg
     6241 gtatttgttt atacaattgt tctgatgatc atttctggaa cggtattata cttcacacaa
     6301 agaccggggc gtaaagtaag gaaataatca tgacagccac tagtcttggg ttatcaaata
     6361 ttgaaatagt tgtcaggatt gttttatcag tagtcattgg tagtataatt ggtttagaga
     6421 gagggagcaa atcccagcct gcaggcatcc gaacttatag tattgtttgt ctagctgcat
     6481 gtttgattat gatgacgaat gaatacgtat cttataaatt tgggacagga gatcctacac
     6541 gattaggagc tcaagttata tcaggtgtgg gttttctagg cgctggaacg attcttatta
     6601 cagataaaaa gaaaattaca ggtctgacaa ctgcagcagg catttgggct tcggcaggaa
     6661 ttggattagc tattggagta ggtttttatg agggagctct tttagtagcc atttctgttt
     6721 ggggtgtgat atccatgttc caaccactaa aaaaatatct gcaaaatcgt tctaaaatga
     6781 ttgaattgta tatagtggtt aaatctacag aagcctataa tcgtgtattg gtttattgtg
     6841 cagaaaatgg tatcagaatg accgattcaa gaacagcgtt tggagatgtt aattctgaca
     6901 gaattgaata ctttgatgtt ccagataaaa aaatagcgtc atttattact ctggaattat
     6961 caggtagatt tgaacatctt cgactgatgg aagaaattgc taatattgta ggtgttattt
     7021 atgtcgaaga aattagctga aatttagtaa acaaagggac agatgttttt gtccttttgt
     7081 tttttagatg ttttaatca
//
LOCUS       DAVAAL010000092         7069 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVAAL010000092 DAVAAL010000000
VERSION     DAVAAL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.093"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(373..2064)
                     /locus_tag="VQM60_001547"
     CDS             complement(373..2064)
                     /locus_tag="VQM60_001547"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET1081404.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGIGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2200..2646
                     /gene="argR"
                     /locus_tag="VQM60_001548"
     CDS             2200..2646
                     /gene="argR"
                     /locus_tag="VQM60_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET1081405.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDKWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2697..5231
                     /gene="mutS"
                     /locus_tag="VQM60_001549"
     CDS             2697..5231
                     /gene="mutS"
                     /locus_tag="VQM60_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET1081406.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEHISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5378..>7069
                     /locus_tag="VQM60_001550"
     CDS             5378..>7069
                     /locus_tag="VQM60_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081407.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGIMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKVVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         1918 a         1381 c         1670 g         2100 t
ORIGIN      
        1 cttcggatta cgaatgaatt tgattgctgg cattatcaat catgaactag gattctagtt
       61 ttgcaggaag tctattattt ggttaggtga attagtgaag cgtttaggca agtgtctctg
      121 gttacgacgt catggactct aaatcgatta tatttagggg tcatgactag tgaagcagtt
      181 agctagttcg catataagcg gctagcgtct aacaattagg aactttagtt ccaataactt
      241 taagattacg acgttttagg acataaatcg atcatattta tgtcctaaaa ctagtgaagc
      301 gcctagccaa agtccgaata ggatttggcg ttagttactt agattgcttt gcaatcaagt
      361 aactttggcg atttacatct tctctggcgc ttctactcca agcaagcgaa gggcttcttt
      421 gagaacgact gcggttgcgt agctgagggc tagacggctg tcgcgttctg ggctttcatc
      481 caagatacgt gtatgtgcat agtatttgtt aaaggattga gccaggctaa ttgcaaattt
      541 agcaatgata gaaggttcaa agttatctgc cgcacggttg ataatacgtg ggaagtcttg
      601 aatgagttta atgatttccc agctttcagt atcattcaag ctatagttgc cagctgtttc
      661 tggtttgaaa tcggctttgc gtaagataga ttggatacga gcgtaggcat attgaacgta
      721 aggtccagtt tcaccctcga aggataccat agcctctagg tcgaagtcgt atccatttgt
      781 acggtcggtt ttgaggtcat agaatttaat ggctccaatc ccaacagcat gtgctacttg
      841 gtctttgttt tctagttcag gatttttagc ctcgatttgg accttggcac ggctaacagc
      901 ctctgcaaca gtaggctcta gcaagatgac attcccttta cgagtagaga gtttcttccc
      961 ttcttttgta accaaaccaa aaggaacgtg agtaatgtcg tcactccagt cgtagcccat
     1021 ctcttgcaag acagctttga gctgtttaaa gtgggcagat tgttcttgac caacgacata
     1081 gatagattta gcaaattggt attcgttttt acggtagagg gctgcagcca agtcacgtgt
     1141 gatatagaga gttgcaccat cagacttctt gatgagggct ggatgttcaa ttccatattt
     1201 ctcaagattc acaacttggg caccttctga ttcaagaagt agtccttttt cagaaagaat
     1261 gtctacaact gcatccatct tatcattgta gaaggcttct ccgttatagc tgtcaaattc
     1321 aaccttcaat tcattgtaaa ggcggttaaa ttccactaaa ctttcatcgc ggaaccattg
     1381 ccaaagagcg agagcttcct catctccatt ttcaagttta cggaaccatt cgcgcgcttc
     1441 ttcatccaag ctagggtcat tttcagcttc agcgttgatg cggacataga gtttaaggag
     1501 ttcatcgatt ggatgagctt ttacagcttc ttcgtcgccc cattttttgt aggcaacaat
     1561 caacatccca aattgtttac cccagtctcc caaatggttg accttgaccg tttgataacc
     1621 gattttttgg aaaatatgtg acaagctatc tccgataaca gttgaacgca ggtggccaat
     1681 agaaaatggt ttagcgatat tcggactaga catgtcgata acaacatttt cttgtttacc
     1741 aatattttgg tcagcatagt gttctttttc agtggtaaca gcttgcaata cttgagcaga
     1801 aatggcagat ttatcaagga aaaagttaac gtaaggtcct gttgcgacaa ctttttcaaa
     1861 ggcttggctg ttcatttttt cagccagttc agccgcaatc atttgtggtg ctttacgttc
     1921 gactttggca agagaaaaag cagggaaagc gatgtcaccc atttctgagt ttttaggggt
     1981 ttccagtaac tttaaaatag cctcttggtc caggctatca atgatgctag ccaattcgct
     2041 agcaatcaat tcttttgtat tcattaagag ctcctttttg gacttttcta ctattttatc
     2101 acaattttaa agaaagaaga aaaaattttt gaaatctcct gtttttttgg tataatatgg
     2161 ttataaatat agttataaat atgcacgcaa gaggatttta tgagaaaaag agatcgtcat
     2221 cagttaataa aaaaaatgat tactgaggag aaattaagta cacaaaaaga aattcaagat
     2281 cggttggagg cgcacaatgt ttgtgtgacg cagacaacct tgtctcgtga tttgcgcgaa
     2341 atcggcttga ccaaggtcaa gaaaaatgat atggtgtatt atgtactagt aaatgagaca
     2401 gaaaagattg atttggtgga atttttgtct catcatttag aaggtgttgc aagagcagag
     2461 tttaccttgg tgcttcatac caaattggga gaagcctctg ttttggcaaa tattgtagat
     2521 gtaaacaagg ataaatggat tttaggaaca gttgctggtg ccaatacctt attggttatt
     2581 tgtcgagatc agcacgttgc caaactcatg gaagatcgtt tgctagattt gatgaaagat
     2641 aagtaaggtc ttgggagtta ctctcaagac ttatttttga aaaggagaga cagaaaatgg
     2701 cgatagaaaa gctatcaccc ggcatgcaac agtatgtgga tattaaaaag caatatccag
     2761 atgctttttt gctctttcgg atgggtgatt tttatgaatt attttatgag gatgcggtca
     2821 atgctgcgca gattctggaa atttccttaa cgagtcgcaa caagaatgcc gacaatccga
     2881 tccctatggc gggtgttccc tatcattctg cccaacagta tatcgatgtc ttgattgagc
     2941 agggttataa ggtggctatc gcagagcaga tggaagatcc taaacaagca gttggggttg
     3001 ttaaacgaga ggttgttcag gtcattacgc cagggacagt ggtcgatagc agtaagccgg
     3061 acagtcagaa taattttttg gtttccatag accgcgaagg caatcaattt ggcctagctt
     3121 atatggattt ggtgacgggt gacttttatg tgacaggtct tttggatttc acgctggttt
     3181 gtggggaaat ccgtaacctc aaggctcgag aagtggtgtt gggttatgac ttgtctgagg
     3241 aagaagaaca aatcctcagc cgccagatga atctggtact ctcttatgaa aaagaaagct
     3301 ttgaagacct tcatttattg gatttgcgat tggcaacggt ggagcaaacg gcatctagta
     3361 agctgctcca gtatgttcat cggactcaga tgagggaatt gaaccacctc aaacctgtta
     3421 tccgctacga aattaaggat ttcttgcaga tggattatgc gaccaaggct agtctggatt
     3481 tggttgagaa tgctcgctca ggtaagaaac aaggcagtct tttctggctt ttggatgaaa
     3541 ccaaaacggc tatggggatg cgtctcttgc gttcttggat tcatcgcccc ttgattgata
     3601 aggaacgaat cgtccaacgt caagaagtag tgcaggtctt tctcgaccat ttctttgagc
     3661 gtagtgactt gacagacagt ctcaagggtg tttatgacat tgagcgcttg gctagtcgtg
     3721 tttcttttgg caaaaccaat ccaaaggatc tcttgcagtt ggcgactacc ttgtctagtg
     3781 tgccacggat tcgtgcgatt ttagaaggga tggagcaacc tactctagcc tatctcatcg
     3841 cacaactgga tgcaatccct gagttggaga gtttgattag cgcagcgatt gctcctgaag
     3901 ctcctcatgt gattacagat gggggaatta tccggactgg atttgatgag actttagaca
     3961 agtatcgttg cgttctcaga gaagggacta gctggattgc tgagattgag gctaaggagc
     4021 gagaaaactc tggtatcagc acgctcaaga ttgactacaa taaaaaggat ggctactatt
     4081 ttcatgtgac caattcgcaa ctaggaaatg tgccagccca ctttttccgc aaggcgacgc
     4141 tgaaaaactc agaacgcttt ggaaccgaag aattagcccg tatcgaggga gatatgcttg
     4201 aggcgcgtga gaagtcagcc aacctcgaat acgaaatatt tatgcgcatt cgtgaagagg
     4261 tcggcaagta catccagcgt ttacaagctc tagcccaagg aattgcgacg gttgatgtct
     4321 tacagagtct ggcggttgtg gctgaaaccc agcatttgat tcgacctgag tttggtgacg
     4381 attcacaaat tgatatccgg aaagggcgcc atgctgtcgt tgaaaaggtt atgggggctc
     4441 agacctatat tccaaatacg attcagatgg cagaagatac cagtattcaa ctgattacag
     4501 ggccaaacat gagtgggaag tctacctata tgcgtcagtt agccatgacg gcggttatgg
     4561 cccagctggg ttcctatgtt cctgctgaaa gcgcccattt accgattttt gatgcgattt
     4621 ttacccgtat cggagcagca gatgacttgg tttcgggtca gtcgaccttt atggtggaga
     4681 tgatggaggc caataatgcc atttcgcatg cgaccaagaa ctctctcatt ctctttgatg
     4741 aattgggacg tggaactgca acttatgacg ggatggctct tgctcagtcc atcatcgaat
     4801 atatccatga gcacatcgga gctaagaccc tctttgcgac ccactaccat gagttgacta
     4861 gtctggagtc tagtttacaa cacttggtca atgtccacgt ggcaactctg gagcaggatg
     4921 ggcaggttac cttccttcac aagattgaac cgggaccagc tgataaatcc tacggtatcc
     4981 atgttgccaa gattgctggc ttgccagcag accttttagc aagggcggat aagattttga
     5041 ctcagctaga gaatcaagga acagagagtc ctcctcccat gagacaaact agtgctgtca
     5101 ctgaacatat ttcactcttt gatagggcag aagagcatcc tatcctagca gaattagcta
     5161 aactggatgt gtataatatg acacctatgc aggttatgaa tgtcttagta gagttaaaac
     5221 agaaactata aaaccaagac tcactagtta atctagctgt atcaaggaga cttctttgac
     5281 aattctccac ttttttgcta gaataacatc acacaaacag aatgaaaagg agctgacgca
     5341 ttgtcgctcc cttttgtcta ttttttaagg agaaagtatg ctgattcaga aaataaaaac
     5401 ctacaagtgg caggccctgg cttcgctcct gatgacaggc ttgatggttg ctagttcact
     5461 tctgcaaccg cgttatctgc aggaagtctt aggcgccctc cttactggga aatatgaagc
     5521 tatttatagt atcggggctt ggttgattgg tgtggccgta gtcggtctag ttgctggtgg
     5581 actcaatgtt gtcctcgcag cctatattgc ccaaggagtt tcatccgacc ttcgggagga
     5641 tgccttccgt aaaattcaaa ccttttctta tgctgatatt gaacaattta atgcgggaaa
     5701 tctagtcgtt cgaatgacaa atgatatcaa ccagattcag aacgttgtca tgatgacctt
     5761 ccaaattctt ttcagacttc ccctcttgtt catcggttcg tttatcctag cggttcaaac
     5821 cttaccttct ctgtggtggg tgattgttct catggtagtc ttgatttttg gtttgactgc
     5881 tgtcatgatg ggaataatgg ggcctcgttt tgccaagttt caaacccttc ttgagcgcat
     5941 caatgccatt gccaaggaaa atttacgtgg cgttcgtgtg gtcaagtcct ttgtccaaga
     6001 aaaagagcaa tttgctaagt ttacagaggt ctcagacgag cttcttggtc aaaaccttta
     6061 cattggttat gccttttcag tagtggaacc ctttatgatg ttggttggtt acggggcggt
     6121 cttcctctct atttggctgg tcgcgggaat ggttcagtcg gatccgtctg ttgttggttc
     6181 catcgcttct tttgttaatt acctaagcca gattatcttt accattgtta tggttggatt
     6241 tttgggaaat tctgtcagcc gtgccatgat ttccatgcgt cgtattcgag aaattcttga
     6301 cgcagagcca gctatgacct tcaaggatat cccagatgaa gagttggttg gaagtcttag
     6361 ctttgaaaat gtgaccttta cctatccaat ggacaaggaa ccgatgctga aagttgtgag
     6421 ctttactatt gaacctggtc aaatggttgg tgtagttgga gcgactggtg caggaaagtc
     6481 aaccttggct caattgattc cacgtctctt tgatccacag gacggggcca ttaaaatcgg
     6541 tggcaaggat attcgagaag tgagtgaagg aaccctgcgt aaaacagttt ccatcgttct
     6601 ccaacgtgcc attcttttta gtggaacgat tgcagataac ttgagacagg ggaaggggaa
     6661 tgctactcta tttgaaatgg agcgcgcagc caatattgcc caggctagtg aattcattca
     6721 tcgtatggag aaaacctttg aaagtccagt tgaagaacgg ggaaccaatt tctctggtgg
     6781 acaaaaacaa aggatgtcga ttgcgcgtgg gattgtcagc aatccacgta ttctgatttt
     6841 tgatgattcg acctcagcct tggatgccaa atcagagcgc ttggtgcaag aagctttgaa
     6901 taaggacttg aaggggacga caaccattat tattgctcaa aaaattagct cggttgtcca
     6961 tgcagacaag atcttggttc taaatcaagg acgattgatt ggtcaaggta cgcatgcaga
     7021 cttggttgcc aacaatgccg tttaccgtga aatctatgaa acacagaaa
//
LOCUS       DAVAAL010000093         6985 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVAAL010000093 DAVAAL010000000
VERSION     DAVAAL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.094"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..4864
                     /locus_tag="VQM60_001551"
     CDS             <1..4864
                     /locus_tag="VQM60_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HET1081408.1"
                     /translation="RIVEKGTKKTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAV
                     VSDKGEPEVQPTLPEAVVTDKGETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVK
                     PETPVEKTKEQGPEKTEEVPVKPTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNS
                     EKVSPDTSSENTGEVSSNPSDSTTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTV
                     EGTSNQETEKPVQPAEETQTNSGKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATK
                     PENSGNTTSENGQTEPEPSNGNSTEDVSTESNTSNSNGNEEIKQENELDPDKKVEEPE
                     KKLELRNVSDIELYSQTNGTYRQHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITE
                     EKRNGQSVYKITAKAEKLQQELENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQN
                     PSGTYHLAASLNANEVELGPDERSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSG
                     ATVEKLSLKNVAISGKDDIGSLANEAQNNTKIKQVHVDGVLAGERGIGGLLAKAEQSS
                     ITESSFKGRIINTYETTAAYNIGGMVGHLTGDKALLTKSKATVAISSNTNTSDQTVGG
                     LAGLVDRDAQIQDSYAEGDINNVKHFGRVAGVAGNLWDRTSGDVRYAGSLTNVLSDVN
                     VTNGNAITGYHYNEMKVKDTFSSKANRVYNVTLVKDEVVSKESFEERGTMLDASQIAS
                     KKAEINPFILPTVEPLSTSGKKDSDFSKVAYYQAKRNLTYKNIEKLLPFYNKATIVKY
                     GNLVNENSLLYQKELLSAVMMKDNQVITDIVSNKQTANKLLLHYKDDLSEKLDLKYQN
                     DFAKLAEYSLGNTGLLYTPNQFLYDQTSIIKQVLPDLQKVDYHSEAIRKTLGISPNVK
                     QTELYLEDQFAKTKQQLEDSLKKLLSADAGLASANPVTEGYLVDKIKRNKEALLLGLT
                     YLERWYNFSYGQVNVKDLVLYHLDFFGKGNASPLDTLIELGKSGFNNLLAKNNVDTYG
                     ISLASQHGTTDLFSTLEHYRKVFLPNTSNNDWFKSETKAYIVEEKSTIAEVKAKQKQA
                     GTKYSIGVYDRITSNTWKYRNMVLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKA
                     GKALNDFVEENARETAKRQRDHYDYWYRILDEQSREKLYRTILLYDAYKFGDDTTSGK
                     ATAEAKFDSSNPAMKNFFGPVGNKVVHNQHGAYATGDGVYYMSYRMLDKDGAITYTHE
                     MTHDSDQDIYLGGYGRRSGLGPEFFAKGLLQAPDQPSDATITINSILKHSKSDSTEGS
                     RLQVLDPTERFQNAADLQNYVHNMFDLIYMMEYLEGQSIVNKLSVYQKIEALRKIENQ
                     YLTDPADGNDVYATNVVKNLTEDEAKKLTSFDSLIDNNILSAREYKAGTYERNGYFTI
                     KLFAPIFSALSGEKGTPGDLMGRRIAFELLAAKGFKDGMVPYISNQYEEDAKQQGQTI
                     NLYGKERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWP
                     RYGTKTINNVDELQKLMDEAVLQDATGTRWSNYNPETDSAVHKLKRAIFKAYLDQTND
                     FRSSIFENKK"
     gene            4916..5092
                     /locus_tag="VQM60_001552"
     CDS             4916..5092
                     /locus_tag="VQM60_001552"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET1081409.1"
                     /translation="MQAGIPPDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            5312..6163
                     /gene="ylqF"
                     /locus_tag="VQM60_001553"
     CDS             5312..6163
                     /gene="ylqF"
                     /locus_tag="VQM60_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET1081410.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LIKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            6150..6929
                     /locus_tag="VQM60_001554"
     CDS             6150..6929
                     /locus_tag="VQM60_001554"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET1081411.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
BASE COUNT         2442 a         1332 c         1457 g         1754 t
ORIGIN      
        1 aagaatagtc gaaaaaggta ctaaaaaaac tcaagttata aaggaacaac ctgagactgg
       61 tgtagaacat aaggacgtac agtctggagc tattgttgaa cccgcaattc agcctgagtt
      121 gcccgaagct gtagtaagtg ataaaggcga accagaagtt caaccgacat tacccgaagc
      181 agttgtgacc gacaaaggtg agactgaggt tcaaccagag tcgccagata ctgtggtaag
      241 tgataaaggt gaaccagagc aggtagcccc attgccagaa tataagggta atattgagca
      301 agtaaaacct gaaactccgg ttgagaagac caaagaacaa ggtccagaaa aaactgaaga
      361 agttccagta aaaccaacag aagaaacacc agtaaatcca aatgaaggta ctacagaagg
      421 aacctcaatt caagaagcag aaaatccagt tcaacctgca gaagaatcaa caacgaattc
      481 agagaaagta tcaccagata catctagcga aaatactggg gaagtgtcca gtaatcctag
      541 tgattcgaca acctcagttg gagaatcaaa taaaccagaa cataatgact ctaaaaatga
      601 aaattcagaa aaaactgtag aagaagttcc agtaaatcca aatgaaggca cagtagaagg
      661 tacctcaaat caagaaacag aaaaaccagt tcaacctgca gaagaaacac aaacaaactc
      721 tgggaaaata gctaacgaaa atactggaga agtatccaat aaacctagtg attcaaaacc
      781 accagttgaa gaatcaaatc aaccagaaaa aaacggaact gcaacaaaac cagaaaattc
      841 aggtaataca acatcagaga atggacaaac agaaccagaa ccatcaaacg gaaattcaac
      901 tgaggatgtt tcaaccgaat caaacacatc caattcaaat ggaaacgaag aaattaaaca
      961 agaaaatgaa ctagaccctg ataaaaaggt agaagaacca gagaaaaaac tcgaattaag
     1021 aaatgtttct gatattgagt tgtatagtca gacgaatgga acctacagac aacatgtttc
     1081 attggatgga attccagaga atacggatac ttactttgtc aaggtaaaat cttcagcatt
     1141 taaagatgtc tatataccag tagcttcaat aactgaagag aaaagaaatg ggcagtcagt
     1201 ttataaaatc acagccaaag ctgagaaact ccagcaagaa ctagaaaata aatatgtcga
     1261 caatttcacc ttctacctcg ataagaaggc taaagaggaa aatacaaact ttacttcctt
     1321 tagcaacctg gtcaaagcta taaaccaaaa tccctctgga acctatcatt tagcggccag
     1381 cctgaatgct aacgaagtgg agcttggtcc tgatgaaaga tcctatatca aggacacctt
     1441 tactggtcgt ttaatcggtg aaaaagatgg caagaattat gctatctata atttgaaaaa
     1501 acctctgttt gaaaacttga gtggtgctac agtagaaaaa ctgagtctaa aaaatgttgc
     1561 tatttcaggg aaagatgata tcggttcact ggcaaatgaa gctcagaata atacaaaaat
     1621 taagcaagtt cacgtcgatg gtgttctggc tggtgaacgt ggtatcggtg gtttgctggc
     1681 taaggctgag caatcaagca tcacagagag cagtttcaag ggaagaatta tcaacactta
     1741 tgaaacgact gctgcctaca atatcggtgg tatggtcggt catttgacag gtgacaaggc
     1801 tttacttact aagtcaaaag cgacagtagc catttcatct aacacaaata cttcagatca
     1861 gactgtgggt ggacttgcag gcctagtaga ccgagatgca cagatccaag atagctatgc
     1921 tgaaggtgat atcaacaatg tcaagcactt tggtagagtc gctggagtgg caggcaattt
     1981 gtgggatcga acttctggtg atgttaggta tgctggaagt ttgaccaatg ttctcagcga
     2041 tgttaatgta accaacggaa atgccatcac tggttaccac tataacgaaa tgaaggtaaa
     2101 ggacacattc agcagcaagg ccaacagagt ctacaatgtc accttggtca aggatgaggt
     2161 cgtcagcaag gaatcctttg aagaaagagg aacaatgcta gatgcttctc aaattgcaag
     2221 caaaaaagca gaaatcaatc ctttcattct accaacagtg gagccacttt caacaagtgg
     2281 caaaaaagac agtgattttt ctaaggtggc ctattatcaa gctaagcgca acttgactta
     2341 taaaaacatt gaaaaattgc tacctttcta caacaaggca accatcgtca aatacggaaa
     2401 cctggtcaat gagaacagtc ttttatatca aaaagaactc ttgtcagcag tcatgatgaa
     2461 ggacaaccaa gtcatcacag acattgtttc taacaaacag actgcaaaca aactcttgct
     2521 tcactacaag gatgatttat ctgagaagct ggatctcaaa taccagaatg atttcgccaa
     2581 attagcagaa tatagtctgg gcaatactgg acttctctat acgccaaacc aattcctgta
     2641 tgaccaaacc tctatcatca agcaagtctt acctgactta caaaaggttg actatcattc
     2701 agaagccatc agaaagacgc tgggtatttc tccaaacgtc aagcaaactg agctctatct
     2761 agaagaccag ttcgccaaaa caaaacaaca actggaagac agtttgaaaa aactcttgtc
     2821 agcggatgct ggactggcta gtgctaaccc agtcactgaa ggttatcttg tagataaaat
     2881 caaacgcaac aaggaagcct tgctacttgg cttgacctat ctggaacggt ggtataactt
     2941 tagctatggt caggtgaatg tcaaagacct agttctgtac catttggact tctttggtaa
     3001 ggggaatgct tcaccattag atactctgat tgagttgggt aaatctggct ttaacaatct
     3061 tctagctaag aataatgtcg atacttatgg tatcagtctt gccagtcaac atggaacgac
     3121 agatttgttt agcacgctgg aacattaccg aaaagtcttt ttaccaaata caagcaataa
     3181 tgactggttt aaatcagaga ctaaggctta cattgtcgaa gaaaaatcca ctattgcaga
     3241 ggtcaaagcc aagcaaaagc aagcaggaac taagtactct atcggtgttt atgaccgaat
     3301 tactagtaat acttggaaat accgcaatat ggtcttgcct ctcctgacct tgccagagag
     3361 atccgtattt gtcatctcga ccatgtctag tctaggattt ggagcttatg atcgctaccg
     3421 cagtagtgac cataaagcgg gcaaggctct caatgatttt gttgaagaaa atgcgcgtga
     3481 aacagccaaa cgtcagcgag atcactacga ttattggtat cgtattttag atgaacagtc
     3541 acgcgaaaaa ctctatcgta cgattctcct ttatgatgcc tataagtttg gggatgatac
     3601 aacatcagga aaagctacag cggaggctaa gtttgatagc tccaatccag ctatgaagaa
     3661 cttctttgga ccagttggca ataaggtagt acacaaccag catggagcct acgctacagg
     3721 ggacggcgtc tactatatgt cttaccgcat gctggacaag gatggagcca ttacttatac
     3781 ccatgaaatg acccatgatt cggatcagga tatttacctt ggtggctatg gtcgaagaag
     3841 tggcttggga ccagagttct tcgcaaaagg cttattgcaa gcccctgacc aaccaagtga
     3901 cgcaaccatt accatcaatt ctatcttgaa acactcaaaa tcagatagta cagagggatc
     3961 ccgtcttcaa gtcttggatc cgacagagag attccaaaac gcagcagatc ttcagaacta
     4021 tgtccataac atgtttgacc ttatctacat gatggaatat ctcgaagggc agtcaatcgt
     4081 taataaacta agtgtttacc agaaaataga agctctcaga aaaatcgaga accaatatct
     4141 aacagatcct gctgatggaa atgatgttta tgctactaat gttgtgaaaa atttgactga
     4201 agatgaagct aaaaaattga cgagctttga tagtttgatt gacaataaca ttctttcggc
     4261 tcgtgagtat aaggctggca cttatgagag aaatggctac tttactataa aactctttgc
     4321 accaatcttt tcagcattga gcggtgagaa aggaactcca ggtgacctta tgggacgaag
     4381 gatagctttt gaacttctag ctgctaaagg atttaaagat ggtatggtac catatatttc
     4441 caaccaatac gaagaagatg ctaaacaaca agggcaaact atcaatcttt atggtaaaga
     4501 acggggcttg gtgaccgatg agcttgtttt gaaaaaggta tttgatggta agtataaaac
     4561 ttgggcagaa tttaagacag ctatgtacca agaacgggtg gatcagtttg gaaacttgaa
     4621 gcaggtgacc tttaaagatc cgacaaaacc atggccaaga tatggcacaa agactatcaa
     4681 taatgtggat gaattgcaga aactaatgga tgaagctgtt ttgcaggatg ctacaggtac
     4741 acgttggagt aattataatc cagaaacgga cagtgccgtc cataagttga agagagcaat
     4801 ctttaaagcc tatcttgacc aaacaaatga ttttagaagt tcaatttttg agaataaaaa
     4861 atagtgttta ctattaggaa ataaagttta aaaaggtgat gaagaacaaa ccaagattca
     4921 agcaggaatt cctcctgata atgaagtaag ttatgctctt atttatcagc aggaaactct
     4981 tcctgcaaca ggttcatcaa cttctgcgct tacagcttta ggcctattag ctgttggtag
     5041 tttagttctt ttggttcata atatgacggg aacagttttt tgctccctct gaaaagtcat
     5101 catttgatgg cttttttcta tatagggtaa aagatagggt aaaaggctat catcggacaa
     5161 aataaagaag gcatgatata atataaagta gatttctatg tcataaaaca agaactattt
     5221 ggacatcatt catttgaaaa ctctctatgt tcaaactata gtaaaataaa ataggggatc
     5281 taaatccttg ctacgaaagg aaaaaaactc aatggctact attcaatggt ttcctggtca
     5341 catgtctaaa gctcgtcgac aagtgcagga gaatttaaaa tttgttgatt ttgtgacgat
     5401 tttagttgat gcacgcttac ctctatctag tcaaaatcct atgttgatca agattgttgg
     5461 tgataaacca aaactcttga ttttaaacaa ggccgacttg gctgatccag caatgaccaa
     5521 ggaatggcgt cagtattttg aatcacaagg aatccagacg ctagctatca actccaaaga
     5581 gcaagtgact gtaaaagttg taacagatgc ggccaagaag ctcatggctg ataagattgc
     5641 tcgccagaaa gaacgtggga ttcagattga aaccttgcgt accatgatta tcgggattcc
     5701 aaacgctggt aaatcaactc tgatgaaccg tttggctggt aaaaagattg ctgttgttgg
     5761 aaacaagcca ggggtcacaa aaggtcaaca atggcttaaa accaataaag atctggaaat
     5821 cttggataca ccggggattc tctggcctaa gtttgaggat gaaactgttg cacttaagtt
     5881 ggcattgact ggagctatca aagaccagtt gcttcctatg gatgaggtta ccatttttgg
     5941 tatcaattat ttcaaagaac attatccaga aaagctggct gaacgcttca aacaaatgaa
     6001 aattgaagaa gaagcgcctg tgattattat ggatatgacc cgcgccctcg gtttccgtga
     6061 tgactatgac cgtttttaca gtctcttcgt gaaggaagtc cgtgatggca aactcggtaa
     6121 ctatacctta gatacattgg aagacctcga tggcaacgat taaagaaatc aaagaactcc
     6181 ttgtgacagt caaggagtta gaaagcccta tttttttaga tcttgaaaag gataatcgct
     6241 caggagttca aaaggaaatc agcaagcgta aaagagccat tcaagctgaa ttagatgaaa
     6301 atttgcgctt ggaatccatg ctttcttatg aaaaagaact ttataagcaa ggattgacct
     6361 taattgcagg tattgatgag gttggtcgtg gtcctcttgc tggtcctgta gtcgctgcgg
     6421 ccgttatttt acctaaaaat tgtaagatta aaggtctcaa cgacagcaag aaaattccta
     6481 aaaagaaaca tctggagatt ttccaagccg ttcaagacca agccttgtcg attggaattg
     6541 gtatcataga taatcaggtc atcgaccaag tcaatatcta tgaagcaacc aaactagcca
     6601 tgcaagaagc aatctcccag ctcagccctc aaccagagca ccttttgatt gatgccatga
     6661 aactggactt gcccatttca caaacctcca ttatcaaagg agatgccaac tccctctcta
     6721 tcgcagcagc atctatagta gccaaggtaa cacgtgatga attgatgaag gaatacgatc
     6781 agcagttccc tggctatgat ttcgctacta atgcaggata tggcacagct aaacatctgg
     6841 aaggcctcac aaaactagga gttaccccaa ttcaccgaac cagctttgaa cccgttaaat
     6901 cactggtttt aggtaaaaaa gaaagttaat tgaaaggaaa taccatggag gaacagtcgg
     6961 aaatagtccg ttctaagaaa gaatt
//
LOCUS       DAVAAL010000094         6964 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVAAL010000094 DAVAAL010000000
VERSION     DAVAAL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.095"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            18..1061
                     /locus_tag="VQM60_001555"
     CDS             18..1061
                     /locus_tag="VQM60_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET1081412.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYKVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            1125..2810
                     /gene="lpdA"
                     /locus_tag="VQM60_001556"
     CDS             1125..2810
                     /gene="lpdA"
                     /locus_tag="VQM60_001556"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET1081413.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            2874..3863
                     /locus_tag="VQM60_001557"
     CDS             2874..3863
                     /locus_tag="VQM60_001557"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET1081414.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAKINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(3936..5006)
                     /gene="xerS"
                     /locus_tag="VQM60_001558"
     CDS             complement(3936..5006)
                     /gene="xerS"
                     /locus_tag="VQM60_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET1081415.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(5500..>6964)
                     /locus_tag="VQM60_001559"
     CDS             complement(5500..>6964)
                     /locus_tag="VQM60_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET1081416.1"
                     /translation="IVGLIVGIIVGSFRFLIEKGFHLIQGVYQDQGYLVRNLFVLVLF
                     YILICWLSAKLTRSEKDIKGSGIPQVEAELKGLMSLNWWGILWKKYVLGILAIASGLM
                     LGREGPSIQLGAVGGKGIAKWLKSSPVEERSLIASGAAAGLAAAFNAPIAALLFVVEE
                     VYHHFSRFFWVSTLAASIVANFVSLLMFGLTPVLDMPDNIPPMTLDQYWIYLVMGIFL
                     GFSGFLYEKAVLNVGRVYDLIGQKIHLDRAYYPILAFILIIPVGIFLPQIIGGGNQLV
                     LSLTEQNFSFQVLLAYFLIRFIWSMISYGSGLPGGIFLPILALGSLLGALIGAICVNL
                     GLVSQEQFPIFVILGMSGYFGAISKAPLTAMILVTEMVGDIRNLMPLGLVTLVSYIIM
                     DLLKGTPVYEAMLEKMLPEEVSSEGEVTLIEIPVSDKIAGKQVHELNLPHNVLITTQV
                     HNGKSQTVNGSTRMYLGDMIHLVIPKSEIGKVKDLLL"
BASE COUNT         2186 a         1304 c         1454 g         2020 t
ORIGIN      
        1 atagaattgg agaggtcatg gctgatgaca agctaagagc gactcctgca gctagaaagt
       61 tagcggatga tttagggata aacctctacg acgtttctgg ctcaggtgca aacggtcgtg
      121 tccacaaaga agacgtggaa acttataaag acacaaacgt ggttcgcatt tcgccacttg
      181 caaaacgaat tgccctcgaa cataacattg cttggcagga aatccaagga accggtcatc
      241 gtggtaaaat catgaagaag gatgttttgg ccctgcttcc tgaaaatatc gaaaacgata
      301 gcatcaaatc tcctgctcag attgaaaaag tggaagaagt ccctgataac gtaacaccat
      361 atggtgaaat cgagcgtatt ccaatgacac caatgcgtaa ggttattgcc caacgcatgg
      421 ttgaatctta cttgactgcg ccaaccttca ccctcaacta taaagttgat atgactgaaa
      481 tgttggctct tcgtaagaag gttcttgagc caatcatgga agcaactggg aagaagacta
      541 ctgtaacaga ccttctttcg cttgcagttg ttaagactct tatgaaacac ccatacatca
      601 acgcttcatt gacagaagat ggcaagacta ttatcactca caactatgtc aatcttgcca
      661 tggcagttgg gatggataat ggattgatga cacctgtcgt ttacaatgct gagaaaatga
      721 gtctttcaga actggttgta gcctttaagg atgttattgg ccgtaccttg gatggtaaat
      781 tggctccaag tgagttacaa aattcaacat tcacaatcag taatttggga atgtttggtg
      841 ttcagtcctt tggtccgatt attaaccaac ccaactcagc tatccttggt gtcagttcga
      901 caatcgagaa gccagttgtc gtcaatggtg aaattgtgat tcgcccaatc atgagtttag
      961 gattaaccat tgaccaccgt gtcgtagatg gtatggctgg tgctaagttt atgaaggact
     1021 tgaaagagtt gattgaaacg ccaatctcaa tgttaattta agagcaagag tattcatttt
     1081 taagatatag ataaaggaag gaagacatgg ccttagaagt aattatgcca aaagccggcg
     1141 tggatatgac agaaggacaa atcgtccaat ggaataaaaa agtcggagaa tttgtaaaag
     1201 aaggagaaat ccttttggaa atcatgactg ataaagtcag catggaattg gaagccgaag
     1261 aagatgggta cttgattgcc attctcaaag gagatggtga aactgtccct gtaacggaag
     1321 ttatcggtta ccttggtgaa gaaagggaaa acatcccaac agctggagca gcatcgccag
     1381 aagctagccc tgtacctgta gcaagtacct caaacgatga tggtaagagc gatgatgctt
     1441 ttgatatcgt tgtgattggt ggaggtcctg ctggttatgt tgcagccatt aaagctgccc
     1501 aattcggcgg taaggttgcc cttgttgaga aatctgaact tggtggaacc tgcttaaacc
     1561 gtggatgtat tccaaccaag acctaccttc ataacgctga aattattgaa aatatcggtc
     1621 atgctgcaaa tcgtggtatc gtgattgaaa atcctaactt cactgttgat atggaaaaac
     1681 ttttagaaac taaatctaaa gttgttaata ctcttgttgg tggtgttgca ggacttcttc
     1741 gtagctacgg agttactgtt cataaaggaa ttggtacaat cactaaagac aagaacgtct
     1801 tggtaaatgg ttctgaattg cttgaaacca agaaaatcat tcttgctggt ggttcaaaag
     1861 tcagcaagat caacgtccct ggtatggaat ctccacttgt catgactagt gatgacattc
     1921 ttgaaatgaa cgaagtgcca gaaagccttg ttatcatcgg tggtggagtt gtcggtatcg
     1981 aactcggtca ggccttcatg acatttggtt caaaagtgac tgttatcgaa atgatggacc
     2041 gtatagttcc agctatggat gtggaagttt ctaagaacct tcgcttgatt cttgagcgta
     2101 aaggaatgac catcttgact ggtactaaac tgcaagaaat cattgaggaa aatggtcaac
     2161 ttcgtatcaa ggttgaagga aaagacgata tcatcgcaag caaagctctt ctttcaattg
     2221 gtcgtatgcc agaccttgaa ggtattggag aggttgagtt tgaattggat cgtggttgta
     2281 tcaaggtcaa cgaatacatg gaaacttcag ttccaggcat ttatgcacca ggtgacatca
     2341 acggtactaa gatgttggct cacgcagctt tccgcatggg tgaagtttcc gctgaaaatg
     2401 cacttaaagg aaatcatgca gttgccaaat tgaatttgac tcctgcagcc atctacactc
     2461 tccctgaagt agcagcagta ggtttgacag aagaacaagc ccgtgagaaa tacgatgttg
     2521 ccatcggtaa gtttaacttt gctgctaacg gtcgtgctat tgcatctgac gcagctcaag
     2581 gtttcgtaaa agttatcgct gataagaaat acggagaaat ccttggtgta cacattattg
     2641 gtcctgcagc cgcagaatta ataaacgaag catcaagcat catcgaaatg gaaatcactg
     2701 ttgaggaaat gctgaagacc atccacgggc acccaactta ctctgaagtg atgtacgaag
     2761 cgtttgcaga tgttctagga atggccatcc attcacctaa gaaaaaataa aacgaagata
     2821 gattagactg gaaggttatt tccagtttct atcatataaa gtaaagggat atcatgaaat
     2881 acattatcaa tcattcaaat gacactgctt ttaatattgc cttggaagaa tacgccttta
     2941 aacacctttt ggatgaggat caaatcttcc tactttggat taacaagcca tctatcattg
     3001 ttggtcgtca ccagaacact atcgaagaaa tcaaccgtga ttatgttcgc gaaaatggta
     3061 ttgaggtagt tcgccgtatt agtggtggtg gagctgttta ccacgattta aataacctca
     3121 actacacgat catctccaaa gaagatgaaa ataaggcatt tgacttcaag agcttctcaa
     3181 ctccggttat caataccctg gctcaacttg gggttaaagc tgagttcaca ggtcgtaatg
     3241 accttgagat tgatggtaag aagttctgtg gcaatgccca agcctatatc aacggccgta
     3301 tcatgcacca cggttgcttg ctctttgacg ttgatttgtc agtcctcgca aatgccctta
     3361 aggtttcaaa agataaattt gaatcaaaag gtgtgaaatc cgtccgtgcc cgtgtaacca
     3421 atattatcaa tgaattacca aaaaaaatca cagtcgaaaa attccgtgat ttactcttgg
     3481 aatacatgaa aaaagagtac ccagagatga ctgaatacgt cttttcagaa gaagaattgg
     3541 ccaaaatcaa tcacatcaag gatactaagt ttggaacttg ggactggaac tatggtaaat
     3601 cacctgaatt taacgtccgt cgtggaacca aattcactag tggtaaggtt gaagtctttg
     3661 ctaacgttac tgaatcaaaa atccaagaca tcaagattta tggtgacttc tttggtattg
     3721 aagacgttgc tgcagtagaa gatgtccttc gtggggtgaa atacgaacgc gaagatgttc
     3781 ttaaggcgct aaaaaccatt gatatcacac gctacttcgc tggtattagc cgtgaagaaa
     3841 tcgctgaagc ggtagttgga taaatcaaaa agaagttggc caactaacca acttcttttt
     3901 ttgatattta tttacataat ttaaaatacg taaaattata aactatctag agcattcttt
     3961 tgttcatcat taacaatatg ggtatagagg tcagtgactt gtgtgctggc atgtcctagt
     4021 tggtgactga ctaaaacttg tgatttagtc gcatcataga gcctagttgc tagcgtatgg
     4081 cgcagtttat ggggtgttac acgcacttta aaatcctctg agtatttagc aaccattttc
     4141 tcaacgctag aagcatcgat acgattagga acacctctgt agagagttaa aaaaagggct
     4201 gtatctgttt tttccgtttt atagcgttga ttccgaatgg ccagataatt ctctaaataa
     4261 ggtttagcaa aagcagcgac attgactgag tcacgtttgc caccttttcg agtaacatca
     4321 ataaccatca tttttagatt gagatctctt agatctagat taacagcttc agataagcga
     4381 acaccagatg ccaagagaag ggcaataatg gctaaatctc gttctttatt tttgttgaat
     4441 gatgagagag ctcgatttga aagctgttgt gggtgctctt gatcgatata agttagaaaa
     4501 ccttctgttt catcacctag aaagagtttt tgcttgatat tttcagctct ggcagcaagg
     4561 gtttctttct ttttcttggt tgaaactttt ttcattacat tacgatagaa ataaggttcc
     4621 ccctgatcgt tttcaacctc ctcggttaga tacttgtaaa gactagaaag tgctgataag
     4681 gttcgattga tagttgtctg tgaaacaccc tgttttgttg tattagcatt cagcaaggga
     4741 cgttcacgta gataaaggat aaaggattcc atgtctttct tagacatatt ttccaaaact
     4801 gataaaggaa tatcggatat tttatcagcg tttgaaatac cagactccaa aacccagctg
     4861 aaaaatcggt catattcctt aaggtattcg tacagggttg taaaactgta gggtacagcc
     4921 agcttagatt ggtagtattc cagaacatac cagggcatga gttgttttag tttgtcgatt
     4981 cgttccagta aaatctcacg tttcatgtat tttctccata agtaagtata ctagtagtat
     5041 agcatagact tgtatatttt tcaagaaaat tatagttttt cagaaagatg ttataggagt
     5101 ttttcaaagt gataaaaaga cttagaccta caaacaaaac agaataagga aaataataaa
     5161 aatctcaccc actaaacttc tgttaatact acatagctag aaattgattt gattgtcctg
     5221 attgttttat cctatttcta tttcatttca ctatataatt gctattgttt tctttttcat
     5281 gattttaatc ttactgtaca gatttgatac agtaaactcc tttctagctt tatgctttat
     5341 catttctaac ttaattatag tcttattttt aataaagttc aattatttat atgtaaaatt
     5401 cctcatcagt aaaaaataac cgatctcatt tctgagaatc ggttttctaa ataaatcaag
     5461 ctgatcattt acataacata aattatgtaa atgattgtac tacaacaaca aatctttgac
     5521 ttttccaatt tcactttttg gaataaccag gtgaatcata tcacccagat acattctggt
     5581 tgagccgtta actgtttggc tcttgccatt atggacttga gttgtgatga ggacgttgtg
     5641 tggtaagttg agttcatgaa cttgtttccc agcaatttta tcagaaactg gtatttcgat
     5701 aagtgtaact tctccttcgc tagatacttc ttctggaagc attttttcca gcatggcttc
     5761 atagactggc gtacctttga gcaaatccat gataatataa gaaacaagag tgacaagacc
     5821 aagtggcatg aggttgcgaa tatctcctac catctcagtt acgaggatca tagcggttaa
     5881 gggagctttt gatatggctc caaaatagcc actcattcct agaatgacaa atatagggaa
     5941 ttgctcttga ctgacaagtc caagattgac acagatagca ccaattaagg caccaagcaa
     6001 agaaccaaga gctaaaatgg ggaggaaaat tcctcctggc agtccacttc catagctaat
     6061 catactccaa ataaagcgga tcaaaaagta agctaataaa acttggaaac taaaattttg
     6121 ttcagttaaa gaaaggacaa gctgatttcc gccaccaatg atttgaggta agaagattcc
     6181 gactggtatg ataaggataa aagccaagat gggataataa gctctatcca aatggatttt
     6241 ttgaccaatc aagtcataaa ctcttccaac gtttaataca gctttctcat agagaaagcc
     6301 tgaaaatcca aggaaaattc ccatgacgag atatatccaa tactgatcta gggtcatggg
     6361 aggaatgtta tctggcatat ccaatactgg tgtcaaaccg aacatgagta gagacacaaa
     6421 gtttgctacg atgctggctg ctagagttga gacccagaaa aagcgcgaaa aatggtgata
     6481 gacttcttct acaacaaaga gaagtgctgc aataggagca ttaaaggctg cggctaaacc
     6541 tgctgcagct ccactggcaa tcaaggaacg ttcctctact ggactggatt tgagccactt
     6601 ggcaattcct ttaccaccaa ctgctccaag ttgaatactg ggtccctctc gacccagcat
     6661 gagtccactg gcaatagcaa gaatacctag cacatatttt ttccaaagaa tgccccacca
     6721 gttgagggac atgaggcctt tcagttcggc ttcgacttga ggaattcctg agcctttaat
     6781 atctttttct gaccgtgtta gtttggcact gagccaacag atgagtatat aaaacaaaac
     6841 cagtacaaaa agattgcgta ctaagtaccc ttgatcttga taaactcctt gtatcaggtg
     6901 gaagcccttt tcaattaaga aacgaaagga tccgacgata attccaacga tgaggccgac
     6961 aatg
//
LOCUS       DAVAAL010000095         6925 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVAAL010000095 DAVAAL010000000
VERSION     DAVAAL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.096"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(39..2036)
                     /gene="metG"
                     /locus_tag="VQM60_001560"
     CDS             complement(39..2036)
                     /gene="metG"
                     /locus_tag="VQM60_001560"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET1081417.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2160..3419)
                     /locus_tag="VQM60_001561"
     CDS             complement(2160..3419)
                     /locus_tag="VQM60_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1081418.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3421..4122)
                     /locus_tag="VQM60_001562"
     CDS             complement(3421..4122)
                     /locus_tag="VQM60_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081419.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4106..5305)
                     /locus_tag="VQM60_001563"
     CDS             complement(4106..5305)
                     /locus_tag="VQM60_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET1081420.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPATGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KTDEATN"
     gene            5591..>6925
                     /gene="gor"
                     /locus_tag="VQM60_001564"
     CDS             5591..>6925
                     /gene="gor"
                     /locus_tag="VQM60_001564"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET1081421.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFV"
BASE COUNT         2007 a         1545 c         1310 g         2063 t
ORIGIN      
        1 aaaaaaacag aactagcctg aactagtcct gtctactttt acccaatcac acttccattt
       61 ggtacagctg gatcaactgt gagaagggtt aatttgccat catgttcagc tgagagaatc
      121 ataccctggc tgacatattt tttcatcatt ttacgtggtt tgaggttagc aacgatttga
      181 actttcttgc cgaccaattc ttgttcattt ggatagtatt ttgcaattcc tgaaagaatc
      241 tgacgatctt ctccatcacc agcatccaag cggaattgaa gcaacttatc tgaaccttct
      301 actttagaca cttctttgac ttctgccaca cggatttcaa ccttgtcaaa gtcttcaaac
      361 ttgatttcat ccttgtttag tttgagctca acttcgtccg gattccattc tttttcgact
      421 gctggtttat tgccttccat ttgttccttg atataggcga tttcttcttc catatttaga
      481 cgtggaaaga taggtgttcc tttggcaact acagtcacat ctgctgggaa gtcagccaaa
      541 ctcaagtttt caagactaga aacttcttcc aaaccaagtt gagtcaaaac tgcacgacta
      601 gtttccatca taaatggttc aatcaagtga gcaactacac gaatgctggc tgccaagtgg
      661 ctcatgacac ttgccaattg gtcacgaaga gcttcatcct tgtccaagac ccatggtgca
      721 gtctcatcga tgtatttatt ggtacgagag atcagagtcc agactgcttc aagcgcacgt
      781 ggatagtcaa ctgcttccat gtgtgtatgg aagtctgcga ttgattgttc tgcaacctca
      841 gcaagaacat gatcaaattc agtcacacct tctacatagg cagggatttg tccatcaaag
      901 tacttattaa tcatggaaac cgtacggtta aggaggttcc caaggtcatt agccaattca
      961 tagttgatac ggccgacata gtcttcagga gtaaaggttc cgtctgaacc aactggaagg
     1021 ttacgcatga ggtagtaacg aagtggatct agtccataac gctctaccaa catttcaggg
     1081 taaacgacat tcccttttga cttagacatt tttccgtctt tcatgacaaa ccaaccatgg
     1141 gcaatcaaac gatcaggtaa tttaacatcc aacatcataa gaaggattgg ccagtagata
     1201 gagtggaagc gaaggatatc ttttcctacc atatggaaga ctgttccatt ccagaacttg
     1261 tcaaagttac catgttcgtc ttgagcgtag ccaagagctg tcgcatagtt aagaagggca
     1321 tcaatccaaa cgtagacaac gtgttttgga tttgatggga caggcactcc ccatgtaaag
     1381 gttgtacgag ataccgccaa atcttccaaa cctggctcga tgaagttgcg tagcatttca
     1441 ttaaggcgac catctggcgt gataaattca ggatgagctt tgaaaaattc gaccaaacgg
     1501 tcttggtatt tgctaaggcg aaggaagtat gattcttcag aaacccattc aacctcatga
     1561 cctgatggag caataccacc agtcacattt ccagcttcat cacggaaaac ttctgccagc
     1621 tggctttctg taaagaattc ttcgtctgat actgaatacc aaccagagta ttcacccaag
     1681 tagatatcat cttgagcaag taagcgttca aagacctgtg caacaacttt ttcatggtag
     1741 tcatcagttg tacggataaa tttatcgtat gagatatcta gtaattgcca gagttcttta
     1801 actccaaccg ccattccatc aacataggct tgaggtgtaa taccagcttc ttccgctttc
     1861 tgctggattt tctgaccatg ttcatcaaga cctgtcagat aaaatacatc gtagcccatc
     1921 aggcgtttgt aacgtgctag gacatcacat gcgatagttg tgtaggcaga accgatatga
     1981 agtttcccag atggatagta aatcggcgtt gtaatataaa aattcttttc agacataatt
     2041 tttcctttcc aggcaaatga aacctgtttt tctaacactt cattatatca catttttaat
     2101 gaatttcgat agggaaatcc atacaaaaac aagatagacg agtgtccatc ttgttgatct
     2161 cattcataac gaagggcttc aattggatca agtttcgatg ccttgttggc tggcaagact
     2221 ccaaaaatca taccaacact agccgaaact gcaagactaa atagggcaac tgggattgat
     2281 actccaactt ctataccttc tattaaacct tgcagtaaca aacctgctaa ggcagttaaa
     2341 ccacttgcaa ttgtcaagcc aattaagcca cctaacaagg tcaaaatcat ggattcaatc
     2401 aaaaactgaa ttaaaatatt ggcacgtgtt gcgcccaaag ccttacgaag accaatctca
     2461 cgagtgcgct ctgtcaccga aaccagcatg atgttcatga caccagttcc tccaacaaag
     2521 agagaaatcc ctgcgatgga actaataatc gtcgtcataa aactaaacga ttgttgaatt
     2581 tctgcaaata caacggactc atctgccacc tggtattctc cctgttgtaa gcctgcaagc
     2641 tctgtcattt ttcgtgccaa ttctggaccc agagttgggg ttaaactggt atcattcact
     2701 cgaaagacaa tattagctat ttcatctaca ttaaaattcg cagcaaggga gatattggta
     2761 gtaataggca agccaccaaa cccatatatt tttgatcttt tagcctccgg actagtataa
     2821 accccaatga cccggtaact aaatccattg acttctacaa ccttgttaat agcctcttga
     2881 ggagattcaa ataaactaat ggacaattcc tcatctagca aaatgacact tgcaaactct
     2941 ttgaaatctt gctctctcag actacgacct gcaataattt cattcttaac agcgtccatg
     3001 taagttctgt ttccacctgt caaattagca ttctcaacct ttttatcttg ataggtcaag
     3061 atggcattcg ttgaattggt tacatagtaa ctatccactc ccttcagttt agctgcctct
     3121 tgaacccagg attcttgcgg ttttggcggt tcaacaggaa cttcctcttc ctttccagaa
     3181 accgtaaaag ctgattgttt ctgagtaaaa gacccatctt tacttttttt aggagagaaa
     3241 aagacgctaa tatttttctg agatttagtc atatctttat tgacttgacg agatagggaa
     3301 tcacccaaag ccataatcac aacaactgat gaaacaccga taataatccc aatcatagta
     3361 agcaaagaac gcatcttgtg agccatgata gatgaaaagg caaatttcag attctgcatc
     3421 ttagttttcc tcctttccta actgagcact gtcagacgaa atgaccccat cccgaatgac
     3481 aatctgacgt ttggcatagg cagcaatctc aggctcatgc gttaccatga taatggtttt
     3541 tccttcttta ttcaaatcaa ccaataattg cataatttgg ttacctgttt tggtatccaa
     3601 ggctcctgtc ggttcatccg ctaggataat agaaggattg tttaccaagg cacgcgcaat
     3661 ggctacacgt tgcttttgac caccagataa ttctgaaggt aaatggtgac tacgttctgt
     3721 caattcaacc ttgtctaaat attcctcagc caacttgcga cgttttgaag acgaaactcc
     3781 tgcgtaaatc aagggcaatt ctacattttg cagagcattg agcttcgata gaagaaagaa
     3841 ctgctgaaag acaaaaccga tttgttggtt acggacctta gccagttgtt tttcaccaag
     3901 cccagccact tcttgacctt caagataata ttctccactg gttggtgtat ccaacatgcc
     3961 aatcgtattc atcagagtgg acttaccaga cccagatggt cccatgatgg ctacaaattc
     4021 accctcattc acttctagat tgatattttt gagaacctgc aattcttggt caccattacg
     4081 gtagcttctg aagatatttt ttagactaat tagttgcttc atcagtcttc acctcttttc
     4141 cttcttccaa ggaagatgtt ggattactga tgaccttagc accattcgtt aaaccagaag
     4201 tgatttcttg attttctgcg tcagcatttc ccaatgaaac ctcaactttt ttagcctttt
     4261 gttgttcatc cacaatccag acataatttt tactatcatc cattactaga ctgctaacag
     4321 gaacaagaat agccttagtt ttgcttttaa cctcaatgtt gacagaaaaa ccctgtttca
     4381 aatcaccaac ctcgcctgtc acatcaatag tataagggta tttagaacct gtattattcc
     4441 cggttgctgg actagctgct tcaccattgt ttttaggata gtcagaaata tagcttaatt
     4501 tcccagtcca ttttttatca ggatacactt tagaagtaaa gcttacttct tgacctacag
     4561 aaaggttggc tagattgtac tcagacaatt ctcccttgac ttgtaaattt tcattgctga
     4621 caatatgaac cataacttga ctcgcccctg ttggagattt agaaacattg ctattgactt
     4681 cgactacagt tccctctagg gtactgagaa cagttgttgc atccaattga ctttgagcct
     4741 tgcttaattg cgctgcagca tctgcacgcg catcacgggc atcacccaat tgagcatcaa
     4801 tagaagcaac agaatttcca gccactggag ttgggctttg caccgttgca tcttctcctc
     4861 ctactggcgc tggtaactgt ggagcctgag ctgaagcggc ttcatttcgt gcttgattga
     4921 gttcattgat atgacgatct gccttagcta ctgctcgact agctgaatca taggccgcct
     4981 gcgcttctga actactgtac ttgactaaag cctgcccttc gctgacctta tcgcccacag
     5041 aaacaaggat ttcatctaaa tcacccttac tagcatcaaa ataaacatat tgttcatttt
     5101 ttgctgttac tgtccctgac aataaaacag aggatgccac gcttccttcc ttggcaacaa
     5161 caagatgagt aggctcatct tttacagcag tctgagaagg ttgtctaaaa agtaaaatcc
     5221 ccccggcacc caatacaact acactcgcag caccgattgc tgcatacagt tgccactttt
     5281 tagctttacc attctttttc ttcataatga aactcctttt cttttttaca atactttgct
     5341 attataccaa atttccctcc agcaaacaat gcagttcaga tggaaataat ggcagttcag
     5401 gattaaacaa tcgttcggaa ttttgctttt ctattgtact agatatataa tacactatct
     5461 tgacttattt ggcaagcttt tttgcaattt tttgtgcgta gcagattttt gcaatcaaat
     5521 caaaaaaaga ataaaatata actatcaaaa aagacactct actatttaaa agattacaaa
     5581 ggagttttca atgagagaat atgatatcat tgctatcggt ggaggtagcg gaggaatcgc
     5641 taccatgaac cgtgctggtg aacatggagc caaagcagcc gttattgagg aaaagaaatt
     5701 aggtggaacc tgtgtcaacg tcggttgtgt tcctaaaaaa atcatgtggt acggggcgca
     5761 aatcgctgag actttccatc aatttggaga agactacggc tttaagacta ctgatcttaa
     5821 ctttgacttt gcaaccctac gtcgcaatcg tgaagcctac attgatcgcg ctcgttcttc
     5881 ttatgatggt agttttaaac gcaacggtgt agacttgatt gaaggtcatg ctgaatttgt
     5941 agattctcat actgtaagcg taaatggtga actgattcgt gctaaacata tcgtgattgc
     6001 tacaggtgcc catccaagta ttcctaatat tcctggtgct gagctaggtg gctcttctga
     6061 tgatgtattt gcctgggaag aacttccaga gtcaattgcc attctaggcg ctggttatat
     6121 cgccgttgaa ttagctggcg tactccacac ttttggtgtc aagacagatc tctttgttcg
     6181 ccgcgatcgt cctttacgtg gttttgattc ctacatcgtt gaaggtttgg tcaaggaaat
     6241 ggaaagaaca aacttaccac ttcacactca caaagtccct gtcaagttag aaaaaactac
     6301 tgacggcatt accattcatt tcgaagatgg tactagtcac acagctagcc aagttatctg
     6361 ggctacaggt cgccgtccaa acgttaaggg cttgcaactt gaaaaagctg gagtgactct
     6421 gaacgaacgt ggctttatcc aagtggatga ataccaaaat actgttgttg agggaatcta
     6481 tgctctaggt gatgtaacgg gcgagaaaga actgactcca gttgcaatca aggccggacg
     6541 taccctatct gaacgtctct ttaacggaaa aactactgca aaaatggatt actcaactat
     6601 tccaactgtt gtcttttcac accctgctat cggaactgtt ggtttgacag aagagcaagc
     6661 tattaaagaa tacggtcaag accaaatcaa ggtttataaa tcaagctttg catctatgta
     6721 ctctgcttgc actcgcaacc gtcaagaatc ccgtttcaaa ctcataacag ctggttcaga
     6781 agaaaaagtt gtcggacttc atggaattgg ctacggcgtt gatgaaatga ttcagggatt
     6841 tgccgttgct atcaaaatgg gagcaaccaa ggctgacttt gatgcaactg tggcaattca
     6901 cccaactgca tctgaagaat ttgta
//
LOCUS       DAVAAL010000096         6874 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVAAL010000096 DAVAAL010000000
VERSION     DAVAAL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.097"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(1..253)
                     /locus_tag="VQM60_001565"
                     /pseudo
     CDS             complement(1..253)
                     /locus_tag="VQM60_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            451..2217
                     /gene="patB"
                     /locus_tag="VQM60_001566"
     CDS             451..2217
                     /gene="patB"
                     /locus_tag="VQM60_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET1081422.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDISISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2356..3045
                     /locus_tag="VQM60_001567"
     CDS             2356..3045
                     /locus_tag="VQM60_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET1081423.1"
                     /translation="MARTVVGVTANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            3049..3228
                     /locus_tag="VQM60_001568"
     CDS             3049..3228
                     /locus_tag="VQM60_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081424.1"
                     /translation="MEYLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3215..4564
                     /locus_tag="VQM60_001569"
     CDS             3215..4564
                     /locus_tag="VQM60_001569"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET1081425.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADSVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWETFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4688..6148
                     /locus_tag="VQM60_001570"
     CDS             4688..6148
                     /locus_tag="VQM60_001570"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET1081426.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6162..6596
                     /locus_tag="VQM60_001571"
     CDS             6162..6596
                     /locus_tag="VQM60_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET1081427.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
     gene            6717..6788
                     /locus_tag="VQM60_001572"
     tRNA            6717..6788
                     /locus_tag="VQM60_001572"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6750..6752,aa:Glu,seq:ttc)
BASE COUNT         2176 a         1313 c         1476 g         1909 t
ORIGIN      
        1 gaggggaact ccgtttgacc attcttcctg ctaaatcaat ctgatctgac tgataaatag
       61 aagaatccag tccagcgaaa gcttgtaatt gagcaggatt atcaaaggca tgaatatttc
      121 gaatctcagc taaaatgacc gcccctaaac gatccccaat cccagtaacc gtcgtgatga
      181 ccgagttgaa ctcagccatc aagtcattga cacatgtttc cgccttgtca atgagcctct
      241 tgtaatgttt gatgttttca ttacacgaga taaaacgtct atgcgttatc aaactcatta
      301 ccaattaaaa caaaaagctg tggttagatc ctttcggaaa ttgtcaagcg attggaggaa
      361 atgaactaat ccacagcggc ttattccaag tataccactt gggctttggc agtagctaac
      421 tgcgctaaat ataatataag gaggagtaaa atgaagacag ttcaattttt ttggcattat
      481 tttaaggtct acaagttctc atttgtagtt gtcatcctga tgattgttct ggcgactttt
      541 gcccaagccc tctttccagt cttttctgga caagcggtga cgcagctagc caatttagtt
      601 caagcttatc aaaatggcaa tccagaactt gtatggcaaa gcctatcagg aatcatggtc
      661 aatcttggcc tgctggtttt ggttctattt atctctagtg taatatacat gtgtctcatg
      721 acgcgcgtga ttgcagaatc gaccaacgag atgcgcaaag gcctctttgg taagcttgct
      781 cagttgacgg tttctttctt tgaccgtcga caagatggcg atatcctgtc tcattttacc
      841 agtgatttgg ataatatcct ccaagccttt aacgaaagct tgattcaggt catgagcaat
      901 attgttttat acattggtct gattcttgtc atgttttcga gaaatgtgac gctggctctc
      961 atcaccattg ccagcacccc attggctttc cttatgctga ttttcatcgt gaaaatggca
     1021 cgcaaataca ccaacctcca gcagaaagag gtagggaagc tcaacgccta tatggatgag
     1081 agcatctcag gccaaaaagc cgtgattgtg caaggaattc aagaggatat gatggcagga
     1141 tttcttgaac aaaatgagcg cgtgcgcaag gcaaccttta aaggaagaat gttctcagga
     1201 attcttttcc ctgtcatgaa tgggatgagc ctgattaata cagccatcgt catctttgct
     1261 ggttcggctg tacttttgaa tgataagtct attgaaacaa gtacagccct aggtttgatt
     1321 gttatgtttg cacaattttc acagcagtac taccagccta ttatccaagt tgcagcgagt
     1381 tggggaagcc ttcagttggc ctttactgga gctgaacgaa ttcaggaaat gtttgatgca
     1441 gaggaggaaa tccgacctga aaaggctcca accttcacta agttgcaaga aagtgttgaa
     1501 atcagtcata tcgatttttc atacttgcct gataaaccta ttttgaaaga tatcagcatt
     1561 tctgccccta aaggccagat gacagcagtt gttgggccga caggttcagg aaaaacgact
     1621 attatgaacc tcatcaatcg cttttatgat gttgatgctg gtggtattta ttttgatggt
     1681 aaagacattc gtggctatga tttagatagt cttagaagca aggtgggaat tgtattgcaa
     1741 gattcggtct tgtttagcgg aacgattcga gacaatatcc gatttggtgt gccagatgct
     1801 agtcaggaaa tggttgaggt agcagcaaaa gcaacccaca ttcacgacta tatcgaaagt
     1861 ttgcctgata agtacgatac tcttattgat gatgaccaga gcatcttttc aacagggcag
     1921 aagcaattga tttcaatcgc tcgaaccctg atgacagatc cagaagttct cattctcgat
     1981 gaagcaactt caaacgtaga tacggtgaca gaaagcaaga ttcagcatgc catggaggtg
     2041 gttgtagcag gtagaactag tttcgtcatt gcccaccgct tgaaaaccat tctcaatgca
     2101 gatcagatta ttgtccttaa agatggagaa gtcattgaac gtggtaacca ccatgaactt
     2161 ttgaagctag gtggctttta ttcagaactc tatcacaatc aatttgtttt cgaataagaa
     2221 agaagttgtc ctatgtgggc agctttttct tgtccataaa aaatgtttat cacagcctta
     2281 aaaaaaacat attagacgaa agtcattttg agtgatatga taggactatc gttagcattc
     2341 gaaaggagag gcatcatggc tagaacggtt gtaggagtta ctgcaaatct atgtcccgta
     2401 gacgcagaag gcaaaatcat tcattcatct gtatcttgta gattcgcaga gatcattcgt
     2461 caagtcggtg gtctcccttt agtcattcct gttggtgatg agtcagttgt acgtgattat
     2521 gtggaaatga ttgacaaact cattttgaca ggaggccaaa atgttcatcc tcagttttat
     2581 ggagagaaaa agaccgtcga gagcgatgat tacaatctgg tccgtgacga atttgaattg
     2641 gcactcttga aggaagcgct tcgtcagaat aaaccaatta tggcaatctg tcgcggtgtc
     2701 caacttgtca atgttgcctt tggtggaacc ctcaatcaag aaatcgaagg tcactggcaa
     2761 ggactacctt tcgggacatc tcactctatt gagacagtag aaggaagcgt ggtggctaag
     2821 ctatttggga aagaaagtca ggtcaattca gtccatcgtc aaagcattaa agatttggca
     2881 cccaatttcc gtgtaactgc tattgattca agagaccaaa ctattgaagc gattgagtct
     2941 atcgacgagc accgcattat cggtttacag tggcatccag agtttctggt taatgaagaa
     3001 gatggcaatt tagaattatt tgagtattta ttgaatgaac tgtaacgatt ggaatatctg
     3061 gtcgttcttt cttttatttt ttcaaaattt ttagaatgtg gtattttacg caaacgtttg
     3121 aattctgata aaaattggag aaattcgaca aaaaaacttg aaaaaaacga aggtaagcgt
     3181 tatgatagaa aagaagaaat attggaggaa gaacatgtca catattaaat ttgattattc
     3241 aaaagtttta gacaaatttg ttgcaccaca tgaagtggaa tacatgcaat cacaagtaac
     3301 agcagcagat gaattgatcc gtaaaggaac tggtgctggt agcgacttct tgggatggtt
     3361 ggaccttcct gaaaaatacg accgcgaaga atttgaccgc atcttgaaag ctgctgagca
     3421 aatcaagtca gacagcgatg ttttggttgt gatcggtatt ggtggatctt accttggtgc
     3481 aaaagcagca atcgacttct tgaatcacca ttttgctaac ttgcaaacaa aagaagaacg
     3541 caaagctcca caaatccttt acgcaggaaa ctcaatctca tctacttacc ttgctgactc
     3601 ggtagagtat gttgcagaca aagacttctc agtaaacgta atttctaaat caggtacaac
     3661 aactgaacca gcgattgctt tccgtgtctt taaagaactc ttggttaaga aatacggtca
     3721 agaagaagct aacaaacgta tctatgcaac aactgaccgc caaaagggtg ctgttaaggt
     3781 tgaagcagac gctaacggtt gggaaacatt tgttgttcca gatgatatcg gtggacgctt
     3841 ctcagtattg acagccgttg gtttgcttcc aatcgcagca tcaggagctg acatcaaagc
     3901 tcttatggaa ggtgcgaatg cagctcgcaa agactacact tcagacaaaa tctctgaaaa
     3961 cgaagcttac caatacgcag ctgttcgtaa catcctttat cgtaaaggct atgcaactga
     4021 gatcttggta aactatgagc catcacttca atacttctca gaatggtgga aacaattggc
     4081 tggtgaatca gaaggaaaag accaaaaagg tatctaccca acttcagcca acttctcaac
     4141 tgacttgcac tcacttggtc aatttatcca agaaggaact cgtatcatgt ttgaaacagt
     4201 tgtccgtgtt gacaaacctc gtaaaaacgt gcttattcct actttggaag aagaccttga
     4261 cggacttggt taccttcaag gaaaagacgt tgactttgta aacaaaaaag caactgacgg
     4321 tgttcttctt gcccacacag atggtgatgt accaaacatg tatgtgactc ttccagagca
     4381 agacgctttc actcttggtt acactatcta cttcttcgaa ttggcaattg ccctttcagg
     4441 ttacttgaat gctatcaacc catttgacca accaggtgtt gaagcttata aacgtaacat
     4501 gtttgccctt cttggaaaac caggatttga agaattgagc aaagaactta acgcacgtct
     4561 ataatagaag aaaagagtgg tttacccact ctttttactc tctttatcca tagaaattgg
     4621 actcagtcaa gacttgtgat ataatataga aagcaaaaag gcagatgcct agataatagg
     4681 agaaactatg tcaaaagata tccgcgtacg ttacgcacca agtccaacag gactactaca
     4741 catcggaaat gctcgtacag cattgtttaa ttacttgtat gcgcgccatc atggtggaac
     4801 atttctcatc cgtatcgaag atactgaccg taaacgccat gtcgaggatg gtgaacgttc
     4861 acaacttgaa aaccttcgct ggttaggcat ggattgggat gaaagtccag aatcacatga
     4921 gaattatcgc cagtctgagc gtttagactt gtatcaaaaa tacattgacc aactattagc
     4981 tgaaggaaaa gcctataaat cttacgttac agaagaagag ttggcagctg aacgcgaacg
     5041 ccaagaagta gctggcgaaa caccacgcta catcaatgaa taccttggta tgagtgaaga
     5101 agaaaaagca gcttacatcg cagaacgtga agcagcaggg atcatcccaa ctgttcgttt
     5161 ggctgtcaat gagtcaggta tctacaagtg gcatgatatg gtcaaaggcg atatcgaatt
     5221 tgaaggtggc aatatcggtg gtgactgggt tatccaaaag aaagacggtt acccaactta
     5281 caactttgcc gttgttatcg atgaccacga tatgcaaatc tctcatgtta tccgtggaga
     5341 tgaccatatt gctaatacac caaaacagct tatggtctat gaagctcttg gttgggaagc
     5401 tccagagttc ggtcacatga ccttgattat caactctgag actggtaaga agttatctaa
     5461 acgtgatact aatacacttc aatttatcga agattaccga aagaaaggtt accttccaga
     5521 agcagtcttt aactttattg ctcttcttgg ttggaaccca ggtggtgaag atgagatttt
     5581 ctctcgtgaa gaactcatta aactttttga tgaaaaccgc ctcagcaagt caccagcagc
     5641 ctttgatcag aaaaaactag actggatgag caatgattat atcaagaatg cagaccttga
     5701 aaccatcttt gaaatggcaa aaccattctt agaggaagca ggccgtttga ctgacaaggc
     5761 tgaaaaatta gttgagctct ataaaccaca aatgaaatca gtagatgaga ttatcccatt
     5821 gacagatctt ttcttctcag atttcccaga attgacagaa gcagagcgcg aagtcatgac
     5881 gggtgaaaca gttccaacag ttcttgaagc attcaaagca aaacttgaag cgatgacaga
     5941 tgataaattt gtgacagaaa atatcttccc acaaattaaa gcagttcaaa aagaaacagg
     6001 tattaaaggg aaaaatcttt tcatgcctat tcgtatcgca gtttcaggcg aaatgcatgg
     6061 gccagaatta ccagatacaa ttttcttgct tggacgtgaa aaatcaattc agcatatcga
     6121 aaacatgcta aaagaaatct ctaaataaag aggatacaat aatggacatc tgggaaaaga
     6181 tgtacgaaga agcacagaaa ctatacaatc cacatgaagt atcagacttt gtttatgcca
     6241 atcatgttgt agctgcagta gaagcagaag atggacaaat atttacagga ttctgtatgg
     6301 agggaacctg tggtgttttc catctctgcg cagaacgggc ggccctcttc aatatgtacc
     6361 aattttcagg acaaactaag gttaagaaag tattagcctt tcgagacaaa ccaccttatg
     6421 gtggaagttc agccatgcct tgcggtgctt gtagagaatt ccttttagaa cttaatgctg
     6481 aaaataaaga tgcagaattc atgatggact ataatataag aaaaacagtt aaagtcgcag
     6541 aactaatccc ttattggtgg ggagaagaac gtgcttctaa gtttaatgag cgatagaaga
     6601 gtcagtataa ttttggctct ttttactata agttgaaaaa ataaaaaaaa ttagaaaaag
     6661 tagttgacaa agtttgaaaa gactgtataa tagtaagagt tgaaaataac aactcaggtc
     6721 cgttggtcaa ggggttaaga caccgccttt tcacggcggt aacacgggtt cgaatcccgt
     6781 acggactatg gtatgttgcg gttggaacac ttgatgaaaa aaagtttaaa aaaagcttaa
     6841 aaatcttcaa aaaagtgttg acaagtgaaa gtgg
//
LOCUS       DAVAAL010000097         6753 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVAAL010000097 DAVAAL010000000
VERSION     DAVAAL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.098"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..931
                     /locus_tag="VQM60_001573"
     CDS             <1..931
                     /locus_tag="VQM60_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET1081428.1"
                     /translation="EKSIASIQKQTYQNLEIILVDDGATDESGRLCDSIAEQDDRVSV
                     LHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQSLYEQLVQEDADVSSCGVM
                     NVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTICNKLIKRQIATALSFPKGL
                     IYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKPYAEKDLAYIDIYQKFYNEV
                     VKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYSQIHRFLKGHAFAISRNPIF
                     RKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            947..2380
                     /locus_tag="VQM60_001574"
     CDS             947..2380
                     /locus_tag="VQM60_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET1081429.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLVVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            2409..3443
                     /locus_tag="VQM60_001575"
     CDS             2409..3443
                     /locus_tag="VQM60_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET1081430.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            3643..4380
                     /gene="mecA"
                     /locus_tag="VQM60_001576"
     CDS             3643..4380
                     /gene="mecA"
                     /locus_tag="VQM60_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET1081431.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            4531..5817
                     /locus_tag="VQM60_001577"
     CDS             4531..5817
                     /locus_tag="VQM60_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET1081432.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDHDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            5819..6688
                     /locus_tag="VQM60_001578"
     CDS             5819..6688
                     /locus_tag="VQM60_001578"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET1081433.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPIIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
BASE COUNT         2056 a         1163 c         1431 g         2103 t
ORIGIN      
        1 agaaaaatcg atagcttcca ttcagaagca gacctatcaa aatctggaaa ttattcttgt
       61 tgatgatggt gcaacagatg aaagtggtcg cttgtgtgat tcaatcgctg aacaagatga
      121 cagggtgtca gtgcttcata aaaagaacga aggattgtcg caagcacgaa atgatgggat
      181 gaagcaggct cacggggatt atctgatttt tattgactca gatgattata tccatccaga
      241 aatgattcag agcttatatg agcaattagt tcaagaagat gcggatgttt caagctgtgg
      301 tgtcatgaat gtctatgcta atgatgaaag cccacagtca gccaatcagg atgactattt
      361 tgtctgtgat tctcaaacat ttctaaagga atacctcata ggtgaaaaaa tacctgggac
      421 gatttgcaat aagctaatca agagacagat tgcaactgcc ctatcctttc ctaaggggtt
      481 gatttacgaa gatgcctatt accattttga tttaatcaag ttggccaaga agtatgtggt
      541 taatactaaa ccctattatt actatttcca tagaggggat agtattacga ccaaacccta
      601 tgcagagaag gatttagcct atattgatat ctaccaaaag ttttataatg aagttgtgaa
      661 aaactatcct gacttgaaag aggtcgcttt tttcagattg gcctatgccc acttctttat
      721 tctggataag atgttgctag atgatcagta taaacagttt gaagcctatt ctcagattca
      781 tcgtttttta aaaggccatg cctttgctat ttctaggaat ccaattttcc gtaaggggag
      841 aagaattagt gctttggccc tattcataaa tatttcctta tatcgattct tattactgaa
      901 aaatattgaa aaatctaaaa aattacatta gaacgggggt gaccaagtga ttgatgggaa
      961 acgattatta tttagtttga ccatagtcag ttatgccttg acgctagtaa gtggaattgt
     1021 gtatctgttt aataataata atgttagctt actttctact ttattgttct tgttggttag
     1081 tagcttaatt gcttgttgga atgatatcaa gtattactta atccatttta ttttctattt
     1141 aaccattttt gtatttctgg tatcaagacc gaccattgat tattttaggg atggtgcttt
     1201 ggatacctat catccaatag cctatcgttt tgcctttata gttgtcatgg tttcgattct
     1261 gggcttgacc acaggaggca ttctggctcg ttacttcata gctaggaaga aaataaaagt
     1321 agcaaatata ggaaattctc taaaagaggt ttatatcaag cggttacgct ttgtatcact
     1381 agtagttttt cttctaacct atcctttcta tttcattcgg ttatttgaac ggctcttgta
     1441 tcgtttgcag acttcctact atgcctacta tgcaaatttt gaaagtaaac tgccttattt
     1501 tacctacatt ttgtctacct ttacggtcta tgcaatgtgt atgtatctgg caaccaagcc
     1561 aaagaaattg caggccacag cagtgcttgt ctcctttatt gcagctaata ctattcattt
     1621 ggcaattggg acacgaaatc cctttatttt aagtatttta tttgcttttg tttattactt
     1681 tatgcgggag caaactgaaa aaggaaaatg gattgggttt aaagaaaagt tagcgatttt
     1741 tgtaggttct cctattctca tgttagcgat gggagtactc aattatgtac gggataatgt
     1801 ccaagtttcc catacaggtt tctgggatat cttacttgac tttatctata aacaagggac
     1861 tagttttggt gttctggctc gaggttttct atttaacagt agcctccctt accgagattt
     1921 ccgtaatttt acttttggtc ctgttcttga ttattttgca agggggagtt tgggagccat
     1981 tttcggagga aaagcctttg aacatacaac caatagtgtg gaactagcta ttgatagtaa
     2041 tagttatgcc cacaatctat cctatcttgt cttgaacaag gaatacttga aagggcatgg
     2101 tatcggaagt agttatatca tggagttgta taccgactat ggtatgattg gagtctttct
     2161 gcttagtttc ttactcggcg tattatttat agccatgctg caagtagcct atcgctcaag
     2221 gacaatccta tttgctttat ccctactcat cttgaataat ctattcttta tgccaagaag
     2281 cagcttttca gaaagtttct tcaatttatt tacaatgcaa ttctggggaa ttgttcttgt
     2341 gattatattt gtagcaaaaa tgcttacaaa ggaaaactag tatctactaa acaaaggaga
     2401 aaaaaatcat gtttgaacat tattcagtag ctgatttgtt tgcaaatctt tataaaaagc
     2461 gaaaagcaaa tattttagct ctcatcgctt tatttgctct cattgctgta ccatttacaa
     2521 ttaaagcagt taggaataaa aacactgtca aagatacaac aagttattca acttatctta
     2581 tctataaaat cactcctcca gaagattcgg ccaaaacgat tttgaatcat caaattggtg
     2641 gttatagtga tttttatggg aaattgattg atggtaattt gaatggagct tatcttttca
     2701 atgatgtaga acccagtgag ttgaaaaaaa ttgccagtga attagacacg acagaaacaa
     2761 ccttgaaaaa ttcaacgaat gactattggt ggaaaaaatt gaccgtctac tatatgattg
     2821 acgatgcagg ggttggtgtg aaaattttga catcaagtaa agatgccaat gacttgttag
     2881 agaaaaaaat tgatgggttg attgagaaat ttaaacatgc ttatgcaaat gtgaaaattg
     2941 aaaaactgga aaccatcaac tctaaagaat tgaacgcaaa tggtgaaaca gcgcttggct
     3001 taaatgtgaa aaatctgatt cttcgtttag ttgttattgg agtggtttgt gtgattttgg
     3061 ttgtgatggg aaatgtgtta gtttatctct ttaatccaac aatcaataga gtaggtgatt
     3121 tttctcagta tcaaattgat tttgtaacag agattacaac aattgctaac ctagcagatg
     3181 ttttgtcata caaaaatact ggacaggaat tgaccatcgt tagctcaaat aaagctatcc
     3241 tagataaatt gaaacagagt caagaagctt taaaaggaat gcattttgta gatttacagg
     3301 atgtatcatc tcttttggaa agagatacag tccttcttgt tgaagagtac ggagtgactc
     3361 gttataagaa atttgagcaa agtcttcaaa ttctcagaaa cttaaatcgt tctatcctcg
     3421 gtgtagcaac ctttaaatta taagtattct gatgtatagg gtcttcaaag tcggctaaga
     3481 gccgattttg aaggcttttt ctatctgtct gcagaaattt tctcttggag tctcctatag
     3541 aaagacccta gggaaaatct atccgtatag aggagccttc ttttgataaa atcgtaaaaa
     3601 tctagtataa tagatagaac tgaaagtatg aggttactag caatgaaaat gaaacaaatt
     3661 agtgatacaa ctttaaaaat cacgatgtct ttagaggatt tgatggatcg tggaatggag
     3721 attgctgact ttctcgttcc tcaagaaaaa acagaagagt tcttttatgc tatcttggat
     3781 gagctagaga tgcctgatag ctttctggat acaggcatgt tgagcttccg tgtgactcca
     3841 aaacctgata aggtagatgt ctttgtgacc aagtcaaaga ttgaccaaaa tctagatttt
     3901 gaagacttat cggatttgcc agatatggaa gaattggctc aaatgtctcc agatgaattt
     3961 atcaaaaccc tggaaaaaag catcgcagac aaaaccaagg atgatatcga agccattcaa
     4021 tctcttgagc aagttgaagc caaggaagaa gagcaagagc aggctgaaca agaagctgag
     4081 agtaagaaag aaccttacat ctactacatc ctttcttttg ctaagttggc tgacttggta
     4141 gcttttgcca agacagtgac ttttgaaatg gaaacttctg aactctacaa aatgaacgag
     4201 cgctattatt tgaccatttt agtggatatt gaaaatcatc caagcccata tccagcttgg
     4261 ctgttggccc gtatgcgcga gtttgcagac gatagtgata tcagtcgctc agtcttacaa
     4321 gagtatggtc aagtcttgat gagtcacgat gcagtgctca atctgcaaaa aatcggctaa
     4381 tcctttctgg aaagctattt tcagatttta agaagagcga atcgtctgat tcgttttttc
     4441 tttttatact gaaatagtga tttactataa taggaatttt cacaaaattc tgttataatg
     4501 gctatattag aaaatttcga ggagataaag atgacagtta aaattgcttt actaggattt
     4561 ggtaccgttg caagtggtgt gcctttcctc ctaaaggaaa atggaggaaa aatcaatcaa
     4621 tcagcacatt cagatatcaa agttgctaag gtattggtca aggatgaaga tgaaaaaaat
     4681 cgcttgcttg cagcagggaa tgactttaac tttgtaacca atgtggatga tattttatca
     4741 gaccatgata ttactatcgt agtggaattg atggggcgta ttgagcctgc taaaaccttt
     4801 atcactcgtg ccttggaagc tggaaaacac gttgttactg ctaacaagga ccttttagct
     4861 gtccatggcg cagaattgct agaaatcgct caagctaaca aggtagcact ttactacgaa
     4921 gcagcagttg ctggtgggat tccaattctt cgtactttag caaattcctt ggcttctgat
     4981 aaaattacgc gcgtgcttgg agtagtcaac ggaacttcca acttcatggt gaccaagatg
     5041 gtggaagaag gctggtctta cgatgatgct cttgcggaag cacaacgtct aggatttgca
     5101 gaaagcgatc cgacgaatga cgtagatggg attgatgcag cctacaagat ggttattttg
     5161 agtcaatttg cctttggcat gaagattgcc tttgatgatg tagcccacaa gggaatccgc
     5221 aatatcacac cagaagacgt agctgtagct caagagcttg gttacgtagt gaaattggtt
     5281 ggttctattg aggaaacttc ttcaggtatt gctgcagaag tgactccaac cttcctacct
     5341 aaagcgcacc cacttgctag tgtgaatggc gtaatgaacg ctgtctttgt agaatctatc
     5401 ggtattggtg agtctatgta ctacggacca ggtgcgggtc aaaaaccaac tgcaacaagt
     5461 gttgtagctg atattgtccg tatcgttcgt cgtttgaatg atggtactat tggcaaagac
     5521 ttcaacgaat atagccgtga cttggtcttg gcaaatcctg aagatgtcaa agcaaactac
     5581 tatttctcaa tcttggctct agactcaaaa ggtcaggtct tgaagttggc tgaaatcttc
     5641 aatgctcaag atatttcctt taagcaaatc cttcaagatg gcaaagaggg tgacaaggcg
     5701 cgtgtcgtta tcatcacaca caagattaat aaagcccagc ttgaaaatgt ctcagctgaa
     5761 ttgaagaagg tttcagaatt cgacctcttg aataccttca aggtgctagg agaataagat
     5821 gaagattatt gtacctgcaa ccagtgccaa tatcgggcca ggttttgact cggtcggtgt
     5881 agctgtaacc aagtatcttc aaattgaggt ctgcgaagaa cgagatgagt ggctgattga
     5941 acaccagatt ggcaaatgga ttccacatga tgagcgtaat ctcttgctca aaatcgcttt
     6001 gcaaattgta ccagacttgc aaccaagacg cttgaaaatg accagtgatg tccctttggc
     6061 gcgcggtttg ggttcttcca gctcggttat cgttgctggg attgaactag ccaaccaact
     6121 gggtcaactc aacttatcag accatgaaaa attgcagtta gcgaccaaga ttgaagggca
     6181 tcctgacaat gtggctccag ccatttatgg taatctcgtt attgcaagtt ctgttgaagg
     6241 gcaagtctct gctatcgtag cagactttcc agagtgtgat tttctagctt acattccaaa
     6301 ctatgaatta cgtactcgcg acagccgtag tgtcttgcct aaaaaattgt cttataagga
     6361 agctgttgct gcaagttcta tcgccaatgt agcggttgct gccttgttgg caggagacat
     6421 ggtgaccgct gggcaagcaa tcgagggaga cctcttccat gagcgctatc gtcaggactt
     6481 ggtaagagaa tttgcgatga ttaagcaagt gaccaaagaa aatggggcct atgcaaccta
     6541 cctttctggt gctgggccga cagttatggt tctggcttct catgacaaga tgccaataat
     6601 taaggcagaa ttggaaaagc aacctttcaa aggaaaactg catgacttga gagttgatac
     6661 ccaaggtgtc cgtgtagaag caaaataaag aatagaatat aggatgggga aactcttgac
     6721 cagaggggtt catatccttt ttgtgaaaag aag
//
LOCUS       DAVAAL010000098         6659 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVAAL010000098 DAVAAL010000000
VERSION     DAVAAL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.099"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..980)
                     /gene="phtA"
                     /locus_tag="VQM60_001579"
     CDS             complement(<1..980)
                     /gene="phtA"
                     /locus_tag="VQM60_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET1081434.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRENLSNLRTYRRQNSDNTPRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLIFDPAQITSRTARGVAVP"
     gene            complement(1233..2966)
                     /gene="ptsP"
                     /locus_tag="VQM60_001580"
     CDS             complement(1233..2966)
                     /gene="ptsP"
                     /locus_tag="VQM60_001580"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET1081435.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQRAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(2972..3235)
                     /locus_tag="VQM60_001581"
     CDS             complement(2972..3235)
                     /locus_tag="VQM60_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET1081436.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            3586..3804
                     /gene="nrdH"
                     /locus_tag="VQM60_001582"
     CDS             3586..3804
                     /gene="nrdH"
                     /locus_tag="VQM60_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET1081437.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            3885..6044
                     /gene="nrdE"
                     /locus_tag="VQM60_001583"
     CDS             3885..6044
                     /gene="nrdE"
                     /locus_tag="VQM60_001583"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET1081438.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            6232..>6659
                     /locus_tag="VQM60_001584"
     CDS             6232..>6659
                     /locus_tag="VQM60_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HET1081439.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYL"
BASE COUNT         1909 a         1420 c         1257 g         2073 t
ORIGIN      
        1 gggacagcta cacctctggc ggttcgactt gtgatttgcg ctgggtcgaa aataaggcca
       61 tcagattcca catggcgttg actcaaaggc agtttgtaga gctgtttcaa gagactatca
      121 atgtcattac tttgacttgc ttgactgtta gtgttgctgt tgttgcttgt gttagtattt
      181 gtagttcctg gattgcttac agaaggtacc cagtttgttc ttggagtgtt atcgctattt
      241 tgtcggcgat aggttcttaa atttgacaga ttttcccgac cagataggaa ggcttctgca
      301 gcagccaact cgctagctga taactcattc ttaggaatgt aatggtaatg atctccatga
      361 ggaacgatat aagcatcacc agtgtcctca atgatatcag atgcattgaa gatataccca
      421 tcatctgtgg tgtagcgtcc ctgtgaacgt gcaaaggcta ccgcaccatc gtttgttgaa
      481 gtccctcctt cacgatgctg actatgttct tgtttttgtc gattgatttc ctcttttgta
      541 cggacgttat ccgcgtgggc agcatcctta aggtaaacat agtattttcc atctaccttg
      601 ataacatatc cacccttgac ctcattaaca atatcctcat cttttagctt atagtttgga
      661 tctttcatga gtaattcttc actgaaaatc gcatcataag gaaccttccc attgtaataa
      721 tgatagtggt cgccatgtga agtgacatag ccttggtctg ttatcttgat gacgatttgc
      781 tcagcattga ttccttcacg cttgctaacc tcatcaggag tcaagttctc cgttttttgc
      841 gtcgcttgtt ttccatctat ataggaaaca cgattatttt ccttaaccgt tctagcttga
      901 tacagtccca actcgtaaga acaaacactt aaaatcaaag ctgccgcaga accaacaagg
      961 tatttcttat taattttcat cgaaactctc tttctatttg atatttctag ttgcaattca
     1021 tttctaaatt gcagaaactc ctctctatta cttaactggt taatagttta ctcctaaatc
     1081 tccaacttgt caagaatttt atgaaccatt taagtatttt ttgttctgac cactactttt
     1141 tcaaggttgg actttctata agaaagattt tcttgaaaat ttttttaaaa tatcaaaaaa
     1201 atccctgtaa ctgagttgca gggacttttc gattaatcaa aattaacgta ttctttttga
     1261 agttcaagaa cttcttccat tgttgagcat tctgtaaggg cacggtttgc gtactcttcc
     1321 atcttagctg tgtcgagttt cttcatcaag ctgcgtgtac gaagtacaga tgttgctgac
     1381 atagagaact catccaagcc cattccgaca agaagtggaa cagctcgttg gtcaccagcc
     1441 atctcaccac acataccagc ccatttacct tcagcgtgag ctgctttgat cacattgtta
     1501 atcaagcgta ggattgatgg gttgtatggt tggtaaaggt atgaaacttg ttcgttcata
     1561 cggtctgctg ccattgtata ttggatcaag tcgtttgtac caattgagaa gaagtcaact
     1621 tctttagcaa attggtctgc aagcatagcc gctgcaggaa tctcgatcat gataccaact
     1681 tggatgttat ccgcaactgc aacaccttca gcaagaaggt ttgctttttc ttcatcaaag
     1741 actgctttcg ctgcacggaa ttctttcaag agcgcaacca ttgggaacat gatacgcaat
     1801 tgaccgtgaa cagacgcacg aagaagagca cggatttgtg tgcggaacat agcatctcca
     1861 gtctcagaga tagagatacg aagagcacgg aatccaagga atgggttcat ttcgtgaggc
     1921 atatcgaagt aaggaagttc cttatctcca ccgatatcca ttgtacgaac gacaacaggt
     1981 ttaccgttca ttccttcaag aacagccttg tatgcttcat actgctcatc ttcagttggg
     2041 aagtcttgag aatccatgta caagaactct gtacggtaaa gtccaacagc ttctgcaccg
     2101 ttgttgttaa caccttcaac gtcttttgga gtaccaatat tggcagccaa ctcgaagtgt
     2161 ttaccgtcag cagtcactgt ttgagcatct ttcaaaagtg cccattcagc tttttgtttc
     2221 gcataggctt caccagctgc tttaaattct gccgcttgtt catctgttgg gttgataatc
     2281 acttctccag tgatcccgtt aacagcaagg atgtcaccgt ctttaacgat ttcagtgatg
     2341 ttgtttgtac ctaatacagc agcaatttca agtgtacgtg ccatgatagc tgagtggctt
     2401 gtacgtccac cgatgttggt tacaaaagct tttacaaagt ttttgtccaa ttgagctgta
     2461 tctgaaggag tcaagtcatg cgcaatcaca atcacttctt cattgataga agctgggttt
     2521 ggcaattttt taccaagaag gtttgccaat acacgttttg tcacgtcgcg gatatccgct
     2581 gcgcgttctt gcatgtatgg gttgtcttcc atgccttcaa agatagtgat aaacatatct
     2641 gtaacttctt tcagacctgc ttctgcattc actttcttcg cacggatagt ttccttgatt
     2701 tggctgatca tttctgggtc agcaagaacc attaagtgag catcaaaaac ttgagctgct
     2761 tcttcaccga gcgtacctac tgctttctcg cgaataacag aaagctcgtc ttgtgatgcc
     2821 tgtagagcgg catcaaggcg agcttcttct gcgtttgtat cttcgactgt aatagtctca
     2881 aatgacaaat ccggctgaac gagtagatat gcttttgcaa ctgcaacacc gtcagatgct
     2941 gcgattcctt taagcatttc tgtcatttcc cttatgccaa tccttctttt tccattgttt
     3001 ctgagattgc agcgatagcg tcatctgcat ctgcaccttc agctgagata gttacgtcag
     3061 caccttggcc aacaccaaga ctcataacac ccataattga tttaaggtta actgatttac
     3121 ctttgtactc aagagtgata tctgaagcaa atttgctagc agtttgtacc aacaatgttg
     3181 ctggacgtgc gtgaatacct gtttctgcca ctacgtggaa atctttagaa gccatagttt
     3241 gactctcctt ttgttctttc tttttttgag ttatatgtga taacccttac aatttagtat
     3301 tataccatct tctaggattt ttttcaagca ttttctggat tttccttcaa tttcctttca
     3361 gataacttgc tgaaaatctt ctatcctaga taacaaaaca caggatatag tttctttaaa
     3421 aacaatttat aggataatca ttgctatttc acaatacaaa cactatatat tgtgtttata
     3481 tataaaaaca acaaaagaac accactatat ttagttcaaa tcattgacaa aattttattt
     3541 tctgatatac taagatagta ttaaattttg aagaggagtt acacaatggt aaccgtttat
     3601 tctaaaaaca attgtgtcca atgtaaaatg accaagcgtt tcttggacag taataatgtc
     3661 tcttatcgtg aaatcaatct tgatgagcaa cctgagtacg tcgatcaagt taaagagctc
     3721 ggttttagcg cagctcctgt tatccaaaca ccaactgaag tcttttcagg tttccaacca
     3781 ggaaaactga aacaattagc ataatcttag tacatcatcc agaagaaact gcttttaggg
     3841 ctaacttaga agcctttctt ttgtaattag ataagggaaa ttttatggga ttaaaacatc
     3901 ttgaagacgt gacttacttc cgtcttaata acgaaattaa ccgtcctgtt aatggacaaa
     3961 tcatgcttca taaagacaag gaagccttgg atgctttctt taaagaaaat gtagttccaa
     4021 acactatggt ttttgattca atcaaagaca aaatcaatta cctcattgaa cacaactaca
     4081 tcgaaacagc ctttatcaag aaataccgtc cagagttctt ggaagaattg gctcaattta
     4141 tcaaagacca aaacttccaa ttcaagtcat tcatggcagc ttataaattt tacaatcaat
     4201 atgccttaaa gactaatgac ggtgaatact atcttgaaaa tatggaagac cgtgtcttct
     4261 tcaacgccct ttatttcgct gatgggaatg aagctgttgc aatcgatatt gccaatgaaa
     4321 tcatccacca acgctaccaa cctgctactc cttccttctt gaatgctgga cgtgctcgtc
     4381 gtggggagtt ggtatcttgt ttcctaatcc aagtgacgga tgatatgaac tctatcggac
     4441 gttctatcaa ctcagctctt caactttcac gtattggtgg tggtgtggga attaccctca
     4501 gcaaccttcg tgaagctggt gcacctatca aaggctatga aggagctgct tcaggtgtcg
     4561 ttcctgttat gaagcttttt gaagacagct tctcttactc caaccaattg gggcaacgtc
     4621 aaggtgccgg tgttgtctac ctcaacgtct ttcacccaga tatcatcgct ttcctttcaa
     4681 ctaagaaaga aaacgctgat gaaaaagtac gtgtcaagac tctatcactt ggtgttgtag
     4741 tacctgataa attctacgaa ttggctcgta aaaatgaaga aatgtacctc ttcagccctt
     4801 actctgtaga aaaagaatac ggtgtgccat tcaattacat cgacattact gaaaaatacg
     4861 atgaattggt cgcaaatcca aatattcgca agacaaaaat caaggcgcgg gatttggaaa
     4921 ctgaaatctc taaattgcaa caagagtctg gctatcctta tgtagtcaac atcgatacag
     4981 ctaaccgtgc aaatcctgtt gatggaaaga ttatcatgag taacttgtgt tctgagattc
     5041 ttcaagttca agaaccaagc ttgatcaacg atgctcaaga attccttcaa atgggaacag
     5101 atgtttcatg taatctgggt tcaaccaacg tggttaacat gatgacttca cctgattttg
     5161 gtcgttctat ccgtgctatg gttcgtgccc ttactttcgt tacagatagt tcacatatcg
     5221 tagctgtacc aacgattgac catggaaata gccaagccca cacctttggt ctcggtgcca
     5281 tgggactgca cagctacctt gcccaacaac tcattgaata tggttcacct gagtctgttg
     5341 aatttacaag catctacttt atgcttatga actactggac cttggtagag tcaaacaata
     5401 ttgcgcgtga acgtggtatt accttccaca actttgaaaa atcagactat gctaacggaa
     5461 gttactttga caagtatgta actggtgaat ttgttccaac atcagaccgt gttaaagaac
     5521 tcttcaaaaa tgtttttatc cctggtgttg ctgattgggc tgaacttcgc gacaaggttc
     5581 aagaagatgg tctttaccac caaaatcgcc ttgctgtagc gccaaatggt tctatcagct
     5641 atatcaacga cgtttctgct tctatccacc cgattacgca acgtatcgaa gaacgccaag
     5701 agaagaaaat tggtaaaatc tactaccctg ctgctggctt gtctacagaa accattcctt
     5761 actacacttc tgcctacgat atggatatgc gtaaagtcat cgatgtttac gctgctgcga
     5821 ctgaacacgt ggatcaagga ctttcactca ccctcttcat gcgcagtgac attccaaaag
     5881 gcctttacga atggaagagg gaaaataaac aaacgacacg tgacttgtcc atccttcgta
     5941 actatgcctt taacaaggga atcaagtcta tctactacgt ccgtaccttt acagatgacg
     6001 gtggagaagt cggcgccaac caatgtgaaa gctgtgtgat ttagtttcta gcatagaaac
     6061 actatttttc cctaagcagc actgataaaa tcaaatctac tatgtcataa cataatttat
     6121 gtaacatttt aaaagtcggg cttccgactt tttgtgcgat actcctatag aattatggta
     6181 aagtagaaat aattgtgagt tcgcaatact aaacacagaa agagatttta aatggaaact
     6241 tactacaaag ccattaactg gaatgccatc gaagatgtca tcgacaaatc aacttgggaa
     6301 aaactgacgg agcaattttg gctcgataca cgtattccct tatcaaacga cttggatgac
     6361 tggagaaagc tatctaacaa agagaaagac ttggtcggaa aagtttttgg tggtttaacc
     6421 cttcttgaca ctatgcaatc tgaaactggg gttcaagccc ttcgcgcaga catccgtaca
     6481 ccacatgagg aagctgtttt caataacatc caatttatgg aatctgtcca cgctaaatct
     6541 tactcatcaa tcttttctac cttgaatact aaggctgaga ttgaagaaat tttcgaatgg
     6601 accaatacca atccttacct acaaaagaag gctgagattg tcaacgaaat ctacctaaa
//
LOCUS       DAVAAL010000099         6562 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVAAL010000099 DAVAAL010000000
VERSION     DAVAAL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.100"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(200..439)
                     /locus_tag="VQM60_001585"
     CDS             complement(200..439)
                     /locus_tag="VQM60_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET1081440.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(459..731)
                     /gene="rpsP"
                     /locus_tag="VQM60_001586"
     CDS             complement(459..731)
                     /gene="rpsP"
                     /locus_tag="VQM60_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET1081441.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            901..1110
                     /locus_tag="VQM60_001587"
     CDS             901..1110
                     /locus_tag="VQM60_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081442.1"
                     /translation="MLSVNTILEKFYKKHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            1589..1801
                     /locus_tag="VQM60_001588"
     CDS             1589..1801
                     /locus_tag="VQM60_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081443.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            1885..2688
                     /locus_tag="VQM60_001589"
     CDS             1885..2688
                     /locus_tag="VQM60_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET1081444.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(2744..4285)
                     /locus_tag="VQM60_001590"
     CDS             complement(2744..4285)
                     /locus_tag="VQM60_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET1081445.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(4287..4769)
                     /locus_tag="VQM60_001591"
     CDS             complement(4287..4769)
                     /locus_tag="VQM60_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET1081446.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            complement(4769..5671)
                     /locus_tag="VQM60_001592"
     CDS             complement(4769..5671)
                     /locus_tag="VQM60_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HET1081447.1"
                     /translation="MKPSIHSLAHQTMQKWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDID
                     AACGQLRFNTMKRDRQKAVAAVNP"
     gene            complement(5692..6222)
                     /locus_tag="VQM60_001593"
     CDS             complement(5692..6222)
                     /locus_tag="VQM60_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET1081448.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(6379..>6562)
                     /locus_tag="VQM60_001594"
     CDS             complement(6379..>6562)
                     /locus_tag="VQM60_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET1081449.1"
                     /translation="VTSTANQDTPISEGKKPILGLDVWEHAYYVKYRNVRPDYIKAFF
                     SVINWNKVDELYAAAK"
BASE COUNT         1962 a         1399 c         1208 g         1993 t
ORIGIN      
        1 ttaatgttcg attaagtagt cttggaattg cctaaacgtt caatctttta tagtatattg
       61 aaactagaat agtacacctt tacttctaaa acattgttag aaatcgattt gactgtcctg
      121 atcgatttgt cctgttcttg tttcatttta ctatatcttc tattccacac aaaaaagcga
      181 gacatccgtc ccgcccttct tatttttcgt caataacgat tcttactttt ttgtattcag
      241 ttgggacaga gtagacaatc gttcttatcg cagaaatagt gcgaccctta cgaccgatta
      301 cacgacccac atcgctttga tcaagattca aatgatattc caaaaattct ggtgtatcct
      361 caatcttgat agttaaggca tctggttgtg aaattaaggg tttcacaatc gcaataatga
      421 gattttcaat cgtatccatc tgtcaaccta ctttaaactt attttgaaaa tttagaatcg
      481 tggaattttt tcaatacgcc ttcttttgaa aggatgttac gtactgtgtc tgaaggttga
      541 gctccattag ccaaccatgc aagaacgcga tcttctttca aagttacttg gttttcagca
      601 acaagtgggt tgtaagttcc aactgtttcg atgaaacgtc cgtcacgtgg tgaacgtgaa
      661 tctgctacgt tgatacggta gaaaggtttt ttcttagaac ccatacgagt caaacggatt
      721 ttaactgcca tttttaaagt ctcatttctt taatttttta tttcggtgaa atagctgagc
      781 tatttagcac atgttctatt atagcagatt tctggcatgt gtcaagaaaa aaacttgaca
      841 gactataaat ttcttaaagt atttagaaat ttgttagaat aggaaataaa tgtttgaaag
      901 atgctatccg tgaatactat tttagaaaaa ttttataaaa aacaccaagt caaacccttc
      961 atatcgcctg aacgagagtt ggatacttgg cttctaagtc ccaagcccgt tcccaagcga
     1021 aatatggact tattagtaga tgattcacta gcaggagata tcattttact atggagaatc
     1081 cagtttggta cttttaccac tgaaacatga ttttaaaaac tcccattcga atatgattca
     1141 aaattcgctt agaatcaatg ttttccgact ttttcaaaaa ataaattttc atctaattta
     1201 aataactttc aattaagtgt gaattttgct taaaatcatt aaaaaacacc ccatggtgtg
     1261 aaccatgaag tgttgtaaat gctgtgttgt agctagttct tagcgtttag agaactggct
     1321 agctttacga gctttcttaa gatctggttt ccaaaatact ttgagtacac ttatggtatt
     1381 gatgccccta aacatctaaa aactctggta gaaaaagggt atgctcttgt cgaaacttct
     1441 tttgactcac ttgaccatct aaataatacc atgaaaaaga atatcctgaa gaagaaggga
     1501 gttactggac tttctaaaat gaaatcttgc gacctggatc aagcccttca tgagcatttt
     1561 tcagaagaag aattagctgg tcactttcat gtccttctat ggactttttt acaatggcat
     1621 tgctatcaca cccaatacct atctaagcgc ctggttcgta aactttattg cagctcttcc
     1681 tctaaatttc ctaatcgttg aaccaattgc ccgttttata ctaagttctt ttcagaaacc
     1741 atttactggg gaagaagttg aagattttca agatgatgat gaaatcccaa ctattatcta
     1801 agccagttct gtaaactact aatatttgaa atccacttcc ttttagggta caatggttat
     1861 aaatgaattt ttgagaggat cagaatgaaa aaactagcaa cccttctttt actgtctact
     1921 gtagccctag ctgggtgtag cagcgtccaa cgcagtctgc gtggtgatga ttatgttgat
     1981 tccagtcttg ctgctgaaga aagttccaaa gtagctgccc aatctgccaa ggagttaaac
     2041 gatgcattaa caaacgaaaa cgccaatttc ccacaactat ctaaggaagt tgctgaagat
     2101 gaagccgaag tgattctcca cacaagccaa ggtgatattc gcattaaact cttccctaaa
     2161 ctcgctcctc tagcggttga aaatttcctc actcatgcca aagaaggcta ctataacggt
     2221 attaccttcc accgtgtcat cgatggcttt atggtccaaa ctggagatcc aaaaggggac
     2281 ggtacaggtg gtcagtccat ctggcatgac aaggataaga ctaaagacaa aggaactggt
     2341 ttcaagaacg agattactcc ttatttgtat aacatccgtg gtgctcttgc tatggctaat
     2401 actggtcaac caaacaccaa tggcagccag ttcttcatca accaaagctc tacagatacc
     2461 tcttctaaac tccctacaag caagtatcca cagaaaatta tcgaagccta caaagaaggt
     2521 ggaaacccta gtctagatgg caaacaccca gtctttggtc aagtgattga cggtatggat
     2581 gttgtggata agattgctaa ggccgaaaaa gatgaaaaag acaagccaac tactgctatc
     2641 acaatcgaca gcatcgaagt ggtgaaagac tacgatttta aatcttaaaa accaaaaaaa
     2701 tacagtatcc acattcggta ctgtatttct tttactctca ttcttaagtt aaattattaa
     2761 aatcccatat ttggtctatc cagccttcat aaaagtctgg ctcgtggcag accataagga
     2821 tagatcccct atattctttg agagcgcgtt tgagctcatc ctttgcatcc acatccaaat
     2881 ggttggtcgg ctcgtccagc actaaaacgt tgttttcacg attcatcaag agacagaaac
     2941 gaaccttggc ttgctctccc cctgataata cttgaatctg gctttcaata tgtttggttg
     3001 tcaaaccaca acgggcaagg gctgcacgga cttctgcttg attaagggca ggaaaggcat
     3061 tccagacagc ttcaagagga gtttggcgat taccgccttc tacttcctgc tcaaaataac
     3121 caagttctaa ataatctcca cgctccactt ccccagcgat tggcgagata atgcccaaga
     3181 gactcttcaa gagagttgtt tttccaatac cattagcacc aataatcgca accttttgat
     3241 tgcgttcgaa ggtaagattt aaaggcttag taagaggacg gtcgtaacca atttgcaagt
     3301 tcttggcttg gaagataaag cgccctggtg tacgagctgg tttgaaatca aaggatggtt
     3361 ttggtttctc actttggagt tcgataatat ccatcttatc caatttcttt tgacgagaca
     3421 tagccatatt acgagttgca acacgggctt tattacgagc cacaaagtcc ttgaggtctg
     3481 caatctcttt ctgctggcgt tcgtaggctg cctctagctg agatttcttc atagcataaa
     3541 cttcttggaa ctggtagtag tcaccagagt aacgcgtcag ctgttgattt tccacatgat
     3601 agacaatatt aataacgtca ttgaggaatg gaatatcgtg cgaaatgaga acaaaggcat
     3661 tctcatagtt ttggagatag cgcttgagcc aatcaatatg ctcagcatcc aagtagttgg
     3721 tcggctcgtc caacagcaag atatcaggct tttcaaggag aagttttgcc aaaagcacct
     3781 tggttctttg cccacctgac aaagaagtta catccgtatc catgccaaag tccataacac
     3841 caagagcacg cgctacttcg tcaatcttag catccaaggt atagaaatca cgactctcca
     3901 gacggtcttg aagttctcct acttcttcca tgagagcatc aacatccgcg ccgtcttcag
     3961 ccattttcat atagaggtca ttgatacgag cttcagcttt gaaaagctca tcaaaagccg
     4021 tacggagaac atcacgcacc gactgtcttt cagcaaggac agagtgctga tccaagtaac
     4081 cagccgtcac atatttggac cactcaacct ttccttcatc tggcagcatt ttaccagtca
     4141 cgatactcat aaaggttgat tttccttcac cattggcacc gaccaggccg atatgttctc
     4201 ccttgaggag acggaaggac acatcttcaa aaattgcacg gtcaccaaaa ccgtgactca
     4261 gatttttaac ttctaaaata ctcattttaa ttccttacct tgtttttatg taatcgttta
     4321 taaaggagcc aagccagata gccacccaaa gtgttggtcc acaaatcatc aatctcaaag
     4381 acgcgattga aatcaaagaa aaagtccaag attaattgcg tacactcgat tccaagactc
     4441 acaagaaaac taaaaagaag gacctttttt gttttccgca aatttggaaa tagataaagg
     4501 agttggaaaa tcagaggaaa aaacaagaag acattgagga tattttgtaa aaaaatccaa
     4561 cataattgtc caatgtcact cacttcgccc agtttccaga gagaattgaa aggagtcaaa
     4621 agaaaaacca ggcgtccaag atgctgaata cctggagttc ccactcccac ggtagattgt
     4681 tcttgaggag taaagcaaaa acagacaatg caaatgctat agaaaatgac tccccagacc
     4741 aaaatatgat tataagtctt cttcatcatt aaggatttac cgctgcgact gccttctggc
     4801 ggtcacgttt cattgtgtta aagcgcaatt gtccacaagc tgcgtcaata tctgtaccat
     4861 gctcttgacg aaccacacag ttgccccctt ttttcttaag cgtatcatag aaagccaaca
     4921 cgcgctcttt gggactacgg ctatattggt catgctcact aactgggtta taaggaatca
     4981 agtttacata agacaatttc ttgatgttct tgagcaattc agccaattcc aaggcttgtt
     5041 ctacaccgtc gttgacttca ttaagcatga tatattcaaa ggttacacga cggtttgttg
     5101 tctcaatgta gtattcaata gcagcaaaga gtttttcaat cggaaaggca cggttaatct
     5161 tcatgatact tgaacgaagt tcattgttgg gtgcgtgaag agacacggca agattgacct
     5221 gaaccccttc atcagcaaag tatttctgaa ccagcatgat ttgcgctacg atttcaccgt
     5281 tattgaggtc acgctgcttc ttaatcaaac cagatgcaca gaaggtacaa ccgatattac
     5341 agccgacctg agtggtcaca cagacagata aaccatagtg ttgacgcatg agtacagtct
     5401 caattaacat accgtcaggc aattcaaaga gatatttgac tgtaccatca gcagactctt
     5461 gcacaatacg ttgtttcaag ggattgacca caaactggtc attgagctta gcaatcaaat
     5521 ccttggaaag gttggtcatt tcttcaaatg actgcacacg tttacggtag agccattccc
     5581 agatttgatc tgcacggaat ttcttttctc cctgctccaa tacccatttc tgcatggttt
     5641 gatgtgccaa actatgaatt gagggtttca tttcttctcc ttattctcta ctcacttctg
     5701 acgaatgaca aaatgacgtt gtcccttgtc ctctttctga gaatgctggt ctttctgacg
     5761 acgtctattt ttcttatctg cattcgactt tcgtttagtt tgagtcggtt tctttccttt
     5821 tctagaaggt gtttcttctt ccgtcttacg cattttcttg tcaaatgatg ctcgcttagg
     5881 ggcttcattt tctaagacaa aataggcaca accataacta caatactcta aaaggtagtc
     5941 ttgtaaacga ctgatttttt caagtttttc ttctgttcgg tcatccttgt aaaaacctcg
     6001 taggcgaagc tgttcgttgc tccagtcccc cacgatataa tcaaacttgg ttaagacttc
     6061 tgaaaaacgc tgattaaaag tcgtcacatc aaaggcatcc ttgatatttt caaccaagga
     6121 aaaagctatc ccttccgttt cgaccttgtc cccgtgtaaa tggaactccg gaccaggaaa
     6181 cttgttatag ttgtataatt caggtgcaat ttcttttcgc atagatatcc tttttccacg
     6241 attacttaat actttattct accataattt ctagcagtta gcacgtttct cataaaaatg
     6301 aaaaaagtct gacgattttg tcataccaga atcttataac ctaaaaaaga gaagaacaat
     6361 tcttccctcc aactattatt atttagcagc tgcgtacaat tcatctactt tattccagtt
     6421 gattactgaa aagaaagctt tgatgtagtc aggacgcacg ttgcggtatt tcacgtagta
     6481 agcatgttcc caaacgtcca agcccaagat tggtttttta ccttctgaga ttggtgtgtc
     6541 ttggtttgct gttgaagtca ct
//
LOCUS       DAVAAL010000100         6532 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVAAL010000100 DAVAAL010000000
VERSION     DAVAAL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.101"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            174..827
                     /locus_tag="VQM60_001595"
     CDS             174..827
                     /locus_tag="VQM60_001595"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET1081450.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            837..1301
                     /locus_tag="VQM60_001596"
     CDS             837..1301
                     /locus_tag="VQM60_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET1081451.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECATREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1314..2582
                     /locus_tag="VQM60_001597"
     CDS             1314..2582
                     /locus_tag="VQM60_001597"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET1081452.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFKAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2596..2856
                     /locus_tag="VQM60_001598"
     CDS             2596..2856
                     /locus_tag="VQM60_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081453.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2867..4228
                     /gene="pdrM"
                     /locus_tag="VQM60_001599"
     CDS             2867..4228
                     /gene="pdrM"
                     /locus_tag="VQM60_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET1081454.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAVFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4382..5350
                     /locus_tag="VQM60_001600"
     CDS             4382..5350
                     /locus_tag="VQM60_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET1081455.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5366..6358
                     /locus_tag="VQM60_001601"
     CDS             5366..6358
                     /locus_tag="VQM60_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET1081456.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
BASE COUNT         1868 a         1239 c         1512 g         1913 t
ORIGIN      
        1 aaatgcttaa tgatttaaaa agtaattata aaaaatagcc agtctttata aactgtattc
       61 acgtattcaa atcgtatata gtcgatttat taaatcttta gataaattgt aaaaagtggt
      121 ttccatagcc actttttgct ataatagagg cagtaaaaat gaaggagtca gctatggaac
      181 actcgtcttg gcatgctttg attaaggcgc aattacctga gggttatttc gggaaaatca
      241 atcagtttat ggagcaggtc tattctcagg ggattattta tccacccaag gaaaaggttt
      301 ttcaggctct cttgacaaca ctgcttgaag aagttaaggt ggtaattcta gggcaagacc
      361 cctatcacgg accaggtcaa gcgcagggct tgagtttttc tgtacctgac tctatcccag
      421 ctccaccatc cttgcaaaat atcttgaaag aattgtcaga tgatatcgga gttaagaaat
      481 ctcatgattt gacagcttgg gctgagcaag gagtcttgct tcttaatgct tgtttgactg
      541 ttcctgctgg acaggccaat ggtcatgctg gtcaaatctg ggagcctttt actgatgctg
      601 tgattcaggt ggtcaatcat ctagatagac cagtcgtttt tgtactctgg ggagcttatg
      661 cacgtaagaa gaaggcctta gttaccaatc ctcatcactt gattatcgaa tcagcccatc
      721 caagtccttt gtcggtttat agaggatttt ggggttccaa gcccttttcc aaggccaatg
      781 cattcttaaa agagacagga caagagccaa tcgattggct tagataagga gaaaatatgc
      841 ctcagttagc gacgatttgc tacattgata atgggaaaga actgctcatg ctccatcgta
      901 ataagaaacc caatgatgtc catgaaggga aatggattgg tgtgggtggt aagctagaga
      961 gaggagagac gccccaggaa tgcgcgacgc gtgaaatcct tgaagaaaca gggctcaaag
     1021 ccaagccagt tctaaaaggt gtcatcactt ttcctgaatt tacaccagat ttagactggt
     1081 acacctatgt ttttaaggtg acggagtttg agggcgactt gattgactgc aatgagggga
     1141 cgctagaatg ggttccctat gatgaggttt tgagcaagcc gacttgggaa ggtgaccaca
     1201 cctttgttga gtggctttta gaggataaac ccttcttttc agccaagttt gtttatgatg
     1261 gggataaatt gttggatacc caagttgatt tctatgaata aaggagaaag cagatgctac
     1321 taatcaaaaa tggtcgtgta atggatccca agtctggttt ggatcaagtt tgtgatgtct
     1381 tagttcaaga tgggaaaatt atcaaaattg cgcctgagat cacggaagaa ggagcagaaa
     1441 cgattgatgc tactggtctt gtggttgctc ctggtttggt cgatattcat gtgcatttcc
     1501 gtgaacctgg tcaaacacat aaagaagaca ttcatactgg tgccctagca gccgctgcag
     1561 gtggttttac tactgtcgtc atgatggcta ataccagtcc aaccatttca gacgtggaga
     1621 ctttgcaagc agttctccag tcagctgcca aagagaagat taatgtcaag acagttgcga
     1681 ccattactaa aaactttaat ggtaaaaact tgactgactt taaggcactc ttagaagctg
     1741 gtgcggttgg tttctctgat gacggtattc cgcttgagag cagtaagatt gtcaaggaag
     1801 ccatggagga agccaaaaaa ctcaatacct ttatcagcct tcatgaggaa gatccaggtt
     1861 tgaacggtgt tcttggcttt aatgaaaata ttgctagaga acatttccat atctgcggtg
     1921 ctactggggt ggctgagtac gctatgatgg cgcgtgatgt catgattgcc tatgcaacta
     1981 aagcccatgt tcacatccag catttgtcta aggaagaaag tgttaaagta gtggagtttg
     2041 ctcaggggtt aggtgcagaa gtcacagcag aagtagcgcc acagcatttc tctaagacag
     2101 aagcacttct tttaacacaa ggtagcaatg ctaagatgaa tccaccgctt cgtttggaat
     2161 cagaccgtcg tgccgttatc gaaggtctca aatcaggtgt catcacagtt attgcgactg
     2221 accacgcgcc tcatcatgta gatgaaaaaa atgttgagga tattaccaaa gcgccatctg
     2281 gtatgactgg cttagaaaca tccctgtctc tcggcttgac ctatttggta gaagctggtg
     2341 agttgagctt gatggaatta cttgaaaaaa tgacatacaa cccagccaag ctttacaact
     2401 ttaaagcagg ttacttggct gagaatggtc cagcagatat cactattttt gatgccaagg
     2461 ctgaccgcct tgtggactcc cattttgctt ccaaagcagc taattcacca ttcatcggtg
     2521 aaaccttaaa agggcaggtt aaatatacca tctgtaaggg acaaatcgtc tatcaagctt
     2581 gataggagtt agaatatgaa ttattttcga aaacgtaggg agagacaagc gaaatctaat
     2641 agtggaattt actgtcctgc agctaatcgt tccaaaattc aatatgaaac taaagaaaaa
     2701 gctgaccatg cggttcaata tgataacgga ggtttagtta gaagctatta ttgtcgtaca
     2761 tgtgcgtgtt ggcatactac ttcaaaatct aataaacctc cgctaagttt gaaaaactat
     2821 ggtctgaaac tctttggttt agataaatca gaatagaaag agaagtatgt ataagacaaa
     2881 gtgtttacga gagaagttgg tattattttt aaaaattttc ttcccaatcc tgatttacca
     2941 atttgccaat tattctgcct cttttgttga tactgcaatg acaggtcaat acaacactat
     3001 ggacttggct ggtgtatcta tggcaaccag tatctggaat cctttcttta catttctaac
     3061 agggattgtg tcagccttgg tgcctatcat tggtcaccat cttggtcgag gaaaaaagga
     3121 agaagttgcg tctgattttt accaatttat ttatttggcc ttgggcctat ctgtggtctt
     3181 gctggggatg gtacttttct tggcaccaac aatcttgaat catattgggt tagaagcagc
     3241 agtagcggca gtagcggttc gctatctttg gtttttatct atcgggatta tccccttgtt
     3301 gctctttagc gtcattcgtt ccttgctgga ttcgctgggc ttgaccaaac tgtccatgta
     3361 cctcatgctt ttgttactcc ctctcaatag cggatttaac tatctcttga tttacggtgc
     3421 ctttggtgtt ccagaactgg gaggggctgg tgctggttta ggaacatcct tggcctactg
     3481 ggtcttgctt gggatttctg ttctggtttt atttaaacag gagaagctca aagccttaca
     3541 ccttgagaaa cgaattccac ttaatatgga taaaattaag gaaggagttc gtttaggtct
     3601 gcctattggg ggaactgtct tcgcggaagt ggctgtcttt tcagtggttg gcttgattat
     3661 ggctaagttt tcgtccttga ttatagctag tcaccagtca gctatgaact tttcaagtct
     3721 tatgtacgcc tttcctatga gtatctcatc ggctatggct attgtcgttt cctatgaagt
     3781 gggagccaag cgatttgatg atgcgaaaac ctatattggt ctaggaagat ggactgccct
     3841 catttttgcg gccttcacct taaccttcct ttacattttt aggggaaatg tggccagtct
     3901 ttatggtaac gacccaaaat ttatcgattt gacagtgcgt tttttaactt atagtctttt
     3961 cttccagtta gcagatacct ttgcggcgcc gcttcaggga attttgcggg ggtataagga
     4021 tacagttatt cctttttacc ttggtttgct tggttattgg ggcgtagcaa tccctgtggc
     4081 tacgctattt gattccctaa cagattttgg agcttattct tactggatcg gcttgattat
     4141 tagtttgatt gtgagcgggg cgctctaccg ttggcgttta actgtgatta tgaagagatt
     4201 tgaatcttta gcaaaatcca aacgctaaaa agtttgtgaa aaaatattct tgacaagaaa
     4261 atcccttgtt ttcacggggt tttctttttt attttgtgaa atttctttta gaaaaacttt
     4321 gacagtattt gccaaaagcg gtaaaatagt aaggtaagaa gaaagttaga aaggcaagaa
     4381 gatgtcaact ttagataaaa atcttttgct agaaatgttc cgtaagatgg aagaaatccg
     4441 tcgcatggac ttaaaaattg cacaattagt aaagaaaggg aaagtgccag gaatgacgca
     4501 cttttctgtt ggtgaagagg cagctaacgt gggggctatg ttagctctca atccagatga
     4561 tctgattacc tcaaaccacc gtggacacgg gcaagctatt gctaaaggga ttgacctcaa
     4621 cggaatgatg gctgaaatcc ttggtaaata cactggaacc tgtaaaggga aaggtggatc
     4681 tatgcatatc gctgaccttg atgctgggaa ccttggtgcc aatggtatcg taggtggtgg
     4741 tatggggatc gctgtcggtg cagccctcag tcagcaaatg caaaataccg gtaaaatcgt
     4801 tgtctgcttc tttggagatg gtgcgaccaa tgaaggtgtt ttccacgaag cagtgaacat
     4861 ggcttctatc tggaacctgc cagtcatttt ctattgcatt aacaacggtt acggtatctc
     4921 tgcggatatc aagaaaatga ccaatataga acatatccat caacgtagcg ccgcttatgg
     4981 aattcctgga atgttcatcg aagacggtaa caatgtcatc gatgtctatg aaggatttca
     5041 gaaagctgta gaccatgttc gcagtggcaa tggtccagtc ttgattgaaa gtgtaactta
     5101 tcgctggctc ggtcactcat catctgaccc tggtaaatat cgtacgcgtg aagaagtgga
     5161 attgtggaaa caaaaagatc caatcgaaaa cctccgcaat taccttattg aaaataacat
     5221 tgcaagtgcc gaagaattgg aagaaatcca agcgcaagta aaggaagcag tagaagcttc
     5281 tgttaaattt gcagaggaaa gcccattccc accgcttgaa tcagcctttg aagatattta
     5341 cgcagactaa ggagaaagag ataaaatgga aacaaaaaca atgtccttcc gtgacaccat
     5401 tatccttgct atgtctgagg aaatgcgtcg cgatgaaaat gtgttcttga tgggagaaga
     5461 cgtcggtgtc ttcggaggag acttcggtac ttctgttgga atgcttgaag aatttggtcc
     5521 agaacgtgtc cgtgactgtc cgatttctga agctgccatc tctggagcag cagcaggagc
     5581 agccatgaca ggacttcgtc caatcgtcga tatgaccttc atggacttct ctgttattgc
     5641 catggacaat atcgtcaacc aagctgctaa aacacgttac atgtttggtg gtaaaggtca
     5701 ggttccaatg actgttcgtt gtgcagctgg taacggagtt ggctctgccg cccagcactc
     5761 acaatctcta gagtcttggt ttactcacat tccaggactt aaggttgtgg cacctggaac
     5821 acctgcggac atgaaaggcc tgcttaagtc ttctatccgt gataacaacc cagttattat
     5881 acttgagtac aagtcagaat ttaaccaaaa aggggaagtg ccagttgatc cagactacac
     5941 aattccactt ggggttggtg aaatcaaacg ccaaggaacg gatgtaacag ttgttactta
     6001 tggaaaaatg cttcgccgtg tggttcaagc tgctgaagaa ttagcagaag aaggaatttc
     6061 agttgaaatt gttgacccac gtacccttgt tccgcttgat aaggatatca tcataaactc
     6121 agtgaagaag actggtaagg ttgttctggt caacgatgcc cacaaaacaa gtggctatat
     6181 cggtgaaatt tcagctatta tttcagaatc agaagcattt gactatctag atgcaccaat
     6241 tcgccgttgt gcaggagaag atgtgccgat gccttatgca caaaacctag aaaatgcaat
     6301 gattccaaca gttgaaagta tcaaagatgc aatccgaaaa acttataaca aagaatagag
     6361 ttacataaga taagttatgt aaataaaata aagctgacga gaaactttgt atcttttagg
     6421 tgcagagctc ttcgtcatta atatcttaga ttagagcacg ggctaaaagc tcggaaaaaa
     6481 gataaatctc cttggcttca tcgaagccat cgtcgatttc ctatttttca gt
//
LOCUS       DAVAAL010000101         6527 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVAAL010000101 DAVAAL010000000
VERSION     DAVAAL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.102"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(89..1012)
                     /locus_tag="VQM60_001602"
     CDS             complement(89..1012)
                     /locus_tag="VQM60_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET1081457.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(1026..1955)
                     /locus_tag="VQM60_001603"
     CDS             complement(1026..1955)
                     /locus_tag="VQM60_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET1081458.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(2274..2495)
                     /locus_tag="VQM60_001604"
     CDS             complement(2274..2495)
                     /locus_tag="VQM60_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081459.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(2449..2709)
                     /locus_tag="VQM60_001605"
     CDS             complement(2449..2709)
                     /locus_tag="VQM60_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081460.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            complement(3135..3290)
                     /locus_tag="VQM60_001606"
                     /pseudo
     CDS             complement(3135..3290)
                     /locus_tag="VQM60_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3475..4086)
                     /gene="rpsD"
                     /locus_tag="VQM60_001607"
     CDS             complement(3475..4086)
                     /gene="rpsD"
                     /locus_tag="VQM60_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET1081461.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(4281..5333)
                     /locus_tag="VQM60_001608"
     CDS             complement(4281..5333)
                     /locus_tag="VQM60_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET1081462.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(5330..6028)
                     /locus_tag="VQM60_001609"
     CDS             complement(5330..6028)
                     /locus_tag="VQM60_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1081463.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(6195..>6527)
                     /locus_tag="VQM60_001610"
     CDS             complement(6195..>6527)
                     /locus_tag="VQM60_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET1081464.1"
                     /translation="INVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT         1962 a         1358 c         1255 g         1952 t
ORIGIN      
        1 gttgacgcgg tttgaatttg attttcgaag agtattaact tcacacaagg gaagttggga
       61 actgagaaat gttctttttc aataagcact attctttcac accaccgata gtcaaacctt
      121 ttacaaagta gcgttggaag aatggatata aaatcgcgat tggaagggtt gcaaccacaa
      181 ccatggccat acgacctgtt tctttcggta gagcaactcc cagttgacca gtcaagccga
      241 ccgctttggc aatgtagtcc atattttgtt ggatttgcat gagcaaatat tgcaatggat
      301 acaagttatc actcttgatg taaagaaggg cgttgaacca gtcattccag aaaccaagag
      361 ctgtcaaaag cgtgatggtt gcgatacctg gtagtgacaa tggcaaacag atttggaaga
      421 aaatccgggc ctcactggca ccatcgatac gagccgattc gagaatggct tctggaatgg
      481 tcttcttgaa gaaggaacgc atcaagatga tattgaatgg tgagagaagc attggaacaa
      541 tcaaggccca aacagtgtca ccaagctgaa gtacacgggt caccatgata taacctggta
      601 ccaaaccagc gttgaacaac atactgagaa ggacgaagat ggtaaaaaat ctgcgatact
      661 taaaggttgt ccgtgaaata gcgtaggcat aggttgttgt gataaagaca tttgtcaatg
      721 tcccaactac ggttacaaag acagagatga agagggcttg taggatttta tccttaaact
      781 gtgccaaaaa ctcaaaaccg tctaagccaa attgggatgg gaagaagcta tagccgtatt
      841 ggaggaggct tttctcgtct gtcactgaaa taatgataac gaatacaaaa ggtaggatac
      901 aagagagggc aatcaaaccc gaaatgatac tgaagaagat atctgctttc ttactgaagg
      961 agtgaatgcc gacattatca attttttctt ttttaatttt cttttttgcc atattctcct
     1021 cctttctaga acaaagctga gtttggatcg actcgtcttg caagcaagtt tgataggata
     1081 accagaatca aaccaacaac ggattggtaa agaccggctg ctgcagccat accgatatct
     1141 gctgtctgag tcaaaccatt aaaaacatat acgtccaaaa cgttggttac attgtaaagc
     1201 tgaccagcat tgtgtgggat ttgatagaag agaccgaagt ctgcgcggaa gatatttccg
     1261 actgcaagga tggtcaatac agttacaagc ggagtcaact gaggaatggt tacgttgcga
     1321 atacgttgcc acttgctagc tccgtccact gtcgctgctt cgtagtaggt tggatcaatt
     1381 cccatgatcg tcgcatagta catgacactg ctatatccaa aacctttcca aatacctagg
     1441 aaaagtagga gatagggcca gatgcccagg tcagcgtaga aattgacttc tttgagacca
     1501 agactttcca atagatgatt gaacacccct ttatcaatat ttaggaaggc atctgtaaag
     1561 aaactgatga taacccaaga caagaagtaa gggaacaaca tagaagtttg gaaaatcttc
     1621 accattctct tagaacggag ctcgctgagg ataatggcaa tccctacaga tacaactaaa
     1681 cctagaaaga taaagccaag attgtagagg acagtatttc gtgtgataat aaaggcgtct
     1741 cttgaactaa ataagaatct gaaattatcg agtccgaccc atttactatt tatgatacta
     1801 tctatgaaac cattactggt catgtggtag tctttgaagg caaccacgtt cccaaatact
     1861 ggaatgtaaa agaatagaat caaccagagt gcccctggca aaaccatcaa gagaaagatc
     1921 cagttgtctc tcaatgtttt tgaaaacttt ttcataattt cctccctttt tattttgata
     1981 tccatctaaa aattcttttt tagacttttg ataacgatta cattattagt atactcctat
     2041 ttgcaggtta ggttaaactc ctaattatag aaaaaactcc acaaattatg tagcagattt
     2101 aaaactttat caccactatc aaacaaatgt cctaaatcaa ttgtttattt tatctctatt
     2161 agcccagtga tggcgtcact ctgttataag catccaacaa cggggtatac tgaaaaatct
     2221 ccagactagg gaactcagag atagttccta atctggagat ttttaatatg ttattaggcg
     2281 tttgctttca acttagcaat aacctcttta agattatcaa tcacctctgc ttcagtatgc
     2341 tcagagcctt tttcatctgc caagaacaaa actgcttttt gaagttcttt ttgagagttt
     2401 tcaaggacat ccttatctac tgtttcaagg tttgagtctt taagaagttt acttaattcc
     2461 ttggctaatt tcttgagttt gatttgcaga ctcatcttct cctgctgttt ctttgcccgc
     2521 tgtttgtcct ccatccttag ttgctgactg gctttcctta atggactcta gggaagcaat
     2581 ggcatctttg actgtttgca agatatcacg taaaccttgc tctgtcaaac tatcatctgc
     2641 aaaagctttc ttagcctctg ccaaaaccag acgtgctgaa tctgtggtag gattcgatac
     2701 acctgtcaat gatctcaaaa gattttctaa ggtttgagtc tgcttactaa tactagacta
     2761 aaatcaaaaa gcattatata acagtgatat gaaatcaact aaagaagaaa tccaaaccat
     2821 caaaacactt ttaaaagact ctcgtacagc taaatatcat aaacgccttc aaatcgttct
     2881 atagtaaaat gaaacaagaa cagggtaaat cgatcaggac agtcaaatcg atttctaaca
     2941 atgttttaga agtagaggtg tactattcta gtttcaatcc attatatttc gtctgatggg
     3001 caaatcttat aaagagatta tagaactttt atagtagttt gaaataagat gtgaacaact
     3061 ctatcaggaa agtcaaatta atttatagaa atattttagc agccaaggtg tactgttata
     3121 gattcaatac actatacttg gtggtttagc tctcctgttt tctcttttag ctttaaccag
     3181 ccataaatgg tattacgtga gatttggaaa acgtgtgatg cttctgttat actacctgtt
     3241 cgctcacaat aagagagaac ttttttacga aaatctattg aatatgccat aaaagattat
     3301 accacattgt gtactatttt tggttcattt tactatatca ctttggacta tatcggtcta
     3361 gttatgtcta aacgattata gctggatact aagagaaatc aaaaaagcac taagagagaa
     3421 cgtcccaaag tgcttaatat caaggcctca aagcctatct tatttaataa aatattacaa
     3481 cattttgttg tagaattcaa cgacaagtgc ttcgttgatt tctgggttga tttcgtcgcg
     3541 ttctggcaag cgagtcaatg aaccttccaa cttttcagcg tcgaatgata cgaatgctgg
     3601 acgtccaaga gtagcttcta ctgcttcaag gattgctgga actttcaatg atttttcacg
     3661 aactgagatc acttgacctg gagttacgcg gtatgatggg atatcaacgc gtttcccgtc
     3721 aacaaggatg tgaccgtggt ttacgaattg acgagcttga cgacgagtag tcgcgagacc
     3781 aagacggtaa acaacgttat ccaaacgacg ttccaaaaga agcataaagt tgaaacctag
     3841 gattccgcct ttgatttttg tagcttgtac gaacaagtta cggaattgtt tttcacctac
     3901 accgtaagtg aaacgaagtt tttgtttttc agccaattgc aaaccgtatt ctgacaattt
     3961 agaacggttg tttggtccgt gttgtcctgg tacgtagtta cgacgtgcca attctttacc
     4021 tgtacctgta agtgaaaggc caaggcgacg agcttgtttc caagatggtc ctgtataacg
     4081 tgacatatga atgtcctcct gatataaata atatttcggt ggaaatagtc acttagaaag
     4141 ccctgattcg tgcagatgcc cttcgcctaa acagccaagg ttacttgtca taagacacct
     4201 gttgacgagc ttcatgcttt cctgctgata tttcacacaa aatctattct accatgaata
     4261 gctggatttg taaaggggtt ttaggcttta ttttcactac cagagaggtt gagaacgagg
     4321 gtaaaggtac ttcctagacc gtactggctg ctgactgtga tttccccacc caattgatgg
     4381 gccaattcac gcgcaatcgc aagtcctaat ccatgaccac ctgtcttcat gttacgcgaa
     4441 gtttcgacac gataaagacg tttgaaaata ttttccaaat cctctggggc aataccctgc
     4501 ccttcatcgg tcacactgat tgaaagctgg tccttctcca gcttagccac cacttccagc
     4561 ttggttcctg gagcagaata tttaaaagcg ttatcgacca gattcaccaa gatacgagaa
     4621 agcttagcat aatctccctc aatccgggca gactctggga ttacctgcaa gtggacatct
     4681 cttctctcct gctcaatcaa aaactgaaat tcactcatgc actcaattaa gagcttgtcc
     4741 agaaaaatac tgtctttact ggtagtttcc acctgatttc tagctgtgtt tagggtcaaa
     4801 aaattcaact cctcaaccag tttattgagc ctctccgtct ggcgtccaat ggttgctaga
     4861 taatgagctt gctccgactc cttgataatc ccatccaaaa tcccttctac cgtcgcttga
     4921 atcgaagtga taggggtctt aatatcatgc gacaactggg caatcatcaa gcccttttct
     4981 cgttcgcttt cttccaagga atcaaagctt acctgcaaat catgggacat ctcattaaaa
     5041 gtttgcccta attgctgaaa ttctacagga ccttgaacct ccaaatttga aggaaaatcc
     5101 ttggccgcta cccgcttggc atgctccttg agtttgccca acgacgtaaa gactggcaat
     5161 aggagaaaga gactaatccc agcaccgaca aggctggcaa cgatggtcat cccaagcaaa
     5221 aagtaaatct cgcctttcgc aatcagcatt ttttgaacag cccaaaaaac aaccaaaatg
     5281 gttaagaggg ttgaaataat atatccaacc aaaatataac tttttagttt catgtttgtc
     5341 ctctcggttt ctctatctta tatcccaacc cccaaactgt cttaatagtg ggcgtttggt
     5401 cactactata ttttgccagc tcctgtcgaa gagcatggat atgcacattc aaggtattgg
     5461 tgtcatccac gtagtcttct ttccagatct tttcatagag gtctgtcttg gagaaaactc
     5521 gctctggatt gctagctaaa atccacagca attcaaatga tttaacagtt aaatccagca
     5581 tttcttctcc tatttgaact tcatgactac tatgattcat ttttagattg ccaagggaaa
     5641 tcagctctgt ttcgccccca cgatgaaggc ggcgcaaaat attgtggaca cgcaaaacca
     5701 gctcacgtgg gctaaaaggc ttagcaataa aatcatctgc tcccaagctc aggccgtaaa
     5761 tcttgtcctg ttcactggtc ttagcagtaa taaataggaa aggctgctct ggtgataagt
     5821 attgaacctc actgattaaa tcataaccat ccatccgagg catcatgaca tctgtgataa
     5881 tcaaatcaat cggttttttc ttgaacagct ctagcgcttc cagtccatca tgggctacca
     5941 agacctgata acctgcctga attaagtatc tctgatgaat atctgtgatt tctacctcgt
     6001 cgtcaacgag taaaattgtc tttcccatct gtctctcctt tgataaaaac agtgctatac
     6061 tgctttaagt ataacactat ttttattgtt ttacgatgat tttaatctgc tattgaaaga
     6121 aagtcctgat tgctgattga gttgttaaac taatcttagt cagcccacta tccatcattt
     6181 atttccttag ctgattagtt ttcttctttt ttgtttttca aacctagacc accgagtaaa
     6241 cctgcaagcg caagcccaag gaaaccaata cttgccattg atgtttgagt ctcaccagta
     6301 tttggtagca tagctttatc ctctgacatc atcgtatcag acatcttgtt agcagaagca
     6361 gccatgtttt cacctgccat cgtgttggta gaacttgtca tggtgtcagc aggcatgcta
     6421 tctgtagtac ctgtagcatg attgtgattc atcggagtca cgccagaacc agagttagaa
     6481 ggtgataatg aaccatttgc tgtgtctgat gtttctttaa catttat
//
LOCUS       DAVAAL010000102         6481 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVAAL010000102 DAVAAL010000000
VERSION     DAVAAL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.103"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(114..824)
                     /gene="treR"
                     /locus_tag="VQM60_001611"
     CDS             complement(114..824)
                     /gene="treR"
                     /locus_tag="VQM60_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET1081465.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            1009..2976
                     /gene="treP"
                     /locus_tag="VQM60_001612"
     CDS             1009..2976
                     /gene="treP"
                     /locus_tag="VQM60_001612"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET1081466.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            3154..4779
                     /gene="treC"
                     /locus_tag="VQM60_001613"
     CDS             3154..4779
                     /gene="treC"
                     /locus_tag="VQM60_001613"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET1081467.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            5035..5283
                     /locus_tag="VQM60_001614"
     CDS             5035..5283
                     /locus_tag="VQM60_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET1081468.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            5490..6284
                     /gene="racE"
                     /locus_tag="VQM60_001615"
     CDS             5490..6284
                     /gene="racE"
                     /locus_tag="VQM60_001615"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET1081469.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            6281..>6481
                     /locus_tag="VQM60_001616"
     CDS             6281..>6481
                     /locus_tag="VQM60_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="HAM1-like protein; Rec-dependent growth; RgdB;
                     yggV; it is suspected that this protein functions to
                     remove misincorporated bases such as xanthine or
                     hypoxanthine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HET1081470.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLF"
BASE COUNT         1777 a         1291 c         1452 g         1961 t
ORIGIN      
        1 tcttatggga ctttttttct acaataaaat aggctccata atatctatag ggggggttac
       61 ccactacaaa tattatagag ccgaaaaagg ctgagatctg gtgtctcagt cttttatttc
      121 ttgcgttttg caaaatctac aaatctaaat ttctctaact tatggcgact ttcggtaaac
      181 tgaaattggc gtccgtcttg gagatagact tttgatttaa tcgaaacgac ataagggtct
      241 ttgccaatgt ccatgagaat cttgtctcgg tcgcttgagt ggtcaatggt gatttccttc
      301 tgagcataat caataaggag tttgaggcca ttttctatat aagaatagat agactgctca
      361 gcaatttggc gagtgagatt tgggatgagt tccatatcca gatagtctgt atccagaacg
      421 gataccagat catccaccac acgctggcgg accaccttcc aaaccatccg aaactctggg
      481 aaaccggtta tcagtgagga ttttttatca ataataatct tgtccagact gaccacgttg
      541 gttttagagc gcagtccaag ttctttaact agttcttggt agctggttag gttggataca
      601 gggaaattga cggtttcttc tttgacgact tgagaacctt gccctcttat ctttttgatc
      661 aatccttcct cttggaggag agacagggcc tttcggacgg tatcacgact cacttgatat
      721 tgctccataa ggtcgtgctc gctaggaagg aaatctccga cagcgtaagt ctcgttttga
      781 atggtttctt ggatttgctt aaataattgt tggtatttct tcattttgcc tgcttaaata
      841 ggtttttagt ctagtttaat gaacacctgt atctatttta ccatacaagt gctagaaaat
      901 ttacaaaaaa acaccctttt tataccaaac aagttgcaga caagtttggt atcgtttaca
      961 atatacttat caaatcaaac ttgctttgac aagtataagg agaatcaaat gggaaaattt
     1021 gaacaagaag ccaaagatct gcttcaggca attggaggca aagaaaatgt gactgccgta
     1081 actcactgtg cgacacggat gcgctttgtt ttaggagatg ataagaaggc taatgttaaa
     1141 gctatcgagt caattccagc tgttaaagga acttttacaa atgcaggtca atttcaggta
     1201 atcattggaa atgacgtgcc catcttttat aatgatttta cagccgtttc aggtattgag
     1261 ggtgtttcta aagaagcagc caagtctgca gctaagagta atcaaaacgt ggtccaacgt
     1321 gttatgacca ctctggcgga aatctttact ccgattattc cagctttgat tgttggtgga
     1381 ttgatcctcg gtttccgtaa tgtcttggaa ggtgtccatt ggtcgatgtt ggatggcaag
     1441 accatcacag aatcctctca gttttgggca ggggttaatc acttcctctg gttgcctggt
     1501 gaagctatct tccagttctt accagtaggg attacttggt ctgtttctcg taagatggga
     1561 accagccaaa ttttgggaat tgttctcgga atctgtttgg tatcgcctca gttgctcaat
     1621 gcctatgcgg ttgcttcaac gccagcagct gatatcgcag caaactgggt ttggaatttt
     1681 ggttatttta ctgttaatcg tattggttac caagcccaag ttatcccagc cttgcttgca
     1741 ggtttgagtc tgtcttatct tgaaattttc tggcgcaaac atatcccaga agtcatttcc
     1801 atgatttttg tacctttttt gtcattgatt ccagccttga ttttggctca tactgttttg
     1861 ggaccaatcg gttggacaat tggacaagga ctttcatcag ttgtcttggc aggtttaact
     1921 ggtccagtta aatggctctt cggtgcaatt tttggtgccc tctacgctcc atttgtcatc
     1981 acaggtctgc accatatgac caatgccatt gatacacaat tgattgcgga tgctggtggc
     2041 actgccctct ggccaatgat tgctctttct aatattgctc aaggctcagc cgtgtttgcc
     2101 tattatttca tgcatcgcca tgatgagcgt gaggctcagg tttcacttcc tgcaaccatt
     2161 tcagcctatc tcggtgttac agaaccagct ctttttgggg ttaacgtaaa atatatttat
     2221 ccatttgttg ctgggatgac tggttcagcc cttgcaggca tgttatccgt tacttttaat
     2281 gtaactgcgg cttctattgg tatcggtggt ttgccaggta ttctctctat tcaacctcaa
     2341 tacatgctgc catttgcagg aactatgcta gttgcgattg ttgttccaat gctcttgact
     2401 ttcttcttcc gcaaggctgg tctctttaca aaaacagagg gcgatacgaa cttgcaggca
     2461 gaattcgttg ctcaagaaga agcagaattt gtgaaccatg aaccagtaga acttacttcg
     2521 gtagaaatta tcagcccact aactggccaa gtgaaagaat tgagtcaagc gacggatcct
     2581 atttttgcat caggtgtcat ggggcaaggt ctggtcattg aaccaagcca aggtgagttg
     2641 acctctccag ttaatgggac agtgacggtt cttttcccta ccaagcatgc catcggcatt
     2701 gtctctgacg agggagttga attgctcatc cacatcggta tggatacagt aggtcttgat
     2761 ggcaaaggtt ttgaaagtct tgtagtccaa ggagatcacg ttacagttgg tcagcaactg
     2821 attcgttttg atatggatgt cattaaggct gcaggtctgg tgacagaaac tcctgttatc
     2881 atcaccaacc aagatgctta tacagcgact attcccggaa cttatccgac aacgatccaa
     2941 gctggagcat ctctcatggt cgctacacga atctaatact cttcgaaaat ctcttcaaac
     3001 cacgtcagct ctatctgcaa cctcaaaata gtgttttgag caacctgtgg ctagcttcct
     3061 agtttgttct ttgattttca ttgagtataa ttgaaatagc acaaataggg acggaagact
     3121 gcttcctccc ttttatcaga aaaggagaaa cagatgacac ttgataaagg aaaagtagtc
     3181 tatcaaatct atccaaaatc ttacaaagac accactgaaa acggtattgg ggatttccgt
     3241 gggattatcg aaaaaattcc ctatttagcc aaactaggtg tggatatggt ctggctcaat
     3301 ccattctatc caagccctca acgggataat ggttacgata tttcagatta tatggcagtg
     3361 gatcctcttt ttggtgatat ggctgatttt gaggaaatgg tgtgtgtcgg taaagagcac
     3421 aagattgact ttatgctgga catggtactt aatcattgtt cgacagagca tgaatggttt
     3481 cagaaagccc tagctggtga taagtattat caagactttt tcttcatcca agaccaacca
     3541 acagattggc agtctaagtt tggtggttct gcatggacgc ctttcgggga tactgggaaa
     3601 tactatcttc acctatttga tgagacccag gctgacctta actggcgcaa ttccaatgtc
     3661 cgtaaggagc ttttcaaggt tgttaatttc tggcgcgaca agggtatcaa aggtttccga
     3721 tttgatgtga tcaatttgat cggtaaggac gaggtttcag tggactgtcc tgaaaatgaa
     3781 gggaagccag cttatacaga caaacccatc gttcataact atttgcgtat gatgaaccaa
     3841 gccacttttg gatctgacga tagctttatg acagttgggg aaatgtcttc taccaccatg
     3901 gaaaactgtg tcctttattc gtcgcctgat cgtcaggaat tatccatgac ctttaatttt
     3961 catcacctca aggtggacta taaggatgga caaaagtgga ccttggctcc ctttgatttt
     4021 gaagagttga agagtcttta ccatagttgg ggtaaggaaa tgagtgataa agacggttgg
     4081 agtgccctct tttggaacaa tcacgatcaa ccacgtgcct tgaaccgttt tgtcgatatt
     4141 caaaacttcc gcaaggaagg agccaccatg ctagcggcca gcattcacct gtcacgtggg
     4201 acaccttata tctacatggg tgaggaaatc gggatgattg acccagacta tgattctatg
     4261 gctgattatg tggatgtcga atcgcttaat gcctatcaga tgctcttaga agaaggaaag
     4321 agtcagcaag aagccttcca gattattcaa gctaagtcgc gtgataattc acgcattccc
     4381 atgcagtggg atgcttcgga aaatgcagga ttttcaacag gcactccttg gttgaaagct
     4441 ggtaaatcct ataaagacat caatgtagaa aatgaaatcc agggtccgat ttttaccttc
     4501 tatcaagact tgattcgact gcgtaaggaa atgccaatca tttcagaagg aagttacaaa
     4561 ccagcctttg aagatagcaa acaagtctac gcttttgaac gccagtttga ggatcaaaag
     4621 ttactagtgc tcaataactt ttatgctaaa gaagtggaaa tcgacttgcc agcagtctac
     4681 caaaatggac agattttgat atcaaactat gaagatgcag aagtgtctga gaaaattcta
     4741 ctcaaaccct atcaaacact ggctatttat gtaaattaat actcttcgca aatctcttca
     4801 aaccgtgtca gttttatctg caacctcaaa gcggtgcttt gagcaacctg tggctagctt
     4861 cctagtttgc tctttgattt tcattgagta taaacttagc aaggagacct ctttttcaga
     4921 aaagcagggt tctttttgct gtctattcca tccttgaaag tgtagaacaa actggtcagg
     4981 gtggctagtt tgtggtataa tgaaagggat tcaaaaagaa acaggagaaa aatgatggat
     5041 ttacttttag caattgtatt gattgtgcta gcttttctag gaggagctct tggaggaatg
     5101 tacttggttc gtaagcaaat cgaaaaagaa tttgctgaca acccacgttt gaatgctgaa
     5161 gcagttcgta ctcttttgag tgcaaatggt caaaaaccaa gcgaagctaa ggtacaacaa
     5221 gtttaccacc aaatcatccg ccaacaaaag gcagcccttg ctaacaataa aaagaaaaaa
     5281 taaataagga aaagtctggg atgaaagttc cagacttctc actatgttgg ttatggttta
     5341 gggatgagac tttttccttg cattctattg atatttgatt atgattaaga gagaggtagt
     5401 tgacattcta tcaagctgtc ggtcagttcg ttcagtacaa ggaatcataa tgaacgatca
     5461 atcagaaaaa agactagaaa gaagactgta tggataatcg accaattggt tttttggatt
     5521 cgggtgtcgg gggcttgacc gttgtgcgcg agctcatgcg ccagcttccc catgaagaaa
     5581 tcgtctatat tggagattcg gcgcgggcgc cctatggccc ccgtcctgct gagcaaattc
     5641 gtgaatatac ttggcagctg gtcaactttc tcttgaccaa ggatgtcaaa atgattgtca
     5701 ttgcttgtaa cactgcgact gcggtcgtct gggaagaaat caaggctcaa ctagatattc
     5761 ctgtcttggg tgtaattttg ccaggagctt cggcagccat caagtccagt caaggtggga
     5821 aaatcggagt gattggaacg cccatgacgg tacaatcaga catataccgt cagaaaatcc
     5881 atgatctgga tcccgactta caggtggaga gcttggcctg tcccaagttt gctcccttgg
     5941 ttgagtcagg tgccctgtca accagtgtta ccaagaaggt ggtctatgaa accctgcgtc
     6001 ccttggttgg aaaggtggat agcctgattt tgggctgtac tcattatcca ctccttcgcc
     6061 ctattatcca aaatgtgatg gggccaaagg ttcagctcat cgatagtggg gcagagtgcg
     6121 tacgggatat ctcagtctta ctcaattatt ttgaaatcaa tcgtggtcgc gatgctggac
     6181 cactccatca ccgtttttac acaacagcca gtagccaaag ttttgcacaa attggtgaag
     6241 aatggctgga aaaagagatt catgtggagc atgtagaatt atgacaaata aaatttatga
     6301 atataaggat gaccaggact ggtatgttgg gtcttatagt atttttggtg gcgttaacag
     6361 tttgagcgac tataagacag attttcctct gtttgaattc tccaaaatat ttggagatga
     6421 agagtatggt ttcccgcttt cagttactgt tttacgctat ggttctacct accgtttgtt
     6481 c
//
LOCUS       DAVAAL010000103         6359 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVAAL010000103 DAVAAL010000000
VERSION     DAVAAL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.104"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..1232
                     /locus_tag="VQM60_001617"
     CDS             <1..1232
                     /locus_tag="VQM60_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET1081471.1"
                     /translation="LVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLTTQVIYPVKPA
                     SARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPEVSYVIVNESG
                     ASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGVGQYQHDVSQK
                     KLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYREEEGKITSRSQ
                     IKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCLDIKDLNEEAQ
                     SKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQDVLDIKDLVVG
                     QKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGDLVSVWVKKID
                     TEREKVNLSLLAPNETD"
     gene            1219..1668
                     /locus_tag="VQM60_001618"
     CDS             1219..1668
                     /locus_tag="VQM60_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET1081472.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            1727..1999
                     /locus_tag="VQM60_001619"
     CDS             1727..1999
                     /locus_tag="VQM60_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET1081473.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(2353..2544)
                     /locus_tag="VQM60_001620"
     CDS             complement(2353..2544)
                     /locus_tag="VQM60_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081474.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            2972..3730
                     /locus_tag="VQM60_001621"
     CDS             2972..3730
                     /locus_tag="VQM60_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081475.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            3732..5720
                     /locus_tag="VQM60_001622"
     CDS             3732..5720
                     /locus_tag="VQM60_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1081476.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(5763..6341)
                     /locus_tag="VQM60_001623"
     CDS             complement(5763..6341)
                     /locus_tag="VQM60_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET1081477.1"
                     /translation="MDSNFAGRLNILRAGVLDANDGIISIAGGEYVSVSTPKDTEEAA
                     VSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLLTNKIFLKNPLKALVEEKYGIEY
                     EEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYRIPATVLIVGVALLLTGYTSARL
                     GKAPTKTAMIRNLAIGLLTMGVTFLLGQLFSI"
BASE COUNT         1898 a         1235 c         1312 g         1914 t
ORIGIN      
        1 tcttggttgc tccactgaaa gggcgcgtgg ttcttgggtt tgacccagcc tttcgtacag
       61 gtgccaagct agctgtcgtg gatgcaacag gaaaaatgct gacaactcag gttatttatc
      121 ctgttaaacc agcatcagct cgtcaaatcg aagaagccaa gaaagattta gcagatttaa
      181 ttggtcaata cggtgtagag attattgcca ttggaaatgg aacggccagt cgtgaaagtg
      241 aagcttttgt agcggaagtt ctgaaagatt tccctgaagt cagctatgtt atcgttaatg
      301 aaagtggtgc ttctgtctat tctgccagcg aacttgctcg tcaggagttt ccagacttga
      361 ccgttgaaaa acgctctgcc atttctatcg cccgtcgttt gcaagatcct cttgcggaat
      421 tggtcaaaat cgatcctaag tcaattggtg tcggtcaata ccaacacgat gtcagtcaga
      481 agaaactatc tgagagtctg gactttgttg tcgatacagt ggttaaccaa gttggtgtca
      541 atgtcaatac agctagccca gctcttcttt cacacgtagc tggactcaat aaaaccattt
      601 ctgaaaatat cgtcaaatac cgtgaggaag agggaaaaat cacttcacgc tctcaaatca
      661 agaaagttcc tcgtttgggg gccaaggctt ttgaacaggc tgctggtttc ctccgtatcc
      721 ctgaaagtag caatatcctt gataatacag gagttcaccc agaaaattac gctgccgtta
      781 aggaactctt caaatgcttg gacattaagg atctgaatga agaggcccaa agtaaactca
      841 agtcgctttc agtcaaggaa atggcgcaag agttagacct tggtccagaa actcttaaag
      901 atatcattgc agatcttctc aaaccaggtc gagatttccg tgattccttt gatgctccag
      961 ttctccgtca agatgtccta gatatcaagg acttagtggt tggacaaaag ctagaaggag
     1021 tggtgcgtaa tgttgttgat ttcggtgctt ttgttgatat cggaattcat gaggacggtc
     1081 tcatccatat ttcccatatg agtcgcaagt ttatcaaaca ccctagtcaa gtggtggcag
     1141 ttggagattt ggtgagcgtt tgggttaaga aaattgatac tgaacgtgaa aaagttaatc
     1201 tgtctctcct agctccaaat gaaactgact gattatgtca agcaggtttc actagaagac
     1261 ttcggcagac ctttcatcca tcatgtccag tggaataggc gtctacgttc gacaggtggg
     1321 cgatttttcc ccaaagatgg gcatctggat tttaatccca aggtttatca gaaactgggt
     1381 atggaggttt ttaggaaaat tgtccgacat gaactctgtc attatcacct ttatttccaa
     1441 gggaagggct atcaacacaa ggatcgggat tttaaggaac tgttgaaagc agtggatgga
     1501 ttaaggtttg taccatcctt gcccaatagt aactctaaac cactcaagct ttatcgttgt
     1561 caatcctgcc agcaaaggta tcagcgcaag cgtaggattg ataccaaacg ctatcgctgt
     1621 ggactttgtc gaggtaaatt gcttctgata aatcagcctg aggactgatg aaagccgagc
     1681 ctccccgtga tatactatgt ctaacctagc agaaagagga aatatcatga acagtaaatt
     1741 ttataaaatg agacgcaatc gcatgatttc aggagttctg gcaggtctgt cagacaagtg
     1801 gaattttgat gttaccctag ttcgtttctt gtttgccatt tttacagttg caaactttgg
     1861 acttggcgtg attatctata tcatcctagc ttctatcatg cctaccaagg aagaaatcga
     1921 agcagaaatg tacggaacag gcccacgtaa acgcaaagaa gcccaagcca ttgacgacaa
     1981 tgaaggctgg ttttggtgag gttctacaga agatttggaa taagcaaaaa gctttaaatc
     2041 aatagtctaa actgcttgat ttaaagcttt tttactttta tactcaataa aaatcaaagt
     2101 gcaaactagg aagccagcca caggctgtaa tttagtcggc aatgtgaagt tgacatagtt
     2161 tgcactttga tttttgaata gtattaataa tatttcgcca gcttcatcca caaactatag
     2221 tggattggaa ctagaatagg acacctctac ttctaaaaca ttgttagaaa tcgatttgac
     2281 tatcctgatc gatttgtcct gttcttattt cattttatta taaaagcagt aatttaaact
     2341 cagtttttta atctattttt gctgctgaga caatacattt ttggaaaaat ggtggatgct
     2401 gataacaact gataaccagc aaataacata agcagtgccg gtaaattctg taccgataaa
     2461 gtgtgtgtgt tcaactaaga gctgaccgat gaatggagag agcagataga gaagtccgat
     2521 gagtatatgt tctttgtgtt tcattaaagc gcaccttctt tccttatagg ttgaacgact
     2581 tgcaagtgtt aaattgaatg gtatgttatc taggtaacct tactaaaatg ttagttcccg
     2641 cttcctcctt ttcaaagtaa gccttttcac acttatattt tacaacttta aaaataggaa
     2701 atcaaatcat tttctaagta attttgctta tatttcaccc aatcttacaa aaatgtaaga
     2761 ttaaagtctc ttttgtaagg ttaggttatg gcaagcgatc tttttttgat gtaaactaga
     2821 ctcataaaga acaaaggagt aacatcatga aacaaatctt acataagaaa actagaaaac
     2881 caagtcacaa agaaattgag cgcgttcagc tgggatgcac tatgatgcaa gctacatttc
     2941 atttgatggg atattaagaa aggagattct catgacactt ttagatgtaa aacacgttca
     3001 aaaaatttat aaaacacgtt ttcagggcaa ccaagtagaa gccctcaagg atattcactt
     3061 taccgtcgaa aagggtgact acgttgccat catgggtgag tctggttctg gtaaatcaac
     3121 tcttctcaat attctagcta tgctggataa accaagtcgt ggtcaggttt acttgaatgg
     3181 aactgacacc gcaaccatta aaaattcaca ggcctctagc ttccgtcgtg aaaagttggg
     3241 ctttgtcttc caagacttta acttgctaga tactctatct gtaaaggaca atatcttgct
     3301 tccgcttgtc ttgtcaagaa gacctattac agagatgatg aagaaattgg tggtgacagc
     3361 tgagaatctg ggaataaacc aattacaaga gaagtaccct tacgagattt ctggtggcca
     3421 gaaacagcgt gtagcagtag cacgcgccat catcactgaa cctgaaattc tccttgcgga
     3481 cgaaccgaca ggagctcttg actccaagtc atctgcagcc ttacttgatg tctttgatga
     3541 aatcaatgag cgcggccaaa ccattctcat ggtaacccac tcaacagcag cagctagcag
     3601 agctaaacgt gtactcttta tcaaagacgg cattctttac aatcaaatct accgtggaga
     3661 gaagacagaa cgtcagatgt tccaagaaat ctctgatacc ttgactgtca tggcaagcga
     3721 ggtgaattag tatgtttcga ttaaccaata agttagcggt atcgaacttg attaaaaacc
     3781 gcaaactcta ctatcctttt gcgctggctg ttctcttggc agtcactgtc acctatctct
     3841 tttactctct aaccttcaat cctaagattg cggaaatccg tggaggaaca accattcagg
     3901 ctacacttgg atttggtatg tttgtcgtca cccttgcgtc agccattatc gttctctatg
     3961 ccaatagttt tgtcatgaag aaccgttcca aggaactagg aatttatggc atgttgggct
     4021 tggagaagcg tcatcttatc agtatgacct ttaaggagtt agtggtattt gggattctaa
     4081 ctgttggagc gggtatcggt attggagcct tgtttgacaa gttaattttc gctttcctgc
     4141 tcaaactaat gaaattgaag gttgagctgg ttgctacctt ccagacgaaa gttgtcatta
     4201 cagtgcttgt tgtcttcggt ttgattttcc taggcctcat gttcctgaat gcccttcgaa
     4261 tcgcccgtat gaatgccctc cagctctctc gtgagaaagc tagtggagag aaaaaaggtc
     4321 gcttccttcc tctccaaacc attcttggtt ccataagttt aggaattggc tattatcttg
     4381 cccttacggt aaaagatcct cttacagcct taacaacctt cttcatagct gttttactgg
     4441 ttatctttgg gacttatctc ttgtttaatg cagggattac cgttttcctc caaatcttaa
     4501 agaaaaataa gaaatactat taccaaccaa ataacctcat atctgtttct aacttgattt
     4561 tccgtatgaa gaaaaatgca gttggactag ctaccatcgc tatcttgtca acaatggttt
     4621 tggtaaccat gtcagcagcg acaagcattt tcaattcctc agaaagcttt aaaaaagttc
     4681 taaatcctca tgattttggg gtttcagggc aaaatgttga aaaagaagac ttggacaaac
     4741 tcttgagcca gtttgcaagt gacaaaggtt ataagattaa agaaaaagaa gtatttcgtt
     4801 acacttactt tgctgttgcg aatcaagaag gaaccaagtt aactattttt gaaaaaggac
     4861 aaaaccgtgt ccaaccaaaa acagttttca tggtatttga ccaaaaagat tatgaaaata
     4921 tgactggtca aaaactgtct ctatcaggaa atgaggtcgg actctttgca aagaatgagg
     4981 gagttaaaga acagaaagct ctaactctaa atgatcatca attttctgta aaagaagaat
     5041 ttactaaaga ttttattgtc aaccatgttc caaatcagtt taatattttg actgctgatt
     5101 acaattacct tgttgtacct gatttacaag cctttttgga tcaattccca gattcggcta
     5161 tctataatca gttttacggt ggtatgaatg taaatgccag tgaagcagaa caactcaagg
     5221 tcgctgagga gtatgaaaaa tacttacaaa agtttaatgc tcaattaaac actgaaggta
     5281 actatgtgta tggtagcact ctagcagatg ctagtgctca gatgagtgcc ctctttggtg
     5341 gtgtcttctt tatcggtatt ttcttatcca ttatctttat ggtcggaacc gttctggtca
     5401 tctactacaa acaaatttct gaaggatatg aagaccgtga gcgctttatt atcttgcaga
     5461 aagtcggttt agatcaaaag caaatcaagc aaaccatcaa caaacaggtt ttaactgtat
     5521 tcttccttcc tttgctcttt gccttcctac atctagcctt tgcctaccat atgcttagtc
     5581 tgattttaaa agtgattggt gtactggata cgactatgat gttgattgtg accttgtcta
     5641 tctgcgctat cttcctcatc gcctatgtgc tgattttcat gattacttca agaagttatc
     5701 gtaagattgt ccaaatgtaa aaaaagatac ctcgacttca aaatcgaggt atttcttgtt
     5761 ttctaaatgc tgaaaagttg tccgagcagg aaggtaactc ccatagttaa gagaccaata
     5821 gcaaggttcc gaatcatagc tgttttggtt ggggcttttc caagtctagc acttgtgtag
     5881 ccagtgagaa gaagggctac accgacgata aggacggtag cagggatgcg gtaatcactt
     5941 ggaaaaatgg tcactgacag cattggaggc aaacttctaa ggaaaaaggc aacgaagcta
     6001 gaaatggcag cgtgccaagg attggtaaat tcttcatact caatcccata tttttcctct
     6061 accagagcct tgagtggatt tttaagaaag atcttattag tcaagagttg ggcagaagtt
     6121 ttgaattctc cattttggat ataagaagca tagagggatt ttttggctag ttccctatct
     6181 tggtctagca agagtttttc tcgcgaaacg gcagcttcct cggtatcttt tggagttgaa
     6241 acggatacat attctccacc agcaatggaa ataattccat cgttagcatc aagaacaccc
     6301 gcacgcagga tatttaaacg acctgcaaaa tttgaatcaa tttcgtgatt tgtttctga
//
LOCUS       DAVAAL010000104         6327 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVAAL010000104 DAVAAL010000000
VERSION     DAVAAL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.105"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(118..522)
                     /locus_tag="VQM60_001624"
     CDS             complement(118..522)
                     /locus_tag="VQM60_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET1081478.1"
                     /translation="MKKIFATKRNVFLVRLLLGQIPLVVSTYLFLSRQFLNFSLVFQF
                     LLVIINLASILATVYLTREMRIRKFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     AESYQQIIAYIGAVLCLLIMLLLIWGLKYYKK"
     gene            complement(552..740)
                     /locus_tag="VQM60_001625"
     CDS             complement(552..740)
                     /locus_tag="VQM60_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081479.1"
                     /translation="MAALNTSLTTNIKFGLVGTVGGRFAGYGMYVLGYPGDYLDITNH
                     RVVSAKTSHKSYQILEND"
     gene            complement(967..1542)
                     /locus_tag="VQM60_001626"
     CDS             complement(967..1542)
                     /locus_tag="VQM60_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET1081480.1"
                     /translation="MFVGFLVYVIVATVGYTINQGDYFQNEHLFIIAKTFLISLSVAY
                     AKWFDMISLRRLTKKEVLLFIASFLLCVLVNIGYHSLFTVSSGAGYQHLEAASTGISL
                     SFIASATVFGPILEEFVFRGILQGVVFENSWLGLVLTASLFSFLHAPYDFPSFIYYLF
                     GGFMLGFAYKKSQKLSVAILVYICYNCLSFL"
     gene            complement(1562..1666)
                     /locus_tag="VQM60_001627"
                     /pseudo
     CDS             complement(1562..1666)
                     /locus_tag="VQM60_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037603748.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2744..2953)
                     /locus_tag="VQM60_001628"
     CDS             complement(2744..2953)
                     /locus_tag="VQM60_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HET1081481.1"
                     /translation="MFNYKIVDNQELSNISGGGLGGDVVVGALSGAFQAGQSCIAGGP
                     QAYLICATGGAIVGGILAYGLRPPK"
     gene            complement(2969..3217)
                     /locus_tag="VQM60_001629"
     CDS             complement(2969..3217)
                     /locus_tag="VQM60_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HET1081482.1"
                     /translation="MNTKMMSQFSVMDNEMLASIEGGTDWGTVGKGAVYGAGIGVAMC
                     AVGGLLTGGSTWAMTAGCAWAGAKLGGSFTAIADNLWP"
     gene            3492..4750
                     /locus_tag="VQM60_001630"
                     /pseudo
     CDS             3492..4750
                     /locus_tag="VQM60_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            4761..6122
                     /locus_tag="VQM60_001631"
     CDS             4761..6122
                     /locus_tag="VQM60_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET1081483.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEITPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ETQLQRLEKQKEGLGILKQSLEKATDLFSSEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDTIVNKRARLPTGNPHQSILNRY
                     LIASQEQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLGNNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKILT
                     HSD"
     gene            6179..>6327
                     /gene="blpC"
                     /locus_tag="VQM60_001632"
     CDS             6179..>6327
                     /gene="blpC"
                     /locus_tag="VQM60_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET1081484.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDILYSLNIIKHNNTKG
                     LHHPI"
BASE COUNT         2148 a         1318 c          971 g         1890 t
ORIGIN      
        1 gtactattct agtttcaata tactatacta taccatattt tgtttcgcag ggaatctatt
       61 ataaaagggt aagtattgcg aaaacactta cccttttctt ttatacttca ttaagctcta
      121 ctttttataa tacttcaaac cccaaatgag caaaagcatg ataagcaagc agagaaccgc
      181 gccaatataa gcgattattt gttgatagga ttctgctgct gtgatccctc tatacaaaca
      241 tataatagac ataaaacctg tcaagccgat aaacatgagt tgattggttc taggactaac
      301 caaatcatca tcttcaaact ttcttatcct catttcccta gtgagataaa cagtagccaa
      361 aatagaagcc aagttaataa tcactaaaag aaattggaaa accaaggaaa aatttaaaaa
      421 ctgacgagat agaaatagat aagtagaaac aaccaaaggt atctgtccta acaacaacct
      481 cactaagaaa acattgcgtt ttgttgcaaa tatttttttc atacctaaac tccattacaa
      541 ttaaatgata tctaatcatt ttctaaaatt tgataacttt tgtgagatgt tttagcagaa
      601 acaactctat gattggtgat atctagataa tcacctgggt aaccaagaac atacatacca
      661 taaccagcga atcttcctcc aactgttcca accagaccaa acttgatatt agttgtgaga
      721 gaagtattaa gagccgccat aagaatgcgc acatgattat ccaaccaaac catataattt
      781 tttgaaaagc tacctcttcc caacctccta aacctccttc aactttcgca aaaatttcgg
      841 tatccataat ctcaaattgt gacatcattt catttaatgt tgttaatcta aacagtaata
      901 gtatccattt ccctattttt taagattttt tgtccaatat tatgtcatat tgattactta
      961 catatactac aggaatgata aacagttata acagatataa actaaaatag ccaccgatag
     1021 tttttgactc tttttataag caaagcccag catgaaacct ccaaatagat aatagataaa
     1081 cgaaggaaaa tcataaggag catgcaggaa agagaagaga gaggcagtca gtacaagccc
     1141 caaccaagaa ttttcaaaaa ccacaccttg aaggattcct ctgaaaacaa attcctccag
     1201 aatagggcca aaaactgtcg cgcttgcaat aaaggataag gaaattcctg tactagcagc
     1261 ctcaaggtgt tgatatcctg caccagaaga aactgtgaaa agactatgat aacctatatt
     1321 taccagcacg caaagaagaa agcttgcaat aaaaagtaga acttcttttt tcgttaacct
     1381 tcttagcgaa atcatatcaa accatttcgc ataggcaaca ctcagtgaaa tcaagaaagt
     1441 ctttgcaata ataaataggt gctcgttttg aaaataatca ccttgattga tggtatagcc
     1501 tactgtagcg actattacat agactaaaaa tccaacaaac acggcatgat atttattaaa
     1561 caggtttgag tcctacgctt aaataagctt caatttctcc tctttcagct tcagataagt
     1621 gattgtatta ttgatttgtg gtagaatagt ttgtggacat gttcatcttc ctttatactg
     1681 tttttcgcaa ctctagtata tcagatgaac atgtcttttg tgttgcactt cattttacaa
     1741 cagagcgaat atatttagtt gtaaaagtac caattatgat atttttcgct ctgcagaata
     1801 gaaatatcat ttgctttcaa aataatgatt aacacaatca tatttatttg ttacaaagat
     1861 ttcttaacta actcctctct caggctacat aagcgttcta ttcgtgtgtc ctttttttta
     1921 tgaacaatag tcaccgttta tattttcata ctaccatcct atcaaacaaa taatcaaaac
     1981 agacaattct actttcaatt gttcacccct tcgctccact tccactacag aagtttcctc
     2041 actactatgg gttcggctga cttctcatga ttcgttgtta ctacgctttt agcgctcatg
     2101 agacctcacg ggataagtcg tacatctttc ctcgtttact ctcgtgattt acgcatatag
     2161 gttacgacta cctttttgga ctttagagtt tgaggccccc ttatccgcta tatacgcctt
     2221 actatcacgt tcctgttcgt agagccacga tttcgctatc ccttcctctc cctgctacct
     2281 cacgatagtc aggcttggga atcgctattg ggttcaccgg tagcaggtgc ccaccgagga
     2341 cttacacctc agatatacga catgcccgtc gtacatcaaa aaagaaagga cgaaattcgt
     2401 cctttctcga tcttagattt tcttcaactc actacagttg acaatgagcc aaaggatagc
     2461 taataaccta ttgaagtgtc aaacgttgct aactccattt atcttcaaat ttaaataggc
     2521 acattatcgg agttatcagt aagaaaaaat ctaaccagtt cagataattc ataatatagg
     2581 atataatacc agccataacg aaaaggtaca aagctgtacg atatcctctc ttttggtttt
     2641 tatcagcctt ggtgttaaaa ataatgtaca cacaacaaat tgcaatgagt acaagctgaa
     2701 tcatagtttc atttaacata ttaacactca ttccttttta aaactatttt ggtggtctca
     2761 atccataagc taaaatgcca cctactatag cgccccctgt tgcgcaaatc aagtaagctt
     2821 gagggcctcc agcaatacaa gattgtcctg cctgaaatgc acccgacaag gctcctacaa
     2881 ctacatctcc tcccaatcca cctccagaaa tattacttaa ttcttgatta tctactattt
     2941 tgtaattaaa cataatttct attcccttct atggccaaag attatcagca attgcagtaa
     3001 aagaaccacc aagtttagct ccagcccaag cacagccagc agtcatagcc catgtggaac
     3061 caccagtaag gagtcctcct accgcacaca tagcaactcc gatacctgct ccatagacag
     3121 ctccttttcc tactgttccc caatcagttc ctccctcaat gctagccagc atttcattat
     3181 ccataacaga aaattgtgac atcatttttg tattcatgac aaatactcct tttttatttt
     3241 tgtcttgttg caactttgac aagtttagta tatcattttt tttttaaatt tttcatccaa
     3301 atcttgaatt gtcatcgaaa cgtcttgaat tagcttttca tttgaaacca cctctaaatg
     3361 tttaaaaaaa aaataatttc taatcacttt tttaccattc aggaagtttc aatgactatt
     3421 caagatttaa taaaatatga acttattttt atggcataat atacctatct actatatgaa
     3481 aggaattgcc aatgacttct tataaacgta catttgttcc tcaaatagat gcgagagact
     3541 gcggtgttgc tgccttagcc tcgattgcta aattctatgg ttcagatttt tctctagctc
     3601 acttgagaga acttgcaaag accaataaag aaggaacgac tgctcttggc attgtaaaag
     3661 ccgctgatga aatgggcttt gaaacaagac ctgttcaagc agataaaact ctctttgaca
     3721 tgagtgatgt cccctatcca tttatcgttc acgttaacaa agaagaaaaa ctccaacatt
     3781 actatgttgt ctatcaaaca aagaaagact atctggttat tggtgatcct gacccttctg
     3841 taaaaatcac caaaatgtca aaagaacgct ttttctctga atggactgga gtagctattt
     3901 ttctagctcc caaacccagc tatcaacccc aaccaaactc caatctgcga aggtcgctaa
     3961 taaccgtttg aacgaagtct atctagtcga atctgaattt caagttcaag aaaaccctgt
     4021 tcattcacat tttttgatgg gcgatattga atttgatgac ctttcttata agtatggttt
     4081 tggacgagat accttaacag atattaatct cacgattaaa caaggagata aggttagcct
     4141 agttggagtt agtggttctg gtaaaacaac tttagccaaa atgattgtca atttctttga
     4201 accctacaaa gggcatattt ccatcaatca tcaggatatt aaaaacattg ataaaaaagt
     4261 cttgcgccgt catattaatt acctacccca acaagcctat atctttaatg gctctatctt
     4321 ggaaaattta accttgggcg gtaatcatat gattagccaa gaagatattc taagagcttg
     4381 tgaattagct gaaatccgtc aagacattga aagaatgcct atgggctatc aaactcagct
     4441 ctctgatgga gctggtctat caggaggaca gaagcaacga atagccctcg ctcgtgctct
     4501 tttaactaaa tctcctgttt taatactaga cgaagctact agcggtcttg atgtcttgac
     4561 tgagaaaaaa gttatagata atcttatgtc cctaactgat aaaaccattc tctttgtagc
     4621 ccatcgtctc agtatagccg aacgaactaa ccgtgtcatt gttcttgacc aggggaaaat
     4681 cattgaagtt ggtagtcacc aagagttaat gcaggcgcaa ggcttctacc atcatctgtt
     4741 caataaataa ggagaatgtc atgaatccta atctttttag aagcgtcgag ttttatcaga
     4801 gacgttacca taactatgcg acagtattaa tcatacctct ttcattatta tttactttca
     4861 tcttgatttt ctcccttgtt gccacaaaag aaattactgt tacttcccaa ggggaaatca
     4921 cccctacaag tgtcattgca tctattcagt cgaccagtga taatcctatc cttgctaatc
     4981 atttagtggc aaatcaagta gttgagaaag gtgacttact catcaaatac tctgaaacaa
     5041 tggaagagag tcagaaaact gccttagaaa ctcaattaca aagacttgag aagcaaaaag
     5101 aaggacttgg aatcttgaaa caaagcttag aaaaagcgac tgatcttttt tctagcgagg
     5161 atgagtttgg ctaccataat acttttatga attttactaa acaatcccat gatattgaac
     5221 tgggtatctc aaagactaac actgaagttt caaatcaagc taatcttacc aatagcagtt
     5281 catcagccat cgaacaagaa attacaaaag ttcaacaaca aattggagaa tatcaagagc
     5341 tgagagatac tatcgtaaat aaaagagctc gcttaccaac tggcaatccg caccagtcaa
     5401 ttttaaatcg ttatcttata gcctctcaag aacaaacaca aggaacggca gaggagccat
     5461 ttttatctca aattaatcaa agtattgcag gtctggaatc atctatcgca agcctcaaaa
     5521 ttcagcaagc tggtatcgga agtgtagcaa cttatgataa tagtttagca accaaaattg
     5581 aagtactccg cactcagttt ttacaaacag cctcacagca acaactaacc gtggagaatc
     5641 aattaacaga attaaaagta caactagatc aagcaacgca gcgcttagga aacaatacct
     5701 taaccgctcc aagtaaaggt atcgttcatc tgaacagcga atttgaaggt aaaaatagaa
     5761 ttccaactgg tacagaaatt gctcaaatat tccctgtcat cacagataca agagaagtac
     5821 taatcactta ctacgtatct tctgactatc tacctctact agataaagga caaactgtaa
     5881 gattaaaact ggagaagatt ggaaatcacg gcaccaccat catcggccaa cttcagacaa
     5941 ttgatcaaac tcctaccaga acagagcaag gtaatctctt taaattaacc gctcttgcaa
     6001 aactatctaa tgaggatagt aaactcatcc aatatggctt acaaggtcgc gtcactagtg
     6061 taactgcaaa gaaaacatat tttgattatt tcaaagataa aattttaacc cattctgatt
     6121 aattttcaga taacactcta taactattta ttatcttatc aaaaaggaga atcataacat
     6181 ggataagaaa caaaacctaa cttcatttca agaactaaca actaccgaac tcaaccaaat
     6241 tacaggtgga ggactgtggg aagatatctt atacagcctt aatataatta agcataacaa
     6301 tacgaaagga cttcatcatc caataca
//
LOCUS       DAVAAL010000105         6090 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVAAL010000105 DAVAAL010000000
VERSION     DAVAAL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.106"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..135
                     /locus_tag="VQM60_001633"
     CDS             <1..135
                     /locus_tag="VQM60_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HET1081485.1"
                     /translation="AGSRHGDNRFYFPKEMLDQNSIFASITQQFLYLIENDHFTPNKN
                     "
     gene            148..954
                     /gene="yidA"
                     /locus_tag="VQM60_001634"
     CDS             148..954
                     /gene="yidA"
                     /locus_tag="VQM60_001634"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET1081486.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKSNDESGVAHAIRTWVL"
     gene            997..1995
                     /locus_tag="VQM60_001635"
     CDS             997..1995
                     /locus_tag="VQM60_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET1081487.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNKLECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLKVVELKTILSKHN
                     LPTSGKKAELITRIINNINVDKLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDITMED
                     YCKEKNNISFKATFGDIKWSILNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHS
                     LIYYIESLIITSSGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPCSTAEEINNYLKKYECYSPLNNLELDDVE"
     gene            complement(2167..2238)
                     /locus_tag="VQM60_001636"
     tRNA            complement(2167..2238)
                     /locus_tag="VQM60_001636"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2203..2205),aa:Arg,seq:cct)
     gene            complement(2280..2627)
                     /gene="rplS"
                     /locus_tag="VQM60_001637"
     CDS             complement(2280..2627)
                     /gene="rplS"
                     /locus_tag="VQM60_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET1081488.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(2746..3075)
                     /gene="crcB"
                     /locus_tag="VQM60_001638"
     CDS             complement(2746..3075)
                     /gene="crcB"
                     /locus_tag="VQM60_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET1081489.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(3069..3443)
                     /gene="crcB"
                     /locus_tag="VQM60_001639"
     CDS             complement(3069..3443)
                     /gene="crcB"
                     /locus_tag="VQM60_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET1081490.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(3440..3706)
                     /locus_tag="VQM60_001640"
     CDS             complement(3440..3706)
                     /locus_tag="VQM60_001640"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET1081491.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(3822..4265)
                     /locus_tag="VQM60_001641"
     CDS             complement(3822..4265)
                     /locus_tag="VQM60_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET1081492.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            4369..5304
                     /locus_tag="VQM60_001642"
     CDS             4369..5304
                     /locus_tag="VQM60_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET1081493.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            5358..5642
                     /locus_tag="VQM60_001643"
     CDS             5358..5642
                     /locus_tag="VQM60_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET1081494.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(5699..5917)
                     /locus_tag="VQM60_001644"
     CDS             complement(5699..5917)
                     /locus_tag="VQM60_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081495.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(5914..>6090)
                     /locus_tag="VQM60_001645"
     CDS             complement(5914..>6090)
                     /locus_tag="VQM60_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET1081496.1"
                     /translation="NPIEQVWKEIRKRGFKNKAFQTLEDVMNQLQDVIQGLEKEVIKS
                     IVNRRWTRMLCESR"
BASE COUNT         2003 a         1272 c         1044 g         1771 t
ORIGIN      
        1 gctggtagtc gtcacggaga taatcgtttc tattttccga aagaaatgtt agatcaaaac
       61 agtatctttg ctagcattac ccagcaattt ttatacttga ttgagaacga tcattttacc
      121 ccaaataaaa actagaagag gaaatttatg agtattaaac taattgccgt tgatatcgac
      181 ggaacccttg tcaacagcca aaaggaaatc actcctgaag tcttttctgc catccaagat
      241 gccaaagaag ctggtgtcaa agtcgtgatt gcaactggcc gccccatcgc aggtgttgcc
      301 aaacttctag acgacttgca gttgagagac caaggtgact atgtggtgac cttcaatggt
      361 gcccttgtcc aagaaactgc tactggccat gagattatca gcgaatcctt gacctatgag
      421 gattatctgg atatggaatt cctcagtcgc aagctcggtg tccacatgca tgccattacc
      481 aaggacggta tctatactgc aaatcgcaat atcggaacat acactgtaca cgaatcaacc
      541 ctcgtcagca tgcctatctt ctaccgtacc cctgaagaaa tggctggcaa agaaattgtt
      601 aaatgtatgt ttatcgatga accagaaatt ctcgatgctg cgattgaaaa aataccagcc
      661 gaattttacg agcactactc tatcaacaaa tctgctcctt tctacctcga actccttaaa
      721 aagaatgtag acaagggttc agctattact cacttagctg aaaaactagg attaaccaaa
      781 gatgaaacca tggctatcgg agacgaagaa aatgaccgtg ccatgctgga agtcgttggc
      841 aaccctgttg tcatggaaaa tggaaatcca gaaatcaaaa aaatcgccaa atacatcact
      901 aaatctaatg atgaatctgg cgttgcccat gccatccgta catgggtact gtaaaagtat
      961 catttttcaa taagaattga ttagcaataa aatccaatga atttttttag caaactattt
     1021 aatttaaaac aaaataatca taatagagac acaaattctg attgtaacaa tttttaccta
     1081 aacaaattag agtgcggcct tactcctggg caactcatac tcatagattg gactcaaaaa
     1141 acagggagaa attataattt cccaagatat tttaaatact ctcttcaaat tgaccctgaa
     1201 tctacacaca atcaattata caaattagga tacttcacta aaaataagac tttatcatat
     1261 cttaaagtag tagaattaaa aactatatta tctaaacata atttacctac ttctggaaaa
     1321 aaagcagaat taattacaag aataattaat aatatcaacg ttgacaaatt agatattccg
     1381 ttcgaattta aactaacaaa agaagcacaa aatcttatta tcgaacatag tgactatatc
     1441 aaagcatact atgataaaga cataactatg gaagattatt gtaaagaaaa aaataatatc
     1501 tcttttaaag caacttttgg tgatataaaa tggagtattt taaataaaca agctcatagg
     1561 aatactgtat caggagattt tggatgttta tctaacacac gaaaggctca aggaagacat
     1621 ttggaacaag aaggtaatat taaacattct ttaatatatt acatagaatc tttgataatt
     1681 actagttcag gattagaaaa caatttttca gccactgatt atccagtata ttatcccgat
     1741 tcgatacctg actactcact aaaacatatt caaacattaa tggaatcatt atctgatgac
     1801 gattatgatt ttgcttttga tgaagcatta tttcgcttct caattttgaa tgcaaatcat
     1861 tttttatcta aagaagatat tgactattta agagttaatt taccttgttc tactgctgaa
     1921 gaaataaaca attacttaaa gaaatatgaa tgttatagtc ctttaaataa tttagaactt
     1981 gacgatgttg aataaattaa ttatacaaac atttatatac tcgatatagt ctcaatttta
     2041 tctgatgatt gcccaaattt ttcaataata aaacgcataa tattatggag acacacaaaa
     2101 tcacctatat tatgcgttct tttaatatca aagacttttt gacaaacttc tttgatgtct
     2161 aattactgcc ccctgcagga atcgaacctg caactactcc ttaggaggga gttgttatat
     2221 ccattgaact aagggagcta gataaaaact ctgctgaaaa gcagagtttt ttagtcgaat
     2281 taacgacgaa tttctttgat acgagctgct ttaccttgaa gagcacgcaa gtagtacaat
     2341 ttcgcacgac gtactttacc gtaacgaaca acttcgattt tttcaacacg tggagtgtgg
     2401 attgggaaga tacgctcaac acctacaccg ttagagattt tacgaactgt gtagttttct
     2461 gagattccag cacctttacg tgcgataaca acaccttcaa aaatctggat acgttcacgg
     2521 ttaccttcga caactttcgc atgtacacga acagtgtcac caggacggaa tgatgggata
     2581 tctgtacgaa gttgaccttc agtcaagctt tggattaatg gattcatttt attctcctat
     2641 ctttgtcaat cttgaggaat cttcctcagc ggataaactg tatttttgtg cgtccattac
     2701 acacaagata tagtttacca aatttctaaa aaaaagtaaa gaaatttata gcaaaattcc
     2761 taaaaaaatc gcaaccaaac cacctatgta agtcaaagtc aggtaagaat aaaagacctt
     2821 cttatcactt agcagtcttt gaagttcgtc attcaaggtc gaaaaagttg ttaaaccgcc
     2881 acaaaatcct gttgctagaa tagcatatac ttccttagac tctacatgat tgtagaatac
     2941 tccaattaaa aaacaaccta aaagattagc tatgagcgtt ccgagtggca atttagaagc
     3001 ttgattatag cgagaaaaga aataccgtac gagtgctccc aagccacagg cgattgcaag
     3061 atagacgatt accatttctt cctccccaaa tagtaagcta aaagcaggcc tccaccgata
     3121 ctcaaaagca aatacagtat caaactaaga taacgccctg tatcaagcag cttcacagta
     3181 tcaagcatta ggctggaaaa agttgttaaa cctccgcaaa aacccgtccc cagtgctaaa
     3241 atcaagccct tactagtccc cttatagacc agatagccct tgacaagata aatcaagcag
     3301 aaaattccca gatagttgac aaagagagtg ccccaaggaa agtctggact ggctggtaac
     3361 caggtggaaa ctagatatcg gacaagtccg cccaacatag cagctagaaa aattcctagc
     3421 ggataaaatt gttctttttt catttgatat tttgatcctg ataatcacgc gaacgtttga
     3481 gtatgtccga aaaagtcgct acaatagtct cctgatagcg cttgtcctct acacgactcc
     3541 taaccttttc aaaaataact tcttctctct tagtatctaa aatcggttta cctgaagctt
     3601 tcttataagc aaccaccccc tcaactaaat gcattcgttc ttctaacagt ttgacaattt
     3661 gatcgtcgat ttgatcgatt tcttgacgaa taatatctaa atccatacag tctcctcctt
     3721 tatttgaatt attgtatcaa aaagccatca attaggctag taaaatccca actctcgata
     3781 agaaaaatgc actgattgat tacatcagcg cacttttatg tttatcctac tttagcagcc
     3841 aattcttctg cgaattgttc caagcgttca atatcttctt cctcggcaga aagatcaact
     3901 ttaacacact ctgaaccttt ttctgctcct gttgacacaa agacacggtc aaagtcatca
     3961 acagccttac agaattcatc gtagaaggta tctcctgagc cgaccactcc gtagattttg
     4021 ccattcaagt tgagatctgc taggtcttcg tagaagtcca tcatctcatc tggcaattca
     4081 ccatcaccat aagtataggt cgcaacgata gcgatgtctg cttccaagaa gtctgaagcg
     4141 tcaacagttg tacattcatc aacatcaaca tccaagccca agtcacgtaa tttgtctgct
     4201 acaatatctg caatttcttc ggtattaccg gtcatactgg caaatacaat ttttgctaat
     4261 gccatatcgt cctcctcaat ttatctttct ccattatatc acatcttttt cattttaaaa
     4321 ataaaaattc ttgtgctaca atgaaatgag aaagaattga ggttatttat ggagatttgc
     4381 caacaaattt tagaaaaaat taaagaatac gacaccatta tcattcatcg tcatatgaaa
     4441 ccagaccctg atgccttggg aagtcaggtg ggattgaaag ccttgctgga acatcatttc
     4501 ccagaaaaaa ccatcaaagc cgtcggtttt gatgaaccaa ctcttacttg gatggctgag
     4561 atggatcttg ttgaagatag agcctaccaa ggcgcacttg tcatcgtctg tgatacagct
     4621 aatactgctc gtatcgatga taagcgctat agtcaaggtg attttctcat taagattgac
     4681 caccatccaa atgatgatgt atacggtgac ctgtcttggg tcgatactag ttcaagtagc
     4741 gctagcgaga tgattaccct atttgcccaa acaacccaac tagccttggc agatcgcgat
     4801 gctgagttgc tctttgcagg aattgtcggt gatacaggtc gcttcctcta cccttctacc
     4861 actgcacgga ctcttcgcct ggctgcttat ttgagagaac ataactttga ctttgcggct
     4921 ctcactcgca aaatggacac tatgagctac aaaattgcta aactgcaagg ctacatctac
     4981 gaccatctgg aagtggatga aaatggtgct gctcgcgtta tcctgagtca agaagtcttg
     5041 aaacaataca atgtaaccga tgctgaaact gcagccattg tcggtgcacc tggacgcatt
     5101 gacagagtga gtctctgggg aatttttgtc gaacaggctg atggccacta ccgagttcgc
     5161 ttacgcagta aaatccatcc tatcaatgaa atcgccaagg agcatgatgg tggaggccac
     5221 cctctagcaa gtggtgctaa ttcctatagc ctagaagaaa acgaaatcat ctaccaaaag
     5281 ttaaaaaact tgcttaaaaa ctgataaaat acttgccaaa cttttcagaa tctgatagac
     5341 tagtacggta acaatctatg gctcgcaaag agaccatggc agaaaggaaa tattgcaaaa
     5401 tgaaaaaaga tatccatcca gaatatcgcc cagttgtctt catggacaca actactggtt
     5461 accaattcct tagcggttca acaaaacgct ctaacgaaac agttgagttc gaaggcgaaa
     5521 cttacccatt gatccgtgtg gaaatttcat cagactcaca cccattctac actggacgtc
     5581 aaaagttcac tcaagcagat ggacgcgtgg atcgtttcaa caaaaaatac ggtctcaaat
     5641 aatgataaga gaacagtttc ggctgttctt ttttgtttct tgaaatcaac tgctgttttc
     5701 atgttccaga ctcatctgta ggttcggttt ccatgctact aggcaggaag gaaatagctg
     5761 tttcaacacg tccataatga gctgtactat tgtcacgaac cacactttca ttgatggtcc
     5821 aagtggaatt cattttctta aaagcttctc ggactttttc caaatctttg gaggcaatgg
     5881 cctgctctaa ggtttcaaaa cgaggactta tactcatctg ctttcacaaa gcattctagt
     5941 ccatctccga ttaacgatgg actttatcac ctccttctcc agtccttgta tgacatcttg
     6001 aagttgattc atgacatctt ccaaagtttg aaaggcttta ttcttaaatc cacgtttacg
     6061 aatctctttc cacacttgtt caatggggtt
//
LOCUS       DAVAAL010000106         6066 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVAAL010000106 DAVAAL010000000
VERSION     DAVAAL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.107"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..683)
                     /locus_tag="VQM60_001646"
     CDS             complement(<1..683)
                     /locus_tag="VQM60_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET1081497.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSV"
     gene            complement(1368..3002)
                     /locus_tag="VQM60_001647"
     CDS             complement(1368..3002)
                     /locus_tag="VQM60_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081498.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(2983..3732)
                     /locus_tag="VQM60_001648"
     CDS             complement(2983..3732)
                     /locus_tag="VQM60_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081499.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            3911..5365
                     /locus_tag="VQM60_001649"
     CDS             3911..5365
                     /locus_tag="VQM60_001649"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET1081500.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(5455..>6066)
                     /gene="alaS"
                     /locus_tag="VQM60_001650"
     CDS             complement(5455..>6066)
                     /gene="alaS"
                     /locus_tag="VQM60_001650"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Catalyzes a two-step reaction, first charging an
                     alanyl molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET1081501.1"
                     /translation="GGTHVGNTSEIGLFKIVKEEGIGSGTRRILAVTGKEAFEAYREQ
                     EDALKAIAATLKAPQVKEVPHKVEGLQEQLRQLQKENAELKEKAAAAAAGDIFKDVKE
                     VNGHRYIASQVSVSDAGALRTFADNWKQKDYSDLLVLVAAIGDKVNVLVASKTKDLHA
                     GNLVKELAPIIDGRGGGKPDMAMAGGSNQAKIQELLDAVAGKL"
BASE COUNT         1957 a         1312 c         1156 g         1641 t
ORIGIN      
        1 actgaaaaag ccgctgtgta ggaaaacata ttgagcaagg atttacccat agccaagccg
       61 tcaactaaac taccgcgaac ctcatgctgg tctaggaaaa ttcctgtcat caagccatca
      121 ttcataaaga cttgatacag gacaccattt tctaaaacag tgaaaaagtc aggtgcttct
      181 tgaccataaa catgggcaga ttcatagtca aaacccttaa agcggatttt ctcataagcc
      241 cctaaaacct cagggaaaac ctgtctaaag gcttctgata tagtctgacg aatctgataa
      301 acataagagt tataccaaga aaagacggcg tagtcgccat aaaggtccac tgtcagaccc
      361 ccaaagccat ctccctcttg attaaagaga cgaaaggcag ttgtcaaatc atcttgatag
      421 taggcgtttc tcttttcttt ggcttttcta aacaacgttt caaagaaagt ttgattgaag
      481 gccaccttgt ctttgctgac aaaccagccc aagcccttgt tttgctgaga aaggtaggca
      541 gtcccaagaa agtttccttc ctgaccctgc acctctactt cctgatcctt aagattgaca
      601 ttctcaagat cactggcttc tagtaaaact agccccttag caagcttctt ttcaaccctt
      661 ttgctgactc ttattctatt cataactacc attatatcaa acttttagac aattctcaaa
      721 aaagaaacta cccttgcttt tttactcttc ttttaaaaaa tggtatacta gacttcctgc
      781 aaaactagga aataaatgtg taagaatcac aataaaaaat gctcttccgt cttggaggag
      841 catttctttt tatcaacgaa aatcaaatag caaactatga aactagcctc aggttaaccg
      901 tgagattata ggtagagagg ttgtatcagc aatatgtgtc tgtcaaattt agtgacaaag
      961 gtagtagaag aaagatgaag aaataaatca gcttcagtag gtatctggaa aatttgattt
     1021 tatagagaag ccttttgtta caaactcaat atactatcaa taaataatat tatagaagca
     1081 acaataatta aaatttcacc tatttgcatc attctatttc gaactctaaa tatatgttct
     1141 atcaaaaata cttggatcac acacattata ggaattaacg tttttgaaat tgaaaaatat
     1201 cccaataaat aaactataaa caacaataat agaactatat tatatttctt attcaaaaca
     1261 ttcctcccta tatttttgat taccaatctt aatcatttac aactacattc taacaaacta
     1321 taaaagcgtt tgtcgaattg aatttatcaa gcaagcaacc aactatctca tcttttttct
     1381 atttctgcca atatgcgtga caggtaataa tgatagccaa aaatagcaag agcaagcaag
     1441 acgataagag ctcctactcc caagttgatg gcaaggatag gggagagaga ctgaaccaag
     1501 aatatgctcc caattacaag ggccatcagg attgcactat aaataaacaa taaaactatg
     1561 gcgactatgc catttgaacg attcaccagg tccgtaatgc tactccaatt ggttgacaga
     1621 tttttaacgt ccttaaagta atggtggcaa gaaaggatga cactggcaat gatccagact
     1681 acaagaaggt aaatcatcga aaggatggac aagcctagat atagagaaag accgagcaaa
     1741 gtcagaactg gtaaaaagga ctgaacagca aagataatcc aaaatttcac tttcacataa
     1801 cgagcaaagt caaagggtaa actcttcaga aaatcaacat tttccctctc caaggacaag
     1861 gcaattgaat gcaggctggt gatattgtta ttgacaactg ctataaacac tcctacaaaa
     1921 aacaagggta accagtatgg aggatgaatg tctggaacta tctgagaatc tcggattttg
     1981 gaaatcagac tgatcatcat gagataagga aggaaagcac ttgtaaaaag cactgtaatc
     2041 acaccagtcc cctgtcccaa gagggtgagg tggtagcgta aaaccatacg gaaaaagccc
     2101 tttttagtag ttgaaatcct ctccttgctg cgacgttctt ttttgacctt ctcctcactg
     2161 ttaagcagga tcacgtcata aaaacgagga aggaccttct ttttggtcag ataaagcagg
     2221 aagagagtta gtcctatcca agcgagcaga cccactaagg cttctgtcga aaaaggctcc
     2281 actgctattt tgtaaaagat atgaagagga taaaggagaa atggaatgtc tctaactttg
     2341 tcaacaatac ttccaaaagt cgactgaaga aagaagataa atattaaagg tatgagaact
     2401 cctatcccaa tcatcacatt cgaaaaaata gactgatact ttctgaagac cctagtctga
     2461 gccaagaaat gtactgccac taccgtcact aaagtaacag agacaaataa taaggtcaag
     2521 gacagtagca tcaaaggcaa acccagccaa agagaaggag ctagactaat atagagggct
     2581 agaaaataag ctaggattgg tacaattcca gttagagctg gcaagaggac agacagacct
     2641 ttggccatta taatctctga ttctttaaag gcatagggcc tataggaggc caggtcctta
     2701 ctctcataaa agacattgta aaaggcagtg aaagaagttg aaaaggccag caccagtaaa
     2761 atagcaacca tcgagctaaa ataaataggc atttcctcaa aaggaaaatg aatggctata
     2821 ttactaaaac agatgagcat caagagactg gaaaaaatgt aagaacttag gactctagca
     2881 gaaacattta cttttttccc aggattctta gcctgcttct ttcgtagatt agccagatta
     2941 gcttcttgag atgaatagag gatattgata tcaactaatt ttttaatgac cttgagacgc
     3001 atccgcaacc tcctcttttc taccagcaag actaaggtag atactttcca aaaactggtc
     3061 tgggtggtct ttcctcaagt tctctacact accacaatag atcaaatgcc cctttttcaa
     3121 aatagcaatc cgatcacaga cttgctcggc tacctcaagg acatgagttg aaaacaagac
     3181 cgtcttgcct ttttgtgcat gttccttcat catttgcttc aaatcaaagg cggcctgggg
     3241 atccaaacca gtcaagggtt cgtccaaaac ccaaatatca ggagcagaca agagtgcccc
     3301 aatgacaaag actttctgac gcattccgtg agaaagagtt tcaataacct gatagcggtt
     3361 ttcagcaaaa tcaaaaacgt tcaatagcct agctagacta gtctccaagt cagagctagt
     3421 cagatcatag gatgaagcga tcaattccca aaattcattg gccgttaagc gtaaaaataa
     3481 gtcaggcgag tctgctacgt agccaatctt tcgtttgatc gccaagcgat tttccgataa
     3541 ctcctgaccg tctaccaaaa tacgaccact gctgggtgaa atgatactga ctagggattt
     3601 tatggtggtc gattttccag ccccattatg accaatcaag cccataatct ctccattttc
     3661 aatctgcaaa ttgagattac tcaaggcctc tttatcacca tacaacttac taacattttg
     3721 aaattcaatc atgggacaac tcctatcttt atctgtatta tacatcgcta tactagcact
     3781 ttgatacaaa aaaatcaata ctttatttta agtagttaaa atagtttcct tctatcttac
     3841 gcaaacgttt gcgccgagag atactttatg atagaataaa gaacaagatt gacaagtaag
     3901 aggaaacatc atgcaaaatc aaacactcat gcaatacttt gaatggtatc tgccccacga
     3961 cggtcaacac tggacgcgtc tggctgaaaa tgctccacac ctagctcatc tggggatcag
     4021 tcacgtctgg atgccaccag ccttcaaggc aaccaacgaa aaagatgtcg gctatggggt
     4081 ctatgactta tttgacttag gagagttcaa ccaaaaaggg actgtccgca ccaagtatgg
     4141 tttcaaagaa gactatcttc aagccattca agcccttaaa gcacagggaa ttcaacctat
     4201 ggccgatgta gttctcaacc acaaggctgc tgccgatcac agggaagcct ttcaggtcat
     4261 cgaagttgat cctgtagacc gtacagttga acttggagaa cccttcacca tcaatggctg
     4321 gactagtttt accttcgatg gtcgccaaga tacctataat ggcttccact ggcattggta
     4381 ccacttcact ggtacagact acgatgccaa acgcagtaaa tctgggattt atctgatcca
     4441 aggggacaac aagggctggg ctaacgagga attggtcgat aacgaaaacg gaaactacga
     4501 ctacctcatg tatgccgacc tagactttaa acatcctgaa gtcatccaaa acatctatga
     4561 ctgggctgat tggttcatgg aaacgactgg tgtagctggt ttccgtttgg atgccgttaa
     4621 gcatattgac tctttcttta tgcgcaactt catccgcgat atgaaggaaa aatacggtga
     4681 cgatttctat gtttttggtg aattttggaa cccagacaag gaagccaatc tggactatct
     4741 cgaaaaaacg gaagaacact ttgaccttgt cgatgttcgt ctccaccaga atctctttga
     4801 agccagtcaa gctggcgcaa actatgacct tcgtggcatt ttcacagata gcctggttga
     4861 actcaagcct gacaaggctg tgacttttgt cgacaaccac gatacccaac gaggacaagc
     4921 ccttgagtct accgttgaag aatggttcaa gccagcagcc tatgccctca ttttgttacg
     4981 ccaagacggc cttccatgtg tcttttacgg agactactat gggatttcag gccagtatgc
     5041 tcaagaagat ttcaaagaaa tccttgaccg cctcctagcc atccgaaaag atttggccta
     5101 tggagaacaa aatgactact ttgaccatgc taactgtatc ggttgggtac gttcaggtgc
     5161 tgaaaatcaa tccccaatcg cagtccttat ctcaaatgac caagaaaaca gcaagtcaat
     5221 gtttgtcggt caagaatgga ctaatcaaac ctttgtagat ttacttggta accaccaagg
     5281 tcaagttaca attgatgagg aaggttatgg acaattccct gtctcagcta gatccgtaag
     5341 tgtctgggca gtcaatacca tctaatagct cataataacc aagctaggtc caagcggatt
     5401 tggctttttt gtattcacaa aaagacctac ccaaatggat agatctttac ttgattacaa
     5461 tttacctgct actgcatcca acaattcttg gatcttagct tggttgcttc ctcctgccat
     5521 ggccatgtct ggtttaccac caccacgtcc atcgatgatt ggtgctaatt ctttgacaag
     5581 gtttcctgca tgaaggtctt ttgtcttgct tgctacaagg acattgactt tgtcaccgat
     5641 agcggcaact aggacaagaa gatcagagta gtctttttgt ttccagttat ctgcaaaggt
     5701 acgaagggca cctgcatcgg atacagacac ttgactagca atgtaacgat gaccgttgac
     5761 ttccttaaca tctttgaaga tatcgcctgc ggctgcagct gcggcttttt ctttcaactc
     5821 agcattttct ttttgaagtt gacgaagttg ttcttgaagt ccttctacct tgtgaggtac
     5881 ttccttgact tgaggtgctt tcaaggttgc tgcgatagct ttaagagcat cctcttgttc
     5941 acgataggct tcaaaggctt ccttaccagt cactgccaag atacggcgag ttcctgaacc
     6001 gattccttct tctttgacaa ttttgaagag accaatctca gaagtgttgc caacatgagt
     6061 accacc
//
LOCUS       DAVAAL010000107         6062 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVAAL010000107 DAVAAL010000000
VERSION     DAVAAL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.108"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(98..1324)
                     /locus_tag="VQM60_001651"
     CDS             complement(98..1324)
                     /locus_tag="VQM60_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET1081502.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(1396..2061)
                     /locus_tag="VQM60_001652"
     CDS             complement(1396..2061)
                     /locus_tag="VQM60_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET1081503.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFVVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(2129..2326)
                     /locus_tag="VQM60_001653"
     CDS             complement(2129..2326)
                     /locus_tag="VQM60_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HET1081504.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            complement(2617..3480)
                     /locus_tag="VQM60_001654"
     CDS             complement(2617..3480)
                     /locus_tag="VQM60_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET1081505.1"
                     /translation="MIERMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTSLFE
                     LRFQQYKELID"
     gene            complement(3875..5046)
                     /locus_tag="VQM60_001655"
                     /pseudo
     CDS             complement(3875..5046)
                     /locus_tag="VQM60_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            complement(5059..5798)
                     /locus_tag="VQM60_001656"
                     /pseudo
     CDS             complement(5059..5798)
                     /locus_tag="VQM60_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
     gene            complement(5810..>6062)
                     /locus_tag="VQM60_001657"
     CDS             complement(5810..>6062)
                     /locus_tag="VQM60_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081506.1"
                     /translation="LYIFRYNSCMKIFNFRDKGLIFFNKIVNGLIQNIEENLEDDIER
                     ILKYLYICLFNEIFIIKNKVNFFDDVEFNQTLSEFLDKL"
BASE COUNT         2108 a         1146 c          923 g         1885 t
ORIGIN      
        1 tataacacat tcagaaaaga gaaaaaagtc tgttgatttt gaccatcata aaaagactgg
       61 caatccagtc tcaaacatat attatagaaa ttctccacta aatactttca cgaatattca
      121 gaagcataac aaaggcaact agaagaaata gcaataaaac aaagctaact gccagagttc
      181 caaagctagt agcaatggtt accaaagcta ttgtaaataa gctaggtaaa acaaccgtaa
      241 tggcaccgat agaggattga actgctccca ttgactcctc aggtatttgt ttaaagacga
      301 gttcttgcaa tctaggagag agaacacctg cgaaaaaggc atccaaggta ctaaagatga
      361 gaatccagtc aaaacgaact gtggcaaatc ctactagaag aagcaactgg atgacaagtg
      421 aggcatagag agctgttttt atggaaatgg tatgttgcag atagccactt acaaggcttc
      481 cgacaatcag ggctgacagt tcaaaagtgg atagcaaggc aagagattga cctgtttgta
      541 aattcaaaaa gggttgattc ctcaaaaata gagtagagac aggaactgta acatttatca
      601 ctgcttgact agtagagata ataaacaaaa ccaagagcac cttattcata ttccatatca
      661 atttcgatga ttggagcaaa tgctggcaaa aggattttac agagagtcct tcttgatagt
      721 taatcgtttt ttctactttc aagaggtcag tttttatgaa gaggatacct aaaaatgcga
      781 ttaaaaaggt aagagcgttc agcaaggaaa tgaactggat ggagagaatc cctagcaaga
      841 ctcctcctag aatattactg attgttttca ctaaactaac agttgactgt ttaaatccaa
      901 tagcttctgc cagatggtct tgcccaataa ttctaatgaa aatcggagtg agcatggctc
      961 ctgaaaagta actcaatgtg tcagacaaga ggttaatcag acaaataaag gttactagca
     1021 acaaggaaaa agactgccct gaaagcgata gggacactat agagtaaagc aaaaattttg
     1081 taaaactaat gactgtgtat ttcaagacac gatgatgttg aaaatccgcc aaaactccca
     1141 gaaagatttg tagaacttgg ggaagggttt ctgaaatcgt gataagtaaa atcgccaaag
     1201 gggcaaaaga cgcatctgcc acataattca ggaaggccag ataaaaaatc gtatccccaa
     1261 gcgttgaaat ccactggttg atagttaatt gcctaaaatc tctattttga agaaatactt
     1321 tcattacaac tcctttttaa attcaaatgg gaatctttcc ccaagaatag accgcgatac
     1381 tactaacaac caaaattaca gtaacatcaa aagctgacca atgccgttgt agactatatg
     1441 cagcccaata ggccaataaa tggactttgt cattctaaat aagactacaa atataagtcc
     1501 gccacccata tagaagacaa agtctgtcaa gacccaaccg tgattactaa tgtgcgagac
     1561 cccaaataaa atagcagaac caagtacatc tagcccccat ttctttcctt tttccagagc
     1621 tgtcatcacc aatccccgat ggattatctc ttcaaaaatg ggacctgcaa tcacaggata
     1681 gaaaaaatac atcaaaaatg ctgtagcccc tgtaaaagtc ggagcagcat gttgataaga
     1741 aatttcattt cgagtaggtg ggaaaagaaa aaaggtaacg aaattccaaa caacaaaagc
     1801 aagcagagct aggaaggaat agaaaagata ggatccttta aactttctac tattgatttt
     1861 ctgccatttc cccgaccaaa tcatagcaat aagagcaaat aaaaccacaa gaaaattcaa
     1921 catcatatcc gacagataat aggcaaagtc agatagccca gtaacaaggt cgctgcgtaa
     1981 aactagaaca ctgaacttct ggtcagcaat aactagtaga aaaactataa taaagtagcg
     2041 gtgtgagatt atctttttca ttctattttc tcctatacta tgaaataaag acagtcccta
     2101 cccaagaaaa aggcaaggac tctgaatatc aataatggcg acgattaaca gtggaagaat
     2161 catggttaaa atatttcttc cagaaattag tgattgacca agacgttcct aaacctcccc
     2221 aagcaccggc tgggataaca gcattattcc caaattcccc tccataaact gtctccaact
     2281 cagcttctgt caattccatt gtttctgcaa attgtaaatt taacatcttt tacactcctt
     2341 caattatctt catttgtaaa ccacttctgc gacctaggat ttgcttcaag tgctttacaa
     2401 gtacagtata acacgaaaat tggcttattt taaaaaatcg catatttgat attttttctt
     2461 atagaaattt cttatttgcg attttgatag atttgattat ttccctggta taataaagtt
     2521 attactaaca aggaggaata taatagatga agaaaagaaa aatccaaccg atactcctac
     2581 ttatttcgga gtgggttatt gtcattccat ttttaactaa tctataagtt ctttatattg
     2641 ctgaaaacgc aattcaaaaa gggatgttaa ttgtggattt tctaatacct gcagagattg
     2701 gataaagcgt tcaatctctt tttgattgct tccctttgtt tgaagaaaga cactcatctt
     2761 ctttaaaaat tgccacgata ctttttcaaa aacatcatac ggtcgtaaca tcctctccaa
     2821 ctcggcttcg aagattggga tgtaggagaa aagttttcgc tccatgagtt ctgataagat
     2881 atttaagagt ccttgcttca tatacaatcg attgtgtact aactctttaa attctttgga
     2941 tttttcgagt aaggaggttg ataaaaaaat cagatcttga ttgctcaaga agggcatggt
     3001 attgcaaaag agatagagtt caaaccaggt ccaagactcg atagcataga gataggtggt
     3061 caaaaactcg ctatcctcct ctgctagtgg gtagctttta tttagtgaat ggatggcatc
     3121 tttaatcacg atggcattca aacgacgata ggtctgcgcc atctgttctt gatcgacttc
     3181 ctccaatagc tgctctaaag cagctatatc ctgatgggca aagcgattca caacctttcg
     3241 accgattcgc atatgtggag attcttgata gttgttgagc ttgtgcccaa actcatcaaa
     3301 ggtcacattt ataccttgga tagctagaat caacttatcc gcagacagca tagactgccc
     3361 tagttcaaac ttggacaact gagaagctgt tagaccctca caagccacat ctgtctgctt
     3421 aagctttctc gccaaacgta attccttgta aaattccccc agttccattc tctcaatcat
     3481 ctgaccacct cctagctttt gcatattata tcattattta caatcatttg tcaaaatatt
     3541 ctgatattta agagggcaaa aaagccagcc ttaagctgac tctaaaagga ccgagccttg
     3601 tacaatacag aactcaatcc tcgttctaaa tagaatgtct aacttttttg ggcagtacac
     3661 gagcttgggg aacagccact ggaacgatga ggtgtgagct caaaatatcc tccagttatg
     3721 tttttcctaa tagtataccg gaagagtgaa aggattttat aatggagcgg ttacaaagaa
     3781 cctactttct attaaacagt atactatgaa aatgtgaaaa tttaacattt ttttgtacaa
     3841 attttataaa ttattgcctt tttaatatca atagttaatc tcttatccag atcccccttg
     3901 tgtaaacttt atctttataa gcttcaaggc ccctatccca tctatttgca acaattagat
     3961 cactttgttt tgtaaatagt tcaaaattct tttcaataat tacgttatct atactaacgt
     4021 ttaaatttgg ttcatatact aaaattttta taccgacaat caatagttca ttaattatac
     4081 ttaaaatagc tgactctttg taattatctg aattatattt catccccaat ttatatattc
     4141 ctactatctt tggctttcgt tccaatattt gtttaactat gaactgtttt ctatttgtgt
     4201 ttgaaatatc aatcgcttct atcactgggg catttatttc tataaattct ttttttaatt
     4261 gtttagtatc tttgggaaga caatatcctc caaatccaaa agaaggatta ttataaaaat
     4321 ttccaattct tggatctaaa caaacacctt ttattacaac ttcagcattt aagcttctcc
     4381 tctcagcaaa agaatctagt tcattaaaaa agcaacacgg agagctaaga atgtgttaga
     4441 aaaaagctta attgcttctg cttcagtagg agaaactaac ataacatttt taatattggc
     4501 agtactatga gtactaatcg aaaggaacaa ctctgcaatt tttcttcctt caactgtctc
     4561 atctccaaca actatgcgac ttggatataa attatcatat atagaacaac cttctctcaa
     4621 aaattcaggg acaaaaatga tattttttgt atcaaacagc ctttttaatt tgtttgaaaa
     4681 gccgatcgga actgttgact ttaaaataat ctttccatta ggttttaccc tcagaatctt
     4741 cgataccgtt tgttcgattt catatgtatt aaaactacca attttctcat cataatctgt
     4801 cggaagcgca ataatataat aatcaatatt atttttaatt tcagaaaatg tatcaaaaaa
     4861 agtaatattt aagttattct cgcaaaaaaa cttcataagc tcttcatttt tagatggaag
     4921 aatgcccttt tttaaattat ttatttttac agaatctata tcatatgcaa caactttata
     4981 tttagatgca aatagtaacg cgtaggccag cccaacatgc cccaaaccaa ttactgctat
     5041 attcataaaa ctacttcctt atttcttaat ccaaaatata atagaataaa ctgccccatt
     5101 ccttaaatac aactctttaa tattgtttaa aagtttttca actgatttcc agattatcaa
     5161 aatctgagat ttatagcaca atattgatga tattctatca atataatgtt tttcatcgag
     5221 ttcctcttga tacattttta attctttagt ttttcccata taactaacca tactactatc
     5281 acttacatat gggaaatcct cataatatat tactttataa cgcataaatt caagcgccct
     5341 tccaatacta ttcacaaaaa catgatcaac atggtcacca agtgaaagcg gacaatatac
     5401 gacacatttg tcgtctaaat gcattaacag ctcttttatg atatcattct ttaatgtgtc
     5461 ctcatttttt aattcactat agatatgacg gtatagaaaa ttgccatttc tatctttcct
     5521 atagagacat tcatagtacg ataagtgtct aaaatcacat tgtagacgtt cacaagctaa
     5581 cctatcttct ttcttccttt cttcaatcgg atattacaca acttatgaaa ttgcttagca
     5641 gagggctgta gctgttgact caaagggtaa ccagaaaata tagtaataac aagtacaatt
     5701 tctccttctg aagttaattt tgaaatataa tcaccacagg aaaaaattgc gtcatctaaa
     5761 tgtggagata aaaagatata cttagtattg ttactcataa ccattccctc tacaatttat
     5821 ctaaaaactc actaagtgtc tgattaaatt ccacatcatc aaaaaaattc accttattct
     5881 taataatgaa tatttcgtta aataaacata tatataaata tttcaatatc ctttcaatat
     5941 catcctctaa attctcctca atattttgta tcagcccatt tacaatctta ttaaaaaaga
     6001 taagcccttt atctctaaaa ttaaatattt tcatacaact gttgtatcga aaaatatata
     6061 aa
//
LOCUS       DAVAAL010000108         6031 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVAAL010000108 DAVAAL010000000
VERSION     DAVAAL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.109"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            1..550
                     /locus_tag="VQM60_001658"
                     /pseudo
     CDS             1..550
                     /locus_tag="VQM60_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(590..1600)
                     /gene="tsaD"
                     /locus_tag="VQM60_001659"
     CDS             complement(590..1600)
                     /gene="tsaD"
                     /locus_tag="VQM60_001659"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET1081507.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKTFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(1590..2027)
                     /gene="rimI"
                     /locus_tag="VQM60_001660"
     CDS             complement(1590..2027)
                     /gene="rimI"
                     /locus_tag="VQM60_001660"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET1081508.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(2024..2707)
                     /gene="tsaB"
                     /locus_tag="VQM60_001661"
     CDS             complement(2024..2707)
                     /gene="tsaB"
                     /locus_tag="VQM60_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET1081509.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            2885..3070
                     /gene="cibA"
                     /locus_tag="VQM60_001662"
     CDS             2885..3070
                     /gene="cibA"
                     /locus_tag="VQM60_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET1081510.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            3072..3224
                     /gene="cibB"
                     /locus_tag="VQM60_001663"
     CDS             3072..3224
                     /gene="cibB"
                     /locus_tag="VQM60_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET1081511.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(3547..3762)
                     /locus_tag="VQM60_001664"
     CDS             complement(3547..3762)
                     /locus_tag="VQM60_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081512.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            4042..4275
                     /locus_tag="VQM60_001665"
     CDS             4042..4275
                     /locus_tag="VQM60_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET1081513.1"
                     /translation="MIYKVFYQETKERSPRRETTRTLYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            4277..5956
                     /locus_tag="VQM60_001666"
     CDS             4277..5956
                     /locus_tag="VQM60_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET1081514.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
BASE COUNT         1722 a         1415 c         1213 g         1681 t
ORIGIN      
        1 accaatttga ccgaaaatcc ttggagtatc gggcgctcaa gcgcttttgg aaccctcgct
       61 ttttcgtttc taggctcggg ctaaatcagt ccactggact gatttactac accagtatag
      121 cttcaagctc tgtcagaaac gattctatca gcccacgttt cgaatgcact taacccatcg
      181 ggaagtacga gataagctgc tttcttactc tgagggatta caggttcact acgaactcta
      241 tcaactcctg ctctttcatt ttcaagagaa gaatgccgac catttctttg gattgattga
      301 gcaagaactg ccaacggttc atccgctttt tcaaacggtc ttttggactt ttttaaggga
      361 tagagataag attatcaacg cacttaagct gccttattcc aacgctaaac ttgaagcgac
      421 caataatttg attaagatta tcaagcgcaa agcctttggt ttccggaact ttaacaattt
      481 taaaaaacgg attttgatga ctttgaacat caaaaaagag agtacgaatt tcgtactctc
      541 cagattgtag cttttcgcct acccactaca cttgacaaag agccactctt tattccatgg
      601 tatcaaaggc aagacttggt ttggcattga ggtcccagcc tgcgaagttt tctttgttcc
      661 actcgctgac gctggcatag gcaatcatac ctgcattgtc tccgcagagt cgcagagggg
      721 ggatgataac cttgacatct gtgatttcgg ctgctaggcg ttctctgaga cctttattgg
      781 ctgccacacc acctgccaca accagggttt taacaggata tttctccaaa gccttcttgg
      841 tttttgccat gagaatgtcc ataactgctg cttggaagga agcacacaaa tcttctgtag
      901 acaggctttc tcccttttgc tcggcattgt gataaagatt gataaaggca gatttcaaac
      961 ctgagaagga gaactccaga ttatcttcct taatcatggc acgggggaaa tcataaatat
     1021 cctgcccctg atgagccagc tcgtcaatct cacgacctgc aggataggtc aagcccatga
     1081 cacggccgac cttgtcataa gcctccccaa ctgcatcgtc tcgtgtctcc ccaacaatct
     1141 tataatctcc tgcctccgaa acatagacca actctgtgtg gccaccgctg accaagagtg
     1201 ccaacaaagg aaactccaaa ggctccacac tctgagcggc catgaggtgc ccagccatgt
     1261 gattaacagg gatcagcgga agtccgtgag cccaagcaaa ggtcttggca gctgacaagc
     1321 caactagcaa ggctccgact aagcccggtc cataggtaac cgcaacagct gtcacgtcct
     1381 cttcggtaat ccctgcttct gccaatgcct cctcgataca ggctgtaatg acctcgacat
     1441 ggtgacgact ggctacttcg ggcactacgc caccaaaacg tttgtgactc tcaatttgac
     1501 tagcaatgac attggacaag agctcatcgt cgtttttcaa gacggcgaca ctggtctcat
     1561 cacaggatgt ctcaaatgct aaaatatatc tatccttcat ctatttctct cttcatgata
     1621 atggcgtcct cgactgggtc atggtagtag gcctttcgct cagcgataac tgccatcttt
     1681 tctttcttgt aaaatgcttg cgctcgttga tttgactgtc tgacttcgag gaaaatttcc
     1741 ttgtctgtcg gcaattgagc aaacaaggct gacgcaattc cctgaccctg ataagctcct
     1801 ttgacagcga tttgcaggac ttctgcttca aaaagattct cctgcacagc tagaaatcca
     1861 atcacttctg ccccatcata agccaatgca taccaagtct ggtcttggga cagatctgct
     1921 tggatttgct ccagagtcca aggactgact aggtaaacag ctgccataac agcgtagatg
     1981 gcttgagcta ggtcaggctg ttgttgaatt cgcttgattt ctatcatagg cgtttaatgt
     2041 aagactcgcc agactcggta tggttcttga gccagttttc ctcagcctcg actcgtttga
     2101 ggtaattcgg cacaaaatca tgcaaggagt ctgcttcctt gtcccaggcc aaaagagcta
     2161 gattagctgc attgggcaat gtttctttgt aatcagtcct tggcaagtgt ttttgaatct
     2221 gctcaacaaa ggggccaact tctccgacaa aggttacctg actagtaccc ttgacttttt
     2281 ctagcacctc ttcaaaagat aggtgcgctt ctgccatgac aggtttggca ttttcataaa
     2341 atcctgcata aacattattg cgacgcgcat ccatcaaggg gacaaacaaa ccttcttgtt
     2401 ggtggggcac cagagccaag agactcgaca tcccaactaa ctcaatgttc agggtgtaag
     2461 ctaaggtctt agcagttgct accgcaattc gcaagcctgt atagctaccc ggcccttcag
     2521 ctaccacgat tcggtccaaa tccttgggtg tccaatccaa acttgccatc aaaaaatcga
     2581 tggcaggcat aagagtaata ctgtgatttt tcttaatatt aatcgtcgtc tcggcaagaa
     2641 cctgcttatc ctctaaaata gccagagaaa gagccttgct ggacgtatca aaagctaata
     2701 ctttcataac acattcctat ctttttgtct gcttactatt atactacaaa agctggcaca
     2761 tgggaatttt ctttgccccc agacaagagt gccctcactt aactaaaaat aatttaaaaa
     2821 aatgctcact tttccttttc ttttccgaat ataaaagtga acaagaaaaa aggaggaaag
     2881 ttcaatgaca aattttgaca ttcttgacaa tcaattttta tccttatctg aaaatgaatt
     2941 atcagatatt gatggcggtc tcgctccctt ggttatcttt ggagtagcag tatcttggaa
     3001 ggctattgca ggtggaacag cacttatagg ttctggtttg gcagctggtt attttttagg
     3061 aggagattaa tatgatgaaa gatttgaaca attatcgtga aatttctaat aaggaattgc
     3121 aagaaatcaa gggtggcttt ggtgtcggtg ttggtatcgc tttatttatg gcaggttata
     3181 ccattggaaa agaccttcgt aaaaagtttg gtaagtcatg ctagataaga aacacatttt
     3241 tagaaggata aattttattg tcttcatctc ttacagtttg ctcagcattc tcaatgattt
     3301 gaacattact accatccctt taccattcga tttatctgtt tgtattgttt tatttttatg
     3361 cttcaactct atttttgatc agaacaatga ctcccataaa aataataagc tttgaaaatt
     3421 ccattgtcat gtcatgttag aaaaatgcaa agaccacctc atcttgatag atggggtgga
     3481 attttcgtgt cgtaaatcta ctatctctac atccccaaac aaaaaacccc agcataagca
     3541 gggcatctaa gcatttaatt caaagtaaaa tacaaaccaa acgacatagg tcacgaggag
     3601 gagaaaaagc gagtagagag tcacaaaggt cattttccac aagaacttgg tttgtcgtcg
     3661 ttccagtttg gcaaatagaa gattccccgc ataaacgcaa gcaacaaaaa caataaaagc
     3721 taccaagcga gctccgatag caaaagcaaa taagttatac atagggcaac ctccttgact
     3781 taaaatctat atggaattat gacaagcaat aaatttcact tccgttatca acataataca
     3841 ttttctttat tcttgcaaac gcttaccaaa gaaatcgtcc cctaactttc tcgtttccgt
     3901 cttttactaa tttttcattt tgtggtataa ttgaaataat tgtaacgaat caaggtcaat
     3961 ctagacacaa aatggaatga aatcaagcaa atatctgcta aaagtttgga ataagctgac
     4021 ctgtaaatag aaaggaacta tatgatttac aaagtttttt atcaagaaac aaaagaacgt
     4081 agcccacgcc gtgaaacaac acgcacgctt tacctagaca tcgatgccag ctcagaactt
     4141 gagggccgta tcactgctcg ccaacttgtc gaagaaaatc gcccagagta caatatcgag
     4201 tatatcgaac tcttgtctga caaattgctc gattacgaaa aagaaactgg cgccttcgaa
     4261 attacggagt tctaatatgg cctacactct taaacctgaa gaagtcggcg tttttgccat
     4321 cggtggtcta ggagaaatcg ggaaaaacac ttacggaatt gaataccaag acgagattat
     4381 catcgtcgat gctgggatta aattcccaga agatgacttg cttggtatcg actatgtcat
     4441 tcctgactac tcttacatcg tggacaatat cgaccgcgtc aaggctgttt taatcacaca
     4501 cggacacgag gaccacattg gtgggattcc gttcctactc aagcaagcaa atgtccctat
     4561 ctacgctgga ccgcttgcct tggctttgat ccgtgggaaa ctcgaagaac acggcctctt
     4621 gcgcaacgcc aaactttacg aaatcaacca caacaccgag ttgaccttta aaaatctcaa
     4681 ggcaactttc tttagaacga ctcactctat tccagagcct ttggggattg tcattcatac
     4741 tcctcaaggg aaaatcgtct gtacgggtga ctttaagttc gactttactc cagttggaga
     4801 acctgcggac ttgcatcgta tggctgcgct tggtgaagaa ggcgtgctct gtctcctgtc
     4861 tgactcgaca aatgcggaag taccaacctt taccaactct gaaaaagtcg ttggtcagtc
     4921 cattatgaag attatccaag gtattgaagg acgtatcatc tttgcatcct ttgcctcaaa
     4981 tatcttccgt ctccagcagg caacagaagc tgctgttaag actggacgca agattgcggt
     5041 ctttggtcgt tctatggaaa aggccattgt caacggaatc gatcttggct acatcaaagc
     5101 tcctaaggga acctttatcg agccaaatga aatcaaagat tatcctgcag gagaagttct
     5161 tatcctctgt acaggtagtc agggtgagcc tatggcagcc ctctctcgta tcgccaacgg
     5221 aacccaccgt caagtacaat tacaaccagg tgataccgtt atcttctctt ctagtcccat
     5281 ccctggaaac actactagcg tcaacaagct gattaacatc atttctgaag ctggtgtcga
     5341 agttatccac ggtaaagtga acaatatcca tacatctgga cacggtggcc agcaagagca
     5401 aaaactcatg ctccgcttga ttaagccaaa atacttcatg cctgtccacg gtgaataccg
     5461 catgcaaaaa gtccacgctg gactagcagt ggatactggt gttgagaagg acaatatctt
     5521 tatcatgagc aatggcgatg tgcttgccct tactgctgac tcagctcgta tcgcaggtca
     5581 tttcaacgcc caagatatct atgtcgatgg aaatcgtatc ggtgaaattg gcgcagctgt
     5641 cctcaaagat cgtcgcgatc tatctgaaga cggtgtcgtt ctagcagtcg caactgttga
     5701 cttcaaatcg cagatgattc tgtctggccc agatatcctc agccgaggct ttgtctacat
     5761 gagagagtct ggagacttga ttcgccaaag ccagcgtatc ctcttcaatg ccattcgtat
     5821 cgcactgaaa aataaggatg ctagcgtgca atctgtcaat ggtgccattg tcaacgctat
     5881 tcgccccttc ctctatgaaa ataccgaacg tgaaccgatc atcatcccga tgatcctcac
     5941 accagatgaa gaataaagca agaaaacagc cccgtcctcg gagctgtttt tctctatgct
     6001 ttcttttgag attaaaactc atactcaatg a
//
LOCUS       DAVAAL010000109         5916 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVAAL010000109 DAVAAL010000000
VERSION     DAVAAL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.110"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(295..984)
                     /gene="rplA"
                     /locus_tag="VQM60_001667"
     CDS             complement(295..984)
                     /gene="rplA"
                     /locus_tag="VQM60_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET1081515.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1192..1617)
                     /gene="rplK"
                     /locus_tag="VQM60_001668"
     CDS             complement(1192..1617)
                     /gene="rplK"
                     /locus_tag="VQM60_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET1081516.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            complement(1740..2456)
                     /locus_tag="VQM60_001669"
     CDS             complement(1740..2456)
                     /locus_tag="VQM60_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET1081517.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(2474..2935)
                     /locus_tag="VQM60_001670"
     CDS             complement(2474..2935)
                     /locus_tag="VQM60_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET1081518.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            complement(2937..3974)
                     /locus_tag="VQM60_001671"
     CDS             complement(2937..3974)
                     /locus_tag="VQM60_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET1081519.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            complement(4072..5397)
                     /gene="brnQ"
                     /locus_tag="VQM60_001672"
     CDS             complement(4072..5397)
                     /gene="brnQ"
                     /locus_tag="VQM60_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET1081520.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5568..5660)
                     /locus_tag="VQM60_001673"
     CDS             complement(5568..5660)
                     /locus_tag="VQM60_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET1081521.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(5703..5855)
                     /locus_tag="VQM60_001674"
     CDS             complement(5703..5855)
                     /locus_tag="VQM60_001674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET1081522.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
BASE COUNT         1682 a         1335 c         1125 g         1774 t
ORIGIN      
        1 cgattcgtcc tattcttatt tcattttatt ataattgata gtggtcgccc cagccagata
       61 ccttatccgc tatataggcc ttattatcac gttcctgttc gtagagccac gatttcgcta
      121 tcccttcctc tgacgattct tatttccagc gtcagacttg ggaatcgcta ttgggttcac
      181 cggtagtagg tgcccaccga ggacttacac ctcagatata cgacatgccc gtcgtacatc
      241 aaaaaaagtt gagacatagt gtcttcaact tttttacatt actttaacta ctgattaaag
      301 tgagtttacg tcaactttga taccaacacc ttgagtagtt gtgatagtca agtttgttac
      361 gtaagttcct ttagctgtag ctggttttac tttttggatt gtttcgttga aagctttaaa
      421 gttttcaacc aatttttcag cttcaaatga tactttaccg atgattgctt gaacgttacc
      481 tgcacggtca gcacggtaag tgattttacc acctttagac tcttcaactg ctttagcaac
      541 atccattgtt acagtaccag ttttagggtt tggcatcaag ttacgtggtc caaggacacg
      601 tccaagacgt ccaacaagag ccatcatatc aggtgtagcg ataactacgt cgaagtccaa
      661 ccaaccgtcg ttgattttag caacaaggtc atcttcacca acaaagtctg caccagcagc
      721 ttttgcttct tcagcttttg caccacgtgc gaaaacaaga acacgtgaag ttttaccagt
      781 accgtttggc aataccattg ctccacggat ttgttggtca gcttttttaa cgtcgatgtt
      841 caagttgtaa gcaacttcta cagttgcatc aaattttgca aagttagttt cttttgcaag
      901 tgctacagct tcttctacac tgtatgcttt tgtgctgtcg attttctcaa gagcagcacg
      961 aagttgtttg ctttttttag ccattttcta ttctccttgt aagtggttca atcgattttc
     1021 atctcccacg tcacttctcg aaatgatgaa gtcttgcggg ttttcagtct actatttttc
     1081 tcttcctacg gccttaagct cccttgctgt atccaagtac aagcctcagt cacttgccta
     1141 gtatgaaaag caactaatag actgctgtga gattttctac tgtgttattg attagtcaac
     1201 aacagtgaat cccatagaac gagcagtacc ttcgatcata cgcattgcag actctacgtt
     1261 tgctgcgttc aaatctggca tcttagtttc tgcaatttct tgtacttgtg cacgagtaac
     1321 tgtagcaact ttagttttat taggtgtacc tgatcctttt tcaacacctg cagctttttt
     1381 caaaagaaca gcagctggtg gtgttttcgt aacgaaagta aatgatttat cttcgtaaac
     1441 tgagataaca actggaatga tcataccagc ttggtcagct gtacgagcgt tgaactcttt
     1501 tgtgaatccc atgatgttga taccagcttg accaagagca ggtccaaccg gtggagctgg
     1561 tgtagcttta ccagcaggga tttgcaattt tacaagtttt tcgacttttt tagccatttt
     1621 taaatcctcc tttgtggttt tggcggtaat tagagatttt tacctcccac aagtatgctt
     1681 tacacatacc tttccattat acactattta tctaaaaatg caagagaaaa ctttcatttt
     1741 taatcgacat aatcgccgcc ttcaaagcca tagtactctt ccaaactgag accacttgca
     1801 gcaatttctt tggcttcttt tccaacgtag cggagatgcc attcttctgc catgtagcca
     1861 gtctcctttt ccttgccttt aagatagcga acaacaaagc catagtcagc cgcatggtcc
     1921 aagagccact gggctgcctt ttcttcagta actaaatccc catctgtccc aatcaaatca
     1981 aaagcaagac ccgtctggtg ttcactgtaa cctgggcgtg ctgagtagcg atctgctgct
     2041 tccttaccat cttgattgac atagtcttgg tagagcttag cctgagtttc ataactccta
     2101 aacccactgt attggtcact gattggatag ccctcagcct gcatagctgc gattagtttg
     2161 agcaactctg ccttggctgt tggattttct cctggattgt aatcttttga caaaggataa
     2221 cgtttattag ctaggataat ctcatcgtat ttcccttgga tactataata gctcccttta
     2281 ttgctaactt ctgccttttt ctgactagaa gcttgagatt tgctacctac agttccttcc
     2341 ggttgactgc tttgctccgt tttttgagtg ttttcttcaa ctttggtttt ttcttgcgaa
     2401 caagctgcaa gggtcaatgc tagtaaacca gataaaatga catatctttt tttcatttct
     2461 ttcttcctct ctcttaggcc aaatacttct ccaattcatg agataagatt ttaatcatct
     2521 cttccagctc tggcgattgt acttggcaat cttctgccgc catttcttcc cagggcaccc
     2581 accagactgg gccacggcca ttcaaaccgt gactcttcat atcacctgct cttcggggaa
     2641 aaagatgcca atgagcgtgg gcatcgccat ttcctaggag ttcaatattc atcttttcag
     2701 cctcaaatgc tttggcgact gcttcttgga ctaaactcat ttcctctaaa aagcgaagtt
     2761 ttacagaagt ttccatatga tggagttctg tgacatgatc ttttgctaaa aataaggtat
     2821 agcccttaaa gtattggtag tcaccaataa caacatagcc tgtttctagt tcttttacaa
     2881 agtagggatt ttgcccagct ttaatgagtt cgattctctg acagattaag cacattctat
     2941 gataccgcct cgctcttaag ataagcatca accatacgct cagtcgctat tactgaatcg
     3001 atgtgggtgc gctcataaga gtgactggac tcaataccag ctccaagaag tgcatgcttg
     3061 acctctgcac ctgctgacat cgctgctgaa gcgtccgatc cgtaaaatgg atagatgtcg
     3121 agcttaaatg gaatatcttg ctctttagcc aaggcaacca agtgttgacg gaagtcatag
     3181 tgataaggac ctgaagcatc cttgacacag atagatactg tgtactcatc tgtctgctgg
     3241 tcatctccca tagctcccat atccacagcc agatattcta ctacctgagc aggaatgtta
     3301 gaattagcac catgtcctac ttcttcaaaa actgaaaaag caaaatgagt tgttactggc
     3361 aattcaatct tctctttctt ataaatacga agcaggttga gcaaaatcgc tgcgctgacc
     3421 ttatcatcca aatgacgaga cttgataaaa cctgtatcag tcacaacagt tcgtggatcg
     3481 aagctgataa aatcaccgac ctcaatccca agagcacgag tttctttttc attgcttact
     3541 tttacatcca aacgcacttc catattgtcc tgcgtgcgtt ctgcagttcc tgcatccttg
     3601 tagacatggc aagaagtttg gtggatgagg atggttcctg ataccttttg acctgtgcta
     3661 gccacatgaa cggtacagtt ttcaccttca atcatgttcc aaggaaaacc accgatacgg
     3721 tccaatttaa gacgaccatc tggtttgaca gcacgaacaa tagcacccag cgtatccaca
     3781 tgggcagtca catagcgatg ttgctcatca ttttgacctt tgatggtcac attgacacca
     3841 cccttagctg tgcgaactgg ctggtaacca aaaccttcta gagtcttgac taaataattc
     3901 gaaatttcac gagtgaagcc cgttggcgat gcgatggctg tcagttcttt gatatattct
     3961 actgtttgat tcatttcttc acctctttcg ctacctatta taacacattt atcatcggat
     4021 aaaaaaagaa aatcactcct gtagacttgg ctacaaaagt gatttaagtg attattccat
     4081 ttcaaaaaca tcgctttctt gcttgtttgg tagaaccaat gagagcaaga ttccaacaat
     4141 ggctggcacc aaccatggga gagatgcctt ggcaaaagga agaacgctaa caagatttgc
     4201 aagaaactca accttaaacg agcttcctag tacgcttgca atggcaataa ctgtaaccac
     4261 agcaattgtc aactgcatac ctggttttga aagggcaaca aatttgttga caatgacaat
     4321 cataacgata gcaatcgtga ttgggtacaa gataaccagt actggaattg agtacttgat
     4381 aatcgcatca agacccaaat tggcaatagc aaatccaatc aaggtaaagg ctgtcgcata
     4441 aaccttgtag ctgatttgtg ggaagcgctc attaaagaac tcagctgttg acacaatcaa
     4501 accaacagtc gttgtgaagc aggttacggt aaccatagct gcaaggaaga gttgagctgt
     4561 tgagccaaag atttcttgag tggcttgtga caagatgtaa acacctggtg ttccaccctt
     4621 catcgcttca gctggtactg ggaaatgatt tccaagaaaa cctaaaccga tgtaaagagc
     4681 gctgaaggca agggcaacaa cgataccaac aacccaaata gttgaaatgt attctttctt
     4741 acttgaaaat ccaagttgtt tcaaggtttg aactgcgatt acgctaaagg ccactgaagc
     4801 aagggcgtcc aaggtattgt aaccttctag gaaacctgta ccaaaggcag aagcttgata
     4861 agcagctgaa gcagcttgag gacttgttcc accatatttg atagctccca gaacgaccaa
     4921 gataacaatc aaaattgcaa agactggcgt taaaacacgt ccaatgcggt ctaagatttt
     4981 tgatggatta agcgaaatca aataggctgc cgcaaaatac agaaccgtaa atacaatcaa
     5041 gccaagtcct ttatttgcat ccgacaaaag ggggctaatc cctacttcgt aagctgttgt
     5101 agcagtacgt ggggtagcaa agaatggacc gattgacaag taaagaactg agaggtaaag
     5161 agtcgcaaac caaggcgcta tcttcgttga aatctcgtag atatatcctt taggatttag
     5221 cgttccaata ataagggtca agacggcgat accaacgcct gaaaagacaa aacctgcgat
     5281 ggcaggaaga aaatgttctc cagatagagc acctagagaa ggcggaaaaa tcaagttccc
     5341 cgcaccaaaa aatattccaa acaggagcaa acctgttagg gtaccttttt tagccataaa
     5401 aatctccttc atatttataa aatactgacc tagtatacca cgattaggag tctgaaaaaa
     5461 gcatcacgag gcaaatatta aatgttttca attttcttaa aaataagaat tatctaaaaa
     5521 taaaactctc ctacccagtc cacaccaagt aggagaaatg caaaatttta aagttcttca
     5581 aaggctaaca tgccaccctg gacattgata acattataac cttgttctaa caggaattgg
     5641 caagcacgcg ccgatctcat tccagatttg caaataatat aatgcaagcg atctttgtcc
     5701 aattaatcat aactatcagc caattgacta agcggtaggt tgtgggcaca ttctaaatga
     5761 agagctgcaa actcatccac ttctctcaca tccactagag aaagctggtc gttttggtaa
     5821 agctggtaaa atgcgtcaaa ggctatttct ttcattcttt ttcttctaat actcttcgaa
     5881 aatctcttca aaccacgtca gcgtcgcctt accgtg
//
LOCUS       DAVAAL010000110         5840 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVAAL010000110 DAVAAL010000000
VERSION     DAVAAL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.111"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..1304)
                     /gene="eno"
                     /locus_tag="VQM60_001675"
     CDS             complement(<1..1304)
                     /gene="eno"
                     /locus_tag="VQM60_001675"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET1081523.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            1468..1914
                     /locus_tag="VQM60_001676"
     CDS             1468..1914
                     /locus_tag="VQM60_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET1081524.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKIWEVKKEEPAKTSLWKKWFS"
     gene            complement(1911..3026)
                     /locus_tag="VQM60_001677"
     CDS             complement(1911..3026)
                     /locus_tag="VQM60_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET1081525.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3140..3364)
                     /locus_tag="VQM60_001678"
     CDS             complement(3140..3364)
                     /locus_tag="VQM60_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET1081526.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAE"
     gene            complement(3309..3680)
                     /locus_tag="VQM60_001679"
     CDS             complement(3309..3680)
                     /locus_tag="VQM60_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET1081527.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(3739..5172)
                     /gene="glgA"
                     /locus_tag="VQM60_001680"
     CDS             complement(3739..5172)
                     /gene="glgA"
                     /locus_tag="VQM60_001680"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET1081528.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGSQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5169..>5840)
                     /gene="glgD"
                     /locus_tag="VQM60_001681"
     CDS             complement(5169..>5840)
                     /gene="glgD"
                     /locus_tag="VQM60_001681"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET1081529.1"
                     /translation="KDISEVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDT
                     PWLIEHLEEEAKKEHPEKLRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDML
                     ESQKFYSLFSPNQKIYTKVKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIH
                     VHKDSLVKDSLLFPRVVIGEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVT
                     EDIHS"
BASE COUNT         1773 a         1283 c         1050 g         1734 t
ORIGIN      
        1 tattttttaa ggttgtagaa tgatttcaat ccacgatatt cagctacttc accaagttgg
       61 tcttcgatac gaagcaattg gttgtattta gcgatgcggt ctgtacgtga aagtgaacca
      121 gtcttgattt gtcctgcgtt agttgcaact gcaatatcag cgattgttga atcttcagtt
      181 tcacctgaac ggtgtgatac aacagcagtg taaccagctt ctttagccat ttcgatagct
      241 tcaaaagttt cagtaagagt accgatttgg ttaactttga taaggattga gttagcagca
      301 ccttcttgga taccacgtgc aaggtagtca gtgtttgtta cgaagaagtc gtcaccaaca
      361 agttgtactt tcttaccaag acgttcagta agagctttcc aaccatccca gtcattttca
      421 tccataccat cttcaatagt gatgattggg tatttgttaa ccaattcttc aaggtagtcg
      481 atttgttctg cagatgtacg aacagcagca ccttcacctt caaatttagt gtagtcgtaa
      541 actttacgtt ctttatcgta gaattctgat gaagcacagt caaatccgat aaatacgtct
      601 ttacctggta catatccagc agcttcaatc gcagcaagga tagtttcaac accatcttca
      661 gttccttcga aacgaggagc gaatccacct tcgtcaccta cggcagtttc caaaccacgt
      721 gatttaagga ttttcttaag agcgtggaag atttcagcac cgtaacgaag ggcttcttta
      781 aatgttggcg caccaactgg caagatcatg aactcttgga aagcgattgg agcgtcagag
      841 tgagaaccac cgttgatgat gttcatcatt ggagttggaa gaactttagt gttgaatcca
      901 ccaagatagc tgtaaagtgg gatttcaagg tagtcagcag cagcacgagc tacagcgata
      961 gacacaccga ggattgcatt cgcacccaat ttacctttgt taggagtacc gtcaagtgcg
     1021 atcatagcac ggtcaatagc ttgttgatca cgtacatcgt agccaatgat agcttcagca
     1081 atgatgttgt ttacgttgtc aacagctttt tgtgtaccaa gaccaccgta acgagatttg
     1141 tcaccgtcgc gaagttcaac tgcttcgtgt tcaccagtag aagctcctga tggaaccata
     1201 ccacgtccga aagcacctga ttcagtgtaa acttctactt caagtgttgg attaccgcgt
     1261 gagtctagga cttcgcgagc gtaaacatca gtaataattg acatttttta ctctccttat
     1321 gagttaaatt ttttacacct ctataatacc ttaaaacccc tcctttttca agaaaaaacg
     1381 ttatctttgt gcaacttttc cttaacttta taaagtaatc gctttctttt gtctgtttta
     1441 ttctaacttt tatgatatac tgttttcatg acagatttat caaaacaatt acttgaaaaa
     1501 gctcatggtg ggttaaaaat aaatccggat gagcaaagac gctatcttgg tacttttgag
     1561 gaaagagttc ttggatatgt agatattgac acagcaaata gccctcagtt agaaaaaggc
     1621 tttttattta ttttagaaaa ccttcaggaa aaagcagagc cactatttgt gaagatttca
     1681 ccaactatcg aatttgataa gcaagttttc tacttaaaag aagcaaaaga aactgatagt
     1741 caagccacca tagtatctga agagcatatt acttctcctt tcggcctggt tattcatagc
     1801 aatgcaccag ttcaagtaga agaaaaagac cttcgacttg cttttccaaa aatttgggaa
     1861 gttaaaaagg aagaaccagc caaaacatcc ttatggaaga aatggtttag ctaaatcttg
     1921 cacatattta ataagtgccc aatattggca gccgtgcgct ccagatagag actggcattt
     1981 ttcaaactat cttctaaagg ttcacttttc tccaaaatag aaaagacagc ttggatattt
     2041 tcaaatggta ggggaggtaa atcttcagca agactaccgc aaatagcaat aacaggaact
     2101 ccaacagggg ttctttttgc aacacctata ggcgctttcc cagcaaagct ttgactatca
     2161 agtcttcctt ctccaacaac aaccaagtca gcatctgaaa ctttcttatc aaagttgatt
     2221 aagtccaagc aggtatcaat tccagacacg atacttgcct gagcaaaggc acacaaacca
     2281 ccagcaaggc ctccacctgc tcctgctcct ttaatttcta atgttgcagg tgagaatttt
     2341 tcataaaaat cttggatcgc ctgatctacg actgcaaaca tagtcggatg tagacctttt
     2401 tgattgccaa aagtgtaagt cgcaccttga tgaccacata agggactcac gacatctgct
     2461 agaatatgaa tttgaacacc ttcaggaatt ttatagcaat tttctgttga aacagaagct
     2521 aagtttaata aggattgacc ggaagcaggc aagacatttc catccctatc ataaaattga
     2581 taacctaaac cagcagcaat ccccagtcct ccatcattac tggccgtgcc accaacaccg
     2641 atataaatat ctttaatccc tttagaaatg agatggagaa tcaactctcc aataccacaa
     2701 gtttggattt gaagtggatt tcgtttctct agcggaattt ttccaagacc aaccaagtca
     2761 gctacttcaa atagtgccag ttcccctttt tgaaaatagc gcatggcttc tttttgtcca
     2821 aaagggtctg tcacttggat ccatttttct tttaggtcaa gagaatgtcg gatagcatct
     2881 acagtacctt ctcccccatc accaacaggg cagaggagac attctacatc tgctatcgat
     2941 tgttggaagc ctctttttat tgcttcagct acctgttgag ctgtcaagct ttccttaaac
     3001 gaatccggtg caattacaat cttcatattt tccctcattc taaacagtca atcaaaggaa
     3061 gaacttctaa aaaatccctc ttgtcaacat gatgtggtat ttcttttttg agcatttctt
     3121 tggaacaaaa ggcgattcct cactccgccg acttcaacat taatagatta ttgaccccat
     3181 caccgattgc caccgttctt tctttagaaa gttttagttt ctttctccat ttttccagag
     3241 tttctttttt gacctgggga cttataattt gtccaactaa ttttcctgtt aaaagacctt
     3301 ctttgacttc aagctggttg gcagtgaaat aggcaatacc aagggatttt gctaatctct
     3361 ccaactattg gtgtaaatcc accagacacc agaccaacta ggatgccatt cttttggaga
     3421 atagagatga actctgggac atttagcgat agatgaattg agttgaagac gttatcaaag
     3481 accaaaatag gaagaccttc caacaaggac actctttttc ttaaactgct ttcaaagacc
     3541 aactctcctc gcattgctcg acttgtaatc tgcgaaattt ccgcctcatg acctgcctct
     3601 ctccctaaaa gatcaatcac ttcttctagg attaaggttc catctacatc caaaacacac
     3661 aagcctttta cttgagacat cagttctcct ctctaaacag cctaaaaatc gtatgaagtc
     3721 atcatacgat tttatctatt aattaactaa actatggtac aagtcaaggt atgacttgca
     3781 ggctgtatcc catgagaagt cactctccat agcttgtttt tgtaggtttc tccaaatgtc
     3841 tggatggttt ctatacaagt ccaatgctgt ttggaaagtc caatttaacc aataaggaga
     3901 tagattgtca aagctaaagc cagtaccgct tccttcgatt ggattgaaag cgcgaactgt
     3961 atctcgcaag cctccaactt catggaccaa tggcaaggtt ccataacgca tagccatcat
     4021 ttgagacaag ccacacggtt caaaacgact tggcatgagg aagaggtcac aagcagcgta
     4081 gatttcctga gcaagtttga catcaaaagt gatatttgtt gatagcttgt ctgggtaaat
     4141 ctgagcaaac catgagaaag ctccttcaaa ggctggatcg ccagttccca aaagaacaat
     4201 ctgaacatct tcttgcaaga tatggtgaag actttcgacc accacatcaa aacctttttg
     4261 acgtgtcaaa cgagaaacaa ttcccaccag tggaacgtct gctctaacag gcaagccaac
     4321 tctttcttgc aattttgcct tatttttggc tttcccagac aaatcttcct gattgaaatg
     4381 atagtctaaa agagcatccg tctgaggatt ataaagatca gcatcaatcc cattcacgat
     4441 accagatact ttaccagact ccattttaag aatctgatcc aaattacatc caaactgact
     4501 agtcataatt tcatgagcat agctaggtga aacagttgaa acacggttcg catagagaat
     4561 acctgccttc atccagttca gacagttgtt ccatcgaagg gtgccatcag cgtaacgttc
     4621 aaagccaact ccaaacaaat cacccaacat tccttctgaa aattgtcctt ggaattctaa
     4681 attatgaatg gttaaaactg tttcaatgtc ctcataggct tgaatccaac ggtatttttc
     4741 cttcaacaag aaaggaatca tagctgtatg gtagtcatga acatggagaa gatcaggaat
     4801 aaagtcaatc ctttccatag cctcaatggc agccagttgg aaaaaggcaa agcgttctcc
     4861 gtcatcaaaa tcaccgtaaa catgaccacg gaagaaataa tattgattgt caataaagta
     4921 gaaggttaca ccatttaata ctgttttctt aattccacaa tactgtctgc gccaaccaac
     4981 gctcacctca aaatgaagca catcttcaat ctgacttcca aatttagcct ctaccatatc
     5041 atagtagggt aaaatcactg caacttcgtg cccagctttt accagtgatt ttggaagagc
     5101 gccaatgacg tctcccaaac cacctgtttt tgaaaagggt gcaccctctg ctgctacaaa
     5161 taaaattttc atgaatgaat atcctctgtt actttagcac ctttcttaac cacaactgga
     5221 tgttctgcag ttcctcgaat cacaacacca ggctcaactt caaccccttt gtccaagata
     5281 gcatattcga cctgagcccc ttctccaata acaacacgag ggaagagcag gctatcttta
     5341 accaagctat ccttatggac atgaatatta cgtgatagaa cagaattagc tacttgacct
     5401 tcaataatac taccagaggc aaactgagaa gtgcttacct tagatgtatt agcatagtaa
     5461 gttggctctt cgtttttgac ctttgtataa atcttttggt ttggtgagaa aagagaatag
     5521 aatttttgtg attcaagcat atcgatattc gcttgataat aagatttaac agagtgaata
     5581 ttggctagat agcccgtgta ctcgtaggcg aaagctccct cttttacagc caaatcccgt
     5641 aaaacatagc gcaatttctc tggatgttct tttttagctt cttcttccaa gtgttcaatc
     5701 aaccaaggtg tatcaacgac aaagatatct gtagacatat tgaacgtttc agctgttgac
     5761 ttgctatcaa agagtttatg agaaagaaca tggtctgttt catctacatc caagattgca
     5821 tttacttctg aaatatcttt
//
LOCUS       DAVAAL010000111         5827 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVAAL010000111 DAVAAL010000000
VERSION     DAVAAL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.112"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            267..2591
                     /gene="pflB"
                     /locus_tag="VQM60_001682"
     CDS             267..2591
                     /gene="pflB"
                     /locus_tag="VQM60_001682"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET1081530.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIDPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDKYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(2649..3911)
                     /locus_tag="VQM60_001683"
     CDS             complement(2649..3911)
                     /locus_tag="VQM60_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HET1081531.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGGFPSLIHLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTVYRKLKQFHFQEDYTTLPEILSWDE
                     FSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSGSY
                     IPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFILK
                     DSRKLSLKPFYARTFRETLTPRECLKKIFTLVPELKDYYDLYQLLLFHLQEKNTDQFW
                     GLIQDTLPHLNRTFKTTLSTFIRYKNYITNAIELPYSNAKLEATNKLIKDIKRNAFGF
                     RNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(4176..5705)
                     /gene="rlrA"
                     /locus_tag="VQM60_001684"
     CDS             complement(4176..5705)
                     /gene="rlrA"
                     /locus_tag="VQM60_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HET1081532.1"
                     /translation="MLNKYIEKRITDKITILNILLDIRSIELDELSTLTSLQSKSLLS
                     ILQELQETFEEELTFNLDTQQVQLIEHHSHQTNYYFHQLYNQSTILKILRFFLLQGNQ
                     SFNEFTQKEYISIATGYRVRQKCGLLLRSVGLDLVKNQVVGPEYRIRFLIALLQFHFG
                     IEIYDLNDGSMDWVTHMIVQSNSQLSHELLEITPDEYVHFSILVALTWKRREFPLEFP
                     ESKEFEKLKNLFMYPILMEHCQTYLEPHANMTFTQEELDYIFLVYCSANSSFSKDKWN
                     QEKKTHTIQLILQHTRGKHLLSKFKNILGNDISNSLSFLTALTFLTRTFLFGLQNLVP
                     YYNYYEHYGIESDKPLYHISKAIVQEWMTEQKIEGVIDQHRLYLFSLYLTETIFSSLP
                     AIPIFIILNNQADVNLIKSIILRNFTDKVASVTGYNILISPPPSEEHLTEPLIIITTK
                     EYLPYVKKQYPKGKHHFLTIALDLHVSQQRLIYQTIVDIRKEAFDKRVAMIAKKAHYL
                     L"
BASE COUNT         1817 a         1035 c         1170 g         1805 t
ORIGIN      
        1 aatctgcggc tagcttccta gtttgctttt cgatttccat tgagtgttac tgcttatttt
       61 cttttattat accctttttt ctgaaaaaaa gaaaaaagga ctttattttt tcaaaaatat
      121 aatacagttt gaaataaaat atagactgtt ttagaaaaga aagtgtaaaa atagggattt
      181 ttcacttgtt gaaatcggtt actatatggt atacttgtct catgaatgta acagatgact
      241 gttactagaa aaaagaggac attaatatgg ttgttaagac agttgttgaa gcacaagata
      301 tttttgacaa agcttgggaa ggcttcaaag gcgtagattg gaaagaaaaa gcaagtgtat
      361 cacgctttgt acaagctaac tacacacctt atgatggaga cgaaagcttc cttgcaggac
      421 caacagagcg ttcacttcac atcaagaaaa ttgtagaaga aactaaagca cactacgaag
      481 aaactcgttt cccaatggac actcgtccaa catctatcgc tgatatccct gctggattta
      541 tcgacaaaga aaatgaagtt atctttggta tccaaaatga tgaactcttc aaattgaact
      601 tcatgccaaa aggtggtatc cgtatggctg aaactacttt aaaagaaaat ggatacgaac
      661 cagacccagc tgttcacgaa atcttcacta aatatgtaac aacagttaac gacggtattt
      721 tccgtgccta cacttcaaat attcgtcgcg ctcgtcatgc acacactgta actggtcttc
      781 cagatgcata ctcacgcgga cgtatcatcg gtgtttacgc acgtcttgct ctttacggtg
      841 cagactactt gatgcaagaa aaagtaaacg actggaatgc aatcaaagaa atcgatgaag
      901 aaacaatccg tcttcgtgaa gaagtaaacc ttcaatacca agcattgcaa caagttgttc
      961 gcctgggtga cctttacggg gttgatgttc gcaaaccagc gatgaacgtg aaagaagcaa
     1021 tccaatgggt taacattgct ttcatggctg tctgccgtgt gattaacggt gctgctacat
     1081 ctctaggtcg tgtaccaatc gtattggaca tctttgcaga acgtgacctt gctcgtggta
     1141 catttactga atcagaaatc caagaattcg ttgatgattt cgttatgaaa cttcgtacag
     1201 ttaaatttgc tcgtaccaaa gcttatgacc aattgtactc aggtgaccca acctttatca
     1261 caacttctat ggctggtatg ggtaacgacg gtcgtcaccg tgttactaag atggactacc
     1321 gtttcttgaa cactcttgac aacatcggta actcaccaga accaaacttg acagttcttt
     1381 ggactgacaa attgccatac aacttccgtc gctactgtat gcacatgagc cacaaacact
     1441 cttctatcca atacgaaggt gtaacaacaa tggctaaaga cggatatggt gaaatgagct
     1501 gtatctcatg ctgtgtgtct ccacttgatc cagaaaatga agaacaacgc cacaacatcc
     1561 agtacttcgg tgctcgtgta aacgttctta aagcccttct tactggtttg aatggtggtt
     1621 acgacgatgt tcacaaagac tacaaagtat ttgacatcga tcctattcgt gacgaagttc
     1681 ttgaatttga atcagttaaa gcgaactttg aaaaatctct tgactggttg actgacactt
     1741 acgtagatgc cttgaacatc atccactaca tgactgataa gtacaactac gaagctgttc
     1801 aaatggcctt cttgccaact aaacaacgtg ccaacatggg attcggtatt tgtggatttg
     1861 ctaacactgt tgatacattg tcagcgatta aatacgctac agttaaacca atccgtgatg
     1921 aagatggcta catctacgat tacgaaacaa tcggtgacta cccacgctgg ggtgaagatg
     1981 acccacgttc aaacgaattg gcagaatggt tgatcgaagc ttacacaact cgtctacgta
     2041 gccacaaact atacaaagac gcagaagcta cagtatcact tttgacaatc acatctaacg
     2101 ttgcttactc taaacaaact ggtaactcac cagttcacaa aggtgtatac ctcaacgaag
     2161 atggttctgt gaacttgtct aaacttgaat tcttctcacc aggtgctaac ccatctaaca
     2221 aagctaaagg tggttggttg caaaacttga actcactttc tagccttgac tttagttatg
     2281 cagctgacgg tatctcattg actacacaag tatcacctcg cgctcttggt aagactcgtg
     2341 atgaacaagt tgataacttg gtaacaatcc ttgatggtta cttcgaaaac ggtggacaac
     2401 acgttaactt gaacgttatg gacttgaacg atgtttacga aaaaatcatg tcaggcgaag
     2461 acgttatcgt acgtatctct ggatactgtg taaacactaa atacctcact ccagaacaaa
     2521 aaactgaatt gacacaacgt gtcttccacg aagttctttc aatggatgac gccttggatg
     2581 cattgagcta atcaagttct tgaataataa aaagggctct ttgtcaactg tagtgggttg
     2641 aagaaaagct aagctcgaga aaggacaaat tttgtccttt cttttttgat gttcagagcg
     2701 atgaaaatcc gttttttgaa gttttcaaag ttccgaaaac caaaggcatt gcgcttgatg
     2761 tctttgatga gtttgttagt ggcctcaagt ttagcgttag aataaggcaa ttcaatggcg
     2821 ttagtgatgt agtttttata gcgaataaat gtgctcaaag tggttttaaa ggtgcggttg
     2881 agatgaggta acgtgtcttg aattaagccc caaaactggt cagtattctt ctcttgtaga
     2941 tgaaatagga gtagttgata caggtcatag taatctttaa gttcaggtac tagagtaaag
     3001 attttcttca gacactccct aggagttaag gtctctctga aagttctagc atagaaaggc
     3061 ttaagagaga gtttccgact atcttttagg ataaatttcc agtaatattt aagagctctg
     3121 tattccagag atttatcatc aaattgcttc atgatgttga ttctagtctg attaagagcc
     3181 ctgctcatgt gttggacaat gtggaaacga tcgagaacaa ttttagcatt gggaaataat
     3241 ttcttaatga gagggatata acttccagac atatcaacag tgacgacttt aacttttttt
     3301 ctagcttctt tcgagtactt gaagaaatga tttcggatgg ttgtttgacg tctgttatca
     3361 agaatggtca tgattttctt agtgttgaaa tcctgagcaa tgaaagccaa tttccccttc
     3421 tggtaggaga attcatccca ggagaggatt tcaggcaaag tggtgtaatc ctcttggaaa
     3481 tgaaattgct tgagcttacg atagacggta gaggtagagg tagagatggc taatttagaa
     3541 gcgatatgtg taagagcctc tctgttgagt aggagttggg caattttctg tctcaccatt
     3601 tccgagattt ggcaattttt ctgaacgaga gttgtttcag ctacagtgac tttccgacag
     3661 gacttgcatt gaaatcgtct ctttttcaaa tgaatgaggc tagggaaacc accaatctcg
     3721 ataaaaggga ttttagaagg cttttggaag tcgtatttga tttgttttcc tttacagtgt
     3781 ttacatttag gtgggtgata atcaagtgta gcgaagactt cgatatgggt atcgtgctga
     3841 atggctttat ttaaggtgat gtttttgtct tttattccga tgagtaatgt ggtatgattg
     3901 atgtgttcca taagatactt tctaatgagt tgtttaggcg cttttcatta taaatcttat
     3961 gggacttttt tgatactcaa aaagccctat aatctccaca gtgggattta cccactacag
     4021 aaattataga gccagaaaaa acacttttgt tcactagcag aaactagaga gcagaagtgt
     4081 ttttctgttc agatttaccc aaaactggga aatatgggga taagaataga gatggcttag
     4141 gaagcccctt tttgtgtgta gacagtacga tgaacttata acaaatagtg agccttttta
     4201 gcaatcattg cgacccgttt gtcaaaagcc tcttttcgga tatctacaat tgtctgatag
     4261 atgagacgct gttggctaac atgcaaatct aaggcaatcg tcaaaaagtg atgtttccct
     4321 ttgggatact gctttttaac gtaaggcagg tattctttcg ttgtaataat aatcaatggc
     4381 tctgtcaaat gctcctctga aggaggagga ctaattagaa tattgtatcc tgtaacagag
     4441 gcaactttgt cagtaaaatt ccgtaaaata atggacttta ttaagtttac atctgcttga
     4501 ttatttaaaa tgataaaaat cgggatagca ggtagtgagg aaaagatggt ttctgtcaag
     4561 tagagtgaga aaaggtacag ccgatgctgg tcgataactc cttcaatctt ctgctcagtc
     4621 atccactctt gaacaattgc tttcgaaata tgatacagtg gcttgtcgct ttcaatccca
     4681 taatgttcgt aataattata atagggaact agattttgta aaccaaacaa aaacgttctt
     4741 gttaagaaag tcagtgctgt taaaaaagaa agagaattcg aaatgtcatt tcctaagata
     4801 ttcttgaact tggatagtag atgctttcct cttgtatgct gaagaatcag ttgaatagta
     4861 tgagtctttt tttcttgatt ccatttgtcc ttggaaaacg aagaattagc agaacaataa
     4921 accaaaaaga tataatccag ttcttcctga gtaaaagtca tgttggcatg tggctctaag
     4981 taagtttggc aatgttccat caaaatcgga tacataaaga ggttttttaa tttttcaaac
     5041 tctttggact cagggaactc aagtggaaat tcccgacgtt tccaagtgag tgccactagt
     5101 atgctaaaat gaacatactc gtcaggtgtg atttctaaca gttcatgact gagttgagaa
     5161 ttagactgca caatcatatg tgtgacccaa tccatacttc catcattcaa atcataaatc
     5221 tcaataccaa aatgaaactg gaggagtgca attaaaaaac gaatgcgata ttcaggacca
     5281 actacttgat ttttcacaag gtccaaacct actgaacgta gtaacaagcc acacttttgt
     5341 cgtacgcggt agcctgttgc gatggaaata tactcttttt gtgtaaattc gttaaagctt
     5401 tgattacctt gtagtagaaa gaagcggagt atttttaaaa tagttgattg gttataaagc
     5461 tgatggaagt aataattcgt ttgatgagaa tggtgttcga ttaattgaac ttgttgcgta
     5521 tctaaattaa atgtcaactc ttcctcgaat gtttcttgta attcctgcaa aatgcttagg
     5581 agacttttag attgtaatga agttaaagta gacagttcat ctagttcaat agaccgaata
     5641 tccaataata tatttaaaat ggtaatttta tctgtaattc ttttttcaat gtatttgttt
     5701 agcatagtta ccgaatctta gttgcatata gataatttta attattataa tacaaaagaa
     5761 actaattgtc ttgtcaaaaa ggttgtggaa tttccgactt tattgataaa acagcatgta
     5821 ataaaag
//
LOCUS       DAVAAL010000112         5800 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVAAL010000112 DAVAAL010000000
VERSION     DAVAAL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.113"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            121..1666
                     /locus_tag="VQM60_001685"
     rRNA            121..1666
                     /locus_tag="VQM60_001685"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1718..1790
                     /locus_tag="VQM60_001686"
     tRNA            1718..1790
                     /locus_tag="VQM60_001686"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1751..1753,aa:Ala,seq:tgc)
     gene            1915..4816
                     /locus_tag="VQM60_001687"
     rRNA            1915..4816
                     /locus_tag="VQM60_001687"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4893..5008
                     /gene="rrf"
                     /locus_tag="VQM60_001688"
     rRNA            4893..5008
                     /gene="rrf"
                     /locus_tag="VQM60_001688"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            5013..5085
                     /locus_tag="VQM60_001689"
     tRNA            5013..5085
                     /locus_tag="VQM60_001689"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5046..5048,aa:Val,seq:tac)
     gene            5088..5160
                     /locus_tag="VQM60_001690"
     tRNA            5088..5160
                     /locus_tag="VQM60_001690"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5121..5123,aa:Asp,seq:gtc)
     gene            5185..5257
                     /locus_tag="VQM60_001691"
     tRNA            5185..5257
                     /locus_tag="VQM60_001691"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5218..5220,aa:Lys,seq:ttt)
     gene            5263..5344
                     /locus_tag="VQM60_001692"
     tRNA            5263..5344
                     /locus_tag="VQM60_001692"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5297..5299,aa:Leu,seq:tag)
     gene            5363..5435
                     /locus_tag="VQM60_001693"
     tRNA            5363..5435
                     /locus_tag="VQM60_001693"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5396..5398,aa:Thr,seq:tgt)
     gene            5458..5529
                     /locus_tag="VQM60_001694"
     tRNA            5458..5529
                     /locus_tag="VQM60_001694"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5490..5492,aa:Gly,seq:gcc)
     gene            5537..5622
                     /locus_tag="VQM60_001695"
     tRNA            5537..5622
                     /locus_tag="VQM60_001695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5571..5573,aa:Leu,seq:taa)
     gene            5632..5705
                     /locus_tag="VQM60_001696"
     tRNA            5632..5705
                     /locus_tag="VQM60_001696"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5666..5668,aa:Arg,seq:acg)
     gene            5721..5794
                     /locus_tag="VQM60_001697"
     tRNA            5721..5794
                     /locus_tag="VQM60_001697"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5755..5757,aa:Pro,seq:tgg)
BASE COUNT         1587 a         1197 c         1706 g         1310 t
ORIGIN      
        1 agacctttga aaactgaaca agacgaacca atgtgcaggg cgctacaaca taagttgtag
       61 tactgaacaa tgaaaaaaca ataaatctgt cagtgacaga aatgagtgag aactcaaact
      121 tttaatgaga gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa
      181 gtagaacgct gaaggaggag cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg
      241 taggtaacct gcctggtagc gggggataac tattggaaac gatagctaat accgcataag
      301 agtggatgtt gcatgacatt tgcttaaaag gtgcacttgc atcactacca gatggacctg
      361 cgttgtatta gctagttggt ggggtaaggg ctcaccaagg cgacgataca tagccgacct
      421 gagagggtga tcggccacac tgggactgag acacggccca gactcctacg ggaggcagca
      481 gtagggaatc ttcggcaatg gacggaagtc tgaccgagca acgccgcgtg agtgaagaag
      541 gttttcggat cgtaaagctc tgttgtaaga gaagaacgag tgtgagagtg gaaagttcac
      601 actgtgacgg tatcttacca gaaagggacg gctaactacg tgccagcagc cgcggtaata
      661 cgtaggtccc gagcgttgtc cggatttatt gggcgtaaag cgagcgcagg cggttagata
      721 agtctgaagt taaaggctgt ggcttaacca tagtaggctt tggaaactgt ttaacttgag
      781 tgcaagaggg gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa
      841 caccggtggc gaaagcggct ctctggcttg taactgacgc tgaggctcga aagcgtgggg
      901 agcaaacagg attagatacc ctggtagtcc acgctgtaaa cgatgagtgc taggtgttag
      961 accctttccg gggtttagtg ccgtagctaa cgcattaagc actccgcctg gggagtacga
     1021 ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt
     1081 ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac atccctctga ccgctctaga
     1141 gatagagttt tccttcggga cagaggtgac aggtggtgca tggttgtcgt cagctcgtgt
     1201 cgtgagatgt tgggttaagt cccgcaacga gcgcaacccc tattgttagt tgccatcatt
     1261 tagttgggca ctctagcgag actgccggta ataaaccgga ggaaggtggg gatgacgtca
     1321 aatcatcatg ccccttatga cctgggctac acacgtgcta caatggctgg tacaacgagt
     1381 cgcaagccgg tgacggcaag ctaatctctt aaagccagtc tcagttcgga ttgtaggctg
     1441 caactcgcct acatgaagtc ggaatcgcta gtaatcgcgg atcagcacgc cgcggtgaat
     1501 acgttcccgg gccttgtaca caccgcccgt cacaccacga gagtttgtaa cacccgaagt
     1561 cggtgaggta accgtaagga gccagccgcc taaggtggga tagatgattg gggtgaagtc
     1621 gtaacaaggt agccgtatcg gaaggtgcgg ctggatcacc tcctttctaa ggataaggaa
     1681 ctgcgcattg gtcttgttta gtcttgagag gtcttgtggg gccttagctc agctgggaga
     1741 gcgcctgctt tgcacgcagg aggtcagcgg ttcgatcccg ctaggctcca ttggtgagag
     1801 atcaccaagt aatgcacatt gaaaattgaa tatctatatc aaatagtaac aagaaaataa
     1861 accgaaaacg ctgtagtatt aataagagtt tatgactgaa aggtcagaaa ataaggttaa
     1921 gttaataagg gcgcacggtg gatgccttgg cactaggagc cgacgaagga cgtgacaaac
     1981 gacgatatac cttgggtagc tgtaagtaag cgatgatcca gggatttccg aatgggggaa
     2041 cccaacaggt aatacctgtt acccacatct gttaaggatg tgaggaggaa gacgcagtga
     2101 actgaaacat ctaagtagct gcaggaagag aaagcaaaag cgattgcctt agtagcggcg
     2161 agcgaaacgg cagaagggca aaccgaagag tttactcttc ggggttgtag gactgcaatg
     2221 tggactcaaa gattatagaa gaatgatttg ggaagatcag ccaaagagag taatagcctc
     2281 gtatttaaaa tagtctttgt acctagcagt atcctgagta cggcgggaca cgtgaaatcc
     2341 cgtcggaatc tgggaggacc atctcccaac cctaaatact ccctagtgac cgatagtgaa
     2401 ccagtaccgt gagggaaagg tgaaaagcac cccgggaggg gagtgaaata gaacctgaaa
     2461 ccgtgtgcct acaacaagtt cgagcccgtt aatgggtgag agcgtgcctt ttgtagaatg
     2521 aaccggcgag ttacgttatg atgcgaggtt aagttgaaga gacggagccg tagggaaacc
     2581 gagtctgaac agggcgaatt agtatcatga cgtagacccg aaaccatgtg acctacccat
     2641 gagcaggttg aaggtgcggt aagacgcact ggaggaccga accagggcac gttgaaaagt
     2701 gcttggatga cttgtgggta gcggagaaat tccaaacgaa cttggagata gctggttctc
     2761 tccgaaatag ctttagggct agcgtcgaca ttagagattc ttggaggtag agcactgttt
     2821 gggtgagggg tccatcccgg attaccaatc tcagataaac tccgaatgcc aatgaattat
     2881 ggtcggcagt cagactgcga gtgctaagat ccgtagtcga aagggaaaca gcccagacca
     2941 ccagctaagg tcccaaaata attgttaagt ggaaaaggat gtggggttgc acagacaact
     3001 aggatgttag cttagaagca gctattcatt caaagagtgc gtaatagctc actagtcgag
     3061 tgaccctgcg ccgaaaatgt accggggcta aaacaattta ccgaagctgt ggataccttt
     3121 ataggtatgg taggagagcg ttctatgtgt gatgaaggta taccgtgagg agtgctggaa
     3181 cgcatagaag tgagaatgcc ggtatgagta gcgaaagaca ggtgagaatc ctgtccaccg
     3241 taagactaag gtttccaggg gaaggctcgt ccgccctggg ttagtcggga cctaaggaga
     3301 gaccgaaagg tgtatccgat ggacaacagg ttgatattcc tgtactagag tatgtagtga
     3361 tggagggacg cagtaggcta actaaagcag acgattggaa gagtctgtct aagcagtgag
     3421 gtgtgaattg agtcaaatgc ttaattctat aacattgagc tgtgatgggg agcgaagttt
     3481 agtagcgaag ttagtgacgt cacactgcca agaaaagctt ctagcgttta aacatactct
     3541 acccgtaccg caaaccgaca caggtagtcg aggcgagtag cctcaggtga gcgagagaac
     3601 tctcgttaag gaactcggca aaatgacccc gtaacttcgg gagaaggggt gctgacttta
     3661 ggtcagccgc agtgaatagg cccaagcaac tgtttatcaa aaacacagct ctctgctaaa
     3721 tcgtaagatg atgtataggg ggtgacgcct gcccggtgct ggaaggttaa gaggagtgct
     3781 tagcgtaagc gaaggtatga attgaagccc cagtaaacgg cggccgtaac tataacggtc
     3841 ctaaggtagc gaaattcctt gtcgggtaag ttccgacccg cacgaaaggc gtaatgattt
     3901 gggcactgtc tcaacgagag actcggtgaa attttagtac ctgtgaagat gcaggttacc
     3961 cgcgacagga cggaaagacc ccatggagct ttactgcagt ttgatattga gtgtctgtac
     4021 cacatgtaca ggataggtag gagtctaaga gatcgggacg ccagtttcga aggagacgct
     4081 gttgggatac tacccttgtg ttatggccac tctaacccag ataggtgatc cctatcggag
     4141 acagtgtctg acgggcagtt tgactggggc ggtcgcctcc taaaaggtaa cggaggcgcc
     4201 caaaggttcc ctcagaatgg ttggaaatca ttcgcagagt gtaaaggtat aagggagctt
     4261 gactgcgaga gctacaactc gagcagggac gaaagtcggg cttagtgatc cggtggttcc
     4321 gtatggaagg gccatcgctc aacggataaa agctaccctg gggataacag gcttatctcc
     4381 cccaagagtt cacatcgacg gggaggtttg gcacctcgat gtcggctcgt cgcatcctgg
     4441 ggctgtagtc ggtcccaagg gttgggctgt tcgcccatta aagcggcacg cgagctgggt
     4501 tcagaacgtc gtgagacagt tcggtcccta tccgtcgcgg gcgtaggaaa tttgagagga
     4561 tctgctccta gtacgagagg accagagtgg acttaccgct ggtgtaccag ttgtcttgcc
     4621 aaaggcatcg ctgggtagct atgtagggaa gggataaacg ctgaaagcat ctaagtgtga
     4681 aacccacctc aagatgagat ttcccatgat tatatatcag taagagccct gagagatgat
     4741 caggtagata ggttagaagt ggaagtgtgg cgacacatgt agcggactaa tactaatagc
     4801 tcgaggactt atccaaagta actgagaata tgaaagcgaa cggttttctt gaattgaata
     4861 gatattcaat tttgagtagg tattactcag agttaagtga cgatagccta ggagatacac
     4921 ctgtacccat gccgaacaca gaagttaagc cctagaacgc cggaagtagt tgggggttgc
     4981 cccctgtgag atagggaagt cgcttagctc tagggagttt agctcagctg ggagagcatc
     5041 tgccttacaa gcagagggtc agcggttcga tcccgttaac tcccaaaggt cccgtagtgt
     5101 agcggttatc acgtcgccct gtcacggcga agatcgcggg ttcgattccc gtcgggaccg
     5161 tttaaggtaa cgcaagttat tttagactcg ttagctcagt tggtagagca attgactttt
     5221 aatcaatggg tcactggttc gagcccagta cgggtcatat atgcgggttt ggcggaattg
     5281 gcagacgcac cagatttagg atctggcgct taacggcgtg ggggttcaag tcccttaacc
     5341 cgcattaaga tataacaaat gagccggctt agctcagttg gtagagcatc tgatttgtaa
     5401 tcagagggtc gcgtgttcaa gtcatgtagc cggcattttt agatagaaga aaacagtgcg
     5461 aacgtagttc agtggtagaa caccaccttg ccaaggtggg ggtcgcgggt tcgaatcccg
     5521 tcgttcgctt agagaggccg gggtggcgga actggcagac gcacaggact taaaatcctg
     5581 cgattggtaa cgatcgtacc ggttcgattc cggtcctcgg cataatatgg agcaccctta
     5641 gctcaactgg atagagtacc tgactacgaa tcaggcggtt agaggttcga ctcctctagg
     5701 gtgcattttt ctatttaact cgggaagtag ctcagcttgg tagagtactt ggtttgggac
     5761 caaggtgtcg caggttcgaa tcctgtcttc ccgattcatt
//
LOCUS       DAVAAL010000113         5760 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVAAL010000113 DAVAAL010000000
VERSION     DAVAAL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.114"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..984)
                     /locus_tag="VQM60_001698"
     CDS             complement(<1..984)
                     /locus_tag="VQM60_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET1081533.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELTQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAAL"
     gene            complement(975..1673)
                     /locus_tag="VQM60_001699"
     CDS             complement(975..1673)
                     /locus_tag="VQM60_001699"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET1081534.1"
                     /translation="MKELQTVLKNHFAIEFTDKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(1856..1927)
                     /locus_tag="VQM60_001700"
     tRNA            complement(1856..1927)
                     /locus_tag="VQM60_001700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1892..1894),aa:Arg,seq:ccg)
     gene            complement(1991..2977)
                     /locus_tag="VQM60_001701"
     CDS             complement(1991..2977)
                     /locus_tag="VQM60_001701"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET1081535.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(3152..4468)
                     /locus_tag="VQM60_001702"
     CDS             complement(3152..4468)
                     /locus_tag="VQM60_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET1081536.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLYINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFFVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKTEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(4455..5672)
                     /locus_tag="VQM60_001703"
     CDS             complement(4455..5672)
                     /locus_tag="VQM60_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET1081537.1"
                     /translation="MDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTD
                     GNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQLIGG
                     QDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYE
                     LYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEIS
                     KIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDRSVDTFD
                     FAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSHYHIGPS
                     YFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQKDQAVA
                     DDNEGDENDDADY"
BASE COUNT         1689 a         1256 c          965 g         1850 t
ORIGIN      
        1 caaagcagcc aaacgggctt gtgcctcctg ttgattcaag gccacttgct cggactccag
       61 tttcgatagg gctaattttc tttctaaatc actaatcaga ctagtcaagt ccatcaaact
      121 gccttggtct ttggccattt cagcctgtaa atcttggcgt tgctttttaa gagtttgatt
      181 ttcttcttct aatttttcac gcttttggta ataactcatc aagagttctt gaacctgagt
      241 caactcttct tctgtcgact ctagttcagc cttattttcc ttgatttgag caaccagaac
      301 atctaaataa atagccttac gttgtccttc caagtctaaa aacttacggg cattctcagc
      361 ttgcttctca agaggcttga tttgattatc caactcgtag ataatgtcct ctaagcggtc
      421 cagattatcc tgagtttgtt gcagtttact ctcagtttct tttctacgag tcttgtattt
      481 taaaactcct gcagcttctt caaaaatagc tcggcgttcc tcaggcttag aattaaaaat
      541 ctcctcaacc ttgccttggg aaatgataga gaaggaatct cgtcccaatc cagtatccaa
      601 gaagaggtca tgaatatcac gcagacggac tttcttgccg tcaatcttgt attcgctatc
      661 tccactacga tagatatggc gttccaccct gatttcttga cctgcatcct tgataaatcc
      721 gtcatgatta tccagagtca caactacaga agcataattg agcggtttgc gactttcggt
      781 tccagcaaag atgacatccg gcatcttgcc cccacggaga ctcttgacac tagactcccc
      841 caaagcccaa cgcagacttt ctgtaatatt ggactttcca gatccattgg gtccaacaac
      901 tgccgtcaca ccttggtcaa aaacgacctt ggtcttatca gcaaaagact tgaacccctg
      961 aatttcgatt tcctttaaat acatgaatcc agccccttct caacggcatt tttggcagct
     1021 tcctgctctg ctaatttctt agaacgacct tggccttgac cgatgctctt accttcaaca
     1081 agaacttcta catcaaagac cttatcgtga gcaggccctg tttcagaaat cacctgataa
     1141 cgaatagcca catcaccatt gacctgaagc aactcttgga gatgggtttt atagtctgta
     1201 atcatctcaa actcgcctgc ttcaacctta ggaatcatga cttgatagat aaattccttg
     1261 accttggcca catccttatc caaaagaagg gcaccaagaa aggcttcaaa ggcatcacca
     1321 agaatggtgt cacgattgcg accaccagat ttttcttccc ctttacccaa cttgataaac
     1381 tggtcaaact ggcaatcacg cgcaaaacca gctaaactct cctcacggac aatcatagca
     1441 cggagttttg ataggtcacc ttcaggcttt ttaggatatt ttttatacag atattctgaa
     1501 atcaataact gtagaacagc gtctcctaaa aattccaagc gttcattgtg tgaaattttt
     1561 aagaggcggt gctcattggc ataactcgta tgagtaaagg cagtttccag taaatttttg
     1621 tctgtaaatt cgattgcaaa atggttcttt agtacagttt gtaattcttt cataccaacc
     1681 tctttctaac tgataacagt cctttttatt atatcaaaaa aagccccctg agtcactcta
     1741 aaacgggact ggaaagcatt tgggaattct ttagacagag attctcagtt ttagcggcaa
     1801 atttagatca gtgtaaagaa aaaagcccta ttaaaggctt tttaggatgt ttacatccac
     1861 cctgagggaa tcgaaccccc atctcaagaa ccggaatctt acgtgatatc cattacacta
     1921 agggtggaaa cttgttttat tataacagaa atttgctcta ataacaagtt ttttagtcaa
     1981 agcccccgtc ttagtgggaa gcatccccat tccagatgga gtttttcacg atcacataat
     2041 caacgtgttt aaggtcagca acctgacgtc cacctgcata agaaatagca ctttgaaggt
     2101 cttgttccat ctcagttaaa gtgtcttgca gatgaccttt agcaggaagc aagatacgtt
     2161 tgccttccac atttttgtaa gcaccttttt gatattgtga ggctgaacca taatattctt
     2221 tgaactgttc accatcgact tcaatcgttt tccctggact ttcaatgtgt cctgcaaaga
     2281 gggaaccaat catgatcatg ctagcaccga agcggataga cttggcaata tcaccgtgag
     2341 tacgaattcc tccatcagcg ataatcggtt tacgcgcagc cttggcacac cagcgtagag
     2401 cagccaactg ccaaccacct gtaccaaaac cagtcttaac cttggtgata caaaccttac
     2461 caggaccgat tccgacctta gtagcatccg caccagcatt ttccaattca cgcacagctt
     2521 ctggtgttcc cacatttcca gcaatgacaa aggtatctgg caattctttc ttgatgtgtt
     2581 gaatcataga aatcacgcta tccgcatgac catgagcaat atcaatagtg atatactcag
     2641 gagcatcagt cttgagctgt ctaacgaaat catactcata gtccttaaca ccgacagaga
     2701 tagaagcaat gagcccttga tcgtgcattc gtttaataaa aggaatgcgt cctgcctcat
     2761 caaaacggtg cataatgtag aagtaaccac ctttagccag ttgctctgct acattttcat
     2821 ccaaaatcgt ctgcatattc gctggcacaa caggtagttt aaaggtgtga tttcctaaag
     2881 tgacacttgt atccgcttct gcacggcttt taatgacaca tttatttgga atcaattgaa
     2941 tatcttcgta atcaaaaatt ggaaattcat ttaacatatc gatgtctcgt ttcttttgta
     3001 atgacctacc tatgctctcg catcactacg tcttttccga cgtttcctta atttattata
     3061 aaccaaagta cagtttttgt caaattattt tatgaattaa aatatatcgt tcgggtttta
     3121 cttgtgttaa aaataaaaga aagaggggaa attattttct ccccacttct tcatcaataa
     3181 tcgctttaaa attttcttct gaattttcca tcattttaca aaatgcacta taggatgagg
     3241 cattctgagg gattcgaata gaccacttct tcaattgagc tccatagcca gctacttttc
     3301 ctatttcact atttactgac tgctccatac taggaaaaat cagtccagcc ttctcagttt
     3361 ttaaaatata agaataggaa atcagctgga ataagtcctc acggttgatt cctttttcag
     3421 tcaattccag ttttttatat tttgcatcta gaacaatctt tcgttctctg tcataaaaat
     3481 ctggatatac ttttcgtttc ccaacaaaaa atactgaaat tccatccgtc ttatccttat
     3541 ttctgggatg tacaaaacct tttggcaaca aggtgtaaac atactcttcc caaagccagg
     3601 caacatcaaa gagaatacca tagatttttt gatcttgata ccctaaaccg tgcttttctt
     3661 ggtttaggat catcagacaa agttcttgta agtttctgta ctcgtgaaag tatgcatgac
     3721 gtataggttt agattgattt ccccgaataa tcttagcacg atcagctagt ttataagagg
     3781 gcgttacacg cacgatttca gatacgtttt cacgactagt tgagagatta tctagtaccc
     3841 cttgaccaat gcttttctga ttcttaatgt attcaatagt gtgacggacc aactgcatga
     3901 ggggattatc ataggtgaac tctctcgttg tgtaggcaat atttcccgtg aaaggaagat
     3961 ttttcttgag atggtttctt acatcaatca ctcccttaac atgactgtcg ttatgagaaa
     4021 atcgatgata ttccttataa agaccttttc gaatagcggc ttgtagatac ttggggaaga
     4081 gatacatcaa aagttgataa agcctctctt cacgagacaa agcaacatct aaactagtga
     4141 gattgatata aagaaccttg tttaagagat aatgcaaaaa atggtcatta ctctcatcag
     4201 aaaatcgtga ggaaatcgtt aatctttcct gaccatatcc aagaaaacca atcacgttcc
     4261 ctgtcttgat tttctgattg actgtttcaa aaatcttttg gtccttatcc aaatcaggag
     4321 tatgagtcaa atcatttgga aaaatgaaaa tacgttcatc ttgggataga ctttccaagg
     4381 ttctgtctaa aaggagattg cttagtttag gatattcttc aacaaatttt tctttaataa
     4441 tcttgtgttg attatcagta atccgcatca tcgttttcat caccttcatt gtcatcagct
     4501 actgcctgat ctttttgctc attatttgtc agttcaaatg cttttttcaa agtttccaaa
     4561 gtttcaacct catcataaga acctcgcaag tagtcttcta ggagaggctt aatataatca
     4621 gaccagagta attcatagtc aaaatctaca tccttcaact taagaaaata acttggtcca
     4681 atatgataat gactgtttaa ttcctgaaca ttttcgatag cagcgttcaa gtttcttaga
     4741 cgaatttttg cttcttctgc atggatattc agctctttat ccaacatgcc aacttgaccc
     4801 tcggcagtaa cttcaacaaa acgaaaacga cgacgcatag caaaatcaaa ggtatccact
     4861 gaacgatcaa tatcattcat agttccgatg atgtaaacat tttcggggat atagaacttt
     4921 tcatcagttt cgtgtagatt tgcatattgg gtagaaacac ttcctttttc accacgatag
     4981 ccagggtcga tagagaaaaa gagttcgcca aaaatcttag aaatctcccc acgattgatt
     5041 tcatcgatga tgaagacgaa tttcttatca gtatcaattt ctgttgggga aacatagtct
     5101 ttcaaaccaa atctctttct caatgtttct aggacagttt ttccaccact tttgtagtat
     5161 tcttgcttat tataattttt atctttatac aactcgtaaa catatttgct aggtagtgac
     5221 catcctggaa caccactatc ataatttact gacaaatttt gtctactatt aacagataag
     5281 taagatgttt ttgttatata ttctttttct tcagcaacat ttatatattc taagtaagaa
     5341 tcccaagcct catcaaaatt atcttgtcct ccaatcaatt gggtttcttt tgctttctga
     5401 caaaaatctt taaaaatacc gtcctgtagc ctaaactcaa tagctccatc cccatttgat
     5461 actggtctta aaccttctac aaaatccgta taatcatatg atgggtgaaa ttgtacaaat
     5521 ccgatttggt cttcgttgcc atccgttaat tctttggcaa tttctttagc aagataagtc
     5581 tttcctgtgc caggagcacc acgaaggata aggtttggag actttaatag agattgagtt
     5641 aattgatgac gaaaattgtt tgatgaatcc attgtactat tgtcttcctt attttccata
     5701 aaaagcttag aatactcaaa aagcatagtt cctaatttcg atggctccaa ctcaggataa
//
LOCUS       DAVAAL010000114         5749 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVAAL010000114 DAVAAL010000000
VERSION     DAVAAL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.115"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            100..1434
                     /gene="pepC"
                     /locus_tag="VQM60_001704"
     CDS             100..1434
                     /gene="pepC"
                     /locus_tag="VQM60_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET1081538.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(1490..2401)
                     /locus_tag="VQM60_001705"
     CDS             complement(1490..2401)
                     /locus_tag="VQM60_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET1081539.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2425..3228)
                     /locus_tag="VQM60_001706"
     CDS             complement(2425..3228)
                     /locus_tag="VQM60_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET1081540.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(3256..4245)
                     /gene="manX"
                     /locus_tag="VQM60_001707"
     CDS             complement(3256..4245)
                     /gene="manX"
                     /locus_tag="VQM60_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET1081541.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(4478..5497)
                     /gene="adhP"
                     /locus_tag="VQM60_001708"
     CDS             complement(4478..5497)
                     /gene="adhP"
                     /locus_tag="VQM60_001708"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET1081542.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         1718 a         1223 c         1220 g         1588 t
ORIGIN      
        1 tcgaaaaagg tgagggacaa tgtcctcgcc ttttatgttt tttagttgct tcctttgtga
       61 aaagagttat aatagactgt agaataaaag gaggaatcta tgaacgcgat tcaagaatca
      121 tttactgata aactatttgc caactatgaa gcaaatgtaa aataccaagc gattgaaaat
      181 gctgccagcc acaacggaat ttttgcagct ctggaacgtc gccaaagcca tgtagacaac
      241 acacctgttt tctcattgga tttaaccaag gacaaggtca ctaaccagaa agcgtctggt
      301 cgttgctgga tgtttgcggc tctcaacacc ttccgccaca aactcatctc gcaatacaaa
      361 ttggagaact ttgagttgtc acaagcccac actttcttct gggataagta tgagaaatca
      421 aactggttct tggagcaagt cattgcgact tcagaccaag aattgactag ccgcaaggtt
      481 agcttcttac tccaaacacc tcaacaagat ggcggtcaat gggatatggt cgtttccctc
      541 tttgaaaaat acggtgtcgt gcctaagtca gtttatcctg agtctgtttc atctagcagc
      601 agtcgtgagc taaatgcgat ccttaataaa ttgcttcgtc aagatgctca aatcttgcgt
      661 gatttgcttg tttctggtgc agatcaagtg actgttcaag ctaagaaaga agacctcttg
      721 caagaaatct ttaactttct tgctatgtca ttaggacttc caccacgcaa gtttgacttt
      781 gcttatcgcg ataaagataa caactacaaa agtgaaaaag gaatcacacc acaagagttc
      841 tacaagaaat atgtcaatct tcctttagaa gactacgttt ctgttatcaa tgctccaact
      901 gctgataaac cttacggaaa atcttacaca gttgagatgt tggggaatgt ggttggtagc
      961 cgtgcagttc gttacatcaa cgttccaatg gagcgcttga aagaattggc gattgcccaa
     1021 atgcaagcag gtgagactgt ttggtttggt tctgatgtcg gccagctcag taaccgtaag
     1081 gctggaatcc ttgcgacaga tgtttatgac tttgaatcaa gcatggacat taaacttact
     1141 caagacaagg ctggacgttt ggactatagc gaaagcttga tgacccacgc catggtcttg
     1201 acaggtgttg acttggacga aaatggtaaa tcaaccaagt ggaaggttga aaactcatgg
     1261 ggagacaagg tcggtacaga tggttacttt gttgcctcag acgcttggat ggacgaatac
     1321 acataccaaa tcgttgttcg taaggaattg ctgacagcag aagaacaagc tgcctatgga
     1381 gcagaaccaa ttgtacttgc accatgggat ccaatgggag ccttggctaa ataaaagcat
     1441 agaaaaaagg aatcagattt tagaacctgg ttccttttta gttgcttgat tacatgatgt
     1501 gaagaacatg tgccacaata cccactgcga agagggcaag gataatagtg attggagata
     1561 cttttttctt aagtaagtac atgcaaagta aagtaaggag tagtcctgat aatccaggaa
     1621 tcaacatatc caagttttgt tggaaagtag taactttttc aggagtttga gacaatcctt
     1681 gtcctacttg tgcgaatgct tcttggatac ctttagaccc ttctggcaat ttatcccaat
     1741 ggatataagc cttttcatct agttgaactt tagaaacatc gaaagcaaat ttaatattta
     1801 cccagcgttg aacaaggaca gcaagaatga acatcccaag gatagaagct cctttagtga
     1861 tatcttgaag gataccacca gacatatctt tagtgatttc tgatccagcc ttgtatccaa
     1921 tctcttgaac ataccacaag aatgacatac gaatcaagtt ccatgcaaca aagaagagga
     1981 gtggccccaa gatattgcca gtaagggcaa gtgaagcacc gagagatcca aggattgggc
     2041 gtactgtaaa ccagaatact gggtcaccga taccagcaag aggtcccatc ataccgattt
     2101 taaccccttg gatagcagcg tcatcgattt ccacaccgtt agcacgttct tcttcaagcg
     2161 caagagtaac ccccatgact ggagcagcta cgtatggatg agtgttgaag aactcaaggt
     2221 gacgctcaag agcagcgatt tgatcttctt ttttagtata gagttttttg atagctggaa
     2281 tgagtgtata agcccagccc aagttttgca tccgttcaaa gttccaagac ccttgtaaga
     2341 aggttgaacg ccaccaaact tttttacgat ctgatttagt taattgaagt ttttcagtca
     2401 tgatgttttc agtcctttct tatcttagta gtcttctagg atatcgccga ttgggtcgtt
     2461 agaagttgcg gctcctccgc caccatttcc accagtttta gaaaggtgaa ggtagataag
     2521 agcgatagca acaccgatag caccgaatcc gattagagta atatctgaca cagcagcgag
     2581 aacgaaacca agagcgaaga atggccatac ttcacgagtt gccatcatgt tgataaccat
     2641 ggcgtaacca acggcaacga ccataccacc accgatagcc ataccatctt tgagccagtc
     2701 tggcatggca ctaaggatac tttgtacagt ttcagttggt accataagga gaagagctgc
     2761 aggaagcgcg atacgaagtc cttggaagag tagcgcaatg aaatgcgcac gctccacagc
     2821 gccgaagtca ccttttttag cggcagcatc tgcagtatga accaaaccaa ctgaaattgt
     2881 acgaacaatc attgtcaaga aaagtccagc tacggcaaga gggatagcaa ccgcttgggc
     2941 aacaccgata ccagtcttgg taaagtcacc accaagaacc atgataatgg cagcagcgac
     3001 agaagcaagt gcagcatcag gagcgatagc agcaccgata tttgaccaac caagggcaat
     3061 catttgaagc gatccaccga ggataatccc tgcttccaag tgacctgtta caagcccaat
     3121 aagggtacag gctacaagtg gttggtgaaa ttggaactgg tcgaggatgc cttcaagacc
     3181 tgcaaagaag gctacaacga ctactaaaac catagaaata atagacatgt ttaaaatcct
     3241 ttcataaata atggcttatt tgacattggc tttgttaatc aagtcaaaca aatctttttt
     3301 agaatcattt ggtactttac ggacatcaaa ttcaacaccc aagtcacgca ttttttcaaa
     3361 tgtagcaaca tcttctttgt ccatagacaa aacggtattg accaatgttt tacctgttga
     3421 gtgagccata gaaccaacgt taagagtctt gattggcacg ccgccttcga tggcacgaag
     3481 ggcatcttga ggtgtttcaa acaagataag ggcatgtgtt tctccaaaac gtgggtcttt
     3541 tgaaatctca atcagttttt gaattggaac cacgttagcc ttgacattac ctggagctgc
     3601 ttgtttaatc aattctttac gaaggtcgtc tttagccacg ttatctgaag caacgatgat
     3661 acgatttgct tttgaatctg gagtccaagc agttgcaacc tgaccgtgaa gtagacgtgt
     3721 gtcaagacgg gcaagattga ttttcaattt accgtctccg ataacagttc cttctgggat
     3781 agcagtttgg gcaactggag cagctgcagc gcttgcaact tcttcaactg gatttagctc
     3841 ttctggaaga gctttgatgc catctttggc ttctttaatg atattagcag cgactttttc
     3901 tacacctgca gcagcgtcca tgaggcgctc tgtgtaggct tgaatcaaca tcggtaagtt
     3961 aagtcctgtg atgatggcaa acttacgctc aggattttct cccatcacgc gactagcttg
     4021 gttaaatgga gaaccactcc aaaggtcagc caaaactaga acctcatctt ctgcgtcaaa
     4081 tgcagcaaca gcgttattaa acttagcgta tagatcatca ggaccttcat ttggcataaa
     4141 ggtcacaact tgaacctttt cttgttcacc aaagatcata gatcctgact gatgaatacc
     4201 agcagcaaat tcgccgtggc tcgcaataat gattccgata ctcattattg tcattcctcc
     4261 ttttttgttt tagttttcat ttaagaaaac tttaagactg tttaagtata aaccgttttc
     4321 ataaaaaaag caactattta tcataaaaaa ttaaaaaatt tttatttttg tatgttgata
     4381 tgttgagttt ttgaggagta atcttgaatt cttatttaaa atttaatata aaaaagttgg
     4441 aagaagcttc caacttttaa aataggtttc tattaaatta gtgggtgaag tcgagtacca
     4501 tacgtccttg gatttggcct ttttccattt cgtcgaaaat ggcaacagca tcttctactg
     4561 gacgtttttg aacaactggg actaccagac cttctgcacc aaattggaag gcttcttcta
     4621 agtctttacg agttccgaca agagaaccga tgacttggat tccatcgagg actgttttaa
     4681 cgatgctgag ttccatcatt tcagaaggaa gaccaacagc gacgacgcga ccaccagcac
     4741 gaatggagtc aacagcctgg ttgaaggcaa ctttagacac agcagttacg acagctgaat
     4801 gagctcctcc atcagttttt tctttaatga gtccagctac atcttcaact tcgaggccgt
     4861 taatcacaat gtcagcgcct acttcttttg caagggtaag tttgtcattg ttgatatcga
     4921 ctgcgataac atgagcattg aatacttttt tagcgtattg aacagcgagg ttaccaagtc
     4981 caccagcacc gtaaagaaca acccattggc ctggttcaac ttttgcttct ttgatagctt
     5041 tataggttgt tactccagca catgtgatag aagaagcttg ggctggatca agtccgtcag
     5101 gaactttgac agcatagtca gcagttacga tacattgttc agccatacca ccgtctactg
     5161 agtagccagc atttttcact gtacggcaaa gggtttcgcg accagttgta cagtattcgc
     5221 aagtgccaca tccttcaaag aaccaagcaa cgctgacgcg gtcaccgact ttaaggcttt
     5281 tcacatctgg agcaatctct ttaacgatac cgataccttc gtgcccaaga acacgtcctg
     5341 ggacttgacc aaagtcacca tgagcaacgt ggaggtcggt gtggcaaacg ccacagtatt
     5401 caacttctac aagtgcttcc ccagtttcaa gtggacggag tactttttct tcaatagcaa
     5461 caccagtgct ttctggattt acaacaacag ctttcatagc tatcctcctt gatattgacc
     5521 gagaacaggg acaacgggac tggattgatt ttttgtcaac agagtcgagt gtgacgagct
     5581 ctcttgtatt tatatgtgaa gtatatcaca aaagaaagcc ttttccaagg ttttggagac
     5641 aaaaagtaga acaatcgatt aactggttga tgagactgtg aaaagagcac aaagaccagc
     5701 ttgcaagctg gtcatactct tcgaaaatct cttcaaacca cgtcagctt
//
LOCUS       DAVAAL010000115         5706 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVAAL010000115 DAVAAL010000000
VERSION     DAVAAL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.116"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            89..1264
                     /gene="mutY"
                     /locus_tag="VQM60_001709"
     CDS             89..1264
                     /gene="mutY"
                     /locus_tag="VQM60_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET1081543.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1319..2023
                     /locus_tag="VQM60_001710"
     CDS             1319..2023
                     /locus_tag="VQM60_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1081544.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2016..3365
                     /gene="vicK"
                     /locus_tag="VQM60_001711"
     CDS             2016..3365
                     /gene="vicK"
                     /locus_tag="VQM60_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET1081545.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3367..4176
                     /locus_tag="VQM60_001712"
     CDS             3367..4176
                     /locus_tag="VQM60_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET1081546.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4267..4509
                     /locus_tag="VQM60_001713"
     CDS             4267..4509
                     /locus_tag="VQM60_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET1081547.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4511..4765
                     /locus_tag="VQM60_001714"
     CDS             4511..4765
                     /locus_tag="VQM60_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET1081548.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQINVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4812..5585
                     /locus_tag="VQM60_001715"
     CDS             4812..5585
                     /locus_tag="VQM60_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET1081549.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENVFDLLAILPLNAIFTVFRLGRIFRLVKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIILVGSSILSVVEEKSFS
                     DSLWWTLVTVTTVGYGDIVPVSLLGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLQDELSSPRILLEKQSKKIEELHKMIQDLIEKT"
BASE COUNT         1785 a          935 c         1268 g         1718 t
ORIGIN      
        1 agtagataga aaagttatat actatctatt gaaaatatta taaatcagat acaaagacag
       61 gtgaaacctt ctgtgttata ctagagatat gttagatttg aaagaatacg gtatcgtcat
      121 gtggccggag gagaaagtca tttctttccg tgagaaactt ctcgcttggt atgatgaaaa
      181 caaaagagat ttgccttgga ggagaagtaa aaatccttat cacatctggg tatctgaaat
      241 catgcttcag cagaccaggg tggatacagt tatcccttac tacgaaagat ttttggactg
      301 gtttccaact gtcgaaagtc tggcaactgc gcctgaggag agtttactga aagcttggga
      361 gggcttgggt tattattctc gagttcgcaa tatgcaggct gcagcccagc aaattatgac
      421 tgactttggt ggccaatttc caaataccta tgaaggaatt tccagcttga aagggattgg
      481 accttacaca gcaggagcca tttccagtat tgcttttaac ttgcctgagc cagctgtaga
      541 tggtaatgtc atgcgggtct tggcgcgtct gtttgaagtc aaccacgata ttgggattcc
      601 aagtaatcga aaaatttttc aggcaatgat ggagatcttg attaatccag atcgtccggg
      661 tgattttaat caagccttga tggacttagg gtctgatatt gagtctcctg taaatcccag
      721 acctgaagaa agcccagtca aggactttag tgcggcatat cagaatggaa ccatggaccg
      781 ttatccaatt aaatctccta agaaaaagcc ggttcctatt tatcttaaag ccttagttgt
      841 gaaaaattct caaggacaat ttttacttga aaaaaatgaa agcgaaaagc tcttggcagg
      901 tttttggcat ttccccttta tagaagttga taacttttcg caagaagagc agtttgacct
      961 ctttcatcag gttgcggaag aaagtgtgaa ctctggcccc agtccagaag agagtttcca
     1021 gcaggactat gacctagatg ttgattggct tgatgtttgt tttgatacag tccagcatgt
     1081 ttttagtcat cgaaaatggc atgtacagat tgtagcaggt caggtgagtg acttccatga
     1141 tttttcagat agggaagttc gttggctttc accagaagaa ttcaagaatt acccacttgc
     1201 caaaccccaa caaaaaatct ggcaggctta tgcacaagcc aacttagaca gtagccaaga
     1261 ctagctattg tgtcttctga ctattttttg gtataatagt agagaaaaag gtgaacatat
     1321 gaaaaaaata ctaattgtag atgatgagaa accaatctcg gatattatca agtttaatat
     1381 gaccaaggaa ggttacgaag ttgtaactgc ttttaatggt cgtgaagcgc tagagcaatt
     1441 tgaagcagag caaccagata ttattattct ggatttgatg cttccagaaa ttgatggttt
     1501 agaagttgct aagaccattc gtaagacaag cagtgtgccc attcttatgc tttcagccaa
     1561 agatagtgaa tttgataagg ttatcggttt ggaacttggg gcagatgact atgtaacgaa
     1621 acccttctcc aatcgtgagt tgcaggcgcg tgttaaagct cttctgcgtc gttctcaacc
     1681 tatgccagta gatggtcagg aagcagatag taaacctcaa cctatccaaa ttggggattt
     1741 agaaattgtt ccagacgcct acgtggctaa aaaatatggc gaagaactag acttaaccca
     1801 tcgtgaattt gagcttttgt atcatttagc atcgcataca ggtcaagtca tcacgcgcga
     1861 acacttgctt gagactgtct ggggttatga ctattttggt gatgtccgta cagttgatgt
     1921 gactgtacga cgtctgcgtg agaagattga agatacgccc agccgaccag agtatatctt
     1981 gacgcgccgt ggtgtagggt attacatgag aaataatgct tgatttactg aaacaaacca
     2041 tttttaccag agattttatc tttatcctga ttttgttagg tttcatcctt gttgtgaccc
     2101 tcttattact ggaaaataga cgtgataata ttcagttgaa gcaaatcaat caaaaggtta
     2161 aagatttgat tgcaggagat tattccaagg ttcttgatat gcaaggtggg tctgaaatca
     2221 ccaatattac caataatttg aatgacttgt cggaggttat tcgtctcact caggaaaatc
     2281 tagaacaaga gagtaagagg ctaaatagta ttctgtttta tatgacagat ggggttcttg
     2341 cgaccaaccg tcggggtcag attatcatga ttaacgatac agccaagaag caactggggt
     2401 tggttaagga agatgttctg aatagaagca ttttggaatt gctcaagata gaagaaaact
     2461 atgaattgcg tgatttgatt acccaaagtc cagaattgtt gctagattcc caagatatca
     2521 atggcgaata tttgaacctt cgagttcgct ttgccttgat acgtcgagag tctggcttta
     2581 tttcaggttt ggtggctgtt ttgcatgata cgacggagca ggagaaggaa gaacgcgaac
     2641 gaagactctt tgtttccaat gttagccatg agttacggac tcctctgact agcgtaaaat
     2701 cctatcttga agccttggat gagggggctt tgtgtgaaac tgtagcacca gactttatca
     2761 aggtttctct tgatgagacc aaccgtatga tgcgcatggt gacggatctc ctccatcttt
     2821 cacgtattga taatgctacc agtcacctag atgtggaact gattaacttc actgctttta
     2881 ttacctttat cctcaatcgt tttgacaaga tgaaaggaca ggaaaaggag aaaaaatatg
     2941 agttggtgag agattatccc atcaattcta tctggatgga aattgataca gataagatga
     3001 cgcaggttgt cgacaatatt ttaaataatg ctattaagta ttcgccagat gggggtaaaa
     3061 tcactgtcag aatgaagaca actgaagacc agatgatttt atccatttct gaccacggtt
     3121 tggggattcc taagcaggat ttaccacgta tctttgaccg tttctatcgt gtggatcgtg
     3181 ctagaagtcg tgcacaaggt ggtacaggtc taggactgtc tatcgctaaa gaaattatca
     3241 aacaacataa gggctttatt tgggccaaga gtgaatacgg caagggttca acctttacca
     3301 ttgtgctccc ttatgataag gatgcagtga aagaagaagt atgggaggat gaagtagaag
     3361 actagaatga gtgaaatagg ctttaaatac agtattttag cgtcgggttc cagtggaaat
     3421 tctttttatc tggaaacctc aaaaaagaag cttttagtag atgcaggctt gtctggcaag
     3481 aaaattacca gtctgctagc tgaaattaac cgtaagccag aagacctgga tgccatcttg
     3541 attacccatg agcattcaga tcatatccat ggagtaggcg ttttggctcg caagtatggt
     3601 atggatcttt atgccaatga aaagacctgg caagctatgg aaaatagtaa atatcttggc
     3661 aaggtggatt cttcgcaaaa gcatattttt gaaatgggta aaaccaaaac ctttggagat
     3721 atcgacatcg agagttttgg tgtaagccat gatgcagtcg caccgcagtt ctatcgcttt
     3781 atgaaggatg ataagagttt tgtccttttg acagatacag gttatgtcag tgaccgtatg
     3841 gcgggcattg tcgaaaatgc ggatggctat cttatcgagg ccaaccatga tgtagagatt
     3901 ttgcgatcag gttcttacgc ttggcgactc aaacaacgaa tcctatctga ccttggtcac
     3961 ctttctaacg aggacggtgc tgaagctatg attcggacgc taggaaatcg tactaagaag
     4021 atttaccttg ggcatttatc taaggaaaac aatatcaagg aactggctca tatgaccatg
     4081 gtcaatcagc tggctcaagc tgatctggga gtcggagtag actttaaggt ttatgatacc
     4141 tcaccagata ccgcaacacc attgacagag atatagaaag aacgctgaga aggtgttctt
     4201 tttatattga ctgaacacct aaaaagtaat acaatggtgt taccattaaa aaagggagca
     4261 caaaagatga ctactattac attaaaagtt tctgaagctg ataaaacatt tatgaaagca
     4321 atggctaagt ttgaaggagt ttccctttcg gaacttattc gcaccaaaac tcttgaagcc
     4381 ctagaagatg aatacgatgc tcgtgtggca gatttagcct atcaagagta tttagaagac
     4441 ttggaaaaag gagttgaacc cattacttgg gaagaaatga tgcatgattt aggcttgaag
     4501 gatgaataat ttgtataaat tagttccaac aagacgtttc atcaagcaat tgaaaaaatt
     4561 ggaccgttat acgcagaagc taattacaaa ctatttacaa atcaatgttt tggaagaccc
     4621 aagacgacac ggaaaggctt tggttggtaa tcgcgttggt caatggcgct atagaattgg
     4681 taattatcga gttatcgtac aaattgtaga tgatgaatta gttgttgcta ctctagaagt
     4741 tggtcatcgg agagatattt attgaattaa agctgagtaa taacaatata gaaagtaagg
     4801 agagtaaaaa tttgaagaaa aaatggtttt ttgcagatta ttatgataca acaattattt
     4861 tacttgcttt aatctctgtt attctggtat tacttggatt tgctgagatg attgatttgg
     4921 acaatccgcc ttatagtatt attgatttag taatctgggg tgtttttgta attgattata
     4981 gttggcgctt ttttattact aaaagaaaat ggcgctttat tcttgaaaat gtttttgatt
     5041 tattggctat tctcccttta aatgctattt tcacagtttt tcgattaggg cgtatctttc
     5101 gtttagttaa actaacaaaa ttactgaaac tgactcgttt acttagaata attggactta
     5161 caggtaaatt agaaagaaaa attagtagat tcttacggac aaatggcctg atttatatct
     5221 tatatgttaa tatctttatt attctagtag gaagttcaat tttatctgta gttgaagaaa
     5281 aatccttctc agatagtctt tggtggactc ttgtgacagt aactactgtt ggttatggtg
     5341 atattgttcc agtttccctt ttggggaaat ggctagcagt tttactgatg cttgtcggta
     5401 ttggaacaat agggatgtta acaagtgctt tgacgaactt ttttgtaaag gataatccag
     5461 atgaacagat aaaacttgat aaactccaag atgaattatc tagtccgaga atattactag
     5521 agaaacaatc taaaaagata gaagaactac ataaaatgat acaagatttg attgaaaaaa
     5581 cataataaaa ataaagatga gaaaaagttt gaactgagga gaaatcaagt aagttttgac
     5641 acaatagaac gcatattatc aggtataaaa acttgatact atgcgtttta ttgtgggaag
     5701 atgtat
//
LOCUS       DAVAAL010000116         5654 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVAAL010000116 DAVAAL010000000
VERSION     DAVAAL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.117"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            190..504
                     /locus_tag="VQM60_001716"
     CDS             190..504
                     /locus_tag="VQM60_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081550.1"
                     /translation="MWVLGFILFLIFFYSNDSKKIKKLEKKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            514..720
                     /locus_tag="VQM60_001717"
     CDS             514..720
                     /locus_tag="VQM60_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1081551.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            720..917
                     /locus_tag="VQM60_001718"
     CDS             720..917
                     /locus_tag="VQM60_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081552.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            920..1654
                     /locus_tag="VQM60_001719"
     CDS             920..1654
                     /locus_tag="VQM60_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET1081553.1"
                     /translation="MKKKRRLLFLMSIVFGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            1666..2307
                     /locus_tag="VQM60_001720"
     CDS             1666..2307
                     /locus_tag="VQM60_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET1081554.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLVLGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2325..2555
                     /locus_tag="VQM60_001721"
     CDS             2325..2555
                     /locus_tag="VQM60_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081555.1"
                     /translation="MKRVILVAVIQAVVLFFIIGGLAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            2591..2671
                     /locus_tag="VQM60_001722"
                     /pseudo
     CDS             2591..2671
                     /locus_tag="VQM60_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2907..4670
                     /gene="aspS"
                     /locus_tag="VQM60_001723"
     CDS             2907..4670
                     /gene="aspS"
                     /locus_tag="VQM60_001723"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET1081556.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRVKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
     gene            4648..5589
                     /locus_tag="VQM60_001724"
     CDS             4648..5589
                     /locus_tag="VQM60_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET1081557.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
BASE COUNT         1599 a         1032 c         1260 g         1763 t
ORIGIN      
        1 gaagctgacg tggtttgaag atattttaga agagtattaa tgatagataa agtggtcagg
       61 aacttactcc tgaccttttt cttttgcaaa atgacaaaaa tcataaactt tttgacaact
      121 atacttgtca aaaagaaaaa gatgtgttac aatgtaagca agttaagaga aaagaagaaa
      181 ggagctatga tgtgggtact agggtttata ctatttctga ttttctttta ttctaatgat
      241 tctaaaaaaa tcaagaaact agagaagaaa atcaaaagac ttgagcgaaa agagaaagga
      301 aatgcagaaa tgtcgagatt attacaagaa atgattggaa aggaaccaat tataacggga
      361 gtgtatattg ggccagataa ctgggaagtt gtggatgttg atgaggaatg ggtaaagcta
      421 cgacgtgtag ataatacggg aaaagaaaaa ttcaagttgc aacgtattga ggatatccaa
      481 accgttgaat ttgacggaga gtaggtgtgt aacatgcaac taaaaaatcg tctaaaagag
      541 cttcgagctc gcgatggtct caatcaaacc gacctagcca aactggcagg ggtttccaga
      601 cagaccatta gcctactaga acgggatgag tacaccccat ccattatcat tgccctgaaa
      661 atatctcaaa ttttcaacga aacagtcgaa tcggtatttc gtttggagga ggacgagtga
      721 tgaacaagta taaagtgatt tattatgtag tggtcatagc tttattagtc agcgtatttc
      781 tactgattgg gatggaccta agctggttta atccctatca aagcgaccaa tttgtttggg
      841 tctactttgc tctcatccca gtaattgaat ggattgaaaa gaaatccaaa aatctagcaa
      901 gtgaaaaagg agaatgaata tgaaaaagaa acgtagattg ttatttttga tgtctattgt
      961 ctttgggggg ttcttgggca tgtttgtagg gatgtttaag gcacgtgtcg aatcccacga
     1021 aattatttta gatgtaaaag ccttgatgcc atggatatca gctatttgtt tactgatagg
     1081 tttcattagt atgtttttga ctttcaattt cttaaagaaa agtagaaaat ttcattcctt
     1141 gtatcaagag gaaatggatg acgatctgaa tgaaacctat tatgtgcaaa tgtatcggaa
     1201 tcttgagttt ggaaccattg cttttaatat tacaggtgta gcgattccat tggctatttt
     1261 tatttcatta agtgaggtga ttatattgca tacaaaccct caaacatttt tcctttcttt
     1321 cttactcttt gtggtattct tagtcgctca aaaatctctt tttaaaacca ttgcgattgt
     1381 tcgtcagttt gatttggaat ttttcgctac accaaaggat gtcttgaact atataaattc
     1441 ttatgatgaa ggggagcgtc aggctaattt ggaacagagt tttcgaattt tattccaatt
     1501 acaccaatat gtcttaccag ccttatatat ttttcttatt atcatttctt tcttgacagg
     1561 agagattcag ttactagctt tcttgcttgt aggagccatc catgtttata tcaatgtgat
     1621 gcagttacct atggtaaaac gttatttcaa ataaaggagt tatctatgaa actacttaaa
     1681 aaccttggct ggtttcttct agcccttcta tcctttttct ttatctatgg ctttattcag
     1741 gggctcgcga ctgcgtcgct tgttttaggc gcttcaccct atgctgttac tctactctat
     1801 gtggtcttgg ctggagtata tgtgtacggc atttacaaat ggtatcagaa agctcctgtt
     1861 tatattgaga agagcggctt taaccgattt atttggcttc ctgccttggt ttggttctta
     1921 tctctggtcg ttcaattctt cttgccagat gatccttcag taaatcagca aatagcgaca
     1981 gacttgacct tgtctcaacc acttttctca ttctttgctg tggttatttt tgctcctttg
     2041 acggaagaga ttgtttttag agggatgtta gcacgctatc tctttcctaa gcaggacaat
     2101 agtaaacgaa ccctgatttt tcttctggta tctagtcttc tatttgcctt gattcatttt
     2161 ccaggtgatg tgcaacaatt ttttgtctat tttagccttg gttttagttt gggtttggct
     2221 tacattagca gaaaaggtct ggtctacagt atttctcttc acgctttgaa taatttagtc
     2281 ggctttttga tgattctcat gctataatag agtcaggagg tcacatgaaa cgagtaattt
     2341 tagtagcagt gattcaggca gtcgttctat ttttcatcat tggagggcta gcttatgcct
     2401 tcaaaggcga tttcttctat aactatctag cagttgtctt tgctcctatt gcaggtgtac
     2461 tgcgttttgg gacggcttac ataacggaaa ttgtcttgcc tcgaaaggca gccgaaatcg
     2521 ctgaaaagcg taaagcaggc aaaaattcaa aataaaagag tccggtgaag ttcatcggat
     2581 ttttgtatta tactcaatga aaatcaaaga gcaaactagg aagctaaccg caggttgctc
     2641 aaaacactgt tttgaggttg tggatgaaac tgacgaagtc agctcaaaac atggttttga
     2701 ggttgtagat gaaactgacg gagtcagtaa ccatacctac ggtaaggcga cgctgacgtg
     2761 gtttgaagag attttcgaag agtattagcg ctaattttta gggctttcat ctgactttta
     2821 aagactggca aacttttctg atgattttca tgaagagcct gcgcttttat ggtaaaatag
     2881 taacagaata aaagaggaga gaaataatga aacgtagtat gtatgctggt cgtgttcgtg
     2941 aggaacacat cggacaagaa ataaccttga aaggatgggt tggccgtcgt cgtgaccttg
     3001 gtggtttgat ctttatcgat cttcgtgacc gtgaaggaat catgcagttg gttatcaacc
     3061 ctgaaaaagt atctgcagag gttatggcaa cagctgaaag ccttcgtagc gaatttgtta
     3121 ttgaggtgac tggtcaggtc gctgcgcgtg agcaagccaa tgataagttg ccaactggtg
     3181 cggttgagtt aaacgtgaca gctcttattg tgcttaacac agctaagaca acaccatttg
     3241 agattaagga tggcattgag gcaaatgacg atacacgttt gcgttaccgt taccttgacc
     3301 ttcgtcgtcc agaaatgttg gaaaatctta aacttcgtgc caaggtgacc cactctatcc
     3361 gcaactactt ggatgagttg gagtttatcg acgtggagac accattcctt tctaagtcaa
     3421 cgcctgaagg ggcgcgtgat tatttagtgc cgtctcgtgt taataagggg catttttacg
     3481 ctcttcctca aagtccacaa atcacgaaac agctcttgat gaatgctggt tttgaccgtt
     3541 actaccaaat cgttaaatgt ttccgtgacg aagacttgcg tggagaccgc cagcctgaat
     3601 ttactcaggt cgacttggaa acgtccttcc ttactgagca agaaatccaa gatattacag
     3661 aaggcttgat cgcgcgcgtg atgaaggaaa caaaaggcat cgaagtaacg ctaccgttcc
     3721 ctcgtgtgaa atacgatgat gctatggctc tttacggttc tgacaagcca gatactcgtt
     3781 ttgacatgtt gcttcaggac ttgacagaag tggtcaaagg tgtagacttt aaagtctttt
     3841 cagaagcacc tgctgtaaaa gcgattgtgg tcaaaggagc tgcggacaac tattcacgta
     3901 aagacatcga caagatgacg gaagtagcca aacagtatgg tgccaaaggt cttgcttggg
     3961 ttaaggtggt tgatggagaa ttaaacggac cagttgccaa gttcttgact ggtatccaag
     4021 aagaattgac aacagcgctt gctcttgaag ataaggactt ggttctcttt gtggcggata
     4081 cgcttgaagt ggctaatgca acactgggtg cccttcgtgg acgtattgct aaagagcttg
     4141 gcttgattga taatgataag ttcaacttcc tttgggtggt tgactggccg atgtttgaat
     4201 ggtctgaaga agaaggccgc tacatgagcg cccaccatcc tttcaccctt ccacaggaag
     4261 agactgctca cgaattagaa ggtgatttgg ctaaggttcg tgccattgct tacgatatcg
     4321 tcttgaacgg ttatgagctt ggtggtggta gccttcgtat caaccaaaaa gaccttcaag
     4381 aacgcatgtt caaggctctt ggtttctcag ctgaagaagc caatgaccag tttggtttcc
     4441 ttcttgaagc catggactat ggtttcccac cacacggtgg tttggctatc gggcttgacc
     4501 gttttgtcat gttgcttgct ggagaagaaa atatccgtga agtcattgcc tttcctaaga
     4561 acaacaaggc aactgaccca atgacacaag ctccatcaac agtcgctctc aaacaactag
     4621 aggaactcag cttacaagta gaagaagatg aaacaagcaa aacgaattaa gcggtggcgc
     4681 tattatctgc gccgctttgc ttatcagata aaaattttac gtgtcttaca aagtatctct
     4741 cgagaaaagt atgatgagaa gatttcggcc tctctggtct atggtttttt atcagcagta
     4801 gcagttaatt tctttttcca accagggcat gtgtattcga gtggtgcaac aggtctggca
     4861 cagattatct ctgccttgag taatcactgg tttggttttc atattccgat ttcgctaagc
     4921 ttctacgcca ttaacttccc tttgatggtc ttagcttggt atcagattgg ccataagttc
     4981 accgtcttta cctttatcac ggtatctatg agttccttct ttatccagtt tgtccctgtg
     5041 gcaaccttga cggaggatcc cattatcaat tccctttttg ggggtgttgt tatgggtttg
     5101 gggattggtt ttgctcttcg aaacaatatc tccagtggtg ggacggatat cgtcagcctg
     5161 actattcgca agaaaacggg taagaatgtc ggtagtattt ctttcttggt aaatggaact
     5221 atcatgctga tagcaggttt gacctttggt tggaaatacg ctctttattc tatgattacc
     5281 atctttgtct ctagccgtgt gacagacgca gtctttacta agcaaaagcg tatgcaggcc
     5341 atgattgtga caaatcatcc agagaaggta attgaaaaaa tccataaaaa attgcaccgc
     5401 ggagcaacca tgatccacga tgcagaagga acctataatc acgagagaaa ggcagtttta
     5461 atcactgtca ttacacgtgc agagtttaat gaatttaaac agattatgac acaagtggat
     5521 ccaagctcct ttgtctctgt ctcggaaaat gttcatattc taggaagatt tgttgagata
     5581 gataattagt gattaatact cttcgaaaat ctcttcaaac cacgtcagct tcaccttgcc
     5641 gtatatatgt tact
//
LOCUS       DAVAAL010000117         5513 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVAAL010000117 DAVAAL010000000
VERSION     DAVAAL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.118"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            94..1848
                     /locus_tag="VQM60_001725"
     CDS             94..1848
                     /locus_tag="VQM60_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081558.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     TLLALTIIGGLILGAMMGEQEFMKIYQEALSKLSAETVEYVRGMQVVKIFKANVESFK
                     IFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHSNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1832..3553
                     /locus_tag="VQM60_001726"
     CDS             1832..3553
                     /locus_tag="VQM60_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081559.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMPAGNVKMGL
                     AVIIPSILNFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKEAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            3564..4148
                     /locus_tag="VQM60_001727"
     CDS             3564..4148
                     /locus_tag="VQM60_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET1081560.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLIIYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            4148..4843
                     /locus_tag="VQM60_001728"
     CDS             4148..4843
                     /locus_tag="VQM60_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET1081561.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            4831..>5513
                     /locus_tag="VQM60_001729"
     CDS             4831..>5513
                     /locus_tag="VQM60_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081562.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFL"
BASE COUNT         1977 a          718 c         1036 g         1782 t
ORIGIN      
        1 ttataacaga tgtataatag aaaagcaatg atagatatta tcaattaagc gaatttatat
       61 ctaaaaggga tattaaagaa aggagatatg cttatgaaga tttacaaaaa actatttgct
      121 tatgtccaag ataagaaata tcttggggtt ttggccataa ttttttctgc tatatctgct
      181 ctacttacag tatatggata ttatttaatc tacaaatttc tagataagtt aataattaat
      241 tcaaatttat ccggtgcaga gagcatagca ttaaaatctg ttattacact aacaagtgga
      301 gcgatatttt attttgtctc aggaatgttt tcacatatct tgggattcag gcttgaaaca
      361 aatttaagaa aaaggggaat cgatggtctg gaaaaagcaa gttttaggtt ctttgactta
      421 aatccatctg gtcaaataag aaagatcata gatgataatg cagcacaaac tcatcaggtg
      481 gtagctcaca tgattcccga tagctctcag gcaatagtca cacctatact tgtccttgta
      541 ctaggcttta tagtaagtat aagggttggt ataactttgc ttgctcttac tataattggt
      601 ggcttaattt taggggcgat gatgggcgag caagaattta tgaagatata ccaagaagct
      661 ctatctaaac taagtgctga aactgttgag tacgtgagag gaatgcaagt tgtaaaaata
      721 tttaaagcaa atgtagagtc ttttaaaatc ttttataagg cgataaaaga ttactcaaag
      781 tatgcttatg attattccct atcttgtaaa aggccttatg ttttgtatca atggttattt
      841 tttggactga ttgcaatttt aattattcct atagtttatt ttatgactag cttagctagc
      901 gcaaaggtga ttttacttga gcttatcatg attttatttt tatcaggagt tctctttgtt
      961 tcattcatga gaatgatgtg gtactccatg tatatttctc aaggaaatta tgcagtagat
     1021 actttagagg cgctttacga agatatgcaa aaagacaaat tagtgcatag taatgtcaat
     1081 aattttaaaa actataatat agaatttgag aatgttagct ttgcttataa tgataaagct
     1141 gtcattgaaa atttatcctt taatttagaa gaaggaaagt cctacgcact tgtcggttca
     1201 tctggatcag gcaaatcaac agtagcaaaa cttatatcag gtttttacaa tgttaataaa
     1261 ggaagcataa agataggcgg gatagcaata agtgaatatt ctgacgaagc cttaattaaa
     1321 gccatttcct ttgtttttca agattcaaaa ttattcaaga agagcattta tgataatgta
     1381 gctttagcta ataaagatgc gacgaaagat gacgttatga gagccttaaa attagcagga
     1441 tgcgatttaa tattagacaa attcccagaa agagaaaata caatcatagg ctcaaaaggt
     1501 gtttatttat ccggtggaga aaaacaaaga attgcaattg ctagagcaat tttaaaggat
     1561 tccaaaatta ttattatgga tgaagcatca gcatctattg acccagataa cgagtttgaa
     1621 ttgcaaaaag cttttaaaaa tcttatgaag gataaaacag ttatcatgat tgcacacagg
     1681 ctatctacaa ttaaagacct tgatgaaatt attgtcatgg atagtggaaa aattatagaa
     1741 agagggtctg acaaagaatt aatgtcaaaa gatacaaggt ataagagcct gcaagagatg
     1801 tttaacagtg cgaatgaatg gagggtttca aatgaaagag ttttataaaa aaagatttgc
     1861 tcttacagat ggaggagcaa gaaatttaag taaagcaaca ctggcttcat ttttcgttta
     1921 ttgtataaac atgcttcctg ccatattact tatgattttt gctcaggaag ttttggaaaa
     1981 tatgggcaaa agcaatggct tttatatagt attctcagtt ttgactttga tagcaatgta
     2041 tattttgctt tctatcgaat acgataaatt atataacaca acctatcaag aaagtgcaga
     2101 tttaagaata aggacagcgg agaatttatc aaaattacct ctatcttact tttctaaaca
     2161 tgacatttcc gacatttcac aaacaatcat ggctgatatt gaaggcatag agcatgcaat
     2221 gagccactca ataccaaagg tgggcggcat ggtactgttt ttcccattaa tatctgtaat
     2281 gatgccagcg ggcaatgtca agatgggttt agctgtaatt attccatcta ttttaaactt
     2341 tatatttata cctttatcta aaaaatatca ggttaatgga cagaatagat attatgatgt
     2401 cttaagaaaa aactcagaaa gctttcaaga aaatatcgaa atgcaaatgg agattaaagc
     2461 atataattta tcgaaggata ttaaagatga cttatataaa aaaatggaag atagtgagag
     2521 agtacactta aaggcggaag taactacaat tttaactttg tctatatctt caatatttag
     2581 ctttatatct cttgctgttg tgatatttgt cggcgtaaat ctaattatta ataaagagat
     2641 aaattctctc taccttatag gatatttact agctgctatg aagataaaag actctttaga
     2701 tgcatctaaa gagggcttga tggaaatatt ttatttatcg cccaaaatag aaagattaaa
     2761 agaaattcaa aatcaagatt tacaagaagg cgatgactat agcttaaaaa aatttgatat
     2821 tgatctaaaa gatgttgagt ttgcctacaa taaagacgca aaagttttaa acggtgtaag
     2881 ttttaaagct aagcagggag aggtcactgc tttggtaggt gcaagtggct gcggtaaaac
     2941 aactatcttg aaacttatat caagacttta tgattatgac aagggacaaa tcttaattga
     3001 tggcaaagat ataaaggaaa tatcaacaga atcccttttt gataaggtgt ctattgtttt
     3061 ccaagatgtg gttctcttta atcaaagcgt tatggaaaat attagaatcg gtaagcaaga
     3121 tgcaagtgac gaagaggtta aaagagcagc aaaacttgca aattgcacag attttataga
     3181 aaaaatggat aaaggtttcg atacagttat tggtgagaac ggagctgagc tatcaggagg
     3241 agaaagacaa agattatcaa tagccagagc cttcttaaaa gaagcgccga tattgatctt
     3301 agatgagata acagcaagcc ttgatgttaa caacgagaaa aagattcaag agtctttaaa
     3361 taatttagtt aaagataaaa ctgttgtaat catttcacat agaatgaaat ccatagaaaa
     3421 tgcagacaag atagtagttc ttcaaaacgg aagagtagaa agcgaaggta agcatgaaga
     3481 gcttttacaa aaatcaaaaa tttacaaaaa tttaatagaa aagacaaaaa tggcagaaga
     3541 atttatttat taggaggact acaatggata ataaaaaatt aaaagtaaaa gatttagtaa
     3601 gtatcggtgt ttttggcgta atttattttg ccttcatgtt tggagttggt atgatgggct
     3661 tgattccaat attgttctta atatacccga cagtattagc catagttgca ggaactgttg
     3721 ttatgttatt tatggctaag gttcaaaagc catgggcact atttatattt ggtatgatat
     3781 caccacttgt gatgtttgca gctggtcata cctacgtagt tgtggtttta tcacttatag
     3841 taatgataat agcagaatta attagaaaga ttggtaatta taattcattt aaatacaata
     3901 tgctttctta tgcaatcttc agcacatgga tatgtagctc tttaatgcaa atgcttttag
     3961 caaaagaaaa atatatggag tggtctttga tgacgatggg aaaagattat gttgatgtat
     4021 tagaaaagtt aataatttat cctcacatgg ctttagtagc cttaggtgct ttcttaggag
     4081 gaattcttgg agcatatata ggcaaggctc tattgaaaaa acactttgaa aaagcaggca
     4141 tcgtataatg cctacttttt cttcaaatgc tccctttaat cccaatccga tctctaagat
     4201 attagtcgta tttatcacag gcttaactgt tatgcatagc ataaacatcc gttttgagct
     4261 ggcaattgtt tgtcttattg gtattttgct ttatttgaat ggatacaaaa aaacactttt
     4321 caaatggatt atattgtgtg ggatacttta ctccttgcct aattttatgg tgttatctga
     4381 attaaaccct atagttaaga tgtttttgag tatacctatt gttattagaa tgtttatttt
     4441 accatttatg gcagcaagct ttatgataaa gacctcggat gtaggcgcaa taatttcatc
     4501 gatggataag cttaagattt caaagaatgt atccatacct attgcggtta tgtttagatt
     4561 cttcccatct tttaaggagg agaagaaaaa catcaaaatg gctatgagag taagagggat
     4621 aaattttaaa aacccagtca aatatcttga atatgtttct gtgccactac tcattatatc
     4681 atctaatata tcagatgaca ttgcaaaagc ggcagaaaca aaggcaatag aaaatccaat
     4741 tgccaagacc agatacattc gcgtaaagat acagctaatt gattttgttt atgttttagc
     4801 ggttgctgga cttattgtgg gaggcttaat atggttgaaa taaaaaattt aagtcttgat
     4861 tatggtgaag agcatatatt agatgatata tcactatcca tagccgaggg agagtgcgtg
     4921 ctatttacag gaaaaagtgg aagtggtaag tcatctttaa taaattcaat caatggacta
     4981 gctgtaaggt atgataacgc aaagacaaag ggcgaaataa ttattgatgg taagaatata
     5041 aaaaatttgg aactttatca aatctcaatg cttgtttcaa ctgtttttca aaatcctaag
     5101 acatattttt ttaatgtcaa tacgacatta gaattattat tttatttgga aaatatcggt
     5161 cttgcaagag aagagatgga caggcgtttg aaggatatgc ttgagatatt cccgataaaa
     5221 aatcttttga acagaaatat atttaatcta tccggcggtg aaaaacaaat tctttgcatt
     5281 gcagcttctt atatagcagg tacaaagatt atagttatgg atgagtcttc atcgaattta
     5341 gatattaaaa gcataagtgt tttggcaaag atgctaaaga tattaaaaga gaaaggcata
     5401 agcataattg ttgcagagca tagaatttat tatttgatgg acatagttga ccgtgtattt
     5461 ttaatagata aaggaaagct taaaaaaact tatactagaa gtgaattttt aaa
//
LOCUS       DAVAAL010000118         5497 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVAAL010000118 DAVAAL010000000
VERSION     DAVAAL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.119"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..686
                     /locus_tag="VQM60_001730"
     CDS             <1..686
                     /locus_tag="VQM60_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET1081563.1"
                     /translation="EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTID
                     IGASTGGFTDVMLQNSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQ
                     EPSFASIDVSFISLSLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVH
                     QNVLESVTAMAVEVGFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVE
                     RAHSQFKNE"
     gene            679..1110
                     /locus_tag="VQM60_001731"
     CDS             679..1110
                     /locus_tag="VQM60_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET1081564.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            1117..2784
                     /gene="recN"
                     /locus_tag="VQM60_001732"
     CDS             1117..2784
                     /gene="recN"
                     /locus_tag="VQM60_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET1081565.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            2786..3514
                     /locus_tag="VQM60_001733"
     CDS             2786..3514
                     /locus_tag="VQM60_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET1081566.1"
                     /translation="MTDYYVIGDVHGKAEMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            3593..5416
                     /gene="lepA"
                     /locus_tag="VQM60_001734"
     CDS             3593..5416
                     /gene="lepA"
                     /locus_tag="VQM60_001734"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET1081567.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         1609 a          998 c         1339 g         1551 t
ORIGIN      
        1 gagagaaaat tccagatgac accgaattaa aactcaaggg ggagaaactc aagtatgtca
       61 gccgtggtgg tttgaaactg gaaaaggcct tgcaggtctt tgatttgtcg gtggatggcg
      121 cgactacgat tgatatcggg gcctctactg gaggttttac cgatgtcatg ctacagaata
      181 gtgccaagtt ggtctttgca gtcgatgttg gtaccaatca gttggcttgg aaattacgcc
      241 aagacccacg agttgtcagc atggagcagt tcaatttccg ctatgctgaa aagactgatt
      301 tcgagcagga gccgagcttt gccagtattg atgtgagttt catttccctt agtctgattt
      361 tgccagcctt gcaccgtgtc ttggctgatc aaggtcaggt ggtagcactt gtcaaacctc
      421 agtttgaggc aggacgtgag cagattggga aaaatggaat tattcgagat gctaaggttc
      481 atcagaatgt ccttgaatct gtaacagcta tggcagtaga ggtaggtttt tcagtccttg
      541 gcttggactt ttctcccatc caaggtggac atggaaatat tgaattttta gcgtatttga
      601 aaaaagaaaa gtcagcaagc aatcagattc ttgctgagat taaagaagca gtagagaggg
      661 cgcatagtca atttaaaaat gaataaaaaa gagagacttg aaaaaattag aagattggtg
      721 acagattatc aaatcggcac gcaagaagaa attgtagaac atttgaaaga agcaggtatc
      781 actgccactc aggcgacggt atcccgagat atcaaagagt taggtattgt caaaattcct
      841 ttgagagaca acacctatgt ctacgaattg ccaaaatcaa tcgtaaaaag tctgcaactg
      901 gctgaagaca atatcgaatc ggctgaattg atggataaga tgatcaatct ccaagttatt
      961 ccaggaaata cggcttttgt aaaagctcag ttaatcgaga cttttgcaga caagattttt
     1021 agctgtttga ctgatgatag ctcgatttta gtcattgcca gaagtggaag tctagcagag
     1081 gaaatctttg aacaagtaaa aaattggtag gtctatatgt tacttgaaat ttcgataaaa
     1141 aactttgcca ttattgaggc tatttccctc aattttgaaa aggggatgac tgtcctgact
     1201 ggtgaaacgg gtgcagggaa gtcgattatc attgatgcca tgaatatgat gttgggagct
     1261 cgtgcgacga cagatgttat tcgtcatggt gcaccaaagg cagagattga ggggcttttc
     1321 tcagttgaga atagtcgtct tttacaggaa atttttgatg agcaaggttt agaattaggt
     1381 gatgaaatta tcatccgtcg ggaaatcttg caaaatggtc gtagtatcag ccgtgtaaat
     1441 ggtcagatgg ttaatctgtc tgttttgcga gctattgggc aacatcttgt agatattcat
     1501 ggtcagcatg accatgagga gttaatgcgt ccccaactgc atatccagat gttggatgaa
     1561 tttggtgatg ccgctttttg ggacttgaaa gaaacctatc aaacgagttt tgatgcctat
     1621 cggaaaatgc gcaagcaggt tctggaagtc aagaaaaacc aacaggaaca caaggcacgt
     1681 atcgaaatgt tggaatttca aatggcagag attgaggcag caaacttgca ggctggagaa
     1741 gacttggctc tcaatcaaga gcgagataaa ctcctcaacc acaaaaatat tgcggataca
     1801 ctgaccaatg cctacagtat gttggacaat gaagattttt caagtctggc caatgttcgt
     1861 tcagctatga atgacatgga aagtgtcgaa gagtatgatc ctgaataccg tgaaatttca
     1921 agctctctgt ctgagaccta ctatgtttta gaagacatta gcaaacgttt ggaagctatc
     1981 attgaggacc ttgattttga tggcaatcgc ctgatgcagg ttgagaatcg tttggacctc
     2041 cttcatacta ttactcgtaa gtatggtggg actgttgatg atgttttgct ttattttgcc
     2101 aagattacgg aagaatacaa tctcttgaca ggcaataatc tttcgtctga ggacatggaa
     2161 gcagagctta agaagttgga agtcaatctt gtcaatttgg caggtcaact tgcttctgct
     2221 cgtcataatt tggctcagca actcgaagct gagattaaac aagaactgca agatctttat
     2281 atggaaaaag cccagtttca ggttcgtttt agtaagggaa aattcagtcg tgagggaaat
     2341 aaaatggttg agttttatat ttcaaccaac cctggagaag actttaaacc cttggttaag
     2401 gttgcttctg gaggggaatt atctcgtctc atgttagcca ttaagtctgc cttttcacgt
     2461 aaagaaggca agactagcat tgtctttgat gaggtggata cgggagtttc aggtcgtgtt
     2521 gctcaagcta ttgctcagaa aattcataaa attggtcagc atggtcaggt tttggctatc
     2581 tcccatttgc cacaagtaat tgcgattgca gattatcaat tctttattga gaagattagt
     2641 aatgaccatt caacggtttc gactgttcgt ctcttgacgg tcgaagagcg agtggaggaa
     2701 gttgccaaga tgttggcagg tgatgatgtg acagaagcag ccctgacgca agccagagaa
     2761 ttgttgagaa acagggagaa ataagatgac agattattat gtaattggag atgttcacgg
     2821 aaaagctgag atgctggaag accttctcaa aacctgggat ggtcagcccc agttgctctt
     2881 tctaggtgat ttgattgaca gaggtgagga tagtcaccgt gttcttgaaa tggtcaagga
     2941 cttggtggac aatcaagggg ctatctgttt gtccggaaac cacgagtata tgtttttgac
     3001 ttggctagat gacccagaag aaagttatga ccattatagt cgcaatggtg gagatacaac
     3061 cattaactct atcctaggtc gtcccttgga tgcaccagtt gatggagtag aagatgccaa
     3121 gcgggttgcc gctgaagcgt cagacttggt cgaattcatt cgtcaaatgc catttgtagt
     3181 agagacagac aagtatatat ttgttcacgc aggtattgat ttgactttgg acgactggca
     3241 tgaaacaaca gattataaaa aagtatggct tagaaaacca ttccacggag ctgaaaatca
     3301 tactggaaaa accattgtct tcggtcatac accggtctat ggtttgttac agcaagaacg
     3361 cggtacatct gagctttgga ctacagaaga tggcaagatt ggaatggatg gaggagctgt
     3421 ttatggtggt gtccttcacg ggattgtctt tacagaccaa ggaatgacag aacaccactt
     3481 tatcgaaaat gacggctttg tcgctgaaga ttagtactcc tagcagggta tggtcttgtc
     3541 aaaatatcaa aaacaattta taataaatag ataccctgaa aggaagagaa tcatgaactt
     3601 agaagaattg aaaaaacgac aggggaagat ccgtaacttc tctattatcg cccatattga
     3661 ccacgggaaa tcaactctag cagaccgcat tttggaaaaa acagagacgg tttcaagtcg
     3721 tgaaatgcag gcccagcttt tggatagcat ggatctagag cgggaacgtg gaattactat
     3781 taagttgaat gccatcgagt tgaattacac tgcaaaagat ggggaaactt atattttcca
     3841 cttgattgac acaccagggc acgttgactt tacctatgaa gtttcacgtt cgctagctgc
     3901 ctgtgagggt gctattttgg tggtcgatgc ggctcaagga attgaggctc aaactcttgc
     3961 caacgtttat ctggctttgg ataatgattt ggaaatcatg cctatcatta ataaaattga
     4021 cctgccggct gcagatccgg agcgcgtgcg tacagagatt gaagatgtga ttggtttgga
     4081 tgccagtgaa gcagttttgg cttctgccaa ggctggtatt gggattgaag aaatcctcga
     4141 gcaaattgta gaaaaagtac cagcaccaac gggtgatgtg acggcgccac ttaaggcctt
     4201 gattttcgac tctgtttacg atgcttaccg tggggttatc ctccaagtgc gtgtcatgga
     4261 cggagtggtc aaacctggtg ataagattca gctcatgagc aatagtaaga cctttgatgt
     4321 ggccgaagtc ggtattttta caccaaaagc ggttggtcgt gatttccttg cgactggtga
     4381 tgttggttac attgcggcgt ctatcaagac ggttcaggat actcgtgtgg gtgataccgt
     4441 taccttggca accaatcctg cggcagaacc attacatggt tataagcaga tgaatcctat
     4501 ggtctttgcg ggtctctacc caatcgaatc aaacaagtac aatgacctgc gtgaagcgct
     4561 tgaaaaattg caactgaatg atgctagtct tcagtttgaa ccagaaacat ctcaggcact
     4621 tggatttggt ttccgttgtg gatttcttgg acttctccat atggatgtta ttcaggaacg
     4681 tttagagcgt gagttcaata ttgacctcat catgacagct ccgtctgtta tttacaaagt
     4741 taatttgacc gacggtgagt ctatggatgt gtctaaccca tctgagtttc cagacccaac
     4801 taagattgcg accattgaag agccttatgt caaggcgcaa atcatggtac cacaggagtt
     4861 cgtcggagca gtaatggagc tagctcagcg taagcgtggg gactttgtga ctatggacta
     4921 tattgatgat aaccgtgtca atgttatcta tcaaattcct cttgctgaaa ttgtctttga
     4981 cttctttgat aaacttaagt cttcgacacg tggttatgca agctttgact acgaattatc
     5041 agaatatcgc ccatctaagc tggtgaaaat ggatatcctt ctcaatggag ataaggtgga
     5101 tgccctcagc tttatcgttc acaaggactt tgcctacgaa cgtgggaaac tcatcgttga
     5161 taaactcaag aaaatcatcc ctcgccaaca atttgaagtt ccaatccaag cggctattgg
     5221 acacaagatt gtcgctcgta ctgatatcaa ggcccttcgt aagaacgtac ttgctaaatg
     5281 ttatggtggt gacgtttctc gtaagcgtaa actccttgaa aaacaaaaag ctggtaagaa
     5341 acgcatgaaa tccatcggat cagttgaagt tccgcaagaa gccttcctca gcgtcttgag
     5401 tatggatgaa gaataagtta tcagaaatgc ctttccttgt tcacaggaaa tttatataag
     5461 aatcaatagt aaagcaggct tagatggtct gcttttt
//
LOCUS       DAVAAL010000119         5472 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVAAL010000119 DAVAAL010000000
VERSION     DAVAAL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.120"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..575)
                     /locus_tag="VQM60_001735"
     CDS             complement(<1..575)
                     /locus_tag="VQM60_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET1081568.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTL"
     gene            731..1804
                     /locus_tag="VQM60_001736"
     CDS             731..1804
                     /locus_tag="VQM60_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET1081569.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(1895..2197)
                     /locus_tag="VQM60_001737"
     CDS             complement(1895..2197)
                     /locus_tag="VQM60_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081570.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPNYLSMLFDFY"
     gene            complement(2265..2702)
                     /locus_tag="VQM60_001738"
     CDS             complement(2265..2702)
                     /locus_tag="VQM60_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET1081571.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQISLEKVVADAESQMKD"
     gene            complement(2778..3620)
                     /locus_tag="VQM60_001739"
     CDS             complement(2778..3620)
                     /locus_tag="VQM60_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET1081572.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHIGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            3732..4382
                     /locus_tag="VQM60_001740"
     CDS             3732..4382
                     /locus_tag="VQM60_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET1081573.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            4363..4554
                     /locus_tag="VQM60_001741"
     CDS             4363..4554
                     /locus_tag="VQM60_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081574.1"
                     /translation="MSRKSTNFSSPLPTLKVLFSGFYFYHFILLSFGGRIFEKNGRVF
                     EKKKGSLTYEKITKNFCYI"
     gene            4580..5226
                     /locus_tag="VQM60_001742"
                     /pseudo
     CDS             4580..5226
                     /locus_tag="VQM60_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            5357..>5472
                     /locus_tag="VQM60_001743"
     CDS             5357..>5472
                     /locus_tag="VQM60_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease"
                     /protein_id="HET1081575.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFIPNHSYLSQLDVTYKT"
BASE COUNT         1683 a         1258 c          971 g         1560 t
ORIGIN      
        1 agggtattat catcaatgaa aaagtcactg ttccaaagtt cccgcatcag gtcgtcacgt
       61 gctacgatgt tgcctgcatg ctcaaataac acgcgtaaaa tctggaattc attcttggtc
      121 aaattcaaga cttgcccttg ataatgtaaa tccatggatt tggtattgag gataacacca
      181 gcatattcca gcaaactctc atcacgccca aactcatagg aacgacgcaa caagccctga
      241 accttagcta aaagaacctg ctggtcaaaa ggcttggtca caaagtcatc cgcccccata
      301 ttgattgcca tgacaatatc catagcctgg tctctcgaag aaagaaacat gataggtacc
      361 ttggaaatct tgcggatttc ctgacaccag tgataaccat taaacaaggg caaaccaata
      421 tccatgagga ccagatgagg ttccgactga acaaatagac tcaaaacttc cataaagtct
      481 tctaccagga ccacttcaaa tccccattca gagagcattt tcccaatctg ttgacgaatg
      541 acctgatcat cttctattaa taaaatcttg tgcatgcgct tctccttttc tattattata
      601 acagattttt ccatgctaga tggtctgaaa ctgaatttga aatagcctgt ttttagccag
      661 tacaaacagg ctatgctact agctaatttg agggaaattt gctaagataa ataaaaagaa
      721 aggagctctt atggccaata tttttgacta tctgaaagat gtcgcatatg attcttatta
      781 cgaccttccc ttgaatgagt tagacattct aaccttaata gaaatcacct acctctcctt
      841 tgataatctg gtctccacac ttcctcaacg tcttttagat ctagcacctc aggttccaag
      901 agagcccacc atgcttacta gcaaaaatcg ccttcaatta ttagatgaat tggctcaaca
      961 caagcgcttc aaaaattgca aactctccca ttttatcaac gacatcgacc ctgaactgca
     1021 aaagcaattt gcggctatga cttatcgtgt cagcctcgat acctatctga ttgtctttcg
     1081 tgggacagat gacagtatca ttggctggaa ggaagatttc cacctgacct atatgaagga
     1141 aattcctgct caaaagcacg cccttcgcta tttaaagaac ttttttgctc atcatcctaa
     1201 gcaaaaggtt attctggccg ggcattccaa gggaggaaat ctcgctatct atgctgctag
     1261 ccaaattgag caaagtttgc aaaatcagat cacagcagtt tatacatttg atgcacctgg
     1321 tctccatcaa gaattgacac agactgcggg ttatcaaagg ataatggata gaagcaagat
     1381 attcattcca caaggttcca ttatcggtat gatgctggaa attcctgctc accaaatcat
     1441 cgttcagagt actgccctgg gtggcatcgc ccagcacgat acctttagtt ggcagattga
     1501 ggacaagcac ttcgtccaac tggataagac caacagtgat agccagcaag tagacacaac
     1561 ctttaaagaa tgggtggcca cagtccctga cgaagaactt cagctctact tcgacctctt
     1621 ctttggcact attcttgagg ctggtattag ctctatcaat gacttggctt ccttaaaggc
     1681 gcttgaatac attcatcatc tctttgtcca agctcaatcc ctcactccag aagaaagaga
     1741 aaccttgggt cgccttaccc agttattgat tgatactcgt taccaggcat ggaaaaatag
     1801 ataatactct tgaaaattaa atgtatacaa aacaaaagac ctagaataca tactttcatg
     1861 tgcattctaa gtctttttaa atagaatcta atagtcaata aaaatcaaat agcattgaaa
     1921 gataattggg cttggaacgt ccctctcgct tcaacaaaat gaccccatta tagattaaaa
     1981 agatgccact tagaaaaagc aaaaaaggaa gtaagacaaa ggcaaataca taaaaagcta
     2041 actgaacatt ctcgtatcca tttttataaa aaaggtagga tagataaaaa taacttgaaa
     2101 tgagggataa taaaaataat actggattcc acaaacttct attatccttc caaaatgaca
     2161 ctataaaggc taatacaatt cctataacga gatacatttc ttactccttt aatagctaca
     2221 ttttatcata attatccaaa gaaaaaagag ggcatttatc cctcttaatc cttcatctga
     2281 ctctctgcat cggccacgac tttttctaga ctgatttgac caagttctgc ctccatagtc
     2341 aactgaattc tctccaattt ttgatccaaa acatcatgaa tatgagctcc tacagggcaa
     2401 tttggattcg gattgtcatg gaaactgaag agttgacctg tcttaccaag acattcgacc
     2461 gcctgataaa catctaaaag actaatatcc ttaaggtcct tgacaatctc tgttccgccc
     2521 gttccacgcg ctactgaaat cagctctgcc ttcttcaact gggacaagat ctttctgata
     2581 atgacaggat tgaccccgac actagcagcc agaaaatcac tggtcacctt gctttccttc
     2641 ccctcgaggg caatgattat cagcatatga gtcgcaatgg taaatctact tggaatttgc
     2701 atcctcttct cctttttacg aggctaccct gcctctactc ttctttttct attattatac
     2761 cctttttagt tgtaatgtca atcgttacca cttttcaacc agtcgtctaa ctcccgatcg
     2821 cagccctctt tctgagccaa ttctctcaaa aattcctgat gatgagtatg gtggatccca
     2881 ttgaccagac tttcatagta aacctcaaaa taaggaagtc tcaggtcttt agccagctgc
     2941 aattcagctg ccacatcgta gtctacccgt cggaagtcca tatctaccag gcccttgtca
     3001 tcaaactcca aaatcatata ctgggcccgc aagtccttcc gtagctgagc gtccaaaaag
     3061 aaaggttggc caatcgaacc cggattgaca atcaattgcc caccagtccc gtaacgaagc
     3121 aactgctggt gaatatgtcc ataaacagca atatcacagg gaggatgagt caccaagcgg
     3181 tcaaactcct cttgtttgcc aatatgaatc aactctcgcc cccagttctt atcaggcaga
     3241 tgatggctaa ttcccaccgt caaatcccca aactgacgat gaatttgaag aggttgattg
     3301 tggagcactt caatttcttc tagggaaatt tcctctaaaa catactggca ctggcgcaag
     3361 agatagcgtt gactggggcg agtactatcc aattccttac ggacaccatg ccaaagactg
     3421 tcttcccagt ttcccaaaac tctagccgta atcggtagtt gatccaacaa gtccaaaatc
     3481 cttctacgcc ctgtccctgg catgagaata tctcccaaaa gccagtattc atccactcct
     3541 atctgccgag catctgccaa aacagcctcc aaggcggtgg tatttccatg aatatctgaa
     3601 agaagagcta ttttcgtcat atccatctcc tcgttttttc tcttgcaata agtataacat
     3661 aaaaagtcac agctagagaa atctagcttt ttttgatata ctagataaag atattagaca
     3721 agaggaaacg aatgacccca aacaaagaag actatctaaa atgtatttat gaaattggca
     3781 tagacctgca taagattacc aacaaggaaa ttgcggctcg catgcaagtc tctccccctg
     3841 ccgtaactga aatgatcaaa cgaatgaaaa gtgaaaatct catcctaaag gacaaggaat
     3901 gtggctatct actgactgac ctcggtctca aactggtctc tgagctctat cgtaagcacc
     3961 gcttgattga agtttttcta gttcatcatt tagactatac aagtgaccag attcacgagg
     4021 aagctgaggt cttggaacac actgtctctg acctgttcgt ggaaagacta gataaactgc
     4081 taggtttccc taaaacctgc ccccacggag gaactattcc tgccaaggga gaactcctcg
     4141 ttgaaatcaa taacctccca ctagctgata tcaaggaagc tggcgcctac cgcctgactc
     4201 gggtgcacga tagttttgac attctccatt atctggacaa gcactcactt cacatcggtg
     4261 accagctcca agtcaagcag tttgatggct tcagcaatac cttcactatc ctcagtaacg
     4321 acgaggattt acaagtgaat atggacattg caaaacaact ctatgtcgag aaaatcaact
     4381 aatttctcaa gtcccctacc aaccctgaaa gttttatttt cagggtttta tttctatcat
     4441 tttattttac tttcatttgg tggtagaata tttgaaaaaa atgggagagt atttgaaaaa
     4501 aagaaaggta gtttaaccta tgaaaaaatc actaaaaatt tttgctacat ctaaatgatt
     4561 tgacctcttc ggggttgctt tggtcgttgg gattgcgatt gcatctggtt atctcaactc
     4621 acgcctcgat aaatttgtag actggggact atggacagct cttgtaccct ttggattgat
     4681 ttccgtaacc aacgttggga tttccatgtt gtccactcgt ttcacgggga aattaagcaa
     4741 atggggaaat tactttggta ttgttaatac cattttgtcc ggtgctattg attatatcct
     4801 tagaaataag gcggccatca ttacctatcc cgtcacctta ctcatttata cctttgctat
     4861 aaagaaatgg aaagcttcgc aagaaggcag acccaaccaa atgagccaaa aacaggtaaa
     4921 attggcggcc atcatcattt ccatcatcgc cttcctcttt gcctttgtga ccaactatat
     4981 cggatatggg ggcaagatga atctccttgc ctacgtaaca actattgcct ttgcactgtc
     5041 cctcattgcc aatgctttga atgcattggc aatgagcgac agtggggctt ttggttgatt
     5101 tacaatttcg ttcagctgac aaaagctggt attcaaggaa acttcgccaa tatcggtaaa
     5161 tacatctttt atatcctcaa tgcaatcgga gctttatttg tctggaatga tgaagaagtt
     5221 agataataga aggaaactca aataaagaat actagccaac ttaagagaat ctcccctctg
     5281 ctatttttat cattttagtt gtaactccca ttcttttaga gtagaatgaa gttagataaa
     5341 agagggaggt cttgtcatga aaaaactcta tagaatccat tttatagcaa ttgcagttat
     5401 tgatttgcta ctatttgcat tttttatccc aaatcattca tacctctctc aactagatgt
     5461 aacttacaaa ac
//
LOCUS       DAVAAL010000120         5261 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVAAL010000120 DAVAAL010000000
VERSION     DAVAAL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.122"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            41..244
                     /locus_tag="VQM60_001744"
     CDS             41..244
                     /locus_tag="VQM60_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081576.1"
                     /translation="MNLIAEKVSKTLDKTFDKDVREIPTSQFYQKFVDEMGRTYSGNL
                     ILQELITVNGAYKATYIGELSSN"
     gene            375..1547
                     /locus_tag="VQM60_001745"
     CDS             375..1547
                     /locus_tag="VQM60_001745"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET1081577.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTVLSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1547..2821
                     /locus_tag="VQM60_001746"
     CDS             1547..2821
                     /locus_tag="VQM60_001746"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET1081578.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(2856..3821)
                     /locus_tag="VQM60_001747"
     CDS             complement(2856..3821)
                     /locus_tag="VQM60_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET1081579.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILIIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3802..5256)
                     /locus_tag="VQM60_001748"
     CDS             complement(3802..5256)
                     /locus_tag="VQM60_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET1081580.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFIEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
BASE COUNT         1477 a         1070 c         1218 g         1496 t
ORIGIN      
        1 aacaacctat cgaaatcatc gtaaacgctt cggattacga atgaatttga ttgctgagaa
       61 ggttagtaaa acacttgaca agacatttga taaggatgtc agagaaattc cgaccagtca
      121 gttttatcaa aaatttgtag atgagatggg aaggacttac tcaggaaatt tgatcctcca
      181 ggagctgata actgtgaatg gagcttataa agctacttat atcggtgaac tctctagcaa
      241 ctaatcattc tttaaaatga tgaccaataa gaggcttgat ttggtgaatc aagtcttttt
      301 tctctctttt actgccattt gtgataaaat agtatagaac gattttttac atgaatgata
      361 aaacagaggt aaatatgaca atcggtattg ataagattgg ttttgcgacc agtcaatatg
      421 tcttgaaatt acaagactta gcagaagcga ggggaattga ccctgaaaaa ttaagtaaag
      481 gactcttact caaggaattg agtattgcgc ccctaactga ggacatcgtg accttggcgg
      541 ccagtgctag tgactctatt ttaactgagc aagaaagaca agaagttgac atggtcattg
      601 tggcgaccga gtcaggaatt gaccagagta aggctgcggc cgtctttgtg catggcttgc
      661 tgggcatcca gccctttgct cgtagtttcg agattaaaga agcctgctat ggggcgactg
      721 ctgccctcca ttatgccaaa ttgcatgtgg aaaattctcc ggagtccaag gtcttggtca
      781 ttgcaagtga tattgccaaa tacggtattg aaactccggg agaacccact cagggtgccg
      841 gaagtgtggc tatgttgatt acacaaaatc cacgcatgat ggcctttaat aatgacaatg
      901 tagctcagac ccgtgacatc atggatttct ggcgaccaaa ttactcgaca actccttatg
      961 taaatggtgt ctattctacc caacaatact tggatagttt gaaaacgact tggcttgaat
     1021 atcaaaaacg ctaccagctt actttggatg attttgcggc tgtttgtttc cacttgcctt
     1081 atcctaaatt agcgctaaaa ggcttgaaaa aaatcatgga taagagcctg cctcaagaga
     1141 aaaaagacct cttacaaaag cattttgacc agtctattct ctacagtcaa aaggtgggga
     1201 atatctacac aggttcactt ttccttggac ttttgtctct cttggaaaat acagatagct
     1261 tgaaagctgg ggataaaatc gccctttata gttacggaag tggagctgtg gctgagttct
     1321 tcagtggtga attggttgaa ggatatgaag cttatttgga taaagaccgc ttgaacaagc
     1381 tcaaccaacg aactgtctta tccgttgcag actatgaaaa ggtctttttt gaggaagtaa
     1441 acttggatga aacaaactct gcccagtttg ctggctatga aaatcaagat tttgccttgg
     1501 ttgaaattct cgaccaccaa cgccgttata gcaaggttga aaaataatga agataagttg
     1561 gaatggattt tctaaaaaat cataccaaga gcgcctcgag ctgttaaaag ctcaggcgct
     1621 ccttagtcct gagagacaag ctagtctgga gaaggatgaa cagatgagcg tgactgtggc
     1681 agaccagctg agtgagaatg tagtgggaac tttttctctg ccttattcac tggttccgga
     1741 ggtacttgtc aacggtcagg aatacaccgt tccctatgtg acagaagaac cctctgtggt
     1801 tgcggcggcc agctatgcca gcaaaatcat caagcgtgca ggtggtttta ctgcacaagt
     1861 ccatcagcga cagatgattg ggcaggtagc cctttatcaa gttgctaatc ctaaactagc
     1921 gcaagagaag attgccagca agaaagcgga gctcttggag cttgccaatc aagcctatcc
     1981 ttctatcgtt aaacgtggag gtggggcgcg tgatctgcat gtcgagcaga taaaaggcga
     2041 accagacttt ctcgttgttt atattcatgt cgatacccag gaagccatgg gtgccaatat
     2101 gctcaacacc atgctggaag ccttgaaacc agtcttagaa gaactcagtc agggacagag
     2161 tctcatggga atcctgtcca actacgcgac cgattctctg gtgactgcaa gctgtcgcat
     2221 cgcctttcgc tacttgagcc gccaaaagga tcaaggacga gagattgcgg agaaaattgc
     2281 gttggctagt cagtttgcgc aggctgatcc ttaccgagct gctactcata ataaaggaat
     2341 ttttaatggt attgatgcga ttttgattgc cactggtaat gactggcgtg ccatcgaagc
     2401 tggggcccat gcctttgcca gtcgagatgg acgctatcaa ggtcttagtt gctggacgct
     2461 ggaccttgaa agagaagaat tggtcggtga gatgaccctg cccatgcctg tagcgactaa
     2521 gggtggctct atcggcctca acccacgtgt agctctcagt catgatctac taggaaatcc
     2581 ttctgccaga gaattagccc agattatcgt gtccatcggt cttgcccaaa attttgcagc
     2641 cctcaaagcc ttggtaagta cgggcatcca gcaaggccac atgaaactac aggccaaatc
     2701 cctagctctc ctagctgggg ctagtgaatc tgaagttgct cccctagtag agcgcctcat
     2761 ctcagataaa acctttaacc tagagacagc ccagcgctat ctcgaaaatt taagatcata
     2821 aaaactcaga cgaatcggtc tgagttttct tgtgtttaaa tactttttcc tggtaatagt
     2881 gtaactggta agaagtaacc agttgtggcg acttccttgc cttcaatctt ttgcaagaga
     2941 agatcaatag tgagacaagc aatctcttcc aaaggttgct tgatagtagc caattgaggg
     3001 tagtaatttt cgataaagta ggtcccatca tagccgataa ccttgagctc ttttgggaca
     3061 gaaatgccca attcttgagc gattttaatg atcagaatag ctgtcaaatc atccgaagca
     3121 aaaatggcat ctggtttttc ccgggtcaag atattcttga tttccatttc ttttctgacg
     3181 ggagaaaagt cactggaaac attgataata ggagcttttg ggagtacgga tgcaaaacca
     3241 gcgtggcgca gtccggttgg cgaattggaa ttgtcattcc ctgtaatcat gatgatagac
     3301 tgggcacctg tcttgaccaa ggtttgggca gcaagaaccc caccagcata gttgtcagag
     3361 gagacaacag ggatgtctgg cgataggttt cggtcaaagg aaataatcgg cgctgtcaca
     3421 cgattgtagt cttcgattcc taggttgtga ctaccagaaa tgatgccgtc tacctgattg
     3481 gcttccaaca tttcgatgta ttcgcgttcc ttctcagaat catgttcact gttgcagatg
     3541 atggtcttgt aaccattttt gaagagttgg tgttccaatt tatcaatcaa ttctgcatag
     3601 aaaacattgg aaatattggg gaaaatcaag ccgattaact tagctgattt tccttgcaga
     3661 ctacgagcca ggttgttggg tttatagcct aattctctca tggcttcatt gactttttgg
     3721 atggttttct cagatagata tcctttttta ttgataaccc gagaaacggt agtaggactg
     3781 acgcctgcaa gtttggcgac atcagttagt tttgcgacca taatctaatt catagtaagt
     3841 tccagttggg tttccagatt tgatcaggat accattttgg tccgcatgtg ggaagacacg
     3901 accagaaaat actttttctc ctttattgat gaaaatttca aagacagagt tatcgatgaa
     3961 gattgtagca gtagtagcct gattctcgat agggcaagaa cgagttgtcc caaattcttg
     4021 ggcatattgt tctccagcct ggctacgatc cactgtcact tgaccgttta caaggtcaaa
     4081 gttgattgaa agtcccttac cttctttatc agcaagtaag acaatctcgc tctggctatt
     4141 agcttccaag ttgagttcaa gttcgtaagt gttcttggtt tgggaacggt ttgagaaggc
     4201 ttcttcagaa gcacgaaggt ccttaatagc agcgactgga tactggtaga gcttgtcgtc
     4261 tttgatagtg agttccttga ccaaagagaa ggttccttgg tggtcaaaac ggtcagatgg
     4321 gtaagaaaca tctggcaaac caagccagct aactgctaga gcacgcccat caggagcgtt
     4381 gaaggcttga gttgcatagg cttcgaaacc gtaatccatg ttttgaagtt gagacacatc
     4441 taccattttg gcatttttag ggtcaaagga agccccgatc ttatacatat ttggaaagat
     4501 attatcgtag tctagaactt tcttatccaa tccttgtgga cagtagagaa ggacaggttg
     4561 ttcctctata aagaccagat taggacattc catcatgtag gcagtacggt cgttagcaaa
     4621 gtcaaggtcg ccaactgctt gccagtttgt gtagtcgtta ttgacagcct tgtagagacg
     4681 gacgaagcct tttttctcca agtcctgtcc accgacgaca gcatagtatt gacctttaaa
     4741 gttaaaaatt tgtggatcgc ggaagtggtc agtagagtct gctggctggt caatcaagat
     4801 cttgtcaatc tttgtaatct taccctcctt gtccatcaaa gcaccgattt ggtatgggtg
     4861 acggatccag ttttcatcgc gaacatttcc tgtataaaat aggaataagt tatcgccaaa
     4921 ttgcatggca gaaccagagt aggcaccgtg gctatctaat ggagtatctg gtaaaacttt
     4981 gattccagtt tctttaaagt gaatcaaatc atcactttct agctgtgccc aagattttaa
     5041 accgtgggct gcaccaaaag gaaaattctg gtaaaagagg atccacttgc catcaaagta
     5101 agaaaagcca tttgggtcgt tgagaagtcc tgtttttggc tcaacatggt aatgagtatg
     5161 ccatggagat tgtgccatat tttcctttat atgttgaatt tcttcttgcg tccaatcttg
     5221 ataaagtctg taacgacgct cagttgtcca ttccattcat t
//
LOCUS       DAVAAL010000121         5219 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVAAL010000121 DAVAAL010000000
VERSION     DAVAAL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.123"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(111..1160)
                     /locus_tag="VQM60_001749"
     CDS             complement(111..1160)
                     /locus_tag="VQM60_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1081581.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWIFVQTLFLLLFAPLFLVMGYGLPVFLVYVLLLGVGKYFLFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKVVQKVSGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSLLAQVFIEQSWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLISFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(1157..1888)
                     /locus_tag="VQM60_001750"
     CDS             complement(1157..1888)
                     /locus_tag="VQM60_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081582.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            1956..2366
                     /locus_tag="VQM60_001751"
     CDS             1956..2366
                     /locus_tag="VQM60_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET1081583.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            2376..2663
                     /locus_tag="VQM60_001752"
     CDS             2376..2663
                     /locus_tag="VQM60_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081584.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(3341..4477)
                     /gene="dnaJ"
                     /locus_tag="VQM60_001753"
     CDS             complement(3341..4477)
                     /gene="dnaJ"
                     /locus_tag="VQM60_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET1081585.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEVKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFATAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(4682..5035)
                     /locus_tag="VQM60_001754"
     CDS             complement(4682..5035)
                     /locus_tag="VQM60_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081586.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDYTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(5048..>5219)
                     /locus_tag="VQM60_001755"
     CDS             complement(5048..>5219)
                     /locus_tag="VQM60_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET1081587.1"
                     /translation="DDMKAKLEALNEKAQGLAVKLYEQAAAAQQAQEGAEGAQATGNA
                     GDDVVDGEFTEK"
BASE COUNT         1623 a         1190 c          990 g         1416 t
ORIGIN      
        1 agattttcga agagtataag tctctacgga ccaaccatta tcctgtagta acaaaaaaga
       61 agctgagaca aactgaactc aacttctttt ttagtgtcgt atcagcaatg ttagtcctgc
      121 atctgacgtt ttacctgata aggcaaatac aagactagta aaaccaaacc agctcctagt
      181 aaggctagaa gggctagttt ttcttggaag gtaatcaacc caacaactaa ttccacaagt
      241 aaaacaaaac tgatcaatcc tcgaactacc tcctgtaagc ctttttcctt ttgccccttg
      301 tccagcggaa agagttgggt caaatactgg tagtcaaagg catgatagag ggccagcaac
      361 tggaaaagca agaggtagtt aaagagaact accactgctg tcgcaatcca agattgctcg
      421 ataaagacct gcgccagcaa agaaagcaag agcagacgga gactaagggc aaaaaggtct
      481 ccatttcgca aataagaacg cagatagaga ttttgccaaa tcttcccaga caccttctga
      541 acaaccttta gaataaagtc cagataggca cgacgcttga cgctgtttga aattcccttg
      601 acctgcgtaa agagggcaaa gaaacgaagc aagacttgct tacgcttgct ttcttgggaa
      661 atgacatagt cccaatccag tccagtttca gtgaaaaatt tgctggcctt ttgacgaaag
      721 aggaaatatt ttcctacccc caataaaagc acatagacca gaaaaacagg caagccataa
      781 cccatgacta aaaacaaggg cgcaaataac agcaagaaga gggtctgaac aaaaatccaa
      841 aagactaggg aaatgccggt ttgacgcttg agatggagct tgatttcctc ttctccaact
      901 aagagaaaga gcttgtctgg agcctccata taggtggcaa ttcctcccca aagtaaaagt
      961 aaaacagacg taattcctac aaacaaaagg ataggccaat gattttcagg aaaatgttgt
     1021 aagagttgac tgtactggta ggctagaaag cccaacagga caagcaggaa caagacaaag
     1081 tggtcattga gcacatagcg cagataacaa agacactcct tacgaaaggc ctgctttctc
     1141 tttaaaaaca agtctttcat agatcctcct ctttggtcag agccaagtaa atatcattca
     1201 aactagcctc aggcatatca aaggcttcac gtagttgcag gagattgcct ttggcacgca
     1261 cctctccctt gtgaagaatg acaaaggcat cacacatctt ttccgccgaa tccagcacgt
     1321 gggtactcat gagaatagac ttgccctttt gcttctccac ttccaaaagc tgaatcaaat
     1381 cagaaatagc cagcggatca agaccaagga aaggctcatc cacgatgaaa agacttggat
     1441 ccaccacaaa agcacagata atcatgacct tctgcttcat cccttttgaa aaatgaacag
     1501 ggaaccagtc taatttctgt tccaaacgga acatttttaa caagggctct actcgttcga
     1561 aagccacttt ttgctcaata ccgtaagcca tagcaaccgt ttcgatatgc tctctgaggg
     1621 tcaattcctc atacagacta ggcgtctcag gaatgtagcc aatctgcttg cggtagctag
     1681 tcgcatctcc ttgcagagtc aggccattga tattgatgga gccactataa ggtgccaaca
     1741 gaccgataat ctcattgatc gtcgttgatt tcccagcacc attgagacca atcaaaccga
     1801 ccaactgccc actttcaaca gtaaaggaca catctttcaa aacaggaaca tgaacatagc
     1861 cacctgtcag gtttttaatt tctaacatat tttctccaaa tctggtataa tgtagctata
     1921 ttatatcaaa attcagtaca gtagaggtag attttatgtc agattgcatt ttttgtaaaa
     1981 tcatcgcagg ggaaattcct gcttcaaaag tatatgaaga tgagcaggtc cttgcctttc
     2041 ttgatatctc tcaagtaaca ctaggacaca ccttggtcgt gccaaaagaa cactatcgca
     2101 atcttttgga gatggatgct acgagcgcca gccaactctt tgcccaagta ccaaaagtag
     2161 ctcaaaaagt catgaaagtc actaaggctg ctggtatgaa tatcatttcc aactgtgaag
     2221 aagtcgctgg tcaaacagtt tttcatactc acgttcacct tgtgcctcgc tacagtgctg
     2281 acgatgacct caagattgat tttatcgccc acgaaccaga ctttgacaaa cttgctcaag
     2341 tcgctgaaac catcaaaaac gcttaaggag ttgctatgaa attatccaac ctactgctat
     2401 ttgcaggagc tgcagccgga agttatctgg ttacaaaaaa tcgccaaacc atcacagatg
     2461 aagtcttgaa taccactgac cgcgttcaag ctatcaagga cgatgtggat attatccaaa
     2521 acagcctgca aatcattaac cagcaaaaag aacttatcaa ggaataccaa gaagacttga
     2581 cttacaagtt taaggtcttg gaaaaggata tccagactag actagctgtg ataaaagaaa
     2641 tgcagggaac tgaagataag taaaaagagc ccgacggctc ttttttaaaa cgtaaacaag
     2701 ttatagtgaa ttgaatttag aatagcacat catagtttct aaagcattgt tagagattac
     2761 tttaaaatcc tcttatcaat ttgttcatat tctatttcaa tctactatat atatcccatc
     2821 aactatgcat cataatttag gtaactcata ctcaataaaa atcaaaaagc aaactagaaa
     2881 gctaggcaca gactgctcaa aacaccgttt taaggttgtg gatagaactg acgaagtcag
     2941 taaccatacc catgacaagc cgacgctgac gtggtttgaa gaaatttttg aagagtatca
     3001 ttcaccattt cacaactaaa tcgaagactt tctgccgtaa tgaaaacacc tgaaacagct
     3061 tggtttcagc tgtccggaaa ctttaagact taggttcaaa gtttaggtat ggaacttcga
     3121 aggaggtcgc taccgtccgt cattacttag gaaagtctta aaaaatctat aaagtaaaaa
     3181 gagcccgaca gctctttttg tttatactca atgaaaatca aaaagcaaac taggaaacta
     3241 gccacgggct gctcaaaaca ctgttttgag gttgtagata cgactcacat atatacggca
     3301 aggcaacgct gacgtggttt gaagagattt tcgaagagta ttattctcca tcaaaggcat
     3361 cttttatatg gtcaaagaag ccttttttct ttggatttac tttcaagtca ccagcagtcg
     3421 cgaattcttt caaggctact ttttggcggt cgttcaagcc tgtcggtgtt acgacattaa
     3481 cagtaacgta ttggtcacca actgcaccgc cacgaaggct cggtgccccc ttactacgta
     3541 ggcggaattt cttaccagtc tgagttccct ctggaataac caattcaaca tcaccgtgaa
     3601 cagttggaat atctactgta tcaccaagag ccgcttggac aaagttgagg ttgagattgt
     3661 agaagatagt cgttccttca cgttcaaact tgtcgctagc ttctacagaa actactacat
     3721 acaagtcacc ataaggtcca ccgttaaagc ctgcttcacc ttgaccagcg aggcgaattt
     3781 gttgacctgt ttccacacca gcagggattt tcacatgtac gctatgagct tgtttctcat
     3841 gacctgttcc atgacaggtt gtacatggat atttgacttc ttttcctcga ccgtgacaga
     3901 catcacaggt tacttggcga cgcatcatac caagaggagt ctgcgtatcg acgttaatga
     3961 caccagcgcc atgacagcgt ccacaagtga ctggacttgt ccctggctta gcaccagagc
     4021 cattacatgt acgacagcca gcttcacgat gatacttaac ttccttctca gttccgaaga
     4081 tagcttcttc aaatgtcaaa ttgacacgat actggagatc atctccttgg cgaggagcgt
     4141 ttggattgcg cgaagaaccg cctccgccga agaaacttga gaaaatatcc tcaaaaccac
     4201 cgaagccacc tgccccattg aaaccaccga aaccaccagc tccaccaaaa ccaccattgg
     4261 cgcctgcagc accatactgg tcataggcag cacgtttttg gtcgtcactc aaagtctcat
     4321 aggcttcttg aacttccttg tacttgtcct cagcaccagg ctccttgttg atatctgggt
     4381 gatatttttt ggaaagctta cgataagcct ttttgatctc gtctgccgaa gcgttttttg
     4441 acacccccag acgatcataa aattcagtat tgttcataca agataccaag accgtaaaat
     4501 tcgactgaaa aataggaaat ctgacgctgg agcgacgctc ctagacagat ttctcttttt
     4561 tccgagaatt taggtcgggt tcaattcctt tcttttatat tgatgagata atactccagc
     4621 cactcggccc tatccctttt taccgaaaaa cagaggctgg gttgcaacct ctgtggttag
     4681 attattcatt tgaaataaaa atggttgtat tatctctagt agtaatcttc aaagaccttt
     4741 tagctaaatc aaactcaaat ttattatagt gtgggtcttg tgttttatat aggtgtaact
     4801 cctgacatat atgatccgaa aagtaaatca taacatcttt aaacacagaa gtgtagtcat
     4861 atttactctt accaacagct acttctgtaa attctttata aatgacattt ccatttcttt
     4921 ccacagtcac cagcatttct gttggattat aatccaaatt aaataattct tcaaaaaact
     4981 ttatcttttc atgataatct gcaatgtcag aaagattatt tttattaaca ctcataacac
     5041 tagcccctta tttttcagta aactctccgt ctacgacgtc atcgcctgcg tttcctgttg
     5101 cttgtgcgcc ttctgctcct tcttgagctt gttgcgctgc tgcggcttgt tcgtagagtt
     5161 tcacagcaag gccttgagct ttttcgttca acgcttcaag tttcgctttc atgtcgtcc
//
LOCUS       DAVAAL010000122         5199 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVAAL010000122 DAVAAL010000000
VERSION     DAVAAL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.124"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            307..2808
                     /locus_tag="VQM60_001756"
     CDS             307..2808
                     /locus_tag="VQM60_001756"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET1081588.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAYLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVTTDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYIDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            2947..3789
                     /locus_tag="VQM60_001757"
     CDS             2947..3789
                     /locus_tag="VQM60_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081589.1"
                     /translation="MNRLVNDSRKCEILLFKISIIVVSVIVLVMSIVGAYYFKNCLWD
                     KESLNLTLTLDTLFAVFITFFSFSNDKSSNIFGISPNEIIRTQSEVYQFLFTKSFATL
                     LIVTNLLSIIHYFGAKNLIVSFNETYPRIQSSVGIMWLPLFGILIIYFIWLLIEGLKY
                     IRKINYIIQNREIIYFYSQVKVVDKLTDSESKKYGEYIDLYESCRQNNLSVLLNFLQN
                     SDDKRGVIASNSVRIMFESLDKADQIAALNICRNYVARENVLNKEDAEKLLNEIDKKL
                     EISR"
     gene            3845..4540
                     /locus_tag="VQM60_001758"
     CDS             3845..4540
                     /locus_tag="VQM60_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081590.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            4552..4968
                     /locus_tag="VQM60_001759"
     CDS             4552..4968
                     /locus_tag="VQM60_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081591.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
BASE COUNT         1609 a          924 c         1142 g         1524 t
ORIGIN      
        1 agattcagtc tactatacgt tcttcataaa tcaaaaagcg acataggtag tcggctattt
       61 attgtgaata cattaattag cattccagtt ttatcttcgg tctaaaataa gtattttgtg
      121 ctatacgaga taagcttctt gacttactcc ttgatttact gcataacaat gggataaaaa
      181 gtgggagata gagcaattca tagtcatcaa aattaatgag atacagtata cagttttttc
      241 tttaaacaca tttcaaattc cctcaaaaat ggtataatag taacatcaca aaattggaga
      301 gagaccatga gtttttataa tcataaagaa attgagccta agtggcaggg ctactgggca
      361 gaacatcata catttaagac aggaacagat gcatcaaaac ctaagtttta tgcgcttgat
      421 atgttccctt atccgtctgg agctggtctg cacgtaggac acccagaagg ttatactgca
      481 accgatatcc tcagtcgtta caaacgtgcg caaggctaca atgtccttca cccaatgggt
      541 tgggatgctt ttggtttgcc tgcagagcaa tacgctatgg atactggtaa tgacccagca
      601 gaatttacag cggaaaacat tgccaacttc aaacgtcaga tcaatgcgct tggattttct
      661 tatgactggg atcgtgaagt caacacaaca gatccaaact actacaagtg gactcaatgg
      721 attttcacca agctttacga aaaaggcttg gcctatgaag ctgaagtgcc agtaaactgg
      781 gttgaggaat tgggaactgc cattgccaat gaagaagtgc ttcctgacgg aacttctgag
      841 cgtggaggct atccagttgt ccgcaaacca atgcgccaat ggatgctcaa aatcacggct
      901 tacgcagagc gcttgctcaa tgacttagat gaactagatt ggtcagagtc tatcaaggat
      961 atgcaacgca actggattgg taaatcaact ggtgccaatg taactttcaa agtaaaagga
     1021 acagacaagg aatttacagt ctttactact cgtccggaca cacttttcgg tgcgactttc
     1081 actgtcttgg ctcctgaaca tgaattagta gacgctatca caagttcaga gcaagcagaa
     1141 gctgtagcag actataaaca ccaagccagc cttaagtctg acttggttcg tacagacctt
     1201 gctaaggaaa aaactggtgt ttggactggt gcttatgcca tcaaccctgt caatggtaag
     1261 gaaatgccaa tctggattgc ggactatgtt cttgctagtt atggaacagg tgcggttatg
     1321 gctgtgcctg cccacgacca acgtgactgg gaatttgcca aacaatttga ccttccaatc
     1381 gtcgaagtac ttgaaggtgg aaatgtcgaa gaagctgcct acacagagga tggcctgcat
     1441 gtcaattcag acttcctaga tggattgaac aaagaagacg ctattgccaa gattgtggct
     1501 tacttggaag aaaaaggctg tggtcaggag aaggttacct accgtctccg cgactggctc
     1561 tttagccgtc aacgttactg gggtgaacca attccaatta ttcattggga agatggaact
     1621 tcaacagctg ttcctgaaac tgaattgccg cttgtcttgc ctgtcaccaa ggacatccgt
     1681 ccttcaggta ccggtgaaag tccactagct aacttgacag actggcttga agtgactcgt
     1741 gaagatggtg tcaaaggtcg tcgtgaaact aacaccatgc cacaatgggc tggttcaagc
     1801 tggtactacc ttcgctatat tgacccgcac aatactgaga aattggctga tgaggacctc
     1861 ctcaaacaat ggttgccagt agatatctac gtgggtggtg cggaacatgc tgtacttcac
     1921 ttgctttatg ctcgtttctg gcataaattc ctctatgacc tcggtgttgt tccgactaag
     1981 gaaccattcc aaaaactctt taaccaaggg atgattttgg gaacaagcta ccgtgaccac
     2041 cgtggtgctc ttgtgacaac cgacaaggtt gaaaaacgtg atggttcctt cttccatgta
     2101 gaaacagggg aagagttgga gcaagcgcca gccaagatgt ctaaatcgct caagaacgtt
     2161 gttaacccag acgatgtggt ggaacaatac ggtgccgata cccttcgtgt ttatgaaatg
     2221 tttatgggac cactcgatgc ttcgattgct tggtcagaag aaggtttgga aggaagccgt
     2281 aagttccttg accgagttta ccgtttgatt acaagtaaag aaatccttgc ggaaaacaat
     2341 ggtgctcttg acaaggctta caacgaaaca gtcaaagctg ttactgagca aattgagtct
     2401 ctcaaattca acacagctat tgcccaactt atggtctttg tcaatgctgc taacaaggaa
     2461 gataagcttt atattgacta tgccaaaggc tttattcaat tgattgcacc atttgcacct
     2521 cacttggcag aagaactctg gcaaacagtc gcagaaacag gtgagtcaat ctcttatgta
     2581 gcttggccaa cttgggacga aagcaaattg gttgaagatg aaattgaaat tgtcgtccaa
     2641 atcaaaggaa aagttcgtgc caaactcatg gttgctaaag atctatcacg tgaagaatta
     2701 caagaaatcg ctttagctga tgaaaaagtc aaagcagaaa ttgacggtaa ggaaatcgtg
     2761 aaagtaattg cggtaccgaa taaactcgtt aatatcgtcg ttaaataacg agtttattag
     2821 ctctatctgc caccttcaat agtccactgg actattgaag ccaactaaat tagttaacat
     2881 tgttgtgaaa taaaaagaag taactctaga gtatgaactt tagagttact tttagaatag
     2941 gtcagaatga atagattagt gaacgatagc aggaagtgtg aaattttatt atttaagatt
     3001 tctattattg tagtgtcagt tatagtatta gtaatgtcaa tagtgggtgc atattatttt
     3061 aaaaattgtc tttgggacaa ggaaagttta aatttgacac tcactttaga cactctcttt
     3121 gcagttttta ttactttctt tagtttttca aatgataaat caagcaatat ttttggaata
     3181 tctccaaatg aaattataag gacacagtct gaagtgtatc aatttttatt tacaaaatct
     3241 tttgcaactc tattaatagt gacaaatttg ttatccatta ttcattattt tggagccaaa
     3301 aatttaatag tttcttttaa tgaaacttat cctcgtattc aatccagtgt tggtatcatg
     3361 tggctaccac tgtttggtat tctgataatc tattttattt ggctcttaat agagggatta
     3421 aaatatatca gaaaaataaa ttatattatt caaaataggg aaataatcta tttttattcg
     3481 caggtaaaag ttgtagataa acttactgat tctgaatcga aaaaatacgg tgaatatatt
     3541 gatttgtacg aatcgtgtcg tcagaacaat ttaagtgttc tacttaactt tcttcaaaat
     3601 tcggatgata aacgaggagt tattgctagt aactcagtga gaattatgtt tgaatctttg
     3661 gataaagcag accaaattgc ggccctgaat atttgtagaa actatgtagc acgagaaaat
     3721 gttttaaata aagaagatgc agaaaaattg ttaaatgaaa ttgataaaaa acttgaaatt
     3781 agcagatagg acaattcaaa tagagttgag taaaaagttt gaaaagtgaa acaaggagaa
     3841 agctatgcca gtaaatgaat atggtcagat gattggtgag tcaatggaag gttatacacc
     3901 cggtgcactg ccttctattg atttcttaga agggcgttat gctaggatag aggctctctc
     3961 ggtagaaaaa catgcggagg atttgctagc tgtttatggc ccggatactc ctcgggagat
     4021 gtggacctat ctttttcagg agccagtagc agatatgggg gagctggtta gccttttaaa
     4081 tcagatgttg gctcgtaagg accgttttta ctatgcaatc atagacaagg cgactggtaa
     4141 ggctttggga actttttctc tcatgcgcat tgaccagaat aaccgagtaa tagaagtcgg
     4201 agcagtcact ttttctccag agctaagggg tacacggata ggaactgagg ctcagtatct
     4261 cctagctcgc tatatttttg aggagctaaa ctatcgtcgc tacgagtgga aatgcgatgc
     4321 cttaaatctg ccatccagac gagttgcgga gcgtttggga tttgtctatg aaggaacctt
     4381 ccgccaggcg gtggtttata aggggcgtac gagagatacg gattggttgt ctatgattga
     4441 tatggactgg cctcaagtca aagatcgttt ggaaacatgg ctgcgtcctg aaaattttga
     4501 taaaaatgga cagcagtaca agagcttgag agaactttaa gaggtgttga gatgattact
     4561 attaaaaagc aagaaattgt caagctagag gatgttttgc atctctatca ggctgtcggt
     4621 tggacaaatt atacccatca accagagatg ctggagcatg ccttatcgca ttcattagta
     4681 atttatctgg cacttgatgg tgatgctgtg gtgggcttga ttcgtttggt tggagatgga
     4741 ttctcatcag tttttgtaca ggatttgatt gttttaccaa tctatcagcg tcaagggatt
     4801 ggtagtgccc taatgaaaga ggccttaaaa gattacaaag atgcttatca agtccagctg
     4861 gtgacagaag agacagaaaa aaacgtggga ttttatcgtt ctatgggctt tgaaatctta
     4921 tccacctata attgtatagg gatgacttgg gcgaatcgag aaaaataaca aaacttgttt
     4981 tttcttaagc aaaatttaag gatggtctag tattatatag tcattaaata aagacctcct
     5041 aactttattt aataaaatcc taaacttttt tcatcacaat ctcctaatga agccacccaa
     5101 tcaggtggct tttttgcgtg gtgaggtgct ggtgatagaa attttttgca aaatagtaaa
     5161 attttagaaa agttaagcta gttttaagtt tcgtcttgt
//
LOCUS       DAVAAL010000123         5063 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVAAL010000123 DAVAAL010000000
VERSION     DAVAAL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.125"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(560..724)
                     /locus_tag="VQM60_001760"
     CDS             complement(560..724)
                     /locus_tag="VQM60_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081592.1"
                     /translation="MDESKYLCYLEKYDALGSGRLLAKVNNTYYMFNKNQIEFLRYSN
                     KNAKKERKYM"
     gene            complement(1111..1971)
                     /locus_tag="VQM60_001761"
     CDS             complement(1111..1971)
                     /locus_tag="VQM60_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET1081593.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(2246..3232)
                     /locus_tag="VQM60_001762"
     CDS             complement(2246..3232)
                     /locus_tag="VQM60_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET1081594.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(3504..4979)
                     /locus_tag="VQM60_001763"
     CDS             complement(3504..4979)
                     /locus_tag="VQM60_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET1081595.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
BASE COUNT         1498 a          993 c          895 g         1677 t
ORIGIN      
        1 taaaatatca tgaaaaatat agcagataaa taaaaaatac ttatcataga aaacaggaac
       61 atcgttgttc taaaaacttt cttttcacta taactagcac cccattctgt tccaaaagat
      121 atgactacga gataaacaat tctaatatat atgcgtccag gtactctccc tgacttcaaa
      181 taatatatca ttattaatag ctcgtgtata atagctacca cgcttcctac taaaaatgtg
      241 ataatatttc ttggaacttt tgaaatgttt agttgaatat cataacaagc ataacaaaga
      301 aataaagcta aaataggact acaatatagc attacttttg taattagtac aggattcgga
      361 aggaaaggta taaatttaga ttttttttgt ccaatataat catttaattt ttgtggaata
      421 tagaatcttg tcaatctgcg atatattgac aaaaacttca acccattatc atcaaatgaa
      481 tttttcttaa caagtattcc ttgtttgtaa aaataatcaa taaattggtc tatggtatta
      541 ctagtatagc aggaaaactt tacatatatt tcctctcttt tttagcattt ttatttgagt
      601 atcgtaagaa ttcaatctga tttttgttaa acatatagta agtattatta actttagcta
      661 ataatcgtcc tgagcctaaa gcatcatatt tttcaagata gcacaaatat tttgattcat
      721 ccatcctaaa ttatctccac atcaaattct gtgcattctc gtttgacatc agcggactcc
      781 tcctataaaa ttacgattag attgcatata tctagtgatt gtcgagttaa tgaaattatt
      841 gtatactata ttcaaaatat tgtcaacaaa aaaaatagaa aattttctga taatttgtga
      901 ttaaaaccaa taccatacct ctcccatcat tgaaaagcgc agtcattact taaaaagtca
      961 ctcgtttatg ttataatagg ctccataata tccatagggg atttacccac tacaaatatt
     1021 atagagccga ttttcattga gtagcaatcc tgtcatcctt ggcaggattt ttctatttca
     1081 aaaaagcaag tcgattgacc tgcttctgct ttacttttct tgtgctaatg ttttaaaatc
     1141 tttgtctaat atgggttgca cttctatttg attggcgtct agttggtggt aaaaagatgg
     1201 gggtaatacc tctaagaatt ttgcatagtc caagcgagct atggattcat agtcttctgg
     1261 tttagagccg tctcctgtga tttgaaccaa gtcaatctcc cactggactt ccttatccac
     1321 caattgctca atataggctg caggaacaaa cggttctctc ggtaaaactg tcttgactcc
     1381 ttgagctaga tggtaaacgc catgcacttt tccttctcca tcctgataga aggaaactct
     1441 cgcaaagtag gcatctgtct cttgaaccgc acgcacacgc gcttcaaact gagccaagcc
     1501 atctgcctct aaaaagcttt tcaagttctc acgactaaag aaaataggat taaaaattcc
     1561 ttggcaaatc gtaaaacgag ctacagggtc tgctagatag gcttgaatac ttgcttggaa
     1621 ataagcttca aaatcaaaac catctagccc ttcaatatca gtccctgtat aagcaagcaa
     1681 agcatctaaa aatgacttga taatctgcca gtctgtcttt gttagtaggt caggaagatc
     1741 gacacggtaa gccttttgac catcagcata actgacctta aagagaaatt gtgattgccc
     1801 cactatcgca cactcaatat actcgagacg attaagaggc tgacggagat agactacatc
     1861 atagctatgt gactccaaac cgtctagcaa ggccaagatc gacttggcat tcaaaatctc
     1921 ttgttctcct aaaatgctct gtttatttgg aataaaaaat gttttcgcca taactggact
     1981 cctttgaaat cgtttacata cagtatacct tatttttctg aaagatacaa aaacaaactt
     2041 tatattcttc gaaaatctct tcaaaccgcg tcagcttcgc cttgccgtag atatatgtaa
     2101 ctgacttcgt cagtcttatc tacaacctca aagcagtgct ttgagcagcc tgcggctagc
     2161 ttcctagttt gctctttgat tttcattgag tattagattt ttgagtacaa agcatagaaa
     2221 aacagcccct aaggactgtt tgatcttata cagtagctgc ctgatccaag ctttcaccga
     2281 tagcggctag gcgctcaatc acttcagctt gtgtcaattc attttttgaa acatagcggt
     2341 tacgtgggtg aacacggcac tcgtgtgagc atccacgaag gtacttgtct tcattttctt
     2401 ctgatgtcaa gatacgacgg ttacagaagg gatttccaca gttgacataa cgttcacatg
     2461 gtgttccatc aaaccagtct ttccctacga tggttgggtt gacatggttg acatcaacgg
     2521 caatacgctc gtcaaagacg tacattttcc catcccaaag ctcaccttga acttctgggt
     2581 ctttaccgta agttgcgatt cctccgtgca attggccaac atctttgtaa ccttcacgaa
     2641 ccatccagcc tgagaatttc tcacagcgaa caccacctgt acagtaaacc acgacacgct
     2701 tgtccatgaa tttttctttg ttgtcacgga cccattgtgg taactcacgg aagttgcgaa
     2761 tatctgggcg aatagcccca cggaaatgtc ctaggtcgta ctcataatcg ttacgtgtgt
     2821 caaggacaac ggtatcttta tcaagaagcg cttctttgaa ctcttttgga gacaagtaag
     2881 cacctgttgt ttcaagtggg ttgatgtcat tgtcaaagtc gttgtcttcc aaaccaaggt
     2941 ggacaatttc tttcttgtag cgaacaaaca tcttcttgaa ggcttgttca ctttcttcgt
     3001 caatcttgaa ccagagatct tccattcctg gaaggctgtg aacgtagtcc atgtattttt
     3061 gagttgtttc atagtcacct gaaactgttc cgttaattcc ctcgtcagcg actaggatac
     3121 ggcctttaag gccgattgat ttacagaaag ccaagtggtc tgcagcaaat tgctctgcat
     3181 tttcaattgg agtataaagg tagtaaagta agacacgaat atcttttgcc ataagatttg
     3241 ttctcttttc tattcttaaa ttttctaaat tttttattta tccatactag tatacctgct
     3301 tgagaaaacg aatgcaattt atttgactgg aaattgtaga ggattctcga aatttcctga
     3361 atggttaaaa taaggacaag agaaaacatg gatatctata tccttgtgcc aaaaaaacca
     3421 ctgccctccc cagaccaacc tgaggaaagc agtgattctt attttaggag ttaggaatga
     3481 atacacgaaa tcaatttagc tgattatttt ttgtttttca agaattcatc gtattgtttt
     3541 tgcatttcgt tcaatacttt ttcgtaggca ccttcagatt tcaatttttc catcaattct
     3601 ggaatagctt tatctgggtc tacagtacca gtgttgatag ctgtatcaaa ttgttgcatt
     3661 gtgttagcaa tagctgagat ttcagatttc acattgtcag tattgaagat aaatccaagc
     3721 gctggagatt ctttagcttc tgccaattct ttcttagaat tttcgatttg ttggtctgta
     3781 acgttttcgt tgatgtaaag gatccagttg ttaccagtgt tccatccacc catgtgagtg
     3841 tttcctttgt agccatcaag aacgcgaaca cggttttctt taccttcaat tttttcccag
     3901 ttcttgcctt ctggaccgta aacaagaccg ttcaagagtt ctggattcgt attcaagagg
     3961 ttcaagattt ccattgattt ttctttgttc ttagagttgt ttgagatgac aaagttagca
     4021 acttgtgttg tttggttttt cttgatgaag ttagtaattg gtttgatttg gatatctttg
     4081 ttggcaacac gtgaaagcaa gctgttaccg tagtcagctg gtcctactgt ttcttcacga
     4141 acgaaccaag tatcttgttg aaggtcaaag gaagtatcgc ttgttgcgac gtcttttgga
     4201 atgtagccag cttcatagaa tttgtgaaga gtcttcaagt gttctttgaa acgaggcact
     4261 tcgtaacggt ttacaacttt agtagtatcg ccttcaaggt cgataacgaa tggaagaccg
     4321 tttgctactg ggtagtcaaa gttatcagat gggatgaaaa ctttaccaat agcaaatggt
     4381 actacgtctg gagctttttc tttaatttgt ttcaagactg gctcaagagt ttcgtaagaa
     4441 gtaacacctg aaatatcgat accatattta gcaaggagag ttccgttgaa ggcaaagttt
     4501 tgagatgatg caacgttggc tgcaactgga acagcgtaaa tcttaccatt tacagtatta
     4561 cccttgatgt aagctgggtc aagtgctttg taaaggtctt taccttcttt tttgtacaat
     4621 tctgtcaagt cagcgtaagc acctttttga gcatttacaa tatagttatc tgcaaaggca
     4681 atatcatagt tttcaccaga tgatgtgata actgacattt tcttaccata gtcaccccag
     4741 ccaaggtatt ggatatccaa tttggcacca actttttctt caatgatttt gttggcattt
     4801 gctaacaatt catccaagtt gtctggtttg tcaccgattt ggtacatttt gataacaggt
     4861 ttgtcacctg aatcagcagc ttttttgctg ttacctgtca aatttccaca agcagcaaga
     4921 cctgcagcca aagcgactac actagcagat gcaaaagcat attttttcca gtttttcatg
     4981 ataaaaactc ctttttttat ttttaaactt ataaacaatg taatgatctt atactcaatg
     5041 aaaatcaaag agcaaactag aaa
//
LOCUS       DAVAAL010000124         4951 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVAAL010000124 DAVAAL010000000
VERSION     DAVAAL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.126"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            287..2170
                     /locus_tag="VQM60_001764"
     CDS             287..2170
                     /locus_tag="VQM60_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET1081596.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVVHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVILLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            2319..3206
                     /locus_tag="VQM60_001765"
     CDS             2319..3206
                     /locus_tag="VQM60_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET1081597.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGENIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(3526..4143)
                     /locus_tag="VQM60_001766"
     CDS             complement(3526..4143)
                     /locus_tag="VQM60_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET1081598.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQVGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(4558..4777)
                     /locus_tag="VQM60_001767"
                     /pseudo
     CDS             complement(4558..4777)
                     /locus_tag="VQM60_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1468 a          950 c         1062 g         1471 t
ORIGIN      
        1 aaacgattat cataaaataa atacaggaaa tcaatcaaat ttttacgaaa atgtgaaaga
       61 taatgaaata aaatactttt taaccaaggt ttcaaactta tttttcaagg aattcctgat
      121 gaaacagtca aaaacaatca atctaaagct tgcccactaa gtggatagaa tattttttct
      181 gcaaaacgct tgacatattt ttaaaacatg ataaaataat agtcgtaagg gcgaataaaa
      241 tcgctttcaa acaagaacaa aatgttatat aaggagattt ttgcaaatga acaatcagga
      301 aattgcaaaa aaagtcatcg atgccttggg cggacgtgaa aatgtcaata gtgttgtcca
      361 ctgtgcgact cgtctacgtg tcatggtcaa agatgaagag aaaatcaata aagaagtgat
      421 tgagaacttg gaaaaagttc aaggtgcttt ctttaactca gggcaatacc aaattatctt
      481 tggtacaggt acagttaaca aaatgtacga tgaagttgtt gtacttggct taccaacatc
      541 atctaaggat gacatgaaag cagaagttgc taaacaaggg aactggttcc aacgtgctat
      601 ccgtactttc ggtgatgttt tcgttccaat catcccagtt atcgtagcga caggtctctt
      661 catgggtgtg cgtggtcttt tcaacgctct tgaaatgcca cttccaggtg actttgcaac
      721 ttacacacaa atcttgacag atacagcctt catcatcttg ccaggtttgg ttgtgtggtc
      781 aaccttccgt gtatttggtg gaaatcctgc cgttggtatc gttcttggta tgatgcttgt
      841 ctctggctca cttccaaacg cttgggcagt tgctcaaggt ggtgaagtaa cagcgatgaa
      901 cttctttggt ttcatccctg ttgttggttt gcaaggttcc gttcttccag ccttcatcat
      961 cggggttgtc ggagctaaat ttgaaaaagc tgtccgcaag gttgttccag atgtcattga
     1021 cctattggta acaccattcg tgatactttt ggtcatgtct atccttggac tctttgtcat
     1081 tggaccagtt ttccacgttg ttgaaaacta catccttatt gctacaaaag cgattcttag
     1141 catgccattt ggtcttggtg gtttcttgat tggtggggtt caccaattga tcgtcgtgtc
     1201 aggtgtgcac cacatcttca acttgcttga agtgcaatta cttgctgctg accatgctaa
     1261 cccattcaac gctatcatca cagctgctat gacagctcaa ggtgctgcta ctgttgcggt
     1321 tggtgttaaa acaaaaaatc caaaactgaa aacacttgct ttcccggctg ctctttctgc
     1381 cttcctaggt attacagagc ctgctatctt cggggtgaac ttgcgcttcc gtaaaccatt
     1441 cttcctttca ttgattgctg gtgcaatcgg tggtggattg gcttctatcc ttggacttgc
     1501 tggtactggt aatggtatca ccatcatccc tggtacaatg ctttatgttg gtaacggaca
     1561 acttccacaa taccttctta tggtagctgt atcatttgcc cttggttttg ctcttactta
     1621 catgtttggt tacgaagatg aagtagacgc aactgcagct gcaaaacaag ctgaagtggc
     1681 tgaagaaaaa gaagaagttg cgccagcagc tcttcaaaat gaaacacttg taactcctat
     1741 cgtcggtgat gttgtcgctc ttgctgatgt caatgaccca gtcttctcaa gtggagctat
     1801 gggacaaggt atcgctgtga aaccaagcca aggcgtggtc tatgcaccag ctgatgctga
     1861 agtttcaatt gcctttccaa cagggcacgc ttttggtttg aaaacaagaa atggtgctga
     1921 agttttgatt cacgttggta ttgacactgt atctatgaac ggtgacggtt ttgaagcaaa
     1981 agttgctcaa ggtaataagg tgaaagctgg tgatgttctt ggaacatttg actcaaacaa
     2041 aatcgctgca gctggacttg atgatacaac aatggttatc gttacaaata cagctgacta
     2101 cgcttcagta gctccagtcg caacaggttc agttgcgaag ggggatgctg tgatcgaagt
     2161 gaaaatctaa tcaatcctct ctaatgtgaa aacgaacaaa tgacatgttt gttcgttttt
     2221 attagatagt aagatttaca gaggaaaatc taaaaaatag agacatttag actttcgaag
     2281 tatgctataa taaagaaaat aaaaacaaga ggtttatcat gacaaaatta tatggaagct
     2341 tggaagcggg cggtacaaag tttgtctgtg ctgtcggtga tgaaaacttt aacgtcgtag
     2401 aaaaaacaca atttccaaca acaactccaa tcgaaacaat cgataaaacc attgagttct
     2461 tctcaaaatt cgataacctt gctggtcttg cagttggttc atttgggccg attgatattg
     2521 acaaaaactc aaaaacttat ggctttatca cgacgactcc aaaaccaaac tgggcaaatg
     2581 tggacttgct tggtgccctt cgtcgcgccc taaacgtgcc aatgtacttc acaacagacg
     2641 taaacagctc tgcttacggt gaagtggttg cccgtaacaa tgctggtggt cgtatcgaaa
     2701 acttggttta ctacacaatc ggtacaggta tcggtgcagg tgtcatccaa cgtggtgagt
     2761 ttatcggtgg tgtgggtcac cctgaaatgg gtcattatta tgttgctaga cacccaatgg
     2821 atattgaaaa agagtttaag ggtgtttgtc ctttccataa gggatgtcta gaaggttatg
     2881 cagctggtcc aagtttggaa gctcgtacag gtgtacgtgg ggaaaatatt gaactcaaca
     2941 accctgtttg ggatgttcaa gcctactata tcgctcaagc tgcggttaat gcgacagtga
     3001 ctttccgccc agacgtgatt gtctttggtg gaggggtcat ggctcaacaa catatgctgg
     3061 accgtgtccg tgagaaattt acatctcttc ttaatggtta cctaccagta ccagatgtgc
     3121 gtgactatat cgtgacgcca gcagtcgcag gaaatggttc tgccacactt gggaactttg
     3181 ttcttgcaaa agaagtttca aaataaagca caaaatccgc ttgggaaacc aaacggattt
     3241 tgtggttgaa aacaatattt ttgagccttt ccttaagtga ttacggacgg tagcgacttt
     3301 cttcgaaatt ccatacctaa actttgatcc taatgtctca aagtttccga acacctgaaa
     3361 ccattagatt tcaggtgttt ctatcacggc ggaaaggcta ggtaagtcct cgcctcacta
     3421 tcgaaaaata tgcataattt ccataccaaa gtatatttag tatcaagcta tagttaatct
     3481 acgctttaaa aatccgcttg ggaaaccaaa cggatttttt acgtgtcaaa gcatttgcca
     3541 gaaaaagtca ataatatagg tcagaccgta ggtataaacc acgagggtaa agggtttggt
     3601 cagaatgatg atggtcatgt agcgcttgaa actcatcttg gtcagggcag ccagcataca
     3661 gagaaagtca gctgggctaa tgggccaaat catcataaaa ataaagaagc ggtcaaaacg
     3721 attgccctta tctagccagc cgatgtactt gtcgtaggtg cgcttgctga cgacagattg
     3781 gacaaaggca gctccgtata ggcgcaccag ataaaagata atggcacagc caatcacgat
     3841 gccgatatag ttgtagatag tcccgatgat gtgcccgtag ataaagaccc cagccaccga
     3901 ggtcaaggcc cctggaatga tagggacgac agtctgtaaa atctgtaaaa agataaagag
     3961 aggtggaccc cagatgccta cctgctggat aaaggcagag agggtttcct tggattgtaa
     4021 aatcccagcc tgataagccc aaatacagaa aatcaaactc ccaaccccac cgacaatcga
     4081 tgagatattg ataactcgct gcagaagggg agaaacagct ttcatttgtt tatcctgtga
     4141 catgtaaact cctcatagat agtatatttc tactatacca tattttggag gagaaagggg
     4201 gaatgagtct tttttgataa ggaatagaaa gtaaatagcc cttcgagact tagaataggt
     4261 ctgtcaacta aaggttatat gcattctagg tttcgaataa tcgcaattcc ataaatttaa
     4321 aagcattcta agtttcgaat agcttatctg agataatttt gaggcattcg cgctttcgaa
     4381 tagtcatttt ccctaaagtt tagatgtatt ctgaagctag aaagactaaa atctaaaagt
     4441 agtaagagtc tctctctgat ggacgtaatt tatggctagt atgaatcctt tgaatcgtta
     4501 gttaatttta atattaatca gttctgaaat aattactaaa tatagttaga tggtgaatta
     4561 attattgcta aatttcaagt tcaagttagt catccagaag tcttctctgg gggacttgta
     4621 gttcaagcat tttttaggat agttgttaat ccacttttcg atgaatgcga cttcctttgt
     4681 agtcgttttc ttagttccct taggcttagg taaccatcta tgaatgagcc tgttgtgatt
     4741 ttcattagat cctatagggg tgtgcataat agatatagca gattgaaact agaatagtat
     4801 acatctactt ctaaaacatt gttagaaatc gatttgactg tcccgatcta tttgtcctgt
     4861 ttttatttca ttttactata taatccttag aaaacatatc ggacaagcgg ttgaattccg
     4921 ttccgtccat tagtaaataa aggattactc c
//
LOCUS       DAVAAL010000125         4923 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVAAL010000125 DAVAAL010000000
VERSION     DAVAAL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.127"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..821)
                     /locus_tag="VQM60_001768"
     CDS             complement(<1..821)
                     /locus_tag="VQM60_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET1081599.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVID"
     misc_binding    complement(934..1151)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1234..3930)
                     /gene="ppc"
                     /locus_tag="VQM60_001769"
     CDS             complement(1234..3930)
                     /gene="ppc"
                     /locus_tag="VQM60_001769"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET1081600.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITHKKSDTNTPNRYEAIMDQVVDRS
                     YDVYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(3950..>4923)
                     /locus_tag="VQM60_001770"
     CDS             complement(3950..>4923)
                     /locus_tag="VQM60_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET1081601.1"
                     /translation="LLLFLARFIGISVNGAYGWISVAGVTIQPAEYLKIIIIWYLAHR
                     FSKQQEEIATYDFQVLTQNQWLPRAFNDWRFVLLVLIGSLGIFPDLGNATILVLVSLI
                     MYTVSGIAYRWFSTILALVSATSVFVLTTISLIGVETFSKIPVFGYVAKRFSAFFNPF
                     ADRADAGHQLANSYFAMVNGGWFGLGLGNSIEKRGYLPEAHTDFVFSIVIEEFGFVGA
                     SLILALLFFMILRIILVGIRAENPFNAMVALGVGGMMLVQVFVNIGGISGLIPSTGVT
                     FPFLSQGGNSLLVLSVAVAFVLNIDASEKRAKLYRELENQPMNLLLK"
BASE COUNT         1507 a         1043 c          852 g         1521 t
ORIGIN      
        1 tggtcaatta caactgatgc caaaccacct acttctacca tagctgtcgc actgggataa
       61 attggtttct tagaactttg attgctagag acaaccgttg gaaatcctga tgcaatggtg
      121 ttatcaattg gaacccaaat agcatctacc ttgctagtca taacagtgac agttgaggca
      181 atttcatttg ttgaaggaac tgcaaatgtt tccactgtca gacctgcttt ttcagcataa
      241 gccttaaatt cttcgacctg tgtttttgaa ttgtcttcgc tacttgagta aagagctccg
      301 attgttttca cattcggtgt cagagccttg atgagttcaa cttgttgttg agctggattg
      361 tggtcagata cccctgtaac gttgccacct ggttttttca aatctttaac caagttagca
      421 ccaattgggt ctgtaatagc ggccatgata accggtaggt cttttgttgc actagccaac
      481 ccttgggctg ctggtgttgc gataccaacc acaaggtcat tcccatttgc aaccaattgt
      541 ttactcattg tcgcaacctt actttggtca ccttctgagt tcataaaatc aattttaact
      601 tgatcatctt tatatccttc ttctgcaagt ccatcttgga tccctttata aatcaaatca
      661 agggatggat ggctcacaaa ttgaagcaca ccaatcttgg ttattttctt ctcatcctta
      721 ttattctgag cttctgattt attcattgaa gaataaatca agcttcctgc tactaagact
      781 gctaatgcag caataattcc aattaaacgt ttatttttca ttctgtttct cctttttatt
      841 tcctttaaca tatttcactt atctaaacaa gcgacgccat tgttttcttt ccatactaac
      901 ctccatcaaa aaaatcctca cacaaaaaac ttgtgtgagg acgtcgatgc gcggtaccac
      961 ctcaattata gggaatttcc ctatcgctca gtctcgcaat aacgagatgc actgtaaggt
     1021 gtgcttaccg aatttttata atttcaaatt ctaaatagca ttcagcccaa ttttcatcat
     1081 tgccacttat ctgttttcag ctaccacaga ctctctaaaa agtttgtatg attacttttc
     1141 tgaatggtta aattatatca tttttaaact acttgtcaat agtctattag aatttttttg
     1201 atattcactt ttagcattca ctcttgaaaa ttatcaacct gaattacgca atcctgtcgc
     1261 aattccgttg atggtgatat gaatcaatcg ttcttgatca ctggacaatt ctccacgacg
     1321 ttggcgttta atcaactcta actgaatata gttgagaata ttaaagtaag gcatacggta
     1381 atccagacta gcttttagat atggattgtc agctaagagt tcgtcatgtc cttcaatagc
     1441 caagataacg ttcttagtaa cttgccattc atttaaaata gtctcataga tggccttaac
     1501 ttgctcgtct tcacaaagtt tagcatattc aaaagcaata ttcatatttg attttgacaa
     1561 aaccatatca acatttgaaa gaagcgattg gaagaaaggc caattttggt acatatctcg
     1621 taagatagca atattctctg gatttttatt gataaattcc ttgaagcttg aaccaacccc
     1681 gtaccatcca gggaacataa cacgactctg tgaccatgag aatacccaag ggatggcacg
     1741 caaaccaccg atttcagtaa tagtcttacg agcggctgga cgagaaccaa tattaaaact
     1801 tgaaatagcc ttgattggac ttgactcgaa gaaataatca tagaaatgct cattaccaaa
     1861 gaccaaatca cggtagacat cgtaactacg gtccactact tgatccataa tggcttcata
     1921 acgatttggg gtattggtat cactcttctt atgagtaatc atacggttaa tagctgcaga
     1981 tactagcatt tcaaggttat agtaagcggc gtctttgtta ccgtatttat tcccaattac
     2041 ttcaccctgc tccgtcaaac ggatacgatc cttgatagac ttgagcggtt gagatgtaat
     2101 ggcttcatag gttggcccac caccacgacc gacagtacca ccacgaccat ggaagaaggt
     2161 aaccttaacg ccaaattcat ctccaatagc agtcaattgt tgttgagcct tgtagagggt
     2221 ccaacatgat gacaagtaac cgccatcttt attactgtca gagtagccaa gcatgatttc
     2281 ttggtagtta tttcgtgagt caatccattt tttggcaagg ctaagagaaa gatattttct
     2341 cattgtttcc tctgaatgat ccaagtcttc aattgtttca aaaaggggaa caatctgaac
     2401 acgcgccctt tccgtatcca ccagtcctac ttcttttaac agaatagcta attctagcat
     2461 atcagaaagg ctggttgcat gtgaaatgat ggtctgacgg atgacatcat ctcccaactt
     2521 atctttcaaa acacgagccg tcttaaaaat agctaattct tttgctaata attctgattt
     2581 ttctgcgtga gtcgcagaaa gaattcgggg atcttcttct aattctttca agagaaggtc
     2641 acacttttct tcttcgctca actcgctata acgagaatga attcctgctg atttcaagag
     2701 ttctgccaca caggcttcat agacgctaga gtcttgtcgc atatcaattg atgctaagta
     2761 aaaaccaaat atctctactg cctgcaataa ttccacaaaa tcacctgaaa tcaaggactc
     2821 gcccttattt tctagtagag aatctcgaat ggcaatcaaa tccttgtaga aatcattggc
     2881 ggtttcataa cgagtcccaa cttcttcatc ctcaatcaga taggttttag ttgcctgaat
     2941 ttttgattga atatcaaaca aggcacgacg gtaaagctct ttttcgcggt aaatcgagtt
     3001 atccttggat tgacgagcca tttctctgac ttgcttgctg acgttgacaa tgctagttga
     3061 aagagaaaat tcacgataaa gttggtaaat ctttttatca tagtagttca tgatgacttc
     3121 acactgagtg agtgcagact gcttcaaggt ctttgctgta acaaatggat ttccatcacg
     3181 gtcaccacct atccacatac ccatggtgat tggtttagcc tgttttaaat tcagaccatg
     3241 cgcttgcgct aagcgcttat actccgtcgt caaatgaggt acagctttca aaaaggagct
     3301 gttataatat tccatagcat tcgtgatttc gttagtcact tttaattttt tctcacgaat
     3361 catgtctgtc tgcatgataa tttcgatgta acgacgcaaa tcattgtacc atttatcttt
     3421 attgatcaac cccaacttaa catcacggta tttacgcaaa agactatgaa tatgatttgt
     3481 taaatccaac atacttttgc gttgcacttg tgttggatgg gctgtcaaaa cagggacaac
     3541 attcaagtgt tctaggatct caacggcatt ttccttttct gctaccaatt taatcgttgt
     3601 agataattta cctaaatagt cctgatcaat attattttga tgattgattt cataagctaa
     3661 atccacatcc tctgaaatat taatcaaaag aggtaagata gagaaatagc gtgaaatata
     3721 gaccatttca tcatttgata agctagtcac tagacggttt agaccttgat aatcttcctg
     3781 cgttgataat tctttcaact gtattatttt ttcaaaggtc tctggggcaa gcatattttt
     3841 agtaatatct tccagtaatt ctgttagaat caagacttct tcttgcacaa cacttttatt
     3901 actataattt tctaattttt gaagagacat aaactatcct ttctttatcc tacttcaaca
     3961 gaaggttcat tggttgattt tccaattctc ggtacaactt agcgcgtttt tcactggcat
     4021 caatatttaa gacaaaggct actgccactg ataagactag aagactattt ccaccctggg
     4081 ataagaaagg gaaagtcact cctgtagatg gaatcaagcc cgaaatccct ccgatattga
     4141 caaatacctg aaccaacatc atccctccga caccgagtgc aaccatggca ttgaaaggat
     4201 tctccgctcg gataccgacc aagataatcc gcaaaatcat gaaaaacaag agagctaaaa
     4261 taagactggc accaacaaag ccaaattctt caatcacgat agaaaagaca aagtctgtat
     4321 gagcttctgg caaataacct cgtttttcaa tcgagtttcc aagacctaga ccaaaccaac
     4381 caccattgac catggcaaaa taagaattag ctaactggtg acctgcatca gcacgatcgg
     4441 caaaaggatt aaaaaaggca ctaaagcgct tggctacata gccaaatact ggaatttttg
     4501 aaaaggtctc aacaccgatt aggctgatag tggtcaagac aaagacagaa gtggcagata
     4561 cgagcgccag aatggttgaa aaccagcgat aagcgattcc actaactgta tacataatca
     4621 aggaaaccaa gactaaaata gtcgcatttc ctaaatcagg gaaaattccc aaacttccaa
     4681 tcagaactag gagaacgaat cgccaatcat taaaagcacg gggaagccat tgattttgag
     4741 tcaaaacttg aaaatcataa gtagctattt cttcttgctg tttggagaat cggtgagcta
     4801 aataccaaat aataatgatt tttaagtact cagctggctg aatagttact cctgcaaccg
     4861 aaatccaacc gtatgccccg tttactgaaa taccaataaa acgagccaag aacaataaaa
     4921 gca
//
LOCUS       DAVAAL010000126         4828 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVAAL010000126 DAVAAL010000000
VERSION     DAVAAL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.128"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(94..1842)
                     /locus_tag="VQM60_001771"
     CDS             complement(94..1842)
                     /locus_tag="VQM60_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081602.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRNLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKKSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(1844..3568)
                     /locus_tag="VQM60_001772"
     CDS             complement(1844..3568)
                     /locus_tag="VQM60_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081603.1"
                     /translation="MKYLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLIQLILGLYPVNKGRIDL
                     YLDGLSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            complement(3626..4564)
                     /gene="msrB"
                     /locus_tag="VQM60_001773"
     CDS             complement(3626..4564)
                     /gene="msrB"
                     /locus_tag="VQM60_001773"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET1081604.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
BASE COUNT         1431 a         1063 c          999 g         1335 t
ORIGIN      
        1 gagtattaca gtaaacaatt tttctagcta ttgtaacaaa aaagtcactc gaaggcaact
       61 tttttcgtcc ataaactttc aaaagagaat ggcttattca gaactaaaag ccgcagcttt
      121 ttgcatttgg taatacttac cctttctatc catgagttct tgatggttac catattcaac
      181 aatatcacca tctactaaga caagaattaa atccgcatcc tgaatggttg acaaacggtg
      241 agcaatgatg aagcttgtgc ggcccttcat gagttttgca aaggcatcct gtaccagcac
      301 ttctgtccgt gtatcaatgg aagaagttgc ctcgtctaag ataagaatct ttggaatagc
      361 cagaaagact cgggctatgg tcaagagctg agcttggccg acagagagag attctccagc
      421 attttccaac ttggtatcgt atccctgtgg caactgttgg atgaaaaagt ctgcattagc
      481 tgctttggca gcagcaatta cttgctctcg actggcttca ggattgccaa aggcaatatt
      541 atcatgaatg gtcccttgtg tgagccaggt ttcttgaagc accataccaa actgctgtct
      601 caatgatact cgtgtataat cataaatgga ttgcccatcc agcaagatat ctcccgagct
      661 aatgggataa aaacgcataa ggagattgat aagagttgat tttccagcac ctgtaggacc
      721 aacgatggct accttactac cagctggaat atcgatagac aagtccttaa tcaaaatttt
      781 ttcaggatgg tagccaaaag agacatgttt aaaggaaata gctcccttaa cttggtcact
      841 ggtcaagact tccttacctg tttcagccac ttcagggcta tctaagactc catagatacg
      901 ctctacgcaa gccagagcac tttgcaactc agctagcact gaagaaatat cgttaaaggg
      961 cttggtgtat tgctgaacat agttcaaaaa agtcactaaa cgaccgacgg tcaaggctga
     1021 acccatcatg atacgataag ctcctactcc agctaaaagg gcataaatga gtgcatttac
     1081 aaagcgagtc gaaggattga ccgttgaaga ataaaagatg gctgactgag aatagcctga
     1141 gtagttgtca tgagcctcac gcaatctttg gataaattct gtttgagcat tgaaggactg
     1201 gattatagtc tgctgactaa gcgattcttc aatcaactga gtctgaattc ccctcgtctc
     1261 tgtttgcttc tggaagagat gataggattt cttggcaata aagcgtgaaa tcaccatgga
     1321 cagtggcgtc aacagcaaga ctaagagagt catgaggaga tgaatttgga gcatggctag
     1381 aatactaacc aaaatcatca aaacaccaat gaaaaattgg ttaaaaatca tggtcaagcc
     1441 agctgccaac tgttcgatat ccgtggttac acgactgacc atctctccat tgccttgccg
     1501 atccgcaaaa gcaatcggta accgatggag tttatggatg attcgctctc gtaaatttct
     1561 ggtataagag aagattagac gattatagag gagaggattg gcccattgta ccagagtatt
     1621 tcctattacc accaagagca tctggagaaa aatctgccaa aaaactggtg atgaaccagc
     1681 cactaggact tggtcaatga cctgcccaat cagaataggt aggtaaattg ataagccaac
     1741 ttgggcaata gttcctagaa aggctaggaa aaggaggaaa ggatggcttg ctaaatctac
     1801 ggctaaacgt ttgagcgtct ggtttacagt ttgtcgtttc attctagtcc tcctttccat
     1861 gttgggatgc attgatttca caatagactt ggctggattt catcaagtca tcgtgcttgc
     1921 caacagctag caactcacct ttttccaaga ggagaatctg gtccgccatc tgtaaagttg
     1981 aggttcgttg agagatcaaa attaagctcg tgtttggaaa attttctcta atagctttca
     2041 agagcttgga ctctgtaatg gtatccagtg ccgaggttgc atcatctagg atgagaaacg
     2101 gagcctggcg caagactgct cgggcgatag acaatctttg tttttgtcca cctgagaaat
     2161 ttcgcccccc tgcctcaact agagcatcca agagtccttc cttttcactg acaaaatcct
     2221 tagcttgcgc aatctccaag gcctgccaga gttcctggtc agatacttct tgattgaaac
     2281 ctagagtcaa gttggaacga atagttccct taaagagttc gaccttttgt ggcacatagg
     2341 caatccaaga tctccattgc tccaaattaa gaggactcaa tccatctaga taaaggtcaa
     2401 ttcttccctt gtttactgga taaagtccaa gtatgagttg aatcaagctt gatttaccag
     2461 aaccagttcc cccgatgata cctagaattt gtccttgagt catatcaaag gaaatgtctc
     2521 tcagagaagg ctgggccgca tcaggatagg taaaggtcaa ttcttggact tgtaaaacct
     2581 tatctctggt agcttgcttt tgttctaact ctgaatggat atcctctgga gcctcaacaa
     2641 agacttcctc gattcgcttg gctgagatat aggactggtt gagggaattg atcaacatgg
     2701 ctagcttgac caattccacc aaaatctgta agaggtaatt gataagagca atgagagcac
     2761 cttgactgag cactcctcct tgaattgaaa tatagccttg ccagataata acgagaagag
     2821 ttccattgac aatcagatag gtcagaggtg ttaataaact agaccagaaa cctgtctttt
     2881 cttgtaatct agcataaact tggttaaggg tttgaaaaat ctgtaactct cgtttttctt
     2941 gaccaaaagc acgaataacc cgcatccctt gcaattgctg gcgcgtttcc tgaaccagtt
     3001 ggtccgtttt ctttctgaga ctactgtaga gaggattgac caatcgagat aaccctacaa
     3061 ttacaatggt caaaatggca accatgacta agaaccagaa agtcaactca gctgagattc
     3121 gataagccat aaaaatggca ccaaaaacga taatgggcgc tcgcaaaaag agacgcagga
     3181 attgattgat accagtctga atctggtagg tatccgaagt caagcgagtg accaaactag
     3241 aagttgtcag acggtctctg ctgtccttgg gcaaggaaag aatatgacga taaagatcgt
     3301 ttgtcaattc cttagcaaag cctactgctg cctttgctga gtaaaattga gctatcaagg
     3361 ccactaaaac gccaattact gcaaagataa ggagcaggcc aatctgcatc cagagatgac
     3421 cttgatctcc ctgaggtaaa gattggtcaa caatcccagc aatcaccatg ggaaccaaga
     3481 gctcaaaaac agcttctaac agcttgaaca agggggctaa aattgattcc ttgatgtagg
     3541 gtttgaagta agataatagg tatttcataa atcccttcta ttcatattct agaaatgaag
     3601 aaagtgggaa gccccactct ctgttttatt tgtttaagta aggcaataga tagccatatc
     3661 ctgctttttc catctcatcc ttggccacaa agcgtaaaga agcagaattg atacagtaac
     3721 ggaggccgcc taactcccgc ggtccatctg tgaaaacatg acccaagtga gcactgcctg
     3781 aacgagaacg aacttcaatt cgctccattc catggctcag atccttgtaa taatgaatca
     3841 actctttgga aatcggacgg ctaaaacttg gccaaccaca acctgaagca aacttatcct
     3901 tggcaaaaaa gagtggctca cctgtcgtaa tatctacata aatcccctct tcaaaggttt
     3961 ggtcataggc attggtaaat ggagcctctg tagcagcttc ttgtgtgaca cgataagact
     4021 cttcagatag actggccttc aacacctctt gactaggctt ttcatagttt gctgcatcaa
     4081 tcaatggctt atcagcatcg gtcacatcga tatgacagta acctgaagga ttcttcctga
     4141 gataatcttg gtggtagtct tcagccagaa tgtagtggcg taattgctcc acttctactg
     4201 caatctttcg acccagcatg cgttcctgct cttgtaccac tgtgtagata gctggcaaat
     4261 ctgcttcatc ctgataataa atcccagttc gatattggcg accacggtca ttcccttgtt
     4321 gattgataga taaaggatcg ataactcgga aataataaag taaaatctct ctgagtgaca
     4381 cttccttctc atcgtaaatc acttggaccg tttctgcatg gtctgtttcc ttgagcaact
     4441 ggtaattggt cgtttcgact tgaccattag cgtagccaac actggtttct agcactccag
     4501 aaatgcgtga aaaatattcc tctaggcccc aaaaacaacc acctgctaga taaatttctg
     4561 ccatttttat ttctccttta tcacgttttt aactaatact cttcgaaaat ctcttcaaac
     4621 cacgtcagcg tcaccttacc gtaggtatgg ttactgactt cgtcagttct atctgcaacc
     4681 tcaaaacagt gtcttgagct gacgtcgtca gttctatcca caacctcaaa acactgtttt
     4741 gagctgacgt cgtcagtctt atctacaacc tcaaagcagt gctttgagca acctgcggct
     4801 agcttcctag tttgctcttt gattttca
//
LOCUS       DAVAAL010000127         4778 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVAAL010000127 DAVAAL010000000
VERSION     DAVAAL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.129"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..345
                     /locus_tag="VQM60_001774"
     CDS             <1..345
                     /locus_tag="VQM60_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET1081605.1"
                     /translation="LTADKKLDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDW
                     LSQAGVTRILTRAGVSGDSLEKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQL
                     GVTQLHGTKVVF"
     gene            389..1045
                     /locus_tag="VQM60_001775"
     CDS             389..1045
                     /locus_tag="VQM60_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET1081606.1"
                     /translation="MFEIFKSYQFNQEKVHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            1064..1696
                     /locus_tag="VQM60_001776"
     CDS             1064..1696
                     /locus_tag="VQM60_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET1081607.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNICQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            1724..2158
                     /locus_tag="VQM60_001777"
     CDS             1724..2158
                     /locus_tag="VQM60_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HET1081608.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            2170..3207
                     /gene="leuB"
                     /locus_tag="VQM60_001778"
     CDS             2170..3207
                     /gene="leuB"
                     /locus_tag="VQM60_001778"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET1081609.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLVAIGSPQYDGVAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERIAGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFSDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSV
                     PDIAGQGIANPISMILSVVMMLRDSFGRYEDTERIKRAVETSLAAGILTRDIGGQAST
                     KEMMEAIIARL"
     gene            3204..3473
                     /locus_tag="VQM60_001779"
     CDS             3204..3473
                     /locus_tag="VQM60_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET1081610.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            3477..3836
                     /gene="leuD"
                     /locus_tag="VQM60_001780"
     CDS             3477..3836
                     /gene="leuD"
                     /locus_tag="VQM60_001780"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET1081611.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSKWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            3998..4621
                     /locus_tag="VQM60_001781"
                     /pseudo
     CDS             3998..4621
                     /locus_tag="VQM60_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         1480 a          852 c         1090 g         1356 t
ORIGIN      
        1 ttaactgctg ataaaaagtt ggataagcct aatctggaaa agttaattgc tgcatcaaaa
       61 ggaatggaaa ttgtctttca catggccttt gatgaactaa gtgatgaaga tcaaccggaa
      121 gctattgact ggctcagtca agccggtgtc actcgtatcc taactcgtgc tggtgtgtct
      181 ggcgactcct tagaaaaacg ttttgttcac tatcacagaa ttttggagta cgctaaaggt
      241 aaaattgaaa ttctaccagg tggggggatt gaccttgaca accgtcaaac ctttatcgac
      301 cagttggggg taacacaatt gcatggtact aaggttgttt tttaaaaaat agaaaggaac
      361 tgctagcttt gggtagcagt tttcacttat gtttgaaatt tttaaatcct atcaatttaa
      421 tcaagaaaag gttcatgatt atggttttat agaaaatagc gaagtctgga catatagttg
      481 ccagattttg caaggtgact ttgtcatgac tgtgtccatc actgctgata atgtgaactt
      541 tcaagtcttt gaccaagaga ctggtgacct ctatcctcac gttcatatgg aaagcatgag
      601 gggaagtttt gtcggaaatg tccgtgaggc ttgtctggag attctttacc agattcggaa
      661 ggcttgtttt gatgtgcaag attttatctg tcatcagact aagcgtatca tgactcaagt
      721 tcaggaaaag tatggaaacc agttggagta tctgtgggaa aaatcgcctg atacagctgt
      781 attgcgccat gaaggcaatc aaaagtggta tgccgtcttg atgaaaatct cttggaataa
      841 gctggaaaag ggcagagaag gacaagtgga agcagtcaac ctcaagcatg accaagtagc
      901 taatttgctt tcacaaaagg ggatttatcc agccttccat atgagcaagc gctactggat
      961 tagtgtgtcc cttgatgata ctttatcaga tgaagaagta ctggaattga tagaaaaaag
     1021 ttggaactta acctctaaaa aatgaaatat tttaataatt ttcatgaact ttcaattagc
     1081 taaatattct ttactgaaga aattttcaga aaatatagga tttaccacac cagaggaatg
     1141 tggtgccatc ttcaaatacc tgattgagaa tgtcaagacg gatcgtcaga tcatctattc
     1201 gcctcactgt catgatgacc tcggaatggc agtggcaaat agccttgctg ctgtcaagaa
     1261 tggtgcagga cgtgttgaag ggactatcaa tggtattagg gagcgagctg aaaatgctgc
     1321 tttggaagaa attgcagtgg ctctcaatat ttgccaagat tactaccaag tagaaaccag
     1381 tattgtccta aatgagacca tcaatacgtc agaaatggtt tctcgcttct ctggtattcc
     1441 agttcctaaa aacaaagccg tcgttggtgg caataccttc tcccacgaat ctggtattca
     1501 ccaagatgga gtccttaaaa atcctctcac ttatgagatc atcacacctg aattggttgg
     1561 tgttaagatt ccgcttggaa aattatctgg tcgccatgct tttgttgaga aactgagaga
     1621 attggcccta gattttacag aagaggatat caaaccactc tttgctaagt tcaaggcact
     1681 ggccgataag aagtaagaaa tcacagatgc agatactcga gctttggtag ctggaaccat
     1741 ggttgaaaat ccagaaggct tccactttga tgatttacaa cttcaaactc atgcagataa
     1801 tgacattgaa gcgctcgtta gcctagccaa tatggatggt gagaaagtcg aatttaatgc
     1861 gacagggcaa ggttccgttg aagcaatctt taacgctatc gataagttct ttaaccaatc
     1921 tgttcgtttg gtgtcctaca ctatcaatgc ggtaacagat ggaatcgatg cccaggatcg
     1981 ggttttggtc actgttgaaa acagagatac agaaaccatc tttaatgcag cagggcttga
     2041 ttttgatgtg ttgaaggctt ctgctattgc ctatataaac gctaatacct ttgttcaaaa
     2101 agagaatgca ggtgagatgg gacgcagtgt ttcttaccac gatatgccta gtgtgtaaag
     2161 gagaaggcta tggcaaagaa aatagtagct ctagtaggag acggaattgg cccagaaatc
     2221 atggaggctg gtttagaagt tctggaggct ctagctgaaa aaacaggttt tgactatgag
     2281 attgacagac gaccgttcgg aggtgcagat attgatgcag caggacctcc cttacctgat
     2341 gaaaccctta aggcaagtag ggaagcagat gctatcctac tagtagctat cggtagtcct
     2401 cagtatgatg gagtagcggt tcgccctgaa caaggcctga tggctctccg taaggaactc
     2461 aatctttacg ctaatattcg tcctgtaaaa atctttgaca gtctcaagta tttgtcacca
     2521 ctcaaaccgg aacgaattgc tggtgtagac tttgtcgtgg tgcgtgaatt gacaggcgag
     2581 atttactttg gagatcatat tcttgaagag cgcaaagcgc gtgatatcaa cgactatagc
     2641 tatgaggaag tggagcggat tattcgcaaa gcctttgaaa ttgcaagaaa tcgcagaaaa
     2701 atccttacta gtatcgataa gcaaaatgtt ctagcgacct caaaactctg gcggaaagta
     2761 gctgaggaag tcgcacagga tttctcagat gtaaccttgg aacaccagct ggtagactca
     2821 gctgctatgc ttatgattac caatcctgct aagtttgatg ttattgtaac ggagaatctt
     2881 tttggagata ttttatctga tgaatcaagc gtcttatctg gtacacttgg ggttatgcca
     2941 tcagccagtc attctgaaaa tggaccaagt ctctatgaac ctattcacgg ttcagtacct
     3001 gatattgcag gtcaaggaat tgccaatcct atttccatga ttttatcagt tgtcatgatg
     3061 ttgagagata gtttcggacg ttatgaggat acagagcgta tcaaacgtgc tgttgagaca
     3121 agtctggcgg caggaatttt aacgagagat ataggaggtc aggcttcaac aaaggaaatg
     3181 atggaagcta ttattgcaag gttatgaagt tagacgaaaa aattactcta gtccttttga
     3241 tttggaatgt catcattttc ttgatttatg gcattgacaa attcaaggca agaagaagag
     3301 cttggcgcat cccagagaaa atcttactca ttttagccct tacttgtggt ggttttgggg
     3361 cctggttagc tggaatcact tttcaccata agactcgaaa atggtacttt aaaacagttt
     3421 ggtttcttgg gatggtgacc acactagtag ccttatattt tatttggagg taatggatgg
     3481 cagggtcttc gagggaacac gctgcttggg ctctagcgga ctatggtttt aaggtcgtga
     3541 ttgcaggatc tttcggtgac attcattaca ataatgaact caataatggc atgttgccaa
     3601 tcgttcagcc tagagaggtt agagagaaac tagcccagct aaaaccaacc gaccaggtaa
     3661 ctgtggactt ggaacaacaa aaaatcatct caccagttga agaattcacc ttcgagatag
     3721 atagcaagtg gaaacataaa ctcctaaata gtttggatga tatcggtatt accttgcagt
     3781 atgaagagtt gattgctgct tatgaaaaac aacgaccagc ctactggcag gattagaaaa
     3841 aatagaaaag gaaatatagt aaactgaaat aagatgtaaa caaatgaatt ggagcttaac
     3901 atccatttcc agcaattttt tagaaactac agtggactat tctggattca acacattata
     3961 aaattatgac aaaacacatt cacaagaagg ctacgacatt ttaaaaggtg agggcggatg
     4021 tatcgtttgc cctactaaag ttggttacat tatcatgacc agcaacaagg caggacttga
     4081 gcgtaagttc gcagccaaag aacgtaagcg taacaaacca ggtgttgttc tctgcggtag
     4141 catggatgaa cttcgcgctt tagcgcaact caacccagaa attgaagcat tctactaaaa
     4201 acattggatg aagatattct tcttggttgt atccttcctt ggaaaccaga agcctttgaa
     4261 aaactcaaag catacgggga tggccgtgaa gaacttatga ctgatgtacg tggtactagc
     4321 tgttttgtta tcaagtttgg aaaagcaggt gaacaattgg ctgccaagct ttgggaagaa
     4381 ggtaaaatgg tctacgcctc atatgcttca atgacaaaac gattgaaact cgctatgaga
     4441 gcaaggtgta atggtgtcta tggtcgataa ggacggcaaa ctcatcccag aacaaggagg
     4501 agcacgttca acttcaccag caccagttgt gatccgtaaa gggcttgaca ttgataaaat
     4561 catgatgcac ctgtcagata cttttaactc atgggactac cgtcaggttg agtattatta
     4621 agataaaaaa agaagtctag tgttatgaga tattaaagct tctaacactg gactttttgt
     4681 ttagaatttc ttttcttttt ctataggata tggtattcta tgtagaaaat atatgttaat
     4741 aagtaatgcc aatatttaaa catcattagt aaaaggag
//
LOCUS       DAVAAL010000128         4766 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVAAL010000128 DAVAAL010000000
VERSION     DAVAAL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.130"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            257..1636
                     /locus_tag="VQM60_001782"
     CDS             257..1636
                     /locus_tag="VQM60_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET1081612.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            1650..2315
                     /locus_tag="VQM60_001783"
     CDS             1650..2315
                     /locus_tag="VQM60_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET1081613.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            2670..3209
                     /locus_tag="VQM60_001784"
     CDS             2670..3209
                     /locus_tag="VQM60_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET1081614.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQKNKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTALLRASQF"
     gene            3276..3686
                     /locus_tag="VQM60_001785"
     CDS             3276..3686
                     /locus_tag="VQM60_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET1081615.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQENPKVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            3802..>4766
                     /gene="pavB"
                     /locus_tag="VQM60_001786"
     CDS             3802..>4766
                     /gene="pavB"
                     /locus_tag="VQM60_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET1081616.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQENPELTDALKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLE"
BASE COUNT         1344 a          900 c         1071 g         1451 t
ORIGIN      
        1 acctgtgact agctttctaa tcgatgcctt ggttttcatt gcctataatc aaaaagagaa
       61 attttctcct gaaaagcata tagagtagct ggcgttaaaa gctcctgtct tgcttttttg
      121 acctatagtc atatctatca agtattgttc ttgcctaagc tatcaataaa aaggtggcat
      181 tttttaggct tggtgttagt gaattttgcc ttatcctatc taagtcattt cgagcttttt
      241 atggtacaat ggaaacatgt tattcaaatt atctaaggaa aaaatagagc taggcttatc
      301 tcgtttatcg ccagcccgtc gtattttttt gagttttgcc ttggtcattt tactaggctc
      361 tcttcttttg agcttgccct ttgtccaagt tgaaagctca cgagcgactt attttgatca
      421 tcttttcact gctgtctctg cagtctgtgt gacaggtctc tcaacccttc cagtagctca
      481 cacctataat atctggggcc aaataatctg tttgctcttg attcagatcg gtggtctagg
      541 gctcatgacc tttattgggg ttttctatat ccagagcaag caaaagctta gtcttcgtag
      601 ccgtgcaact attcaggata gttttagtta tggagaaact cgatctttga gaaagtttgt
      661 ctattctatt tttctcacga cctttttggt tgagagcttg ggagctattt tgcttagttt
      721 tcgccttatt cctcaacttg gctggggacg tggtcttttt agttccattt ttctagcgat
      781 ctcagccttc tgtaatgccg gttttgataa tttagggagc accagtttat ttgcttttca
      841 gaccgattta ctggtcaatc tggtgattgc aggcttgatt attacaggcg gccttggttt
      901 tatggtctgg tttgatttgg ctggtcatgt aggaagaaag aaaaaaggac gtctgcactt
      961 tcatacgaag cttgtattat tattgactat aggtttgttg ttatttggaa cggcaactac
     1021 tctctttctt gagtggaaca atgctggaac gattggcaat ctccctgttg ccgataaggt
     1081 tttagttagc ttttttcaaa cagtgacgat gcgaacagct ggcttttcta cgatagatta
     1141 tactcaggct catcctgtga ctcttttgat ttatatctta cagatgtttc taggtggggc
     1201 acctggagga acagctgggg gactcaagat tacgacattt tttgtcctct tggtctttgc
     1261 acgaagtgag cttctaggct tgcctcatgc caatgttgcg agacgaacga tcgcgccgcg
     1321 aacggttcaa aaatccttta gtgtctttat tatctttttg atgagcttct tgataggatt
     1381 gattctgcta gggataacag ccaaaggcaa tcctcccttt atccacctca tatttgaaac
     1441 catttcagct cttagtacag ttggtgtaac ggcaaatctg actcctgacc ttgggaaatt
     1501 ggctctcagt gttatcatgc cacttatgtt tatgggacga attggtccct tgaccttgtt
     1561 tgttagcttg gcagattacc atccagaaaa gaaagatatg attcactata tgaaagcaga
     1621 tattagtatt ggttaagaaa ggaaagagca tgtcagatcg tacgattgga attttgggct
     1681 tgggaatttt tgggagcagt gtcctagctg ccctagccaa gcaggatatg aatattatcg
     1741 ctattgatga ccacgcagag cgcatcaatc agtttgagcc agttttggcg cgtggagtga
     1801 ttggtgacat cacagatgaa gaattattga gatcagcagg gattgatacc tgcgataccg
     1861 ttgtagtcgc gacaggtgaa aatctggagt cgagtgtgct tgcggttatg cactgtaaga
     1921 gtttgggggt accgactgtt attgctaagg tcaaaagtca gaccgctaag aaagtgctag
     1981 aaaagattgg agctgactcg gttatctcgc cagagtatga aatggggcag tctctagcac
     2041 agaccattct tttccataat agtgttgatg tctttcagtt ggataaaaat gtgtctatcg
     2101 tggagatgaa aattcctcag tcttgggcag gtcaaagtct gagtaaatta gacctccgtg
     2161 gcaaatacaa tctgaatatt ttgggtttcc gagagcagga aaattcccca ttggatgttg
     2221 aatttggacc agatgacctc ttgaaagcag atacctatat tttggcagtc atcaacaacc
     2281 agtatttgga taccctagta gcattgaatt cgtaaagagg gatgacccct cttttttgat
     2341 gcccaagatg gcaaatagag acagaagccc cttgtcttct agtaaaagtt cttcaaagac
     2401 tggactttat ggtaaaatag aaagaagtga caagagagag taatactcaa tgaaaatcaa
     2461 agatcaaact aggaaactag ctacgggctg ctcaaaacac tgttttgagg ttgtggatag
     2521 aactgacgaa gtcagctcaa aacactgttt tgaggttgca gatagaactg acgaagtcag
     2581 taacatctat acggcaaggc gacgttgacg cggtttgaag agattttcga agagtataag
     2641 aaaaaatcag tcccctaaag gagcagatta tgaagttatt gtctatcgcc atttctagct
     2701 ataatgcagc agcctatctt cattactgtg tggagtcgct agtgattggt ggtgagcaag
     2761 ttgggatttt gattatcaat gacgggtctc aggatcagac tcaggaaatc gctgagtgtt
     2821 tagctagcaa gtatcctaat atcgttagag ccatctatca gaaaaataaa tgccatggcg
     2881 gtgcggtcaa tcgtggcttg gcagaggctt ctgggcgcta ttttaaagta gttgacagtg
     2941 atgactgggt ggatcctcgt gcctacttga aaattcttga aaccttgcag gaacttgaga
     3001 gcaagggtca agaggtggat gtctttgtga ccaattttgt ctatgaaaag gaagggcagt
     3061 ctcgtaagaa gagtatgagt tacgattcag tcttgcctgt tcggcagatt tttggctggg
     3121 accaggtcgg aaatttctcc aaaggccagt ataccatgat gcactcgctg atttatcgga
     3181 cagctttgtt gcgtgctagc cagttctaat tgcctgaaca tactttttat gtcgataatc
     3241 tctttgtctt tacgcccctt cagcaggtca agaccatgta ctatctgcct gtcgatttct
     3301 atcgttattt gattgggcgt gaggaccagt ctgtcaatga gcaagtgatg attaagtgca
     3361 ttgaccagca actcaaggtc aatcgactct tggtagacca gcttgatttg tcccaagtga
     3421 gtcatcctaa aatgcgagaa tatctgctga atcatattga aatcacgacg gtgatttcca
     3481 gtaccctgct caaccgatct gaaacagcgg agcatctggc aaaaaaacgc caattgtgga
     3541 cctatattca gcaggaaaat ccaaaagtct ttcaggctat tcgtaagacc atgttgagcc
     3601 gtttgaccaa acattctgtc ttgccagatc gcaaactgtc caatgtcgtc tatcaaatca
     3661 ccaaatctgt ttatggattt aattaatata agtgttttat aagagggatt taagaaaaat
     3721 tttaactttt tcttagtcct ttttaatttc aggagattat actagagtca tcaaataaag
     3781 aaagactcta aggagaatcc tatgaaattc aatccaaatc aaagatatac tcgttggtct
     3841 attcgccgtc tcagtgtcgg tgttgcctca gttgttgtgg ctagtggctt ctttgtccta
     3901 gttggtcagc caagttctgt acgtgccgat gtggtcaatc cgaccccagg tcaagtctta
     3961 cctgaagaga catcgggaac gaaagagggt gacttatcag aaaaaccagg agacaccgtt
     4021 ctcactcaag cgaaacctga gggcgttact ggaaatacga attcacttcc gacacctaca
     4081 gaaagaactg aagtgagcga ggaaacaaac tcttctagtc tggatacact ttttgaaaaa
     4141 gatgaagaag ctcaagaaaa tccagagcta acagatgcct taaaagaaac tgtagataca
     4201 gctgatgtgg atgggacaca agcaagtcca gcagaaacta ctcctgaaca agtaaaaggt
     4261 ggagtgaaag aaaatacaaa agacagcatc gatgttcctg ctgcttatct tgaaaaagct
     4321 gaagggaaag gtcctttcac tgccggtgta aaccaagtaa ttccttatga actattcgct
     4381 ggtgatggta tgttaactcg tctattacta aaagcttcgg ataatgctcc ttggtctgac
     4441 aatggtactg ctaaaaatcc tgctttacct cctcttgaag gattaacaaa agggaaatac
     4501 ttctatgaag tagacttaaa tggcaatact gttggtaaac aaggtcaagc tttaattgat
     4561 caacttcgcg ctaatggtac tcaaacttat aaagctactg ttaaagttta cggaaataaa
     4621 gacggtaaag ctgacttgac taatctagtt gctactaaaa atgtagacat caacatcaat
     4681 ggattagttg ctaaagaaac tgtcgaaaaa gctgttaaag ataacgttaa agacagtatc
     4741 gatgttccag cagcctacct agaaaa
//
LOCUS       DAVAAL010000129         4726 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVAAL010000129 DAVAAL010000000
VERSION     DAVAAL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.131"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            503..1201
                     /locus_tag="VQM60_001787"
     CDS             503..1201
                     /locus_tag="VQM60_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET1081617.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKA"
     misc_feature    1266..1353
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1389..1703
                     /gene="rplU"
                     /locus_tag="VQM60_001788"
     CDS             1389..1703
                     /gene="rplU"
                     /locus_tag="VQM60_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET1081618.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1719..2063
                     /locus_tag="VQM60_001789"
     CDS             1719..2063
                     /locus_tag="VQM60_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET1081619.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            2080..2373
                     /gene="rpmA"
                     /locus_tag="VQM60_001790"
     CDS             2080..2373
                     /gene="rpmA"
                     /locus_tag="VQM60_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET1081620.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2578..2783)
                     /locus_tag="VQM60_001791"
                     /pseudo
     CDS             complement(2578..2783)
                     /locus_tag="VQM60_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            3221..4138
                     /locus_tag="VQM60_001792"
     CDS             3221..4138
                     /locus_tag="VQM60_001792"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET1081621.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            complement(4231..>4726)
                     /locus_tag="VQM60_001793"
     CDS             complement(4231..>4726)
                     /locus_tag="VQM60_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET1081622.1"
                     /translation="GLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCILML
                     ILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDLGR
                     GVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEGFT
                     FEKE"
BASE COUNT         1461 a          791 c          951 g         1523 t
ORIGIN      
        1 gatttgccaa caaattgcat agcagcaagt tcagcttggc tttgttccat ttgtttttga
       61 agtttttgtg cttgacgcat catgttttgc atgttcatca taagatttct aaagttcctt
      121 tcatctcaag gggataatcc ttgttgttaa caagtgcctg attttctctc ctaattgtta
      181 ttagtagcat agccatcaag cccaagtatt gattttatta taccattttt ttaagacata
      241 gtcttttatt ggtgagagtt atttatgtca aggagtgatt agaaataaat taaaataatg
      301 atttttgcta agaggggctc tattctagtt tcatggaaat gattttacta aagattcgta
      361 tgctttgttt gcaaacatgt tacctctatc tatttttgga taaatagttg tctattatta
      421 tttttatgct aatagtaaaa tctaaatggt agtctacgat tttttagaaa gaaatggtac
      481 aatatttcta agagaaaata caatgggagg gaaaatgagg ttattaccta taagaaaaat
      541 atcacgtcag tctaaaaggt tagcactttt tttgacgttt tgtgctggat atgtggatgc
      601 ttacactttt attgttcgcg ggaataccct tgtagctgga caaactggaa atgttgtctt
      661 tctttcagta gaattaatta aaaataatgt ttcggatgtt agggacaagg ttctcacctt
      721 gctagcgttt atgatgggag tctttttatt aacgatttat aaggaaaaat tgagaattgt
      781 gaaaaaacct attctgtcct tgattccctt ggcaatctta tcaatcatta ttgcttttgt
      841 gccgcaaact gtggataata tctatctagt gccgcccttg gccttctgta tgggactggt
      901 gacaactgct tttggagaag tgtcgggtat tgcctataat aacgctttta tgacagggaa
      961 tatcaaacgg accatgctgg cttttggaga ttatttccga accaagcaca ctcctttttt
     1021 gcgtgaagga ttcatatttg ttagcctgct tagtagtttt gtccttggcg ttgtcttttc
     1081 agcctatttg acgattttct atcatgaaaa gaccattctt ggtgttccta ttatgatgag
     1141 cgttttttac ctcagcatgc tttttgcctc ttggcagaaa aaagtaaaag aaaaagcttg
     1201 attttaggtg ttattacttg taagaaaata ggagatatgc tatacttgaa gagttgacta
     1261 tgcacgatcc tgtgcaaccg cacgaacatc gttgcttgat tattcaagtt taagtggttc
     1321 gtcccacgat gctaggcgag tcttcacaaa aaatcgggga aacccataaa aatcatagga
     1381 ggtgcataat gagcacatac gcaattatca aaactggcgg aaaacaagtt aaagttgaag
     1441 ttggtcaagc agtttacgtt gaaaaattga acgttgaagc tggtcaagaa gttactttta
     1501 acgaagttgt tcttgttggt ggtgaaaata ctgttgtcgg aactccactt gttgctggag
     1561 ctactgtagt tggaactgtt gaaaaacaag gaaaacaaaa gaaagtggtt acttacaagt
     1621 acaaacctaa aaaaggtagc caccgtaaac aaggtcaccg tcaaccatat acaaaagttg
     1681 tcatcaacgc aatcaacgct taattttaag gagaacacat gatacaggca gtctttgaga
     1741 gagccgaaga tggcgagctg aggagtgcgg aaattactgg acacgccgag agtggcgaat
     1801 acggcttaga tgtcgtgtgt gcatcggttt ctacgcttgc cattaacttt atcaattcta
     1861 ttgagaaatt tgcaggctat gaaccaatcc tagaattaaa cgaagatgaa ggtggctatc
     1921 tgatggttga aataccaaaa gatcttcctt cacaccagag agaaatgacc cagttattct
     1981 ttgaatcatt tttcttaggt atggcaaact tatcggagaa ctattctgag ttcgtccaaa
     2041 ccagagttat cacagaaaac taacacggag gaaaacatta tgttaaaaat gactcttaac
     2101 aacttgcaac ttttcgccca caaaaaaggt ggaggttcta catcaaacgg acgtgattca
     2161 caagcaaaac gtcttggagc taaagcagct gacggacaaa ctgtaacagg tggatcaatc
     2221 ctttaccgtc aacgtggtac acacatctat ccaggtgtaa acgttggtcg tggtggagac
     2281 gatactttgt tcgctaaagt tgaaggcgta gtacgctttg aacgtaaagg acgcgataaa
     2341 aaacaagttt ctgtttaccc aatcgctaaa taaaaaggtc cattgaacct tttatcccga
     2401 accttgaaat gtaaaggtga ggaagctaga aacagcttaa aataaggctt tccgagtttt
     2461 cggagagcct gtttttttgt tttggtaccc atacttttta atatgtatag tggattgaat
     2521 ctggaataga acactgttaa aaattgatat gagtttccta ctcgatttct tcagatttta
     2581 tttcaattga ctataagaac tgctatctgg tttcaaatat tggcttgtcg tcccttcact
     2641 tgtctgaatg gtttgagccg ttccttctga taagggaaaa gatattcttt agctgaaact
     2701 gtctgagaat ccaagctaac agatggagag aaaaatagcc cagtctgaag cacactagat
     2761 gggagttgtt ggaaagcagt catctagttt ttctttgctg ttttggcttt tttctttgtc
     2821 atcagggaaa gtgacttgct tcaaatcagg gagtttagga aattcttcaa acatcttgcc
     2881 caagttgtct cattttgtaa attttacagc cataggtgta cctcgattct aaattctacc
     2941 ttttcggcac caattagtaa tttaaagttc tcaggagtta gttgcattgc tttttcatac
     3001 ataagcatag tatgacactt tatggttacc gaacaagtct aatcactcct tgcaaaattt
     3061 tgaagatgtc ctacaagttt ttgagaagaa tattgcaaag ttcatagtta agcaacgata
     3121 gaataaagtg ctttttgaaa acaggtacac ttcatgtgaa aaaaacaaca tttcagattt
     3181 tctgttccag aaatagctat cgctatgata taattatttt atgattatta ctattcctat
     3241 aaaaaaccaa aaagatattg gcacaccatc tgattcagtc gttgttctcg gctattttga
     3301 tggcatacat aaggggcatc aagaattatt tcgtgttgcc aacaaggctg cgagaaagga
     3361 tttattacct atcgtcgtta tgacctttaa tgaatctcca aagatcgctt tagagcctta
     3421 tcatccagat ttgtttttgc atattttgaa ccctgctgaa cgtgaaagaa aattaaagcg
     3481 cgaaggtgta gaagaattat atctccttga ttttagtagt caattcgcta gtctcactgc
     3541 acaagaattc tttgcaactt atatcaaggc tatgaatgcc aaaattattg ttgcaggttt
     3601 tgattataca tttggttctg acaaaaaaac agcagaagat ttaaaggatt actttgatgg
     3661 agaagttatc attgttccac ctgtagaaga tgagaaagga aagattagtt caactcgtat
     3721 ccgtcaagct attttagatg gaaatgtgaa agaagcagga aaacttttgg gggcaccgct
     3781 tccatcaaga ggtatggtgg ttcatggtaa tgctcgtggt cgtacaattg gttatccgac
     3841 agcgaattta gtgcttttag accgtactta tatgccagca gatggcgttt atgtcgttga
     3901 tgttgagatt caaagacaga agtatcgtgc tatggctagt gtcgggaaaa atgtgacctt
     3961 tgatggagaa gaagcacgtt ttgaagtcaa tatttttgat tttaatcaag atatttatgg
     4021 ggaaaccgtc atggtttatt ggcttgatcg cattcgtgat atgaccaaat ttgactcagt
     4081 tgaccaatta gtggatcagt taaaggctga tgaagaagta actcggaatt ggtcttaaga
     4141 gcttgagtaa ataaaataaa aaagaggttg tctgtaaccc aaaagataga tgatttagtc
     4201 taacttttga ggtcacgaca ttacctcttt ttattctttt tcaaaggtga agccttctcc
     4261 taggatttca tgggcttctg taatagttat aaaggcttga ggatcgattc gatgaatcat
     4321 ttccttcgtt ttcacaattt catttcttcc gacaatacag tagatgattt tcaaattttc
     4381 tttactatag tagccttgac cagaaataaa agtaacacct cttccgaggt catcattaat
     4441 cgccttagca agttggtcag gacgttttgt gataatcata aagcctttgc cggcatatcc
     4501 tccttcacca atcaaatcaa taacacgaga aacaataaaa tcaaacaaaa gcgtgtagga
     4561 aaccaatctc aaatccttga agattaggag aatcaacatg agaatacaaa aatctaagat
     4621 aaagagcagt tttcctatgg atatatgagt gtatttgttg agaatacgag ctagaatatc
     4681 agttccgcca gttgtacctc cagcattaaa aataattcca aggcca
//
LOCUS       DAVAAL010000130         4711 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVAAL010000130 DAVAAL010000000
VERSION     DAVAAL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.132"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(98..2356)
                     /locus_tag="VQM60_001794"
     CDS             complement(98..2356)
                     /locus_tag="VQM60_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET1081623.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2585..2815)
                     /locus_tag="VQM60_001795"
     CDS             complement(2585..2815)
                     /locus_tag="VQM60_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET1081624.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3005..3688
                     /locus_tag="VQM60_001796"
     CDS             3005..3688
                     /locus_tag="VQM60_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET1081625.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3688..4422
                     /locus_tag="VQM60_001797"
     CDS             3688..4422
                     /locus_tag="VQM60_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET1081626.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKVDAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4555..>4711
                     /locus_tag="VQM60_001798"
     CDS             4555..>4711
                     /locus_tag="VQM60_001798"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677140.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET1081627.1"
                     /translation="MKEKRSDMTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVV
                     ILVGDNPA"
BASE COUNT         1307 a          887 c         1031 g         1486 t
ORIGIN      
        1 atcataatgg acaaagggca agctgtcaag aaattacagt tattttagta aaaaaagaag
       61 cagaaaaatt tcattttcta ctccttttct ataggattta tttttctttt tcagaacttt
      121 taacttcagc ttttttggca gatttaatct gaatgtttcc cttgctagtc ataactgctt
      181 tgagatcttt ttcgcttgga ttttcaaggt agtagtcagt gattgtgtcc tcaatatagt
      241 cttgaatagt ccgacgaagt gggcgtgctc ccatttttgg atcataacct aggtcaacca
      301 acttttcctt gaccttatca gttacatcca aacgaatgtt gttgctagag aggcgcttgt
      361 taacatctgc tagcatgagc tcgacaatct gaaggaggtt atccttgctg agagccttaa
      421 attcgataat gccatcaaaa cggttcataa actctgggct aaagaagtta ccgagttcac
      481 cgaggacaga attggtacgt ccttctctag cagcaccaaa tccaacgcta gcttcggtct
      541 ttcctgtacc tgcatttgag gtcatgataa tgatggcatc cttgaagcta acggtgcgtc
      601 cttgcccgtc tgtcaaacga ccatcgtcca agacttgaag aaacatgtgc ataacatctg
      661 ggtgagcttt ttccacttca tcgagaagga tgagagaata tggattgtgg cgaacttttt
      721 cagttaattg accagcctca tcatagccaa cataacctgg aggggcgccg accaacttag
      781 ccacactatg tttttccatg tattcactca tatcaaagcg aatcatacta tcagcagaac
      841 caaaaagttc gatagccagt tgtttggaaa gttctgtctt accgacacca gttggcccaa
      901 cgaagaggaa gcttccgatt gggcggttag gggtaccaag tccgacacga ttacggcgaa
      961 tagccttggc aatcttatcg actgcatcat cttggccaat aacatgagac ttgagatctt
     1021 cggctagatg gatgagttga gattgttctt tctctttcaa atcaccaaca gggatattgg
     1081 ttttctgctc gataatgtgc tcaatagttt tctcgctgat gataggagta tcctggtctg
     1141 tgatcttttt cttttgcatt tccttatact tggcaatctg gtcgcggaag taggccgcct
     1201 tctcaaaatc ttcttctcgt gtagcttgag acttgagatt ttcagcctca atcaagcgct
     1261 gatcaattac tttaggatcc acaaaattca aggtcaagtt catcttagaa ccagcttcat
     1321 ctaggaggtc aatggccttg tcaggcaaga agcgatcttg gatgtagcga ttggaaagag
     1381 ttgcagctgc ttcaatcgca gcatctgtat attgaacgtg gtggtaatct tcgtatttct
     1441 tttgaatccc tttgagaata gtgattgttt cgtccaccgt tggttcatcg actttaacag
     1501 gctgcatacg acgctcgagg gcagcatcct tttcaatgat acggtattca ttgagggtag
     1561 tagcaccgac tagttgcagt tctccacgag caagggctgg cttgaggata tttcctgcgt
     1621 ccatattacc atcactcgca gaaccagcac caacaatttc atggatttca tcgataaaga
     1681 ggatgatgtc ttcacgtttg cgaatttctt ccatgagttt ttgcatgcgt tcttcaaatt
     1741 gtcctcgaat ccccgttcct tgaactaagc taaccacatc cagacggatg acttgtttac
     1801 cttggagttt atgtggcaca tcgccatcga caattttctg agctagacct tcgacaacgg
     1861 ccgtttttcc tacaccaggt tcaccgataa ggacaggatt attcttggtt ctacgattga
     1921 gaatctcgat gacacggata atctcatcgt cgcgcccaat aacggggtca atgtctccac
     1981 gacgggcaat ttcagttaca ttaataccaa attcttccag caggcctttt tcttggctag
     2041 gtggcggagt ttgagcagat ccacgatttt gggaaccata accgccgttt ccaccgtaac
     2101 ctccacctga ttgggttggg ggaataggcg gagtattgct agaaggtctg aaattgttta
     2161 gatcattgaa gaaatcacca aagggatcga agtcacgatt gttcagatcc gtcatacctt
     2221 tgaagaggct attgttagga tctgttttga taatcttata gcagttttga cagaggtcaa
     2281 tttgtttttg ttttccattg agattggtgt aaagatgaat tgttgagtcg ttaattttac
     2341 agttttgaca aagcatacat ctacctcatt tctttcttta gcctgacttg tttaaaggtc
     2401 aaaaatagtc aaattctata ctctcattat aacaggattg gggtgtaaag caagtaaaaa
     2461 gattgaagct ttgagaagtt gttacaatga aaatttttgt gaatttggac tataaaaaaa
     2521 tcctatttgt gagacaaaaa ggattttact tagacatgca gggacaatcc caacttgttt
     2581 tagattagta gagaagttcg taaatttcca ttgcgattaa gtcaatgcta tcaaacgtat
     2641 acgtttcgcg atcttctacg tcacgaaggg taaagattgg gccgttttct tcgtcgtggc
     2701 tgtacgcaac ttctgcaaca acaactcctt cgcgttcaaa attacgtttt aaatttccgc
     2761 catcgtttgc cattgcttca aggcggttga tgattctaac caaatgtgat tccattttga
     2821 ttctccttca tttcttatcg ttactatttt accataaagg agggcaactt gactagaaaa
     2881 aactaagatt tctcgctaat tctgctagtt taaagatttt taaataaatt ggataaaaaa
     2941 tttaaatttt aattcaaaac atgttataat catacagtat tctattttag aaagcagtgt
     3001 gactatgaat ttttcttttt tacctaagta tttaccttat tttaactatg gggctgttgt
     3061 gacgattctt atttctatct gtgttatctt tttgggaact attttgggtg ttgtcttggc
     3121 ttttgggcaa cgttcaaagt ttaaaccgct tgtttggttg gccaacttgt acgtttggat
     3181 tttccgtggg acaccgatga tggttcaaat tatgattgcc tttgctctta tgcatatcaa
     3241 tgctccgact attcagattg gaattttagg tgttgatttt tcgcgtctga ttccagggat
     3301 tttgattatc tctatgaata gtggtgctta tgtttcggag actgttcgtg ccggaatcaa
     3361 tgcggttcca aaaggtcagc tagaggcggc ttattcgcta gggattcgtc ttaaaaatgc
     3421 gatgcgttat gtgattttgc cacaagcagt caaaaatatc ttgccagcat tggggaacga
     3481 atttatcacc attatcaagg acagctccct cttatcagct attggggtca tggagttgtg
     3541 gaatggggct acaacagttt ctacaacaac ctatctacct ttaacaccac ttttatttgc
     3601 agcattttac tacttgatta tgacctctat tctgacagta gccttgaaag cttttgaaaa
     3661 acatatggga caaggagata agaaataatg acagaaacct tgataaaaat tgaaaattta
     3721 cataaatctt ttggaaagaa tgaagtattg aagggcatca acctcgagat taaaagagga
     3781 gaagttgtcg ttatcatcgg tccttcaggg agtgggaaat ctaccttgct tcgctctatg
     3841 aatttgttgg aggaagcaac caaggggaag cttatctttg agggagtcga tattacggac
     3901 aagaagaatg acctgtttgc catgcgtgag aagatgggca tggttttcca acaatttaac
     3961 ctctttccta atatgactgt gatggaaaat attaccttgt cacctatcaa gactaaaggt
     4021 gaaagcaggg aagttgctga gaagagagcc caagagcttt tggaaaaagt tggtttgcca
     4081 gataaggtag acgcttatcc acagagtctg tcgggtgggc agcaacaacg gattgccatc
     4141 gcgcgtgggt tggctatgga accagatgtt ttgctctttg acgaaccaac ttcagccttg
     4201 gatcctgaaa tggtaggtga ggtattggct gttatgcaag accttgccaa gtcagggatg
     4261 actatggtta tcgtaacaca tgagatggga tttgcccgtg aggtggcaga tcgtgttatc
     4321 tttatggcag acggtgtggt tgttgaagat gggacacctg agcagatttt tgaacaaacc
     4381 caagaacaac ggactaaaga tttcttgagt aaggttttat aatctatttt aaaatttcaa
     4441 gacaagatta gtgaaaagtt caaccagagc tttttcttat agtttgaaac tataggataa
     4501 cctaggaaag aagtgttaga gggcactagc agtttttggt ataatggaag atatttgaaa
     4561 gaaaagagaa gtgatatgac acagattatt gatgggaaag ctttagcggc caaattgcag
     4621 gggcagttgg ctgaaaagac tgcaaaatta aaggaagaaa cagctctagt gcctggtttg
     4681 gtagtgattt tggttgggga taatccagcc a
//
LOCUS       DAVAAL010000131         4687 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVAAL010000131 DAVAAL010000000
VERSION     DAVAAL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.133"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..765
                     /locus_tag="VQM60_001799"
     CDS             <1..765
                     /locus_tag="VQM60_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET1081628.1"
                     /translation="LGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMDEPLSNL
                     DAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGTGTIGRV
                     EQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGALKVLRE
                     KGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQVGKDEFV
                     AKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            1266..2024
                     /locus_tag="VQM60_001800"
     CDS             1266..2024
                     /locus_tag="VQM60_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET1081629.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAIDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            2111..2623
                     /locus_tag="VQM60_001801"
     CDS             2111..2623
                     /locus_tag="VQM60_001801"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET1081630.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            2805..3749
                     /locus_tag="VQM60_001802"
     CDS             2805..3749
                     /locus_tag="VQM60_001802"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET1081631.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWINHAVYQETPFDWRKIEDDASAYGYL"
     gene            3758..4435
                     /locus_tag="VQM60_001803"
     CDS             3758..4435
                     /locus_tag="VQM60_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET1081632.1"
                     /translation="MTYLDAFKSGILVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            4534..>4687
                     /locus_tag="VQM60_001804"
     CDS             4534..>4687
                     /locus_tag="VQM60_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET1081633.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTV"
BASE COUNT         1454 a          790 c         1047 g         1396 t
ORIGIN      
        1 cttggattga aagaattctt ggaacgtaaa ccagctgacc tttcaggtgg tcaacgtcaa
       61 cgtgttgcca tggggcgtgc gattgtccgt gatgcgaaag tattcttgat ggacgaacct
      121 ttgtcaaact tggatgccaa acttcgtgta tcaatgcgtg ctgaaatcgc taaaattcac
      181 cgtcgtatcg gagctacaac tatctatgta actcacgacc aaacagaagc gatgacactt
      241 gcagaccgta tcgttattat gtcagctact aagaaccctg ctggtacagg tactatcgga
      301 cgtgtagaac aaatcggtac tcctcaagaa gtttacaaaa atccagttaa caaattcgtt
      361 gcaggattca tcggaagccc agctatgaac ttcatcaacg tgaaattggt tggtagcgaa
      421 attgtttctg acggtttccg tttgaaagtg ccagaaggag cattgaaagt tcttcgtgaa
      481 aaaggctacg aaggaaaaga attgatcttt ggtatccgtc cagaagacgt gaatgcagaa
      541 cctgctttcc ttgaaacatt cccagactgt gttgtaaaag cgactatctc tgtatcagaa
      601 ctgcttggtt cagaatctca cctttactgc caagttggta aagacgagtt tgttgcaaaa
      661 gttgatgctc gtgactactt gcaaacaggt gcaacagttg agcttggatt tgacttgaac
      721 aaagcacact tcttcgatgt agaaactgaa aaaacaatct actaaaataa ataaaattca
      781 aagcactaca agaaaagata tctctttatc aattgtagtg gagagatatc agttaatcta
      841 gggagagaaa caaaatgctt ctctcctttt tgctagggaa gtcatattat gcatctatat
      901 tgtgatgctc tttaatactc ttcgaaaatc tcttcaaacc acgtcaacgt cgccttgccg
      961 tacgtatgat tactgacttc gtcagtttta tctgcaacct caaagctgta ctttgagcag
     1021 cttacggcta gttttctagt tttctctttg atttccattg agtattattt gtgggtacca
     1081 tctacaagtg aagctatatg cgtaaactac gtgagcaatt gaattcgaac tagagaggta
     1141 ataataaatt tatgctatag ttatggtgac ttgtatgctt ttgattctag tttatcaaat
     1201 aatagattag aattgtcaga taatatcatt ttgtgttata atgaagaaaa aacagaggtg
     1261 ttcaaatgtc agaagcaggt cataagtttt tagcaaaatt ggggaaaaaa cgcttacgtc
     1321 caggtggaaa gcgtgccaca gattggttaa ttgcagaagg aggattttca aaagaaaaga
     1381 gaatactaga ggttgcgtgt aataggggaa ctacagcaat tgagttggca cagcgttttg
     1441 gttgcaagat aactgctatt gatatggatg ctcaagcttt agaagtggct aaaaaatctg
     1501 ctggaacggc aggtgttgct catttaatca gttttgaaag agcaaatgca atgaaacttc
     1561 cttatcaaga tgctagtttt gatattgtta taaatgaagc tatgctgact atgcaagccg
     1621 atcaagctaa gaaaaaatgt gtaatggaat atctaagggt attaaaacct ggaggtcttc
     1681 tcttgacaca tgatgtgctt cttaaggaag ctaaagagtc tatcagacag gaattatcac
     1741 aagcaattca tgtaaatgta ggtcctttaa ctcaagatgg ttgggaacag gtgatgatag
     1801 aatcaggtta ttgtgatgtg aaagcattga ctggtgaaat gacattaatg aaattatcgg
     1861 gtatgattta tgacgaaggt ttgctaggaa ctttgaaaat ttgtgtaaat gcttgtaaaa
     1921 aggagaatag aaagcagttt ttaactatgt ataaaatgtt tgctaagaat aaacagaaat
     1981 tgggctttat tgcgatggct agttataaat cgtcaaaacg ttagataatt attgaagtta
     2041 acttttcctt ttttctttct taaaaaatat gctataatag agagtaaaaa actttgaaag
     2101 aaagaaaaag atgaatttaa aagattacat tgcaacaatt gaaaattatc caaaggaagg
     2161 cattaccttc cgtgatatta gtcctttgat ggctgatgga aatgcttata gctacgctgt
     2221 tcgtgaaatc gttcagtatg ctactgacaa gaaagtcgac atgatcgtgg gacctgaagc
     2281 tcgtggattt atcgtgggtt gtccagttgc ctttgagttg ggaattggtt ttgcgcctgt
     2341 tcgtaagcca ggtaaattgc cacgcgaagt tatttctgct gactatgaaa aagagtacgg
     2401 tgtcgatacc ttgactatgc acgcggatgc cattaagcca ggtcaacgtg ttcttattgt
     2461 agatgacctt ttggcgacag gtggaactgt taaggcaact atcgagatga ttgaaaaact
     2521 tggtggtgtt atggcaggtt gtgccttcct tgttgaattg gatgaattga acggccgtga
     2581 aaaaattggt gactacgact acaaagttct tatgcattat taatgaaaac agtccctagg
     2641 gctgttttct ctacactagg atataaaaat agactataac tagttagaga aaaactataa
     2701 ttgaaaacta tatcttctta cagtataata aaaggactaa gtgtttgaga tttgtcttca
     2761 aacatatgca attattcctg aaagagtaca gttaggagag ggttatgccg attcgaattg
     2821 ataaaaaatt gccagctgtt gagattttac ggacagagaa tatctttgtc atggatgatc
     2881 aacgtgctgc ccaccaagat atccgtcctt tgaagatttt aattttaaat ctcatgccac
     2941 agaaaatggt cacagagacc cagttgttgc gccacttggc taatacaccc ctacaactgg
     3001 atattgattt tctctatatg gagagccacc gttctaaaac aactcgttca gagcacatgg
     3061 agaccttcta taaaactttt cctgaagtca aggatgagta ttttgatggg atgatcatca
     3121 cgggtgctcc agttgagcat ttaccatttg aggaagtgga ctattgggag gaatttagac
     3181 agatgcttga gtggtctaag actcatgtct attcgaccct tcatatctgt tggggggctc
     3241 aggctgggct ttatctgcgc tatggtgtag aaaaatacca gatggacagt aagctatcag
     3301 gtatttatcc tcaggacacc ctaaaagagg gtcaccttct atttagaggc tttgatgata
     3361 gctatgtatc ccctcattca cggcacacgg agatttctaa ggaagaggtc ttaaacaaga
     3421 ccaatctcga gattttatca gaaggacctc aggttggggt ttctattttg gccagtcgtg
     3481 atttacgaga aatttatagt tttggtcatt tggagtatga ccgtgatacc ttggcaaaag
     3541 agtattttcg agatcgtgat gcaggtttcg acccacatat tccagaaaat tactttaaag
     3601 atgatgatgt taatcaggta ccttgtcttt gttggtcttc atctgcagcc ctctttttca
     3661 gtaattggat aaaccatgcg gtctatcagg agacgccttt tgattggaga aagatagaag
     3721 atgatgcatc tgcatatggg tatttataag aggaattatg acatatttag acgcttttaa
     3781 atcaggtatc ttggttttac cgagtgccct gctcttgcat tttaaggaac tctttccttc
     3841 tagcgacgat tttctggttt ggcaattttt ctatttgcaa aatacgacag gcttagaaga
     3901 aatgtcgcca agccagattg ctgaaaggat tggcaaggaa atttcggatg tcaaccagtc
     3961 catttctaat ctgacggaaa ggggactgct ccagtatcgt actatcgaat taaatggcga
     4021 aattgaattg ctctttgatg ctagtttggc cttggaacgt ttggatgacc tgtttggagc
     4081 agttcattca agttcagacc agctgacacc tcaaaaccag ctcaaggatt tggtggaaac
     4141 cttccagcag gagttgggac gattgttgac gccttttgag attgaggatt tgaccaagac
     4201 actaaaggaa gatggaacca gtgctgactt gattaaggag gctcttcgtg aagctgtttt
     4261 gaatggaaaa ccaaactgga agtacattca ggcgattttg agaaactggc gccatgaagg
     4321 aatcaagagt gtggctcaaa ttgaggccaa gagagcagaa agagaagcaa gcaatcctca
     4381 gttgacacag gtatctgcag atttcagaaa tgccatggat ctctggaagg attaatccat
     4441 gcaagcaggc ttgaaatccg agtaagattt gcaagctgtg tataattgtg atagaataaa
     4501 atagaaaata aattgaaaaa agaggtatgt gaaatgtcac gtaaaccatt tatcgctggt
     4561 aactggaaaa tgaacaaaaa tccagaagaa gctaaagcat tcgttgaagc agttgcatca
     4621 aaacttcctt catcagatct tgttgaagca ggtatcgctg ctccagctct tgatttgaca
     4681 actgttc
//
LOCUS       DAVAAL010000132         4656 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVAAL010000132 DAVAAL010000000
VERSION     DAVAAL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.134"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(1..198)
                     /locus_tag="VQM60_001805"
                     /pseudo
     CDS             complement(1..198)
                     /locus_tag="VQM60_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            441..1615
                     /locus_tag="VQM60_001806"
                     /pseudo
     CDS             441..1615
                     /locus_tag="VQM60_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            1608..2483
                     /locus_tag="VQM60_001807"
     CDS             1608..2483
                     /locus_tag="VQM60_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET1081634.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIID
                     DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDE
                     YMPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIA
                     GTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENG
                     ITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKV
                     IKKAREEKIFNEFLF"
     gene            2570..4186
                     /locus_tag="VQM60_001808"
     CDS             2570..4186
                     /locus_tag="VQM60_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081635.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTSQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
BASE COUNT         1674 a          505 c          869 g         1608 t
ORIGIN      
        1 attcaattct ttaaggaacc gatttacttt ttctgggtct tgttcgcagt aggtacggct
       61 cttttttaga ctatatccca tagcctttag agcgtaatga atagttgttg gaggacagtt
      121 gaattcagca gctatttcag tcaaataagc atcgggatga gtttcaatat agatctttaa
      181 tttatctcta tcaacttttt caattaatag tggaagtttt cttaaagtta gtgtgttgaa
      241 acgtttttat acagtgctaa aatttgaagt gtagtgcaga tgtatttgta atagtattat
      301 agcaattctt tggagaaggt cgagacaaga gaaaataggt atctttgaga tataagcatg
      361 atttactata aaattattga gttattcata aaaatttctc tatcaaatat cataatgcgt
      421 cgtttaagga gatggttaca atgcaatata gttgtggaaa aataaatatt aatatccctg
      481 atggatatgg agacattaag gatatagtgt tttcggctca tattatagtt aggtataata
      541 atggacattg tggaggtata gatccgcata taattggact ttgtaaaaag cagatcagaa
      601 ggatgtctct atatcctatt ttgataatcg tatctagaga ttcaaaggtt attgatgatt
      661 ataaaaattt agatattgcc tatgttgatt gtactcaatg ttcaaataat tttgaaactg
      721 ccttacacgt taaaaatatt ttaaaattat taaaaatccg acttatacac tgtcatggct
      781 actcaactaa ttatttttta tatatgttaa aaaaacttga caagaatgga tttggaaagg
      841 ttaagacagt gataacatgc catggatggg ttgaatataa cttaaaaaag aaattcttga
      901 cttattttga tttttggaca tattctatgg gagatgcttt tatttgtgta tctgaaacta
      961 tgaaaaaaag attggagagt ataataaaaa aataagaaga tagttgcaat aaataacgga
     1021 attaatgtat caaatagcga tttagatgta gtaggtgttc aagactttaa aaaagaattt
     1081 tgtattccaa ataacaaaaa aatcatttgt tatgttgaaa ggttggatcc agaaaaaagg
     1141 ccggatagat tccttgaatt tgcagaaaaa ttatttttag tcagagaaga tgtgattttt
     1201 attatggctg gaaatggtag tatgtgggct gctttgaaag aaaaaatttg ccatttgaaa
     1261 tgtagagata atttcaggtt acttggtgaa atctatccag ctactatagt ttaccaaata
     1321 tcggatttat tgtatattcc ttctgataca gaaggaatac ctatgtgtgt tttagagtca
     1381 atgtcacaag gaactccggt tttagctagt aatgttggcg ggttaagtga aattattgaa
     1441 catagggttg atggattttt atttgagaag gaagatgttg agggagtgtg tgcttgtgct
     1501 aattttttac tcaatgattc tgagtatttg aaatatatag gtgagaatag taaatcaaaa
     1561 ataagaaaac atgagagtat atgatgaatt attagagaag agtagtcatg gataggaata
     1621 tagatcaaga attagtaagt attataatac caacacataa taggtatgaa tctctgatta
     1681 gagctgtgaa aagttgttta catcaaagtt ataaaaatat cgaagttata attattgatg
     1741 ataattatag taatgtaaat ttaagaaata aaattatcca tcaatttggc tataccaatc
     1801 atagaattaa gttaatttta agtaatgaag atttaggtgc aactaatgcc agaaacatag
     1861 gtatcaaaaa ttctagaggt aagtatatat catttttaga cgatgatgat gaatatatgc
     1921 cagatcgaat tttaaagttg atggcttgtt ttaaaaagag tagaatgaag aatttagctt
     1981 tagtttatag ttatggaata ataatttatc caaatggtac acgagaagag gagaagaccg
     2041 attttgttgg aaatcccttg tttgttcaaa tggttcacaa tatagcaggt acgtcatttt
     2101 ggttgtgtaa aaaagaggtg ctagaattaa ttaatggttt tgagaaaata gattcacatc
     2161 aggacggtgt tgttttatta aaactacttg ctcaaggata ccaaattgat atagtgcgag
     2221 aattcttggt gaattactac gctcacagta aagaaaacgg tatcactgga gtgacacaaa
     2281 aaacaattaa tgcagatgaa gaatattata attactgtag gaaatatttt aatttattga
     2341 gtttcaacga gagaatattg gttacaaaga aatattattc tttaaacata aagcggttac
     2401 tattaatagg agacaaatgc aaggctttaa aagtaatcaa gaaggcaaga gaagaaaaaa
     2461 tttttaacga atttcttttt tgaaatatat gttattatat cattttggtt ttttctattg
     2521 tatatatgac aactatgttc aattaaaatt tagaaagtga gaaactattg tgtatactat
     2581 tataaaatca aatataaaaa aatttagttt attaacgata tttattgttg ctggtcaatt
     2641 attgctaatt tatgcagcaa ctattaatgc tctggtgttg aatgaattaa ttgcgatgaa
     2701 tttagagcgg tttttgaaat tgtcaatcta ccaaatgatt gtctggtgtg ggataatatt
     2761 ccttgactgg gtagtgaaaa attatcaggt tgaagtgatc caagagttta atctagagat
     2821 tcgaaataga gttgccacag acatctctaa ctctacctat caagaatttc atagtaaatc
     2881 atcaggaaca tatctttcgt ggctaaataa tgatgttcag actttaaatg atcaggcttt
     2941 taaacaactt tttttagtaa taaaaggaat ttctggtact atatttgcag ttgtgactct
     3001 taatcactat cattggtcat tgactgtagc caccttgttt tcattaatga ttatgctact
     3061 tgtaccaaaa atctttgcat cgaaaatgcg agaagttagt ctaaatttaa ctagccaaaa
     3121 tgaagctttt ttaaaatcta gtgagactat attgaatgga tttgatgtgt tagcgtcctt
     3181 gaatctttta tatgtattgc ctaagaaaat taaagaagca ggaattttat taaagatggt
     3241 tatacaaaga aagacaactg tagaaacgtt agcaggcgct attagcttct ttctcaatat
     3301 tttttttcag atatctctcg tttttttaac aggctatctt gcaataaaag gaatagtgaa
     3361 aattggtact attgaagcaa taggagcact aacaggtgtt atttttacag cgctaggtga
     3421 attaggaggt caattatcct ctattattgg tacgaagcct atttttttaa aattgtattc
     3481 aattaatcca attgagtcaa ataaaatgaa tgatatcgaa ccaaatgagg tgaatagaga
     3541 ttttccgtta tatgaagcaa aaaatatttg ctataagtat ggagataaag aaatattaaa
     3601 aaacttaaat ttttgttttc aacgtaatga aaagtattta attttaggtg aaagtggaag
     3661 cgggaaatct acattattaa aattattgaa tggctttttg agagattata gtggagaatt
     3721 gcgattctgt ggggatgata taaaaaaaac ctcctattta aatatggttt cgaatgttct
     3781 atatgtagat caaaaagctt atttgtttga aggtacgatt agagataata ttttattgga
     3841 agaaaattat actgatgaag aaatactaca gtctttagaa caagttggtt tgagtgtaaa
     3901 agattttcct aataacattt tagattatta tgttggtgat gatgggagat tactgtcagg
     3961 agggcagaaa caaaaaatta ctttagctag aggcctaatt agaaataaga aaatagtatt
     4021 aattgacgag ggaacttctg ctatcgatag gagaacttcg ttagcgattg aacgtaagat
     4081 attagataga gaggatttga ctgtcattat tgttacccat gctccgcatc cggaacttaa
     4141 acaatatttt actaagatat atcaatttcc aaaggatttt atttaagaat ataggggagg
     4201 gaaacatgag attctattgt gaagcttatg aggtaattac tgaagagaaa aaagtatact
     4261 ttaatgatat tgagttagaa gttaagtatt cctctgtcga agaattgttt ataatatgtg
     4321 agaaattatt agataagaaa aaagtatcat tttttatgta gatgagaaac ccttaaggta
     4381 tctgttattt gactatatat ttctactagt tctagctaaa aaaatatccc aatcttatga
     4441 actgcacccc aaaagttaga tttattctgt ctaacttttg gggtgcagtt cattttgcat
     4501 agttttggca ttcatcctag atatccatat aaaatgtctg agattgttga aatgtctaaa
     4561 aaaatatcag atctaaacga taaaaaagaa tttcacaaaa agaactgtga gttgaggttt
     4621 caagatagtg ttcttgataa cataaatagt gtcctg
//
LOCUS       DAVAAL010000133         4621 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVAAL010000133 DAVAAL010000000
VERSION     DAVAAL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.135"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(60..1163)
                     /locus_tag="VQM60_001809"
     CDS             complement(60..1163)
                     /locus_tag="VQM60_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET1081636.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELILDPLNYPIENYVKRVKSE"
     gene            complement(1180..1977)
                     /locus_tag="VQM60_001810"
     CDS             complement(1180..1977)
                     /locus_tag="VQM60_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET1081637.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            complement(1964..2641)
                     /locus_tag="VQM60_001811"
     CDS             complement(1964..2641)
                     /locus_tag="VQM60_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET1081638.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            3190..3366
                     /locus_tag="VQM60_001812"
     CDS             3190..3366
                     /locus_tag="VQM60_001812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081639.1"
                     /translation="MDLLEKECLKCDKNFQQDDIWNYYHLSDKMPAQGWKIHISSQIK
                     DAVDILRLCINYPN"
     gene            3567..3809
                     /locus_tag="VQM60_001813"
     CDS             3567..3809
                     /locus_tag="VQM60_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081640.1"
                     /translation="MHSPVHYRYGAFLKKQAYDEKNKKVIYLLLDEKRKNYVEDKRQN
                     FPSLPSWKMDLFSEEEKRIYFQTTCEVSSKDSAINK"
     gene            3920..4255
                     /locus_tag="VQM60_001814"
     CDS             3920..4255
                     /locus_tag="VQM60_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HET1081641.1"
                     /translation="MNYDAEGEWTALDDIKNEAYMLKKLADKSYTTNLTDEFYIVDDY
                     FLVQEQVDGLNFEEFIRETEYSLNIREKSLDNIVNIVNDIHKLGYKIVDIAPTNFIYT
                     KKMYCPSNS"
     gene            complement(4265..>4621)
                     /locus_tag="VQM60_001815"
     CDS             complement(4265..>4621)
                     /locus_tag="VQM60_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET1081642.1"
                     /translation="EQVKSMLEQAFTEKYYENTILHSDQGWQYRHDSYHRFLESKGVQ
                     ASMSRKGNSQDNGMMESFFGILKSEMFYGYEKTFKSLNQLEQAIVDYIDYYNNKRIKV
                     KLKGLSPVQYRTKSFG"
BASE COUNT         1442 a          971 c          834 g         1374 t
ORIGIN      
        1 cagtgctttg agtaacccgc ggctagttac ctagtttgtt ctttgatttt cattgagtat
       61 tattcgcttt ttactcgttt gacatagttt tcaattgggt aatttagagg gtccaagatc
      121 aactccttgt cttggatcag ttgggctaga tggtaaccaa tgataggacc agttgtgagg
      181 cctgatgaac ctagtccact ggctgcatag acacctgcca attctggcac ctgcccaaag
      241 aagggagaga aatcactggt ataggcacgg attcccacac gctcagattt tgaagtagct
      301 tcagccaaaa tcagatagtg agtcaaggtg gcctcctcca tttgttggag caaggtttca
      361 tctaccgtca aatcaaatcc catgtcattt tcgtgggtag cgcctaagga caatttccca
      421 cctgcaaaag gaatcaaatc ccactcccct tctggcatga caacagggta atcttccatg
      481 tcttgggcaa gctgataatc ccgtagttgt cctttttgag gacggacatc cacttcataa
      541 cctaaaggct ctaacatgtc ccccaaccaa gctcccgttg ccaaaataac ctgatcaaac
      601 acctcttcgc caatctggta gcctgatgat aagagtgtca gagtcacttt ttctttgacc
      661 agcttgacat gactggcttc tagcagacga gttaccaaga gttggccatc tactctcgct
      721 ccaccagaag catagagcag gcggtcaaat ccctgcaaac cagggaataa ttcattagct
      781 gaagcttggt ccagaatggc taattgccct atcaagggag attcttctct gcgctggagg
      841 gccagttgat aaagttcttc caacttggat tcatcctttt tcaagagaaa gactcccgaa
      901 cgctggtaaa agtcgatttt ttgtcctgat ttctctaaat cagctaataa atccacataa
      961 aagtcagccc ccaagcgcgc catcttgtac caggctttat tacggcgttt ggaaaaccaa
     1021 ggactgataa ttcctgctgc ggccttggtg gcttgacctt gctcatggtc aaaaacggtc
     1081 acctctaggt cactttctct cgagaggtag taggcagctg ttgctcccac aattcctgct
     1141 ccaataatgg caactttttt cattgtcttc actttctaac tagatatgac ggaaaggatt
     1201 ggtcgatgct tgactaggta aaatctctag ttcccagcct tttttctctt tccattgagt
     1261 aaggagtgct gcgatttttt ccacaaaaag cacttcgata tagtgacctg gatccaatgc
     1321 taacaagcca tcagacagca tatcctgagc agtatggtag tagatatcac cagtgatata
     1381 gacatctgcc ccctttgcca aagcatcctt atagaaagac tgcccgcttc caccacaaat
     1441 tgctactctt gaaataggct tctgcaaatc attctcttga taatgcacca ttcgaaggct
     1501 atctaggtca aagacttgct tgacctgttg ggccaattcc caaaatgtct gaggctgaat
     1561 attcccaata cgtccaattc cacgttctgg acctgtttcc tgcagataag tcgtctcctc
     1621 gattcctagc atctgacaaa accagtcatt gagcccattt tcaacgatat caatattggt
     1681 atggctgaca taaactgcga tatcatgctt aatcaggtcg atgtaaatct gattttgcgg
     1741 acggctggca agcaagtcct tgataggacg aaagataggc gcgtgcttga cgataatcaa
     1801 gtccacaccc ttttcaatgg cctctgccac cgtctcttca cgaatatcga gggcaaccat
     1861 gaccctttgg atacccttgt ctaaagtgcc aatttgcaga ccacagctgt ctccctccat
     1921 agaaaattcc tgagggcaaa aggcttcata agcttggatc acttcacttg ctaacatgga
     1981 gcacctcctt gatagcttga atcttatcta ctagaacttg acgttcttcc agattttttt
     2041 ctgggatttg tccgagggcg aactctagct tctcagcttc tttttgccat ttttggacaa
     2101 atactggact gacttctttg gacaagaagg gaccaaagcg aacatcactg gctgatagct
     2161 tcatttgtcc tgcttccacc accaaaatct cataaaactt tccagcttct tctaagatgc
     2221 tttctgctac aatctggaat ccatgatcct gtagccagat acgcaagtcg tcttcacgat
     2281 tattgggctg gaggatcaaa cgctctacat tagctaactt ccccaaacct tcttctaaaa
     2341 tcctagcaat caaacgacca cccatgccag caatggtaat gacagacact tggtcagtct
     2401 cttcaaaagc tgccaagcca ttggctaaac ggacttggat tttctccttt aggccgtgag
     2461 cctcaacatt tttaaccgca gactgatagg gaccttccac cacctcacct gcaatagcgc
     2521 ttttgatttg gcctctctca accaactcga taggcagata agcatggtca cttcccacat
     2581 ctagtaaaat agccccctgt gacacaaagg aagctaccaa ttctaatctc tttgaaatca
     2641 tcttctctca ctttccaaaa ctctattacc tcttattata ccacatttca atcttcaact
     2701 tcccagtaat ataagcacct ctggcgaaag aagtttcaat gtcctaaagt aataagtgaa
     2761 tccaattgaa agattttaaa caatttgcaa aaatgtcaaa aaataaaaaa taaacagttt
     2821 attcagaaaa ttcttgacat ataaaaacac atggtagaat ataattagaa agttagaaaa
     2881 aataaaagtt tgactaaaat ttgtatttga aggtggtgtt cagataagaa atttagtcag
     2941 acgaaccacg aatttgctct atgctttctg gaatttatca taacaggagg atacagtcat
     3001 ggaacaaaca ttgtttgaat tagaactact tccagaggaa gatatcattg tcacaggtct
     3061 ccctaagtat tgttctttta cttgtttaat tacaggtcgc tagttatatt ttatataaaa
     3121 taagtagctt tacttacgga ataggctagt gctgtgtctc tagcctattt taataattag
     3181 gagtttgtta tggatttatt agagaaagaa tgtttaaaat gtgataaaaa tttccaacaa
     3241 gatgatattt ggaattacta tcatttgtca gataagatgc ctgcacaagg ctggaaaata
     3301 cacataagtt ctcaaatcaa agatgctgta gatattttaa gattgtgtat aaactatcca
     3361 aactaaataa ttgtagcttt aaagtcgtta aaaatttaga ggaattaaaa aaaattcatt
     3421 cccctaggga aatgaaccct actgctaaca aatttataac tctatatcct aagtcagaat
     3481 ctgaagccaa gagtatgatt tgtaatctta cgaataaact gtcagaattt aaggctccaa
     3541 aaatactatc tgactatcaa tgtggaatgc attctccagt tcattataga tatggggctt
     3601 ttttaaaaaa acaagcttat gatgaaaaaa ataaaaaagt catctattta ttgctagatg
     3661 aaaaaaggaa gaactatgta gaagataaga gacaaaattt ccctagtctt cctagctgga
     3721 aaatggattt attttcagaa gaagaaaaaa ggatttattt tcaaaccaca tgtgaagttt
     3781 caagtaagga ctctgcaata aataaataaa taaatacaaa atagaaaaaa ttattaagag
     3841 atcaaataaa ggaaatgttt atcgggcaat tagaaaatcg gacgagcaaa aagtaatcat
     3901 caagcagtca agaccttttg tgaattatga tgccgaagga gaatggacag cacttgatga
     3961 tataaaaaat gaggcttaca tgctaaaaaa attagccgat aaaagttata ctactaattt
     4021 aactgatgaa ttttatatcg tggatgatta ttttcttgtt caggaacaag ttgacgggtt
     4081 aaactttgaa gaatttattc gagaaacaga atactcctta aatattagag aaaaatcact
     4141 ggacaacatt gtgaatatag taaatgacat ccataaactg ggatataaaa ttgtagatat
     4201 agctcctaca aattttatat acaccaaaaa aatgtactgc ccctcaaaca gttagacaaa
     4261 taatttatcc aaaggattta gttctgtatt gcacagggct aagtcctttt agttttacct
     4321 taattcgttt attgttgtag taatcaatat agtctacaat agcttgttcc aattgattaa
     4381 gtgatttaaa tgttttctca tagccataaa acatttccga tttcagaatc ccaaagaagg
     4441 actccatcat accgttgtct tggctgttac ccttacgtga catggatgct tgaactccct
     4501 tactctctag gaaccgatga taagaatcgt gtcggtattg ccagccttgg tcactatgga
     4561 gaatcgtatt ctcatagtat ttctctgtaa aggcctgttc taacatactc ttcacttgtt
     4621 c
//
LOCUS       DAVAAL010000134         4598 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVAAL010000134 DAVAAL010000000
VERSION     DAVAAL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.136"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(133..1119)
                     /locus_tag="VQM60_001816"
     CDS             complement(133..1119)
                     /locus_tag="VQM60_001816"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET1081643.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            1313..3781
                     /gene="gyrA"
                     /locus_tag="VQM60_001817"
     CDS             1313..3781
                     /gene="gyrA"
                     /locus_tag="VQM60_001817"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET1081644.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            3781..4524
                     /gene="srtA"
                     /locus_tag="VQM60_001818"
     CDS             3781..4524
                     /gene="srtA"
                     /locus_tag="VQM60_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET1081645.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTAFNQPYKQFY"
BASE COUNT         1416 a          806 c         1025 g         1351 t
ORIGIN      
        1 aaataagata tgaacaaatc gattaggaat ttaaagcatt ttataacaac gttttagagt
       61 aacggggtac tatttcatat aatacaggaa aaaacaactc cctgatgatt caaggagttg
      121 tctatagtta aattagtttt tagaagcttc ttggaattct ggatttttcc atgcttcgtc
      181 aataatagct tgcaattctt tagcagatgc ttgcattttt tgagtttctg catcgttcaa
      241 tggaatgttt actggacgaa cgataccatg tgcaccaaca acagctggtt gaccgataaa
      301 gacattctca actccgtatt gaccttcttg gaatactgaa agtggaagta ctgcgttttc
      361 atcgtcaagg attgctttag tgatacgagc aagggctact gcgataccgt agtatgttgc
      421 accttttttg ttgatgattg tgtaggctgc atcacgaaca ccttcgaaca attcaatcaa
      481 ttcagcttct tgaacatttt gagtgtcttt aagaaattct tcaaggttta caccagcgat
      541 gttagcgtgt gaccaaacag cgaactcaga gtcaccgtgt tcacccatga tgtaggcgtg
      601 cactgaacga gcatccacat ccaatttttc agcaagtgct tgacggaaac gagctgagtc
      661 aagtgaagta cctgaaccga taacgcgttc tttagggaaa ccagagaatt tccaagttga
      721 gtaagtcaaa acgtcaactg ggttagcagc aacaaggaag atacctttga aaccagattc
      781 aacaacttga gttacgattg atttgttgat agcaaggttt ttacctacaa ggtcaagacg
      841 agtttcacct ggtttttgag gtgcacctgc agtgatcaca acaaggtcag cgtctgcaca
      901 gtcagagtat tgagctgcat agattttttt aggtgaagtg aaggcaaggg cgtgactaag
      961 gtcaagcgca tcaccaacag ctttttcatg caattgtgga atttcgataa ttccaagctc
     1021 ttgtgcaatt ccttggttaa caagtgcaaa agcgtaagat gaacctacag caccatcacc
     1081 gacaaggata acttttttgt gttgtttagt tgaagtcatt gttttaaaca tctccttaat
     1141 tttattaggg gattttccct agacaacttc attctatcac ttttaaaaaa ctttgtcacg
     1201 aatatgcctt atagttctcg atgtaaacgt tttagtggtt tagaggctga aatagatggg
     1261 aatttatggt ataatgttgt tacttactaa ttgtgaaatg aggcatttat taatgcagga
     1321 taaaaattta gtgaatgtca atctgacaaa ggagatgaag gcaagtttta tcgactacgc
     1381 catgagtgtt atcgtagcgc gagctcttcc tgatgttcga gatggcttaa aacctgttca
     1441 ccgtcgcatt ctctacggaa tgaatgaatt gggtgtgacc ccagacaaac cccataaaaa
     1501 atctgctcgt attacagggg atgtcatggg taaatatcac ccacacgggg attcctctat
     1561 ttatgaagcc atggtccgta tggctcaatg gtggagctac cgttacatgc ttgtagatgg
     1621 tcatgggaat tttggttcca tggatggaga tagtgctgcc gctcaacgtt ataccgaggc
     1681 acgtatgagc aagattgctc tggaaatgct tcgtgatatc aacaaaaata cagttgattt
     1741 cgttgataac tatgatgcca atgaacggga acccttggtc ttgccagcgc gttttccaaa
     1801 ccttttggtt aatggagcaa ctggtatcgc ggttgggatg gcaaccaata ttccacctca
     1861 taatctgggt gaaaccattg atgcagtgaa gttggtcatg gataatcctg aagtgactac
     1921 caaggacttg atggaagtct tgcctggacc agattttcca actggtgctc ttgtcatggg
     1981 gaaatcaggt atccataagg cttatgaaac aggtaaaggt tcgattgtcc tacgttctcg
     2041 tacagagatt gaaacgacta agactggtcg tgagcgtatc gttgtaacag aatttcctta
     2101 catggtcaat aaaaccaagg tgcatgagca tattgttcgc ttggttcagg aaaaacgcat
     2161 tgagggtatc acagcagtac gtgatgagtc aaaccgtgaa ggtgttcgat ttgttattga
     2221 agtcaagcgc gacgcctcag ccaatgttat tctcaataac ctcttcaaaa tgacccaaat
     2281 gcaaaccaat tttggtttca atatgctcgc tatccaaaat ggtataccga aaattttgtc
     2341 tcttcgtcag attttggatg cttatatcga gcaccaaaaa gaagtggttg ttcgtcgtac
     2401 acgttttgat aaggaaaaag cggaagcgcg cgctcatatc ttagaaggtc tcttgattgc
     2461 gctagaccat atcgacgaag tgattcgtat catccgtgct agtgaaacgg atgcggaagc
     2521 tcaagctgag ttgatgagca agtttaagct ttctgaacgt caaagtcaag ctatccttga
     2581 tatgcgtctt cgtcgtttga caggtttgga acgcgataag attcaatctg agtatgatga
     2641 cctcttggct ctgattgcgg atttagcaga tattcttgct aagcctgaac gtgtttctca
     2701 aattatcaaa gacgaattgg atgaagttaa acgtaaattt tctgataagc gccgtacaga
     2761 gttgatgatt ggacaggtct tgagtctcga ggatgaggac ttgattgaag aatcggatgt
     2821 cttgattacc ctttctaaca gaggctacat taagcgtttg gatcaggacg agttcactgc
     2881 tcaaaaacgt gggggtcgtg gtgtccaagg aacgggagtg aaagatgatg actttgttcg
     2941 tgagttagtg tcaactagca cccatgatca tctgctcttc ttcacaaaca agggacgtgt
     3001 ctatcgtctt aaaggttatg aaattcctga gtatggtcgg actgccaaag ggctaccagt
     3061 agtcaatctc ttgaaattgg atgaagacga aagtattcag acggttatca atgttgagtc
     3121 tgatcgcagt gatgatgctt atctcttctt tacaacccgt cacggtattg tgaagagaac
     3181 cagtgttaag gagtttgcca atattcgtca aaatggtctc aaagcgctga atttaaagga
     3241 tgaagatgag ttaatcaatg tcttgttggc agaaggagat atggatatta tcattggtac
     3301 caagtttggt tatgcagttc gctttaatca atcagccgtt cgtggtatga gtcgtatcgc
     3361 cactggtgtg aaaggtgtta accttcgtga aggagacaca gttgttggtg ccagcttgat
     3421 tactgatcaa gatgaggttc ttattatcac agaaaaagga tatggtaagc gtacagtcgc
     3481 tactgaatac ccaacaaaag gtcgtggtgg taagggaatg cagacagcta aaattaccga
     3541 aaaaaatggc ttgctggccg gtcttatgac tgttcaaggg gatgaggatt tgatgattat
     3601 cactgataca ggtgtcatga ttcgaaccaa tcttgccaat atttcacaaa caggacgtgc
     3661 aactatggga gttaaagtaa tgcgcctgga tcaagatgct cagatagtga ctttcacaac
     3721 ggttgcggtg gcagaaaaag aagaagttgg gacagaaaac gaaacagaag gtgaagcata
     3781 atgtctcaaa aaaataataa aaagaaaaac aagcgaaaaa atctgctgac aaatatccta
     3841 gcaggatttc tgatattact gtcactggct ttgattttta atactcaaat tcgaaatatt
     3901 ttcatagtct ggaataccaa taagtatcaa gttagccagg tatcaaaaga aaaattagaa
     3961 gaaaatcagg atacagaagg caattttgac tttgattctg tcaaagctat ctcttcggaa
     4021 gctgttctaa cttctcaatg ggatgctcaa aaattaccag ttattggggg aattgcaatt
     4081 cctgaattgg aaatgaattt gccgattttt aaaggacttg ataatgttaa tctcttctac
     4141 ggagctggta caatgaaacg cgagcaagta atgggagaag gaaattatag tctagctagt
     4201 caccatatct ttggtgttga taatgctaat aaaatgttat tttctccttt agataatgct
     4261 aaaaatggca tgaagattta tctaaccgat aaaaataaag tttatactta tgaaatacgt
     4321 gaagtcaaac gtgtgacacc ggatcgtgtt gatgaagttg atgatagaga tggggtcaat
     4381 gaaatcacat tagtaacctg tgaagacctt gctgctacag aacgtattat tgtcaaaggt
     4441 gatttgaaag aaacaaaaga ttattcacaa acatctaatg aaatcctaac agctttcaat
     4501 caaccatata aacaatttta ttaatacaaa tcagtgaaat ccatgatttc actgattttt
     4561 taagattttc ataaaaatat agtaaaatga aataagaa
//
LOCUS       DAVAAL010000135         4488 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVAAL010000135 DAVAAL010000000
VERSION     DAVAAL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.137"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            158..1417
                     /locus_tag="VQM60_001819"
     CDS             158..1417
                     /locus_tag="VQM60_001819"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET1081646.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            1433..1984
                     /locus_tag="VQM60_001820"
     CDS             1433..1984
                     /locus_tag="VQM60_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081647.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            1977..3254
                     /locus_tag="VQM60_001821"
     CDS             1977..3254
                     /locus_tag="VQM60_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET1081648.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            3270..4130
                     /locus_tag="VQM60_001822"
     CDS             3270..4130
                     /locus_tag="VQM60_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET1081649.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         1347 a          720 c         1031 g         1390 t
ORIGIN      
        1 atctttgatt tttattgagt attagtaatt cagttactaa ctcgtcaact ctgatttatc
       61 caataaaatt gaaaaggatg gaaaaaagga taaatttatg atatacttta ttttgaagac
      121 cttattagaa atcttgaaag agtattgaaa acttagaatg agaaaaattg ttatcaatgg
      181 tggattacca ctgcaaggtg aaattactat tagtggtgct aaaaatagtg ttgtggcctt
      241 aattccagct attatattgg ctgatgatgt ggtgactttg gattgtgttc cagatatttc
      301 ggatgtagcc agtcttgtcg aaatcatgga attgatggga gctactgtta agcgttatga
      361 cgatgtcttg gagattgatc caagaggtgt tcaaaatatt ccaatgcctt atggtaaaat
      421 taacagtctt cgtgcatctt actattttta tgggagcctc ttaggccgtt ttggtgaagc
      481 gacagttggt ctaccgggag gatgtgatct tggtcctcgt ccgattgact tacaccttaa
      541 ggcgtttgaa gctatgggtg ccactgctag ctacgaggga gataacatga agttatctgc
      601 taaagataca ggacttcatg gtgcaagtat ttacatggat acggttagtg tgggagcaac
      661 gattaatacg atgattgctg cagttaaagc aaatggtcgt actattattg aaaatgcagc
      721 ccgtgaacct gagattattg atgtagctac tctcttgaat aatatgggtg cccatatccg
      781 tggggcagga actaatatca tcattattga tggtgttgaa agattacatg ggacacgtca
      841 tcaggtgatt ccagaccgca ttgaagctgg aacatatata tctttagctg ctgcagttgg
      901 taaaggaatt cgtataaata atgttcttta cgaacacctg gaagggttta ttgctaagtt
      961 ggaagaaatg ggagtgagaa tgactgtatc tgaagacagc atttttgtcg aggaacagtc
     1021 taatttgaaa gcaatcaata ttaagacagc tccttaccca ggctttgcaa ctgatttgca
     1081 acaaccgctt acccctcttt tactaagagc gaatggtcgt ggtacaattg tcgatacgat
     1141 ttacgaaaaa cgtgtaaatc atgtttttga actagcaaag atggatgcgg atatttcgac
     1201 aacaaatggt catattttgt acacgggtgg acgtgattta cgtggggcca gtgttaaagc
     1261 gaccgactta agagctgggg ctgcactagt cattgctggg cttatggctg aaggtaaaac
     1321 tgaaattacc aatatcgagt ttatcttacg tggttattct gatattatcg aaaaattacg
     1381 taatttagga gcggatatta gacttgttga ggattaaacc gtagaggtgt ttatgaatat
     1441 ttggaccaaa ttagcaatgt tttctttttt tgaaacggat cgcttgtatt tgcgtccttt
     1501 cttttttagt gatagtcagg acttccgcga gatagcttca aatccagaaa atcttcaatt
     1561 tattttccca acgcaggcaa gtctggaaga aagtcaatat gcactggcca attactttat
     1621 gaagtcccct ttgggagtgt gggcaatttg tgaccagaaa aatcaacaaa tgattggttc
     1681 tattaaattt gagaagttag atgaaatcaa aaaagaagct gagcttggct attttttgag
     1741 aaaagatgct tggtcgcaag gatttatgac agaggttgtt agaaaaattt gtcagctttc
     1801 ttttgaggaa tttggcttaa aacaattatc tatcattacc caccttgaaa ataaagctag
     1861 ccaaagagtt gctcttaagt ctggatttag tttgttccgt cagtttaagg gaagtgatcg
     1921 ttacacaaga aaaatgcggg attatcttga atttcggtat gtaaaaggag agttcaatga
     1981 gtaagcatca ggaaattcta agctatttgg aggaattacc agtaggtaaa agggtcagtg
     2041 ttcgtagcat ttcgaatcat ctaggagtta gtgatggaac agcctatcgg gctattaaag
     2101 aagctgaaaa ccgtggaatt gtggagaccc gtcctagaag tggaacaatt cgtgttaaat
     2161 cccagaaagt tgctatagag agattaacgt ttgctgaaat tgcagaagtg acttcttctg
     2221 aggttctggc tgggcaagaa ggtttagaga gagaatttag taagttttca attggtgcca
     2281 tgactgaaca aaatatcttg tcttaccttc atgatggggg gctcttgatt gtcggagacc
     2341 gaacccgtat tcagttgcta gccttggaaa atgaaaatgc agttctggtt acagggggat
     2401 ttcaggttca tgatgatgtg cttaaactgg ccaatcaaaa agggattcct gttctaagaa
     2461 gtaagcatga tacctttacc gtcgcgacca tgatcaataa agccttgtca aatgtccaaa
     2521 tcaagactga tattctgaca gttgagaaac tttatcgccc tagtcatgag tatggttttc
     2581 tgagagagac agatacagtt aaagattatt tggacttggt tcgtaagaat cgtagcagcc
     2641 gtttccctgt tatcaatcaa catcaggtcg ttgttggcgt tgtaaccatg agagacgctg
     2701 gtgataaatc accaagcacg acaattgata aggttatgtc tcgtagtcta tttttggttg
     2761 gattatcgac aaatattgcc aatgtgagtc aacggatgat cgcagaagac tttgaaatgg
     2821 taccagttgt tcgaagcaat caaactttgc ttggcgttgt gacgcgacga gatgtcatgg
     2881 agaagatgag ccgttcccaa gtttcggctc taccaacttt ttctgagcag attggacaaa
     2941 agctctctta tcaccatgat gaagtagtca ttacagtgga accctttatg ctagaaaaaa
     3001 atggagtttt ggctaatggt gtattggcag aaattctgac ccacatgacc caagatttag
     3061 ttgttaatag tggtcgcaat ctcattatcg agcagatgct aatctacttt ttgcaggctg
     3121 ttcagataga tgatatattg cgcattcagg cacggattat tcatcatacg agacggtcag
     3181 ctataattga ttacgatatt tatcatggtc accagattgt ttcaaaagca aatgtgactg
     3241 ttaaaattaa ttagaaacta ggagaaaaga tgataacatt aaaatcagct cgtgaaatcg
     3301 aagctatgga caaggctggt gattttctag caagtattca tataggctta cgtgatttga
     3361 ttaagccagg cgtagatatg tgggaagttg aagaatatgt ccgccgtcgt tgtaaagaag
     3421 aaaatttcct tccacttcag attggggttg acggtgccat gatggactat ccttatgcta
     3481 cctgttgctc tcttaacgat gaagtggctc acgctttccc tcgtcattat atcttgaaag
     3541 atggtgattt gctcaaagtt gatatggttt tgggaggtcc cattgctaaa tctgacctaa
     3601 atgtctcaaa attaaacttc aacaatgttg aacaaatgaa aaaatacact cagagctatt
     3661 ctggtggttt agcagactca tgttgggctt atgctgttgg tacaccgtcc gaagaagtca
     3721 aaaacttgat ggatgtaacc aaagaagcta tgtacaaggg tattgagcaa gctgttgttg
     3781 gaaatcgtat cggtgatatc ggtgcggcta ttcaagaata cgctgaaagt cgtggttacg
     3841 gtgtagtgcg tgatttggtt ggtcatggtg ttggcccaac tatgcacgaa gaaccaatgg
     3901 ttcctaacta tggtattgca ggtcgtggac tccgtcttcg tgaaggaatg gtcttaacca
     3961 ttgaaccaat gatcaataca ggcgattggg aaattgatac agatatgaaa actggttggg
     4021 cgcataagac cattgacggt ggattgtcat gtcagtatga acaccaattt gtcattacga
     4081 aagatggacc tgttatcttg actagccaag gtgaagaagg aacttattaa taaaaagtga
     4141 aaagactact ggaagtttat tttgataaaa aatccagtag atcttttcat aataaaacgc
     4201 attgtatcaa gtgttagggg ctgatatcat gcgtttttct gcttttaaga ttttttccaa
     4261 ctctgtttgt aagcgcatca taacaaaggg tctaggattc agggctctcc tcctatatac
     4321 tattagtaaa gtaaaactaa gagaggatat tttagtgtcg cagtctattg ttcctgtaga
     4381 gattccacaa tattgtcgtt ttgattctaa aaagagaaat ggaattctgt ttaacgttcg
     4441 tattgccaat cttaaattta ccttttttag attagacttc ctgcgaaa
//
LOCUS       DAVAAL010000136         4319 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVAAL010000136 DAVAAL010000000
VERSION     DAVAAL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.138"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            468..1589
                     /gene="mnmA"
                     /locus_tag="VQM60_001823"
     CDS             468..1589
                     /gene="mnmA"
                     /locus_tag="VQM60_001823"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET1081650.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGEKTEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1730..2185
                     /locus_tag="VQM60_001824"
     CDS             1730..2185
                     /locus_tag="VQM60_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET1081651.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2195..4108
                     /gene="mnmG"
                     /locus_tag="VQM60_001825"
     CDS             2195..4108
                     /gene="mnmG"
                     /locus_tag="VQM60_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET1081652.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
BASE COUNT         1230 a          896 c         1052 g         1141 t
ORIGIN      
        1 aggtgccctt gcagtcaaca caactgtaga tggctataaa gtcaatgcca atggtgaatg
       61 ggtttaagcc gattaaatta aatcatgtta agaacatttg acattttaat tttgaaacaa
      121 agataaggtt cgattgaata gatttatgtt cgtattcttt aggtacctat cttatgattt
      181 caggaaatgt cattaaaaaa acgactcctt ttctctaact tgaaaaatag attagagaaa
      241 atgggttgtt ttatttatta tagttatttg aatgaagata agaagaaggt atactcacat
      301 cattcacata atctgtatat tgactataag ttttaaaaaa caatttttaa gctcttcctt
      361 gtcttctcta accaagcgtg ttataatgaa tactgctcaa gcgaccttca atcgtgaagc
      421 acacacgacc ttcaatcgtg aataaacgaa tagatgggag acttaccatg agtgataact
      481 ctaaaacacg tgttgtcgtg gggatgagtg gtggtgttga ttcgtcggtg acggctcttt
      541 tgctcaagga gcagggctac gatgtgatcg gtatcttcat gaagaactgg gatgacacag
      601 atgaaaacgg cgtctgtacg gcgaccgaag attacaagga tgtggttgcg gtggcagacc
      661 agattggcat tccctactac tctgtcaatt ttgaaaaaga gtactgggac cgcgtttttg
      721 agtatttcct agcggaatac cgtgcagggc gcacgccaaa tccggacgtt atgtgcaaca
      781 aggaaatcaa gttcaaggcc tttttggact atgccataac cttgggggca gactatgtag
      841 cgactgggca ttatgctcga gtggcgcgtg atgaggatgg taccgttcac atgcttcgtg
      901 gcgtggacaa tggcaaggat cagacctatt tcctcagcca actttcgcaa gaacaacttc
      961 aaaaaaccat gttcccacta ggacatttgg aaaagcctga agtacgcaga ctagcagaag
     1021 aagcaggcct ttcgactgct aagaagaaag actcgacagg gatttgcttt atcggagaaa
     1081 agaactttaa aaactttctc agcaactacc tgccagctca gcctggtcgc atgatgactg
     1141 tggatggtcg cgatatgggc gagcatgcag gtcttatgta ctatacaatc ggtcagcgtg
     1201 gcggactcgg tatcggtggg caacacggcg gtgacaatgc cccttggttc gttgtcggaa
     1261 aagatctaag caagaatatt ctctatgtag gacaaggatt ctaccatgat tcgctcatgt
     1321 caactagcct agaagccagt caagtccact ttactcgtga aatgccagaa gagtttacgc
     1381 tagaatgtac ggctaaattc cgttaccgtc agcctgactc taaggtgacc gttcatgtca
     1441 aaggagaaaa gacagaggtc atctttgcgg aaccacaacg cgcgattaca ccaggacagg
     1501 cagttgtctt ttacgatggc gaagagtgtc taggtggcgg tttgattgac aatgcttacc
     1561 gcgatggaca agtttgtcag tacatttaga ttgacaaatt ttctcaattt gctacaataa
     1621 taacagcaat agaaatgatg gtcaaagctc atggatgttg caggcttttt tgtcctgcac
     1681 ttctttgtag ttttgactgt ttttgtgtcg tttaagggaa aggacaagaa tgactcaaca
     1741 agactttcgg acaaaagtag acaatacggt ttttggtgtt cgggctacag ccttgattgt
     1801 ccaaaatcac aagcttctag ttaccaaaga caagggcaag tattacacta ttggaggtgc
     1861 gattcaagtc aatgaaagca cggaagacgc ggtagtccgt gaagtgaagg aagaactggg
     1921 tgtcaaagct caagctgggc agctagcttt tgtggttgaa aatcgttttg aagtggacgg
     1981 cgtttcctat cacaatatcg agtttcatta tctggtggat ttgcttgaag atgccccgtt
     2041 gaccatgcag gaagatgaga aaaggcagcc ctgtgagtgg attgacttgg ataagctcca
     2101 gaatatccag ctagttccag cctttttaaa aacagcccta ccagattggg aaggccaact
     2161 aagacacatt catcttgagg aataggagag aaacatgact tatcatttta ctgaagaata
     2221 cgatattatt gtaattggtg cgggacacgc tggggttgag gcttccttgg ccgctagccg
     2281 tatgggctgt aaggtcctgc ttgcgaccat caatattgaa atgctggctt tcatgccttg
     2341 taatccctct atcggtggtt ctgccaaggg gattgtcgtg cgtgaagtcg atgccctcgg
     2401 tggcgagatg gccaaaacca ttgacaagac ttacatccag atgaagatgc taaacacagg
     2461 gaaggggcca gctgtccgtg cccttcgtgc gcaggctgac aaggaacttt actctaagga
     2521 gatgcgcaag acggttgaaa accaagaaaa tctgaccctt cgtcaaacca tgattgatga
     2581 gattttggtg gaagatggca aggttgtcgg tgtgcgtaca gccacccatc aagaatatgc
     2641 tgctaaggct gttattgtga cgacagggac tgctctccgt ggggaaatta tcatcggaga
     2701 cctcaagtac tcatcaggtc ctaaccacag cttggcttct attaacctag ctgacaatct
     2761 caaggaactg ggtctcgaaa tcggtcgttt caagacagga acccctccac gtgtcaaggc
     2821 ttcttctatc aattacgatg tgacagaaat tcagccagga gacgaagtgc ctaatcattt
     2881 ctcatacact tcacgtgatg aggattatgt caaggaccaa gtaccatgct ggttgaccta
     2941 taccaatggt accagtcatg agattatcca aaacaacctc caccgtgcgc ctatgtttac
     3001 aggtgtggtc aagggagtgg ggcctcgtta ctgtccgtcg attgaagaca agattgtgcg
     3061 ctttgcggac aaggaacgtc accaactctt ccttgagcca gaagggcgca atactgagga
     3121 agtctatgtg caaggacttt caaccagtct gcctgaggat gtccagcgtg acttggtgca
     3181 ttccatcaaa ggtttggaaa atgcagagat gatgcggaca ggttatgcta ttgagtatga
     3241 tatggtcttg cctcatcagt tgcgtgcgac tttggaaacc aagaaaatct caggtctctt
     3301 cactgctggt cagacaaatg gaacatcagg ttacgaagag gcagcaggcc aagggattat
     3361 cgcgggtatc aatgcggctc tgaaaatcca aggcaagcct gaattgattt tgaagcgcag
     3421 tgatggttat atcggggtga tgatcgacga cttggtgacc aagggaacca ttgaacccta
     3481 ccgtctcttg accagtcgtg ctgaataccg tctcattctt cgtcatgaca atgctgatat
     3541 gcgcttgact gagatgggac gcgagattgg ccttgtggac gatgaacgct gggctcgttt
     3601 tgaaatcaag aaaaatcaat ttgataatga gatgaagcgc ctagacagta tcaaactcaa
     3661 gccagtcaag gaaaccaatg ccaaggttga ggagatgggc ttcaaaccct tgaccgatgc
     3721 agtgacagcc aaggaattcc ttcgccgtcc agaagtttct taccaagatg tggtggcctt
     3781 catcggacca gctgcagaag acttggatga caagattatc gaattgattg aaacagaaat
     3841 caagtatgaa ggctatattt ccaaagccat ggaccaggtt gccaagatga aacgcatgga
     3901 agaaaaacgc attccggcca atatcgactg ggatgacatt gattctatcg caaccgaagc
     3961 ccgtcagaag ttcaaactca tcaatccaga aaccatcggc caagccagcc gtatttcggg
     4021 agtaaaccca gcagatattt ctattttgat ggtgtatctg gaaggtaaaa atcgtagtat
     4081 ttctaaaact cttcaaaaat caaaatgata cgtcgtcggc ttcttacgaa tgagttcaaa
     4141 gcttggcttt gattcatctc cagcctccca tagtttcccg aactatggga gctaactcaa
     4201 gttatgcttt cgcttagcct tctagtctga tttcgatttt tattgatact cttcgaaaat
     4261 ctcttcaaac cgcgtcaacg tcgccttgcc gtaggtatgg ctactgactt cgtcagttc
//
LOCUS       DAVAAL010000137         4314 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVAAL010000137 DAVAAL010000000
VERSION     DAVAAL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.139"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            122..277
                     /locus_tag="VQM60_001826"
                     /pseudo
     CDS             122..277
                     /locus_tag="VQM60_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="VQM60_001827"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="VQM60_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET1081653.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RFYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDIDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDSFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDIERDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3749..>4314
                     /locus_tag="VQM60_001828"
     CDS             3749..>4314
                     /locus_tag="VQM60_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET1081654.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAG"
BASE COUNT         1296 a          878 c          915 g         1225 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagcaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct acccgtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagtctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cgaacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagttaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgttttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 accatgagat gaccaaatct cagatgagtt ttttggactt ggagaattta acagatgagg
      901 ataagaggac ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaagggc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc accttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgttga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcctgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatatc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagcat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga tttgtatact gacctcagtc
     1681 agtctgatat tgattctttt gagcaatata tccgctatct tggtatcaat ggcttgccag
     1741 cctttcagca aaccttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttctg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggcaaaacc taaaccaaac gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagga
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatattga gcgagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatctgca aggtctggaa aatccagcta
     2641 tcccaaccag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcgt cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttacagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacgac tggacatatt ctccgacaca atccagccat cgaaaccatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggga gcagaacttt ttcggtgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggctaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcaggc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaaattgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggt
     3721 ttgaaaaaat aagagaggag ttaaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacagaaga aaactgccga
     3841 acaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg tagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgatctca aacaacactt
     4081 gggtcgccag ttggcagacc tacccaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agagctttag tcatttgctg aaaaactttg ctgg
//
LOCUS       DAVAAL010000138         4259 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVAAL010000138 DAVAAL010000000
VERSION     DAVAAL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.140"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(12..1301)
                     /locus_tag="VQM60_001829"
     CDS             complement(12..1301)
                     /locus_tag="VQM60_001829"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET1081655.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1379..2626)
                     /locus_tag="VQM60_001830"
     CDS             complement(1379..2626)
                     /locus_tag="VQM60_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET1081656.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFWKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTVCFSLVAVWNIQLT
                     WMLFVGFSLLAIFLTILNLKNDI"
     gene            complement(2817..3668)
                     /gene="rgg"
                     /locus_tag="VQM60_001831"
     CDS             complement(2817..3668)
                     /gene="rgg"
                     /locus_tag="VQM60_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HET1081657.1"
                     /translation="MEQIGKVFRQLRESRNISLRQVTGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3792..4133
                     /locus_tag="VQM60_001832"
     CDS             3792..4133
                     /locus_tag="VQM60_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET1081658.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
BASE COUNT         1348 a          896 c          833 g         1182 t
ORIGIN      
        1 ctctttgatt ttcattgagt ataaaatcct agtttttcaa agatttctga gaagttttgg
       61 ctgattgtct caagtgacac ttgcacttct tctcgggttt ggttgttctt gaccgtcact
      121 tgtccgcttt cgacttcgct ctctcctagg gtgatgaggg tcttagccgc aaagacatct
      181 gctgacttga actgagcttt gagcttacgg ttgaggtaat cacgctctgc tttgaaacct
      241 tgttggcgaa gagcttggac tagttctagg gctttgacat ttgctccatc acccaagact
      301 gcgatataga catctagggc gttttcgata gggagggcca cgccttgttt ttcaaggatg
      361 agaagcaggc gctctacacc aagcccaaaa ccaaatccag cagtttcagg gcctccaaag
      421 taagcaacca aaccatcgta gcgaccaccc gcacagactg tcaagtcatt gccctcaatc
      481 tctgtgataa actcgaaaat ggtgtggttg tagtagtcca gaccacgcac catattggta
      541 tcgatgatgt aatctactcc aagattttcc aacatctgac gcacagcatc aaaatgagtt
      601 tggctttctt catcaagaaa gtccaagata gacggcgcat tctctactgc caccttgtct
      661 tctttttcct tagagtccaa gacacgaaga ggattttcct ccaagcgacg ttggctatcc
      721 ttagacaagg tctccttgag cggtgtcaaa tagtcaatca aggcttggcg gtaggctgca
      781 cggctctcag gatttccaag agtgttgagg tgcaatttga caccttgaat accgatttcc
      841 ttcaaaaaat gggctgccat aacgattgtt tccacatcgg tagctggatt gctagagcca
      901 aaacactcaa caccaatctg gtggaattgg cgcaagcgcc ctgcctgtgg acgctcataa
      961 cggaacatag gtcccatgta gtagaacttg cttggctttt gcacttctgg ggcgaagagt
     1021 ttattttcca cataggaacg gacaacgggc gcagttcctt ctggacggag ggtaatatgg
     1081 cggtcaccct tgtcatagaa atcgtacatt tccttggtta cgatatccgt tgtatctccg
     1141 acagagcggc tgataacctc gtaatgctcg aaaataggcg tacgcacttc tgcgtagttg
     1201 tagcgcttga aaatttcacg ggcaaagccc tcaacgtact gccacttagc agactcagca
     1261 ggtaaaatat cctgcgttcc ttttggtttt tgtaatttca tagggaatcc tctttaaact
     1321 taatagtctt attttaccat aaatagaggg attaaaacag tgagaaaaaa attaggattt
     1381 agatatcatt tttgagatta agaattgtca aaaaaatagc tagcaaggaa aaaccaacaa
     1441 atagcatcca agtcaactgt atattccata cggctactag tgaaaaacaa actgttccca
     1501 caggtatgga taaggtaaac aatagaccta aaaaattact agtacgagct agaacctctg
     1561 gagctagatt tttcatgagc atggcactaa tctttggttg aactttacca gacacataca
     1621 gagtaaagaa gagaaatagc aaaccaagca cgacttgatt gaataaatta gccaaaccaa
     1681 ctagactaag tcctacggtc gcccacatca tcaatctagg caaggactgc ttccaaaaat
     1741 aatcattgcc cgtaaggcta ctgatgatga ttgctactaa aacacagaat tgattgataa
     1801 atagtgcctc tgtataagaa aaattcaaga gagaatggct caaaaagaag atattataaa
     1861 ttccacccaa agcgccaccc aaggaattaa taagcaagac agcaaagagc ataaaaccaa
     1921 agtttttctg tccactttta agaaaaacga gacgtaaatt tcggtaaatt gttaggaact
     1981 ggtctttgat agaaagcttc tcatttttta agttttcacc atcagcagat gacattgaca
     2041 ggctcaattt gctttttcct aaaaagagga tagtggctga tactaggaaa aagcaggcat
     2101 tgattcccgc aacgagggaa aaattgttga ccgatagagc taagagccag actccgaaag
     2161 cttgaccacc aatagctgaa atataggtga tgaactgtga aaaagaataa gcctccatca
     2221 aatcatcttc agctactttt tccttaataa gaggcatacg caggccacct gcaaaatcac
     2281 tgatgatatc actaatgaca ttgatcaaac acaggctaga aaaggcaaag agactagctt
     2341 gctgaacaac tagggctgct agaaaaaata gaaccgcctg aaacaaaccg ctatagacca
     2401 tccatttgac cttgtccctc gtgtaatctg cccgaatccc tgcaaaaact gtaaagaagg
     2461 tcggaagaat catgacaata ttcgccatag caacagcaaa agatgcttgt gacaaggtcg
     2521 atgcatagac gataaagacc aggttgaaaa tcgaagcacc aaaagcattg aagaagcgtg
     2581 ataaagtcaa aattctataa gctggatttc taaacaatag tttcatagat aatctccctc
     2641 cttgttctaa gttctacatc aagaatagct ttagtatagc acttatgaat ttgcgaaacg
     2701 attcttttga atatatgcaa caaacaaaat gagcaaaaaa acgatagaac ttttgtaatg
     2761 acctgagaga gggcaacagt tctatcgctt caaatttttt cttggtttgc agatattcaa
     2821 cattcggtcg tatttctata gtattcggca gatttattac agccaagcat ctcaaaaata
     2881 cggacagcat cctccatctt tttctggcct tccttgactc taccttgctt gctatcaagg
     2941 agaccttctg cccacagata aacaattcgg aaataggtct cattttcctt gtagaaatgc
     3001 tcttcgataa cacgtttaaa ataataggca ttggtaaatt cttcacactc aatactagct
     3061 aaaaagccat tcaatagtat agtatgaaaa aggtttcgat tgccagacat ttccattaga
     3121 aaatcagatt tacgtaccat ttctcgtaca tatctagtaa aaagagaaac agataaaaat
     3181 ggagaactga ctgaaaataa attgagttca tagattcccc agatctcggt agaaaacaaa
     3241 taatcatgaa ggacttttcc ttcctctgct gttaactcta ccctttcatc tatgctcttc
     3301 atataagact tgataataat ggcatttaga atatgtttct gtttgttgtg agaatgggca
     3361 tgcttttgat actccctgcg atataagtcc tcaagaggtg ctatattctt tggttccaag
     3421 acatctgtaa tttcttttct caactcagaa tctgtatcat actggaaacc tctcgccaga
     3481 aagaggatct cctccacact ggcagatata ttttccagag caaatagaaa cttttccacc
     3541 gaaagctcac tctgacctgt ttcaaaacgg gacaacatag acggcgaaaa ttgtcctccg
     3601 gttacttgtc tcagtgagat atttcttgac tctcgtaatt gtctaaagac ttttccaatc
     3661 tgctccatag acttcccctt gattccgtat tttcttcatt ttatcatatt tttcagaaaa
     3721 ttcatcaaaa acttgccaaa ttgtcagaat tatgagaaaa tagaggatat ttatcacgtg
     3781 gagggactgc tatgagagac gatatcaaaa tcaatgaccg tgctttggcc ttgcaagacc
     3841 aaatcatcga aaaactagag aaagtttttg atacagatgt ggaattggat gtttacaatc
     3901 taggtctgat ttatgaaatc aatctggatg aaacggggct ctgcaagatt gtcatgacct
     3961 tcaccgatac tgcctgtgat tgcgccgaaa gcctgcctat tgaaatcgtg gcaggtctga
     4021 aacaaatcga gggtatcaag gatatcaagg ttgaagttac ctggtcgcct gcttggaaaa
     4081 tcacacgaat cagtcgctat ggccgtattg cccttggact accacctcgt taagcaggcc
     4141 aatcactttt aaagatgaaa aaagcaagcc gaaaatggct tgctttttga tactcaatga
     4201 aaatcaaagg gcaaactaga aaactagccg caggttgctc aaaacactgt tttgaagtt
//
LOCUS       DAVAAL010000139         4185 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVAAL010000139 DAVAAL010000000
VERSION     DAVAAL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.141"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            61..495
                     /gene="sdbB"
                     /locus_tag="VQM60_001833"
                     /pseudo
     CDS             61..495
                     /gene="sdbB"
                     /locus_tag="VQM60_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
     gene            869..2260
                     /locus_tag="VQM60_001834"
     CDS             869..2260
                     /locus_tag="VQM60_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET1081659.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            2403..3320
                     /gene="adcAII"
                     /locus_tag="VQM60_001835"
     CDS             2403..3320
                     /gene="adcAII"
                     /locus_tag="VQM60_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET1081660.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            3328..>4185
                     /gene="phtD"
                     /locus_tag="VQM60_001836"
     CDS             3328..>4185
                     /gene="phtD"
                     /locus_tag="VQM60_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HET1081661.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLS"
BASE COUNT         1294 a          751 c          884 g         1256 t
ORIGIN      
        1 gaggaagaaa ctaaaaagac tcaagcagca caacagccaa aacaacaaac gactgtacaa
       61 caaattgctg ttggaaaaga tgctccaaac ttcacattgc aatccatgga tggcaaagaa
      121 gttaagttat ctgattttaa gggtaaaaag gtttacttga agttttgggc ttcatggtgt
      181 ggtccatgca agaaaagtat gccagagttg atggaactag cggcgaaacc agatcgtgat
      241 ttcgaaattc ttactgtcat tgcaccagga attcaaggtg aaaaaattgt tgagcaattc
      301 ccacaatggt tccaggaaca aggatataag gatatcccag ttctttatga taccaaagca
      361 accaccttcc aagcttatca aattcgaagc attcctacag aatatttaat tgatagccaa
      421 ggaaagattg gaaagattca atttggtgct atcagtaatg cggatgcaga agcagcattt
      481 aaagaaatga actagaatcg ataaaatctg attacaagat gtagtcagat tttggtaact
      541 tctaaaacta acttccagtt tcccacaacc tagcttttag tatgagaaaa acgcgtgaca
      601 tcagtggaaa tccgtcttag actaacttcc actggttttc tttttcttga tatataaggg
      661 ttcaaaagcg aaaagactca gaaaaagtgc acgacaaata gccctaaaac agagtcgtct
      721 tagagtaact tccagttttg ggagagagat ggaagttact ttgagaagtt acggtagtca
      781 gattttttta gaaaaacatt ttataaatat tcacatatct cctatattta tggtaaaata
      841 gaatcatcag tttattttgg agtcaaagat gaatatattt agaacaaaga atgttagttt
      901 agataaaaca gagatgcata ggcatttgaa gttatgggat ttgattttgc tgggtatcgg
      961 agccatggta gggacaggcg tctttacaat cacaggtact gcagctgcaa cacttgctgg
     1021 cccagcccta gtgatttcaa tcgttatttc tgccttgtgt gtgggattat cagccctctt
     1081 ttttgcagaa tttgcctcgc gagtacccgc tacaggaggt gcctatagtt acctctatgc
     1141 tatcttagga gaattccctg cctggttggc tggttggtta accatgatgg agttcatgac
     1201 agccatatca ggcgtagctt cgggttgggc agcttatttt aaaggcttgc tctctcaata
     1261 cgggatagcc tttcctcagg ctttaaatgg tacctttaat cctcaagcag ggacatttgt
     1321 tgatttattg cctattcttg tcttggtctt ggtgacttcg cttgttttac taaatgccaa
     1381 ggcagcctta cgctttaatt cgattctagt tattttgaaa ttttccgctt tagctctctt
     1441 tgttttagta ggaatttgga atattaagtt tgataattgg agcaattttg ctccttacgg
     1501 ttttggacaa atctatggag ctagcactgg tattatggct ggtgcgtcct tgatgttctt
     1561 cggttttttg ggatttgaat ccatctctat ggctgtagat gaagtcaaga ctcctcaaaa
     1621 aaatattcct agagggattg tcttatcgct ttctatcgta accattctct atgccttggt
     1681 gacccttgtt ttgactggtg tggttcacta tagtcattta aatgtcgatg atgccgttgc
     1741 ctttgccctt cgtagtgttg ggattagttg ggcagccaac tatgtgtcat tagtggctat
     1801 cttgaccttg attacagttt gtatttcgat gacctatgcc ctatcgcgta tgatttacag
     1861 tttagcacgt gacggcttag tgcctgctgc ctttaaggaa ctgacgaaga ctagcaagat
     1921 accaaagaat gctactatct tgacaggtct agcttcagca gtagcagcag gaatgttccc
     1981 actagccagt attgcagcct ttttaaatat ttgtacctta gcctacttga tcatgttggc
     2041 ttatggtctg attcgcttac ggaaagaaaa aggaatgccc aaagctgggg aatttaaaac
     2101 accattggta cctctgttac caattttatc gatcatcatt tgtttatcct tcatgttaca
     2161 gtataatatg aatacctgga ttgctttcct agttgcgttg ttagtaggaa gtattattta
     2221 cttcacttat ggctataagc attctaccat agaagaataa agtgaaagtc tattgagttg
     2281 ttgacactca gcagattttt atatgtgaaa gaaatttcaa attttttcta aaaatatctt
     2341 gacagattaa attttcagga gtagaatatt tactagttaa ttaaatggtt aaggagttgt
     2401 tcatgaagaa acaaaattta tttttagtcc tgttaagtgt ctttcttttg tgcttggggg
     2461 cttgtggtca aaaggaaagt cagacaggaa aggggatgaa aattgtgacc agtttttatc
     2521 ctatctacgc tatggttaag gaagtatctg gtgacttgaa tgatgttcgg atgattcagt
     2581 caagtagtgg tattcactcc tttgaacctt cggcaaatga tatcgcagcc atctatgatg
     2641 cagatgtctt tgtttaccat tctcatacac tcgaatcttg ggcaggaagt ctggatccaa
     2701 atctaaaaaa atccaaagtg aaggtcttag aggcttctga gggaatgacc ttggaacgtg
     2761 tccctggact agaggatgtg gaagcagggg atggagttga tgaaaaaacg ctctatgacc
     2821 ctcacacatg gctagatcct gaaaaagctg gagaagaagc ccaaattatc gctgataaac
     2881 tttcagaggt ggatagtgag cataaagaga cttatcaaaa aaatgcgcaa gcctttatca
     2941 aaaaagctca ggaattgact aagaaattcc aaccaaaatt tgaaaaagcg actcagaaaa
     3001 catttgtaac acaacataca gccttttctt atctagcgaa gagatttggg cttaatcaac
     3061 ttggtattgc aggtatctct cctgaacaag aaccaagtcc acgacaacta acagaaattc
     3121 aggaatttgt taagacctat aaggttaaaa cgatttttac agaaagtaac gcttcttcaa
     3181 aagtagctga aactcttgtc aaatcaacag gtgtgggtct taaaactctg aatcctttag
     3241 agtcagaccc acaaaatgac aagacctatt tagaaaatct tgaagaaaat atgagtattc
     3301 tagcagaaga attaaagtga ggaaagaatg aaaatcaata aaaaatatct agcaggttca
     3361 gtggcagtcc ttgccctaag tgtttgttcc tatgaacttg gtcgtcacca agctggtcag
     3421 gttaagaaag agtctaatcg agtttcttat atagatggtg atcaggctgg tcaaaaggca
     3481 gaaaatttga caccagatga agtcagtaag agagagggga tcaacgccga acaaattgtt
     3541 atcaagatta cggatcaagg ttatgtgacc tctcatggag accattatca ttactataat
     3601 ggcaaggttc cttatgatgc catcatcagt gaagaacttc tcatgaaaga tccgaattat
     3661 cagttgaagg attcagacat tgtcaatgaa atcaagggtg gctatgtgat taaggtagac
     3721 ggaaaatact atgtttacct taaagatgcg gcccatgcgg acaatattcg gacaaaagaa
     3781 gagattaaac gtcagaagca ggaacgcagt cataatcata actcaagagc agataatgct
     3841 gttgctgcag ccagagccca gggacgctac accacagatg atggttatat ctttaatgct
     3901 tctgatatca ttgaggacac gggtgatgct tatatcgttc ctcacggcga ccattaccat
     3961 tacattccta agaatgagtt atcagctagc gagttagctg ctgcagaagc ctattggaat
     4021 gggaagcagg gatctcgtcc ttcttcaagt tctagttata atgcaaatcc agctcaacca
     4081 agattgtcag agaaccacaa tctgactgtc actccaactt atcatcaaaa tcaaggggaa
     4141 aacatttcaa gccttttacg tgaattgtat gctaaaccct tatca
//
LOCUS       DAVAAL010000140         4160 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVAAL010000140 DAVAAL010000000
VERSION     DAVAAL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.142"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(9..176)
                     /locus_tag="VQM60_001837"
     CDS             complement(9..176)
                     /locus_tag="VQM60_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET1081662.1"
                     /translation="MKKRILSAGLTFAAALLLAACGQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(341..1948)
                     /locus_tag="VQM60_001838"
     CDS             complement(341..1948)
                     /locus_tag="VQM60_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET1081663.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTDQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2101..3585
                     /locus_tag="VQM60_001839"
     CDS             2101..3585
                     /locus_tag="VQM60_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET1081664.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIITGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3680..>4160
                     /locus_tag="VQM60_001840"
     CDS             3680..>4160
                     /locus_tag="VQM60_001840"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET1081665.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1208 a          950 c          795 g         1207 t
ORIGIN      
        1 ctcttgtttc aattgactat attatcagcg taatagtcta atagatagtt aaatgtagtt
       61 ggatttccac taaaggttga tgagtaagtt tttgtatctg aacctgattg tccgcaagca
      121 gctaaaagca aggcagccgc aaaagtcagg cctgcactaa ggatacgctt ctttatgttc
      181 gtcttctttc tccttaatag tgggaatttg taaagttaat tgaatttcga gaatgaaagt
      241 ttataaacct tggttataaa aaacaaagga tttccgcctt ttatacagtc ctcccttgtt
      301 tttatatgat ttcaatttta aatttttctg caaaaaatat ttatagtaat tccacacaga
      361 aagcatccca tggaactaag atttgttttt caaagacttc ttgagctagg gtgttttcaa
      421 tcaagacaga tttgactttt ccttctactg tcaagtcttg ctcttcattg gacaagttag
      481 ccacaactag gaagcgacgg tcgccatcct tacgtatata agcaaagacc ttatcagccg
      541 tatcaagcaa ttcaaagtca gctcgaatta gccaactatt ctccttgcga atttggacca
      601 gtttctgata ggtatagaaa atagaatctg gatttgccag cgcttcttgg acgttgatca
      661 tctcgtaatt tggattaact gccaaccaag gttgatctgt tgagaaacca gcgtttttgc
      721 tctcgtccca ttgcataggg gtacgggcat tgtcacgtcc aataacacgg atactgtcca
      781 tgatttcttc aatcggaaca cctttttcaa gagcctcacg cgcatagttg agagattcaa
      841 tatcttctac ttgatccagt gtttcaaacg gatagttggt catcccaatc tcctcacctt
      901 ggtagatata aggagttcct ctcataagat gaagcaagat tgcaaaggct ttggcagatt
      961 tttcgcggta ttcttggtca tttccccaga ttgagacgat acgagggagg tcatgattgt
     1021 tccagaagag ggaattccaa ccgtcctcaa ctcctaactc tgtctgccat ttgttgaaga
     1081 tttcttttaa cttagcgata ttcagctctt tttgatagtg ccatttaggc tgaccttcct
     1141 gatactgaag accgatatgt tcaaactgga agaccataga caattcttgc ccctttggat
     1201 cagagtaaag cttggcaatc tctggcgttg ctccccaagt ctcccctact gtcaagagat
     1261 ccttatctcc aaaggtcgcc tgattcattt ccttgagata gggatggagc ataggaccat
     1321 tattgactac cttctcgtca ggaattttgc caatcatgtc aataacatcc atacggaaac
     1381 caccaatacc tttatcaatc cagaagttca tcatctcata aattttctgg cgaagttttt
     1441 cattttccca gttgagatcc ggctgtttct tgctgaaaaa gtggagatag tattgacctg
     1501 acttttcatc gtattcccaa gcagacccac taaagataga atctaggtca ttgggttcat
     1561 cgcgccagat atagtagtct cgctcagggc tgtcagtatt ttcacaggct tcgacaaacc
     1621 aagcatgttc atctgaggta tgattaacca ccaagtccat gatgatacga atgtcacgct
     1681 tcttagcttc tgcaatcagc tgatccatgt cctccatggt tccaaaaata gccgcaatcg
     1741 cttgataatc agcaatatca tagccattat catccatagg gctgtcataa acgggagaaa
     1801 gccaaattgc tgtgattcct agcttggcta gatagtccaa cttactggta atacctggca
     1861 aatcaccaac tccatctcca ttactatcca taaaactctt tggatagact tgatagacta
     1921 cggcattgtg ccaccatttt tcttgcatct gtttacctca ttattttaat aatctatagt
     1981 atatgatagc actttttaaa attttgtgca agcgtttgct taatcttttt aatttatttt
     2041 ttagctccgt agtttcctaa cttccaattt tttattataa tagagtccag aggtaaaaaa
     2101 atgaaaaaac aagcttttag ttctgaacag tatttgaatt tacaacgcga ccacatctta
     2161 gagcgcatta accaatttga cggcaagctt tacctagagt ttggtggcaa aatgttagaa
     2221 gatttccacg ctgctcgtgt ccttcctggt tatgaacctg acaacaaaat caagctcttg
     2281 caagaattga aagagcaggt tgaggttgtg attgccatta atgctagcaa cattgaacat
     2341 tccaaagcac gtggcgactt gggcatttct tatgaccaag aagttcttcg tttgattgac
     2401 aaattcaatg aattagggat ttttgttggt tccgttgtca ttacacaata cgctggccaa
     2461 cccgctgcag atgccttccg caaccaactt gagaaaaacg gaattgattc ttatcttcat
     2521 tatccaatca aaggatatcc gacggatatg gatcacatca tttccccaga aggcatgggc
     2581 aaaaacgact acatcaaaac cagtcgcaac ttgattgtcg taaccgctcc tggacccggt
     2641 tctggaaaat tggcaacgtg tatgtccaat atgtaccacg accaaatcaa tggcatcaaa
     2701 tctggctacg ctaaatttga aaccttccct gtttggaatc ttccccttca tcatccagtt
     2761 aacttggctt atgaagctgc cacagctgac cttgatgatg tcaacatgat tgaccccttc
     2821 catcttcaaa cctatggaga aaccactgtc aactacaacc gtgatatcga aatcttccca
     2881 gtgctcaagc gcatgttgga acgtattctc ggaaaatcac catacgcttc accgacagat
     2941 atgggtgtca acatggttgg tttcgctatt acagatgacg aggctgctgt cgaagcttct
     3001 aaacaagaaa tcatccgccg ttactatcaa acagttcttg acttcaaagc tgaaaaagtt
     3061 ggcgaagctg ccgtcaagaa aattgagttg ctcatgaacg acctcggtat cacacctgca
     3121 gaccgtaagg ttgctgtcgt tgcgcgccaa aaagcagaag aaactggtgg accagcccta
     3181 gcctttgaat tgccaaatgg ggaaatcatc actggtaaga actcagaact ctttggccct
     3241 acagccgctg ccttgatcaa tgccatcaaa aaatcagctg acatcgctaa agaagtaaaa
     3301 ctaatcgagc ctgaagttgt taagccaatc caaggtctta aaatcgatca tctcggtagc
     3361 cgcaatccac gccttcattc aaatgaaatc ctgattgcac ttgctatcac agctacagaa
     3421 aatcctgatg ctgcccgcgc tatggaagaa ctcggcaacc tcaaaggaag cgaagcccac
     3481 tcaaccatca tcttgactga tgaagacaag aatgtccttc gtaaactggg tatcaacgta
     3541 acctttgacc catactacca atacgaccgc ttatatcgta agtaaagatt tcaacctctc
     3601 cagcctcgga gaggttttct ctctgtctca ggagcaacct ttatgatata atgagagaag
     3661 aaaactgaaa ggatttacca tgtcaaaaga agttattgtc gaaagttttg aacttgacca
     3721 caccattgtc aaagcaccct atgttcgctt gattggggaa gaaacaggac caaaaggaga
     3781 catcatctcc aattatgata ttcgcttggt gcaaccaaac gaagactcta tccctactgc
     3841 cggccttcac actatcgagc acctcttagc caaactcatc cgtacccgca ttgacggcat
     3901 gattgactgt tcaccatttg gttgccgcac aggcttccac atgattatgt ggggacgtca
     3961 caccagtgct aaaatcgcag ctgttatcaa ggattcgctc aaggaaatcg ctgaaactac
     4021 tacttgggaa gatgtcccag ggacaaccat cgaatcttgc ggaaactaca aggaccacag
     4081 cctcttctct gctaaagaat gggcaaaact catcttggaa caagggattt cagatgatgc
     4141 ctttgaacgt catgtgattt
//
LOCUS       DAVAAL010000141         4136 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVAAL010000141 DAVAAL010000000
VERSION     DAVAAL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.143"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            720..1712
                     /locus_tag="VQM60_001841"
     CDS             720..1712
                     /locus_tag="VQM60_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET1081666.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="VQM60_001842"
     CDS             1714..2532
                     /locus_tag="VQM60_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET1081667.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="VQM60_001843"
     CDS             2534..3319
                     /locus_tag="VQM60_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1081668.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3417..3635
                     /locus_tag="VQM60_001844"
     CDS             3417..3635
                     /locus_tag="VQM60_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET1081669.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3620..3817
                     /locus_tag="VQM60_001845"
     CDS             3620..3817
                     /locus_tag="VQM60_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081670.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFLQLWNPQNPCQILIKKLE"
BASE COUNT         1153 a          702 c          894 g         1387 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat taatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta caaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cggtgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tatttcatga
      541 gagaaatcct aatttcacaa tctataggca aacgcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttctgtagta ataaaatgta gaaggtgtag aaagtaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aacattttgt gaagattgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccaaact cgctctttca taagcgtatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg agcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca aaagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgaag gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctatc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gttaggatta
     2161 actgtccttt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tctccttttt gcctttttcg tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttaatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggagagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccaa aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagga acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaggc agttgtcgtt gaggttttga
     3421 aggatttgcc agaatggttt ggaatcccag aaagcacaca agcctatata gaaggaacca
     3481 cgacactgca agtttggacc gcctatcagg agagtgattt gactggattt gtaaggttat
     3541 cctattcgag tgaagattgt gcagagattg attgtctcgg cgtaaaaaag cttatcaagg
     3601 tagaaaaatt gggagccaat tgcttgctac tttagagagt gaagctcgta aaaaagttgg
     3661 ttatctgcag gtcaaaacag tggcagaagg ttctaataaa gattatgatc gaacaaatga
     3721 cttttatcga ggtcttggct ttaaaaagtt agagattttt cttcaactat ggaatccgca
     3781 aaatccttgt cagattttga ttaaaaagct tgaataatat tacttgacat ctattctcag
     3841 agtgctatac tgtaagtgta atcgccgatt tagcttagtt ggtagagcaa ggcactcgta
     3901 aagcctaggt tataggtaga taaacgactg aggatttgaa aaaatagata ggtagaagat
     3961 aaccgttaag ccttactctt agcggttatt tatattgttt aatagcgctg atatttcatc
     4021 aattatgcct gttttcgtgt ttctggtagt tgttcaagtt tattgctact atttttgatg
     4081 gtatgaatgt gcttataatg tatcccggtt aacgaaagtt ttggacttat actctt
//
LOCUS       DAVAAL010000142         4126 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVAAL010000142 DAVAAL010000000
VERSION     DAVAAL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.144"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            371..598
                     /locus_tag="VQM60_001846"
     CDS             371..598
                     /locus_tag="VQM60_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081671.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            880..3777
                     /gene="nanA"
                     /locus_tag="VQM60_001847"
     CDS             880..3777
                     /gene="nanA"
                     /locus_tag="VQM60_001847"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET1081672.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYGNYNDAPLKVKPGQWNSVTFTVEKPTPE
                     LPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAVLTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKAFD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1406 a          681 c          896 g         1143 t
ORIGIN      
        1 gtcagtctta tccacaacct caaaacagtg ttttgagcat catgcggcta gcttcttagt
       61 ttgctctttg attttcattg agtataaaaa cagatgagtt tctgttttct ttttatggac
      121 tataaatgtt cagctgaaac tactttcaag gacattatta tataaaagaa ttttttgaaa
      181 ctaaaatcta ctatattaca ctatattgaa agcgttttaa aaatgaggta taataaattt
      241 actaacactt ataaaaagtg atagaatcta tctttatgta tatttaaaga tagattgctg
      301 taaaaatagt agtagctatg cgaaataaca gatagagaga agggattgaa gcttagaaaa
      361 ggggaataat atgatattta aggcattcaa gacaaaaaag cagagaaaaa gacaagttga
      421 actacttttg acagtttttt tcgacagttt tctgattgat ttatttcttc acttatttgg
      481 gattgtcccc tttaagctgg ataagattct gattgtgagc ttgattatat ttcccattat
      541 ttctacaagt atttatgctt atgaaaagct atttgaaaaa gtgttcgata aggattgagc
      601 aggaagtatg gtataaatag cataagctga tgtccatcat ttgcttataa agagatattt
      661 tagtttaatt gcagcggtgt cctggtagat aaactagatt ggcaggagtc tgattggaga
      721 aaggagaggg gaaaattggc accaatttga gatagtttgt ttagttcatt tttgtcattt
      781 aaatgaactg tagtaaaaga aagttaataa aagacaaact aagtgcattt tctggagtaa
      841 atgtcttatt tcagaaatcg ggatatagag aggatcagta tgaatcggag tgttcaagaa
      901 cgtaagtgtc gttatagcat taggaaacta tcggtaggag cggtttctat gattgtagga
      961 gcagtggtat ttggaacgtc tcctgtttta gctcaagaag gggcaagtga gcaacctctg
     1021 gcaaatgaaa ctcaactttc gggggagagc tcaaccctaa ctgatacaga aaagagccag
     1081 ccttcttcag agactgaact ttctggcaat aagcaagaac aagaaaggaa agataagcaa
     1141 gaagaaaaaa ttccaagaga ttactatgca cgagatttgg aaaatgtcga aacagtgata
     1201 gaaaaagaag atgttgaaac caatgcttca aatggtcaga gagttgattt atcaagtgaa
     1261 ctagataaac taaagaaact tgaaaacgca acagttcaca tggagtttaa gccagatgcc
     1321 aaggctccag cattctataa tctcttttct gtgtcaagtg ctactaaaaa agatgagtac
     1381 ttcactatgg cagtttacaa taatactgct actctagagg ggcgtggttc ggatgggaaa
     1441 cagttttacg gtaattacaa cgatgcaccc ttaaaagtta aaccaggtca gtggaattct
     1501 gtgactttca cagttgaaaa accgacacca gaactaccta aaggccgagt gcgcctctac
     1561 gtaaacgggg tattatctcg aacaagtctg aaatctggca atttcattaa agatatgcca
     1621 gatgtaacgc atgtacaaat cggagcaacc aagcgtgcca acaatacggt ttgggggtca
     1681 aatctacaga ttcggaatct cactgtgtat aatcgtgctt taacaccaga agaggtacaa
     1741 aaacgtagtc aactttttaa acgctcagat ttagaaaaaa aactacctga aggagcggtt
     1801 ttaacagaga aaacggacat attcgaaagc gggcgtaacg gtaaaccaaa taaagatgga
     1861 atcaagagtt atcgtattcc agcacttctc aagacagata aaggaacttt gatcgcaggt
     1921 gcagatgaac gccgtctcca ttcgagtgac tggggtgata tcggtatggt catcagacgt
     1981 agtgaagata atggtaaaac ttggggtgac cgagtaacca ttaccaactt acgtgacaat
     2041 ccaaaagctt ttgacccatc gatcggttca ccagtgaata tcgatatggt gttggttcaa
     2101 gatcctgaaa ccaaacgaat cttttctatc tatgacatgt tcccagaagg gaagggaatc
     2161 tttggaatgt cttcacaaaa agaagaagcc tacaaaaaaa tcgatggaaa aacctatcaa
     2221 atcctctatc gtgaaggaga aaagggagct tataccattc gagaaaatgg tactgtctat
     2281 acaccagatg gtaaggcgac agactatcgc gttgttgtag atcctgttaa accagcctat
     2341 agcgacaagg gtgatctata caagggtaac caattactag gcaatatcta cttcacaaca
     2401 aacaaaactt ctccatttag aattgccaag gatagctatc tatggatgtc ctacagtgat
     2461 gacgacggga agacatggtc agcgcctcaa gatattactc cgatggtcaa agccgattgg
     2521 atgaaattct tgggtgtagg tcctggaaca ggaattgtac ttcggaatgg gcctcacaag
     2581 ggacggattt tgataccggt ttatacgact aataatgtat ctcacttaaa tggctcgcaa
     2641 tcttctcgtg tcatctattc agatgatcat ggaaaaactt ggcatgctgg agaagcggtc
     2701 aacgataacc gtcaggtaga cggtcaaaag atccactctt ctacgatgaa caataaacgt
     2761 gcgcaaaata cagaatcaac ggtggtacaa ctaaacaatg gagatgttaa actctttatg
     2821 cgtggtttga ctggagatct tcaggttgct acaagtaaag acggaggagt gacttgggag
     2881 aaggatatca aacgttatcc acaggttaaa gatgtctatg ttcaaatgtc tgctatccat
     2941 acgatgcata atggaaaaga atacattatc ctcagcaatg caggtggacc gaatcgtgaa
     3001 aatgggatgg tccacttggc acgtgtcgaa gaaaatggtg agttgacttg gctcaaacac
     3061 aatccaattc aaaaaggaga gtttgcctat aattcgctcc aagaattagg aaatggggag
     3121 tatggcatct tgtatgaaca tactgaaaaa ggacaaaatg cctataccct atcatttaga
     3181 aaatttaatt gggaattttt gagcaaagat ctgatttctc ctaccgaagc gaaagtgaag
     3241 cgaactagag agatgggcaa aggagagatg ggcaaaggag ttattggctt ggagtttgac
     3301 tcagaagtat tggtcaacaa ggctccaacc cttcaattgg caaatggtaa aacagcgact
     3361 ttcctaaccc agtatgatag caagaccttg ttgtttgcag tagataagga agatatcgga
     3421 caggaaatta ttggtatagc taaaggaagc atcgaaagta tgcataatct tcctgtaaat
     3481 ctagcaggtg ccagagttcc tggcggagta aatggtagca aagcagcggt gcatgaagtt
     3541 ccagaattta cagggggagt taatggtaca gagccagctg ttcatgaaat cgcagagtat
     3601 aagggatctg attcgcttgt aactcttact acaaaagaag attatactta caaagctcct
     3661 cttgctcagc aggcacttcc tgaaacagga aacaaggaga gtgacctcct agcttcacta
     3721 ggactaacag ctttcttcct tggtctgttt acgctaggga aaaagagaga acaataagag
     3781 aagaattcta aacatttgat tttgtaaaaa tagaaggaga tagcaggttt tcaagcctgc
     3841 tatctttttt tgatgacatt caggctgata cgaaatcata agaggtctga aactactttc
     3901 agagtagtct gttctataaa atatagtaga ttgaaataag atgtgaacaa ctctatcagg
     3961 aaagtcaaat taatttatag aaaaatttta gcagtcaagg tgtactgtta tagattcaat
     4021 atattatgtg actattaacc ttctcttctc ctaaaattga ctttcttgtt ttcttatctt
     4081 gtccactcga aacaagtatt gtaagaattt gattattttt gaaagt
//
LOCUS       DAVAAL010000143         4083 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVAAL010000143 DAVAAL010000000
VERSION     DAVAAL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.145"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..945)
                     /locus_tag="VQM60_001848"
     CDS             complement(<1..945)
                     /locus_tag="VQM60_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET1081673.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKT"
     gene            1175..1864
                     /gene="coaB"
                     /locus_tag="VQM60_001849"
     CDS             1175..1864
                     /gene="coaB"
                     /locus_tag="VQM60_001849"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET1081674.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISTDQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            1876..2427
                     /gene="coaC"
                     /locus_tag="VQM60_001850"
     CDS             1876..2427
                     /gene="coaC"
                     /locus_tag="VQM60_001850"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET1081675.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLITPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            2411..2974
                     /locus_tag="VQM60_001851"
     CDS             2411..2974
                     /locus_tag="VQM60_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET1081676.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            3284..3811
                     /locus_tag="VQM60_001852"
     CDS             3284..3811
                     /locus_tag="VQM60_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET1081677.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            3823..>4083
                     /locus_tag="VQM60_001853"
     CDS             3823..>4083
                     /locus_tag="VQM60_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET1081678.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLA"
BASE COUNT         1232 a          903 c          720 g         1228 t
ORIGIN      
        1 tgtcttgata tcaaggaatt tctcagcacc aaggtccgca ccaaaaccag cttcagtaac
       61 agtgtaatca gccaagtgaa gggctgttgt cgtcgccaaa acagagttac agccatgagc
      121 gatattggca aatggaccac cgtgtacaaa ggcaggtgta ccgtaaattg tctgaaccaa
      181 gtttggctta atagcatcct tcaaaatcaa agccaaggca ccctcaacct gcaaatcacc
      241 tacagaaaca ggcgtacggt catagcgata accaataacg atattcgcca aacgacgttt
      301 caagtcctcg atgtccgttg ccaagcaaag aattgccatg atttctgaag caactgtaat
      361 atcaaaacca tcctcacgtg gaataccgtt tagaggacca ccaagaccaa cagtcacatg
      421 gcggagcgca cggtcgttca agtccacaac gcgtttccag aggatacgac gttgatcaat
      481 tcccagctca ttcccttggt gcaagtggtt gtcaatcaag gcagaaaggg cattgttggc
      541 agttgtaata gcatgcatat ctccagtaaa gtggaggttg atgtcttcca ttggcagaac
      601 ttgtgcatac ccaccaccag cagcaccacc cttgatcccc atgactggac caagagacgg
      661 ttcgcggata gcaatcatgg ttttcttgcc aatcttgttc aaggcatccg caagaccaat
      721 ggtaagcgtc gactttcctt cacctgcagg tgttgggttg atggcagtaa ccaagatcaa
      781 tttaccgact ggattgctct caactgcacg aattttatca aagctgagtt tagccttgta
      841 ctttccgtac aactccaaat cgtcgtaaga aataccaagt ttctctacaa catcaacaat
      901 tggcttcaac tcaatactct gtgcgatttc aatatctgtt ttcattcaaa attcctctaa
      961 cctcttatat gataattcat tatatcacaa aacaagattt ttaacatcct aaaactctct
     1021 aaacgttcgt aaatatctct gtttttaaga cttttagagt cctttcttaa attttatatg
     1081 gctttatagt ttgaaactat aataaatttt cgtttttacc aaaaatttat cactttcatt
     1141 ttacttaccg cttatttttg tgtacaatag tgctatgaaa attttagtta catcgggcgg
     1201 taccagtgaa gctatcgata gcgtccgctc tatcactaac cattctacag gtcacttggg
     1261 gaaaattatc acagagactt tgctttttgc agggcatgaa gtttgtttaa ttacgacaaa
     1321 acgagctctg aagccagagc ctcatcctaa cctaagtatt cgagaaatta ccaataccaa
     1381 ggaccttcta atagaaatgc aagaacgtgt tcaggattat caggtcttga tccactcaat
     1441 ggctgtttct gactacactc ctgtttatat gacagggctt gaggaagttc aggctagctc
     1501 caatctaaaa gaatttttaa gcaagcagaa tcatcaggcc aagatttctt caactgatga
     1561 ggttcaggtt ttgttcctta aaaagacacc caaaatcata tccctagtca aggaatggaa
     1621 tcctactatt catctgattg gtttcaaact gctggttgat gttaccgaag atcatctggt
     1681 tgacattgca cgaaaaagtc ttatcaagaa tcaagcagat ttaatcatcg cgaatgacct
     1741 gactcaaatt tcaacagatc agcaccgagc tatatttgtt gagaaaaatc agcttcaaac
     1801 agtccagact aaagaagaaa ttgcagaact cctccttgaa aaaattcaag cctatcattc
     1861 ttagaaagga aaactatggc aaacattctc ttggctgtaa cgggttcaat cgcctcttat
     1921 aagtcggcag atttagtcag ttctctaaaa aaacaaggcc atcaagtcac tgtcttaatg
     1981 actcaggctg ctacagagtt tatccaacct ttgacactac aggtactctc acagaatcct
     2041 gtccacttgg atgtcatgaa ggaaccctat cctgatcagg tcaatcatat cgaacttgga
     2101 aaaaaagcag atttatttat cgtggtacct gcaactgcta acactattgc aaaactagct
     2161 cacggatttg cggacaacat ggtaaccagt acagctctag ccctaccaag tcatattccc
     2221 aaactaataa ctcctgctat gaatacaaaa atgtatgacc atccagtaac tcagaataat
     2281 ctgaaaacat tagaaaccta cggctatcag ctgattgctc ctaaggaatc cctactagct
     2341 tgtggagacc acggacgagg agctttagct gacctcacaa ttattttaga aagaataaag
     2401 gaaactatcg atgaaaaaac gctctaatat tgcacccatt gctatctttt ttgctaccat
     2461 gctcgtgata cactttctga gctcacttat ctttaacctt tttccatttc caatcaaacc
     2521 gaccattgtt catattcctg tcattattgc cagcattatt tatggtccac gagttggggt
     2581 tacacttgga tttttgatgg gattacttag cttgacggtt aacacgatta cgattctacc
     2641 gacaagctac ctcttctctc ccttcgtacc aaacggaaac atctactcag ctatcattgc
     2701 cattgtccca cgtattttga ttggtttaac tccttactta gtctataaac tgatgaaaaa
     2761 caagactggt ctgattttag ctggggccct tggttccttg acaaatacta tctttgtcct
     2821 tggaggaatc ttcttcctat ttggaaatgt ttataatgga aatatccaac ttcttctggc
     2881 aaccgttatc tcaacaaatt caattgctga attggtcatt tctgcaattc taaccctagc
     2941 cattgttcca cgactacaaa ccttgaaaaa ataaaaacaa tctccgcttt caaacctgaa
     3001 ggtggagatt ttgctctata cagtttcttt ttaatgaaat ctttactata gtaaaacgaa
     3061 ataagaacag gacaaattga acaggacaaa ttgaacagga caaatcgatc aggacagcca
     3121 aatcgatttc taacaatgtt ttagaagtag aggtgaacta ttctagtttc aatctactat
     3181 aacttttaac taaaaaatga tctataaacc caagtaaatt cttgctttat tgtctggttt
     3241 attgtaccat actagtgtat atgcagttaa aaaggagatt cttgtgaata cacggaaaaa
     3301 gacacaattt atgacaatga cagccctttt aacggctatt gcgattttga ttccaattgt
     3361 tatgcctttc aagattgtca ttccacctgc ttcctatact ttggggagcc acatcgctat
     3421 ttttatagcc atgttcttgt cgcccttgat ggcagttttt gtcatcctag cctctagttt
     3481 tggatttttg atggctggct atcccatggt tatcgttttt agggcttttt cccatatatc
     3541 ttttggtgct ttaggagctc tttacctaca aaaattcccc gataccctag ataaaccaaa
     3601 atcttcctgg gttttcaact ttgttttggc tgttgttcat gcccttgctg aagtattagc
     3661 ctgtgtcctt ttttacgcaa cttcgggtac caatgtagaa aacatgttct atgttctatt
     3721 tgtactagtt ggatttggca caattatcca tagtatggta gactatacat tagcactagc
     3781 tgtctataaa gtgcttcgaa aacgccgtta aaaggaaaag ctatgacaaa agatcgcaaa
     3841 caagccctgc tccaactctt gaaagaagct cctaaagccc ttaatggcca aagtttggcg
     3901 gaacattttc atgtcacacg tcaggtcatt gtgcaggaca ttgcaatttt gagagccgat
     3961 ggcgctccta tcctatccac caatcgtggc tacatctata agcaaattga taccaatcct
     4021 tatgttcaca aacttttcaa agtgaaacat gaagttgaag aaatcggtca ggaactcctt
     4081 gct
//
LOCUS       DAVAAL010000144         4067 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVAAL010000144 DAVAAL010000000
VERSION     DAVAAL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.146"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(60..659)
                     /locus_tag="VQM60_001854"
     CDS             complement(60..659)
                     /locus_tag="VQM60_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1081679.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSVADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(661..1758)
                     /locus_tag="VQM60_001855"
     CDS             complement(661..1758)
                     /locus_tag="VQM60_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET1081680.1"
                     /translation="MLERLKSIHYMFWTSLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGIMLVYVAGNTAFVAGNYIWFFFFLSNILIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKIDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTIR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(1758..2495)
                     /locus_tag="VQM60_001856"
     CDS             complement(1758..2495)
                     /locus_tag="VQM60_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET1081681.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GEPANFMRDYLISMTAFSMMSTTIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(2497..3381)
                     /locus_tag="VQM60_001857"
     CDS             complement(2497..3381)
                     /locus_tag="VQM60_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081682.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(3368..3562)
                     /locus_tag="VQM60_001858"
     CDS             complement(3368..3562)
                     /locus_tag="VQM60_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081683.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(3559..3765)
                     /locus_tag="VQM60_001859"
     CDS             complement(3559..3765)
                     /locus_tag="VQM60_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081684.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         1306 a          912 c          679 g         1170 t
ORIGIN      
        1 tattgttcca ttcactaccg tcaattttag cacatagtct tcatgaaaat attatatcat
       61 cataaccaac cagattcttt tgcgatatta gctgcctctg ttcgattacc agcatccaat
      121 tttgaaagaa tattggtgac atagtttcga atagttccgt ttgagaggta aagctgatct
      181 gcgatttctt ggttggataa gccccgggcg atccccttta agacagcaat ctcttgttct
      241 gtcaacggat tgggacgggt catcaccact tccatcaatt caggcgaata ctccttgcgt
      301 ccttcgagga cggtgtgcaa ggtttgcatg aggtctgcaa cgcttcgttc ctttaataca
      361 taagcatcca ctccagcctt gaccgcacgt tcaaaatacc cagcacgctt gaaggtcgtc
      421 accacaacca cctttgtttc aagcctttct gatcgtatcc actccaagac ttcaagacct
      481 gtcttaacag gcatttctac gtcaaggatg gcgatatcta cagactcctt ttctaatagt
      541 tggattgctt cttgcccatt cttggcttga aggacagact ccacatctgg ttgaagcgtg
      601 agtaattgac acatggcatc tcgcaacata ctttgatctt ctgcgactaa tactttcatc
      661 ttagtttctc tccttataag gtagtcgaac ttgcacttcc gttggatgtt tctgactgat
      721 tacacttact tctcctgaaa atggaaaaac acgatctcgg attgtatgga gctcatctcc
      781 ttttagaaaa gcaaagccgc agccatcatc acttactgtt aaaatgagtt ccttctctgt
      841 ccgttctaat tttaagtaag ctttagacgc tttggcatgt ttgatgatat tggtcaccaa
      901 ctcaagcaaa atcatagaag ccattgactc caattcttga gtaaggctag cagtatctag
      961 ttggttatct gtttccacct caattccagc aatttctaac atctttttca cagtctctag
     1021 ttcggatgtc aaagttctag acttaagatt ttccacaatg gttcgaactt cacgcattga
     1081 ttctttgcta atttgctgta tttctcttaa ttccttttcc acttgtggat aagcctgcat
     1141 ttgaaatagc tgcaaggcta gatctatctt gacactaagc atagcaaagg tatgtcccag
     1201 actatcatgc aaatcctgac cgatacgatt gcgctcattt tctgctagca atagatttat
     1261 ctgggtattt tgcttgacct gagcttcttt caaatcctcg acaaggcgaa ttcgaaccga
     1321 gcccaaagtc atcaaatcga caaaagtaag aattacaagc atatagacta gaaactcaac
     1381 ttcgattctc tgaaaaatca aaagtcgccc cacaacaagg acttgagcaa ggagaaatgt
     1441 ccatacatgt aaagacttta aactacgtac gccaaaatga taaattaaga tattggaaag
     1501 gaaaaagaaa aaccagatat aattcccagc aacaaaggcg gtattcccag ctacatagac
     1561 cagcatgatc ccccagaaaa gccaagatag gcgctggttc ttagttatta aaacacccaa
     1621 atacgtcact acaaatagaa tatcaaccaa taaatgccaa gcaggaagct ccccaatcac
     1681 tacaggtagg atgggaaaaa gcataaaaat caaactggtc caaaacatat agtgtatgct
     1741 tttcagtctt tcaagcatta agcattctcc ttatgacctt gaaggtaaat agtcaaacca
     1801 aataaaactg ctgaaaaaac aagtaaataa actgtggctg atagattgat gccaccctcg
     1861 tttaagaagg tcttgagcaa ctccatcaat tgataggtcg ggagacactt accaactgct
     1921 tgcatccagt ctggaaataa agaaatgggc atccagagtc cgcctaaaac agccaatcct
     1981 agataaagaa gattgcccac aacagacatc agctgactag ttggtaagag agtcaaggtc
     2041 aagccaagtg ctacaaaagc aatactcccc acaatcagca aaatcgcagc tccaacccat
     2101 tttactagag gcatgtctac acctcttaca aaatgtccaa ctgtaaaaac aaccagaatt
     2161 gagaccaaat aatcaaccag catacttgtt atctttgata gataatattc taccatattt
     2221 acagggctat gacgcaatgt tttctgccag ttgttcatct tatcggtatg taaaacagct
     2281 ggaaatgaga agatagttgt cgacatcatg gaaaaggcag tcatggagat gaggtaatca
     2341 cgcataaaat tcgcgggttc acctggtgtg tcctgataga taccagaaaa aaataaatag
     2401 aaagccgtcg gcatccctac cgacaataga taatagatta attgtcgttt ggtcaataaa
     2461 aattctatct tactaagtgc tatccatcgt ttcatcttag ttatctccct tctgcgtctc
     2521 ttcaaagatt gtattcaaca aactacgatt attaacttcg atttcttgca tcctacatcc
     2581 tgcttgggct aatagttccc agaaagcatc tgcttctcga gtgactactt gtaaagaatc
     2641 ttgctttagg gtccagtttt caaccaaatt tgactgctct acgacttcct tgtaagctat
     2701 aggaaggata aagtgctttt ctatttcctc gctacgcata gctagaggcg tcgtatctcg
     2761 aatcaactct cccttattta agagcaaaat gcggtctgca gtgtgttcca cttcctcaat
     2821 ataatgggac gaatagagaa tggtgactcc ttgcgctttt aactcctgga caatttccca
     2881 aaaacgttga cgagttgagg tatccatgga cgcagtaggc tcatctaaaa aaacaatctt
     2941 tggtcgccca attaaggtca agacgaaaga aaagagacgc ttttgaccgc ctgataattt
     3001 ttctgccaac tgttcttttt gttgcttgac aaactgcaat agttgatcaa tttcctgatt
     3061 gctcaaagga tttggataga tacgttgaaa gaaagcaatc aactctttaa cttttaattt
     3121 ctgaacgatt acattttctt gagggaggta agccctagta tagtctaact tagaactcgt
     3181 cactggtaaa ccttgaatag atacttgacc gcttgtgacc agtttatctc caagcaaaca
     3241 atctaagagt gttgtctttc cggcaccatt tggaccaatt aaggcaacac attcaccatc
     3301 gttgatttca aaggagatgt tccgcaaaat ttccttgtct tttattttct tactcaattt
     3361 ctcaacttta accacagtca tgagactctc ctttcaacca ctccattccc atagggaaaa
     3421 cgacgaaaat cataaatcca aaccccaaag caccacaaat gaattggcga agcaaggctt
     3481 gatcaaacca atcagtaaac atttccacta accataccaa gagtgacagg ccgataaaga
     3541 aatagatgat ccctctcttc attcttcaaa ctcctttttc acatctctga ctaatttcaa
     3601 accttctcta acaagccaag acatgattcc aaagccagca aagaccttcc aaggaaaatg
     3661 gtagaaatcc tcatccaatc ccgaaaacat gagataggtc ataactcctg ctactactaa
     3721 actcactgcg ataatcattt tatttctcat ctcttcttcc cccatttcat actacaatta
     3781 tagtctttta aaatcagagg aggcagaagc ttctgtcact agaaaatatg acaaatgtca
     3841 taaaaaattt tgttcaaaac aagcaagata cactatacaa taaaacacaa tcagaaaaat
     3901 ctaaggcaac ttcctcaaaa gagatatcaa acccaattca caccataatg taaactaata
     3961 cttatttaaa atcaaaaaga gtagaaattt ttatcagaca aacacatata tagtgtattg
     4021 aatctataac agtaggcctt aaatactaaa atatttctat aaattaa
//
LOCUS       DAVAAL010000145         4009 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVAAL010000145 DAVAAL010000000
VERSION     DAVAAL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.147"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..547
                     /locus_tag="VQM60_001860"
     CDS             <1..547
                     /locus_tag="VQM60_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET1081685.1"
                     /translation="DHISSSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKK
                     ELGIELPIDEAAFIALHFVNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSID
                     YYRFITHIKLFAHRLVENTTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYL
                     LTSSELVYLMAHIRRLTKNLD"
     gene            565..2403
                     /locus_tag="VQM60_001861"
     CDS             565..2403
                     /locus_tag="VQM60_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET1081686.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVNQGYKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            2416..3831
                     /locus_tag="VQM60_001862"
     CDS             2416..3831
                     /locus_tag="VQM60_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET1081687.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLLGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     EEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
BASE COUNT         1345 a          529 c          795 g         1340 t
ORIGIN      
        1 ggatcatatt tcttcgagca tagaacgtta taaagaagga gttattattt cgaatcccct
       61 acgctgggaa atctcgaaat attataaaga agaatttgaa cttgggaaaa gggctttaca
      121 aataataaaa aaagagttag gtattgaact tccaattgac gaagctgcat tcatagcgct
      181 acattttgtt aatgctaatt tagaaaataa ttttcaagag tcgtataaaa tcactgaaat
      241 aattatggga attgagaaaa tcattcaaga tttctattgt actgagttta accaagattc
      301 tattgattat tatagattca taactcatat aaaattattt gcccatcgct tggttgaaaa
      361 tacaacttat tgtgacgatg atgatgaaga cttgttagca ttaatgaaaa ataaatatcc
      421 tagagaatat gaatgtggtg aacaggtggc aatgtttata caaactgagt acaattattt
      481 gctgacttct agtgaattag tttatttgat ggctcatatt cgtcgcttaa caaaaaattt
      541 agattaatag aataggagaa taccatgagt tataaagata cggtacaaaa aatcctcgat
      601 gtaattggag gtgaaaaaaa tgtcaataga gttacccatt gtgtaacacg tttaagatta
      661 gaattaaaag atgaaaattt agtcaatgat gatgatgtga agaagatacc aggtgtaata
      721 ggtattatga aaaaaaatgg acaatatcaa attatacttg gtaatgatgt agctaattat
      781 tataaagaat tcgttaaact tggcaatttt gaatccgatt cagttaatca agggtacaaa
      841 gggaatattt tagaaagaat cattgagtat atcgctggtt ccatgactcc aatcattcca
      901 gcaatgttag ggggaggtat gctgaaagtc ttggtaatta ttttaccaat gcttggtata
      961 ttgcaatcag attctcagac tattgctttt ttgacatttt ttggggatgc tccatattat
     1021 ttcttaccgc tgttattagc ttattctgca tcacaaaaat taaaagtaac atctacatta
     1081 gctatgtctg tagcaggtgt acttctccat ccaaattttg ttcaaatggt gcaatcaggg
     1141 aatcctctta gtttatttgg tgcacctgtg acaccagcta gttatggttc atcagtcgtt
     1201 ccaattctta ttatggtttg gttgatgaaa tatattgaaa aaataattgc taaattaaca
     1261 ccagctatta ctaagagttt tttgcaacct acgctagtat tattagtatc aagctgtatt
     1321 gccttagttg tagtcggacc tattggagta attgttggtg aaggattatc aaatctagtt
     1381 gggcaaatgt atggtgtagc tggatggctt acattagcta ttcttggtgc tattatgcca
     1441 tttattgtta tgactggaat gcattgggct tttgcaccta tttttttggc ggcatctatt
     1501 gctactccag acgtattaat tcttccagca atgttagggt caaacttagc tcaaggggct
     1561 gcttcgatgg ctgttgcatt aaagagtaaa aataataata caaaacaaat tgcttttgca
     1621 gcaggtttct cagccttact tgcagggatt accgaacctg cattatatgg tgtgacttta
     1681 aaatataaaa aaccgcttta tgcagctatg attggtggtg gattagcggg attatttgca
     1741 ggtcttacta gtgttaaagc atatctattt gctgtcccat ctttgatagc gttgcctcaa
     1801 tttatttatt ctgatgtgcc atcaaatatt gtaaatgctt taattgtggc ggtcatttcg
     1861 gttgttatta cctttgtatt agcttatata tttggaatcg atgaagaaga gagttctagc
     1921 aatttagaag ttgaagctgg agtttcaaat aaaaaaatga tattttctcc tatatcagga
     1981 gaaatcattc cgttaagcga tgtccaggat aaaacatttt cagataaact aattggagac
     2041 ggagtagcga ttatcccaag tgaaggtaag gtttatgcac catttgatgg gaaaattaca
     2101 aatatttttc cgactaagca cgcaattgga ttgaagagtg atgagggtgt tgagttacta
     2161 attcatattg gattagatac tgttgagcta aaaggtcaag gttttattag tcatgtagaa
     2221 gaaggagaca gagttttcaa aaatcagttg atttttgaaa tggacttgaa tttaatcaag
     2281 actaaaggct acgaaacagt tacaccagta attgtaacga ataccaatga ttttctagat
     2341 gtattagtat tacctaataa tcagacaatc gagcattcta aggaattact ggtaatatta
     2401 taatggagga aagttatgac catttttcca gatgattttc tttggggtgg agcggttgca
     2461 gctaatcaag tagagggagc atataatgaa gatggtaagg gattatcagt tcaggatgtg
     2521 ttacccaaag gtggattagg agaagcaaca gaaaatccta cagaagataa cttaaaattg
     2581 ttaggtattg atttttatca taaatataag gaagatatat ccttgttttc tgaaatgggc
     2641 tttaatgttt ttcgtacttc tattgcatgg agtagaattt ttccaaaagg agatgaagaa
     2701 gaacctaacg aagctgggtt gaaatattat gatgaattat ttgatgaact acatgctcac
     2761 gggatagaac cacttgtaac tctttcacac tatgagactc cattatattt agcaagaaaa
     2821 tatcatggat ggattgatag gagaatgatt catttttatg aaaaatttgc tcgaacagtt
     2881 ttagaaaggt ataaagataa agttaaatat tggcttacat ttaacgaagt aaactctgtt
     2941 ttggaattac cgtttactag tggaggaata gatataccta aggagaatct ttcgaaacaa
     3001 gaattatatc aagctataca tcatgaatta gtcgcctcga gtttggttac aaaaattgct
     3061 cgtgagatta attcagagtt taaggtgggg tgtatggtat tagctatgcc agcttatcca
     3121 atgactccaa atccaaaaga tgtatgggcg actcatgagt atgagaatct aaattatcta
     3181 ttttcagatg tgcatgttag aggttattat ccgaattatg caaaaagata ttttaaggaa
     3241 catgacatta acatagagtt tgcagctgaa gatgcagagt tattaaaaaa ttatactgta
     3301 gattttttat cctttagcta ttacatgagt gtgactcaat ctgctcttcc aacacagtat
     3361 aattcaggag aagggaatat tattggtggc ttagtaaatc cttatttaga gtcttctgag
     3421 tggggatggc aaattgatcc aattggacta cgtataattt taaatagata ttatgaccgt
     3481 tatcaaattc cattatttat agtggaaaac ggattaggtg ctaaagatca actaataaaa
     3541 gatgaattta ataacttaac agtccaagat gattatagaa ttcagtatat ggaagaacat
     3601 ttattgcaag tagctgaagc tctacaagat ggtgtggaaa ttatgggcta tacatcttgg
     3661 ggatgtattg actgcgtttc tatgtccact gcacaacttt ctaaaaggta cggtctcatt
     3721 tatgttgatc gaaatgatga tggaaatggc acattcaatc gatataagaa aatgtccttt
     3781 acttggtata aaggagtgat tgaatcaaat ggagaatcct tattcaaata gtttgtttat
     3841 tgatgattag atattatcac ggttaaggca acatctcatt aaatatgaaa taaagaggtt
     3901 tggaagacat caaattctaa acctctttat tttatagggg gattaattaa tttaattttg
     3961 ttacagagag ccgattataa atagtaaact taggatagat tggttatat
//
LOCUS       DAVAAL010000146         3948 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVAAL010000146 DAVAAL010000000
VERSION     DAVAAL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.148"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            60..947
                     /locus_tag="VQM60_001863"
     CDS             60..947
                     /locus_tag="VQM60_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HET1081688.1"
                     /translation="MLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIAST
                     LYFYLSPISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATAL
                     MPPLCTVGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDS
                     KMRRILLLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHT
                     LKLTISGNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKD
                     KEAKEKEKANKFYTESEEQ"
     gene            1114..3201
                     /gene="topA"
                     /locus_tag="VQM60_001864"
     CDS             1114..3201
                     /gene="topA"
                     /locus_tag="VQM60_001864"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET1081689.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEVASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3307..3666
                     /locus_tag="VQM60_001865"
     CDS             3307..3666
                     /locus_tag="VQM60_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET1081690.1"
                     /translation="MRLIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            3759..>3948
                     /locus_tag="VQM60_001866"
     CDS             3759..>3948
                     /locus_tag="VQM60_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET1081691.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPR"
BASE COUNT         1205 a          716 c          846 g         1181 t
ORIGIN      
        1 ccatgcttat cgcctctatc ggattaaata tggattcgac tcccgtgatt attggagcca
       61 tgttaatctc tcctttgatg acacctattc tgggagtggg gctctctcta gctatatttg
      121 attttaaatt gttaagaaaa tcttttaaaa tattagctat tcaaattctt gccagtttaa
      181 tagcttcaac actttatttt tatctttctc ccatttcgta tgctagttcg gaaattgttg
      241 ctagaacctc tccgactatt tgggatgttc tcattgcttt tgtaggaggg atagcaggta
      301 ttattggtgc taggaaaaaa gagaccaata atattgttcc tggtgttgct attgcaaccg
      361 ccttgatgcc tcctctttgt acagtaggtt atgctattgc ttctgctaat ctaaaattta
      421 tcataggctc ctcttaccta ttcctcatca attgtagctt tattgtcatt gcgacttata
      481 taggtgttag gttgatgatg gttaagaaac attattttaa agataatgaa gaagactcta
      541 aaatgcgtag gattttgctt ctagttgctg ttttgctgat gattccgagt ttcatctctg
      601 caacgacttt agtgagagaa acgttgaaaa aagagtccct taagaaattt atatcagagc
      661 agtttcaggg gcataatatt ttgaaaaaaa cctattctaa aaagactcat accctaaagc
      721 taaccatttc aggaaattat ttgacagaag aagaactcga tatgatttca agtaagagag
      781 gtgactatgg tttaagtgat gtttctgttc aagtttcaca attgtctgat tcagaacaac
      841 ttagtaagga agaactggtg gagtatttct tccagtatat caaggataag gaagcaaaag
      901 aaaaggaaaa agctaataag ttttatacag agtctgagga gcaataattt cttgagaata
      961 gctggttttt ctcgtgagtc ttctatgtat atcaaaggaa gactgaggtt ttaagtataa
     1021 aacttttctt ctattatagt agctaagtct tgacagggtt caaaaaatgg tttacacttt
     1081 ataaagttta ttactttgaa aaggtgtgat actgtggcta cggcaacaaa aaagaaaaaa
     1141 tcaacagtta aaaaaaatct agtcatcgtg gagtcgcctg ctaaggccaa gacgattgaa
     1201 aaatatctag gcagaaacta caaggtttta gccagtgtcg ggcatatccg tgatttgaag
     1261 aaatccagta tgtccgtcga tattgaaaat aattatgaac cgcaatatat taatatccga
     1321 ggaaaaggcc ctcttatcaa tgacttgaaa aaagaagcta aaaaagctaa taaagttttt
     1381 ctcgcgagtg acccggaccg tgaaggagaa gcgatttctt ggcatttggc ccatattctc
     1441 aacttggatg aaaatgatgc caaccgtgtg gtcttcaatg aaatcaccaa ggatgcagtc
     1501 aaaaatgctt ttaaagaacc tcgtaagatc gatatggact tggtcgatgc ccaacaagct
     1561 cgtcggatct tggaccgctt ggtagggtat tcgatttcgc ctattttgtg gaagaaggtc
     1621 aagaagggct tgtcagcagg tcgcgttcag tccattgccc ttaaactcat cattgaccgt
     1681 gaaaatgaaa tcaatgcctt ccagccagaa gaatactgga cagttgatgc tgtctttaaa
     1741 aagggaacca aacaatttca tgcttccttc tatggagtag atggtaaaaa gatgaaactg
     1801 accagcaatg acgaagtcaa ggaagtcttg tctcgtctga cgagtaaaga cttttcagta
     1861 gatcaggtgg ataagaaaga gcgcaagcgc aatgctcctt taccctatac cacttcatct
     1921 atgcagatgg atgctgccaa taaaatcaat ttccgtactc gaaaaaccat gatggttgcc
     1981 caacagctct atgaaggaat taatatcggt tctggtgttc aaggtttgat tacctatatg
     2041 cgtaccgatt cgactcgtat cagtcctgta gcgcaaaatg aggtggcaag cttcattacg
     2101 gatcgttttg gtagtaagta ttctaagcac ggtagcaagg tcaaaaacgc atcaggtgct
     2161 caggatgccc atgaggctat tcgtccgtca agtgtcttta atacaccaga aagcatcgct
     2221 aagtatctgg acaaggatca gctcaagcta tataccctta tctggaatcg ttttgtggct
     2281 agccagatga cagcggccgt ttttgatacc atggctgtta aattgtctca aaaaggggtt
     2341 caatttgctg ccaatggtag tcaggttaag tttgatggtt atcttgccat ttataatgat
     2401 tctgacaaga ataagatgtt accggacatg gttgttggag atgtggtcaa acaggtcaat
     2461 agcaaaccag agcaacattt cacccaaccg cctgcccgtt attctgaagc aacactgatt
     2521 aaaaccttag aggaaaatgg ggttggacgt ccatcaacct acgcgccaac cattgaaacc
     2581 attcagaaac gttattatgt tcgcctggca gccaaacgtt ttgaaccgac agagttggga
     2641 gaaattgtca ataagctcat cgttgaatat ttcccagata tcgtaaacgt gaccttcaca
     2701 gctgaaatgg aaggtaaact ggatgatgtc gaagttggaa aagagcagtg gcgacgggtc
     2761 attgatgcct tttacaaacc attctctaaa gaagttgcca aggctgaaga agaaatggaa
     2821 aaaatccaga ttaaggatga accagctgga tttgactgtg aagtgtgtgg cagtccaatg
     2881 gtcattaaac ttggtcgttt tggtaaattc tacgcttgta gcaatttccc agattgccgt
     2941 catacccaag caatcgtgaa agagattggt gttgagtgtc caagctgtca tcagggacaa
     3001 attattgagc gaaaaaccaa gcgtaatcgc ctcttctatg gttgcaatcg ctatccagaa
     3061 tgtgaattta cctcttggga caagcctgtt ggtcgtgact gtccaaaatg tggcaacttc
     3121 ctcatggaga aaaaagtccg tggtggtggc aagcaggttg tttgtagcaa aggcgactac
     3181 gaggaagaaa agattaaata agaggagggg cccgaaagta aatttcaggc tccttttggt
     3241 tagaacttga caaaatacat cattttatgc gaaactagaa gaagattatt ttaactagga
     3301 gaagttatgc gtcttatcta tctgataatt ggttttttat cactgacctt ggctattgtt
     3361 ggggttgttt tacccttgtt gccgacaacg cctttccttt tgttgtctat tgcttgtttc
     3421 tccagaagtt ccaagcgttt cgaagactgg ctttatcata ccaagctcta tcaaacatat
     3481 gtagctgatt ttcgcgagac taagtctatt acgcgtgaac gaaagaaaaa aatcatcgtc
     3541 tctatctacg tcttgatggg aatttctatt tattttgcac ctcttttacc agtcaaaatc
     3601 ggtctgggtg ctttgaccat ctttatcact tattatcttt tcaaggtcat tccagacaaa
     3661 gaatagttaa aacagtagtt atttgccttg ataaaattga aagcatattc ataacaatat
     3721 gatataataa atttgaagta atattcaagg agaatcaaat gatttacgaa ttttgtgctg
     3781 aaaatgtgac tttacttgaa aaagcgatgc aggctggagc tcgtcggatt gaactctgtg
     3841 ataatctagc agttggtggg acaacaccca gctatggagt gactaaggca gcggttgaac
     3901 tggcagctaa ctacgataca accatcatga ccatgattcg gccacgtg
//
LOCUS       DAVAAL010000147         3897 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVAAL010000147 DAVAAL010000000
VERSION     DAVAAL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.149"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..813)
                     /gene="lytB"
                     /locus_tag="VQM60_001867"
     CDS             complement(<1..813)
                     /gene="lytB"
                     /locus_tag="VQM60_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HET1081692.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKE"
     gene            complement(855..1793)
                     /locus_tag="VQM60_001868"
     CDS             complement(855..1793)
                     /locus_tag="VQM60_001868"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET1081693.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            complement(1804..2604)
                     /locus_tag="VQM60_001869"
     CDS             complement(1804..2604)
                     /locus_tag="VQM60_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET1081694.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            complement(2854..3234)
                     /locus_tag="VQM60_001870"
     CDS             complement(2854..3234)
                     /locus_tag="VQM60_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET1081695.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            complement(3292..3651)
                     /gene="rplT"
                     /locus_tag="VQM60_001871"
     CDS             complement(3292..3651)
                     /gene="rplT"
                     /locus_tag="VQM60_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET1081696.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            complement(3703..>3897)
                     /gene="rpmI"
                     /locus_tag="VQM60_001872"
     CDS             complement(3703..>3897)
                     /gene="rpmI"
                     /locus_tag="VQM60_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET1081697.1"
                     /translation="KQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHLRK
                     ASMVHSGDYKRIKAMLTRLK"
BASE COUNT         1124 a          899 c          695 g         1179 t
ORIGIN      
        1 ttctttttca gctattttcc catcaaattt gagataaaac caagattcct tatcccaaat
       61 ccattcattg gctgccatgt agccaccgga ttttagataa taatagtgac cattctcgaa
      121 aatccattct ttatcagcat agtttccatt ttcttttatg taaaaccaag attggtattg
      181 tttgtcaaaa agccaacctt gctgtacttt ggcaccacta gcattcacat aagcttgatt
      241 aatccactga cttgtcagta gataaccacc ggatttgaaa taatagtcct tccctttaat
      301 ttggagccat tctttctctg cgtgctgtcc atctgctttg atataaaacc aagcatcgta
      361 ttgagaatca tagacccagt cttctattac tttggcacct gttgcaccaa cataggaagt
      421 tcctacccaa gcatttcgtt tcatctttcc atcttggtca agataataaa aggctccctt
      481 gtcttctacc cattctgatt tggccatata gccaccagat ttgaggtaaa aatagtcgtc
      541 accttgcttt agccattcat tttcagcata gttagcatct gcttttatat agaaccaaga
      601 ttgataatga gtatcaaaaa cccactcatt cgctgcttga ctgccatctt ctttcagata
      661 ctgttttcct tgccaagtac catcactagc cattgcaccc tctggactag ctaagaaaaa
      721 tagcagagct aaaaaaataa atcttacttt cttcattaac cttcttcctc tgttcttatt
      781 tattttattg taccaaaatt ctcctaaatt catattacaa actaataaaa aatcaagaac
      841 agattgattg caatttacct cagagactct tttacttcct gacggagttc ttccagactg
      901 ctaataccgt atttatccat gacttttggt aaattttcga tgatgtcagg acaggcataa
      961 ggattggtaa agttagctgt tccaactccg atagcagatg ccccagccag atacatttct
     1021 agggcagctt cagccgaatc cactcccccc attccaatga taggcaggtc tgttgtttgg
     1081 gctacttggc ggatgagttt gagggctact ggaaagactg ctggacctga cattccacct
     1141 gttccattgg ccaagattgg ttttctggtt ttgaggtcaa agcgcattcc aaccagagta
     1201 ttgatcatgg tcaagccact tgctcccgca tcttctgcag cttttgcgac agtaacgata
     1261 tcggtcacac tcggggttaa tttgacataa actggcactt ctgaggcttc cacagctgct
     1321 ttcaccacat cataagccaa atctggatct tgaccaatca aaagtccatg attacagtgg
     1381 tcaacattgg gacaagaaat attgagctcg atagctttta cattagttgc cttggaaatc
     1441 ccatgagaaa cagctgcata ctcttgtttt gaaaaaccag ctacattggc aataatagga
     1501 agatttggat attctctttc cagccaaggt agcttttcag ccaaaacaac ctctaaacca
     1561 ggattttgca agccaattgc attgagcata ccagcaggcg tctctgccac tcttggagtt
     1621 ggattcccaa aacgtggttc aagggttgtc gccttgatca taatagaacc taaaaggtct
     1681 aaatcatagt acttggcata ctcttgtcca aagccaaaac agcctgatgc tggaataatc
     1741 ggatttttca aatccaaacc aggtagagac acttgtaatc gatttgtagt cataattttc
     1801 tccttataat acaactgttc ctgtgcggaa aacaggacca tcttcacaga cgcgttggct
     1861 gaccgtctcg ctttctggta cttttagaac acaggcatag caagctccca tcccacaagc
     1921 catacgagat tccagagata aataggctct tgggtgatca tcaaaggttt gattgatata
     1981 cttcatcatt cctggagccc cacacgagta aacagcatca aactgactat ctaaatcatt
     2041 gataacaacg gaaacatttc ccttgatgcc ataagaacca tcatctgtcg ttacaaagac
     2101 ctgaccatac tgagccaatt ccgttttcaa aataacagca tccttattag caaaaccgag
     2161 gactgtcact actttcactc cacgttcatg caattccttg gccacctcaa gcaagggtgg
     2221 aacaccaatc ccaccaccaa caaggagaac ctgattctgc tcatcaaggt cagacaagtc
     2281 aaaaccattt ccctgaggcc ccatcacatc aagagtgtct ccctgactta aggttgaaaa
     2341 aattgcagtc ccagctccct caatccgata aatgaggtga cactgcttgt ttgccttgtc
     2401 aatagacgaa attgaaatag gacgacgtaa gagatgggca tcgtccggta cacgcagatg
     2461 aagaaattgg cctgctcgca tggcttcaac catttctcct tctaggacta attcaaagat
     2521 tgctggcgcg atttcctctt gtgcaaccac cttcatggtt tccaaccgaa tgacacccaa
     2581 acgcttctta catgtgagat tcataacttt tcctccttaa attttaaggg gaccagataa
     2641 agaaaagacc ttgctagtgc aaggcctcag ttaaaatcgt acaacacaag agagcacact
     2701 caaaaggtct cctctagaaa attgcactat tcttttatct gacaccttgt gagtctctct
     2761 ggactcccct taaaggttga atttttatta gtatactctt tcagctaaaa aaagtcaagt
     2821 agaaaacgaa tattctactt aacttgcacg aaattatttt tcacgaatga cttcgacctt
     2881 atatccatca gggtctttaa caaagtaata gtttggtgta gttcctggta gaccatttgg
     2941 ctcagtcact tcatagcctt ttgcactgtg ttcttgatga agtgcctcaa gatcaggtgt
     3001 actgagggcg atatgggcaa acccatcacc aaccacatac ggaccgtgat cgtagttata
     3061 agtcaactcc aactcataat catcaccctc aagacctaga tagacaatcg tgaaggcatg
     3121 gtctggaaaa tctctgcgac gcaattcttt aaaaccaaaa gcatcttgat aaaatgcaat
     3181 tgatttttca agattttcta ctcgtaagca agtgtgtagc atttttgaag ccatatcttt
     3241 ctcctttatt tttaaaaaga ctggacaatc ctgttccagt ctcatcagtt gttatttacc
     3301 aagttttgct ttagctgcat ctgcaagagc tgtgaaagct actgcatcgt taacagccaa
     3361 gtcagcaagc attttacggt taacttcgat ctcagccaat ttcaaaccat gcatcaattg
     3421 tgagtatgaa agtccgttca tacgagccgc cgcattgata cgagtaatcc acaatttgcg
     3481 gaagtcacgt tttttctgac gacggtcacg gtatgcatag tagtaagagt tcattacttg
     3541 ttcttttgca gtacggaaca agatgtgttt agctccatag taaccttttg ctaatttaag
     3601 aatacgttta cgacgtttgc gtgatacaac gccaccttta acacgtgcca tgttttattt
     3661 cctccaaata ttttctagaa ttgtttactt acagtcaagc tattatttca agcgagtaag
     3721 cattgctttg atacgtttgt aatctcctga atgcaccata gatgctttac gaagatgacg
     3781 acgttgtttc ttagtttttc cgtggaaacg gtgagaagtg taagcacgga aacgtttaag
     3841 tccaccagaa cctgtacgtt tgaaacgttt agctgatgcg cggtgtgttt tttgttt
//
LOCUS       DAVAAL010000148         3800 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVAAL010000148 DAVAAL010000000
VERSION     DAVAAL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.150"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            31..342
                     /locus_tag="VQM60_001873"
     CDS             31..342
                     /locus_tag="VQM60_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081698.1"
                     /translation="MLSGLIFFLAQGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGV
                     LTIDFLFFVLLAFISSQRFSSLMSIITACHSTFYYMTADYLRENYPDFYDKHISLWEC
                     L"
     gene            361..546
                     /locus_tag="VQM60_001874"
     CDS             361..546
                     /locus_tag="VQM60_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081699.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(813..1256)
                     /locus_tag="VQM60_001875"
     CDS             complement(813..1256)
                     /locus_tag="VQM60_001875"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET1081700.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(1286..3508)
                     /locus_tag="VQM60_001876"
     CDS             complement(1286..3508)
                     /locus_tag="VQM60_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET1081701.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
BASE COUNT          993 a          912 c          709 g         1186 t
ORIGIN      
        1 attagactcg agacatcttt tgagtggctc ttgctctctg gtttaatttt cttccttgct
       61 caaggactcc tgctatttct cttgatcgtc cgactcaaac atcaattcgc tgagatttat
      121 cctcaaatca ataaaaagat tcgcttctac tatttagggg ttctcaccat tgattttcta
      181 ttttttgttc tcttagcctt cattagttct cagcgttttt catctcttat gtcaattatc
      241 actgcttgcc attctacttt ttattatatg acagctgact acctaagaga aaactatcca
      301 gacttttacg acaaacacat ctctttatgg gagtgtctct aaggaaaagg aggttttagc
      361 atgaaaaaaa tcatcttcat caaaaccatt caactccttg tcattgatgg aatcatgctg
      421 gcatttttga catttaaaag ggggcttact tgggactgga ttttgattta tagcggttgg
      481 ctcattttct ttcatcctgt gctattgacc tatctttcaa accaactttg tgaccacttt
      541 agttaactct attcccagat tagaccgaga ttctggcgtt ttgctttaca aattctccta
      601 tgggatagcc tgatgattct ctccttggtg tctttaagtg atattccact tttccttcag
      661 ggaactctcc tcatcctagg acacctcatc ccttcctatc gcatctgcca aagcctgaaa
      721 agagacttcc cccaagcata tcaagaaccg atttcttttt ggagtatttt atgatagatg
      781 agaaagacca agccgactgg gcttggtctt tcttatctct ttttagtatc taggataatg
      841 gtaacaggtc cgttattaac cagctcaacc tgcatatctg ctccaaagat acctgtctga
      901 acgggcactt cttgcgctaa tttttgattg aaagcatcat agaagtctga tgccatatca
      961 ggtttagctg cccctgtaaa ggctggacga ttgcctttct tagtatccgc aaagagggta
     1021 aactgagaaa tagagaggat ttctccttca atatctttga cagacaggtt catcttgcct
     1081 tctgcgtctg aaaaaatccg catattgacc agttttctca cagcatagtc caaatcttcc
     1141 tcttggtcct ctggtccaac accaaccagc aataaaagtc cctgattgat ttttccctga
     1201 atctggcctt ctatactcac ttgggctttt ttaacccgtt ggataatgat tttcataata
     1261 gcctttctag taagagctag gacaactagc cgttggtccg tttgacagag taaacttctg
     1321 gcacactctt aattttatcg acaaccgtgg tcagtgtaga gaggttggca ataccgaagg
     1381 acacatggat attagcaaac ttcatatcct tggttggttg ggcattgacc gttgaaatat
     1441 tcttggttgt atttgaaaga acttgcagta catcgttcaa cagtcctgta cggttgagac
     1501 cgtagatatc gatatgggcc atatactcct tatttgagct agagtactgg tcttcccatt
     1561 ccacatcaag gagacgttgc tcgtagtttt cttgggcacg cagattcata cagtccacac
     1621 ggtgaatagc cacaccacga cccttggtaa tgtagccaac aatatcgtca ccaggcacgg
     1681 ggttacaaca cttagcaatc cgcactagga gaccagaagc accttcaata accactcccc
     1741 cctcatgctt gaccttgaga gtttctttat tttcaacctt gacctcgcca cctttgacaa
     1801 gctcctctgc ctcagccttg gccttggcac gctcttcctc acggcgttcc ttttcagtca
     1861 gacggttaaa gacggtaatc gcaccgattt ccccaaaacc aatggccgca aagagggagt
     1921 cttctgtctt gtaactggtc ttttgcagaa cttgatccat gtggcgcttg tccataaatt
     1981 tatttgccac atagccattt tcttggaact gagccatcag catctcacga cccttgttga
     2041 cagacaattc cttatcttgg tttttaaaga actggcgaat cttattgcgc gccttgctag
     2101 tcttgaccat attgagccag tcacggctag gtccaaagga gttcgggttg gcgataattt
     2161 caacctgatc ccctgtcttt aacttggttg tcagtggaac catgcggcca ttgaccttgg
     2221 caccagttgc tttttcaccg accttggtat ggatttcgta ggcaaaatca atcggtcctg
     2281 aatctttggg aagggaacgg acagctccat ctggggtaaa aacgtaaatc tcctcagcca
     2341 gatagttttc cttaacagag tccacaaatt ccttagcatc atcagcctgg tcttggagct
     2401 ccatcatctc cttgatccag ttcattccaa tagctgattc cttgctgtta acttgcccct
     2461 ttataccttt cttataagcc cagtgagccg caaccccgta ctcagccacc tcgtgcattt
     2521 ccttggttcg aatctggaat tcaatcggcc cttttggccc ataaacagtc gtatggatag
     2581 actgataacc attggccttg cggttggcaa tatagtcttt gaagcgacct ggcatcggtt
     2641 tccaaaattc atgcacgtaa ccaagcatgg cataaacatc actttgggta tctaaaatac
     2701 aacgaatagc aatcagatca tagatttcct caaaccgttt tctcttgtcc tgcattttgc
     2761 ggaaaattga gtaaatatgc ttgggacgac cataaatctt ccctttcaag tgacgttctg
     2821 tcgtatactc ctctaatttt gtgactacct catccaccaa ggcctcacgc tccctgcgct
     2881 tttccttcat catatgggta atcttgtaaa actccgttgg attgagataa cggaaagaca
     2941 agtcttctaa ttcccatttg acactggaaa tccccaaacg atgggcaagt ggggcataga
     3001 tttccatggt ttctttggaa atacgctcct gcttgtcttt tcgaagatgt ttcagggtcc
     3061 gcatattgtg caagcggtca gacagtttga ccaaaataac gcggatgtcc tcagacatgg
     3121 ccatgagcat cttgcgatga ttttccgcta attgctcctc gattgatttg tactcgacct
     3181 tgccaagctt ggtaactccg tcaacaatca cccgcacatc aggaccaaac tctctttcca
     3241 aatcgtccaa ggtcgcatct gtatcttcca ccacatcatg caagaatcca caagctactg
     3301 ttacagcatc cagctttagc ttagctaaaa tacctgccac ttggataggg tgaatgatat
     3361 aaggctcgcc tgatttgcga tattgaccac tgtggcattc aacagcatag accaaggcct
     3421 tatggacaaa atgaacatcc tcttccgtta aatattcttt ggttaaagcg acaacttctt
     3481 cgcctgttaa attcacttct ttcggcatct ctactctcca attcttccta ccattttatc
     3541 acttttttaa gaatatgaaa actagattgg aacagaataa gaaaaaaata attcaaaatt
     3601 gcttgataat tctgaattat cggcccataa tatactacga agttagattt taaacttagg
     3661 tgatagaagg agagatagaa gaacggaaac catattgtaa cccaaagact ttctgatttc
     3721 cccaattcca ttgaagatac gaaagataaa cggtggaact cgtatcacat acactggtac
     3781 cttgactgga ttttggaatt
//
LOCUS       DAVAAL010000149         3745 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVAAL010000149 DAVAAL010000000
VERSION     DAVAAL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.151"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            87..3437
                     /locus_tag="VQM60_001877"
     CDS             87..3437
                     /locus_tag="VQM60_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET1081702.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIEQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDNSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLEQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
BASE COUNT         1202 a          592 c          784 g         1167 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgaaaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tggagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttacatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgaattt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gaacaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctc ttgagaaaca tatcggctat cagccaattt
      961 gctttattac aaatgggttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cgcttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggcggggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcct
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcgt atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt ttactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagaca
     1801 atagctgtga aaaagatatt gatgggagtg tatttaattc ctttattttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgatca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgctgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg agcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccacagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagtt ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctttggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagctgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcagg
//
LOCUS       DAVAAL010000150         3736 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVAAL010000150 DAVAAL010000000
VERSION     DAVAAL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.152"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            262..2190
                     /gene="glgB"
                     /locus_tag="VQM60_001878"
     CDS             262..2190
                     /gene="glgB"
                     /locus_tag="VQM60_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET1081703.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            2180..3322
                     /locus_tag="VQM60_001879"
     CDS             2180..3322
                     /locus_tag="VQM60_001879"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET1081704.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            3312..>3736
                     /locus_tag="VQM60_001880"
     CDS             3312..>3736
                     /locus_tag="VQM60_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET1081705.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHST"
BASE COUNT         1131 a          650 c          840 g         1115 t
ORIGIN      
        1 ctttgatttc taagacattg ttagaaatta gattaaattc cttaatcgat ttgcgcatgt
       61 tttatttcat ttcactatag tagaaaaaga aaggcgtttt cgcaattttt ttgaaaaaaa
      121 tactgattta caattgaacg agtaagtgaa cttgttgtag attttcaaaa taattaaaga
      181 agtatagtaa tttacttgaa tcttctctaa aaaggtagta taataaaaac atagaaaacg
      241 cttacaggag aaggggagta tatggataat agagaagcat taaaaacctt tatgacgggt
      301 gaaaattttt atctccaaca ttatctagga gcacataggg aagaactaaa tggagagcat
      361 ggctatacct tccgtgtttg ggcacctaat gctcaggctg ttcacttggt tggtgatttt
      421 accaactgga ttgaaaatca gattccaatg gtaagaaatg actttggggt ctgggaagtc
      481 tttaccaata tggctcaaga agggcatatt tacaaatatc atgtcacacg tcaaaatggt
      541 catcaactga tgaagattga cccttttgcc gtcaggtatg aggctcgtcc aggaacaggg
      601 gcaatcttaa cagagcttcc tgagaagaaa tggaaggatg gactttggct ggcacgaaga
      661 aaacgttggg gctttgaaga gcgtcctgtc aatatttatg aagttcacgc tggatcatgg
      721 aaaagaaatt ctgatggcag tccttatagt tttgcccagc tcaaggatga actcattcct
      781 tatctcgttg aaatgaacta tactcatatt gagtttatgc ccttgatgtc ccatcctttg
      841 ggcttgagtt gggggtatca gcttatgggt tacttcgctt tagagcatgc ttatggccga
      901 ccagaggagt ttcaagattt tgtcgaggag tgtcataccc ataatattgg ggttattgtg
      961 gactgggtac caggtcactt taccatcaac gatgatgcct tagcctatta tgatgggaca
     1021 ccgacttttg aataccaaga ccataataag gctcataacc atggttgggg tgcccttaat
     1081 tttgaccttg gaaaaaatga agtccagtcc ttcttaattt cttgcattaa gcattggatt
     1141 gatgtctatc atttggatgg tattcgtgtg gatgctgtta gcaacatgct ctatttggac
     1201 tatgatgatg ctccatggac acctaataaa gatggcggaa atctcaacta tgaaggttat
     1261 tatttccttc agcgcttgaa tgaggttatt aagttagaat atccagatgt gatgatgatt
     1321 gcagaagaaa gttcgtctgc gaccaagatt acgggaatga aagagattgg tggtctagga
     1381 tttgactaca aatggaacat gggctggatg aatgatatcc tccgtttcta cgaagaagat
     1441 ccgatctatc gtaaatatga ctttaacctg gtgactttca gctttatgta tgttttcaag
     1501 gagaattatc tcttgccatt ctcgcacgat gaagtggttc atggcaagaa gagtatgatg
     1561 cataagatgt ggggagatcg ttacaatcaa tttgcaggct tgcgcaatct ctatacgtac
     1621 caaatttgtc accctggtaa gaaattgctc ttcatgggta gcgaatacgg tcaattccta
     1681 gaatggaaat ctgaagaaca gttggaatgg tctaacctag aagacccaat gaatgctaag
     1741 atgaagtatt tcgcttctca gctaaaccag ttttacaaag atcatcgctg tctgtgggaa
     1801 attgatacca gctatgatgg tattgaaatc attgatgcgg ataatcgaga ccagagtgtt
     1861 ctttccttta ttcgtaaggg taaaaaggga gaaatgttag tctgtatctt taatatggta
     1921 cctgttgagc ggaaagattt tacaatcgga ctacccgttg caggaattta cgaagaagta
     1981 tggaatactg agttggaaga gtggggaggc gtttggaaag aacataatca aactgttcaa
     2041 acgcaagaag gactatggaa agattatgag cagaccttaa cctttaccct accggctatg
     2101 ggagcaagtg tatggaaaat caaacgtcgc ttgaaatcta ctaaaaccgt cacaaataaa
     2161 aaccaaaaag gagtagaaaa tgaaaaatga aatgttagct ttgattcttg ctggtgggca
     2221 aggaactcgt ctcggtaaac tcactcaaag catcgcaaaa ccagctgtgc aatttggtgg
     2281 gcgctaccgt atcattgact ttgccctatc aaactgtgcc aactcaggga ttcacaatgt
     2341 tggggtcgtt acacagtatc aaccacttgc tctcaacaac catattggga atggttcaag
     2401 ctggggacta gacggtatta attcaggtgt ctctattctt caaccttatt ctgcaagtga
     2461 aggaaatcgt tggtttgagg ggactagtca cgctatttac caaaatatcg actatatcga
     2521 cagtgtcaat cctgagtatg tcttgatttt gtctggggat cacatctaca aaatggacta
     2581 tgatgatatg ctccagtctc ataaggataa taatgccagc ttgacagtag cagttttaga
     2641 cgtccctctt aaagaagcaa gccgttttgg tatcatgaac acagatgcta acaatcgtat
     2701 tgttgaattt gaagaaaaac cagctcaacc taaatctaca aaagcttcta tgggaatcta
     2761 catttttgat tggcaacgcc ttcgtaatat gttagtcgct gctgaaaaga gcaaggttgg
     2821 catgtcagac tttggtaaaa atgtcattcc aaattacctt gagtcaggtg aaagtgttta
     2881 tgcttacgaa tttagtggtt attggaaaga tgttggtact attgagtcac tttgggaagc
     2941 gaacatggag tatatttctc cagaaaatgc cttggatagt cgtaaccgtc aatggaagat
     3001 ttactcaaga aacttgattt caccaccaaa cttcctcggg gcaaatgctc atgtggaaga
     3061 ctcattagtt gtagacggat gtttcgttga tggaactgtt aaacattcta tcctttcaac
     3121 aggcgcgcaa gttcgcgaag gagcggaagt ccttgattca gttatcatga gtggagctat
     3181 cattggtcaa ggagctaaga ttaaacgtgc cattattggt gaaggtgcga ttatttctga
     3241 cggtgtcgaa attgatggaa cagatgaagt acaagttgta ggatataatg aagtagtggg
     3301 ggtagcaaca gatgaagatt gataaatatt ctgccatttt aggaaataca gttggttttc
     3361 acaatatgtc gacattgacg gaccaccgtc cagtagcaag tttgccattt ggtgggaaat
     3421 atcgtttgat tgacttccca ctttcaagcc ttgctaatgc aggtgttcgt agtgtctttg
     3481 gtattttcca gcaggataat atcagctcag tatttgacca tattcgttca ggacgcgagt
     3541 ggggcttatc aacccttctt agccattact atctaggaat ttacaatacc cgtgtagaaa
     3601 gtagtacagt tggaaaagaa tactaccaac agcttcttac ttatttgaaa cgttctggct
     3661 caaaccaaac ggttgccctc aactgcgatg ttcttattaa cattgatttg aaccaagttt
     3721 tccacctaca tagtac
//
LOCUS       DAVAAL010000151         3625 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVAAL010000151 DAVAAL010000000
VERSION     DAVAAL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.153"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(1..801)
                     /locus_tag="VQM60_001881"
     CDS             complement(1..801)
                     /locus_tag="VQM60_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET1081706.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            972..1577
                     /locus_tag="VQM60_001882"
     CDS             972..1577
                     /locus_tag="VQM60_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET1081707.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            1590..2990
                     /gene="pepV"
                     /locus_tag="VQM60_001883"
     CDS             1590..2990
                     /gene="pepV"
                     /locus_tag="VQM60_001883"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET1081708.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            3044..3622
                     /locus_tag="VQM60_001884"
     CDS             3044..3622
                     /locus_tag="VQM60_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET1081709.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
BASE COUNT         1073 a          741 c          806 g         1005 t
ORIGIN      
        1 ttattgtaac tgagattgat ctgggaggta agaaccacct agataggtat tgctgagttt
       61 ttcaagggtt ccgtcttgat agagttcttt gagcgcttta tcaaattgct ctttaaactc
      121 tttttggtcg cttgagaaaa tgatataatt gctggggcta tctgcagaag gtaaatcaac
      181 gactgagagg tctaaaccac ggtccttgat aatcttttga acggatacct tgtcaaaaac
      241 taggaaatca aactctccgt tagcaaggtc taggattcgt ttaccaatat cctcaccaga
      301 aaaattaatt gtagcgggat tatcagtgtg tttctgattc cagttattga tgaattgagc
      361 gttagaagtt ccggtatcct cttgtgttgt tttaccagcg atctggtcaa gagaagtcaa
      421 aggatttttc ttgttgctga caaggacgag gggattgttg gaaatcggaa gcgagtaaag
      481 gtatttttca gcacgctctt ttgtgtaact caaattattg gccgcagcct gatagtgacc
      541 agaatcaagt cctgggaaga tactttccca ggcggttctt tgaaattgaa tctcgtagtc
      601 gctgagtttt tcatctactg cctttagaac ttcgatatcg aagcctgtca gattgccttt
      661 atcttcgtag tcaaatggtg gcacgtcgcc agctgtagca aggacgattg tcttttgagc
      721 gctagtctct ttgggtgtag cttgattccc acaggcaacc aaaaatggta ggatagctag
      781 taataggcta aattttttca tactgtctcc attcaaatgt aaagtacttg ctagtttatc
      841 agaaactttc ctgataaacc aatatatatc ttttatgtct gtgatagaaa aacttaatca
      901 tgaaaaatcc ctgcaagctc tttcaaatta tggtagaatg gaagcagtag aattagaaaa
      961 ggaaatcgat tatgaaattt cttgaattaa ataaaaaacg tcatgcgacc aagcatttca
     1021 ctgataagcc tgttgatcca aaagatgtgc gtacggctat cgaaattgca accttggcgc
     1081 caagcgccca caacagccag ccttggaaat ttgtagttgt tcgtgagaaa aatgctgaac
     1141 tggcaaagtt ggcttatggt tcaaactttg agcaggtatc atcagcacct gtaaccattg
     1201 ccctatttac agatacagac cttgccaaac gcgctcgtaa gattgcccga gttggtggtg
     1261 ctaacaattt ctcagaagaa caacttcaat acttcatgaa aaatttgcca gctgaatttg
     1321 cacgctacaa tgaacaacaa gtcagcgact acctagctct caatgcaggt ttggttgcca
     1381 tgaacttggt tcttgcattg acagaccaag gaattggttc taacattatt cttggttttg
     1441 acaaatcaaa agttaatgaa gttttggaaa tcgaagaccg tttccgccca gaactcttga
     1501 tcacagtggg ttatacagac gaaaaattgg aaccaagcta ccgcttgcca gtagatgaaa
     1561 tcatcgagaa aagatagaaa gaagaaaaaa tgacagcaat tgattttaca gcagaagtag
     1621 aaaaacgcaa agaagacctc ttggctgact tgtttagcct tttggaaatc aattcagaac
     1681 gtgatgacag caaggctgat gcccagcatc catttgggcc tggtccagta aaagccttgg
     1741 agaaattcct tgaaatcgca gaccgcgatg gctacccaac taagaatgtt gataactatg
     1801 caggacattt tgagtttggt gatggagaag aagttctcgg aatctttgcc catatggatg
     1861 tggtgcctgc tggtagcggt tgggacacag acccttacac accaactatc aaagatggtc
     1921 gcctttatgc gcgcggggct tcggacgata agggtcctac aacagcttgt tactatggtt
     1981 tgaaaatcat caaagaattg ggtcttccaa cttctaagaa agttcgcttc atcgttggaa
     2041 cagacgaaga atcaggctgg gcagacatgg actactactt tgagcacgta ggacttgcca
     2101 aaccagattt cggtttctca ccagatgctg aatttccaat catcaatggt gaaaaaggaa
     2161 atatcacgga atacctccac tttgcaggag aaaatacagg tgttgcccgt cttcacagct
     2221 ttacaggtgg tttacgtgaa aatatggtac cagaatcagc aacagcagtc gtttcaggtg
     2281 acttggctga cttgcaagct aaactagatg cctttgttgc agaacacaaa cttagaggag
     2341 aactccaaga agaagctggc aaatacaagg tgacgatcat tggtaaatca gcccacggtg
     2401 ctatgcctgc ttcaggtgtc aatggcgcaa cttaccttgc cctcttcctc agtcagtttg
     2461 gctttgctgg tccagccaaa gactaccttg acatcgcagg taaaattctc ttgaacgatc
     2521 atgagggtga aaatcttaag attgctcatg tggatgaaaa gatgggtgct ctttctatga
     2581 atgccggcgt cttccgcttc gatgaaacaa gtgctgataa taccattgcc ctcaacatcc
     2641 gctatccaaa aggaacaagt ccagaacaaa tcaagtcaat ccttgaaaac ttgccagttg
     2701 tttctgttag cctgtctgaa cacggtcaca cgcctcacta tgtgccaatg gaagatccac
     2761 ttgtgcaaac cttgttgaat atctatgaaa aacaaactgg ctttaaaggt catgaacaag
     2821 tcatcggtgg tggaaccttt ggtcgcttgt tagagcgcgg agttgcctat ggtgctatgt
     2881 tcccagactc aattgatacc atgcaccaag ccaatgaatt tatcgccttg gacgatctct
     2941 tccgagcagc agcaatctat gccgaagcta tttacgaatt gatcaaataa aacgatagaa
     3001 gtctgagatc ttatgcttgg acttcttttt ggagggaaag tagatgtctc aaatcgaaag
     3061 aatcaaacag gctatcatgg cggattcaca gaatgccagc tatacagagc gtggcattga
     3121 gcctctcttt gcagcgccaa aaactgctcg catcaatatc atcggtcagg ctccgggact
     3181 taaaactcaa gaagcaggcc tttactggaa agataaaagt ggtgaccgct tgcgggactg
     3241 gctaggtgtg gatgaagata ccttttacaa ttcaggttat tttgctgttt tgcctatgga
     3301 tttctacttt ccaggacatg gcaagtcggg tgatcttccg cctcgtacag gttttgcaga
     3361 aaaatggcat ccgcagctct tacaggaatt gcctgatatt cagttaaccc tcttgattgg
     3421 gcaatatgcc caagcctact atttacagga gaaaatcagt gggaaggtaa cggagagggt
     3481 gaaacactat aaagactatc tgccagccta ttttccgcta gttcacccat caccacgaaa
     3541 tcaaatctgg atggccaaaa atccttggtt tgaggcagaa gtagtgccag atttgaaaaa
     3601 aagaattaaa accattttat agtca
//
LOCUS       DAVAAL010000152         3484 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVAAL010000152 DAVAAL010000000
VERSION     DAVAAL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.154"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            13..1024
                     /locus_tag="VQM60_001885"
                     /pseudo
     CDS             13..1024
                     /locus_tag="VQM60_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(1295..1516)
                     /locus_tag="VQM60_001886"
     CDS             complement(1295..1516)
                     /locus_tag="VQM60_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081710.1"
                     /translation="MDLFQELIYLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            1481..2110
                     /locus_tag="VQM60_001887"
     CDS             1481..2110
                     /locus_tag="VQM60_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081711.1"
                     /translation="MSKVNQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIEIASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            complement(2146..3369)
                     /gene="pepT"
                     /locus_tag="VQM60_001888"
     CDS             complement(2146..3369)
                     /gene="pepT"
                     /locus_tag="VQM60_001888"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET1081712.1"
                     /translation="MTYPNLLDHFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQMLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
BASE COUNT         1177 a          655 c          667 g          985 t
ORIGIN      
        1 aatgaggtaa ttatgaaatt taaaaagaaa tatatagcag ctggatctgt tgttatcctt
       61 tccttaagtc tgtgtgttta tgctctgaac caacatagct aacaggccaa tacagataaa
      121 aatcgtgttt catatgtaaa cagtaataaa gacactaaga agactgaaaa tttgactcca
      181 gactaggtta gtcaaaaaga aggaattcaa gcaaaacaaa ttgttattaa gattacagat
      241 caaggttatg taacttctca tggcgatcat tatcactact ataatggtaa agttcctttt
      301 gatgcgctct ttagtgaaga gctattaatg aaagccccca actatcagcc gaaagatcaa
      361 gatatcgtca gttaggttaa gggaggatat attattaagg tagatggaaa gtattatgtt
      421 taccttaaag atcaagctca tgcagaaaat gtacgaacaa aagatgaaat caatcgccaa
      481 aaacaagaac atggtaaaga cgataaagga gctagtgcag aagtatctgt agcaaaatta
      541 cagggacgtt atactaccga tgatggttat atatagtgag ttgaatttag aatagcacat
      601 catagtttct aaatcattgt tagaaattac tttaaaatcc ccttatcaat ttgctcatat
      661 tctatttcaa tctactatag taccacatgg tggtcactat catttcattc ctgagagtga
      721 cttgtcggca ggtgaattag cagccacata tgtctttaac cctaatgata ttgtcagaga
      781 tacaggtgat gcttatatag taagacatgg ggatcactat cactatattc ctaagtcatc
      841 tcttaacaat cccccatcac actctaacac ggaagaggta ggaagttctt ctagttctgt
      901 attaagtaat ccgtcactac atgtccatca tgaggaagaa gatggtcatg gttttgatgc
      961 caatcgaatt ataagtgaag attcagaagg ttttgtgatt cctcatggcg accacaatca
     1021 ctacatcaag gttcaaacta agggatatga agcggctttg aaaaacaaga ttccatccct
     1081 gcaatccaac tatcaacctg gaacttttga tgagaaggca gtcttggcaa aagtagatca
     1141 acttctagct gatagcagaa gcatctacaa agacagattg agttagcttt aggtcagttt
     1201 actgaaagtt tgaaaaaaat aaaagttagt tgattgatta cttctctaaa ttttgggcac
     1261 actaaaaaga ggtcttgaca cctaaacttt aaaattaaag catattgaat ccgtgctgct
     1321 gtgcgaagcg aatagcgtct tggacgaaca ttgtcttatc tggatttctt atttctgtaa
     1381 ggccttgtaa tcttggatta taactctcct gttttaggag tacatggtag atggcaacca
     1441 ataatctgcg acagatagca aggaagaagt agaaagtctt ttgagcaagg taaatcaact
     1501 cttggaacaa atccatgaag aaggaatcag acaatccttg gcagaagaag tagaaaatct
     1561 aaaagctgcc acaaacaagg ctgatgcaga cttggatgaa gtaaacagtc aggtaaaaga
     1621 tgtcttgact cgtatcgcta gcgcccttca acaagaaaag gaaaatgctg agcaagattc
     1681 tcagacactt gtactctatc aaaaactcta cgatattctc atgtcgcttc actcttattt
     1741 agaaagtaat aatggatctg atgcggactt tgataaggtg gatgcattat tagatcaact
     1801 ttctgctaag agtaaagaca aaacagcttt acttgaatcg acaaaagcta ttctagtttt
     1861 gaatcatcaa atccagtcaa aagctagcgc aagtgaagaa acaagtccag caagaaatgc
     1921 tgaagcaaat agtgataaca ccagtgctga gaaccaacca aacgcaactg cagaatctaa
     1981 cattgaaatt gctagcgacg aaaacaaacc aagcaataca agagattcta aacctgctga
     2041 atcaacttca gaaaatgaaa caacagaatc ttctacaact actggaaatc aagaaaaacc
     2101 agtagaataa aaagaaaggc gaagtagctg agctacctcg tctttttagc ctttataagc
     2161 tacgatgcca atgatggtat caactgcacg ttccatagtc tgaaggctaa cgtattcaaa
     2221 acgtccgtgc atattttcgc cacctgcaaa gatattcgga gttgggattc ccataaagga
     2281 aatcttagag ccgtctgttc caccccggat tggttcgata ataggcgtga tacctagatc
     2341 ttccataacg gctttagcaa tggtaattgg agtcatatct ttttcaatga cttctttcat
     2401 attgtagtac tggtctgtca agttgagagt gacacggtag ctcccaagtt ctgcattcat
     2461 cttatcagcg atagattgca tggatgcttt acgcgcttca aaggcatctt tttcaaaatc
     2521 acgaatgatg tagcttgcac gcacctcctc aacactacct gtcacatcca ttagatggta
     2581 aaaaccttgg taaccttcag ttaactcagg tcggtcattt tctggaagtt gattatgaaa
     2641 atcaattgct agctgaaggg cattgaccat ctgctctttg gcagtaccag ggtggacatt
     2701 acggccttgg aaatgcaatt cagcaccagc ggctgagaaa gtctcgtact gaagttcacc
     2761 tagtggacca ccatcaacag tgtaggcaaa atccacatca aaatcttctg catcaaattt
     2821 attggcacca acaccgattt cttcatctgg accaaaacca acacgaatct cacagtgctt
     2881 aatttcagga tgagcagtta gatattcaat ggctgtcata atttcagcaa ttcctgactt
     2941 gtcatcagca cctagcaagc ttgttccatc tgttgtgatg agcatttgtc ctggatattt
     3001 ttcaagactc ttgaagtcag ctggatcgag tttgaaacca gaattcccta gttcaatcac
     3061 accaccatcg tagttttcaa ttacctgtgg attgactcct tcagcattaa aatcagcagt
     3121 atccatgtgc gatataaaac caatcttacg tgttaaagac ggatcgttgg ctggcaaggt
     3181 tccaatagca aaaccattcg gtagatagta aacattttgc agtccaacac gtttcatttc
     3241 aggaattagg acatttgttg cgaagtcaac ctgactctgt gtacttggag tagtagtaga
     3301 gtgttcatca gagcgcgtgt tgaccttaac gtaggttaag aagtggtcca agagatttgg
     3361 ataagtcata aaaaaactcc ttttttatca taaaatagaa tacaaaactt cggaaaaata
     3421 tgatttgaga aattttatga attttgaaaa aatataataa ttcaaattca gaagactttc
     3481 aaaa
//
LOCUS       DAVAAL010000153         3428 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVAAL010000153 DAVAAL010000000
VERSION     DAVAAL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.155"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..208)
                     /locus_tag="VQM60_001889"
     CDS             complement(<1..208)
                     /locus_tag="VQM60_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET1081713.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFK"
     gene            306..626
                     /locus_tag="VQM60_001890"
     CDS             306..626
                     /locus_tag="VQM60_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081714.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(656..994)
                     /locus_tag="VQM60_001891"
     CDS             complement(656..994)
                     /locus_tag="VQM60_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081715.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(1006..1725)
                     /gene="trmD"
                     /locus_tag="VQM60_001892"
     CDS             complement(1006..1725)
                     /gene="trmD"
                     /locus_tag="VQM60_001892"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET1081716.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(1715..2233)
                     /gene="rimM"
                     /locus_tag="VQM60_001893"
     CDS             complement(1715..2233)
                     /gene="rimM"
                     /locus_tag="VQM60_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET1081717.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(2264..3052)
                     /locus_tag="VQM60_001894"
     CDS             complement(2264..3052)
                     /locus_tag="VQM60_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET1081718.1"
                     /translation="MKRFEVSTEIGSLSVAYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEALEELGISEYILCVHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(3156..3399)
                     /locus_tag="VQM60_001895"
                     /pseudo
     CDS             complement(3156..3399)
                     /locus_tag="VQM60_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
BASE COUNT          991 a          762 c          563 g         1112 t
ORIGIN      
        1 ctttaaaggc atccaccatc gtttgaaatt cttcatttga gagagtaatc cctttgccca
       61 ttttagtatg gtctggactc caagcacgaa tatcaaactt tgcaggggca ccattaaagc
      121 tcacacggtt aatttccttg gtccaacctt tttcgttttc agaaagagtc aacaagtgct
      181 cttcgatttc aaatgtaaat tctgccattt tcttctcctt ttttagtttc attagtttat
      241 tcgtaaaatc ttgtagattt taggaaaatt ttatataata ttgatataaa agaagggagg
      301 ccaatatgag acataaattc cagcaagttc taaataaaat acatgatttt ttaaatggat
      361 atgaccaacc tgaccagact gaaaccaact cccttacagc cactattgaa gaggctatcc
      421 agaaacaaac cgctgttcac cttatcttgt ctgagacaag ctttacaggt gacatcatca
      481 aatatgatca gcaaggccag caaattatcg tgaaaaattt ttccaaaaat gtgagccgga
      541 ttatccgtat aagcgatatt caacgcctgc gatttgtccc ctcaactgtc caaacagccc
      601 aaaaaaatag atttaagaaa gagtgagatg tagttgcttc atcccactct ttttcttagc
      661 gaatttgttc aaaatgtaaa tgaactgcaa tatgatctcc ataaccactt ctttccaagt
      721 cacgttgtaa acgataggaa atgtagtgtt ctgcaatggt aatgtaacct gcgcccaata
      781 aacgatgttc aaccatagat tgaatcatac tgatagtcgc acgttccacc ttggcttctt
      841 gtaaatccaa aactaccttc ttagtgactt gagcaagatt ttgacgcaaa tcatctgtca
      901 aaacataaac agtttgggct gccttcaaga tggcttggta aatcttatct ggattaaatt
      961 cagcaatttc gccattacgt ttgattactt gcataggttt ctcctttatt ctttgttttc
     1021 tttgatttct gccagcattt tttcttcttc tactgtcagt tgataatgtt caagtaaatc
     1081 tggtctgcgc tcgtaggttt tctttaaact ctcgtacaat cgccactgac gaatcttttc
     1141 atggtggcca ctcatcaata catctggcac gaccatgcct cgataatcat agggacgtgt
     1201 gtactgagga tattctaaaa gacctgaaga aaaactatca tcttggtggc tagactcctt
     1261 gccaatcact tctggaatca ggcgaactgt agcatcaatc atggtcatag ctgccaattc
     1321 tccaccagtg aggacatagt cgcctaggga aatctcatct gttaccaagg tcttaatgcg
     1381 ctcatcataa ccctcatagt gcccacagat aaagattagc tcttcctctt gagccaaatc
     1441 ttcagcataa gcctgatcaa actgctttcc agcaggatcg aggagaataa cgcgcggatt
     1501 tttcttttca atagcatcaa aggaatcgaa aataggttgt gctctgagca acatgccctg
     1561 accgcctccg tagggctcat catctacatg acgggccttt tcagcatttt ctcgaaaatt
     1621 atgatactgg atatccaaga gccctttttc tcgagccttt ccaacgattg agtgctccag
     1681 tggagaaaac atctctggaa agagggttaa aatatcaatc ttcatcgtct aacccttcta
     1741 agatttccac atcgacccgt ttatttggaa tatcaacatt gagaaccact ggtgggatat
     1801 aaggtaaaag caaatcacgt ttgccttttc gtttgaccac ccagacatca ttagcacctg
     1861 gttgcaggat ttccttgatg gttccaacca agctatcacc ctcatagact tccaaaccga
     1921 taatctcgtg atagtaaaat tcaccatcgt ctaggtcatt caaatcttcc tcagcgacct
     1981 tgagactgta tcccttgtac ttttcgatag tattgatatg gtacatatct ttgaatttaa
     2041 taatgtcaaa gttcttctgt ttacggtggc tagcgatggt cactgtttgg acaaactgat
     2101 ctttttcatc aaacaaagcc agctcagctc cttttttaaa ccgttcttct gcaaaatccg
     2161 tcacagacaa gactcgcatc tcaccctgta atccctgcgt attaacgatt ttcccaacat
     2221 taaagtagtt catcttgtct cctgtaatct ccttttttcc atcttattct gacaattctc
     2281 gaataatagc cgcaattttt tccgattctg accattgtaa ataatggtga ttccctccta
     2341 aaatgagttt agtattggaa gtccaatatt ctgattctct gtactctttt tctctataag
     2401 tctgacaaaa aataaataca gggacatgag attctatcga caaattctca aaatcttcct
     2461 cagtaatctc tccagatatc tgaaattctg gatcttgatt ttccaactct aagccttttt
     2521 cttgcattaa ttcccagatt tttttattcg tttcaggact aaatgttgct tgagttaagt
     2581 tcttaaaata aagttcagga ccacactcgt caatcagcct catctgctct tccatttctg
     2641 gataaggatt ttctgaaaaa tcagcaaaca tgactttttt agttgtcggt tcaattgcta
     2701 ctaaagtctg acacttaatt ggtttctcga gtaatttgca agctaaaatt ccactccaac
     2761 tatgtacaca aagtatatat tcagaaatcc ctaattcttc aagtgcttca taaaccacat
     2821 ctgcaagatt atctagattt tttccagctt ggtcatgaat cggactccta cctgtgttcg
     2881 gaaaatcaat tgtcaaataa ccaattgtag gaggaggttt ttcaagtata agtgaaaaat
     2941 tttcataact tggtagcaaa cctgctccgt ttagacaaac tagcactttc ttttgctttt
     3001 gataagcaac agagagacta ccaatttctg tagatacttc aaacctcttc ataaagaaat
     3061 ccactgattc tatataatga attattaaaa atccttatcc tttattttat cacgttccaa
     3121 ggattttctc aagttggagg aaggggacaa catctctact ttcccttcaa gaatccttcc
     3181 aaatcatgtt tatgttggta attaacggct gcggttttgt ctttctcaaa gatggttcta
     3241 gttaggtcaa tatgcttgat agcagcaata gcgacggtgt agtgaatgat atcagccagt
     3301 tctctggcaa gttcctcgtt cgaatcctat cccttctttt cgaccagagc gcctattcaa
     3361 aacctcggct acttctccga cttcctccac taacttcata aagagacctt catcagtccg
     3421 agactgct
//
LOCUS       DAVAAL010000154         3355 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVAAL010000154 DAVAAL010000000
VERSION     DAVAAL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.156"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            200..853
                     /gene="rr06"
                     /locus_tag="VQM60_001896"
     CDS             200..853
                     /gene="rr06"
                     /locus_tag="VQM60_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET1081719.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            850..2181
                     /gene="hk06"
                     /locus_tag="VQM60_001897"
     CDS             850..2181
                     /gene="hk06"
                     /locus_tag="VQM60_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET1081720.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL"
     gene            2276..2803
                     /locus_tag="VQM60_001898"
     CDS             2276..2803
                     /locus_tag="VQM60_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET1081721.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            2882..>3355
                     /locus_tag="VQM60_001899"
     CDS             2882..>3355
                     /locus_tag="VQM60_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HET1081722.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEEVGGRN
                     TPTVTSSGQDISKKYADEVESHLKKILSEIQTQLDRKRHTKTVALINELQNIKKTYLY
                     NLNVLKEKSELPSKIKAKLEVAFDQFKKDTLKPGEKVAEAEKKVAEAKKKAEDQKE"
BASE COUNT         1132 a          448 c          701 g         1074 t
ORIGIN      
        1 gagctgacgt agtttgaaga gattttcgaa gagtataagc tgcaagatga atgattttct
       61 tgtattgacg ttgttgttga caaaaagtag cggataaatg aaatccattc cattatcata
      121 gatgataggc tggtaggaaa ttttcaaata gcatacagga aatagatgta tggagttctg
      181 gtagtagaaa gggagagaga tgaacatttt agttgcagat gacgaggaaa tgattagaga
      241 aggaattgca gcatttctga cagaagaggg ttatcatgtc attatggcta aggatggaca
      301 agaggtcttg gaaaaatttc aagatctccc tatccatctc atggtactgg atttaatgat
      361 gcctaggaag agtggttttg aagtgttaaa agaaatcaat caaaagcacg atattcctgt
      421 catcgtcttg agtgctctgg gagatgaaac tactcagtca caggtatttg atctctatgc
      481 tgatgatcat gtgacaaaac ctttttcttt ggtactgctt gtcaagcgta ttaaggcgct
      541 tatcagacgt tactacgtca tagaggatct ttggcgatat caggatgtaa cagtggattt
      601 tacctcttac aaagcacatt ataaaaatga agaaattgat ctcaaaccaa aggaattact
      661 ggtactaaag tgtttgattc agcataaaaa tcaagtttta agtagagagc agatattgga
      721 agaaatttca aaagatgtag ctgatttacc ttgtgatagg gtcgttgatg tctatattcg
      781 tactcttcgc aaaaaattag ctttagattg tatcgtgact gtgaaaaatg ttgggtataa
      841 gattagctta tgataaaaaa tcctaaatta ttaaccaagt cttttttaag aagttttgca
      901 attctaggtg gtgttggtct agtcattcat atagctattt atttgacctt tcctttttat
      961 tatattcaac tggaggggga aaagtttaat gagagcgcaa gagtgtttac ggagtattta
     1021 aagactaaga catctgatga aattccaagc ttactccagt cttattcaaa gtccttgacc
     1081 atatctgctc accttaaaag agatattgta gataagcggc tccctcttgt gcatgacttg
     1141 gatattaaag atggaaagct atcaaattat atcgtgatgt tagatatgtc tgttagtaca
     1201 gcagatggta aacaggtaac cgtgcaattt gttcacgggg tagatgtcta caaagaagca
     1261 aagaatattt tgcttttgta tctcccatat acatttttgg ttacaattgc tttttccttt
     1321 gttttttctt atttttatac taaacgcttg ctcaatcctc ttttttacat ttcagaagtg
     1381 actagtaaaa tgcaagattt ggatgacaat attcgttttg atgaaagtag gaaagatgaa
     1441 gttggtgaag ttggaaaaca gattaatggt atgtatgagc acttgttgaa ggttattcat
     1501 gagttggaaa gtcgtaatga gcaaattgta aaattgcaaa atcaaaaggt ttcctttgtc
     1561 cgtggagcat cacatgagtt gaaaacccct ttagccagtc ttagaattat cctagagaat
     1621 atgcagcata atattggaga ttacaaagat catccaaaat atattgcaaa gagtataaat
     1681 aagattgacc agatgagcca cttattggaa gaagtactgg agtcttctaa attccaagag
     1741 tggacagagt gtcgtgagac cttgactgtt aagccagttt tagtagatat tttatcacgt
     1801 tatcaagaat tagctcattc aataggtgtt acaattgaaa atcaattgac agatgctacc
     1861 agggtcgtca tgagtcttag ggcattggat aaggttttga caaacctgat tagtaatgca
     1921 attaaatatt cagataaaaa tgggcgtgta atcatatccg agcaagatgg ctatctctct
     1981 atcaaaaata catgtgcgcc tctaagtgac caagaactag aacatttatt tgatatattc
     2041 tatcattctc aaatcgtgac agataaggat gaaagttccg gtttgggtct ttacattgtg
     2101 agtaatattt tagaaagcta tcaaatggac tatagttttc tcccttatga acacggtatg
     2161 gaatttaaga ttagcttgta gacagattag ttttttatta aagttcacat agggttaaca
     2221 taagtgtatt attctttata tagataaaaa tatagataaa agaaaggata ctaatatggt
     2281 attagcgatt attttagtaa cattccttat tcgattgatt tttttaaagc gttcgataga
     2341 gaatgagaaa cgaatcctta gcaatggcgg gaaagaattt ggagttgaga atacaaaacg
     2401 attaacgttg gctcatattg ttttttatct ctcttgcctc gttgaagcaa tggtgcacaa
     2461 gacaattttt gatggcatgg gcatggttgg tttagtcttg cttatttttt ctatgctgat
     2521 gttgatgttg gtgattcact tgttgggaga tatttggaca gtgaagctta tgcttgtcaa
     2581 taatcacaaa tatgtagatc atatcttgtt taggacagta aaacatccta attacttttt
     2641 aaatattctt cctgagttga ttggcttgac cttgttgagt catgcttatg tgacttttgt
     2701 tttagttttt ccagtttatg cagttatttt gtatcgacga atagctgaag aggaaaagct
     2761 attacatgaa gttataatcc caaatggaag cataaagaga taaatacaaa attcgattta
     2821 tatacagttc atattgaagt gatatagtaa ggttaaagaa aaaatataga aggaaataaa
     2881 catgtttgca tcaaaaagcg aaagaaaagt acattattca attcgtaaat ttagtattgg
     2941 agtagctagt gtagtagttg ctagcttgtt cttaggagga gtagttcatg cagaagaggt
     3001 tggtggtagg aataccccca cggttacatc tagtgggcaa gatatatcga agaagtatgc
     3061 tgatgaagtc gagtcgcatc taaaaaaaat attgagtgag atccaaacac aattagatag
     3121 aaaacgacat accaaaactg tagcgctaat taacgagttg caaaacatta agaaaacgta
     3181 tttgtataat ttaaatgttt taaaagagaa gtctgagttg ccgtcaaaaa taaaagcaaa
     3241 gttagaagta gcttttgatc agtttaaaaa agatacattg aaaccaggag aaaaggtagc
     3301 agaagctgag aagaaagttg cagaagctaa gaaaaaagcc gaggatcaaa aagaa
//
LOCUS       DAVAAL010000155         3354 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVAAL010000155 DAVAAL010000000
VERSION     DAVAAL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.157"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            44..1378
                     /locus_tag="VQM60_001900"
     CDS             44..1378
                     /locus_tag="VQM60_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET1081723.1"
                     /translation="MPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDT
                     EKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAIS
                     NYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLAEQQRII
                     EAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLL
                     EKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYGNKDETTSYPIYEIPEAWRYIKF
                     ASLVNFRIGKTPPRSEATFWGTEIPWVSISDMPISDMPISGYVTNTRESISKLALKSK
                     KIDISPKGTLLMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLI
                     STLGDSKDAIKGKTLNSTSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(1326..1931)
                     /locus_tag="VQM60_001901"
     CDS             complement(1326..1931)
                     /locus_tag="VQM60_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET1081724.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVATSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK"
     gene            complement(1942..2739)
                     /gene="psrA"
                     /locus_tag="VQM60_001902"
     CDS             complement(1942..2739)
                     /gene="psrA"
                     /locus_tag="VQM60_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HET1081725.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            complement(2796..>3354)
                     /locus_tag="VQM60_001903"
     CDS             complement(2796..>3354)
                     /locus_tag="VQM60_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET1081726.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYAWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
BASE COUNT         1171 a          554 c          513 g         1116 t
ORIGIN      
        1 gtttgctgat ggaagcactc aagaaattga tgttccttat gatattcctg atacttggga
       61 gtgggtgagg ttttctacat tggttgaaat tgtcagaggt ggctctccac gaccaatcaa
      121 agattatctt acttctgaag tagatggaat aaattggata aaaataggtg atactgaaaa
      181 gggtgaaaag tatataaata atgttaaaga aaaaatcaaa aaatcagggc ttaacaaaac
      241 tagatttgta aaaaaaggta catttttgtt aactaattct atgagttttg gtagacctta
      301 tattttgaat gttgatggtg caatacacga tggatggttg gctatttcga actatgaaaa
      361 ctcattaaat aaagattacc tattctatat tctttcatca aatgtagttt attctcaatt
      421 tctatctcta attagtggag ctgttgtgaa aaacttgaat agtgataaag ttgcttctat
      481 tcttatccct ctccctccgc tagctgaaca acaacgcata atagaagcaa tcgaatcagc
      541 tttagaaaaa gtagatgaat atgctgaaag ttataataga ctagaacagc tagataaaga
      601 atttccagat aaactaaaaa aatctattct tcaatatgct atgcaaggaa aattagttga
      661 acaagaccca aatgatgaat cagtcgaagt tttacttgaa aaaatacgag cagaaaaaca
      721 aaaactcttt gaagaaggca agattaaaaa gaaagatttg gacatttcta ttgtttccca
      781 aggagatgat aactcttatt atgggaacaa agacgaaaca acctcttatc ctatatatga
      841 aatcccagaa gcatggagat atattaaatt tgctagccta gttaacttca gaataggaaa
      901 aactcctcca cgcagtgaag ctactttttg gggaactgaa ataccctggg tatctatatc
      961 agatatgcct atatcagata tgcctatatc tggttatgta actaatacga gagaatctat
     1021 ttctaaatta gcattaaaat caaagaaaat agacatttca cctaaaggaa ctttattaat
     1081 gagttttaaa ttatctattg gaaaagttgc catattggat attcctgcca ctcataatga
     1141 agccattata tcgatattcc cctatgctaa caaagaaaat attatcagag attacttaat
     1201 gatatttttg ccacttatct ctactttagg tgattcaaaa gatgctatca aagggaaaac
     1261 attaaatagt accagtatct ccgaattatt aatccctatt tctaatcatg aagagatgaa
     1321 aagaatcatt tccaaagttg atttactttt tcaaaaagtt tctcaacttt ttgagtaata
     1381 agttcctgtt cctcaaaagg agctaacgga attaatagct cgcttagtgt agttttagga
     1441 atattatata aagcttgacc tgatagttta gttattgctt tcaattgttt ataaaataac
     1501 ggagaggaca agttaaataa tagaaatttt gaaataatct ctgaactttc gaatggtgtt
     1561 aattggaaaa taaatccacc agccacaaca ccatcatagt ctttatcgat tcttgcaaac
     1621 tttccaatat gttctaaaga ggttgctaca ggtgttatta gctgattatg ttttaaataa
     1681 acttgctcag aggagatgaa ttgtgtatca atgtagtaat cattatccaa cagagaaaat
     1741 tctaaaggct taatattacc accacgtata attctaacac ctttattatt aatgcttaaa
     1801 tcgcccttct tgtaagaaag acctgtattc attgaaaaaa tatcttttat ttttataaca
     1861 acccaattca taggtatatt cccataataa gagccataat cacaaaaata gcaggtagtc
     1921 agtttgacca cctgttattt tttaccaatt aacaatttta tctacaatat tttgttgttc
     1981 agtagctgtt ttccttagat aaattcgagt agtttctata ctttcgtgtc ccatcaaatc
     2041 tgcaagcaag gcaatatcat tatacttcgc taaaaaattc ttagcaaata aatgcctaaa
     2101 agaatgaggg taaattactt taggattcat tttgtattta tcagcataat tttttaactg
     2161 ttgagcaact cctcttgctg taattggttc gttaaattta ttcaaaaata aataaccact
     2221 tcggcgattt tctgattcta accaactaag acaactattt cttaattttt taggaatgta
     2281 cagtctacga attttaccac cttttgagta aatgtcaaaa taaccgattt ctacatgctc
     2341 tacttttagt ttaataagtt cacttacacg agccccagtt gcacctaaaa accaaacgac
     2401 aaaatgccat tttaaaatac catctttttt caaactacgt ttaagaaaaa ggtaatcagc
     2461 atggctaatg acatcttcta aaaacggttt ttgctgtact ttgacaaatt ttaatttcaa
     2521 atcatcatga ccaataaaag ccagatattt atttactcct tgtagtcgca aattgacagt
     2581 tttaggttta aaattgtcta ataaatatcc tttgtattca aataaatctt ccattttgag
     2641 ttcgtaattc tccaagaaaa atcgaacacc ataaaggtac gaacgcacag tattttcagc
     2701 taaaccagct ttcttcaaat gtaattcaaa atctttcaac gtaaaactcc tatcttatgt
     2761 ttgatagaaa ttccaccgca cgtaaaacta ttatactaaa ttagtgcgtc aatatgggcg
     2821 aaaaattgtt cgattttatc aacgattctg gattgttcag gaaggggtgg gagggggatt
     2881 aaatattctt ttatagtttt cgttaataat tctttttgtt ttgtactacc cgacgctttt
     2941 tcttcaataa ctgactgaac aataggagag gaaagaaaat tatagatgaa atggcaatta
     3001 ataacccccg ataagactct tataactgta acatggctat ctgcaacagc ccaggcataa
     3061 ggatttttat tttcatggta aatagctaat cgtcctaacg tacctagacc tgttgaattc
     3121 cacattaaat caccatccct tagtaatctt tctttctggt aactatgaac tgtttcggga
     3181 tcaataaatc ttgctaagtc aatagaaaag ccagaccatt gattacattt ctgagcaatc
     3241 acagggtata taggaatatt tgaatatttt ggagacttcc ctctttgaat gtaggaggtt
     3301 atatcgttta acctcaccca ttcccaactt tctggtattt cacaaggtac ttcc
//
LOCUS       DAVAAL010000156         3257 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVAAL010000156 DAVAAL010000000
VERSION     DAVAAL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.158"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            85..2280
                     /locus_tag="VQM60_001904"
     CDS             85..2280
                     /locus_tag="VQM60_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET1081727.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENISLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            2333..2485
                     /gene="rpmG"
                     /locus_tag="VQM60_001905"
     CDS             2333..2485
                     /gene="rpmG"
                     /locus_tag="VQM60_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET1081728.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            2495..2671
                     /gene="secE"
                     /locus_tag="VQM60_001906"
     CDS             2495..2671
                     /gene="secE"
                     /locus_tag="VQM60_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET1081729.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            2726..>3257
                     /gene="nusG"
                     /locus_tag="VQM60_001907"
     CDS             2726..>3257
                     /gene="nusG"
                     /locus_tag="VQM60_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET1081730.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         1053 a          558 c          720 g          926 t
ORIGIN      
        1 agatattgta acaaaaaaag ccctatttgg ataggaaata gctaaattct tgtcttccta
       61 tgatgaagat gataaaataa acgcatgaaa ttagataaat tatttgagaa atttctttct
      121 ctttttaaaa aagaaacaag tgaactagag gactctgatt ctactatctt acgtcgctct
      181 cgtagtgatc gaaaaaaatt agcccaagta ggtccgattc gaaaattctg gcgtcgttat
      241 catctaacaa agattatcct tatactaggt ttgagtgcag gattgctagt tggaatctat
      301 ttgtttgctg tagccaagtc gaccaatgtc aatgatttgc aaaatgcctt gaaaactcgg
      361 actcttattt ttgaccgtga agaaaaagag gctggtgcct tgtctggtca aaagggaacc
      421 tatgttgagc tgactgacat cagtaaaaac ttgcagaatg ctgttattgc gacagaagac
      481 cgttctttct ataaaaatga cgggattaac tatggccgtt tcttcttggc tattgtcact
      541 gctggacgtt caggtggtgg ctctaccatt acccaacagc tggctaaaaa cgcctattta
      601 tcgcaggatc aaactgttga gagaaaagcg aaagaatttt tccttgcctt agaattaagc
      661 aaaaaatata gtaaggagca aattctaacc atgtacctta acaacgctta ttttggaaat
      721 ggtgtgtggg gtgtagaaga tgcgagtaag aaatactttg gagtttctgc atcagaagtg
      781 agtctggatc aagctgcgac tctggcaggg atgctcaagg ggccggaact gtataatccc
      841 ttgaattctg tagaagattc tactaatcgg cgcgatactg tcttgcagaa tatggttgca
      901 gcaggatata ttgataaaaa ccaagaaacc gaagctgctg aagttgatat gacttcgcaa
      961 ttgcacgata agtatgaagg aaaaatctca gattaccgtt acccctctta ttttgatgcg
     1021 gtggttaatg aagctgtttc caagtataat ctaacagagg aagagattgt caataatggc
     1081 taccgcattt acacagagct ggaccaaaac taccaagcaa atatgcagat tgtttatgaa
     1141 aacatatcgc tatttccgag ggcagaggat ggaacgtttg ctcaatcagg aagtgtagct
     1201 ctcgaaccga aaacaggggg agttcgtgga gttgtcggtc aagttgctga caatgataaa
     1261 actggattcc ggaatttcaa ctatgcaacc caatcaaagc gtagtcctgg ttctacaatt
     1321 aagcctttag ttgtttatac gccagcagtt gaagcaggct gggctttgaa taagcagttg
     1381 gataaccata ccatgcagta tgacagctat aaggttgata actatgcagg gatcaaaacg
     1441 agtcgagaag ttcctatgta tcaagccttg gcagaatcgc ttaatctacc tgctgttgcc
     1501 actgttaatg atttgggtgt cgacaaggct tttgaggcag gcgaaaaatt cggactcaac
     1561 atggaaaagg ttgaccgtgt tcttggtgtc gccttgggaa gcggtgttga aaccaaccct
     1621 cttcaaatgg ctcaagcata cgccgccttt gcaaatgaag gtttaatgcc tgaagctcat
     1681 tttattagta gaattgaaaa tgctagtgga caagttattg cgagtcataa aaattcacaa
     1741 aaacgggtga ttgataagtc tgtagctgac aagatgacca gtatgatgtt ggggactttc
     1801 accaacggta ccggtattag ttcatcgcct gcagactata ttatggcagg gaaaactgga
     1861 acaactgaag cagtttttaa tccggagtac acaagtgacc agtgggtaat tggttatact
     1921 ccggatgtag tgattagcca ctggcttggc tttccgacca ctgatgaaaa ccactatcta
     1981 gctggctcta cttcaaacgg tgcagctcat gtctttagaa acattgccaa taccatttta
     2041 ccttatacgt caggaagtac ctttacggtt gaaaatgctt ataagcaaaa tggaattgca
     2101 ccagccaata caaaaagaca agtacaaacc aatgataata gccagacaga tgataatttg
     2161 tctgatattc gagggcgtgc gcaaagtcta gtagatgagg ctagccgggc tatctcagat
     2221 gcgaagatta aggaaaaggc tcaaacaata tgggattcga tagtcaatct atttcgctaa
     2281 gatgcttgtc aaagcctagc tttcttgtta taatggataa gatggaggcg ttatggcact
     2341 aaaaaaagca agcctagctt gtgcggtttg tggttcgaga aactattcaa tcaagatcag
     2401 cggaaacccc aagcctacac gactagaagt aaataaattt tgtaagcatt gtggcaagta
     2461 cactacacat agagaaacga gataggagag agcgatgcgt tttattggag atatttttag
     2521 acttcttaaa gacacaacgt ggccaactcg caaggaaagc tggagagatt ttcgttctat
     2581 catggaatac acagctttct ttgtagtaat tatttacatt tttgaccagt tgattgtttc
     2641 aggtttgatt cgatttatta acatttttta gaagattggt ggagttaatt acactagaaa
     2701 tcttctattt atgaaaggaa atatcatgga tagttttgat aaaggatggt ttgttttaca
     2761 aacttattct ggttatgaaa ataaggtgaa agaaaatcta ttacaacgtg cacaaaccta
     2821 caatatgttg gataatattc tacgcgttga aattccaaca caaacagtgc aagttgaaaa
     2881 aaatggaaag agaaaagaag tagaagaaaa tcgctttcca ggttatgttc ttgtagaaat
     2941 ggtcatgaca gatgaagctt ggtttgttgt tcgaaacaca ccaaatgtta caggatttgt
     3001 cggatcacac gggaacagat caaaaccaac tccattattg gaacaagaaa ttcgtgacat
     3061 tttggtatct atgggacaaa ctgttcaaga atttgatttc gatgttgaga ttggtcaaac
     3121 tgtacgtatt attgatggtg cttttgcaga ctacactggt aagattacag aaattgataa
     3181 taacaaagtg aaaatgatta tctctatgtt tggtaatgac acagttgcag aagtaaacct
     3241 aaaccaaatc gcagaat
//
LOCUS       DAVAAL010000157         3235 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVAAL010000157 DAVAAL010000000
VERSION     DAVAAL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.159"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            298..2949
                     /gene="adhE"
                     /locus_tag="VQM60_001908"
                     /gene_synonym="adhC"
     CDS             298..2949
                     /gene="adhE"
                     /locus_tag="VQM60_001908"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET1081731.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         1048 a          655 c          648 g          884 t
ORIGIN      
        1 cctccttatc aaaggaggta ttattagaat cacaaggtaa gcgcttacct gtaaatccct
       61 ttctttccaa atccctcttc caagcaagtt tgtgaaataa aaaatatttg aaataaattt
      121 cacaaacttc aaagataaaa cttgataaga aaagaaaatg agaaaagttt cacaagagtt
      181 taaaaattct ttgtgaaatg tttatgaaac tgtttacaaa gttgaaataa tgcgttataa
      241 taaacttgtg aaaaaattaa caaaggatat cgttccttga aagctatgga ggaaaatatg
      301 gctgataaaa aaactgtgac accagaggaa aagaaactcg ttgctgaaaa acacgtagat
      361 gagttggttc aaaaagctct agttgccctt gaagaaatgc gtaaattgga tcaagaacaa
      421 gttgactaca tcgttgccaa agcatcagta gcagctttgg atgcccacgg agaattggct
      481 ttacatgcct ttgaagaaac aggacgtggt gtatttgaag acaaagcaac taagaacttg
      541 tttgcctgtg aacacgtagt aaacaacatg cgccacacta agacagttgg cgttatcgaa
      601 gaagacgatg taacaggatt gactcttatt gctgaaccag ttggtgttgt ttgtggtatt
      661 actccaacaa caaacccaac atcaacagca atcttcaaat cattgatttc attgaagaca
      721 cgtaacccaa tcgtctttgc cttccatcca tcagcacaag aatcatctgc tcatgcagct
      781 cgtatcgtcc gcgatgcagc tatcgcagct ggtgctcctg aaaactgtgt gcaatggatt
      841 actcaaccat ctatggaagc aacaagtgcc cttatgaacc acgaaggtgt tgcgacaatc
      901 cttgcaacag gtggtaatgc catggttaag gcggcttatt catgtggtaa accagctctt
      961 ggggtaggtg ccggaaacgt tccagcttat gttgaaaaat cagcaaacat tcgtcaagca
     1021 gcacacgata tcgtcatgtc taaatcattt gataacggta tggtctgtgc atctgaacaa
     1081 gcagttatca ttgataaaga aatttacgat gaatttgtag cagagttcaa atcttaccac
     1141 acttactttg taaacaaaaa agaaaaagct cttcttgaag agttctgctt cggcgtcaaa
     1201 gcaaacagca aaaactgtgc tggtgcaaaa ttgaacgctg acatcgttgg taaaccagca
     1261 acttggattg cagaacaagc aggatttaca gttccagaag gaacaaacat tcttgctgca
     1321 gaatgtaaag aagttggcga aaatgagcca ttgactcgtg aaaaattgtc accagttatt
     1381 gcagttttga aatctgaaag ccgtgaagat ggtattacta aggctcgtca aatggttgaa
     1441 tttaacggtc ttggacactc agcagctatc cacacagctg acgaagaatt gactaaagaa
     1501 tttggtaaag ctgttaaagc tattcgtgtt atctgtaact caccttctac ttttggtggt
     1561 atcggggacg tttacaatgc cttcttgcca tcattgacac ttggatgtgg ttcttacgga
     1621 cgcaactcag ttggggataa cgttagtgcc attaacctct tgaatatcaa aaaagtcgga
     1681 agacggagaa ataacatgca atggatgaaa cttccttcaa aaacatactt tgaacgtgat
     1741 tcaattcaat accttcaaaa atgtcgtgac gttgaacgtg tcatgatcgt tactgaccat
     1801 gccatggtag agcttggttt ccttgatcgt atcatcgaac aactggacct tcgtcgcaat
     1861 aaggttgttt accaaatctt tgcggatgta gaaccggatc cagatatcac aactgtaaac
     1921 cgtggtactg agattatgcg tgccttcaaa ccagatacca tcatcgcact cggtggtggg
     1981 tctccaatgg atgctgccaa agtaatgtgg ctcttctacg agcaaccaga agtggacttc
     2041 cgtgaccttg tccaaaaatt catggatatc cgtaaacgtg ccttcaagtt cccattgctt
     2101 ggtaagaaga ctaaattcat cgcgattcca actacatctg gtacaggatc tgaagtaaca
     2161 ccatttgccg ttatctctga taaagcaaac aaccgtaaat acccaatcgc tgactactca
     2221 ttgacaccaa ctgtggcaat cgtagatcct gctttggtat tgacagttcc aggatttgtt
     2281 gctgctgata ctggtatgga cgtattgact cacgcgacag aagcatacgt atcacaaatg
     2341 gctagtgact acactgatgg tttagcactt caagccatta aattggtctt tgaaaatctc
     2401 gaaagctcag ttaagaatgc agacttccac tcacgtgaga aaatgcataa cgcttcaaca
     2461 atcgctggta tggcctttgc caatgccttc ctaggtattt ctcactcaat ggcccataag
     2521 attggtgcgc aattccacac aatccacggt cgtacaaatg ccatcttgct tccatacgtt
     2581 atccgttaca acggtacacg tccagctaag acagcaacat ggcctaagta caactactac
     2641 cgtgcagatg aaaaatacca agatatcgca cgcatgcttg gacttccagc ttctactcca
     2701 gaagaagggg ttgaatctta cgcaaaagct gtctacgaac tcggtgaacg tattgggatc
     2761 caaatgaatt ttagagacca aggaattgac gaaaaagaat ggaaagaaca ttctcgtgaa
     2821 ttagccttcc tggcttatga agaccaatgt tcaccagcta acccacgtct tccaatggta
     2881 gaccatatgc aagaaatcat cgaagatgca tactatggct acaaagaaag accaggacgc
     2941 cgtaaataat tgtttatcag tctagaagca agacaaaaac tcaatttgag ggaaagaacc
     3001 agtaattttt ctatgataaa aggcatccta tcaaggtttt tgaatacctg ataggatgcc
     3061 tttttatgat gttgaggcct ttttgccctt tttgaaaaac tagaatagaa acaaaatata
     3121 tagtaggttg aaactagaat agtacacatc tgcttctaaa acattgttag aattcgattt
     3181 gactgtcctg atcgatttgt cctgttctta tttcatttta atatataaaa aatat
//
LOCUS       DAVAAL010000158         3138 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVAAL010000158 DAVAAL010000000
VERSION     DAVAAL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.160"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            87..1049
                     /locus_tag="VQM60_001909"
     CDS             87..1049
                     /locus_tag="VQM60_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET1081732.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETISKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIA
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1229..2236
                     /gene="gap"
                     /locus_tag="VQM60_001910"
     CDS             1229..2236
                     /gene="gap"
                     /locus_tag="VQM60_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET1081733.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2305..3060)
                     /locus_tag="VQM60_001911"
     CDS             complement(2305..3060)
                     /locus_tag="VQM60_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET1081734.1"
                     /translation="MINQEEVHWNEIVNPGDVCQLTFDEEDYSQKTIPWGNPDLVQEV
                     YQDQHLIIVNKPEGMKTHGNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKN
                     PFILPILNRLLEKKEISREYWALVDGNINRKELVFRDKIGRDRHDRRKRIVDAKNGQY
                     AETYVSRLKQFSNKTSLAHCKLKTGRTHQIRVHLSHHNFPILGDPLYNSNSKTSRLML
                     HAFRLSFTHPLTLEKLNFTALSNTFETELKKNG"
BASE COUNT          936 a          567 c          662 g          973 t
ORIGIN      
        1 ttttatcctg ctcttgtttc aattgactat agttcacgga tacagttggg aaagaagcta
       61 ttaaatattt ggagagtcct tttgggatga acatgtcaaa tattactata gatgggcaag
      121 aaatggatct gagtcaaacg atgccttctt atgctcaagt agtgagtctt tatgatgata
      181 caagtatctc tgttagtaat aatgagacgg acaaagtgtt ggctggctcc ctctatactg
      241 atacaaatga acagggatta acgattccaa gcagtttact aaaaaattgg aatgaacaga
      301 caggaaaaaa tttgacagct aatgatctta ttggcaaatc agtctcagcc agcattgtag
      361 aaagtgctgc cgaaactagt aagattgctc aatttcaaac gaagattgta cgtgtaatca
      421 atgatgaaga tgacatggag gacagcaaca gtttcatgct gtctcatcaa atggaaacga
      481 tttcgaaaga ggctggattt acgaaagctg tatcttattt tatcttggaa ctcaaagatc
      541 catcacagac aaaagtagta acagaagaat tacagaaaaa taagaagtat actgtgcttt
      601 ctcaacagag agttcttgat attgtgatta cctttattcg tgttattcag ggattattga
      661 ttgtgctttc atcacaagct attgtggtag cagcggttat gattggtatc attatttaca
      721 tcaatatcat gcaacgttcc aaggaaatag gtgtcatgaa ggcagttggc tatcagaatc
      781 gtgatgtcaa aggaattttt atttacgagg ctatctggat tgtaggaatc gccttgctac
      841 tggctttttt ggtagcacaa ggggtgggaa gtttagcgaa tgcgattgta agtcactttt
      901 acccatccat cgctaaggtc tttgaattaa atttgttatc tgttttagga actctagttt
      961 tcgctctctt gcttggctat gtatcagcct acttcccagc acgtaaaatt agtaaaatgg
     1021 accctgtaga atcgctacgc tatgaatagt caagggtgtt actagattat cgtccctaaa
     1081 aaagaaacta acagagaaaa acaatgcccg tacttgcaat tcaactcaaa tagttatata
     1141 ataggtttgt tgaaaggtga tttgtagtga ttcaagttac tcttttcaca ataaaaattt
     1201 catgattttc ataaggagga aatcactaat ggtagttaaa gttggtatta acggtttcgg
     1261 acgtatcggt cgtcttgctt tccgtcgtat ccaaaacgta gaaggtgttg aagttacacg
     1321 catcaacgac cttacagatc cagttatgct tgcacacttg ttgaaatacg acacaactca
     1381 aggtcgtttc gacggtactg ttgaagttaa agaaggtgga ttcgaagtta acggtaaatt
     1441 cgttaaagtt tctgctgaac gtgatccaga gcaaatcgac tgggctactg acggtgtaga
     1501 aatcgttctt gaagctactg gtttctttgc taagaaagaa gcagctgaaa aacaccttaa
     1561 aggtggagct aaaaaagttg ttatcactgc tcctggtgga aacgacgtta aaacagttgt
     1621 attcaacact aaccacgacg ttcttgacgg tactgaaaca gttatctcag gtgcttcatg
     1681 tactacaaac tgcttggctc caatggctaa agctcttcaa gataactttg gtgttgttga
     1741 aggattgatg actactatcc acgcttacac tggtgaccaa atgatccttg acggaccaca
     1801 ccgtggtggt gaccttcgcc gtgctcgcgc tggtgctgca aacatcgttc ctaactcaac
     1861 tggtgctgct aaagctatcg gtcttgtaat cccagaattg aacggtaaac ttgacggatc
     1921 tgcacaacgc gttccaactc caactggatc agttactgaa ttggtagcag ttcttgaaaa
     1981 gaacgttact gttgatgaag tgaacgcagc tatgaaagca gcttcaaacg aatcatacgg
     2041 ttacacagaa gatccaatcg tatcttcaga tatcgtaggt atgtcttacg gttcattgtt
     2101 tgacgcaact caaactaaag ttcttgacgt tgacggtaaa caattggtta aagttgtatc
     2161 atggtacgac aacgaaatgt catacactgc acaacttgtt cgtactcttg aatacttcgc
     2221 aaaaattgct aaataattct tgaatcgata aaaagcaagg cctcttggtc ttgcttttct
     2281 tatatggaaa aatggatgac acaatcatcc attctttttt aattctgttt caaaagtatt
     2341 tgagagggca gtgaagttta atttttctaa tgtaagtgga tgggtaaagg aaagtcgaaa
     2401 ggcatgaagc ataagccgac ttgtctttga attgctgtta tagagagggt cgcccaaaat
     2461 agggaagtta tgatgcgaaa ggtgcacacg aatctgatgg gttcgccctg tctttagctt
     2521 gcaatgagcc aaggaagtct tgtttgagaa ttgctttaat ctgcttacat acgtttcagc
     2581 atattgccca ttttttgcat caactattct ttttctacga tcatggcgat cacgtccaat
     2641 tttgtctctg aaaacaagtt cttttctgtt gatatttcca tcaactagag cccaatattc
     2701 tctagaaatc tcttttttct ccaataagcg attgagaatg ggcaggataa aaggattttt
     2761 ggcaaagaga actaagccac tggtttccat gtccagacga tgaacgacat agcaggtttg
     2821 gccaacatag gtactgacat ggttaagaag ggcaatttcg tttggttgat taccatgcgt
     2881 tttcatcccc tctggtttgt ttacaataat caagtgttga tcttgataaa cttcctgcac
     2941 taagtctggg ttgccccaag ggatcgtctt ttgggaataa tcttcctcgt caaaagtcaa
     3001 ctggcaaaca tctccaggat ttacgatttc gttccagtgg acttcttctt gatttatcaa
     3061 aatatgtttc ttgattctca aaaaatgacg gatttttcta gggatgagga gttgttcctc
     3121 aagtaattgc tttaccgt
//
LOCUS       DAVAAL010000159         3062 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVAAL010000159 DAVAAL010000000
VERSION     DAVAAL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.161"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(43..1383)
                     /locus_tag="VQM60_001912"
     CDS             complement(43..1383)
                     /locus_tag="VQM60_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET1081735.1"
                     /translation="MYIFWIILYTLITNGLEIVIFFKVDGIGLTFERIFKAFLLKILL
                     AFVFVMIGYMIGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQEQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRREILDTGFQK
                     SLTKINWIMGAYYLVIQSLSYFEYEQGIQSTTVRHLILVFYLLFFMGVIKKLDTYLKD
                     KLHDRLDQEQVLRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYDLVLKDSSKKLQDNKYDLGRLVNVRDRALKSLLAGKFIKAREKDIVFNVEVPEEI
                     QVEGMRLLDFLTIVSILCDNAIEASAEAGQPHVSIAFLKSGAQETFIIENSIKEEGID
                     ISEIFSFGVSSKGEERGVGLYTVMKIVESYPNASLNTTCQDQVFRQVLTMIPTE"
     gene            complement(1396..2133)
                     /locus_tag="VQM60_001913"
     CDS             complement(1396..2133)
                     /locus_tag="VQM60_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1081736.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIREQDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRPHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(2138..2476)
                     /locus_tag="VQM60_001914"
     CDS             complement(2138..2476)
                     /locus_tag="VQM60_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET1081737.1"
                     /translation="MKHMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEKASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVRYARRRYRE
                     IRQKWLKEGE"
     gene            2752..>3062
                     /locus_tag="VQM60_001915"
     CDS             2752..>3062
                     /locus_tag="VQM60_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET1081738.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKTETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     N"
BASE COUNT          997 a          667 c          517 g          881 t
ORIGIN      
        1 aataagaccg agtaacaaga actctcggtc ttattttttt catcattctg taggtatcat
       61 agtaagcacc tgacgaaaga cttgatcttg acaggtagta tttaaactgg cattgggata
      121 actttccaca atcttcataa cggtatagag accgacacct ctctcctccc ctttagaact
      181 aactccaaag gagaagattt cagaaatatc tatgccctct tctttgatgg aattttcgat
      241 gatgaaggtc tcctgtgctc cactttttaa aaaggcaatt gaaacatgag gttgaccggc
      301 ctctgcactc gcttcaatag cattatcaca aaggatagac acaatggtta gaaaatcaag
      361 cagtctcatc ccctcaacct gaatctcttc aggaacttcg acattaaaga caatgtcctt
      421 ttctctagct tttataaatt ttccagctag gagacttttg agggcacgat cccgaacatt
      481 caccaatcgg ccaaggtcat atttattgtc ctgcaatttt ttactagaat cctttaagac
      541 caagtcatag acctctttta tctgctccat atcctcctct tcaataccca gacgtaaact
      601 tgtcaagagg ttggtgtagt catggcgaaa actccttact tccttgtaaa gttcctctat
      661 atgccgacta tagcgttcca tatctctata gcgcaagacc tgctcttggt ccagtctatc
      721 atggagtttg tccttcaaat aggtatccaa tttcttgata acccccataa aaaataggag
      781 gtaaaacact aggatgagat ggcgaacagt cgttgattga ataccttgct catattcaaa
      841 gtaagacaga ctttgtatca ccaaatagta agcccccatt atccagttaa tcttagtcag
      901 ggacttttga aaacctgtat ctagaatctc ccttctcaaa ctagtaaaat catagtccaa
      961 ccatttcaaa aaggctagag aaatgaagaa attgaaaatt attatacata acccagtaaa
     1021 tgagtagcca tcatatactt gctcttgtcc caaaaatgga agcacaaaat aggagactcc
     1081 tctataaaag agattcacca atatcattgg aaagagacca taaaagaaaa ggagtttttt
     1141 aggaagccct cttaacaata agaaagacaa gcctatgcca tacaagggtt ccataaaata
     1201 agataggtaa acatttccta tcatatagcc aatcattaca aaaacaaagg ccaacagtat
     1261 cttcaaaaga aaggccttaa aaatcctctc aaaagtgaga ccaattccat ctaccttaaa
     1321 gaaaatgaca atttctagcc cattagtaat aagtgtatac aatataatcc aaaaaatata
     1381 cataaatttc ctagttcagt gtaagttatt aatagcctca gacacttccc tgaccttata
     1441 acgcgcgatc agacagcttc caccattggg aaagaaaagg agtttttctt tcttatccaa
     1501 atgcaccaca tttgcaggat tgatgagaaa agagcgatgg cactgcaaga gacggggttc
     1561 ctgcttaaaa acctcctcta aactcgccgt aaattctagc ctgtccgtct tggtatagag
     1621 aataacacga tggggtcttg gggatgtttc gagatagtaa acctctttga aaggatattg
     1681 gaattgggca aattttgatt taaagtaaaa gcaatcttcc gccagacttt tactatcttg
     1741 actattggca tagaggaggg ctgtctcgat acgagattca aactcctctg ccgaaagggc
     1801 cttatcaatg tagtccaaag ctgacacttg gtagcgaaag gacagaggca taaactccga
     1861 gtgagtcgtc acaaagacga ttagggcata agggtcttgt tcccgaatct ttctagccac
     1921 ttccagtccc ttcatctctt catttcgaat ctcaatatcc aaaaagaata gctgatgggc
     1981 ccccttctca tgcacctctg ccagcagttg gtccggcttg ccaaatacct caaaagagct
     2041 aggaatgata tgatgtgctt tcaaaagttt ctcaatcgtc gtttcaattc tagtctgttg
     2101 ggaaaaatca tcttctaaaa caaatattct catcttctta ctctccctct ttcaaccatt
     2161 tttgacgtat ttctctatag cgacgtctgg catacctgac agcatattga ccttcttctc
     2221 cgagaaaaag gattctttct tgaaaatcaa tcgattttaa tttatcaaga ttaaccaaac
     2281 aatttcgatg acatctcatc aaagtttccc cacattggtt ctcaagatta cttaaatttt
     2341 gaagcatatt aaaactagct ttttctgtaa caatctgtac ggtatgggct ttagttggat
     2401 gtgtttggat atagtagata tcgtcgatgt ttactttata gactgttttc tgtgtctgaa
     2461 taatcatatg tttcattgga ttctcctcct aagaaaagaa taacagaaaa gatctagccc
     2521 aagcacaccc aatcctaaac agtctttgaa aactcctaaa aggattattt tctacaacat
     2581 catgatgagc ttacaactat tttatcataa aattcactca gcgcaattca agacatttca
     2641 atgacaatta aagatttgaa taaaaaaaaa gaattaaaag tgataaacta acagtatcaa
     2701 aacacagttg ccagaagtaa gactggcacc cagattaaag gaggcattcc catgacagat
     2761 acagacccca tcaaaagagc tcatactttg attactgact taaacaaagc ctaccaagca
     2821 tgcaaacagg caagcgctga cgacgtccgc tttcaggagc aattaaactc tattcttggt
     2881 tttctagcca agactgaaac agtggataat cgattcttga ttgaactaga aaaattttac
     2941 cagacttcca gtcttctcat gggactcagc gctcttgatc cagatgctcc aactcgcgcc
     3001 gcttggcggg cctatgaccg cttccacttt gaccaagtca agaccaagtt aatactcaat
     3061 ga
//
LOCUS       DAVAAL010000160         3059 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVAAL010000160 DAVAAL010000000
VERSION     DAVAAL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.162"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(50..583)
                     /locus_tag="VQM60_001916"
     CDS             complement(50..583)
                     /locus_tag="VQM60_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET1081739.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(672..1448)
                     /gene="recX"
                     /locus_tag="VQM60_001917"
     CDS             complement(672..1448)
                     /gene="recX"
                     /locus_tag="VQM60_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET1081740.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1486..2841
                     /gene="rlmD"
                     /locus_tag="VQM60_001918"
     CDS             1486..2841
                     /gene="rlmD"
                     /locus_tag="VQM60_001918"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET1081741.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIIDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            2903..2974
                     /locus_tag="VQM60_001919"
     tRNA            2903..2974
                     /locus_tag="VQM60_001919"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2936..2938,aa:Glu,seq:ttc)
BASE COUNT          982 a          554 c          573 g          950 t
ORIGIN      
        1 ttatactacc aaaaaaaggt caggattttg ttcctgacct ttgacaactt taccgattct
       61 ttagttctac atagcgcttg taccaaatgt ttacataggc ttctgagaaa ggaccacgtc
      121 cattgttaat ccaatcaaca agaattttga catgttcttt taaaatatag tccaagtcat
      181 cagaataatt cattttgcgt ttgtgacgct cgtactcttc aacgtccaag agacgttttt
      241 ccccatctgt aaaaatttta acatccaaat cgtaatcaat atacttcagt gcttcttcat
      301 ccagatagta ggggctagcc atattgcaat agtaagaagt tccattatca cgaatcatgg
      361 caatgatatt aaaccaatat ttcttgtgaa agtaaacaat agccggttct cgagtgaccc
      421 aacgacgacc atcactttcg gtaacaagtg tatgatcgtt gacaccaata atggcgtttt
      481 ctgttgtttt tagtaccatg gtgtcccgcc aagttcggtg gagactccca tcatgcttat
      541 aactttgaat tgtaataaag tcgccttctt ttggaagctt cataactaac caactttcta
      601 caatttataa gtttatcatt tactattgta ccataaaatt acctaaaatc tgtgaatttc
      661 acttggaaat attaaagata ttctctaaga gcgcttgcta tatccgaaaa atcgtagccc
      721 tttcgtgcta aaacttgagt taaacgctgc ttcagttcgt atccttcata ctttcgagca
      781 tacttagtat attgcttatc aagttccttg aagatgagtt cttgagtcgt ttcttggtca
      841 acttggctat ctaagtcgtc aaaggcaatt ttagcatcag aataagagaa gcccttgtta
      901 gtcaagttct ggataatctt atcttgcaag gcacgagctg gaagttttcc ctcatatttt
      961 ttcaatagtt tattagctac acgttgagca acttcagaaa aatcaaattc tttcaagttc
     1021 tcttctatag tagattttga aatccctttt tgagctagtt tctgagtcag cacataagga
     1081 cccttgtctc ctgaaagttg attggtattg atgatagcat aagcgtactg accatcatta
     1141 atccacttat cttctttaag attagcaata acttgagaaa cgatgttttt atcaatatcg
     1201 tattttttca gatattctct gacttctttt tcagtgcgtg ctttaaagga taagtggtag
     1261 agggccagat tcttaccata agaaaattga gcaaagtctt gaatctcttt caattcctct
     1321 tcgcttatca ccttatctct cgataacata aaacgaacaa ttgtatcttc ggtgatatag
     1381 catttgtcgc cattatcaag ctccatcaga tagagtcttt ttttcttttc aagttttgtg
     1441 attttcatag ctctattata actcaaaatg tgataagata ggggtatgaa tctgaaagtg
     1501 aaacaaaaaa taccattaaa aatcaagcgc atgggaatta acggtgaggg aatcggcttt
     1561 taccaaaaaa cattagtctt tgtaccagga gctctcaaag gcgaagatat ctattgtcag
     1621 attacttcta ttagacgcaa ctttgttgaa gcaaaattac tgaaggtcaa caagaagtct
     1681 aaatttcgaa ttgtgccatc ttgtactatt tataatgaat gcggaggctg ccaaatcatg
     1741 cacctgcatt atgataagca gctggagttc aagacggact tacttcatca agcgctgaaa
     1801 aaatttgctc ctgcaggata tgaaaattat gaaattcgtc caactattgg aatgcaggaa
     1861 ccaaaatatt acagagctaa gttacaattt cagactcgaa aatttaaaaa tcaggtcaag
     1921 gcgggcttat atgcacaaaa ctctcactat ttagtagagt tgaaagactg cctggtacaa
     1981 gataaggaaa cccaagtgat tgctaatcgc ttagcagaat tacttactta tcaccagatt
     2041 ccaatcacgg atgagagaaa agttctaggt gtccgtacta ttatggtccg acgcgcgaga
     2101 aagaccggac aggttcagat tattattgtt acaaaccgcc agcttaattt aactcaattg
     2161 gtaaaagagt tggttaaaga tttcccagaa gttgtgacag tagctgttaa tacaaataca
     2221 gctaaaacca gtgagatata tggtgaaaag acagagatta tctgggggca agagagtatt
     2281 caagaaggtg tactcaatta tgaattttca ctatcccctc gagcttttta tcaactaaat
     2341 cctgagcaaa cagaagtcct ctatagcgaa gcagtaaaag cgctggatgt tgataaagaa
     2401 gaccatttga ttgacgctta ttgtggagtt ggaacgattg gatttgcctt tgcaaagaaa
     2461 gtaaaaacac tcagaggtat ggatattatt ccagaagcta ttgaagatgc caagcgaaat
     2521 gctaaaagaa tgggatttga caatactcat tatgaagctg gaacggcaga agagattatt
     2581 cctcgttggt acaaggaagg ctaccgagca gatgctttga ttattgaccc accacgtaca
     2641 ggtctggatg ataagttatt agatactatt cttacttatg taccagaaaa aatggtttat
     2701 atttcttgta atgtttcgac cttggctcgt gatttggtac gcttagtaga agtctatgat
     2761 cttcattata tccagtcggt cgatatgttc ccacatacag ctcgaactga agctgttgta
     2821 aaattaataa aaaaagttta aaaaaggtgt tgacaaagtt tgaaaagact gtataatagt
     2881 aagagttgaa aataacaact caggtccgtt ggtcaagggg ttaagacacc gccttttcac
     2941 ggcggtaaca cgggttcgaa tcccgtacgg actatggtat gttgcgtcag gaccacttga
     3001 tgaaaaaaag tttaaaaaaa cttaaaaatc ttcaaaaaag tgttgacaag cgaaagcag
//
LOCUS       DAVAAL010000161         3025 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVAAL010000161 DAVAAL010000000
VERSION     DAVAAL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.163"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..1211
                     /locus_tag="VQM60_001920"
     CDS             <1..1211
                     /locus_tag="VQM60_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET1081742.1"
                     /translation="SDKQNLEAEIEEIKSNKNAIQERYQNLQEELAQARLLKTELQGQ
                     KRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDNLEKVDTELLSQQAEESKTQKTNL
                     QQGLIRQQFELDDIEGQLDDIASHLDQARQQNEEWIRKQTRAEAKKEKVSERLRHLQS
                     QLTDQYQISYTEALEKAHELENLNLAEQEVKDLEKAIRSLGPVNIEAIDRYEEVHNRL
                     DFLNSQRDDILSAKNLLLETITEMNDEVKERFKSTFEAIRESFKVTFKQMFGGGQADL
                     ILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKALSALALLFSIIRVKTIPFVILDE
                     VEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGTMAAADSIYGVTMQESGVSKIVSV
                     KLKDLESIEG"
     gene            1208..2002
                     /locus_tag="VQM60_001921"
     CDS             1208..2002
                     /locus_tag="VQM60_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET1081743.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSHDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            2002..2820
                     /locus_tag="VQM60_001922"
     CDS             2002..2820
                     /locus_tag="VQM60_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET1081744.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSNQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            2824..>3025
                     /locus_tag="VQM60_001923"
     CDS             2824..>3025
                     /locus_tag="VQM60_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET1081745.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEADEVP
                     QEEAEVEIVEQAVFQEEEIQDTV"
BASE COUNT          950 a          544 c          719 g          812 t
ORIGIN      
        1 ccagtgacaa gcaaaatctg gaagctgaga ttgaagagat taagtctaac aaaaatgcca
       61 tccaagaacg ctatcaaaac ttgcaggaag agctagcgca agctcgtttg cttaagacag
      121 aactgcaagg gcaaaaacgt tatgaagttg ctgatattga acgcttaggc aaggaattgg
      181 acaatcttga ttttgaacaa gaggaaatcc agcgccttct tcaagaaaag gttgataatc
      241 ttgagaaggt tgatacagaa ttgctcagtc aacaggcgga agaatccaaa actcagaaaa
      301 caaatctcca acaaggtttg attcgccagc agtttgagtt ggatgatata gaaggtcaac
      361 tggatgatat tgccagtcat ttggatcagg ctcgccagca gaatgaggag tggattcgca
      421 agcaaacacg tgctgaagcc aagaaagaaa aggtcagcga acgcttgcgc catctacaaa
      481 gtcaattaac agaccagtac cagattagct atactgaagc tctagaaaaa gcgcatgaat
      541 tggaaaacct caatctggca gagcaagaag ttaaggattt agagaaggct attcgctcac
      601 tgggtcctgt caatatagaa gctattgacc ggtacgaaga agttcacaac cgtctggact
      661 ttctaaatag tcagcgagat gatattttgt cagcgaaaaa tctgctcctt gaaaccatta
      721 cagagatgaa tgatgaggtc aaggaacgct ttaaatcaac ctttgaagct attcgtgagt
      781 cctttaaagt gaccttcaag cagatgtttg gcggaggtca ggcagacttg atattgactg
      841 agggcgacct tttaacagct ggtgtggaga tttctgttca acctccaggt aagaaaatcc
      901 agtcgcttaa cctcatgagt ggtggtgaaa aagctctatc ggctcttgcc ttgctcttct
      961 ccattattcg tgtcaagact attccatttg ttatcttgga tgaggtggaa gctgcgctgg
     1021 atgaagctaa tgttaaacgt tttggggatt acctcaaccg ctttgacaag gacagccagt
     1081 tcatcgtcgt aacccaccgt aagggaacca tggcagcggc tgattctatc tatggagtga
     1141 ccatgcaaga atcaggtgtc tcaaaaattg tttcagttaa gttaaaagat ttagaaagta
     1201 ttgaaggatg acaattaaac tagtagcaac ggatatggac ggaaccttcc tagatgggaa
     1261 tggacgcttt gatatggatc gtctcaagtc tctcttggct tcctacaagg aaaaagggat
     1321 ttactttgcg gtggcttcgg gtcggggatt tctgtctctg gaaaaattat ttgctgatgt
     1381 tcgtgatgac attattttca tcgcggaaaa tggcagtttg gtagagtatc aaggtcagga
     1441 cttgtatgaa gcgactatgt ctcatgactt ttatctggca acttttgaaa agctgaaaac
     1501 gtcaccttat gtagatatca ataaactgct cttgacgggt aagaagggtt catatgttct
     1561 agatacggtt gatgagacct atttgaaagt gagtcagcat tataatgaaa atatccaaaa
     1621 agtagcgagt ttggaagata tcacagatga cattttcaaa tttacaacca acttcacaga
     1681 agaaacgctg gaagatgggg aggcttgggt aaacgaaaac gttcctggtg ttaaggctat
     1741 gacaactggc tttgaatcta ttgatattgt tctggactat gtcgataagg gagtggccat
     1801 tgttgaatta gttaaaaaac ttggtatcac aatggatcag gtcatggctt ttggagacaa
     1861 tcttaatgac ttacatatga tgcaggttgt gggacatcct gtagctcctg aaaatgcacg
     1921 acctgaaatt ttagaattag caaagactgt gattggtcac cataaggacc agtcggttat
     1981 agcttatatg gagggcttat aatggcagat ataaaattaa tcgcattgga cttggacggg
     2041 accttgctga ctactgataa aaggctgacg gatcgtacca aggaaacctt gaaagctgcg
     2101 cgtgatcgtg gtatcaaggt cgtattgaca actggtcgtc ccttaaaagc tatggatttc
     2161 tttctccatg agctagggac tgacggtcag gaagatgagt ataccattac ttttaatggt
     2221 ggtttggttc agaaaaatac aggtgaaatc cttgataaga cagtcttttc atatgatgat
     2281 gtggcacgct tgtatgaaga aacagagaaa ttatcactgc ctcttgacgc tatctcagaa
     2341 ggaactgttt atcaaattca atcggaccaa gaaagtcttt atgccaaatt caatccagct
     2401 ttgacttttg ttccagttga ttttgaagac ctgtctaatc aaatgaccta caacaaatgc
     2461 gtgaccgcct ttgctcaaga acccttggat gcagccattc agaagatttc tccagaattg
     2521 tttgaccaat atgaaatctt taaatcacgt gaaatgttgc tagagtggtc accaaagaat
     2581 gttcataaag caacaggttt ggcaaaacta atcagccatc ttggaatcaa ccaaagccaa
     2641 gttatggcct gtggtgacga ggccaatgac ctttctatga ttgaatgggc aggtcttggt
     2701 gttgctatgc aaaacgctgt tcctgaagta aaggcagccg caaatgtagt aacgccgatg
     2761 accaacgatg aggaagctgt cgcctgggct atcgaagaat atgtgctaaa ggagaactaa
     2821 gatatgggat tgtttgaccg tctattcgga aaaaaagaag aacctaaaat cgaagaagtt
     2881 gtaaaagaag ctctggaaaa tcttgatttg tctgaagatg ttgatcctac cttcacagaa
     2941 gctgatgaag ttcctcagga agaagcagag gttgaaattg ttgaacaagc tgtgttccaa
     3001 gaggaggaaa tccaagacac agttg
//
LOCUS       DAVAAL010000162         3017 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVAAL010000162 DAVAAL010000000
VERSION     DAVAAL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.164"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            1..2966
                     /gene="addA"
                     /locus_tag="VQM60_001924"
                     /pseudo
     CDS             1..2966
                     /gene="addA"
                     /locus_tag="VQM60_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
BASE COUNT          938 a          663 c          641 g          775 t
ORIGIN      
        1 tgagaaagct gattttacca gtgaaaaaga aaaactgact gagcaaatca aacaagccct
       61 ctgggatttg gaaagctttt tccgttacca tctggataac gatgccaagg agtttgcaaa
      121 ggctgcctat ttagaaaatg ttcagttaat tctggatgaa attggctccc taaatcagga
      181 gttcgatagt caggcttatc aggcagtact tgcgcgtgtt gtcgccatct ctaaggagaa
      241 aaacggtcgt gctctgacta atgccagccg taaggctgat ttgaagcccc tggctgatgc
      301 ctacaacgaa gacccagttt actaaactag gacaattatc agaccagata gcgattctcg
      361 actatcaaga acgttatcat ggagacactt ggaaactagc taaaaccttc caatctttca
      421 tgagcgattt tgtagaggct tatcgtcaga gaaaacgaca ggaaaatgcc tttgaattcg
      481 ctgatatcag ccattacacc attgagattt tagaaaattt cccacaagtt cgtgagtctt
      541 atcaggagcg cttccatgaa gtcatggtcg atgagtatca ggataccaac catattcaag
      601 aacggatgct ggaattgttg tctaatggcc acaatcgctt tatggtggga gatatcaagc
      661 aatccatcta tcgtttccgt caggcagacc cgcagatttt caatgagaaa ttccaacgct
      721 atgcgcaaaa tccccaagaa ggcaagctca ttctcctcaa ggaaaatttc cgtagtagtt
      781 cagaagtgct gtcagcaacc aatgatgtct ttgaacgtct catggaccaa gaggtcggcg
      841 aaatcaacta tgataacaag caccagcttg tttttgccaa taccaaactg actcccaatc
      901 cagacaacaa ggcagaattt ctcctctacg acaaggacga tacaggtgag gaagaagaga
      961 gtcaaacaga aacgaaacta acaggcgaaa tgcgcttagt tatcaaggag attctgaaac
     1021 ttcatcaaga aaaaggtgtt acctttaagg aaattgccct tctgacctcc agccgcagtc
     1081 gtaatgacca gattctcctc gccctgtctg agtacgggat tcctgtcaaa actgacggag
     1141 agcaaaacaa ttatctccaa tccctagaag tgcaagtcat gctagacact cttcgtgtca
     1201 ttcacaatcc cctgcaagac tatgccttgg ttgcccttat gaagtctcca atgtttggtt
     1261 ttgatgagga tgagctagca cgtttgtccc ttcagaaagc agaggataaa gtccacgaaa
     1321 atctctatga gaaactggtc aatgcacaaa aaatggcaag tagtcaaaaa ggcttgattc
     1381 acacagctct agctgaaaaa ctaaagcaat tcatggatat cctagcttct tggcgcttgt
     1441 atgccaaaac ccactctctc tatgacttga tttggaagat ttacaacgac cgtttttatt
     1501 atgactatgt tggggctttg ccgaatggtc ctgctaggca ggccaatctc tatgccctag
     1561 cactgcgtgc tgatcaattt gaaaagagca atttcaaagg tttgtcgcgt tttattcgta
     1621 tgattgacca agtcttagaa gcccagcacg atttggcaag cgtggccgtc gcaccgccaa
     1681 aagatgcagt agagctcatg accatccaca agagtaaagg gctggagttt ccttacgtct
     1741 ttatcctcaa tatggatcaa gatttcaaca agcaagactc tatgtcagaa gtcattctca
     1801 gtcgtcagaa tggtcttggt gtcaaatata ttgccaagat ggagacaggg gcagtagaag
     1861 accactatcc taaaaccatc aaactctcca ttcctagtct gacctatagg cagaacgaag
     1921 aggaattaca gctagcaagc tattctgagc agatgcgttt gctgtatgtt gctatgacgc
     1981 gggctgagaa aaagctctat cttgtcggca agggttctcg tgaaaagctg gaagtcaagc
     2041 aatacccagc agccaaaaat gggaaactaa atagcaatac tagactgcaa gcacggaatt
     2101 tccaagattg gctttgggct atcagtaaag tgtttactaa ggacaagctc aactttagtt
     2161 atcgttttat tggcgaagat cagttgacca gagaagctat cggagagttg gaaaccaaga
     2221 gtcctctcca agatagctcc caagcagaca atcgtcagtc agataccatc aaagaagctc
     2281 tggaaatgct gaaggaggtg gaagtttata atactcttca ccgcgcagct attgaacttc
     2341 ctagtgttca aaccccaagt caaatcaaga aattctacga accagttatg gatatggaag
     2401 gtgtcgagat tgctggtcaa ggtcagtcag taggcaagaa aatcagcttc gatttgccag
     2461 atttttcaac caaagaaaag gtaactggag ctgagattgg tagtgctact cacgaactca
     2521 tgcagagaat tgacctcagc cagcaactaa cccttgctag cctaacagaa acactcaaac
     2581 aagttcaaac tagccaagct gtcagagaca agatcaatct tgataaaatt cttgctttct
     2641 ttgacacagt actcggtcag gaaattcttg ctaataccga ccatctctat cgcgagcaac
     2701 ctttctccat gctcaaacga gaccaaaaga gtcaggaaga ctttgttgtc cgtggtatcc
     2761 ttgatggcta tctgctttac gaaaacaaaa ttgttctgtt cgactacaag acagaccgct
     2821 atgatgaacc aagtcaactc gtagaccgct atcgtggtca gttagctcta tacgaagagg
     2881 ctttatcacg agcctattcg attgaaaata ttgaaaaata cttgatttta ctcggtaaag
     2941 acgaggttca agttgtaaaa gtataactta tactctttga aaatctcttc aaaccacgtc
     3001 agcttcgcct tgctgta
//
LOCUS       DAVAAL010000163         2816 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVAAL010000163 DAVAAL010000000
VERSION     DAVAAL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.165"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            60..929
                     /locus_tag="VQM60_001925"
     CDS             60..929
                     /locus_tag="VQM60_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET1081746.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILGISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            933..1889
                     /locus_tag="VQM60_001926"
     CDS             933..1889
                     /locus_tag="VQM60_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET1081747.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1889..2653
                     /locus_tag="VQM60_001927"
     CDS             1889..2653
                     /locus_tag="VQM60_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081748.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2653..>2816
                     /locus_tag="VQM60_001928"
     CDS             2653..>2816
                     /locus_tag="VQM60_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET1081749.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRP"
BASE COUNT          721 a          517 c          635 g          943 t
ORIGIN      
        1 agaacctatc aaaaagtgag ggaaacccct cggaattata aatagaaaga gtgaatctta
       61 tgctccaaca actcgtaaat ggtttgattc taggtagtgt ttacgcgctg ttagccctag
      121 gatataccat ggtttacgga attatcaagc tcatcaactt cgcccatggt gatatttata
      181 tgatgggagc ctttatcggt tatttcttga tcaattcttt ccaaatgaat ttctttgtag
      241 cgcttattgt agctatgcta gcgacagcta ttcttggtgt cgtgattgag tttcttgctt
      301 accgaccttt gcgccactct actcgtattg ctgttttgat tacggctatt ggggtttctt
      361 tcctattgga gtatggaatg gtctatctgg ttggtgccaa tacccgtgcc ttccctcaag
      421 cgattcaaac agttcgatat gatttgggac caattagctt aacaaatgtg cagttaatga
      481 ttttgggcat ttccttgatt ttgatgattt tgttacaagt cattgtccaa aagactaaga
      541 tggggaaagc catgcgtgca gtatcagtag atagcgacgc ggcgcaattg atggggatca
      601 atgtaaaccg tacgattagc tttaccttcg ctttgggttc tgctcttgcg ggtgcggctg
      661 gtgttctgat tgctctttat tataactctc ttgagccttt gatgggggtt actccaggtc
      721 ttaaatcttt cgttgccgca gtacttggtg gtatcggaat tattcctggt gctgctcttg
      781 gtggctttgt gattggtcta ttggaaacct ttgcgacagc ctttgggatg tcagatttcc
      841 gtgatgccat tgtttatgga atcttgttgt tgatcttgat tgtccgccca gctggtatcc
      901 ttggtaagaa tgtgaaagag aaggtgtaaa cgatgaagga aaatttaaaa gttaatattc
      961 tatggttact ccttttgtta gctggctata gcttgattag tgtactggtt tcagtcggag
     1021 tacttaatct attctatgta cagattttac aacaaattgg aattaatatt attttggctg
     1081 ttggtctcaa cttaatcgtt ggtttttcag gacaattttc acttggtcat gctggtttca
     1141 tggcgattgg tgcctatgca gcagctatta ttggttctaa atcaccaacc tacggtgcct
     1201 tctttggagc tatgcttgta ggggctttgc tttcaggagc agttgcctta cttgtcggca
     1261 ttccaacctt gcgcttgaag ggggactatc ttgcggtagc aactctaggt gtttctgaaa
     1321 ttatccgtat ctttatcatc aatggtggaa gccttacaaa tggtgcggca ggtatcttag
     1381 ggattcctaa ctttacaact tggcaaatgg tttacttctt tgtcgtgatt acaaccattg
     1441 caaccttgaa cttcttgcgt agcccaattg gtcgttcaac cctctctgtt cgtgaagatg
     1501 aaatcgctgc tgagtcagtt ggggttaata cgactaaaat taaaatcatc gcttttgtct
     1561 ttggtgccat tactgcaagt attgctgggt cacttcaggc aggatttatc gggtctgttg
     1621 taccgaaaga ttacaccttc atcaactcaa tcaacgtttt gattattgtt gtatttggtg
     1681 gactcggttc cattacaggt gcgattgttt cggctattgt tctgggaatt ttgaatatgc
     1741 ttctccaaga tgttgctagt gtgcgtatga ttatttacgc tttggccttg gtattggtaa
     1801 tgattttcag accaggtgga ctccttggaa cgtgggaact gagcctatca cgtttcttta
     1861 aaaaatctaa gaaggaggaa caaaactaat ggcattactt gaagtaaaac agttaaccaa
     1921 acattttggt ggtctaacag ctgttggaga tgtgactctt gaattgaacg aaggggaact
     1981 ggttggatta atcggtccaa acggagctgg gaaaaccacc cttttcaacc ttttgaccgg
     2041 tgtttatgaa ccaagcgagg gaacagtaac cctagatggt caccttttga atgggaaatc
     2101 accttataag attgcctctt tgggacttgg acgtactttc caaaatatcc gtctttttaa
     2161 agatttaaca gttttagaca atgttttgat tgcttttgga aaccatcaca aacagcatgt
     2221 ttttactagt ttcttacgct taccagcttt ttacaagagt gaaaaagaat taaaggctaa
     2281 agctttggaa ttgttgaaaa tctttgattt agatggtgat gcagagactc ttgctaaaaa
     2341 tctttcctac ggacaacaac gtcgtttgga aattgttcgt gcccttgcta cggaacctaa
     2401 aattctcttc ttagatgaac cagcagcagg tatgaaccca caggaaacag ccgaattgac
     2461 tgagttaatt cgtcgtatca aagatgaatt taagattaca atcatgttga ttgaacacga
     2521 tatgaatctg gtcatggaag taacagaacg tatctacgta cttgaatatg gccgtttaat
     2581 tgctcaagga actccagacg aaattaagac caataaacgc gttatcgaag cttatctagg
     2641 aggtgaagcc taatgtctat gttaaaagtt gaaaatcttt ctgtgcatta cggtatgatc
     2701 caagcagtcc gtgatgtaag ctttgaagtt aatgaaggag aagttgtttc ccttatcggt
     2761 gccaacggtg caggtaagac aactattctt cgcaccttgt caggtttggt tcgacc
//
LOCUS       DAVAAL010000164         2704 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVAAL010000164 DAVAAL010000000
VERSION     DAVAAL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.166"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..648
                     /locus_tag="VQM60_001929"
     CDS             <1..648
                     /locus_tag="VQM60_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET1081750.1"
                     /translation="MPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYT
                     LEDLLATVKYYVEHPNERPHSDNGFGNASDHVRKNKADQDSKPDEDKGHDEVSEPTHP
                     ESDEKENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEA
                     LLEKVTDPSIRQNAMETLTGLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            858..>2704
                     /gene="phtE"
                     /locus_tag="VQM60_001930"
     CDS             858..>2704
                     /gene="phtE"
                     /locus_tag="VQM60_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET1081751.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTL"
BASE COUNT          965 a          475 c          528 g          736 t
ORIGIN      
        1 atgccttaca atcttcaata tactgtagaa gtcaaaaacg gtagtttaat catacctcat
       61 tatgaccatt accataacat caaatttgag tggtttgacg aaggccttta tgaggcacct
      121 aaggggtata ctcttgagga tcttttggcg actgtcaagt actatgtcga acatccaaac
      181 gaacgtccgc attcagataa tggttttggt aacgctagcg accatgttcg taaaaataag
      241 gcagaccaag acagtaaacc tgatgaagat aagggacatg atgaagtaag tgagccaact
      301 caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc agataatctt
      361 tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac tacagatgag
      421 gctgaaattc ctcaagtaga gcattctgtt attaacgcta agatagcaga tgcggaggcc
      481 ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac attgactggt
      541 ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc agaagtagat
      601 agtctcttgg ctttgttaaa aaaaagtcaa ccggctccta tacagtagta aaatgaatgg
      661 agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa aaacgtaaca
      721 gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta aatggttaaa
      781 taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac ttcatcattt
      841 attgaaatga gggatttatg aaatttagta aaaaatatat agcagctgga tcagctgtta
      901 tcgtatcctt gagtctatgt gcctatgcac taaaccagca tcgttcgcag gaaaataagg
      961 acaataatcg tgtctcttat gtggatggca gccagtcaag tcagaaaagt gaaaacttga
     1021 caccagacca ggttagccag aaagaaggaa ttcaggctga gcaaattgta atcaaaatta
     1081 cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat gggaaagttc
     1141 cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat caacttaaag
     1201 acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat ggaaaatatt
     1261 atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat gaaatcaatc
     1321 gtcaaaaaca agaacatgtc aaagataatg agaaggttaa ctctaatgtt gctgtagcaa
     1381 ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct gatattatcg
     1441 aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac attcccaaaa
     1501 gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggctgga aaaaatatgc
     1561 aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa tctgtagcaa
     1621 aaggatcaac tagcaagcca gcaaataaat ctgaaaatct ccagagtctt ttgaaggaac
     1681 tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc tttgaccctg
     1741 ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac cattaccact
     1801 ttattcctta cagcaagctt tctgccttag aagaaaagat tgccagaatg gtgcctatca
     1861 gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg tctagtctag
     1921 gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct tcagcatctg
     1981 atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct tatattgtaa
     2041 gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa ccgactcttc
     2101 caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga acttcacatg
     2161 agaaacatga agaagatgga tacggatttg atgctaatcg tattatcgct gaagatgaat
     2221 caggttttgt catgagtcac ggagaccaca atcattattt cttcaagaag gacttgacag
     2281 aagagcaaat taaggctgcg caaaaacatt tagaggaagt taaaactagt cataatggat
     2341 tagattcttt gtcatctcat gaacaggatt atccaagtaa tgccaaagaa atgaaagatt
     2401 tagataaaaa aatcgaagaa aaaattgctg gcattatgaa acaatatggt gtcaaacgtg
     2461 aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga gatcaccatc
     2521 atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac agtaactatg
     2581 aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt tatactggag
     2641 aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat caaaacttta
     2701 ctct
//
LOCUS       DAVAAL010000165         2700 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVAAL010000165 DAVAAL010000000
VERSION     DAVAAL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.167"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            154..1647
                     /locus_tag="VQM60_001931"
     CDS             154..1647
                     /locus_tag="VQM60_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET1081752.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1660..2202
                     /locus_tag="VQM60_001932"
     CDS             1660..2202
                     /locus_tag="VQM60_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET1081753.1"
                     /translation="MKKVKLGEVCEILSGYAFKSSQFNDNKIGLPLIRIRDVERGFSD
                     TYFEGTYPEEYLIKNGDLLITMDGSFILKKWEGDLALLNQRVCKIKITDKSVDEGYIS
                     WLIPKFLKEIEDKTPFVTVKHLSVAKIKDISFVLPNKLEQKLIAKKLNTISQIYDFRK
                     IQSEKFNELVKSRYSEEVVA"
     gene            2199..>2700
                     /locus_tag="VQM60_001933"
     CDS             2199..>2700
                     /locus_tag="VQM60_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET1081754.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLREQQRIASELDLLSKLILRRQE
                     QLEELNL"
BASE COUNT          944 a          385 c          542 g          829 t
ORIGIN      
        1 cctacggcaa ggtgaagctg acgtggtttg aagagatttt cgaagagtat aaaatattca
       61 ggtgacgcat agatatagtt aattgaagct ttgtttgaaa tctgataaaa taatgatatt
      121 actaagtttt aaaaactaaa gaaaagggaa gatatgatta caggcgaatt aaaaaataaa
      181 atcgatcagc tgtgggaaat tctttggaca gaaggaaacg caaatccttt aacaaatatt
      241 gaacagttga cttatctctt atttatgaaa gatttggata gtgtcgagct tggacgtgaa
      301 agtgatgctg aatttctagg gattccttat gagggagttt ttccaaaaga taaacctgaa
      361 taccgttggt caacttttaa aaatatagga gatgctcagg aagtttatcg tttaatgact
      421 caggagattt ttccgtttat taaaaatctc aagggggata cagatgatac agccttttca
      481 cgatatatgc gagaagctat ttttcaaata aataaacctg ctacgcttca aaaggcaatt
      541 tctatcttag atgtttttcc aactagggga ttagatgtag attttgataa tgacaaacaa
      601 agtattactg atatcggaga tatctatgaa tatctgttat caaaattgtc gaccgcaggt
      661 aaaaatggac agttccgtac acctcgtcac atcatcgata tgatggttga gttgatgcaa
      721 ccgactatca aagatatcat ctcagatccc gctatgggtt ctgctggctt cttagtatct
      781 gctagccgtt acttaaagcg taagaaagat gaatgggaaa ccaatacaga taatatcaat
      841 cattttcata atcagatgtt tcatggaaat gatacggata cgactatgtt gagacttggg
      901 gcgatgaaca tgatgctaca tggagtagaa aatccacaaa tcagttacct tgactcgctg
      961 tctcaagata atgaagaagc cgataaatat actttggttt tagcaaatcc tccttttaag
     1021 ggctcacttg actacaattc aacctctaat gaccttcttg caaccgtaaa aaccaaaaaa
     1081 acagaattac tctttctttc tcttttcttg cgaactttaa aaccaggtgg acgagcagca
     1141 gttatcgtac ctgatggtgt cctttttggt tcgtctaaag ctcataaagg aattcgtcag
     1201 gaaattgtag agaatcataa gcttgatgct gtaatctcaa tgcctagtgg tgtgttcaag
     1261 ccttatgctg gagtttcaac tgccattctc atctttacaa aaactggtaa tggtggtact
     1321 gacaaagtct ggttttacga tatgaaagcg gatggtttaa gtttggatga taagcgacaa
     1381 ccgattagcg acaatgatat tccagatatt atcgaacgct ttcatcatct tgaaaaagaa
     1441 gcagaacgtc agagaacgga tcaatctttc tttgttccag ttgctgagat aaaggaaaat
     1501 gattatgatt tgtctatcaa taaatataaa gagattgagt atgaaaaagt tgagtatgaa
     1561 ccaacagaag tcatattaaa gaaaatcaat gatttagaaa aagaaattca agctggcttg
     1621 gctgaattgg aaaaattact caagtaggga ggtggctgta tgaaaaaagt gaagttgggg
     1681 gaagtttgtg agattttatc tggttacgct tttaaatcat cacaatttaa tgataacaaa
     1741 attggtctcc ccttaatacg aattcgagat gtagaaagag gtttttctga tacctacttt
     1801 gaaggaactt atcccgaaga atatcttatt aaaaatggag acttattgat tacaatggat
     1861 ggcagtttta tattgaaaaa atgggagggg gatttagcgc tacttaatca acgtgtctgt
     1921 aaaattaaaa ttacggataa aagtgtagat gaaggttata tatcttggtt gattcctaaa
     1981 tttttaaaag aaattgaaga taagacccct tttgttacag tgaaacatct ttcagtagct
     2041 aaaataaaag atatctcatt tgtattgcct aataaactag aacaaaagct aattgcaaaa
     2101 aaactaaata cgataagtca aatttatgat tttcgtaaaa ttcaatcaga aaaatttaac
     2161 gagctcgtca aatcccgata ttccgaggag gtggttgcat gaagaaagtg aaattagggc
     2221 aagtggctac ttttatcaat ggctatgctt ttaaacctca agattggtca tctgaaggga
     2281 aagagattat cagaatacag aatctgacta aaacttcaaa agggataaat tattattcgg
     2341 gtacgattga taaaaaatac attgttgaag ctggtgatat tcttatttcg tggtcgggta
     2401 cattaggggt ttttcagtgg tgtggaagat ccgcagtgtt aaatcagcac atttttaaag
     2461 tggtttttga taagatagat atagataaat cgtattttaa atatgttgtt gaaaaaggtt
     2521 tgcaagatgc tgtaaaacat actcatggtt ccacaatgaa acatttaact aaaaaatatt
     2581 ttgataatat tatggtttca tatacaaatt tgagagagca acaaagaatt gcaagtgagc
     2641 tggatttgtt aagcaagctg attttaagga gacaagaaca acttgaagag cttaatctac
//
LOCUS       DAVAAL010000166         2600 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVAAL010000166 DAVAAL010000000
VERSION     DAVAAL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.168"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..292
                     /locus_tag="VQM60_001934"
     CDS             <1..292
                     /locus_tag="VQM60_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET1081755.1"
                     /translation="IVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKT
                     LNDLKQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            366..1997
                     /gene="rlmD"
                     /locus_tag="VQM60_001935"
     CDS             366..1997
                     /gene="rlmD"
                     /locus_tag="VQM60_001935"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET1081756.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHDLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            2211..2444
                     /locus_tag="VQM60_001936"
     CDS             2211..2444
                     /locus_tag="VQM60_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HET1081757.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCN"
BASE COUNT          870 a          445 c          532 g          753 t
ORIGIN      
        1 gattgttgaa gataccttaa aaaattacaa acttgatcag gtcataacag gaaaccagat
       61 caatatcatc atcaattttg ccttgaatct ctcaaagagt gatatcctca gcaatgcaga
      121 tttcactaaa accctaaatg accttaaaca aagcatcgta tcacaagctg gcgacagttt
      181 taaaaatatc aaccttaact ttgatgcgga taaggcgcta gaggacggtg gtaacttctt
      241 aagctccctc tggcaagccc ttgtcaactt cttcaagagt tttggttctt aagaaatttc
      301 atggtataat ggatggtaac cgtaacgttg ccgtcttttt tgtcggccaa tagaaagaga
      361 aaagaatgtt aaagaaaaat gatattgtag aagttgaaat tgttgacttg acccatgaag
      421 gggcaggagt tgccaaggta gatggtttgg tcttttttgt agagaatgct ttaccgagtg
      481 aaaaaattct catgcgtgtc ctcaaggtca ataaaaagat tggctttgga aaagttgaaa
      541 aataccttgt ccagtcacca caccgtaatc aagatctaga tttggcttac ctgcgttcag
      601 gaatcgcgga tttaggacac ctttcttatc cagaacagct caagtttaaa accaagcaag
      661 tcaaggacag tctctacaag attgctggaa ttgcagatgt agaagttgct gaaacgcttg
      721 gtatggaaca tccagtcaag tatcgcaata aggcgcaggt gcccgttcgt cgagtgaatg
      781 gtgtcttgga aacaggattt ttccgtaaga attcgcacga cctcatgccc cttgaagatt
      841 tctttatcca ggatcctgtc attgaccaag tcgtagtagc tcttcgagac ctgctccgtc
      901 gttttgattt aaaaccttat gacgaaaagg aacagtctgg attgattcgg aatcttgtgg
      961 tgcgtcgtgg tcactattca ggacaaatca tggtcgtttt ggtgacaact cgtccaaaag
     1021 tttttcgtgt tgaccaattg attgaacaag ttatcaagca gttcccagag attgtgtctg
     1081 tcatgcaaaa tatcaacgac cagaatacca atgcgatttt tggtaaggag tggcgcactc
     1141 tttatggtca agactatatt acggaccaga tgttgggaaa tgacttccaa atcgctggcc
     1201 cagcctttta ccaagtcaat actgaaatgg cggagaaact ctatcaaaca gccattgact
     1261 ttgcagagtt aaaaaaagat gacgtggtta ttgatgctta ttctggtatt ggaaccattg
     1321 gtttatcagt cgccaagcat gtcaaagaag tctacggtgt tgaactgatt ccagaagcgg
     1381 ttgagaatag taaaaaaaat gctcagctga acaatatttc aaacgcccac tatgtctgtg
     1441 acacagctga aaatgctatg aagaattggc ttaaagatgg gattcaacca accgttatct
     1501 tggttgatcc tccacgcaag ggcttgacag aaagctttat caaagcaagc gcccaaacag
     1561 gagccgatcg catcgcctat atctcctgca atgtcgcaac catggcgcgt gatatcaaac
     1621 tctaccaaga attgggatat gaattgaaga aagtccagcc ggtggatcta tttcctcaaa
     1681 cgcatcacgt cgagacggta gcacttttgt ccaaactcga tgtcgataag cacataagtg
     1741 ttgaaattga gctggatgag atgaatttga caagtgcgga gagcaaagca acatatgctc
     1801 aaatcaaaga atatgtttgg aataaatttg aattaaaagt ttcgacatta tatattgcac
     1861 agataaaaaa gaaatgtgga atagaattac gagaacatta caacaagtct aaaaaggata
     1921 aacaaattat tccacagtgt acacctgaaa aagaagaagc catcatggat gctttgagac
     1981 acttcaaaat gatttaatag aaaagaatga cagtatatga ctttctgcat ttattacatt
     2041 cctacttggt ataggaacag ctattattcc tttcttgcaa ggtatcaatt agaaaatagg
     2101 ctcaatataa agattgatag gatcattttt atatttaaag gagtgttgaa atgattgata
     2161 aaggcaacaa aaaattttag gataaatttg ctaagttgta tgcccctttt atgaaaaaag
     2221 ataaagaggt ttatgataaa gtttgtgaat atcttagtcc tcatttgaat aaagatatgg
     2281 aggtgcttga acttgcttgt tggtttcgtg tcataatagt tatagaggca aatagttatg
     2341 taaatataag gagttcaaga cttctaccaa agtttaaaac tcaaaaaata aatagttggt
     2401 gtgctgctta tagtatccat tttaataatg gatattgtaa ttagcatacc aatgaattta
     2461 aagattcttt aaagagtctt attttgtgat gaaaatttaa tatgtaaatc tcagacgata
     2521 gaaattaaaa actctatcgt cttttttata ctcaaaatta ggaggtaaaa atggtaagga
     2581 taagaggtcc cacttaaaac
//
LOCUS       DAVAAL010000167         2536 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVAAL010000167 DAVAAL010000000
VERSION     DAVAAL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.169"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            171..731
                     /gene="efp"
                     /locus_tag="VQM60_001937"
     CDS             171..731
                     /gene="efp"
                     /locus_tag="VQM60_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET1081758.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            753..1142
                     /locus_tag="VQM60_001938"
     CDS             753..1142
                     /locus_tag="VQM60_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET1081759.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            1135..1557
                     /gene="nusB"
                     /locus_tag="VQM60_001939"
     CDS             1135..1557
                     /gene="nusB"
                     /locus_tag="VQM60_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET1081760.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(1607..1843)
                     /locus_tag="VQM60_001940"
     CDS             complement(1607..1843)
                     /locus_tag="VQM60_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET1081761.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(2325..2510)
                     /gene="briC"
                     /locus_tag="VQM60_001941"
     CDS             complement(2325..2510)
                     /gene="briC"
                     /locus_tag="VQM60_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET1081762.1"
                     /translation="MTGTETFTVISTEDLEQTSSGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
BASE COUNT          864 a          510 c          458 g          704 t
ORIGIN      
        1 cattgagtat gagaagaaat atagtggata ctctttaaca ttaaccactc cacctgacta
       61 gccaatattc tttttcgctc ctaccaactc ccaataacta ttttggcttt atttccagag
      121 tattttatgg taaaatgaag agtaataata tttattaaag aggtaaaaac atgattgaag
      181 caagtaaatt aaaagctggt atgacctttg aaacagctga cggcaaattg attcgcgttt
      241 tggaagctag tcaccacaaa ccaggtaaag gaaacacgat catgcgtatg aaattgcgtg
      301 atgtccgtac tggttctaca tttgacacaa gctaccgtcc agaggaaaaa tttgaacaag
      361 ctattatcga gactgtccca gctcaatact tgtacaaaat ggatgacaca gcatacttca
      421 tgaatacaga aacttatgac caatacgaaa tccctgtagt caatgttgaa aacgaattgc
      481 tttacatcct tgaaaactct gatgtgaaaa tccaattcta cggaactgaa gtgatcggtg
      541 tcaccgttcc tactactgtt gagttgacag ttgctgaaac tcaaccatct atcaaaggtg
      601 ctactgttac aggttctggt aaaccagcaa cgatggaaac tggacttgtc gtaaacgttc
      661 cagacttcat cgaagcagga caaaaactcg ttatcaacac tgcagaagga acttacgttt
      721 ctcgtgccta atctctagaa agaggtcatt ctatgggaat tgaagaacaa cttggcgaaa
      781 tcgttatcgc cccacgtgta cttgaaaaaa tcattgctat cgctactgca aaggtagagg
      841 gtgttcactc tttttcaaac agatcagtgt ctgataccct ttcaaaactt tcactcggcc
      901 gtggcattta tcttaaaaac gtggacgaag aactcacagc agatatctat ctctaccttg
      961 agtacggagt aaaagttcct aaggtagcgg ttgctatcca gaaagctgtc aaagatgccg
     1021 tccgtaatat ggctgatgta gaactcgctg ctatcaatat tcacgttgca ggtatcgtcc
     1081 cagataaaac accaaaacca gaattgaaag atctatttga cgaggacttc ctcaatgact
     1141 agtccactat tagaatctag acgccaactc cgtaaatgcg cttttcaagc tctcatgagc
     1201 cttgagttcg gtacggatgt cgaaactgct tgtcgtttcg cctatactca tgatcgtgaa
     1261 gatacggatg tacaacttcc agcctttttg atagacctcg tttctggtgt tcaagctaaa
     1321 aaggaagaac tagataagca aatcactcag catttaaaag caggttggac cattgaacgc
     1381 ttaacgctcg tggagagaaa cctccttcgc ttgggagtct ttgaaatcac ttcatttgac
     1441 actcctcagc tggttgctgt taatgaagct atcgagcttg caaaggactt ctccgatcaa
     1501 aaatctgccc gttttatcaa tggactgctc agccagtttg taacagaaga acaataaaaa
     1561 gaaaaagtgt ttgatagata tcaaacactt ttttctttgc ctcccactat ctaaaaaaat
     1621 gataatagat ataattgtaa acaaaaatcc agataggttt tgcatgattg agaaagttaa
     1681 aaaaactatg gcagagaatc gttaatctca gattgtcggt agaacgataa acaagggcaa
     1741 aaaagaaacc aatcagacta taatataata aactaattgg atctctgtga gatagtatca
     1801 aatggctaat cccaaagatg atagcagata ggataacatc caaataatac ttggactagg
     1861 gaaagaaggt gttcataaaa taccctctat caagagtctc ctcaaaaaca ggaccgatga
     1921 ttacaggcag gacaaaagat aagatagtcg ataaaaaggt tggttgtcca tttgaaagaa
     1981 ccacggtaaa atactcatca tgaatattcc tatgattaat caaatgagca tagcgtgccc
     2041 aaaaattacc gagaatctga taaaccacat aagttgcaaa taaggagaag acaaatgacc
     2101 agtcccagct ctttttcttt tttaacctcc aaattaatag aaaaggaaac ttcccactaa
     2161 tcccactgtt aaaataagag aatagacatc agctcctaac cctaaaatga tcgtcacata
     2221 caatccaatt gtttgtggta aataggtaga tagtaaaata ataagcaaaa atattccaaa
     2281 ttgtcttagt ttctttgtgt ttctcatcgt acttttttga aagattaccc tgttcggaag
     2341 ccgtacttcc aagcatctat ataagaatta agtgcccctt gcctcatata gggagcaaat
     2401 tctctataat ataaccatct actatatcca tcttcccaaa cagcaagacc acttgaagtt
     2461 tgctccaagt cctcagttga aataactgta aatgtttctg tacctgtcat tgcaagtacc
     2521 ttcttaaaat atagtg
//
LOCUS       DAVAAL010000168         2461 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVAAL010000168 DAVAAL010000000
VERSION     DAVAAL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.170"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(57..>2461)
                     /gene="carB"
                     /locus_tag="VQM60_001942"
     CDS             complement(57..>2461)
                     /gene="carB"
                     /locus_tag="VQM60_001942"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET1081763.1"
                     /translation="YENQMLRDASLSIIRALKIEGGCNVQLALDPNSFKYYVIEVNPR
                     VSRSSALASKATGYPIAKLAAKIAVGLTLDEVINPVTGSTYAMFEPALDYVVAKIPRF
                     PFDKFEKGERRLGTQMKATGEVMAIGRNIEESLLKACRSLEIGVHHNEIPELAAVSDD
                     ALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAELTKIDIFYLDKLLHIFEIEQELGAHP
                     QDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQLRLENKIVPVYKMVDTCAAEFDSETP
                     YFYSTYGWENESIRSDKESVLVLGSGPIRIGQGVEFDYATVHSVKAIQAAGYEAIIMN
                     SNPETVSTDFSVSDKLYFEPLTFEDVMNVIDLEQPKGVIVQFGGQTAINLAEPLAKAG
                     VTILGTQVADLDRAEDRDLFEQALKELDIPQPPGQTATNEEEAALAARKIGFPVLVRP
                     SYVLGGRAMEIVENEEDLRSYMRTAVKASPDHPVLVDSYIVGQECEVDAISDGKNVLI
                     PGIMEHIERAGVHSGDSMAVYPPQTLSQKVQETIADYTKRLAIGLHCLGMMNIQFVIK
                     DEKVYVIEVNPRASRTVPFLSKVTNIPMAQVATKLILGQSLSELGYQNGLYPESTRVH
                     IKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSDATLEKALYKAFEASYLHLPTFGNVVF
                     TIADDAKEEALNLARRFQNIGYGILATEGTAAFFASHGLQAQPVGKIGDDDKDIPSFV
                     RKGRIQAIINTVGTKRTADEDGEQIRRSAIEHGVPLFTALDTANAMLKVLESRSFVTE
                     AI"
BASE COUNT          667 a          539 c          548 g          707 t
ORIGIN      
        1 ttttagtatt cctgtgtact gttctaaatt cagtctgcta tatcttattt ttctatttaa
       61 atcgcttctg taacaaagct acgactttca agtaccttaa gcatggcatt agctgtatct
      121 agcgctgtga agaggggcac cccgtgttca atggctgaac gacgaatttg ctcaccatct
      181 tcgtcagcag ttcgttttgt tcctactgtg ttaatgatag cttgaattct tcctttgcgt
      241 acaaaacttg ggatatcctt atcgtcatca ccaatcttac caacaggttg ggcttgcaag
      301 ccatgactag caaagaaggc tgctgtccct tctgtcgcaa ggattccata accaatgttt
      361 tggaaacgac gagccaagtt caaggcttct tctttggcat catcagcgat ggtaaagacg
      421 acattaccaa aagttggcaa gtgtagataa gaagcttcaa aggctttata gagagctttt
      481 tccaaagtag catcagaacc cataacttca cctgttgact tcatttcagg accgagcaag
      541 ctgtctacct tagctagttt ggtaaaggag aagacaggtg ccttgatatg aacacgggtg
      601 ctttcagggt aaagtccatt ttggtagcca agttctgata aactttgacc aagaatgagc
      661 ttggtcgcta cttgagccat aggaatattg gttaccttag ataggaatgg aacggtacgg
      721 ctggcacgtg gattgacctc aataacgtag actttttcat ccttgataac aaactggatg
      781 ttcatcattc caaggcagtg aagaccgatt gctaagcgtt tggtgtagtc tgcgatggtc
      841 tcctgaacct tttgcgacaa ggtttgtggt gggtaaacag ccattgagtc acctgagtgg
      901 acaccagcac gttcgatatg ctccatgata ccaggaatga gtacattttt accatctgaa
      961 atggcatcaa cttcgcactc ttgcccaacg atataagagt cgacaagaac tggatggtct
     1021 ggactagcct taacagcagt tcgcatgtaa gaacgaaggt cttcttcgtt ttcaacgatt
     1081 tccatggcac gtccaccaag tacataagat gggcggacaa gaactgggaa gccaatctta
     1141 cgagctgcaa gagctgcttc ttcttcattg gtagccgttt gtcctggtgg ctgtggaata
     1201 tccaattctt tgagagcttg ctcgaagagg tcacggtctt cggcacggtc taggtcagca
     1261 acctgtgtac caaggatggt cacacctgct tttgccaatg gctccgcaag gttgatggct
     1321 gtttgaccac cgaactgaac gataactccc tttggttgtt ccaagtcaat gacgttcata
     1381 acatcttcga atgtcaatgg ctcaaagtaa agcttatctg atacagagaa gtctgttgaa
     1441 acggtctctg ggtttgagtt catgatgata gcttcataac cagctgcctg gatagcctta
     1501 acagagtgaa cggttgcgta gtcaaactca accccttgac cgatacggat tggacctgaa
     1561 cctaggacaa gtacagattc tttatcagat ctgatagatt cattttccca accataggtt
     1621 gaatagaaat atggcgtttc ggagtcgaac tctgccgcac aagtgtctac catcttataa
     1681 actggaacaa tcttgttttc caagcgaagt tggcgaactt tatcatcagt cgttccccag
     1741 agttcagcaa tcttacggtc tgaaaaacca ttaagtttgg ctgttttcaa aacttctaga
     1801 tcttgtggat gagcacccaa ttcttgctca atttcaaaga tatgcaagag tttatcaaga
     1861 tagaagatat caatttttgt aagctctgca atttcttcag gtgtgtagcc acgacgaatg
     1921 gcttctgata cgtagaagag acggtcatct tgggctttga caaccttttc aatcaaggca
     1981 tcatcagaaa ctgctgcaag ttcaggtatt tcattgtggt gcaccccaat ttcaagggag
     2041 cggcaggcct tgagaagaga ttcctcgatg ttacgaccga ttgccatgac ttctccagtc
     2101 gccttcattt gtgtaccgag acggcgttca cccttttcaa acttgtcaaa tgggaaacgt
     2161 ggaatcttag caactacgta gtcaagggct ggttcaaaca tggcataggt tgaacctgta
     2221 actgggttta taacctcatc caaggtcaaa cctactgcaa tcttggcagc caacttagca
     2281 atcggatatc ctgtcgcttt agaagcaagg gctgacgaac gtgatacacg agggtttact
     2341 tcgataacat aatacttgaa gctgttagga tcaagagcta gctgaacatt acatccacct
     2401 tcaatcttga gggcacgaat aatgctcaag ctcgcatcac gaagcatttg gttttcatag
     2461 t
//
LOCUS       DAVAAL010000169         2434 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVAAL010000169 DAVAAL010000000
VERSION     DAVAAL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.171"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            11..1189
                     /locus_tag="VQM60_001943"
     CDS             11..1189
                     /locus_tag="VQM60_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081764.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            1398..>2434
                     /locus_tag="VQM60_001944"
     CDS             1398..>2434
                     /locus_tag="VQM60_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET1081765.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLVELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTKLINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTV"
BASE COUNT          875 a          429 c          485 g          645 t
ORIGIN      
        1 aggagacctc atgacacttc cagttagaaa atccctgcac gatgcagttt tacaggcttc
       61 gaaagcggat acttgggaac aagctaccaa ggaatggaat gaagtttcct tgatttttaa
      121 cggtatcggc cgtagcaatt gtgtctgtgg aaatgccatc aaatacgctt atgaactctt
      181 taacggtgtc acaggccaac gcctctttcc aattggtagc gactgtgttc gtcattttca
      241 tcgattgtcc cttgaccagc aattggaaga ggaagaaaaa ctgctcagaa aggttgaaaa
      301 tctaaccaga aaagctcaga aaaaggaaaa aatcaaggtc aataaaagtg actttgacga
      361 gcgacttcta aaatggctct gggaaaaagg tgttttcaaa cccaatcgtg gcaatcaatt
      421 tgcgcctgag agagactacc agcttttcct agaagtcttt cagggaggaa gttggaccaa
      481 ggcggaacca aagaaaaagg ctcggatgga agaagtcctt gaaaagtgta tcaaaccctt
      541 tttacttggg aaatcggatg accaactcta ccttgtcaag ctaggcaagg agaaaattga
      601 ctacgaacaa gaactccgta tccaggcaga gaaagaacgc aagaagaggg ataaaatcgc
      661 caagcaatat gcagacaatc tggttctagc catgggaccc gcagagcgtg cttatcaaga
      721 ttactttggc tttacagaga ccctgactca agaagaacgc aagtgggaaa aaattctttt
      781 tggtaaaaat agagcagaac gggctatcaa ggccaaacaa taccaaaaag agctggaaaa
      841 agaccaacga attgccagtc aggatccaat tgaaagaaaa cagaagcaga cttggctcct
      901 caattcctat tttcgtgagc ttcctgaaga aaaagctcga ttttctaggc tcttattaga
      961 atatcgaaaa agtggtgaag tacccttttc aactgaatac ctgtccgatc atctcatcga
     1021 ctttttctat aaaatgaaag cctttgaatt tgaaatcgca ccagaacaag tccgagattt
     1081 tctaaaaaaa agcctgcagg aagaccatcg ctcatcagca caaggaagct ggatagaagg
     1141 aattctcctc aactgcctca aaccattttt agaacgatta gtgatatgag taacacctac
     1201 cgtgtaaatt tcatggtagg tgttattgta cccccaaata atataaaaca aagtatgaaa
     1261 gcgcatctca attttaagac ttttttgtaa attaagagta taattaaggt atactgcctt
     1321 ttctatagat aatagataat acactaatat aataaaggtc tttgtaacat atataaattt
     1381 tatttcaagg aggaataatg gaaaagtatt ttggtgaaaa acaagagcgt ttttcattta
     1441 gaaaattatc agtaggactt gtatctgcaa cgatttcaag tttatttttt atgtctgtat
     1501 tagctagttc atctgtggat gctcaagaaa ctgcgggagt tcactataaa tatgtggcag
     1561 attcagagct atcatcagaa gaaaagaagc agcttgtcta tgatattccg acatacgtgg
     1621 agaatgatga tgaaacttat tatcttgttt ataagttaaa ttctcaaaat caactggtgg
     1681 aattaccaaa tactggaagc aagaatgaga ggcaagccct agttgctggt gctagcttag
     1741 ctgctctggg aattttaatt tttgctgttt ccaagaaaaa ggttaagaat aaaacggtat
     1801 tacatttagt attggttgcg ggaatgggaa atggtgtctt agtttcagtc catgctttag
     1861 aaaatcatct tttgctaaat tacaatacgg actatgaatt gacctctgga gaaaaattac
     1921 ctcttcctaa agagatttca ggttacactt atattggata tatcaaagag ggaaaaacga
     1981 cttctgattt tgaagtaagt aatcaagaaa aatcagcagc cactcctaca aaacaacaaa
     2041 aggtggatta taatgttaca ccaaattttg tagaccatcc atcaacagta caagctattc
     2101 aggaacaaac acctgtttct tcaactaagc cgacagaagt tcaagtagtt gaaaaacctt
     2161 tctctactaa attaatcaat ccaagaaaag aagagaaaca atcttcagat tctcaagaac
     2221 aattagccga acataagaat ctagaaacga agaaagagga gaagatttct ccaaaggaaa
     2281 agactggggt aaatacatta aatccacagg atgaagtttt atcaggtcaa ttgaacaaac
     2341 ctgaactctt atatcgtgag gaaactatag agacaaaaat agattttcaa gaagaaattc
     2401 aagaaaatcc tgatttagct gaaggaactg taag
//
LOCUS       DAVAAL010000170         2428 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVAAL010000170 DAVAAL010000000
VERSION     DAVAAL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.172"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(1..2234)
                     /gene="pitA"
                     /locus_tag="VQM60_001945"
                     /pseudo
     CDS             complement(1..2234)
                     /gene="pitA"
                     /locus_tag="VQM60_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516426.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus tip adhesin PitA"
BASE COUNT          682 a          480 c          441 g          825 t
ORIGIN      
        1 ggtttatttt gcgcatcctt tacgttaatg gtaatcttaa aggattgcag aagattagca
       61 aaagcacctg taactttttt tgtgactgaa agtgtcggaa gtctgtggtt ggtgacgatt
      121 gtttgaacat cctctgacaa agtcccctct tgtttttcat aggtgacatg atatcccgtt
      181 ctagagttct ctacttcttc caccttatag tgggtatcac gaggcagata actgagggca
      241 gtttcctgac tatttttgag agaaatctgt gctttcccgt tagtcactgt tgtattccca
      301 aacttgccgt taatcggctt gccatcctta tctgaaaggg taacattaat tgtgaaagat
      361 ttattaggtg attgttcacc ttctaggatt ttctttacgg tgagacttgg aagctgtcgg
      421 taaataactt tgaaggatgg tttctctttc gtacctgtct tttgaacttc gactgtgtat
      481 cccggagcgt ctggtgcttt cagttcgtag tcatatccct tagctacctt tgcaaaacta
      541 gtattttcag taaaactatt cgtttgaact ggactagtga cctttcgata ggaatccttt
      601 atacttccat ctttcttttg gtttaagttt aattcgacac taggtggacg atttgctgat
      661 ggatttgatt ttccatccac acctttccac tcaatctcta caggaaccgt caatggatct
      721 gacggcttga aagtgggctt ctcagaatag tcttttattt tggtacgtga ggtcccaata
      781 ccataggaat aggcaacttt tgcccccttg ttggagtaga gtttagtagc atcggcttct
      841 tcggcatcat cactagtaag tgtcttatca cccgcaatcg catcaaatgc ctcttggcta
      901 gaggtgacag taaacttgag aacgtacttg gcgtcatcct ccaaagtgta actcggatta
      961 aacttggctg ttacttccac taatccctgt tcgttcttct tagtttcaat cgtaacttga
     1021 ttgtcattca gttcttgctc gttaccatcc ttgattttga cgacacgaag gtgtgaagca
     1081 tcaccaggta acagctgcac gtacttggac aagacatctg atatagttac atggtgtacg
     1141 cctagaggta gaattttatc tgtgatttct ttgaattttt gttgcaagtc attttcatta
     1201 ttggcagaaa atatttcgtt ggggattcca gaattataac cactgatata tcctttaaga
     1261 gatgtgatac tatcgtttga acctatatat ctgaatttga ttgaataaaa gccatctatt
     1321 tttggagcta ttaaactaga tatattcagc attcctctct ctaaaccaga ggtaaaccaa
     1381 tatggagtat caatcatacg tcccacattt ggattagaat aatagttata tattgttctc
     1441 ccactattag actcgtaata catattagct tcgccatctg acagcagaat aactactttt
     1501 tttgccccag cccttgcact ttgcaacaat ctttgagctt cagttaaccc agcatttata
     1561 tttgtcccag tgccaatgcc cgcgtccata ccataagaat gactagagtc agcaattgtt
     1621 aacgaactaa caatattttt tgagctatta gaatccgtgc tccaagatac taaagtttta
     1681 gcatcatcat atgaagagat atttgaatac caaccagctc aatttggcca agttccatat
     1741 tgataactca gataatattc attataataa cggccattat actgattatc aatctttccg
     1801 gcaaaaccaa ctatagacag acggttttgg gaattagaaa gaattgtatc aatgagcccc
     1861 tgacgaccat tcacaccttt cagtgtatgt tttagtgctt caatcctact aatgtatcgt
     1921 ctactagagt aagaccacac atctcgctct tccatactcc ctgaaaaatc ggcaaccaag
     1981 actacatcca agggttctga ctgggtctcc gtctgggtct ccgttcccag tttagatgtg
     2041 atatccagtg acaactcata ctggtcttct tgccctgata tcggagtaat cgttttgtgc
     2101 agagttgtct gaggttcagt tgttgaatct gcacgtaaag aattaattgg tgcacacaaa
     2161 aaaagaatag cgagaaagat aactaaacta ctggtaaaat attgaatctt cttaaaatca
     2221 tattgaacgt tcatgctatc tcccttgcta gaatatgttt taatctgata ctatataaat
     2281 ctgagacttc cataagataa ttgtataata atattaactt cgtgtcaatt aatgtgtttg
     2341 aaaacatgtt attataaatt acaaaaaagt atgattcttc tttgtttttc aactttttct
     2401 gtggtaaaat ggaaaaaata gtaaaaaa
//
LOCUS       DAVAAL010000171         2397 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVAAL010000171 DAVAAL010000000
VERSION     DAVAAL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.173"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            119..1606
                     /locus_tag="VQM60_001946"
     CDS             119..1606
                     /locus_tag="VQM60_001946"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET1081766.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRFE"
     gene            complement(1650..>2397)
                     /gene="ftsY"
                     /locus_tag="VQM60_001947"
     CDS             complement(1650..>2397)
                     /gene="ftsY"
                     /locus_tag="VQM60_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET1081767.1"
                     /translation="LRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQDNL
                     TVMLFVGVNGVGKTTSIGKLAHRYKRTGKKVMLVAADTFRTGAVAQLAEWGRRVDVPV
                     VTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKRVVP
                     EAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREELNIP
                     VKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
BASE COUNT          704 a          533 c          459 g          701 t
ORIGIN      
        1 ttctacctcg tattggaatt tttgattttt cggaaaatta tgttagaatt aagtttatga
       61 aatgaggttt cctcatacct agcaagacta ggaataaaaa tagaaattag gtagctagat
      121 gtcatctaag gttattgtta caattttcgg tgcgagtgga gacctggcta aacgcaagct
      181 ctacccttcc ctttttagac tatataaatc cggcaatctt tccaagcact ttgccgttat
      241 tggaactgcc cgtagacctt ggagtaagga atattttgaa tctgtagttg tcgagtccat
      301 ccttgatttg gcagatagta ccgagcaagc ccaagaattt gctagccact tctactatca
      361 aagccatgat gtcaatgatt cggaacatta tattgctttg cgtcaattac aagctgagct
      421 taatgaaaaa taccaagctg aacacaataa gctcttcttc ttgtctatgg cacctcagtt
      481 ctttggaacc attgccaaac acctcaaatc tgaaaacatt gtcgatggca aaggttttga
      541 gcgcttgatc gttgaaaaac catttggtac agattacgca actgcaagca agttgaatga
      601 cgaactccta gcaacatttg acgaagaaca aattttccgt atcgaccatt atcttggtaa
      661 ggaaatgatc caaagcatct ttgcagttcg ctttgcaaac ttgatttttg aaaacgtttg
      721 gaacaaggat tttatcgaca atgttcaaat tacctttgcg gagcgcttgg gtgtagaaga
      781 acgtggtggc tactatgacc aatccggtgc cctccgtgac atggtccaaa accacactct
      841 acaacttctt tcgctcctcg ctatggacaa accagcaagc ttcacaaaag acgagattcg
      901 tgctgaaaag attaaggtct ttaaaaacct ctatcatcca actgatgaag aactcaaaga
      961 acactttatc cgtgggcaat accgctctgg taagattgat ggcatgaaat acatctctta
     1021 tcgtagcgaa ccaaatgtga atccagaatc aacaactgaa acctttacat ctggtgcctt
     1081 ctttgtagac agcgatcgat tccgtggtgt tcctttcttt ttccgtacag gtaaacgact
     1141 gactgaaaaa ggaactcatg tcaacatcgt ctttaaacaa atggattcta tctttggaga
     1201 accacttgct ccaaatattt tgaccatcta tattcaacca acagaaggct tctctcttag
     1261 cctaaatggg aagcaagtag gagaagaatt taacttggct cctaactcac ttgattatcg
     1321 tacagacgcg actgcaactg gtgcttctcc agaaccatac gagaaattga tttatgatgt
     1381 cctaaataac aactcaacca actttagcca ctgggatgaa gttggtgcgt catggaagtt
     1441 gattgatcgt attgaaaagc tctgggctga aaatggtgcc ccacttcatg actataaagc
     1501 tggaagcatg ggacctcaag ccagctttga cctacttgaa aaattcggtg ccaaatggac
     1561 ttggcaacca gatatcgcct atcgtcaaga tggtcgtttc gaataaaaaa atcctgcaag
     1621 tttgtacctt gcaggatttt tgcttctgat tagattaagc cttccaagag acctttcata
     1681 aagttttctg agttaaactc tccaatatca tcgatttttt caccaaaacc aatcaatttt
     1741 acaggaatat tgagttcttc acgaatggct agaaccacac ctcctcgagc agttccatca
     1801 atcttagtca aaacaattcc cgttaaaggt gtgattttcg aaaattcttt ggcctgtact
     1861 agggcatttt gacctgttga tgcatcaagt gccaagaagg tttcatgtgg tgcttctggc
     1921 acaacacgtt tgataatacg accaatcttt tccaactcag ccataaggtt atccttattt
     1981 tgcagacgac cagcagtatc aatcatgaga atatcgatac cttcagccac ggcacgttcc
     2041 ataccatcaa agaccacgct ggctggatca gctttttcag gtccagttac tactggaaca
     2101 tctactcgtc ggccccattc agctagctga gctactgcac ccgtacggaa ggtatctgct
     2161 gcaaccagca tgaccttctt accagttcgt ttgtagcggt gggctagttt tccaatagaa
     2221 gttgttttcc caacaccatt cacaccaaca aagagcataa ctgtcaagtt atcttggaag
     2281 tggatgcttt catcgtagct accatccttt tcataaagct caaccaattt ctcaatgatg
     2341 acacgacgaa gtgcatcagg tttcttggca ttttcaagct tggcttcgta acgtagt
//
LOCUS       DAVAAL010000172         2373 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVAAL010000172 DAVAAL010000000
VERSION     DAVAAL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.174"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..591
                     /locus_tag="VQM60_001948"
     CDS             <1..591
                     /locus_tag="VQM60_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET1081768.1"
                     /translation="FGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTKN
                     VTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRIN
                     SGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQN
                     EFADFVVQIDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            651..887
                     /locus_tag="VQM60_001949"
     CDS             651..887
                     /locus_tag="VQM60_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HET1081769.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            884..1297
                     /locus_tag="VQM60_001950"
     CDS             884..1297
                     /locus_tag="VQM60_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET1081770.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1415..>2373
                     /locus_tag="VQM60_001951"
     CDS             1415..>2373
                     /locus_tag="VQM60_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET1081771.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKF"
BASE COUNT          876 a          352 c          448 g          697 t
ORIGIN      
        1 tttggggaaa ataaaatatt tgaaagcatt gataacttat ttgatattat agatggtgat
       61 aggggcaaaa attatcctaa atcagatgag ttgtttagtg aggagtactg tttattttta
      121 aatacaaaga atgttactaa aaacggattt tcattcgata caaagcaatt tatcactaaa
      181 acaaaggata aattacttcg aaaaggcaaa cttgagcgtt atgatatagt cttgacaaca
      241 agaggtactg ttggaaatgt agcgtactac gatgaattaa taaaatataa acatttacgt
      301 ataaattcag gtatggtaat attacgtccc aagacaccaa atctaaatca gaaatttatt
      361 atccatgttt taaggaataa taattatagt cgagtgatat caggaagtgc tcagcctcag
      421 ttaccaatta caaaattaaa aaaaatactt ctccccctcc ccccactagc cctccaaaat
      481 gagttcgcag actttgtagt tcagatcgac aaatcacaat tggcaatcca aaaatctctg
      541 gaagaacttg aaactttgaa gaaatctctg atgcaggagt attttggctg atattctgcc
      601 attgtaatta cggtaatgat ttgttataat acttcaaagg aggaaatcag atggtagtaa
      661 aaacaagaaa acaaggaaat tcaatcacca ttacgattcc aagtgaattt aatattccaa
      721 gtggtgttaa atacgaagcg aaattgttac caagtggtga gattatcttt actcctgaag
      781 aattggggca gcaggtttct tatgtatctg atgatgcctt tgacttaaat ttagataaaa
      841 tatttgacga atacgacgat gttttcaaag ctttggtgga aaaatgacaa tctatctgac
      901 agaaaagcaa attgaaaaaa taaatgcttt agcaattcaa cggtattctc caaatgagaa
      961 aattcaaaca gttagttctt ctgccttaaa tatgattgtg aacttaccag aacaatttgt
     1021 ctttgggaag tctctttatc caacaatttt tgataaagca acgatactat ttgtccaatt
     1081 gataaagaag catgtttttg ctaatgctaa taaaagaact gctttcttcg ttttggtcaa
     1141 atttttacaa ttaaacggct atcgtttttc tgtaacggta gaagaagcag taaaaatgtg
     1201 tgtaaccatc gcagtagaag ctttaactga tgaaaaaatg acaagctact ccaaatgggt
     1261 ttctgaacat tctgttagag aaaaggtcaa aaagtaacct agtatgctgg atttgaatga
     1321 gcacaagaaa ataaatgaac agacaatatt agaattctgt aatgcagaaa ctgatattgt
     1381 ctctttttat tgatgaataa gaaagtgaga aattatggaa tcaaaagtta caattatcat
     1441 gcaagaaatg ttacctcttt taaataatga acaattacta gcgttgagag agagtttaga
     1501 acatcatcta gtagacggaa aaaagcagca gaagtattcg aataataacc tgttgcaact
     1561 atttattacc gccaagcagg tagagggctg tagctcaaaa acaattcgtt attaccagag
     1621 gacgattgaa aacttgttta atgctattaa agagtctgtg acacaactca caacagatga
     1681 tttaaggagt tatttagcaa attaccagtc tgaaaaggat tgtagtaagg caaatttaga
     1741 caatattagg cgtatattgt cttctttttt tgcttggctt gagcaagagg aatatatcat
     1801 taaaaatccc attcgacgga taaagaaaat taagactgag caaaatgtga aggaaactta
     1861 tactgatgaa catttggaaa ttatgcgtga taactgtgaa aatttgagag atttggcaat
     1921 aatagaccta ctagcatcga caggtatgcg tgtaggggag cttgtacagt tgaatcgttc
     1981 agatattgat tttgaaaaca gagagtgtgt tgtctttggt aaaggaaaga aggagagacc
     2041 agtatatttt gatgctcgga cgaaaattca tttaagaaat tatcttaacg acagaaaaga
     2101 tagtcaccct gctctttttg taacgctagt tggaaaagtc cagagacttg gaattgctgg
     2161 tgtagagatt cgcttaagaa agttaggaga caaactcggc atacaaaagg ttcacccaca
     2221 taagttcaga agaactttag cgactaaggc aattgataaa ggtatgccta tcgaacaagt
     2281 ccaaaaactg ctaggtcata gcaagattga cacaaccctg gcctatgcca tggtcaatca
     2341 aaataatgtc aagcattcac accaaaaatt cat
//
LOCUS       DAVAAL010000173         2358 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVAAL010000173 DAVAAL010000000
VERSION     DAVAAL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.175"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(266..491)
                     /locus_tag="VQM60_001952"
                     /pseudo
     CDS             complement(266..491)
                     /locus_tag="VQM60_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1339..2211
                     /locus_tag="VQM60_001953"
     CDS             1339..2211
                     /locus_tag="VQM60_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET1081772.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
BASE COUNT          696 a          412 c          467 g          783 t
ORIGIN      
        1 attcttctca gctagtgtac taaggagtgt gtgcctgaaa atatgggaac taaggggctg
       61 gtttatcggt ttctctagtt tagtatttgc cttttgcaaa gtgatcttaa atgcctttct
      121 ctaaatttac atatcactat tgtttaacaa aatctaatct attttaggtc acttattctt
      181 tttttgaaat gtagaatgaa ctttttcaaa gtttttcgaa tcttttaaaa tctgtttgtt
      241 ttatatcgcc attctccccc cttttttaat tctccctata tagcctgaca actttcccga
      301 tggtacgaat atggttgctt tcgtctaggt ggatgtcggg gtattcggga ttgagttttt
      361 ttgaggtagc cttggcggag tttcttgaca tagttagcgc cgtctacttg gaagatgccg
      421 atggtattat agtcaatctg tggggtattc ttgataaata ggtagtcgct gtttcttatc
      481 tttgcctcca tggacttgct gacgacataa gcgattgggt cgtagtcgtc tgggataatg
      541 gaaactccat aactaaatcg tcgtcctgca tcgagcggct acctgcagag ataaactacc
      601 taacacgaga gtaagtagtc tgtctgtagt cgtccagtct gatgattttt acgatccttc
      661 gtttttctgc ttatacagtt gcctctcggc ataggttagg actttacctt gtctgggtgg
      721 tgcccgttgg tcgtagatag attggatatc gctaggagaa tccttttgaa ctggaggaaa
      781 gagggcatca atcaagctac tgaatacttt aactaagtca aatatagtat ttttcttagt
      841 agacctaacc cttttttcat aatttctaat ggtgttttta cttataccta tcttagtacc
      901 caattcttat tgagtccaac cattactagt ctatattgtt ttatagttga ttgagtttgg
      961 aatagtacgc tgtagctgct aaaacatttc taggaattaa tttgcctttc ctaatagagt
     1021 tgttcatatc ttatttcaat ctattatatt tttcgcctct aacaatcgca atctcttctt
     1081 tatctacgaa cgaaatcgct ttctattttt gtaagtaaag catcacacga aatccacgaa
     1141 aatgaaaacc tttgttgtgt tttcgtaaaa aatttgttga cagagcacga aacgcctact
     1201 ataattaaat caagtttaag gaaataacaa aaaaacaaat cggagggaaa gacgatgaac
     1261 acattaaatg agaaagtaat caatatctgt aaagcagtag ttaaagaaac tttaatccaa
     1321 gacatttagg agaaagaaat gaagaaatca agaaaattag ccacattagg aatctgttca
     1381 gccttgtttt taggcttggc tgcttgtcaa caacaacatg ctacttctga gggtacgaat
     1441 caaaggcaaa gcagttcagc gaaagttcca tggaaagctt catacaccaa cctaaacaac
     1501 caggtaagta cagaagaggt caaatctctc ttatcagctc acttggatcc aaatagtgtt
     1561 gatgcatttt ttaatctcgt taatgactat aataccattg tcggctcaac tggcttatca
     1621 ggagatttca cttcctttac tcacaccgaa tacgatgttg agaaaatcag tcatctctgg
     1681 aatcaaaaga agggcgattt cgttgggacc aactgccgta tcaatagtta ttgtcttttg
     1741 aaaaattcag tcaccattcc aaagcttgaa aagaatgacc agttgctttt cctagataat
     1801 gatgcgattg ataaaggaaa ggtctttgat tcacaagata aggaagagtt tgatattcta
     1861 ttttcgagag ttccaactga ggcaactaca gatgtcaagg ttcacgctga aaagatggaa
     1921 acattcttct cacaatttca attcaatgaa aaagctcgaa tgctgtctgt agtcttgcac
     1981 gacaatctgg atggcgagta tctgtttgta ggccacgttg gggtcttagt acctgctgat
     2041 gacggtttct tatttgtaga gaaattgact ttcgaagagc cctaccaagc gattaaattt
     2101 gctagtaagg aagattgcta caagtatttg ggcaccaagt atgcggatta tacaggcgag
     2161 ggactggcta agccttttat catggataat gataagtggg ttaaacttta atagtgttat
     2221 gatatgacag taatcttagg atttttattt ctatttttga taagttggtg ggttatttca
     2281 atcgttaata gccagaaacc acgcaaaaat gaaacattta ttggctatgt ccaaagatac
     2341 gatattgatg ggaatggc
//
LOCUS       DAVAAL010000174         2310 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVAAL010000174 DAVAAL010000000
VERSION     DAVAAL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.176"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            171..>2310
                     /gene="pcrA"
                     /locus_tag="VQM60_001954"
     CDS             171..>2310
                     /gene="pcrA"
                     /locus_tag="VQM60_001954"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET1081773.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWS"
BASE COUNT          655 a          471 c          515 g          669 t
ORIGIN      
        1 gtattgagct agtttatgaa aaaattgggt gaaaaagtcg agtgttttag aaacccacag
       61 tgtagtattc tagtttcaat ccactatatt ttgctactcc ccgtaaagtt tctattttcc
      121 ctgatttctg atataataga aatattgact tcaagagtaa ggaagagaag atgaacgcat
      181 tattaaatgg aatgaatgac cgtcaggctg aggcggtgca aacgacagaa ggtcccttgc
      241 taatcatggc aggggctggt tctggaaaga ctcgtgtttt gacccaccgt atcgcttatt
      301 tgattgatga aaagctggtc aatccttgga atatcttggc cattaccttt accaacaagg
      361 ctgcgcgtga gatgaaagag cgtgcttata gcctcaatcc agcgactcag gactgtctga
      421 ttgcgacctt ccactccatg tgtgtgcgta ttttgcgtcg cgatgcggac catattggct
      481 acaatcgtaa ttttacaatt gtggatcctg gtgaacagcg aacgctcatg aaacgtattc
      541 tcaaacagtt gaacttggat cctaaaaaat ggaatgaacg aactattttg gggaccattt
      601 ccaatgctaa gaatgatttg attgatgatg ttgcttatgc tgcccaagct ggcgatatgt
      661 atacgcaaat tgtggcccag tgttatacag cctatcaaaa agaacttcgt cagtctgaat
      721 ccgttgactt tgatgatttg attatgctga ccttgcgtct ctttgatcaa aatcctgatg
      781 ttttgaccta ctaccagcaa aaattccaat acatccacgt tgatgagtac caagatacca
      841 accacgctca gtaccaattg gtcaaactct tggcttcccg ttttaaaaat atctgtgtgg
      901 ttggggatgc ggaccagtct atctacggtt ggcgtggtgc tgatatgcag aatatcttgg
      961 actttgaaaa ggattacccc aaagccaagg ttgttttgtt ggaggaaaat taccgctcaa
     1021 ccaaaaccat tctccaagcg gccaacgagg ttattaaaaa taataaaaat cgccgtccta
     1081 aaaatctctg gactcaaaac gctgatgggg agcaaatcgt ttactatcgt gccgatgatg
     1141 agctggatga ggctgtattt gtagccagaa ccatcgatga acttagtcgc agtcaaaact
     1201 tccttcataa ggattttgca gttctctatc ggactaatgc ccagtcccgt acaattgagg
     1261 aagccctgct caagtctaac attccttata ccatggttgg cggaaccaaa ttctacagcc
     1321 gtaaggaaat tcgcgatatt attgcttatc tcaaccttat tgctaatttg agtgacaata
     1381 ttagttttga gcgtattatc aacgagccta aacgtggaat tggtctaggt acagttgaga
     1441 aaatccgtga ttttgcaaat ttgcaaaata tgtctatgct ggatgcttct gctaatatta
     1501 tgttgtctgg tatcaagggt aaggcagccc aatctatctg ggattttgcc aatatgatgc
     1561 ttgatttgcg ggagcagcta gaccacttaa gcattacaga gttggttgag tccgtcctag
     1621 aaaaaacagg ttatgtcgat attcttaacg cccaagcgac tctagaaagc aaggcacggg
     1681 ttgaaaatat cgaagagttt ctttctgtta cgaagaactt tgatgacacc acggatgtga
     1741 cagaagagga aactggtctg gacaaactga gtcgtttctt aaatgacttg gctttgattg
     1801 ccgacacaga ttcaggtagt caggagacat cagaagtgac cttgatgacc ctgcatgctg
     1861 ccaaaggtct cgaatttcca gttgtctttt tgattgggat ggaagaaaat gtctttccac
     1921 ttagtcgtgc gactgaagat ccagatgaat tagaagaaga gcgccgtcta gcctatgtag
     1981 gtatcacgcg tgcagagaaa attctctatc tgaccaatgc caactcacgc ttgctttttg
     2041 gtcgtaccaa ttataaccgt ccgactcgtt ttattaacga aatcagttca gacttgcttg
     2101 agtatcaagg tctggctcgt cctgcaaata caagctttaa ggcatcatat agcagtggta
     2161 gtatttcctt tggtcaaggt atgagtttgg ctcaggctct tcaagaccgt aaacgcggtg
     2221 ctgccccaaa atcaatccag tcaagcggtc ttccatttgg tcaatttaca gctggcgcaa
     2281 aatcagcatc tagcgaggca aattggtcca
//
LOCUS       DAVAAL010000175         2241 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVAAL010000175 DAVAAL010000000
VERSION     DAVAAL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.178"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            44..1090
                     /locus_tag="VQM60_001955"
                     /pseudo
     CDS             44..1090
                     /locus_tag="VQM60_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849941.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
     gene            1365..1667
                     /locus_tag="VQM60_001956"
     CDS             1365..1667
                     /locus_tag="VQM60_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081774.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKLVSINE"
     gene            1723..2151
                     /locus_tag="VQM60_001957"
     CDS             1723..2151
                     /locus_tag="VQM60_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081775.1"
                     /translation="MDGITKDFIKTAKLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFT
                     EVEGDTIVGLALCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAK
                     NKGCKEFASDCTLTNTDSIRFHLNIGFQEANRIIHFKKKL"
BASE COUNT          777 a          321 c          471 g          672 t
ORIGIN      
        1 aattgaccgt cattttggag acaaggagac ctttcgggaa ctggtggatc aagcgcatca
       61 tcgtggcatg aaagtcatgc tggatgcggt atttaatcat attggttcgc aatctcttca
      121 atggaaaaat gtcgtcaaaa atggtgaaca gtctgcttat aaggattggt tccatattca
      181 acaattccca gtgacaactg aaaagctagt taataagaga gacttaccct atcatgtttt
      241 tggtttcgag gactatatgc ctaagctaaa tacagccaat ccagaggtca agaattatct
      301 tttaaaggtt gcgacttatt ggattgaaga gtttaatatc gatgcttggc gtttggatgt
      361 ggctagtgag attgaccatc agttctggaa ggattttcgt aaggcagttt tagctaaaaa
      421 tcctgatctt tatatcctag gagaagtctg gcatacatct cagccttggc taaatggaga
      481 tgagttccat gccgtcatga attatccttt atctgatagt atcaaggact atttcttacg
      541 aggaattaag aagacagacc agttcatcga tgaaatcaat tgagagtcta tgtattacaa
      601 gcagcagatt tcagaggtca tgtttaatct cttggattca catgatacag agcgaatcct
      661 gtggacggcc aatgaagatg ttcaactggt taaatcagcc ttagcctttc tcttttaaca
      721 aaaaggaaca ccgtgcattt attacggaac cgagctagcc ttgactggag gaccagatcc
      781 agattgtcgt cgttgtatgc cttgggaacg tgtatcaagt gacaatgata tgctgaactt
      841 tatgaagagg ctgattaaga ttcggaaata cgcgtcagta atcatttcgc atggcaagta
      901 tagccttcaa gaaatcaaat ctgatctagt agctctggaa tggaaatacg aaggatggat
      961 cctcaaagca atattcaacc aatcaacaga agattatctt ttagagaaag aagcagtagc
     1021 actagcaagc aattgccaag aattggagaa tcagcttgtc atctctccag atggatttgt
     1081 gattttctaa aaactagttg atgaagatta tggtacattt catatcttat atagtataat
     1141 aaggctagtt actaaacttg taaaggagaa cttaaatgaa ttgtagagga catgaaacaa
     1201 gacaaagaat tgttagagat tttgaagttt agcctaaagc acatattaag ctgttagcaa
     1261 atcaacaaaa acatagtgat gcaggagcaa ctattgaaga tgaatattat gtatttatcg
     1321 ctgagagtaa aattgatggc aagaaggaag ttattcagtg ttgcatgggt gcggcaaggg
     1381 attttttaga actaattaat cacaaagggc tacctctttt taatccgctt gtaggtgatt
     1441 ctcatgtaaa taatagacaa gaatatgaca atacagggag tggaaattta caacctgaaa
     1501 agtggaatga aactgcaaag cagctttata atgttataat gtggttgatt attttatgga
     1561 atgctaagcc ggatacacct ttatttaatt ttaaagacga agtaattaag tataaaacat
     1621 atgagccttt tgaaagcagt ataaaattgg tatcaatcaa tgagtaacaa agtatcagca
     1681 tgattcgaaa catttacttg taatttgcgg aggaacatca atatggatgg aataacaaaa
     1741 gattttataa aaacggctaa actaatgaaa caattatggc cccaattgac cgataaagaa
     1801 gctattgatg aagtaaaaaa atatacgaat ggcaaaaata ctgcaatctt tactgaagtt
     1861 gaaggtgaca caattgtagg tctagcacta tgttcactca gatttgatta tgttgaaggt
     1921 tgtaaatata gtcctgttgg attcttagaa gggattattg ttgacgagga atatcgttta
     1981 aaggatattg ctaaaaatct ctgtacaaaa tgtgaggaat gggcgaaaaa taaaggatgt
     2041 aaggaatttg caagtgactg tactttaacg aatacggatt ctataagatt tcatctcaat
     2101 attggatttc aggaggcaaa tagaattatt cattttaaga aaaaattata atttagaaac
     2161 gtgaataaac ttgtttaaac tttatgtatc tttagaaagt gtgttaactt gaaagaacaa
     2221 atttttttga tgggtgggaa t
//
LOCUS       DAVAAL010000176         2200 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVAAL010000176 DAVAAL010000000
VERSION     DAVAAL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.179"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            15..1076
                     /gene="dinB"
                     /locus_tag="VQM60_001958"
     CDS             15..1076
                     /gene="dinB"
                     /locus_tag="VQM60_001958"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET1081776.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TILSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            complement(1223..2068)
                     /locus_tag="VQM60_001959"
     CDS             complement(1223..2068)
                     /locus_tag="VQM60_001959"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET1081777.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
BASE COUNT          731 a          457 c          437 g          575 t
ORIGIN      
        1 aaaaggtgct aggcatgttg atttttcctt tgttaaatga tttgtcaaga aaaatcatcc
       61 atattgacat ggatgccttt tttgctgcgg tggaaatcaa ggataatcct aaactcagag
      121 gaaaacctgt cattattgga agcgaccctc ggcaaacagg tggacgggga gtcgtttcta
      181 cctgtagcta tgaggctcga gcttttggtg tccattctgc catgagttcc aaggaagctt
      241 atgaacgttg tccccaggcc gtctttatct caggaaatta tgagaaatac aaagctgtgg
      301 gactccagat tcgagctatt tttaagcgct atacagattt gattgaaccc atgagcattg
      361 acgaagccta tttggatgtg acagaaaata aactcggtat caagtcagcg gtcaaaattg
      421 ctcgcctcat tcaaaaagat atctggcaag aactccatct aactgcttct gccggtattt
      481 cctacaacaa gttcttagct aaaatggcga gtgattatca aaaacctcat ggtttgacag
      541 tgattctacc tgaacaggct gagaattttc tcaaacaaat ggatatttcc aaatttcatg
      601 gagtaggaaa aaagacagta gaacgtcttc atcaaatggg cgtttttact ggtgctgatt
      661 tacttgaagt tcctgaggtg accctaatag accgttttgg tagactaggc tatgatctgt
      721 atcgaaaggc tcgtggcatt cacaactctc cagtcaaatc caatcgcatc cgtaaatcaa
      781 tcggcaagga gaaaacctac gggaagattc tccgtgctga ggaagatatc aaaaaagagc
      841 tgactattct atcagaaaaa gtcgctctca atctacatca acaagaaaaa gctggaaaaa
      901 ttgtcatttt gaaaatccgc tacgaggact tttcaactct taccaaacga aaaagtcttg
      961 ctcaaaaaac acaagatgct agtcagataa gccaaatagc cctgcaactc tatgaagaat
     1021 taagcgagaa agaaagaggt gtccgcctat tggggattac cctgactgga ttttaaagct
     1081 caatgaaaat caaagagcaa actaggaagc tagtcgcagg ctgctcaaaa cactgttttg
     1141 aggttgcaga taaagctgac gcggtttgaa gaaattttct aagagtataa aaccttgaag
     1201 agttgcccct tcaaggtttt tcttatacaa ataaacggac aaaactgtaa agtagcaaaa
     1261 cactaccaag cacgatacgg tatttaccaa aaagggtgaa gtcgtgtttt ttcacatagc
     1321 tggtcaagaa gcgaatagcc accatgctga ccgcaaaagc tactcccatc gcgaccaaga
     1381 gcaaaaacaa ttgcccaaag ctcaagagtt ctccggcttt cacaaatttg aaaatcttta
     1441 aggcactagc tccaaacata acgggaatcc caagatagaa ggtaaattct gtcacaactg
     1501 aacgactggt tccatttaac aaaccaccga caatcgttgc acctgaacgg ctagtccctg
     1561 gtaaaagagc aagaacttgg aagagtccga tatagaaagc ggtcgtataa ggaagcttgt
     1621 ccaactctgt tacacttggc tcgatagcac gcgctttatt gcgcttttcc aaatagatga
     1681 aggcaacccc gtagataatc aacatgagag caactgaaac catgttatgg aagtgggtat
     1741 caaaccaatc atcaaattta aagacaccaa gtaaaggcaa agtggcaatc aagaccttca
     1801 accatagtct ccaagtctta cgaacttcct gtttgtcctt agtcggttta aaaggattga
     1861 gcttgttaaa ataaatcacc ataactgcta aaatagcacc aagctgaatc acgacattaa
     1921 acatggacat aaaggcttca ttttgatttt ggtattggat aaactcctct gctaaaatca
     1981 agtgacctgt actggaaatc ggcaaccatt ccgtaattcc ttcaacaatc ccgaagaaga
     2041 tagattttaa aatttcaata agatacatat atttctcctt atattatatt taacgcagtt
     2101 agctactgcc aaagcccaag tggtatactt ggaataagcc actgtggatt agttcatttc
     2161 ctccaatcgc ttgacgattt ccgaaagggt ctaaccacag
//
LOCUS       DAVAAL010000177         2186 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVAAL010000177 DAVAAL010000000
VERSION     DAVAAL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.180"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            1..1026
                     /locus_tag="VQM60_001960"
     CDS             1..1026
                     /locus_tag="VQM60_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET1081778.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1016..2023
                     /locus_tag="VQM60_001961"
     CDS             1016..2023
                     /locus_tag="VQM60_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET1081779.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIALSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2023..>2186
                     /locus_tag="VQM60_001962"
     CDS             2023..>2186
                     /locus_tag="VQM60_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET1081780.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETV"
BASE COUNT          773 a          309 c          378 g          726 t
ORIGIN      
        1 atgaaaaaca aattttttct aatagctatt ttagctatgt gtatagtttt tagcgcttgt
       61 tcttctaatt ctgttaaaaa tgaagaaaat acttctaaag agcatgcgcc tgataaaata
      121 gttttagatc atgctttcgg tcaaactata ttagataaaa aacctgaaag agttgcaact
      181 attgcttggg gaaatcatga tgtagcatta gctttaggaa tagttcctgt tggattttca
      241 aaagcaaatt acggtgtaag tgctgataaa ggagttttac catggacaga agaaaaaatc
      301 aaagaactaa atggtaaagc taacctattt gacgatttgg atggacttaa ctttgaagca
      361 atatcaaatt ctaaaccaga tgttatctta gcaggttatt ctggtataac taaagaagat
      421 tatgacactc tatcaaaaat tgctcctgta gcagcataca aatctaaacc ttggcaaact
      481 ttatggagag atatgattaa aattgattca aaagccttag gtatggaaaa agaaggtgat
      541 gagttaatca aaaatactga agctcgtata tccaaagaat tagaaaaaca tccagaaatc
      601 aaaggaaaaa tcaaaggaaa aaaagtatta tttactatga ttaatgctgc agatacatca
      661 aaattctgga tttatactag caaagatcca agagcaaatt atttaacaga tttaggtcta
      721 gttttccctg aatcattaaa agaatttgag agtgaagata gttttgcaaa ggaaatttct
      781 gcagaagaag caaataagat aaatgatgct gatgtaatca taacttatgg tgatgataaa
      841 actcttgaag ctttacaaaa agatcctctt ttaggtaaaa taaatgcaat taaaaatggt
      901 gccgttgctg taattccaga taatacaccg ttagcagcct catgcactcc aacaccactt
      961 tcaataaact atactattga agaataccta aatcttttag gaaatgcatg caaaaatgcg
     1021 aaataaaaaa caaataaacc taggcataat ttttgtaatc tgcctaggtc ttcttattac
     1081 aatatttttg tcattaaagc ttggaacaaa agaaattaat atcagagatt ttttagcagc
     1141 ttttggaatg ggtaatacaa atgatgattt tattaaatca attatatata aaagaatacc
     1201 tagaactatt tttgcaattt tagcaggttc tagtcttgcc ataagcggtg tgttgatgca
     1261 atcagttact agaaacccaa tagctgatcc aggtatactc ggtataaaca caggagcaag
     1321 tcttagtgta gtaattgctc tttctttttt aggaatttca tcaagcataa gccatataag
     1381 ttttgcaatc attggtggct tagtaagtgc aatttttgta tacgcgattg ctgtaagcgg
     1441 aaaagcaggc cttaccccta taaaactagc cttatcagga acttgtgtta gtatggcttt
     1501 aagcagtttt gtaagttttt taattttacc gaataataac gtcttagaca aatttagatt
     1561 ttggcaaata ggtagccttg gagcagctac attatcttct atatctacac tactaccttt
     1621 tataatttta ggtcacttga tagctatatt tatttcatca gatttaaacg ctttagctat
     1681 gggtgatgaa atggctgttg gtcttggagt taatgttaat aggataagat cacttgcaat
     1741 aattgcaagt gtgcttttat gttcaagtat tactgcaatt ggtggaccta ttggcttcgt
     1801 aggtcttata gttcctcact tttgtggctt atttataagc aaagatatac gcacaatgac
     1861 catttcttca tcttttatag gtgcagagct cttgcttata tgtgatataa tcggccgtat
     1921 gttaggtaaa ccaggtgaaa ttgaagtagg gataattact gcaataatcg ggggtccagt
     1981 acttatttat gtaactatga aaaatagagg ggttaataac taatgcaaaa tttaattata
     2041 ggtattcaaa aaagaaaaaa tagaataaca ctattttcct cactattttt attaataata
     2101 atcagtctat catttttcat tttacttatc ggagatgaaa gttattcttt ttcaactttg
     2161 attaaagtct taaatagtga aactgt
//
LOCUS       DAVAAL010000178         2131 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVAAL010000178 DAVAAL010000000
VERSION     DAVAAL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.181"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            154..1500
                     /gene="gdhA"
                     /locus_tag="VQM60_001963"
     CDS             154..1500
                     /gene="gdhA"
                     /locus_tag="VQM60_001963"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET1081781.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFNPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV"
     gene            complement(1789..>2131)
                     /locus_tag="VQM60_001964"
     CDS             complement(1789..>2131)
                     /locus_tag="VQM60_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081782.1"
                     /translation="LEILEETNNEQSAIRLVNLGVEFLENNNQKLILFNLLRAKGFGK
                     KSFQEIHFTPYSHFYTGSHVPVLELEKELLERIKKIFETGIDYINLLLYLNKLIDCKR
                     KAIERELEKEF"
BASE COUNT          672 a          395 c          387 g          677 t
ORIGIN      
        1 ccaagaaaaa cgttcgtttt tttatctatt ttcaaaaaaa ccaaaaactg aattgaaaga
       61 atttttagaa aatttctgtt ttttctttac agtgaaaaaa aattctgcta taatgtctta
      121 tgtaataaaa tatgacaata aaaggagaac gatatgacat ctgctaaaga atatatccaa
      181 agcgtgtttg aaactgtgaa agctcgtaac gggcacgagg ctgaattcct ccaagctgtt
      241 gaagaatttt tcaacacttt agaacctgta tttgaaaaac accctgagta tatcgaagaa
      301 aatatcttgg cacgtattac tgagcctgag cgcgtggttt ctttccgtgt tccttgggtt
      361 gaccgtgatg gaaaaattca agtaaaccgt ggttaccgtg ttcaattcaa ctcagctgtt
      421 ggaccataca aaggtggact tcgtttccac ccaactgtaa accaagggat cttgaaattc
      481 ctcggatttg aacaaatctt taaaaatgtc ttgactggac ttcctatcgg tggaggtaaa
      541 ggtggatcag acttcaatcc taaaggtaaa acagatgctg aagtgatgcg cttctgccaa
      601 agcttcatga ctgaattgca aaaacacatc ggaccatcac ttgacgtacc tgctggtgat
      661 atcggtgttg gtggacgtga gattggttac ctttacggtc aatacaaacg tcttaaccaa
      721 tttgatgctg gtgtcttgac tggtaaacct cttggatttg gtggtagctt gattcgtcca
      781 gaagcaactg gttacggttt ggtttactat actgaagaaa tgctcaaagc taacggtaac
      841 agctttgctg gtaagaaagt cgttatttca ggttctggta acgttgctca atatgctctt
      901 caaaaagcaa ctgaactcgg tgcaactgtt atttctgttt ctgactcaaa tggttatgtc
      961 atcgatgaaa atggtatcga cttcgatctt ttggttgatg ttaaagaaaa acgtcgtgct
     1021 cgtttgactg agtatgcagc tgaaaaagca actgcaacct accacgaagg tactgtatgg
     1081 acttacgctg gaaactatga cattgctctt ccatgtgcca ctcaaaacga aatcaacggt
     1141 gaagcagcta aacgtttggt tgctcaaggc gttatctgtg tatctgaagg tgccaacatg
     1201 ccaagcgacc ttgatgccat caaagtttac aaagaaaatg gtatcttcta cggacctgca
     1261 aaagctgcca acgctggtgg tgtagccgtt tcagcccttg aaatgagcca aaacagcctt
     1321 cgcctctcat ggactcgtga agaagttgat ggacgtctca aagacatcat gaccaacatc
     1381 tttaacacag ctaaaacaac ttcagaaaca tatggtcttg ataaagacta ccttgcagga
     1441 gctaacattg ctgcctttga aaatgtagca aacgctatga ttgcccaagg cgtggtataa
     1501 aaaaaaatta cggaaactga ctacccagac gggtagccag ttttttcaat tcctaaaatt
     1561 taagattcta atacccttta tgcaaaaata tagaattcta cctttttctt tatataggct
     1621 aatgttttta atatatttat tgtctatttt tgaaaagaca ataaaactat tctactatag
     1681 attatcattt cccacaattc ataactcaaa cctaatattt tgttataaaa cagtagattt
     1741 tatttattat atctggcacc ttaaaaagta agaaatataa attaaaattt aaaactcttt
     1801 ttccaattct ctttcaatag cttttcgctt gcaatcaatt agtttattca agtacagtag
     1861 caagttaatg tagtctatac ccgtttcaaa tatcttttta attctttcta ggagttcttt
     1921 ttctaattcc aacaccggaa catgactacc agtataaaaa tgtgaatagg gagtaaaatg
     1981 tatttcttgg aatgacttct taccaaatcc tttagctctg agaagattaa atagtattag
     2041 tttttgattg ttgttttcta aaaattctac acctagattg accaatctaa ttgcagattg
     2101 ctcattattt gtttcctcca gtatttctaa t
//
LOCUS       DAVAAL010000179         2112 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVAAL010000179 DAVAAL010000000
VERSION     DAVAAL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.182"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..208
                     /locus_tag="VQM60_001965"
     CDS             <1..208
                     /locus_tag="VQM60_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET1081783.1"
                     /translation="RKSSKLKPLTVKDKAYNHALSKERSKVESILAKVKTFKMFSTTY
                     RNHRKRFGLRMNLIAGIINHELGF"
     gene            358..591
                     /locus_tag="VQM60_001966"
     CDS             358..591
                     /locus_tag="VQM60_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HET1081784.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGCAAG
                     GVKYGRLLGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            835..1068
                     /locus_tag="VQM60_001967"
     CDS             835..1068
                     /locus_tag="VQM60_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="HET1081785.1"
                     /translation="MDTKMMSQFAVMDNEMLARIEGGDVSDIYRGYANQVSPFGSYPP
                     ILKNSGPFPVSGYCPRGYHDRGYIGAGFHLCGI"
     gene            1100..1789
                     /locus_tag="VQM60_001968"
     CDS             1100..1789
                     /locus_tag="VQM60_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET1081786.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYVTLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFLSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1831..2064
                     /locus_tag="VQM60_001969"
     CDS             1831..2064
                     /locus_tag="VQM60_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081787.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
BASE COUNT          621 a          349 c          410 g          732 t
ORIGIN      
        1 acgtaaatcc agcaaactca aaccgctaac agttaaagat aaagcctata accatgcgtt
       61 atctaaggag agaagcaagg ttgagagcat ccttgccaaa gtaaaaacgt ttaaaatgtt
      121 ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgattgctgg
      181 cattatcaat catgaactag gattctagtt ttgcaggaag tctaatgtaa tttttgcaat
      241 tcaagacgtt tcgatgacta ttcaagatct ggatgaaaaa ttttaaaaaa cgatgatata
      301 ctaaacttgt caaagttaca acaagacaaa aattaaaaat aaaaaagagt atttgtcatg
      361 gatacaaaaa ttatggaaca atttcatgaa atggatataa ctatgttatc tagtattgaa
      421 ggaggcaaga ataattggca aactaatgtc ttagaaggtg gtggtgctgc ttttggttgt
      481 gcagcgggtg gagtgaaata tgggagactt ctaggaccat ggggcgctgc aataggagga
      541 attggaggag cagtggtttg tggatattta gcctataccg ctacatcata aaaatgaaag
      601 ctaaatttta tgaaggctat attctttatt attctcttcg cttttcaaac ctatcttatt
      661 tatctatcca taagtatctc cgataaaaaa caaaagacta ttgaattaac atcattgaat
      721 tgttttgtca tattatttct aatctatgat aaattaattt ttctctttat tgcctatgtt
      781 tttttgataa tttttatatt aaacttgttc cgtaactgaa aagtgatata ctatatggat
      841 acaaaaatga tgtcacaatt tgcagttatg gataatgaaa tgcttgctcg tatagagggt
      901 ggggatgtat ctgatattta tagagggtac gctaatcagg taagtccatt cggttcttac
      961 cctcctatat taaaaaattc aggtcctttt ccagtaagtg gttactgccc gcgcggttat
     1021 catgaccgtg gttatatagg agcaggtttt catttatgtg gaatataggc tgaaatacaa
     1081 gaaatgagga agaatttcta tgaaaaagta tcaacttcta ttcaaaataa gtgcagtttt
     1141 ctcttactta tttttcgtat ttggtctttc tcagatgacg cttattattc aaaattattg
     1201 gcaattttct tcccagattg gcaatttcgt ctggattcaa aatttcttga gtttgctatt
     1261 tagcggagtc atgatttgga ttctggttaa gacaggtcat ggttatctct ttcacattcc
     1321 aagaaaaaaa tggctttggt attcgatttt gacagtatta gtggtagtgc tccagatctc
     1381 ttttaacgtt cagacagcta aacatgttca gtcaactgct gaaggttggg ctgtattgat
     1441 aggttatagt gggaccaact ttgctgagct aggtatctat gtaaccctgt ttttcctgac
     1501 tccactaatg gaagagttga tttatagggg attacttcaa cacgccttct ttaagcattc
     1561 gcgatttggc cttgatttgc ttcttccttc cattttgttt gctcttcctc attttttgag
     1621 tctgcctagt ctgttagata tcttcgtctt tgcaacattt ggaatcatct ttgccggttt
     1681 gacccgctat accaagagta tttatccatc ctatgcggtg catgtgatca ataatattgt
     1741 agcgaccttc ccgtttttgc tcacttttct acatagggtc ttggggtaaa aaaacaaaag
     1801 gcttgctttt cagccataga ggaggtcatc atgtataaac acttattttt cctagattcc
     1861 aaaactttag atcggttgac accctatatt ctagtcttgg cttctgacac cattgctttt
     1921 aatgtttttg tgctaacctt tgtatctgcg gtggttttta atttcctaaa ttccatgcta
     1981 gctttaatgg ctatattcat aggggctggc tatgtggtcg gattttggtt actaatactc
     2041 aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc tgtacttgag tacggcaagg
     2101 cgacgttgac gt
//
LOCUS       DAVAAL010000180         2101 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVAAL010000180 DAVAAL010000000
VERSION     DAVAAL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.183"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..615)
                     /locus_tag="VQM60_001970"
     CDS             complement(<1..615)
                     /locus_tag="VQM60_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET1081788.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYD"
     gene            1239..1373
                     /locus_tag="VQM60_001971"
     CDS             1239..1373
                     /locus_tag="VQM60_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET1081789.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     "
     gene            1431..1796
                     /locus_tag="VQM60_001972"
     CDS             1431..1796
                     /locus_tag="VQM60_001972"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET1081790.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            1793..>2101
                     /locus_tag="VQM60_001973"
     CDS             1793..>2101
                     /locus_tag="VQM60_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET1081791.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     F"
BASE COUNT          774 a          352 c          350 g          625 t
ORIGIN      
        1 gtcgtatttc caacggcatg actgagaaca tccaccacgg ttggcatcac gcatactcat
       61 gtggtttgaa agagtacaac gtccagagta tgaaatacac atagctccat ggacaaaggc
      121 ttcaatttca acatctgtac gtttgcggat ctcagctaat tcttccattg aaacctcacg
      181 cgctaaaacg acacgagtca agcctagctc tttccagaac tcaagggttt catagttagt
      241 ggcactggct tgggtagaaa ggtggatttc aaggcctggt gcttcagtcg ctgcaatcat
      301 aatcaaggct gggtcagata cgataactgc tgcaatcccg atatcacgca gtttacggaa
      361 ccactcacca gcaccagctt catttccttc gtgcataacc atattagccg ctacatagac
      421 cttggcacca tacttggccg caaactgcac gccttcttcc atctgttcga aagtaaagtt
      481 tcccgcacgg ctacgaagac cataggcctg accaccgata aagacagcat ctgctccata
      541 ctgaacagct acctttagct tctctaaagt ccctgcaggt gataaaacct caggacgttt
      601 taatgttttt gtcatgattt ctcctatttg caatataaaa gtttgacatt tttccttctc
      661 attttatagt aaataaagga taaaatcaag cctagacgga ttgttttgac aattctaatc
      721 ttttagactt tgcataataa ctgttaaaca gtcgaaaatt accccagttt taacaagtag
      781 tcaaaaagtc taaaaaagta aaaaccagta tcccaagata ctgactcgta aaaatacata
      841 aattagtcct tagatgacta ctcccactct gttgctccta taatggaaaa ggattgatac
      901 agtctagaat atcgcttttt tatttattat tctgatataa aactgttaga attgattttt
      961 tattttatac caaaatatga atagagacgt agctaataag ttgaataaca tatttggaaa
     1021 aatgagaaat agcaatatta ctagaacttt tagaaacaat caatatttaa aaagaaaaaa
     1081 gctcaaagaa tttattaaac catgttataa tagatatagc tataatttta tttataacaa
     1141 gaggatagaa atgaccgaat tagaaagaaa aaatcgaaaa attagctaag aaatattctg
     1201 ataacttaaa catcaaagtt caagagagag ttcgtgaaat ggcaaatgat aataagagcc
     1261 attatttgat atacagagtt ttaggtattt catttgaaga aggagaaaat atcgatttgt
     1321 atcaaaataa aggtcgtttt ttatacaaat atgctggttc atttttagaa taagctgcag
     1381 tactatgctt taacgaaaaa tttggtacag aaaatactta aaaagttaac attcctaatt
     1441 ctgaaagtac aaaacctaag acttttgaaa ttgattgttt agtcggagaa aaacacgcat
     1501 acgaaataaa atggtgggat gcaactacag atggagacca tataactaaa gaacacacta
     1561 gaataaaagt tattcataac aaaggatata taccaattcg gttaatgttc tactatccaa
     1621 atagaactca agctataaaa attcagcaaa ctttagaaac attgtataac ggtattggag
     1681 ggaaatatta ttatggagat tctgcctggg aacatttaag agcagtgacc ggtattgatt
     1741 tacttagtat tctaacagat attgcaaata aaaaaacagg ggtaaaatca aaatgacagt
     1801 attaaaagga gataacttag aaatattaaa aactattgaa tcctcaagta ttgatttaat
     1861 ctatatggac cctcctttct ttacacagaa aacccaaaaa ttatctaata acaaaaatat
     1921 tatgtattca ttcgaagata cgtggacttc gattgaggat tacaaagaat ttttgtctgt
     1981 aagattagaa gaatgcaaaa gagtgctaaa aaatagtggc agtattttcg ttcattgtga
     2041 taaaattgca aatcatcata ttagattaat tttagataat atctttggag cagatatgtt
     2101 t
//
LOCUS       DAVAAL010000181         2079 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVAAL010000181 DAVAAL010000000
VERSION     DAVAAL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.184"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            98..1357
                     /locus_tag="VQM60_001974"
     CDS             98..1357
                     /locus_tag="VQM60_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET1081792.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKALGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1433..1568
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1607..>2079
                     /locus_tag="VQM60_001975"
     CDS             1607..>2079
                     /locus_tag="VQM60_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET1081793.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAV"
BASE COUNT          646 a          411 c          461 g          561 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccacac ctacggcaaa gtgaatctga agtggtttga
       61 agagagtaaa acttgtcttt tagaaaagga gcctataatg aaagtctttc agcatgtaaa
      121 tatcgtgact tgtgatcaag atttccatgt ttatcttgat ggaatcttag cagtcaagga
      181 ttctcaaatc gtctatgtcg gtcaagataa gccagcgttt ttagagcaag ctgagcagat
      241 tatagactat cagggagctt ggattatgcc tggtttggtc aattgtcaca cccattctgc
      301 aatgacaggt ctgagaggga tccgagatga cagcaatctc catgaatggc tcaatgacta
      361 tatctggcca gcagaatctg agtttactcc cgacatgact accaatgcgg tcaaagaagc
      421 cctgacagag atgctccagt caggaacaac aacctttaac gatatgtata atcccaatgg
      481 tgtggatatt cagcaaattt atcaggtggt gaaaacttcc aagatgcgtt gttatttttc
      541 tccgactctc ttttcttcag agacagaaac aactgctgag actataagca gaactcgatc
      601 catcatagac gaaatcttaa aatataaaaa tccaaatttc aaggttatgg tagcacctca
      661 ttctccgtat agctgcagta gagacttgct ggaagcgagt ttggaaatgg caaaagagct
      721 aaatattccg ctccatgtcc atgtggcgga gaccaaggag gagtcaggaa ttatcctcaa
      781 acgatacggc aaacgccccc ttgcttttct tgaagaactg ggttatttag atcatccgtc
      841 cgtatttgct cacggggtcg aattaaacga gagagaaatt gaacgcttgg catcttctca
      901 agtggctatc gcccacaatc ctatcagtaa cctcaaactg gcatcaggaa ttgctccaat
      961 tatccagctc caaaaagcgg gagtagcagt cggaattgcg actgactcgg ttgcttccaa
     1021 taacaatcta gatatgtttg aggaaggaag gactgcagct cttcttcaga agatgaaaag
     1081 tggggatgcc agccagtttc caatcgaaac agctctcaag gtactgacaa tcgaaggggc
     1141 taaggccctt ggaatggaaa atcagatagg aagtctggaa gtcggcaagc aagcagattt
     1201 tctggtcatt caaccacaag ggaaaattca tctccaacct caggaaaata tgctgtctca
     1261 cctggtttat gcagttaaat ctagtgatgt agatgatgtt tatatcgccg gagaacaggt
     1321 tgttaagcaa ggtcaagtcc tgacagtaga actttaaaag aaaaatcacg aaaaatttta
     1381 aaaaaagttt gcaaaaatct tgcattcttt ttttgactat gctatactta tatacggttt
     1441 aaaaaaactg cctaagacag taggggagct cgactcataa atatcctacc gaggacaaga
     1501 cgtatcatgt aaaaagaagc gtattgtact ttcgtgtcta ggtttgggcg cgtttttctt
     1561 tttgaaaaat tccccaagca aaataattac ggaggtgaac acactaatga gtgaagcaat
     1621 tattgctaaa aaagcggaac tagttgacgt agtagctgaa aaaatgaaag ctgctgcatc
     1681 tatcgtcgtt gtagacgctc gtggtttgac agttgagcaa gatacagttc ttcgtcgtga
     1741 gcttcgtgga agcgaagttg agtataaagt tattaaaaac tcaatcttgc gtcgtgcagc
     1801 tgaaaaagct ggtcttgaag atcttgcatc tgtatttgtt ggaccatctg cagtagcatt
     1861 ttctaatgaa gatgttatcg caccagcgaa aatcttgaac gacttttcta aaaacgctga
     1921 agcacttgaa attaaaggtg gtgcaatcga aggcgctgtc gcatctaaag aagagattct
     1981 tgcacttgca actcttccaa accgcgaagg acttctttct atgctccttt ctgtacttca
     2041 agcgccagtg cgcaacgttg ctcttgcagt caaagcggt
//
LOCUS       DAVAAL010000182         2079 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVAAL010000182 DAVAAL010000000
VERSION     DAVAAL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.185"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..1446)
                     /locus_tag="VQM60_001976"
     CDS             complement(<1..1446)
                     /locus_tag="VQM60_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET1081794.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMAAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIK"
BASE COUNT          609 a          469 c          381 g          620 t
ORIGIN      
        1 cttaataacg ttgatatgat tgacctctgg atcttccgtc ccttggaggg agcaaccacg
       61 ttccttggcg aattggatat agtctacaat ttctcgtgta atacgttcac caggagccaa
      121 gataggaatc cctggaggat agcacatgac aaattctcca cagacctgtc caacagactc
      181 atctaaggtc aaactttttc tctctgaata gaaggcttct tgtggagaca gcaccaactc
      241 gggctgaata tattctccag ctattaagtc cttcccatct cgtgaataga gtctcttaat
      301 atcagccaga gcaccaacca agcgctcgat gtcttggatg cggtcgccga tggaaatata
      361 ggccaagata ttgccgatat caccaaactc aatctgaatg tcgtattcgt ctcgcaagag
      421 gtcataaacc tcgatacctg ttaagcctat tccctgagtg taaactgaca acttggttac
      481 atcaaaatca cagaccgaca caccatctat taactctttt gagtaagcat agtaaccgcc
      541 aatggcattg atttcatgac gagcgtactc ggatagctca atgacttcct caaacgactc
      601 tttaccacga agggccaagt tgcgacgtga aatatccaaa ctagccatca ataagtaaga
      661 ggcagatgta gactgggtca ggtttataat ctgacgaacg tactcgggat tcatctgctc
      721 cccgattaaa agaagcgaac tttgtgtcaa actcccacca gacttatgca tggaaactgc
      781 tgccatatca gctcctgcag ccatagcaga aattggaagt ttaccagtga aatgcaaatg
      841 cgctccatgg gcttcatcta ctaaaaccat catgccagct tcatgagcca tttctgtcaa
      901 cccctttagg tctgaacaga tgccgtagta agtaggattg ttgattagga tagccttagc
      961 atctggatgg tcctttatgg cctgtgctac tcggtcattt tcaagaccta aagcgatacc
     1021 aatcttagga tctacactca tctcgatata gatgggaatg gcaccacata gaaccaacgc
     1081 attgatagca gatttatgga catttcgtgg cagaataatc ttatctcctg ccttgcaggt
     1141 tgccagaatc atagtctgca ccgatgaagt tgttccacca atcattagaa aggcatggct
     1201 agctccaaaa gcatctgcag ccagctcctc tgcatcacga ataatcgaaa taggatggcc
     1261 taaattatcc aagggtttca tcgaattgac atcaatgcct acacattttt ctcctaagag
     1321 ttcgacaagt tctggatttc cccgtccacg cttgtgacct ggaacatcaa agggaacaat
     1381 ccttttcttg cgtaacttca ccaaggcctc ataaattggg gcttggtttt gatctaactc
     1441 tttcaaaaca tactcctttc aatattttta ggaccttatg aaaactaagg aactaaaggt
     1501 tgactcatga aaaaagagca ggttttcacc tgctctgcaa tcttctgacg tgtttatttt
     1561 ggtttctgtt gagtatgtct gttcccgatg tttcctaact ctctagtagc aaatattacg
     1621 tactttattg atcgtttcac aagatgacgt gtgctttcac cacactaaaa atttatgaat
     1681 tagaacaagt gtcctaacat caacttataa aagtaggctt ttaaccctat caataatgtg
     1741 gcttttcaac cacgcttcgg cattcaaccc tgcctgtccg taggcatttt ttactcgggt
     1801 ttatatttgc tagaaaacat catctcattt tctaagcttt attactatat catgttttta
     1861 agaacttgta aagtattttt tgataaaaaa taaaaatata gtagattgaa actagaatag
     1921 tacacctctg cttctaaaac attgttagaa atagatttta ctgtcctgat cgatttgttc
     1981 tgttcttgtt tcattttact atatgttcgt aaatgaaagt tctataatga agttagccac
     2041 ccagccctcc taaaaattag atggttcaac tagccaata
//
LOCUS       DAVAAL010000183         2043 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVAAL010000183 DAVAAL010000000
VERSION     DAVAAL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.186"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..730
                     /locus_tag="VQM60_001977"
     CDS             <1..730
                     /locus_tag="VQM60_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET1081795.1"
                     /translation="FSISENLKTNDLIFVQFSKEGVEHFAFLRIALRETLTHLGGEVD
                     NPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIEKRIKYNGTFLNYFSDNLLAVAPKI
                     SPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSAIFNNLEESNELSPEKLANDLFDNN
                     LTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFENQKLSLSNGIELIVPNNVYQDAES
                     VEFIQNENGTYSILIKNIEDIQSK"
     gene            730..1344
                     /gene="pmp23"
                     /locus_tag="VQM60_001978"
     CDS             730..1344
                     /gene="pmp23"
                     /locus_tag="VQM60_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET1081796.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            1418..>2043
                     /locus_tag="VQM60_001979"
     CDS             1418..>2043
                     /locus_tag="VQM60_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET1081797.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKS"
BASE COUNT          676 a          419 c          390 g          558 t
ORIGIN      
        1 gtttagcatt tctgaaaatc tcaagaccaa tgacttgatt tttgttcaat tttctaaaga
       61 aggtgtagaa catttcgctt tcttgcgaat tgccctgcgg gagaccttga cccacctcgg
      121 aggagaagtt gataatccaa tcaagctaac tcagaataac ctgcctggat ttggaacagg
      181 agctgatgag gccttggtgg tcaatcttca gagtagcaag tatcacctga ttgaaaaacg
      241 aatcaagtac aacgggactt ttttgaacta tttttcagat aatcttcttg ctgtcgctcc
      301 taagatttct cctaaaaaat ctatcaagga actggaaaaa acagcccaga gaattgctga
      361 atcttttaac acagatgatt ttcaatttca atccaaggtc aaatcagcta ttttcaacaa
      421 cctagaagaa agcaatgaat tgtcacctga gaaattggct aatgaccttt ttgacaacaa
      481 tctgacggct cgtttgagct ttattgacca agtcaaagaa gccgtaccag aacctgttca
      541 atttgatgaa attgatgcca gtcgccaatt aaagaaattt gaaaaccaaa aactctcctt
      601 atcaaatgga attgagctca tcgttcccaa taacgtctat caagacgccg agtctgttga
      661 gtttatccaa aacgaaaatg gaacctactc tatcttaatc aaaaatatcg aggatatcca
      721 aagtaaataa tgtttaaacg aattcgaaga gtgcttgtac tagcagtctt cctttttgct
      781 ggctataaag cttaccgcgt tcatcaagat gtcaaacaag tcatgaccta tcaacccatg
      841 gtgcgagaaa tattgagtga aaaagacacc ccagcaaacg aagagcttgt gcttgctatg
      901 atttatactg aaacaaaagg aaaagaaggc gatgttatgc agtctagtga gtctgcaagt
      961 ggttccacca acaccatcaa tgataatgcc tctagcattc ggcaaggcat tcaaactctg
     1021 acaggcaatc tctatctggc gcagaagaag ggtgtagata tctggacagc tgttcaagcc
     1081 tataattttg gacctgccta tatcgatttt atcgcccaaa atggcaagga aaataccttg
     1141 gctctagcca aacagtactc tcgtgagact gttgccccct tgcttggtaa tacgactgga
     1201 aagacttata gttatattca ccccatttcc atttttcacg gtgctgaact ctatgtaaat
     1261 ggaggaaact attattattc tagacaggta cgacttaacc tttacatcat caaatgtttc
     1321 actctctttt caacatctgg ctagaccagg tgtttttgtt ataagttttc tttaagatag
     1381 atatattact ataaagaagt aaaggaggaa attccctatg agaaagaaac tctttctgac
     1441 tagtgctgcg gtcttgtggg cagtaacagc tatgaatagc gtccatgcag caacagatgt
     1501 tcaaaaagtt atcgatgaaa cctatgtcca acctgaatat gtcctaggtt cctccctatc
     1561 tgaagaccaa aaaaatcaaa ctcttaaaaa actgggctac aatgcctcaa cagataccaa
     1621 agaactcaag accatgacac ctgatgttta ttctaaaatc atgaatgtgg ccaatgactc
     1681 tagcttacag ttgtattcat cagccaagat tcaaaagcta ggtgacaaat cgccacttga
     1741 ggtcaagatt gaaacaccag aaaatatcac taaggtgact caggatatgt accgaaacgc
     1801 agcagtaacg ctgggtatgg aacatgccaa aatcactgta gcagccccta ttccagttac
     1861 aggagaaagt gctttagcag ggatttacta ttcgctagag gctaatggag ccaaggtgcc
     1921 acaagctaat aaagatttgg ctcaagaaga gctaaaagct ttgtcagata tcaatgctga
     1981 aaacaaggac aaatcaggct atgatgctaa taaattaaac gttgccctag ctgatatcaa
     2041 gtc
//
LOCUS       DAVAAL010000184         1987 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVAAL010000184 DAVAAL010000000
VERSION     DAVAAL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.187"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(31..738)
                     /gene="ccdA2"
                     /locus_tag="VQM60_001980"
     CDS             complement(31..738)
                     /gene="ccdA2"
                     /locus_tag="VQM60_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET1081798.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            complement(969..1385)
                     /locus_tag="VQM60_001981"
     CDS             complement(969..1385)
                     /locus_tag="VQM60_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081799.1"
                     /translation="MKAALESLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            complement(1410..1610)
                     /locus_tag="VQM60_001982"
     CDS             complement(1410..1610)
                     /locus_tag="VQM60_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081800.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            complement(1777..1987)
                     /locus_tag="VQM60_001983"
                     /pseudo
     CDS             complement(1777..1987)
                     /locus_tag="VQM60_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          604 a          422 c          359 g          602 t
ORIGIN      
        1 gcaaacatta cttttttcat ttgtcctcct ttattcaaaa attccagcta gaacatttac
       61 ttgtcctaat agtaacaaaa ttcccattaa aacaatgagg aaaccaccaa ttttctttag
      121 tagcatcata tgacgcttga ttttactaaa atatggcatg actagacctg aagctagtgc
      181 caataccaag aaaggaaggg ccatgcccag agtgtaaatg agagtataaa tcgctccttg
      241 ccaagcgcca ttgcctccag aagccgcaag tgctaaaaca gaacttaaaa ctggaccaat
      301 acaaggtgtc caaccaaagc taaaggtaat accaagtaaa aaagctgacc aataacgatt
      361 agagtctgat tttttaaagg taaaactttt ttgaacttct aatttcttca aatgaaaaat
      421 ttccatctgg tgaagaccca aaatgataat aatagctccc atgccatatc gaaaccaatt
      481 tgcatagaga atatgaccaa agtaaccagc accaaagcct agaataaaga aaatgagaga
      541 gataccagcg ataaagcaaa gtgttcgaat caagcctgac cagagaacct ttctcccaaa
      601 caaagaaaag ctttttgcac tttcttgatc atccaataaa atcccagcat agactggcag
      661 aagaggaaaa atacaaggag aaaaaaagga taaaacacct gctagaaaaa cagagattaa
      721 aaatactatc gtttccaata aagaaccaac tttcttaata attctaatcc tattttacta
      781 tattcaattt tatttgtaag ctttctgcta cgcaaaatcg tatcgggcac tattggacca
      841 atcttttctt ttgctagtca aggcggatct tatcccccaa aatagccaaa aagcaacgac
      901 aaggattact catcgctgct tttgtgaacg aaaatgtctt ttaggtctga catttcataa
      961 atcatgtttt acttgagttt gtcaaggatt gctttaagct cctctactag tttagtttct
     1021 gtctctgctg agccattttc ttctttcacg aaatcaaggg tttcttggag aaggttttgg
     1081 gctttggcaa ggactttttt atccgctttt tctgcatcta gctgtcctag aaccttgatc
     1141 aattccgtgc ttaattgctg gatttctgac tctttcttac ggcgaatcag ccagaaggca
     1201 atcacgccta ggagggcaag tagactgacc acaatcactc ctgctggaac tgagtttgtt
     1261 tcagtcatct tatctgaatc cttactatct tccgttcctt gttttgcatc cttcttgtcc
     1321 tgtgcaggct tgctatcgct agcatttgct ttcacatctt tgagagactc caaggcagcc
     1381 ttcacagact ctactgcagt acgcagacct tactctgtca aggcactatc ttccggagct
     1441 ttttgagcat ctaggaggac agccttggtt gcatcgattt ttggatcaga tactgttgcc
     1501 aaagctttca agcgttggtc taactcttga ctcaaggcac gaagttcaga cttgtcaact
     1561 tgctcttgag cttgtgtgct cgttgagcta gccgaagcgc ttgctaccac tctaggatct
     1621 tgagtcggag ctgagcttgg agctgggaca ggacttgcag gttgactagg agcagttatg
     1681 gtatattgaa actagaatag tacatataga cttctaaaac attgttagaa ttcgatttta
     1741 ctgtcctgat cgatttgtcc tgttcttatt tcattttact ataataaccg atggtgtggt
     1801 taatgttggt aagagaaact tctgaaacca tgcttcaaaa aagtcgctcg tcatcgtctc
     1861 ttcgtaagtc attggagcga ttaactcacc attcatttgt tagacctgca accaaagaaa
     1921 tcctctgata tcttcttctt aactgacctt ttaaagagcg accatattct cgataaaaat
     1981 aagtatc
//
LOCUS       DAVAAL010000185         1930 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVAAL010000185 DAVAAL010000000
VERSION     DAVAAL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.188"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            111..1016
                     /locus_tag="VQM60_001984"
     CDS             111..1016
                     /locus_tag="VQM60_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET1081801.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            1013..1828
                     /locus_tag="VQM60_001985"
     CDS             1013..1828
                     /locus_tag="VQM60_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET1081802.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
BASE COUNT          521 a          422 c          359 g          628 t
ORIGIN      
        1 gagacgactt ggacaagatg tttgaagagt ttgctaaact ccctgatttg aaacaagtta
       61 ctttccctga tgacaaagag aaaaaagcca aagcagaaaa gaaaaactag atgactgctt
      121 ttcaacaact cccatctagt gtacttcaaa ctggagccat ttttctctcc attatcattg
      181 aagcccttcc cttcgttctg ataggaagca ttgtctcagg gctgattgaa gtttatatca
      241 cacctgacaa ggtttatcat tttctccctc gaaatcgttg ggggagaatc ttttttggga
      301 cctttgtcgg tatacttttc ccttcttgtg aatgtggaat cgtccccatc atcaatcgtt
      361 ttctggaaaa aaaggttcca agttacacgg ccgttccttt tcttgtgaca gcacctgtta
      421 tcaatcccat tgttcttttt gcgacctatt ctgcctttgg caactccttc catgtcgccc
      481 tattacgagc tctgggttcc attcttgtgg ctgtaatact aggaattttt ctaggatttt
      541 tctggcaaga accgattcag aaagaaaatc gtctggcttg tcatgagcat gatttttctt
      601 acttgagttc tgcaaaaaaa gtttttcaag tctttgtgca ggccattgat gaattttttg
      661 atacggggcg ttatttggta tttggctgcc tctttgcttc tataatacag gtctacgttc
      721 cgactcggat tctgacctct atcagtgcga cccctctttt tgccatcctg ctcttgatga
      781 ttttagcctt tcttctttcg ctctgtagtg aggcggatgc ctttataggt gcttctcttc
      841 tctcgagttt cggtttggca ccagttctgg cctttctcgt cattggtcca atgctggata
      901 tcaaaaatat tctcatgatg aaaaattact tgaaagcacg atttatcagt cacttcataa
      961 caattgtaac tcttgtcgtc ttagtctatt ctctcttgat tggagtcatc ctatgattcg
     1021 atttttagtt ttagctggct attttgaact gactatttac ctccatctgt cgggcaaact
     1081 aaaccagtac atcaacatgc actattccta tctggcctat atctccatgg tgctttcttt
     1141 tatcttggct atcgttcaat tgtatatctg gatgaagcaa gtcaaaaccc acagtcatct
     1201 gaacagccga ttggccaaga tgacgagtat ttctcttctg gctattccac ttgtcatcgg
     1261 cttaactttc ccaactgtta gcttggattc tcagactgtt tcagctaaag gctatcattt
     1321 ccctctatcg gaaggaacgg atctagccat tcagacaagc gaagggacga caagccaata
     1381 tttgaaacca gataccagtt cttatttttc aaaatcagcc tatgaaaagg aaatgcgaac
     1441 ggcggcggat aaatacttat cccaagatag tattcagatc actaatgaaa actatatgga
     1501 ggtcatggag gctatctacg actatccaga tgaatttgag ggtaagacaa tccagttcac
     1561 aggctttgtc tataatgacc ccagtcatgc taatagccaa tttctgttcc gcttcggcat
     1621 tattcactgc atcgcagatt ctggtgtcta tggattgctg accaagggca atacccggca
     1681 gtatgaaaac aacacttgga tttcagccaa aggaaaactg gtcaatcact accataaaga
     1741 actcaaacaa aaccttccaa ccttggaaat cgacagcttt accaaagtcg ataaaccaga
     1801 aaatccctat gtatatagag ctttttaaga aaatcaagat aaaaacgaac aagttctctt
     1861 ctgaataaca gaaaaagagc ctgttcgttt tttggttata tgaaaattag tgacttgtag
     1921 attttcatct
//
LOCUS       DAVAAL010000186         1921 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVAAL010000186 DAVAAL010000000
VERSION     DAVAAL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.189"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            230..1231
                     /locus_tag="VQM60_001986"
     CDS             230..1231
                     /locus_tag="VQM60_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET1081803.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT          648 a          244 c          379 g          650 t
ORIGIN      
        1 atctacaatt ttatggtagt tttttctcat atgaatttat ttgtcgtgaa gttggaaaca
       61 ttcttaaaaa taaaggtgtg aaatgcaatt taaacttttt tgaaggggaa gaatatttgt
      121 aaaattaatt ttgtgaattt gtggatctta aataatttac taaattctct tgttaaaaaa
      181 aattaaatat tgtatagtaa tattagaatg ttcgtaatgg aggactgaaa tgaaaaatat
      241 aaatgggaaa aaggtaacta tttatgatat tgcacgttta tcaggttttt ctccaaaaac
      301 agtttcacga gttattaatg gtggagtaaa tgttaaagaa gaaacttatc aagcgattca
      361 aaaagttata gaggaattgt cgtatattcc aaatgcgtat gcaaaaaatt taactaaaaa
      421 agaagctata aatatattga tttctgtaaa aaaaatcgac tcttttcctt taatttggtt
      481 tcatacgctg ttagataaag tgttacggac gtgtaaagaa tttggtgtta atgctatagt
      541 tgagtacttt ggagaggagg atacaattag taattcaatt atatcaagca caggtagcct
      601 agtagatggt gttattgttt tttatgaaag tgtagatgat atcagaattc agtatttaaa
      661 gaagaaccat atgccttttc ttgttttcgg tgaatctcaa acgtctggag tagtctatgt
      721 atctaataat aattttcaag ctacttatga tatgatgaaa gctgtaactg aagaaaaatt
      781 taaaaatatg tggttgctta tgggaggaga atcccatgtt aataaggatc gtgaaagagg
      841 tgtgcgttct tttctgaatg ataaaaatta ctttatggat ttaaaagtca tttatggctt
      901 atctacaatt gattcagttt attcttatgc aatgcagcac ttgactaccc aaaattatcc
      961 ggacattatt tttgtttcag gagatgaaaa ggttcagggc ttgattcgtg cgtgctatga
     1021 gaaaggaatt cttattccag atgatatttc tatcattgga tttgataata ttcctatttc
     1081 tcagtattat actccagctt tgtctacaat tgctcccaat tatgttaaat tggctaaaga
     1141 aatgatagaa ggtgttttag caattattaa aggggaaagt gtcacatctg ttgaagtttc
     1201 tcctaaattt gttaggagac agagtttcta gtattgttta attcttgaaa atcctagtgg
     1261 aaaagatgga gtgttctaaa ataagttctg tttagcctcc attcagtttc tattgagttg
     1321 gatccaattt ctaacaagtg ggacatgttc atgttcgatc taaggaagac atggtcacat
     1381 tcgaatgata ttttcaaaaa aatcgtaaaa aagtcttgac aaagaaaaaa caaagtatta
     1441 aaataagttc aacaaatcag aaaagtaact atttttgatc ttcagggagc ctgtggtgat
     1501 tgtgaatagg tggttggaag tagtgaaagt gggctgattt tgaaaatgaa tttgaaacaa
     1561 tgaaaattcg gtgcgcacac cttacagtgc agcttgttgt taaacaaggc agagatgtaa
     1621 agggatagtc cctttataat tgaggtggca ccgcgttacc aacgccctca cacggaagta
     1681 tattctgtgt gtgggctttt ttctatccgt cgtttggttt atcttttatt agggccttaa
     1741 gtcgctttga tgaacttgag ttctatctac agctccttgc ctactactaa aagcaaacaa
     1801 aaaggctgga ctaatacgaa aaaagctagt gatactcaat gaaaatcaaa gaacaaacta
     1861 ggaaactagc cgcaggttgc tcaaaacact gttttgaggt tgtggataga actgacgaag
     1921 t
//
LOCUS       DAVAAL010000187         1886 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVAAL010000187 DAVAAL010000000
VERSION     DAVAAL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.190"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            210..>1886
                     /locus_tag="VQM60_001987"
     CDS             210..>1886
                     /locus_tag="VQM60_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET1081804.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALLIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQ"
BASE COUNT          584 a          341 c          421 g          540 t
ORIGIN      
        1 gctatacgta cgtaaataat gaaaagaatt aaataatagg cttgtgcgat aactatttga
       61 aacaagatta gctggttaga aaatccaaca agatagtgaa gaatctaata taaaatatac
      121 ataatttagt ttattttatg ttataaaagt tgtactaaaa gacttacgat tatcttgaat
      181 gccttaggga aacatgctat actactctta tgattatttt acaagctaat aaaattgaac
      241 gttcttttgc aggagaggtt cttttcgata atatcaacct gcaggttgat gaacgagatc
      301 ggattgccct tgttgggaaa aatggtgcag gtaagtctac tcttttgaag attttagttg
      361 gagaagagga gccaactagc ggagaaatca ataagaaaaa agatatttct ctgtcttacc
      421 tagcccaaga tagccgtttt gagtctgaaa ataccatcta cgatgaaatg cttcatgtct
      481 ttaataattt gcgtcggacg gagagacaac tgcgtcagat ggagctggag atgggtgaaa
      541 agtctggtga ggatttggat aaactgatgt cagattatga ccgcttatct gagaattttc
      601 gccaagcagg tggctttacc tatgaagctg atattcgagc gattttgaat ggattcaagt
      661 ttgacgagtc tatgtggcag atgaaaattg ctgagctttc tggtggtcaa aatactcgtt
      721 tggcacttgc caaaatgctc cttgaaaagc ccaatctctt ggtcttggac gagccaacta
      781 accacttgga tattgaaacc atcgcctggc tagagaatta cttggtaaac tatagcggtg
      841 ccctccttat cgtcagccac gaccgttatt tcttggacaa ggttgcgaca attacgctag
      901 atttgaccaa gcattccttg gatcgctatg tggggaatta ctctcgtttt gtcgaattga
      961 aggagcaaaa gctagctact gaggcaaaaa actatgaaaa gcaacagaag gaaatcgctg
     1021 ctctggaaga ctttgtcaat cgcaatctag ttcgtgcttc aacgactaaa cgtgctcaat
     1081 ctcgccgtaa acaactagaa aaaatggagc gtttggacaa gcctgaagct ggcaagaaag
     1141 cagccaacat gaccttccag tctgaaaaaa cgtcgggcaa tgttgttttg actgttgaaa
     1201 atactgctat tggttatgac ggggaagtct tgtcacaacc tatcaaccta gatcttcgta
     1261 agatgaatgc tgtcgctatc gttggtccaa atggtatcgg caagtcaacc tttatcaagt
     1321 ctattgtgga ccagattcct tttatcaagg gagaaaagcg ctttggcgct aatgttgagg
     1381 tgggttacta tgaccaaacc caaagcaagc tgacaccaag taatacggtg ctggatgaac
     1441 tctggaatga tttcaaactg acaccagaag ttgaaatccg caaccgtctt ggagccttcc
     1501 ttttctcagg agatgatgtt aaaaaatcag tcggcatgct atctggtggc gaaaaagctc
     1561 gtttgctttt agctaaattg tctatggaaa acaataactt tttgattctg gatgagccga
     1621 ccaaccactt ggatattgat agtaaggaag tgctagaaaa tgccttgatt gactttgatg
     1681 gtaccttgct ttttgtcagt catgatcgtt actttatcaa tcgtgtggca actcatgttt
     1741 tggaattgtc tgagaatggt tcaactctct accttggaga ttacgactac tatgttgaga
     1801 agaaagcaac agcagaaatg agtcagactg aggaagcttc aactagcaat caagcaaagg
     1861 aagcaagtcc agtcaatgac tatcag
//
LOCUS       DAVAAL010000188         1864 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVAAL010000188 DAVAAL010000000
VERSION     DAVAAL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.191"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..1037
                     /locus_tag="VQM60_001988"
     CDS             <1..1037
                     /locus_tag="VQM60_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081805.1"
                     /translation="ESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLT
                     RKTEILTLLKEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYP
                     LNIKAHTLEFPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPV
                     SKVTFAISSLGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIG
                     KIQDVLFPGLLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPL
                     QRIAHLIPFTYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWG
                     SSRKKEVFKV"
     misc_feature    1197..1328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1344..>1864
                     /gene="infC"
                     /locus_tag="VQM60_001989"
     CDS             1344..>1864
                     /gene="infC"
                     /locus_tag="VQM60_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET1081806.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPAT"
BASE COUNT          588 a          327 c          394 g          555 t
ORIGIN      
        1 tggaaagtcg cattgcagcc aacgagaggg ctatcaatga aaatgaagag aaactctccc
       61 aaatgtctga taccagctcg gaggaatacc agtttgctaa aaataattta gacgtgcaaa
      121 aaaatctttt gacgcgaaag acagaaattc tgactttatt aaaagaaggg cgctggaaag
      181 aggcctacta tctgcagtgg caagatgaag agaagaatta tgaatttgta tcaaatgacc
      241 cgactgctag ctctggctta aaaatggggg ttgaccgcga acggaagatt taccaagccc
      301 tgtatccctt gaacataaaa gcacatactt tggagtttcc gacccacggg attgatcaga
      361 ttgtctggat tttagaggtt atcatcccaa gcttgtttgt ggttgcaatt atttttatgc
      421 taacacaact atttgcagaa agatatcaaa atcatctgga cacagctcac ttatatcctg
      481 tttcaaaagt gacatttgca atatcctctc ttggagttgg agtgggatat gtaactgtgc
      541 tgtttatcgg aatctgtggc ttttcttttc tagtgggaag tctgataagt ggttttggac
      601 agttagatta tccctaccca atttatagct tagtgaatca agaagtaact attgggaaaa
      661 tacaagatgt attatttcct ggcttgctct tagctttctt agcctttatc gtcattgtgg
      721 aagttgtgta cttgattgct tactttttca agcaaaaaat gcctgtcctc tttctttcac
      781 tcattgggat tgttggctta ttgtttggca tccaaacaat tcagcctctt caaaggattg
      841 cacatctgat tccctttact tacttgcgtt cagtggagat tttatctgga agattatcta
      901 agcagattga taatgtcgat ctaaattgga gcatggggat ggtcttactt ccttgcctga
      961 ttatcctttt gttagtgggg attctattta tcgaaagatg gggaagttca cgtaaaaagg
     1021 aagtttttaa agtttagttt tcctataggg aagctaaaga aaaactaaca tagggagaga
     1081 atcaacttgg ttctctcttt ttgattcgaa aaccaaacca aaatataaac acaaactttt
     1141 caaaaaataa ctttttatct tgacaagagc tagaaaactt ggtatcatat aaaagttgag
     1201 aaaagcagaa gtgagagctt ctcgccttgt gacattaagt tgcctggccc tacggatgaa
     1261 aagtttcgaa gaaacgctat cataacgtgc gggcttgtat atttacgagt ccgctattgt
     1321 ttttctctaa taaaacaaaa gaggtgaaaa ccatagcaaa gcaagactta ttcatcaatg
     1381 atgagattcg tgtacgtgaa gttcgcttga ttggtcttga gggagaacag ctaggcatca
     1441 agccactcag tgaagcgcaa gctttggctg ataacgctaa tgttgaccta gtattgattc
     1501 aaccccaagc caaaccgcct gttgcaaaaa ttatggacta cggtaagttc aaatttgagt
     1561 accagaagaa gcaaaaagaa caacgtaaaa aacaaagcgt tgttactgtg aaagaagttc
     1621 gtctaagtcc aactattgac aagggtgact ttgatacaaa acttcgtaat gcacgtaagt
     1681 tccttgaaaa aggaaataaa gttaaggtat ctattcgctt taagggtcgt atgattaccc
     1741 ataaagagat tggtgcaaaa gttttagccg agtttgctga agcaactcaa gatattgcca
     1801 tcatcgaaca acgtgctaaa atggatggac gtcaaatgtt catgcaattg gcgccagcga
     1861 ctga
//
LOCUS       DAVAAL010000189         1843 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVAAL010000189 DAVAAL010000000
VERSION     DAVAAL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.192"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            39..1670
                     /locus_tag="VQM60_001990"
     CDS             39..1670
                     /locus_tag="VQM60_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET1081807.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGIGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIGLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          527 a          391 c          368 g          557 t
ORIGIN      
        1 aggagatatt ccgcgattat ttttcggagg aaaaagaaat gtccattaat tggcaggaaa
       61 ttttatttca ctttttaggt ggtctggggc tattcttata tagcatcaag accatgggag
      121 acggtttaca acaagctgct ggagatcgcc ttcgttttta cattgacaaa tatactagta
      181 atcctttctt tggagttctg gttggtattg ggatgactgc tctaattcag tctagttctg
      241 gtgtaacagt tattacagtc ggcctggtca gtgccggtct cttaacctta cgtcaggcta
      301 tcgggattgt catgggtgct aatattggga caactgtcac atcctttctc atcggtttta
      361 aattaggtaa ctatgcccta cctatgctct ttatcggtgc cgtctgtctc ttttttacaa
      421 aaaatcggac agtcaataat atcggacgca tcctctttgg tatcggtggt atcttttttg
      481 ccctcaatct catgagcggc gcaatggctc cactcaagga tttacaggtc tttaaggact
      541 atatgattga gctaagtaag aatcctgttt tgggtgtctt tgtcggtact ggcttgacct
      601 tgctaattca agcttcttcg gctaccattg ggattttaca aaacctctac gccggcaatc
      661 taattgacct acagggagct ttgccagttc tatttggtga caatatcggg acaaccatta
      721 cagccatcat tgcctcttta ggggctaata ttgcagctaa acgggtagca ggagctcatg
      781 ttgccttcaa cgttatcgga acagtcgtct gcgttatttt tctagttcct tttactgtcc
      841 tgattcattg gtttgaagct acgctaaatc tagcaccgga aatgaccatc gcctttgctc
      901 acggaacctt taatattacc aacaccattg tccaatttcc atttatcgga gctctggctt
      961 actttgtaac caagattatt cctggagagg acgaggttgt caaatacgaa cccttatatc
     1021 ttgatgaaca tttcatcaaa caggccccat ctatcgctct aggaaatgct aagaaagagc
     1081 tcttgcactt aggaaactac gctgctaaag cctttgacct ttcctataag tacatcattg
     1141 gcttggatga aaaagttgct gaaaaagggc ataaaaccga agaagcaatt aacaccatcg
     1201 atgagcaatt aacacgttat ctcattgccc tttcaagcga agctctcagc caaaaagaaa
     1261 gtgaagtgct taccaatatc cttgattcct cccgtgattt ggaacggatt ggagaccaca
     1321 cggaggctct actcaatctg actgactatc ttcaacggaa aaatgttgaa ttttctgatg
     1381 ccgccttgaa agaattagag gaagtttacc gccaaactag tgactttatc aaagatgctc
     1441 tggatagtgt ggaaaacaat gatattgaaa aagcacgcag tcttgtagaa cgtcatgaag
     1501 caatcaataa gatagaacgt gttctcagaa aaacccacat caaacgcctc aacaaaggcg
     1561 aatgttcaac acaagctggg gtcaacttta tcgacatcat ctcacactac actcgtgtat
     1621 cagaccacgc tatgaacctt gctgaaaagg tttttgcaga acaaatctaa gaaccaagaa
     1681 gctatccatc ataattggat ggcttcttac tttttcctaa gcaagactag gctgaatgaa
     1741 actgaaagag tattctgcag atatatagta tattgaaact agaatagtac acctctattt
     1801 ctaaaacatt cttagaaatc gatttgactg tcctgatcga ttt
//
LOCUS       DAVAAL010000190         1830 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVAAL010000190 DAVAAL010000000
VERSION     DAVAAL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.193"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            186..719
                     /locus_tag="VQM60_001991"
     CDS             186..719
                     /locus_tag="VQM60_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HET1081808.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKEKVKPNKKDMTREI"
     gene            846..1568
                     /locus_tag="VQM60_001992"
     CDS             846..1568
                     /locus_tag="VQM60_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HET1081809.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIDIEKDIEIDKEREIDKKSTP
                     IFYGEFKNVRLSKEEYKNLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEE
                     DKDKLRQKGLNKKMNYDVGESL"
BASE COUNT          896 a          242 c          307 g          385 t
ORIGIN      
        1 aggtggaaag gtagtgtaag ctatttttaa aagtataaaa ggtacaaaga cctaagaaat
       61 atataaaaac atcaaaataa agtattccac caccaagaac aataaaataa aaacaagcaa
      121 agggaagtat agaaaaatta aaacctatac ttcccttttt taattcaaac aaaggagaga
      181 aaagcatgat aaacgaagaa ataagcaagg aagcaggtca agcagcacaa accataatat
      241 catacacaat aaaggcaaca aaagaatcaa tcaatttaga aaaagaaata agaaaaaaga
      301 tgaatgaaac tttagaaaaa gcaaatggaa acttaaaaag tcttatgggc gatgaaatga
      361 aaataaaaga cctctacaag aaaggacaac tagaaaatat aagcatagat caaatcgacc
      421 tcaaagactt aaaaaaagaa ctaaacaaac ttggagtaag tttctcagta atgaaaaaca
      481 aagaaagcaa aaactatgaa atattcttcc aagccaaaga cataaaagta atggaatatg
      541 cctttaagca agtcatagcc aaggaaaata aaaaagaaaa agaaagtatc ctaaaacaaa
      601 taaagaaata caaagaccta tccaaaaaca aagataagac aaaagaaaaa ggaaaaagga
      661 aagtaaaaga aaaagaaaaa gtaaaaccaa acaaaaaaga tatgaccaga gaaatctaaa
      721 gggagtaacg aaaagttaca cccttaaata accataataa caagacttcc gaaaaacaga
      781 agtccagaat tccgaaaatc ggaaatcaag aatttcgatt atcgaaaatc aagaaaggag
      841 cagatatggc aacaaacaaa agatactatt ggataaaatt aaaagaagaa tttttcacag
      901 acaaaaggat aaaaaggtta aggagaatat ctggaggaga tacctatacg ataatctacc
      961 taaaacttct actactaagc ctaaaagatg agggcaaact ctattatgac ggagtagaaa
     1021 gtgattttat aaaggagtta gcactaacca tagatgaaac agacgatgat gtaatggtaa
     1081 cagtaaatta tttaatcaat caaggcttat tagaagttgt aacagaaaat gatgaatact
     1141 acctaactga aataccaaac ttaatcggat cagaaacagc ctgggcagag aaaaaaagaa
     1201 gatacagaca aaataaacag aggacattgt ccttaatgag tccaacccat gtccgacaag
     1261 agatagatat agagaaagat atagagatag ataaagagag agagatagat aaaaagtcca
     1321 cccccatttt ttatggagaa ttcaaaaatg taaggttaag caaagaagaa tataaaaatc
     1381 taaaagaaaa attaaactca cacacagata taatgataaa taaactatcc agatacatgg
     1441 aaagcagtgg taagacctat caaaaccact atgtgacaat cttaaaatgg tatgaagaag
     1501 ataaagacaa actaagacag aaaggtttaa ataaaaaaat gaattatgat gtaggagaat
     1561 ctttatagat aaaaagaggt ggaaaggcac tattaaagac tttaagtcta aaaaggtatc
     1621 aaagtatgcc agagataaaa aaatataaat atgacacttt aaaggcgatt agaaaattaa
     1681 agaaatgcta gaagactttt agatgacagt taccaagagg atctaatttt cactgctact
     1741 tatgcagtgg gaattggacc tttttatata agcaaattct atttcaaaat tggttaccaa
     1801 gaaagacttc ttttcactgt taagtataag
//
LOCUS       DAVAAL010000191         1814 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVAAL010000191 DAVAAL010000000
VERSION     DAVAAL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.194"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..188)
                     /locus_tag="VQM60_001993"
     CDS             complement(<1..188)
                     /locus_tag="VQM60_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET1081810.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIA"
     gene            complement(344..646)
                     /locus_tag="VQM60_001994"
                     /pseudo
     CDS             complement(344..646)
                     /locus_tag="VQM60_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(751..998)
                     /locus_tag="VQM60_001995"
                     /pseudo
     CDS             complement(751..998)
                     /locus_tag="VQM60_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
     gene            complement(1280..1754)
                     /locus_tag="VQM60_001996"
                     /pseudo
     CDS             complement(1280..1754)
                     /locus_tag="VQM60_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
BASE COUNT          547 a          357 c          258 g          652 t
ORIGIN      
        1 aaggcaatca gtatcaaact aacaatccaa aagattcctt ggtttcgaac caactgcaag
       61 gcgctcttgc cttcttcaat taaaatagca ctggtggtcg aatagaccac aatcaagccc
      121 aaaatagata aaagcaagta gggaatcaag atggaataat ttaataagtg cctcttacta
      181 atcttcatag tatcaccact ctactaggat acagacaaat ctgttccaaa ttccgtttta
      241 ttttctagtt ttgattgcta ttataccatt ttttcagaaa gaaaaaaact cttaccttac
      301 aatccttcga attttactgt ttttacagaa ttaaggcaga atttgaaatc gtaaaaccat
      361 tttgaataag agttgcttca acccattgac tccgacggat taagttgctt tcgtgaatgc
      421 caaaatcagc cgcaatttgt tcataagtgc tctattctcg catgtattga atagttacca
      481 taaggagatc gtctaggctt aatttgcttt ttcgtccacc ttttgcgcgt ttacgttgat
      541 aagctgtttt taacacagct aacatctctt caaaagtcgt gcgctgaaca cctacaagga
      601 tcttgaaacg gctatcgctt aattgtttac ttgcttcatc atccatagtt ttattgtatt
      661 gtatttttgt ttcgcaggaa gtctattatt cctttcgttc caaggttttt acgcttttag
      721 caacaagctt acacactgag cgtttcggac ttaccatatc atttcgttgg agattttatc
      781 atcaattttt agattaaaaa aatttcccaa tcttcacaaa cttctgtagc aggaattgta
      841 tgaatctttc taagtatctt tcctgcttct acaccatatg tgtattgttg gttttctgaa
      901 taagctaaaa tgctatctat tgcatctctt ccgtctatcc attcgtgtaa agaatgtact
      961 tcgtcatcgc agagttcaat gctaattggt ttacacatcg gaactccaag agaagctact
     1021 ttctccatca tatcaaattc aaattttttt ataatctaac ttcgacttat cagaaacacg
     1081 caagaaatat ttttactgat tttgatctgt cacacaatat tttttatcat ctgaccagcc
     1141 tttatttatg gctatcttac tactaaaatc catcaagcac actccgattc actttacaac
     1201 actttttgca gttttcacta gtaacctatc cagttcgtct acacttttat ttcctagatc
     1261 catttttaca ttatgaaagt tagtcaattt tctctatgat gtttccaagt ttatccaata
     1321 caagacaaga atgatcattt agtggaatta tgggaacatt aacgagttct tcagctctat
     1381 ccttatttgc tttatcattc caggaatcat aatggacact aattaaaaac tgactaaaga
     1441 gtcctaatcc attttttatc tttatctgat gatctgaatt atcattaggt gagacataga
     1501 ctttttctcc taatagtagg gctcctgcag aaaatcctat aatttttgcc tctttattaa
     1561 gcatatgatc gatataactt ttgaactcct gattaacata agttagcaat atatttttcc
     1621 gtatttccac caccgatgat aattatgtca gcatctagat agctatcaag atcaatctgc
     1681 tcagtgtcca ggagcaagta atcaatgttt agattaggaa attgactttg aaaaacttcc
     1741 gtgtactttt tcatgtacgg ttcccaattt tctcgaaaaa ctgtaaaaat gataattctt
     1801 ttaatcttgg ttga
//
LOCUS       DAVAAL010000192         1768 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVAAL010000192 DAVAAL010000000
VERSION     DAVAAL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.195"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..552)
                     /locus_tag="VQM60_001997"
     CDS             complement(<1..552)
                     /locus_tag="VQM60_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1081811.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQR"
     gene            1018..1356
                     /locus_tag="VQM60_001998"
     CDS             1018..1356
                     /locus_tag="VQM60_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET1081812.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKIKGTRITK"
     gene            1453..>1768
                     /locus_tag="VQM60_001999"
     CDS             1453..>1768
                     /locus_tag="VQM60_001999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081813.1"
                     /translation="MSPGTFIALTGAAVLTKKLINYIKVGGLGGWLIGAAAGVLALIF
                     LIKICVDTTIKVLLNRIFKASKVMKRRIFSLFVLDLMVYIFLGYVLVIQKDVYLFSIL
                     IIF"
BASE COUNT          570 a          239 c          366 g          593 t
ORIGIN      
        1 gcgttgcaat atttttccag tgatgagatg gaggtattca atgggaaaac tgaaaagaat
       61 ggtattgagg atttttagat cactctcata ccatgtatct tgtttttcaa tttttctcca
      121 aagttttttg acaacctgtt ctgcgtgatc tgatagttcc cctgccccgt gttgacggat
      181 ataaatgaca acttcaagca tatctctgat ttcttctata gggagatcgt ggtgggtctt
      241 gaggtagtct tggcattttt gaaataaatt gacaagatcg ctggtcccga taatagaacg
      301 catattgaga taggtttgca taatctctgt gcgttgactt ggttgataaa gttgacagat
      361 atattcaaac tcttcaaaac tcatattgat ttgacggaga agaaattcca tattttcata
      421 ttttggggtt gtcttgccac tttcgatttt tgataagctg gttcttgaga gaacaccccc
      481 acagacctct tcttgggtca gcccctttga ctcacgtatt tctttataga cctttccgaa
      541 atcataacgc atgattttct cctttctgtg aaaaaagtta acaaataata aaatcttgtt
      601 aaaaatattg tatcatagca atgtaaaaag taaaaaagaa agtcgaaata aatttgtgaa
      661 aaaagttaac gtaattttaa aatgagctta ataagaggta tgatgtaggt agaaaataaa
      721 gataggagaa aaagtatgaa taaaaaaata ttttgtattc tagtatgtat tatatcattg
      781 attagtctgg ccataatctt tccatggggc tggtcgatat aatgtaaggg agcttgagtt
      841 gttaaaggag gcttagtcct tggacaaaaa tctgctgtac tacaatgctt ggaaagatat
      901 caagaacaaa gaatgatacg attgaaacta aactgattca agaactactg ggcagtgatt
      961 gaggtttggg tgacatcaaa gaaaagttag attaattcta aagaattgag tatttctatg
     1021 ttaaaaaaat atccttgtac gatgcaacat gatcagtcag attgtgctgc ggcagttgtt
     1081 tcaacagttc ttttatctta caaaaaggaa ctatcaatca tgaagattcg ggaaattatt
     1141 ggtacagaca tgtatggaac gactgtcagt ggtattgttt caggtctgaa taagttgaat
     1201 tttacagtaa aagctgttcg agtagcactg gaagatttga ctccaaaatt aacatttcct
     1261 gcgattcttc aagttaagaa tgatttaggt caaaatcatt ttgtggtatt acatagtata
     1321 aaggagaaaa taaagggaac tcgtattacc aaataattat gttgctcttg agcaagagga
     1381 gatgatgtat cttgatgggg gggggcttat ttaagcaaga gtgcttgtca aggaatttgc
     1441 gcagctttag ctatgagtcc aggaactttt atagcattga ctggagctgc agttttaaca
     1501 aaaaaactaa taaactatat taaggttgga ggccttggag gttggcttat tggtgcagca
     1561 gcaggtgtat tggctcttat ctttttgata aaaatttgtg tagatactac gataaaggtt
     1621 ctattaaaca gaatctttaa ggcaagtaag gtaatgaaaa ggaggatatt ttctcttttt
     1681 gtactagatt tgatggtcta tatattttta ggttatgttt tagtgataca aaaagacgta
     1741 tatctgtttt caattctgat aatttttt
//
LOCUS       DAVAAL010000193         1735 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVAAL010000193 DAVAAL010000000
VERSION     DAVAAL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.196"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(267..1463)
                     /gene="tuf"
                     /locus_tag="VQM60_002000"
     CDS             complement(267..1463)
                     /gene="tuf"
                     /locus_tag="VQM60_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET1081814.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          504 a          335 c          348 g          548 t
ORIGIN      
        1 tacaacctaa aaacagtgtt ttgagcagtc tgcagctaac tttctataac aaaatgaaat
       61 aagaacaaga caaatcaatc aagataatca aatcgatttc taacaatgtt ttagaagtag
      121 atgtgtacta ttctagtttc aatatattat agtttgctct ttgattttca ttgagtatta
      181 gttcaatatc taaaaaaaga accttgccaa gcaagattct tttagtgtct gacttaaata
      241 attgttcttc tgggaactaa atcgaattaa gcttcgattt ctgtaaccat acctgaacca
      301 acagtacgtc caccctcacg gatagagaat gtagtacctt gttctacggc gattgggtga
      361 atcaactcaa cgtcgattgt cacgttatca ccaggcatta ccatttcagt acctgctgga
      421 agttcgattg aacctgtaac gtcagtagta cggaagtaga attgtggacg gtagttgttg
      481 aagaatggag tgtgacgtcc accttcttct ttagtaagga tgtagacttc acctttgaat
      541 ttagtgtgtg ggttgattga acctggttta gcgataactt gtccacgttc gatttcatca
      601 cgttgaacac cacgaagaag gacacctacg ttatctccag caagaccttc gtcaagttgt
      661 ttacggaaca tttcaacacc agtaacaact gctttttgag tttcttcttt gataccaaca
      721 atttcgattt cgtcgttgac tttaacgata ccacggtcga tacgtcctga agcaactgta
      781 ccacgtccag tgattgagaa tacgtcctcg actggaagaa gcaatggttt gtcagtgtca
      841 cgttctggtt ctgggatata ctcatcaact gtgttcatca attccataac gatgtcttcg
      901 tatttagagt caccttcaag agctttaagt gctgaacctt ggataactgg aagatcgtca
      961 cctgggaagt cgtattctga caataggtca cggatttcca tttcaaccaa ttcaagcaat
     1021 tcttcgtcgt caaccaagtc aactttgttc atgaagacga taaggtgttt aacaccaacc
     1081 tgacgtgaaa gaaggatgtg ctcacgagtt tgtggcattg gtccgtcagt tgaagctact
     1141 acaaggatag ctccgtccat ttgagcagca ccagtgatca tgtttttaac gtagtccgcg
     1201 tgtcctggag cgtcgatgtg agcgtagtga cgtttttcag tttcgtactc aacgtgcgca
     1261 gtgttgatag tgataccgcg ttcgcgttct tctggagcag catcgataga cgcatagtct
     1321 ttaggttggt taactgatga aggcaagcga cgtgccaaaa cagttgtgat agctgcagtt
     1381 agggtagttt taccgtggtc aacgtgtccg atagtaccaa tgttaacgtg tggtttacta
     1441 cgatcgtatt tttcttttgc catttgagta aaagcctcca ataaaatata ttttatagat
     1501 agacagtagg caatacagtc taactttcct tactatttta tcaaatttca gctaaattgc
     1561 aagtgtttta caacgttttt gtgaattatt cttggtaaaa atttaactaa ttaatagaaa
     1621 gtactaaata gacttatcct tcaaggagtt ttcagcatta tccatccact cacttgctga
     1681 aaatgtaaac agtctctggt ttatatagta aaatgaaaca agaatagggc aaatc
//
LOCUS       DAVAAL010000194         1719 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVAAL010000194 DAVAAL010000000
VERSION     DAVAAL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.197"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(246..1679)
                     /gene="lysS"
                     /locus_tag="VQM60_002001"
     CDS             complement(246..1679)
                     /gene="lysS"
                     /locus_tag="VQM60_002001"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET1081815.1"
                     /translation="MAALREQGIDPFGKRFERTANSQELKDKYANLDKEQLHDKNETA
                     TIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEENYEIFKKADLGDFLGVEGE
                     VMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETIYRKRYLDLISNRESFERFV
                     TRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFITHHNAQNIDMVLRIATELH
                     LKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADFQDIMDLTEGIIQHA
                     AKSVKGDGPVNYQGTEIKINELFKRVHMVDAIREITGVDFWQDMTLEEAKAIAAEKKV
                     PVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVSPLAKKNPEDQRFTDRFELF
                     IMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATGIDYDYIEALEYGMPPTGGL
                     GIGIDRLCMLLTDTTTIRDVLLFPTMK"
BASE COUNT          470 a          348 c          368 g          533 t
ORIGIN      
        1 aatctgaaca aatcaattgg ggaatccaag tcaatttcta gtactttttg aacagtttca
       61 gcgtactatc gtaaaatgaa ataaaacatg cacaaattga ttagggaatt taaaccaatt
      121 tctaacaatg tcttagaaat caaagtgtac tatttcaact tcaatatact acacttcatt
      181 ttctccttaa attcagtctc tttttgaatc aaaaaatcct ctggtaagag ccagaggata
      241 agaatttatt tcattgttgg gaagagcaat acatcacgga tagttgttgt atcagtgagg
      301 agcatgcaga gacggtcgat accgattccc aaaccacctg ttggtggcat accgtattca
      361 agagcttcaa tgtagtcata gtcgattcct gtcgcttcat catcaccaag ttctttggct
      421 ttagcttggg cttcaaaacg gctaagttgg tcgattgggt cgttgagttc agtaaaggca
      481 ttaccgtact ccttagtcat gataaagagc tcgaaacggt cagtaaagcg ttggtcttca
      541 ggatttttct tagcgagtgg agatacagct actggatgtc catagacaaa ggttggttgg
      601 attaaagttt cttcaacaaa ctcttcaaag aaggcattga tgatgtgacc aacctcagtg
      661 tagtgtttct caactggaac tttcttctca gcagcgatag ctttagcttc ttctaaagtc
      721 atgtcttgcc agaaatcgac accagtaatt tctctgatag catccaccat atgaacacgc
      781 ttaaatagtt cgttaatctt gatttcagta ccttggtagt tgactgggcc atcgccctta
      841 actgatttag cagcgtgttg gataatgcct tccgtcaagt ccatgatgtc ttggaagtct
      901 gcataagctt ggtaaacttc gatagaagta aactcagggt tatgagtagc gtccattcct
      961 tcgttacgga agatacggcc aatttcatag acacgttcca taccacccac gataaggcgt
     1021 tttaagtgaa gctcagtcgc gatacgaagc accatgtcaa tgttttgggc attgtggtgg
     1081 gtgataaatg gacgggcagc agcaccaccg gcttcattat gaagaacagg tgtttccact
     1141 tcaaggaatc ctttttggtc aaggtaacga cggatttcag agatgatttt tgaacgagtg
     1201 acaaagcgtt caaagctttc acggttagaa atcaagtcaa ggtaacgttt acggtaaatt
     1261 gtttcaacgt ctgtcaaacc atggaatttc tcaggaagag gacgaagagc cttagacaag
     1321 tgtgtgatgt gggttgcctt gatagagagt tctcccatat ccgtacgcat cacttcacct
     1381 tcgacaccaa ggaagtcacc aaggtctgct tttttgaaga tttcgtagtt ttcttcaccg
     1441 acagcatcct tacgaacgta gatctgaatc tggccttcgc ggtcttgaag gtgggcaaaa
     1501 ccaacttttc ctttaccacg tttggttatc aagcgtcctg cgatagtagc tgtttcgttt
     1561 ttatcgtgta attgttcttt atcgaggttg gcatatttat cttttaattc ttgtgaattt
     1621 gcagtacgtt caaaacgttt tccgaaagga tcgattcctt gttcgcggag cgcagccatt
     1681 ttttcacggc gaacgatctg ctggtcattt agttcttcc
//
LOCUS       DAVAAL010000195         1713 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVAAL010000195 DAVAAL010000000
VERSION     DAVAAL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.198"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            204..845
                     /locus_tag="VQM60_002002"
     CDS             204..845
                     /locus_tag="VQM60_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HET1081816.1"
                     /translation="MLRLYLENEIIELSNNLETIWVSVLDKYEKLFEYLSNEERVELI
                     KEDLIINESVLNIDKKGYELICLQHPVSYDLRRIISVIKISTDIERIGDRIVEILKNL
                     QIIQNNEMLKKIISEIKILHEDIGLHMNRAISCYREEQSGCLDMVVIQKQNEIEELSI
                     NIEKKIMNYIFEDDGNVSEVIGALDIIHHLDKIAHTTQSIYKWIMYRKYGNIN"
     gene            858..>1713
                     /locus_tag="VQM60_002003"
     CDS             858..>1713
                     /locus_tag="VQM60_002003"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="catalyzes the formation of alpha-D-glucose
                     1-phosphate and UDP-galactose from UDP-glucose and
                     alpha-D-galactose 1-phosphate in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET1081817.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEF"
BASE COUNT          667 a          185 c          311 g          550 t
ORIGIN      
        1 taatgagcga tggcttttat gaattctatc aaaataatca aaataaaact tttgaagagt
       61 taattaagat gagatttaat tcttctgcta ttgatccaat ctatggaaag aaggatgatg
      121 caagtattct agtaattgat gtttgatata ttgtttaaaa tattaattgt tactattgaa
      181 ctttgttaag aagaggtacc taaatgttaa ggttatattt agaaaatgaa attatagaat
      241 tatcaaataa tttagagacc atttgggtat ctgtgcttga taaatatgag aagttatttg
      301 aatatttatc aaatgaagaa cgagtagaat tgattaaaga agatttaatc ataaatgagt
      361 cagttctaaa tatagataaa aaaggatatg agttaatttg tttacaacat cctgtttcat
      421 atgacttaag aagaattatt agtgtaataa aaatatcaac tgacattgaa cgtattgggg
      481 atagaattgt tgaaatttta aaaaatctac agattattca aaataatgaa atgttgaaga
      541 aaattatttc agaaataaaa atacttcatg aagatatcgg actgcatatg aatagggcta
      601 tatcttgtta tagagaggaa caatctggat gtttagatat ggttgttata caaaaacaga
      661 atgagataga agagcttagt ataaatattg aaaagaaaat aatgaattac atatttgaag
      721 atgatgggaa cgtttctgaa gtaattggtg ctttggatat tattcatcac cttgataaaa
      781 ttgctcatac tacgcaatca atttataaat ggataatgta tagaaaatat gggaacataa
      841 attagaaaga aatatatatg aaaacaatta ttgataattt tgttgataga gtaattgaat
      901 acggaatgta taatgaaatt gacaaaatat atgtgaaaaa tagagtacta gcgttaatag
      961 gtgaggaggg aactgatcgc atttccgatg aaaatgacct gaaacaaata aaagattact
     1021 tagtggaaat cgccttaaaa aacggtaaaa ttaaagattt aatcgaggaa aaggaatgtt
     1081 taggggcaga gttaatgaat ttcatagttc ctttacctag tcgattaaat gatatttttt
     1141 ggagttcata tgatatatca cctcaagaag cagttgaaga gttttataag ttaagtaaag
     1201 atagtgatta tataaaaact tctgctattg ctaagaatat tgagtttcga gcatcaacta
     1261 aatacggaga gctagagatt acgataaatt tgtcaaaacc cgaaaaagat ccaaaaacaa
     1321 ttgctgctga aaaattagta aaagcaacta attatcccaa atgtttacta tgtatggaaa
     1381 atgaaggata tcaggggcga ataaattatc cagcacgttc gaatcataga attattcggt
     1441 tgaaacttgg tgacgaagta tggggattcc agtattctcc ctactcatac tttaatgagc
     1501 atgcgatatt tttgaatagt caacatgtac caatggctat tacctctaaa acatttgaac
     1561 aattattgga gattgttgat attttgccag gatattttgc tggttcaaat tctgatcttc
     1621 ctatctcagg aggctccatt cttagtcata accactacca gggagggaaa catattttcc
     1681 caatggaaaa agcaaaattt gagagtgaat ttc
//
LOCUS       DAVAAL010000196         1690 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVAAL010000196 DAVAAL010000000
VERSION     DAVAAL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.199"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..71
                     /locus_tag="VQM60_002004"
     CDS             <1..71
                     /locus_tag="VQM60_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HET1081818.1"
                     /translation="IPAMSAPVFDNIEAAIKEHGLS"
     gene            119..1594
                     /locus_tag="VQM60_002005"
     CDS             119..1594
                     /locus_tag="VQM60_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET1081819.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          475 a          340 c          343 g          532 t
ORIGIN      
        1 gtatcccagc aatgagcgct ccagtatttg acaatattga agcagctatt aaagaacatg
       61 gattaagcta attctaattt gttaacattc atataacata aaaaagggag gaactgttat
      121 ggatgcaatc tttgacctaa tcggaaaggt tttcaatccc atcttagaaa tgggtggacc
      181 tgtcatcatg ttgatcattt tgacagtatt ggctttactt tttggagtga aattctccaa
      241 agcgcttgaa ggtggtatca aacttgccat cgctcttaca ggtatcggtg ctatcatcgg
      301 tatgctaaat ggtgctttct cagcatcact tgcaaaattc gttgaaaaca ctggtatcca
      361 attgagtatt acagacgttg gttgggcacc actcgcaaca atcacttggg gttctgcttg
      421 gacactatac ttcttgctca tcatgttgat tgtcaacgta gtgatgcttg ctatgaagaa
      481 aaccgataca cttgatgtcg atatctttga tatctggcac ttgtctatca caggtctctt
      541 gattaaatgg tatgctgata acaatggtgt gagtcaaggg gtttcactct ttattgctac
      601 agcagctatc gtccttgtcg gtgtgttgaa aattatcaac tctgacttga tgaaacctac
      661 atttgatgac cttcttaacg ccccaagttc atcaccaatg acatcaactc acatgaacta
      721 catgatgaac ccagttatca tggttttgga taagattttt gaaaaattct tcccaggcct
      781 tgataaatat gactttgatg ctgctaaatt gaacaagaaa atcggtttct ggggatctaa
      841 attcttcatc ggtttcatcc ttggtatcgt tatcggtatt atgggaactc cacatccaat
      901 tgcaggtgtt gcagatgcag ataaatggcg tcttgttatc aaaggatggt tgtctcttgg
      961 tttgactgcc ggtgtatctt tggaactctt ctcacttatc ggttcatggt tcatcgcagc
     1021 cgtagaacca ctatcacaag gtattacaaa cgttgctact aaacgtcttc gaggacgtaa
     1081 attcaatatc ggtcttgact ggccattcat cgctggtcgt gctgaaatct gggcttgtgc
     1141 caacgtactt gcaccaatca tgttgattga agcagtgctt ctttcaaaag ttggaaatgg
     1201 tatcttgcca cttgcaggta tcatcgctat gggtgttact ccagctctct tggttgtaac
     1261 tcgtggtaaa ttgctccgta tgattatctt cggaacactc ttgttgccac tcttccttct
     1321 ttcaggtaca cttattgcac catttgcaac agaacttgct aaaggtgtag gtgccttccc
     1381 agaaggtgtg agccaaactc aattgattac tcactctact cttgaaggac caatcgaaaa
     1441 acttcttggt tggacaattg gtaacactac aactggtgat atcaaagcaa tccttggtgc
     1501 agtagtcttc cttgtattct atatcggtat ctttgcttgg tacagaaaac aaatgatcaa
     1561 acgtaacgaa gagtacgcag caaaagcaaa ataatgcgct cctataaaat ataaactcaa
     1621 tgaaaatcaa agagcaaact aggaaactag ccgcaggttg ctaaaaacac agttttgagg
     1681 ttgtagataa
//
LOCUS       DAVAAL010000197         1643 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVAAL010000197 DAVAAL010000000
VERSION     DAVAAL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.200"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            370..>1643
                     /locus_tag="VQM60_002006"
     CDS             370..>1643
                     /locus_tag="VQM60_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HET1081820.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEKWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLK
                     GWIGNLLDNLSTAYEKFKDIERDKVIDNPKLFNLTNYLLTYSEIQKEKSKYLKGYAKT
                     NKEKYDFKKLTSAYSYLRKNNIETIGQLQTKIETLKSNSYRLNKKAKTIHKEMEDVEK
                     KILYYEIYKAKKEVYEEYQKKNIFTKEAFYNK"
BASE COUNT          784 a          214 c          269 g          376 t
ORIGIN      
        1 tagaagaaag aatagatgat aatgtagaaa aatcatctaa agatgaaata acataattgt
       61 cccttggtta caagggcgca attatacacc ctaaagggtg cttgcgtcct gcggagtcaa
      121 acccttgcag ggagcaacaa ccattcgctt tttaaaagtc aagggtaaat aaactcgcta
      181 aggctcgccc ttgactttta aaatttgaaa tgaaagtaac aattaagccg acatactgaa
      241 acaacaaaaa ttaattcagt agtcggcttt tttaattcta tcatttcaaa acgctcattc
      301 acaaagagga tataaaaaaa ctattaagcc tccacctaaa acaacaaaga aagaaaggaa
      361 gtgataaaaa tggcagacag ttttcatttt tcagtaaaca taatatcaag aggaaaaggc
      421 aaaagtgcag tagcaagtgc agcatatata agtggagaaa aaataaaaaa tgaatgggac
      481 ggagtaaccc atgactacac aagaaaagaa aaaatattag taaaaaatat aatattgcct
      541 gatcatatac caaaagaatt taatgatagg tctaccttat ggaataaagt agaaatggca
      601 gaaaaaaatt ctaatgcaca actagcaaga caattcataa taggactacc aaaagagtta
      661 tctttaagtg aaaataaaaa cctggttgaa agatatataa aagaaaattt aacatcacaa
      721 ggaatgatag tagattatgc aattcatgat gagagtcaag ataaaaatgg aaatatccat
      781 tgtcatataa tgaccataat gcgaccaata aacgaaaaag gagagttctt agccaagtca
      841 aaaaaagaat atatcctaga tgaaaaagga gaaaaagtat taaacaaaaa tggaaaacca
      901 aagacaagaa aagtagaact aacaacctgg aacgatacag gcaatgtaga aaaatggaga
      961 gaaaattttt cagatctttg taacaaatat ttagaaagag caggagcaga aaaaagagta
     1021 gaccatagga gttttaaaag acaaaattca gattatctac cgacaatcca tttaggatca
     1081 gcagcaagtg caatggaaag aaagggaata gaaactgaca agggaaacta taatagagaa
     1141 ataagaaaat ataatcaact tgtaaaaaca ataaaagaag agataaaaac attaaagggt
     1201 tggataggaa atttattaga taacttatct actgcttatg aaaaatttaa ggatatcgaa
     1261 agagataagg taatagacaa ccctaaactt tttaatctaa caaattatct attaacttat
     1321 tcagaaattc aaaaagaaaa aagtaaatat ctaaaaggat atgcaaaaac taataaagaa
     1381 aaatatgact tcaaaaagct aacaagtgca tatagttatt taagaaaaaa caacatagaa
     1441 accataggtc agttacaaac aaaaatagaa actttaaagt ctaatagtta taggctaaat
     1501 aaaaaagcaa agacaatcca taaagaaatg gaagatgtag aaaagaaaat tctatactat
     1561 gaaatctaca aagccaaaaa agaagtttat gaagaatatc aaaagaaaaa tatattcaca
     1621 aaagaagcat tttataacaa aca
//
LOCUS       DAVAAL010000198         1640 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVAAL010000198 DAVAAL010000000
VERSION     DAVAAL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.201"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            18..941
                     /locus_tag="VQM60_002007"
     CDS             18..941
                     /locus_tag="VQM60_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET1081821.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            1001..1606
                     /locus_tag="VQM60_002008"
     CDS             1001..1606
                     /locus_tag="VQM60_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET1081822.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEEFDWENL"
BASE COUNT          491 a          343 c          336 g          470 t
ORIGIN      
        1 agaaaggagt tggtagcttg actcttcgtt cacattctga acggctaatg gggactacta
       61 tcactatttc attagtggat gagcaagccg atatctttct ccaaaaatcc tttgacttgc
      121 tcaaagaact tgaataccgc ttcaacgcca atagtcaaga atctgagttg atggaaatca
      181 attatcaagc aggaatatcc cccgtcacgg ttcatccaga cctgtttgag ctgatttcac
      241 ttgggttaga gcatagccta gcgctctcta gccacctcaa tatcagcatt ggtcccttaa
      301 ttcaaacctg gcgtatcggt ttttcagatg ccaaggtcgc ccagcctcaa gaaattgaat
      361 cggtgctgcc tctaatcaat cctcatggta tcgagttaga ttcttctact tctactgtgt
      421 ttttaaaaca gaaaggaatg aaaatcgatc taggttgttt agccaaagga tacagtgcgg
      481 ataaggttgc ccaatttctt aggaaagagg gggtgacttc tgccttgatc aatctgggag
      541 ggaatatcct gaccattgga aaaaatcagg caagagggga taacccatgg caaatcggga
      601 ttcaagaccc agccaatcct aggggaaatc atttaatgac catccctgtt gtcaataaat
      661 ctgtcgtaac ttcaggcatt tatgaacgtc acctgaccgt cgatggacaa gattaccatc
      721 acatttttga cagtcaaaca ggatatcctg ttgaaacgga actagcgagt ctaacaatca
      781 tctctgataa atcagtcgat ggcgaaatct ggacgactcg actctttgga gaaagacctg
      841 cttctatcct ctggcaagtc gaaagtttgg agggcatcga agtcatcctc atcgataaag
      901 aaggccacct aagctgttct tcaggaattc ctactctata gagacaactg tttcaatgga
      961 attttaaaat tgtattatgt aaaaagagaa aggaaatctc atgttaaaac ttattgctat
     1021 tgttggaaca aattcaaaac gttctacaaa ccgtcaattg cttcaataca tgcaaaaaca
     1081 ctttgctgac aaagctgaaa ttgaacttgt tgaaatcaag gccattcctg tcttcaacaa
     1141 accagctgac aagcaagtac ctgctgaaat attggaaatt gctgctaaaa tcgaagaggc
     1201 agatggcgtt attatcggta ctcctgagta tgatcactct attccagctg ttttgatgag
     1261 cgctcttgct tggttgtctt atggtattta cccacttttg aacaaaccaa tcatgattac
     1321 aggtgcttct tacggtacgc ttggttcatc tcgtgcccaa ttgcaacttc gtcaaatctt
     1381 gaatgctcct gaaatcaagg caaatgttct tccagatgaa ttcttgctct cacactctct
     1441 tcaagcattt aacccaagtg gcgacttggt tgaccttgat gttatcaaga aattggatgc
     1501 catctttgat gacttccgta tcttcgtgaa gattactgag aaattgcgca atgcgcaaga
     1561 attgcttcgc aaagatgcag aagaatttga ctgggaaaat ttgtaagata ggagaccgaa
     1621 agaatgaaat ttgttggact
//
LOCUS       DAVAAL010000199         1607 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVAAL010000199 DAVAAL010000000
VERSION     DAVAAL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.202"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            114..1280
                     /locus_tag="VQM60_002009"
     CDS             114..1280
                     /locus_tag="VQM60_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET1081823.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
BASE COUNT          494 a          312 c          335 g          466 t
ORIGIN      
        1 aaattccagc ctggtttgct gaatggggac atgatgtcgc ccatgactgg gaatggtggc
       61 gtaaacaaat gccttatttc ctcggtaatc tctatttata aaaggagtta cctatgaatt
      121 accttgttat ttctccctac tatccacaaa actttcaaca gtttaccatc gaactagcta
      181 ataaaggcat cacagtcttg ggaattggtc aagagtctta cgagcaattg gatgagccct
      241 tgcgcaatag cttgaccgag tattttcgtg ttgataatct tgagaacata gatgaagtca
      301 aacgtgcagt tgcttttctc ttttataaac atggtccaat tgaccgcatc gagtctcaca
      361 atgaatactg gcttgagcta gacgcaacac tcagagaaca attcaatgtt tttggtgcca
      421 aaccagagga tctcaaaaag acgaaatata agtctgaaat gaagaaactt ttcaaaaaag
      481 caggtgttcc tgtggtacct ggagctgtta tcaagacgga agcagatgtt gatcaagcag
      541 tgaaagaaat cggtcttcca atgattgcca aacctgataa tggagtggga gcagccgcaa
      601 cctttaaact tgagacagaa gacgatatca atcacttcaa gcaagaatgg gaccattcaa
      661 ccctttattt ctttgaaaaa tttgtcactt ccagcgaaat ctgtaccttt gacgggctcg
      721 tggacaagga tggaaagatt gtcttctcaa caacctttga ctacgcctat acaccgcttg
      781 acctcatgat ttataagatg gacaattctt attatgtgct caaggatatg gatcctaaac
      841 tgcgcaagta tggggaagca attgtcaaag aatttggtat gaaagaacgg tttttccata
      901 ttgagttctt ccgtgagggg gacgattata ttaccatcga gtacaataac cgccctgcag
      961 gtggttttac cattgatgtt tataactttg ctcattcctt ggacctttat cgtggctatg
     1021 cagctattgt cgcaggagag gagttcccgg cgtcagactt tgaaactcag tattgtttgg
     1081 ctacttctcg ccgtgcaaat gctcactatg tttattcaga agaggatttg cttgccaaat
     1141 atagccagca gttcaaggtt aaaaaagtca tgccagctgc cttcgcggaa cttcaaggag
     1201 attacctgta tatgctgaca actccgagtc gacaagaaat ggatcagatg attgctgatt
     1261 tcggacaacg tcaagaataa gaactatcgg attaaggaaa ttcactccct taatcctttt
     1321 gttttgtctg gtaaaaaata agagcatcct aacaaggtag ctatcataaa acttgttcga
     1381 taactatttg aagcaggatt aggtggctag aaattgaatt ttaacatttc aatttagtca
     1441 tagtattgtg ttttcgtatc cttaaatcag ctaaaaggat ccatgacgac acctatacga
     1501 tatagttttc aggataccaa acaagtctat taatactcaa tgaaaatcaa agagcaaact
     1561 aggaagctag ccgcaggttg ctcaaaacac tgttttgagg ttgtgga
//
LOCUS       DAVAAL010000200         1599 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVAAL010000200 DAVAAL010000000
VERSION     DAVAAL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.203"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..164)
                     /locus_tag="VQM60_002010"
     CDS             complement(<1..164)
                     /locus_tag="VQM60_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081824.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSG"
     gene            complement(178..891)
                     /locus_tag="VQM60_002011"
     CDS             complement(178..891)
                     /locus_tag="VQM60_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET1081825.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1350..>1599)
                     /locus_tag="VQM60_002012"
     CDS             complement(1350..>1599)
                     /locus_tag="VQM60_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannose family transporter subunit IIA"
                     /protein_id="HET1081826.1"
                     /translation="IGTSFDQVDRVVSENPADTLLVFFDLGSAKMNLKMVTDFSDKSI
                     IINRVPIVEGAYNAAALLQAGAELSVIQIQLAELEINK"
BASE COUNT          527 a          306 c          255 g          511 t
ORIGIN      
        1 ccagagaaat ttttcttatc aaaaaactta aaaagagatt gataaatact atttgctaat
       61 tgacaagtgt cagatgtaaa ttcataatta acagcttcat taaaatcaac ttcagacatt
      121 tcaatgtact cttcaatagt aatattagct ttttttgtag tcattagcta atctccttta
      181 aaacgtgatc ttttcaagaa tatttttatc taaacaatcc agcaagtctt ggtaagaata
      241 gacttcgtaa gtcggctggg cttgtgtgtg attttcgagg tgatgaggat tataccagat
      301 agtgtcaatc cccgcattat tgccaccttg aatgtcggcg gttagagaat ctccaatcat
      361 cagcgtcttt tctttactaa atccagcaat ttgctggcca atcttttcat aaaaaagagc
      421 atccggcttt tgagtttgca actgttctga gataaagact tgattgaaat aaggtgctag
      481 accagattga gccaaacgtc ctgtctgaat ggcagtaatg ccatttgtcg cagcatacaa
      541 gttataatca cgctcaatga ggctgtccaa gagatcatga gcgcccgata gtgtttgtcc
      601 ctgctgggcg aggtaaaatt ggtaacgctg ggcaagaaaa ctaccgtctt tttcctgtcc
      661 aaaatgagca aataaacgag aaaagcgcgt gttaaccagc tcttgtttac tgattttctt
      721 cagctccaag tctttccaga gagccttgtt cataggaacg taataatctt tataagcctg
      781 aatatccgca actccttctt cttttagaag ttgggtcaaa gccacatcct cagcagcatc
      841 aaaatcaaga agagtgtggt cgaggtcgaa gagtagaaat ttgtagaaca atttgaggtt
      901 ttcctttctg aaaattcatt aagaacatta tatcataaag cacctcatac aattaactaa
      961 tttaatcact taaaaaaaat tcgaacactt tctatacaac tgacagctca aatctttcag
     1021 aatagaacaa tactaactat ccagcacccc atcttcataa atatatgtgt aattctagac
     1081 ctagaattcc tataaactaa atgctttcat actcttccaa gtaattgatt tccttaaatt
     1141 ttaatttttg aaggattcta aagctagaat agccccatca caatcagttt tgattgattc
     1201 acaatttaga aacactatag tttcactcct gttaaaataa aaaggaattg cataaagcaa
     1261 tccctttctg attttgaaat catttactta acattttata gttgagataa tcaatagctt
     1321 acctataaaa agagttatag taaaattcct tatttattga tttcaagctc cgctaactgt
     1381 atttgaataa ctgacagttc tgcaccagcc tgaagaagag cagctgcatt ataggcacct
     1441 tctacaattg gaaccctgtt gatgatgata cttttatcac tgaaatcagt caccattttt
     1501 aagttcattt tagcagaacc taggtcaaaa aatacaagta aagtatctgc tggattttcg
     1561 gaaacaaccc tatctacttg atcaaaactc gttccaatt
//
LOCUS       DAVAAL010000201         1574 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVAAL010000201 DAVAAL010000000
VERSION     DAVAAL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.204"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..289
                     /gene="rplL"
                     /locus_tag="VQM60_002013"
     CDS             <1..289
                     /gene="rplL"
                     /locus_tag="VQM60_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET1081827.1"
                     /translation="EEEFGVTAAAPVAVAAADAADAGAAKDSFDVELTSAGDKKVGVI
                     KVVREITGLGLKEAKELVDGAPALVKEGVATAEAEEIKAKLEEAGASVTLK"
     gene            536..>1574
                     /locus_tag="VQM60_002014"
     CDS             536..>1574
                     /locus_tag="VQM60_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET1081828.1"
                     /translation="MSLRNKIEQHIKELEGGKFQKLGDAYLSRKYNFNIVSLGSQEGT
                     DKTTKGIPDSYAVENGKYVYIMYGTHKSVISKLEGDIQSVKKKILEENIAEDKVGRLI
                     CCHTSSNITIKQKEDLEKMAEPYHLELIGINEIANDLTKIDFQYLAKEYLSISESTEQ
                     VWSINDFIRIHDESKTNAPISNDYIGDVSEIINTIKSSEKRIFLISAKPGTGKTRLAI
                     EICSLLDRNKYNIICVKSNNQDIYQDVKRNLNLHKENIVFIDDVNTTQNYISTLGLLN
                     TTSNIRFILTVRDYAKKDVINNIKVYGYNNIEPELIKDDNFKELLNQFSRNDFTNQEI
                     EHIKTISKSNPR"
BASE COUNT          590 a          208 c          286 g          490 t
ORIGIN      
        1 cgaagaagaa tttggtgtaa ctgcagctgc tcctgtagct gttgctgcag ctgatgcagc
       61 tgatgctggt gctgctaaag attcattcga cgttgaattg acatctgcag gcgacaaaaa
      121 agttggtgtt atcaaagttg tacgtgaaat cactggtctt ggtcttaaag aagctaaaga
      181 acttgttgac ggtgcaccag cacttgttaa agaaggcgtt gcaactgcag aagctgaaga
      241 aatcaaagct aaattggaag aagctggagc ttcagttact cttaaataat agagcaacta
      301 cattagtagc ttaaaaacat gattaaaccg ctattcttag gagtagcggt ttttcttttt
      361 gtttaaaaag gggcaaactt tttatatagt tgtttaggaa ataaataatt tttgtataat
      421 tggtttacta ctctaaatta acgtagtaca agagtttgtt aattaaacta taccttatgg
      481 ttttgactag tgtgatataa ttatgttgaa atacttatct ggaaaggttt ttataatgag
      541 tttaagaaat aaaattgagc aacatattaa agagctagag ggagggaagt ttcaaaaact
      601 aggtgacgcg tacctaagta gaaaatacaa ttttaatatt gtttcattag gctcacagga
      661 aggaacagat aaaacaacta aagggatccc agattcttac gctgtcgaaa atggtaaata
      721 tgtttatatt atgtatggca cccataagtc tgtaatatct aaattagaag gagacattca
      781 gtcagttaag aaaaaaattt tagaggaaaa tattgctgaa gataaagttg ggagacttat
      841 ttgttgtcat acttcttcaa atataacaat taaacaaaaa gaagatctag aaaaaatggc
      901 tgagccttat catctagaat tgattggaat taatgaaatt gctaatgatc ttacaaagat
      961 agattttcaa tatttagcaa aagagtatct atcaatcagt gaatctacag agcaagtttg
     1021 gtctatcaat gacttcataa gaattcatga tgagagtaaa actaatgctc ctatatcaaa
     1081 tgactatatt ggagatgtaa gtgaaattat aaataccatt aaatcaagtg aaaaacgaat
     1141 cttcttaata tctgctaagc ctggaacagg gaaaactaga ttagcaattg aaatttgctc
     1201 tttgctggat cgaaataaat ataacattat ttgtgtcaag agcaataatc aggatattta
     1261 ccaagatgta aaacgcaatc taaatttaca caaagaaaat attgttttta ttgatgatgt
     1321 taatactaca caaaattata tttctacact tgggctactg aacacgacta gtaatattcg
     1381 gtttattctt acggtacgtg attatgctaa aaaagatgta atcaataata ttaaagttta
     1441 tgggtacaat aatattgagc cagaacttat aaaagatgat aattttaagg aattactcaa
     1501 tcaattttct cgaaacgatt ttaccaatca agaaattgaa catataaaaa cgatatctaa
     1561 aagcaatccg cgta
//
LOCUS       DAVAAL010000202         1565 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVAAL010000202 DAVAAL010000000
VERSION     DAVAAL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.205"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(1..599)
                     /locus_tag="VQM60_002015"
                     /pseudo
     CDS             complement(1..599)
                     /locus_tag="VQM60_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546809.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(621..1445)
                     /locus_tag="VQM60_002016"
     CDS             complement(621..1445)
                     /locus_tag="VQM60_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET1081829.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFYSKESFIGYSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
BASE COUNT          470 a          353 c          292 g          450 t
ORIGIN      
        1 caaaccatct tgttcccagg ctccaagacc cgtacacagc acaatctctg cctgacggta
       61 acggtcaata aaccagccgt cgttttggtt ccaaatataa tctactggcg agttttggta
      121 aatagcatca tcgttgtagt aatcaccgac aaagaaacgt gcgtcgtaaa caccactgag
      181 agcaatcact ttggtaaaga catctggatg ttggaggaag aaattgagtg catgataggc
      241 tcccatagag caacctgtcg tcatcatgcc atcaaaccaa cctgacttgt gcttgataaa
      301 agaatggcct cctcaatcac ataacattcg taggcacggt gcatttccgc ttggtcatga
      361 gcatttttcc aagtagccaa ccagctctca ctatccaaac tagatagggt aaagaactgg
      421 acacggcctt cctcgataaa ggaagcacag gcatcaatca tgccaaaatc atagtattcg
      481 ttgtgactac cacctgatga agcaaagacc acaactggaa tcccaccatg tccataacgg
      541 ttaaggtaca tttcacggtt aggatgacca ctccagtggc taagatgttc aatatgcatt
      601 tcgttttttc ctttcttaac ttaccatttt tctgcaaaaa atctcagaca atctggtaga
      661 ttttctgacc aagggatttc actatggatg gcaccagact gaacttttag cacaagatta
      721 tccagatgta ctccccctgc tatcaaatca tggtaatagc aaagcgacga gtcgatatag
      781 gcttgtttga tattgccatc catcaaggtc ttgtctgtat catctgcttc ttctgttcct
      841 acatagatga agatgcgctg gtcaggcgat agtttctggc actcgatata gcggttaaag
      901 gcttcttggt ggagccagtt tgcagatgaa aaaacgccca agcaaccaat ttggtcttgg
      961 tattccaaac cgataaactg ggtaatattg cctcctagtg aggaaccaat catagccgta
     1021 tgctggcagt ctgcttttgt acgataggtc tcatcgataa aaggcttgac cacctccatg
     1081 acaaactcag catactccac acccttacca ccaaactgct gctctgggat aggagattct
     1141 tggaacttcc aagccgcata ctcattcatt cgccccatac catcattgtc aatagcaacg
     1201 acaatcatgc gactgatatc cggatttcgt ttgatagctg ggataatctt ccatgaatat
     1261 ccaatgaaag actctttgct ataaaaaaca ttttgcccgt catgaaagta tacaacagga
     1321 taggaacggt ctgtatcttt ctcataatct ttaggaagaa gaatacgtac acggcgctcc
     1381 ttacctgtat aaggaacctt gagtttgtgt tctttcattt ttagataaaa gtaggattga
     1441 ttcattgtca gaaaactctc tatattcaaa attttatatc attatatcat gaaaataaaa
     1501 aaaaagttag ttttttacct gtttttagaa aaaaaaacta acttttttta ctgattttcg
     1561 ctatt
//
LOCUS       DAVAAL010000203         1541 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVAAL010000203 DAVAAL010000000
VERSION     DAVAAL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.206"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..834
                     /locus_tag="VQM60_002017"
     CDS             <1..834
                     /locus_tag="VQM60_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET1081830.1"
                     /translation="GLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERKRDG
                     NSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQIIK
                     IATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSSNLL
                     NKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNECLNE
                     SISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKSQQL
                     L"
     gene            1012..1541
                     /locus_tag="VQM60_002018"
                     /pseudo
     CDS             1012..1541
                     /locus_tag="VQM60_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          562 a          234 c          292 g          453 t
ORIGIN      
        1 ggattattga acaatcatca aaacatttac ttttattcaa agtcaaaaga ttttaaattt
       61 aatacaattt ttacagagta ttcttctact acaaatatcg accaaatact agtggaacga
      121 aaacgagatg gaaactctaa aactatatat aaagttgata ataatggtaa ctatattcta
      181 gcaaaagaga aaaatggagt tcccctttca gatgtttgga atataccatt tcttaatcca
      241 aaagctaaag aaagagtagg ttatcctaca caaaaaccta ttctgttatt agaacaaatt
      301 ataaagattg ctactgataa aaatgatata gttttagacc cgttctgtgg aagtggaact
      361 actttagtag cctccaagat tttgaataga aattatatgg ggattgattt atctgaggaa
      421 gctatcaata taactcagca acgtctggaa aatgttataa aaacaagttc aaatttattg
      481 aataaaggaa tcgaagcata tagaaccaaa actgaggaag aagaaaacat tcttaaatta
      541 ttacaggcaa aaattgttca aagaaataaa ggaattgatg gttttttacc taaacatttt
      601 caaaaaaaac cgatacctat aaaaattcaa aaaaataatg aatgtctgaa tgagagtatc
      661 tctttattac agaatgctat aaactccaaa aaacttgatt ttggagtagt tataaaaact
      721 cattcagata atttattatt tgattttgat acgattcctg aaaatataat agtagttgac
      781 cattttgaac ttacaatcga aaaatggtta agtaaaagtc aacagttact atagtttgca
      841 aattatttac agaaagaaca aaaattccta ggagattatt ctggctatgt gcattgtgat
      901 atgttgcggc agtaacttag gactttagtc ctctagttct gcctatgcga tagcagtcca
      961 aggtttagga gcaaggcgac gctaagcttg gtaaactacg aaccgctaga agcttatcgt
     1021 caactggaag aagctgcact tgttggatgt tgggcgcatg tgagaaggaa gttttttgaa
     1081 gcgcccccca agcaagcgga taaatcatcg ttaggagcta aaggtttagc ttattgtgat
     1141 cagttatttg ccttggaaag agactgggag gacattgtca gctgatgaac gactacagaa
     1201 acgtcaagaa gagctccaac ccctaatgga agacttcttt gcttggtgcc gtcgtcagtc
     1261 agttttagca ggttcaaaac taggaagggc aattgaatac agcctcaagt ataaagaaac
     1321 ctttaagact attttgaaag acggacatct ggtcctttcc aataatctag ctgaatgcgc
     1381 cattaaatca ttggttatgg gacggagtaa aagagtccag tggactcttt tagcctaagc
     1441 tcagtttaaa aaagcgaggg tggttgtttt ctcaaagttt tgaaggagct aaagcaagag
     1501 ctattattat gagcttattg gaaacagcta aacgtcatca a
//
LOCUS       DAVAAL010000204         1535 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVAAL010000204 DAVAAL010000000
VERSION     DAVAAL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.207"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..152)
                     /locus_tag="VQM60_002019"
     CDS             complement(<1..152)
                     /locus_tag="VQM60_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET1081831.1"
                     /translation="MANKQDLIAKVAEAIELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGN"
     gene            complement(259..1098)
                     /locus_tag="VQM60_002020"
     CDS             complement(259..1098)
                     /locus_tag="VQM60_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET1081832.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILCHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1240..>1535
                     /locus_tag="VQM60_002021"
     CDS             1240..>1535
                     /locus_tag="VQM60_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET1081833.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLH"
BASE COUNT          454 a          333 c          227 g          521 t
ORIGIN      
        1 aagttaccaa aaccgatcaa ctgaactttt tcaccagctg caagatagtc agctactgct
       61 gcaaatacag cttcaactgc tgctgctgag tctttcttag tcaattctat agcttctgct
      121 actttagcga tcaaatcttg tttgtttgcc atgttaatga ttcctccaaa taatttctaa
      181 ttaacaaata taatcatatc ctaaaatccc ttttaggtca agttaaaaac gctatttctt
      241 cccattttat ttattttttt aggagtggta acgtatcaaa atagcccaag cgttctcacc
      301 cgtgtgagtt tgaataatgg aacccgtttc caaaacagaa attggctttt caacataagc
      361 ttgtaagctt tctttcatct cttttgccca atcatcacta ccagaatatg aaattccaat
      421 ctctgctaca gcacgttcag aaagcgatgt tatcaactca tctaaccatt ttttaaatgt
      481 tttagttcca cgacctttaa ccattggctg caattcatgg tctttcattt gcatgacaac
      541 acggatattg agaagagagc tcaacaagcc agttacacgg ctaattcgtc cacctttgac
      601 aagattttcc aaagttgaaa caccaatata aagctctgta tggtttttaa cctcttctac
      661 atgacataaa attgcctcca tatctttacc ttcttgagct aacttcgcag cctcaacaac
      721 ttggaatttc agggcttggt cagtgaagga actatcaaca acagtcacat ctgcagtaga
      781 taggctagca ccttggcgtg ctgcttctac cgtacccgaa agagcatggg acatatgaat
      841 agcaagaatc tggccaccat ctttgcatag gtcttcaaaa atctcagcaa agacacctac
      901 aggtggctga cttgttttcg gaagattctt actttcttgc atcaactgaa gaaatttacc
      961 ttcttctttc aaatccgcat cagaataaac aacattatca atcattacag ataatggaac
     1021 aattgtaata tctaattgct ttactagttc aggttcaata gtaacagatg aatcggttac
     1081 aatcttaatt tttgtcatag tatcaatctt tctattttag gattcagatt ggtttcctta
     1141 cttctaatta tatcaaaaaa aagattaaaa atcctaatgg agtcaatcaa attttccgta
     1201 aaatttgata taatcaactt ataagaaaag aggtgtccta tgattaaaaa aatttacccc
     1261 atttttacca ttttactagg tgctgctatt tatgcttttg gactgactta ttttgtagtt
     1321 ccccatcatc tctttgaagg aggggcgaca ggcattaccc tcatcacctt ttatcttttt
     1381 aaaatccctg tttccctcat gaacctgctg attaatattc cccttttcat cctagcttgg
     1441 aagatctttg gagccaaatc cctctattct agtttactag gaaccttagc tttgtccgcc
     1501 tggttagctt tttttgagca tattcccctt catat
//
LOCUS       DAVAAL010000205         1504 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVAAL010000205 DAVAAL010000000
VERSION     DAVAAL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.208"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            200..403
                     /locus_tag="VQM60_002022"
     CDS             200..403
                     /locus_tag="VQM60_002022"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081834.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(490..1077)
                     /locus_tag="VQM60_002023"
     CDS             complement(490..1077)
                     /locus_tag="VQM60_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081835.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT          531 a          286 c          252 g          435 t
ORIGIN      
        1 tcattgagta ttagaaaaca attatgttga tagagcttgg gcaagagccc aatttcagca
       61 aaaaatgaag taaatcttct cataataaaa cgcatcatat caagcacgaa aattccacga
      121 ggtcaactac agtcagaaag ctgaacaaca agccaaaacg cccaaaaaag gcggcaaaaa
      181 gcaagcacct gcaagcaacg tgccgaaatg gtcaaatcct gattatgtca acgaattaga
      241 cccaaaaatc gttgatatgc tagtagaatt tcacaagtca caaggcactt tggaaactcc
      301 cgaggcgcaa gcagaaatcg cccaaaaacg tgaagaaatc gagcaaagga gagctgagct
      361 tgagggtaaa aaacaagagc ttttgaaccg cttgaacaaa tagagtttcg caagtattat
      421 gcttacaaat tacttgagca attaactaaa atataaaccc tgcctttata tctaggcagg
      481 gtttatattt tagaaattca cgtaggttgt tacggttttt acatacccag tatagtttga
      541 gtttctatag tattcagtga taaacttcca ttttctttga gcaacatgga tataagtact
      601 tgttatgtag tatggatatg ggctttgtga atccaagtaa gactgataag cttgtatacc
      661 aaaatatgct ccaccaatta ttgcacccca tggacccccc aataaagcac ctatcctacc
      721 aatcatataa ctgattccag caccagtcat gaagttagcg aatgtgttag cttgtttatt
      781 cccatgtatt gtgttgacgt aattccaaac attaggatcg tatgatctaa aagatatatt
      841 taggtcgatt tcattctttt gataagccat ataaaatgcc ccattgatat agacgccgtc
      901 agcacgtcgt tcaatagtgt ctacacttcc atctggattg acaacctcaa gaacttcatc
      961 gcttaaaata tttacttgcg tatctccgaa ccgcactgat gagccattct caaactgagc
     1021 ctcaccagat acaactttag agtttgccga taagctatca tcagcaaaaa caaacaagcg
     1081 acggggaaat gctagacata cagaaaacag acataactag caaacacatg catttaaaca
     1141 tcttagacat aacggaaact cctttgtatt tttgattttt ttcaactttt attatacaat
     1201 aaaaccaaat aaaaagaaag cggtaacaat atgcttaatg cgaaaatttt ttatatattt
     1261 ttatgtttga tcgttatcga aactacaggc ttgttgttgt tgaaaagagg tctcgaaatg
     1321 ggttatttag acacagaagc tattatcctc gcagtttttt catttgcttt ttacaaccta
     1381 tgttcattcg cttgggtctg ctctacaata aaaaacaata aaaaataaat agacgtattt
     1441 tcaaaaaaaa aaaaaatgca tatttatctt agcaaaacga cgatttaaat cgtcgttttt
     1501 ttgt
//
LOCUS       DAVAAL010000206         1473 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVAAL010000206 DAVAAL010000000
VERSION     DAVAAL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.209"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(571..1440)
                     /locus_tag="VQM60_002024"
     CDS             complement(571..1440)
                     /locus_tag="VQM60_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET1081836.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
BASE COUNT          478 a          312 c          287 g          396 t
ORIGIN      
        1 tactatacaa cctcaaaact gtgttttgaa caacctgcgg ctagcttctt agtttgccct
       61 ttgattttca ttgagtataa aattgcagat aaaacgataa caatcatagg actcagcaga
      121 gtcaatgtag gtcgaagaat agccccccta atcgcttatt ttatagcaaa atgaaataga
      181 aactgaacat tttgttgagg aaggtaaatc aattcctcac aatgtttgag aaagaagggg
      241 ggactattcc aacttcaacc cactataaag cagacttaca agatagggaa cccatctccg
      301 ataatatata gtagaatgaa aactgcacaa tttgattgga gaattcaaac caatttctaa
      361 caatgtttta gaaacaaggg tctattattt caactgcaac ctactatagc ccattgaagc
      421 tggaatagcc cactgagact tctaaaccac tgctggagag gagtttgttt agagtttctt
      481 tcatttccct aaaaaatcaa aaagagagaa ccagtctgat cctctcttac ttatagatgg
      541 tgctcactca aatgttaaag gagctatttc ttagagataa aggaatttga atacagctac
      601 tgccgcaatt gctgctgcga taggtgctac tactggtacc caagaatacc accattttga
      661 atcgccttta tgctcaccaa gaactgattt tggaaggaaa gcatgaagga gacgtggtcc
      721 caagtcacgg gctgggttca aggcaggtcc tgtaggtcct ccaagtgatg ttaccaaagc
      781 cataacgagg aatccaagtg ccaagtgagc cacagaaagt cctgaagcag tgtgtggagc
      841 cacctgtgct ttaatagcca agtcagaaaa atcaactgtt tgtcctgctt ctattgcctt
      901 ttgtttcatg aattgaagca cttcagcacc aaagaagttt ttagtcaaac caagagctgc
      961 aaagaacaaa acaaatgaac caacaaactc attaatcaaa ccattgacag ttgctgcata
     1021 gcgactttct tttgtaccat ggtcaatact tgaaatagtt gagaaagttc ccaagatgtt
     1081 atttggtttt tcagttttca agtagaatgg acggtatgtt gccacaacta aggcttggcc
     1141 aaagatagcc cccaagactt gcgcgataat gtaaggtacc acttgtgccc aagggaaaag
     1201 accgctaact gcaagcccta gagtgaaagc agggttgatg tgattcccag atacgttacc
     1261 aaacatcaag gctgggatca taacccccat accataacca acagcgatga cgatccagcc
     1321 actttggtga cctttcgtac ctttaagttc aacgttggca actgcaccat tcccaagaat
     1381 gatcaaaatg gcagttccca aaaattcagt ggcatacttc agtgcccatg tgaaatccat
     1441 ttgctagatt ctccttaaaa ttttttagat att
//
LOCUS       DAVAAL010000207         1456 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVAAL010000207 DAVAAL010000000
VERSION     DAVAAL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.210"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            70..>1456
                     /gene="pcpA"
                     /locus_tag="VQM60_002025"
     CDS             70..>1456
                     /gene="pcpA"
                     /locus_tag="VQM60_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HET1081837.1"
                     /translation="MKKTTILSLTTVAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGV"
BASE COUNT          573 a          199 c          231 g          453 t
ORIGIN      
        1 ttttataaca acggctagca aagttataat tttatatcta ttagaaaata aatatgtaag
       61 gaaatttaaa tgaaaaaaac tacaatatta tcattaacta cagttgcggt tattttagca
      121 gcatatgtcc ctaatgaacc aatcctagca gatactccta gttcggaagt aatcaaagag
      181 actaaagttg gaagtattat tcaacaaaat aatatcaaat ataaggttct aactgtagaa
      241 ggtaacatag gaactgttca agtgggtaat ggagttactc ctgtagagtt tgaagctggt
      301 caagatggaa aaccattcac gattcctaca aaaatcacag taggtgataa agtatttacc
      361 gttactgaag tagctagtca agcttttagt tattatccag atgaaacagg tagaattgtc
      421 tactatccta gctctattac tatcccatca agcataaaaa aaatacaaaa aaaaggcttc
      481 catggaagta aagctaaaac tattattttt gacaaaggca gtcagctgga gaaaattgaa
      541 gatagagctt ttgatttttc tgaattagaa gagattgaat tgcctgcatc tctagaatat
      601 attggaacaa gtgcattttc ttttagtcaa aaattgaaaa agctaacctt ttcctcaagt
      661 tcaaaattag aattaatatc acatgaggct tttgctaatt tatcaaattt agagaaacta
      721 acattaccaa aatcggttaa aacattagga agtaatctat ttagactcac tactagctta
      781 aaacatgttg atgttgaaga aggaaatgaa tcgtttgcct cagttgatgg tgttttgttt
      841 tcaaaagata aaactcaatt aatttattat ccaagtcaaa aaaatgacga aagttataaa
      901 acgcctaagg agacaaaaga acttgcatca tattcgttta ataaaaattc ttacttgaaa
      961 aaactcgaat tgaatgaagg tttagaaaaa atcggtactt ttgcatttgc ggatgcgatt
     1021 aaacttgaag aaattagctt accaaatagt ttagaaacta ttgaacgttt agccttttac
     1081 ggtaatttag aattaaaaga acttatatta ccagataatg ttaaaaattt tggtaaacac
     1141 gttatgaacg gtttaccaaa attaaaaagt ttaacaattg gtaataatat caactcattg
     1201 ccgtccttct tcctaagtgg cgtcttagat tcattaaagg aaattcatat taagaataaa
     1261 agtacagagt tttctgtgaa aaaagataca tttgcaattc ctgaaactgt taagttctat
     1321 gtaacatcag aacatataaa agatgttctt aaatcaaatt tatctactag taatgatatc
     1381 attgttgaaa aagtagataa tataaaacaa gaaactgatg tagctaaacc taaaaagaat
     1441 tctaatcagg gagtag
//
LOCUS       DAVAAL010000208         1438 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVAAL010000208 DAVAAL010000000
VERSION     DAVAAL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.211"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            256..1266
                     /gene="ccpA"
                     /locus_tag="VQM60_002026"
     CDS             256..1266
                     /gene="ccpA"
                     /locus_tag="VQM60_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET1081838.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
BASE COUNT          446 a          235 c          336 g          421 t
ORIGIN      
        1 ctttaggagg gcttagtttc ctcctttttg tttagtctaa ggtggtagca aggagctaaa
       61 cctaggtttt ctatgaaatt ttttcaatca atcttcaaaa aacacttgaa agtgtttaca
      121 attagtgata gaatggaata agtagaaata tgaaaacgaa attttcacat cttcactttt
      181 gtaagtagat atagagcttc aatcagtacc tctacttgta aaagaatgtg tgagatagaa
      241 aggacaggaa aaggaatgaa tgcagatgat acagtaacca tttatgatgt cgctcgtgaa
      301 gcaggtgttt ccatggcgac ggtcagccgt gtggtcaatg gcaataaaaa tgtaaaagag
      361 aatacccgta aaaaagtgct agaggtaatt gatcgcttgg attatcgtcc aaatgcagtt
      421 gcgcgtggtc ttgcaagtaa aaagacaacc actgtcggtg tcgtgattcc aaatattacc
      481 aatggttatt tttcgagttt ggctaagggg attgatgata ttgcagaaat gtacaagtac
      541 aatattgtcc tagctaatag cgatgaagat aacgagaaag aagtttctgt tgtcaatacc
      601 ctcttttcaa agcaggtaga tggcattatc tatatgggtt atcacttgac agataaaatt
      661 cgctcagaat tttcgcgttc acgtactccg attgttctcg caggaactgt cgatgttgag
      721 caccagttgc caagtgtcaa tattgactat aagcaagcaa caattgatgc agtgagttac
      781 cttgctaaag aaaatgagcg tattgctttc gttagcggtc cgctagtgga tgacatcaat
      841 ggtaaggttc gtttagttgg ctacaaggaa accttgaaaa aagcaggaat cgcttatagt
      901 gagggtttgg tatttgaatc taaatatagc tatgatgatg gttacgcctt agcagagcgt
      961 ttgatttcat caaatgcaac tgcagcagtt gtgacaggtg atgagttggc agcaggagtc
     1021 ttgaacggtt tggctgataa gggtgtttct gtgccagaag attttgaaat tattactagt
     1081 gatgattcac aaatctcacg ctttacccgt ccaaacttga caacgattgc ccaacctctt
     1141 tatgaccttg gtgccattag tatgcgtatg ttgaccaaga ttatgcataa ggaagagttg
     1201 gaagaacgtg aagttctctt acctcatggt ttgacagaac gtagctcaac acgaaaacgt
     1261 aaatagaaaa aatcagggaa tcgagaagat ttcctgattt tgtgctctta aacaagagat
     1321 gttgacttaa tgaataccat agagcggact aggcggctgg ttaaaggctg cgcaacacac
     1381 tgttttgagg ttgtagatag aactgacgaa gtcagctcaa agcactgctt tgaggttg
//
LOCUS       DAVAAL010000209         1433 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVAAL010000209 DAVAAL010000000
VERSION     DAVAAL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.212"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            178..1380
                     /gene="rpsA"
                     /locus_tag="VQM60_002027"
     CDS             178..1380
                     /gene="rpsA"
                     /locus_tag="VQM60_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET1081839.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
BASE COUNT          452 a          229 c          328 g          424 t
ORIGIN      
        1 aatccagcta ccccagttct taggtaataa tcaagataaa aagcaaaata tcttagggta
       61 ttttattttt ataattgaaa gacgtgaatg atatgaacat gtccttgcgg gtgcttagga
      121 aaaaaattat aagtatgtca agtttaagaa aaacttgatt gttggaggat tttttagatg
      181 aacgaatttg aagatttgct aaatagcgtt agtcaagttg agactggtga tgttgttagt
      241 gctgaagtat tgacagttga tgcgactcaa gctaacgttg caatctctgg aactggtgtt
      301 gaaggtgtct tgactcttcg cgaattgaca aacgatcgtg atgcagatat caatgacttt
      361 gttaaagtag gagaagtatt ggatgttctt gtacttcgtc aagtagttgg taaagatact
      421 gatacagtta cataccttgt atctaaaaaa cgccttgaag ctcgcaaagc atgggacaaa
      481 cttgttggtc gcgaagaaga agttgttact gttaaaggaa cgcgtgccgt taaaggtgga
      541 ctttcagtag aatttgaagg tgttcgtgga tttatcccag cttcaatgtt ggatactcgt
      601 ttcgtacgta acgctgagcg ttttgtaggt caagaatttg atactaaaat caaagaagtt
      661 aacgctaaag aaaaccgttt catcctttca cgtcgtgaag ttgttgaagc agctactgca
      721 gcagctcgcg ctgaagtatt cggtaaattg gctgttggtg atgttgtaac tggtaaagtt
      781 gctcgtatca caagcttcgg cgctttcatc gaccttggtg gtgttgacgg attggttcac
      841 ttgactgaat tgtcacatga acgtaacgta tcaccaaaat cagttgtaac tgttggtgaa
      901 gaaattgaag tgaaaatcct tgatcttaac gaagaagaag gacgtgtatc actttcactt
      961 aaagcaacag taccaggacc atgggatggc gttgagcaaa aattggctaa aggtgatgta
     1021 gtagaaggaa cagttaaacg tttgactgac ttcggtgcat ttgttgaagt attgccaggt
     1081 atcgatggac ttgttcacgt atcacaaatt tcacacaaac ggattgaaaa tccaaaagaa
     1141 gctcttaaag ttggtcaaga agttcaagtt aaagttcttg aagttaacgc agatgcagaa
     1201 cgcgtgtcac tttctattaa agctcttgaa gaacgtccag cccaagaaga aggacaaaaa
     1261 gaagaaaaac gtgctgctcg tccacgtcgt ccaagacgtc aagaaaagcg tgatttcgaa
     1321 cttccagaaa cacaaacagg attttcaatg gctgatttgt ttggtgatat cgaactttaa
     1381 tcaaattgaa aattcacaaa atcctttgtt tactaaacaa gggatttttc ttt
//
LOCUS       DAVAAL010000210         1413 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVAAL010000210 DAVAAL010000000
VERSION     DAVAAL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.213"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(13..807)
                     /locus_tag="VQM60_002028"
     CDS             complement(13..807)
                     /locus_tag="VQM60_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET1081840.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            complement(807..>1413)
                     /locus_tag="VQM60_002029"
     CDS             complement(807..>1413)
                     /locus_tag="VQM60_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET1081841.1"
                     /translation="TSSLILFLLAKKDGFSAARLIILGIGFQAVTRAGTSFLLLKVAR
                     YELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFNKRLEIIELGEEIAIGLGA
                     NPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALNIGKKRSPILAGLVGILLV
                     LLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRNI"
BASE COUNT          454 a          236 c          226 g          497 t
ORIGIN      
        1 aatattcaat tattaagagc taacatggtg cttgccaata ggaatcatta gaggcgaatt
       61 ggaaataggg tcacgtataa tttttgcttc aagattaaag atatctttaa ctagtttatc
      121 atttagtata tcttcaggct ttccctctgc aacaagttta ccttctttaa ttgcaaatag
      181 gtaatcagcg tatcttgctg ttagatttat atcgtgcaaa atcatgcaaa tggttgtctt
      241 atatttttgg tttagatcag tcaagaggtc taatagttct atttgatatg agatatccaa
      301 gtaagtagtt ggctcatcta aaagtaggat acttgtatct tgggctaggg ctagagctat
      361 ccatactctt tgcctttgac ccccagaaag ttcttcaact aggttatttg ctagatcttc
      421 aacattggcc ttaaccattg atctgtttat tatttcaagg tcatcttttc caagactctt
      481 aaaaggcttt ctgtagggga aacgaccacg gcttacaaga tcagctactg ttattgattc
      541 agggattatt ggagattgag gtaatatagc tatgtgtttt gctaaatctt tttctttata
      601 agaattaatt gatttattat cgagcaatac ttctccctct aatggcttta taagtcgaga
      661 caaggtttta atgagtgttg atttcccaca accatttgac ccaataataa ctgatatttt
      721 ttcttcaggt atttttatat ttatattttc caagattatt ttttcatcat aaccgcaggt
      781 aagattattt gaccacagac ctttcattat atattcctcc tgttcatttt tattagtaag
      841 tatattaagt atggtgaacc taacaagcca gttacaacac ctactggata tctagctggt
      901 aaaatatttt gagagaatat gtctgataac aaaactagta aaattccaac caatccagct
      961 aatattgggc ttcttttctt gccaatattt aaggctatgg gaccagctaa aaaagatata
     1021 caagctattg gtcctgtaat tgaagtagaa aaagcagtta aagatacagc gcaaaaaatt
     1081 aaaacaagcc ttgaaagctc gggatttgct ccaagtccga ttgctatttc ttcaccaagt
     1141 tcaataattt ctagtctttt attaaaaaat aaaactaata tagtagcaat aatacttact
     1201 attagaacaa gaggtatgtc atctaacttt gtaaaagata aagagccact gagccatctc
     1261 ataacttctt gtaattcata tcttgctact ttcaacaata aaaatgaggt gcctgctctt
     1321 gtgacagctt gaaaaccaat acctaatatt atcagtcttg ctgctgaaaa accatctttt
     1381 ttagctagta aaaataatat taaagatgat gtt
//
LOCUS       DAVAAL010000211         1404 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVAAL010000211 DAVAAL010000000
VERSION     DAVAAL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.214"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            142..493
                     /locus_tag="VQM60_002030"
                     /pseudo
     CDS             142..493
                     /locus_tag="VQM60_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            769..>1404
                     /locus_tag="VQM60_002031"
     CDS             769..>1404
                     /locus_tag="VQM60_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET1081842.1"
                     /translation="MEQLKNTTDLLGLEDKNIKILSVLKYQTHLVVQAKLDSPAPPCP
                     HCQGKMIKYDFQKASKIPLLDCQGLPTVLHLKKRRFQCKNCLKVVVSQTSIVKKNCQI
                     SNMVRQKIAQLLLEKQSMTEIAHRLAVSTSTVIRKLREFKFETDWTKLPKVMSWDEYS
                     FKKSKMSFIAQDFESKSILAILDGRTHAVIRNHFQRYQREVRELVEVITMDM"
BASE COUNT          433 a          302 c          281 g          388 t
ORIGIN      
        1 tcgaatatga tcgagccttg agcaggcagt tagtctctct ggaagaagat ataattattt
       61 gaattaagat cgagacaacg cacaccagag attgcgatac tgttatagaa gtactaatgc
      121 ccttttttgt ttcaatatac tatggctccg atgacctata aagatacgat gacgagtgac
      181 tttttcgaag cttgcttcca aaaattctta ctacctactt tagatacacc atcccttatc
      241 attatggacc atgcaaggtt tcacagaatg aacatgtgta aggagcaggg catagactgt
      301 taccacttcc tacctattca cccgagtata atcccattga gaaaatatgg gcttacatca
      361 aaaacatctc agaataatat tgtcaaatta cgatgctttt cttgaggcac ttttgtccta
      421 ttcttgtttc agccgactat actccgttat tgggcagcta cggaacagtc gatgggacga
      481 tggggggaca taaaaaaatc ctccagtttt gttttttata acagtatact ggagaattga
      541 caatctcggt agatacctcg ttatagcgcg gttacttatt aggcagttac aaaacaactg
      601 tgaacagaaa acattccaga gtcagacaag actttggaat gctttggctc tataatttct
      661 gtagtgggta atcccacccc aggaattata gggtcgtttc ttgtagaaaa aaagccccat
      721 atgacctata atgaaaagcg tctaaccaac tcattagaaa gggttcatat ggaacaactt
      781 aagaatacca cagatttgct cggattggaa gacaaaaata tcaaaatctt gtctgttctg
      841 aaataccaaa cccatctagt cgttcaggca aagttggatt cccccgctcc tccttgtcct
      901 cattgtcaag ggaagatgat caaatacgac ttccagaaag cctctaaaat tccgcttctc
      961 gactgtcagg gtttacccac ggtactgcat ctcaaaaagc gccgctttca gtgcaagaat
     1021 tgccttaagg tggtcgtttc tcaaacatcc attgtcaaga aaaattgcca gatttccaac
     1081 atggtgagac aaaaaatcgc tcagctcctc cttgaaaagc agtctatgac tgagattgcc
     1141 cacagattgg cggtctcaac ttccaccgtc atccgaaaac tgagggaatt taagtttgaa
     1201 accgattgga ccaagttgcc aaaagttatg agttgggatg agtatagctt caaaaagagc
     1261 aaaatgagct tcattgccca agattttgag tccaaatcca tcctcgcaat tttagacggg
     1321 cgaactcatg cggtgattcg aaaccatttc caacgctatc agagagaggt tcgggagctg
     1381 gtcgaggtca tcaccatgga catg
//
LOCUS       DAVAAL010000212         1377 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVAAL010000212 DAVAAL010000000
VERSION     DAVAAL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.215"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            243..923
                     /locus_tag="VQM60_002032"
     CDS             243..923
                     /locus_tag="VQM60_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET1081843.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANIIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFG
                     NPSTLSRK"
     gene            883..>1377
                     /locus_tag="VQM60_002033"
     CDS             883..>1377
                     /locus_tag="VQM60_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET1081844.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTS"
BASE COUNT          347 a          307 c          266 g          457 t
ORIGIN      
        1 cttctgcatt gcttgttttg tatgacataa aatgccctcc ttgataagta agcgtttact
       61 ttgtatatta caaaaatatc ttaactcttt ttcaaaactt ttcaaatatt ttgtttggaa
      121 atttcagaaa ttttatgtct atggtaaaaa atccttataa cggcaataaa aaatagatat
      181 tatccaaaga agattttaag tgctacaata actgtattat ttctagatgg gaggttctat
      241 ttttggattg gtccattgtt gaacaatatc taccactata tcaaaaggca ttctttctga
      301 ccttgcatat tgcagtttgg ggaattttgg gatcctttct gctcggttta atcgttagta
      361 tcatccgaca ttatcgaatc cctgttttgg cgcaagtagc gacagcctac attgaattgt
      421 cacgtaatac gccccttttg attcaactct tctttctcta cttcggtctt ccccgaatcg
      481 ggattgtcct atcttcagaa gtctgtgcaa cgcttgggct tgtcttttta ggaggctcct
      541 atatggcaga atctttccga agtgggctgg aagccatcag tcaaacccag caggagattg
      601 gcctcgctat tggtctgaca cctctacagg tctttcgcta tgtggttctt ccgcaagcaa
      661 cagcggtggc actcccctcc tttagtgcca atatcatttt ccttatcaag gaaacctctg
      721 ttttctcagc agtggctttg gccgacctca tgtatgtcgc caaggatttg attggtctct
      781 actatgagac agacattgcg ctagctatgt tggtagttgc ttatctgatc atgctgctac
      841 ccatctcact ggtctttagc tggatagaaa ggaggctccg ccatgcagga ttcgggaatc
      901 caagtactct ttcaaggaaa taatctcctg agaatcttac agggattggg cgttacgatt
      961 gggatatcca tcctgtctgt cctcttatcc atgatgttcg gaacagtcat gggaatcatc
     1021 atgacctccc attctagaat catacgattt ttaacacgat tgtatctgga atttatccgt
     1081 atcatgcccc agctggtgct actcttcatc gtttactttg gcttggctcg aaactttaat
     1141 atcaatatct caggtgagac ttcagctatt atcgttttta ccctctgggg aacagctgaa
     1201 atgggagact tggtacgtgg agctatcact tctctcccta aacatcagtt tgaaagtgga
     1261 caggcactcg gcttgactaa tgttcaactt tactaccaca tcatcatccc acaagtctta
     1321 agaagactgc taccgcaggc tatcaatctt gtcactcgga tgattaaaac cacttca
//
LOCUS       DAVAAL010000213         1320 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVAAL010000213 DAVAAL010000000
VERSION     DAVAAL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.216"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..1219
                     /gene="phtE"
                     /locus_tag="VQM60_002034"
     CDS             <1..1219
                     /gene="phtE"
                     /locus_tag="VQM60_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET1081845.1"
                     /translation="LGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPG
                     QTFKYTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKG
                     TDALVRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQ
                     STYIVEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETG
                     NSTSNSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMA
                     DFTGEAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGM
                     LNPEGNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPS
                     GEVIKKNLSDLIA"
BASE COUNT          528 a          190 c          258 g          344 t
ORIGIN      
        1 attaggtatc aatatgctag taaaattaat aacaccagat ggaaaagtat tggagaaagt
       61 atctggtaaa gtatttggag aaggagtagg gaatattgca aactttgaat tagatcaacc
      121 ttatttacca ggacaaacat ttaagtatac tatcgcttca aaagattatc cagaagtaag
      181 ttatgatggt acatttacag ttccaacctc tttagcttac aaaatggcca gtcaaacgat
      241 tttctatcct ttccatgcag gggatactta tttaagagtg aaccctcaat ttgcagtgcc
      301 taaaggaact gatgctttag tcagagtgtt tgatgaattt catggaaatg cttatttaga
      361 aaataactat aaagttggtg aaatcaaatt accgattccg aaattaaacc aaggaacaac
      421 cagaacggcc ggaaataaaa ttcctgtaac cttcatggca aatgcttatt tggacaatca
      481 atcgacttat attgtggaag tacctatctt ggaaaaagaa aatcaaactg ataaaccaag
      541 tattctacca caatttaaaa ggaataaagc acaagaaaac tcaaaacttg atgaaaaggt
      601 agaagaacca aagactagtg agaaggtaga aaaagaaaaa ctttctgaaa ctgggaatag
      661 tactagtaat tcaacgttag aagaagttcc tacagtggat cctgtacaag aaaaagtagc
      721 aaaatttgct gaaagttatg ggatgaagct agaaaatgtc ttgtttaata tggacggaac
      781 aattgaatta tatttaccat cgggagaagt cattaaaaag aatatggcag attttacagg
      841 agaagcacct caaggaaatg gtgaaaataa accatctgaa aatggaaaag tatctactgg
      901 aacagttgag aaccaaccaa cagaaaataa accagcagat tctttaccag aggcaccaaa
      961 cgaaaaacct gtaaaaccag aaaactcaac ggataatgga atgttgaatc cagaagggaa
     1021 tgtggggagt gaccctatgt tagatccagc attagaggaa gctccagcag tagatcctgt
     1081 acaagaaaaa ttagaaaaat ttacagctag ttacggatta ggcttagata gtgttatatt
     1141 caatatggat ggaacgattg aattaagatt gccaagtgga gaagtgataa aaaagaattt
     1201 atctgatctc atagcgtaag gaatagcagt agaaaaagtc tgaatcaaaa atgaagttct
     1261 ctcaaaagtt agaaataaaa ctctgacttt gggagaattt cattttatta ttaatatata
//
LOCUS       DAVAAL010000214         1223 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVAAL010000214 DAVAAL010000000
VERSION     DAVAAL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.217"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..>1223
                     /locus_tag="VQM60_002035"
     CDS             <1..>1223
                     /locus_tag="VQM60_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET1081846.1"
                     /translation="QNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEEADVSNQ
                     LTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQKLLADYQ
                     AIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVKSVLQEK
                     DRLGGIIGAVSEHLTFDMHYQTALEIALGASSQYIIVEDENAATKAIDFLKRNRAGRA
                     TFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATTAIFDTV
                     EHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQKEIAEEE
                     ASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQVEELETL
                     WKLQEEEIDRLSEGD"
BASE COUNT          393 a          242 c          292 g          296 t
ORIGIN      
        1 ccaaaataat caaaaactca atcgtttaga agctgaattg ctggctttct cagacgatcc
       61 tgatcagatg attgagctct tacgtgaacg ctttgtagct cttttacaag aagaagcgga
      121 tgtctcaaac cagttgaccc gtattgagaa tgagttggaa aatagtcgtc agctttctca
      181 aaaacaagca gatcaactag aaaagctgaa agagcaatta gctatagcta aagagaaggc
      241 tagtcagcaa aaggaagagc ttgaaactgc caaggagcag gttcagaaat tattggctga
      301 ctaccaagct attgccaagg agcaagagga gcagaaaact tcctatcaag ctcaacaaag
      361 ccaactcttt gaccgtctgg ataatctcaa aaacaagcag gccagagctc aaagtttgga
      421 aaatatcctg agaaatcata gtaactttta tgcaggtgtt aagagtgttc tccaagaaaa
      481 agatcgccta ggtgggatta ttggtgcagt cagtgagcat ctgacctttg atatgcatta
      541 tcaaactgcc ctagaaattg cgcttggagc gagcagtcag tacattattg tagaagatga
      601 gaatgcggca accaaagcca ttgatttcct caaacgaaac agagccggtc gtgcaacctt
      661 tcttcctttg accactatta aggcgcgtac gatttctagt cagaaccaag atgctatcgc
      721 tgtaagccca ggtttccttg ggatggcaga tgagttggtg acttttgata ctagactgga
      781 agccattttc aagaacttgc tagctacgac ggctatcttt gataccgtag aacatgcgcg
      841 tgcagcagct cgccaagttc gttatcaggt tcgtatggtg acattggatg ggacagaatt
      901 acgcacgggt ggttcttacg caggtggagc caatcgtcag aacaacagta ttttcatcaa
      961 gccagaactg gagcaattac aaaaagaaat tgctgaagaa gaagcaagtt tgcgttcaga
     1021 tgaagtgagt ttgaagaact tgcaagatga gctagctaga ttgacagaaa gattagaagc
     1081 catcaaatct cagggagagc aggctcgtat tcaggagcaa ggcttgtccc tcgcctatca
     1141 gcagaccagt cagcaagttg aagaactgga aactctttgg aaactccaag aagaggaaat
     1201 agatcgtctt tccgagggag att
//
LOCUS       DAVAAL010000215         1217 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVAAL010000215 DAVAAL010000000
VERSION     DAVAAL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.218"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..164
                     /locus_tag="VQM60_002036"
     CDS             <1..164
                     /locus_tag="VQM60_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET1081847.1"
                     /translation="YNHALSKERSKVENIFAKVETFKMFSTTYRNHRKRFGLRMNLIA
                     GIINHELGF"
     gene            complement(902..1162)
                     /locus_tag="VQM60_002037"
     CDS             complement(902..1162)
                     /locus_tag="VQM60_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081848.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELELVKSTILQNDDISLDQNEIFEILKNFFDG"
BASE COUNT          415 a          227 c          197 g          378 t
ORIGIN      
        1 cctataacca tgcgctatct aaggagagaa gcaaggttga gaacatcttt gccaaagtag
       61 aaacgtttaa aatgttttca acaacctatc gaaatcatcg taaacgcttt ggattacgaa
      121 tgaatttgat tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta
      181 ttatatatgc acagacatat gaaatctttt tgagatagca gttatgatat atttatccat
      241 tcacaacatc ttctgtacca atactacaat attggtacat tttgattaca caatcgtttc
      301 aaaaaatatg atggaagcct gagtatttta tagtaactcc cttcctatca actagatcaa
      361 ccagttgagt gttgaatgtt gaaacaccct agcctcagca aaggactatg agcagcattt
      421 cctagccaat aggacgctcc aaatcaccta ttctaaatcc atcactagct tggtcagtaa
      481 gatacagact gcaaggcaag actgacgtgg ttgatgagat tttggaagag tattacaatc
      541 atagattcac cagtagactt gaccgcagat ttaactatag tagattgaaa ctagaatagt
      601 acacttctgc ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatc aatttgtcct
      661 gttctcgttt cattttacta tactaaaaaa ccacctgaaa caaatcaagt gactagagga
      721 taaaaacaaa aactgggcaa aaagttttga aaagcaaaaa acgcataata tcaggtcttg
      781 aaaaaccttg atactatacg tttgattgtg ggaagattga cttcattttc tcctgaaatt
      841 gaagtttttt ctagtttcca gatatagacg aattcaatat agccatctcg ctacaattat
      901 tttaaccatc gaaaaaattt ttcagaattt caaaaatttc attttgatct aatgaaatgt
      961 catcattttg aagaatagtt gacttcacaa gctctaattc tatcacaaag cggtgcatgt
     1021 ctgcttttac cgcactactt gatgttatag ccgataactt ttcatctcct agcaaggaag
     1081 ctgtcaaacg ggtaaggtca ttcaaatgct ttttaatgga cttacttaaa ccttgagcac
     1141 ctgtagcaga gcgttccctc atatccagcc aagctttagc cttaaaaata attaatactc
     1201 aatgaaaatc aaagagc
//
LOCUS       DAVAAL010000216         1191 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVAAL010000216 DAVAAL010000000
VERSION     DAVAAL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.219"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            1..919
                     /locus_tag="VQM60_002038"
                     /pseudo
     CDS             1..919
                     /locus_tag="VQM60_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          369 a          250 c          200 g          372 t
ORIGIN      
        1 caacttcaac tgttattcgt aagctcaatg attttcactt taaacatgat ttttcgcgtc
       61 ttcctgagat tatgtcttgg gatgagtatg cttttacaaa gggaaagatg agcttcattg
      121 cgcaagattt taacaatctc aatatcatca ctgttcttga aggtagaaca caagctgtca
      181 tccgagatca ctttcttaaa tatgatagag ccgtccgatg tcgcgtcaaa atcattacta
      241 tggatatgtt tagcccttac tatgacttag ctaaacagct tcgctttcaa atttctaggc
      301 tcaggctgaa acagtctcct agactgtttc actcccgaat gctaaaatcg ttcttgatcg
      361 ctttcatatt gtacaacatc ttagccgtgc tatgagtcgt gtgcgtgttc aaatcatgaa
      421 tcagtttcat cgaaaatccc atgaatacaa ggctatcaag cgctactgga agctcataca
      481 acaggatagt cgtaaactca gcgataaaca tttttatcgc cctacttttc gtatgcattt
      541 aaccaataaa gagattttag acaagctttt gagctattca caagacttga aacatcacta
      601 tcagctctat caactcttgc tgtttcactt tcagaataag gaaccggaga aatttttcgg
      661 actcattgag gacaatctaa agcaggttca tcctcttttt cagactgtct ttaaaacctt
      721 tctaaaggac aaagagaaaa tcgtcaacgc ccttcaacta ccctattcaa acgccaaatt
      781 ggaagcgacc aataatctca tcaaacttat caagcgcaat gcctttggtt ttcggaactt
      841 tgaaaacttc aaaaaacgga ttttcatcgc tctgaacatc aaaaaagaaa ggacgaaatt
      901 tgtcctttct caagcttagc tgacttcaac ccactacagt tgacaaagag tcaaaaaaac
      961 tccgtagtct ctagcgctat aaatccggct agagattaca gagttatttt tttatctaaa
     1021 cttcctacaa gggtatatta tctactgaaa ggatatggta tttgtaggga atactttaaa
     1081 gagttcttac aggaagtcag tattaactag ttatattagg tttagtttac ccattcacca
     1141 ttggcattga ctctatagct atctacagtt gtgttgactg caagggcacc t
//
LOCUS       DAVAAL010000217         1189 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVAAL010000217 DAVAAL010000000
VERSION     DAVAAL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.220"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            185..497
                     /locus_tag="VQM60_002039"
                     /pseudo
     CDS             185..497
                     /locus_tag="VQM60_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            501..>1189
                     /locus_tag="VQM60_002040"
     CDS             501..>1189
                     /locus_tag="VQM60_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET1081849.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASL"
BASE COUNT          432 a          173 c          226 g          358 t
ORIGIN      
        1 aaatcgtgca tcagttagtt gtttacttgc ttcataattc atagaactac tataccatat
       61 tttgtttcgc aggaagtcta atgctaaaat cttatcagtg agaagatttc ttaaagttta
      121 ttctttgtag tttcatctgt attagtacat aatttaactt agagaaaata ggaggactaa
      181 tcgaatggaa ttaaaagatt ttacagaaaa agaacaggaa atgattaaga aaaggcttac
      241 aatgtctaat attagtgaca aagaaactac tgagaagatt ctggcgctag tcccacaaga
      301 cttgattaag cgaatcccgt tttttgtcag aaaacatgct acaacacgta cgattaaacg
      361 catttcaatt gaataccctg aactctacgc tgtagctcaa acaagtggtg agattccaga
      421 aaaaaaaacg cgaagaattg cgtcagatta tcacaactat ctttgaacaa aagatgaata
      481 agcatagtat taagtagaga atgaaaaaat attttattgg cggtttggga agcaatgcct
      541 atcatagcaa ggattttctt caagaactag attcgcaggt ctattttcta aatccatatg
      601 aaaagcatct tcgagatgaa acagaattga aatcatggtt taaaaatgag attgtagagg
      661 aagaatctat ctgtctgata ggtcattcta ttggaggcga tttagctcgt tatttcgcat
      721 cggaatttga agaagtaaag aaactgattc ttttggatgg tggctatcta gatttagata
      781 agattttacc tttggataca gagttagagg aaactaaaaa ttatatcaaa tctcaaatta
      841 tttcggactt agatgttctt acttctaaag aaaaatctga agcaaagcat tggtcagaaa
      901 atatggagaa agctgtaaga cagtcctatc actggaatgt tgagtataat agatatgagt
      961 tggctataaa ttatgaaaat atagaagcga tactccgcct acggagaaaa atacaagctt
     1021 ttaagagaga agtgggagat accttgttta ttagtcctcg ctatcctaat gaagctacat
     1081 ggagagagga agccctaaaa gaattgccag actattttga tactattttt ttagaaaact
     1141 ttggccatga gctttatact caagcaccta aagaaatcgc tagtctaat
//
LOCUS       DAVAAL010000218         1110 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVAAL010000218 DAVAAL010000000
VERSION     DAVAAL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.221"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..916
                     /locus_tag="VQM60_002041"
     CDS             <1..916
                     /locus_tag="VQM60_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET1081850.1"
                     /translation="TVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDA
                     ILKQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPS
                     PVPYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVS
                     FKEVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKV
                     AQNLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTL
                     KNSENEAVLEEVRSAVKELTDAFPLYED"
     gene            924..>1110
                     /locus_tag="VQM60_002042"
     CDS             924..>1110
                     /locus_tag="VQM60_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081851.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFN"
BASE COUNT          339 a          223 c          233 g          315 t
ORIGIN      
        1 tacggttatg ggaatggatt tggcatcagg tggtcatttg actcatgggg ctcctgttag
       61 cttctctggt caaacctaca actttgtttc ttatagtgtt gatcctgaaa cggaactctt
      121 agactttgat gctatcttga aacaagccca agaagtaaaa ccaaaactga ttgtagctgg
      181 tgcttcagca tattctcaaa ttatcgattt ttcaaaattc cgtgaaatcg cagatgctgt
      241 cggtgcgaag ctcatggtgg acatggccca tatcgctggt ttggttgcgg ctggccttca
      301 tccaagccca gttccatacg ctcatatcac aacaacaacg acccacaaaa cccttcgtgg
      361 acctcgtggt ggtttgattt tgaccaatga cgaagaactt gctaaaaaaa tcaattcagc
      421 tattttccca ggtattcagg gcggtccttt agagcatgtt gtggcggcta aggcagtttc
      481 cttcaaagaa gttttggatc cagccttcaa ggaatatgct gccaatgtaa ttaagaacag
      541 caaggctatg gcagatgtct tcttgcaaga ccctgatttc cgtattattt caggtggaac
      601 tgaaaaccat ctcttccttg ttgatgtgac taaggttgta gaaaacggaa aagttgctca
      661 aaacttgctg gatgaagtca atattaccct aaataaaaat tcaatccctt acgaaacctt
      721 gtcgccattc aagacaagtg ggattcgtat cggagcagca gctattactg cacgtggatt
      781 tggtgaagaa gaaagtcgca aagtggctga actcatcatt aaaaccctta agaattcaga
      841 aaatgaggct gtattagaag aagtgagaag tgcagtcaaa gaattgacag atgccttccc
      901 attatacgag gactaaaact tttatggata tttatattaa gaaagccatt attcaccagt
      961 tcagtccgga tgataccgag ctgttcttag cagataagtt tctcaatatt actccaaaaa
     1021 tcgaagaata cctacgtaaa aaaattgaac atgtgtattc agatgaagcc aagactggga
     1081 ttttcgaaga agaaaatccc ttcttcaatc
//
LOCUS       DAVAAL010000219         1106 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVAAL010000219 DAVAAL010000000
VERSION     DAVAAL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.222"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(281..>1106)
                     /gene="pta"
                     /locus_tag="VQM60_002043"
     CDS             complement(281..>1106)
                     /gene="pta"
                     /locus_tag="VQM60_002043"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET1081852.1"
                     /translation="KIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEE
                     DVRKVLVEDVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAF
                     LMVRGTERYLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFG
                     ESVDKVVEATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGI
                     EAGNIGYKMAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT          328 a          244 c          207 g          327 t
ORIGIN      
        1 gaaaacgcat aatagcaggt gtcaagacac ttaatattat gcgttttctc atgaaaaaat
       61 tctcttttca tactcattta aaatcaaaaa gggaaattat cgttttacgg acatacatat
      121 tacagatttc tataggtcgt gtatagacag gatatcatcg cttggaagtt ttcctttttg
      181 acaccaatca gtaatttagt tctcaggaat taatggcagt gcttttcgta catagtttca
      241 taactacagt atagcatagt atatcgcttt atagttttca ctattgatga actgcttgag
      301 ctgctgtgat gagggtcaac ttgtaaacat catctgcatt acatccacga gaaagatcat
      361 taactggctt gtttaaacct tgcaaaacag gtcctacagc cgcaaagcca cccaggcgtt
      421 cagccatctt gtaaccgata tttcctgcct cgatacctgg gaagatgaag acatttgctt
      481 gaccagctac cgtacttcca ggagctttca gagctgcagt ttcaggaaca aaggctgcat
      541 caaattgcaa ctcaccatcg atttcaaggt caggacgcaa gtcgtgagca attttagttg
      601 cttcaacgac cttatcaacg ctttcaccaa accctgaacc tttagtagaa tagctcaaca
      661 tggcaatttt aggttcgatg ccaaacatct tagctgtgat tgctgagttg atggcaattt
      721 cagccaaggc ttctgcatct ggattgatgt taatggcaca gtctccaaat aggtaacgtt
      781 ccgtaccacg aaccatgagg aaggctcctg aagtacgagt tacatttgga cgagttttga
      841 tgatttgtag agctggacga actgttgaag ctgttgagtg aatcgctcct gacaccattc
      901 catcaaccaa gcccaagtaa accaacatca caccaaagta gttgacatct tcaaccaaaa
      961 ccttgcgtac atcttcttca gtcattttgc ccttgcgacg ctccaccaag gcagaaacca
     1021 tttcttcaaa ttgaggataa tgttgagggt cgatgacctc ataaccatcc atgatccctt
     1081 caatttcaag ataaatttta attttt
//
LOCUS       DAVAAL010000220         1099 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVAAL010000220 DAVAAL010000000
VERSION     DAVAAL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.223"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(488..>1099)
                     /locus_tag="VQM60_002044"
     CDS             complement(488..>1099)
                     /locus_tag="VQM60_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET1081853.1"
                     /translation="MPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYT
                     LEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNGQADTNQTEKPNEEKPQTEKP
                     EEETPREEKPQSEKPESPKPTEEPEEESPEESEEPQVETEKVEEKLREAEDLLGKIQD
                     PIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          294 a          220 c          187 g          398 t
ORIGIN      
        1 aacaacacct tccgcaatgt gtttggaatg tgaaacgata acaagaccaa taccaatact
       61 ttccatcaaa ccaccccagt ttctaaaata gcagtaaaga gtaatcctga tgagaatgat
      121 ccaggatcaa tatgtccaag aaaccacatg ctcctaagac aagagctaac agactggcca
      181 tcaataatag tattgttctt tttttcatca ttactcctta actagtgttt aactgattaa
      241 ttagccagta aatagtttat ctttatttac actatctgtt aagatatagt aaaatgaaat
      301 aagaacagga cagtcaaatc gatttctaac aatgttttag aagtagaggt atactattct
      361 agtttcaatc tactatattt tgcacatttt catgaaaaaa atgagaacta gaactcacat
      421 tctgctctca tttttcgttt tcccgttctc ctatcctgtt tttaggagtt agaaaatgct
      481 gctaccttta cttactctcc tttaataaag ccaatagttt ttcagcttct gccataatag
      541 tattgttgtc ctgggtgcca aatagtaaat tattttttaa tcctgtgaga gtctctttgg
      601 cattggactt gataattgga tcctggattt ttccaagtaa atcttcagcc tctctcagtt
      661 tttcttcaac cttttcagtc tcgacctgag gttcttctga ttcctctggt gattcttctt
      721 ctggttcctc tgttggtttt ggagactctg gtttctcgct ttgcggtttc tcttctcgag
      781 gggtttcttc ctcaggtttt tctgtctgag gtttctcctc gtttggtttc tccgtttgat
      841 tggtatcagc ttgaccattt ttgtttcttt gaacatggtc gctagcgtta ccaaaaccat
      901 tatctgaatg cggacgttcg tttggatgtt cgacatagta cttgacagtc gccaaaagat
      961 cctcaagagt atacccctta ggtgcctcat aaaggccttc gtcaaaccac tcaaatttga
     1021 tgttatggta atggtcataa tgaggtatga ttaaactacc gtttttgact tctacagtat
     1081 attgaagatt gtaaggcat
//
LOCUS       DAVAAL010000221         1055 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVAAL010000221 DAVAAL010000000
VERSION     DAVAAL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.224"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..393)
                     /locus_tag="VQM60_002045"
     CDS             complement(<1..393)
                     /locus_tag="VQM60_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET1081854.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLI"
     gene            complement(575..>1055)
                     /locus_tag="VQM60_002046"
     CDS             complement(575..>1055)
                     /locus_tag="VQM60_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HET1081855.1"
                     /translation="YSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLG
                     FNELPEEEQEKLKEWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMN
                     MGQDPLFPDSADDVNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEVMQDDDYNYGLD"
BASE COUNT          300 a          195 c          202 g          358 t
ORIGIN      
        1 aatcagaaaa acaggtagac tgttggttac gacgcggata ttgtcaatag gcaactcacg
       61 cgcaaaaaac tctaatgttg ttcctggtcc aatgaaaata gtttctcttt cttctactag
      121 actgcctgca aaatgggcta tttcttgttt ttctgccgtt tggagggctt gtttttcaat
      181 atttgatcgc tcattagtca aaagggagtt ggttcgaagt ttttcagctc caccatgcac
      241 acgaatcagc aaatctttat cagctaattc ctgtaaatag cgccttgcag tcatatctga
      301 aacggctatt tcgtccataa tctgtttaac cgttatggtt cctttgctat ttactatctc
      361 taaaattttg gttaattttt cttgtttgag catattatca cctcgtttcc tactactatc
      421 ttaccataaa caaactcatc attcaaatac aaaaacaaca aaatgaaaca aaaacaaaaa
      481 tatcgaagtt tgttttcaaa actttcgata tttttgttgg gttataactt tgatgtttct
      541 agtttacttt ttgatgattg agagtggtgg agaattagtc taaaccgtag ttatagtcat
      601 cgtcttgcat gacttcaact tcgccaagaa ggtaaccatt tccgacttga gagaagaagt
      661 cgtggttgga agttcctgtt gaaataccgt tcattacgat tgggttgaca tcatcagctg
      721 aatctgggaa gagtggatct tgtcccatgt tcatgagagc tttattggca ttgtagcgaa
      781 ggaaggtttt aacctcttca gtccaaccaa caccgtcata aagactctct gtgtagcctt
      841 cttcattttc ataaagagta tagagtaggt cgtacatcca ttctttgagt ttttcttgct
      901 cttcttcagg taattcattg aaaccaagtt ggaatttgta accaatgtag gttccgtgaa
      961 cagactcgtc acgaataatc aatttaatga tttctgcaac gttggcaagt ttgttgttac
     1021 cgagatagta gaggggagtg aagaaaccag agtag
//
LOCUS       DAVAAL010000222         1038 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVAAL010000222 DAVAAL010000000
VERSION     DAVAAL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.225"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            107..>1038
                     /gene="pspC"
                     /locus_tag="VQM60_002047"
     CDS             107..>1038
                     /gene="pspC"
                     /locus_tag="VQM60_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HET1081856.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATEKEGST
                     QAATSFNRGNGSQAEQRGELDLERDKAMKAVSEYVGKMVRDAYVKSDRKRHKNTVALV
                     NQLGNIKNRYLNEIVHSTSKSQLQELMMKSQSEVDEAVSKFEKDSFSSSSSGSSTKPE
                     TPQPENPEHQKPTTPAPDTKPSPQPEGKKPSVPDINQEKEKAKLAVATYMSKILDDIQ
                     KHHLQKEKHRQIVALIKELDELKKQALSEIDNVNTKVEIENTVHKIFADMDAVVTKFK
                     KGLTQDTPKEPGNKKPSAPKPGMQPSPQPEVKPQL"
BASE COUNT          407 a          189 c          203 g          239 t
ORIGIN      
        1 tttacccact acaaatatta tagagccaaa taatgtagca aatttcatat acaattcata
       61 ttagcaggat atactgtagt taattaaaat tatggagaat atagacatgt ttaaatcaaa
      121 tcatgagcga agaatgcgtt attccattcg taaatttagt gtaggagtag ctagcgtagc
      181 tgttgccagt ctttttatgg gaagtgttgt acatgcgaca gagaaagagg gaagtaccca
      241 agcagccact tcttttaata ggggaaatgg aagtcaggca gaacaacgtg gagaactcga
      301 tttagaacga gataaggcaa tgaaagcggt cagtgaatat gtaggaaaaa tggtgagaga
      361 tgcctatgta aaatcagata gaaaacgaca taaaaatact gtagctctag ttaaccagtt
      421 gggaaacatt aagaacaggt atttgaatga aatagttcat tcaacctcaa aaagccaact
      481 acaggaactg atgatgaaga gtcaatcaga agtagatgaa gctgtgtcta aatttgaaaa
      541 ggactcattt tcttcgtcaa gttcaggatc ctccactaaa ccagaaactc cgcagccgga
      601 aaatccagag catcaaaaac caacaactcc agctccggat accaaaccaa gccctcaacc
      661 agaaggcaag aaaccaagcg taccagacat taatcaggaa aaagaaaaag ctaagcttgc
      721 tgtagcaacc tacatgagca agattttaga tgatatacaa aaacatcatc tgcagaaaga
      781 aaaacatcgt cagattgttg ctcttattaa ggagcttgat gagcttaaaa agcaagctct
      841 ttctgaaatt gataatgtaa ataccaaagt agaaattgaa aatacagtcc acaagatatt
      901 tgcagacatg gatgcagttg tgactaaatt caaaaaaggc ttaactcagg acacaccaaa
      961 agaaccaggt aacaaaaaac catctgctcc aaaaccaggt atgcaaccaa gtcctcaacc
     1021 agaggttaaa ccgcagct
//
LOCUS       DAVAAL010000223         1037 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVAAL010000223 DAVAAL010000000
VERSION     DAVAAL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.226"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            33..484
                     /locus_tag="VQM60_002048"
                     /pseudo
     CDS             33..484
                     /locus_tag="VQM60_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            649..948
                     /locus_tag="VQM60_002049"
     CDS             649..948
                     /locus_tag="VQM60_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET1081857.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
BASE COUNT          367 a          206 c          180 g          284 t
ORIGIN      
        1 taataacgat actagataat gaggaaaatg caatggcaaa cccaactttc ggagaaaaaa
       61 agtgaataca gactatattg ctcgctatgg cgtatatgca gttatcccta atcctgaaca
      121 aaaacaaatt gttcttgttc aagaaccaaa tggtgcttgg ttcctaccat gtggaaaaat
      181 tgaagcaggt gaaaatcatc aggaagccct aaagcgtgag ttgattgaag agcttggttt
      241 cacagcagaa attggtacct attacggaca agctgacgaa tatttctatt ctcgtcatcg
      301 tgatacctac tactacaatc ctgcctacct ctatgaagca actcctttca aagaagtaca
      361 aaagccacta gaaaacttta atcatattgc ctggttccct attgacgagg ctatcaaaaa
      421 ccttaaacgt ggtagccata aatgggccat tgaatcttgg aaaaaacagc ataagattgg
      481 ctaatactct tcgaaaataa aattcaaatc acgtaagcgt cgccttaccg tactcaagta
      541 tagcctgcgg ctagcttcct agtttgctct tttattttca ttgagtataa atcaagcgac
      601 tttattcaaa aagtgctata atagaattag aaaatcggag gaaatattat gaagcttatc
      661 aatacgacaa actcacactc gcaacttgta aaaagtcagc tagaaagtac cgatgcaacc
      721 cttgttgagg tttattctgc agggaataca gatgttattt ttacccaagc tccgcttcac
      781 tatgaaatcc tcatctcaaa caaacaccgt gctattcgtg agcctgaaat cgaaaccatt
      841 caagaatttt tcttgaaacg taaaattgat aaggcctctg ttgatgaagc caatatcaag
      901 acactctact cagagaaatt aattggaatt tcaatcccaa tcaagtaaga aaattgagta
      961 aaaactcgat ttgagagaaa gtccaataag tctttcaata ataaaacgca tagtagcaag
     1021 agttttgtac tgacccc
//
LOCUS       DAVAAL010000224          891 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVAAL010000224 DAVAAL010000000
VERSION     DAVAAL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.227"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            44..841
                     /locus_tag="VQM60_002050"
                     /pseudo
     CDS             44..841
                     /locus_tag="VQM60_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          296 a          185 c          141 g          269 t
ORIGIN      
        1 tataggtatt atggttctga ttttctagta taaaaaaatt caaactagaa ttcttcttag
       61 aaaaggccgt tttaagtgct atcattgctc aaaaatggag gttgctgaga cttctatcgt
      121 caagaagaat catcaaattc cttgtatcat caaccaaaag attgctcaga agctaattga
      181 aaagacttct atgaccgaca ttgatcatca gttgtctatt tcaacttcaa ctgtcattcg
      241 caagatcaat gattttcact ttgagcatga tttttcgcgt cttcctgaga ttatgtccta
      301 ggacgttgaa acagtctggg gagtgactgt ttcaatcggg agatagagat gagctttatt
      361 gctcaggact ttgataagct caatatcata actgttcttg agagcagaac acaagccatc
      421 atccgaaatc ccatgaatac aaggctatca agcgatactg aaagctcatt caacaagata
      481 gtcagaaact gagtgataaa cgattttatc gccctacttt tcgtatgcac ttgactaata
      541 aagaaattct agataaactt ttgagctatt ccgaagactt gaaacaccac tataatctct
      601 atcagctctt gcttttccac ctctttttca gactctgttt aaaaccttcc tcaaagataa
      661 agagaaaatc gtcaacgccc ttcaattacc ttattccaac gccaaactgg aagccaccaa
      721 taatctcatc aaacttatca agcacaatgc ctttggtttt aggaactttg aaaacttcaa
      781 aaaagaaagg acgaaatttg tcctttctag atcttcgctt tcttcaaccc actacagttg
      841 acaaagaacc gaaaaatgtc tcgcctctaa tatagtcaat tgaaacaaga a
//
LOCUS       DAVAAL010000225          880 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVAAL010000225 DAVAAL010000000
VERSION     DAVAAL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.228"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
BASE COUNT          292 a          143 c          188 g          257 t
ORIGIN      
        1 taaaataggc tccataatat ctatagggga tttacccact acaaatatta tagaaccgga
       61 ttttgtggta cgacaggcat gtatagcaaa ctgaatctgg aatagcacag catatcttct
      121 aaaatattgt tataaattga tttgaatttc aaaattaaat tgtttgattc ttatttcaat
      181 ttgttatagt atatctgatg tcaaagttct cggcgagtca aatagcgatt cccaagcctg
      241 actatcgtga ggtagcggat taaaatggtc tggggataga ccgttttaag tctgacgctg
      301 gaaataagaa ttgtcagaag aagggatagc gaaatcgtgg ctctacgaac aggaacgtga
      361 taataaggcg tatatagcgg ataagggggc ctcaaactct aaagtccaaa aaggtagtcg
      421 taacctatat gcgtaaatca cgagagtaat tgaattcgta ctaagatttt ctattttcac
      481 tgtaaccttt taacgtcctt atatcttgta tacacgagga aagatgtacg acttatcccg
      541 tgaggtctat cactataaag agaaaacgac agatagaagc gatcctgagt cacggttatc
      601 tttaaaatag attgtctgat aggatgctag cctgaaaacg tcttgttcat gaaatatagg
      661 gacggtatgt ataaaacgct tctgtgaact gagagaaggg ggagaagttc ttgataaaat
      721 ttagttgaac agccgtattc cgatacttag ataagagatc tagtcttagc tcctactcgg
      781 tttcagagga taaaaaaggg caatagcgat tcgagaaaga ttatactctt cgaaaatctc
      841 ttcaaatcac gtcaatatcg ccttgccgta cgtgtaggat
//
LOCUS       DAVAAL010000226          858 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVAAL010000226 DAVAAL010000000
VERSION     DAVAAL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.229"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            105..584
                     /locus_tag="VQM60_002051"
     CDS             105..584
                     /locus_tag="VQM60_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET1081858.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            678..749
                     /locus_tag="VQM60_002052"
     tRNA            678..749
                     /locus_tag="VQM60_002052"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:711..713,aa:Glu,seq:ttc)
BASE COUNT          317 a          117 c          199 g          225 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtttataagt gtagaaatga atattacctt catttatggg
      181 aattgtcgga ttgggaccaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caaaaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aagcaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggagagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ctagtaagtc atgcaaaaaa
      601 aatgaaaaaa attagaaaaa gtagttgaca aagtttgaaa agactgtata atagtaagag
      661 ttgaaaataa caactcaggt ccgttggtca aggggttaag acaccgcctt ttcacggcgg
      721 taacacgggt tcgaatcccg tacggactat ggtatgttgc ggttggaaca cttgatgaaa
      781 aaaagtttaa aaaagcttaa aaatcttcaa aaaagtgttg acaagcgaaa gcggctgtga
      841 tatactaata tagttgtc
//
LOCUS       DAVAAL010000227          848 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVAAL010000227 DAVAAL010000000
VERSION     DAVAAL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.230"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            275..526
                     /locus_tag="VQM60_002053"
     CDS             275..526
                     /locus_tag="VQM60_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET1081859.1"
                     /translation="MKEFQFERKQRFSLRKYVIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEY"
     gene            514..729
                     /locus_tag="VQM60_002054"
     CDS             514..729
                     /locus_tag="VQM60_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081860.1"
                     /translation="MGVLGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGK"
BASE COUNT          321 a          107 c          165 g          255 t
ORIGIN      
        1 ttatctacta cctcaaaacg gtgttttgag caacctgtgg ctagtttcct agtttgctct
       61 ttgattttca ttgagtataa agagcttggc aacaggctct ttttgtattg cgtttattaa
      121 gaaaaacaaa aaacatcgat atacatttaa ctaaaaatta ttgtatgtta tctcccttaa
      181 ttaggaatga taagggaata actagaaaga tttgtgaata caaactattt ctgatataca
      241 aaatatacag taataatgaa tgatgggaga tgggatgaaa gaatttcaat ttgagagaaa
      301 gcagcgtttt tctttgagga aatatgtaat aggagcttgt tcggtcttgc taggaacgag
      361 tttatttttt gctggtatgg gtgctcagcc tgtacaggat acagaaacga gttcagcact
      421 aatttcaagt cattatttgg atgagcagga tttatctgaa aagctgaaat ctgagttgca
      481 atggtttgaa ttagaaaaca agcttttgaa cttatgggag tattaggtta ctatgaagga
      541 tttgttcctt atgtttcaaa tcaatacaaa aaccaagctg aagaagaagg caaaccgcta
      601 tctgataaat atattttcga aaaaatctta ggaaaaacat atgcagcctt caaaaaagat
      661 caaattaatg agcgtgtcga gaaattaggt aagttaaaac ctattacaat aaattacaac
      721 ggaaaataag aagtaattga tagtaaagaa aaattacaag agcttatgaa taaagccgtt
      781 aaagacgaag tggctcaaat atagtaaaat gaaataagaa caggataaat cgatcaggac
      841 agtcaaat
//
LOCUS       DAVAAL010000228          846 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVAAL010000228 DAVAAL010000000
VERSION     DAVAAL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.231"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            95..435
                     /gene="tnpB"
                     /locus_tag="VQM60_002055"
                     /pseudo
     CDS             95..435
                     /gene="tnpB"
                     /locus_tag="VQM60_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(468..846)
                     /locus_tag="VQM60_002056"
                     /pseudo
     CDS             complement(468..846)
                     /locus_tag="VQM60_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          258 a          166 c          154 g          268 t
ORIGIN      
        1 ttacgtaagc gcgtcataac aaggtatcta tcctaattcc ccaacttcca gtatactaga
       61 taaaaaataa ttatcggagg aaaggtattg tactatgata attcaactaa gtgatttagg
      121 tcaagttcac cttgtttgta gcaagataga tatgaggcag ggaatagact cattagccta
      181 tgtagttaaa acccactttg aattggatcc tttctccggt caagtctttc tcttttgtgg
      241 tggacgtaaa gaccgcttta aagcccttta ctgggatggt caaggatttt ggctactata
      301 taaacgcttt gagaacggca gactgacttg gcccagtaca gaaaaggatg tcaaagctct
      361 cacacctgaa caagtagact ggcttatgaa gggcttttct atcactccaa aaataaatcc
      421 atcagaaagt cgtgatttct attgaaatga ggactttctt tttatactca tctgctttca
      481 aaaagcattc tagtccatct ccgattaacg atgggcttta tcacctcctt ctccagtcct
      541 tgtatgatat cttaaagttg attcatgaca tcttccaaag tttgaaaggc tttattttta
      601 aatccacgtt tacgaatctc tttccacact tgttcaatgg ggttcatctc tggtgtgtac
      661 ggaggaataa atgcaaagcc aatattagtc ggaatcttta aggtacttga tttatgccat
      721 atagcattgt ccataacgaa taaaagataa tcatctggat aagcttgtga aagctcttct
      781 aaaaagacgt tcatccactc agtattacat ccaccagcta ttaagaaaaa tgattcgcct
      841 gtatgg
//
LOCUS       DAVAAL010000229          814 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVAAL010000229 DAVAAL010000000
VERSION     DAVAAL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.232"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            86..808
                     /locus_tag="VQM60_002057"
     CDS             86..808
                     /locus_tag="VQM60_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET1081861.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT          242 a          136 c          214 g          222 t
ORIGIN      
        1 ggtttgaaga gatttttgaa gagtatgaaa cgaaaaggag gttggtcatt gtactagcct
       61 ccttttgata tgatataata gttctatgag attagataaa tttttagttg cctgtgcggt
      121 gggaagtcgg actgaggtca aaaacttgct caaggctggg cgcgtgactg taaatggtaa
      181 aaaagaaaaa tcagctaaat tgcagattga tgaaaaaata gatgagattc gctttgatgg
      241 gcaagtgttg gagtatgaag agtttgtcta ctacatgatg aacaagccca aaggagttat
      301 ctcagcgact gaggatccca agcacagaac cgttctggac ttgttggatg acttggcgcg
      361 gagtaaggaa gttttcccag taggacgctt ggatattgac acgcatggtc ttttgctctt
      421 gaccaatgat ggtcagctgg ctcatgttct tctttcgccc aagcgtcatg tggacaagac
      481 ttatctggca caagtcaagg gaatcatgac ccaagaagat gtggagacat ttgctgaggg
      541 tattcctctc aaagacttta cctgtcaacc cgctatactg gagcttgtat ccatagatac
      601 agaaaagaat caaagccaaa tccgtgtgac cattgcagaa gggaagtttc atcagatcaa
      661 gcgtatggtg ggctattgtg gcaaggaagt ggtggacttg caacgattga ctatgggaac
      721 tttagtatta gatgagaacc tggaacgagg ggaatggcgt cgcttgacca aggaagaatt
      781 agaaattctc cgcgctaata ttatttaatt tagt
//
LOCUS       DAVAAL010000230          804 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVAAL010000230 DAVAAL010000000
VERSION     DAVAAL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.233"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            149..682
                     /gene="queF"
                     /locus_tag="VQM60_002058"
     CDS             149..682
                     /gene="queF"
                     /locus_tag="VQM60_002058"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET1081862.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQHHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT          237 a          187 c          159 g          221 t
ORIGIN      
        1 tcctgttctt atttcatttt actatataag tagaatatta cgatgtttgg ggtatgccat
       61 cgctaaaaaa actcgatttg gtatttttta gttccctaat gggagctttt tgcgtgctct
      121 gaacatttcc cattttggaa ggagtactgt gaaacgtcaa tcagccttgg tcgtctttag
      181 tggtggtcaa gattcaacaa cctgcctttt ctgggccatg caacactatg aaacagtcga
      241 agctgtcacc tttgcctacg gccaacatca tcacctcgaa attcaaattg ctagagaaat
      301 cgccaaggaa cagggcattc gtcaccatat cctcgatatg tctctgctgg gacaaatcac
      361 tgctcaacca gactttgcga cgattcatat ttcctacatt cctgacaagc tctgtgtcga
      421 gtcaaaatcc ctcaaactat atctatttag ctaccgaaac cacggagatt tccacgaaaa
      481 ctgtatcaac accatcggga aagacttggt caacttgcta gaccctcgct atttagaagt
      541 ctggggaaaa ttcactccgc gcggtggcat ttcaatcgac ccctactata actacggtaa
      601 gcaaggaact aagtatgagg gcttggcaga acaacgcctc ttccaacacg acctttatcc
      661 agagaaaatt gacaaccgct aaactcatac tcaatgaaaa tcaaagagca aactaggaag
      721 ttagccgcag gttgctcaaa gcagtgtttg aggttataga taagactgac gaagtcagct
      781 taaaacactg ttttgaggtt gcag
//
LOCUS       DAVAAL010000231          770 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVAAL010000231 DAVAAL010000000
VERSION     DAVAAL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.234"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            162..698
                     /locus_tag="VQM60_002059"
     CDS             162..698
                     /locus_tag="VQM60_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET1081863.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
BASE COUNT          186 a          163 c          141 g          280 t
ORIGIN      
        1 tcttttttaa tgattttcga atcaacaaaa aaattatcca acttgactag tcacgaaaat
       61 cggctgttac agtttttgtt accttgtttt taaaaaatgg ttgacatcga ctcctatatg
      121 agtataatag ttactataca ttagaaaaga ggttaactat tttgaaaaaa gctcacgttt
      181 atgctatccc tgctattggg gctgctctca ttgctgcttt ggcacaaatc agtcttccaa
      241 ttggacctgt tcccttcact ctgcaaaact ttgcaatcgg cttgattgct actgtcttta
      301 gaccgagaga ggctgtactt tctgctggac tctatcttct tctaggtgct atcggtcttc
      361 ctgtctttgc aggaggtgga gctggttttc aggctttagt tggccctact gcaggctatc
      421 tttggtttta tctcgtttac tctggactta cttcctctct aaccaacagc aagagtggtg
      481 ttgttaagat ttttcttgca aacctcttgg gtgatgccct tgtctttgtc ggcgggattc
      541 tcagcttgca tttcctagct ggaatggcat ttgaaaaagc tcttgctgtg ggggttcttc
      601 cctttatcat tccagacctt ggcaaacttc tggctattag ttttattagc cgtcccctac
      661 ttcaacgcct taaaaatcag gcttacttta ctaactaaaa aaggatatcg agttatcatg
      721 actcaatatc cttttctttt attttgaaaa cttatactca atgaaaatca
//
LOCUS       DAVAAL010000232          756 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVAAL010000232 DAVAAL010000000
VERSION     DAVAAL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.235"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..756)
                     /locus_tag="VQM60_002060"
     CDS             complement(<1..756)
                     /locus_tag="VQM60_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET1081864.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYTEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNL"
BASE COUNT          250 a          125 c           91 g          290 t
ORIGIN      
        1 taaatttttt aatttagtac cactcatttt agatattttt tcaagcgtgc cttgatttac
       61 attcgttgca aaatgatcat ctaagaatct tgctacatct tcaagtgctt tatcatcatc
      121 aatttcaatt ttatattttt ttctatttaa gtatgtgtca attactatac ttatccattc
      181 atttgccttt gctttaaaga aaaaatcagc ggcaggagcg tccatcttac aatttaatat
      241 ttccattgcc actctttcta aggcctttgt aagtattatt tgattcggtt gaagcaaggt
      301 tgaataaaaa gattctggat taatgttaat agatgctaaa tgtttttcta ttagctcttt
      361 tttaaaacca atggaaacag caagataaca acaattctcg tgtaataaaa aaacaaaatc
      421 atcttttata ttatcaaaat caaaagtaca taaagagttt gcggtaatag tttgatacgg
      481 attaaacttt tctccgtttg cactgacaat gtaacttgaa taaattgaaa catagtctga
      541 catactataa gtgctatttt gaactacttc ctctttgata taaaaatcat gtatatcgat
      601 aatgaagctg cctccttcat aaaaccaata taggccttct gtataagttg aattctcttt
      661 tttccaacaa aatgtatgac ctgcacttga atacttctca ttgtttttac attcatctac
      721 tttcatataa tcttttacaa aatcataata tgacat
//
LOCUS       DAVAAL010000233          727 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVAAL010000233 DAVAAL010000000
VERSION     DAVAAL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.236"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..243)
                     /locus_tag="VQM60_002061"
     CDS             complement(<1..243)
                     /locus_tag="VQM60_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HET1081865.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLG"
     gene            complement(543..725)
                     /locus_tag="VQM60_002062"
     CDS             complement(543..725)
                     /locus_tag="VQM60_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET1081866.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIASELVAGLR"
BASE COUNT          241 a          129 c           89 g          268 t
ORIGIN      
        1 acctaggtta attatttttt cggtgatgta caccaactcc aaaggaacgt taataaacat
       61 ctctgtaaaa cgggtcatat tatctctatt tttgagaaaa aaacttttct caatatcaat
      121 tggatttact ttatctccag tttttcttcc aaaagctatt ccccttccaa aaagaagaat
      181 atcgatatct tttttacttt gcgcaattac ggcattgtgg tttagtattc gttttatgat
      241 cataaaattt cccttttaaa ataaaaggaa cctacttaaa aacatcaaga aacttcttcc
      301 gatgtgatta aataggttcc tgctcacttg tattagtgat aacatccttt tatttcaatt
      361 tttaatggtg acatgaagct taaatcatta ataactctgt aaccattttc attttaagat
      421 atttcaatct atttgtcaag tacttataga aattttttta ttttgtgtga ttcgaatgtg
      481 ataatgacaa cagtctcact aaagaaatga aaatcataca tagaaatttt taaaaatttt
      541 ctctatcgta aacctgccac tagttcagaa gctatactag ctgattccaa acgtaatatg
      601 gtcatctcat ctaccttccc taataatggc attgcaccat accaaacaat agtcttattc
      661 accaaaagaa taggcgttac tatttttgat tgagtaaaag acactttaat tttctggctc
      721 ttcaact
//
LOCUS       DAVAAL010000234          708 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVAAL010000234 DAVAAL010000000
VERSION     DAVAAL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.237"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..613
                     /locus_tag="VQM60_002063"
     CDS             <1..613
                     /locus_tag="VQM60_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET1081867.1"
                     /translation="LKEGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRG
                     KSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLG
                     IKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKTSPFIFFDEPSSG
                     MDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          281 a           80 c          126 g          221 t
ORIGIN      
        1 tttaaaagaa ggtggagagt atcagataaa aaatcttagc tacaaattta ctgatgatga
       61 gtgtttaagc ttaaaagata tttcattcaa gcttgggaaa atttatggca taataggatc
      121 caacggacga ggaaaatcaa cgcttttaag atgtttaata ggtcttgaga aaaaatcaaa
      181 agaagaaatt tattttaagg gagagaagct atctaaaaaa gaaagactca aaaactcttc
      241 acttgttatg caagatgtaa atcatcaatt attcacagat gaggtattca acgagcttag
      301 attaggaata aagaattttg atgaagaaaa ggcgaaaatc attttaaaag atttaggcct
      361 ggacgaattt attgaaaggc atccgatgag tttatcagga gggcaaaagc aaaggcttgc
      421 aatagcatct gttatgtgca agacttctcc atttatcttt tttgacgaac cttcaagtgg
      481 tatggattat tccaatatga taaaaatatc tgaactgatt aataagtata aaaccatgga
      541 taaaataatt tttattgttt cccatgatat agaattttta aatgaagtgg cagatgaaat
      601 ttttgaatta taaaatagag tctgttaaac atgtctatag aactggtaaa ttgtctaaaa
      661 atttaaaaga tactaacaca tattgatatt tattagaaaa ttaacttc
//
LOCUS       DAVAAL010000235          706 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVAAL010000235 DAVAAL010000000
VERSION     DAVAAL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.238"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            79..681
                     /locus_tag="VQM60_002064"
                     /pseudo
     CDS             79..681
                     /locus_tag="VQM60_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT          258 a          121 c          125 g          202 t
ORIGIN      
        1 gttatagatt caatatacta tagaatagaa ttgtaggttt taaaataaac ttaaataaca
       61 caacggagat atcccatcat gaaaaagaaa tggatgtatt atgctgcttg ttcttctaat
      121 gaatctgccg atgacagttc atctgataaa ggagacggtg gttcgctagt cgtttattga
      181 ccaaactcag aggacttaat tggagcaact attcctgcct ttgaagaaaa atatggtatc
      241 aaagtagaac tgattcaagc tggtactgga gaacttttca aaaaactaga gtcagaaaaa
      301 gaagttcctg tagctgatgt tatctttggt ggttcttata cacaatatgc tacccacgga
      361 gaactctttg aaaactatac ttcaaaagaa aatgataatg ttatcaaaga atatcaaaac
      421 acaactggct actctactcc ttatacacta gatggtagtg ttttaatcgt caaccctgat
      481 ttaactaaag gcatgaacat cgaaggatat aacgatcttc tcaaacctga actaaaagga
      541 aaaatcgcaa ctgccgaccc agcaaactct tctagcgcct ttgctcaatt aacaaatatg
      601 ctacaagctc aaggtggtta caaagatgat aaggcttggt cttatgtaaa agatcttttc
      661 acacttattg atggtatagt aaaatgaaat aagaacagga caaatt
//
LOCUS       DAVAAL010000236          700 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVAAL010000236 DAVAAL010000000
VERSION     DAVAAL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.239"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..>700
                     /locus_tag="VQM60_002065"
     CDS             <1..>700
                     /locus_tag="VQM60_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET1081868.1"
                     /translation="GLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKSVNPDEVV
                     AMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSKSQVFSTA
                     ADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDKNGIVSVK
                     AKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNEVDQAIFA
                     TEKTIKETEGKGFDA"
BASE COUNT          229 a          152 c          154 g          165 t
ORIGIN      
        1 caggtttgag cttgtcagaa atcgacgaag ttatccttgt tggtggttca actcgtatcc
       61 ctgccgttgt tgaagctgtt aaagctgaaa ctggtaaaga accaaacaaa tcagtaaacc
      121 ctgatgaagt agttgctatg ggtgcggcta tccaaggtgg tgtgattact ggtgatgtca
      181 aggacgttgt ccttcttgat gtaacgccat tgtcacttgg tatcgaaaca atgggtggag
      241 tatttacaaa acttatcgat cgcaacacta caatcccaac atctaaatca caagtcttct
      301 caacagcagc agacaaccaa ccagccgttg atatccacgt tcttcaaggt gaacgcccaa
      361 tggcagcaga taacaagact cttggacgct tccaattgac tgatatccca gctgcacctc
      421 gtggaattcc tcaaatcgaa gtaacatttg acatcgacaa gaacggtatc gtgtctgtta
      481 aggccaaaga ccttggaact caaaaagaac aaactattgt catccaatcg aactcaggtt
      541 tgactgacga agaaatcgac cgcatgatga aagatgccga agcaaacgct gaagctgata
      601 agaaacgtaa agaagaagta gaccttcgta atgaagtaga ccaagcaatc tttgcgactg
      661 aaaagacaat caaggaaact gaaggtaaag gcttcgacgc
//
LOCUS       DAVAAL010000237          675 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVAAL010000237 DAVAAL010000000
VERSION     DAVAAL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.240"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..184
                     /locus_tag="VQM60_002066"
     CDS             <1..184
                     /locus_tag="VQM60_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET1081869.1"
                     /translation="NVAETLLLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEV
                     RNLACKHHNFKCDYCD"
BASE COUNT          227 a           91 c          115 g          242 t
ORIGIN      
        1 gaatgtagct gaaactcttc ttttaaacac tgaactacct ataaaagaga tagcagaatc
       61 tgttggatat tcatctgcca gcaaattttc tatctattat aaaagataca agggaaaact
      121 tccaagtgaa gtccgtaatt tagcttgcaa acatcacaat tttaaatgcg attattgtga
      181 ttaaagtaat tttttacaag ccatgttttt atatgaattt aagtgtatat gtgcatatat
      241 agtctgaata aatttgattt gaaaaatgga taaaaaaata aaggcagtcc gaagaccgcc
      301 aatatttatc tctctgcacc tttgctatgg tctttcttat ttgacttaga tttgtcatct
      361 gtcttaaagc tttttatttg ccctaatatg gactttttat ccttgtcttg actctttact
      421 tgttcttttg cttttaagag cttagaagac aaaaagagat attatattaa aacaatattt
      481 ctccggtact gggactggga cgggactaaa aatctgaaaa cgtcctataa ttaggtggat
      541 ttagtgatat tataaaaaat agaatctagt attttgaatg gttttggata tattgtacgt
      601 ttggaaaaga agaatgggag taattttaaa ctaacaaaaa atgccttgaa atcagtgttt
      661 cagagcattt ttggg
//
LOCUS       DAVAAL010000238          650 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVAAL010000238 DAVAAL010000000
VERSION     DAVAAL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.241"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
BASE COUNT          304 a           71 c          104 g          171 t
ORIGIN      
        1 aaggaggtca gttttttaat ggcaaataca gcacagaatt taagataaaa ttagtaaaag
       61 aatatcatga atgaaaaata ggatatagag aataacagca atattaaaaa gatcaggact
      121 gataataaac aaaaagaaag tacaacgact agttcaaaag ctaaaacttc aagtaaaaag
      181 ttattcaaga aaatctagaa aatactcatc ctacaaagga caggtaggaa aaatatcaga
      241 caacaaaata aaaagaaata tttattaccg aaaaaaaggg gaaatcgagt caagtaccaa
      301 caggctcatc tattccaaaa agatatttac tcgatggaaa aactcctaga attagtgcta
      361 caaatattga caatggtatc ttaggttact acgaagatat agatgataaa aattatagag
      421 tttttgaaaa ttttatatct gtttcattat taaattaccg atagacagta gaggtactcc
      481 agattggaat tacatggaaa gatatataga agacttaaaa ttaaaatgca atatagcaaa
      541 ttataatatt taaaacaaga acgattagaa ataaaaatct agtcgttttt tattttaaga
      601 aaggatacat gatggcaagt aaaagattaa gtattgaaga acagattgaa
//
LOCUS       DAVAAL010000239          637 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVAAL010000239 DAVAAL010000000
VERSION     DAVAAL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.242"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..328
                     /locus_tag="VQM60_002067"
     CDS             <1..328
                     /locus_tag="VQM60_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET1081870.1"
                     /translation="NYRDITGAFVGTLTLQNLSNLQFSKAFVSCNGIQNGALATFSEE
                     EGEAQRIALNNSNKKYLLADHSKFNKFDFYTFYNISNLDTIVSDSKLSDSILFKLSKH
                     IKVIKP"
BASE COUNT          218 a          108 c           97 g          214 t
ORIGIN      
        1 aaattatcgc gatattacag gtgcttttgt tggcacattg accctacaaa atctctctaa
       61 tctccaattt tctaaagctt tcgttagctg taatggtatt caaaacggag ctctagctac
      121 ttttagcgag gaagagggag aggctcaacg catcgcttta aataattcta ataaaaaata
      181 tttactcgca gatcatagta agttcaataa gtttgatttt tatacttttt ataatatatc
      241 aaatcttgat actattgttt cagattctaa actaagtgat tcaatccttt ttaagctatc
      301 taaacacatt aaagtcatca agccttaata aactgaaaga gcctgggtaa caactcaatt
      361 tcagaagaaa aaaagtacat cttcccacaa taaaacgcat agtatcaggt gttgaaacac
      421 ctgatactat gcgtttttct atatcaaaga ctttttgccc aattcctcaa agctatacat
      481 tgagaaatct gcgactcgct tcctagttta aagttgctct ttgattttca ttgagtataa
      541 gcaacgtgat tagataagag ttttacagag tactaaatat aagatttaac ttttttgaga
      601 aattggttac gattgttgaa acggcataaa atctgaa
//
LOCUS       DAVAAL010000240          626 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVAAL010000240 DAVAAL010000000
VERSION     DAVAAL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.243"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..>626)
                     /locus_tag="VQM60_002068"
     CDS             complement(<1..>626)
                     /locus_tag="VQM60_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET1081871.1"
                     /translation="AKIQEQGKLTKELEEAILVAEKLADVEELYLPYKEKRRTKATIA
                     REAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDILVEALSEDVTLRSM
                     TYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRTLALNRGEKLGVLKI
                     GFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRIRTEL"
BASE COUNT          185 a          150 c          113 g          178 t
ORIGIN      
        1 taattctgtc cgaatacgac gctcaatagc aggcaagacc tttttcttaa cggattgctg
       61 aacaacttca tcaatataag catttttcac cttgaaacga gtagcaaaga aggcaagaat
      121 acggtccgtc gcatgttcaa aaccgatctt caagacacca agtttctccc cacgattgag
      181 agccaaggta cgatagcctt gcatagttcc aactgtctct gaaaaatcat aataaatctg
      241 aaaaacctgc ttttcatcaa gactttcatc cttggcttga gaagtgagtt tagagtgtct
      301 cagcacttcc tgataagtca tagaacgcaa ggtcacatct tccgataagg cttcgaccaa
      361 aatatcaact gcaccggtca aggcttcctt gccagtcgca aatccttcac agacgaactt
      421 ttcagcttct ttctctaagt caactatatt ctgcaaaatc aagcgagcaa gaggaaagag
      481 tccagcttca cgggcaatgg ttgccttggt acgacgcttt tccttataag gaagatagag
      541 ttcttcaacg tctgctaatt tttcggcaac taagatagct tcttccaatt ccttggtcaa
      601 cttaccttgt tcttgaatct tagcta
//
LOCUS       DAVAAL010000241          620 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVAAL010000241 DAVAAL010000000
VERSION     DAVAAL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.244"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..587
                     /locus_tag="VQM60_002069"
     CDS             <1..587
                     /locus_tag="VQM60_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET1081872.1"
                     /translation="QYTRCLDIIFPELDKIVGKHSEYTYQLLTRYPNPQKRLEAGFDK
                     LIEIKRLTASKIQDILSVAPRSIGTTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAE
                     FNSVITTVTGIGGRLGAVILAEIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMI
                     KRGSPHLRWALIQAAKACARFSPAFKAYLKTKLE"
BASE COUNT          176 a          139 c          127 g          178 t
ORIGIN      
        1 tacaatacac tcgttgtctt gatatcatct ttcctgagtt ggataaaatc gttggaaagc
       61 attcagaata tacctaccaa ctcttgacac gctaccctaa tcctcagaaa aggcttgagg
      121 caggatttga taagctgata gaaattaagc gattgaccgc ttctaaaatt caggatatcc
      181 tctcagtcgc acctcgttct atcggaacaa catctcctgc tcgtgaattc gaaatcatcg
      241 aaatcatcaa acattacaag aggctcattg acaaggcgga aacatgtgtc aatgacttga
      301 tggctgagtt caactcggtc atcacgacgg ttactgggat tgggggtcgt ttaggggcgg
      361 tcattttagc cgagattcga aatattcatg cctttgataa tcctgctcaa ttacaagctt
      421 tcgctggact ggattcttct atttatcagt caggtcagat tgatttagct ggaagaatga
      481 tcaaacgggg ttcccctcat ctgcggtggg cactcataca agctgccaaa gcatgcgctc
      541 gcttttcacc tgcttttaag gcctatctta agactaagtt agaataagga aaacattaca
      601 atgtagccat catccacctt
//
LOCUS       DAVAAL010000242          605 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVAAL010000242 DAVAAL010000000
VERSION     DAVAAL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.245"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            165..383
                     /locus_tag="VQM60_002070"
     CDS             165..383
                     /locus_tag="VQM60_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET1081873.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          207 a           71 c          111 g          216 t
ORIGIN      
        1 ttttcattga gtataacaca ttgttagaag ttggtttaaa tttcctaatc agtttgttca
       61 catttacctt cgatatatta tatcccatag ttaaggttgg tcatacagat gattatagcc
      121 atggagccgt aaaacttagt gtttctttag ttgacaaaga tgccatgaaa aaaatatctg
      181 taactgtaat aggatatttt gaaataaata tagatgaaaa tatcaccgat attctatacg
      241 taaatggtac tgctattctt tatccttatt tacgttctat tgtttcaata gtttcggcaa
      301 ttgatagcag tgaagcaatg ttgctaccta ccattaatgt tttagagtta ctagataaat
      361 ctcaaccttt tgaagaagaa taatttatta gctcactaaa ttgagggtaa ggaaaagtaa
      421 aagcagtaag aaaaatgtct tgcattatac agcaactttt tgggaatgag tagatggatt
      481 gaataaaatt tgattaagag tggatgattt atctgtagat tattattgga cagttagtct
      541 tgaagtagtc taagaattag gttataatca gtagaagcct tgctaataat gaggaggtta
      601 gttta
//
LOCUS       DAVAAL010000243          604 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVAAL010000243 DAVAAL010000000
VERSION     DAVAAL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.246"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..>604
                     /locus_tag="VQM60_002071"
     CDS             <1..>604
                     /locus_tag="VQM60_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET1081874.1"
                     /translation="YSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQKFSPDKK
                     QASTNFKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIR
                     LIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERG
                     TNENHNRLIRRWLPKGTKKMTPKEVAFIEKWINNYPKKCL"
BASE COUNT          204 a          129 c          120 g          151 t
ORIGIN      
        1 tttattctgc cgactatgct caacagtctt atgaaaacaa tcgcaagcgc tcggtcaaga
       61 aatcaagctt gaccaaggaa ctaaaggaaa agattctcca ctatcataac caaaaatttt
      121 cgcctgataa gaaacaggct agcaccaact ttaaacctgc tggtcaatcc atcgaacagc
      181 ggtctgaagc tatcaatctt cgcttggaga atgggcatta tgagattgat acggttctac
      241 ttacgagagc gaaaaactac tgcttgcttg tcttgacgga tcgaaagagt agacatcaga
      301 tcatccgatt gattccaaat aaaagtgctg aggtggtcaa tcaggctcta aaactcatct
      361 taaaacaaca caagattctt tccatcacgg cagataatgg aacggaattc aatcgcttgt
      421 ttgatgtatt ttctgaggag cacatctatt atgcgcaccc ctatgcctct tgggaaaggg
      481 gaactaatga gaatcacaac aggctcattc gtagatggtt acctaaggga accaagaaaa
      541 tgactcccaa agaagtcgca ttcatcgaaa agtggattaa caactatcct aaaaaatgct
      601 tgga
//
LOCUS       DAVAAL010000244          594 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVAAL010000244 DAVAAL010000000
VERSION     DAVAAL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.247"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..>594
                     /locus_tag="VQM60_002072"
     CDS             <1..>594
                     /locus_tag="VQM60_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HET1081875.1"
                     /translation="KAEATRLEKIKTDRKKAEEAKRKAAEEDKVKEKPAEQPQPAPAP
                     KPEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGSMATGWLQN
                     NGSWYYLNANGDMATGWLQNNGSWYYLNANGSMATGWLQYNGSWYYLNANGDMATGWL
                     QNNGSWYYLNANGSMATGWLQNNGSWYYLNANGSMAT"
BASE COUNT          222 a          128 c          132 g          112 t
ORIGIN      
        1 aaaaagctga ggctacaagg ttagaaaaaa tcaagacaga tcgtaaaaaa gcagaagaag
       61 ctaaacgaaa agcagcagaa gaagataaag ttaaagaaaa accagctgaa caaccacaac
      121 cagcgccggc tccaaaacca gaaaaaccag cacaaccatc tactccaaaa acaggctgga
      181 aacaagaaaa cggtatgtgg tacttctaca atactgatgg ttcaatggcg acaggatggc
      241 tccaaaacaa tggctcatgg tactatctaa acgctaatgg ttcaatggca acaggatggc
      301 tccaaaacaa tggttcatgg tactacctaa acgctaatgg tgatatggcg acaggatggc
      361 tccaaaacaa tggttcatgg tactacctaa acgctaatgg ttcaatggcg acaggatggc
      421 tccaatacaa tggctcatgg tactacctaa acgctaatgg tgatatggcg acaggatggc
      481 tccaaaacaa tggctcatgg tactacctaa acgctaatgg ttcaatggcg acaggatggc
      541 tccaaaacaa tggctcatgg tactacctaa acgctaatgg ttcaatggcg acag
//
LOCUS       DAVAAL010000245          551 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVAAL010000245 DAVAAL010000000
VERSION     DAVAAL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.248"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..>551
                     /locus_tag="VQM60_002073"
     CDS             <1..>551
                     /locus_tag="VQM60_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET1081876.1"
                     /translation="NFINQKDKKGSIEDSVLNIVFVTTRTKYCSQILQLLLKYFDKNP
                     NKISEVYSILEANYGLVTEREYIDYTLAENTISELANLDLTKSYNQELIVTILKQFLK
                     IEIERTEAHEEKFTFGRYKVPDSEKLKQYHRSILKLLANLYNIGSCETRFYIEKMLYD
                     YRRKILTYSESHRNIIFGDLRNI"
BASE COUNT          213 a           60 c           94 g          184 t
ORIGIN      
        1 taattttatt aatcagaagg ataaaaaggg atctattgag gattcagttt taaatattgt
       61 atttgtaact acacggacaa aatattgtag tcaaattctc caattattgt taaagtactt
      121 tgataaaaat cctaataaaa tttctgaggt ttatagtatt cttgaagcaa actacgggtt
      181 ggttacagaa agagaatata ttgattatac cttagctgaa aataccattt ctgaacttgc
      241 taatcttgat ttgacgaaaa gttataacca agaattaatc gtgacaattt tgaagcagtt
      301 tcttaaaatt gaaatcgaaa gaacagaggc tcatgaagag aaatttactt ttgggcgcta
      361 taaagtcccg gatagtgaaa aattaaaaca gtatcataga agtatactaa aacttttagc
      421 gaatttgtat aatataggga gttgtgaaac tagattttat attgagaaaa tgctatatga
      481 ttacagaaga aaaattttga cctattcaga aagtcatcgt aatattatct ttggagattt
      541 gagaaatatt a
//
LOCUS       DAVAAL010000246          476 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVAAL010000246 DAVAAL010000000
VERSION     DAVAAL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.249"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..180
                     /locus_tag="VQM60_002074"
     CDS             <1..180
                     /locus_tag="VQM60_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081877.1"
                     /translation="SVDFVNKELGIDLSIEIDKLIKQGEKPSVIAQIKKFQDQVNKDN
                     EYREMMKNKKMDQER"
     gene            254..427
                     /locus_tag="VQM60_002075"
     CDS             254..427
                     /locus_tag="VQM60_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081878.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
BASE COUNT          234 a           74 c           70 g           98 t
ORIGIN      
        1 tcagtagatt ttgtaaacaa agaacttggt atagatttat ccatagaaat agataagcta
       61 ataaaacagg gagaaaaacc aagtgtaata gcacagatta aaaagttcca agaccaagtc
      121 aataaagata atgaatacag agaaatgatg aaaaacaaga aaatggatca agaaagataa
      181 gacctggtaa aaatatctta tccaagctat aatatgacca aacaactaaa gtcagatcta
      241 ggaggtaata gagatgaata aaaggcaaga gctaatagat gaactcataa aagcaaatga
      301 agatggaaca tacaaaacct ataaaagcac agaagaaata aaagcaatga acaatgaaga
      361 aatacagatt atatattcca acatgaaaaa ctatctatca gacaaaagaa cacacataaa
      421 ctattaaccc caattattca cccctaatct aaaaaccatc cagaatcctt gcctta
//
LOCUS       DAVAAL010000247          473 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVAAL010000247 DAVAAL010000000
VERSION     DAVAAL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.250"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            complement(<1..>473)
                     /locus_tag="VQM60_002076"
     CDS             complement(<1..>473)
                     /locus_tag="VQM60_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET1081879.1"
                     /translation="CRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQL"
BASE COUNT          154 a           92 c          109 g          118 t
ORIGIN      
        1 gaagttgatt aaaccgttct aaaacaggtg tgataaagct gtctgcctct tcagaacaat
       61 aacgattacc aggacgaagc tgggcattga aacaataacc tgtcttcccc tcgaaagcat
      121 aaagaggatg atagccatga gcacgatagt gggcgttata agcaacacct tcttgcttgc
      181 cataagttgt gaaatgggta gaatcgatat ctacaatgag ttggtttagc tggtgaaact
      241 gtaaaaagaa ttcgaccaat tcaaggttga ggcatcgcaa actatggact gtttcctcgt
      301 cagttctgga aagaaaacgg gataaggttg gctgtgaagc aagctgccct ccttccaaca
      361 attttggaaa gtaggcatca gctgacaatt ctttacaagc atagtccgtt ccataacctg
      421 ttaacagttg aaagaggaac tggacaagga tatctgaatc cgaataacga cag
//
LOCUS       DAVAAL010000248          472 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVAAL010000248 DAVAAL010000000
VERSION     DAVAAL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.251"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..>472
                     /locus_tag="VQM60_002077"
     CDS             <1..>472
                     /locus_tag="VQM60_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET1081880.1"
                     /translation="EQKTLFILSYLKKIRLESLEENQEFNKLLKITDITNTDFKSAVE
                     KLHERELCNIYNDKIVKIADQSLDDYIVIKFLINKKISILEILHELYPVNDQRVVQIL
                     NQCSNFIRKESDLEGVSDAVKSYYYNESNFESDELKEKFLIQFGVLLPLEAISHV"
BASE COUNT          195 a           51 c           74 g          152 t
ORIGIN      
        1 gaacaaaaga ctctatttat tttatcttac ttaaaaaaga ttagactgga aagtttagaa
       61 gagaaccagg aattcaataa attactaaaa ataactgaca taacaaatac tgactttaag
      121 agtgcagtgg agaagcttca tgaaagagag ttgtgtaata tttataacga caaaattgta
      181 aagatagcag atcaatcatt agatgattat atagttatca agtttttaat taacaagaaa
      241 atatcaattt tagaaatttt acatgagtta tatccagtaa acgaccaaag agttgttcaa
      301 atactaaatc aatgttccaa ttttattagg aaagagagtg atttagaagg cgtttctgat
      361 gcagttaaat catattatta caatgaaagt aattttgaaa gtgatgaact taaagaaaag
      421 tttttaatac aatttggagt attacttcct ttagaagcaa tttcacacgt ta
//
LOCUS       DAVAAL010000249          469 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVAAL010000249 DAVAAL010000000
VERSION     DAVAAL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.252"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..>469
                     /locus_tag="VQM60_002078"
     CDS             <1..>469
                     /locus_tag="VQM60_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET1081881.1"
                     /translation="HQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAP
                     IRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTH
                     SHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAA"
BASE COUNT          151 a           94 c          109 g          115 t
ORIGIN      
        1 ttcaccaaga tttacttgat aataaaggtc gacaagttga ttttgaggct ttggataacc
       61 tgttggaacg actcaaggat gtctcaagtg ataaagtcaa gttagtggat gatattcttg
      121 ccttcttagc tccgattcgt catccagaac gtttaggaaa accaaatgcg caaattacct
      181 acactgatga tgagattcaa gtagccaagt tggcaggcaa gtacacaaca gaagacggtt
      241 atatctttga tcctcgtgat ataaccagtg atgaggggga tgcctatgta actccacata
      301 tgacccatag ccactggatt aaaaaagata gtttgtctga agctgagaga gcggcagccc
      361 aggcttatgc taaagagaaa ggtttgaccc ctccttcgac agaccatcag gattcaggaa
      421 atactgaggc aaaaggagca gaagctatct acaaccgcgt gaaagcagc
//
LOCUS       DAVAAL010000250          463 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVAAL010000250 DAVAAL010000000
VERSION     DAVAAL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.253"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
BASE COUNT          161 a           54 c           93 g          155 t
ORIGIN      
        1 actgttatag attcaataca ctataattta gtgtaattga gaaaggagaa atgattgtga
       61 ttgatgttgg ctaggttatt ttcaatgatt cctaccgtct caaatcttgt cagtaaggaa
      121 aaataaattc ttcaaaagta gagattacaa ggcttgttta agaaagaatt caaagacctt
      181 gacaaataaa aataaaatgg ttattataag aaatggtcta aaatagatga tgatactttt
      241 cgaaaatctt tttatgtcag ctcagctttg ccttgctgtg ttttgagcaa gctacggtta
      301 gcttccgagt ttgattttca tttactagaa atgaaactga tgagagatat cagtagacat
      361 ttgagtcagg atattatgga aaatgataaa aagagctcgt gagattggca tatcagacta
      421 ctaaagtatt gagtttgtta ggattttagc gactagttag ctg
//
LOCUS       DAVAAL010000251          448 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVAAL010000251 DAVAAL010000000
VERSION     DAVAAL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.254"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            202..327
                     /locus_tag="VQM60_002079"
                     /pseudo
     CDS             202..327
                     /locus_tag="VQM60_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          186 a           73 c           68 g          121 t
ORIGIN      
        1 aaacacagta tatcattttt aaattttaag tcaaggaaaa ttaacccact ttaatttctt
       61 ttttaacccg tgttaaaaaa gaaattaaag tgggttaaaa aagaaattaa ctgagtaaaa
      121 actctcgaaa ttcaattcgc tatattatca ctcaacttga acaaatacca gaaccacaaa
      181 aaacagatgt aaaggaaaaa tatggactta aaatttgaag gggttgattt agaatacaaa
      241 aaagctaaaa acaatcttcc agagtctttc tgggaaacct actcagcatt tgcaaataca
      301 aatggcggga aaataattct cgggatagat gaaaaaaaca ttgacaccta ccaaagagtc
      361 aatagactcc ctgcgaaatt atgaagcaag taaacaatta actgatacac gatttaagcg
      421 tcttgttggt gttcagcaca cgactttt
//
LOCUS       DAVAAL010000252          415 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVAAL010000252 DAVAAL010000000
VERSION     DAVAAL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.denovo.255"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            <1..>415
                     /locus_tag="VQM60_002080"
     CDS             <1..>415
                     /locus_tag="VQM60_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HET1081882.1"
                     /translation="KSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQA
                     PSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLL
                     SDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLS"
BASE COUNT          153 a           64 c           54 g          144 t
ORIGIN      
        1 taaaatcaat ttattggaat tttgggcaaa ataagccaga gaaatccttt aggtatatag
       61 atacgtctag tattgataga aaaaagaaca taatcaacta caaaaatcta caatatcttt
      121 cacctgaaca agcgccttcc cgtgctagaa aattagtttc gcagaatagt gtcttatttt
      181 caacagttag accatatcta aaaaatattg ctgtagttag agaacttaaa gagtatttga
      241 tagctagtac agcatttatt gttttggata ctttacttaa cgaaacatat ttgaaatatt
      301 atttattatc agataatttt atcaatcgtg ttaataataa atctactgga acaagttatc
      361 ctgcaatcaa tgattataat tttaatctat tattaattgc tctcccccca ctatc
//
LOCUS       DAVAAL010000253        20186 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.guided.024, whole genome shotgun sequence.
ACCESSION   DAVAAL010000253 DAVAAL010000000
VERSION     DAVAAL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.guided.024"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            27..1412
                     /locus_tag="VQM60_002081"
     CDS             27..1412
                     /locus_tag="VQM60_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET1081883.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVQAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            1415..1567
                     /locus_tag="VQM60_002082"
     CDS             1415..1567
                     /locus_tag="VQM60_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HET1081884.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            1590..2852
                     /locus_tag="VQM60_002083"
     CDS             1590..2852
                     /locus_tag="VQM60_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HET1081885.1"
                     /translation="MEGFLLNEQTWLQQLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLFEALERFNPDAPLEMLFDYVRIRFPTTDVQHVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDALVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKKGYLSTIPVDRYPKKRYNGR"
     gene            2797..3009
                     /locus_tag="VQM60_002084"
     CDS             2797..3009
                     /locus_tag="VQM60_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HET1081886.1"
                     /translation="MCQPYQLTDIQKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKE
                     VMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            3058..3141
                     /locus_tag="VQM60_002085"
     CDS             3058..3141
                     /locus_tag="VQM60_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HET1081887.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            3266..4003
                     /gene="erm(B)"
                     /locus_tag="VQM60_002086"
     CDS             3266..4003
                     /gene="erm(B)"
                     /locus_tag="VQM60_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HET1081888.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            complement(4260..5507)
                     /locus_tag="VQM60_002087"
     CDS             complement(4260..5507)
                     /locus_tag="VQM60_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HET1081889.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            5687..5908
                     /locus_tag="VQM60_002088"
     CDS             5687..5908
                     /locus_tag="VQM60_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081890.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            6025..6522
                     /locus_tag="VQM60_002089"
     CDS             6025..6522
                     /locus_tag="VQM60_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HET1081891.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            6497..7003
                     /locus_tag="VQM60_002090"
     CDS             6497..7003
                     /locus_tag="VQM60_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HET1081892.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            6987..9434
                     /locus_tag="VQM60_002091"
     CDS             6987..9434
                     /locus_tag="VQM60_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET1081893.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            9437..11614
                     /locus_tag="VQM60_002092"
     CDS             9437..11614
                     /locus_tag="VQM60_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET1081894.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKAERASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            11611..12612
                     /locus_tag="VQM60_002093"
     CDS             11611..12612
                     /locus_tag="VQM60_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HET1081895.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            12609..13541
                     /locus_tag="VQM60_002094"
     CDS             12609..13541
                     /locus_tag="VQM60_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HET1081896.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            13786..13902
                     /locus_tag="VQM60_002095"
     CDS             13786..13902
                     /locus_tag="VQM60_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HET1081897.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            13918..15837
                     /gene="tet(M)"
                     /locus_tag="VQM60_002096"
     CDS             13918..15837
                     /gene="tet(M)"
                     /locus_tag="VQM60_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HET1081898.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            16201..16446
                     /locus_tag="VQM60_002097"
     CDS             16201..16446
                     /locus_tag="VQM60_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081899.1"
                     /translation="MRQIGVSYSGFVDESYTLLSLFDDVEQIEKDNRLQTAIDVVREQ
                     FGFLAIQKGTVLTEGSRNIERSKLIGGHSAGGLEGLK"
     gene            16443..16778
                     /locus_tag="VQM60_002098"
     CDS             16443..16778
                     /locus_tag="VQM60_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imsC-like protein"
                     /protein_id="HET1081900.1"
                     /translation="MKQEKNTVQFSEIRSKGCNDIEMLERFLHGIVETATSKLRQRKL
                     KTTEISIRLVHAKSENRLPLEFTFSIKPTSSSVIIYTEVINRFKECYTGGGIQGFTIQ
                     FDKNTLASA"
     gene            16791..17159
                     /locus_tag="VQM60_002099"
     CDS             16791..17159
                     /locus_tag="VQM60_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081901.1"
                     /translation="MIDRSYLPFQSAREYQDTKMQKWMGFFLSEHASALSDDTNKVTY
                     MSDLSLEKKLLLLSQVYAGQLRTRIQVIEKNKRVSYTGTIPSLTKDFILIKTTTGHIN
                     LKLKDIISIELVEEVLYESA"
     gene            17146..17445
                     /locus_tag="VQM60_002100"
     CDS             17146..17445
                     /locus_tag="VQM60_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081902.1"
                     /translation="MNQLEFQRNHLQMDYYSESYQDFERDFYRYSNMNIPLTFLTDDI
                     LKTMATSRKNYFVLNKEKSRDNRDHFFIFEVSTVDENPLIYHYTYKKTTIYLAEK"
     gene            complement(17563..19026)
                     /gene="msr(D)"
                     /locus_tag="VQM60_002101"
     CDS             complement(17563..19026)
                     /gene="msr(D)"
                     /locus_tag="VQM60_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(D)"
                     /protein_id="HET1081903.1"
                     /translation="MELILKAKDIRVEFKGRDVLDINELEVYDYDRIGLVGANGAGKS
                     TLLRVLLGELTPPGCKMNRLGELAYIPQLDEVTLQEEKDFALVGKLGVEQLNIQTMSG
                     GEETRLKIAQALSAQVHGILADEPTSHLDREGIDFLIGQLKYFTGALLVISHDRYFLD
                     EIVDKIWELKDGKITEYWGNYSDYLRQKEEERKSQAAEYEQFIAERARLERAAEEKRK
                     QARKIEQKAKGSSKKKSTEDGGRLAHQKSIGSKEKKMYNAAKTLEHRIAALGKVEAPE
                     GIRRIRFRQSKALELHNPYPIVGAEINKVFGDKALFENASFQIPLGAKVALTGGNGIG
                     KTTLIQMILNHEEGISISPKAKIGYFAQNGYKYNSNQNVMEFMQKDCDYNISEIRSVL
                     ASMGFKQNDIGKSLSVLSGGEIIKLLLAKMLMGRYNILIMDEPSNFLDIPSLEALEIL
                     MKEYTGTIVFITHDKRLLENVADVVYEIRDKKINLKH"
     gene            complement(19146..>20186)
                     /gene="mef(A)"
                     /locus_tag="VQM60_002102"
     CDS             complement(19146..>20186)
                     /gene="mef(A)"
                     /locus_tag="VQM60_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HET1081904.1"
                     /translation="LGPAIGVLVDRHDRKKIMIGADLIIAAAGAVLAIVAFCMELPVW
                     MIMIVLFIRSIGTAFHTPALNAVTPLLVPEEQLTKCAGYSQSLQSISYIVSPAVAALL
                     YSVWDLNAIIAIDVLGAVIASITVAIVRIPKLGNQVQSLEPNFIREMKEGVVVLRQNK
                     GLFALLLLGTLYTFVYMPINALFPLISMEHFNGTPVHISITEISFAFGMLAGGLLLGR
                     LGGFEKHVLLITSSFFIMGTSLAVSGILPPNGFVIFVVCCAIMGLSVPFYSGVQTALF
                     QEKIKPEYLGRVFSLIGSIMSLAMPIGLILSGFFADKIGVNHWFLLSGILIIGIAIVC
                     QMITEVRKLDLK"
BASE COUNT         6493 a         3740 c         4005 g         5948 t
ORIGIN      
        1 gctaggtaga aaggagactt tttcgcatga aacagcgtgg taaaaggatt cgcccatctg
       61 gtaaagattt agtctttcat tttacgatag cgtcactcct gcctgttttc ctgctggttg
      121 tcggactgtt tcatgtgaag acaatccagc agatcaactg gcaggatttt aacctatcac
      181 aagcagataa gattgacatt ccctatttaa ttatcagttt cagtgtcgca attcttatct
      241 gcttgctggt agcgtttgta ttcaaacggg ttcgctatga tacggttaaa caactttacc
      301 accgtcaaaa actggcaaag atgatacttg aaaacaagtg gtatgaatct gaacaggtca
      361 aaacagaggg tttctttaaa gatagtgctg gtcgtacaaa ggaaaagata acctacttcc
      421 ctaaaatgta ttatcgactt aaaaatggct tgatacagat acgggtggaa atcacgctgg
      481 gaaaatatca agaccaactc ttacacttgg aaaagaaatt agagagtggc ttgtactgtg
      541 agctgacgga taaagagtta aaggattcct atgtggaata tactttgctc tatgacacca
      601 tagccagtcg tatttctatt gatgaagtac aagctaaaga tggtaaactt cgcttaatga
      661 aaaacgtatg gtgggaatat gataagctcc ctcatatgtt gattgctggt ggtacaggtg
      721 gcggtaaaac ttactttata ctgacactga ttgaagcctt gcttcataca gattcaaaac
      781 tgtatattct tgacccgaaa aatgctgacc ttgcggactt aggttctgtg atggcaaatg
      841 tctactatag aaaagaagac ttgctttctt gcattgaaac attctatgaa gaaatgatga
      901 aacgtagtga ggaaatgaag cagatgaaga actataagac tggcaaaaat tatgcttact
      961 taggtctccc ggcacacttc ttaatctttg atgaatacgt cgctttcatg gaaatgctgg
     1021 gaacaaaaga aaacaccgca gttatgaata agctgaaaca gattgtcatg ttaggtcgtc
     1081 aagctggctt ctttctaata ctggcttgtc aacgtccaga cgcaaaatat ttaggcgacg
     1141 gaatccgtga tcagtttaat ttcagagtgg ctttaggtcg tatgtctgaa atgggctatg
     1201 gcatgatgtt tggcagtgac gtacaaaagg atttcttctt aaagcgaatc aaaggtcgtg
     1261 gctatgttga tgtaggaaca agtgtcatat cagagtttta tactcccctt gtaccaaaag
     1321 gatatgattt cttggaggaa attaaaaagt tatccaacag cagacagtcc acgcaggcga
     1381 cgtgcgaagc ggaagtcgca ggtgtggact gatcttgctg gctggtgtgg caatagccac
     1441 gccagcactt aaccccccgt atctaacagg ggggtacaaa tcgacaggaa acagtcaaaa
     1501 aaacattaga aaatcctttg gttacaaggg atttacaaaa tttcagcgta tgtcaaatgg
     1561 gctttaaaag ttgacatacg cctttttgat tggagggatt tttactgaat gaacaaactt
     1621 ggttacagca gttaaaagaa aaacgcttgg cttatggact atctcaaaat cgtttagcgg
     1681 ttgcgactgg tattacaagg cagtatctaa gcgatattga aacaggaaaa gtcaagccat
     1741 cagaggattt acagcaatct ctttttgaag ctctggaacg cttcaatccc gacgctcccc
     1801 ttgaaatgct ctttgattat gtaaggattc gctttcccac aacggacgta cagcatgtgg
     1861 tcgaaaacat cttacaactg aaactgtcct attttcttca tgaggactat ggtttctatt
     1921 cttattcaga gcattatgct ttaggcgata tattcgttct ctgctcccac gaactggaca
     1981 aaggagttct ggtggaattg aaaggtcgtg ggtgtcggca atttgaaagc tatcttctgg
     2041 ctcaacaaag aagctggtat gagttcttta tggacgcttt ggtggctggc ggtgtgatga
     2101 aacgccttga ccttgccatt aacgataaga cagggatttt gaatatccct gtactcactg
     2161 aaaagtgcca acaggaagaa tgtatctccg tcttccgcag ttttaaaagc tatcgcagtg
     2221 gcgaactggt acgcaaagag gaaaaggaat gtatgggaaa caccctctat atcggttcat
     2281 tacaaagtga agtttatttc tgtatctatg aaaaggacta cgagcagtac aagaaaaatg
     2341 atattcccat tgaagacgca gaagtaaaaa accgttttga gattcgattg aaaaatgagc
     2401 gtgcctatta tgcagtccgt gatttactcg tctatgacaa tccagagcat accgccttta
     2461 aaattatcaa tcggtatatc cgttttgtag ataaagacga ttccaaacct cgttctgatt
     2521 ggaaactgaa tgaagaatgg gcttggttta ttgggaacaa tcgtgaacga ttaaaactaa
     2581 ccacaaaacc agagccttac tccttccaaa ggacgctgaa ctggctatct catcaagttg
     2641 ccccgacctt aaaggttgcg attaaacttg atgaaatcaa ccagacgcag gttgtaaaag
     2701 acattctcga ccatgcgaaa ctgacagacc gacacaagca gattttgaag caacagtcag
     2761 taaaagaaca ggacgtgata acaacaaaaa aaggatatct gtcaaccata ccagttgaca
     2821 gatatccaaa aaaaagatat aatgggagat aagacggttc gtgttcgtgc tgacttgcac
     2881 catatcataa aaatcgaaac agcaaagaat ggcggaaacg taaaagaagt tatggaaata
     2941 agacttagaa gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat
     3001 aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg
     3061 ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca gactaaaaac
     3121 agtgattacg cagataaata aatacgttag attaattcct accagtgact aatcttatga
     3181 ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag
     3241 atgtaatcac ttcaggagag attacatgaa caaaaatata aaatattctc aaaacttttt
     3301 aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac
     3361 cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa
     3421 acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa
     3481 actgaatact cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa
     3541 acagaggtat aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa
     3601 agtggttttt gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa
     3661 gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca
     3721 gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt
     3781 aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta
     3841 ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca
     3901 tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc attacttatg agcaagtatt
     3961 gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt
     4021 ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta gatatactac
     4081 tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg tatcgataag
     4141 gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct aagtgggata
     4201 tctgtcaact ggtatggttg actaaaaata cttcctacga aaatgtaggg ggtatttttt
     4261 tacgaaaaaa tacaatcgat tcttaaaaag aaaaattttt gattggcaaa accataacaa
     4321 gttcgtttta gggttttgat tttgcgattg atgccttcta aaggaccatt agagtattca
     4381 aatttagcgc tatttaagac atattttctg ttttgacgaa gggtttgaat agcagtatcc
     4441 atttctgtat tggttttttg gtagtctaag atggttgact ctagtaattc actattgcgc
     4501 tcgtttaggg ctttcgtgat atcttggtaa gtttggtata cttcagcgaa cttggaaaat
     4561 ttactagtaa tgagatcaac agcattttgg cgagtcatat attgtttaac gccgcgaaga
     4621 aaaactactt cttcagggtg aagatcttca gcttttttat ggaatagctt ccaatgtgac
     4681 ttcataattt tatattcttg gctctgttta tcaagttgct ttaggataga gatacgacaa
     4741 ttgtccaaag cgcgaccagc taattgtaca aggtggaagc gatcaataat gatattggca
     4801 ttagggaaaa ggcgatagat aaaactttga tattgagcat ttaaatcaat tacaactgat
     4861 tgaacgcatt cgcgttcggc ttttgaataa cgactttcaa aataatcaac aatggtaggt
     4921 gatagacgat cctgtaactt tgtgacaatt tggtgggttt cagcgtcaca acagataaag
     4981 gacatcacag acttaattga acgaaactcg tcaaaacata gatgcttagg caacttagcc
     5041 acacgatagt gtggttccat gcgctctaag attgttcgac gaacactgct aggagagcag
     5101 tgacacattt cagcaataag ctgaccagat aagcctttac gagctaaaag catgatttga
     5161 tttttgagat cactggataa ggtttgattt tctttggtta aattagtaat agcaccaaaa
     5221 gtagtatggc atgatttaca tttatagcgt tgtttacgaa gctctagttc atatcttctc
     5281 ccatttaaac ttgccagtcg tacatgagtt ttgcgaaagc catccttatt aactgtggga
     5341 aagccacagt tacgacaacg attaatcgga taagaaagag tagctgttat tagcgttata
     5401 tactctttaa cagaatcgtt gttgtgttca gcttcttcaa cagaaataat tttaatattt
     5461 ttatctttaa ttccaagaat atttaggata gaatcattat gggacatttg tttaaccttc
     5521 tttcatgatt tttgtggtga attgattgta taacgagggg acagcaaatg tcctcttttt
     5581 tgtataaaaa aatctggcat ggaatctcta tccataccag aaagtgtata ccccaaaaaa
     5641 ataactcaaa tacaaattca ttgaatatag agaggagaac atttttatga attttggaca
     5701 aaacctttat aactggtttc tatcaaacgc tcaatcactg gtgcttttag caatcgttgt
     5761 gattggcttg tatcttggct tcaagcgtga gtttagcaaa ctgattggct ttttaattat
     5821 tgcgattatt gcggttggct tagtcttcaa cgctgctgga gtaaaagaca ttttactaga
     5881 gctattcaat cgcattattg gtgcttaaat aaaaccgttc ttttgtggaa tataagtggt
     5941 tttcttatgt tccgcaaagg aatggtacac caaacgaagt gcggtaggga tttttgaatc
     6001 tctacaaaga aaggacgtga atatatggac gatatgcaag tctatattgc gaatttaggc
     6061 aaatacaatg agggcgaatt ggtcggtgcg tggtttacct ttcccattga ctttgaggaa
     6121 gtcaaagaga aaatcggctt gaatgatgaa tatgaggaat acgccattca tgactacgag
     6181 ttacccttta cggttgacga atacacttcc attggcgaac tcaatcgact atgggaaatg
     6241 gtatcggaat tacccgaaga attacaatcg gagctatctg ctctgctcac tcatttttca
     6301 agcattgaag aactaagcga acatcaagag gatattatca ttcattccga ttgtgatgat
     6361 atgtatgacg tggcacgcta ctacattgaa gaaacgggtg ctttaggcga agtaccagct
     6421 agtcttcaaa actatattga ttatcaagcc tatggtcggg atttagacct ttcaggaacg
     6481 tttatctcaa ccaatcatgg gatttttgaa atcgtctatt aaatctgtcg gtacattact
     6541 actggcagat tttctatttt acggggtggc tcaatcagct acccctattt tttatgaaag
     6601 gattgattac atgaagaaaa tacgaagcta taccagtatc tggtctgtgg aaaaggtact
     6661 gtattctatc aatgatttca gacttccgtt tcccataacc tttacgcaaa tgacatggtt
     6721 tgtcgtgtca ctctttgcag tgatgatact tggcaacttg ccccctcttt ccatgataga
     6781 gggagcattt ctcaaatact ttgggattcc tgtggctttc acatggttta tgtctacaaa
     6841 aacttttgat ggtaaaaagc cttatggatt tttgaagtct gtcattgctt atgcactgcg
     6901 accaaagctg acctatgcag gaaaaaaagt aacgcttggc agaaaccagc cacaagaagc
     6961 cattacagca gttaggagtg aattttatgg catatccaat taaatacatt gaaaacaatc
     7021 tcgtctggaa taaagacggg gaatgttatg cttactatga gcttgttcct tacaattact
     7081 catttctaag tccagaacag aaaatacaag tgcatgattc tttcagacag cttatcgcac
     7141 aaaatcgtga tggcaaaatt catgctttac aaatcagtac agaatccagc atacgttctg
     7201 cacaagagcg ttccaaaaat gaagtcactg gcaagctcaa agcggttgcc tatgacaaaa
     7261 tcgaccaaca gacagacgct ttaatatcca tgattggcga aaatcaagtg aactaccgtt
     7321 tctttatcgg ctttaagttg cttctcaacg atcaggagtt ttctatgaaa agtcttaccg
     7381 ttgaagcaaa aaatgctttg tctgattttg tctatgatgt gaaccataag ctgatgggcg
     7441 attttgttag tatgagtaat gatgaaatcc tgcgttttca gaagatggaa aagctcttag
     7501 aaaataaaat ctctcgtcgt ttcaaaatcc gcaggttaga taaggacgac ttcggctatc
     7561 tgattgaaca cctttacgga cagacaggca ctgcctatga agagtatgag taccatctat
     7621 caaagaaaaa gctggataat gaaacgctga ttaaatacta tgacttgatt aagcctactc
     7681 gctgtttggt ggaagaaaaa cagcgatatt tgaaaatcca gcaggaagat gaaaccgtct
     7741 atgtagctta ctttaccatt aacagcattg tcggagaact ggacttcccg tcctctgaaa
     7801 tcttctacta ccagcaacag caatttacat tcccgattga tacgtcaatg aatgtggaaa
     7861 ttgtagcgaa tcgtaaagcc ctatctactg tccgcaataa aaagaaagaa ctgaaagact
     7921 tggataacca cgcatggcaa agtgataatg aaaccagctc taatgtggcg gaagctctgg
     7981 aaagtgtgaa cgagctggaa accaatttag accaaagcaa ggaatctatg tataagctgt
     8041 cctatgttgt aagggtatca gcaaatgacc ttgacgaact taaacgtcgt tgtaatgaag
     8101 tgaaagattt ctatgatgat ttgagcgtga aactggtacg accttttggg gatatgctgg
     8161 gcttacatga agaattttta cctgccagca aaagatatat gaatgactat attcaatacg
     8221 tgacctctga tttcctcgct ggtttaggtt ttggtgctac tcaaatgctg ggtgaaaatg
     8281 aggggattta tgttggctac agcttagata ctggacgcaa tgtctatctg aaacctgctc
     8341 ttgccagtca aggggttaag ggttcagtaa ccaatgcgtt agcgtctgcc tttgtcggtt
     8401 cgctgggtgg tggtaaatcc tttgcgaata accttatcgt ctattatgca gtgctttatg
     8461 gggcacaagc agtgattgta gacccaaaag cagaacgtgg cagatggaaa gaaaccttgc
     8521 cagagatttc ccatgaaatc aatatcgtca ctctgacttc tgatgagaaa aacaaaggct
     8581 tacttgaccc ttatgtgatt atgaaaaatc ccaaagattc tgaatcactg gctattgata
     8641 ttctgacatt ccttacgggg atttcctctc gtgatgggga acgcttccca atccttagaa
     8701 aagccattcg tgcagtaacc aatagtgaag tacgagggtt gatgaaagtg attgaggaat
     8761 tacgggttga gaatacgcca ctaagtacca gtatagccga ccatatcgaa agttttacag
     8821 actatgactt tgcacattta ttattcagta atggttatgt ggagcagtct atcagcttag
     8881 aaaaacaact gaacattata caggttgcgg acttggtact tcccgacaag gaaacttcct
     8941 ttgaggaata taccactatg gagcttttat ccgttgctat gctgattgtc attagtacct
     9001 ttgctttaga ctttatccat acagaccgaa gcattttcaa gattgtagat ttagacgaag
     9061 catggagctt tttacaggta gcacaaggaa aaacactatc tatgaagctg gttcgggctg
     9121 gtcgtgctat gaacgctggg gtatatttcg tgacccaaaa tacagacgac ctcttagatg
     9181 aaaaactgaa aaataacctc ggcttaaaat ttgcatttcg ttccactgac cttaacgaga
     9241 ttaaaaagac cttagccttt tttggtgtag acccagagga cgaaaacaat cagaagcgat
     9301 tgcgtgattt ggaaaacggg caatgcctta tcagtgattt atatggtcgt gtcggtgtga
     9361 tacagttcca ccctgtattt gaagaactgc tccatgcctt tgataccaga ccacctgtgc
     9421 gaaaagaggt gtaaatgtga aaccatcaat agtaaacaga ataaaatcaa actggacgct
     9481 gaaacgtcta ggtaaagtgg caatgacagt ggctttcaca cttgtgattg ccatttttct
     9541 tttagccatg ctgggaacgg tggttcaagc tgcgggcttg gtagatgata cggtcaatgt
     9601 ggcaaatgaa tacagccgat acccacttga aaactatcaa ctggattttt atgtggataa
     9661 tagctggggc tggcttccgt ggaactggtc ggacgggatt ggaaaacagg tcatgtatgg
     9721 actatatgcc attaccaatt ttatttggac aatcagtttg tatgtttcca atgcgacagg
     9781 ttacttagta caggaagcct attccttaga cttcatttcc gctacagcag attccattgg
     9841 taagaatatg cagaccttag ctggtgtgag tgcaaacgga ttttcaacag agggtttcta
     9901 tgttggattc ctcttactct tgattttggt tcttggggtt tatgttgcct atacgggact
     9961 gataaagaga gaaaccacaa aggcaattca tgccattatg aattttgtgc tggtgtttat
    10021 cctatcggct tcctttattg cctacgctcc cgactacatt aaaaaaatca atgacttttc
    10081 atcagacatc agtaatgcca gtttatcact tggcacgaag attgtcatgc cccattccga
    10141 tagtcaaggc aaggacagcg tggacttaat cagagatagc ctgttttcca tacaggttca
    10201 gcaaccgtgg ctactgcttc aatacaacag ttcagacatt gaaagtatcg gtattgaccg
    10261 tgtggaaagc ctgctctcca ccagcccaga ttccaacaat ggcgaagaca gagaaaaaat
    10321 tgttgcggaa gaaattgaag acagaagcaa taccaatcta accattacaa agaccattaa
    10381 ccgtttaggt acagtcttct tcctatttgt cttcaatatt gggatttcca tatttgtatt
    10441 cctattaaca ggaatcatga ttttctcgca ggtacttttt atcatctatg ctatgtttct
    10501 gcctgtgagc tttattttaa gcatgattcc atcatttgat ggtatgtcaa aacgagccat
    10561 aacaaagctc tttaatacca ttttgacacg agctggaatc acattgatta ttacgacagc
    10621 atttagtatt tcaaccatgc tctatacctt atcggctggt tatccgttct ttttgattgc
    10681 ttttctacag attgtgacct ttgcaggaat ctacttcaag ctgggcgatt taatgagtat
    10741 gttttctcta cagagtaacg attctcaaag tgtgggaagt cgtgtgatga gaaaacctcg
    10801 tatgcttatg cacgctcaca tgcaccgtct acagcggaaa cttggacgtt ccatgactac
    10861 tctaggggct gggtctgcca ttgttacagg taaaaaagga cagtcgggtt cggggagttc
    10921 tgcaaggaca caagcagatc actcccgacc agacggaaag gaaaaatcaa cacttggaaa
    10981 acgtatcggt caaaccatcg gtacagtagc tgataccaaa gacagaatgg tagacactgc
    11041 tagtggtttg aaagaacagg ttaaagattt gccgaccaat gcaagatatg cagtatatca
    11101 aggaaaatcc aaagtaaaag agaatgtccg tgatttaacc agtagtattt ctcaaaccaa
    11161 agcggaaaga gccagtggac gcaaggaaca gcaggaacaa aggcgaaaaa ccattgcgaa
    11221 gcgtcgctct gaaatggaac aggtcaaaca gaaaaaacag cctgcttctt ctgttcatga
    11281 aagaccgact acaagacaag aacaatatca tgatgaacag acctcaaaac agtctaatat
    11341 tcagacttca tataaggaat ctcaacaagc caaacaagag cgtccagcag ttaagtccga
    11401 tttttcaagt ccaaaagtgg aacgccaagg caataccgtt caagaaaaaa ccgttcaaaa
    11461 gccagcaact tcaaccacta cagcagatag aacttcacaa cgtccaatca caaaagaacg
    11521 tccgtctact gttcaaagag taccactaca aaatacaaga agtagaccac caatcaaaac
    11581 cgccaccatt aagaaagtcg gtaagaaacc atgaagttga aaactttagt gattggtggt
    11641 tctggattat tcttgatggt cttctcactg cttctgtttg ttgccatttt attttcagat
    11701 gaacaggaca gcggaatttc caatattcat tatggaggtg tgaatgtttc cgcagaagtg
    11761 ctggctcata agcctatggt agaaaaatat gccaaagaat atggcgttga agaatatgtc
    11821 aacatacttc ttgcgattat acaggtggaa tcgggcggta ctgcggaaga tgttatgcag
    11881 tcctcggaat ccctcggtct tccacctaat tcattgagta cagaagaatc cattaagcaa
    11941 ggtgtgaagt atttcagtga attattagcc agtagcgaaa ggctcagtgt agatttagaa
    12001 tcggttatcc agtcctacaa ttatggtggt ggtttcttag ggtatgtggc taatcgtgga
    12061 aataaatata cctttgaact ggctcaaagt ttctcaaaag agtattcagg tggcgaaaaa
    12121 gtgtcttacc ccaatcccat agccatacct atcaatgggg gctggcgata caactatggc
    12181 aatatgtttt atgtgcaact ggtaacgcag tatcttgtca caacagagtt tgatgatgat
    12241 acggtacaag ccatcatgga cgaagcactg aaatatgagg gctggcgata cgtttacggt
    12301 ggagcttccc cgactacttc ttttgattgt agcggactga cacaatggac gtatggaaaa
    12361 gctggaatta acttaccacg aaccgcacaa cagcaatatg atgtgaccca gcatatccca
    12421 ctatcggaag cacaagctgg cgatttggtt ttctttcatt ctacctataa cgctggctct
    12481 tatattactc atgttgggat ataccttggc aataaccgta tgtttcatgc aggcgaccca
    12541 atcggttatg ccgacttaac aagcccctac tggcaacagc atttagtggg agcaggacga
    12601 atcaaacaat gagaaaggaa gatttaatga tgaaatttag aaaaaatcag aataaagaaa
    12661 aacagatacc aaaggaaaag aaacctcgtg tctataaggt caatcctcat aaaaaggttg
    12721 tgattgcctt gtgggtactt ttagggctta gtttcagctt tgcgatattc aagcacttta
    12781 cagctataga tactcatact attcacgaaa caactatcat agaaaaggaa tacgttgata
    12841 ctcatcatgt agaaaatttt gtagagaact ttgcgaaagt ctactattca tgggagcaat
    12901 ccgataagtc cattgataat cgaatggaaa gtctaaaagg ctatctgaca gatgaacttc
    12961 aagctctcaa tgttgataca gtacgcaaag atattcctgt atcgtcttct gtaagaggat
    13021 ttcagatatg gacggtagag ccaactggcg acaatgagtt taatgtaacc tacagtgtag
    13081 accagctcat tacagaggga gaaaatacaa agaccgtcca ctctgcttat atagtgagtg
    13141 tctatgtaga tggttctgga aatatggtac tggttaagaa tccgaccatt accaacatac
    13201 ctaagaaatc aagttataaa ccaaaagcca ttgaaagtga ggggacggtt gattccatta
    13261 caaccaatga aatcaatgag tttttaacga cgttcttcaa gctctatcct acagcgacag
    13321 ccagtgaact ttcctactat gtgaatgacg ggatattaaa accaatcgga aaagagtaca
    13381 tctttcaaga actggtaaat cctattcaca atcgtaagga taatcaagtc acggtatcgc
    13441 tgacagtgga gtatatcgac cagcagacca aagcaacgca ggtatctcaa tttgatttgg
    13501 tacttgaaaa gaacgggagt aattggaaga ttatagaata acaaatattg gtacattatt
    13561 acagctattt tgtaatcacg tactctcttt gataaaaaat tggagattcc tttacaaata
    13621 tgctcttacg tgctattatt taagtatcta tttaaaagga gttaataaat atgcggcaag
    13681 gtattcttaa ataaactgtc aatttgatag tgcgaacaaa taattggatg tcctttttta
    13741 ggagggctta gttttttgta cccagtttaa gaataccttt atcatgtgat tctaaagtat
    13801 ccggagaata tctgtatgct ttgtatgcct atggttatgc ataaaaatcc cagtgataag
    13861 agtatttatc actgggattt ttatgccctt ttgggctttt gaatggagga aaatcacatg
    13921 aaaattatta atattggagt tttagctcat gttgatgcgg gaaaaactac cttaacagaa
    13981 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
    14041 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
    14101 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
    14161 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
    14221 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
    14281 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
    14341 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
    14401 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
    14461 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
    14521 caagaggaaa gcataagatt tcataattgt tccctgttcc ctgtttatca cggaagtgca
    14581 aaaaacaata tagggattga taaccttata gaagtgatta cgaataaatt ttattcatca
    14641 acacatcgag gtcagtctga actttgcgga aaagttttca aaattgagta ttcggaaaaa
    14701 agacagcgtc ttgcatatat acgtctttat agtggcgtac tgcatttgcg agattcggtt
    14761 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
    14821 ttatgtaaaa tcgataaggc ttattccggg gaaattgtta ttttgcagaa tgagtttttg
    14881 aagttaaata gtgttcttgg agatacaaag ctattgccac agagagagag aattgaaaat
    14941 cccctccctc tgctgcaaac aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta
    15001 cttgatgcac ttttagaaat ctccgacagt gacccgcttc tgcgatatta tgtggattct
    15061 gcgacacatg aaatcatact ttctttctta gggaaagtac aaatggaagt gacttgtgct
    15121 ctgctgcaag aaaagtatca tgtggagata gaaataaaag agcctacagt catttatatg
    15181 gaaagaccgt taaaaaaagc agagtatacc attcacatcg aagttccacc gaatcctttc
    15241 tgggcttcca ttggtttatc tgtatcaccg cttccgttgg gaagtggaat gcagtatgag
    15301 agctcggttt ctcttggata cttaaatcaa tcatttcaaa atgcagttat ggaagggata
    15361 cgctatggct gtgaacaagg attgtatggt tggaatgtga cggactgtaa aatctgtttt
    15421 aagtatggct tatactatag ccctgttagt accccagcag attttcggat gcttgctcct
    15481 attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc atatcttagt
    15541 tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaccgatgc tcctaaatat
    15601 tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag tggagaaatc
    15661 cctgctcggt gtattcaaga atatcgtagt gatttaactt tctttacaaa tggacgtagt
    15721 gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt ttgccagccc
    15781 cgtcgtccaa atagtcggat agataaagta cgatatatgt tcaataaaat aacttagtgt
    15841 attttatgtt gttatataaa tatggtttct tgttaaataa gatgaaatat tttttaataa
    15901 agatttgaat taaagtgtaa aggaggagat agttattata aactacaagt ggatattgtg
    15961 tgctgagagc tttcttctat actaatagac gaaagggtgt gaaaatgatt tttaaatgat
    16021 actgtggaac ggaacagtag ccctagtatt gactactgtc gtttctattc atattggcta
    16081 ttctaggact gagatgaaaa aatctataaa tgctcagaat aaaattgaac ccgcaaatct
    16141 ccccaaaaca atggtgagtc atgtacttgt attattccga aaaaatacac ctctggtgca
    16201 gtgagacaaa ttggtgtatc ttatagtggc ttcgtagatg aaagctatac tctactatca
    16261 ctctttgatg atgtagaaca aattgaaaaa gataatagac ttcagacagc tattgatgtt
    16321 gtcagagaac agtttggttt tttagccata caaaaaggaa ccgtcctaac tgaaggttcc
    16381 agaaatattg aacgcagtaa acttatcggt ggtcattccg cgggtggatt ggagggatta
    16441 aaatgaaaca agaaaaaaat acagtacaat tttcagaaat ccgtagcaaa ggatgtaatg
    16501 atattgaaat gcttgaaaga tttttacatg gaatcgttga aacagcaact tcaaaacttc
    16561 gtcagagaaa actcaaaaca actgaaatat cgatacgact agtacatgct aaatctgaaa
    16621 accgattacc attggaattt acatttagca ttaagccaac aagctcatct gtgataatct
    16681 atactgaggt aatcaatcgc tttaaagaat gttacacagg tgggggaatt caaggtttta
    16741 cgattcaatt tgataaaaat acccttgcct ctgcatagaa aggatttgat atgattgacc
    16801 gttcatattt accatttcaa tcagcaagag agtaccagga tacaaagatg caaaaatgga
    16861 tgggcttttt cctatctgaa catgcatcag cactctctga tgatacaaac aaagtaacgt
    16921 acatgtctga cttatcacta gagaagaaat tattactcct cagtcaagta tacgccgggc
    16981 agctacgcac acgcattcaa gtgattgaaa aaaacaagcg tgtttcctac actggaacaa
    17041 taccaagtct gaccaaagat ttcattttga taaaaactac aacaggtcac atcaatttga
    17101 aattaaaaga cattattagt attgaacttg tcgaggaggt gctctatgaa tcagcttgag
    17161 tttcagcgta atcacctaca aatggactat tatagcgaga gctaccaaga ttttgaacgt
    17221 gacttctacc gctactctaa catgaatatt ccattgacct tcctaactga tgatatccta
    17281 aaaacaatgg cgacttcacg taagaattac tttgtcctca ataaggaaaa gtccagagat
    17341 aaccgcgatc acttcttcat atttgaagta agtaccgtag atgagaatcc gctaatctat
    17401 cattatacat ataagaaaac tacaatatat ttagcagaaa aataggagca gttcaattga
    17461 ctgttcctat ttttaatatt cataaaatct aaagtcttta tactctttaa caatggagtc
    17521 gccaaccaga acagactata ctgaccagcg actaccttaa atttaatgtt tcagatttat
    17581 tttcttatct ctaatttcat aaactacatc tgctacattt tcgagtaatc gtttatcgtg
    17641 ggtgataaac acgatagttc cggtgtactc cttcattagt atttccaaag cctctaaact
    17701 tggtatgtca aggaagttac tgggttcatc cattattagg atgttatatc tacccatgag
    17761 cattttagca agcaacaatt ttataatttc tccaccgctt aaaacagata aactttttcc
    17821 aatatcgttc tgtttgaacc ccatagatgc tagcactgaa cgaatttctg atatattgta
    17881 gtcacaatcc ttctgcataa actccataac attctgatta ctgttgtact tgtaaccatt
    17941 ctgtgcaaag taacctattt ttgccttagg cgaaatagaa attccttctt catggtttaa
    18001 gatcatttgg attaaagttg tttttccgat tccattacca ccagttaacg ccacttttgc
    18061 tcctaacgga atttgaaaag atgcattttc aaacagagcc ttatccccaa atactttatt
    18121 aatttctgca ccgactatag ggtatggatt atggagctcc aatgctttac tttgcctgaa
    18181 acgaattctg cgaatgcctt ccggagcttc tacttttcct aaggccgcaa tcctgtgctc
    18241 tagggtttta gcagcattat acatcttttt ttccttactt cctattgatt tttgatgagc
    18301 taaacgccct ccgtcttcag tacttttttt ctttgaagaa ccttttgcct tctgttctat
    18361 tttacgagcc tgttttcgct tttcctccgc agccctttcc aatcgggcac gttccgcaat
    18421 aaattgttcg tattctgcag cttggctctt acgttcttcc tctttctgac gaagataatc
    18481 agaatagttt ccccaatact cagtgatttt gccatctttc agttcccata ttttatctac
    18541 tatttcatca agaaaatagc ggtcatggct aataactaac agtgcacctg taaaatattt
    18601 tagctgtcct attagaaaat caattccttc acggtctaaa tggctcgtag gttcatccgc
    18661 taaaatacca tgaacctgtg ccgataaggc ctgtgctatt ttaagccttg tttcttcacc
    18721 accgctcata gtctgtatat ttaattgctc aacacctagc ttgcctacaa gtgcaaaatc
    18781 tttttcctcc tgcagagtta cttcgtccaa ctggggaata taggcaagtt cacccagacg
    18841 attcatttta catcctgggg gagttaattc tcctaaaagt accctgagta aagtgctttt
    18901 tccagcacca tttgctccta ctaaaccaat acggtcataa tcatatactt ctaattcatt
    18961 tatatctaaa acatcgcgtc ctttgaattc cacacgaatg tcttttgctt ttaatattaa
    19021 ttccataaca tttcctcctg tctataatcg catgctttca tttgcttgta tgcagggaaa
    19081 accctgcgat tttagcagga agagttacat gaaaataaga tacataaata ttcctccaat
    19141 attgtttatt ttaaatctaa ttttctaacc tcagttatca tttggcaaac tatagcaatg
    19201 ccaataatta aaatacctga tagtaaaaac caatgattta caccgatttt atcagcaaag
    19261 aatccagaaa gaattaaccc aattggcata gcaagtgaca tgatacttcc gatcaaagaa
    19321 aatacacgtc ctaaatattc aggcttaatt ttctcctgaa aaagagctgt ttgcacaccg
    19381 ctataaaatg gcaccgaaag ccccattatt gcacagcaaa ctacgaatat tacaaatcca
    19441 tttggaggaa gtattcccga aacggctaaa ctggtcccca ttataaaaaa tgaacttgtt
    19501 attagtaata catgcttttc gaagccccct aatcttccta ataataagcc tcctgctagc
    19561 atcccaaatg caaaggaaat ttccgtaata gaaatatgca caggcgttcc attaaagtgt
    19621 tccatgctta ttaaaggaaa tagtgcattg attggcatat aaacaaaagt atatagtgtt
    19681 cctaagagta ataaggcaaa caatcctttg ttttgtctca gaaccacaac tccttctttc
    19741 atctccctta tgaaatttgg ttctaaactt tgcacttgat tacccagctt aggtatacgt
    19801 acaattgcta ccgtaataga tgcaatcaca gcacccaata cgtcgatggc aataatagca
    19861 tttaaatccc aaacggagta taagagtgct gcaactgccg gactaacaat atagcttata
    19921 gactgcaaag actgactata gcctgcgcat ttcgttagct gttcttctgg tactaaaagt
    19981 ggtgtaaccg cattgagtgc tggggtatga aaagctgttc caatgctacg gataaacaat
    20041 actatcataa tcatccagac aggtagctcc atacagaatg caacaatagc aagcactgca
    20101 ccagctgctg cgataattaa atcggcacca atcattatct tcttcctatc atgacgatcc
    20161 actagcacac caatggcagg tcccaa
//
LOCUS       DAVAAL010000254         5319 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.guided.121, whole genome shotgun sequence.
ACCESSION   DAVAAL010000254 DAVAAL010000000
VERSION     DAVAAL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.guided.121"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            263..1480
                     /gene="mef(A)"
                     /locus_tag="VQM60_002103"
     CDS             263..1480
                     /gene="mef(A)"
                     /locus_tag="VQM60_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HET1081905.1"
                     /translation="MEKYNNWKRKFYAIWAGQAVSLITSAILQMAIIFYLTEKTGSAM
                     VLSMASLVGFLPYAILGPAIGVLVDRHDRKKIMIGADLIIAAAGAVLAIVAFCMELPV
                     WMIMIVLFIRSIGTAFHTPALNAVTPLLVPEEQLTKCAGYSQSLQSISYIVSPAVAAL
                     LYSVWDLNAIIAIDVLGAVIASITVAIVRIPKLGNQVQSLEPNFIREMKEGVVVLRQN
                     KGLFALLLLGTLYTFVYMPINALFPLISMEHFNGTPVHISITEISFAFGMLAGGLLLG
                     RLGGFEKHVLLITSSFFIMGTSLAVSGILPPNGFVIFVVCCAIMGLSVPFYSGVQTAL
                     FQEKIKPEYLGRVFSLIGSIMSLAMPIGLILSGFFADKIGVNHWFLLSGILIIGIAIV
                     CQMITEVRKLDLK"
     gene            1600..3063
                     /gene="msr(D)"
                     /locus_tag="VQM60_002104"
     CDS             1600..3063
                     /gene="msr(D)"
                     /locus_tag="VQM60_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(D)"
                     /protein_id="HET1081906.1"
                     /translation="MELILKAKDIRVEFKGRDVLDINELEVYDYDRIGLVGANGAGKS
                     TLLRVLLGELTPPGCKMNRLGELAYIPQLDEVTLQEEKDFALVGKLGVEQLNIQTMSG
                     GEETRLKIAQALSAQVHGILADEPTSHLDREGIDFLIGQLKYFTGALLVISHDRYFLD
                     EIVDKIWELKDGKITEYWGNYSDYLRQKEEERKSQAAEYEQFIAERARLERAAEEKRK
                     QARKIEQKAKGSSKKKSTEDGGRLAHQKSIGSKEKKMYNAAKTLEHRIAALGKVEAPE
                     GIRRIRFRQSKALELHNPYPIVGAEINKVFGDKALFENASFQIPLGAKVALTGGNGIG
                     KTTLIQMILNHEEGISISPKAKIGYFAQNGYKYNSNQNVMEFMQKDCDYNISEIRSVL
                     ASMGFKQNDIGKSLSVLSGGEIIKLLLAKMLMGRYNILIMDEPSNFLDIPSLEALEIL
                     MKEYTGTIVFITHDKRLLENVADVVYEIRDKKINLKH"
     gene            complement(3181..3480)
                     /locus_tag="VQM60_002105"
     CDS             complement(3181..3480)
                     /locus_tag="VQM60_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081907.1"
                     /translation="MNQLEFQRNHLQMDYYSESYQDFERDFYRYSNMNIPLTFLTDDI
                     LKTMATSRKNYFVLNKEKSRDNRDHFFIFEVSTVDENPLIYHYTYKKTTIYLAEK"
     gene            complement(3467..3835)
                     /locus_tag="VQM60_002106"
     CDS             complement(3467..3835)
                     /locus_tag="VQM60_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081908.1"
                     /translation="MIDRSYLPFQSAREYQDTKMQKWMGFFLSEHASALSDDTNKVTY
                     MSDLSLEKKLLLLSQVYAGQLRTRIQVIEKNKRVSYTGTIPSLTKDFILIKTTTGHIN
                     LKLKDIISIELVEEVLYESA"
     gene            complement(3848..4183)
                     /locus_tag="VQM60_002107"
     CDS             complement(3848..4183)
                     /locus_tag="VQM60_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imsC-like protein"
                     /protein_id="HET1081909.1"
                     /translation="MKQEKNTVQFSEIRSKGCNDIEMLERFLHGIVETATSKLRQRKL
                     KTTEISIRLVHAKSENRLPLEFTFSIKPTSSSVIIYTEVINRFKECYTGGGIQGFTIQ
                     FDKNTLASA"
     gene            complement(4180..4425)
                     /locus_tag="VQM60_002108"
     CDS             complement(4180..4425)
                     /locus_tag="VQM60_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081910.1"
                     /translation="MRQIGVSYSGFVDESYTLLSLFDDVEQIEKDNRLQTAIDVVREQ
                     FGFLAIQKGTVLTEGSRNIERSKLIGGHSAGGLEGLK"
     gene            complement(4789..>5319)
                     /locus_tag="VQM60_002109"
     CDS             complement(4789..>5319)
                     /locus_tag="VQM60_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET1081911.1"
                     /translation="VSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYY
                     SPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANI
                     VDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRR
                     PNSRIDKVRYMFNKIT"
BASE COUNT         1673 a          884 c         1050 g         1712 t
ORIGIN      
        1 caataaaaaa agcagaatct atacccgatg ataggctttt ttgttgtgct tatttatacg
       61 atattgagca ttcattagtt acggtgagga tattggttat ttaactatac ctttatttaa
      121 ctatgtcttt aatatgaatg tttccaaatt gtatgtatgc agaccaaaag ccacattgtg
      181 gggtttggcc tgcatttttt attgcctaga atgctattca aaatagaaat tcaagcaaaa
      241 taatatgcag gagataatat aaatggaaaa atacaacaat tggaaacgaa aattttatgc
      301 aatatgggca gggcaagcag tatcattaat cactagtgcc atcctgcaaa tggcgattat
      361 tttttacctt acagaaaaaa caggatctgc gatggtcttg tctatggctt cattagtagg
      421 ttttttaccc tatgcgattt tgggacctgc cattggtgtg ctagtggatc gtcatgatag
      481 gaagaagata atgattggtg ccgatttaat tatcgcagca gctggtgcag tgcttgctat
      541 tgttgcattc tgtatggagc tacctgtctg gatgattatg atagtattgt ttatccgtag
      601 cattggaaca gcttttcata ccccagcact caatgcggtt acaccacttt tagtaccaga
      661 agaacagcta acgaaatgcg caggctatag tcagtctttg cagtctataa gctatattgt
      721 tagtccggca gttgcagcac tcttatactc cgtttgggat ttaaatgcta ttattgccat
      781 cgacgtattg ggtgctgtga ttgcatctat tacggtagca attgtacgta tacctaagct
      841 gggtaatcaa gtgcaaagtt tagaaccaaa tttcataagg gagatgaaag aaggagttgt
      901 ggttctgaga caaaacaaag gattgtttgc cttattactc ttaggaacac tatatacttt
      961 tgtttatatg ccaatcaatg cactatttcc tttaataagc atggaacact ttaatggaac
     1021 gcctgtgcat atttctatta cggaaatttc ctttgcattt gggatgctag caggaggctt
     1081 attattagga agattagggg gcttcgaaaa gcatgtatta ctaataacaa gttcattttt
     1141 tataatgggg accagtttag ccgtttcggg aatacttcct ccaaatggat ttgtaatatt
     1201 cgtagtttgc tgtgcaataa tggggctttc ggtgccattt tatagcggtg tgcaaacagc
     1261 tctttttcag gagaaaatta agcctgaata tttaggacgt gtattttctt tgatcggaag
     1321 tatcatgtca cttgctatgc caattgggtt aattctttct ggattctttg ctgataaaat
     1381 cggtgtaaat cattggtttt tactatcagg tattttaatt attggcattg ctatagtttg
     1441 ccaaatgata actgaggtta gaaaattaga tttaaaataa acaatattgg aggaatattt
     1501 atgtatctta ttttcatgta actcttcctg ctaaaatcgc agggttttcc ctgcatacaa
     1561 gcaaatgaaa gcatgcgatt atagacagga ggaaatgtta tggaattaat attaaaagca
     1621 aaagacattc gtgtggaatt caaaggacgc gatgttttag atataaatga attagaagta
     1681 tatgattatg accgtattgg tttagtagga gcaaatggtg ctggaaaaag cactttactc
     1741 agggtacttt taggagaatt aactccccca ggatgtaaaa tgaatcgtct gggtgaactt
     1801 gcctatattc cccagttgga cgaagtaact ctgcaggagg aaaaagattt tgcacttgta
     1861 ggcaagctag gtgttgagca attaaatata cagactatga gcggtggtga agaaacaagg
     1921 cttaaaatag cacaggcctt atcggcacag gttcatggta ttttagcgga tgaacctacg
     1981 agccatttag accgtgaagg aattgatttt ctaataggac agctaaaata ttttacaggt
     2041 gcactgttag ttattagcca tgaccgctat tttcttgatg aaatagtaga taaaatatgg
     2101 gaactgaaag atggcaaaat cactgagtat tggggaaact attctgatta tcttcgtcag
     2161 aaagaggaag aacgtaagag ccaagctgca gaatacgaac aatttattgc ggaacgtgcc
     2221 cgattggaaa gggctgcgga ggaaaagcga aaacaggctc gtaaaataga acagaaggca
     2281 aaaggttctt caaagaaaaa aagtactgaa gacggagggc gtttagctca tcaaaaatca
     2341 ataggaagta aggaaaaaaa gatgtataat gctgctaaaa ccctagagca caggattgcg
     2401 gccttaggaa aagtagaagc tccggaaggc attcgcagaa ttcgtttcag gcaaagtaaa
     2461 gcattggagc tccataatcc ataccctata gtcggtgcag aaattaataa agtatttggg
     2521 gataaggctc tgtttgaaaa tgcatctttt caaattccgt taggagcaaa agtggcgtta
     2581 actggtggta atggaatcgg aaaaacaact ttaatccaaa tgatcttaaa ccatgaagaa
     2641 ggaatttcta tttcgcctaa ggcaaaaata ggttactttg cacagaatgg ttacaagtac
     2701 aacagtaatc agaatgttat ggagtttatg cagaaggatt gtgactacaa tatatcagaa
     2761 attcgttcag tgctagcatc tatggggttc aaacagaacg atattggaaa aagtttatct
     2821 gttttaagcg gtggagaaat tataaaattg ttgcttgcta aaatgctcat gggtagatat
     2881 aacatcctaa taatggatga acccagtaac ttccttgaca taccaagttt agaggctttg
     2941 gaaatactaa tgaaggagta caccggaact atcgtgttta tcacccacga taaacgatta
     3001 ctcgaaaatg tagcagatgt agtttatgaa attagagata agaaaataaa tctgaaacat
     3061 taaatttaag gtagtcgctg gtcagtatag tctgttctgg ttggcgactc cattgttaaa
     3121 gagtataaag actttagatt ttatgaatat taaaaatagg aacagtcaat tgaactgctc
     3181 ctatttttct gctaaatata ttgtagtttt cttatatgta taatgataga ttagcggatt
     3241 ctcatctacg gtacttactt caaatatgaa gaagtgatcg cggttatctc tggacttttc
     3301 cttattgagg acaaagtaat tcttacgtga agtcgccatt gtttttagga tatcatcagt
     3361 taggaaggtc aatggaatat tcatgttaga gtagcggtag aagtcacgtt caaaatcttg
     3421 gtagctctcg ctataatagt ccatttgtag gtgattacgc tgaaactcaa gctgattcat
     3481 agagcacctc ctcgacaagt tcaatactaa taatgtcttt taatttcaaa ttgatgtgac
     3541 ctgttgtagt ttttatcaaa atgaaatctt tggtcagact tggtattgtt ccagtgtagg
     3601 aaacacgctt gtttttttca atcacttgaa tgcgtgtgcg tagctgcccg gcgtatactt
     3661 gactgaggag taataatttc ttctctagtg ataagtcaga catgtacgtt actttgtttg
     3721 tatcatcaga gagtgctgat gcatgttcag ataggaaaaa gcccatccat ttttgcatct
     3781 ttgtatcctg gtactctctt gctgattgaa atggtaaata tgaacggtca atcatatcaa
     3841 atcctttcta tgcagaggca agggtatttt tatcaaattg aatcgtaaaa ccttgaattc
     3901 ccccacctgt gtaacattct ttaaagcgat tgattacctc agtatagatt atcacagatg
     3961 agcttgttgg cttaatgcta aatgtaaatt ccaatggtaa tcggttttca gatttagcat
     4021 gtactagtcg tatcgatatt tcagttgttt tgagttttct ctgacgaagt tttgaagttg
     4081 ctgtttcaac gattccatgt aaaaatcttt caagcatttc aatatcatta catcctttgc
     4141 tacggatttc tgaaaattgt actgtatttt tttcttgttt cattttaatc cctccaatcc
     4201 acccgcggaa tgaccaccga taagtttact gcgttcaata tttctggaac cttcagttag
     4261 gacggttcct ttttgtatgg ctaaaaaacc aaactgttct ctgacaacat caatagctgt
     4321 ctgaagtcta ttatcttttt caatttgttc tacatcatca aagagtgata gtagagtata
     4381 gctttcatct acgaagccac tataagatac accaatttgt ctcactgcac cagaggtgta
     4441 ttttttcgga ataatacaag tacatgactc accattgttt tggggagatt tgcgggttca
     4501 attttattct gagcatttat agattttttc atctcagtcc tagaatagcc aatatgaata
     4561 gaaacgacag tagtcaatac tagggctact gttccgttcc acagtatcat ttaaaaatca
     4621 ttttcacacc ctttcgtcta ttagtataga agaaagctct cagcacacaa tatccacttg
     4681 tagtttataa taactatctc ctcctttaca ctttaattca aatctttatt aaaaaatatt
     4741 tcatcttatt taacaagaaa ccatatttat ataacaacat aaaatacact aagttatttt
     4801 attgaacata tatcgtactt tatctatccg actatttgga cgacggggct ggcaaacagg
     4861 ttcaccggta gtaacatggt acccttttaa ctctgttaaa caaacactac gtccatttgt
     4921 aaagaaagtt aaatcactac gatattcttg aatacaccga gcagggattt ctccactaag
     4981 aatgacctca ttatttttca attgagtgtc tacgatgttc gcacaatatt taggagcatc
     5041 ggtgtatgct cgtgaaagat attcctgtgg cgcataaatt ttaaaactaa gatatggctc
     5101 taacaattct gttccagctt tttttaagac ttgttccaat acaataggag caagcatccg
     5161 aaaatctgct ggggtactaa cagggctata gtataagcca tacttaaaac agattttaca
     5221 gtccgtcaca ttccaaccat acaatccttg ttcacagcca tagcgtatcc cttccataac
     5281 tgcattttga aatgattgat ttaagtatcc aagagaaac
//
LOCUS       DAVAAL010000255         2293 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MD5261
            SAMN08647617-rid20187553.guided.177, whole genome shotgun sequence.
ACCESSION   DAVAAL010000255 DAVAAL010000000
VERSION     DAVAAL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08647617
            Sequence Read Archive: SRR6818235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001981138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001981138.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 03:21:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,991
            CDSs (with protein)               :: 1,991
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 50 of 85
            Pseudo Genes (incomplete)         :: 31 of 85
            Pseudo Genes (internal stop)      :: 24 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08647617-rid20187553.guided.177"
                     /strain="MD5261"
                     /serotype="19A"
                     /isolation_source="nasopharyngeal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="USA"
                     /lat_lon="42.36 N 71.399 W"
                     /collection_date="2009"
                     /collected_by="HARVARD PILGRIM HEALTH CARE"
     gene            31..1119
                     /locus_tag="VQM60_002110"
                     /pseudo
     CDS             31..1119
                     /locus_tag="VQM60_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849941.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
     gene            1394..1696
                     /locus_tag="VQM60_002111"
     CDS             1394..1696
                     /locus_tag="VQM60_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET1081912.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKLVSINE"
     gene            1752..2180
                     /locus_tag="VQM60_002112"
     CDS             1752..2180
                     /locus_tag="VQM60_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET1081913.1"
                     /translation="MDGITKDFIKTAKLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFT
                     EVEGDTIVGLALCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAK
                     NKGCKEFASDCTLTNTDSIRFHLNIGFQEANRIIHFKKKL"
BASE COUNT          799 a          332 c          478 g          684 t
ORIGIN      
        1 cacaagtaca atacgacaga ttactttgaa attgaccgtc attttggaga caaggagacc
       61 tttcgggaac tggtggatca agcgcatcat cgtggcatga aagtcatgct ggatgcggta
      121 tttaatcata ttggttcgca atctcttcaa tggaaaaatg tcgtcaaaaa tggtgaacag
      181 tctgcttata aggattggtt ccatattcaa caattcccag tgacaactga aaagctagtt
      241 aataagagag acttacccta tcatgttttt ggtttcgagg actatatgcc taagctaaat
      301 acagccaatc cagaggtcaa gaattatctt ttaaaggttg cgacttattg gattgaagag
      361 tttaatatcg atgcttggcg tttggatgtg gctagtgaga ttgaccatca gttctggaag
      421 gattttcgta aggcagtttt agctaaaaat cctgatcttt atatcctagg agaagtctgg
      481 catacatctc agccttggct aaatggagat gagttccatg ccgtcatgaa ttatccttta
      541 tctgatagta tcaaggacta tttcttacga ggaattaaga agacagacca gttcatcgat
      601 gaaatcaatt gagagtctat gtattacaag cagcagattt cagaggtcat gtttaatctc
      661 ttggattcac atgatacaga gcgaatcctg tggacggcca atgaagatgt tcaactggtt
      721 aaatcagcct tagcctttct cttttaacaa aaaggaacac cgtgcattta ttacggaacc
      781 gagctagcct tgactggagg accagatcca gattgtcgtc gttgtatgcc ttgggaacgt
      841 gtatcaagtg acaatgatat gctgaacttt atgaagaggc tgattaagat tcggaaatac
      901 gcgtcagtaa tcatttcgca tggcaagtat agccttcaag aaatcaaatc tgatctagta
      961 gctctggaat ggaaatacga aggatggatc ctcaaagcaa tattcaacca atcaacagaa
     1021 gattatcttt tagagaaaga agcagtagca ctagcaagca attgccaaga attggagaat
     1081 cagcttgtca tctctccaga tggatttgtg attttctaaa aactagttga tgaagattat
     1141 ggtacatttc atatcttata tagtataata aggctagtta ctaaacttgt aaaggagaac
     1201 ttaaatgaat tgtagaggac atgaaacaag acaaagaatt gttagagatt ttgaagttta
     1261 gcctaaagca catattaagc tgttagcaaa tcaacaaaaa catagtgatg caggagcaac
     1321 tattgaagat gaatattatg tatttatcgc tgagagtaaa attgatggca agaaggaagt
     1381 tattcagtgt tgcatgggtg cggcaaggga ttttttagaa ctaattaatc acaaagggct
     1441 acctcttttt aatccgcttg taggtgattc tcatgtaaat aatagacaag aatatgacaa
     1501 tacagggagt ggaaatttac aacctgaaaa gtggaatgaa actgcaaagc agctttataa
     1561 tgttataatg tggttgatta ttttatggaa tgctaagccg gatacacctt tatttaattt
     1621 taaagacgaa gtaattaagt ataaaacata tgagcctttt gaaagcagta taaaattggt
     1681 atcaatcaat gagtaacaaa gtatcagcat gattcgaaac atttacttgt aatttgcgga
     1741 ggaacatcaa tatggatgga ataacaaaag attttataaa aacggctaaa ctaatgaaac
     1801 aattatggcc ccaattgacc gataaagaag ctattgatga agtaaaaaaa tatacgaatg
     1861 gcaaaaatac tgcaatcttt actgaagttg aaggtgacac aattgtaggt ctagcactat
     1921 gttcactcag atttgattat gttgaaggtt gtaaatatag tcctgttgga ttcttagaag
     1981 ggattattgt tgacgaggaa tatcgtttaa aggatattgc taaaaatctc tgtacaaaat
     2041 gtgaggaatg ggcgaaaaat aaaggatgta aggaatttgc aagtgactgt actttaacga
     2101 atacggattc tataagattt catctcaata ttggatttca ggaggcaaat agaattattc
     2161 attttaagaa aaaattataa tttagaaacg tgaataaact tgtttaaact ttatgtatct
     2221 ttagaaagtg tgttaacttg aaagaacaaa tttttttgat gggtgggaat cccccaatga
     2281 agaaatatag tat
//
